KR20070025637A - Microbeam device for irradiating individual cell and method for irradiating individual cell using the same - Google Patents

Abstract

A microbeam device for radiating an individual cell and a method for radiating an individual cell using the same are provided to selectively radiate a radiation ray as a cell unit with a low energy transmission power. A microbeam device for radiating an individual cell includes a beam generating part(102), a beam transport box(302), a microbeam generating part(402) and a cell radiation frame(516). The beam generating part generates beams. The beam transport box is connected to the beam generating part at its one surface, and transmits the beam generated from the beam generating part, and has a vacuum window formed at the other surface. The microbeam generating part is disposed at the other surface of the beam transport box, and generates microbeam from the beam. The cell radiation frame includes a cell vessel and a cell vessel mounting part disposed on the microbeam generating part opposite to the beam transport box.

Description

Microbeam device for irradiating individual cells and method for irradiating individual cells using same {Microbeam device for irradiating individual cell and method for irradiating individual cell using the same}

1 is a cross-sectional view showing a preferred embodiment of the micro-beam device for irradiation of individual cells according to the present invention.

Figure 2 is an enlarged view of the beam transport box and the micro beam generating unit constituting a preferred embodiment of the micro-beam apparatus for irradiating individual cells according to the present invention.

3 is an enlarged exploded view of the vacuum window and the micro beam generating unit of the beam transport box constituting a preferred embodiment of the micro-beam apparatus for irradiating individual cells according to the present invention.

4 is a cross-sectional view of a cell container constituting a preferred embodiment of the microbeam device for individual cell irradiation according to the present invention.

5 is a cross-sectional view of a cell vessel mount that constitutes a preferred embodiment of the microbeam device for individual cell irradiation according to the present invention.

6 is a conceptual diagram illustrating the real-time monitoring of the microbeams irradiated to the cells using the microchannel plate of the method according to the present invention.

FIG. 7 confirms the effect of the surrounding magnetic field shielding of the preferred embodiment of the microbeam device for irradiating individual cells according to the present invention by simulation using a MATLAB PDE toolbox.

FIG. 8 shows energy spectra measured using a PIPS detector for a 70 keV electron beam in a preferred embodiment of the individual cell irradiation microbeam device according to the present invention compared with simulation results using an EGS code. .

Figure 9 shows the fluctuation characteristics of the number of electrons incident on a cell in a state in which a preferred embodiment of the individual cell irradiation microbeam device according to the present invention is operated in the pulse mode.

FIG. 10 shows the spatial distribution of the electron beam intensity passing through the microbeam generating unit, which constitutes a preferred embodiment of the microbeam device for individual cell irradiation according to the present invention.

FIG. 11 is a simulation of total cell dose distribution around cells evaluated using EGS codes for electron beams of 30, 50 and 70 keV, respectively, in order from the top in the preferred embodiment of the individual cell irradiation microbeam device according to the present invention. The results are shown.

Explanation of symbols on the main parts of the drawings

102: beam generator 104: cathode assembly

106: HV floating supply box 108: turbomolecular pump

110: auxiliary pump 112: gate valve

202a and 202b: anti-vibration tables 204a and 204b: beam generator support

206: X / Y deflector 208: Focus lens

302: beam transport box 304: gate valve

306: primary collimator 308: Faraday Cup

310: turbo molecular pump 312: auxiliary pump

402: micro-beam generator 404: microchannel plate

406: microchannel plate mounting portion 408: Mylar thin film

410a, 410b, 410c, 410d: mylar thin film holding and vacuum holding o-ring

412a, 412b: Mylar thin film fixing ring 414: pinhole frame

416: pinhole 502: cell container mounting portion

504: cell container 506: cell container frame

508: Mylar retaining ring 510: Mylar thin film bottom

512: scanner connection 514: motor driven scanning stand

516: cell probe vs. 602: microscope

604: CCD camera 606: image acquisition

702: system control and image processing unit 704: motor control unit

706: beam generation unit control unit 708: HV power supply unit

710: pulse generator

The present invention relates to a micro-beam device for individual cell irradiation capable of dynamic control and individual cell irradiation method using the same.

