KR20070043902A - Increasing production of recombinant protein by growth hormone - Google Patents

Abstract

The present invention relates to a method for increasing the amount of recombinant protein produced by growth hormone. Specifically, the present invention will be useful for increasing production by treating growth hormone to CHO cell lines transformed with foreign genes.

Recombinant protein, growth hormone

Description

Increasing production of recombinant protein by growth hormone

1 is a pIRES-DHFR / IGFBP-3 vector map,

2 is pIRES-DHFR / IGFBP-3 vector for CHO (dhfr -) of the picture showing the molecular characteristics of the transformed cell lines recombinant animal cells (CHO-YSBP3), A is a recombinant animal cell line CHO-YSBP3 genomic PCR is performed using DNA as a template, B is RT-PCR performed using RNA of CHO-YSBP3 template, C is Western blot performed with CHO-YSBP3 culture medium,

Figure 3 is a CHO-YSBP3 cell line incubated with α-MEM and CHO-S-SFM-II culture medium, A is a picture performed ELISA method, B is a picture performed Western blot,

Figure 4 is a picture of measuring the protein increase and the gene copy number change after treatment with growth hormone to recombinant cell line CHO-YSBP3 by Western blot and PCR method,

5 shows a comparison of purified protein amount according to growth hormone throughput.

The present invention relates to a method for increasing the production of recombinant protein by growth hormone, and more particularly, to a method for increasing the production of recombinant protein by treating and culturing growth hormone in a CHO cell line transformed with a foreign gene.

Currently, a variety of expression systems, such as microorganisms, plants, yeasts, insect cells, animal cells, etc. are used to express and obtain a large amount of a target protein and to use it for treatment. The most easily used expression system is a microbial expression system and various microbial expression systems have been developed and commercialized. However, microbial expression systems have some limitations. The biggest limiting factor is the microbial protein expression and protein modification mechanisms (glycosylation, phosphorylation, amidation), and unlike the animal cells, even if the same gene is expressed in the microbial system, the structure or characteristics of the expressed protein are different. It is not exactly the same as the protein expressed in. Because of this, the recombinant protein using the microbial expression system is currently limited to proteins that rarely undergo protein modification or that have no functional difference even when the protein is modified or changed in structure. In addition, in using a recombinant protein using a microbial expression system, it is cumbersome that the secondary contaminant removal process must be accompanied due to microbial contamination, microbial endotoxin contamination, and the like.

In contrast, although animal cell expression systems are the most suitable systems for expressing animal proteins, they are not easily industrialized due to their low expression efficiency, high production cost, and difficulty in manipulating animal cells compared to microbial expression systems. Currently used animal cells include Chinese Hamster Ovary (CHO), Baby Hamster Kidney (BHK), Myeloma (Myeloma), etc., and the expression vector is introduced into the cell line in the same manner as the microbial expression system to express the foreign protein of interest. . Among these cell lines, dhfr -deficient CHO cell lines are the most widely used animal cell lines for industrial mass production of target proteins using animal cells. CHO cell lines lacking dhfr are firstly similar to humans in posttranslational modification of proteins, ie, glycosylation and phosphorylation, secondly in suspension culture as well as in adherent culture, and thirdly in serum-free medium. It is possible to cultivate a relatively high concentration compared to other cells, and fourthly, to increase the productivity of the target protein, which is significantly lower than that of microorganisms, using dihydrofolate reductase (DHFR) and MTX (methotrexate) gene amplification system. Fifthly, the stability is verified and it is easy to obtain permission from a supervisory authority such as FDA. By solving the modification process of the animal protein using the recombinant animal expression system using this, it is possible to omit the complicated refolding process of the expression protein, thereby preventing time loss. In animal expression systems, there has been a report on the production of recombinant target protein by transforming an anti-stage gene into a CHO cell line (Korean Patent No. 454016).

Growth factors are polypeptides that cause a wide range of biological responses in specific cell populations, such as DNA synthesis, cell division, expression of specific genomes, and the like. Many different growth factor groups include metastatic growth factor beta (TGF-β), epidermal growth factor (EGF), metastatic growth factor alpha (TGF-α), platelet-derived growth factor (PDGF), fibroblast growth factor Group (FGF) and insulin-like growth factor group (IGF), including insulin-like growth factor-I (IGF-I) and insulin-like growth factor-II (IGF-II).

