KR20070004150A - Nerium genus plant extract for preventing growth of bacterium in cooling water and method for preventing growth of bacterium in cooling water using the same - Google Patents

Abstract

The extract of Nerium genus plants for inhibiting growth of bacteria in cooling water and a method for inhibiting growth of bacteria in cooling water by using the same extract are provided to reduce the costs for sterilizing the cooling water by using inexpensive material, and manage the cooling water without environmental pollution. The extract of Nerium genus plants for inhibiting growth of bacteria in cooling water contains oleandroside and nerioside as effective components, wherein the extract of Nerium genus plants is obtained by using hydrogen peroxide; the Nerium genus plants include Nerium indicum, Nerium indicum for. plenum, Neriumindicum for. lutescens, Nerium indicum for. leucanthum, or Nerium oleander; and the concentration of Nerium genus plants treated to cooling water is 100-200ppm. The method for inhibiting growth of bacteria in cooling water comprises the steps of: drying the Nerium genus plants; pulverizing the dried Nerium genus plants; adding hydrogen peroxide into the pulverized Nerium genus plants, stirring the mixture, and storing it for 3-10 days; adding water into the mixture, and mixing and filtering the mixture to prepare the extract of Nerium genus plants; and treating cooling water with 100ppm or more of the Nerium genus plant extract.

Description

Nerium Genus Plant Extract for preventing growth of bacterium in cooling water and method for preventing growth of bacterium in cooling water using the same}

Figure 1 shows the effect of inhibiting the growth of microorganisms in the cooling water according to the time when dilution applied to 100 ~ 200ppm concentration range of the oleander plant extract.

Figure 2 shows the effect of killing bacteria after leaving the oleander plant extract in cooling water (concentration: 0.1, 0.2, 0.5, 1.0ml / l) for 1 day.

The present invention relates to a plant extract for suppressing bacterial growth in cooling water and a method for inhibiting bacterial growth in cooling water using the same, and more particularly, to an environment-friendly and economical process for controlling bacterial growth in cooling water. It relates to a method for inhibiting bacterial growth.

Hot water cooling systems are relatively slow in microbial growth because of the relatively low concentrations of nutrients essential for microbial growth, while open-circulation cooling systems introduce microbes in the air and concentrate the nutrients in the supplemental water through evaporation. Faster microbial growth. In such open-circulation cooling systems, organic and inorganic nutrients, appropriate temperature and pH ranges, and continuous air supply provide an ideal environment for microbial growth, resulting in the emergence of various microbial populations. If it does not inhibit the microorganisms on the surface slime (slime) is formed. The slime obstacle can be clearly seen that slime formed by the deposition or deposition of pollutants formed by mixing bacteria, minerals, and dust by using nutrient sources dissolved in water. Wood-destroying microorganisms penetrate the wood of the cooling tower to eat wood, destroying the structure, and microbial activity in the bottom of the sediment or in the slime increases the rate of corrosion and corrodes the heat transfer surface. The slime disturbance phenomenon in the cooling system is as follows.

[Table 1] Slime Phenomenon in Cooling System

Type of slime obstacle Contents Slime Attached Fouling Obstacles caused by the addition of a mixture of microorganisms and earth and sand to the solid surface due to the action of the adhesive material produced by the microorganism Slime deposit fouling Caused by pollutants formed by sedimentation and sedimentation at low flow rates in water

In the conventional cooling water system, microbicides used as a method for preventing microorganisms include oxidizing biocides and nonoxidizing biocides. In 1999, a study examined the effects of oxidizing and non-oxidizing fungicides in circulating cooling systems. Analytical agents measured the concentration in the coolant. For all the drugs reviewed, toxicological tests were performed to assess the impact on surface water. When cooling water was released directly into surface water in a circulating cooling system, the use of non-oxidizing fungicides was observed to have a significant adverse effect on the surface water quality. Predicted Environmental Concentration (PEC) / Predicted No Effect Concentration (PNEC) ratios based on toxicity studies using hypochlorite were found to be 3 (continuous injection) and 33 (impact injection), which were found to be non-oxidative (DBNPA). It became. Another study found that the use of isothiazolone (1,2-benzisothiazolin-3-on, 2-methyl-4-isothiazolin-3-on) as a coolant additive could not block the potential risk to surface water. .

Oxidizing biocide has a strong oxidizing power to oxidize microbial cell proteins to sterilize. Chlorine (or a combination of chlorine and bromine) and monochloramine are mainly used. Chlorine and bromine are strong oxidants (acutely toxic) with short half-lives, but they come into contact with halogens to form halogenated by-products. Other oxidizing fungicides include ozone, ultraviolet light, hydrogen peroxide and peracetic acid. Ozone and ultraviolet light require pretreatment of supplemented water or additives, which are less harmful than halogenated fungicides, but are expensive and cannot be applied in all situations.

