KR20060057863A - Testis-specific nucleotide sequences - Google Patents

Abstract

The present invention relates to a testis-specific nucleotide sequence and a method for identifying testis cells using the same. Since the nucleotide sequence of the present invention is specifically expressed in the testis, it can be very useful for identification of testicular cells, sex discrimination, segregation of sperm, treatment of infertility or infertility, and gene diagnosis, as well as control of spermatogenesis. Provides basic information for studying a series of mechanisms.

Testis, Nucleotide, Testis-specific, Animal, Mammal, Bird, Chicken

Description

Testis-Specific Nucleotide Sequences             

1A and 1B are graphs showing the number of tentative consensus sequences (TCs) showing positive response in RT-PCR using total RNA obtained from chicken tissue. The total number of TCs amplified from each tissue was compared with the number of tissue-specific TCs. 110 testis-specific TCs were found, but no other tissue-specific TCs (FIG. 1A). PCR products of 19 TCs showed different patterns from estimated size in tissues other than testis (FIG. 1B).

2 is a graph showing the number of positive TCs sorted by the number of assembled ESTs forming each TC.

3A-3C are graphs showing the percentage of TCs sorted according to the Gene Ontology (GO) index. Amplified cDNAs according to the GO category were classified into biological processes (FIG. 3A), molecular functions (FIG. 3B) and cellular components (FIG. 3C).

4A and 4B are graphs comparing the distribution of testicular-specific TCs and total TCs in chickens against the molecular functional categories of the gene ontology index, FIG. 4A for chickens and FIG. 4B for humans.

5A-5F are photographs of RT-PCR results showing testicular-specificity of the nucleotides of the present invention.

The present invention relates to testis-specific nucleotide sequences and methods of identifying testis cells.

Testis is the organ that produces sperm, and during spermatogenesis, the regulation of transcription in germ cells is very finely regulated [1]. Sperm develop with somatic testicular cells, which have high specificity, such as Sertoli and Leydig cells. While germ cells differentiate into spermatozoa, complex paracrine conversations with sertoli cells occur [2]. Endocrine activity, such as testosterone secretion by stromal cells, promotes the differentiation of germ cells [3]. Thus, it is believed that the testes will have specific transcriptional complexes that incorporate spermatogenesis differentiation programs.

In birds, the female is heterozygous (ZW), but the genes of the W chromosome do not affect genital development, similar to the SRY gene of the mammalian Y chromosome. No sex-chromosome-specific SOX genes similar to the mammalian sex-determining gene SRY were found in birds [4]. However, SRY-like HMG-box gene 9 ( SOX9 ) affects genital development by initiating transcription of anti-Mllerian hormone (AMH) during the early stages of chicken genital differentiation [5]. The avian DMRT1 gene is located on the Z chromosome [6] and is more strongly expressed in males than in female embryonic genitalia [7-9].

Therefore, it is believed that many genes in birds will affect male germ cell development and some of these genes will be expressed in testis-specific patterns. Chickens are the most important model organism for the study of germline development because chicken embryo development occurs in in ovo .

Throughout this specification many patent documents and articles are referenced and their citations are indicated. The disclosures of cited patent documents and articles are incorporated herein by reference in their entirety, so that the level of the technical field to which the present invention belongs and the contents of the present invention are more clearly explained.

As a preparatory work to identify a series of mechanisms of spermatogenesis, the present inventors have made diligent research efforts to identify nucleotide sequences that are specifically expressed in testes. The present invention was completed by confirming through the bioinformatics method and in vitro experiments.

Accordingly, it is an object of the present invention to provide a testis-specific nucleotide sequence.

Another object of the present invention is to provide an avian testis-specific nucleotide sequence.

Another object of the present invention is to provide a method for identifying testis cells.

Other objects and advantages of the present invention will become apparent from the following detailed description, claims and drawings.

According to an aspect of the present invention, the present invention comprises a nucleotide sequence selected from the group consisting of SEQ ID NO: 1 to 110, a sequence complementary to the nucleotide sequence or a fragment of the nucleotide, characterized in that Provide a testis-specific nucleotide sequence.

We have studied to identify nucleotide sequences that are specifically expressed in testes as a preparatory work to elucidate a series of mechanisms of spermatogenesis. We have studied a variety of avian organs (eg, testes, brain, spleen, liver, muscle and ovaries) and as a result have found testicular-specifically expressed nucleotide sequences.

Testicular-specific nucleotide sequences of the present invention may be of animal, preferably mammal or avian, more preferably avian, even more preferably chicken, quail, turkey, duck, goose, pheasant and pigeon, most preferably chicken A nucleotide sequence that is specifically expressed in testes.

The nucleotide sequence of the present invention includes not only a nucleotide sequence selected from the group consisting of SEQ ID NOs: 1 to 110, but also a complementary sequence to the sequence. Complementary sequences of the present invention include not only perfectly complementary sequences, but also substantially complementary sequences. The term “substantially complementary sequence” means a sequence that can hybridize to a nucleotide sequence selected from the group consisting of SEQ ID NOs: 1 to 110 under stringent conditions known in the art. The term "stringent conditions" are defined by Joseph Sambrook, et al., Molecular Cloning, A Laboratory Manual , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (2001) and Haymes, BD, et al., Nucleic Acid Hybridization, A Practical Approach , IRL Press, Washington, DC (1985), stringent conditions can be determined by controlling temperature, ionic strength (buffer concentration), the presence of compounds such as organic solvents, and the like, depending upon the sequence being hybridized. have. For example, stringent conditions may be washed with (i) 50 ° C. temperature and 0.015 M sodium chloride / 0.0015 M sodium citrate / 0.1% sodium dodecyl sulfate, or (ii) hybridization buffer (50% formamide, 2 × SSC and Hybridization to 55 ° C. in 10% dextran sulfate), followed by washing at 55 ° C. with EDTA-containing 0.1 × SSC.

On the other hand, the nucleotide sequence of the present invention includes a fragment of the nucleotide sequence selected from the group consisting of SEQ ID NO: 1 to 110.

As described in the Examples below, the nucleotide sequences of the present invention contribute to molecular functions, biological processes and cellular components. Of the sequences classified into molecular functional groups, some are involved in binding and others are involved in catalytic activity. Of the sequences that contribute to biological processes, some encode proteins that participate in physiological processes, others encode proteins that participate in intracellular processes, and others encode proteins involved in development. Almost all of the sequences classified as cellular components are distributed within cells.

Many genes in animals are believed to affect male germ cell development, and some of these genes are believed to be expressed in testis-specific patterns. Testes-specific multiple nucleotide sequences identified in the present invention support this consensus in the art.

The nucleotide sequence of the present invention can be used for the following purposes because of testicular specificity: (iii) identification of testicular cells; (Ii) discrimination of sex; (Iii) segregation of sperm; (Iii) treating or inducing infertility; And (iii) genetic diagnosis (in particular, genetic diagnosis for infertility). For example, Northern Blotting (Total B. Kaufma et al., Molecular and Cellular Methods in Biology and Medicine , 102-108, CRC press) using total RNA from cells obtained from early embryos (embryo) The cDNA can be obtained from the total RNA and then subjected to hybridization to distinguish sex. If the nucleotide sequence of the present invention is used for infertility treatment or infertility, the testicular-specific nucleotide sequence of the present invention can be treated by injecting the sequence of the present invention into testicular cells of an infertile animal subject to infertility treatment. There is a possibility. In contrast, infertility can be induced by manipulating the nucleotide sequence of the invention in testicular cells of an animal subject to inhibit expression. In addition, the nucleotide sequence of the present invention can be used for gene diagnosis, in particular for gene diagnosis based on single nucleotide polymorphism.

According to another aspect of the present invention, the present invention provides a method for identifying testis cells, comprising the steps of: (a) DNA obtained from the cells or fragments thereof according to the avian testicular-specific nucleotide sequence of the present invention described above; Performing a hybridization reaction; And (b) confirming hybridization in step (a).

"Testicular cells" identified by the methods of the present invention include garden stem cells, garden cells including all germ cells derived from garden stem cells, sperm (sperm or spermatozoa), sertoli cells, stromal cells and others. Means a cell group in the testis tissue including muscle cells related to the connective tissue, preferably the stem cells, the garden cells and sperm, more preferably the sperm, most preferably sexually mature) means sperm.

In the method of the present invention, the method for obtaining DNA from the cells to be tested is preferably cDNA obtained through reverse transcription from total RNA isolated from the cells. Total RNA isolation can be performed according to conventional methods known in the art (see Sambrook, J. et al., Molecular Cloning. A Laboratory Manual , 3rd ed. Cold Spring Harbor Press (2001); Tesniere, C et al., Plant Mol. Biol. Rep. , 9: 242 (1991); Ausubel, FM et al., Current Protocols in Molecular Biology , John Willey & Sons (1987); and Chomczynski, P. et al., Anal. Biochem. 162: 156 (1987)). For example, Trizol can be used to easily isolate total RNA in cells. Obtaining cDNA from total RNA can be facilitated by reverse transcriptase (PCR) method.

The DNA thus obtained (eg cDNA) is preferably labeled. Such labels are made by substances that can be detected by spectroscopic, photochemical, biochemical, bioelectronic, immunochemical, electrical, optical, or chemical measurements. For example, the labeling material includes radioisotopes such as P ³² and S ³⁵ , chemiluminescent compounds, labeled binding proteins, heavy metal atoms, spectroscopic markers such as fluorescent markers and dies, and magnetic labels, It is not limited to this. Such dies include, but are not limited to, for example, quinoline dies, triarylmethane dies, phthaleins, azo dies and cyanine dies. Non-limiting examples of such fluorescent markers include fluorescein, phycoerythrin, rhodamine, lissamine, and Cy3 and Cy5 (Pharmacia). Labeling by labeling materials can be performed in a variety of ways conventionally practiced in the art, such as nick translation methods, random priming methods (Multiprime DNA labeling systems booklet, "Amersham" (1989)), and chination methods (Maxam). & Gilbert, Methods in Enzymology , 65: 499 (1986)).

In the method of the present invention, the hybridization reaction of DNA obtained from the cell with the testicular-specific nucleotide sequence is performed by Southern, E. J. Mol. Biol. Based on the hybridization process disclosed in 98: 503 (1975), it is carried out in accordance with optimized hybridization conditions by varying various conditions (salt concentration, temperature, reaction time, probe concentration) (Joseph Sambrook, et al. , Molecular Cloning, A Laboratory Manual , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (2001)). For example, it may be carried out by hybridizing to 55 ° C. in hybridization buffer (including 50% formamide, 2 × SSC and 10% dextran sulfate), followed by washing at 55 ° C. with EDTA-containing 0.1 × SSC.

In the method of the present invention, the step of confirming hybridization may be variously performed according to various methods commonly used in the art, in particular, the type of the labeling material used for the label. For example, when a fluorescent material is used as a labeling material, it can be confirmed by a fluorescence microscope, preferably a confocal fluorescence microscope, and the intensity of the signal detected through such a device increases in proportion to the degree of hybridization.

The method of the present invention is applied to an animal, preferably a mammal or a bird, more preferably a bird, even more preferably a chicken, quail, turkey, duck, goose, pheasant and pigeon, most preferably chicken Make sure that you correctly identify

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it is to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. Will be self-evident.

Example

Experimental Materials and Methods

group

The white leghorn chickens used in this study were maintained at Seoul National University Laboratory. Six tissues (sperm, brain, spleen, liver, muscle and ovary) were obtained from mature chickens (25 weeks old). Except for testes and ovaries, the remaining tissues were obtained from male and female chickens.

mRNA extraction and RT-PCR

Samples of chicken tissue were homogenized and then total RNA was obtained with Trizol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol. For each tissue, cDNA was synthesized from 0.5 μg total RNA in 20 μl reaction solution using Superscript III first chain synthesis system (Invitrogen).

Touchdown PCR was performed to prevent false band formation. Each PCR was performed using 2 μl cDNA per 20 μl reaction solution and 0.5 U of Taq polymerase (Biotools, Madrid, Spain). The initial denaturation step was conducted at 94 ° C. for 2 minutes, followed by 5 rounds of touchdown cycle. Primer denaturation was performed at 62 ° C. in the first cycle and decreased 0.5 ° C. each time in the subsequent cycle. In the following 25 cycles, amplification was carried out using a temperature cycle of 30 seconds at 94 ° C, 30 seconds at 60 ° C and 30 seconds at 72 ° C. The temperature cycle program was terminated at 72 ° C. for 7 minutes to allow complete extension reactions. On the other hand, the primer sequences used are shown in Table 1 below.

PCR amplification of all cDNA samples was carried out using primers for amplifying the house-keeping gene glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) as a control for RNA extraction and cDNA synthesis.

ESTs search and primer design

To identify testis-specific tentative consensus (TCs), the Gallus gallus Gene Index (GgGI) of the Institute for Genomic Research (TIGR) was searched and the searched sequences were assembled into TCs. Primer pairs for RT-PCR for each of the TCs were designed using the Primer3 program (http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi).

Gene Ontology Annotation and Bioinformatics Analysis

Testis-specific genes were classified using terms from the Gene Ontology (GO) database. Since many of these chicken sequences are not yet annotated, gene annotation was performed as follows: 1) extraction of information in a term already annotated, and 2) experimental annotation based on protein family database search (Pfam). Running. The latter method is based on indirect experimental evidence. Termination was based on protein domains using the GO term. Protein sequences were obtained from 110 testes-specific genes using the ESTScan program [10]. Protein domains for these protein sequences using HMMER 2.3.2 (http://hmmer.wustl.edu/) and the Pfam-A family matrix (http://www.sanger.ac.uk / Software / Pfam /) Search was conducted. E-value was set to 1 as a search variable, and trusted cutoff and noise cutoff were also used. Termination was done based on the Pfam2Go translation table, which shows the protein domain with the GO term.

The testicular-specific gene sequence of the present invention was aligned against a draft chicken genome sequence in the Genome Browser at the University of Santa Cruz, California. The distribution of testes-specific gene numbers on each chromosome was compared with an estimated number of genes on each chromosome. Chi-square tests were performed to determine the differences in these distributions. Exon numbers in each gene were determined from testes—especially the alignment of transcript and genome sequences.

Experiment result

Tissue specificity

A total of 292 experimental consensus sequences (TCs) were found in the library at GgGI of TIGR. Of these, 110 species (38%) of the sequence were expressed testicularly, which was confirmed by RT-PCR. The most TCs among candidate TCs were expressed in the testis, followed by the brain, spleen, muscle, liver and ovary. However, organ-specific TCs appear only in the testis (FIG. 1A). Nineteen of the first 292 candidate TCs were expressed as alternative splicing transcripts (FIG. 1B). PCR products amplified from testes had predicted size, but bands generated from RNA extracted from other tissues showed unpredicted size, indicating the possibility of tissue-specific alternative splicing. This difference between tissues is due to the complex regulatory mechanisms regulating tissue-specific gene expression. Some genes are known to exhibit different splicing patterns in different tissues [11, 12], which lead to the production of different proteins or affect the regulation of individual gene products [13].

A large number of candidate TCs and testicular-specific TCs were assembled from two ESTs (51% of total candidate TCs and 48% of testicular-specific TCs; FIG. 2), while 48 of the testicular-specific TC sequences were 3 More than ESTs were included. Three of the nine singleton sequences were testicular-specific. There was no significant correlation between the number of ESTs assembled into TCs and the number of testicular-specific TCs.

On the other hand, the sequence of the primers used in the RT-PCR and the size of the amplification products are summarized in the following Table 1a-1g, RT-PCR results photograph showing the testicular-specificity of the nucleotides of the present invention are shown in Figures 5a-5f have.

SEQ ID NO: Primer sequence Amplification Product Size (bp) First sequence CCAGCTCCTTCTCTCCACAG 508 CTCCCTTATAGGCACCAACG 2nd sequence GGAAGATCAGCCTTCACAGC 604 AATTTGGCAAACCAGACAGC Third sequence CATGGCTTTCAGGTTGGATT 764 ATGAGGAACTCCACCACGTC 4th sequence ATGCAGGACCCAAATGAAGA 773 TTGAGCCTGAACGGATTACC 5th sequence CAGGTTGCATCCTCCAGTTT 613 CCAATGACTCCTCGTCCACT 6th sequence GAAGGCTGACCACCTAGTGC 758 GGTCTGTGTCTGCTGTCCAA Seventh Sequence GGACAGCACACAGAGAGCAG 692 CCAGAAGAGCTCATTGCACA 8th sequence GCTGTATTTGCAGCCTGTGA 850 CACTGAATCAGCGAAGGTGA Ninth sequence CCTCTGGAGCAGGGATAACA 826 AAAGCAGGAGCAGAACCAGA 10th sequence ATACTCCGCCAGCTAAGCAA 747 AGGGATGGTGACTGAACCAG 11th sequence CTGCGATGCTACGACATCCT 278 TCGGAAGTTCTGTTCGGTCT Article 12 TGTCAGTCTGCCATTTGTCC 779 CAGCTGCGATCTTTCATGTT The thirteenth sequence GCTGTGCCTACACTGTGTCC 625 GCTAGGGCATAGCAGAGCAG Article 14 GGCCCATGGCTTATCACTAC 629 CATCAGAGGGCCACCACTAT Sequence 15 TTGTCGAGCCTGCTGTAATG 766 TCATCTGTAGCGGCACAAAC

SEQ ID NO: Primer sequence Amplification Product Size (bp) 16th sequence CTGGCAGGTGACAGAGAACA 703 ATTTGGGCCACACTCAGAAC SEQ ID NO: 17 AGTGCCTCGTCGGAATACAG 407 CCAATGTCCCAATGAAGGAC Article 18 CAGTCATCTGGGCTCTCCTC 739 GGGCCGGTAGAAATAGATGA Article 19 GAATGCAAAGAGGCTGAAGG 993 GGAACATCTGTGGGCTGAAT Article 20 AAGGAGTAACCACGGTGTGC 794 CCTCCAGCAGTATTCCCTGA Article 21 GCAAAGGACTTCCTGAAGCA 558 CACGAGTGCCTCATCTTTGA Article 22 CAAACAGGCTCAAGGAAGGA 824 ACCATTTGTTGGTGCTCCTC SEQ ID NO: 23 CTGGCGGTGTTGACTGTTTA 762 AATTGTCTTCTCCGGTGGTG Article 24 ACCCAAAGCAAACTGGATCA 702 AAGGATGATCGATGCGTTTC Article 25 AGCTGGGAGCTCAGGACAG 709 AGAGGTCTGACTCGGCCATA Article 26 GCTGTTCCCTGTGTCTGTCA 784 CGCTACTCAAGGGACAGGAG Article 27 TGCACCTGACTGGTACTTGC 508 TGGCTTCAATTCATTGCTTG Article 28 GTGCTGCCTATCCCTCTCTG 730 GGCCAGCAAGAACTTCAGTC SEQ ID NO: 29 ATGCTGTTGTCGAGTTGCTG 815 ACCATGTCTGGTTCCAGGAG Sequence 30 AGAAGGTGTCCCTCATTTGC 717 CTGACAAGGCCTGGATGTTT SEQ ID NO: 31 TCCACTCAGGGAAGGAGAGA 721 TTCATCGATCTGGTTGGTCA SEQ ID NO: 32 TCAAGGTCCTCGAGTGGATT 603 TGTGCAAGGTGGTGTCTTGT

SEQ ID NO: Primer sequence Amplification Product Size (bp) SEQ ID NO: 33 AGATTTGGGCCTGAAGTCCT 633 GTGCTTCTTGGGATCTCAGC SEQ ID NO: 33 GAAGGCAAACCACATGATGA 795 CCCGGTAGCTCCTCTTACCT SEQ ID NO: 33 CCCTCCAAACTGCTGTTCAT 728 ATCTGCAAGGCAGAGAGGAA SEQ ID NO: 33 CAAATCGTCTGCGAAACTCA 811 TTGCCAGTCCCTCTACCATC SEQ ID NO: 33 CAACTGGAAGGCATGTCTCA 539 CCGCCGGAGTAGAGTGTTTA SEQ ID NO: 33 AATGGGACACATACGGAAGC 404 ACACAGCCTCACCAACAGC SEQ ID NO: 33 TGCTCCTTTCTGGGTCTCAT 649 AGTCTGGTCTTGCAGCCATT SEQ ID NO: 40 GGAAGGGATTCCAACAGAGG 552 AGCAGGAAGCAAAGAACAGG SEQ.41. TGGAGCATGCTGAGGTGAT 722 AGGATCGTTGTTGAGCCATC SEQ ID NO: 42 CCTGCTGAGATCAAGAACCA 453 TGTTCTTTGCAGGTGGTGTT SEQ ID NO: 43 ATCCTGTGCACAATGTCAGG 406 GGAAGCTCTCGAACATCAGC Sequence 44 GCACAGGATGCCCTAAGAAA 718 CTTGCGCATCCTCACTAACA Sequence 45 GCGGGCGTCTGTGATTATAC 754 TTTCAGGCTGACATGCACA SEQ ID NO: 46 TAATTCTGACCGGTGCCAAC 439 CACAGAGCGCACAAACTTCT SEQ ID NO: 47 TATTCCAGAAGACGCGATCC 501 GTATCCCAGCAGGTGGTGTT SEQ ID NO: 48 TTCATTCAGTGGCATCAAGG 752 CTCCACTGCAGCTTTCCTTT SEQ ID NO: 49 CTTGCTCACGGTACCCAAA 608 TCTGTGGTGCCGTAGTTCTG

SEQ ID NO: Primer sequence Amplification Product Size (bp) Sequence 50 GGGATGGGATGTTATGTTCG 624 TTCAGCAGGTTTCTGGCTCT Sequence 50 TGGCTAAACTACAGGGAGCA 787 TAGCCCGGTTTCACTCTTGT Sequence 50 AACAGGGATCCTCTGCTGTG 239 GGTGGACTCTACCTCCATCG Sequence 50 GTGAGAAAGCGGAGAACACC 241 TTAGGGCGTTTCTTTGTGATG Sequence 50 AGCATATGGCCAGACAGGAC 483 AAAGCTGGCACAACGACAG Sequence 50 TGAAGAGGTCTCCCTCCAGA 410 GAGAGGAAGAACTGCCATTCA Sequence 50 GTCCTCCAGGAACAAGCAGA 487 ACGGCTGCTTCAACAAGTG Sequence 50 GAGCAGTGTGAGGACTGCAA 555 GTCCTCCTCCTTGGGTCTGT Sequence 50 TAGGCACATACAGCCCAGTG 202 CCTTGATAGCCTTCTCCACCT Sequence 50 GCATATCCAGTCCTCCTCCA 311 CCTGACACAGCCTCTCCTTC SEQ ID NO: 60 TCTCTCCCACGTGGATTCTC 987 GTGTCCCTGCAGGCTTCTTA SEQ ID NO: 61 AGGAGCACCACTGAGCTACC 682 TTGCTGCTTTGTTCTCAGGA SEQ ID NO: 62 AGAAGGACAGGTTACAAGAGCA 852 GCATGCAGAGTCTTCACAGG SEQ ID NO: 63 GTTGTCCAGGCCTCTCCACT 354 CCACTGCCAAACTTCCACTT SEQ ID NO: 64 AGCGTGACTGCAGAAGAAGA 311 AGAATCCAACTTCGCTGTTCA SEQ ID NO: 65 CACTGGCTCATTGCACAAAT 679 AGCCATGGGTCTTCTGTTGT SEQ ID NO: 66 TACTGCAGCCAAACAGATGG 442 AGCTCCTCTGAGGTGGTTCC

SEQ ID NO: Primer sequence Amplification Product Size (bp) SEQ ID NO: 67 CAAGGTTCCGGACCTACAC 723 GCTGTGGCGATTTCGTAAGT SEQ ID NO: 68 AGGACATGCGAGGACGACT 716 CGGATGTCGTAGCCATACAG SEQ ID NO: 69 CCTGTGATTCGTCTCATCCA 534 TGCAAGAGTGATTTATGCTCCT SEQ ID NO: 70 ACCTTTGGTCTTCGGGATG 524 TGTTTGTGAAACCTCCATGC Article 71 TTTCCAGGCAGTTGTGTGAG 330 CCTGGTGCTGCTTGTCATC SEQ ID NO: 72 GGCAATTCTGCTCCTAAACG 617 GGGCATTCCATCTCTTCACA SEQ ID NO: 73 GAGGCCATCAAGAAGCTCAT 615 CATGCTGCGCAGTGTCT Sequence 74 CAGTCATGAGAGGCACCTGA 528 GGAGGAAGAGGTTGGAGAGG Sequence 75 TGGTGGCCTTAAACTTCCAG 510 AGGTTCAAGTTTGCCACCAG SEQ ID NO: 76 TGCTGCTGATGAAGACACCT 738 GGGAACCTGAGAGCACAGAG Article 77 CAAACTGAACCAGCGTCTCA 515 AATGCGCTTCACCAAGTTCT SEQ ID NO: 78 GGTGAGGGTGCCAGATCTAA 932 TACACTGGCCTCGACAACTG SEQ ID NO: 79 ATTGCCTTCAGGATCAGGTG 427 TGCACAGCTGCATGAAATCT SEQ ID NO: 80 ATTGCCTTCAGGATCAGGTG 589 GATGCTGAACTGTGCCTGTG SEQ ID NO: 81 GGGATGGATGAAGATGGATG 707 CATTTCAGGATGCCGTGTC SEQ ID NO: 82 GCATGGCTGAGGGAGAAATA 961 TCACACCAGAGTCAGCCAAG SEQ.83 GAGATCCTGGAGGCTATGGA 316 ATCAATTCTCCGTGGAGGTG

