KR20070045174A - The natural immunity reinforcement proposal manufacturing method against the laying hen, chicken and hog - Google Patents

The natural immunity reinforcement proposal manufacturing method against the laying hen, chicken and hog Download PDF

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KR20070045174A
KR20070045174A KR1020070036284A KR20070036284A KR20070045174A KR 20070045174 A KR20070045174 A KR 20070045174A KR 1020070036284 A KR1020070036284 A KR 1020070036284A KR 20070036284 A KR20070036284 A KR 20070036284A KR 20070045174 A KR20070045174 A KR 20070045174A
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herbal
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이경호
배기화
김기용
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract

본 발명은 십전대보탕의 한방생약을 이용하여 가축의 면역력을 증가시켜 생산성을 향상시키고 아울러 고품질 무 항생제 한방브랜드 축산물을 생산할 수 있는 한방보조사료의 제조방법에 관한 것이다.

본 발명의 한방보조사료는 당귀 30 내지 40％, 작약 30 내지 40％, 천궁 10 내지 20％, 지황 10 내지 20％ 기타 십전대보탕 원료는 10％로 혼합하여 1 mm 이하로 분쇄 제조한다.

본 발명에서 제조된 한방보조사료는 적혈구의 증가, 백혈구의 감소를 통한 면역학적 생리 스트레스의 감소, 장내의 병원성 미생물의 생육억제등의 실험을 통하여 가축의 성장에 유의한 효과를 확인하였다. 따라서, 본 발명의 한방생약제로된 보조사료는 가축의 성장은 촉진시켜 항생제 미 사용시 나타나는 6％정도의 생산성 저하를 막아줄 뿐 아니라, 무 항생제 계란, 닭고기, 돼지고기를 생산하는데 이용할 수 있다.

[색인어]

허브믹스

Figure 112007503266991-PAT00010
, 증체량, 사료섭취량, 사료효율, IgG, 백혈구, 스트레스 지수

Figure 112007503266991-PAT00011

The present invention relates to a method for producing herbal supplements that can increase the immunity of livestock by using herbal medicines of Sipjeondaebotang to improve productivity and produce high quality non-antibiotic herbal brand livestock products.

Herbal supplements of the present invention is 30-40% Angelica, 30-40% Peony, 10-20% Cheongung, 10-20% Sichuan, etc. Other Siegejeonbotang raw materials are mixed in 10% and pulverized to 1 mm or less.

Herbal supplements prepared in the present invention confirmed a significant effect on the growth of livestock through experiments such as the increase of red blood cells, the reduction of immunological physiological stress through the reduction of white blood cells, the inhibition of the growth of pathogenic microorganisms in the intestine. Therefore, the supplementary feed of the herbal medicine of the present invention promotes the growth of livestock and prevents the productivity loss of about 6% when antibiotics are not used, as well as can be used to produce antibiotic-free eggs, chicken and pork.

[Index]

Herb Mix

Figure 112007503266991-PAT00010
, Weight gain, feed intake, feed efficiency, IgG, white blood cell, stress index

Figure 112007503266991-PAT00011

Description

산란계, 육계 및 양돈에 대한 천연면역증강제의 제조방법{The natural immunity reinforcement proposal manufacturing method against the laying hen, chicken and hog.}The natural immunity reinforcement proposal manufacturing method against the laying hen, chicken and hog.

도1은 양돈 및 육계 사료의 기본 배합표1 is a basic combination table of pig and broiler feed

도2는 허브믹스의 한방사료 기본 배합표Figure 2 is a herbal mix of herbal mix basic formulation table

도3은미생물 실험에 필요한 배지의 배합표3 is a combination table of the medium required for microbial experiments

도4은 1차 사양시험 결과4 is the first specification test result

도5는 2차 사양시험 결과5 is the secondary specification test results

도6은 1차처리사료의 영양소 소화율결과6 shows nutrient digestibility results of primary feeds

도7은 2차 처리사료의 영양소 소화율결과7 shows nutrient digestibility results of secondary feeds

도8은 1차혼합제제가 미생물 생육에 미치는 결과8 shows the results of the first mixture mixture on the growth of microorganisms

도9는 2차혼합제제가 미생물생육에 미치는 결과9 is the result of the secondary mixture agent on the growth of microorganisms

본 발명은 인체용 한의약 원료인 생약을 보조사료용으로 개발한 것이다. 좀 더 구체적으로, 본 발명은 십전대보탕의 주요성분들을 각 성분별로 가축에 급여하 여 유효한 성분을 집중강화시켜 만든 사료용 한방보조사료의 제조방법에 관한 것이다.The present invention is to develop a herbal medicine raw material for humans for auxiliary feed. More specifically, the present invention relates to a method of preparing herbal supplements for feed made by feeding the main components of the sipjeondaebotang to livestock by concentrating the effective ingredients.