Chlorine sterilizers most commonly used as cooling water disinfectants include chlorine gas, calcium hypochlorite, sodium hypochlorite, and the like, although there are differences in form, there is no difference in the principle of action of these chlorine disinfectants. Chlorine fungicides produce hypochlorous acid (HOCl) and chlorine ions (OCl-) in water, as shown in Equations 1 and 2 below, and the bactericidal power of HOCl is about 50 to 100 times greater than that of OCl-. have.

[Equation 1]

Chlorination: Cl ₂ + H ₂ O → HCl + HOCl

[Equation 2]

HOCl → H + OCl-

In the chlorine sterilization method, the concentrations of HOCl and OCl- change with pH, and HOCl has better sterilization ability than OCl-. As the pH increases, the ratio of OCl- to HOCl increases, which reduces the disinfectant effect of chlorine disinfectant. Therefore, when the pH of the cooling water is high, various measures to increase the income effect are necessary. Since chlorine is corrosive to the metal materials of the cooling system, the residual chlorine should be 1 mg / L or less and the treatment time should be as short as possible. Therefore, even if chlorine treatment is not possible, it is impossible to completely prevent the growth of microorganisms, so it is effective to regularly add a slime inhibitor which does not have a problem in corrosion other than chlorine. In addition, in the chlorine sterilization method, the sterilization ability may be greatly reduced by reaction with ammonia or amine chemicals present in the cooling water. The chlorinated amine, the reaction product, has very poor sterilization ability.

Nonoxidizing biocide exerts bactericidal activity by inhibiting the metabolism of microorganisms. Non-oxidizing fungicides include isothiazolones, methylene bisthiocyanate, glutaraldehyde, and quaternary ammonium. It has the characteristic that it is. Most of these substances are acutely toxic and most are not readily biodegradable, although some may be hydrolyzed or degraded by other mechanisms.

In view of this, fungicides sold are often formulated with one or more active ingredients. Microbial growth can be limited by appropriately mixing one or more active ingredients to increase sterilization range. The development of an effective microbial treatment program is only possible with a good understanding of how the product works, its treatment system and its environmental impact. All of these factors play an important role in the selection of microbial control programs that are safe, effective and economical for the environment. Most of them are used in combination with a surfactant as a copolymer and have poor biodegradability.

In particular, microbial fouling in cooling systems that use fresh water as industrial water occurs when microorganisms multiply on the surface. Fouling problems usually occur more extensively than in open-circulation cooling systems. Microorganisms that form slime deposits in cooling systems are typically microorganisms that live in soil, water and air. These microorganisms are introduced into the system through the replenishment water, and when the replenishment is clean water, the number is small, but when the turbidity of the sewage level is turbid. In addition, as air is sucked into the cooling tower, a significant amount of microorganisms may be contained in the cooling water. In open-circulation cooling systems, there are many obstacles of the genus Zogloea sp., Followed by algae, filamentous fungi and filamentous fungi. In addition, even if the slime mainly composed of aerobic bacteria, the lower part is anaerobic and corrosion may occur due to anaerobic bacteria such as yellow reducing bacteria.

Therefore, in order to solve this problem, it is required to develop eco-friendly and economical treatment methods by controlling microorganisms with materials extracted from biological materials that do not cause secondary pollution.

On the other hand, oleander is a very toxic plant and is known to be lethal even if the animal ingests a small amount of 0.005% of the animal's weight (Turner and Szczawinski, 1999). In animals, the toxic signs of oleander plants are nausea, severe vomiting, stomach cramps, dizziness, slow pulse, irregular heartbeat, dilated pupils, diarrhea, drowsiness, lethargy, paralysis, and the strength of the signs depends on the level of intake. It is reported to appear differently.

Therefore, an object of the present invention is to use economical new biological material without the use of expensive microbial treatment agent or pesticide to control microorganisms in cooling system water using fresh water, and to save economic cost of medicine and environmentally friendly cooling water management. It is to provide a oleander plant extract for inhibiting bacterial growth in cooling water having bactericidal activity.

Another object of the present invention is to provide a method for inhibiting bacterial growth in cooling water using the oleander and plant extract for inhibiting bacterial growth in the cooling water.

According to the first aspect of the present invention, there is provided an oleander plant extract for inhibiting bacterial growth in cooling water, which contains at least one component of oleandroside and nerioside as an active ingredient and has anti-bacterial activity. .