Recent breakthroughs in molecular biology have led to the completion of human genome maps, research into the genetic interpretation of the genome, and the structural and functional roles of genomes / proteins, and attempts to observe and interpret cellular responsiveness to radiation from a molecular biology perspective. ought. From the molecular biology perspective, with the attempt to interpret the DNA damage caused by oxidative stress and its natural repair process in the metabolism of normal cells, the difference between DNA damage caused by radiation and DNA damage caused by oxidative stress, An attempt has been made to interpret the natural recovery potential of DNA damage.

The possibility of DNA damage by the radiation is directly related to the amount of radiation energy delivered to the DNA and surrounding materials. Increasing or decreasing radiation levels at high doses can be explained by linear increases and decreases in absorbed dose of cells. However, the energy delivered to cells and small molecules at low radiation levels, i.e., low dose radiation exposure, is not uniform across all cells, and increasing or decreasing the level does not necessarily mean increasing or decreasing the absorbed dose of each cell.

Microbeam cell irradiation apparatus is used in the above research process. In other words, the microbeam cell irradiation apparatus forms a beam corresponding to the size of the cell or cell nucleus to irradiate the cells to precisely define whether and where the cells are exposed and to observe the biological effects caused by the radiation exposure based on the radiation. It is facility to do.

Conventional microbeam cell irradiation technology uses a high-density proton, alpha-particles and heavy-charged particles, such as charged particle beams with high LET or Linear Energy Transfer, or x-rays for easy beam direction control in the medium. It is limited to the microbeam irradiation apparatus to be used. On the other hand, micro-electron beam apparatus for individual cell irradiation has not been developed until now to induce an electron beam having complex and irregular beam propagation characteristics to a target cell to induce a desired radiation dose.

In order to construct such a micro-beam device for individual cell irradiation, (1) the influence of the surrounding magnetic field on the beam propagation in the vacuum, (2) the beam propagation in the atmosphere and the reaction between the passing medium, (3) the beam On the basis of the information on the secondary radiation generation characteristics of the quantitative evaluation technique of the beam direction change and the beam intensity and energy loss in a specific environment, and the beam transmission characteristics of the fabricated device and the Nuclear metrology techniques are needed to verify the functionality of the device by characterization, but so far It is not developed.

Therefore, the present inventors have continued research to solve the above problems, and as a result, it is possible to control the direction of the radiation beam, in particular the electron beam, and preserve the intensity of the micro beam in the medium, as well as the beam exposure amount of individual cells. The present invention was completed by developing a micro-beam apparatus for irradiating individual cells that can be monitored and effectively used for radiation exposure studies.

It is therefore an object of the present invention to provide a microbeam device for individual cell irradiation capable of dynamic control.

Another object of the present invention is to provide a method for irradiating individual cells using the microbeam device for irradiating individual cells.

In order to achieve the object of the present invention, the present invention is a beam generating unit for generating a beam, one side is connected to the beam generating unit and passes through the beam generated from the beam generating unit, the other side includes a vacuum window A beam container, a micro beam generator disposed on the other side of the beam container and generating a micro beam from the beam, and a cell container and a cell container mount disposed on a micro beam generator opposite the beam transport box. It includes a cell irradiation table, the inner wall of the beam transport box is provided with a micro-metal device for individual cell irradiation, characterized in that it is lined with mumetal.

In the micro-beam apparatus for irradiating individual cells according to the present invention, the beam may be selected from the group consisting of an electron beam, a proton beam, an α-ray beam, and a heavy charged particle beam.

In the micro-beam device for irradiation of individual cells according to the present invention, the beam is preferably an electron beam.

The micro-beam apparatus for irradiating individual cells according to the present invention includes a cell position control unit for adjusting a relative position of a cell container with respect to a micro beam, a cell image acquisition unit including a fluorescence microscope, a processing unit of the obtained image, and the beam generation. The controller may further include at least one of a negative controller and a vacuum pump connected to the beam generator and the beam transport box.

