KR20060136089A - Method for production of houttuynia decoction fermentation beverage - Google Patents
Method for production of houttuynia decoction fermentation beverage Download PDFInfo
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- KR20060136089A KR20060136089A KR1020050055616A KR20050055616A KR20060136089A KR 20060136089 A KR20060136089 A KR 20060136089A KR 1020050055616 A KR1020050055616 A KR 1020050055616A KR 20050055616 A KR20050055616 A KR 20050055616A KR 20060136089 A KR20060136089 A KR 20060136089A
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- lactic acid
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- 238000000855 fermentation Methods 0.000 title claims abstract description 53
- 230000004151 fermentation Effects 0.000 title claims abstract description 53
- 238000004519 manufacturing process Methods 0.000 title abstract description 21
- 235000013361 beverage Nutrition 0.000 title abstract description 5
- 235000013717 Houttuynia Nutrition 0.000 title 1
- 240000000691 Houttuynia cordata Species 0.000 title 1
- 235000013719 Houttuynia cordata Nutrition 0.000 title 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 63
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- 241001627205 Leuconostoc sp. Species 0.000 claims description 5
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- MKJXYGKVIBWPFZ-UHFFFAOYSA-L Calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 description 2
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
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- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 239000008213 purified water Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
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- 241001116389 Aloe Species 0.000 description 1
- 229920002261 Corn starch Polymers 0.000 description 1
- JVTAAEKCZFNVCJ-UWTATZPHSA-N D-lactic acid Chemical compound C[C@@H](O)C(O)=O JVTAAEKCZFNVCJ-UWTATZPHSA-N 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N D-sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- QBDCOUHKEVYWLO-UHFFFAOYSA-N Decanoyl acetaldehyde Chemical compound CCCCCCCCCC(=O)CC=O QBDCOUHKEVYWLO-UHFFFAOYSA-N 0.000 description 1
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- 229940045184 Malt extract Drugs 0.000 description 1
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- 230000003522 irritant Effects 0.000 description 1
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- 231100000021 irritant Toxicity 0.000 description 1
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- 229920001542 oligosaccharide Polymers 0.000 description 1
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- 235000019319 peptone Nutrition 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
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- 239000011780 sodium chloride Substances 0.000 description 1
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Images
Abstract
본 발명은 어성초 발효 음료의 생산방법에 관한 것으로, 본 발명의 방법에 의하여 제조된 어성초 발효 음료는 기존의 어성초 발효 음료에 비해 관능성과 젖산발효가 부여하는 기능성이 부과되어 품질이 매우 우수하다. The present invention relates to a method for producing a fermented beverage of Echochocho, the echochocho fermentation beverage produced by the method of the present invention is very excellent in quality due to the functionality imparted by the functionality and lactic acid fermentation compared to conventional echochocho fermentation.
어성초, 곶감, 루코노스톡, 음료 Eoseongcho, Dried persimmon, Lukonok stock, Drink
Description
도 1은 본 발명에서 분리한 루코노스톡 속(Leuconostoc sp.) 균주의 16s rDNA 염기서열이다. 1 is a genus Luconostok isolated ( Leuconostoc sp.) In the present invention 16s rDNA sequence of the strain.
도 2는 본 발명에서 분리한 루코노스톡 속(Leuconostoc sp.) 균주의 계통발생도이다.Figure 2 is a genus Luconostok isolated ( Leuconostoc sp.) In the present invention The phylogeny of the strain.
본 발명은 어성초 발효 음료의 생산방법에 관한 것이다. The present invention relates to a method for producing a fermented beverage of Eochocho.
어성초는 데카노일 아세트 알데하이드 등에서 유래하는 강력한 항균활성, 쿠르치드린 등에서 유래하는 이뇨작용, 페놀성 물질 등에서 유래하는 항산화활성 및 면역 증강작용 외에 항바이러스 작용, 지혈작용, 혈행개선작용, 조직재생 촉진작용 등의 우수한 약리활성을 나타내고 있어 기능성 식품 소재로 관심이 증가하고 있다. 그러나 어성초는 맛과 향이 강하여 생즙 또는 추출액을 그대로 섭취하기에는 관능적 적성이 부적합하다. Eochocho has strong antibacterial activity derived from decanoyl acetaldehyde, diuretic action derived from curchidrin, antioxidant activity and immune enhancement action derived from phenolic substances, etc., antiviral action, hemostatic action, blood circulation improvement, tissue regeneration activity It shows excellent pharmacological activity, and the interest is increasing as a functional food material. However, Eoseongcho has a strong taste and aroma, so it is not suitable for eating raw juice or extract as it is.
