KR20070010776A - Peroxy radical calibration system and method - Google Patents

Abstract

An apparatus and a method for measuring a Peroxy radical are provided to reduce the measurement cost by measuring the Peroxy radical with reference to the initial density. An apparatus for measuring a Peroxy radical includes a solution conveying section(10), a radical generating section(20), a knot type reactor(30), a gas/liquid separating section(40), a snail type reactor(50), a light emission detecting section(60), a signal analyzing section(70), and a plurality of valves(V1,V2,V3,V4). The signal analyzing section detects the density of the initial Peroxy radical by analyzing an electrical signal output through the light emission section. The signal analyzing section detects the density of the Peroxy radical in a measured object by comparing the strength of the fluorescence of the measured object with a set strength of fluorescence.

Description

Peroxy radical calibration system and method {peroxy radical calibration system and method}

1 is a block diagram showing a peroxy radical measuring apparatus according to an embodiment of the present invention.

2 is a perspective view showing a peroxy radical generating device according to an embodiment of the present invention.

3 is a perspective view illustrating a knotted reactor according to one embodiment of the present invention.

4 is a perspective view illustrating a snail reactor according to an embodiment of the present invention.

5 is a linear equation graph showing the relationship between the signal ratio and the knotted reactor length according to pH conditions according to an embodiment of the present invention.

6 is a flowchart illustrating a peroxy radical measuring method according to an embodiment of the present invention.

* Explanation of symbols for main parts of drawings *

10: solution carrying part 20: peroxy radical generating part

30: knot reactor 40: gas phase / liquid phase separation

50: snail reactor 60: emission amount detection unit

70: signal analysis section V1, V2, V3, V4: first to fourth valve

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a peroxy radical measuring device and a method thereof, and more particularly to a peroxy radical for measuring peroxy radicals present in a very small amount of time (a few seconds to a few minutes) in the atmosphere or water. Roxy radical measuring device and method thereof.

In general, peroxy radicals are one of the free radicals in the atmosphere and have been known to have significant effects on the formation of smog in metropolitan areas and ozone production in the troposphere through the formation of nitrogen oxides. In addition, peroxy radicals are reactive intermediate species that occur in advanced oxidative processes (AOP), which are wastewater and water treatment processes, and are produced simultaneously with the consumption of oxygen in water or during decomposition of organic matter. Have. In particular, the peroxy radical is one of the active oxygen species (ROS) in the field of biotechnology research has been studied for more than 50 years.

A study on the measurement of peroxy radicals was recently reported in 2003 by An et al. (Anal. Chem, 2003, 75, pp, 4969-4700). Conventional peroxy radical measurements reported to date include Laser Induced Fluorescence (LIF), Chemical Amplification Technique, Electron Spin Resonance (ESR), and Chemical Ionization Mass Spectrometry of Peroxy Radicals Peroxy radical chemical ionization mass spectroscopy (PerCIMS), followed by MCLA (2-methyl-6- (p-methoxyphenyl) -3, 7-dihydroimidazo- [1,2-alpyrazin-3-one) Flow injection analysis with chemiluminescence detection has been developed.

However, despite the excellent sensitivity, conventional peroxy radical measurement is still under development due to the disturbing effects caused by ozone, nitrogen dioxide, etc., serious errors, complicated operation, and the inconvenience of maintaining sub-zero temperature during analysis. . In particular, the most serious problems of peroxy radical measurement techniques are that it is not easy to prepare absolute calibration curves for quantification, the measurement equipment is complicated, transport / installation for mobile measurement is difficult, and measurement equipment is caused by particulate matter and trace gases in the atmosphere. There is uncertainty in measurement because it causes serious interference effect, and the measuring equipment used is expensive.