In the micro-beam apparatus for individual cell irradiation according to the present invention, the mumetal is preferably lined to a thickness of 0.01 ~ 0.5 inches.

In the micro-beam apparatus for individual cell irradiation according to the present invention, it is preferable that the upper end of the vacuum window is flattened, and a Mylar thin film is fixed to the upper end of the vacuum window.

The mylar thin film is preferably fixed using an O-ring and a retaining ring.

In the microbeam device for individual cell irradiation according to the present invention, the bottom of the cell container is preferably composed of a Mylar thin film.

In the micro-beam apparatus for individual cell irradiation according to the present invention, it is preferable to further include a microchannel plate disposed below the bottom of the vacuum window.

In order to achieve another object of the present invention, the present invention comprises the steps of generating a beam by the beam generator of the micro-beam apparatus for irradiating individual cells according to the present invention; Passing the generated beam through a beam transport box and generating a micro beam by a micro beam generator; And irradiating the generated micro-beams to cells in the cell container.

In the method of the present invention, monitoring average microbeam characteristics before and after irradiation using a passivated implanted planar silicon (PIPS) detector, or real-time monitoring of the microbeam irradiated to the cells using a microchannel plate. It is preferred to further comprise a step.

Hereinafter, the present invention will be described in more detail.

The inventors of the present invention, in the development of the micro-beam device for irradiation of individual cells according to the present invention, to solve the problems of the beam transmission and the formation of beam intensity due to the use of the difficult to control the beam, such as the electron beam as follows. The design concept was established:

(1) Since the electrons are small in mass and easily bent by the surrounding magnetic field such as the earth's magnetic field, even in vacuum, the electrons deviate greatly from the initial traveling direction when they reach the final micro beam former. Thus, there is a need for techniques to minimize propagation path changes of beams, in particular electron beams, within beam transport boxes;

(2) The beams that pass through the microbeam former, in particular the electron beams, are exposed to the atmosphere, causing severe scattering that can lead to the loss of the beam in the other direction or severe energy loss before reaching the cell target. have. Therefore, there is a need for a technique in which the loss of beam intensity and energy is kept to a minimum until it exits the vacuum window and reaches the target cell; And

(3) Since the beam, in particular the electron beam, is difficult to maintain an initial path of progression in the atmosphere outside the vacuum window, the number of electrons incident on the target cell is measured in real time using a method such as a cell irradiation apparatus using a high dose of charged particle beam. You cannot monitor it. Therefore, a new design concept for real-time monitoring of beams is required.

Hereinafter, a preferred embodiment of a microbeam device for individual cell irradiation according to the present invention and an individual cell irradiation method using the same will be described in detail with reference to the accompanying drawings.

One aspect of the invention relates to a micro-beam device for individual cell irradiation capable of dynamic control.

1 is a cross-sectional view showing a preferred embodiment of the micro-beam device for irradiation of individual cells according to the present invention.

Referring to FIG. 1, the micro-beam apparatus for irradiating individual cells according to the present invention includes a beam generator 102 generating a beam, and one surface thereof is connected to the beam generator 102 to be generated from the beam generator 102. And the beam passes through, and the other side is a beam transport box 302 including a vacuum window, the micro beam generator 402 is disposed on the other surface of the beam transport box 302 to generate a micro beam from the beam, And a cell irradiation table 516 including a cell container 504 and a cell container mounting part 502 disposed on the micro beam generator 402 opposite the beam transport box 302.

In the micro-beam apparatus for irradiating individual cells according to the present invention, the beam may be selected from the group consisting of an electron beam, a proton beam, an α-ray beam, and a heavy charged particle beam.

In the micro-beam device for irradiation of individual cells according to the present invention, the beam is preferably an electron beam.