이에 어성초 특유의 자극적인 비린내와 독특한 맛을 개선하고자 다양한 발명들이 개시되어 있는데, 그 일 예로 대한민국특허청 공개특허공보 공개번호 10-2005-0043325호(2005년 5월 11일)에 어성초 발효 음료 조성물이 개시되어 있다. 상기 발명은 어성초 특유의 냄새가 제거되어 음용하기에 용이한 발효 음료를 제공하고 있으나, 발효균에 대한 구체적 언급이 없이 자연발효를 수행할 뿐이고, 실질적으로 어성초 특유의 냄새는 첨가되는 설탕이나 꿀에 의해서 상쇄될 뿐이다. Thus, various inventions have been disclosed to improve the irritant fishy and unique taste peculiar to Eochocho. For example, Eochocho fermented beverage composition is disclosed in Korean Patent Application Publication No. 10-2005-0043325 (May 11, 2005). Is disclosed. The present invention provides a fermented beverage that is easy to drink by removing the odor peculiar to Eochocho, but only performs natural fermentation without specific reference to the fermentation bacteria, and the odor unique to Echochocho is substantially added by sugar or honey to be added. It is only offset.
또한, 대한민국특허청 등록특허 10-0369638호(2003년 1월 29일)에 천연식물성 재료를 이용한 음료의 제조방법으로 어성초의 비린내를 없애고자 어성초엑기스에 알로에와 옥수수엿, 당, 벌꿀 등을 혼합하여 발효한 음료가 개시되어 있으나, 이 역시도 발효균에 대한 구체적 언급이 없고, 첨가되는 당 성분에 의해서 어성초의 비린 맛을 상쇄될 뿐이다.In addition, Korea Patent Office Registration No. 10-0369638 (January 29, 2003) in order to eliminate fishy fishy fish smell by using a natural vegetable material by mixing aloe, corn starch, sugar, honey, etc. Although fermented beverages are disclosed, there is no specific reference to the fermentation bacteria, but only the fishy taste of fish vinegar is offset by the added sugar component.
이상과 같이 기능성과 관능적성을 향상시킬 목적으로 어성초 즙에 당을 첨가하여 자연발효시키거나, 건조 어성초 열수 추출액에 올리고당 등을 포함하는 여러 가지 첨가물을 가하고 살균하여 제조한 어성초 음료의 경우 균일한 제품을 얻기가 어렵고, 알코올이 과도하게 생성되어 주세법상 주류로 분류되어 합법적으로 유통시키기 위해서는 주류면허를 받아야 하는 등의 문제가 발생한다. As described above, in the case of Eochocho beverages prepared by adding sugar to the Echochocho juice for the purpose of improving functionality and sensuality, or by adding various additives including oligosaccharides to the dried Echochocho hot water extract, the products are uniform. Difficulty in obtaining alcohol, alcohol is excessively generated and classified as liquor under the State Tax Law, so that the law requires a liquor license to distribute legally.
이에 본 발명의 목적은 적절히 조성된 어성초 추출액에 발효균주를 접종하여 발효시키고 이를 여과, 살균, 포장하는 일련의 가공과정을 통해 기능성과 기호성이 뛰어난 어성초 발효 음료의 생산방법을 제공하는데 있다.Accordingly, an object of the present invention is to provide a method for producing fermented beverages with excellent functionality and taste through a series of processes inoculating fermented strains by inoculating fermented strains with appropriately formulated fish extract and filtering, sterilizing and packaging them.