In addition, the method of measuring peroxy radicals applied in water was reported in Bielsky et al. (J. Phys. Chem. Ref. Data., 1985, 14) in 1985, and summarized the research progress reported in this journal. According to the results, using a specific material such as tetranitromethane, Cytochrome C, and Nitro Blue Tetrazolium, absorbance measurement technology of microsecond time resolution, electron spin Electrospinning resonance (ESR) and chemical luminescence techniques (Fluorescence) using fluorescence of specific materials have been used. However, the measurement techniques such as absorbance and ESR are not suitable for use in trace analysis because they are less sensitive than fluorescence detection, and there is no specific and general methodology related to quantification.

Accordingly, the present invention has been made to solve the above problems, an object of the present invention is to decompose the pH-controlled aqueous solution to produce peroxy radicals, and to measure the concentration change of the peroxy radicals to calculate the half-life This half-life is then used to detect and calibrate the initial concentration of peroxy radicals, and to measure the concentration of peroxy radicals contained in atmospheric or liquid samples based on the calibrated initial concentration. .

Peroxy radical measuring apparatus according to the present invention for achieving the above object is a solution carrier for separating and transporting each of the various transport solutions required for the chemical reaction and the luminescent solution to form a light emitting body by reacting with the peroxy radical; A peroxy radical generating unit for generating peroxy radicals by photolysing an aqueous pH-adjusted aqueous solution to calibrate peroxy radicals; A knotted reactor for varying the concentration of the peroxy radicals by passing the peroxy radicals through knotted tubes of different lengths to produce a half-life for detecting the concentration of the initial peroxy radicals; A gas phase / liquid phase separation unit that separates the air sample or liquid sample mixed with the transport solution carried in the solution transport unit into the gas phase and the liquid phase and discharges the gas phase to the outside; A snail reactor for forming a fluorescent emitter by reacting a solution passing through a knotted reactor or gas phase / liquid phase separation unit with a luminescent solution carried through a solution carrier; A light emission amount detector for measuring a fluorescence intensity of the fluorescent light emitting body formed in the snail reactor and outputting an electric signal; Analyze the electrical signal output through the light emission detection unit to detect the concentration of the initial peroxy radicals, set the fluorescence intensity of the initially detected fluorescent emitters to the initial peroxy radical concentration and calibrate, and then the fluorescence intensity and calibration of the measurement target A signal analysis unit for detecting a concentration of peroxy radicals included in the measurement target compared to the fluorescence intensity which has been converted; It is characterized by consisting of a plurality of valves that are opened and closed to calibrate by measuring the concentration of the peroxy radicals generated by photolysis of the aqueous solution or to measure the concentration of the peroxy radicals contained in the air sample or the liquid sample.

In addition, the measuring method using a peroxy radical generating device of the present invention comprises the steps of photolysis of the pH-controlled aqueous solution introduced from the outside to calibrate the peroxy radicals to generate peroxy radicals; Adjusting the length of the knotted reactor to vary the concentration of the peroxy radicals produced above; Reacting the peroxy radicals with the luminescent solution to form a fluorescent emitter, and detecting a change in concentration of the peroxy radical by detecting the fluorescence intensity of the fluorescent emitter; Calculating a linear equation by comparing the detected fluorescence intensity with a signal ratio according to pH conditions and a length of the knotted reactor as X and Y axes, respectively; Calculating a half-life using the slopes and intercepts calculated in the linear equations; Detecting the initial peroxy radical concentration using the calculated half-life; Setting the fluorescence intensity of the initially detected fluorescent emitter to an initial peroxy radical concentration to complete calibration; Reacting the measurement object with the MCLA solution to form a fluorescent emitter, and detecting the fluorescence intensity of the fluorescent emitter; And detecting the peroxy radical concentration by comparing the fluorescence intensity of the set initial peroxy radical concentration with the fluorescence intensity of the measurement target.

Hereinafter, a preferred embodiment of the peroxy radical measuring device according to the present invention will be described in detail with reference to the accompanying drawings.

