KR20050119800A - Xylaria sp. ah001 strain producing griseofulvin, formulation for controlling plant diseases containing same and method for controlling plant diseases using same - Google Patents

Abstract

The invention plant diseases using the antagonistic draw Seo pulbin (griseofulvin) or character Ilaria formulation, the microbial agent for controlling plant diseases comprising the (Xylaria) in AH001 strain having its producing ability has an activity for controlling plant diseases To a biological control method, a novel Xylaria genus AH001 strain that produces the griseofulvin or dichlorogriseofulvin, a method of producing griseofulvin or dichlorogriseofulvin from the strain In relation to the present invention, the griseofulvin and the genus AH001 strains having a production capacity thereof include rice blast, rice leaf blight, wheat red rust, barley flour, pulp wilting disease, pepper anthracnose or apple tree spot deciduous disease. It exhibits strong antagonism against plant diseases and can be usefully used for plant disease control.

Description

Xylaria genus AH001 strain that produces griofulvin, a plant disease control agent comprising the same and a method for controlling plant disease using the same XYLARIA SP. AH001 STRAIN PRODUCING GRISEOFULVIN, FORMULATION FOR CONTROLLING PLANT DISEASES CONTAINING SAME AND METHOD FOR CONTROLLING PLANT DISEASES USING SAME}

The present invention is a so Seo pulbin (griseofulvin) or chair having a counter-producing ability Ilaria (Xylaria) in plant disease using the agent, the agent for controlling plant diseases comprising the AH001 strain having antagonistic activity for controlling plant diseases how to control the biological and the draw Seo pulbin or dichloro draw novel character producing Seo pulbin (dichlorogriseofulvin) Ilaria (Xylaria) in AH001 strain and a method for so producing Seo pulbin or dichloro draw Seo pulbin from the strain will be.

Griseofulvin is an antibiotic with a representative antimicrobial activity produced by the fungus, and is particularly widely used against skin pathogens. In addition, griseofulvin is effective against veterinary medicine and plant diseases, and is known to be effective against plant diseases caused by Botrytis bacteria (Yamaguchi, Modern selective fungicides-properties, applications, mechanisms of action , pp. 415-429 (1995). Griseofulvin is produced by many species of the genus Penicillium , which causes severe stunting, excessive branching, abnormal swelling, and twisting of mycelia. twisting of hyphae). This antimicrobial substance inhibits the growth of many mycelial fungi, but it is known to be ineffective against bacteria belonging to yeast, actinomycetes and Oomycetes.

Griseofulvin can be prepared by chemical synthesis (Lednicer and Mitscher, 1977, The Organic Chemistry of Drug Synthesis, pp. 313-317), but there are so many intermediate reactions involved that it is not economically viable for commercial use. At present, the commercial production of Griseofulvin is carried out through fermentation with penicillium strains (Venkata Dasu and Panda, 1999, Bioprocess Engineering 21: 489-495). So, the chamber Penny bacteria are known to produce very Seo Seo pulbin Solarium pulbin (P. griseofulvin), Penny Room Solarium wave tyulryum (P. patulum), Penny Room Solarium yuti cache (P. urticae), Penny Room Solarium age so kanseu ( P. nigricans ), Penicillium sclerotigenum (Mantle et al., 1984, J. Gen. Microbiol. 130: 1293-1298; Yoshihiro et al., 1978, Tennen Yuki Kagobutsu Toronkai Koen Yoshishu 21: 152-158), Aspergillus versicolor (David and Paul, 1976, Phytochemistry 15: 1037-1039) and Streptomyces albolongus ( Terekhova et al., 1992, Antibiotiki I Khimioterapita 37: 19-21). Among them, griseofulvin was produced to be commercially economical through the development of large-scale production strains and fermentation process through mutations of the genus Penicillium. Therefore, it is very important commercially and academically to select new strains to produce glyseofulvin at low cost.