According to a second aspect of the invention, the step of drying the oleander and plants; Grinding the dried oleander and plants; Adding hydrogen peroxide to the ground plant in an amount of 5-20 times by weight, stirring and standing for 3-10 days; Adding water in an amount of 2-6 times by weight, mixing and filtering to obtain an oleander plant extract; And it provides a method for inhibiting bacterial growth in the cooling water using the oleander plant extract comprising the step of treating the extract at a concentration of 100-200ppm in the cooling system water.

Hereinafter, the present invention will be described in detail.

The present invention relates to a oleander plant extract effective in inhibiting the growth of bacteria in the cooling water and a method for inhibiting bacterial growth in the cooling water using the same, and cardioactive extracted from each organ including flowers, leaves, branches and roots of oleander plants. Oleander plant extracts containing glycoside components olendroside and nerioside as active ingredients effectively inhibit the growth of bacteria in the cooling water. Preferably at least one component of the oledroside and nerioside components is included in the extract at least 0.2% by weight or more, more preferably 0.2-0.4% by weight.

 The growth of bacteria in cooling water is considered to be inhibited by the action of cardioactive glycosides, in particular oledroside and nerioside, which act as a main component of oleanders and plant extracts to block the metabolism of life. The oleander plant extract is a common method of extracting active ingredients from plants and is extracted from each organ of the oleander plant including flowers, leaves, branches, stems, and roots.

The oleander plant extract may be used any common extraction method using water and alcohol, it is particularly preferably extracted using hydrogen peroxide. The extraction method includes the olendroside and nerioside components, which are cardioactive glycoside components, which are antimicrobial active ingredients, in each organ of plants such as flowers, leaves, branches, stems, and roots. Naturally dry the organs of oleanders and plants such as stems and roots. However, the moisture content contained in the naturally dried oleander and the plant may be different, and it is also dried at about 65-70 ° C. for 48-72 hours to remove residual moisture. After drying to remove moisture, pulverized into a powder of 2mm or less, distilled water was added to the pulverized plant in an amount of 10-20 times, stirred for 1-3 days, left for 7 days, filtered and filtered Is obtained as a stock solution (extract). Extraction method when using hydrogen peroxide, for example, drying the oleander and plants; Grinding the dried oleander and plants; Adding hydrogen peroxide to the ground plant in an amount of 5-20 times by weight, stirring and standing for 3-10 days; And adding water in an amount of 2-6 times by weight, mixing and filtering. This filtered solution is used as a stock solution (extract solution), which is diluted in an appropriate ratio and applied in cooling water to be removed.

Although not limited to this, the oleander extract may be applied by any method such as spraying, spraying into the cooling water to be removed.

The oleander extract may be applied to any application for suppressing the growth of bacteria in the cooling water, but is not limited thereto, and may be particularly preferably used to suppress the growth of bacteria in a cooling system using fresh water as industrial water.

The oleander plant extract is applied to dilute the concentration of 100ppm or more and preferably 100-200ppm in the cooling water when applied, and if less than 100ppm, the effect of inhibiting bacterial growth in the cooling water is reduced, and the cost is more than 200ppm. This will increase.

The oleander is not limited to this, for example, Nerium indicum , Nerium indicum for. plenum ), Yellow Oleander indicum for. lutescens ), oleander ( Nerium) indicum for. leucanthum ) and Nerium oleander .

Microorganisms that can inhibit the growth by applying the oleander plant extract is not limited thereto, but the genus Salinibacterium ( Salinibacterium) sp . ), Aurantimonas sp . Spingomonas sp . ), Porphyrobacter tepidarius , Chryseobacterium sp . ), Hydrogenphaga sp. , Aeromonas hydrophila hydrophila ), Bacillus megaterium , Micrococcus luteus), Pseudomonas Oh Rouge in labor (Pseudomonas aeruginosa), Bacillus Pew milreoseu (Bacillus pumilus), brilliants Pseudomonas Bundy Bezier Temecula less (Brevundimonas vesicularis), hydroxyl halogen wave in (Hydrogenphaga sp . ), Staphylococcus warner , Roseomonas mucosa ), Staphylococcus epidermidis , Acidovorax delafieldii , Aeromicrobium sp . ), Microbacterium oxydans , Pedobacter sp . ) Or variants thereof.

Hereinafter, the present invention will be described in detail through examples. The following examples illustrate the invention but do not limit the invention.