1 is a block diagram illustrating a peroxy radical measuring device, FIG. 2 is a perspective view showing a peroxy radical generating device, FIG. 3 is a perspective view showing a knotted reactor, and FIG. 4 shows a cochlear reactor. 5 is a perspective diagram illustrating a relationship between a signal ratio and a knotted reactor length according to pH conditions according to an embodiment of the present invention.

As shown in FIG. 1, the peroxy radical measuring apparatus of the present invention includes a solution carrying unit 10, a peroxy radical generating unit 20, a knotted reactor 30, and a gas phase / liquid phase separating unit 40. ), A cochlear reactor 50, a light emission amount detecting unit 60, a signal analyzing unit 70, and a plurality of valves V1 to V4.

The solution transport unit 10 separately transports various transport solutions required for chemical reactions and luminescent solutions that react with peroxy radicals to form light emitters.

For example, the solution transport unit 10 carries a transport solution to one side of the peroxy radical generating unit 20 when peroxy radical calibration, and the gas phase / liquid phase separation unit 40 when measuring peroxy radicals. The carrier solution is transported to one side, and the luminescent solution that forms the fluorescent emitter by chemical reaction is transported to one side of the cochlear reactor 50.

In this case, the solution carrier 10 is preferably a PTFE (Polytetrafluoroethylene) connection line and pump suitable for various chemical reactions, it is preferable to use the MCLA solution as the light emitting solution.

The peroxy radical generating unit 20 generates peroxy radicals by photolyzing an aqueous solution whose pH is appropriately introduced from the outside to calibrate the peroxy radicals.

At this time, the peroxy radical generating unit 10 generates a peroxy radical through the photochemical reaction of the aqueous solution introduced through the injection port 1 through the photocatalyst as shown in FIG. The coil reactor 11 for discharging the liquid sample of radicals to the outside through the discharge port 2, the ultraviolet lamp 12 for irradiating the ultraviolet rays to the coil reactor 11, and the heat generated therein is discharged to the outside It consists of a cooling fan 13 which maintains a constant temperature.

At this time, titanium oxide (TiO ₂ ) of the nano-sized particles are doped in the coil reactor 11, and the titanium oxide is very firmly attached, so that it can be reused for a long time in an aqueous solution.

For example, in the peroxy radical generating unit 10, titanium oxide doped in the coil reactor 11 and ultraviolet light react with each other to form a cation hole (h ⁺ ) and an anion electron (e ⁻ ) as shown in Equation 1 below. The electron of this anion combines with oxygen contained in the liquid sample introduced from the outside as shown in Equation 2 to form peroxy radicals. The peroxy radical (HO ₂ ·) forms an acid-base equilibrium (K _{HO 2} = 4.88) in an aqueous solution as in Equation 3.

[Equation 1] TiO ₂ + UV → h ^{+ +} e ^-

[Equation _{^{2] e - + O 2 →}} O 2 - ·

HO₂· [Equation ^{_{3] O 2 - · + H}} +

HO ₂ ·

At this time, the peroxy radical concentration may be adjusted by Equations 4 and 5 in consideration of the mixing of the aqueous solution.

[Formula 4] HO ₂ · + HO ₂ · → H ₂ O ₂ + O ₂

[Equation _{5] HO 2 · + O 2} - · + H + → H 2 O 2 + O 2

In particular, O ₂ ^- · against O ₂ ^- · reaction between HO ₂ ·, but rarely for HO ₂ · and HO ₂ · for O ₂ ^- · reaction between their place very quickly. Expressed in terms of reaction rate, it is the same as Equation 6.

At this time, the integrated solution of Equation 6 becomes Equation 7.