일반적으로, 한의학에서는 개체별로 진맥을 짚어야 된다. 생약원료는 비싸서 접목이 어렵다. 두근은 열이 많아서 한방효과가 나타나기 어렵다는게 종래의 통설이였다.In general, in oriental medicine, the individual must make a diagnosis. Herbal medicine is expensive and difficult to graf. Traditionally, it was difficult to show the effects of oriental medicine because of the heat.

그러나 중앙대학교 동물자원과학과 연구팀과의 3년간의 연구 및 필드실험을 통하여 오히려 항생제를 대체할 만큼의 유의한 효과를 확인하였다.However, through three years of research and field experiments with the Department of Animal Resource Science, Chung-Ang University, it was found to be effective enough to replace antibiotics.

전통적 보약인 십전대보탕의 원료를 산란계, 육계, 돼지에 맞게 조성물의 비율을 달리하여 보조사료로 이용하면 종래 기술의 문제점인 면역성분의 증가, 항병력 강화를 통하여, 가축에게 한방생약이 유효하게 쓰일 수 있다는 것을 증명하고 본 발명을 완성하게 되었다.If the raw materials of the traditional medicine, Jeopjeondaebo-tang, are used as supplementary feeds with different composition ratios for laying hens, broilers, and pigs, herbal medicines can be effectively used for livestock through the increase of immune components and strengthening of anti-medical forces, which are problems of the prior art. It has been proved and the present invention has been completed.

결국, 본 발명의 목적은 십전대보탕의 주 생약제를 가축에게 맞게 조성물 비율을 달리하여 산란게, 육계, 양돈용 보조사료를 제조하는 방법을 제공하는 것이다.After all, an object of the present invention is to provide a method for producing a supplementary feed for laying crabs, broilers, and pigs by varying the composition ratio of the main herbal medicine of Sipjeondaebotang to livestock.

이와 같은 목적을 달성하기 위하여 본 발명은 인체에 있어 생리불순, 빈혈, 부인병 , 면역력 강화, 항균작용을 하는 지황, 당귀, 작약, 감초, 오미자, 천궁 등으로 이들 생약들을 적절히 배합하여 생산한 복합생약제제(Herb Mix

Figure 112007503266991-PAT00012
)로 이들 성분 중, 주요 성분 지황, 당귀, 천궁, 작약을 각각 30％ 강화하여 중추 산란계에서부터 투여 초기 산란 시기와 산란 피크 산란계의 생산성, 계란 품질 및 장내 미생물 균총 변화, 혈액성상 을 증가시키는 1단계, 상기 1단계에서 만들어진 제제로부터 효과가 좋은 두 개의 강화 성분을 혼합하여 새로운 배합비율의 New Herb Mx를 만드는 방법을 제공하는 2단계로 이루어 진다.In order to achieve the above object, the present invention is a complex herbal medicine produced by appropriately combining these herbal medicines such as dysmenorrhea, anemia, gynecological diseases, strengthening immunity, anti-bacterial action of sulfuric acid, donkey, peony, licorice, Schisandra chinensis, and uterus Herb Mix
Figure 112007503266991-PAT00012
Among these components, the main component, Rehmannia, Angelica, Cervix and Peony, are strengthened by 30% each, increasing the productivity, egg quality and intestinal microbial flora of the laying hen from the central hen, , By mixing two reinforcing ingredients with good efficacy from the formulations made in step 1 is made of two steps to provide a method of making a new formulation ratio of New Herb Mx.

이하 본 발명을 보다 자세하게 설명하면 다음과 같다.Hereinafter, the present invention will be described in more detail.