<Example 1>

Preparation of the extract was carried out by the following procedure. Each organ (flower, leaf, branch, stem, root) of oleander plants was collected from native plants, completely dried in the shade, and then powdered to 2 mm or less using a grinder. Oleander plant extract is a common method of extracting active ingredients from plants and is extracted from the organs of oleander plants including leaves. Any general extraction method using water, alcohol and hydrogen peroxide can be used, but in the case of hydrogen peroxide, it is the best in terms of the extraction of the active ingredient and its effect. 에서는 In the present invention, it was extracted with hydrogen peroxide as follows. First, naturally dry the organs of oleander plants including leaves. However, the moisture content contained in the naturally dried oleander and the plant may be different, and it is also dried at about 65-70 ° C. for 48-72 hours to remove residual moisture. After drying to remove moisture, it was pulverized into a powder of 2mm or less, 100g of oleander and plant powder in 1000ml of hydrogen peroxide and stirred for 24 hours, and left for 7 days, and then put in 4000ml of water to make 5000ml and filtered (plant material) : Hydrogen peroxide: water = 1: 10: 40). This filtered solution was used as a stock solution (extract), which was diluted to an appropriate ratio and applied to the microorganisms in the indirect cooling water to be removed.

Characteristics and identification results of purely isolated dominant bacteria are shown in Table 1. These were identified as 14 Gram-negative bacteria and 10 Gram-positive bacteria. Among these bacteria in the Pseudomonas Oh dihydro pillar (Aeromonas hydrophila), or Staphylococcus epidermidis was the detection of pathogenic bacteria such as (Staphylococcus epidermidis) were isolated seven new bacteria. In general, they are mesophilic bacteria and prefer to be neutral or alkaline. In addition, as a bacterium that requires more than 3% NaCl, it was found that it is slightly affected by sea water.

[Table 1] 특성 Characteristics and Identification of Bacteria Isolated from Cooling Water

Strain No. Sympathy New species Growth at NaCl (%) Growth at pH Growth at Temp. ( ^O C) 3 5 7 10 4 7 10 4 25 40 One Salinibacterium sp . nov. v _ _ _ _ _ + + - + + 5 Aurantimonas sp . nov. v + + _ _ - + + - + -/ + 7 Spingomonas usincola + _ _ _ - + + - + +/- 8 Porphyrobacter tepidarius _ _ _ _ - + + - + + 9 Chryseobacterium sp . nov v _ _ _ _ - + - - + - 11 Hydrogenphaga sp . + + + + - + + - + + 12 Aeromonas hydrophila + _ _ _ - + + - + + 13 Bacillus megaterium + + + + - + + - + + 14 Micrococcus luteus + + + + - + + - + + 17 Porphyrobacter tepidarius _ _ _ _ _ + + - + + 18 Pseudomonas  aeruginosa + -/ + _ _ - + + - + + 19 Spingomonas sp . nov. v _ _ _ _ - + _ - + - 20 Bacillus pumilus + + + + - + + - + + 21 Brevundimonas  vesicularis + +/- _ _ - + + +/- + - 23 Hydrogenphaga sp . _ _ _ _ - + + - + - 24 Spingomonas usincola -/ + _ _ _ _ + + - + + 27 Staphylococcus warner + + + + - + + - + + 28 Roseomonas mucosa + _ _ _ - + + - + + 31 Staphylococcus epidermidis + + + + - + + - + + 32 Acidovorax delafieldii _ _ _ _ _ + + - + +/- 35 Aeromicrobium sp . nov. v + + -/ + - - + - - + + 37 Spingomonas sp . nov. v + _ _ _ - + + - + -/ + 38 Microbacterium  oxydans + + _ _ - + + +/- + +/- 39 Pedobacter sp . nov. v - _ _ _ - + - - + -

<Example 2>

Water quality samples were collected from indirect cooling system water operated in the oxygen plant of POSCO Gwangyang Steel Works in Jeonnam. After culturing for 7 days at 20 ℃ colony producing bacteria (CFU) was measured. For these bacteria, extracts were added to the liquid medium to make the final concentration untreated, 0.1 and 0.2ml / l, and then inoculated with purely isolated bacteria to measure absorbance every 2 hours while shaking culture (25 ℃, 180rpm) (600nm). ) Growth of bacteria was measured. In addition, the extract was added to cooling water (no treatment, 0.1, 0.2, 0.5, 1 ml / l), and then left for 1 day, after which the bacterial count was measured.