When, in formula _{7 [HO 2 · / O 2} - ·] _t the concentration of the initial concentration of ^{_{[HO 2 · / O 2 -}} ·] If the half-life (t _1/2) is half of the ₀ formula 7 formula 8 Becomes

Where k _obs is the rate constant and k _obs is

Where k ₆ = 7.61 × 10 ⁵ [M ⁻¹ S ⁻¹ ], k7 = 8.5 × 10 ⁷ [M ⁻¹ S ⁻¹ ], K _{HO 2} = 4.88

Therefore, if only the half-life value is known from Equation 8, the concentration of [HO ₂ · / O ₂ ⁻ ·] ₀ can be directly obtained.

The knotted reactor 30 passes the peroxy radicals generated by photolysis of the peroxy radical generating unit 20 through knotted tubes having different lengths in order to calculate a half-life for detecting the initial concentration of peroxy radicals. To change the concentration of peroxy radicals.

At this time, the knot-type reactor 30 forms a tube of a predetermined length through which the peroxy radicals fished from the peroxy radical generating unit 20 can pass in the form of a knot 31 as shown in FIG. 3, The overall length of this knot is formed in various lengths, such as 1 m, 2 m, 3 m, 4 m and the like.

For example, if the knot type reactor 30 having a different length between the knot type reactor 30 and the snail type reactor 50 is attached and detached and passed through the peroxy radical generated in the peroxy radical generating unit 20, the knot is knotted. As the length of the reactor 30 changes, the concentration of peroxy radicals changes. That is, the peroxy radicals decrease in concentration of peroxy radicals as the length of the knot-type reactor 30 increases, while the concentration of peroxy radicals increases in shorter lengths.

The gas phase / liquid phase separation unit 40 separates the atmospheric or liquid sample introduced from the outside mixed with the transport solution carried in the solution transport unit 10 into the gas phase and the liquid phase, and releases the gas phase to the outside, and only the liquid phase is a snail type. Flow out into the reactor 50.

For example, when the measurement object flowing into the gas phase / liquid phase separation unit 40 is an atmospheric sample, in order to measure peroxy radicals contained in the atmospheric sample, the atmospheric sample must be dissolved in a transport solution and changed to a liquid phase. At this time, the gas phase insoluble in the transport solution will release the outside. Of course, when the measurement target is a liquid sample, the liquid sample is immediately discharged into the snail reactor 50 without the need to separate the gas phase and the liquid phase.

The cochlear reactor 50 reacts with the solution passed through the knotted reactor 30 or the gas phase / liquid phase separation unit 40 and the MCLA solution carried through the solution delivery unit 10 to form a fluorescent emitter.

In this case, the cochlear reactor 50 has a first inlet 51 and a solution carrying part into which the solution discharged from the knotted reactor 30 or the gas phase / liquid phase separation part 40 is introduced as shown in FIG. 4. 10) the second inlet 52 into which the MCLA solution is carried is separated, and the cochlear tube through which the transport solution and the MCLA solution introduced through the first and second inlets 51 and 52 are mixed. 53 and the solution passing through the cochlea 53 are formed by a discharge port 54 which is transported by the solution carrying section 10 and discharged to the outside.

For example, when the transport solution and the MCLA solution are mixed in the cochlear reactor 53 of the cochlear reactor 50, the peroxy radicals contained in the transport solution and the MCLA solution react to form an intermediate as shown in Equation 10, and the intermediate Equation 11 emits a characteristic fluorescent light.

[Equation 10] HO ₂ · or ^{_{O 2 - · + MCLA → MCLA}} *

[Formula 11] MCLA * → Fluorescence

The emission amount detection unit 60 measures the fluorescent emission light formed in the snail reactor 50 and converts and outputs an electric signal corresponding thereto.

For example, in the fluorescent emission detected by the emission amount detecting unit 60, a difference in fluorescence intensity occurs depending on the length of the knot-type reactor 30. That is, the longer the length of the knot-type reactor 30, the concentration of the peroxy radicals decreases, and the size of the signal decreases, while the shorter the length, the concentration of the peroxy radicals increases, and the size of the signal becomes larger. .