Therefore, the present inventors found that the culture medium of one strain has high antimicrobial activity against various phytopathogens while studying the new material produced by endogenous fungi isolated from plants, especially trees. The substance was found to be griseofulvin, and the strain producing it was found to be a strain of genus Xylaria , which has never been reported as a production strain of griseofulvin . In addition, the culture medium of this strain is plant diseases other than ash fungus caused by Botrytis cinerea , rice blast, rice leaf blight, wheat red rust, barley powdery mildew, pulp wilting disease, pepper anthrax or apple tree spots. It has been found that there are antagonisms against various plant diseases such as deciduous diseases.

It is an object of the present invention to provide an agent for controlling plant diseases, which comprises glyofulvin or a strain of genus Xylaria producing the same.

Another object of the present invention is to provide a method for controlling plant diseases using the agent.

It is another object of the present invention to provide a strain of genus Xylaria having gseleofulvin or dichlorogriseofulvin production capacity.

In order to achieve the above object, the present invention is for the control of plant diseases containing glyseofulvin of the formula (1), Xylaria genus AH001 strain (KCTC 10629BP) having a production capacity or a substance derived from the strain as an active ingredient Provide formulations.

In order to achieve the above another object, the present invention provides a method for controlling plant diseases using the agent.

In order to achieve the above another object, the present invention provides a method for producing glyopoolbin or dichloroglyopulbin of the formula (2) from the genus AH001 strain (KCTC 10629BP) of Xylaria.

The strain isolated in the present invention was isolated from the pine trunk, the morphological characteristics of the strain, 5.8S rRNA gene (ITS1-5.8S-ITS2) sequence analysis was confirmed as a strain of genus Xylaria, which is the genus Xylaria It was named AH001 and deposited on May 10, 2004 as a deposit number No. KCTC 10629BP to Gene Bank of Korea Research Institute of Bioscience and Biotechnology.

The Xylaria genus AH001 strain of the present invention has the following morphological and biochemical characteristics.

That is, AH001 strain of genus Xylaria produces a lot of yellow aerial hyphae on potato extract-dextrose agar medium, and the color of the back side of the medium becomes brown as time passes. In addition, long-term incubation in potato agar medium produces long vertical bat-shaped stromatas. Xylaria cornu-damae registered in GeneBank as a result of analyzing the nucleotide sequence (SEQ ID NO: 1) of the 5.8S rRNA gene (ITS1-5.8S-ITS2) for accurate identification of the strain ) And at least 90% similarity with AF163031.

Griseofulvin and dichlorogriseofulvin can be produced from the Xylaria genus AH001 strain having these characteristics. The cultures of the strains include potato extract-dextrose broth (PD), malt extract-peptone-dextrose. For the cultivation of fungi such as malt extract peptone dextrose broth (MPD), malt and yeast extract broth (MY), glucose-yeast extract-polypeptone liquid medium (YpSs broth (YpSs) Conventional media used can be used, with glucose-yeast extract-polypeptone media particularly preferred. A large amount of griseofulvin and dichlorogriseofulvin can be produced by inoculating a suitable strain of the genus AH001 in these medium and incubating for 3 to 21 days, preferably 12 to 18 days.

The Xylaria genus AH001 strain, and the griseofulvin isolated therefrom have a strong antagonistic action against rice blast, rice leaf blight, wheat red rust, barley powdery mildew, fusidium wilting disease, chili anthracnose or apple tree spotted deciduous disease. Indicates.

Xylaria genus AH001 strain of the present invention can be used as an agent for controlling plant diseases by formulating into a powder, pellets, granules or solutions by mixing the carrier itself, or the culture solution, extract or spores of the strain alone. As the carrier, water, white carbon, kaolin, or the like can be used.

Formulated formulations can control the soil on which plants are growing or the surface of growing plants to control inhibition of plant growth by plant diseases and thereby death.

Xylaria AH001 strain of the present invention can be treated in an amount of 10 ⁵ cells / ml to 10 ¹¹ cells / ml, preferably 10 ⁶ cells / ml to 10 ⁸ cells / ml to the target plant, Griseofulvin isolated from the strain of Illaria genus AH001 can be treated in an amount of 100 μg / ml to 1000 μg / ml, preferably 250 μg / ml to 500 μg / ml for the subject plant.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited thereto.

