KR20070018125A - Method and Apparatus of magnetic force-driven rotational Couette flow system - Google Patents

Abstract

The present invention relates to an apparatus and a method for producing a rotatable Couette flow by magnetic force, and the constituent apparatus includes a sample storage chamber, a Couette fluidizer, a magnetic rotation induction device, and the like. The present invention relates to a device for generating a rotating Couette flow inside the sample chamber through a rotation induction device using a magnetic force to a micro device containing a very small amount of sample liquid, without a mechanical power transmission method, the control and measurement of the rotation speed Through this, the shear force, shear rate, viscosity, degree of aggregation, and the like acting on the sample liquid can be measured. A feature of the present invention is that it can handle a very small amount of sample liquid, all the components of the sample liquid contact portion can be disposed of after use can be discarded after use, it is characterized in that the operation is very simple.

Microdevice, magnetic force, Couette flow, disposable

Description

Magnetically driven rotary Couette flow system and method {Method and Apparatus of magnetic force-driven rotational Couette flow system}

1 is a schematic diagram showing the configuration of a magnetic drive rotation type Kuet flow apparatus according to the present invention

2 is a top view of a disposable kit according to the embodiment of the device shown in FIG.

3 is a schematic diagram of a microchannel comprising a circular flat plate Couette fluidizer rotating by a magnetic rotating guidance device in accordance with an embodiment of the device shown in FIG.

4 is a schematic diagram according to various embodiments of a rotating flatbed Cuet fluidizer according to the embodiment of the apparatus shown in FIG.

5 is a schematic diagram of a microchannel including a cylindrical Kuet flowr rotating by a rotary induction device in a double concentric tube structure according to the embodiment of the device shown in FIG.

6 is a schematic diagram according to various embodiments of a cylindrical Couette fluidizer according to the embodiment of the apparatus shown in FIG.

7 is a schematic diagram of a device for measuring blood viscosity and blood cell aggregation rate according to an embodiment of the device shown in FIG.

8 is a schematic diagram of a device for measuring blood viscosity and blood cell aggregation rate according to an embodiment of the apparatus shown in FIG.

9 is a graph showing blood cell aggregation rate measurement results over time according to an embodiment of the apparatus shown in FIG. 7 or 8.

10 is a graph showing the results of measuring the viscosity of a liquid according to the rotation speed difference according to the embodiment of the apparatus shown in FIG. 7 or FIG.

The present invention relates to a magnetically driven rotary Couette flow device, and to a flow device capable of applying a constant or variable shear flow to a sample liquid in a microchannel using a rotation induction device.

The Kuet flow device is a representative flow control device and has been used for various purposes. In particular, rotational Couette flow is widely used to measure the viscosity of fluids, and is commonly found in expensive commercial viscometers. In recent years, the development of a measuring device for the aggregation rate of blood cells has been attempted, as the aggregation rate of blood cells is known as a direct influence factor on the viscosity and rheological properties of blood. 307-314, 1998) reported that the platelet plate shearing system can be used to generate rotary cue shear flow and to measure the hemagglutination rate. In addition, in the study of erythrocyte agglutination system published in the Journal of Clinical Hemorheology and Microcirculation (Vol. 25, pp. 1-11, 2001), the rotating Kuet shear using a double concentric tubular system (Cup-and-bob system) It is reported that the flow can be generated and the hemagglutination rate can be measured.

As such, the rotating Kuet flow is a representative flow control mechanism used in blood viscometers and hemagglutination systems, and the hemoglobin aggregation system will be briefly described as follows. That is, when a blood sample is filled between the double circular plates, light such as a laser or an LED is irradiated onto the blood sample, and light obtained through transmission or reverse scattering of the irradiated light is acquired by using a photodiode sensor. A technique for measuring the aggregation rate of blood cells by processing and analyzing light intensity with a computer has been published. At this time, prior to the measurement, at least one of the two plates is rotated at high speed to disperse the aggregated blood cells, thereby creating a rotating Couette flow condition and generating a shear flow so that the shear rate is at least 500 (1 / s). It is maintained for more than 5 seconds. When the high-speed rotational motion is suddenly stopped, the shear flow is also rapidly stopped within 0.1 seconds. From this time, hemagglutination progresses, and the change in time is measured by the intensity of light. This measurement is a sig- lectogram that shows the change of aggregation rate over time and is known as the most scientific method of blood cell aggregation and has been adopted by many diagnostic blood test laboratories.