도1은 발명에 사용된 기본적인 사료 배합표이며, 도2는 허브믹스의 한방생약제제의 배합표이며, 도3은 발명에 사용된 미생물의 배합표이고, 도4는 1차 사양시험에 관한 결과이고, 도5는 1차사양시험의 결과를 확인하는 2차 사양시험에 관한 결과이고, 도6은 1차사양시험에 관해 영양소 소화율에 관한 결과이고, 도7은 1차 소화율시험에 관한 시험결과이고 도8은 1차 혼합제제가 미생물생육에 미치는 결과이고, 도9는 2차혼합제제가 미생물생육에 미치는 결과를 나타낸 표이다.Figure 1 is a basic feed formulation table used in the invention, Figure 2 is a formulation table of herbal medicines of herbal mix, Figure 3 is a combination table of the microorganisms used in the invention, Figure 4 is a result of the first specification test, Figure 5 is a result of the secondary specification test confirming the results of the primary specification test, Figure 6 is a result of the nutrient digestibility of the primary specification test, Figure 7 is a test result of the first digestibility test and Figure 8 Is the result of the primary mixed agent on the microbial growth, Figure 9 is a table showing the result of the secondary mixed agent on the microbial growth.

본 발명은 먼저, 첫번째 시험에 사용된 사료의 기본 배합표는 표1에 나타난 바와 같으며, 엔알씨(1998) 요구량에 준하여 작성되었고 이 사료를 대조구(티1)로 하여 허브믹스

Figure 112007503266991-PAT00013
0.2％ 첨가구(티2) 허브믹스
Figure 112007503266991-PAT00014
(지황 30％강화) 0.2％(티3) 허브믹스
Figure 112007503266991-PAT00015
(당귀 30％강화) 0.2％(티4) 허브믹스
Figure 112007503266991-PAT00016
(천궁 30％강화) 0.2％(티5) 허브믹스
Figure 112007503266991-PAT00017
(작약 30％강화) 0.2％(티6) 항생제 플라보마이신0.05％(티7) 사료를 만들었다. 1차 시험에서는 최초 사료를 중추 사료부터 시작하여 산란피크 사료까지 단계별로 ME가와 CP함량이 다른 사료를 단계별로 급이 하였다. 두번째 시험에서 사용된 사료는 대조구(T1)로 하여 허브믹스
Figure 112007503266991-PAT00018
0.2％ 첨가구(T2) 뉴허브믹스
Figure 112007503266991-PAT00019
0.1％ 첨가구(T3) 뉴 허브믹스
Figure 112007503266991-PAT00020
0.2％ 첨가구(T4) 뉴허브믹스
Figure 112007503266991-PAT00021
0.3％ 첨가구(T5), 항생제 아밀라마이신 0.05％(T6) 사료를 만들었다. 시험에 사용한 한방제제의 조성은 표2와 같다.In the present invention, first, the basic formulation table of the feed used in the first test is shown in Table 1, and was prepared according to the requirements of NRC (1998), and the herb mix was used as the control (T1).
Figure 112007503266991-PAT00013
0.2% Addition Sphere (Tea 2) Herb Mix
Figure 112007503266991-PAT00014
0.2% T3 Herb Mix
Figure 112007503266991-PAT00015
0.2% (Tea 4) Herbal Mix
Figure 112007503266991-PAT00016
0.2% (Tea 5) Herbal Mix
Figure 112007503266991-PAT00017
(Approximately 30% enhanced) A diet of 0.2% (T6) antibiotic flavomycin 0.05% (T7) was made. In the first trial, the initial feed was fed from the central feed to the spawning peak feed. The feed used in the second test was a control (T1) and mixed with herbs
Figure 112007503266991-PAT00018
0.2% addition port (T2) New Herb Mix
Figure 112007503266991-PAT00019
New Herb Mix with 0.1% Addition Port (T3)
Figure 112007503266991-PAT00020
0.2% addition port (T4) New Herb Mix
Figure 112007503266991-PAT00021
A 0.3% addition (T5) and antibiotic amylamycin 0.05% (T6) feed were made. The compositions of the herbal preparations used in the tests are shown in Table 2.

