KR20050086968A - Dna chips for detection of peptostreptococcus anarobius bacilli - Google Patents

Dna chips for detection of peptostreptococcus anarobius bacilli Download PDF

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KR20050086968A
KR20050086968A KR1020057014603A KR20057014603A KR20050086968A KR 20050086968 A KR20050086968 A KR 20050086968A KR 1020057014603 A KR1020057014603 A KR 1020057014603A KR 20057014603 A KR20057014603 A KR 20057014603A KR 20050086968 A KR20050086968 A KR 20050086968A
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nucleic acid
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유승민
유소영
금기창
유원민
유내춘
이상엽
장경희
이진
김준명
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(주)메디제네스
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Abstract

본 발명은 생물학적 시료중에서 비바이러스 감염 유기체를 검출 및 동정하는데 유용하고 그러한 비바이러스 감염 유기체에 의해 발생되는 질환을 진단하는데 사용하는 핵산 분자 및 이러한 핵산 분자를 포함하는 핵산 탐침이 고정된 감염질환을 진단하는데 유용한 DNA 칩에 관한 것이다.The present invention is useful for detecting and identifying non-viral infectious organisms in biological samples and for diagnosing infectious diseases in which nucleic acid molecules and nucleic acid probes comprising such nucleic acid molecules are immobilized for use in diagnosing diseases caused by such non-viral infectious organisms. It is about a DNA chip useful for.

Description

펩토스트렙토코커스 언애어로비우스 균의 검출용 ＤＮＡ 칩{DNA chips for Detection of Peptostreptococcus anarobius bacilli}DNA chips for detection of Peptostreptococcus frozen Aerobic bacteria

본 발명은 생물학적 시료중에서 비바이러스 감염 유기체를 검출 및 동정하는데 유용하고 그러한 비바이러스 감염 유기체에 의해 발생되는 질환을 진단하는데 사용하는 핵산 탐침에 관한 것이다. 구체적으로, 본 발명은 비바이러스 감염 유기체의 rRNA 유전자로부터 유도되어 그들 유기체를 검출 및 동정하는데 유용한 핵산 탐침 및 이 핵산 탐침을 포함하여 감염질환을 진단하는데 유용한 조성물 및 키트에 관한 것이다.The present invention relates to nucleic acid probes useful for detecting and identifying nonviral infectious organisms in biological samples and for use in diagnosing diseases caused by such nonviral infectious organisms. In particular, the present invention relates to nucleic acid probes derived from the rRNA genes of nonviral infectious organisms and useful for detecting and identifying those organisms, and compositions and kits useful for diagnosing infectious diseases, including these nucleic acid probes.

(배경기술)(Background)

감염질환은 병원체가 혈액, 체액 및 조직 내에 출현하여 서식하기 시작하면서 발병하는 질환으로서, 원인균의 정확한 동정 및 적절한 치료가 이루어지지 않을 경우, 생명을 잃을 수 있는 질병이다. 더욱이 최근에는 항생제투여의 오남용으로 인해 병원체의 배양률이 감소하게 되었고, 이식에 따른 면역억제제 사용의 증가, 항암 치료로 인한 약물투여의 증가 등으로 인한 원인병원체가 다양해지게 되면서 배양 검사와 같은 기존의 감염질환을 진단하는 진단방법들이 점차 어려움에 부딪히고 있는 실정이다. 특히 혐기성 균을 포함한 특정 병원체의 경우 인체에 매우 치명적이고 중한 질환을 야기시키기 때문에 이를 조속히 진단함과 동시에 원인 병원체의 종류를 확인하는 것이 치료에 있어서 상당히 중요한 위치를 차지한다. 이와 같은 필요성으로 인해 오랫동안 감염질환 원인 미생물을 동정하고자 하는 진단 방법들이 연구 및 개발되어 왔다. 지난 10년 동안에 많은 미생물을 검출하는데 상당한 진보가 있어 왔지만 현재 이용되고 있는 진단 방법들은 여전히 많은 노동을 필요로 하고 있고 민감도 및 특이성이 낮은 형편이다.Infectious diseases are diseases that occur when pathogens start to live in the blood, body fluids, and tissues, and may be life-threatening if accurate identification and proper treatment of causative organisms are not performed. More recently, the misuse of antibiotics has resulted in a reduction in the incidence of pathogens, and as the causative pathogens have been diversified due to the increased use of immunosuppressants following transplantation and the increase in drug administration due to anticancer treatments, such as culture tests. Diagnostic methods for diagnosing infectious diseases are increasingly difficult. In particular, in the case of certain pathogens, including anaerobic bacteria, it causes a very fatal and serious disease in the human body, promptly diagnosing them and identifying the type of the pathogens are very important in the treatment. Due to this necessity, diagnostic methods for identifying microorganisms causing infectious diseases have been researched and developed for a long time. Significant advances have been made in detecting a large number of microorganisms over the past decade, but the diagnostic methods currently in use still require a lot of labor and are low in sensitivity and specificity.

바이러스를 제외하고, 모든 원핵 유기체는 원핵성 5S, 16S 및 23S rRNA 분자들의 상동체를 코딩하는 rRNA 유전자를 함유한다. 진핵 세포의 경우는 이들 rRNA 분자들이 원핵 세포의 분자와 실질적으로 유사한 5S rRNA, 5.8S rRNA, 18S rRNA 및 28S rRNA이다. 생물학적 시료에서 특정한 유기체 또는 유기체 군의 rRNA 아서열을 특정한 표적으로 하여 검출하기 위한 탐침들이 많이 공개되어 있다. 이러한 핵산 탐침을 잘 알려진 중합효소 연쇄 반응(PCR)과 함께 병용함으로써 종래의 진단 방법에서의 상기 문제점가운데 많은 것들이 해결될 수 있었다. 진단을 위한 핵산 탐침 기술에서는 증폭시키는 표적 유전자의 선택이 매우 중요한데 대체로 rRNA, 특히 23S rRNA 유전자 및 내부전사지역 (Internal Transcribed Spacer Region, ITS)이 이용되고 있으며 이들에 대한 핵산 탐침서열은 유리하게는 적당한 긴축 조건하에서 다른 유기체로부터 유래된 핵산과 교차 반응하는 확률이 낮은 것으로 알려져 있다 (P. Wattiau et. al., Appl. Microbiol. Biotechnol., 56, 816-819, 2001; D, A. Stahlm et. al., J. Bacteriol., 172, 116-124, 1990; Boddinghaus. et. al., J. Clin., Microbiol., 28, 1751-1759, 1990; T. Rogall et al., J. Gen. Microbiol., 136, 1915-1920, 1990; T. Rogall, et. al., Int. J. System. Bacteriol., 40, 323-330, 1990; K. Rantakokko-Jalava et. al., J. Clin., Mirobiol., 38(1), 32-39, 2000 ; Park et. al., J. Clin., Mirobiol., 38(11), 4080-4085, 2000; A. Schmalenberger et. al, Appl. Microbiol. Biotechnol., 67(8), 3557-3563, 2001; 국제특허공개 WO98/55646; Jannes 등의 미국특허 제6025132호; 및 Rossau 등의 미국특허 제6,277,577호).Except for viruses, all prokaryotic organisms contain rRNA genes that encode homologs of prokaryotic 5S, 16S and 23S rRNA molecules. For eukaryotic cells, these rRNA molecules are 5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA, which are substantially similar to those of prokaryotic cells. Many probes have been published for the specific targeting of rRNA subsequences of specific organisms or groups of organisms in biological samples. By using these nucleic acid probes in combination with well known polymerase chain reaction (PCR), many of these problems can be solved in conventional diagnostic methods. In nucleic acid probe technology for diagnosis, selection of target genes to be amplified is very important. In general, rRNA, in particular 23S rRNA gene and Internal Transcribed Spacer Region (ITS), are used, and nucleic acid probe sequences for these are advantageously suitable. It is known that the probability of cross-reaction with nucleic acids from other organisms under tightening conditions is low (P. Wattiau et. Al., Appl. Microbiol. Biotechnol., 56, 816-819, 2001; D, A. Stahlm et. al., J. Bacteriol., 172, 116-124, 1990; Boddinghaus. et.al., J. Clin., Microbiol., 28, 1751-1759, 1990; T. Rogall et al., J. Gen. Microbiol., 136, 1915-1920, 1990; T. Rogall, et.al., Int. J. System.Bacteriol., 40, 323-330, 1990; K. Rantakokko-Jalava et.al., J. Clin , Mirobiol., 38 (1), 32-39, 2000; Park et.al., J. Clin., Mirobiol., 38 (11), 4080-4085, 2000; A. Schmalenberger et.al, Appl. Microbiol.Biotechnol., 67 (8), 3557-3563, 2001; International Patent Publication WO98 / 55646, US Pat. No. 6025132 to Jannes et al., And US Pat. No. 6,277,577 to Rossau et al.).