However, such a hemagglutination measurement system requires a complex rotary Couette flow system, which increases the price or system size. Because of this, a sample amount for one measurement takes a large amount of 2ml, and also requires a separate vacuum pump and washing liquid because it requires a separate wash after use. For this reason, the measurement of hemagglutination rate has not been practically used because it is very inconvenient to use in the clinical field despite the importance of measurement.

Agglomeration rate measurement using the photometric method is already known. However, these disclosed techniques are difficult to overcome in the clinical field due to the complex rotational shear flow or noise-induced vibration in the technique of eliminating initial hemagglutination of blood samples and fully dispersing blood cells. This exists. In addition, the existing device is a disposable kit is not possible to handle the blood sample is very cumbersome post-test cleaning process has a disadvantage that takes a certain amount of blood samples.

SUMMARY OF THE INVENTION The present invention has been made with the above circumstances in mind, and is directed to the technical problem of inventing an apparatus and method for generating a magnetically driven rotary Couette flow implemented in a disposable microchip.

Another technical problem of the present invention is to replace the existing technology by applying such a magnetic drive rotary Kuet flow apparatus to a variety of existing applications. For example, the present invention is to invent a new method and apparatus for dispersing aggregated blood cells prior to measurement by applying to the measurement of hemagglutination, and another method is to invent a method and apparatus for measuring the viscosity of a sample liquid. In addition, such a device has a technical problem of inventing a device and a related measuring method including a blood cell aggregation rate measuring kit that can be used for single use because of its simple structure, very easy operation and low production cost.

This technical problem of the present invention can be solved by providing a method for generating a magnetically driven rotary Couette flow by the following technical configuration.

The present invention relates to a method and apparatus for generating Couette flow in a sample liquid in a micro device using a magnetically driven rotational induction mechanism. In order to achieve this purpose, the sample inlet 11 into which the blood sample 14 is injected, the sample storage chamber 13 storing the sample liquid and generating the cuet flow therethrough, and the cousin existing in the sample storage chamber and inducing rotational movement. Although it is spatially separated from the edge fluid 60 and the disposable part 10 including all the parts contacting with the sample liquid in this way, it is attached to one side of the sample induction chamber and the rotation induction device 20 for applying rotation to generate a light source. Device 31 for detecting light, device 32 for detecting light, processor 51 for storing, calculating, and processing data measured by the light sensing device, device 52 for displaying the result of the calculation on the screen, and storing data. And the output device 54 for outputting data and the like.

A schematic configuration diagram of the magnetically driven rotary Couette flow apparatus of the present invention is shown in FIG. 1. 2 and 3 are a plan view and a side view of the disposable kit of the magnetically driven rotary Couette flow apparatus according to the embodiment of the apparatus shown in FIG. FIG. 4 is a schematic view of various embodiments of a circular flat Couette fluidizer in the disposable Couette flow kit shown in FIG. In addition, Figure 5 is a schematic diagram of a double concentric tubular flow apparatus and Figure 6 is a schematic diagram according to various embodiments of the cylindrical cue flowr in the disposable Couette flow kit shown in FIG. Referring to Figure 3 specifically described the operation principle and measuring method of the magnetic drive rotation type Kuet flow generating apparatus as follows.

First, a sample liquid 14 such as blood is injected into the sample inlet 11. At this time, the sample liquid injected into the sample inlet is introduced into the sample storage chamber 13 through the microfluidic tube 12 due to the capillary effect. In this case, when the sample solution is blood, an appropriate amount of anticoagulant is coated in the sample storage chamber to be used as a sample by directly collecting blood from the fingertip. Next, when the magnetically driven rotary induction device 20 using an electromagnet or a rotating permanent magnet is operated, the Kuet flowr 60 made of a material affected by the magnetic field inside the sample storage chamber is induced to rotate. During the rotational movement of the Kuet fluid 60, the sample liquid in the sample storage chamber is subjected to shear flow by the rotating plate and the fixed wall thereon. This flow is called parallel-plate rotational Couette flow, and the shear rate inside the flow field can be precisely controlled by the rotational speed, the radius of rotation, and the gap between the two plates. That is, the shear rate is proportional to the rotational speed and the rotational radius and inversely proportional to the gap between the rotating plate 61 and the fixed top wall. The rotational speed of the optical sensor 32, such as a photodiode or the like, is reflected by a light source 31 such as a laser diode or a light emitting diode (LED) attached to one side of the sample storage chamber shown in FIG. By sensing, the rotation speed per unit time can be measured.

