KR20070065254A - Methods for detecting abnormal epithelial tissue - Google Patents

Abstract

The visibility of abnormal tissue under light having wavelength peaks which selectively identify abnormal tissue is enhanced in the presence of normal ambient light by viewing the tissue through lens which transmit the wavelength peaks but block transmission of other wavelengths.

Description

Method for detecting abnormal epithelial tissue {METHODS FOR DETECTING ABNORMAL EPITHELIAL TISSUE}

The present invention relates to a method for detecting aberrant epithelial tissue in which a tumor phenotype may be latent.

In another aspect, the present invention is directed to an improved method for real-time biopsy of epithelial tissue to detect abnormalities that can become cancerous cells or develop into invasive cancer cells.

It is evident that patients whose cancer counseling has been delayed for at least two months are more lethal than patients who are delayed shorter. Thus, if patients receive more effective cancer screening on a regular basis, their chances of dying from cancer will be reduced. Thus, there is a need for a simple and rapid screening test to detect aberrant mucosal tissue bearing a tumor phenotype and exhibiting the potential for development into invasive cancer cells.

Using selective light irradiation suitable for rapid and inexpensive screening, aberrant epithelial tissue can be visually identified and located in real time in vivo, in addition to routine irradiation of medical and dental surgery. For example, US Pat. Nos. 5,179,938 and 5,329,938 are machines equipped with chemiluminescent light sources that emit in the visible light, the green spectrum, the blue spectrum, and the red spectrum optionally, with spectral peaks of 450 nm and 550 nm. And 580 nm), which is incorporated herein by reference. Under illumination where normal ambient light is suppressed, the abnormal mucosal tissue appears white. For instance, such a selective optical device, for carrying out the in vivo tests are commercially available under the trademark of VIZILITE ^® Zila Pharmaceuticals, Inc. (Phoenix, Arizona, USA).

Selective viewing of aberrant mucosal tissues using the light source may be hindered by normal ambient light (natural or artificial) that pours into the tissue being examined, and standard procedures for such irradiation need to darken the laboratory. Shall be. Not only is this inconvenient, this may not be possible when the examination is performed in a room with large windows, or when another procedure is being performed by a conventional common light to another patient in the same room.

It is a primary object of the present invention to provide a method for such a selective light inspection that can be performed without darkening the laboratory. An optional light inspection method that can be performed where normal ambient light is present is described.

In short, the present invention for screening epithelial tissue for the possibility of abnormal tissue, illuminates the overall anatomical region of the epithelial tissue using light of a specified wavelength to help selectively visualize the abnormal tissue site on the overall region. illuminating) and preventing light of a wavelength other than the predetermined wavelength from being passed, while observing the total area through a filter lens through which the light of the predetermined wavelength passes, thereby detecting the abnormal tissue in the environment of ordinary ambient light. Improving the visualization of the site.

1 is a table illustrating spectral peaks of light provided in the present invention.

2 is a diagram illustrating light filtered by a lens provided in the present invention.

The following examples are provided so that those of ordinary skill in the art can understand and implement the present invention. The examples are provided for purposes of illustration, and do not represent the scope of the invention, which may be defined only by the appended claims.

Example  One

Routine oral visual examinations are conducted for any injuries to the gums, oral mucosa, bottom of the mouth, palate / study, and the back / side / back of the tongue. Injuries found by the routine inspection are recorded.

Example  2

After the routine examination of Example 1 was completed, the patient was instructed to rinse and spit the mouth with 1% acetic acid solution for 1 minute.

Chemiluminescent light sources, described in Lonky Patent US 5,329,938, which are activated by bending flexible outer capsules and breaking fragile inner vials, are commercially available under the trademark VIZILITE ^® . The capsule is then shaken and inserted into the retractor. The provided light has spectral peaks of about 450 nm and 550 nm and a smaller peak of about 600 nm in the red region (see FIG. 1). This spectral peak produces blue white light.

The clinician then examines wearing glasses with a lens that only passes light in the wavelength band of 400-600 nm (see FIG. 2). The glasses are designed to minimize illumination to the eyes of the examiner at the top and sides of the glasses. The glasses are commercially available under the registered trademark VIZILITE ^® of Zila Pharmaceuticals, Inc. (Phoenix, Arizona).

 Using the illumination provided by the light source, looking for injuries or suspected tissue areas that appear white, without reducing ambient light from a common illumination source, and any suspected tissue found in the routine inspection of Example 1 Visual attention to the oral cavity is repeated, paying particular attention to the site. Any site that appears white or blue-white is observed and recorded.

 Further assessment of the sites observed by histological sections of standard histology, or by molecular analysis, can determine whether the tissues are cancerous or contain mutations that are intermediate stages of invasive cancer development. .

Claims (10)

In the method of screening epithelial tissue for the possibility of abnormal tissue site, the method Illuminating the overall anatomical region of epithelial tissue using a light of a predetermined wavelength, wherein the light of the predetermined wavelength assists in selectively visualizing an abnormal tissue site on the overall area, and  While light of a wavelength other than the predetermined wavelength is prevented from being passed, the light of the predetermined wavelength is observed through the filter lens through which the entire region is observed, so that visualization of the abnormal tissue part in an environment where ordinary ambient light exists. Steps to improve Method for screening epithelial tissue in the possibility that there is an abnormal tissue site comprising a. In the method for detecting abnormal epithelial tissue, the method Illuminating an area of epithelial tissue using light having at least one specified wavelength, wherein the light is reflected from the area to form reflected light, Filtering the reflected light to remove wavelengths other than the one or more specified wavelengths, thereby forming filtered light, and Observing the filtered light Method for detecting abnormal epithelial tissue comprising a. The method of claim 2, further comprising detecting if the filtered light is white. 4. The method of claim 3, wherein if the filtered light is white, the method further comprises performing an evaluation of the area, wherein the evaluation is one of a histological section, histological analysis, or molecular analysis. Method for detecting aberrant epithelial tissue, characterized in that. 3. The method of claim 2, wherein said at least one specified wavelength is in the range of 400 nm to 600 nm. 3. The method of claim 2, wherein said abnormal epithelial tissue comprises tumor phenotypes. 3. The method of claim 2, wherein said light comprises ambient light, and wherein said ambient light is removed in said filtering step. 3. The method of claim 2, wherein said illuminating comprises directing light emitted from a chemiluminescent light source toward a region of said epithelial tissue. The method of claim 2, wherein the at least one wavelength comprises a first wavelength of 450 nm, a second wavelength of 550 nm, and a third wavelength of 600 nm. 3. The method of claim 2, further comprising providing glasses with a filter, wherein filtering the reflected light comprises filtering the reflected light using the glasses. Method for detection.

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