Example 1 Isolation of Xylaria AH001 Strains

Healthy branches and stems are collected from Abies holophyiia native to Korea, cut into 1 cm each, and then immersed in 96% ethanol for 1 minute and 3.25% sodium chloride (NaOCl) for 3 minutes. Surface sterilization with 96% ethanol for 30 seconds. The first surface sterilized sample was flame sterilized again using an alcohol lamp, and the sample was then treated with cornmeal malt extract-yeast extract agar medium to which 50 mg / L chloramphenicol (available from Sigma, USA) was added. medium: 17.0 g corn flour, 20.0 g malt extract, 2.0 g yeast extract, 1.0 L distilled water) and the bacteria were separated under 25 ℃ constant temperature conditions. The isolates were isolated from single mycelia on a potato agar medium (Becton and Dickinson, USA) to isolate a total of nine fungi. For the long-term storage of these strains, seven fungi were isolated on potato agar medium. The tip of the cultured strain was removed and placed in sterile distilled water and then stored at room temperature, or further placed in dimethylsulfoxide (dimethylsulfoxide, DMSO) solution and then stored at -70 ° C.

Example 2: In vivo of isolated strains in vivo Antimicrobial Activity

In order to investigate the control activity against the six plant diseases of the nine strains isolated in Example 1 was carried out liquid culture as follows. The tip of the strain cultured in potato extract-dextrose agar medium in sterile 200 ml potato extract-dextrose broth (PDB), obtained from Becton and Dickinson, USA 8 mm in diameter), and then inoculated into 4 pieces and shaken at 25 ° C. and 150 rpm for 10 days. The culture solution was centrifuged at 10,000 x g for 15 minutes, and a sample in which Tween 20 was added at a level of 250 µg / ml to 30 ml of supernatant was used as a sample.

In order to select strains with antimicrobial activity, the antimicrobial activity of the nine strain culture samples was assayed against plant diseases of rice blast, rice leaf blight, tomato gray mold, tomato blight, wheat rust and barley powdery mildew. It was. Rice ( Oryza sativa ), tomato ( Lycopersicon esculentum ), barley ( Hordeum sativum ) and wheat ( Triticum aestivum ) in 4.5 cm diameter plastic pots contain about 70% of horticultural or tap water soils in a greenhouse (25 ± 5 ℃). I grew about three weeks in a week.

Rice blast is a two-leafed seedling with Magnaporthe grisea (obtained from Korea Research Institute of Chemical Technology). After inoculation by spraying a spore suspension (5 × 10 ⁵ spores / ml), the disease was induced for one day in a 25 ° C. wet room, and then placed in a 25 ° C. thermostat for 5 days. Rice leaf blight blight was inoculated with 3 leaf seedlings inoculated with medium ( Branberry 90 g, rice husk 15 g, distilled water 100 ml) incubated for 7 days with three leafy seedlings of Thanatephorus cucumeris ( source : Korea Research Institute of Chemical Technology). After 4 days of treatment in a wet room, it was incubated for 4 days in a 25 ℃ constant temperature room. Tomato late blight was detected in two-stage tomato seedlings of Phytophthora infestans (Gangneung National University). After inoculation of the distilled suspension, which was extracted from the distilled sac suspension (10 ⁵ sachets / ml), the onset was induced in a 20 ° C wet room.

On the other hand, tomato gray mold disease was developed in a wet room after treatment of the spore suspension (10 ⁶ spores / ml) of tomato fungi ( Botrytis cinerea ; obtained from Korea Research Institute of Chemistry) on tomato seedlings. Wheat rust is sprayed with spore suspension after suspending spores of Puccinia recondita ( Puccinia recondita ; source: Korea Research Institute of Chemistry), a twenty seedling seedling, at a level of 0.67 g spores / L in Tween 20 solution (250 ㎍ / ml). After treatment, the disease was induced in a 20 ° C. wet room for one day, and then transferred to a constant temperature room and cultured. Lastly, barley powdery mildew was inoculated with spores of Blumeria graminis f. Sp.hordei (obtained from Korea Research Institute of Chemical Technology) that were passaged in host plants at the first stage of barley seedlings. As a control, 30 ml of Tween 20 solution (250 µg / ml) mixed with 1% DMSO was used.