SEQ ID NO: Primer sequence Amplification Product Size (bp) 84. GACCCTTGAATCCCTGTGAA 228 CATGTGCCTCTGCACAGTTT 84. AGCAGTTTGGGAAGCACTGT 508 TCATTCTGCAAACGTCATGG 84. ATCCAGGTCCTTGTGCCTTT 588 GAAATGGATGCTCGGTGACT 84. CGCATCGATCATCCTTTGTA 847 TAAAGTGCCCGTTCTGCTTT 84. GCTTCTGTTTGCTTGATGCTC 610 TCCATCATGGGTAATGTCCA 84. CATTGCCTCTCCTCGAAGTAA 216 TGACACGGAACTGCTGGTAG SEQ ID NO: 90 TGGCAGTGCCTGTAAATCAG 704 AATCATGCACCAGCAATGAG SEQ.91. CTTCTGAGGGACCAGGAAGG 234 TGTCCCATAATGGCTTGTGA SEQ ID NO: 92 CATTAATCGGTGGTGTGAGG 715 GCAGACCGTTCTTGTGGAAT SEQ ID NO: 93 TGCCCAGATCACGTAGAATG 402 GCCACAGAACTTCCTCAAGC SEQ ID NO: 94 GAGGTCACCGAGGTCTTGAG 732 ATCCTTCCGGAGCTCTTCAG SEQ ID NO: 95 TCAGGAAAGCCTTCACCACT 761 TCCTTGAGATTGGGATCCAT SEQ ID NO: 96 GAATTTGGTGGAGCCAAAGA 710 GAATGGCTTTCTGTGTCACG SEQ ID NO: 97 CGTGGCACTGATCCTAATCC 711 TCAGGATGTTGCTGATGGAA SEQ ID NO: 98 TACCTCTCTGCAGCGACAGC 642 AGAAAGGTGGTTTGCTCCTG SEQ ID NO: 99 GAAAGCAGGAACCACAGAGC 746 GCGATACCCATGATTTGGTC Sequence 100 GCTGGTAGCCGTTAGTCTGC 786 TTGGAAGTCTGTCCCAGGAG

SEQ ID NO: Primer sequence Amplification Product Size (bp) SEQ ID NO: 101 AAGGTTGGGCAAGCAAGTTT 626 GGCATCTTTGCACAGAGAAA SEQ ID NO: 102 GAGCTGGAGCAAACCTTGAG 637 CCTGGCTAGACAATCCCTGA SEQ ID NO: 103 CCACACTGCTGTTCTTGGTG 525 TCTCCTCTGTGAGCCATGTG SEQ ID NO: 104 TGCCATCCACCAAAGGTTAT 302 ACTGGCAGTAACAGCACACG SEQ ID NO: 105 GCCTCAAACTTTCCCAGTCA 420 CAGTCCTTCCCAAGTGGAGT SEQ ID NO: 106 AAGACTGCAACATGCAATGG 705 CTCCACTCTGCCTTCACCTC SEQ ID NO: 107 AGATGTGTGTGCCCAGCAG 323 AAGCTGTGGTGATGACGTTG SEQ ID NO: 108 CAGTTTGACGTGGACAAGGA 651 ATAATCTTCACGCGGTCGTT SEQ ID NO: 109 CACGTCTGATTTGGTTGTGG 856 ACGCGTTAATTTGGCGATAC SEQ ID NO: 110 CAACTCCGTCGGTTACTTCC 923 CCAGCTGAGACAGTTGGACA

Gene On847 Annotation and Bioinformatics Analysis

We have found 26 TCs that can be classified according to gene ontology index. Testicular-specific TCs were distributed in three branches of the GO index: molecular function, biological processes and cellular components (FIG. 3). Of the sequences distributed in biological processes, 51% encode proteins involved in physiological processes, 31% encode proteins involved in intracellular processes, and 15% encode proteins involved in development (FIG. 3A). ). Of the sequences categorized into molecular functional groups, 46% were involved in binding and 42% were involved in catalytic activity (FIG. 3B). Almost all of the TCs classified as cellular components (94%) were distributed within the cells (FIG. 3C).

The distribution of testicular-specific TCs and chicken total TCs in the biological process of the GO index and in the cellular component branch were similar. However, of the testicular-specific TCs annotated in the molecular function branch, 42% were classified as having catalytic activity, 46% were classified as being involved in binding, and these percentages were found in the percentage of total TCs in chickens (each 22% and 36%; higher than FIG. 4).

Of the 292 candidate TCs, 55 were not amplified by RT-PCR, and 46 of the unamplified (84%) were assembled into up to two ESTs. TCs assembled from more ESTs can provide more reliable sequence information in EST library screening.

The chromosomal distribution of testicular-specific genes was not significantly different from the distribution of total gene numbers on each chromosome (two-tailed chi-square test, P = 0.165). The number of testes-specific genes composed of exon alone was not high.

As described in detail above, the present invention provides a testicular-specific nucleotide sequence and a method for identifying testes cells using the same. Since the nucleotide sequence of the present invention is specifically expressed in the testis, it is not only very useful for identification of testicular cells, sex discrimination, segregation of sperm, infertility treatment or infertility and gene diagnosis (especially infertility diagnosis), It provides basic information for studying a set of mechanisms that govern sperm production.

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that such a specific technology is only a preferred embodiment, and the scope of the present invention is not limited thereto. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

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[13] X. Jin, E. Turcott, S. Englehardt, G.J. Mize, D. R. Morris, The two upstream open reading frames of oncogene mdm2 have different translational regulatory properties. J. Biol. Chem. 278 (2003) 2571625721.

[14] J.H. Shin, H.B. Kim, K.D. Song, B.K. Han, T.S. Park, D.K. Kim, J.Y. Han, A set of testis specific novel genes collected from a collection of Korean Native Chicken (KNC) ESTs, Anim. Genet. (2004) submitted.

[15] P. Lopez, R. Yaman, L.A. Lopez-Fernandez, F. Vidal, D. Puel, P. Clertant, F. Cuzin, A novel germ line-specific gene of the phosducin-like protein (PhLP) family. A meiotic function conserved from yeast to mice, J. Biol. Chem. 278 (2003) 1751-7.

[16] P. Kueng, Z. Nikolova, V. Djonov, A. Hemphill, V, Rohrbach, D. Boehlen, G. Zuercher, A.C. Andres, A. Ziemiecki, A novel family of serine / threonine kinases participating in spermiogenesis, J. Cell Biol. 139 (1997) 1851-9.

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[18] E. Mori, S. Kashiwabara, T. Baba, Y. Inagaki, T. Mori, Amino acid sequences of porcine Sp38 and proacrosin required for binding to the zona pellucida, Dev. Biol. 168 (1995) 575-83.