Referring back to FIG. 1, the beam generator 102 includes a cathode assembly 104. In addition, the beam generator 102 is preferably connected to the turbo molecular pump 108 and the auxiliary pump 110 for the vacuumization thereof. The capacity of the turbomolecular pump 108 may be, for example, 30 L / sec. The degree of vacuum in the beam generator 102 may be, for example, 3.0 × 10 ⁻⁸ torr.

Preferably, the gate valve 112 is positioned between the beam generator 102 and the turbomolecular pump 108. In addition, the beam generator 102 is connected to the HV floating supply box 106, the HV floating supply box 106 is connected to the beam generator control unit 706 under its control, the HV power supply unit ( 708 is preferably connected. The beam generator controller may be connected to the pulse generator 710. The beam generator 102 may be operated in a pulse mode using the pulse generator 710. The beam generator controller 706 may be controlled by the system control and image processor 702.

The beam generator 102 may be supported by the beam generator supporters 204a and 204b, and is preferably connected to the anti-vibration tables 202a and 202b.

The beam generated by the beam generator 102 passes through the beam transport box 302. The beam transport box 302 has one surface connected to the beam generator 102 to pass through the beam generated and propagated from the beam generator 102, and the other surface includes a vacuum window.

The beam transport box 302 is preferably vacuum to prevent problems such as scattering of the beam by the medium. The beam transport box 302 is preferably connected to a turbomolecular pump 310 and an auxiliary pump 312 for the evacuation. The capacity of the turbomolecular pump 310 may be, for example, 70 L / sec. The degree of vacuum in the beam transport box 302 may be, for example, 5.0 × 10 ⁻⁸ torr.

2 is an enlarged view of the beam transport box 302 and the micro beam generator 402 constituting a preferred embodiment of the micro beam device for individual cell irradiation according to the present invention.

2, one surface of the beam transport box 302 may be configured as a gate valve 304, and the other surface may include a micro beam generator 402. The beam transport box 302 may include a primary collimator 306, a Faraday cup 308. The first collimator 306 serves to primarily reduce the cross-sectional area of the beam, and its diameter is not particularly limited, but is preferably 2 mm, for example. The Faraday cup 308 can be used to monitor the beam in the beam transport box 302.

Referring back to FIG. 2, in a preferred embodiment of the individual cell irradiation microbeam device according to the present invention, the inner wall of the beam transport box 302 is preferably lined with a mumetal 314. The mumetal 314 is preferably lined to a thickness of 0.01 ~ 0.5 inches. If the thickness of the lining mumetal 314 is 0.01 or less, the shielding effect of the external magnetic field is not sufficient, and if the thickness of the lining mumetal 314 is 0.5 or more, a problem of occupying too much space of the beam transport box 302 may occur.

In order to confirm the effect of the mumetal 314, the 70 keV electron beam emitted from the electron gun of the individual cell irradiation microbeam device without the mumetal is bent while running at 25 cm to the front end of the beam transport window As a result of measuring the degree, it was about 8 mm (result not shown). When aiming at the magnetic field attenuation coefficient of 100, it was confirmed that a thickness of about 10 inches is required when the permeability of the shielding material is 100, and about 0.1 inches when the permeability is 10,000. The mumetal was found to have a permeability of about 80,000 and required a thickness of 0.01 inches.

7 shows the effect of the surrounding magnetic field shielding of the preferred embodiment of the microbeam device for individual cell irradiation according to the present invention by simulation using a MATLAB PDE toolbox. 7, since the mumetal 314 invests and absorbs most of the surrounding magnetic field, it can be seen that the inside of the mumetal 314 becomes an area free from the surrounding magnetic field. Lining the mumetal 314 to 0.02 inches can maintain an internal magnetic field at a very low level of 10 mG or less.

Referring again to FIGS. 1 and 2, the microbeam device for individual cell irradiation according to the present invention preferably further comprises a microchannel plate 404 disposed below the bottom of the vacuum window of the beam transport box 302. . The microchannel plate 404 may be fixedly disposed below the bottom of the vacuum window, but it is preferable that the microchannel plate 404 is disposed to be detachably fixed. According to the microchannel plate 404, it is possible to monitor in real time whether the cells are exposed to the beam.