IGF-I and IGF-II are peptides that promote the growth of many cells and act by autocrine and paracrine methods. These mitogenic effects are associated with IGF-I receptors on the cell surface. By binding to and activating tyrosine kinase (Japes et al ., Experimental Cell Research , 116: 336-340 1988; King et al . , J Clin Invest , 66: 130-140 1980). IGF-I and IGF-II are related to each other in amino acid sequence and structure, and each polypeptide has a molecular weight of approximately 7.5 kDa. IGF-Ⅰ is involved in the main effects of growth hormone and is an important substance involved in postnatal growth. In addition, IGF-I production is increased when cells are treated with various other growth factors, indicating that they are involved in the action of various growth factors. In contrast, IGF-II plays a major role in fetal growth. Both IGF-I and IGF-II have insulin-like activity and promote cell division of cells of nerve tissue, muscle, regenerative tissue, skeletal tissue and various other tissues. Unlike most growth factors, there is a substantial amount of IGF in the circulation, and only trace amounts of IGF are free in the circulation or in other body fluids. Most of the circulating IGF is associated with IGFBP-3. IGFBP-3 binds with IGF with high affinity to transport IGF, increase half-life and play a regulatory role in binding free IGF to IGF receptors (Rajaram et al. al ., Endocr Rev , 18: 801-831, 1997). Therefore, IGFBP-3 mostly suppresses the stimulatory effect of IGF-I on cell proliferation, but in some cases, IGFBP may be enhanced. IGF-I is measured in serum to diagnose abnormal growth related diseases such as pituitary giantism, acromegaly, microcertification and various growth hormone deficiencies. Although IGF-I is produced in many tissues, most of the circulating IGF-I is synthesized in the liver. Almost all IGF-I or IGF-II circulate in ternary complexes that are non-covalently bound to IGFBP-3 and larger protein subunits called acid labile subunits (hereinafter referred to as "ALS"). ALS has no direct IGF-binding activity and only binds to the IGF-I / IGFBP-3 binary complex. Ternary complexes of IGF-I / GFBP-3 / ALS have a molecular weight of approximately 150 kDa. The ternary complex acts as a reservoir and buffer for IGF-I and IGF-II, which prevents abrupt changes in free IGF concentrations in the circulation (Blum et al. al . , In Modern Concepts of Insulin-like Growth Factors, p. 381-393, EMS Pencers, ed., Elseviver, New York, 1991).

Mostly circulating IGF-I, IGF-II and IGFBP-3 are in the form of complexes and therefore there is little detectable free IGF. Moreover, the presence of high concentrations of free IGF in the blood causes severe hypoglycemia due to the insulin-like activity of IGF. In contrast to IGF and IGFBP-3, the free ALS pool is in plasma so that all IGF-I / IGFBP-3 complexes entering the circulation immediately form ternary complexes.

While IGFBP-3 is the most common IGF binding protein in the circulation, at least six distinct IGF binding proteins have been identified in various tissues and body fluids. Although the proteins bind to IGF, each contains an amino acid sequence that is distinct from a separate gene, so the binding proteins are not analogs or derivatives of simple common precursors.

IGF-I and IGFBP-3 can be purified from natural sources or prepared by recombinant methods. For example, there is a method for purifying IGF-I from human serum (Rinderknecht et. al , PNAS , 73: 2365-2369 1976). A method for preparing IGF-I by recombinant methods is known from European Patent No. 0,128,733, published December 1984. IGFBP-2 can be purified from natural sources using methods such as Baxter et al . (Baxter et. al ., Biochem Biophys Res Comm , 139: 1259-1261, 1986). Growth hormone dependent insulin-like growth factor (IGF) binding proteins obtained from human serum are distinguished from other human IGF binding proteins (Baxter et. al ., Biochem Biophys Res Comm , 139: 1259-1261, 1986. IGFBP-3 can be synthesized by recombinant organisms as discussed by Tressel et al . (Tressel et. al ., Biochem Biophys Res Comm , 178 (2): 625-331, 1991). Recently, a case of purifying 0.24 mg IGFBP-3 per liter in MEL-C88 cell line using LCR / Mel expression system has been reported (Bagnall et. al ., Prot . Express . Purific . , 27: 1-11, 2003)

Growth hormone secreted by the pituitary gland stimulates the growth of all possible tissues in the body. In addition, growth hormones are known to have an effect on metabolic processes such as substantially increasing the rate of protein synthesis in all cells, decreasing the rate of carbohydrate metabolism in cells, mobilizing free fatty acids and increasing the use of fatty acids for energy. .