<110> Avicore Biotechnology Institute Inc.          Seoul National University Industry Foundation <120> Testis-Specific Nucleotide Sequences <160> 110 <170> KopatentIn 1.71 <210> 1 <211> 665 <212> DNA <213> Gallus gallus <400> 1 agaccatctg caagtagaca tcagtctagc agcacattcc ctgccagctc cttctctcca 60 cagcagcaac atgtctgggc tagaagaatc tttccgtaaa tttgcagtat atggtgacac 120 agctgccagt ggcaacaaca tgacagggaa aaacttctcc aagatgtgca aagagtgtgg 180 ggtgatggat ggaaaagctg tgaccagcac tgacattgac atcgtattta acaaagtcaa 240 gaccaagggc gcccgcacca tcaattttgt tgagttccag caggccatga aggagatctg 300 tgttaagcgt ttcaagggca aatcaccaga ggaagccctg caagctgtgt atggcctcat 360 tgaagggaag gagccaagca acgtgggcac cacaaaagtt gccaaagtag ctggggtcga 420 caggctgacg gacactagca aatacactgg cagtcacaaa gagcgctttg atgagagcgg 480 caaagggaaa ggacttgctg gccgtgagga cctgacagac aacagcggct acgttggtgc 540 ctataaggga gccggcactt atgataagac tcactaggac tgaagctact tagggaagca 600 aggatcttct tcctctaata cataggagaa gaggcaaata aaatctcatg caactgaaaa 660 aaaaa 665 <210> 2 <211> 867 <212> DNA <213> Gallus gallus <400> 2 ctgggttggt gagcacaggg tctcagcctg cagagaagaa ggctggagct gaagcccttc 60 ctcagggaag ctcggctgcg gtggagaggt ccttgcctga gtgcgtgggg ccacccagcc 120 actgacacct ctagactggg ctggtagagg tggagcaggc agaaaacacc agcaagggcc 180 agaaaataca tcaagagcag gcaagaaaag catacaagga agatcagcct tcacagcatg 240 gagagacctg tagaagcaag cagcatgccc ggaatggatg gggtctgcac tgcacctgag 300 cagaagaggg agcctgaggc atcgtgctct ggggagccct ccaggcgccg agaagcaaag 360 gtcaaacaga gtcgctcctc tcgggctggg ctgctcttcc ccgtgagccg catagagagg 420 cagctgcgca gaggccagtt tgctgagcgc tttggagcca atgcccctgt ctacctggct 480 gctgtgctgc agtgggtgac gcacaagacc atggacatgg ctgggaagat ttccaagaag 540 agcaaccaac agtacatctc tccatgccac ttgcagatgt ctataaagaa cagccctgta 600 ctcaagcacc ttctgggaag tgggcccaag caccgtggca gggctgtccc aaaaagccag 660 cgcgtggcct caacctccaa aacgaagaag accaagagga tgaagagaag ccacagacaa 720 cagactgcac ctgcccgtgc cactgctgct gtcactgtct agtgaaagca gaaactcctg 780 tcccattctg gcatggcaag ggctgtctgg tttgccaaat tacctgctat gatctaaaag 840 ttgaataaaa gcccttgcac taaaaaa 867 <210> 3 <211> 1448 <212> DNA <213> Gallus gallus <400> 3 cgcttgagct cggcgtgagt acgtggcttg ttgcacttgt gcatcgactg agtctgttcg 60 tcttcatccc gtcttgcgca gcgccttgta gcttttgctc gcggaagatc agagcaaagt 120 gacaaggaac ggctgcgggt tcgaggatct tcttctccct tgttctgatc tcttgccagc 180 ttgtcgcgcc tgctgtgatg ggcatcgcga gcagcgagat cagccgtggg cctgaccggg 240 cgttgacgga gcctgagaac caggggaagc ccttgctggt ccgtgaggag tcatctctta 300 atattccagc tattgctgct ggccatgtta ttaagagata cgttgcccag gcagcggatg 360 agctctcctt ggaggtggga gacctggtgt gcattactgc tatgccagca aaggagcaga 420 gcccctggtg gagaggcaag catggctttc aggttggatt tttccctggt gagtgcgtgg 480 agctcattag tggaaaactt cctgaggccc tcatcaattc agcaccaaag ccagtgccaa 540 agaagcgtgg caagctcctc agcttccttc gttccttcgt gaaggcccgc ccagaggaac 600 cgaagcagcg ggagatggag ctggagaagg agaaggaagg ggtgtttggc tgtgacctgg 660 gagagcatct tctccactct ggccgtgatg tcccccaggt cgtgcagagg tgcgctgagt 720 tcattgagca gcacggcgtg gtgcagggga tctaccgcct gtccggcgtg gcgtccaaga 780 tccagaagct gcgccatgac tttgagtcgg agcagattcc ggagctcacc gtccgggaca 840 ttcacagcgt gagctccctg tgcaagatgt acttcaggga gctgccgagc cctctgctga 900 ccgagcagct gcatggcaag ttctcggacg ctgtttgtgc cggtacagat gaggagcggt 960 tgctcagaat gcaggaggtc atccagcagc tgcctgcacc tcactacagg acgctggaat 1020 acctgatgag acacttggct tgtctcgctg ggagctgctc catcacaaac atgcccgcta 1080 agaacttagc gattgtgtgg gctcccaacc tcttcagatc ccagcagaac gaggctgcct 1140 gcgccagcgg aagagctgcc tgcgaggaac tgcagaggca gtcggacgtg gtggagttcc 1200 tcatcaacca caccgacgtc ctcttctgct ccagctccac gtcaggcatc ggagatggag 1260 caggtgagcg tcttcctgca tgagctttgt cttgatgaga caccttgatc aaatgcaggc 1320 cttctccagg attgttgaga tacgcttttg ctatcgaggg gccagcagac ttgtgggctt 1380 agatgagaat atggcaacct gtggctggat gcggagaatg tccaagccca aagttgagca 1440 tgagaaac 1448 <210> 4 <211> 912 <212> DNA <213> Gallus gallus <400> 4 tggaggcggg gccgttagaa ccgggtcccg ccccaaccgc cgcccagagc agcagtgcgg 60 ctgcggggag ccatgcagga cccaaatgaa gataccgaat ggaatgacgt actaagaaaa 120 tttgggattc ttcctccaaa agaagagcca aaagatgaaa ttgaagagat ggttttacgc 180 ttgcagaaag aagcggaagt gaaaccatat gagagaatga gtcttgaaga attaaaagaa 240 gctgaagatg actttgatga ggctgatagg aaggctattg agatgtacag gcagcaacgc 300 ttgcaagaat ggaaatgcct tcagaggagg caaaagtatg gggagctgag agagatcagc 360 ggagagcagt acgttaagga agttacaaat gctcctgagg atgtttgggt cataattcat 420 ctttatcggt caagcatccc cgtgtgcctt ctggtcaatg aacacctcag ccacttagcc 480 aggaagtttc cagaagccaa gtttgtcaaa gccgctgtga acagctgcat ccagaggtac 540 cacgacaggt gtttgcccac tatacttgta tacaaatctg gtgaaataaa agcccggttc 600 atcggggtgg ctgaatgcgg cggggcgtat cttaaagtgg aagagcttga gtggaagcta 660 gcagaagttg gagcaattga aagtgactta gaggaaaacc ccaagaagga cattataaat 720 atgatgacat tatcaatgca aaacatttct gttcatgaaa acacggatac aaaaagcagt 780 gacgtgctga aacctcgtat tacttgagga tgagtttccc atgtgggtaa tccgttcagg 840 ctcaaactag cacacatgta gcttcctact gcttttgaaa gtctgaacaa taaaaagggc 900 ttttgttccg ct 912 <210> 5 <211> 823 <212> DNA <213> Gallus gallus <400> 5 cggagcaagg acacggagat gccgggctct ccttcggcta ccgtctctgt tccgtcccac 60 acaaaacccc actgatctaa atggggagcc ctcagtgaca tcctgctccc tgcatgtgtc 120 actgaggctg cagaaggctt tgtcactgtc acctgaccca ggctgaggac aggttgcatc 180 ctccagtttg ccatcctgaa atcatgccgc ggcggctcag cgtctctgag agcatggaga 240 gggcctgcac tgagcagcag agcctggaag aggaggatga tgatgatgat gacaacgacc 300 acgaggagtt cagttacacc tgtcccttgg cacatcctcc accaccatgc atgagtttgg 360 gtgacactca gctgggagat gctgagttct catttgagcc cgggcaccct tgcatggtga 420 aagatgacgg agagaaaaac agaatatacg ccaaaaggtt ggcaaggcac gcgttggagc 480 tcaaagagaa tgctcaggag tttgaggaac cattttacaa agacacggag atgtccgcca 540 ccccattcga ggaggaggac agtggctgga ccaggaccta ccacctccct gtgcaccaaa 600 ggaaacacac gtctcgagct gccaacgtgt caccatgttc ctatgactcc taccagcatg 660 gcacgggtaa acacgtgggg acagacttgt cttccctgtg ggcccaaacc aggcgaccca 720 cacctctcca gcctggagca cgccagggga gcagagcctc acagtggacg aggagtcatt 780 ggaacccggg aggtccctcg cctgcgtgat cctacaggga gct 823 <210> 6 <211> 943 <212> DNA <213> Gallus gallus <400> 6 aatgatgaag gctgaccacc tagtgctcat gactcctgac agcagtactg aagagtaaat 60 ttgagaaacc atcatatgaa gatgagccag ataccagcac aggagctaaa tggaacgacc 120 gccgggagtc aaaacttggt tccttgtaga atctgtggaa gacagtttgc accagatgtt 180 ctgatgagac atgagcccat ttgcaagaaa gtcttcaaca agaagcgcaa gcccttcaat 240 tcttttaaac agaggctgca gggaacagat atcggaactg tgaagaggca gcccccactg 300 aagaatcagc cggtgaagaa atctaactgg aggcagcacc acgcggactt tattaatgcc 360 attcaatcag ccaaacaagt gacgaaagct atgcaagaag gccgtcctct tccacctccc 420 cccccaccaa gcatcaatcc agactatatt cagtgtccat tctgcttgag aagattcaac 480 gaggctgcag cagcaaagca catcaaattt tgtgaagaac aagctgctcg ccgtgccatt 540 gctgctaaga tcaccaaaca gcctatgggc aagcaaccag tgactcagag gaaaaaacca 600 actgtcacac ctctgttacc gcatgagaag agagtacaaa aggttgataa tgcagaaaaa 660 tctactccag agactcctcc tggaacactg caaaaaagca gaagaccttt gggtatatct 720 cctggaaaaa atctgctgca gaatttggac agcagacaca gaccagaaga tagagaggct 780 gcctgccagg gatgcattaa ggtgttttgc agaagcacta tgggccttaa cagtgtgatc 840 ctccagcacc tcttccaccc caagtgctgc agctcagtct gttgtgatgc agtggtaata 900 atacaatggg tatcatctcc aattaaaata ataataacaa tgg 943 <210> 7 <211> 769 <212> DNA <213> Gallus gallus <400> 7 ctgcactcca tggacagcac acagagagca ggcttcctcc tggccatgtg agcaacactg 60 cagatgggag agatgttcaa gaccacatcc gatgcactga taggacgaca gaaggacact 120 gaagctgagc acgacccgga ggagctcgat gtgcaaaaca gaagtactga agtgaccttt 180 gaaacaaagg actacaaagt gacagcaggc tgcattgctg ccagcagtga gtcgagtgga 240 acatccagtg atggccagga tgaggatgac caggatgaaa catgctttgc tgtttctctg 300 tctcccaaag gaatcaggac cagcaggaaa aggccaaaga taaagagcaa gcagtcaccg 360 gtgcagaaga aaatgagaat agtagaagtg gaagaagagc cattgctacc agtaaaggcg 420 gaggctgcag aaagagcagc tcaggaacag gagaacagag cagaagtagc acaggaagcg 480 acggggcagt gtgtattgtc tgacattatc atgctcgtga tactcatcct cgttgtattc 540 atggtcttca cgcacctcac tctggcagtg gtggtcacgg tctgctgctg agcttcaggc 600 tgggaagaga gagagagagg cagcagccac gtgtcagcag tgagtgcaga actggagaat 660 gcgtttcttt tctctccctg agatgtgcaa tgagctcttc tggtggccaa ttaaagggct 720 gggaggagag cgagataaaa aaaaaaacat ttttttttta tatttaaaa 769 <210> 8 <211> 1979 <212> DNA <213> Gallus gallus <400> 8 ccccggccgc cgggccatgg ccgggctccg gctcccggcc ccggaggtcg cccgggggag 60 cagcagtttc cgtggcggag gcgccgagct ccgctgcctg cgcgcgaatt tgcaagttgc 120 actgaagtaa gaccttgctg ctgcaagtag agctggttac catgaagctt gccggaattg 180 tctgcatcgg ggaacactat ggagcaattt tcaacatgga gaagaaactg ttgtgtgcat 240 ctggtcacag aggacattat ttcagcgaca gaaaactgcc agtgagtgag gcagcaaatc 300 cctggcacca accccctagc tctggagtga ggggaaatgc tggagcactg aaggataaag 360 ccatgagggc agtggtgacg ggaggtggag gctacttcgg atacaagctc ggatgtgctc 420 ttgccagctc aggagcttca gttgttctgt atgacataca caagcctatc tgggaaattc 480 ctaatggagt agtgtgcatc caggcagatg tcagggatta tgatgctgta tttgcagcct 540 gtgaaggggc tgactgtgtg tttcatgtag cttcatatgg aatgtcagga agagagcaat 600 tgcacagaga agagattgaa acagtaaaca tcaacgggac aagattcatc attgacgctt 660 gcaaacaaag gaacatcact agattgatat acaccagtac agtaaatgtg gtgtttggag 720 ggcttcctat tgaagatggt gatgaggaaa ctgtgccata ttttcctata gaaaagcacg 780 ttgatcatta ttccagaacc aaatcaattg cagaacaaat ggtactagca gctaatggaa 840 ctccactagc aggaggtgga gtactctaca catctgttct tcgcccacca ggaatttacg 900 gaccagaaga gcaaagacac ctgccaaggc tagcaaagaa tattgagaga gggatactta 960 gcttcaagtt tggggatcct tctgctaaaa tgaactgggt gcatgtagag aatcttgttc 1020 aagctcaaat tctggctgct gaagctctca ctcctgagaa gaactacata gctagtggtc 1080 aggtgtattt cattcatgat ggtgaaaaat tcaacctttt tgaatggtta gctccacttt 1140 ttgaaagatt aggttgcagt aagccatgga tacctattcc tacttcctta gtttatgcat 1200 cagccacagt aatggagcat cttcatctga tactgaaacc attggttgag ctgtcacctc 1260 tgctgaccag aaatgaggtg cagaacatct ccacaacgca caccttccgc attgacaagg 1320 cccgcaggca gcctgggcta cagccctgag aagttcacct tcgctgattc agtggagcat 1380 tacatcaaaa ccagagcaga ggccccggag cgagcacggc ttccccagag tgatgctttt 1440 gttccttgcg attttaagtt tgatttttct ttctttaaga tttgatgacc tttcagtttt 1500 gcactttttt aaggaagcgc agcactgact cacacagact gcactgctcc catggaacat 1560 ccccagctcc acaacaccca cgggccccag tgcagaacgc gggcacctat tttagatcaa 1620 aaacagagca gacctaaaca ccaccaccag cactgtaaac agttacagcc aaagtccatc 1680 accgagacac gctgaagtca cacatctttc caagctgact gctgaagtgc acgaggagct 1740 gcagaaggaa cacagggcac agcccacagt gcttcaccga tggagaaaag caggaaagca 1800 ggagatggag tcggagcagc agctcgtggc cgtagggagc tgaggaacgt gcatgggaag 1860 cgcgtggatg gctcacggct gtgaatcaca cctgtgggct gcgcagagct cacacggtgc 1920 aaattttcat tgtgcattgg ctgtgtcact gcatatgagg gaaaataaaa gggaacgtg 1979 <210> 9 <211> 961 <212> DNA <213> Gallus gallus <400> 9 ggcggctgct ggcggacggt ttgggcggga aggggcgagt gagggcgccg ccatcttgac 60 gctgaggtgt tccagagact gtctgttgga caaagggttg ccgaggagct gctctgaatg 120 cagcaatgct gtccctctgg agcagggata acagagagtt ttgtgtccag caacacagct 180 tcatgagaca cccttcttgc tatctttggt ggtattattt gcaaaatgag cccttgccct 240 ctggcaccag tcagaaacca gatgagaggg aataccatga agacaaggaa ggcttcggga 300 ggatcagagg ggaaattctg ttttggtgct gcgctggcat tttgattatc tgccttctct 360 gctgtgctta cactcgaatc ttccagaaag ctcaagataa aaggttatgt tctgacaaaa 420 gggagaatgc ttccaaagct cccagtagcc ctgtctgcaa atattacagc atgttcagtg 480 cctggctctt tcttagtaag atttataatg ggataaagaa aaagtgcatc caacagcctt 540 acatctctac ctgtcactct gggacaaccg cagcgacgtg ttcccgtgat caaaggcaag 600 agaagcagtt ctcaaagctc agacaggaga aagcctccaa cattgacaat ggcaactcag 660 atagctgggc acctgtgtgg aaggccgatg agatgcagtc catcccccgt gaagtgagcc 720 ataaacagca tccggcacaa aagcaaaggg aggcagagag cttttggatc agggaacagc 780 cctgtggcca ttgtaaagct gagaggctcc agcagtggct gcaccagcac tttattaagc 840 cttctgagag aaatcttcat tagatgagga cgtctggcaa gtttgtccaa gaggaaaata 900 gctgcaaagc tgacctcctg agtcctgtcc acccgtggct ctggttctgc tcctgctttg 960 c 961 <210> 10 <211> 830 <212> DNA <213> Gallus gallus <400> 10 cagaagggat tagcattcac ttgtcctctc tttctttagg tatactccgc cagctaagca 60 atggatttct gtgagtgaaa caaggcaaaa tgagtagtga gcttgatttt aaaaagatta 120 caccaagtca gaaattcatc atgggtgacc tccagagact tcttaaccaa ggagaatatg 180 ctctttttgc ctctgttccc atgtttgaaa gcaggtttgt tcagattaac caaagagggg 240 aactgattaa tctccataat cgaccaacct gcgtgaccat cggcgtctgt gctgccagtt 300 caccaatgcc cagtggaatg ctgctagcac acaaggtgcc tgtgtcccca caggaaataa 360 tggcaaatct ccagaaattc tcagagcatc catcccaagt ggaacagcta gcgcttagca 420 ggttcttacc cttgaagttt gtagaactct ctgttcacag cacagataag aaacaactca 480 tgttaaaatt agtaaatggc cgttcctatt atctagagct ctgtgctcca ccagaccaac 540 aacagcaact attccaccac tggttgaagc taatctctct cctgaaacgt ccagaagata 600 ctagcaatac caacgtcaac ataatatgta aggattctgg cacaaatcat gagaaagctc 660 ccagccccaa caacccacca gacaacagca acaactcgca aacatgccta acaccaaaac 720 agaggaagaa actgttactg aacaaacctc ctccaacagg ttccccactc aggtagcgta 780 ggtcccacag aaaagagtgg aaaggggaag ggcaaccttc agcagctccc 830 <210> 11 <211> 440 <212> DNA <213> Gallus gallus <400> 11 acaacaccga gaaagggggg aggggggcga tggctgcgtg gggcgggggg cgaaccccgg 60 ggggtcacgc ggccaccgtg ctgcgatgct acgacatcct ccgggaggag atccaggctc 120 tgctggccga gaacgaagag ctggcgcgga tcgtcgcctc catccggcag cagcgacgcc 180 acctccacct cacctccacc cctcaggatt actccggagc gccccctttc ggcagcggga 240 cgtggagcag cgatccgttg tacgctgacg acgggacgtc gccttcgtcg tcgtcgtggt 300 ggagaagcgg taccggcgga ggtccgtggg cggcgctgag accgaacaga acttccgaca 360 tctcgtccga gaggctcctg gcgcgaggag ccaatgactc cgctcccgcc gctctcccct 420 caccgccgtg cctccaccgc 440 <210> 12 <211> 832 <212> DNA <213> Gallus gallus <400> 12 ctgggtgggg tggcatgtca gtctgccatt tgtccctcag gccaccacag atgaagccgc 60 gttggcagag cacagcaaga cagctgggtg tcagcatgtg gcaggcgagg cacagggctc 120 tctcctggcc ccacttgcca aatatcaccc ttgttacaca gtggagccct gacggtagag 180 gaggctgtcc cttgcaatgc ttgtgcagcc acatcactct gcaagccaca gccaacttga 240 ggcagcactg taatagtaga cgtgacaggc aacggcaatg gcggaagaat gtactcaacc 300 tgattggctt aagggttggc aaaggtagaa gatggccaag acaggagaaa caaaaattta 360 acagaaaaac aaagcaagaa aaaccagagg gagagatgca ccacatccac gcatcttcat 420 caaaaggcag tagtgggcag aaacagctgt gcaaatctcc tgcacagaga tcatggcaca 480 agaggcctgg ggtgacacgc cagcagcaca atggaaggaa gaggcggagg aatagagtaa 540 agtatcacca ccattccaag ccacttcacc aagggtcagg tgggcatcgc agccccaaag 600 agtcccaaat gcacagccct gccactggga gatgggcagc cccaggcctg ccattttgcc 660 cacaactgca actcctcctt ggggttgcag cagccctgtg agtcaccaat gggcagcaac 720 aaactcagag aagagaaaag caagagcaga aagggggaaa gttccctgct atgtaacatg 780 aaagatcgca gctgcaataa aggtttccat gtgcctcttt atctgctgca ag 832 <210> 13 <211> 697 <212> DNA <213> Gallus gallus <400> 13 gctgtgatgg actgaagttt attactggta gctgtgccta cactgtgtcc cctcagcctg 60 ccggctcagc tctcggcctt catacagcgc ccagcaggtg tgcagggcgg gcatggggct 120 gcggtcgatg gcggggttgt tgcactgcac tgttgggagc cacgggtagg cctcagccct 180 gggctcgggc tccctcaggg caacagcggg ctgctccttg acacaggcct gcatatggag 240 ccgggcgctc tgcagcaagc aggtgttgca ggcctcgcag cactcgggct ccacgggggg 300 ggcactggag gctcccagct cctcccgcag ctcctcctgc agctccagct cctcatccct 360 ggagaaggtg gcgtggctga tggagagtgn nnnnnnnnnn nnncggtcca cctcgccgta 420 gtcctgcagt gaagtggctg ccagctgcgg ggtgacgcag aggtggcagg agctgcagga 480 tatggcctcc ccaggcctgg acaccatgca gagggagctg agacatctca atgtgacagc 540 ggctcgccgc gggtcacggg gttctgttct actgccaggc ttcaccatgg tgatgacgtg 600 ggtacatcac tgccaggagg acgcctttgc ctgcgctgct ctgctatgcc ctagccctgc 660 tgcaggccag cattgcagga cgtgtgcgct gcctggt 697 <210> 14 <211> 829 <212> DNA <213> Gallus gallus <400> 14 ccgcgtcggt ggcgacgaag ccgggcgcga tgggctgccg tgcgcctccg ggagcgatgg 60 tgctgctgct gcccctcgcg gtgctgctgg ccgtctgccg gcccgggcac ggcttctccg 120 gcacatgcga cacctgcggg ctccggccca tggcttatca ctacaacaac atgcgcatcg 180 tgggcggcac ggaagccctg cacgggtcct ggccgtggat tgtcagcatc cagaatcccc 240 atgtttcagg cacgggacac atgtgtggag gatccctcat caccccgcag tgggtgctgt 300 cggcagcgca ctgcttcggc caccccagct acatcctgga gtcgcgcgtg gtgatcggtg 360 ccaacgattt gactcacctg ggccaggagg tggaagtgca caccatcagg agggcgatac 420 ttcacgagta cttcaacaac aggacaatga tcaacgacat cgcgctgctt gagctggacc 480 gccccgtcca ctgcagctac tacatccagc tcgcctgcgt gcccgacgcc tcgctgcgag 540 tgtcagagct cacagactgc tatgtcagtg gctggggaca cacggggatg agatctgtag 600 ttcctgtcca aacggccgag atcctgcagg aggccaaggt ccagctcctt gacctccacc 660 tctgcaacag cagccgctgg tatgctgggg ctgtccatac ccacaacgtg tgtgctggta 720 ccccgcaggg cgccatcgac acctgccagg ccgatagtgg tggccctctg atgtgcagag 780 acaacaatgc tgactacttc tggcttgtgg ggtgaccagc tgggggaaa 829 <210> 15 <211> 1056 <212> DNA <213> Gallus gallus <400> 15 cttgttgcac ttgtgcatcg agtgagtctg ttcgtcttca gcccgtcttg cgcagcgtct 60 tgtagctttt gctcgcggaa gatcagagca aagtgacaag gaccggctgc gggtttgagg 120 atcttcttct cccttgttct gatctcttgc cagcttgtcg agcctgctgt aatgggcatc 180 gcgagcagca agatcagctg tgggcctgac ctggcatgga cagagcctga gaaccagggg 240 aagcccttgc tggtgcatga ggagccatct cttaatattc cggctattgc tgctggccat 300 gttattaaga gatacgttgc ccaggcagcg gatgagctct ccttggaggt gggagacctg 360 gtgtgcattc ttgctatgcc agcaaaggag ctgagcccct ggtggagagg caagcgtggc 420 ttccaggtgg gatttttccc tggtgagtgc gtggagctca tcaacggaaa aattcctgag 480 gccctcatcg attcagcacc aaagccagtg ccaaagaagc gcggcaagct catcagcttc 540 cttcgttcct tcgcgaaggc ccgcccaaag gaaccgaagc agcgggagat ggcgaaggag 600 aaggaagggg tgtttggctg tgacctggga gagcatcttc tccactctgg ccgtgacgtc 660 ccccaggtcc tgcagagctg cgctgagttc attgagcagc acggcgtggt gcaggggatc 720 taccgcctgt ccggcgtggc gtccaagatc cagaagctac gccacgaatt tgagtcggag 780 cagattccgg agctaagcgt ccgtgacgtt cacagcgtga gctccctgtg caagatgtac 840 ttcagggagc tcccgacccc tctgctgacc gagcagctgt atggcaagtt ctcggatgct 900 gtttgtgccg ctacagatga ggagcggttg ctcacaatgc aggaggtcat ccagcagctg 960 cccgcgcctc actacaggac actggagtac ctggtgagac acttggcttg tctcgctggg 1020 agctgctctg ccacaaagat gcccgctaag aactta 1056 <210> 16 <211> 770 <212> DNA <213> Gallus gallus <400> 16 atacggccca gccactgcag tgtggggctg gcaggtgaca gagaacaggg aacacgacac 60 aggacactgg agacagggct ggccatgccc cggctcaggc tagtcctgct ccttgtactc 120 acgggaggaa ggaggagcag cagtggccac cgctgccaca ggacgaaggc gcgggctatg 180 acagccgcgc tgcacctcca taggcacagg ctgtcccgca tcaccccctg cctctacctc 240 agcgatggtg tggctgcagg caacgcacag ctcctggctg ccaaccacat caccaccgtc 300 atcaacgtct cccttgagtt ggccaacatg ctccaccccg gcattgagta cctacgcatc 360 ccggtggctg acagccccac tgcccgcatc tctgcctgct tcaattctgc tgccgacttg 420 atccgcagtg tgggtgagcg tgggggccgc acgctgctgc actgcgcagc tggggtgagc 480 cgctcggcca ccgtctgcat agcctacctg atgaagcacc acgccatgtc actggccagt 540 gcccatgctt gggtccggtc gtgccgaccc atcatccggc ccaacaacgg cttctggcgg 