KR20070091101A - Incising cell to basement membrane bonds - Google Patents

Abstract

Cells are attached to each other and to a basement membrane, to form a layer or layers. Cells may be separated from basement membrane without damaging the cells or basement membrane by the devices disclosed here. The devices enable simultaneous exposure of the cell basement membrane complex to light energy from both sides, the cells side and the basement membrane side. This simultaneous exposure of the cell basement membrane complex layer to specific levels of light energy from two sides causes incision of the bonds that attach the cells to the basement membrane.

Description

Incision of cell binding to basement membrane {INCISING CELL TO BASEMENT MEMBRANE BONDS}

Disclosed is a device for cutting the binding between a cell and a basement membrane without damaging the cell or basement membrane of the present invention.

The device cleaves the bond between the cell and the basement membrane against the cell basement membrane complex by exposing it to very low intensity light energy coming in two directions on the cell side and at a higher intensity on the basement membrane side.

The effect of light on the cell basement membrane complex depends on the wavelength, intensity, exposure time, intrinsic composition of the tissue upon exposure, and the direction of exposure. The present specification deals with a method of specifically cutting the basement membrane-bound cells by exposing light having a particular wavelength to light of very small intensity on the cell side, and at the same time to light of more intense intensity on the basement membrane side.

In the laboratory or in various surgical procedures, there is a need to effectively isolate cells attached to the basement membrane or capsules for various reasons. This isolation is intended to prevent the involvement or deterioration of the basement membrane or tissue, to further visualize the structure or tissue behind the cell or basement membrane, and to study better pigmentation for cell imaging, and to study cellular properties, for example. It must be performed effectively to achieve optical advantages such as better manipulation.

The prior art discloses several devices and methods for isolating cells attached to cell membranes.

The present invention is directed to an apparatus and method for solving various problems associated with the prior art. The present invention implements low intensity light emitting devices having a selected wavelength to separate epithelial cells. The device allows the operator to expose the cell basement membrane complex to light energy incident from two directions, thereby achieving the intended effect of isolating epithelial cells from the basement membrane to which they are attached. The effect is achieved by cleaving the bond between the cell and the basement membrane.

The device can be used in a variety of therapeutic, experimental, and scientific processes.

Within the human body and in the laboratory, it is common to see cells arranged in a single layer or in multiple layers and arranged in a line on the basement membrane. For example, epithelial cells (epithelium) on the corneal surface of the human eye are arranged on the basement membrane, which in turn is called the Bowman's membrane, forming four to six layers. The binding between the cells and the basement membrane is very strong. These epithelial cells are very resistant to light incident from the outside. However, studies have shown that the adhesion of cells to the basement membrane is very fragile and susceptible to light energy when irradiated with weak light from the inside and at the same time with strong light from the outside.

In mammalian eyes, removal of the remaining portion of the lens material during cataract surgery shows that the lens epithelial cells proliferate. These cells are opaque and can cause cataract sequelae that affect vision. Some of these cells may change their properties after surgery to become fibroblasts or to form fibrous scars in the capsule, leading to capsular contraction syndrome. Although these cells do not cause this problem, they cause turbidity in the capsule, which in turn hinders the visualization of the tissue. This problem makes the treatment and examination of the retina difficult for optical reasons.

It is desirable to remove these cells during cataract surgery to avoid these problems after surgery.

Cell membranes such as ocular membranes are very thin and weak. The space in which the surgery is performed is very limited and the coating must be kept intact with the surrounding tissue. The internal tissue of the eye is not resistant to high energy impacts such as chemicals, heat, electricity, lasers, mechanical friction, and the like.

Lens epithelial cells attach to the capsule from the inside. The adhesion between the cells and the membrane is so strong that simple washing does not drop it. If this attachment is loose or detached, the cells can be easily washed off or aspirated by a simple tubular wash tube attached to the syringe.

These cells cannot be removed by a laser device. This is because, with the laser device, the cells die and the dead cells can stick to the film and cause optical problems after surgery.

The prior art in the art discloses various means for overcoming the problems associated with removing such epithelial cells.

Some prior art discloses methods using mechanical means to remove unwanted cells. The main limitation of these methods is that they can damage surrounding tissues.