첫번째 시험동물은 14주령된 산란계(Hy-Line Brown) 1120수를 대조구를 포함하여 총 7개의 처리구로 구성하여 처리당 8반복, 반복당 10케이지, 케이지당 2수씩 수용하여 난괴법으로 배치하였다. 시험개시시시 개시체중은 개시체중 975±4이었다. 사양관리는 시험사료를 16주령부터 15주간 급여하고 시험기간 동안 물과 사료는 자유 섭취하게 하였고, 정상적인 점등 관리를 실시하였다. 두번째 시험은 45주령부터 51주령까지 총 6주간 720수를 대조구 포함하여 총 6개의 처리구로 구성하여 처리당 6반복 반복당 10케이지, 케이지당 2수씩 수용하여 난괴법으로 배치하였다. 산란율 10％ 도달 시 체중측정, 초란시기 및 산란피크 도달시기 비교, 산란율 50％부터 산란율(hen-day, hen housed), 평균 난중, 연파란율을 매일 측정하여 주 별 평균을 계산하였고, 2차 시험 또한 동일한 방법으로 측정 하였다.사료 섭취량은 주 1회 조사하여 사료 요구율을 산출하였다. 난각 품질 검사는 주 1회씩 주중 하루에 생산된 총 계란 중 연,파란을 제외한 계란을 취하여 실시하였다. 난각강도는 Texture Test Systems(T2100, Food Techology co., USA)를 이용해 측정하였고 난각색도와 색상은 Eggshell Color Fan를 이용하여 측정하였으며, Haugh unit는 Roush(1981)의 방법에 준하여 실시하였다. 난황 색도는 주 1회씩 주중 하루에 생산된 계란을 취하여 Egg yolk Color Fan(Roche)을 이용하여 측정하였다. 혈액 분석은 시험 종료일에 날개 정맥을 통해 1ml씩 반복당 5마리씩 처리당 40수(1차시험)/30수(2차시험)를 채혈한 후 EDTA 처리된 튜브에 담아 냉장 보관 12시간 이내에 HEMACYTE™(OSI Oxford Science Inc, 2003, USA)를 이용하여 혈액 분석(Leukocytes; WBC, NE, LY, Erythroctes; RBC, Hb, HCT)을 실시하였으며, NE값과 LY값을 이용하여 stress indicator를 측정하였다. 시험 종료 시 대조구와 시험구에서 각각 3수씩 임의적으로 선별하여 총 42수(1차)/36수 (2차) 를 가지고 소하장부를 각각 10ml씩 일정하게 절개하여 모든 내용물을 멸균된 용기에 담아 분석 전까지 -75℃에서 냉동보관 하였다. 냉동보관한 장내용물을 약 1g을 멸균된 15㎖ test tube에 담고 멸균된 증류수 9㎖을 첨가하여 희석 (10-1)시킨 후 10-2～10-8까지 단계적으로 희석하였다. 세종류의 선택 배지 평판에 희석된 샘플을 1㎖씩 접종시키고 혐기적 (GasPack System, BBL Microbiology System, Becton Dickinson ＆ Co., Cockeysville, MD 2130, USA) 또는 호기적으로 배양하였다. 선택배지 및 배양 조건은 Table 3에 나타난 바와 같다. 배양 후 미생물의 수를 각 평판의 colony-forming unit (CFU)로 계산 후 log10으로 환산하였다. 시험에서 얻어진 자료의 통계처리를 위하여 각 반복당 주당 평균생산성을 SAS

Figure 112007503266991-PAT00022
(1995)의 GLM(General Linear Model) Procedure를 통해 산란생산성, 계란 품질 및 장내 미생물 수를 분석하였으며, 처리의 평균간 비교는 Dun-can's new multiple range test에 의하여 P ＜ 0.05에서 검정하였다.The first test animal was composed of 7 treatment groups, including a control group, 1,120-year-old Hy-Line Brown 1120, containing 8 repetitions per treatment, 10 cages per repetition, and 2 per cage. The onset weight at the start of the test was 975 ± 4. Specimen management was allowed to feed the test feed for 16 weeks to 16 weeks, free water and feed during the test period, and normal lighting management was performed. The second test consisted of a total of six treatments, including a control group, for a total of 6 weeks from 45 to 51 weeks of age, including 10 cages per 6 repetitions per treatment and 2 cages per cage. When the 10% egg production rate is reached, the weight average, the egg production period and the peak time of egg production are compared, and the egg production rate (hen-day, hen housed), average egg weight and annual egg yolk rate are measured every day from 50% egg production rate, and the second average is calculated. The test was also measured in the same way. Feed intake was examined once a week to yield feed requirements. The egg shell quality test was conducted once a week by taking eggs except soft and blue eggs. Eggshell strength was measured using Texture Test Systems (T2100, Food Technology Co., USA). Eggshell color and color were measured using Eggshell Color Fan. Haugh unit was carried out according to Roush (1981). Egg yolk color was measured using Egg yolk Color Fan (Roche) once a week produced eggs per week. At the end of the test, blood samples were taken from the wing vein at 40 ml (1st trial) / 30 (2nd trial) per treatment, 5 ml per repetition, and then placed in EDTA-treated tubes within 12 hours of refrigeration for storage with HEMACYTE ™ (OSI). Blood analysis (Leukocytes; WBC, NE, LY, Erythroctes; RBC, Hb, HCT) was performed using Oxford Science Inc, 2003, USA). Stress indicators were measured using NE and LY values. At the end of the test, the control and test groups were randomly selected by 3 numbers each, and total 42 counts (1st) and 36 counts (2nds) were taken, and 10 ml of the intestine was constantly cut out, and all contents were placed in a sterile container for analysis. Frozen storage at -75 ℃ until. About 1 g of the stored intestinal contents were placed in a sterile 15 ml test tube, and diluted (10 −1 ) with 9 ml of sterilized distilled water, followed by dilution stepwise from 10 −2 to 10 −8 . Diluted samples were inoculated in 1 ml of three selective media plates and cultured anaerobicly (GasPack System, BBL Microbiology System, Becton Dickinson & Co., Cockeysville, MD 2130, USA) or aerobic. Selection medium and culture conditions are shown in Table 3. After incubation, the number of microorganisms was calculated by colony-forming unit (CFU) of each plate and converted into log 10 . The average productivity per week for each iteration was SAS for statistical processing of data obtained from the test.
Figure 112007503266991-PAT00022
(1995) analyzed the egg production, egg quality, and intestinal microbial count through the General Linear Model (GLM) procedure, and the comparison between treatments was tested at P <0.05 by Dun-can's new multiple range test.