After 7 days for rice blast, wheat rust and wheat flour, 8 days for rice leaf blight, and 3 days and 4 days for tomato gray mold and tomato late blight, the disease area ratios were examined. Among the nine isolates, the strain named AH001 showed the strongest antimicrobial activity, and the results are shown in Table 1 below.

Plant disease Rice Blast Rice leaf blight Tomato gray mold Tomato plague Wheat Red Rust Wheat flour Control Price (%) 99 90 97 10 99 96 Control Value (%) = (Untreated Control Incidence-Treated Group Incidence) ÷ Untreated Control Incidence

As shown in Table 1, the AH001 strain of the genus Xylaria against the other five plant diseases except rice blight, rice leaf blight, tomato ash mold, wheat red rust and wheat powdery mildew among six plant diseases High control activity was shown.

Example 3: Identification of AH001 Strains of Genus Xylaria

The identification of the AH001 strain among the nine strains isolated in Example 1 was performed using various morphological characterization and sequencing of the 5.8S rRNA gene (ITS1-5.8S-ITS2).

(1) Cultural and Morphological Characteristics

AH001 strains cultured in potato agar medium had yellow mycelia (FIG. 1) and the color of the back side of the medium turned brown over time. After the incubation time, a vertical bat-shaped stromata was formed on the cells.

(2) nucleotide sequence analysis of 5.8S rRNA gene (ITS1-5.8S-ITS2)

For accurate identification of the strain, the AH001 strain was inoculated on a potato agar medium coated with cellophane paper, incubated at 25 ° C. for 4 days, and DNA was extracted from the mycelium. From ITS4 and 5'-primers (White et al., 1990, PCR protocols: a guide to methods and application, pp.315-322) from extracted DNA, ITS1-5.8S- of nuclear ribosomal DNA (nuclear rDNA) After amplification of ITS2, the purity was checked, and the nucleotide sequence was determined by using an ABI3700 automated DNA sequencer (Applied Biosystems, Inc.). Nucleotide sequence of ITS1-5.8S-ITS2 (SEQ ID NO: 1) showed more than 90% similarity with GeneBank's Xylaria cornu-damae AF163031 and was identified as a genus of Xylaria . Figure 2 shows the phylogenetic lineage of Xylaria AH001 strain through analysis of the nuclear ribosomal ITS1-5.8S-ITS2 sequence of the genus Xylaria AH001.

As identified above, the genus Xylaria strain AH001 was deposited with KCTC 10629BP dated May 10, 2004 to the Korea Biotechnology Research Institute Gene Bank.

Example 4 Isolation and Purification of Antimicrobial Substances

The tip portions of the strains grown on potato agar medium in 14 L potato juice medium were removed with a cork cutter (8 mm in diameter), and then inoculated four per 200 ml of potato juice medium and incubated at 25 ° C. and 150 rpm for 10 days. The culture solution was centrifuged at 10,000 × g for 15 minutes, and only the supernatant was extracted with twice the same amount of ethyl acetate, and then concentrated under reduced pressure at 40 ° C. Investigation of the antimicrobial activity of the water layer and ethyl acetate extracts against the six plant diseases showed that the ethyl acetate extract showed antimicrobial activity. Thus, in order to separate the active substance from the ethyl acetate extract, 10 ml of ethyl acetate extract was equilibrated with a solvent [chloroform: methanol, 95: 5 (v: v)] to silica gel (70-230 mesh, 200 g; TLC (solvent: chloroform: methanol solvent (95: 5, 9: 1 and 10: 1, v: v)) was performed using a column packed with Merck, Germany (3.6 × 60 cm). As a result, three fractions F1, F2 and F3 were obtained, and the fractions showed activity in the F2 (600 mg) fraction as a result of examining the antimicrobial activity in vivo at the concentration of 500 µg / ml. In order to confirm that the material of the F2 fraction was a single substance, an analysis sample was added dropwise to TLC (20 × 20 cm, 0.25 mm thickness, Kiesel gel 60 F254; Merck). As a result, when TLC analysis was performed using a chloroform: methanol (9: 1, v: v) solvent, it appeared as a single substance, while hexane: ethyl acetate: methanol (60: 40: 1, v: v: v When TLC analysis was performed with the solvent ratio, two substances appeared. Thus, the F2 (600 mg) fraction was collected in a preparative TLC plate (20 × 20 cm, thickness 0.5 mm, Kiesel gel 60 F254 obtained from Merck) and hexane: ethyl acetate: methanol (50: 50: 2, v: v: v) The two materials were purely separated using a solvent. As a result, 40 mg and 350 mg of nonpolar and polar substances were separated purely.