그러나, 많은 병원체에 대하여 rRNA 유전자의 염기 서열이 동정되지 않은 상태에 있으며 그럼으로써 그러한 병원체의 rRNA 유전자의 염기 서열을 동정하고 그로부터 유도되는 고도의 특이성을 갖는 탐침을 개발할 필요가 여전히 남아있다. 또한, 비록 일부 병원체의 경우 rRNA 유전자의 염기 서열이 전체적으로 또는 부분적으로 밝혀져 있을지라도, 그러한 병원체를 보다 특이적으로 검출할 수 있는 새로운 핵산 탐침은 계속 개발될 필요가 있다.However, for many pathogens, the nucleotide sequence of the rRNA gene is in an unidentified state, and thus there remains a need to develop a probe with a high specificity to identify and derive the nucleotide sequence of the rRNA gene of such pathogen. In addition, although for some pathogens the nucleotide sequence of the rRNA gene is revealed in whole or in part, new nucleic acid probes capable of detecting such pathogens more specifically need to be developed.

이에, 본 발명의 목적은 하기의 비바이러스 병원체를 검출 및 동정하는데 유용한 핵산 탐침을 개발하는데 있다:It is therefore an object of the present invention to develop nucleic acid probes useful for detecting and identifying the following nonviral pathogens:

(1) 아시네토박터 바우마니 (Acinetobacter baumanii);(1) Acinetobacter baumanii;

(2) 언에어로비오스피리룸 숙시니시프로더센스(2) Unaerobiospyroom Succinsi Proder Sense

(Anaerobiospirillum succiniciproducens);Anaerobiospirillum succiniciproducens;

(3) 박테로이데스 프라질리스 (Bacteroides fragilis);(3) Bacteroides fragilis;

(4) 카디오박테리움 호미니스 (Cardiobacterium hominis);(4) Cardiobacterium hominis;

(5) 크리서박테리움 메닌고셉티쿰 (Chryseobacterium meningosepticum);(5) Chryseobacterium meningosepticum;

(6) 클로스트리디움 람모섬 (Clostridium ramosum);(6) Clostridium ramosum;

(7) 코마모나스 아시도보란스 (Comamonas acidovorans);(7) Comamonas acidovorans;

(8) 코리네박테리움 디프테리애 (Corynebacterium diphtheriae);(8) Corynebacterium diphtheriae;

(9) 크레브시엘라 옥시토카 (Klebsiella oxytoca);(9) Klebsiella oxytoca;

(10) 오크로박트룸 안트로피 (Ochrobactrum anthropi);(10) Ochrobactrum anthropi;

(11) 펩토스트렙토코코스 프레보티 (Peptostreptococcus prevotii);(11) Peptostreptococcus prevotii;

(12) 포피로모나스 진지발리스 (Porphyromonas gingivalis);(12) Porphyromonas gingivalis;

(13) 펩토스트렙토코커스 언애어로비우스 (Peptostreptococcus anarobius);(13) Peptostreptococcus anaeroius;

(14) 펩토스트렙토코커스 마그누스 (Peptostreptococcus magnus);(14) Peptostreptococcus magnus;

(15) 푸소박테리움 네크로포룸 (Fusobacterium necrophorum);(15) Fusobacterium necrophorum;

(16) 프로테우스 불가리스 (Proteus vulgaris);(16) Proteus vulgaris;

(17) 엔테로박터 에어로게네스 (Enterobacter aerogenes);(17) Enterobacter aerogenes;

(18) 스트렙토코커스 뮤탄스 (Streptococcus mutans);(18) Streptococcus mutans;

(19) 킨젤라 킨갭 (Kingella kingaep);(19) Kingella kingaep;

(20) 박테로이데스 오바투스 (Bacteroides ovatus);(20) Bacteroides ovatus;

(21) 박테로이데스 테타이오타오미크론 (Bacteroides thetaiotaomicron);(21) Bacteroides thetaiotaomicron;

(22) 클로스트리디움 디프실 (Clostridium diffcile);(22) Clostridium diffcile;

(23) 해모필러스 아프로필라스 (Haemophilus aprophilas);(23) Haemophilus aprophilas;

(24) 나이세리아 고노헤아 (Neisseria gonorrhea);(24) Neisseria gonorrhea;

(25) 아이케넬라 코로덴스 (Eikenella corrodens);(25) Eikenella corrodens;

(26) 박테로이데스 불가투스 (Bacteroides vulgatus);(26) Bacteroides vulgatus;

(27) 브란하멜라 카타르할리스 (Branhamella catarrhalis);(27) Branhamella catarrhalis;

(28) 수테렐라 와즈워텐시스 (Sutterella wadsworthensis);(28) Sutterella wadsworthensis;

(29) 액티노마이세스 이스라엘이 (Actinomyces israelii);(29) Actinomyces israelii;

(30) 스타필로코코스 에피더미디스 (Staphylococcus epidrmidis);(30) Staphylococcus epidrmidis;

(31) 벌코데리아 세파시아 (Burkholderia cepacia);(31) Burkholderia cepacia;

(32) 살모넬라 엔테리티디스 (Salmonella spp.(enteritidis));(32) Salmonella enteritidis (Salmonella spp. (Enteritidis));

(33) 에스케리치아 콜라이 (Escherichia coli);(33) Escherichia coli;

(34) 크레브시엘라 뉴모니에 (Klebsiella pneumoniae);(34) Klebsiella pneumoniae;

(35) 프로테우스 미라빌리스 (Proteus mirabilis);(35) Proteus mirabilis;

(36) 스트렙토코코스 뉴모니에 (Streptococcus pneumoniae);(36) Streptococcus pneumoniae;

(37) 비브리오 벌른피커스 (Vibrio vulnificus);(37) Vibrio vulnificus;

(38) 슈도모나스 애루지노사 (Pseudomonas aeruginosa);(38) Pseudomonas aeruginosa;

(39) 에로모나스 하이드로필라 (Aeromonas hydrophila);(39) Aeromonas hydrophila;

(40) 리스테리아 모노사이토제네스 (Listeria monocytogenes);(40) Listeria monocytogenes;

(41) 엔테로코코스 페슘 (Enterococcus faecium);(41) Enterococcus faecium;

(42) 스타필로코코스 아우레우스 (Staphylococcus aureus);(42) Staphylococcus aureus;

(43) 나이세리아 메닌자이티디스 (Neisseria meningitidis);(43) Neisseria meningitidis;

(44) 레지오넬라 뉴모필라 (Legionella pneumophila);(44) Legionella pneumophila;

(45) 캔디다 알비칸스 (Candida albicans); 및(45) Candida albicans; And

(46) 캔디다 글라브라타 (Candida glabrata).(46) Candida glabrata.