상기 목적을 달성하기 위하여 본 발명은, 어성초 추출액, 설탕을 혼합하고, 루코노스톡(Leuconostoc) G5 KACC 91156P 균주를 접종하여 배양액을 제조한 후 배양하는 단계; 발효 후, 고형물을 침전시키고 여과하는 단계; 및, 여과한 후, 살균을 실시하고 포장하는 단계를 포함하는 것을 특징으로 하는 어성초 발효음료의 생산방법을 제공한다.In order to achieve the above object, the present invention, Eochochocho extract, sugar, and inoculated with Leuconostoc (Luconostoc) G5 KACC 91156P strain to prepare a culture solution and culturing; After fermentation, solids are precipitated and filtered; And, after filtering, the sterilization and packaging process Eoseongcho fermented beverage, characterized in that it comprises a step of packaging.
이하, 본 발명에 대해 더욱 상세히 설명한다.Hereinafter, the present invention will be described in more detail.
본 발명은 루코노스톡 속(Leuconostoc sp.) KACC 91156P 균주를 제공한다. 상기 루코노스톡(Leuconostoc) G5 균주는 곶감으로부터 분리한 것으로 일반적으로 식품 발효에 응용할 수 있는 안전성이 확보된 미생물이다. 상기 균주는 통성혐기성, 그람 양성, 비운동성, 연쇄구균으로 루코노스톡 속 미생물의 배양학적 특성을 나타내며, 16S rDNA 염기서열에 기초한 계통분류학적 분석결과 루코노스톡 속 세균임을 확인하여(도 1 및 도 2 참조), 이를 루코노스톡 G5(Leuconostoc G5)로 명명하고, 2005년 3월 7일자로 농업생명공학원에 기탁하였다(기탁번호: KACC 91156P).The present invention is Leuconostoc sp. Provide the KACC 91156P strain. The Leuconostoc G5 strain is isolated from dried persimmon and is generally a microorganism secured to be applicable to food fermentation. The strain is aerobic anaerobic, gram-positive, non-motile, streptococcus, showing the culture characteristics of the microorganisms of the genus Lukonostok, and identified by the phylogenetic analysis based on 16S rDNA sequence to confirm that the bacteria of the genus Lukonostok (Fig. 1 and 2, which was named Leuconostoc G5 and was deposited with the Institute of Agricultural Biotechnology on March 7, 2005 (accession number: KACC 91156P).
한편, 상기 균주를 어성초 추출물에 접종하여 젖산발효를 유도하면 젖산발효 에 기인하여 어성초 특유의 냄새가 제거되고, 풍미가 개선된 어성초 추출물 발효액이 만들어진다. On the other hand, when the strain is inoculated into Eochocho extract to induce lactic acid fermentation, due to lactic acid fermentation, the smell of peculiar to Echochocho is removed, and the Echochocho fermentation broth with improved flavor is made.
본 발명에 있어 어성초 추출액은 열수추출에 의한 통상의 방법으로 추출된 것이라면 어떤 열수추출물을 어성초 추출원액으로 사용하여도 무방하나, 바람직스럽게 말린 어성초 100 g에 대하여 수돗물을 4 L의 비율로 가하여 1시간 끓인 후 여과한 후, 여액을 감압증발시켜 고형분량이 3 Brix 되도록 감압 농축한 어성초 추출액을 원액으로 사용하는 것이 좋다.In the present invention, the eochochocho extract can be used as a raw vinegar extract raw water if it is extracted by the usual method by hot water extraction, preferably 1 hour by adding tap water at a rate of 4L per 100g of dried eochochocho. After boiling and filtering, the filtrate was evaporated under reduced pressure, and the extract of Echochocho was concentrated under reduced pressure so that the solid content was 3 Brix.
한편, 본 발명은 젖산균의 본배양에 앞서 바람직스럽게 종배양을 할 수 있는데, 종배양액은 본 발명에서 분리한 루코노스톡(Leuconostoc) G5 KACC 91156P 균주를 MRS 배지 40 ml이 들어 있는 250 ml 삼각플라스크에 접종하고 25 ℃ 진탕 배양기에서 120 rpm으로 진탕하면서 2 일 간 배양하여 얻어지는 것이 좋다. On the other hand, the present invention can be a Preferably seed culture prior to the lactic acid bacteria of the incubation, the seed culture is Lu Pocono stock (Leuconostoc) isolated from the invention G5 KACC 91156P strains MRS medium 40 ml This example 250 ml Erlenmeyer flask It is good to obtain by inoculating in and incubated for 2 days while shaking at 120 rpm in a 25 ℃ shaking incubator.