The present inventors completed the present invention by confirming that the production of recombinant human IGFBP-3 is increased by treating growth hormone to CHO cell lines expressing recombinant human IGFBP-3.

An object of the present invention is to provide a method for increasing the production of recombinant protein comprising the step of adding a growth hormone to the culture of cells transformed with a foreign gene.

In order to achieve the above object, a growth hormone is added to the culture of a CHO cell line transformed with a foreign gene to provide a method for increasing the yield of recombinant protein.

Hereinafter, the present invention will be described in detail.

The present invention provides a method for increasing recombinant protein production of a cell line transformed with a foreign gene by adding growth hormone to a culture. In a preferred embodiment of the present invention, IGFBP-3 is used as a recombinant protein, but the IRES vector, which is a vector used in the present invention, does not use a special inducible transcriptional regulator such as a CMV promoter as a transcriptional regulator. It can be seen by those skilled in the art that the foreign protein mass production method of the present invention does not depend on the expression vector or the type of foreign gene used. Thus, the method of the present invention is not limited to the method for mass production of IGFBP-3, and can be used for mass production of other foreign proteins. Cell line used in the present invention dhfr Examples were performed using gene-deficient CHO cell lines, but the present invention is not limited thereto.

CHO cell line lacking the dhfr gene used in the present invention is a cell line that does not express the DHFR protein involved in the synthesis of hypoxanthine and thymidine, which are essential components of the cell, and is a commercially available cell line. (ATCC: CRL 9096) It may also be prepared directly using conventional transformation methods known to those skilled in the art.

The present inventors selected IGFBP-3 as a recombinant protein to prepare a CHO cell line lacking dhfr transformed with a foreign recombinant gene, but the present invention is not limited thereto. In this regard, the present inventors constructed a recombinant IGFBP-3 expression vector. As a vector system for expressing two conventional genes, there are a form in which a plurality of promoters are inserted, and a combination of one promoter and an IRES series (Internal Ribosomal Entry Site). A vector having a plurality of promoters causes a problem that only the efficiency of expression from either promoter is high due to interference between the promoters. The IRES vector system is a system in which two genes are expressed in one expression vector, and the protein at the 5 'site of the promoter is known to have a 5-10-fold higher expression rate than the protein at the 3' site. The animal cell expression vector used in the present invention is pIRES (Clontech. Inc., USA) having an internal ribosome entry position. Dhfr on the 3 'side to increase the gene copy number of recombinant IGFBP-3 in the same gene copy number in cells A gene was inserted and a recombinant expression vector (pIRES-DHFR / IGFBP-3) was prepared by inserting the recombinant IGFBP-3 gene into the 5 'side (see FIG. 1). The expression vector pIRES-DHFR / IGFBP-3 prepared as described above was transformed into CHO cells, and was primarily selected using G418, a selection marker in the recombinant vector, by limiting some amino acids in the culture medium ( dhfr ). Only cells with were secondary selected. In order to increase intracellular copy number of intracellular dhfr gene, the concentration of rhIGFBP-3 secreted into the culture medium was measured by increasing the concentration of MTX (methotrexate) and using techniques such as genomic DNA PCR, RT-PCR and Western blot. The cell line that secretes the most recombinant protein was selected and named as the recombinant cell line CHO-YSBP3.As of October 10, 2005, it was deposited with the accession number KCTC10860BP to the Korea Institute of Bioscience and Biotechnology, 52 Ueo-dong, Yuseong-gu, Daejeon, Korea. (See Figure 2).

 The recombinant cell line CHO-YSBP3 obtained above was cultured in a serum-free medium and grown in a condition of α-MEM containing serum, α-MEM containing no serum or serum-free CHO cell line culture medium CHO-S-SFMII. Hormones were treated at concentrations of 0, 0.1, 1, 100 and 1,000 ng / ml and incubated for 24 and 48 hours to determine the yield and gene copy of recombinant IGFBP-3 by Western blot and PCR methods (see FIG. 4). As a result, the production of recombinant IGFBP-3 increased with growth hormone treatment under all conditions. Among them, the serum-free medium conditions significantly increased the production of recombinant IGFBP-3 in CHO-S-SFMII culture.