Referring again to FIGS. 1 and 2, the micro beam apparatus for irradiating individual cells according to the present invention includes a micro beam generator 402 disposed on the other side of the beam transport box 302 and generating a micro beam from the beam. Include.

3 is an enlarged exploded view of the vacuum window and the micro beam generator 402 of the beam transport box 302 constituting a preferred embodiment of the micro-beam apparatus for individual cell irradiation according to the present invention.

Referring to FIG. 3, the micro beam generator 402 may be configured of a pinhole frame 414 having a pinhole 416 and a hole having a predetermined diameter. Although the diameter of the said hole is not specifically limited, For example, 5 micrometers is preferable for irradiation control of a cell unit. The beam is converted into a micro beam having a simple size of micro size while passing through the micro beam generator 402, and then irradiated to the cell.

Referring back to FIG. 3, it is preferable that the upper end of the vacuum window is flattened for intimate contact with the cell container, the pinhole frame is mounted at the center of the upper end, and the mylar thin film 408 is fixed at the upper part of the pinhole frame. Do. The thickness of the mylar thin film 408 is not particularly limited, but is preferably 0.0005 to 0.003 mm.

Fixing the mylar thin film may be performed using O-rings 410a, 410b, 410c and 410d and fixing rings 412a and 412b.

In the case of using the Mylar thin film 408 as described above, the strength and energy loss of the micro beam can be kept to a minimum until the vacuum window exits to reach the target cell.

Referring again to FIG. 1, the individual cell irradiation microbeam device according to the present invention includes a cell container 504 and a cell container mounting part 502 disposed on the micro beam generator 402 opposite the beam transport box 302. And a cell irradiation table 516 including).

4 is a cross-sectional view of a cell container 504 that constitutes a preferred embodiment of a microbeam device for individual cell irradiation in accordance with the present invention.

Referring to FIG. 4, the cell container 504 fixes a cell container frame 506, a cell container bottom 510 connected to the cell container frame, and the cell container bottom 510 to a cell container frame 506. It is preferable that the mylar fixing ring 508 is configured. The cell container bottom 510 is preferably made of Mylar thin film.

When the cell container bottom 510 is made of Mylar thin film as described above, it has the advantage that the intensity and energy loss of the micro beam can be kept to a minimum until it exits the vacuum window and reaches the target cell. The mylar thin film has an appropriate tensile strength and can properly function as a cell container.

5 is a cross-sectional view of a cell vessel mount 502 that constitutes a preferred embodiment of a microbeam device for individual cell irradiation in accordance with the present invention.

Referring to FIG. 5, the cell container mount 502 may include a hole having a shape corresponding to the cell container 504, and an edge of the hole may protrude. The cell vessel mount 502 is preferably connected to the cell position control via the scanner connection 512. The cell position control unit includes a scanner connection unit 512, a motor driven scanning stage 514 connected to the scanner connection portion 512, a cell irradiation stage 516 disposed below, and the motor driven scanning stage 514. It may include a motor control unit 704 to adjust. The motor controller 704 may be controlled by the system control and image processor 702.

The relative position of the cell container 504 with respect to the micro beam can be easily adjusted by the cell position control unit.

Referring back to Figure 1, it is preferable that the micro-beam apparatus for individual cell irradiation according to the present invention includes a cell image acquisition unit and a processing unit of the image obtained above. The cell image acquisition unit may include a fluorescence microscope 602 and a CCD camera 604 connected to the fluorescence microscope 602 on the cell container. The obtained image 606 may be transmitted to the system control and image processor 702 for processing.

Another aspect of the present invention relates to a method for irradiating cells using the microbeam device for irradiating individual cells.

Individual cell irradiation method according to the invention comprises the steps of generating a beam by the beam generator of the micro-beam device for individual cell irradiation according to the invention; Passing the generated beam through a beam transport box and generating a micro beam by a micro beam generator; And irradiating the generated microbeams to cells in a cell container.