International Patent Publication No. WO 00/49976, PCT / US00 / 04339, describes a Nicapsulorhexis Valve. It is a silicone valve that attaches to the encapsulant hex opening in a waterproof manner. This prevents the rest of the inner surface of the eye from coming into contact with any cytotoxic substances that can enter the capsular bag and destroy the epithelial cells.

International Patent Publication No. WO 99/04729 deals with an intraocular ring device. The document deals with a physical device called an eye ring that kills or prevents proliferation of cells by the compressive effect of contact with the cells.

International Patent Publication No. WO 2004/039295 describes a method of making encapsulated hexesis in a capsular coating. The lens is removed from the capsular coating of the eye and the capsular hexis is sealed by a sealing means / apparatus to provide a gas leak-proof seal. The capsular film is expanded with gas, and the desired surgery is performed in the expanded capsular film.

Here, the inventors disclose a method in which an airtight device seals the capsular bag from the rest of the eye so that toxic gases or liquids enter the bag and kill the cells.

US Pat. No. 6,432,078 describes a system and method for removing cataracts or other cells in the eye using a water jet and suction. This discloses a mechanical device for scrubbing cells from the eye using a water jet, a mechanical brush or the like.

International Patent Publication No. WO 98/25610, PCT / CA97 / 00949 discloses the use of green porphyrins for the manufacture of a medicament for the treatment of secondary cataracts. In this document, researchers at the University of Colombia disclose a chemical called green porphyrin. The chemical is applied to epithelial cells and then irradiated with light, which results in the destruction of the cells to which the substance is applied. This is called photodynamic therapy of the capsular film. Porphyrin is a chemical and must be introduced into the eye, so the method is not preferred.

International Patent Publication No. WO 99/39722, PCT / IB99 / 00905, discloses compositions and methods for isolating lens epithelial cells and preventing subsequent clouding of the coating. This is a focal contact that modulates adhesion between the lens epithelial cell and the capsular membrane using a treatment solution containing a proenzyme or focal contact modulator, such as lys-plasminogen, which enters the eye. This can be achieved by adjusting the contact.

International Patent Publication No. WO 02/047728, PCT / GB01 / 05465, discloses a method for treating subsequent encapsulation of the film. The document deals with methods of killing cells using chemical ligands. The ligand is preferably a Fas ligand and the spacer is polyethylene glycol. Preferably the polymer constitutes an intraocular lens.

International Patent Publication No. WO 02/43632, PCT / AU01 / 01554, discloses a device for sealing an ocular capsular bag and a method for delivering a fluid or therapeutic material to the ocular lens. Also disclosed are methods of delivering powerful chemicals to seal the capsular bag from the rest of the eye and simultaneously kill cells.

US Pat. No. 4,966,577 discloses compositions that prevent secondary cataract formation after lens removal. The composition is specific for particular lens cells involved in secondary cataract formation and includes antibodies that conjugate to antiproliferative substances. Particularly preferred antiproliferative substances require activation after the antibody binds to the subject cell, which activation can be accomplished by addition of a second composition or exposure of the eye to electromagnetic energy. Also disclosed is a method of using the composition by administering the composition directly to the point where the lens is removed to kill or prevent proliferation of the lens cells.

The document again details the introduction of the original chemicals and then describes the introduction of other chemicals and the activation of their binding using electromagnetic energy to destroy the capsular cells.

US Pat. Nos. 5,620,013, US 5,843,893 and US 5,627,162 disclose chemicals for destroying capsular cells.

The major limitation of the chemical methods disclosed above is the toxicity and adverse effects of chemicals on surrounding tissues.

International Patent Publication No. WO 01/54603, PCT / US01 / 03052, discloses systems and methods for treating cells at any point in the body, such as in the ocular lens coating. The system and method employ a position control device that positions the energy radiating device in relation to cells at any point in the body, such as cells of the energy radiating device and the lens capsular film. Thus, the energy radiated from the energy radiating device heats the cell to a temperature above body temperature to a temperature at which protein denaturation occurs in the cell, thereby killing the cell or preventing cell proliferation. The energy radiating device may comprise a container containing a heated fluid, which heats the cell to a desired temperature.

Disclosed herein is a method of heating cells to denature or coagulate and destroy.