600 cagctcatcc actacgagta tttgctgttc ggcatcaaca ccgtccacat ggtccactcg 660 ccctttggga tgatacctga catttatgaa agggaagcga ggacgctgat gttctgagtg 720 tggcccaaat gggaaccagg ggaagtagaa agcggagctg cagcaaagtt 770 <210> 17 <211> 520 <212> DNA <213> Gallus gallus <400> 17 tcagcgcatc cctgctgggc tctctgcgcc ccactcgtgt ttttggcacg cgtggctgac 60 caatcgcagt gcctcgtcgg aatacagccc catagagccc cataggacct ctgggggacc 120 ccccaccccg ggaaggagga cggggggacc ccatggctgc gcccaccgag aggcactgcc 180 agcagcagag gaagaggagg aggaggcaga agttccaccg cgtccaatgg agcccttcgt 240 cccgaaggcg gcgatggagg tcatcccaga ggggacccgg caacgggatg aggacggaac 300 cccataggag gaacgcgtcc cgtcggtccg agctgctgac acccacatcc cggcggatgt 360 ccatcacggc ccggagggtg atgtgctcct tcatggccac cttctccagg ggtttggtca 420 gcgatgcgga tcagcgccgg cggcagcgcc gggggtcctt cattgggaca ttggagctga 480 aggcggcggt ggatgcggcg atggagggca gagtggggct 520 <210> 18 <211> 1009 <212> DNA <213> Gallus gallus <400> 18 acgtttccaa atcatttatt tcctgaagta tcagactacc catccccatc attttacaca 60 acacaagata aggatacgaa caaagtgaat ttatttacaa agctatttga caactacact 120 atgctatgct aatacatttt agtgtcaact aactctctat gctatctact ccattgtttg 180 ctggctcagt ctctgcattc agtctttgta tgtaaggtca cacgggcgca gtcatctggg 240 ctctcctctt ccgtccgttt cctccgtggc tgttccggag cagctgagca tcgtctcctt 300 gaaggtcgga tgacaggaga agactccctc ggcgcaaaag gtgcgtcctc ttctccccga 360 cgatcttcca gcaggtcacg cttccaagag ccgctgttct tcaactccct tgcagttcac 420 gtacttcctg tgagcagaag gcgcgtcttc ttctccgtga tgctctgctg gcaggacggg 480 tctctgcaga gctgctgttc ttctgctggt gatggagcag catcaggcgt tggtcttcat 540 tgtactgagg ggagagtggt gtcccaggat gcagtggctc atctgaaaca gagattcttt 600 ttagtaacgt tctatatcat actctataaa ctaactgtct cccagtcacc cgtcattcta 660 tcctgcatcc ccctatcagc tatctggcta tgatctaact acatcactac taactgttga 720 ttgcatgcta actactttgc tatccataaa ctacctctct accaataacc cctagctatc 780 tgctaactgt tcactacctg tatacaacca taagctatct atctcactat ctacgtcgct 840 accaactatt atgctaccca ctaactatct acgtcgctat caactatcta ctaccatgct 900 acccactatc tatctataca acaactatct ctatatcctc ttcttcatca tctatttcta 960 ccggcccctg atcccccccg gggctgctct cctcccgtcc ctcacactg 1009 <210> 19 <211> 1465 <212> DNA <213> Gallus gallus <400> 19 atctcaaaag gagtgcccga atgcaaagag gctgaaggat gctcagtggc atgaacagct 60 gttcaggccc agcagtgaaa tcagaggggc cattcccact ggggccgttc ccacggcaca 120 tctggatcca cgacaacacg ccccaggacg ggctggacaa ggcctgccat gagatctgga 180 aacgcacgca gagcctcccc tgggagctgc agcccatcgt ctgcacggac ccaccgggtc 240 ccccatggag caggaatggt gcccacagtg gagactggtt gctaaatgaa acagagggac 300 tcgaaagtct atacagagca gtgattggaa acagagagag agagatgaaa ttaagagctc 360 tctgtgatgc tcagctttct gctaaatcca ccatcaccaa ccttctgcat tcagcaagtt 420 cttccacgag ctccaaaggg ctgggggaca gcagcacaac aggtgctggc agcacagagg 480 gcagcaagct ggggatgcca ttgctgctga aacacacaga cactgccaaa ggcccagaca 540 ggcaggtcat ggctgaggaa agcaagacag tgaaagattt cctccagaat ggcatgttca 600 gtgctgcaaa gaatagcaca gctgtgccag ctccgatggc agaatgtcaa actgcaggac 660 agaagcagca gctctcaaca gttgccaaga tttgctccag acctgactcc agtgcagcca 720 caaaggcact gcagaatcca gtaactactg cagtgttgga taaaaaaatg gttctgtaca 780 acgccagcgc ttctactcca cgcttttctg ctactacaac caccacaacc ttaaaccagc 840 caatgtggcc aagtctgagc ttccctccac ttcctctatt tccaaaccag ccaaacttct 900 caccttttca gggtccgtac cagcgagcaa ggatcccttt ccagcaagct ctgcaccctt 960 cctttggatg ctattcaaga cagggggctc cattcagccc acagatgttc cgctcaccct 1020 acactcctct gctgaattac atccccctgg ttcagcccgg cttcccacac cagagggccc 1080 cacttcagct gcccaccagc atccaggacc tgccagcagc acgcgatggg atgcggttcc 1140 cgtttcctcc tccctatggg tttggtgctg cacccggagg agctgtgagg gccacacacc 1200 attacccctc caatgggagc tgcatcaact tttaagagaa ccagttctcc tactgtaata 1260 gcaccagatt tttgtgtaaa tatcagaagc cctaggatcc cataagaact ttgtgttttc 1320 ccagagagaa gagtctatct tgaatagaat tccacatgac ttccatgtta agaaacagct 1380 gcatgacaaa tcagaaataa tttcctttca ggatgacaga aaaagggcct ttttcctttt 1440 ggagaacaaa gacgtaataa aaaaa 1465 <210> 20 <211> 1128 <212> DNA <213> Gallus gallus <400> 20 aactgtgtgc aaacatattt aatttgcctt taaactagtt atttatgaat cttgcatata 60 tcacttttgt tagactgatg gctcaggact gcatcccagg tgacaccgac gctttgcaga 120 acagagctca gccagctgcc tgaagcccgg tgagaggagc atccatgggc tcatggtttt 180 gggtgttggt cagcaaggag taaccacggt gtgcggggag ccctgctgtt ctgctcctgg 240 aaggcccaaa cccctgagct gttgcttcag gtaagatgct ttttacaccc gtactgtagt 300 agtgctggtt aatgctaact ggttacattt aatgtatttc ctgttattgc aaatccagca 360 ttgaattgta agacaacttt ttgtatcact gaggcacagc tgtcacgctt ctgtcagtct 420 ttaccgagcc tgtttatgct ggctgttgtt ggagttcctt gtgccctacc ataattgcaa 480 gtgtgtcttt aaaggattaa aatttaatat gaacattcag ccatggcaca gctgtgatgg 540 aaaaggtaag agtgctccaa gttcaatttg gttttgcctg gagtggataa ccgtaccgtc 600 ctgcagctcc cgaccccccg acctgctgcc caccgggcac agcgcgctgc tgggcagggc 660 agggcagggc agggcagggc agggaccccg cagcccactt ctgggcgatc gtttcctctt 720 tgggacttgc cgctgttccg caggtgtttc caatgctgtg cgtctctccg agctatgtcc 780 cagctatgac tttccggaca gtgaccgagg gatgccgttt tgttcctgag cagcccttcc 840 agcccgtagc ccggggcgga cggagcccgg ccaccggcag cgccttcccg cagcgggccg 900 gagatgtgag caggtgagcg agagccaccg gcagctgctg ccgcggggtc ccggggatgc 960 tgcggggctc agggaatact gctggaggcc acgggacccc aggggtgcta cgggacgagc 1020 gcgatcccac gggagggccg ggggggtccc aaaggtgctg cgagacgagc gtgaccccgg 1080 ggtgctgcgg gaccgccggg gccgccgcag cgactgtgcc cgccgggg 1128 <210> 21 <211> 698 <212> DNA <213> Gallus gallus <400> 21 acagaattca aggatctggc tgccagggac cgcacaggtc agctgggcaa aagagcttgg 60 agaattgtct tcatataaga gcctgcaaag gacttcctga agcagacaga gaggatcttg 120 cttcacatca ccacctggcc tgtggtcatg tcactttcaa ggtggtatct caggcttttg 180 gaacttgcta gatcatctgt cttccttgcc tacacggtaa cggtatattt caagaacatg 240 acaagccacc taatcacaga tttacaagag aaggtgacag cattacagaa ggaaaaccag 300 agttttagga gatggaaaga gatcacatta gaaaatctca actcattttg ggaagggaat 360 ggagatatga tctcagactt ccaagacaag accaaggctg cttggaaaaa gaacatgact 420 ctctggaaaa aaaacatgac cttctggaac aatgtcaaca tcctactaaa ggatgaagag 480 gaaatctggg gaaaagatct agtcaaatgg aaagcagagc tggacctttg gaagaaggag 540 ctggaccttt ggggagagga acaagaattc cttggaaagt gaaaacattc acagaagaaa 600 acaaaactgt actggtcaaa aatcaaagat gaggcactcg tgcttagaat gatttctgcc 660 ttcttacact aataaaacct tgactgctct ctgaaatg 698 <210> 22 <211> 959 <212> DNA <213> Gallus gallus <400> 22 tgtcaacaaa caggctcaag gaaggaaaac acctggcaac agaaaagatt tgtcctccta 60 agcccctctc tctgttgttt ttccactgga taggctcagc cttttagtct ttccctgtta 120 tggctgaatg ttttggcagg aatcaaagca gcagcagcgg aagtgtctcc atggcccatg 180 aggatggaga ggcagtggtg gccaaccaca caagtgatgc ggagatgggc aaagataccg 240 aagatccaac aaaaggcaca cagacactct gtgggagccc tgaggagaag agagagaagg 300 ctctgctacg ggcacgcctg gaagagcagc atcgccttat ctgtatcctg aagaaaaaaa 360 cagatgatgc acgcaaacgc tgcaagagcc tggagcagct caacatggaa ctggagaaac 420 tgaggctaga ggatgctgtg aaactaaaaa ctcagaccca gcggattcag tacttggagg 480 atttggccaa caaccaagaa aaaatgatcc actccaagga tgagcaagag aaacagaaca 540 cacagttgaa ggaggaaaac cagcgcctgc agcaggagaa caaaaccctc ttcagccaag 600 cagcgaagga caacgaggct gaaatgctcc agcttgctgc ccaggccaga gagctcttgc 660 agcagctgga tactctacat gagaaatgtg cctgggagag atccagagcc cagcagctag 720 aaaaagagct cctggaagct cagagccagc aagcacatgc ctgtaccttt ggaggttgac 780 ttgctaagac aacagctgca gcgcctacag gaggagcacc aacaaatggt ggaacagcta 840 gagcatggag aaagccagca gaaggctcat gatgccgagc tgcaagccaa gctggagagg 900 gcaaatgagg agaaagagca gctactgaac tggccgcaaa aggggcaaac tctgcagaa 959 <210> 23 <211> 851 <212> DNA <213> Gallus gallus <400> 23 cagaggacag tgccggccgc ttggggcggc cgggtgtgct gaggcgccgg ttccacgccg 60 ctcggtaccc agaggggcct ggcggtgttg actgtttatg gagtgacagt aaagcacata 120 aacagagata cttctacctt ttagagtggt acactgtcac ccagccatgc ctacagaagg 180 aaaaagtgat atggagagga ctggcctctt cagtgaaatg agctatgtta ccattggaga 240 taaatatgta tcaccttata tgcgcccttt taacgaagct gcaagcaaga acagacagat 300 gctatctgag gggagcaaaa cattgtcagc tcttcaggca ggttattttg accctcagtt 360 taagaggatt ttcaatggtg aagcctactc aaaccctgtt caactaagga gacgttacag 420 attggcagaa tcaaagaaaa atttgggcaa agccttcctt cccagtaatg gagataaatt 480 gccaagtgga ctaggcagct attacggaac cataggaggt ccatttgaat acttcagtgc 540 acaactgaaa gccagagaaa gatacattcc tcctggaaag aatttttata ctagtccagg 600 aaaaaaagga actggatatg gttatgcaaa tcttaccata ggtgaacaat atgcacatga 660 agcggatggc tatgaattaa aaaaaataat tgcgaagaaa gcacgagagg aacacaagcg 720 tttggttaaa ggagggcctt tcaaatctaa tatatacccc ctggagtatt ttgacatgaa 780 tccctatttc agtgacaaac ctttgccacc tgtgaagaac caccaccgga gaagacaatt 840 gcaaaacctt t 851 <210> 24 <211> 1157 <212> DNA <213> Gallus gallus <400> 24 cgggcaggga gggcggcggg cgggagccgg ggcgggatgg gggcgcggaa gtgagcgggc 60 cgaggagctt tgctgcggcc acggagtctg agggtacgga gggagcggcg ggaggagcgt 120 agcaggttaa caagactttc agtaatatgg agaagggctc agaagtattt caggaacagg 180 ctctcactaa agcctcttca tgtgaagctg aacttcagga tttagtgcat caagttgaca 240 tcatagtaaa cagcaagaaa gtagaatggg aaaggaagat gaaagcttta gaagcacaga 300 tggatatcca gcatcaagaa ttagcaagta cccaaagcaa actggatcaa aaaagtcaag 360 aggtggaact actttgtcag caactggaaa acttacagaa aactaactgt gaaatggctc 420 agaattacaa aactcagctt tgtgcgctta aatctcaatt ttcgaagttg acacatagtt 480 acgaaaagtt attaaaacaa aaaaatcacc acagtagaga aaaatgtaaa gaaagcccgg 540 agacatcatt cgaactgagc aatttaaaca agaaactgga ggaatttaaa gcaaaatcaa 600 gagaatggga taagaaaggg acaatctgcc aaaatcatct catttattta gatgctcagc 660 aaaaaatatt gtctgagaaa tgtaatttgt ttcagataaa caataagaaa caaaaccagg 720 aagagactgt taaatatggc cagcctgaaa gagaaatgat gggttaattg ttgaaaagct 780 aagggcagca gtgagtgaaa tgacagtaag caggaataga ctagaagagg aaaatctgaa 840 actccgtgag gatctaaaaa tggtccaaaa ccagtgccag aacatggagg caagcttttc 900 agagatgaga aatgagctgc aatcacggga tgctctctgg agaaagatac aactggaatg 960 ccaacagttg cgtgcagaat tactgaaagt cagagagcat aaggataagc agaaacgcat 1020 cgatcatcct ttgtacaact tactgagcaa tcggaaaata aaatgctgat tacaggcaca 1080 gagtcaagaa gccacgggaa gtgctaagca gtgctgcgtg cagcagtggg gcatgccact 1140 gtggaataca tatggct 1157 <210> 25 <211> 987 <212> DNA <213> Gallus gallus <400> 25 ctgttgccga ggtaacggcg agcggcgtta gcaaccgccc tccccccgtt cggctccatc 60 agcgccgccg gacccccgcc atgcccggcc cccgagagcg cggtgccccc gcccgcggct 120 ccaacgcttt cccgtggcgg ggggtgggcc ccgagcagag gggccggctg ccccccgtgc 180 ccgccctgaa tcgggccccg gggagcctcc agctgggagc tcaggacagg ggtccctcct 240 gcacgccggt gcagcgggcg ctgctggcgc tcaccccgac gctggagaag ctgcaggagg 300 tgaagggccg cgtgctggag gccctggagc gggggctgag ccagcagagc cgcgcgcgtt 360 ccaccatgca gatgctgccc accttcatct gctccacgcc cgacggcacc gagaacggcg 420 acgtgctggt ggcggagctg tgccagaacc acgtccgcac cctctgggtt accctcgcgg 480 gcgacggaaa ccagaaccca cagctgatgt accgcgtctt tgagacgccg ggggacgtcc 540 tgcagggctc cggggaagcg ctctttgact tcatcgcaca atgcgtgaag agtttcctgg 600 aggaaatcgg caaccctcag caccgcctgc ccctgggctt cgtcttcccg ttcagctgca 660 ggcagacggg gctggacaag gcggaactca tctcctggtc caagggcttc caatgcagcg 720 acgtggaggg gaaagacgtg gtgcaactgc tgcaggcggc catcagcaag caggagctcc 780 accacggggg atgttgtggc cctggtgaac aacacggtgg gcaccatgga tgacgtgcag 840 cacaccggag aaaccctggg agatcgcctt ggttgtgggt tcgggcacca actgctgctt 900 atggccgagt cagacctctt ggagatcgcg ggggtgacca gcgggcggat gtgtgtcaac 960 agtgagtggg gctgcctggg ggatgac 987 <210> 26 <211> 1172 <212> DNA <213> Gallus gallus <400> 26 ccgaggctgt gcagcactgc agtcccgcga ggatcgcaga gcaggcgagt ggggaagagg 60 tgcttgtgtc ctgggaggag agcaagctgt tccctgtgtc tgtcactgga gtgaaggtgc 120 caaaatgctg gcccagctgg gcaggcaacg cgttgccgaa gagaggggaa ggaggactcc 180 tgtcctgctt ctagcagtcc tttctgctgg agccttcctc tggataatga caacttggaa 240 aaccaacgtc ctgcaggtgt cagcaggacc tgtgcatgag ctcccgtgtc tggggaactc 300 accgctctgc ctggagctga aaatgaaaac tattcagttc tcggccagaa aggacctcat 360 tctagatgcc gtgtttcaca gcctgaaaga caatgggatc gacggattga agagagagga 420 aattctgcag ctgacaagag aggcagttga gaaggtgatg aaacacgaca cctggctgcc 480 cagttgggct ctaaaaacca tgggtgccac tattgatgtg gagaggacat ccaacagtta 540 tcggggggaa cccgctggct ttctccattc ttttcctccg caaagcctcc tgagacgatc 600 ttgcaggtat ggcaggagaa atggtggctg tgggttcact gccttgcctt ctggctgcag 660 gagcaagcgc acagcaattt cctttggccc agctgcactg ctcagcccac actcttcccc 720 atcttactga caccagcgtt gggaaggtga tggggtgaca gatgcttggg agcgccgccc 780 cgccagctct gggcaagcag agaaaggcag ttgactccag gcctattgtg caaaccttta 840 catattcccc tcctgtccct tgagtagcgt tccattacga ggagaatggc agcccacaga 900 gaaggcaagg gtctgtgctc cttgccgaac agccggctct ggcggctcct tgccccaccg 960 ctgatggacg cgtctttctc ctttcagcct gatatttctg ctggcaactg ctgggctttc 1020 caaggatctc cgggcccacg tggtcatccg gctgccggag aaaatctggc ccacggcttt 1080 taccctctgg catatctccc aaggcagtct ctcccttggg ggggaagtca gcactgcccc 1140 agagaatttt gttgtctcgg gaatggatga gg 1172 <210> 27 <211> 652 <212> DNA <213> Gallus gallus <400> 27 caaggaagtc aacgccagta gcatatgctg cacctgactg gtacttgcat cactccagtc 60 ctccagcaac caatgagcca cggaacgata tttccactct acctgattat atgattcaca 120 aagagtttaa tgttgatgac catcatagta gtaattatga gacaagaaga gggccttttg 180 attttgatac aaaaagtgtt tggcaacgag atgctgagga caaagaaaac acagagaaaa 240 aaaaggtaaa gctcccagct ataaatgcta aatatccaag cagaacagca aatgtttgta 300 tgaacaagga attcagtggg aaaaataagc tttcctttcc accaatgcct gctcagagaa 360 aaggtgaacc agtgaacttt agcaaattaa ttagcaatgg ttatgggaca gactggcttc 420 agcaacatag tggatataga aaaaagattc aagatacgtc agaagatagt gagcaacccc 480 aagattcaga gccatcacag tctgaatcac cgcctgcaag caatgaattg aagccacaat 540 ttcaggagtg taaacagtaa ttaaaaaggc tattggggaa agtaaggtat tatataacct 600 atgataaagt ccactattta catctttgct tgattacttt tatcttatta ga 652 <210> 28 <211> 838 <212> DNA <213> Gallus gallus <400> 28 caccacctgc agggcagccc tgctggccag gggctgccct ctctgcctgc agtgccctgg 60 ggtttgtatt gtggtgctgc ctatccctct ctgctggtac ccatccagac tacaacctcg 120 taagctgtga tgtcatagtt atcatcaaag tattgctgga gtgagtttac agaagaaaag 180 atatccagca gaataacaag aaaaattatg actgaaggga ccagattctg caaaaactgc 240 aaacgagatg tgtctactgc caatttctcc ctccatgagg cccactgctt gcggtttctc 300 actctctgtt cagaatgtga tgaaccagtt gccagaaagg atatgaaaga ccatcaaaca 360 gaagcacaca agcaggtcag atgtaatctt tgttgccgaa gtatgcagca atatcagctg 420 gagcatcaca agaccaagga atgtcacaaa cgagcaatga aatgcaaaat ctgtgaattg 480 gaaatgccct tcaacaagct gcaggaacac ctgaacacct gtgccagtag aacagagcgg 540 tgttgggagt gtaacaaata tgttatgtac aaagaccaga aaaaacacaa agatatttgt 600 cagaacagtg gtctgtccta ttataaggat gtgaactttc aaaccaggaa tgcatatact 660 aatgcaaaat ttattgaccc cactggtgcc agtgacaata tttgtctgag gtgcaataag 720 tcattcccag acaaccagta ctcccaacat ctgaagaata actgcagcgc aacccataat 780 ctggactgaa gttcttgctg gccagtcaac ttcaaaaccc gtcagtgatt caccactg 838 <210> 29 <211> 1032 <212> DNA <213> Gallus gallus <400> 29 cccccttccc accagggcac gagcggggcc tggtggcaca acgcatgcca acagagggca 60 cgggatcccc tggccactgc gatgctgttg tcgagttgct gggggtagac ctgagattga 120 acggccccgg cttgtgagag gacatggagg agtttttgct tgccagcggc taccagctcg 180 gcaaaaccat tggggaggga acttactcca aagtgaagga ggccttttcc cataagcatc 240 agaagaaagt agcaattaag ataattgata agagtgcaag tccagaagag ttcattcaga 300 aattcctgcc ccgggagctc cagatcatcg agcgcttgga ccacaagaac atcatccacg 360 tgtacgaggt gctggagtct gcagacggga agatctacct cgtgatggag ctggccgagg 420 acggggacat ctttgactac gtgacacacc aaggcccgct gcctgagcac cgcgccagga 480 cgctcttccg ccagctggtc gaggccatcc agtactgcca cggctgcggg gtggctcacc 540 gcgacctcaa gtgcgagaac gcgctgctgc agggccgcac cgtgaagctg acggacttca 600 gcttcgccaa gcagctccct gtgggcggcc gggagctgag ccgcaccttc tgcggcagcg 660 tggcctacgc ggcacccgaa gtgctgcagg gagtgcccca cgacagccgc aagggcgaca 720 tctggagtct gggcgtcatc ctctacgtcc tgctgtgcgc ccagctgccc ttcgacgaca 780 ccaacatccc acagatgctg tgccagcagc agaaaggcgt ctccctgcct gggcaccttg 840 ggggtctcca gaacgtgcca ggacctcctg aaaaggctcc tggaaccaga catggtcctg 900 cggccctccg tggagcggct cagcaggcac ccctggctgg ccagaccctg agaagcgctc 960 accccgcccc ctcccccggg gagggatcgc ttctaggatg ccaataaatt tggatgtcag 1020 tgtttcccac gt 1032 <210> 30 <211> 840 <212> DNA <213> Gallus gallus <400> 30 catctcctcc cctcacctgt catcttccat cttttagccc tgaccccaat catgagtgtg 60 gggcaggggt tgtgtgagtc agagaaggtg tccctcattt gcggactgat gcgccaatcc 120 cctccagggg agttcaggca ggttgtccaa gatctctgtg atctgctcca agatgatgag 180 ctggtgaagc agcaggctgc ccgcgctggg gcccgtcaca ataagaacaa cttcacccct 240 gtccttgtaa atgggaacac tgtactactg actcagtaca atgacttagg aggaaaccgc 300 ttcttttacc cccaagacaa attctccttt gagtttgacc atttgagtgg ggtaaccagc 360 aaaacccatt tgcatagagt aatgctagat gagggggagc tgtggagagg agccctccac 420 aagggcttga atgcctatgt gaactaccac tttcctgtag ggaactgctg tgtgttcaaa 480 aaaagcctgg gaaagaggca gatgttagtg gcctgcattg aggctcatca gtaccagccc 540 tcaaaacact ggaacagcct ttggaagtct gactggactt tttctctgac tccagttatg 600 acccgagtta cagggatatt tcttctccag ttacactact tcagaaatgc taacctccat 660 gtaaccatca gcaagtctgt aagcgagtct ctgcatgtga tagaccgaaa tcagtttgtc 720 acagatttcg tgaagttcgt gaaaactgaa gacaacaaga tccataatgc cattctggaa 780 aacatccagg ccttgtcaga gcatacgtgg aggaaaaatc tgaggaggag acttcctatt 840                                                                          840 <210> 31 <211> 890 <212> DNA <213> Gallus gallus <400> 31 atctatagca gtctcagagc aaatgcatgg cagtggctgc tcctcgtttc ttaaaccaaa 60 accaagggca aaatccacag ctcaccactg atgtctgtgc tgtcaacagc ctctctgtgt 120 actggccctt aggagaggtc tgaaggcttc cactcaggga aggagagaat agaactcaag 180 ggcgacgcgc tgcttgatgc tggggaatct atgaagcggt tggctgaagt gaaggactcg 240 ctggatattg aagtcaaaca aaattttatt gatcccctcc agaacctgtg tgacaaagac 300 ctgaaagaga tccagcacca tctcaagaag ctggaaggca gacgcttgga ctttgattac 360 aagaagaagc gacagggaaa aatccccgat gaggaactcc gacaggccat ggagaaattt 420 gaagagtcca aggaagtagc agagaccagt atgcacaacc tcctagaaac tgatatcgag 480 caggtgagcc agctgtcggc cctggtggat gctcagctgg actaccacag gcaggcagtg 540 cagatcctgg atgaacttgc agaaaaactc aaacgcagaa tgagggaggc atcctcacgt 600 cccaggcggg aatacaagcc caaacccagg gagacgtatg actttggaga gagtgaccaa 660 tctaacgggg gcttctcttg tactcctacc cctaaagtct cagcttcctc ctctttccga 720 tccgacaagc cgttccggac ctccgtcagg agtatccctc acctggacca gccctgctgc 780 aaggcactct atgacttcga acccgagaac gatggtgagc tgggcttcaa ggaagtgaca 840 tcatcaccct gaccaaccag atcgatgaaa actggtccga ggcatgatca 890 <210> 32 <211> 749 <212> DNA <213> Gallus gallus <400> 32 cctggcaatg cctctaaagg ggctgtctga agacctagag gggtactagg ggtttctctg 60 ggacctgtgg gctgctctgt ggtcttccag gatgtttggc ttgcaggtgg actgtggctt 120 gctgctggag cccctgggct tcatcaaggt cctcgagtgg attttttcca tctttgtttt 180 ttcaacatgt ggaggctttc atggtgaaac taccctttta gttacctgca agggtgtggt 240 gaacaaaaca gttacagctg tgtttgctta tccattcagg ttgaatactg ttcgatttag 300 tgcaccagat tctaatcact gtggtggtac ttggacagac atctatcttg