이상에서 상세히 설명하고 입증한바와 같이, 본 발명은 한방생약 보조사료의 제조방법을 제공한다. 본 발명에서 제조된 한방생약 보조사료는 닭, 돼지이 장내유 해 미생물의 생육을 억제하고, 백혁구의 감소 면역물질인 IgG의 감소 등을 통하여 닭에서는 증체육 향상, 산란율 증가 및 계란품질의 향상, 돼지에서는 증체율 향상, 항생제 대체 효과를 확인하였다.As described and demonstrated in detail above, the present invention provides a method of manufacturing herbal herbal supplements. Herbal herbal supplements prepared in the present invention inhibit the growth of microorganisms in the intestinal harmful microorganisms of chickens and pigs, and in the chicken through the reduction of IgG, immunoreactive substances of Baek Hyukgu, the increase in weight gain, egg production rate and egg quality, pigs Confirmed the effect of weight gain and antibiotic replacement.

따라서 본 발명의 한방생약 보조사료는 양계, 양돈사료에서 문제가 되고 있는 면역저하성 생산성 저하 문제를 예방, 해결하는데 이용될 수 있다.Therefore, the herbal herbal feed of the present invention can be used to prevent and solve the problem of lowering the productivity of immunosuppression, which is a problem in poultry and pig feed.

Claims (1)

당귀 30 내지 40％, 직약 30 내지 40％, 천궁 10내지 20％, 지황 10내지 20％ 기타 십전대보탕원료 10％를 첨가하는 생산제조방법Production method of adding Angelica 30-40%, Occupation 30-40%, Cheongung 10-20%, Turmeric 10-20%, and other 10% of Jeopjeondaebotang raw materials
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101016393B1 (en) * 2010-09-20 2011-02-18 (주) 오도그네트웍스 Veterinary sipjeondaebotang multivitamin containing ingredients and manufacturing methods
CN103918938A (en) * 2014-04-29 2014-07-16 严斯文 Method for processing laying hen feed
CN104304723A (en) * 2014-09-19 2015-01-28 合肥市好旺养殖科技有限公司 Feed formula for breeding boars
CN106107198A (en) * 2016-07-01 2016-11-16 瓮安县乌江蛋鸡养殖有限公司 A kind of functional egg feedstuff and preparation method thereof
CN107080102A (en) * 2017-06-29 2017-08-22 寻甸志英养殖有限公司 The formula forage and its cultural method of a kind of laying hen

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101016393B1 (en) * 2010-09-20 2011-02-18 (주) 오도그네트웍스 Veterinary sipjeondaebotang multivitamin containing ingredients and manufacturing methods
CN103918938A (en) * 2014-04-29 2014-07-16 严斯文 Method for processing laying hen feed
CN104304723A (en) * 2014-09-19 2015-01-28 合肥市好旺养殖科技有限公司 Feed formula for breeding boars
CN106107198A (en) * 2016-07-01 2016-11-16 瓮安县乌江蛋鸡养殖有限公司 A kind of functional egg feedstuff and preparation method thereof
CN107080102A (en) * 2017-06-29 2017-08-22 寻甸志英养殖有限公司 The formula forage and its cultural method of a kind of laying hen

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