한편, 본 발명의 어성초 추출액 젖산발효는 바람직스럽게 하기와 같이 수행하는 것이 좋다. 먼저, 어성초 추출액에 정제수를 가하여 고형분량이 2 Brix 되도록 조정하고, 설탕을 1 %(w/v) 첨가하여 발효배지를 조성한 후, 80 ℃에서 15 분 간 살균한다. 여기에 젖산균 종배양을 1 %(v/v) 접종하고 25 ℃에서 3 일 간 정치배양하여 젖산발효를 진행한다. 이때, 설탕을 첨가하는 경우는 그렇지 않은 경우와 비교하여 젖산균 증식 및 젖산 생성량이 훨씬 증가하는데, 바람직스럽게 첨가되는 설탕의 양은 3 %(w/v)인 것이 좋다.On the other hand, the lactic acid fermentation of Echochocho extract of the present invention is preferably carried out as follows. First, purified water is added to the extract of Eoseongcho so that the solid content is adjusted to 2 Brix, and 1% (w / v) of sugar is added to form a fermentation broth, followed by sterilization at 80 ° C. for 15 minutes. Inoculated with lactic acid
한편, 질소원을 첨가하는 경우 어성초 추출액의 젖산발효가 증진되는데, 질소원으로서 효모엑기스를 첨가한 경우에 젖산균 증식 및 젖산 생성량이 가장 높으 며, 첨가되는 효모엑기스의 양이 증가될수록 젖산균의 증식 및 젖산 생성량이 증대된다. On the other hand, lactic acid fermentation of the Echo extract is enhanced when nitrogen source is added, and when yeast extract is added as nitrogen source, lactic acid bacteria growth and lactic acid production are the highest, and as the amount of added yeast extract is increased, the lactic acid bacteria growth and lactic acid production amount are increased. Is increased.
한편, 발효 온도에 따른 어성초 추출액의 젖산발효 양상을 조사한 결과, 온도가 증가함에 따라 세포 증식속도는 35 ℃에서 가장 빠르나, 최종 젖산 생산량은 30℃에서 가장 높다. On the other hand, as a result of investigating the lactic acid fermentation pattern of Eochochocho extract according to fermentation temperature, the cell proliferation rate is the fastest at 35 ℃ as the temperature increases, but the final lactic acid production is the highest at 30 ℃.
한편, 본 발명은 건조 어성초 추출액, 설탕을 혼합하고, 루코노스톡(Leuconostoc) G5 KACC 91156P 균주를 접종하여 배양액을 제조한 후 배양하는 단계; 발효 후, 고형물을 침전시키고 여과하는 단계; 및, 여과한 후, 살균을 실시하고 포장하는 단계를 포함하는 어성초 발효음료의 생산방법을 제공한다.On the other hand, the present invention is dried dried vinegar extract, sugar, and inoculated with Leuconostoc (Luconostoc) G5 KACC 91156P strain to prepare a culture medium and then culturing; After fermentation, solids are precipitated and filtered; And, after filtering, sterilization and provides a method for producing a fermented beverage of Eochocho fermentation.
원료의 혼합으로서 발효음료 제조에 적합한 어성초 발효 배지성분의 배합은 바람직스럽게 2 brix로 조정된 건조 어성초 추출액 70L, 설탕 3Kg, 젖산발효균주 1 L 접종한 뒤 수돗물로 100 L로 맞춘 것이 좋다. 한편, 바람직스럽게 상기 발효음료에는 기호에 따라 첨가물로 음료의 제조에서 사용이 되는 정백당, 비타민 C, 벌꿀, 젖산칼슘을 사용할 수 있다. The mixing of the fermented broth fermentation medium ingredients suitable for fermented beverage production as a mixture of raw materials is preferably inoculated with 70L of dried fish extract, adjusted to 2 brix, 3Kg of sugar, 1L of lactic acid fermentation strain and then adjusted to 100L with tap water. On the other hand, preferably the fermented beverage can be used white sugar, vitamin C, honey, calcium lactate that is used in the manufacture of beverages as additives according to preference.