The signal analysis unit 70 detects the concentration of the initial peroxy radical by analyzing the electrical signal output through the emission amount detecting unit 60, and calibrates by setting the fluorescence intensity of the initially detected fluorescent emitter to the initial peroxy radical concentration. Then, the concentration of the peroxy radicals included in the measurement object is detected by comparing the fluorescence intensity of the measurement object with the fluorescence intensity calibrated.

For example, the signal analysis unit 70 may calculate a signal ratio as shown in Equation 12 by analyzing the electrical signal output from the light emission amount detecting unit 60. The signal ratio may be set as the Y-axis and knotted. The length of the type reactor may be represented as shown in FIG. 5 according to the reaction pH conditions.

Here, A ₀ is the intensity of the signal represented by the length of the knot-type reactor 30 at 0m, An is the intensity of the signal represented by the length of the knotted reactor 30 1m, 2m, 3m, 4m.

In this case, the signal ratio may be represented by a first-order equation as shown in Equation 13 using slope and intercept shown in FIG. 5.

Signal ratio = slope × length of knot reactor + intercept

Therefore, the signal analysis unit 70 may calculate the slope and the intercept of the equation 13 by analyzing the signal output from the light emission amount detection unit 60.

In addition, D _{t1 / 2} (1/2 of the reactor length) can be obtained from Equation 13 derived from the signal ratio at half-life and the initial signal strength and half-life signal strength measured at half-life as shown in Equation 14.

Therefore, D _{t1 / 2} values from the flow rate in the knot-type reactor, and to obtain the t _{1/2, 1/2} t Substituting this in equation (8) the initial concentration of peroxy radicals, that is, ^{_{[HO 2 · / O 2 -}} · ] ₀ can be calculated.

At this time, the signal analysis unit 70 is the fluorescence intensity of the fluorescent emitter detected initially when the calculated initial peroxy radical concentration, that is, the fluorescence intensity of the fluorescent emitter detected when the length of the knot-type reactor 30 is 0m (A ₀ ) Set to correspond to. For example, if the signal strength of A ₀ is detected in any unit of 10, the initial peroxy radical concentration is set to a value corresponding to any unit value of signal strength 10.

Thereafter, the signal analysis unit 70 calculates the concentration of the peroxy radicals included in the measurement target by comparing the fluorescence intensity of the measurement target output to the emission amount detection unit 60 with the type and intensity of the set initial peroxy radicals. do.

The plurality of valves V1 to V4 are opened and closed to calibrate the peroxy radicals or to measure the peroxy radicals included in the measurement object. For example, when calibrating peroxy radicals, the first and second valves V1 and V2 are opened, the third and fourth valves V3 and V4 are closed, and the peroxy included in the measurement object. In the radical measurement, the third and fourth valves V3 and V4 are opened and the first and second valves V1 and V2 are closed. Therefore, in the peroxy radical calibration, the solution transport unit 10, the peroxy radical generating unit 20, the knot type reactor 30, the snail type reactor 50, the emission amount detecting unit 60, the signal analysis unit 70 When the peroxy radicals are measured, the solution transport unit 10, the gas phase / liquid phase separation unit 40, the snail reactor 50, the emission amount detection unit 60, the signal analysis unit 70, etc. Will react.

When explaining the peroxy radical measuring method of the present invention made as described above with reference to FIG.

First, in order to calibrate the peroxy radicals, the first valve V1 and the second valve V2 are opened, and the pH is properly adjusted while the third valve V3 and the fourth valve V4 are closed. The prepared aqueous solution is added to the peroxy radical generating unit 20 (S10).

Subsequently, the peroxy radical generating unit 20 mixes the transport solution carried in the solution transport unit 10 and the air sample or water sample introduced therein, and the mixed aqueous solution is expressed as in Equations 1, 2, and 3 below. After photolysis to produce a peroxy radical, this aqueous solution is introduced into the knot-type reactor (30) (S11).