본 발명자들은 상기 각 병원체의 rRNA 유전자와 특정적으로 혼성화하고 다른 유기체의 핵산과는 교차 반응하지 않는 염기 서열을 갖는 탐침을 개발하였고 또한 이들 탐침을 집적한 DNA 칩을 제작하여 임상 실험을 통해 그들 탐침의 각 특이성을 확인함으로써 상기 본 발명의 목적을 달성하였다. 상기 (1) 내지 (28)의 각 세균의 검출 및 동정을 위한 핵산 탐침은 본 발명자들에 의해 직접 동정된 각 세균의 23S rRNA 유전자 및 ITS의 전체 염기 서열(서열번호 1 내지 28)로부터 유도되어 이의 기원이 되는 세균의 23S rRNA 또는 ITS와 특정적으로 혼성화하고 그 외의 다른 유기체의 핵산과는 교차 반응하지 않는 특정 염기 서열을 갖는다. 상기 (29) 내지 (44)의 세균의 경우는 공지된 23S rRNA 유전자로부터 유도되어 이의 기원이 되는 세균의 23S rRNA 유전자와 특정적으로 혼성화하고 그 외 다른 유기체의 핵산과는 교차 반응하지 않는 특정 염기 서열을 갖는다. 상기 (45) 및 (46)의 진균의 경우는 공지된 18S rRNA 유전자로부터 유도되어 이의 기원이 되는 진균의 18S rRNA 유전자와 특정적으로 혼성화하고 그 외 다른 유기체의 핵산과는 교차 반응하지 않는 염기 서열을 갖는다.The inventors have developed probes with sequences that specifically hybridize with the rRNA genes of each pathogen and do not cross-react with nucleic acids of other organisms, and also fabricate DNA chips incorporating these probes and conduct them through clinical trials. The object of the present invention was achieved by confirming the specificity of each. The nucleic acid probe for detecting and identifying each bacterium of (1) to (28) is derived from the 23S rRNA gene of each bacterium directly identified by the present inventors and the entire nucleotide sequence of ITS (SEQ ID NOS: 1 to 28). It has a specific base sequence that hybridizes specifically with the 23S rRNA or ITS of the bacteria of its origin and does not cross react with nucleic acids of other organisms. In the case of the bacteria of (29) to (44), a specific base which is derived from a known 23S rRNA gene and specifically hybridizes with the 23S rRNA gene of the bacterium of its origin and does not cross-react with nucleic acids of other organisms Has a sequence. In the case of the fungi of (45) and (46), the base sequence derived from the known 18S rRNA gene and specifically hybridized with the 18S rRNA gene of the fungus of which its origin is used and does not cross-react with the nucleic acid of other organisms Has

한 관점으로, 본 발명은 상기 (1) 내지 (28)의 세균의 23S rRNA 유전자와 ITS의 전체 염기 서열로서 각각 서열번호 1 내지 28로 기술된 염기 서열을 갖는 핵산 분자를 제공한다.In one aspect, the present invention provides a nucleic acid molecule having the nucleotide sequences set forth in SEQ ID NOS: 1 to 28, respectively, as the entire nucleotide sequence of the 23S rRNA gene and the ITS of the bacteria of (1) to (28).

다른 관점(1-i-a)으로, 본 발명은 아시네토박터 바우마니 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (1-i-a), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from the 23S rRNA of Acinetobacter Baumani:

TGATGGAACTTGCTT (Acti004, 서열번호 29);TGATGGAACTTGCTT (Acti004, SEQ ID NO: 29);

AGGGCACACATAATG (Acti23S01, 서열번호 30); 또는AGGGCACACATAATG (Acti23S01, SEQ ID NO: 30); or

ACGCTGTTGTTGGTG (Acti23S02, 서열번호 31).ACGCTGTTGTTGGTG (Acti23S02, SEQ ID NO: 31).

다른 관점(1-i-b)으로, 본 발명은 아시네토박터 바우마니 균의 ITS로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (1-i-b), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from the ITS of Acinetobacter Baumani:

ATACACAGTACTTCG (Acti3, 서열번호 32);ATACACAGTACTTCG (Acti3, SEQ ID NO: 32);

ATAGTGTTGCAAGGC (Acti002, 서열번호 33); 또는ATAGTGTTGCAAGGC (Acti002, SEQ ID NO: 33); or

TGAAAAGCCAGGGGA (Acti003, 서열번호 34).TGAAAAGCCAGGGGA (Acti003, SEQ ID NO: 34).

다른 관점(1-ii)으로, 본 발명은 상기 서열번호 29 내지 34중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 아시네토박터 바우마니 균의 검출용 핵산 탐침을 제공한다.In another aspect (1-ii), the present invention provides a nucleic acid probe for detecting Acinetobacter Baumani, comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 29-34.

다른 관점(1-iii)으로, 본 발명은 상기 서열번호 29 내지 34중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 아시네토박터 바우마니 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (1-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Acinetobacter Baumani, comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 29-34.

다른 관점(1-iv)으로, 본 발명은 (a) 상기 서열번호 29 내지 34중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 아시네토박터 바우마니 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 아시네토박터 바우마니 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (1-iv), the present invention provides a composition comprising (a) a nucleic acid probe for detecting Acinetobacter Baumani, comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 29 to 34; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a previously formed hybrid, thereby providing a kit for detecting and identifying Acinetobacter Baumani bacteria in a biological sample.

다른 관점(1-v)으로, 본 발명은 상기 서열번호 29 내지 34중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 아시네토박터 바우마니 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (1-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting Acinetobacter Baumani, comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 29 to 34, is immobilized on a solid support. To provide.

다른 관점(1-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 29 내지 34중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 아시네토박터 바우마니 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 아시네토박터 바우마니 균을 검출 및 동정하는 방법을 제공한다.In another aspect (1-vi), the present invention is directed to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) a composition comprising a nucleic acid probe for detecting Acinetobacter Baumani, comprising the nucleic acid molecule of any one of SEQ ID NOs: 29 to 34 as the polynucleic acid of steps (a) and (b); Contacting under appropriate hybridization and washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying acinetobacter Baumani bacteria in the biological sample.

다른 관점(2-i-a)으로, 본 발명은 언에어로비오스피리룸 숙시니시프로더센스 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (2-i-a), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from the 23S rRNA of Unaerobiopyrirum succinisiprodersense bacterium:

TGACTCGTGCCCATG (Anas001, 서열번호 45);TGACTCGTGCCCATG (Anas001, SEQ ID NO: 45);

TACCGGGGTTAAAAG (Anas002, 서열번호 46);TACCGGGGTTAAAAG (Anas002, SEQ ID NO: 46);

ATCAGTGATCTGAGA (Anas003, 서열번호 47);ATCAGTGATCTGAGA (Anas003, SEQ ID NO: 47);

GAGACGAAGCACCAT (Anas004, 서열번호 48);GAGACGAAGCACCAT (Anas004, SEQ ID NO: 48);

AGTTGATACAGGTAG (Anas011, 서열번호 49);AGTTGATACAGGTAG (Anas011, SEQ ID NO: 49);

GGCCCCATCCGGGGT(Anas013, 서열번호 50); 또는GGCCCCATCCGGGGT (Anas013, SEQ ID NO: 50); or

CAGTTGGAAGCAGAG (Anas23S03, 서열번호 51).CAGTTGGAAGCAGAG (Anas23S03, SEQ ID NO: 51).