4 shows various examples of the Kuet flowr 60 according to the embodiment of FIG. 3, which is composed of a metal material affected by a magnetic field and has an example of a tack-like shape on the left side. In addition, in the center of FIG. 4, a tack shape is formed of a non-ferrous metal material or plastic, which is not influenced by a magnetic field, and a assembler is assembled by attaching a strip of metal material affected by the magnetic field to a lower portion of the disc. It was. In this case, the induction rotational motion is more stable and lighter than the former, so that a slip phenomenon due to the high speed rotation of the rotation induction device is reduced. On the other hand, in order to minimize the vibration of the rotational flow caused by the magnetic field an example of the Kuet flowr 60 attached to the central axis on the upper plate is shown in the right figure. In this case, the material may be composed of a material affected by the magnetic field or a non-metallic material, and then the strip affected by the magnetic field may be attached to the lower part of the disc. In the case of the right embodiment, it is not strictly a parallel flat rotary flow, but if the central axis is sufficiently thin, it can be used accordingly.

Similarly, referring to FIG. 5, the operation principle and method of the concentric rotational Couette flow generating device, which is another embodiment of the self-driven Couette flow generating device of the present invention, are described as follows. First, a sample liquid 14 such as blood is injected into the sample inlet 11. At this time, the sample liquid injected into the sample inlet is introduced into the sample storage chamber 13 by gravity. In this case, when the sample solution is blood, an appropriate amount of anticoagulant is coated on the inner wall of the sample storage chamber in order to use the sample directly at the fingertips. Rotating movement is induced by the rotation induction device 20 is a Kuet flowr 60 in the sample storage chamber and has a circular rod shape. During the rotational movement of the Kuet fluid 60, the sample liquid in the sample storage chamber is subjected to shear flow between the rotating inner cylinder and the fixed outer cylinder. This flow is called double concentric tube flow, and the shear rate inside the flow field can be precisely controlled by the rotational speed, the radius of rotation, and the gap between the two plates. In particular, when the radius ratio of the inner tube to the outer tube is 0.99 or more, the shear rate is directly proportional to the rotational speed. The rotational speed is that the light sensor 32 such as a photodiode or the like is irradiated by the light source 31 such as a laser diode or a light emitting diode (LED) attached to one side of the sample storage chamber shown in FIG. 8. By sensing, the rotation speed per unit time can be measured.

FIG. 6 shows various examples of the guet flowr 60 according to the embodiment of FIG. 5. An example having a shape consisting of a circular center axis and a circular plate is shown on the left side. have. In the center of FIG. 6, a circular center axis and a circular plate shape are formed of a non-ferrous metal material or plastic, which is not affected by the magnetic field, and a strip of metal material affected by the magnetic field is attached and assembled to the upper part of the disc. The Kuet flowr is shown. In this case, the induction rotational motion is more stable and lighter than the former, and the slip phenomenon due to the high speed rotation of the rotation induction device is eliminated. On the other hand, in the figure on the right, a non-ferrous metal material or plastic, which is not affected by the magnetic field, shows a Kuet flowr in which a circular center axis and a strip of metal material affected by the magnetic field are attached to the central axis.

The magnetically driven Couette flow generating device as described above can be applied to the blood cell aggregation system, specifically, with reference to FIGS. 7 and 8 Blood cells using a rotating cue flow generating device which is one embodiment of the disposable Couette flow generating device of the present invention The principle and method of operation of the coagulation system are as follows. 7 and 8 are schematic diagrams of an apparatus for measuring blood cell aggregation rate when the sample solution according to the example of FIG. 3 is blood. Disposable kit 10 is formed by the kit holder 40 to maintain a constant gap between the kit unit, the sensor unit and the rotation induction device 20. As the sample solution is injected, the Kuet's fluid is slowly rotated for a period of time to mix blood and anticoagulant coated on the sample chamber surface. Thereafter, the Kuet flowr is rotated at a very high speed to maintain a shear rate of 500 (1 / s) or more for 5 seconds, and then suddenly stop. At this time, the sample liquid in the sample chamber is known to stop within 0.1 second. At this time, it is a device for measuring the backscattering of the light emitted from the light source or the intensity of the transmitted light with time.