As shown in Fig. 1, 100-200ppm concentration of the oleander plant extract has the effect of inhibiting the proliferation of microorganisms in the concentration and time zone by using the oleander plant extract including the olendroside and the nelioside component, which are cardioactive glycoside components. When applied in a dilution range, the isolated and identified microorganisms were 100% removed. The use of liquid media inhibited the growth of bacteria from the lower concentration of 0.1 ml / l. That is, in the liquid state, it is easy to diffuse the components of the extract and the growth rate of the bacteria is fast, so the effect of the material appears at a low concentration.

In addition, as shown in FIG. 2, the effect of bacterial killing in the cooling water of the extract was measured after adding the stock extract of the plant extract to the cooling water (concentration: 0.1, 0.2, 0.5, 1.0ml / l) for 1 day and then measuring the density of bacteria. As a result, it was confirmed that the density of bacteria was sharply lowered at a concentration of 0.1 ml / l or more similarly to the result of using the liquid medium.

Therefore, by the method of the present invention, it is possible to effectively control the microorganisms causing biofouling in the cooling water in a short time. In addition, since the oleander extract of the present invention is biodegradable, it is considered that secondary contamination problems such as concentration of the hardly degradable substances exhibited during the disaster prevention by conventional medicine treatment and soil contamination by this are solved.

According to the present invention, since no expensive microbial treatment agent or pesticide is used to control the microorganisms in the cooling system water using fresh water, it is possible to expect the economic cost reduction effect of the medicine and environmentally friendly cooling water management.

Claims (11)

Oleander plant extract for inhibiting bacterial growth in cooling water having at least one component of oleandroside and nerioside as an active ingredient and having anti-bacterial activity. According to claim 1, wherein the oleander plant extract is oleanderaceae plant extract for inhibiting bacterial growth in the cooling water, characterized in that extracted with hydrogen peroxide. According to claim 2, The extraction method using hydrogen peroxide comprises the steps of drying the oleander and plants; Grinding the dried oleander and plants; Adding hydrogen peroxide to the ground plant in an amount of 5-20 times by weight, stirring and standing for 3-10 days; And oleander and plant extract for inhibiting bacterial growth in the cooling water, characterized in that consisting of the step of adding water in an amount of 2-6 times by weight and mixing and filtering. The oleander plant extract for inhibiting bacterial growth in cooling water according to claim 3, wherein the oleander plant is selected from at least one of flowers, leaves, branches and roots of the oleander plant. According to claim 1, wherein the oleander plant is Oleander ( Nerium) indicum), mancheop oleander (Nerium indicum for. plenum), yellow oleander (Nerium indicum for. lutescens), white oleander (Nerium indicum for. leucanthum) and up meander oleander (Nerium oleander) is selected from the group characterized in that the plant extract apocynaceae for suppressing bacterial growth in cooling water constituting a. According to claim 1, wherein the bacteria are Salinibacterium genus ( Salinibacterium) sp . ), Aurantimonas sp . , Spingomonas sp . ), Porphyrobacter tepidarius , Chryseobacterium sp . ), Hydrogenphaga sp . , Aeromonas hydrophila hydrophila ), Bacillus megaterium , Micrococcus luteus), Pseudomonas ah Rouge Labor (Pseudomonas aeruginosa), Bacillus Pew milreoseu (Bacillus pumilus), Brenna Bundy Monastir Beziers Temecula lease (Brevundimonas vesicularis ), Hydrogenphaga sp . ), Staphylococcus warner , Roseomonas mucosa ), Staphylococcus epidermidis , Acidovorax delafieldii , Aeromicrobium sp . ), Microbacterium oxydans or Pedobacter sp . Oleander plant extract for inhibiting bacterial growth in cooling water, characterized in that it comprises a). According to claim 1, wherein the extract is oleanderaceae plant extract for inhibiting bacterial growth in the cooling water, characterized in that the treatment is treated at a concentration of at least 100ppm or more in the cooling water. According to claim 7, wherein the extract is oleanderaceae plant extract for inhibiting bacterial growth in the cooling water, characterized in that the treatment is treated at a concentration of 100-200ppm in the cooling water. Drying the oleander and plants; Grinding the dried oleander and plants; Adding hydrogen peroxide to the ground plant in an amount of 5-20 times by weight, stirring and standing for 3-10 days; Adding water in an amount of 2-6 times by weight, mixing and filtering to obtain an oleander plant extract; And Treating the extract to a concentration of at least 100 ppm or more in cooling water; Method of inhibiting bacterial growth in cooling water using oleander plant extract comprising a. 10. The method of claim 9, wherein the oleander plant is selected from at least one of flowers, leaves, branches, and roots of the oleander plant. 10. The method of claim 9, wherein the extract is treated at a concentration of 100-200 ppm in the cooling water.

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