다른 관점(2-ii-b)으로, 본 발명은 언에어로비오스피리룸 숙시니시프로더센스 균의 ITS로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (2-ii-b), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following nucleotide sequences derived from the ITS of the unaerobic pyrirum succinicis deferens bacteria:

GTTCTTGATTCATTGC (Anas005, 서열번호 52);GTTCTTGATTCATTGC (Anas005, SEQ ID NO: 52);

CAGCCCAAAAGTTGA (Anas008, 서열번호 53);CAGCCCAAAAGTTGA (Anas008, SEQ ID NO: 53);

AAACTGCAGGGCACA (Anas009, 서열번호 54); 또는AAACTGCAGGGCACA (Anas009, SEQ ID NO: 54); or

ATACTACCTGACGAC (Anas010, 서열번호 55).ATACTACCTGACGAC (Anas010, SEQ ID NO: 55).

다른 관점(2-ii)으로, 본 발명은 상기 서열번호 45 내지 55중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 언에어로비오스피리룸 숙시니시프로더센스 균의 검출용 핵산 탐침을 제공한다.In another aspect (2-ii), the present invention provides a nucleic acid probe for detecting an aerobic pyrirum succinicis prodersense bacterium comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 45 to 55. .

다른 관점(2-iii)으로, 본 발명은 상기 서열번호 45 내지 55중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 언에어로비오스피리룸 숙시니시프로더센스 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (2-iii), the present invention relates to a composition comprising a nucleic acid probe for detecting an aerobic pyrirum succinicide prosthesis bacterium comprising the nucleic acid molecule of any one of SEQ ID NOs: 45 to 55. To provide.

다른 관점(2-iv)으로, 본 발명은 (a) 상기 서열번호 45 내지 55중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 언에어로비오스피리룸 숙시니시프로더센스 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분: 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 언에어로비오스피리룸 숙시니시프로더센스 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (2-iv), the present invention provides (a) a nucleic acid probe for detecting an aerobic pyrirum succinicis prodersense bacterium comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 45 to 55. Composition comprising; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing a hybrid formed under suitable washing conditions or the components necessary to produce such a solution: and (e) optionally means for detecting the hybrid formed previously. Provided are kits for detecting and identifying room succinic nitrodersens bacteria.

관점(2-v)으로, 본 발명은 상기 서열번호 45 내지 55중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 언에어로비오스피리룸 숙시니시프로더센스 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In view of (2-v), the present invention provides a nucleic acid probe for detecting an aerobic pyrirum succinicis prodercene bacterium comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 45 to 55. Provide an immobilized DNA chip.

다른 관점(2-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 45 내지 55중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 언에어로비오스피리룸 숙시니시프로더센스 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 언에어로비오스피리룸 숙시니시프로더센스 균을 검출 및 동정하는 방법을 제공한다.In another aspect (2-vi), the present invention is directed to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) a nucleic acid for detecting an aerobic pyriroom succinicis prodersense bacterium comprising the nucleic acid molecule comprising any one of the nucleotide sequences of SEQ ID NOs: 45 to 55 in the polynucleic acid of steps (a) and (b) Contacting the composition comprising the probe with appropriate hybridization and washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying the strains present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying un-aerobiopyrirum succinicisprodersense bacteria in the biological sample. do.

다른 관점(3-i)으로, 본 발명은 박테로이데스 프라질리스 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (3-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from Bacteroides praxilli 's 23S rRNA:

GTCGAACCTGACAGT (Bf011, 서열번호 78).GTCGAACCTGACAGT (Bf011, SEQ ID NO: 78).

다른 관점(3-ii)으로, 본 발명은 상기 서열번호 78의 염기 서열을 포함한 핵산 분자를 포함하는 박테로이데스 프라질리스 균의 검출용 핵산 탐침을 제공한다.In another aspect (3-ii), the present invention provides a nucleic acid probe for detecting Bacteroides prasilis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 78.

다른 관점(3-iii)으로, 본 발명은 상기 서열번호 78의 염기 서열을 포함한 핵산 분자를 포함하는 박테로이데스 프라질리스 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (3-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Bacteroides prasilis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 78.

다른 관점(3-iv)으로, 본 발명은 (a) 상기 서열번호 78의 염기 서열을 포함한 핵산 분자를 포함하는 박테로이데스 프라질리스 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 박테로이데스 프라질리스균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (3-iv), the present invention provides a composition comprising (a) a nucleic acid probe for detecting Bacteroides prazilis, comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 78; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, to provide a kit for detecting and identifying Bacteroides praxilis in a biological sample.

다른 관점(3-v)으로, 본 발명은 상기 서열번호 78의 염기 서열을 포함한 핵산 분자를 포함하는 박테로이데스 프라질리스 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (3-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting Bacteroides prasilis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 78 is fixed to a solid support.

다른 관점(3-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 78의 염기 서열을 포함한 핵산 분자를 포함하는 박테로이데스 프라질리스 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 박테로이데스 프라질리스 균을 검출 및 동정하는 방법을 제공한다.In another aspect (3-vi), the present invention relates to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) appropriate hybridization and washing conditions with a composition comprising a nucleic acid probe for detecting Bacteroides prasilis bacteria comprising the nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 78, wherein the polynucleic acid of step (a) and (b) Contacting under; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Bacteroides pragillis bacteria in the biological sample.

다른 관점(4-i-a)으로, 본 발명은 카디오박테리움 호미니스 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (4-i-a), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from the 23S rRNA of Cardiobacterium homominis:

AACCCTGGTGAAGGG (Car 006, 서열번호 93);AACCCTGGTGAAGGG (Car 006, SEQ ID NO: 93);

ATATGAAGATATGTG (Car 007, 서열번호 94);ATATGAAGATATGTG (Car 007, SEQ ID NO: 94);

TAGATTGACTTACGG (Car 008, 서열번호 95);TAGATTGACTTACGG (Car 008, SEQ ID NO: 95);

GTAAAGTTTTACTAC (Car 009, 서열번호 96); 또는GTAAAGTTTTACTAC (Car 009, SEQ ID NO: 96); or

CCAGCACACTGTTGG (Car2, 서열번호 97).CCAGCACACTGTTGG (Car2, SEQ ID NO: 97).

다른 관점(4-i-b)으로, 본 발명은 카디오박테리움 호미니스 균의 ITS로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (4-i-b), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from the ITS of Cardiobacterium hominis bacteria:

AAAGAGAGAACAGCA (Car3(CarI), 서열번호 98);AAAGAGAGAACAGCA (Car3 (CarI), SEQ ID NO: 98);

TTGGCGACAACAGGC (Car001, 서열번호 99);TTGGCGACAACAGGC (Car001, SEQ ID NO: 99);

GCCCCGGGAAGCTGA (Car002, 서열번호 100);GCCCCGGGAAGCTGA (Car002, SEQ ID NO: 100);

TAGACTGCGGAAGCG (Car003, 서열번호 101); 또는TAGACTGCGGAAGCG (Car003, SEQ ID NO: 101); or

AATTAAGTTGCGTAT (Car004, 서열번호 102).AATTAAGTTGCGTAT (Car004, SEQ ID NO: 102).