taggcaactt 360 ctcctcttct gcgcagttct ttgttacgct tgcagcactg gctttcctct actgcattgc 420 tgccctggta gtgtatgttg gatataaaca tgtgtatcag cacaatagca agtctccact 480 aactgacttg gttgcaacta ttataacgac ttttctgtgg ctggtcagta cttctgcttg 540 ggcaaaggca cttgctgata tcaaaatatc tacgggggct ggcattattc cactaattga 600 ctcttgcaaa gctccagaaa caacttgtca ttttggctct gtgaccagca tgagatctct 660 gaatgtgtcc gtggtatttg gtttacttaa tatggttttg tgggggagga aatatctggt 720 tttggtacaa gacaccacct tgcacagcc 749 <210> 33 <211> 788 <212> DNA <213> Gallus gallus <400> 33 tcggatgctg cctgggctgg aagcagctac acatagattt gggcctgaag tcctgtttct 60 ggtcatttaa tatgccagtt ggagtattaa catatgaaag gaacacccac cttgccccct 120 tgccttccca cacccataca acatcacagg acacactttt ccatcatgca aaattctcaa 180 agttctctcc agtcctaaaa tgaggcatct cagctggaca gaagataaca agattgtctt 240 acttcggtgg acacttatca tttgagggaa atgatttcag gtgtgctcct tgggaaacat 300 tggcctggtt aaagcctctc cctccttgaa ttttttataa gatattttcc agtcagtacg 360 tctcccctgt acagcataac ccagccactg cctctttctc cagacaactg catttatctg 420 cttccctttt tctatggtct ccctctcctc tcatcagcac catttcctca cagcacttac 480 acatatgcac aagcgaaagc gcatcaaagc taaagcatgt gttttaagtg ccatgacttg 540 ctgtgtgagg atggagttga agtgcaggac agactgcgat ctccaagaat gtctctctgt 600 actaacttgt gagctcatat ggttccagac agtcttcaga tgcacctgct gagatcccaa 660 gaagcactgg ggactttttc aacctccagg cattactcta aagacttaat gaactgaaaa 720 cattttatcc actaatgaca agccactggt ttttgatccc ttttcatcaa tacaatacat 780 ttataaag 788 <210> 34 <211> 1047 <212> DNA <213> Gallus gallus <400> 34 aagggctgag gggagggctg aaggcaaacc acatgatgaa aaaagaaaag aaaaaataag 60 aacaataaat atatgaaata aaagaaaaaa gaaaagaaaa ataagaaaaa gagaaaaaat 120 gaaaagaaaa aaatatgaac aaaaaagtga aaggggagaa aagaaaaaaa aatgaacaaa 180 aaagagaaaa aagagaaaag aaaaaaaaat gaacaaaaaa gagaaaaaag agaaaagaaa 240 aaaaaatgaa caaaaaagag aaaaaagaga aaagaaaaaa atgaacaaaa aagagaaaaa 300 agagaaaagc taaaaaaaga aaaaagataa aaaaagaaaa aagagaaaat agaaataaaa 360 aagaggacag aaaaatatga aaaaaaagaa aaaagaaaaa aagaaaagaa aaaaataata 420 gatgaaaaaa gagaaaacga aaggaaaaat ggttaatggc aagagaagaa aggtaaagag 480 aaaagaagaa aacagccaag tcaaaaagta aagaataact cgaaggaagg agtggatggc 540 aagaaataca gggaaagcaa tgcatagcac agcaaagcaa agcaaggcaa aatagcagcc 600 aaagccacga gaatcaagaa gtcagtgaag agcaggaaag gaatggagga atctggccag 660 agctgtgccc ctgcttgcca caggagctgc gcgggaggag aagaatgtgg gccaaaagga 720 atggaggaat ctggccagag ctgtgcccct gcttgccaca ggagctgcgc gggaggagaa 780 gaatgtgggc caaaaggtaa gaggagctac cgggcatggc cttgctcagc aaggggagga 840 aaagcctgaa gggcaccgag aaggggacag agaagggcag gcagtaggcg tgaagctgtg 900 agcagagtaa agagcaactt ggcagagcag caagcagagt aggcaagtgc ttgaggtttt 960 gccgtgagta ccagcaaaag cccatagcat caagaagcag gaagtgagga gaaggtaagt 1020 gaagggcagg caaggtaatg ggaggca 1047 <210> 35 <211> 1259 <212> DNA <213> Gallus gallus <400> 35 ctttggtgag ccctggcccc aatgcagctg ctggcatcag acctgctgat tgcagtggcc 60 cttttcacac agccttgggg acacaggcag ccccacatgg ccatggcaat ggatgacaga 120 cctgcaaccc ggtgagcagg gctgtgcagg ggagggcagc acagggaagc agggcaggag 180 gctgctcctt tcttccctcc cctctgcctt gcagatggac agcccaacac aaatggcacc 240 aggtgaccat aagtccagca gctcagccct ccaaactgct gttcatgccc tacaaaaaag 300 ataagtcagc cccatactgc agccagtcga gctcctacag gaccatggaa gctccccaag 360 gtccccctcg tcttacccca aacctctcac tctgctatgc tgagtcctga ccccactgca 420 ggtgctggca ccacaccacc tgattgcagt ggccgtgtca acgtggcgtt gctgacacag 480 tgagccccaa atggccaagg aaaccatttc cagaccagca acccggtgag cagggctgtg 540 caggagagag cagcacaggg aagcagggca ggaggctgct cctttcttcc ttcctctctg 600 ccttgcagac aggcatacgt accaagatgg aaccagagca ggacacacac ctgtcttcag 660 ctctccatct gctctccatc ccacacagga cagattagac agccacaaaa agtgcccttt 720 ctagggattc cagcaggtcc ccaagatccc cctgcccgca ccccaaagtt tgatacagct 780 tttctgagcc gtggctccac tgcaggtgct ggcatcagac ctgctgattg cagtggcccc 840 taccgcacag ccttggggac acaggcagcc ccacatggcc atggaaatgg ctggcagacc 900 tgcaacacgg tgagcagtgc tgtgcagggg ggggcagcac agggaagcag ggcaggaggc 960 tgctcctttc ttccttcctc tctgccttgc agatgggcat ccacaccaac acggaaccag 1020 agcaggacac acgcctgtcc gctgctctcc atggcacaca ggacagataa gtcagcccca 1080 gatagtaccc attctagaga ccctggaagc accccaagga ctccttcccc taccccaatg 1140 ttcagtgctg ctttcctgag ccctggcccc actgcaggag ctgtcatcag acctgctgat 1200 tgcagtggcc cttttcacac agccttgggg gacgcaggca gccccacatg gccatggca 1259 <210> 36 <211> 1113 <212> DNA <213> Gallus gallus <400> 36 gcagcgcgcg gccggcggcg gggcgcatgg ccgcccccgg gcaacggccg ccgccatggg 60 cggggcgggc ctcctcctgc tcggcctcct catcgtcctg ctgcaggcgg ccccggcagt 120 tcagtgctta agaagtgcag agtcaagaag caatagctta aaaatagtgg gatccatctt 180 atttccagtg aaagtttatg tcaagctgga tcacagcagt ccacatattc tatgtgttac 240 aaatcgtctg cgaaactcag aattgattga tccagtattc cggtggaatg gaccaagtgg 300 ttatctttct tcagcaaata gcagtgtgca aatatcacca acgggaactt taatattggg 360 acacttcaga tctgacctga gtggagttta cacttgttct cttgtttata agcttatcgc 420 tgcacaacca gataaaagac tcacaataaa ataccatatt tatgcttatt ctgatcctca 480 gtattactat gaattgacag tccagtacca tgcagccccc tgcaatagtt ttcacaatac 540 ttcttttggg agggcattgc ttcagatact aagcaagctt gttgctgacc tttcatgtga 600 ggtttcatta attaagtcag aatgccacca tgtaaaaatg cagagaggag gcctccagag 660 cgaaatgttc tttaaatttt cagttacttg cttagacaca gaaaacaaca aactatgtcg 720 gcaaagtgct tgtgataaac ctcatagatt acacaaggca aaggacctca tagagagatt 780 ttttaagcga gaggtggaaa ttaggaagaa aagtactgaa ccattgcctg aaatatacta 840 cattgaaggt actctgcaaa tggtgtgggt gtgatcgttg ctatccagga tatggaatta 900 atgctgtaag gcatccagac tgcccagagt gttgtgttat ctgcagccca agatcttaca 960 atcctagtaa cggaattcac tgtcttcagt gtgacaccag tttgatatac ggagcaacga 1020 catgctaact gatggtagag ggactggcaa agctgcaata gctctcatgc attttaacct 1080 ggtaatatta ataaatattt aaatatttac ctg 1113 <210> 37 <211> 705 <212> DNA <213> Gallus gallus <400> 37 caaagaacac agcgtgcaac tggaaggcat gtctcaaagc cttcacgcct cccgaggatt 60 tttgtctttt acactgacgc agcctcgcta cagctccatg ggcgtcacac aaggcctgca 120 ggacctcttg ctccgcaaac tctgtctgca ggcattgctt catgaggttc accaccggcc 180 cttctcaaga actaactcgt tgccggttgg tccagatgtc tgtatgctcc ttcccatggc 240 ccaggccaag cttgctgagc tgctcctcct ttgtgcagga cgacaacggt gcccatggca 300 tgtggtggct atgcacagca tgccttctac ttgcttttcc tctctccgtc ttccatcacc 360 aaacagcacc tccaatctct cccggcttac acaagctcaa cctacacgga aaagttattc 420 ctctgcgtct gcacacttgg cacgcatttt cagctgcagt agtcagccca gttctcagca 480 cagctttacc atccacagaa agccagttca cggccgccat ctttttctct cttcttaaac 540 actctactcc ggcggagctg tcctcagcac tcgctcccgt tgtgccgcgg cttccggctc 600 ggtgctgccc ggacagctcc ggctgacgcc gcgctctcct gcggggcggc ccgggctgcc 660 ggagcggcgc ttcgctgacg acagccgcgc ctgacactgc cggct 705 <210> 38 <211> 588 <212> DNA <213> Gallus gallus <400> 38 gggggggggg gggggggggg ggtggggggg gggggtgggg tgcggggggg gggttgtggg 60 gggggggggg ggggggggtt tggggggggg gggggggggt gggcgtaggg ttgtagtgct 120 aatgcaccag ctcagggctc ccatgttgaa tgggacacat acggaagcaa cagagatcac 180 ggtttggctc accggtttag atggattcgc tttgtgcagg ctatggctgg cacagccagt 240 ggttgtggct ccaatgctca gctgcatgaa gcgtctgcac agctcaggga tgtcgtcctg 300 cggcgtgccc gaggtcggag cggtgccatc agtgccatga gcagtctctt gcagctggaa 360 ggctggcaca ggctggggga tggctctctg tgcccttggc tgggctgaga agtcttctcc 420 cctccaggca aacatcgccc aaccgacggc tttcccaaag cctctgtttc gagatgccgc 480 cctttccttg tgccctgcag gctgtctgcc actctcggca gcagcaaagg ccggctgttg 540 gtgaggctgt gtccccttgc agagcctggc aagagcagga gcactgcg 588 <210> 39 <211> 775 <212> DNA <213> Gallus gallus <400> 39 ttggccctcc ctgctccttt ctgggtctca tccttcccac acttcccact ggaaacagga 60 ttttccttgg gaaagcagca gccccattgc cttaccagag aagaacgaca gtgccctgca 120 gcagggtggt cctccccagg aaggcttctt cctctcatcc cgaggtctct gcggagtgaa 180 cagtgaagac agacaggaaa aatgaagggc tcaaagcagc tgagagggtc cagttcgatg 240 cacatgccct cagacttccc tgtgacagaa aggcaggctc agttggagtt ctacaggctg 300 cagaaacagc tccagatagc tgtggagaag agaaaatctt ctggtgccag catgaggcaa 360 caaatgcaaa ctcaggaaaa agaaatccag tcactgaagc aagaacacaa agagatgtca 420 ttattgctga gcaagatcag gtccctgaga aatatgattc acgatgacag aatctgcatg 480 gagctgaaat gccttctgca gaccaaggat cagtacgatt gtctgattag agacagaaaa 540 gccctggtgt ctgagctgga caatgagatc ctagagctgc aaaagaaggt tgagagggaa 600 aacgtgataa caacgaaagt gaagcaagca aactgcagca aatggctgca agaccagact 660 cagacactgg agctgcatct gaacaacgtc actgtgcgct tcgataccat cctgaccacc 720 aataataagc tccaagaaag agatcgaaaa ccttcgaatt cagaaagcca ctttg 775 <210> 40 <211> 635 <212> DNA <213> Gallus gallus <400> 40 agccgcgggg ctgcaggggg aggcccggcc cggttctctg cggcggggaa gggattccaa 60 cagagggaaa acatgaacga ggtaaaggat actctaaaag tcatagaaga caactacaag 120 ctcttcctgc agcagcagtt cacgtttgtt ggagctctgc aacacacccg agagaacgca 180 cacgacatga tcagacccgt ggcgagcatc agccaggtac agtcctacat ggaccaccac 240 tgtaacaact taacggacag gcgtatcctc accatgttcc taaacatctg taatgacctg 300 agcaatctct gccacaagct ggaaactcta catcctggta acagcacaac caatgaaatt 360 ttggaaagat gcaagctgct ccttaaccac agcaatgatc tgagcaccat ccgagctaaa 420 tacccccacg atgttgtgaa tcacctgagc tgtgacgaag caaagaatca cttcggaggt 480 gtggtgagcc tcatccccat tgtcctagat ggtgtgaagg agtggctatc ccacactgag 540 aagctgccgc gacgcatgct gtgtaatgca aattagctcc tgttctttgc ttcctgctgg 600 aagacactcc tgaataaaca atttcccctt gcaaa 635 <210> 41 <211> 851 <212> DNA <213> Gallus gallus <400> 41 gaaggcacca agagggtgct gaagccccag cagaggggct ggggtgtgag ggaaagaaaa 60 aggccccact gggagctgga gcatgctgag gtgatggcct gccaaatgct agcaatgtca 120 tcaaagtgaa gaaaaaggag ctggcaacgc ggagccacca tggaggagaa cagatggttt 180 ttgaacatca ttcaggatgg cttcatgaat gggaagattg attttgatag caccgttaaa 240 ctacttgaaa aactgcatat gccttttaat ctcgcccatg tgaaacatgt tttcaagaaa 300 acagttgaca agcggaagat acatactatt aacatagaag acttcagagc aatttatcgg 360 gctattgtac acagaaatga atttcatgaa attttctgcg cttactctga aaaccgcaaa 420 aatcttgctg acactgagct cactgcattt ctcaaaaaag agcaatttaa aactgaaggt 480 gctgaaacaa ctgccttaga agttatcttg aagtatgaac ccattgatga agttaggaaa 540 agaagacaat tgtcatttga aggatttata aggtacatga gttcagaaga ttgtacaata 600 ttcaaaaaag agcaccggac cgtctaccaa gatatgaacc accctttatg tgactatttt 660 atttcatctt ctcacaatac ctacttggta tctgaccagc taataggacc cagcgattta 720 aatggatata tcagtgctct gttaaaagga tggtcgttgt ctggaaattg actgctggga 780 tggctcaaca acgatcctgg tgtgtatcat ggtcacacct tacaaagcaa aatacatttt 840 gttctgtaat c 851 <210> 42 <211> 642 <212> DNA <213> Gallus gallus <400> 42 cctaagtttc tggctacttc ttgtttaagt tcagagcaaa acaactttgt gtgtcattca 60 aaatacttat tgagaaaagc caacaaaatt tgttccagag actccaaaac cattattgtg 120 ctttcactga ttacttttta cctgctgaga tcaagaacca tccaagagaa actggtttat 180 ttcatcttca gttaattttg gaatacggct ccgacccgat gaccacagaa gtgcctaaag 240 atcaaagagc caaagctgca gagagcattg ggttgctcac atatacaggc tgcaaagtaa 300 gctgcagcac atgtcagtgc tgtggtcact cagtgaaaat gagacaaatg caagatgaag 360 atgatgcacg tcaactccag gcttaaagca ttctgacaac tgctttatgg agcattagag 420 cttgtgttgt agcaattaga aaacatcaaa aaatgaagag ttccaatggc tgcctccact 480 ggattggaat aactggccaa ggaattaggc aggagagctt tatagccttc tgtggctctg 540 agaaaatgta aactacccca agatgttgaa aataacacca cctgcaaaga acagttataa 600 actatgaata aattcaactc cactgcagaa agaggtctgc tt 642 <210> 43 <211> 521 <212> DNA <213> Gallus gallus <400> 43 aatgctctgg tgctgctggg tgcctggcag gtctgcccat cctgtgcaca atgtcaggca 60 cggtggcagc cggggcaccc gtggtggttt ttgacaatgg ctctgggcag tgcaaggctg 120 ggctgtcagg ggagcaggca ccccgttcgg ttattcctac cattgtgggc taccccaaat 180 tcacgtttgt catgatcgga atggaacaca gggactggta cgttggggag gaagcccagt 240 ccaaaagggg cattttgtct ttaacttacc caatggaaaa cggagtggtg acatcctggg 300 acaacatgga gaagatctgg agatacctgt acgaacacga gctcggagtg aagcccagcg 360 agggaccggt gctgctgacc gagacccccc tcaacccccc atcccaccga gagaagatgg 420 ctgagctgat gttcgagagc ttccaggtgc ctgcgctcta cgtggccctg caggggctgg 480 cagccctcca cgcctgcgcc cgcaccaccg gcatggtgct g 521 <210> 44 <211> 961 <212> DNA <213> Gallus gallus <400> 44 cagccagcag caacaggcac tgccagggca caggatgccc taagaaagca gaagcaggag 60 gctcccggga cgcctgtcct tcctgagtgt ttctcctatg cactgctgag cccgtgggct 120 tacagggcag ctcctggggt gacggggcag cagctgctcc aggaggacct gagctctctg 180 ggccgtgatg atggctgaag caatggccac ggtggacaat gaggacctgt cagactattt 240 cagcgtgcaa tacaggcaga atctgctgcc cttgctcaag aaactcaaac tgactgagga 300 ggactctcta tcgcctttta tccgcctcca ggagaagaag aaagaaaccc aagagatgca 360 aagggttctg gaggagaagg aagaggcctt caggctgagg atggagggca tcacctgccg 420 gtggaaggag ctccaggcca aggaggccga gctgaaggct cacatgaaaa aatctgagag 480 gaccctaaag gaaaatgata agatgcgaat ccaagctcta aagaaagctg gcaaagaaag 540 ggagatgaag atacagaagg agagtgagct cttgagagca aagaaggaac tggaagccct 600 gaaaattaag caccagaaac tcagtgacag agtgcagaac tactccatct ttggaacata 660 cctggaggat gtggtgaagg tatcacagtt tgaggagatc caggtaatca tttggcgcta 720 caaaatgtta gtgaggatgc gcaaggcctt gctgcaatcc tgaccgcggc ctcagggagg 780 caaagcagaa agccaacgac ctcaaccaat atcgttgaag ctaacagcct gcataataac 840 aacgacctgg atcagctcca actgctgttc tcaacaacct cacagggatg ttcctacctg 900 gatgtctcgc tgggctgaca tgcagaaaac agctgccaag acaacccggg agctggggac 960 c 961 <210> 45 <211> 878 <212> DNA <213> Gallus gallus <400> 45 cggcaacggg ggcttgggct gaggcgggcg tctgtgatta tacgtgttca gtagaaacct 60 aaaccagaaa ggacagctca tctgatgaac cattccagcc tcttctttca cggcttccta 120 aaacttcatg tgtgctagaa ctgcgctttg aggggacaga ggaccattca gtttaatgct 180 aattgtggtg cctttggtac atcagttgtt tgtcaccaag cagagtcatt ttgcagtact 240 tttttaagca taagaaaagt ccttcccaga caaattagag atggcgagta ctcttatctc 300 agccaagctc ccccctgatg tagatccaac aaaagctcct attgcatttg ggaggagagc 360 tcttcctaaa ctgagtagag agatacaatc tcctgagctg ctgactcagc aacgggcatt 420 gatggcactc tgtgatctgg tccacgaccc agaaaatgtc taccaggcag tccaaatagg 480 tttcttggag aacttgaaga ccttgctgct gcatcatgat aatactgtcc gacagaagac 540 aacagaaatt ctctacataa tggcaatgca taatgttggc aggcaagggt tgatacaaaa 600 tgcagtaata cctgtcctca ctgaactttt ggatgaccca gaagacatct gtcggaagaa 660 cacacatcaa gtccttgaca tgatggccaa gttacctgaa gaccactgta tatttaattg 720 acctgtggaa gtaaagaata ctgtactcat ataagagctg tgcatgtcag cctgaaatct 780 gaccacattt gaagaacaaa catcttctga ataatatgtt gttggactat tctgttgacg 840 ttctataact tgaaacatta agaaaacctc catattct 878 <210> 46 <211> 530 <212> DNA <213> Gallus gallus <400> 46 cttagccgga cgggatttca ccccctgcgc taattctgac cggtgccaac acagatgccg 60 gcagaaaatc aatgctattc tatgggctgc ataacctgca acgcaggaaa tgctttccgt 120 cgtggtttct gcagaccttt tgaagtctca gactgacgct cgccgattcc attattaaca 180 cacgggaaaa gcgcccaggc tcagacggag cgaaccgccc gcagcagctc actgagagcc 240 gccagccctg cagcccttct ggcgcagaac cgcttcgctg acccctcaca gagcttcccc 300 gtttttaccc tgcacagacc gagaagcaga ttgtttcaga agtcgcgtca agcagcgcac 360 ccacaagtaa aagccggcgt cgccctcagg gaagaacgcc acctgcacac cgagcccgcc 420 gagcagacgc ccgctccgac gcgctcagga gaagtttgtg cgctctgtgg gcagaggaca 480 cgtggaaggg ccgggctgca gcccgggaag gggcgaggcg cttccccccg 530 <210> 47 <211> 640 <212> DNA <213> Gallus gallus <400> 47 aagtctgcag gatccacatt actcgctctc gatacgccag aaaacatcta tacgatggag 60 gatctagaaa aatatgagga aaattataat tttattccag aagacgcgat cctggatgaa 120 gcaacagaaa aagacaaaaa agcagagaag gaacctgacg acttagatgc tggcagtaat 180 tcaccttcca cagagctaga ttcaattttt gggaagttgc caccaaagtc actgatcaag 240 accaacctcc tcttcccaaa acctccacgt cccatgacat cgtacaagac atcgggaaga 300 cgtcattccc tttctactaa caaacagatc gttgtggctg gcgacaaaag acaccaggat 360 gtgacagtaa aagacctgaa aactccccac aagagccacc cagcacagaa acgttcactg 420 agtcattgga acgctccttc ttccaaaccc agctcaagag ctgctcttaa caagcgcaaa 480 aatcatttgt gataacgcac aggctgagca acaacagcca ggaggagaac attaactgta 540 ttgcgctctg tttcactgct gcagactccc agaaacacca cctgctggga tactctgatg 600 agaattctgc cgaaatgtct gaataaagga aataaccctc 640 <210> 48 <211> 877 <212> DNA <213> Gallus gallus <400> 48 ctcgaaccct ggtccaggct gtggggagaa aagcaagagg agaaagaggg caccgggtgt 60 tggtgttccc ccggactgcc acgtggatcg gttcattcag tggcatcaag gctggacctg 120 cttgcagcgg ctttggacac ctcgcccacg ggatggatgc attgctgtac aactccccag 180 tggccgggag agcttctcca gatgctcatt gccaacgggg cctcccccag attgtggcga 240 tcctgggatg ctgtgtctga ttacatcctg gaacagatga agctggacaa gggagtcctg 300 gtcccgggac tcgggctctt tgctgcggtc cgagagcaaa gaaaccgcca ctgcaaagaa 360 gtggcggtgt tggtccgaag acctgtcttc cagctggaca tcggggtgct gtggctgcag 420 gacctccagt cgcccaccga catcatcccc gacgatgtca agattgagcg gctgaactac 480 cggcagctct cgcgagccac cggcatctcg ctgcacgtgg tgcagccctg cgtgcaagag 540 accgtcctct tgtactgtca cctcctgagg aacaaggcac acgtctcatt cgccttcaag 600 aacatcggcg ttatgacgta cgagaacgac ttcctctgca tgaggttctt gtccagctgc 660 attacaacgc tggagagcaa tgccagcctg actgcactgc tccacagtag aatttggccg 720 gcccattctg ctgacttcag gccagagacc accactcgtg ggatccaggt gttcccgagg 780 tttcagctga ctgtgaaaaa gagcagagca gaggccgccg cataaaggaa agctgcagtg 840 gagcggaaca tgagtagggg ggggcactgc cggcagg 877 <210> 49 <211> 721 <212> DNA <213> Gallus gallus <400> 49 ctgcctcagc acccagccca ggacctgctc tccagaggca catggcccct ccttgctcac 60 ggtacccaaa gcttatgaag aagatcctgt aagtgagaga agatggggtc tggctgcctg 120 aaagtcacca agtacttcct gttcctcttc aatctcctgt tccttctgaa aggagagttg 180 tcacgcatag ttgaaaaact gattggagat tatgaccctg tgaatggtga acataacaac 240 ttgcaagatg cgtgggacta tgtgcagaaa cagcttacct gctgtggctg gaatggagca 300 gaggagtggg agaaaaatcc cattcttatc aacaaaagca tgactgcgta tccctgctcc 360 tgctccaata gttccaagga tgctgaggaa aatacaggct tctgtacact ggatgtcgtg 420 gtgaatgaaa ctgcaacgca tgccaactgg cctgtgcaca ggcacggttg tgtggatggg 480 gtacaggatt ggttgaagga caaccttggt atcattcttg gagtctgcac aggcgttgct 540 gttgtagagc tgctggggat gatactgtcc atttcactct gcaagaacat acacagtgaa 600 gactacacca aagtgcccaa gtcttgagtt tgttgactcc agaactacgg caccacagag 660 aaaggagcaa acagaacatt ttctgcctcg tacccagact gtccaaccat tgaccattac 720 t 721 <210> 50 <211> 780 <212> DNA <213> Gallus gallus <400> 50 ccgggcgagg ggcggtggct gtggcagtgg gatgggatgg gatgttatgt tcgtgcatcg 60 ggctgcggag agcttccatc aggaagcgag atctcttcgt agcttgctgc ttttccggag 120 atggctatgt gtactgtgct tttccatcaa gtgatggagg cctgaagctg cccatcgtgg 180 agatgcattc tgaaggaact ggaatcactg gggaaagcac tgtatggggc aaaattgatt 240 gcccaaagtt ttcaagttcg aagctgttct caccacaagg atcctgtggg tggctcggcc 300 tgtttacttt ccatggttga agaaggcaac cctcatcact tctttgtggc tactcaggac 360 caggatttag caaacaaagt gaaaaagaag gctggcattc ctctcctctt tattattcag 420 aacactatgg tgctagacaa accttctcct aagtctttgg catttgttca aacgttgcag 480 acgagtcagc ttgttccaga gcaccagaaa caaagcatcg tacaacttaa agaaaaagaa 540 ggactagcaa agcaagaagg tgaaaagaga agaaaacgta aaagggcagg tggccccaat 600 cctctcagct gtctgaagaa gaagaagaaa acacaggaga gccagaaacc tgctgaaaag 660 aagaaaagaa gaagaaagcg aattacggct aaaacagaag ccgtgcagtc agtgcagaag 720 aatgagaaag aataacccat ctttgtgagc aatgcaggac