Example 5 Structure Determination of Griseofulvin and Dichlorogriseofulvin

The two active materials separated and purified in Example 4 were subjected to mass spectrometry using a mass spectrometer (JEOL JMS-DX303; obtained from Nippon Electronics Co., Ltd.). As a result, the molecular weights of the two substances were 352 Da (molecular formula C ₁₇ H _17). ClO ₆ ) and 318 Da (molecular formula C ₁₇ H ₁₈ O ₆ ), which were analyzed by electron impact mass spectroscopy (EI-MS) and the results are shown in FIGS. 3 and 4, respectively. It was.

In addition, to determine the structure of the two active substances, each 20 mg was completely dried, dissolved in 0.5 ml of CDCl _{3 and placed} in a 5 mm NMR tube and subjected to NMR analysis (Bruker-AMX 500 (500 MHz)), ¹ H-NMR was measured at 500 MHz and ¹³ C-NMR at 125 MHz. Table 1 shows the results of connecting carbon and hydrogen through the ¹ H- ¹³ C COZY spectrum and connecting the carbon skeleton through the ¹ H- ¹ H COZY spectrum.

Accordingly, the two substances were determined to be griseofulvin and dichlorogriseofulvin, respectively, and the structural formulas of the two substances are represented by Chemical Formulas 1 and 2, respectively.

Formula 1

Formula 2

Example 6 In Vitro of Griseofulvin and Dichlorogriseofulvin in vitro ) Antibacterial activity verification

In vitro for the plant diseases of griseofulvin and dichlorogriseofulvin identified in Example 5 above To measure the antimicrobial activity, Magnaporthe grisea (obtained from Korea Research Institute of Chemical Technology) on a solid medium containing two antimicrobial substances, Fusarium oxysporum (obtained from Korea Research Institute of Chemical Technology) , phytophthora capsici (obtained from Chungnam National University), Colletotrichum gloeosporioides (obtained from Korea Research Institute of Chemical Technology), Phytophthora infestans (obtained from Kangnung National University), apples, Alternaria mali (obtained from Kyung-Nong), Botrytis cinerea (from Korea Chemical Research Institute) and rice leaf blight The mycelial growth of eight plant pathogens of Corticium sasaki (Korea Chemical Research Institute) was investigated. Tomato blight and pepper blight were used for V-8 juice agar, and the other six phytopathogens were potato agar. After sterilization of each antimicrobial medium, the antimicrobial substance dissolved in DMSO before solidification was added to the medium so that the final concentration was 200, 66.7, 22.2, 7.4, 2.5 and 0.82 μg / ml. DMSO 1% was added to the untreated group. After killing the mycelia of each bacterium in the middle of the medium, tomato late blight and tomato gray fungus were incubated at 20 ° C, and the rest of the bacteria were incubated at 25 ° C. After incubation for 2 to 6 days, the mycelial growth was measured to determine 50% mycelial growth inhibition concentration, and the results are shown in Table 3.

Plant pathogen 50% mycelial growth concentration (㎍ / ㎖) Griseofulvin Dichlorogriseofulvin Rice Bacteria 1.7 ＞ 200 Futurium wilt 30 ＞ 200 Chilli Plague ＞ 200 ＞ 200 Tomato blight ＞ 200 ＞ 200 Pepper anthrax 1.7 ＞ 200 Apple spotted deciduous bacterium 18 ＞ 200 Tomato gray mold 5.0 ＞ 200 Rice leaf blight 11 ＞ 200

As shown in Table 3, griseofulvin was shown to have a much higher antibacterial activity than dichlorogriseofulvin. In the case of griseofulvin, phytopathogenic fungi belonging to Oomycetes, such as tomato and fungal bacterium, showed little antimicrobial activity while high antibacterial activity against other fungi. In particular, 50% mycelial growth inhibitory concentration was 1.7 ㎍ / ml for Colletotrichum gloeosporioides and Magnaporthe grisea , and 5.0 ㎍ / ml for Botrytis cinerea .