8 is a graph showing the results according to the embodiment of FIG. 7, that is, the intensity of backscattered light over time. This is called a syllectogram. Prior to applying a strong rotating cue flow, the blood cells are agglomerated, so the surface area ratio of the blood cells is relatively low, so that most of the light emitted from the light source is transmitted and the amount of backscattering is low. By rotating the Kuet fluid, the aggregates of blood cells are dispersed within 2 to 3 seconds, increasing the surface area ratio of blood cells. Therefore, the amount of backscattered light gradually increases exponentially and reaches its maximum value. At this time, when the rotating cue flow is stopped, the aggregation of blood cells increases exponentially, and thus the intensity of backscattered light decreases exponentially. This is measured by the optical sensor 32 and the measured signal value is stored in the processor 51 at each time and analyzed and converted to the cohesion rate characteristic of the blood sample, and the value is displayed on the output device 52 such as a screen. Done. Methods for analyzing these selectorgrams have already been published in the Journal of Clinical Hemorheology and Microcirculation (Vol. 25, pp. 1-11, 2001), which can be used to determine various hemagglutination indexes.

In FIG. 7, the measurement was performed using backscattered light, but a method of measuring blood cell aggregation rate using a light transmission method is already known. That is, the only difference is that the light emitted from the light source is scattered in the blood cells and collected by the optical sensor.

The magnetically driven Couette flow generating device as described above can be applied to the purpose of measuring the liquid viscosity. First, referring to FIG. 8, the operation principle and method of the viscometer using the disposable Couette flow generating device of the present invention will be described below. When the disposable kit 10 is inserted into the kit holder 40, the kit part firmly forms a support part, and also maintains a constant distance from the sensor part 32 and the rotation induction device 20. After injecting the sample liquid, the quet fluidizer 60 is rotated at various rotational speeds using the magnetic rotary induction apparatus 20. At this time, the frictional shear force is applied to the Couette fluid due to the viscosity of the sample liquid, and thus a slip phenomenon occurs in the rotation of the Couette fluidizer and the rotating induction device. It shows the difference with the rotation speed of. This difference in rotational speed can be explained in relation to the viscosity of the liquid and can be used to measure the viscosity of the sample liquid as shown in FIG. 10. In particular, the advantage of the present invention that the shear rate is changed according to the rotational speed can be used to measure the viscosity of the sample liquid at various shear rates, it is suitable for measuring the viscosity of a liquid having a non-Newtonian viscosity, such as blood. In addition, the volume of the sample chamber can be minimized, so that the requirement of the sample liquid can be limited to several microliters, which can be usefully used for measuring the blood viscosity.

As shown in FIG. 2 and FIG. 3, the disposable kit for directly contacting the sample liquid in the device configuration of the present invention is the sample inlet 11, microfluidic tube 12, the sample reservoir 13 is made of silicon, At least one selected from candidate groups such as quartz, silica, glass, laser processable polymers, injection molded polymers, and ceramics may be produced. Such a disposable kit can be manufactured as a disposable product that can be disposed of after use. That is, the structure as shown in FIG. 3 can be easily processed using a micro-injection method using a plastic material as a substrate. Such plastic base material is very economically suitable for single use, so it is very convenient to dispose after measurement if there is concern about contamination of pathogens such as blood. At this time, the disposable kit may be manufactured in one piece or may be assembled in a form in which the upper plate 15 and the lower plate 16 are separated and then bonded. Similarly, microchannel processing is possible for materials such as silicon, quartz, silica, and glass using MASK made through the LIGA process. Therefore, the material of the disposable kit is selected from a candidate group of silicon, quartz, silica, glass, laser processable polymer, injection molding polymer and ceramic.

Sample storage chamber 13 shown in Figure 3 is a sample chamber is a circular chamber shape, the size of the storage chamber can be used in various sizes depending on the purpose of use, but the diameter is generally about 1 ~ 10 millimeters, depth is 0.1 ~ 5 It is about millimeters. Therefore, the sample storage room can be designed so that the blood sample amount can be measured with only a few microliters. In this case, in order to allow the blood to flow into the sample storage chamber well, an exhaust hole 17 may be secured in the sample storage chamber to prevent the gas present therein from being compressed. Therefore, the blood sample can be filled in the sample reservoir. The top or bottom plate of the sample reservoir should be optically transparent, and the degree of aggregation of blood cells in the blood placed in the sample reservoir can be measured by the amount of scattered light.