The recombinant cell line CHO-YSBP3 obtained above was incubated with α-MEM and CHO-S-SFMII cultures for 48 hours in serum-free medium, and the recombinant IGFBP-3 was treated with insulin-like growth factor I (IGF-). Ⅰ) Purification by affinity chromatography method showed that α-MEM and CHO-S-SFMII cultures were treated with CHO-S-SFMII cultures when treated with 1 ng / ml of growth hormone 3.2 times more than α-MEM. Production was increased, and when cultured with the same CHO-S-SFMII culture, growth hormone treatment resulted in a 1.5-fold increase in production (see Table 1).

Hereinafter, the present invention will be described in more detail with reference to Examples.

However, the following examples are only for exemplifying the present invention, and the contents of the present invention are not limited to the following examples.

< Example  1> IGFBP -3 Expression Vector Construction

To prepare an IGFBP-3 expression vector, a polymerase was prepared using primers set forth in SEQ ID NO: 1 or 2 to insert Xba I and Sal I cleavage sites using human IGFBP-3 cDNA (Gene Bank accession No. NM000598) as a template. Amplification was performed by a polymerase chain reaction (hereinafter referred to as "PCR"). PCR was performed for 5 minutes at 94 ° C. as the initial denaturation, followed by 25 times at 30 ° C. at 30 ° C., 30 seconds at 60 ° C., and 30 seconds at 72 ° C. for a total of 25 times. PCR products were then electrophoresed using 1% agarose gel and purified on gel using a QIAquick kit (Qiagen, USA). The purified product was digested with Xba I and Sal I. The product was amplified by PCR using primers set forth in SEQ ID NOs: 3 and 4 to insert Nhe I and Sal I cleavage sites. PCR products were then electrophoresed using 1% agarose gel and purified on gel using a QIAquick kit (Qiagen, USA). The purified product was cut into Nhe I and Sal I. The product was inserted into the Xba I - Sal I cleavage site of the pIRES (Clontech. Inc, USA) vector, and the dhfr gene was inserted into the vector. PCR was performed using the primers set forth in SEQ ID NO: 7 or 8 using Human dhfr cDNA (Gene bank accession No. NM000791) as a template. PCR was performed for 5 minutes at 94 ° C. as the initial denaturation, followed by 25 times at 30 ° C. at 30 ° C., 30 seconds at 60 ° C., and 30 seconds at 72 ° C. for a total of 25 times. The prepared vector was named pIRES-DHFR / IGFBP-3 (see FIG. 1).

< Example  2> animal cells week ( CHO , dhfr -) Transformation

Animal cell lines CHO (dhfr -, Chinese Hamster Ovary ) (ATCC: CRL9096) were converted the animal cell transfected with the expression vector pIRES-DHFR / IGFBP-3 produced in Example 1. 8 ml / DMEMF12 culture medium (10 mg / L hypopoxanthine, 10 mg / L, thymidine 10% Fetal bovine serum) (Gibco-BRL, USA) with CHO cells in a 100 mm ² culture plate with a cell count of 1 × 10 ⁶ cells / plate. The mixture was incubated for 18 hours at 37 ° C., 5% CO ₂ , 95% air and 100% humidity incubator. Subsequently, 5 μg of recombinant animal cell expression vector pIRES-DHFR / IGFBP-3 was diluted in 700 μl of serum-free DMEM / F12 culture medium (10 mg / Lhypoxanthine, 10 mg / L thymidine) (Gibco-BRL, USA) and Lipofectamine ^™. (Gibco-BRL, USA) 20 μl of serum-free DMEM / F12 culture medium (10 mg / Lhypoxanthine, 10 mg / L thymidine) (Gibco-BRL, USA) was diluted to 20 μl of the vector dilution 20 μl of Lipofectamine dilution After mixing with the mixture was allowed to stand at room temperature for 15 minutes. The cultured CHO cell culture plates were washed twice with PBS buffer and the Lipofectamine ^™ mixture was mixed with 5 ml of serum-free DMEM / F12 culture (10 mg / lhypoxanthine, 10 mg / l thymidine) (Gibco-BRL, USA). Cell culture plates were added and incubated for 5 hours in a mixed incubator at 37 ° C., 5% CO ₂ , 95% air and 100% humidity. The culture was then replaced with 10 ml of DMEM / F12 culture (10 mg / l hypoxanthine, 10 mg / l thymidine, 10% FBS) (Gibco-BRL, USA) and 37 ° C., 5% CO ₂ , 95% air and 100% humidity. Incubated for 48 hours in a mixed incubator. After 48 h, CHO cells were isolated from the plate using trypsin-EDTA solution, and 100 mm ² DMEM / F12 culture medium (10 mg / lhypoxanthine, 10 mg / l thymidine, 10% FBS, 550 μg / ml G418) with the selection marker G418 solution added to the culture plate at 1 × 10 ⁵ cells / plate (Gibco- BRL, USA) and incubated to induce death of cells without a selection marker. Primary cells were selected in DMEM / F12 culture (10 mg / l hypopoxanthine, 10 mg / l thymidine, 10% FBS, 200 μg / ml G418) in a 96 well plate with 1 × 10 ⁴ cells / plate. BRL, USA), diluted 200 μl per well and incubated in a mixing incubator at 37 ° C., 5% CO ₂ , 95% air, and 100% humidity, when one clone was full, 0.25% trypsin-EDTA The cells were recovered by treatment with the solution, and then aliquoted into each well of a 24 well plate to secure 30 clones. Cultures of 30 selected clones were stored and cultured with Hep3B (ATCC: HB-8064) secreting IGFBP-3 as a positive control using an anti-hIGFBP-3 antibody (Santa Curz Biotechnology, Inc, USA). Western blot was performed to confirm the expression level. Western blot was loaded onto the SDS-PAGE gel and the supernatant recovered from Hep3B transferred to the nitrocellulose membrane, and the primary antibody, anti-IGFBP-3 (goat polyclonal IgG) (Santa Cluz Biotechnology, Inc., USA) After binding to anti-goat IgG-HRP (anti-goat IgG-HRP) (Santa Cluz Biotechnology, Inc., USA) was combined to confirm the expression of IGFBP-3.