배합 후, 보일러의 온도 게이지와 사전에 예열을 통해 발효조의 온도 편차를 2℃ 내외로 조절하여 바람직스럽게 30 ℃에서 72 시간 배양한다. 이때 진탕배양을 위해 발효조 내 회전날개 속도를 바람직스럽게 2 rpm으로 조정하여 배양을 수행한다.After blending, the temperature deviation of the fermenter is adjusted to around 2 ° C. through preheating with a temperature gauge of the boiler in advance, and preferably incubated at 30 ° C. for 72 hours. At this time, the culture of the rotating wing in the fermenter is preferably adjusted to 2 rpm for shaking culture.
배양 후, 바람직스럽게 발효조와 공조 수송라인으로 연결된 500L 탱크에서 고형물을 30 분 간 침전시킨다. 그 후 바람직스럽게 규조토 여과기에서 적절한 압력으로 압출하여 여과를 실시한다. After incubation, the solids are preferably precipitated for 30 minutes in a 500L tank connected by fermenter and air conditioning transport line. The filtration is then carried out preferably by extruding at an appropriate pressure in a diatomaceous earth filter.
한편, 살균을 위해, 바람직스럽게 포장장치로 이송되는 과정중에 HTST(Hot Temperature Short Time)방식의 순간고온 살균을 3 초 간 실시한다. 그 후, 포장은 음료의 제조에 있어 당업계에서 사용되는 다양한 방법을 사용할 수 있으나, 바람직스럽게 토르트 파우치에 최종 생산된 제품을 충전하여 생산을 완료하는 것이 좋다.On the other hand, for sterilization, instantaneous high temperature sterilization of HTST (Hot Temperature Short Time) method is preferably performed for 3 seconds during the transfer to the packaging apparatus. Thereafter, the packaging may use a variety of methods used in the art in the manufacture of the beverage, but it is preferable to complete the production by filling the tort pouch with the final product.
이하, 본 발명의 구성 및 작용을 하기 실시예에 의하여 더욱 상세히 설명하지만 본 발명의 권리범위가 하기 실시예에만 한정되는 것은 아니다.Hereinafter, the configuration and operation of the present invention will be described in more detail with reference to the following examples, but the scope of the present invention is not limited to the following examples.
<실시예 1-젖산균 분리> Example 1-Lactic Acid Bacteria Separation
젖산균 선별Lactobacillus Screening
수집한 김치류, 채소류, 과일류를 멸균 생리식염수와 1:1의 무게비율로 혼합한 후 믹서로 균질화 하여 균원 시료로 사용하였다. 균원 시료를 적당히 희석하여 백금이로 젖산균 분리용 배지인 MRS한천배지에 도말하고 평판을 30 ℃의 항온기에서 72 시간 배양한 후 출현한 젖산균 집락을 같은 조성의 평판배지에서 순수분리 하였다. 순수하게 분리한 젖산균은 멸균한 설탕 1 %(w/v) 함유 어성초 추출물에 접종하고 25 ℃의 항온기에서 72 시간 정치배양한 후 맛과 향을 관능적으로 검토하여 우수한 균주를 선별하여 분리하였다. The collected kimchi, vegetables, and fruits were mixed with sterile saline in a weight ratio of 1: 1 and homogenized with a mixer to use as a fungal sample. The fungal samples were diluted appropriately and plated on MRS agar medium, which is a medium for separating lactic acid bacteria with platinum, and the plate was incubated for 72 hours at 30 ° C incubator. Purely isolated lactic acid bacteria were inoculated with sterilized
최종적으로 분리한 균주는 곶감으로부터 분리한 것으로 G5로 명명하여 이를 본 발명의 어성초 추출물 발효균주로 선별하였다. 선별한 균주는 MRS한천배지에 계 대배양하며 보관하였다.Finally, the isolated strain was isolated from dried persimmon and named G5 and selected as the fermented strain of Eochochocho extract of the present invention. Selected strains were stored subcultured in MRS agar medium.
분리 균주의 미생물학적 특성Microbiological Characteristics of Isolated Strains
상기에서 분리한 G5균주를 MRS한천배지에 접종하고 30 ℃에서 2 일 간 배양한 결과 직경 1 ~ 1.5 mm 크기의 유백색 불투명한 집락을 형성하였으며, 현미경 관찰시 전형적인 연쇄구균으로 관찰되었으며, 그램 염색약에 대한 반응은 그램 양성으로 관찰되었다. The isolated G5 strain was inoculated in MRS agar medium and incubated at 30 ° C. for 2 days to form a milky white opaque colony with a diameter of 1 to 1.5 mm, and was observed as a typical streptococcus microscopically. Response was observed to be gram positive.