Disposable disc-shaped Kuet flowr 60 shown in FIG. 4 is a rotary motion by the rotation induction device 20, when the rotation induction device uses the effect of the magnetic field that changes with time, it affects the magnetic field Any object of appropriate size and shape may be used. The size of the disposable Couette fluidizer should be designed in proportion to the size of the sample reservoir. The radius of the rotating plate 61 is about 1 to 9 millimeters and the length of the central axis 62 is about 0.5 to 5 millimeters. The Kuet fluid may be shaped like a tack using ferrous metals that are affected by magnetic fields. In addition, it is possible to form a tack using a plastic or non-ferrous metal, it can be used by symmetrically attaching a long strip 63 of a thin, ferrous metal to the lower center of the circular plate (61). In the latter case, the rotational motion can be stably induced in the rotating induction magnetic field, and the weight of the Kuet's fluid is lightened, thereby reducing the rotational slip with the rotational induction device.

The disposable cylindrical cue floater 60 shown in FIG. 6 also rotates by the rotation induction device 20, and when the rotation induction device uses the effect of the magnetic field that changes with time, Any object of appropriate size and shape that is affected may be used. The size of the disposable Couette fluidizer should be designed in proportion to the size of the sample reservoir. The radius of the rotating tube 65 is about 0.5 to 10 millimeters and the length is about 5 to 20 millimeters. Such Kuet fluid may use all or part of magnetization materials such as ferrous metals affected by magnetic fields. In addition, to form a cylindrical shape using a plastic or non-ferrous metal, it is possible to use a thin strip of ferrous metal 66 is inserted into the circular tube or attached to the lower center of the circular plate 68. In order to minimize friction of the central axis during the rotational movement, the ends of the upper 67 and lower 65 of the circular central axis have a pointed shape.

The central axis 62 of the circular plate Kuet flowr of FIG. 4 should be located at the position of the center of gravity as well as at the geometric center of the circular plate 61, since the vibration can be minimized during the rotational movement. In addition, in order to reduce the friction between the central axis 62 of the Kuet flowr and the groove 14 of the disposable kit, it is possible to minimize the rotating contact portion by sharpening the end of the central axis 62 of the Kuet flowr.

The Kuet fluid is preferably coated with a blood compatible substance such as Teflon or a stable polymer in the blood so as not to affect the blood. In addition, materials and processing should be inexpensive to allow for the disposal of such Couette flowers as well as disposables after use.

Through the rotating cue flow using the rotating induction apparatus 20 and the cue flowr 60, the blood in the sample storage chamber is subjected to agitation or shear flow. In this case, in order to remove hemagglutination and uniformly disperse blood cells within a short time, the strength of shear flow should be properly designed. At this time, excessive shear flow may cause damage to blood cells, which may affect the aggregation of blood cells. In addition, since the magnetic field of the rotation induction apparatus may affect blood cell aggregation, a magnetic shield plate may be inserted into the sample storage chamber and the rotation induction apparatus to prevent such interference.

The magnetically driven rotation induction device 20 shown in FIG. 3 induces an appropriate rotational motion to the cue floater 60 to generate a rotatable cue flow. One example of the configuration of the rotation induction device 20 may be a small motor, a disc, a permanent magnet, or the like. At this time, the permanent magnet is symmetrically attached to the disc, and the permanent magnet is rotated to the rotational speed of the motor, thereby affecting the Kuet flowr in the sample chamber. As another example of such a configuration of the rotation induction device, it can be configured even without a driving device that rotates using an electromagnet. That is, by repeatedly changing the direction of the magnetic field using the electromagnet can achieve the same effect as the principle of rotating the permanent magnet.

The method and apparatus for generating a cue flow using the rotation induction device and the cue flowr are characterized in that the cue flow can be implemented simply and very effectively. This feature is more versatile in that Couette flow is achieved by transmitting power by magnetic force without mechanical power transmission. That is, the part which contacts a sample liquid can be made disposable. In addition, the device can be micronized to apply Couette flow to small amounts of sample and provide the core technology to perform related experiments and measurements. This technique can be very useful especially when collecting a small amount at the fingertips, such as blood.

Using a magnetically driven Kuet flowr as an example of the present invention, a blood cell aggregation rate measuring instrument is presented above. This solution solves the disadvantages of the existing technology by using the CUET flow which can remove the hemagglutination in a short time as it is and the miniaturization of such a CUET flow device. In other words, in the mechanism of dispersing aggregated blood cells, which was the biggest problem of the existing technology, the rotating shear flow method using a double concentric tube or a double plate is complicated, requires expensive equipment, and can not be easily cleaned. .