Preferably, the individual cell irradiation method according to the present invention is designed to confirm the stable beam characteristics by using a PIPS (passivated implanted planar silicon) detector, and to monitor the micro beam irradiated to the cells in real time using a microchannel plate You can include additional concepts.

Conventional devices using proton, helium ion and heavy ion particle beams facilitate the real-time monitoring of the beam because the beam's transmission power is high and the beam's straightness is maintained even after passing through the intermediate medium. For example, in the case of Columbia University, a gas-filled ion mounted on a cell culture vessel, as the protons and helium ion beams produced by the 4-MV van de Graf accelerator lose a very small amount of energy as they travel through the target cell and go straight ahead. Permeate particles are detected in real time. In the case of the Texas A & M University device, an 8 μm thick plastic scintillator is inserted into a vacuum in front of the cell culture vessel to measure the beam current in real time, and the beam transmitted through the scintillator is irradiated to the cell.

In the case of using such a radiation as well as the highly scattered beam as in the device according to the present invention, placing the detector in front of the target cell results in rapid degradation of the quality of the primary beam, for example, energy loss and severe changes in the direction of travel. When it occurs, the beam is difficult to deliver to the target cell, and if it is placed behind, the straightness of the beam is also lost while passing through the target cell and the culture solution.

To solve this, the apparatus and method according to the invention use a PIPS detector or microchannel plate.

6 is a conceptual diagram illustrating the real-time monitoring of the microbeams irradiated to the cells using the microchannel plate of the method according to the present invention.

Referring to FIG. 6, the microchannel plate 404 is used to monitor the primary beam incident on the cell in real time through measurement of secondary electrons emitted from the pinhole material during cell irradiation. In other words, by inserting the microchannel plate 404 in front of the vacuum window, the secondary electrons emitted backward by the primary beam that is not filtered through the pinhole material but passes through the pinhole among the beams traveling to the vacuum window are indirectly detected. It is the method of counting the number of primary electrons that are released toward the target cell. Such a method has not been conventionally employed at all.

In addition, by placing the PIPS detector at the cell position above the pinhole, the beam variation before and after cell irradiation can be measured repeatedly to confirm the average characteristic of the beam incident on the cell in this experiment. In the case of using a PIPS detector, there is an advantage in that information on energy as well as the number of radiation particles constituting the beam can be obtained. Based on the response function of the PIPS detector, the number of radiation particles entering the target cell can be calculated and the energy spectrum of the incident beam can be obtained.

8 does not limit the generated radiation to electrons in a preferred embodiment of the individual cell irradiation microbeam device according to the present invention, in particular energy measured using a PIPS detector when using a 70 keV electron beam. Spectra are shown in comparison with simulation results using EGS codes.

Referring to FIG. 8, it can be seen that the continuous tail formed wide in the region at the full energy absorption peak and the low energy of the peak. The low-energy continuous tail is formed by electrons entering the detector after being scattered in the backscattering or pinhole structure material at the PIPS detector surface, and when the secondary radiation or braking radiation formed inside the PIPS detector is partially absorbed by the detector as it leaves the detector. do. The total energy absorption peak accounts for approximately 76% of the total area, the contribution from backscattering or braking radiation by the detector to approximately 16%, and the rest is attributed to scattering in the pinhole structure material. Statistical characteristics of the beam incident on the cell with the PIPS detector removed from the beam spectrum measured by the PIPS detector can be assumed.

Figure 9 shows the fluctuation characteristics of the number of electrons incident on a cell in a state in which a preferred embodiment of the individual cell irradiation microbeam device according to the present invention is operated in the pulse mode. When generating an electron beam with energy of 70 keV in pulse mode with a beam width of 2 msec at a repetition rate of 200 Hz, the total energy absorption peak area measured using a multichannel counter is shown in FIG. A stable occurrence of could be assumed.

The intensity and energy distribution of the beam in a two-dimensional direction parallel to the surface of the vacuum window at the cell vessel position above the vacuum window was determined by measuring using a beam scanning method.