다른 관점(4-ii)으로, 본 발명은 상기 서열번호 93 내지 102중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 카디오박테리움 호미니스 균의 검출용 핵산 탐침을 제공한다.In another aspect (4-ii), the present invention provides a nucleic acid probe for detecting Cardiobacterium hominis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 93-102.

다른 관점(4-iii)으로, 본 발명은 상기 서열번호 93 내지 102중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 카디오박테리움 호미니스 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (4-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Cardiobacterium homominis comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 93-102.

다른 관점(4-iv)으로, 본 발명은 (a) 상기 서열번호 93 내지 102중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 카디오박테리움 호미니스 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 카디오박테리움 호미니스 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (4-iv), the present invention provides a composition comprising: (a) a composition comprising a nucleic acid probe for detecting Cardiobacterium hominis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 93 to 102; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Cardiobacterium hominis bacteria in a biological sample.

다른 관점(v)으로, 본 발명은 상기 서열번호 93 내지 102중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 카디오박테리움 호미니스 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (v), the present invention provides a DNA chip in which a nucleic acid probe for detecting Cardiobacterium homominis comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 93 to 102 is fixed to a solid support. do.

다른 관점(vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 93 내지 102중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 카디오박테리움 호미니스 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 카디오박테리움 호미니스 균을 검출 및 동정하는 방법을 제공한다.In another aspect (vi), the present invention relates to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) a composition comprising a nucleic acid probe for detecting a Cardiobacterium hominis bacterium comprising the nucleic acid molecule of any one of SEQ ID NOs: 93 to 102 as the polynucleic acid of steps (a) and (b); Contacting under appropriate hybridization and washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Cardiobacterium hominis bacteria in the biological sample.

다른 관점(5-i-a)으로, 본 발명은 크리서박테리움 메닌고셉티쿰 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (5-i-a), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from 23S rRNA of Chrysbacterium meningocystum bacillus:

GGCATATTTAGATGA (Chr23S04, 서열번호 105).GGCATATTTAGATGA (Chr23S04, SEQ ID NO: 105).

다른 관점(5-i-b)으로, 본 발명은 크리서박테리움 메닌고셉티쿰 균의 ITS로부터 유도된 하기 염기 서열을 포함한 분리된 핵산 분자를 제공한다:In another aspect (5-i-b), the present invention provides an isolated nucleic acid molecule comprising the following base sequence derived from ITS of Chrysbacterium meningocystum fungus:

CTTAGGTGATCACTT (Chr001, 서열번호 106);CTTAGGTGATCACTT (Chr001, SEQ ID NO: 106);

TAACCCCTTAGATTA (Chr003, 서열번호 107);TAACCCCTTAGATTA (Chr003, SEQ ID NO: 107);

TCAAACCTCAAACTA (Chr004, 서열번호 108); 또는TCAAACCTCAAACTA (Chr004, SEQ ID NO: 108); or

AAGAAATCGAAGAGA (Chr005, 서열번호 109).AAGAAATCGAAGAGA (Chr005, SEQ ID NO: 109).

다른 관점(5-ii)으로, 본 발명은 상기 서열번호 105 내지 109중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 크리서박테리움 메닌고셉티쿰 균의 검출용 핵산 탐침을 제공한다.In another aspect (5-ii), the present invention provides a nucleic acid probe for detecting Chrysbacterium meningocceptum bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 105 to 109.

다른 관점(5-iii)으로, 본 발명은 상기 서열번호 105 내지 109중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 크리서박테리움 메닌고셉티쿰 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (5-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Chrysbacterium meningocceptum bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 105 to 109. .

다른 관점(5-iv)으로, 본 발명은 (a) 상기 서열번호 105 내지 109중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 크리서박테리움 메닌고셉티쿰 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 크리서박테리움 메닌고셉티쿰 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (5-iv), the present invention provides a composition comprising (a) a nucleic acid probe for detecting Chrysbacterium meningocystum bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 105 to 109. ; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Chrysbacterium meningocystum bacteria in a biological sample.

다른 관점(5-v)으로, 본 발명은 상기 서열번호 105 내지 109중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 크리서박테리움 메닌고셉티쿰 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (5-v), the present invention provides a method for immobilizing a nucleic acid probe for detection of bacteria of the bacterium bacterium meningocceptum comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 105 to 109 on a solid support. Provide a DNA chip.

다른 관점(5-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 105 내지 109중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 크리서박테리움 메닌고셉티쿰 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 크리서박테리움 메닌고셉티쿰 균을 검출 및 동정하는 방법을 제공한다.In another aspect (5-vi), the present invention is directed to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) a nucleic acid probe for detecting the Krysarbacterium meningocystum bacterium comprising the nucleic acid molecule of any one of SEQ ID NOs: 105 to 109 in the polynucleic acid of steps (a) and (b); Contacting the composition under appropriate hybridization and washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Chrysbacterium meningocystum bacteria in the biological sample.

다른 관점(6-i)으로, 본 발명은 클로스트리디움 람모섬 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (6-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from the 23S rRNA of Clostridium lammosomes:

CCAGTGTGTGAGGAG (C.ramosa04, 서열번호 115); 또는CCAGTGTGTGAGGAG (C.ramosa04, SEQ ID NO: 115); or

CCCGGGAAGGGGAGT (C.ramo 004, 서열번호 116).CCCGGGAAGGGGAGT (C.ramo 004, SEQ ID NO: 116).

다른 관점(6-ii)으로, 본 발명은 상기 서열번호 115 또는 116의 염기 서열을 포함한 핵산 분자를 포함하는 클로스트리디움 람모섬 균의 검출용 핵산 탐침을 제공한다.In another aspect (6-ii), the present invention provides a nucleic acid probe for the detection of Clostridium rhammosome bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 115 or 116.

다른 관점(6-iii)으로, 본 발명은 상기 서열번호 115 또는 116의 염기 서열을 포함한 핵산 분자를 포함하는 클로스트리디움 람모섬 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (6-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Clostridium lammosome bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 115 or 116.

다른 관점(6-iv)으로, 본 발명은 (a) 상기 서열번호 115 또는 116의 염기 서열을 포함한 핵산 분자를 포함하는 클로스트리디움 람모섬 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 클로스트리디움 람모섬 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (6-iv), the present invention (a) composition comprising a nucleic acid probe for the detection of Clostridium rhammosome bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 115 or 116; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Clostridium rhammosomes in a biological sample.

다른 관점(6-v)으로, 본 발명은 상기 서열번호 115 또는 116의 염기 서열을 포함한 핵산 핵산 분자를 포함하는 클로스트리디움 람모섬 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (6-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting Clostridium rammosome bacteria comprising a nucleic acid nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 115 or 116 is fixed to a solid support. do.

다른 관점(6-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 115 또는 116의 염기 서열을 포함한 핵산 분자를 포함하는 클로스트리디움 람모섬 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 클로스트리디움 람모섬 균을 검출 및 동정하는 방법을 제공한다.In another aspect (6-vi), the present invention is directed to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) appropriate hybridization of the polynucleic acid of steps (a) and (b) with a composition comprising a nucleic acid probe for the detection of Clostridium rhammosome bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 115 or 116 and Contacting under washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Clostridium rhammosome in the biological sample.