The light sources shown in FIGS. 1 and 5 may optionally be used in any of the groups of light emitting diodes (LEDs), laser diodes, lasers, and the like. The optical sensors may include photodiodes and CCD sensors, and At least one of the candidate groups such as Array may be selectively used.

On the other hand, since the hemagglutination characteristic of blood has a characteristic that varies with temperature, it is necessary to control the measurement temperature. Therefore, even in the configuration of the present invention, it is possible to preheat the temperature of the sample and the disposable kit to a predetermined temperature by mounting a thermo-electric component for maintaining a proper temperature by heating the disposable kit or by an optical method such as a halogen lamp. Can be configured to

According to the above configuration and operation, the present invention has the advantage that it is possible to generate a rotary cue flow by using a magnetic driving rotary induction device and a cue flowr in the sample liquid, and such a cue flow device has the advantage of being disposable and miniaturized. In addition, the sample volume can be minimized to a few microliters. Particularly, the device of the present invention does not come into contact with the sample liquid other than the disposable kit, and all the portions in contact with the sample liquid can be manufactured as a disposable kit. It has advantages

It is apparent to those skilled in the art that the present invention is not limited to the described embodiments, and that various modifications and variations can be made without departing from the spirit and scope of the present invention. In particular, it is apparent to those skilled in the art that the present invention can also be applied to any technique that uses a rotating flat Kuet flow. Therefore, such modifications or variations will have to belong to the claims of the present invention.

Claims (12)

An apparatus for generating a magnetically driven rotary cue flow, the configuration of which is as follows. Sample inlet; A sample storage chamber connected with the injection hole and receiving the sample liquid injected; A rotation induction device for inducing rotation in the sample storage chamber; A kuet flowr existing in the sample storage chamber and configured to rotate by the rotation induction device; A light source positioned on one side of the sample storage chamber; An optical sensor for sensing light emitted from the light source to the sample; A processor connected to the rotating device and an optical sensor, inducing rotation according to time, and calculating a shear rate by analyzing the detected light according to time; Rotary Couette flow generating device characterized in that it comprises an output device for indicating the shear rate calculated from the processor The method of claim 1, Rotational Couette flow generating device, characterized in that the power transmission between the rotation induction device and the Kuet flowr is transmitted without direct mechanical fastening The method of claim 2, As a method of power transmission, the rotation induction device 20 changes the magnetic field over time to induce rotational motion, and the Kuet fluid 60 is characterized in that the rotational motion is induced by the influence of this magnetic force Magnetically Driven Rotary Couette Flow Generator The method of claim 3, wherein The rotation induction device 20 is a magnetic drive rotation type Kuet flow generating device, characterized in that composed of at least one of a mechanical rotational motion method of the permanent magnet or an electromagnetic magnet method for electrically changing the direction of the magnetic field. The method of claim 3, wherein The kuet flowr 21, Magnetically driven rotary quet flow generating device, characterized in that composed of at least one of a disk-shaped quet flowr, a cylindrical tube flow. The method of claim 5, Disc type Kuet flowr is a rotating Kuet flow generating device characterized by a tack-shaped shape consisting of a disc and the center of rotation. The method of claim 5, The cylindrical cue flowr is a rotary cue flow generating device, characterized in that composed of a central circular rod, a rotation induction reaction plate and the upper central axis. The method according to claim 6 and 7, The Kuet flowr is a magnetically driven rotational Kuet flow generating device, characterized in that at least a portion of the Kuet flowr is made of a material that is magnetized under the influence of the magnetic field, so that the rotational motion is induced by the rotation induction device. The method according to claim 6 and 7, The rotational contact of the Kuet fluid is Magnetically driven rotary cue flow generating device, characterized in that it is configured in the shape of a sharp awl in order to minimize the contact friction resistance with the support during the rotational movement. The method of claim 1, The optical sensor 71, A rotating Kuet flow generating device, characterized in that composed of at least one of a photodiode or a CCD sensor array. The method of claim 1, The kuet flow generating device, Rotary Couette flow generating device, characterized in that the viscosity of the sample liquid can be determined by comparing the rotational speed of the rotary flow apparatus 20 and the rotation speed difference of the Couette fluidizer (60). The method of claim 1, The kuet flow generating device, Rotary Couette flow generating device, characterized in that the degree of aggregation of the sample liquid can be measured by recording the time of light irradiated on the sample liquid.

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