International Patent Publication No. WO 98/18392, PCT / US96 / 17322, discloses a mechanism for destroying residual lens epithelial cells in the lens capsule of the eye. The instrument includes a probe and an insulating sleeve comprising an electrical energy source, an electrode electrically coupled to the electrical energy source, and including a distal end configured to be inserted into the eye between the eye's iris and the capsular coat. In this document the inventor discloses a method of electrically incinerating the capsular cells in order to kill the capsular cells.

The main limitation of the electrical method is that the delicate tissue around the cells can be burned together.

US Pat. No. 6,669,694 discloses medical instruments and techniques for highly localized thermally mediated therapies. The document describes a method of delivering high temperature energy to tissues in order to obtain the effect of removing cells.

US Patent No. 4,963, 142 discloses an apparatus for intraocular laser microsurgery.

Once a method and apparatus for performing intraocular laser microsurgery is disclosed, the apparatus includes a laser delivery system coupled to a probe capable of delivering laser energy through a suitable medium such as sapphire. The probe includes a coaxial canal for aspiration of removed tissue and / or fluid. The method includes removing tissue with a laser and aspirating the removed tissue and / or fluid, which method is useful for sclerostomy, vitrectomy, among other methods. It is useful as an alternative to phacomulsification. The document also describes a probe for performing intraocular laser microsurgery and removing molten tissue. The device disclosed in this document is for delivering laser energy, for melting tissue and for removing molten tissue.

The word 'melting' is a geological term that includes the meaning of melting by heat, or means to remove by dissolution or evaporation. The laser energy described herein is a means to achieve high energy levels sufficient to melt tissue and then to remove the molten or dissolved product. By using a laser, high energy can be achieved, and the laser can concentrate very high energy in a short time in a small area, and perform melting operation without damaging surrounding tissue.

US Pat. Nos. 6,238,386, 6,554,824, 6,582,421, 6,712,808 and 6,726,680 disclose devices for applying laser energy to human tissue.

US Pat. No. 6,454,762 discloses a device for applying light, in particular a laser beam, to a human or animal body. It also includes a movable tip to direct light energy or laser energy from an external light source to a desired portion of the human body.

US Pat. No. 6,238,386 discloses a method of applying sound energy and laser energy to an internal body cavity using an endoscope. The application to the inside of a human body by an optical fiber delivery system is disclosed. The laser used is a therapeutic laser and emits a laser having an optical power of at least 5 W (Watt) at the distal end or an intensity of at least 1 kW cm.sup.-2 at the distal end. The power is disclosed to be at the level required to solidify tissue.

The inventor discloses a device that uses laser energy and sound energy to treat the interior of the human body by an endoscope. However, the device uses energy to solidify tissue, as described above. The minimum energy disclosed in the invention is 5W. Since 1 W is 408 lux, the amount of energy used is 2040 lux / cm ² or 2040,0000 lux / m ² .

The device disclosed in this document uses very low energy up to 1000 lux / m ² from the cell side but at the same time uses higher energy from the basal membrane side. At this energy level no coagulation occurs. The device disclosed in this document directs energy towards the cell side and the basal membrane side, two specific directions simultaneously for the cell basal complex, in order to achieve the desired effect.

The laser has a high energy and can cause thermal solidification or thermal damage of the tissue by raising the temperature of the tissue to a high level with respect to the second fracture. However, the surrounding tissue may also melt when a high energy system such as a laser is used. If epithelial cells clot, this energy will damage the underlying coating. It is well known that the coating breaks at an energy level of 1.2 mJ. Therefore, the device of the present invention cannot be used in the ophthalmology field to separate epithelial cells from the capsule. This is because the cornea and the coating itself are damaged.

The prior art cited above is to solve the problem associated with destroying and removing epithelial cells on the encapsulation, and includes the following means as a general means.

A. Mechanical Means

These methods disclose a mechanical device for removing unwanted cells. The main limitation of these methods is that they can also damage surrounding tissues.

B. Chemical Means

These methods use chemicals to remove cells. The main limitation of these methods is that they can also be toxic to surrounding tissues.

C. Electrical Means

The main constraint is that soft tissues around the cells can also be incinerated.

D. Laser or sonic means / bright light source

The laser contains high energy and can result in thermal coagulation or thermal damage of the tissue by raising the temperature of the tissue to a high level with respect to the secondary fracture surface. However, when a high energy system such as a laser is used, the surrounding tissue may also melt. This damages the cornea and the coating itself.