G5 균주가 어느 속에 포함되는지 명확히 하기 위해 16S rDNA 염기서열을 분석하였다(도 1 참조). 이 염기서열을 미국 진뱅크(GenBank) 데이터베이스로부터 얻은 세균학적으로 연관되어 있을 것으로 추정되는 연관 종의 16S rDNA 염기 서열과 크러스탈 더블류 (CLUSTAL W) 소프트웨어를 사용하여 계통분류학적 분석을 시도하였는데, 그 결과 도 2에서 보는 바와 같이 루코노스톡 속(Leuconostoc genus)에 속하는 균주 임을 알 수 있었다. 따라서, G5 균주는 루코노스톡 (Leuconostoc sp.)에 속하는 젖산균으로 루코노스톡(Leuconostoc) G5로 명명하여 이를 농업생명공학원에 2005년 3월 7일자로 기탁하였다(수탁번호: KCTC 91156P).16S rDNA sequences were analyzed to clarify which genus G5 strains were included (see FIG. 1). This sequence was subjected to phylogenetic analysis using the 16S rDNA base sequence of the related species suspected of being bacteriologically derived from the US GenBank database and the CRUSTAL W software. As a result, as shown in Figure 2 it can be seen that the strain belonging to the genus ( Leuconostoc genus). Therefore, the G5 strain was named as Leuconostoc G5 as a lactic acid bacterium belonging to Leuconostoc sp. And it was deposited on March 7, 2005 at the Institute of Agricultural Biotechnology (Accession Number: KCTC 91156P).
한편, 상기의 미생물학적 특성을 밝히는 실험은 카푸치노와 셔먼의 방법(Cappuccino, James G. and Sherman, Natalie, MICROBIOLOGY, A LABORATORY MANUAL, 4th ed. The Benjamin/Cumming Publishing Inc., Menlo Park, California, 1996)과 윤 등의 방법(Yoon et al., Int. J. Sys. Bacteriol. 48, 833-837, 1998)에 준하여 실시하였다.On the other hand, experiments that reveal the microbiological characteristics of Cappuccino and Sherman (Cappuccino, James G. and Sherman, Natalie, MICROBIOLOGY, A LABORATORY MANUAL, 4th ed.The Benjamin / Cumming Publishing Inc., Menlo Park, California, 1996 ) And Yoon et al . (Yoon et al ., Int. J. Sys. Bacteriol. 48, 833-837, 1998).
<실시예 2-어성초 추출액 젖산발효>Example 2 Lactic Acid Fermentation
어성초 추출액 조제Preparation of Eoseongcho Extract
말린 어성초 100 g에 대하여 수돗물 4 L를 가하여 1시간 끓인 후 여과하였다. 그 후 여액을 감압증발시켜 고형분량이 3 Brix 되도록 감압 농축하여 어성초 추출액 원액을 조제하였다. 4 L of tap water was added to 100 g of dried fish vinegar, and the mixture was boiled for 1 hour and filtered. Thereafter, the filtrate was evaporated under reduced pressure, concentrated under reduced pressure so as to have a solid content of 3 Brix, to prepare a stock solution of the extract.
젖산균 종배양Lactobacillus Species Culture
실시예 1에서 분리한 루코노스톡(Leuconostoc) G5 KACC 91156P 균주를 MRS 배지 40 ml이 들어 있는 250 ml 삼각플라스크에 접종하고 25 ℃ 진탕 배양기에서 120 rpm으로 진탕하면서 2 일 간 배양하여 어성초 추출액의 젖산발효를 위한 종배양을 조성하였다. Example 1 A base Pocono separated from the stock (Leuconostoc) G5 KACC 91156P strains MRS medium 40 ml is 2 days and inoculated into a 250 ml Erlenmeyer flask containing and shaken at 120 rpm at 25 ℃ shaking incubator culturing of Houttuynia cordata extract, lactic acid Species cultures were made for fermentation.