Example 3 Transformation Cell Line CHO Clone Selection

Among clones selected in Example 2, clones with high expression rates were selected to amplify gene copies with MTX (methotrexate). The clones were confirmed to be transformed into α-MEM culture (10% FBS, 200 μg / ml G418) (Gibco-BRL, USA) in order of 0.008, 0.02, 0.04, 0.08, 0.32, 0.64, 1 μM As the concentration was increased, the cells were incubated for one week at each concentration to increase the number of copies of the recombinant gene in the cells. Culture conditions are the same as those of Example 2. The protein was secreted for each MTX concentration, and the protein expression level was measured by Western blot using the anti-hIGFBP-3 antibody in the same manner as in Example 2. In addition, genomic DNA and total RNA can be obtained from Menitas et al. (Menitas et. al ., Molecular Cloning, 3rd Ed, Cold Spring Harber Laboratory Press, 2001). The isolated genomic DNA was used as a template and amplified by PCR using primers described in SEQ ID NOs: 5 and 6. PCR was performed for 5 minutes at 94 ° C. as the initial denaturation process, and then a total of 25 times were performed for 30 seconds at 94 ° C., 30 seconds at 60 ° C., and 30 seconds at 72 ° C. After 25 repetitions, the reaction was performed at 72 ° C. for 5 minutes. The PCR product was then electrophoresed using 1.5% agarose gel.

As a result, it was confirmed that the IGFBP-3 gene was inserted into each genomic DNA, and the copy number was confirmed to be different for each clone (see FIG. 2A). RT-PCR also confirmed that RNA expression occurs normally (see FIG. 2B). Western blot using anti-IGFBP-3 antibody confirmed the highest expression of clone # 3 at the MTX concentration, and clone # 3 was obtained. Clone # 3 was thus named CHO-YSBP3 and was deposited on October 10, 2005 at the Korea Research Institute of Bioscience and Biotechnology, 52 Ueo-dong, Yuseong-gu, Daejeon, Korea (Accession No .: KCTC10860BP).