The purpose of gently isolating cells from the basement membrane cannot be achieved via laser. This is because photocoagulated cells can adhere to the basement membrane and cause stronger adherence than before exposure to the laser.

The present invention provides a device for exposing the cell basement membrane complex to a particular low intensity light energy from one direction, ie from the cell side, and to expose higher intensity light energy from the basement membrane side, while simultaneously isolating these cells from the basement membrane. Implement

The device may include optical fiber tips or delivery mirrors. The delivery mirror may allow cells to be exposed to light energy simultaneously in two specific directions. The present invention overcomes various drawbacks of the prior art by providing methods for dissociating adherence between epithelial cells and the membrane by exposing epithelial cells to a light source and an apparatus for implementing a low intensity light source. The method of removing cells from the basement membrane may be performed by a simple washing method if desired.

This directly exposes the subject cells to a predetermined very low intensity light having a wavelength of 194 to 850 nanometers relative to the cell side, while at the same time exposing higher intensity light energy to the base membrane side by means of the device and method according to the invention. Is achieved. Low intensity light is directed from the cell side to the cells and not from the basement membrane side. Light is transmitted from the inside and actually touches the end of the light source carrier almost to the cell-film complex. Thus, the distance between epithelial cells and the light source is near zero. The exposure time is less than 60 seconds. The basement membrane side of the cell basement membrane complex is exposed to light energy having a predetermined specific wavelength, ie, a wavelength of 194 to 850 nm. The light may be interfering light or non-interfering light. The light irradiated to the cell basement membrane complexes specified herein has a wavelength of 194-850 nm and an illuminance of 0.002-50,000 lux.

If light comes from the normal outside, the cells are very resistant to this light, but if light is incident from the inside in the manner described herein, the cells are very sensitive to this light. The basal membrane side of the cell basement membrane complex should be exposed to higher intensity light energy of 0.002 to 500000 lux.

1 is a representative of a low intensity device for separating epithelial cells.

1: light source incident on the basement membrane side of the cell basement membrane complex

2: light source incident on the cell side of the cell basement membrane complex

3: basement membrane

4: cells

FIG. 2 is a diagram illustrating a device using a single light source, the filter and attenuator control the intensity and wavelength of light incident on the basement membrane complex from the basement membrane side and the cell side, which is very small on the cell side compared to exposure from the basement membrane side. Indicated to be exposed to intensity. At this time, the light is carried by the optical fiber cable.

1: single light source

5: fiber optic cable

6: Filters, attenuators and polarizers transport light energy to the base membrane side of the composite

7: Filters, attenuators and polarizers that carry weaker light with other specific spectral components on the cell side of the cell basement membrane complex

3 is a diagram showing that external light is directly irradiated to the basement membrane, but irradiated to the cell side through a reflecting mirror. Attenuators, filters and polarizers are schematically depicted and will be readily understood by those skilled in the art. The energy irradiated on the basal membrane side of the cell basement membrane complex is higher than the energy irradiated on the cell side of the cell basement membrane complex.

1: light source with wavelength of 194-1600 nm

9: filter / polarizer / attenuator

3: basement membrane

4: cells

10: filters / polarizers / attenuators

8: mirror

4 is a diagram illustrating that the base film is exposed to a light source incident from the outside. The light source passes through the transparent cornea and exposes the outside of the lens capsule to light energy. On the other hand, the optical fiber carries light from another light source or the same light source, but is modified by filters and attenuators, exposing the cell side of the composite to light energy.

11: external light source, such as surgical microscope light source

12: light irradiated to the basement membrane side of the tissue from an external light source

13: an optical fiber carrying light energy irradiated to another side of the cell basement membrane complex, that is, the cell side, from another light source or the same light source

14: The inner or cellular side of the cell basement membrane complex is exposed to light carried by an optical fiber with a soft atraumatic end

15: transparent cornea

16: Amputation of the capsular bag called the capsulerhexis opening

17: outside of the capsular bag

Figure 5 shows a smooth tip in contact with or close to the coating from the inside, showing the bent end and the dual light source device with the correct method of exposing and positioning the device tip.