당 첨가에 의한 어성초 추출액의 젖산발효Lactic Acid Fermentation of Estuary Extracts by Sugar Addition
상기 어성초 추출액에 정제수를 가하여 고형분량이 2 Brix 되도록 조정하고 설탕을 1 %(w/v) 첨가하여 발효배지를 조성하고 80 ℃에서 15 분 간 살균하였다. 여기에 상기 젖산균 종배양을 1 %(v/v) 접종하고 25 ℃에서 3 일 간 정치배양으로 젖산발효를 진행하였다. Purified water was added to the extract of Eoseongcho so that the solid content was adjusted to 2 Brix, and 1% (w / v) of sugar was added to form a fermentation broth and sterilized at 80 ° C. for 15 minutes. The lactic acid bacteria species culture was inoculated with 1% (v / v), and the lactic acid fermentation was performed in a stationary culture at 25 ° C. for 3 days.
설탕을 첨가하지 않은 경우와 비교하여 젖산균 증식 및 젖산 생성량이 훨씬 증가하여 젖산발효가 촉진됨을 알 수 있었다(표 1). 젖산 생성량은 발효액을 0.1N-NaOH로 적정하여 소비된 NaOH 량을 젖산으로 환산하였으며 생균수는 적당히 희석한 시료를 MRS 한천배지에 도말하여 형성된 집락수를 계수하여 산출하였다.Compared to the case where no sugar was added, it was found that lactic acid bacteria growth and lactic acid production were much increased, thereby promoting lactic acid fermentation (Table 1). The amount of lactic acid produced was calculated by counting the colonies formed by smearing the fermentation broth with 0.1N-NaOH and converting the amount of NaOH consumed into lactic acid, and the viable cell number was smeared onto MRS agar medium.
질소원 첨가에 의한 어성초 추출액의 젖산발효Lactic Acid Fermentation of Estuary Extracts by Addition of Nitrogen Sources
상기 1 %(w/v) 설탕이 첨가된 어성초 추출액에 몇 가지 질소원을 0.1 %(w/v)되도록 첨가하여 상기한 방법을 젖산발효를 진행시킨 결과 표 2와 같았다. 표 2에서 보는 바와 같이 질소원으로서 효모엑기스를 첨가한 경우에 젖산균 증식 및 젖산 생성량이 가장 높았다. As a result of proceeding the lactic acid fermentation by adding several nitrogen sources to 0.1% (w / v) in the Echo extract extracted with the 1% (w / v) sugar was as shown in Table 2. As shown in Table 2, when yeast extract was added as a nitrogen source, lactic acid bacteria growth and lactic acid production were the highest.
설탕 농도를 달리한 어성초 추출액의 젖산 발효Lactic Acid Fermentation of Estuary Extracts with Different Sugar Concentrations
질소원으로서 효모엑기스 0.1 %(w/v) 첨가된 어성초 추출액에 설탕농도를 다르게 첨가하여 상기한 방법으로 젖산발효를 진행시킨 결과 표 3과 같았다. 표 3에서 보는 바와 같이 설탕을 3 %(w/v) 첨가하는 경우에 젖산균 증식 및 젖산 생성량이 가장 높았다. As a result of the lactic acid fermentation by adding the sugar concentration differently to the yeast extract added with 0.1% (w / v) yeast extract as a nitrogen source, it was as shown in Table 3. As shown in Table 3, the addition of 3% (w / v) of sugar showed the highest lactic acid bacteria growth and lactic acid production.
효모엑기스 농도를 달리한 어성초 추출액의 젖산발효Lactic Acid Fermentation of Estuary Extracts with Different Yeast Extract Concentrations
설탕 3% (w/v) 첨가된 어성초 추출액에 질소원으로서 효모엑기스를 다르게 첨가하여 상기한 방법으로 젖산발효를 진행시킨 결과 표 4와 같았다. 표 4에서 보는 바와 같이 효모엑기스 첨가량 증가에 따라 젖산균 증식 및 젖산 생성량이 증가되는 경향을 나타냈다.The yeast extract as a nitrogen source was added to the extract of Eocho vinegar added with 3% (w / v) sugar and the lactic acid fermentation was carried out by the above-described method. As shown in Table 4, as the amount of yeast extract added increased, the growth of lactic acid bacteria and the amount of lactic acid increased.