< Example  4> Selected clone CHO - YSBP3 Cultivation conditions

In order to purify the recombinant protein secreted into the culture medium, a culture medium that minimizes the amount of the protein present in the selection medium I (α-MEM, 10% FBS, 200 μg / ml G418, 0.08 μM MTX) (Gibco-BRL, USA) was prepared. Used. Recombinant animal cell line CHO-YSBP3 was dispensed in 6-well plates at 2 × 10 ⁵ cells / plate and incubated for 24 hours in a mixed incubator at 37 ° C., 5% CO ₂ , 95% air and 100% humidity in a conventional selective culture I. Cells were then washed with PBS and washed at 37 ° C., 5% CO ₂ , 95% air and 100% humidity in Selected Culture II (α-MEM, 200 μg / ml G418, 0.08 μM MTX) (Gibco-BRL, USA). The cultures were incubated for 24, 48 and 72 hours in a mixed incubator. In addition, the culture solution was incubated with a difference of 10, 5, 2.5, 1 and 0.1% concentration of FBS. After 24 hours in selective culture I, washed with PBS and cultured in CHO-S-SFM-II culture medium (0.08 μM MTX) (Gibco-BRL, USA) to culture CHO cell lines at 37 ° C., 5% CO ₂ , 95 Incubation was carried out for 24, 48 and 72 hours in a mixed incubator of% air and 100% humidity and the culture was recovered. In addition, the culture solution was incubated with a difference of 10, 5, 2.5, 1 and 0.1% concentration of FBS. Using the recovered culture as a sample, the ELISA plate coated with the anti-IGFBP-3 antibody was quantified by ELISA technique, and Western blot was performed in the same manner as in Example 2 to confirm the expression of IGFBP-3. . The ELISA technique was as follows. After IGF-1 was coated on the plate, a complex was prepared under various conditions, and the prepared solution was injected into the plate. After reacting at 37 ° C. for 1 hour, only IGFBP-3, which did not form a complex, bound to the coated IGF-1 on the plate, and the complexed IGF-1 / IGFBP-3 was washed. In this state, an anti-BP-3 polyclonal antibody (Santa Cluz Biotechnology, Inc., USA) is injected, washed, and then a secondary antibody, anti-mouse-IgG-HRP (Santa Cluz Biotechnology, Inc., USA) Was added, and TMB, the substrate, was added to induce color development and measured at 450 nm.

As a result, in 48 hours of culture in CHO-S-SFM-II culture, the cell line was smaller than that of 24 hours, but the production time was determined as 48 hours by confirming that IGFBP-3 production was superior to the number of cells (Fig. 3).

< Example  5> Recombination by Growth Hormone IGFBP -3 yield increase survey

Recombinant cell line CHO-YSBP3 was incubated in serum-free medium for 24 hours, followed by selective culture I (α-MEM, 10% FBS, 0.08 μM MTX, antibiotics 1X) (Gibco-BRL, USA) and conventional serum-free culture II (α). -MEM, 0.08μM MTX, antibiotics 1X) (Gibco-BRL, USA), commercially available cultures (CHO-S-SFMII, 0.08μM MTX, antibiotics 1X) (Gibco-BRL, USA) The growth hormone was treated at concentrations of 0, 0.1, 1, 10, 100 and 1,000 ng / ml to recover the culture at 24 and 48 hour intervals, and recombined using Western blot and PCR techniques as in Example 3. The protein amount and gene copy number of human IGFBP-3 were compared relatively. At this time, PCR was performed using primers set forth in SEQ ID NO: 1 or 2 to insert Xba I and Sal I cleavage sites using human IGFBP-3 cDNA (Gene Bank accession No. NM000598) as a template. PCR was performed for 5 minutes at 94 ° C. as the initial denaturation, followed by 25 times at 30 ° C. at 30 ° C., 30 seconds at 60 ° C., and 30 seconds at 72 ° C. for a total of 25 times. The PCR product was then electrophoresed using 1.5% agarose gel.

As a result, it was confirmed that the expression of the growth hormone was increased in all culture conditions than in the non-treatment. In addition, the production of recombinant human IGFBP-3 was increased under the culture conditions of CHO-S-SFMII (see FIG. 4A). As the concentration of growth hormone increased, the number of copies of the gene also increased (see FIG. 4B).

< Example  6> growth hormone treated CHO - YSBP3 And Purification of Recombinant IGFBP-3 from Cultured Culture Samples

<6-1> Recombination IGF -1 affinity column ( CNBr  4B IGF -One affinity column Production

To prepare an IGF-1 affinity column, CNBr-activated Sepharose 4B powder (Amersham Pharmacia Biotech, USA) was dissolved in 1 mM HCl to neutralize the gel. The recombinant IGF-1 protein expressed and purified under the culture conditions established in Example 5 was dissolved in binding solution (0.1M NaHCO ₃ , 0.5mM NaCl, pH8.3) and used as a ligand to the CNBr-4B gel. After binding for 16 hours at 4 ° C., the column was washed with a 5-fold volume of binding solution and left at 4 ° C. for 2 hours with the addition of 0.1M Tris-HCl solution (pH 8.0) to remove the remaining active groups. The column was then prepared by washing the column in the order of 0.1M Tris-HCl solution (pH 8.0), 0.5M NaCl with 0.1M acetic acid (pH 4.0) and 0.1M Tris-HCl solution (pH 8.0).