19, 18: optical fiber light source

16: capsule or basement membrane

17: cells listed in the coating from the inside

6 shows two smooth bent rings. It consists of fiber strands or surrounds the fiber strands. Soft rings are atraumatic and do not damage other biological tissues in close proximity. The distance to the cell side of the cell basement membrane complex of the light carrier is very close.

20: Exposure to the basal membrane side of the cell basement membrane complex by a non-traumatically designed soft bent light delivery system

21: Basement membrane side exposed

22: Second light source exposed to the cell side of the cell basement membrane complex by another smooth surface non-traumatic conduit

23: Cell side of cell basement membrane complex

The invention will be described in detail with reference to FIGS. 1 to 6 above.

A device for incision of cellular basement membrane binding is a light source (1), a delivery system (5, 13) that delivers light to a specific point and, if the basement membrane or envelope is in the shape of a curved bag or envelope, to deliver energy to the capsular bag through the opening. , 17 and 18). The ends of the delivery systems 14, 20, and 22 where the device is in contact with the coating are soft and atraumatic.

In another embodiment, two light pipes transmit light to the eye, one of which is introduced into the capsular bag as shown at 18 and 19 of FIG. 5, and the other is from the outside. Investigate.

Light source

The light source may be interfering light or non-interfering light, and may be monochromatic light or polychromatic light. The light source may be a light emitting diode (LED) or a laser, arc lamp, tungsten filament light source or other light source. Sunlight can also be used as a light source.

The light source can be white or any other color. The white light source may be converted into a constant pure color light source using a filter. A single light source comprising filters can be used to generate a pure color wavelength, and the interior of the capsular bag can be exposed to pure colors. Mixed light sources of white light may also be used. The predetermined wavelength is 194 to 850 nm. Light intensity is an important part of the device. The intensity of the light source used in the present invention should be very low that the final incident light irradiated to the cells can produce an illuminance of 0.001 to about 1000 lux. A 40-watt household light bulb, if measured very close to the light bulb surface, produces an illuminance of thousands of lux.

In any of the preferred embodiments, the light source is switched on or off or pulsed several times per second.

There may be more than one light source such that cells are alternatively exposed to light of different wavelengths.

The second light source may be used in combination with the first light source. It may be an external light source of the surgical microscope or an entirely different light source. By the second light source, light is transmitted to the base film. Such a light source may be a light source by a small size light emitting diode, a filter, an optical focus lens, a solar light modified by a polarizer or attenuators, a laser light source or an external bulb.

In any of the preferred embodiments, such a light source is used with illuminance 0.002 to 500000 lux.

The second light source is essential and has a higher illuminance than the illuminance of the first light source acting from inside or from the cell basement membrane complex and should be irradiated to the basement membrane side of the cell basement membrane complex. The exposures must take place simultaneously to obtain the best effect. The second light source may be white or in other colors.

In order to satisfy the condition that the energy incident on the base membrane side is higher than the cell membrane side, one or more light sources are used to irradiate the base membrane of the cell base membrane complex.

If the first light source is white light and the filters are used to produce pure wavelengths so that they can be delivered into the coating, bypass the filters and add new filters and attenuators as shown in FIG. The first light source can also be used as the second light source.

Delivery system

Fiber optic cables (5 in FIG. 2, 13 in FIG. 4 and 18 and 19 in FIG. 5) or reflective mirrors (8 in FIG. 5) are used to transfer light energy directly to the cells.

The fiber optic cable may be sealed with a transparent waterproof tubular conduit to avoid contact with the tissue of the eye.

The ends of the conduits (14 in FIG. 4 and 20 and 22 in FIG. 6) are smooth and rounded so that the ends do not tear or damage the film even when the tip contacts the lower surface of the film.

Way

During the actual treatment, after the initial treatment, all debris and dirt adhering to the cell basement membrane complex are removed by gentle suctioning and washing. If this treatment is carried out on a dish or vessel in a laboratory, the liquid in which the cell basement membrane complex is stored is segregated from dirt or insoluble suspended particles. When this process is used in the body, such as in the case of cataract surgery, the nucleus of the cataract is removed. The cortex is also clean. Low intensity light is transmitted through the device to the capsular bag, and cells are exposed to the light from the inside. A microscope lamp can be used as the second light source for exposure from the base film side. Within the laboratory, the cell basement membrane complex is located on the slide and exposed to light sources from both sides, where the energy from the low intensity light source is directly irradiated on the cell side. In the laboratory, when the process is performed under a microscope, the lamp of the microscope can be used as a second light source, where the second light source simultaneously irradiates the base film with higher energy. From the device, cells are freed or separated by irradiating light of low intensity on the cell surface and high intensity light on the basement membrane surface. Isolated epithelial cells can be removed, if desired, by known methods such as simple washing and suction.