attgtttgat ctctgcaaag 780                                                                          780 <210> 51 <211> 823 <212> DNA <213> Gallus gallus <400> 51 ataatggctc aggtaaaacc acgcttatgg ctaaactaca gggagcagaa catggccaaa 60 aaggaagagg actggaatac ttatatctca atgttcatga tgaagacaga gatgatcata 120 ctcgctgtaa tgtttggatt ctggatggag acatatacaa taaagggctg ttgaagtttg 180 ccgtttctgc agaatccctg cagcacacca ttgtaatctt tgttgtggat atgtctagac 240 cttggactgt tatggagtct ttagagaagt gggccggtgt cttgcgagag catattgata 300 agctgaaaat tcctccagaa gaaatgagag gcatgcaaca gaagattata aaggagtttc 360 aagagtatgt agaacccgaa gaagcctacc aaggatcatc ccagagaagg ggcccttctt 420 cgggccaaga gaatgcaagt gtttctttgt cacttggaga aaatgtgctg acgcacaacc 480 ttggtattcc tgtgctggta gtttgtacca aatgtgatac agtgagcgta ctggagaaga 540 agcgtggtta cagagaggag cactttgact tcattcagtc ccacattcgt agattctgct 600 tacagtatgg agctgccttg atttatacct cagttaaggg ggaaaaaaac cttgatctgt 660 tgtataagta ccttgtacat aaaatgtacg gttttcagtt gaccactcct gccttagtgg 720 tagaaaagga tgcggttttt atacctgctg gttgggacaa tgaaaagaaa attgccattt 780 tacatgaaaa cttcacaaga gtgaaaccgg gctatgcgta tga 823 <210> 52 <211> 318 <212> DNA <213> Gallus gallus <400> 52 agctgtctgg ccccaacggc cactctgtaa cgggcagcag cagcagcagc agcagcaaca 60 gggatcctct gctgtgcggt gctggctgcc gtagggggtg aggatgtcca agcaggactt 120 gaaggcaccc tttaccgtgt cgtgccacct gtgccacaga gagctgaaca ggatttcctc 180 tgacacaaaa gtcttctgca gtttgtgcaa aaccaggtta gagacagagc aaaattctga 240 ggagaatgtg aggattttga aggagtatag agatgcgatg gaggtagagt ccaccacata 300 cacaaacttc atttcctg 318 <210> 53 <211> 450 <212> DNA <213> Gallus gallus <400> 53 cgcgaggact cctcgagcgc cgcacctgca ccgcaacgca gcggctcgcg gaaccttcgg 60 gcacctgcgg atccgcgccg cgctctgtga gaaagcggag aacaccgcag tcatcaaagc 120 ggtcgctgag gtttaaacat caacacgacg gaaattcgag ctcctgtgaa gaggtcacgt 180 gaaattcgga ctgcagttat gaggcgctac gacgaagcgc ggctggcagt gggagcacag 240 ctgtgctgcg aggacaaact tagcgagggg tcacaggtgc ttaaagaagg agtttattga 300 caataccatc acaaagaaac gccctaaaag aaaattccag agacctctaa acttagaaac 360 tttaaaattt tgttctcatt ggcaaaatac accccccgag ggaaggaaat aaagagagaa 420 aagatgtccc ctgcatcctt ttaactaatt 450 <210> 54 <211> 573 <212> DNA <213> Gallus gallus <400> 54 aacctgctct acaacaccac atctgcctgc ccgctgcctg tcctacgctg gctcttccag 60 ctgacgagca tatggccaga caggaccaac gccttccgga cgctctggga gatgtggatg 120 agaagcgatg atgagccctg gtgccccacg ctgcaggaca ttggccaggc ctttgcctac 180 atgggtgctg acctggaggc cctgcgctgc cgccggctgc tgccccccga gctgtcccct 240 atggacacga ggctggatcc atcgctctgt caggagcagg gaaacctcag tgctgccgac 300 acgctggccc tggtgacaca gctgggggac atctgcaagc tgctgacggt gtgtgtggtc 360 gcctgcccgt gccgctaccc cgaccgtgcc cgccgggagc tggtgacact gctctgcttg 420 ctcagcctcg accacgcgct gtgctgccaa ccgctgcccg acctgtggac ctcctgcact 480 gcctgctgga gggcatcgtg gcctggaagg agcagctgcc cgcgctctgc ctgtcgttgt 540 gccagctttt ccagcaccac cacaacctgc ttg 573 <210> 55 <211> 571 <212> DNA <213> Gallus gallus <400> 55 ctaatcctca cagctgaaga ggtctccctc cagatccagg caggctcatt gttctcagtc 60 ttcctgaact gacaaatctc ccagggacat agtaggagag cttgctggaa taactaacaa 120 aggttcatct ctttcatatt tcctgtctga gaaaggattc tcagagaagt ccagcacaaa 180 aataacgggg aatgttttaa ggaggaccaa caggctacag actttctggg aagtggaagg 240 tgaagccact ggaagactgt aggatgactg gactggagga ctcttttgtg caagtagatg 300 gcacccctaa agaaaggcct tacttctttt agttctggta ctgggttgaa cgttaaatgc 360 attggcactg caggatgatt accaaagaat ttgagaagca aaatgaatgg cagttcttcc 420 tctccctaat taaaaaagat agttacagca atttattgtc aggattaatg cagcattaat 480 caagcagatg tgatctatta aacagctctg gttttgccaa aagaaattaa ttcagcacaa 540 ctggatttta taaaatcttc attgcatcta a 571 <210> 56 <211> 590 <212> DNA <213> Gallus gallus <400> 56 agagagctgc cggtgtcctc caggaacaag cagaggggat ggtcgtgggt gaggtttgta 60 caagaccaga agtgcttggg accaggaccc cctgggaagg ggcagagtgg atggacagcc 120 aagggcagga atagctgctg gtgcccaacc ctcatctctc actggtgtga ctgcggggca 180 gctctctgcg tggcaaagaa aaccagagtt gtgggtgaag tctccgcgag cctgtacgga 240 gcatttctat gtcagtagca gaaaagaaaa catgtaggaa ccaagtgttc aaatgttggt 300 ggaaaacagc tatatttagc cgctctaaaa attgcccagt acaaggcata gaggaaatgt 360 ctgtggcaac atacactcct atggtttcac ctgttgcggt gcttctaaag cctagaaatg 420 tcagtaaaat aagtagcaaa gcagctaaag cttccccaga agaagtgcca gcaaactaag 480 ctcacttgtt gaagcagccg ttgctgaact atgaattgac cttgagctgt gttgatttac 540 tgtaattcaa accatctgca ctctgaaaca attaaagcct tcagatctct 590 <210> 57 <211> 673 <212> DNA <213> Gallus gallus <400> 57 acacgggtaa cggcacggag cagtgtgagg actgcaaccc cgcttaacag tgtgccccgt 60 ggatgtgggg ctgccccatg gctgtggccg ctgcagtgag ggctcctccg tggggctgcg 120 gtgtgtgcct gcctgcctgc catcccaggg tgggccggga gaggagagca gctgagctgg 180 catggtgcct gcgcctgggg agctatttgg agatgcgttt gatgtctccg ccgcaagggt 240 gatggatgct ggggcgaacg tcgtgatggc acagccaacg ctctgtgctg ggctttgaag 300 tctcccgagc ggagcatccc agctcgcaga tttgggaaga catggactac ggggcaggag 360 atgctacttg ccaaggcggc tacgtgagga gcagcctcag agctcaacat cccagctgca 420 ccctgctcca ggaggacgca cgagttcccc gtcagctgtc ccttcctccg catctctgtc 480 actgtgtccc cggtgtgcag agcccggtga gatgccgcca cgcaccgtgg gctcctgaag 540 ccatggagca gcacagaccc aaggaggagg acgagcaggc tgaggtgatg ctgcccgttg 600 cttgcagtgg gtgctgcagc ttgcctggag gaagccccca caataactca gtgcgtaaac 660 gtgggaggat aca 673 <210> 58 <211> 338 <212> DNA <213> Gallus gallus <400> 58 cagccccggc ggtgctggga ggagtgggag tggcccgggt tgccccacat ctcacccact 60 cgagagcagg gccgtaggca catacagccc agtgagggga gttcctgggg ggcacgctgc 120 gaggctgagg tgccggcatg aggattgaga aggtagcaaa tgctgcggca ggggcacccg 180 gcaaggtgca gcgtgtgcgg gcagtctgca gccgcccctg gctctgccct gagcagctga 240 agctggcaaa gctgcaggtg gagaaggcta tcaaggagaa gaagatcttc atggtgcagg 300 gccgctaccc ggtgatccgc cgcctgctgc gcgcacgg 338 <210> 59 <211> 359 <212> DNA <213> Gallus gallus <400> 59 aacacaaacg tcctttatta caacatctag agagaagcat atccagtcct cctccaggca 60 tgttctgatt tctctctaag tgtgtttgtc tgttgatcac ccacagaaga tcctgctcat 120 gggtgaagct ctggatatgg gcacctgcaa agccaggaag aagaatggcg atccctgtgc 180 acagattgtg aacctgaacg actgtgagta ctgtcagtat cacatccaat cccagtacaa 240 gaagctcagc tcaaagaggg cagacttgca gtctaccttc tctggtggcc gcattcccaa 300 aaagcttggt aggaaaaatc ccagtttgaa ggagaggctg tgtcaggatg gatttcact 359 <210> 60 <211> 1351 <212> DNA <213> Gallus gallus <400> 60 cagccttcag ggagggacag caggagaatc cccaccctat ctaaaacctg acagagacca 60 ggtgacgtgg gacaccccaa catcagcagg tgctgcatgg cgaggtgctt tcctgctggg 120 gagaggagct ccctccctgc tttgcacacc ttggtggagc tgagttcgct ggcagaggag 180 gcaaggacgc cttcgctgcg atcgcacacc acccactcac acccccgggc tgccagcact 240 cccatcaaac gcctccagcc acacgcgtgg agacgctgac atgaggagcg aggagctgca 300 caggcagcgg aactgataac acccagtttc ctgtcgatct ctctcccacg tggattctct 360 gatgtctaat agcaaggctg agctgcagtg cagaactagc agtctcttcc tccccagacc 420 tttgagatag ctgctgcact gctgatctta actgaaacca gccagcaagt tcaaagcaga 480 gagaagactt gcaaataaac agtaagcttg cgcaagcctt catttcctca ggacaggaat 540 ctgcagacgt atctccacct ttcttcattt gccctagaaa cagctgcacg acctaagcag 600 cgtgaccaga aggctgtggt gcagcagctc aggtacctct gtagttcagc agcatccagc 660 aacgggaagc accctgcctc agcccatgtc agaaagcatt cagcagcctg cggtggcccc 720 ctcccatggc cacaagcact gtgaaccatg ctcacctccg tctcctttca tcctgccaca 780 ggcagtccca cagctcagct gtacatgggc ggggggagac gagccagcac atccacattc 840 atccctatcc tgccacctgg gcagcctcca agccctgcca ctccgtccct ccacggggca 900 caacgcttca ggactcctcg ctgctttgcc cttcacgtgt ttactctcca ttcatacagc 960 tctgccttaa agcagagacc ccacctcccc caggccgcag ctacccaagg gagcagggct 1020 acagcctgca tccctctgca agcatcccac ccagccctgc ccgccctgca cactgcgtac 1080 actgcgccaa ggggcagcct ccagacacct ccccgctgct ttgcctgctg ggcacaggcc 1140 ggaccgctca cactgccctt ctgctgctgg cggcagcacc tggcaaaaag gacacaggca 1200 gagccaggag ccgacggatg gcagcaagca attcagcaaa acgaagcgcc tcttgatagc 1260 gcgggtgaca gtcagaaagt tgggttgggt gctttgtgtt gtattttaag aagcctgcag 1320 ggacaccaac aacccgcgac aagcattttc a 1351 <210> 61 <211> 748 <212> DNA <213> Gallus gallus <400> 61 tgatgctctc cccacaggct ttctctttct ccagtgcttc ttaaaacatt caggagcacc 60 actgagctac caaggttttc aggagaccat caatcagact aaatcaaaca tgggagaacc 120 atgactttgg tttggaatct gtcaaggaaa ctcatgcaat cgatcaacac agaaacggaa 180 gaaaggtttg atgacagcaa gctttattct gacaaggagg agaatgtgaa ttattcagcc 240 atctccacag gatattttcc atattacaga tcaaatgagg agcagtttct ccagcctctc 300 ttatatggct taaatgcaga gggagagaac cctgaaagca actgcaaaga tgaaggagaa 360 ggtggatgtg tggagcttgg tggagatgct gagaagacca cccacccagc tctggggaca 420 gagaggaagc cgtgttcttc catggccaat gtagagccaa catcccagca gctcctagga 480 ggcagcacct tgggaagctg ccttccagcc ttcccccatc ccaccacacc accaggcctg 540 gaccagccct tttcttccag tggatacgtc ccttattaca gaacagaagc agagctctgc 600 gtcggcccag cagtgtgtga taggtttctc accaggacag aagttttgga gaatgaaaag 660 ggaagatggg taaaaaaaaa aatgcttgga attttggggc agctgctttg gtgtcctgag 720 aacaaagcag caagttttgt cagcaaaa 748 <210> 62 <211> 930 <212> DNA <213> Gallus gallus <400> 62 agaaggacag gttacaagag catctttcta atgctaaaca agaaaatcag gtactacatg 60 tgaaaataac gaagtaccag aatgagcttg atgagatgaa gttgaaagcc caggattcaa 120 acacagatat tgccaggctg aagggagtac tgaagtttaa agagagagag aactgtgagc 180 ttttggagaa ttaccacaaa gcctgtgaac aaggagaaag ttgggaagca aagtgccatc 240 aagcagaagc agattgtaac tctgttaggt tggcattaat cagcgtggaa tctgagaacc 300 gcaggttgaa agagaaagtg aagacccttg aaacagagat ggagcaactg cagagtgaat 360 gtgattcatc ccgttctgaa atagagctgc tgagaagaca actgggaaat gaaagagcat 420 ctctgaaaaa cctggaatct ttgcttgcat ccaatcgtga gagagaattt cagtcacaga 480 tagcagagca agaaaaagac tctgaaattc agcttctcaa ggaacaactt tctttagcag 540 aaaataaact cactgtgaag agtcgagatt tcactcagct caaaaataca acagctcacc 600 tagagtcaga actggatatc accagaagac agctggcaac tgagaggttt gaaagggaac 660 gtgctgtaca ggaacttcga tgccagaatc gtacagtcac atatcagtta agctctacat 720 taagaacatc atcccctgaa cgtgttcatc atctgcctcc tgactggtct ctggacagtt 780 ccctggaagg gaattcttca ttcaaggact tctaatatta tattcacaat ggcctgtgaa 840 gactctgcat gccatttgga gtgttcaagt ttaatttgca tttaattatc atttttccct 900 gcttaatttc attaaatcta tttcacaagt 930 <210> 63 <211> 469 <212> DNA <213> Gallus gallus <400> 63 cccagcagcc ggcgggcagc actgcgcctg tgcggtatcc tctggcacac ggcactctcg 60 ccccttccct ccgggcgcct ccatcccgcg gggggagcgg gttgtccagg cctctccact 120 ccgggtcctg cgaaccgccc tgcccgggct tccgccctcc agcccaggga aacaacagcg 180 gggctgccgc ctcctgcttg tacaaggccg ccgcagaccc tccgcactgc cgtagagccg 240 gcgcacctcc ctccccccca ttgcgggagc gttggttggc cctcccgggg cactctggga 300 actggagggg ctgcgttctc gtttccgctg cgcgtggggt ggcgtgctgt gcgtgagggg 360 atcggccgtg cgcgttgatg atcacccatc agtctgtcca gaccagaagg agaacctttt 420 ggaagatgcc aaagaagtgg aagtttggca gtggccaaag caagcaacg 469 <210> 64 <211> 375 <212> DNA <213> Gallus gallus <400> 64 cctgtccccc gggcggagga aggttacggc agcgtgactg cagaagaaga cctaccggaa 60 agatagcagg caagtcacac gtacgagttt cctatcagct gacaacacct ctggtacttg 120 aagaacgtgt tcacgtccca gtgagcaaaa gatgctgaca gaacaggaaa aatttggaga 180 catcttgggt acaattgtat gaagagaatg cagcatggga gagccctttg atgatctctg 240 agagtcctct ggacttgctc caacagatct gcatcccaac ctggcacaaa ggtacacttc 300 cacatctgta taactacagc tgaacagcga agttggattc ttcaaaatta gggctgagtt 360 ttctgcttta cttta 375 <210> 65 <211> 716 <212> DNA <213> Gallus gallus <400> 65 cttgccagca ggcagggcaa acactggctc attgcacaaa tggctgcacg tgcaaccttt 60 ggaaaaaaac gagacaggag atattgaaat ggagttgtct gcagcagaaa cagcctctgc 120 tgctgatcct gaggagctga ttgagacttc aggctccgct tctacagacc ctacaggaca 180 cgatcaagca ccatctggcc atgctgctgg aacatcaata caagaagaac aggactccca 240 gatgtttcca gaagaagagg aaaccaaagg tttacctagc agttcttctg aggaaagtgc 300 tagcctagcc agtgtcttct catgcctcag cttcccacaa aaactctggg tactggtaga 360 aagtgaccaa gtgaagagca ttcagtgggg tcacggagga aattgcattg taatccagga 420 agaaatgttc aaggtggaag ttctggcaag ggaagcacct gcgagaccct ttgagagtac 480 ctgcatgaag agctttgttc gccagctgaa cctgtatggc ttcaccaaag tgccacagca 540 cttggaaaga tctttccttc ctcaatttct ggccgaggga gaagcatttg ctgcccatag 600 gaagttactc ctctactcta gcccacactt cagaagagat tatccgcagc ttctgggaca 660 ctgcaaacga agagtagctc acaacagaag acccatggct gcccctgtcc ctgtcc 716 <210> 66 <211> 583 <212> DNA <213> Gallus gallus <400> 66 ccatcccgtg tgcccggagg gaggtgtctt taaaaatgga caaaaccctg atgtgaataa 60 gaaaataaac tgcaggaaat ccattgaaaa ggtactgcag ccaaacagat ggttgaattt 120 gatgccttga ggcctattct gcactgactc aactggttat gatgtgcagt tctactggaa 180 tgaagctgct gaccatcagg aggattttga agacaagtac caaggaagga gttgtgttcc 240 tgagcagaac tttgcacgag gactccatta tatgttttgg ttcgttatct cctgacataa 300 cagttctgtt ggcccaggat gtggtttatc ttatcacata gagatgatac cctgtcaaga 360 tatgctccag tttctgggaa caattgttct attgtgcctg gagaatgcct gggacaacat 420 gctattgccc actggcagag ccttacaggg tctgatggca caaagcaacc atgaataaat 480 ggacagtggg accaaaatgc aaaggaaaat tgatggaacc acctcagagg agcttattgc 540 tgaaaactgt tctcagtaaa ttgttgaaag attactgaaa ctg 583 <210> 67 <211> 845 <212> DNA <213> Gallus gallus <400> 67 ccgcgcttcc tgcccgacac cggagcgcct gcagccagcc ctaaccgcga gactaccgcc 60 gcagcgacgc cggctcggcg cactcaaggt tccggaccta cacacggcga gccgaggaga 120 ccagccccgc ccagacgcgc gcagccccac cgctacttgc cggccctgtc gctctgcgga 180 aaggtctgcg gggcgctgcc gaagcgctga gtccaccatt cttctgcttg acatcagtcg 240 ccacagagag caacagaaac tgcacaaagc aagcagacta cttcatgaag tacacagtaa 300 aacagctatt gagaaataaa gcacaagaga aagaaacaca ccttttccaa tttgcgaagc 360 tttccagtag ataaaaactc atctattttc tcagctattt tagcacctac tccatcctaa 420 acattgagga aggatgaaat acaagtcaat ttcaaggtca cttgcagcca tcacatctaa 480 gaaaaacaaa gctcaacaaa aagaaccacg acgccacaga agccatttta aaacactggt 540 ttgtaagaga ccaagagcat caatcgtaca acacagtgag cacaaacaca gtacttcaag 600 tgctgtatta ccagtcccct gaaaacagtt attttaaaag ttattttaaa ggaatcaaaa 660 ccaggttttt tgattcctac aactccagtt ccccaaactt agcgagataa taagtaacac 720 agcaaagggg aaacctgcac agcagaagtc tttcacaaaa gagacccatc tggagtcccc 780 agttccactt acgaaatcgc cacagctcat gtgctggact cctcgccgtc ccgagccggc 840 tggca 845 <210> 68 <211> 823 <212> DNA <213> Gallus gallus <400> 68 cgcccgcccc tgcggttggc ccaagatggc ggaccggcgg cggcagcgcg cctcgcagga 60 catgcgagga cgactcggac tcggccgcct ccgacagcgg cgactccgcc gccagcgccg 120 cccgctcccg ctccggttcc gcttcgccgc ggccgtcaca ccgccctccg cgaggcgccg 180 ccggggctct gagcgcgggg ccgcggggcc gaggggccga cagcgccgcc gggggagggg 240 cggccaaaag cgctccgcag tccgagtgtg aaagcgagga cggcatcgaa ggcgacgccg 300 tgctgtcgga ttatgaaagc gcagaagact cggaggcaga ggaagaagat tacagcgagg 360 aagaaagtgc caaagtggaa ctgaagcagg atagcaatgg ttcctgtgag tccgcagcaa 420 aagcagagaa aggagatgag aaaccggact ccaaaggtgc tgtaactggt gaaaggcaaa 480 gtggggatgg gcaggagagc acagaacctg tggagaataa agtggggaaa aaagctccca 540 agcacctgga tgacgacgag gatcggaaga acccggctta catcccacgc aaagggctct 600 tctttgagca cgatctccga gggcagacgc aggaggagga ggtcaggcca aagggtcggc 660 agcggaagct gtggaaggac gagggccgct gggagcacga caagttccgg gaggacgagc 720 aggcccccaa aaccaggcag gagctgattg cactgtatgg ctacgacatc cgctccgctc 780 acaaccccga tgacatcagg ccgaggagga tgcgcaaacc gag 823 <210> 69 <211> 594 <212> DNA <213> Gallus gallus <400> 69 ccttcaccaa ggacagtgag cccaggcaga ccgcatgctc tgcattgcct caccgcccct 60 gtgattcgtc tcatccataa tatcacggaa tttgtcatgt tgtactctgc aatcaaacta 120 agggagtttg ttgatcctgc acccatcttg ccaccaggct tggttgttcg ccctggaaag 180 gatgtctgtg gattgcatcc tggacaagtg ggacggctcc accagacata tgtgcctgga 240 cgagctccag ggcctttcag tagactgcaa ccagctccag taaactatga agcccaaact 300 ccatttcaac ctgactttga caattcagct ctcaggaagt atgttcattt tctaaaaata 360 gtgagaaggc ccacagctga ctggtacaac caaacttcct acaaggcagc atttgacttg 420 ccttatctca aaacaggtca tgaaaataaa cacacgccaa caacagttcc tgtaccatat 480 actaccttga atagagcatt tgctcagagg atgttttctg ctgcccaggc ctactcttga 540 gtggaagaat gaaagaaaaa tcttggaaaa ggagcataaa tcactcttgc attc 594 <210> 70 <211> 700 <212> DNA <213> Gallus gallus <400> 70 ctccggccgg gttcgaggga gaggcggtac gggactggca ggacggagcg ggccgcagcg 60 tgaacgctcc tcacgggcga gcgaaggcgg cctcctcacg ggcacctttg gtcttcggga 120 tggggcggta aaggcgcagg gatcaccaga gccggctctt ccgcctttct gaccgggcat 180 cgcggcggag tgcggttgcc tgaagggtct tcggccggcc tcgcagcggc gctggcacca 240 ggaaccctgc agccactgtg atggccagac aagaacctca agagaggaac tatgacaata 300 tgctaaagat ggtggaggat ctgaacaggg acctagaaaa gcttctggag gaaatagaaa 360 aactgacagt gcaggcaacc tggatggcat atgacatggt agtaatgcgt accaaccctg 420 acctgaccaa ttccatgagg cgtttggaag atgccttcct gaactgcaga gaagaaatgg 480 agaaaaaatg gcaagaagtg ctaagagaat cgaaaggcga agagcaaaaa aaggaataag 540 atgaatttag tcatgctggc agaactgtat ccctttgaaa gtctccagtt ttttttttgt 600 attgcaggca tggaggtttc acaaacacca tggaaaacgg attgctgttc atcttactaa 660 gaactatgta tgtgtttaca tttatggaaa tatttttatg 700 <210> 71 <211> 451 <212> DNA <213> Gallus gallus <400> 71 atctggaggc aggactgctg gcaccatgca gggccgcagg gcagacatga accagcagat 60 ccaggagctg cgcaacatca tcgccttgca agagcaaggg aaaaagtttt ccaggcagtt 120 gtgtgaggaa aagctaagcc agaacagaaa tctgctttcc cagctgcatg ggatattgag 180 ggaggacatc cacgcgctgg acgttgccca gaagcatgac cagcgcatca tctctgagtt 240 ttgcagagcg cagaagtact tgtgcggcaa aactgtgcag ggggcccgag agaagctccg 300 aagcagcatc tttgaccggg tgaacacctg caatgcactg ctgcacgagg tgaggcagcg 360 gagccaggcc cgggatgtgc tgcagtggcg gctgcagcag ctgctggacg tcaggatcga 420 tgacaagcag caccaggagc aagtgcagat g 451 <210> 72 <211> 786 <212> DNA <213> Gallus gallus <400> 72 ccttcggtga gtgcggtacg cacctgctag cggctatggc tcgcgcggct cgggtggtct 60 tcatcgccct ggcgttgatg ggaatcgtcc ctttggcaat tctgctccta aacggcgatg 120 tgcatgagca cattcagctg tatacggagc agctgagtcc ggacatggct cagccactgc 180 acagcacgga gcaggggagc caggggcaga gctgggaggc caagggagcc ctgctccttg 240 atgccctgct gcagtggcgg ctctgggtct ttcccggagt ccttatcctg ttctttggct 300 tcctgtgctg cgtcaggaaa accaactatt tgccagagag cagaagcaag tttgggggtc 360 tgtgctgcga cccttgccgt cttccttacg ctcctcttcc gcaagatagc gcaacgtacg 420 ctggccgatg gtggcagcga ccagcaaagt tccagcagcg aggaggagga ggaggaggac 480 gaggaagagg aagaggacag tgacttggac gagacacgcg acgtagacag aagtttgggc 540 caacagcacc cagtggccag cgccacacgc ggccagcaag tgccagctgg tgggaggagc 600 ttggtggacg accttcttcg tgcctgccga ggaaaaacct gccacagctt cgtgccccgg 660 ctgcagccgc ccttcggggg tggcctgcgt ctgtgaagag atggaatgcc caggaagaca 720 acgtcatcta ccgcctgctc gtgcccatga gacctctccc ccgggcaacc ttccgtgtgg 780 gagctg 786 <210> 73 <211> 803 <212> DNA <213> Gallus gallus <400> 73 cgcccatcgc gggaaggcag aagagatcat gacatctgtc aaagagcagg aggccatcaa 60 gaagctcatg gttttcctgc aagaatggga caatgctcac agagttgctc gaaaccacat 120 cctggacaac ttcattagaa gcaatattgg caagacagaa ccagagctgg agctggagtt 180 ctcccaggga gccagcttgt ttctggctcg cctggcagca tggctgaggg tgacctacat 240 gtacagcaca tgcatcaaca agctgctcaa gtccatcggc atcttcttat