다른 관점(7-i)으로, 본 발명은 코마모나스 아시도보란스 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (7-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from the 23S rRNA of Coomamonas ashidoboranth bacteria:

TAGGGCGTCCAGTCG (Com 004, 서열번호 124);TAGGGCGTCCAGTCG (Com 004, SEQ ID NO: 124);

CGCAGAGTACAGCTT (Com 005, 서열번호 125);CGCAGAGTACAGCTT (Com 005, SEQ ID NO: 125);

GTACCGATGTGTAGT (Com 006, 서열번호 126);GTACCGATGTGTAGT (Com 006, SEQ ID NO: 126);

GAACTTGAACAAAGG (Com 007, 서열번호 127);GAACTTGAACAAAGG (Com 007, SEQ ID NO: 127);

TGTGCTAGAGAAAAG (Coma2, 서열번호 128); 또는TGTGCTAGAGAAAAG (Coma2, SEQ ID NO: 128); or

ATCCGCCGGGCTTAG (Coma3, 서열번호 129).ATCCGCCGGGCTTAG (Coma3, SEQ ID NO: 129).

다른 관점(7-ii)으로, 본 발명은 상기 서열번호 124 내지 129중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 코마모나스 아시도보란스 균의 검출용 핵산 탐침을 제공한다.In another aspect (7-ii), the present invention provides a nucleic acid probe for the detection of Komamonas ashidoboranth bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 124 to 129.

다른 관점(7-iii)으로, 본 발명은 상기 서열번호 124 내지 129중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 코마모나스 아시도보란스 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (7-iii), the present invention provides a composition comprising a nucleic acid probe for detection of Komamonas ashidoboranth bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 124 to 129.

다른 관점(7-iv)으로, 본 발명은 (a) 상기 서열번호 124 내지 129중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 코마모나스 아시도보란스 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 코마모나스 아시도보란스 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (7-iv), the present invention provides a composition comprising: (a) a nucleic acid probe for the detection of Komamonas asidoborance bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 124 to 129; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying the bacteria of Comamonas asidoborance in a biological sample.

다른 관점(7-v)으로, 본 발명은 상기 서열번호 124 내지 129중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 코마모나스 아시도보란스 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (7-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting the comamonas ashidoboranth bacterium comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 124 to 129 is fixed to a solid support. To provide.

다른 관점(7-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 124 내지 129중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 코마모나스 아시도보란스 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 코마모나스 아시도보란스 균을 검출 및 동정하는 방법을 제공한다.In another aspect (7-vi), the present invention relates to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) a composition comprising a nucleic acid probe for detecting Komamonas ashidoboranth bacteria comprising the nucleic acid molecule of any one of SEQ ID NOs: 124 to 129, wherein the polynucleic acid of step (a) and (b) Contacting under appropriate hybridization and washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method of detecting and identifying the coma monoma asidoborance bacteria in the biological sample.

다른 관점(8-i)으로, 본 발명은 코리네박테리움 디프테리애 균으로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (8-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following nucleotide sequences derived from Corynebacterium diphtheria bacteria:

ACCATCTTCCCAAGG (C.diph003, 서열번호 135).ACCATCTTCCCAAGG (C.diph003, SEQ ID NO: 135).

다른 관점(8-ii)으로, 본 발명은 상기 서열번호 135의 염기 서열을 포함한 핵산 분자를 포함하는 코리네박테리움 디프테리애 균의 검출용 핵산 탐침을 제공한다.In another aspect (8-ii), the present invention provides a nucleic acid probe for detecting Corynebacterium diphtheria bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 135.

다른 관점(8-iii)으로, 본 발명은 상기 서열번호 135의 염기 서열을 포함한 핵산 분자를 포함하는 코리네박테리움 디프테리애 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (8-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Corynebacterium diphtheria bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 135.

다른 관점(8-iv)으로, 본 발명은 (a) 상기 서열번호 135의 염기 서열을 포함한 핵산 분자를 포함하는 코리네박테리움 디프테리애 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 코리네박테리움 디프테리애 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (8-iv), the present invention provides a composition comprising (a) a nucleic acid probe for detecting Corynebacterium diphtheria bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 135; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Corynebacterium diphtheria bacteria in a biological sample.

다른 관점(8-v)으로, 본 발명은 상기 서열번호 135의 염기 서열을 포함한 핵산 분자를 포함하는 코리네박테리움 디프테리애 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (8-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting Corynebacterium diphtheria bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 135 is fixed to a solid support. .

다른 관점(8-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 135의 염기 서열을 포함한 핵산 분자를 포함하는 코리네박테리움 디프테리애 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 코리네박테리움 디프테리애 균을 검출 및 동정하는 방법을 제공한다.In another aspect (8-vi), the present invention provides a process for (a) isolating and / or concentrating polynucleic acid, optionally present in a biological sample, and (b) optionally amplifying the polynucleic acid with appropriate forward and reverse primer pairs; (c) appropriate hybridization of the polynucleic acid of steps (a) and (b) with a composition comprising a nucleic acid probe for detecting Corynebacterium diphtheria bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 135; and Contacting under washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Corynebacterium diphtheria bacteria in the biological sample.

다른 관점(9-i)으로, 본 발명은 크레브시엘라 옥시토카 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (9-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from the 23S rRNA of the Crevciella oxytoca strain:

GAACGTTACTAACGC (Ko001, 서열번호 142).GAACGTTACTAACGC (Ko001, SEQ ID NO: 142).

다른 관점(9-ii)으로, 본 발명은 상기 서열번호 142의 염기 서열을 포함한 핵산 분자를 포함하는 크레브시엘라 옥시토카 균의 검출용 핵산 탐침을 제공한다. In another aspect (9-ii), the present invention provides a nucleic acid probe for detecting Crebsiella oxytoca bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 142.

다른 관점(9-iii)으로, 본 발명은 상기 서열번호 142의 염기 서열을 포함한 핵산 분자를 포함하는 크레브시엘라 옥시토카 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (9-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Crebesciella oxytoca bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 142.

다른 관점(9-iv)으로, 본 발명은 (a) 상기 서열번호 142의 염기 서열을 포함한 핵산 분자를 포함하는 크레브시엘라 옥시토카 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 크레브시엘라 옥시토카 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (9-iv), the present invention provides a composition comprising (a) a nucleic acid probe for the detection of Crebsiella oxytoca bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 142; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Crevciella oxytoca bacteria in a biological sample.

다른 관점(9-v)으로, 본 발명은 상기 서열번호 142의 염기 서열을 포함한 핵산 분자를 포함하는 크레브시엘라 옥시토카 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (9-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting Crebesciella oxytoca bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 142 is fixed to a solid support.

다른 관점(9-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 142의 염기 서열을 포함한 핵산 분자를 포함하는 크레브시엘라 옥시토카 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 크레브시엘라 옥시토카 균을 검출 및 동정하는 방법을 제공한다.In another aspect (9-vi), the present invention is directed to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) appropriate hybridization and washing of the polynucleic acid of steps (a) and (b) with a composition comprising a nucleic acid probe for the detection of the Crebessiella oxytoca bacterium comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 142. Contacting under conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Crevsciella oxytoca in the biological sample.

다른 관점(10-i-a)으로, 본 발명은 오크로박트룸 안트로피 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (10-i-a), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following nucleotide sequences derived from the 23S rRNA of Okrobacrum antropy:

GGACCAGGCCAGTGG (Ochr04, 서열번호 151); 또는GGACCAGGCCAGTGG (Ochr04, SEQ ID NO: 151); or

GACCAGGCCAGTGGC (Ochr05, 서열번호 152).GACCAGGCCAGTGGC (Ochr05, SEQ ID NO: 152).