The device according to the invention can be effected by irradiating capsular cells with light from both sides simultaneously. One ray is irradiated to the front film from the outside. The light beam is derived from either a light source used for surgery or an external light source as a surgical microscope, and is transmitted to the front surface of the film by an optical pipe made of optical fiber. Light irradiated to the first half coating from the outside may have an illuminance of 0.002 to 500000 lux.

However, not only these external rays form the device, the device must necessarily include a particularly low illumination internal light that is simultaneously irradiated to cells from the inside at the same time.

Light from the light source used to process the cells from within the coating can be repeated on and off 1 to 15 times per second.

In another embodiment of the present invention, the light energy is transferred to the interior of the front coating by a series of mirrors located in the bent pipe, so that light is transferred through the hollow pipe on behalf of the optical fiber carrier and the reflecting mirror and prism They will turn to the path they need.

In another embodiment of the present invention, the light source is delivered to a point where the capsular cells can be directly exposed without carrying light through the optical cable using the reflective mirror shown in FIG.

However, the present invention is not limited to any particular application or environment. Rather, it will be apparent to one skilled in the art that the present invention may be advantageously applied to any application or environment using different low intensity light sources or a plurality of combinations thereof. It is also evident that it may be advantageously applied to methods of applying such low intensity light sources using any other direct or indirect method or means, such as a mirror or other reflecting device. It should be clearly understood that the description of the exemplary embodiments described below is for illustration purposes only and is not intended to be limiting.

Best embodiment

A. Device

Two light sources, a first light source comprising blue and red light emitting diodes, wherein the blue light is 360-420 nm and the red light is 700-850 nm. The light emitting diodes are turned on / off 0 to 15 times per second. It is used to irradiate cell basement membrane complexes from inside or from the cell surface. The light source is of low intensity, with roughness on the cell surface ranging from 0.001 to 1000 lux.

The second light source is light used directly from the surgical microscope. The light is used to irradiate the basal membrane side of the cell basal membrane complex directly through the cornea. To facilitate exposure, the pupil is mechanically enlarged by eye drops or a surgeon so that the iris deviates from the path of the second light source. The intensity of light used is determined so that the illuminance of the base film is 0.002 to 500000 lux.

Light from the first light source is collected by the optical fiber light pipe and transmitted to the inside of the eyeball. The distal end of the optical fiber is in the form of a cannula (20, 22 in FIG. 6), the end of which is transparent so that the light can be transmitted to the coating.

B. Preferred Examples-Methods

For the application of low-intensity devices to separate epithelial cells, the catheter is applied to the capsular bag with the nucleus and cortex removed, and a second light source from the surgical microscope can be used to enlarge the pupil medically or mechanically by the surgeon prior to surgery. Pull the iris to irradiate on the basement membrane. The coating is contacted from the inside in several places by the first conduit such that light from the device is instantaneously irradiated to various areas of the coating. The cells separate and float in the fluid in front of the eye. The isolated cells can be removed by known methods, such as by light washing and aspiration, by hand holding a syringe and conduit, or by an automated system via a phacoemulsification machine.

In the most preferred embodiment, the device according to the invention is different from the mechanical device disclosed in the prior art. The device according to the invention does not contain any movable parts, does not irradiate any high intensity light on the cell, and only light with a constant well defined wavelength and low intensity, for a well defined time, In particular for well defined portions of the cell basement membrane complex.

The device described in this application applies light energy to the cell side at a specific energy level that is thousands of times smaller than the energy used in the prior art. The energy transfer of the present invention is not intended to coagulate tissue, and the device disclosed in the above applications uses very little light energy for the cell side and higher light energy for the base membrane side of the cell basement membrane complex. The cells are gently separated or detached by cleaving the bond between the cells and the basement membrane so that they can be isolated.