<6-2> recombination IGF -1 affinity Column  Recombination IGFBP -3 Separation and Purification

Incubate the recombinant cell line CHO-YSBP3 into 1 × 10 ⁷ cells / plate in a 150 mm ² culture plate under the culture conditions established in Example 5 and dilute in Selected Culture I to 37 ° C., 5% CO ₂ , 95% air and 100 Incubate for 24 hours in a mixed incubator at% humidity, wash twice with PBS, replace with CHO-S-SFM-II culture (0.08 μM MTX) (Gibco-BRL, USA) and incubate for 48 hours to recover the culture. It was. At this time, the culture medium was treated with 1 ng / ml of growth hormone and CHO-S-SFM-II cultures with 0.1 and 1 ng / ml of growth hormone. The collected culture solution was removed from the cell suspension through a 0.22 μm ultrafiltration filter, and the CNBr-4B-IGF-1 affinity column prepared in Example 6-1 was added at 1 ml / min, and 0.5M acetic acid (pH 3.0). Was added at 1ml / min and fractionated by 10ml. The second of the fractions was substituted with acetic acid by PBS via dialysis in PBS pH 7.4 and concentrated using a Viva-spin 10 kDa protein concentrator (25 mL, Satorius) (see FIG. 5). The results are shown in the table below.

α-MEM + GH (1 ng / ml) CHO CHO + GH (0.1ng / ml) CHO + GH (1 ng / ml) Protein (mg) 0.24 0.56 0.84 0.77 Cell number Fold One 1.1 1.1 Fold One 2.3 3.5 3.2 Fold One 1.5 1.4

As a result, when the growth hormone was cultured in the CHO-YSBP3 cell line in the present invention, the production of recombinant human IGFBP-3 was 1.5-fold higher than that of the conventional method, and the protein production was greater than the increase in cell number.

As described above, the present invention is a method for increasing the production of recombinant protein by growth hormone, and increases the production of recombinant IGFBP-3 by culturing the growth hormone in the CHO-YSBP3 cell line, 1.5 times more than the conventional method In view of the above, it can be usefully used to produce a hypoglycemic agent or a hepatoprotective agent.

<110> Riz biotech <120> Increasing production of recombinant protein by growth hormone <130> 5p-08-27 <160> 8 <170> KopatentIn 1.71 <210> 1 <211> 20 <212> DNA <213> human IGFBP-3 XbaI primer <400> 1 gctctagaat gcagcgggcg 20 <210> 2 <211> 22 <212> DNA <213> human IGFBP-3 SalI primer <400> 2 tgcggtcgac ctacttgctc tg 22 <210> 3 <211> 23 <212> DNA <213> PCR product hIGFBP-3 NheI primer <400> 3 cggctagcat ggttcgacca ttg 23 <210> 4 <211> 21 <212> DNA <213> PCR product hIGFBP-3 DalI primer <400> 4 cggtcgactt agtctttctt c 21 <210> 5 <211> 20 <212> DNA <213> rhIGFBP-3 5 'primer <400> 5 gctctgcgtc aacgctagtg 20 <210> 6 <211> 20 <212> DNA <213> rhIGFBP-3 3'primer <400> 6 gcttcctgcc tttggaaggg 20 <210> 7 <211> 20 <212> DNA 213 dhfr sense primer <400> 7 ctcccaaagt gctgggatta 20 <210> 8 <211> 20 <212> DNA 213 dhfr antisense primer <400> 8 cttgaaccaa agggttgcat 20

Claims (5)

A method for increasing production of recombinant protein comprising adding growth hormone to a culture of animal cells transformed with a foreign gene. The method of claim 1, wherein the foreign gene encodes IGFBP-3 (insulin growth factor bindign protein-3). The method of claim 1, wherein the animal cell is a CHO-YSBP3 cell line deposited with accession number KCTC10860BP. The method of claim 1, wherein the growth hormone is treated at a concentration of 0.1 ng / ml to 1 ng / ml. 1) culturing the recombinant cell line in serum culture for 24 hours; 2) replacing the medium with serum-free medium (CHO-S-SFMII), treating growth hormone, and incubating for 24 hours. Method for increasing the yield of recombinant protein comprising a.

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