다른 관점(10-i-b)으로, 본 발명은 오크로박트룸 안트로피 균의 ITS로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (10-i-b), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following nucleotide sequences derived from the ITS of Okrobacrum antropy:

GTTGATTGACACTTG (Ochr004, 서열번호 153);GTTGATTGACACTTG (Ochr004, SEQ ID NO: 153);

TACCGCTCACGAGCC (Ochr005, 서열번호 154); 또는TACCGCTCACGAGCC (Ochr005, SEQ ID NO: 154); or

GGGTCCGGAGGTTCA (Ochr007, 서열번호 155).GGGTCCGGAGGTTCA (Ochr007, SEQ ID NO: 155).

다른 관점(10-ii)으로, 본 발명은 상기 서열번호 151 내지 155중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 오크로박트룸 안트로피 균의 검출용 핵산 탐침을 제공한다.In another aspect (10-ii), the present invention provides a nucleic acid probe for the detection of Ocrobacrum antropy bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 151 to 155.

다른 관점(10-iii)으로, 본 발명은 상기 서열번호 151 내지 155중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 오크르박트룸 안트로피 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (10-iii), the present invention provides a composition comprising a nucleic acid probe for detecting the bacterium Okractrum, comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 151 to 155.

다른 관점(10-iv)으로, 본 발명은 (a) 상기 서열번호 151 내지 155중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 오크로박트룸 안트로피 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 오크로박트룸 안트로피 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (10-iv), the present invention provides a composition comprising (a) a nucleic acid probe for the detection of Ocrobacrum antropy bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 151 to 155; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying the okrobacterium antropy bacteria in a biological sample.

다른 관점(10-v)으로, 본 발명은 상기 서열번호 151 내지 155중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 오크로박트룸 안트로피 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (10-v), the present invention provides a DNA in which a nucleic acid probe for detecting Ocrobacrum antropy, including a nucleic acid molecule comprising any one of SEQ ID NOs: 151 to 155, is immobilized on a solid support. Provide chips.

다른 관점(10-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 151 내지 155중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 오크로박트룸 안트로피 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 오크로박트룸 안트로피 균을 검출 및 동정하는 방법을 제공한다.In another aspect (10-vi), the present invention is directed to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) a composition comprising a nucleic acid probe for the detection of Ocrobacrum antropy bacteria, wherein the polynucleic acid of steps (a) and (b) comprises a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 151 to 155 Contacting with appropriate hybridization and washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying the strains present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying the Okrobacrum antropy bacteria in the biological sample.

다른 관점(11-i)으로, 본 발명은 펩토스트렙토코코스 프레보티 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (11-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from the 23S rRNA of peptostreptococcus prevoti bacteria:

ACTAGGGAGAGCTCA (Pep002, 서열번호 166);ACTAGGGAGAGCTCA (Pep002, SEQ ID NO: 166);

GCTTAGTAAAGCAAG (Pep003, 서열번호 167);GCTTAGTAAAGCAAG (Pep003, SEQ ID NO: 167);

TACTAACATGTGACC (pep004, 서열번호 168);TACTAACATGTGACC (pep004, SEQ ID NO: 168);

AAGCAGAGAGAGCTC (Pep005, 서열번호 169);AAGCAGAGAGAGCTC (Pep005, SEQ ID NO: 169);

CGAACGGTGAGGCCG (Pep006, 서열번호 170);CGAACGGTGAGGCCG (Pep006, SEQ ID NO: 170);

GTAGATGTTGATTAT (Pep007, 서열번호 171);GTAGATGTTGATTAT (Pep007, SEQ ID NO: 171);

GTCGAATCATCTGGG (Pep23S02, 서열번호 172); 또는GTCGAATCATCTGGG (Pep23S02, SEQ ID NO: 172); or

TAAAACGTATCGGAT (Pep23S03, 서열번호 173).TAAAACGTATCGGAT (Pep23S03, SEQ ID NO: 173).

다른 관점(11-ii)으로, 본 발명은 상기 서열번호 166 내지 173중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 펩토스트렙토코코스 프레보티 균의 검출용 핵산 탐침을 제공한다.In another aspect (11-ii), the present invention provides a nucleic acid probe for detecting peptostreptococcus prevoti bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 166 to 173.

다른 관점(11-iii)으로, 본 발명은 상기 서열번호 166 내지 173중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 펩토스트렙토코코스 프레보티 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (11-iii), the present invention provides a composition comprising a nucleic acid probe for detecting peptostreptococcus prevoti bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 166 to 173.

다른 관점(11-iv)으로, 본 발명은 (a) 상기 서열번호 166 내지 173중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 펩토스트렙토코코스 프레보티 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 펩토스트렙토코코스 프레보티 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (11-iv), the present invention provides a composition comprising (a) a nucleic acid probe for detecting peptostreptococcus prevoti bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 166 to 173; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying peptostreptococcus prevoti bacteria in biological samples.

다른 관점(11-v)으로, 본 발명은 상기 서열번호 166 내지 173중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 펩토스트렙토코코스 프레보티 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (11-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting peptostreptococcus prevoti bacteria comprising the nucleic acid molecule of any one of SEQ ID NOs: 166 to 173 is fixed to a solid support. To provide.

다른 관점(11-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 166 내지 173중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 펩토스트렙토코코스 프레보티 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 펩토스트렙토코코스 프레보티 균을 검출 및 동정하는 방법을 제공한다.In another aspect (11-vi), the present invention is directed to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) a composition comprising a nucleic acid probe for detecting peptostreptococcus prevoti bacteria comprising the nucleic acid molecule of any one of SEQ ID NOs: 166 to 173 in the polynucleic acid of steps (a) and (b); Contacting under appropriate hybridization and washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying peptostreptococcus prevoti bacteria in the biological sample.

다른 관점(12-i-a)으로, 본 발명은 포피로모나스 진지발리스 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (12-i-a), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following nucleotide sequences derived from the 23S rRNA of Popiromonas genjivalis bacterium:

AGTTGGTGAGCGAGC (Por 003, 서열번호 178); 또는AGTTGGTGAGCGAGC (Por 003, SEQ ID NO: 178); or

CTGAGCTGTCGTGCA (Por23S08, 서열번호 179).CTGAGCTGTCGTGCA (Por23S08, SEQ ID NO: 179).

다른 관점(12-i-b)으로, 본 발명은 포피로모나스 진지발리스 균의 ITS로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (12-i-b), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from the ITS of Popiromonas jinjivalis bacterium:

GTTTTTGTGAGTGGA (Por 001, 서열번호 180); 또는GTTTTTGTGAGTGGA (Por 001, SEQ ID NO: 180); or

TGATGGGTGGGGTTG (Por 002, 서열번호 181).TGATGGGTGGGGTTG (Por 002, SEQ ID NO: 181).

다른 관점(12-ii)으로, 본 발명은 상기 서열번호 178 내지 181중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 포피로모나스 진지발리스 균의 검출용 핵산 탐침을 제공한다.In another aspect (12-ii), the present invention provides a nucleic acid probe for the detection of P. pylorimonas genjivalis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 178 to 181.

다른 관점(12-iii)으로, 본 발명은 상기 서열번호 178 내지 181중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 포피로모나스 진지발리스 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (12-iii), the present invention provides a composition comprising a nucleic acid probe for detection of P. pylorimonas genjivalis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 178 to 181.

다른 관점(12-iv)으로, 본 발명은 (a) 상기 서열번호 178 내지 181 중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 포피로모나스 진지발리스 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 포피로모나스 진지발리스 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (12-iv), the present invention provides a composition comprising (a) a nucleic acid probe for detecting P. pylorimonas genjivalis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 178 to 181; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, to provide a kit for detecting and identifying Popiromonas zybacilli bacteria in a biological sample.