Typical energies used in the prior art are disclosed as thousands of times the energy delivered as defined in the embodiments of the present invention. The device disclosed in this example uses roughness levels of 0.001 up to 1000 lux from the cell side and at the same time higher roughness levels of 0.002 to 500000 lux from the encapsulated or outer side. The energy required for the devices disclosed herein is used 0.0000024 W for illumination from the inside.

Claims (15)

It has a specific wavelength of 194 to 850 nm, and at the same time has a transport system that exposes the cell surface of the cell basement membrane complex and the basement membrane surface to two different levels of light energy, so that very low intensity energy is exposed to the cell side of the complex. One or more light sources; And The epithelial cells listed in the envelope or the basement membrane allow the light emitted from the device to be directly exposed without penetrating the basement membrane, and either of the light sources is directed from the device to the epithelial cell, if the energy does not penetrate the basement membrane. And an optical transport system for transporting the light energy to apply direct light energy. The method of claim 1, The light source, which is directly exposed to the cells, radiates a very small amount of light energy so that the final available energy acting on the capsular epithelial cells is 0.001 to 1000 lux, cutting off the bond between the cell and the basement membrane or membrane. Device. The method of claim 1, The light source acting on the cell side of the cell basement membrane complex originates from the outside, and the light is transmitted to the capsular cell by an optical fiber pipe passing light energy of 194 to 850 nm. Device for cutting the bonds between the coatings. The method of claim 1, The light source acting on the cell side of the cell basement membrane complex does not pass through the anterior or posterior membranes by using a reflecting mirror, and is directly transmitted to the point where the cells of the membrane can be exposed. Device to incision. The method of claim 1, If the basement membrane is in the form of an envelope or cells are listed inside the bag or envelope, the light energy is delivered to the inside or cell side of the cell basement membrane complex by a series of mirrors located in a bent pipe. And inverting the bond between the cell and the basement membrane or the membrane, wherein the light is redirected in the required path by the reflective mirrors and prisms while passing through the hollow pipe on behalf of an optical fiber carrier. The method of claim 1, And the light source itself is fitted to the cavity pipe, thereby directly exposing to the cell side of the inner cell basement membrane complex of the capsular bag. The method according to claim 1 to 6, An additional light source illuminates the capsular bag from the outside, such that the capsular bag is illuminated from both inside and outside at the same time, wherein the light source is externally interfering or non-interfering light, and reflects a wavelength of 194 to 850 nm and the basement membrane. Roughness is either monochromatic light or polychromatic light having 0.002 to 5,00,000 lux. The method of claim 7, wherein And said second light source being further added is a surgical microscope or other external light source. The method of claim 7, wherein If the basement membrane is bag or envelope shaped, the second light source is carried by an optical fiber light source and is irradiated directly to the front or outer surface of the basement membrane or coating. The method of claim 7, wherein And the second light source is transported to the outer surface of the basement film or the frontal film by using a reflecting mirror installed into the eyeball. The method of claim 7, wherein The second light source is directly transported to the front or outer surface of the base membrane located beyond the membrane and the capsule, the device for cutting the bond between the cell and the base membrane or the membrane, characterized in that directly illuminates the base membrane . The method according to claim 1, wherein If the basement membrane or envelope is in the shape of a sac or envelope, the device for cutting the bond between the cell and the basement membrane or membrane, characterized in that a portion of the device that enters the capsular bag is a circular tip. The method according to claim 1, wherein A device for entering the capsular bag and dissecting the bond between the cell and the basement membrane or membrane, characterized in that part of the device becomes a circular ring or sphere. 14. A method of using the device of any of claims 1 to 13, comprising the steps of: turning the end of the device into a base or film that is shaped like a bag or envelope; Directing the end of the device toward capsular cells; 15. A method as set forth in claims 1 to 13, comprising contacting the end of the device with close proximity to the cells and then washing or aspirating the cells from the basement membrane complex by a conventional wash suction system. How to use. 14. A method using the device as claimed in claims 1 to 13, wherein if the base membrane is in the shape of a sac or envelope, by positioning the second end on the outer or anterior surface of the base membrane or envelope, the first light source is A method using the device as described in claims 1 to 13, wherein the cells are irradiated from inside the sac and a second light source is irradiated from the outside to the basement membrane or the envelope.

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