Example 7 In Vivo of Griseofulvin and Dichlorogriseofulvin in vivo Antibacterial activity

The antimicrobial activity of griseofulvin and dichlorogriseofulvin against plant diseases was observed in the same manner as in Example 2, and the results are shown in Table 4.

As shown in Table 4, the treatment of dichlorogriseofulvin at 150 ㎍ / ㎖ concentration showed 70% against rice leaf blight blight, 93% against barley powdery mildew, low concentration 50 ㎍ / ㎖ The dichloroglyopulbin treatment of showed a generally low activity of less than 50%. On the other hand, when treated with 150 ㎍ / ㎖, griseofulvin showed a high control effect of more than 60% for all plant diseases except tomato late blight, and even when treated at a low concentration (50 ㎍ / ㎖) Indicated. In particular, it showed very high activity against rice blast, wheat red rust, barley flour and rice leaf blight.

Example 8 Production of Active Substances of AH001 Strains of Genus Xylaria According to Medium and Culture Period

In order to determine the maximum production conditions of two antimicrobial substances produced by Xylaria AH001 strains, the yields were investigated by varying the media type and culture period. The medium includes potato juice medium, malt extract-peptone-dextrose medium (MPD; 20 g malt extract, 20 g dextrose, 1 g peptone, 1.0 L distilled water), malt and yeast extract medium (MY; 3 g malt extract, 0.5 g KH ₂ PO _4, 2 g yeast extract, 0.5 g MgSO ₄ · 7H ₂ O, 1.0 L distilled water) and glucose-yeast extract-polypeptone medium (YpSs; 20 g glucose, 2 g yeast extract, 5 g polypeptone , 0.5 g MgSO ₄ , 1 g KH ₂ PO ₄ , 1.0 L distilled water) was used. 200 ml of each medium was placed in a 500 ml Erlenmeyer flask, and after sterilization, the tip of the strain cultured in potato agar medium was removed with a cork cutter (8 mm in diameter) to inoculate four pieces. 2 ml of the culture solution on days 3, 6, 9, 12, 15, 18 and 21 were transferred to each vial with shaking culture at 25 ° C. and 150 rpm. Extracted twice with the same amount of ethyl ether, dried with nitrogen gas, redissolved with 0.5 ml of ethyl acetate, and then selectively ionized using a gas chromatograph-mass spectrometer. Quantitative analysis was performed in a selected ion monitoring (SIM) mode. The column was Hicap CBP20-M25-025 capillary column, the injector temperature and the interface temperature was adjusted to 280 ℃ and 250 ℃, respectively. The column temperature was continued for 15 minutes at the initial 200 ℃, after raising the temperature to 8 ℃ per minute and lasted for 15 minutes at the final temperature of 280 ℃. The target ion of griseofulvin was set to m / z 352, and the target ion of dichlorogriseofulvin was set to m / z 318.

As a result, the highest amounts of dichlorogriseofulvin and griseofulvin were produced in the YpSs medium among the four media used. Figure 5 shows the amount of production according to the incubation time in four medium of the griseofulvin showed the maximum production amount (800 ㎍ / ㎖) at 15 days of culture. It can be seen that the production starts from the 6th day of culture and on the 9th day, a large amount of metabolites are produced rapidly. On the other hand, a small amount of active material was also produced in MPD medium and potato juice medium, unlike the YpSs medium, the production was continuously increased even after 21 days of incubation period. Dichlorogriseofulvin was also produced in a very similar fashion and showed the maximum production (579 μg / ml) at 15 days of culture in YpSs medium.

As described above, the griseofulvin and the Xylaria AH001 strain having its production capacity are plant diseases such as rice blast, rice leaf blight, wheat red rust, barley flour, pulp wilting disease, pepper anthrax, or apple tree spot deciduous disease. It exhibits strong antagonistic activity against and can be usefully used for controlling plant diseases.