At this time, the knot-type reactor 30 is different in the total length of the knots, such as 0m, 1m, 2m, 3m, 4m, such that the total length is passed in the knot-type reactor 30, the total length is generated in the aqueous solution The concentration of the peroxy radicals is changed and introduced into the snail reactor 50 (S12).

Subsequently, the snail reactor 50 reacts with the incoming aqueous solution and the MCLA solution carried in the solution carrier 10 to generate a fluorescent emitter as shown in Equations 10 and 11, and the fluorescence intensity of the fluorescent emitter is a knotted reactor. According to the change in the length of 30, a difference in fluorescence intensity occurs. That is, when the concentration of peroxy radicals decreases, the fluorescence intensity decreases, and when the concentration of peroxy radicals increases, the fluorescence intensity increases (S13).

At this time, the light emission amount detection unit 60 detects the light emission intensity of the fluorescent light emitting body generated in the cochlear reactor 50 and outputs an electrical signal corresponding thereto to the signal analysis unit 70 (S14).

Subsequently, the signal analysis unit 70 analyzes the signal detected according to the change in the length of the knotted reactor 30 and calculates the signal ratio as shown in Equation 12. Then, the signal ratio according to the reaction pH condition and the length of the knotted reactor are shown. A graph of X and Y axes is generated as shown in FIG. 5 (S15).

In this case, the signal ratio may be calculated by the first equation of the slope and the intercept as in Equation 13, and the slope and the intercept may be calculated in the first equation (S16). This can be calculated slope and intercept of the D _{t1 / 2} is substituted in equation 15, the half-life can be obtained from the D _{t1 / 2} value and the flow rate of the reactor length (S17). Substituting the half-life calculated in this way into Equation 8 yields an initial peroxy radical concentration (S18).

Subsequently, calibration is completed by setting the fluorescence intensity of the fluorescent emitter initially detected, that is, the fluorescence intensity measured at 0 m of the knotted rebounder to the initial peroxy radical concentration (S19).

Thereafter, the third valve V3 and the fourth valve V4 are opened to measure the peroxy radical included in the atmospheric sample or the liquid sample to be measured, and the first valve V1 and the second valve ( In the state in which V2) is closed, the measurement solution is introduced into the gas phase / liquid separation unit 40 (S20).

At this time, the gas phase / liquid phase separation unit 40 mixes the incoming measurement solution with the transport solution carried in the solution transport unit 10, then discharges the gas phase to the outside, and introduces the liquid phase into the snail reactor 50. . Subsequently, the cochlear reactor 50 reacts with the incoming measurement solution and the MCLA solution carried by the solution transport unit 10 to generate a fluorescent light emitting body as shown in Equations 10 and 11 (S21).

At this time, the light emission amount detection unit 60 detects the light emission intensity of the fluorescent light emitting body generated in the snail-shaped reactor 50 and outputs an electrical signal corresponding thereto to the signal analysis unit 70 (S22).

At this time, the signal analysis unit 70 compares the fluorescence intensity of the set initial peroxy radical concentration with the fluorescence intensity of the detected measurement target to detect the peroxy radical concentration included in the measurement target (S23).

So far I looked at the center of the preferred embodiment for the present invention. Those skilled in the art will appreciate that the present invention can be implemented in a modified form without departing from the essential features of the present invention. Therefore, the disclosed embodiments should be considered in descriptive sense only and not for purposes of limitation. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the scope will be construed as being included in the present invention.

As described above, the present invention generates peroxy radicals by photolyzing an aqueous solution having a pH adjusted appropriately, calculating a half-life by measuring a change in concentration of the peroxy radicals, and then using the half-life of the peroxy radicals. The initial concentration is detected and calibrated. Based on the calibrated initial concentration, the peroxy radicals contained in the air sample or the liquid sample can be measured to not only reduce the measurement cost but also accurately detect the small amount of peroxy radicals. It can be measured.