ctgctgcaag 300 tggccgcaga tacattattg aatttctgga gatgggaggc gtcttgatgc tcctggaaat 360 actagggctg aaccacctga acgaagagga caaaagggag tctgtaaagc tgcttcagct 420 catcgcagac gctggcagga agtacaagga gctcatttgt gaaagctatg gcgtgcaatc 480 ccttgctgag ctgttggcca cctgcagctc ggcagaggtt cgggatgagg tgcagatttt 540 gctggactct ctgggccgcg gcaaccccaa gtaccagaac caagtgtaca gcggcctgct 600 ggcagtgctg ccctgcggct ctccccacgc ccagcagctg gctctgcaga cactgcgcag 660 catgcaggac ctgctggaag agcctccgcc ggctggagtg gcgccgctgc tggcagcact 720 gggcacgaca caccccgagg tgcagtacga agcccgtcca gctgctgctc gccgtcgcgg 780 tcccgggcgc gccccgcccg ccc 803 <210> 74 <211> 685 <212> DNA <213> Gallus gallus <400> 74 aagccgcgat ggcggcggtt gggggcgggg cgcggcgctg ctgggaacgg tagagccgct 60 actggggaaa caaaaccctt ccgagggggt ttgaggcagt catgagaggc acctgagggg 120 aatgggggtg cagggagggg cccggagtgc ggcctttccc tgtcccagtc ccagcggcct 180 gcgcccatcc tgccgcccct ccccccaacc cccttcccgg cttcctgccc tcctggcccg 240 gctcgccctt tcctgtcccc ccctccatga agggaaatcg ctgctggatg acccggccag 300 agctgagcag gagacaaagg gaggctgtcg tgttcctgga ggggccatgg agccgtgtgg 360 ggagcgctga gagctacgtg tggggcagag tgggggtcgc caccatgttc ccagaggagc 420 gcaaggaggg cagcccccgc ttcgccaagc tgcacttccc ggtggggctg tgggggaaca 480 accccaggaa gcgcctggcc caaatccggc accgttggcc cagcgccgcc tccgtgcgct 540 ccaacagctc cgacagcgcc gagccgcgcc agcacggcaa ggccaaagcg gcccggcaca 600 aacgcctctc caacctcttc ctccgcgcca acagcgcccc cgcagggctc caacgtcacc 660 cctggagggc ctccaacgtc acccc 685 <210> 75 <211> 621 <212> DNA <213> Gallus gallus <400> 75 ttgccaaatg gtggccttaa acttccagac atcaggaaca ccaatggaga ctgcaaaatg 60 ggaaattctt agacaatggt ggctgtggat atattctgaa accagaattc cttagaaatc 120 gcaattcaac attcaaccca cacaacgtgg gaagatatag taacccacta tctctatcaa 180 taaggctcat cagtggtcat cagttaccac ccagtaacct gtcgaagagt aacaaagctg 240 accctttagt gcagttagaa atttacggtg tgccagaaga tcaagcaaaa agaaaatcta 300 gtgtcataaa aagcaatgct ctgagtccga gatgggatga aactttctct tttactgtcc 360 aagttccaga actagcactg atacgcttct gcgtacaaga tgagatctct ctggttgcaa 420 atgatttcct cggtcaatac actttaccac tgttgagttt gagtaaaggt tactgtactg 480 ttccgctgtt ttccaaatct ggtggcaaac ttgaacctgc atctctgttt gtgtatgtct 540 ggtactacta ataatgctcc gcaacagttg tcattctggt gactcacacc ccactcaata 600 aagcaacatc acttggcatg c 621 <210> 76 <211> 859 <212> DNA <213> Gallus gallus <400> 76 caccgatgga tgccccacag gctgcccggc cctccagcca cccctccctg ctccagttcc 60 tcagaagcct cctcggggat gagttccttc aaggtctggg tgctgctgat gaagacacct 120 gctctgcaag ccctgagcca atgttttcaa tggatcttga cactgaatgg gagaaagggc 180 atctcctgaa agcagcaaat cagggtgcag gacccccgaa agcagagctg tccctgtgga 240 acaagttcat cagtgtgaac agacaccagt ggactttgcg taaggctcgt gcctcgagga 300 gctcggtgga gctggaaagc gaggagcagc cgctcctgcg ggcagctgag aacagaccca 360 cgagccctga gctccccgtc ctgcccgcag aaccctctgc agcgctgccg atgtacaggt 420 tttccagaag cagagcagcc actggtggct ttgggagaag ccaggcagag ctgggagcaa 480 acggagcagg aatgcctgga gaaggttgag atgcggcacc cgcagaggtg ggagtagccc 540 catggagcca cactgccact gtcctgcagg agacatcagt gcttcccctc ttgccgtgcc 600 caaccttcac aggagccctg tgcttcagct tcctcctctt cttcctctcg tgttcatcat 660 tcatgtgcac actgcatttc ctttctgctt tctccctttc ctgttccttg ggaaaagccc 720 ctgtgtgttt caccaccctg atggggcaga ccaggttttg gttcgaagtt ggtttcaatt 780 gaacactttt gtggtgaatg agctcttctc gcttctccct ctgtgctctc aggttccctt 840 attaaacagt tctctgctg 859 <210> 77 <211> 665 <212> DNA <213> Gallus gallus <400> 77 aacgctcagg ccggcgtgga tggtgagcgg gatgggccca aactgaacca gcgtctcagg 60 aacgcgggct ccctcacctc cccgttcctc gccacagccg cgccctggtc gccctgcccc 120 gtatcctcgc tttccagggc tggaagggtg cgagggcccg aacactgccc gcgcaactgt 180 tggtgccggg ataccagacc cgtaaagctg agtcttcttc ccagattgtt ggttttagga 240 agatgtgatg ggaggaaaaa gtgcatccaa aatcaaagta gaaacaaggg tcgataagcg 300 gatcgaatcg gaggagtcgg aagatgaagg gaagaagcaa gcggtgcgct tagtaacaga 360 cctcagccag cagagcctga gagatgagca gaatggagaa gcagagacac cagtggacat 420 ggaaacaatt agcctggacc cagaagctga ggatgttgac ctgaatcatt tccgaattgg 480 aaagattgaa gggtttgagg tgctcaagaa agtgaagact ctgtgtctgc gtcagaactt 540 ggtgaagcgc attgagaacc tggagcaatt gcagaccttg cgggagctgg atctctacga 600 caatcagatt cggaagatcg agaacttgga ggctcttgtg attttttgag gtcctggaca 660 tctcg 665 <210> 78 <211> 1392 <212> DNA <213> Gallus gallus <400> 78 tgggtttgat gactgattca cattttcagt tacaaatctt ctggttaaat aagtatattc 60 agtctctttg agtattccta ttctcttagt catttgttta ccaaatattt tgctttcccc 120 ttctttccca tcctcagtct cttgggctgc tgtgaaagga ggcaaaggta tgaacatgtt 180 tgctgtggtt aacaagtagg cctcaaaaac cctgaccatc tgtagctgtc actgtctgtc 240 caggatacat gtgctgatgg agaaactttt aaaaatggag ggagtggagg tgaaagtaaa 300 aagggggggg ctcacagaca caaagggtga gggtgccaga tctaagtagt tttctcgtag 360 atttgcaacc actcttgtta ttctaatgct cttgccaaat tgtagtgcta attggcctcc 420 tgatgtgaag taggtatgtc cagtcctcta aagtagttgg cagatctgta gcagcagtgg 480 caatactcca aacagctgaa gcttgggtgt aagaattatc ataacctaca aggaattctg 540 tttttttgtt tttaaaattt ttgtctgctt tttcttttta attcctgtgt tgagtcaagc 600 tggattattt gttttaccac taacagtaaa gctatctcag gcttaacaag accttgatgt 660 agcaggcaga gttactcatt tgaagccagt gagctagcac aaatgtcact tcagaaccat 720 ggcttggtaa aggggttctg atggttgaag aaaatcagct gttgctcttg ggaaacctgc 780 tgggtttggt ggtatctaat catgatctgt gaggccaaga atgttggctt tgaaagctca 840 gggaagaagc agcagggaaa aggaagtaac acagtgtgcc tgaaaatgac aacgaagcgg 900 aatttgttgg tgcggctggt gccatgccgc tgcctgcgtg gagaggagga gaccgtcact 960 acgcttgatt attctcactg cagcctggaa caggtgccta aggaaatttt tacatttgaa 1020 aagaccctgg aagaactcta cttagatgct aatcagattg aagagcttcc aaagcaactt 1080 tttaactgtc agtcgctgca caagctgagt ttgccagaca atgatctaac tacattgcca 1140 gcatccattg caaatctcat taatctcagg gaactggatg tcagcaagaa tggcatacag 1200 gagtttccag aaaatataaa gaactgcata gtcttgacag ttgtcgaggc cagtgtaaat 1260 ccaatttcaa aacttccaga tggattttcc cagctgctaa acctaactca gctgtacctg 1320 aatgatgcat ttcttgagtt ttgccagcca ctttggcaga taactaaact ccagatattg 1380 gaacttagag aa 1392 <210> 79 <211> 547 <212> DNA <213> Gallus gallus <400> 79 cccgctgttg ccattgcctt caggatcagg tgaggaggta agcaggagac gaagaacatc 60 acgaagattt ttgcaggaga taaaatagcc tctggtcgtt ggcttaatac aagggtgtgg 120 gtaaatgaca tttttggcat ataaacaaca cgtacccaca gaaatgctgg aaagagccca 180 ggagcacttg gagcggcccc agaggcacct ggacaaccct ggagagcctg gaagagcctc 240 agagcatctg gaagaatctg aaagctgccc cggcggcccc cagaggccct gggagagccc 300 tgtgcagagc acagaggtgc ccggacagga ctaggaagac gcacatcctg ctcgtcctcc 360 acgttctgag cctgaaggta tcaaaggcag ctgggatcaa tctggaccag acttgctcaa 420 gatttcatgc agctgtgcaa taatcagtaa agacagcttt tcctctagga tgtcatgtca 480 tatcttcaga gtaagataaa aaatgaatac tgccttcttt cagattaaaa agatagaagg 540 gaaaaaa 547 <210> 80 <211> 737 <212> DNA <213> Gallus gallus <400> 80 cgcagccgcc tgaggcggcg gggccgcgcg gccgttatca tggcaacggc gcctcacgcc 60 gggcgtcggt taggccccgg gcgcagcgct gggagtgtcc gttgcgctct ccctcaccgc 120 cctgcccgct gttgccattg ccttcaggat caggtgagga ggtaagcagg agacgaagaa 180 catcacgaag atttttgcag gagataaaat agcctctggt cgttggctta atacaagggt 240 gtgggtaaat gacatttttg gcatataaac aacacgtacc cacagaaatg ctggaaagag 300 cccaggagca cttggagcgg ccccagaggc acctggacaa ccctggagag cctggaagag 360 cctcagagca tctggaagaa tctgaaagct gccccggcgg cccccagagg ccctgggaga 420 gccctgtgca gagcacagag gtgcccggac aggactagga agacgcacat cctgctcgtc 480 ctccacgttc tgagcctgaa ggcttgtctg aatccctcct cttccttctt cagatggttt 540 gagcaaagaa cacatcaaag atgttgacgt gtctctcatc aactaaatac cagcagccaa 600 gttatctaag gtcattttct gcctccctgc cactgggaag aaagtgcctt caggggttct 660 cattgtgtac cccagaagta cacagcataa caacttctga gtaaacacag gcacagttca 720 gcatccccca tcctcgc 737 <210> 81 <211> 811 <212> DNA <213> Gallus gallus <400> 81 tagagtcaga cctcttgcct tgtaggagcc caattgcaat agtttgcagt gaagctaaaa 60 ggataaatga actttctttc agttgtataa tggagggatg gatgaagatg gatgtacatt 120 ttcagcctgc ccatgcttct caaactgctg ttatctgact tgccacagaa gaaaagaaaa 180 atgctgcttg tgctggcgct tcctatttac cagtgagcag aactgcagct gtttcccgtg 240 cccgtacgaa gaagacaaac cttaccagtg ctgccactgc tcatgttcag agcacgccaa 300 ttgctggtgg tgctgctgct cttgctcaaa tgacccagac tgcaagtgct gctgctgtgg 360 tggtgagaat tcagcatgtc aatactatgc aagcaaatgc ggcagaaact ctatctgtga 420 accacaacag tcaacagcac cagagactgg ttcagtcaaa agatgtcaca tcaagattaa 480 gaactagaaa taatgcatgc atcagtatac cagaaaggaa aggctccaac caggcttttc 540 gtgatcagct tgcctgtcca ctgtgtaaac agttatttct ccagccgttt atgcctgccg 600 tgcaatcact gcatttgtga gaaatgtata ataaaaagca agaccaaagc tgaagcaaca 660 ggaaactatt atatcatcat ctgcccagtg tgtaacaaag ctcattgtct cccttacaca 720 aacaaaattc agctgaggga agaattacct taaagcaaaa ctagccaaga aatacatgcg 780 cagacacggc atcctgaaat ggagatttga c 811 <210> 82 <211> 1111 <212> DNA <213> Gallus gallus <400> 82 ccccagtgcc cacactggag ttggggtcct gactgcaggc tgcatcccct ggggcacagg 60 gggagatgga gcatggctga gggagaaata ctccgttcac aggcacatcc cccagggcct 120 ggtggtgatc aacgttcagt agatgcactt ccataggggc tgggtgagac ttcagctctc 180 ctacatcgac ctgctttcct tccttctgaa agcttccatc agcctcccga agcgggcttg 240 gattcacctc tccgccttca tgagcctgaa cgtgtttcag cccctcctgt gtctcctcct 300 ctgcagggca gatcccctgg ctgtccccca tgacagtgcc tgggctgtgg acatcagcct 360 catacccttc cacctccagc acagcctggt cctcccaccc ttcctcatca ggatccacgt 420 gtgtgttgga tgagacctcg ctcctcctgt gctggggtgt gacctcgggg gggctgggag 480 tcctgtgccc cactgcctgg gggaagacct ccatggcact tgctgtccct tgaggctcgc 540 agctgatcct ggtcgctgct cacatctttg cttttaattt tcttcttgag ccgagtgtgc 600 cgtttgttga ggtgttgcat caaaggaccg gggggtctga attgctttat ccgcttttgg 660 cacgcctgtc ctgcgtacct cctgcctggg tgggtacgtc tcagccatca ggatcatgca 720 ctgctgggta ggcctcttgg aggtcctgct ggacacggag gtcctgctgg acatggaggt 780 tctgctggat gttgaggctc tgctggatac agaaatcctg ctggacacag aggtcctgct 840 ggatttggag gctctactgg acatagaggt cctgcttgat gtggaggact tcttggaggc 900 tgccacatct ctggagctgg cagcttcttc caagccaccc tgtggtggct gctgctgctc 960 ggtggccacg aggctgtcag acttcagtgg cttgtggggc tccatctcct ccttggctga 1020 ctctggtgtg aacagaggca ctgtggtcaa ttgattaccc tgctggcctc aagcataccc 1080 aggcagcgtt cacctgtcac taagctctct g 1111 <210> 83 <211> 458 <212> DNA <213> Gallus gallus <400> 83 cacgtggatg aaaactcctg cagtcaaagg agatcctgga ggctatggac cccttgacag 60 gcagccaggc acagccccct gccccccatc ctttgctggt cccccaggcc cccaccaagt 120 atgcagccat ctccacccct agctccccca gcacagagtt gcccaggatc agctcttccc 180 aaagcggcta cagcctgaag cgatccttcg cctgcaccaa cttgctgctg ctcagcatgg 240 ccagccccga tggctctgtg gacagtgagg catccctcgt cccccacgta cacatgtctt 300 gctccaagga taaatccaag cccaccacct ccacggagaa ttgatcgtca ccagcacgac 360 tgtcccactg aagaaggcag cgtgtgctgc acagtgagca ggaccagcct cccactgcag 420 gacatcacag tgctaaaagc gtgttgcttc tgtgcaat 458 <210> 84 <211> 343 <212> DNA <213> Gallus gallus <400> 84 gcagaagggt ccgcccccct gagaggactg ccactgagcc cggacccttg aatccctgtg 60 aagatgttct ggctcctagc caagttattc ctcattgttc agctgcaaac agtcatgaaa 120 atactgggct tccgcacttt tgggagacaa ctctggtcat cagctctgat agttgccctg 180 tccttgcaga gggtggagct gcagaagagt gctgtgagaa ggagcacacg gagaagcacg 240 gtccggaacg aaactgtgca gaggcacatg aggaggtcaa taaggattgc aaaaaatcaa 300 gagagattga gacagcagat tctgcacttt tggctgccct gtg 343 <210> 85 <211> 700 <212> DNA <213> Gallus gallus <400> 85 tgaggaacgg agcggagctg tacgcccgcc ggtccccgcc gcctgcccgt cccagagcac 60 caaggcaaca gcacagatag ttcgggaagc agtttgggaa gcactgtcac agcatgtaaa 120 aagatcctct gcagtaatag cctgctagaa tcaacagact actggctgca gaatcagagg 180 acaccatgcc aaattggctt tctggaagac aagtcagaaa gcaactgtgc ctcaaattgc 240 tcttcccacc ttgggtatgc cagtggtgta tttgggtggg cacggtgctc gagcaaacga 300 gggaacagag gacacttaac cactatatga ggattaagca ttagttcagc attttactca 360 gaaagcattt cgttctgagt cctttccagt gtctgatgat aaatgagtgg aggatgcagg 420 aacaaaagaa gtacatgaac gtggagcagt ttccctagct tatcctatag ccagcaaacc 480 agactgccat taacagaaaa tacattcttc tcttctcttt catggagatt ttatgaattg 540 ctgttgccag cagtgccaga gatgttttga agttgccatg acgtttgcag aatgaaaaaa 600 gaaatctaag ttgtactact tcactgatgg gtacttttct taaagataac tagtaacaca 660 tttagctata gtgttcagtt ttcgtttgga agagtttatg 700 <210> 86 <211> 683 <212> DNA <213> Gallus gallus <400> 86 caaaaatctg acagataatc caggtccttg tgccttttga ggactgatgg cccctgcttt 60 ctcctgcaaa taagaagaca gcttgctcgt tctctgtagc agatagaatt gcagagcctg 120 aagaattctc agagcagcca gaagatccgc ttccaccacc accaccacca ccacctcctc 180 cgccaccgcc gccaccaccg ccacctgcag ctcccatgct accaccatct ccatcagctc 240 cttcttttca tttatctcag atagatgcca catttgtaga tccaagtatc ggattattga 300 tgcctcctct ccagacccct agcaatcttg ccgttttttg tgatcacaat tataccgtag 360 aggatacagt tcatcagcga aaaagaattc agcagctaca ggaacaagtt gaaaaactga 420 gaaagaagct caagacagcg cagcagcgat gccgacgtca ggaaagacaa attgaaaaac 480 tgagagagat tgttcagttt cagaaagaaa aagacatcct ggcaggaaaa ggctatgtga 540 ttctgcccaa tgactatttt gaagttgtag aagtacctgc ataaaagtca ccgagcatcc 600 atttccactt tgctaggcta agaagtttag tttaaaaact aaatgctctc taattctgtg 660 tgtgagactc ttccagaatc cct 683 <210> 87 <211> 905 <212> DNA <213> Gallus gallus <400> 87 gcagaattac tgaaagtcag agagcataag gataagcagg aaacgcatcg atcatccttt 60 gtacaactta ctgagcaatc ggaaaataaa aatgctgatt tacaggcaca gagtcaagaa 120 agccaacggg aagtgctaag caagataaga aaccactctt tatcaaaagg agccgtccca 180 ctgctctgag caggaaagaa taagaacaga aatctctgac ctgacagaag agcttcatca 240 gaaagagatc actatagcaa ctatcatgga gaaagctagt cttctggaaa gacggttaaa 300 aacggaatta cagataaaag acaaattgtt agcaaaacaa cagttgttga atttcagata 360 caaagttatc aaatctgaaa acacacatct gaaagagatc atggaaaatc agaagtataa 420 aagttgcatg agtatcgatt tatctaacaa aaaacatgga gatttcacag cttctattca 480 caaactcgag catgagaatg caagattaca aaacagtctt gttaaaggac agaatcgcac 540 agaaacattg atgctgggtg gcatggataa acatggagaa aaggagcaca gctaccaaac 600 ccactcaaag atgcaagaaa aggaagagag aactttccaa aatgaagatg aaaggcatat 660 gaaacttcaa cagactgcta tattcacagc cagtggatac catagaaact gtagtcctac 720 ttcacatggc caaggtgata tcattggttc caaaagtgat cgcgctttat cttatcacaa 780 acttgacaga ggtcgtcaaa tggaactaga tgataaatct ccatccccac caacagaata 840 tcctttgtgt tttggtggac tgttcctgaa aaagcagaac gggcacttta ttaagtcatg 900 cttac 905 <210> 88 <211> 705 <212> DNA <213> Gallus gallus <400> 88 ctgttctatt cttacacatt taacatcagt gaagcttctg tttgcttgat gctcattagg 60 aagaatgttt tttattttac tgagatagta tttaattgaa actttaggac tttctcctcc 120 ctaggttgtg gaaggcctgc ctctttcaaa tggaaggaag ctgcagtacc ggatcaatgg 180 gttttatgct tttcttttga ctgctgcagc tattggaacc ttactgtatt ttcagttcga 240 acttcattat ttgtatgatc acttcgtgca gtttgcagtg tcagctgcag ctttttctat 300 ggcattgagc atctatttgt acattcgatc cttgaaggca cctgaggaag acctaccgcc 360 aggtggaaat tctggatatc ttgtttatga cttctttact ggacacgaat tgaaccctcg 420 tattggcagt tttgacctca aatacttctg tgagttacgt ccaggattaa ttggctgggt 480 tgtcataaac ttggcaatgc tcttggctga gatgaagata cacaatcaaa gtatgccatc 540 gctgtcaatg atactcgtga acagctttca gcttctatat gtggtggatg ctctttggaa 600 tgaggaagct gttttgacta caatggacat tacccatgat ggatttggat tcatgctagc 660 ctttggagat ttgggcgtgg gttccatttg tctacagcct acagg 705 <210> 89 <211> 262 <212> DNA <213> Gallus gallus <400> 89 cggccaatcc cgccggcagc agaagctccg cgtcccgccc gccgcccatt gcctctcctc 60 gaagtaaagc ccgccttttt cccgcagccc cgcccgctga gggccgccgc catgttgcgc 120 ggggcctcgg cgaggcgggg acagcggatg gggctgctgc gctggtacca aatcccgctg 180 tcctcggccc gcgtgccccg ctcggggctc tttagctctt ccttccagta cagcctttgt 240 tcctaccagc agttccgtgt ca 262 <210> 90 <211> 811 <212> DNA <213> Gallus gallus <400> 90 agctcacctg aacacttctg tatggcatgt tgcttctgtt tcagccagca tcttgagtgt 60 gggaagatat ggcagtgcct gtaaatcagc tccgtaatga agttctgccc atagtctttt 120 taattatact cttcgtttat gcattcttta cttctgagga tgctttgtgg tgggcagttc 180 tcttcctcat agtttttgct atgggaagcc ttttccgttt tgataattaa cctttaaaaa 240 gcaagcaagc aaacaaacaa aaaccaacaa aaacaatcga acctcttaag aaaataaggg 300 aagaaaacag attctctcct gtttttaaat cttcaagaca tcaaggaatt tcagttcaag 360 gaaacaatag gagagacctt gtaagtgact cctcttgtga agtgtgcaag gatccaccct 420 acaaagttga agaatctggt tatgctggtt tcattttgcc aattgaggtt tacttcaaaa 480 ataagccact tcacaggtat tccagtacag acaaagagga gctagaagga ggaagaggag 540 acagaccatc atcacttatt caaggaagaa cctaagaaag ttcgatttga ctatgactta 600 ttcctgcatc ttgaaggcca cccacctgta aatcacctcc gctgtgaaaa gctcacattc 660 aacaacccga cagaggaatt cagaagaaag ctgctaaagc aggaggggtg agtcctgcac 720 ccatgtcaaa agcagttaca ccggatgttg ctgctcattg ctggtgcatg attactgtaa 780 taaaggttga ctgggcagtg aaacttttaa a 811 <210> 91 <211> 297 <212> DNA <213> Gallus gallus <400> 91 cgggcgttgg gatggggcag tcgaggtggg gcgggcggca gactggggtc ttgcggcctt 60 ctgagggacc aggaagggtg tcgtgcaggg atggctgcct gaggagcgca tcgaggcggt 120 gttgttcttc acaagatgct gcaaggagtc caccaagccc cgcagcagct cctgcctcac 180 gaggtgagcg tgggacagaa ataaaacagc aaaaggcacc agctagatcc ttttgaatgt 240 ccttgtagca ttacaggcat gtcagtgctg gtcacaagcc attatgggac aagccga 297 <210> 92 <211> 819 <212> DNA <213> Gallus gallus <400> 92 ctgcccatta atcggtggtg tgaggagcac aggagccctc cttgtggctg aaggaagagc 60 acagcagatg catataacgc taagagctaa gagagacatg cttcctacca gcagtgattg 120 aaaccagcaa atggagtgca ccattagcac catggggtgc aacgcttccg cgcattacag 180 taaacacaaa tataacgaaa cagccataaa agctactgtg ttaatccaga ggtggtaccg 240 ttgtaaaaga gcccagctgg aggtccgtcg ccgctattct ctgactatct ttcagtcaat 300 cgaatatgct gatgagcaag agcaaacaca gctgtccaac tttttcacgt tcatgctgga 360 gcactgtgtt catcgtggtg aagttgctcg tacttccacc ggctccgatg tttctcctgg 420 tacggactca tatgtaatta aatatgaaaa gattgatgtt ccagactcct atcgtggacc 480 acgactctca tttccccttg atatttcaca tgctaagact cttcttaacg cgctcaagga 540 tagacagctg cttcatgccc gctatgtcct gcagctgtta tgtgagacca ggagggttct 600 caaggagaag ccaaatatca cccatggttt cagtttccta cttcaaggag ataaccatct 660 gtggagactt gcatggaaat ctggatgatc ttttactggt attccacaag aacggtctgc 720 cttcagaaac accccttatg tctttaatgg cgattttgtt gacagaggaa aaaatctatg 780 gaaatactat atcctattgg gtttcttctt atctacccg 819 <210> 93 <211> 507 <212> DNA <213> Gallus gallus <400> 93 cacggctgcc acgggattgc agaggccggc ctggagctga caagaccttc ctgctgaact 60 gaagaagtat acgtgcagca acttctgccc agatcacgta gaatgtgcat gaagacaagc 120 atgaatgcca tagctgtagt cagaagactc ttccttgtcc ttttccagtg caaaaagctg 180 ctgttcttgc tctgtgtggg tctgtgcgtg aagagcctgt accatgtgac aaacacactt 240 ggcaggcata ttgaaaatgg agcgctttac agagtctttg gaggggaccc attcatggac 300 accgggggtc aggagctgga aagcaggaca acatggaggc acgttggtgc ttgccggaga 360 cgcttcatgt ggagaaagcc ttttcttagc aggtttgaat ctcactggga gcctttcctg 420 accagcaaat ccatatactc actattggac aggcctttta aacaggagct tgaggaagtt 480 ctgtggctcc ataaacttcc ttttggg 507 <210> 94 <211> 831 <212> DNA <213> Gallus gallus <400> 94 gagatgatat gtcgaggtca gcgaggtcac cgaggtcttg agcacgggga cgctgctggg 60 gctgccttgg ttggccccct cccatctcag ggagcagttg