FIG. 10 shows the spatial distribution of the electron beam intensity passing through the microbeam generating unit, which constitutes a preferred embodiment of the microbeam device for individual cell irradiation according to the present invention.

10, the full width at half maximum is about 5 mu m.

10, it can be seen that the microbeam device for irradiating individual cells according to the present invention can selectively irradiate the microbeams to the cells.

FIG. 11 is a simulation of the total cell dose distribution around the cells evaluated using EGS codes for electron beams of 30, 50 and 70 keV in order from the top, respectively, in a preferred embodiment of the individual cell irradiation microbeam device according to the present invention. The results are shown.

Simulation calculations were performed taking into account beam transport boxes, pinhole structures and frame materials and structures, mylar vacuum windows and mylar cell container bottoms and cell cultures, assuming beams traveling parallel to the target cell direction in the vacuum compartment. Total absorbed dose distribution data simulated using EGS are converted into matrices in ORIGIN software and presented in the form of contours.

11, it can be seen that the microbeam device for irradiating individual cells according to the present invention can selectively irradiate the microbeams to the cells.

So far I looked at the center of the preferred embodiment for the present invention. Those skilled in the art will appreciate that the present invention can be implemented in a modified form without departing from the essential features of the present invention. Therefore, the disclosed embodiments should be considered in descriptive sense only and not for purposes of limitation. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the scope will be construed as being included in the present invention.

According to the micro-beam apparatus and method for irradiating individual cells according to the present invention, not only the radiation of low radiation energy such as electron beam can be selectively exposed in units of cells, but also whether the cells are exposed or not. You can monitor in real time. Accordingly, the microbeam apparatus and method for individual cell irradiation according to the present invention can be a physical support base for radiobiological experiments to produce information in a form more effective for risk analysis of radiation, in particular low dose radiation.

Claims (11)

A beam generator for generating a beam, One side is connected to the beam generator and passes through a beam generated and propagated from the beam generator, and the other side transports a beam including a vacuum window, A micro beam generator disposed on the other side of the beam transport box to generate a micro beam from the beam, and A cell irradiation table including a cell container and a cell container mount disposed on a micro beam generating unit opposite the beam transport box, The inner wall of the beam transport box is lined with mumetal, individual micro-irradiation device for irradiation. The method of claim 1, And said beam is selected from the group consisting of electron beam, proton beam, α-ray beam and heavy charged particle beam. The method of claim 1, And said beam is an electron beam. The method of claim 1, A cell position control unit for adjusting a relative position of the cell container with respect to the micro beam, a cell image acquisition unit including a fluorescence microscope, a processing unit of the obtained image, a control unit of the beam generation unit, and the beam generation unit and a beam transport box The micro-beam device for individual cell irradiation further comprises at least one of the connected vacuum pump. The method of claim 1, The mumetal is lined to a thickness of 0.01 ~ 0.5 inch microbeam device for individual cell irradiation. The method of claim 1, The upper end of the vacuum window is flattened, the mylar thin film device for individual cell irradiation, characterized in that the Mylar thin film is fixed to the upper end of the vacuum window. The method of claim 6, The fixing of the mylar thin film micro beam device for individual cell irradiation, characterized in that using the O-ring and the fixing ring. The method of claim 1, The bottom of the cell container is a micro-beam device for individual cell irradiation, characterized in that consisting of a Mylar thin film. The method of claim 1, And a microchannel plate disposed below the bottom of the vacuum window. Generating a beam by the beam generator of the micro-beam device for irradiating individual cells according to any one of claims 1 to 9; Passing the generated beam through a beam transport box and generating a micro beam by a micro beam generator; And Irradiating the generated microbeams with cells in a cell container; Method of irradiating a micro beam to individual cells comprising a. The method of claim 10, Monitoring average microbeam characteristics before and after irradiation using a passivated implanted planar silicon (PIPS) detector, or monitoring the microbeam irradiated to the cells in real time using a microchannel plate. How to feature.

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