Claims (6)

A solution transport unit for separately transporting various transport solutions necessary for a chemical reaction and a luminescent solution reacting with a peroxy radical to form a light emitter; A peroxy radical generating unit for generating peroxy radicals by photolysing an aqueous pH-adjusted aqueous solution to calibrate peroxy radicals; A knotted reactor for varying the concentration of the peroxy radicals by passing the peroxy radicals through knotted tubes of different lengths to produce a half-life for detecting the concentration of the initial peroxy radicals; A gas phase / liquid phase separation unit that separates the air sample or liquid sample mixed with the transport solution carried in the solution transport unit into the gas phase and the liquid phase and discharges the gas phase to the outside; A snail reactor for reacting a solution passing through a knotted reactor or gas phase / liquid phase separation unit with a luminescent solution carried through the solution carrier to form a fluorescent light emitting body; A light emission amount detector for measuring a fluorescence intensity of the fluorescent light emitting body formed in the snail reactor and outputting an electric signal; Analyze the electrical signal output through the light emission detection unit to detect the concentration of the initial peroxy radicals, set the fluorescence intensity of the initially detected fluorescent emitters to the initial peroxy radical concentration and calibrate, and then the fluorescence intensity and calibration of the measurement target A signal analysis unit for detecting a concentration of peroxy radicals included in the measurement target compared to the fluorescence intensity which has been converted; Peroxy radical measurement, characterized in that consisting of a plurality of valves that are opened and closed to measure the concentration of the peroxy radicals produced by photolysis of the aqueous solution or to measure the concentration of the peroxy radicals contained in the air sample or liquid sample Device. The coil reactor of claim 1, wherein the peroxy radical generating unit generates peroxy radicals by photolysing the aqueous solution introduced through the inlet, and then discharges the atmospheric or liquid sample to the outside through the outlet with the peroxy radicals thus generated. And an ultraviolet lamp for irradiating ultraviolet rays to the coil reactor, and a cooling fan for discharging heat generated inside to the outside to maintain a constant temperature. 3. The peroxy radical measuring device according to claim 2, wherein the coil reactor is doped with titanium oxide (TiO ₂ ) of nano-sized particles. According to claim 1, The knot-type reactor is a peroxy radical measuring device, characterized in that a certain length of pipe to pass through the knot-shaped, the total length of the knot is formed in a plurality of different peroxy radical measuring apparatus . 2. The cochlear reactor according to claim 1, wherein the cochlear reactor is divided into a first inlet through which a solution discharged from a knotted reactor or a gas phase / liquid separator is introduced, and a second inlet through which a light emitting solution carried by the solution carrier is introduced. And a cochlear tube in which the transport solution and the luminescent solution introduced through the first and second inlet ports are mixed and passed through, and the outlet port through which the solution passed through the cochlea is carried by the solution transport unit and discharged to the outside. Roxy radical measuring device. Photolyzing the pH-adjusted aqueous solution introduced from the outside to calibrate the peroxy radicals to generate peroxy radicals; Adjusting the length of the knotted reactor to vary the concentration of the peroxy radicals produced above; Reacting the peroxy radicals with the luminescent solution to form a fluorescent emitter, and detecting a change in concentration of the peroxy radical by detecting the fluorescence intensity of the fluorescent emitter; Calculating a linear equation by comparing the detected fluorescence intensity with a signal ratio according to pH conditions and a length of the knotted reactor as X and Y axes, respectively; Calculating a half-life using the slopes and intercepts calculated in the linear equations; Detecting the initial peroxy radical concentration using the calculated half-life; Setting the fluorescence intensity of the initially detected fluorescent emitter to an initial peroxy radical concentration to complete calibration; Reacting the object to be measured with the luminescent solution to form a fluorescent emitter, and detecting the fluorescence intensity of the fluorescent emitter; And detecting the peroxy radical concentration by comparing the fluorescence intensity of the set initial peroxy radical concentration with the fluorescence intensity of the measurement target.

Images