aagagcctca cggcctcctc 120 cagcagcacc tcgtcgggca gctctatgtc taggctctcc aggttggccc ccatctcctg 180 ggggttgggg acggcagagg cgcccattgg ggtgtcacga tggaggggga cgatgccacc 240 catgggcgca gcgctgctgc cactcagagg ggcctccgag atcctcatct gctggatctg 300 cccctccagc tgctggaagt tggcgaccga cggcagcagt ggggtcacgg ccgtgccctg 360 ctgcttgtag ggcacaaact gctgcggagg ggccgggggg gtcacccagc tctgcggggc 420 agcggtgggc agcgaagcgg agcccgcggg gttggccggt gggggcgcgg tggtggtggt 480 ccactcgaag tgaaagtcgc cgggggtcga agaagcagcc gcacggagcc atccacagcc 540 ctgcgggggc aaaggggagc tgggcgggct cctctgggac cccccccaga gctcacactg 600 cgggtagggg gatgtctaac ctggcggggg accccggggg tgcgccgggt gccatcagcg 660 tccccatagc cggcggtgcg aggaggggct gctccatcgc agagggctgg cagggtttgg 720 ctgagcctgc gaggctgaag agctccggaa ggatgagcgg cggaggcgcc ggggggccac 780 gagtaggagt gccaggcggc agccaactcc atgcgctgct gtcggccaga g 831 <210> 95 <211> 878 <212> DNA <213> Gallus gallus <400> 95 ttttgataaa tccctttttc aggaaagcct tcaccactta agccactgct ttgctcttta 60 cagattcttt gtactgatgt tctggaaggt gacccatagt tttgctgtgg tttgttggct 120 aagacttcac gaacttgaag aaacacgact aagcagactc ttttgaattt actgccacat 180 aacacaaata caagagaatg accatggttt ctcatggagc taaaaggcgg gctgccatgt 240 cagttgaacc aattcacaga atgcatcacc aacagtatca aaatctgtta aagaagatgt 300 gcatgccatt tatcagaggt cctgagaaca gacatgattt tgccagcttt gaagagaaag 360 acagcaattc tttccttaaa ttccatccag acacccctcc gtttgggcca aattacccct 420 tattcccaca ccgagatgat gtgcctttag tagatccctg ctcaggcttt gtgagtgtag 480 gaggggatgc tgagctgcag cctggtgttg gaaaagttat tgaatccctg gtggactaca 540 gtgatgtgaa acctcaccag cgggtcccca cagcaggaaa gggggcccgg accgcccaca 600 ggaggcatat gatcctccta gaggaaacca gccaggaccg acggtggaac tccagggctg 660 tgccagctgc tttgatcagg gcaaaactca gaggtcagac aacttcagtg aaagggtttt 720 cgtgctccac ttgaccaaca ccataatttt gcattttgta tggatcccaa tctcaaggaa 780 tcaaatgatc cttctgcagt tggagagaag aaaagcaaag gactattttt acaagtcaag 840 tactcaaaag gcatttggaa gaatttcatg gggataat 878 <210> 96 <211> 893 <212> DNA <213> Gallus gallus <400> 96 agcaatgcaa ccaaaaaatt acaaagatcc ttccagttca gagtcagaaa gtgaatattc 60 agcatgtacc tataagaggg agaaatcaaa agtacagaaa tgtgtgaatg agaaaaacaa 120 agatggtaat catcagaagg atctccagaa tttggtggag ccaaagacag tagcaacctg 180 tgtaaataaa gcatttatta aatcaaagaa ctctacagag caaaaggaaa ttgaaatgtc 240 ttcacctgag agtcctgagt ctctagagac aatgagatgt gctgagagaa tatcagagga 300 atgtgttact caagaccata cttctagtga ggggtcacct ggtcttcaga aatcaacacc 360 agaaaaaaat gaaacatcaa actttgagaa aggaatctct cccagtaatc tctgtttaca 420 aaagaagaat actcaaaata tgtatctaaa ccagtctcct gaaatgtctg ttaaaaagtt 480 aacatttgga aataagagtt tctcaccagt tctaactgct tcatatttgc tcacttaagt 540 tcagtaaccc cacatgaaac atactgtgaa aaaaacacag agggatctgt atctgaaaca 600 catgatgctg atgaaaactt taagttttag acatggcttt tcagacaaaa tttctactga 660 aggaaaacaa caagacatta ctaaacctgt cataagaaat aaaggagagg aattatctcc 720 agtattcttt ggtccaggtg gaagtgaagg tacagtccct ggagtcaaga cccttgcggc 780 cctacacttg gagtcagggc cgacttcacc atgtattttc tcaaacgaag ttttcaacgt 840 gacacagaaa gccattcttg gatgaagtag aacagccaag aggaggaaaa gaa 893 <210> 97 <211> 836 <212> DNA <213> Gallus gallus <400> 97 atggtggggg gtggcagagg agcagggctc accaggctgc cagggtcacg aggatgggga 60 tggggcaatg cccacccctg gggggcgcag gggccgtggc actgatccta atcctctgct 120 caggggggga aatgcttccc acccagctcc ccagtgcaga acctcctcca ggctttagtg 180 gagcatcgag ggtcatctac tccgaagtga tggtgccgag gcagctggag ctgaggcacg 240 gggctgccgt gaaggacgac gtggcctatt taatcgtggc agagggaagc tactacgtca 300 tccacctgac gcgggcaaaa aatctggtgg ccaaggacct cccggtttac acatatggtg 360 ccaggggaga gctgattaca gagttcccct acatgcagga cgactgcttc tacgagggct 420 ttgtggaagg gtcgccaggc tccatcgtgg ccctcagcac ctgctatggg atcaggggaa 480 ttctgcagat cggggacctg cggtaccagg tggagccggt gccgaactcc accacgtttc 540 agcacctcat ctatcggaca gcgccggcag aaggctccgc cccgccgtgc caactgccca 600 tggaacaccc aaccaaggat ggagaccacg ccaatgggag caggaaaatc ctcatcatag 660 agggaggtgc ccggtctcga gagcctgccc gtgtccacca gatacctgga attggctctg 720 atcaccaaca aggagctgtt caaagccaac aggcacaacg agaccctgat gctgcacctc 780 ttcatttcca tcagcaacat cctgaacacg gtctacaagc gcatgaagct gcaggt 836 <210> 98 <211> 714 <212> DNA <213> Gallus gallus <400> 98 ccagagcact gtggggcagg tggcagccat ggcttctggg gtgcctacct ctctgcagcg 60 acagcggtat cgccaccggt gtgccagagg ccgtgcctgt gttacccctg atctgcctcc 120 tccctgggag ccctacaaaa aaagaccaag ccccagggct cagctgacag tcactgtctc 180 agctgtgtga ggccatgacc ctgcgagctg tcttgttcct gatcgccgtg ctgaccgcct 240 tattctgcca acacgtctcc aagcacaagc ccacctccca gctgggcggg gtgatgcgga 300 ggatgaggct gacacaccac ccggggagat acctggacaa cacgtggaag gtccctcttc 360 gtgcacagga gggggaaggc tcaggccagg tgatccagcg tgtccgtcgc tcctggctgg 420 cggactacct gggcgacgtg tggtttcact tctcggagag cttccccatc gccgtcctgt 480 acatcttccc cgtcaccgtg ctgatcctgc tcttcctctg ctgcttcacg gtaggtatgg 540 agtgaggcga cctggaggac tctgagagaa gcagcgtggc tacactgaga gagattctgg 600 tgcctcgatg gagctcagct cagagaagcc tccgaaactc caggagcccc gaagcggtgt 660 ggaaggccag gagcaaacca cctttctctt cctttcagat gattttaaag attc 714 <210> 99 <211> 773 <212> DNA <213> Gallus gallus <400> 99 cagaaaatgc ttgaaagcag gaaccacaga gctctggtca ggtatgctgg caaaggagct 60 tggtatagtg caagctcagg gtagaggagc tgatcagttc agatgcgctc tgacaatatg 120 atctgcttct ctgaagttcc caaatttttc ttgacgtagt tgtctcaagg gattgctgct 180 gaagaagttt tctgctctac agcatctgct gggaaagacc tttttcttac caaacatgcg 240 tccactgtcc cctgtgcctt tgcactcagt gccgcccctg aagacatgcg tttgtcaact 300 gtgctcctgt ggatgtcacc gttgtccaca tctgctcacc aaaccctttg agcaagagtg 360 agaagccatg tgcactgtca gtagtacatg gagaaatacc ccctttatcc caacactctt 420 cccagacact ctttcaaacc aaaagcaaag atcgagatgg caaacacacc catggaaggc 480 acttcaatga caaagagaga ttatgtagct catgaagtgt tgccacagaa aattaaagaa 540 cctgaaacgc atgtcaagag tgatgagagt atggatttga cctctgttta taaacaagac 600 tataattcct accctatctg tcaagtacct ccttgcctcc cctgtgagac aagagaaatc 660 tccagtgcca agatgaacac aaaatccact tataaagatg actatatgct ggggaatgaa 720 ccaagacaga gctgatcaga ccaaatcatg ggtatcgccc atcagagcaa att 773 <210> 100 <211> 823 <212> DNA <213> Gallus gallus <400> 100 cccctggtgc tgaaaaggct gttgaagctg gtagccgtta gtctgcagcc agcaatgttt 60 gcagcccccc ggctctggaa ggttctgtgc aggtggtagt ttccacagca gctgagtctt 120 catcctcgag tgctgtcgtg ctcctgtaaa gtttgttaga ctgacatgaa gcccttcagg 180 aaacacatga agatggatga gacctactga aacttcttcc gcgttctctt tcctctggaa 240 gtcttaccag tgtatatcac tgcctgattg tggtaactca tctgtctgca ttgaatgcat 300 tttcaaaaac aacgcttttc cattgccagt aatagtaaca gatctgctct gatgctagag 360 tatcagcaag tgaaaagtga agaaaacttc tctactaaaa cttttaatgt gatagaaaat 420 gttggcatga cctctgctga cagaaaagaa gttctcctca acagttttta agatgcagag 480 caatgagagt tcagctaaaa agctatatat gcagaagcgc ctctcaatta ctcatgttga 540 cacagcgttg gtttcaggaa tttctgaaaa ttttaagatt actgaagaac ctgaagcaca 600 gattttaaac tataatttag agataccacc tcaagcccaa gtcatatcag taccactttc 660 ttgtttctca cctataatgc agcagctgat atctaccaga attaaacgtg tccctgaagt 720 aagggaggct acagaaatac caactgttac ttatatttgg ccagtattaa agataggaga 780 ggcttgcaaa cactcctggg acagacttcc aaattcttaa tac 823 <210> 101 <211> 789 <212> DNA <213> Gallus gallus <400> 101 aacggctgag gcctgcagat tgcagccagc caggccacaa ggcgagggcc aggccaggct 60 gggagcacca aggggtgcag aagtgggacg gggctccctg gctggccagg gggagggagg 120 gctccccaag gttgggcaag caagttttgg aatcatgaat gtgaatttga atgaatttga 180 aaccaattca gagaatactg tacgcggcac agaggaacaa gacaatgcca tctggaggag 240 gtacaatgag agaagcagcc tcgtacggca gtacctgcaa tccgacctga gcctccacct 300 ccttgagacg catcagaaga gagtagagct tctgaagaag tgttgctatt acattgaggt 360 tctgcccacg tttttgatcc tgagagacca gaatctcttg gcgcttccta ccagcatctt 420 ccaagtaatt gacccctgga aattcctgag ggtgaagaaa ctgggaagat cccaaactga 480 aatccacctg cagcttttaa cccacctgtt agaagagctg caacaaggtc gggaggagct 540 ggtctgctat gtagagacct gtgacatggt aactttcctc tccaagtggg acttaattga 600 gcagagaatg tcagacctct ccaagacaat gaacgatttc ctttccattc aagtgtcaag 660 aagactctac accaagcacc gtttggtgtc atgtgcagat attagaggta gcaaaatacc 720 agacattagg ctttttctct gtgcaaagat gccagttatg tttgatcgaa acgaatcatt 780 tgcacacaa 789 <210> 102 <211> 793 <212> DNA <213> Gallus gallus <400> 102 cctgtgggac cccgttggct gctccgctgc cgcctcgccc ctcctggctg ctgaaggaac 60 ggcaggagca ggagttggtc ttatccatag tatcaggttt tgcgtaaggc taagaaatat 120 atccaggagc tggagcaaac cttgagttct ttgctgaaga tgaaagagtc cttcagcctg 180 gaggatggga acccgtccag cctggaggaa gtcagggaag aatatgtcaa gaggcacttc 240 agcaatcaca gcaccgcgtc gccctcggaa gctgtgagtg agagtgattc tgccgtctgg 300 tatttgatgc aagaatgtga aaaacaaaca atggaagaag acggaaagcc aggatttatc 360 cagtacccag acacctcatc cccagatctg gtggaatttg aacggtacct gtatttttat 420 aagcacacag tagagctact gagagagcat ggaattgttt ccgctgagga ggtacctctt 480 cctgtggtct ccacggctat ttcacacctc tggcaagagc tttctgaaga caggagagac 540 agcatcttgc agtactgtag ccagagagat ttccgcctgg gtcctacgga tgcttgccaa 600 gagcctgcgt gcactgatgg cgatgtgagg gacagtggag gtaacagtga ggaagccagt 660 ggttccttgg tctccacacc agaggaagta atgtttgaag acgcgtttga tgttgctgct 720 ggtttccttg aaacaaacga gactcaggga ttgtctagcc aggattcttc atttacaagc 780 ggcattttct gac 793 <210> 103 <211> 643 <212> DNA <213> Gallus gallus <400> 103 cagcctgggg ccgcctggag aagcagcgct gtgtgcgcta tgtgggccac actgctgttc 60 ttggtgctcc tcctgcctcc cacagcgatg atcagaaagt ttattttccc ttttggtgta 120 gatttcctct ccactgaggg agatgaagag gagaagattt ctgaggaagc aaagtcacta 180 gaatccagat ttggacccca gtatcataac actgccctac agagctacag tgaggaacag 240 cctttgaatg aatccagatt tggaccccag tatcataacg ttgccctaca gaactacagc 300 gaggagcagc ctttgaatgt gtcggtgaac gctgatttga tccaagggaa cacagcctga 360 agaaatcaaa aaagaagata ttaaagacag tgcaattatt gctgtgtcat ctacagctct 420 cgccatctac ctaatattta tgctttcttt atgtgtggtt cttagaagac gcagaataca 480 aaacgggaat tcttggcata cctttggatg ctcacgtgaa cagcagaaat cagcagaggc 540 tgggacgaat gcacatggct cacagaggag atcctcagac tcagaaactg cacctctcat 600 taactcaatt gatgagctac tcactgcggt tgcatcaaat tcc 643 <210> 104 <211> 487 <212> DNA <213> Gallus gallus <400> 104 ctcccaggaa tggaaggagg ggatgttgtc ctgcaccctg ctccaggaaa ttgggaccaa 60 gtgcatgtgc atggatgctc atagcctgcc atccaccaaa ggttattatt gctctgctct 120 gttacccagc atttctcctc atgaacagca tacaatcact tcacgacctt tcgtaaacag 180 agaccatcaa gctgtcaaag tcccactgga agtattcacc tcggatgctg ttcctcactg 240 gcacttactg cacccgattc acactaaagg agttgataac actggaaaaa gaaaacccaa 300 agttgaaaca aaaatgggag acatcaagcc tttaagattg caaaccttca taaggctgtt 360 ctttggatcg tgtgctgtta ctgccagtaa ctgctcatcg tgaacctcac cccctgccat 420 ccccaggtta tcataaccac catatcactg ttatcttcca aaacagaaac tatgttgcaa 480 aatctgc 487 <210> 105 <211> 585 <212> DNA <213> Gallus gallus <400> 105 aggaaatacc tgactggtac tctagacctc tctgtcctct ataatggtaa ccaacctttg 60 ttaagaggaa agcctcaaac tttcccagtc agtaacaact gcatgcccag gtctaccttg 120 cctctgcaga cccttaagat ccagctcagg ctagctgaaa ctaatgagat attttcacta 180 cctcaatgcc agaatgactt gactctgaag cagcttaagt ctgatttaga attattgact 240 gggatcccct ttcatttcca gcggcttcac tacctggatg aaactggtcc agctgggagt 300 tacagaagag tatgctggca cctctccata tgcaaagata ctgggaccag agcagaaaaa 360 gggagtgggt agcacacccg tgcttttgtg gctctgtttg ttgccagcca cagaggtcat 420 gttaacactg tgaagtttct cttaagtcat ggtgcagatg tgcgctctaa aactccactt 480 gggaaggact gcgctgcatg tagctgctgt atgggccgct gtgagtgcat cgagctgctg 540 gtgagctttg gggcacgagc ccttgaccca gactgcgagg ggcaa 585 <210> 106 <211> 852 <212> DNA <213> Gallus gallus <400> 106 tattttgtaa gctgccaggt gccagccaac aggccatcct ggacaccctg caactttggc 60 aatcacgagc tgattctttc ttccatgacc cacttctggc tactaaggga gaagactgca 120 acatgcaatg ggaactactc tcatctgttc ttcttgattc tagatgatgc ctttggtgtt 180 ccctctccag atgctcatct ggatccagat tttgcgcctc aggggaaacc cagaggccct 240 cttggtcaat ctgcagagtg gaatcctgac cctgagcctg gtatggatgc tggagaagtg 300 cccccaggga aggccttgcc aagtcctcgg ctctttcctg tgctggagcc ccgctggaat 360 cccaggggtc tggaaaaagg aaaggatgca tctagaagag gaaaatactc acagaggtca 420 tctcgtgtga taaaggaact gctgaaggaa atggggagaa ggcagcgcgt gggactgacc 480 aacaaactgc acaggacgcg ctgcaggaag gcagccatcc cacgctgcca gtctgggaca 540 gagaaacaca gacagcgcca acaactggca tgccagggat gaatgctggg aaaaacacaa 600 gggctgcggt tcaggagttt gtacaagtgc gcttcttccc agtgattgca ctgcttttaa 660 cagtgctgct gtctgcgctg gtgatgactt gtactgctgt gtcaatggat gtggcaaaaa 720 tatccagtgt gcaagagagc agagacaact ccaggacaag ctcatcctga cagtgtgcgg 780 gagagaggcc actctggagg tgaaggcaga gtggagctgg gagaggcaac aaaagaagat 840 gtagttgccc aa 852 <210> 107 <211> 502 <212> DNA <213> Gallus gallus <400> 107 cacgccaaga tgtgtgtgcc cagcagcacc ggtgccggag ccgtccccag cacactgcgt 60 atgcagcggg acaccgtaca gtcacagaat cattaagaaa gaaactccga tctgaaaatc 120 cttgttctct gactcagctc catcagtttg atggctgcag ttttcagaag accttggaat 180 gaaggagcca cactccttca ggaagctcag acctcactct ctggagacga gcatgagatg 240 ctttaatttc tctcatcatc agctacacat acagccccca acagtggaac aaaatgcttt 300 acagaaatag caacgtcatc accacagctt cccaccagcc gttgcaacac actacctgtg 360 tgtgttccat attataaaga aagatttttt tgtgtgtctt ccatagaatt tattagaaga 420 gagttatgtc gagaaaagaa gccacttcat gaagttatac agaaatctct ctatgtctct 480 ctatacaata aagcccaatt ac 502 <210> 108 <211> 709 <212> DNA <213> Gallus gallus <400> 108 ctcacggcgg ttgaggcgcc ccgcggggct ccgttttgca gtttgacgtg gacaaggagg 60 cgggcgagcg gcagatctac caccgacctg gtgaccccca acggcctcaa cgtccgcaag 120 ttctcagcca tgcatgagtt ccagaacctg cacgcccaga gcaaggcgcg ggtgcaggag 180 tttgtgcggg ggcacttcta tgggcacctg gacttcgacc tggacaagac gctcttcttc 240 ttcatcgcgg ggcgctatga gttctccaac aaacgcgccg acatcttcct cgaggcgctg 300 gcgcggctca actacctgct gagggtgaac ggcagcgaga cgacggtggt ggccttcttc 360 atcatgccgg cacgcaccaa caacttcaac gtggagtccc tgaaggggca ggccgtgcgc 420 aaacagctct gggacacagc caacgcggtg aaggagaagt ttgggaagaa gctctatgag 480 tcgctgctgg tggggaacct cccggacatg aacaagatgt tggaccggga ggacttcacc 540 atgatgaaac gcgccatctt cgccacccag cgccaatcct tcccccctgt gtgcacccac 600 aacatgttgg acgacgccac cgaccccatc ctcaccacca tccgccgcat tgggctcttc 660 aacagcggca acgaccgcgt gaagattata ttccaccccg agttcctgt 709 <210> 109 <211> 1495 <212> DNA <213> Gallus gallus <400> 109 cccggcgcgg gcaagaagct gccgcgtctg cccaagtgtg cccgctgccg caaccacggc 60 tactcctcgc cgctgaaggg gcacaagcgg ttctgcatgt ggcgggactg ccagtgcaag 120 aagtgcagcc tgatcgccga gcggcagcgg gtgatggccg cgcaggttgc actgaggagg 180 cagcaagccc aggaagagga gctggggatc agccaccctg tacccctgcc cagtgcccct 240 gagccagttg tcaagaagag cagcagcagc agctcctgtc tcctgcagga cagcagcagc 300 cctgctcact ccacgagcac ggtggcagca gcagcagcga gcgcaccacc agagggacgg 360 atgctcattc aggacatccc ttccatcccc agcagagggc acttggagag cacgtctgat 420 ttggttgtgg actccaccta ctacagcagt ttttaccagc catccctgta tccttactat 480 aacaacctgt acaactactc ccagtaccaa atggcagtgg ccactgagtc ttcctcaagt 540 gagacagggg gtacgtttgt agggtcagcc atgaaaaaca gccttcgaag cctcccagca 600 acatacatgt caagccagtc aggaaaacag tggcagatga aaggaatgga gaaccgccat 660 gccatgagct cccagtaccg gatgtgctcc tactacccgc ccacctcata cctgggccag 720 ggggttggca gtcccacctg cgtcacacag atactggcct cggaggacac cccctcctac 780 tcagagtcga aagcgagagt gttttcgccg cccagcagcc aggactcggg cctggggtgc 840 ctgtcgagca gcgagagcac caagggagac ctggagtgcg agccccacca agagcccggc 900 gccttcgcgg tgagcccggt tctcgagggc gagtaggcgc ggcagtcggg cggcgtgctg 960 cagcggcgtt cactgttgcc ttgttctgtt ggggttgcgg gggggcgttg ggtttcttct 1020 ttccggggcg gggggggcac ggcggggccg cggccgggcc ggcggggcgg ggcggggcgg 1080 gacggggcgg ggcggagccg cgcgggggcc gcagtccggg ccggggccgc cgtcgggtct 1140 cggcccgctc ccgtcggggc ggagcgtccg acgatcggcc tccacgaaac gcggtgccgt 1200 gatgtgtttg tagtggttcc tcgtaggctc cagacgtttt ctcctcgtat cgccaaatta 1260 acgcgttttg catattacag ttgagtgcct cgacttagat tgcaatataa gcggccagca 1320 aacaagtctc aaaaaaaagt tacgtgcgtt tctgcgagtg ttattttgtt aagaacggct 1380 cacagtgtcc tcttcctgtg ttacagaagc caacctgaaa tgaaactagt ctggaaaaat 1440 tcattgttct ctgtagttgc agctgtacct gaaataaaaa tgttattgat gactg 1495 <210> 110 <211> 1219 <212> DNA <213> Gallus gallus <400> 110 caactccgtc ggttacttcc gggatagggc cccgcccgaa ccggggaaac cggccccgcc 60 tgctcagcaa cggctgcccc caacgccgac cccgccggcg gcccctcgca ggcctagccc 120 acccgcacca aggctccgcc cccggcagct gtggcggcac tgggctttaa ctttctcctt 180 cagcccgata gggacctagc ggggaaagcg ggcgtgacgt atagcaagtg gcggccagcc 240 cgcgtctggc gaattgacga gccgcacgag cagccgtgtg tcgagaaatt gcctatggtc 300 ccagagtcag aaagattttc atctatgacg atggtggact ttacaggagc acatacggac 360 cgtggagttt ttcgtgctgg ccagtggggg cgaaagacaa tcatcagcga gggcctgtac 420 cacggaacac aaataccatt catcaaaatg gcccttcaaa gacatggggg agtgattgta 480 ctgaatccca atgacaatct tgttgacctg aagactgaaa gggagaagct aagcttttct 540 accagagaag aatcaccaac ttctttgcac tcctgccagt cgatccccag gcagtgcaac 600 agtaaccctg aagaacatac catgtatgta tgggatcatt tcatttctaa aagtgcagcc 660 aagaatgtgg ctttcattgc ccatggctat ggtggtttgg tgttcactga tctgctcgtg 720 cagagaaaat gggaagtgat gaacaaagtg ttctctgtgg cattcatcga ttccatgcac 780 aatatacagc atcaggctag aaatgatcca caaatacaat ggtggataca gaaacactgc 840 cgtgaatggg tatcaaacaa taagccttta ggtaaagctg tgggctccct tgtcaaagtg 900 aattgtccaa ctgtctcagc tggaactgaa aagtatgcct tagcaccttc ctactgcttg 960 cattccatct ttaagtacct gaacagcacc ctgaaagcca agaccacaac agcctttacg 1020 cgttcaccaa ttgcaacccg aagcagcgaa agtaagaaag ctggtaaata aaggtgtact 1080 gacttgggtt tgatagtaat ctattctttt catagccatt cctgcagcaa agatccttca 1140 ctatggagag acttaaggtt ctaagtttga agacgctttg tttttgagaa acacaaaaat 1200 acaagttaac catgtgggg 1219  

Claims (7)

A testicular-specific nucleotide sequence comprising a nucleotide sequence selected from the group consisting of SEQ ID NOs: 1 to 110, a sequence complementary to the nucleotide sequence, or a fragment of the nucleotide. The testicular-specific nucleotide sequence of claim 1, wherein the testis is a testis of mammals or birds. 3. The testis-specific nucleotide sequence of claim 2, wherein the testis is a testis of algae. An avian testicular-specific nucleotide sequence comprising a nucleotide sequence selected from the group consisting of SEQ ID NOs: 1 to 110, a sequence complementary to the nucleotide sequence, or a fragment of the nucleotide. 5. The bird testicular-specific nucleotide sequence of claim 4, wherein said bird is chicken, quail, turkey, duck, goose, pheasant or pigeon. 3. The avian testis-specific nucleotide sequence of claim 2, wherein said alga is a chicken. Methods of identifying testicular cells comprising the following steps: (a) hybridizing a DNA obtained from a cell or a fragment thereof with the avian testicular-specific nucleotide sequence of any one of claims 1 to 6; And (b) confirming hybridization in step (a).

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