1 is a photograph of AH001 strain of genus Xylaria cultured in potato extract-dextrose agar medium.

Figure 2 shows the phylogenetic lineage of Xylaria AH001 strain through analysis of the nucleotide sequence of nuclear ribosomal 5.8S rRNA gene of the genus Xylaria AH001.

Figure 3 is an electron collision mass spectrum analysis result of the griseofulvin isolated from Xylaria AH001 strain.

Figure 4 is an electron collision mass spectrum analysis of dichlorogriseofulvin isolated from Xylaria AH001 strain.

Figure 5 is a graph showing the degree of production of griseopulin according to the medium from the genus Xylaria AH001.

<110> KOREA RESEARCH INSTITUTE OF CHEMICAL TECHNOLOGY <120> XYLARIA SP. AH001 STRAIN PRODUCING GRISEOFULVIN, FORMULATION FOR CONTROLLING PLANT DISEASES CONTAINING SAME AND METHOD FOR CONTROLLING PLANT DISEASES USING SAME <130> FPD / 200405-0020 <160> 1 <170> KopatentIn 1.71 <210> 1 <211> 643 <212> DNA <213> XYLARIA SP. AH001 ITS1-5.8S-ITS2 <400> 1 gttcctattg agcagagtac tactccaacc catgtgacat acctattgtt gcctcggcag 60 gtcgtagact gctgggaaag ggccttcccc tagcttggga ggccctaccc gggagccgcg 120 ggtaagcctg ccgatggccc gcaaaaactc tgtttagcac tgaaattctg aatcataact 180 aaataagtta aaactttcaa caacggatct cttggttctg gcatcgatga agaacgcagc 240 gaaatgcgat aagtaatgtg aattgcagaa ttcagtgaat catcgaatct ttgaacgcac 300 attgcgccca ttagtattct agtgggcatg cctgttcgag cgtcatttca acccttaagc 360 ccttgttgct tagtgttggg agactacgtt ccttagcgta gctcctcaaa gttagtggca 420 gagtcagttc aaactctagg tgtagtaaat ttcttctcgc ctctgtggtt gggctgcccc 480 tcgccgtaaa acacccctaa tttttaaagg ttgacctcgg atcaggtagg aatacccgct 540 gaacttaagc atatcaataa ggcggaggaa aagaaccctc tgggtttgct ctactcaaga 600 cgggtgaatt gggtatagct ttatgcgggg aataatggca gga 643

Claims (9)

Griseofulvin, Xylaria genus AH001 (KCTC 10629BP) strain having its production capacity or a plant-derived agent containing a substance derived from the strain as an active ingredient. The method of claim 1, Formulation according to claim Seo draw the full bin chair Ilaria (Xylaria) in AH001 (KCTC 10629BP) that produced from the strain. The method of claim 1, Formulation characterized in that the material derived from the strain Xylaria AH001 is a culture, extract or spores of the strain. The method of claim 1, An agent characterized in that the plant disease is rice blast, rice leaf blight, wheat red rust, wheat powdery mildew, fusidium wilting disease, pepper anthrax or apple tree spotted deciduous disease. A method of controlling plant diseases using the agent of any one of claims 1 to 4. The method of claim 5, The plant disease is rice blast, rice leaf blight, wheat red rust, wheat powdery mildew, fusidium wilting disease, pepper anthrax or apple tree spotted deciduous disease. AH001 (KCTC 10629BP) strain of the genus Xylaria , which has the ability to produce griseofulvin or dichlorogriseofulvin. Here Ilaria (Xylaria) in AH001 (KCTC 10629BP) or draw Seo pulbin dichloro method of production so Seo pulbin which comprises culturing the strain. The method of claim 8, Xylaria AH001 strains were prepared from potato extract-dextrose broth (PD), malt extract-peptone-dextrose broth (MPD), malt extract-yeast extract liquid medium (malt). and yeast extract broth; MY) or glucose-yeast extract-polypeptone liquid medium (YpSs broth; YpSs) for 3 to 21 days.