발효 온도에 따른 어성초 추출액의 젖산발효Lactic Acid Fermentation of Echinacea Extracts with Different Fermentation Temperatures
설탕 3 %(w/v) 및 효모엑기스 0.5 %(w/v)가 함유된 어성초 추출액의 젖산발효를 온도를 달리하여 25 ℃, 30 ℃, 및 35 ℃에서 발효를 진행시킨 결과 각각 표 5, 표 6, 및 표 7과 같았다. 표에서 보는바와 같이 온도가 증가함에 따라 세포 증식속도는 35 ℃에서 가장 빨랐으나, 최종 젖산 생산량은 30℃에서 가장 높았다. The fermentation of lactic acid fermentation of Echo extract containing 3% (w / v) sugar and 0.5% (w / v) yeast extract was carried out at 25 ° C, 30 ° C, and 35 ° C by varying the temperature, respectively. It was as Table 6 and Table 7. As shown in the table, the cell proliferation rate was the fastest at 35 ℃ as the temperature increased, but the final lactic acid production was the highest at 30 ℃.
<실시예-3> 어성초 발효 음료의 제조Example 3 Preparation of Echoseong Fermented Beverage
1. 재료의 준비와 전처리1. Preparation and Pretreatment of Materials
가. 어성초end. Eoseongcho
- 수확한 어성초를 수돗물로 세척하여 적절히 탈수하여 강제열풍건조장치를 통해 건조시켜 사용하였다. -The harvested fish was washed with tap water and dehydrated properly and dried by forced hot air dryer.
나. 젖산발효균주I. Lactic acid fermented strain
- 본 발명의 루코노스톡 속(Leuconostoc sp.) KACC 91156P 균주 -Leuconostoc sp. Of the present invention KACC 91156P strain
다. 첨가물All. additive
- 정백당, 비타민 C, 벌꿀, 젖산칼슘을 이용하였다. -White sugar, vitamin C, honey, calcium lactate was used.
2. 원료의 혼합2. Mix of raw materials
가. 어성초 발효액의 배합 : 2 brix로 조정된 건조 어성초 추출액 70 L, 설탕 3 Kg, 젖산발효균주 1 L 접종한 뒤 수돗물로 100 L로 맞췄다.end. Formulation of fermented broth of Eochocho vinegar: 70 L of dried Echochocho extract adjusted to 2 brix, 3 Kg of sugar, 1 L of lactic acid fermentation strain and inoculated to 100 L with tap water.
3. 발효3. Fermentation
- 보일러의 온도 게이지와 사전에 예열을 통해 발효조의 온도 편차를 2 ℃ 내외로 조절하여 30 ℃에서 72 시간 배양했다. The temperature range of the fermenter was adjusted to around 2 ° C by incubating in advance with the temperature gauge of the boiler and pre-heated for 72 hours at 30 ° C.
- 진탕배양을 위해 발효조 내 회전날개 속도를 2 rpm으로 조정하였다. The rotor blade speed in the fermenter was adjusted to 2 rpm for shaking culture.
4. 여과살균4. Filter sterilization
- 발효조와 공조 수송라인으로 연결된 500 L 탱크에서 고형물을 30 분 간 침전시켰다. The solids were allowed to settle for 30 minutes in a 500 L tank connected to the fermenter and the air conditioning transport line.
- 규조토 여과기에서 적절한 압력으로 압출하는 방법으로 여과를 실시하였다. Filtration was carried out by extrusion at an appropriate pressure in a diatomaceous earth filter.
- 포장장치로 이송되는 과정중에 HTST(Hot Temperature Short Time)방식의 순간고온 살균을 3 초 간 실시하였다. -During the process of transfer to the packaging device, instantaneous high temperature sterilization of HTST (Hot Temperature Short Time) was performed for 3 seconds.
- 레토르트 파우치에 최종 생산된 제품을 충전하여 생산을 완료하였다. -The retort pouch was filled with the final product to complete the production.
본 발명의 방법에 의하여 제조된 어성초 발효 음료는 기존의 어성초 추출액에 비해 관능성과 젖산발효가 부여하는 기능성이 부과되어 품질이 매우 우수하다.The fermented beverage of Echochocho prepared by the method of the present invention has excellent quality due to the functionality imparted by the functionality and lactic acid fermentation compared to conventional Echochocho extract.
Claims (5)
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