다른 관점(12-v)으로, 본 발명은 상기 서열번호 178 내지 181중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 포피로모나스 진지발리스 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (12-v), the present invention relates to a DNA in which a nucleic acid probe for detecting P. pylorimonas genjivalis bacteria, comprising a nucleic acid molecule comprising any one of SEQ ID NOs: 178 to 181, is immobilized on a solid support. Provide chips.

다른 관점(12-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 178 내지 181중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 포피로모나스 진지발리스 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 포피로모나스 진지발리스 균을 검출 및 동정하는 방법을 제공한다.In another aspect (12-vi), the present invention is directed to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) a composition comprising a nucleic acid probe for detecting P. pylorimonas genjivalis bacterium comprising the nucleic acid molecule of any one of SEQ ID NOs: 178 to 181 as the polynucleic acid of steps (a) and (b) Contacting with appropriate hybridization and washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying the strains present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Popiromonas genjivalis bacteria in the biological sample.

다른 관점(13-i)으로, 본 발명은 펩토스트렙토코커스 언애어로비우스 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (13-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from the Peptostreptococcus aeravius bacterium 23S rRNA:

AGGAGGAAGAGAAAG (P.anae003, 서열번호 186); 또는AGGAGGAAGAGAAAG (P.anae003, SEQ ID NO: 186); or

GCGAAAGGAAAAGAG (P.anae004, 서열번호 187).GCGAAAGGAAAAGAG (P.anae004, SEQ ID NO: 187).

다른 관점(13-ii)으로, 본 발명은 상기 서열번호 186 또는 187의 염기 서열을 포함한 핵산 분자를 포함하는 펩토스트렙토코커스 언애어로비우스 균의 검출용 핵산 탐침을 제공한다.In another aspect (13-ii), the present invention provides a nucleic acid probe for the detection of a peptostreptococcus aeravius bacterium comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 186 or 187.

다른 관점(13-iii)으로, 본 발명은 상기 서열번호 186 또는 187의 염기 서열을 포함한 핵산 분자를 포함하는 펩토스트렙토코커스 언애어로비우스 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (13-iii), the present invention provides a composition comprising a nucleic acid probe for detecting peptostreptococcus aerovirus bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 186 or 187.

다른 관점(13-iv)으로, 본 발명은 (a) 상기 서열번호 186 내지 187중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 펩토스트렙토코커스 언애어로비우스 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 펩토스트렙토코커스 언애어로비우스 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (13-iv), the present invention provides a composition comprising (a) a nucleic acid probe for detecting a peptostreptococcus aeravius bacterium comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 186 to 187; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying peptostreptococcus aerobic bacteria in biological samples.

다른 관점(13-v)으로, 본 발명은 상기 서열번호 186 또는 187의 염기 서열을 포함한 핵산 분자를 포함하는 펩토스트렙토코커스 언애어로비우스 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (13-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting peptostreptococcus aeravius bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 186 or 187 is fixed to a solid support. do.

다른 관점(13-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 186 또는 187의 염기 서열을 포함한 핵산 분자를 포함하는 펩토스트렙토코커스 언애어로비우스 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 펩토스트렙토코커스 언애어로비우스 균은 검출 및 동정하는 방법을 제공한다.In another aspect (13-vi), the present invention is directed to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) appropriate hybridization of the polynucleic acid of steps (a) and (b) with a composition comprising a nucleic acid probe for detecting peptostreptococcus aeravius bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 186 or 187 And under washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying the strains present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying peptostreptococcus aerobic bacteria in the biological sample.

다른 관점(14-i)으로, 본 발명은 펩토스트렙토코커스 마그누스 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (14-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from the Peptostreptococcus magnus 23S rRNA:

CATGCAACGATCCGT (P.magn002, 서열번호 190).CATGCAACGATCCGT (P.magn002, SEQ ID NO: 190).

다른 관점(14-ii)으로, 본 발명은 상기 서열번호 190의 염기 서열을 포함한 핵산 분자를 포함하는 펩토스트렙토코커스 마그누스 균의 검출용 핵산 탐침을 제공한다.In another aspect (14-ii), the present invention provides a nucleic acid probe for detecting Peptostreptococcus magnus comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 190.

다른 관점(14-iii)으로, 본 발명은 상기 서열번호 190의 염기 서열을 포함한 핵산 분자를 포함하는 펩토스트렙토코커스 마그누스 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (14-iii), the present invention provides a composition comprising a nucleic acid probe for detecting peptostreptococcus magnus comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 190.

다른 관점(14-iv)으로, 본 발명은 (a) 상기 서열번호 190의 염기 서열을 포함한 핵산 분자를 포함하는 펩토스트렙토코커스 마그누스 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 펩토스트렙토코커스 마그누스 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (14-iv), the present invention provides a composition comprising (a) a nucleic acid probe for detecting peptostreptococcus magnus comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 190; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Peptostreptococcus magnus bacteria in a biological sample.

다른 관점(14-v)으로, 본 발명은 상기 서열번호 190의 염기 서열을 포함한 핵산 분자를 포함하는 펩토스트렙토코커스 마그누스 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (14-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting peptostreptococcus magnus comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 190 is fixed to a solid support.

다른 관점(14-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 190의 염기 서열을 포함한 핵산 분자를 포함하는 펩토스트렙토코커스 마그누스 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 펩토스트렙토코커스 마그누스 균을 검출 및 동정하는 방법을 제공한다.In another aspect (14-vi), the present invention provides a method of (a) isolating and / or concentrating polynucleic acid, optionally present in a biological sample, and (b) optionally amplifying the polynucleic acid with appropriate forward and reverse primer pairs; (c) the polynucleic acid of steps (a) and (b) under appropriate hybridization and washing conditions with a composition comprising a nucleic acid probe for detecting peptostreptococcus magnus comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 190; Contact; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying peptostreptococcus magnus bacteria in the biological sample.

다른 관점(15-i)으로, 본 발명은 푸소박테리움 네크로포룸 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (15-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from the 23S rRNA of Fusobacterium necrophorum bacteria:

TTTCGCAGACGTAAG (fnecro01, 서열번호 193);TTTCGCAGACGTAAG (fnecro01, SEQ ID NO: 193);

GTTTTCTTGCGCTGT (fnecro02, 서열번호 194);GTTTTCTTGCGCTGT (fnecro02, SEQ ID NO: 194);

CCGTATTCATGTCAA (fnecro03, 서열번호 195);CCGTATTCATGTCAA (fnecro03, SEQ ID NO: 195);

CTGCAAGCTATTTCG (fnecro05, 서열번호 196);CTGCAAGCTATTTCG (fnecro05, SEQ ID NO: 196);

CAGACGTAAGCAAAG (fnecro06, 서열번호 197); 또는CAGACGTAAGCAAAG (fnecro06, SEQ ID NO: 197); or

CCTGTATTGGTAGTT (fnecro07, 서열번호 198).CCTGTATTGGTAGTT (fnecro07, SEQ ID NO: 198).

다른 관점(15-ii)으로, 본 발명은 상기 서열번호 193 내지 198중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 푸소박테리움 네크로포룸 균의 검출용 핵산 탐침을 제공한다.In another aspect (15-ii), the present invention provides a nucleic acid probe for detecting Fusobacterium necrophorum bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 193 to 198.

다른 관점(15-iii)으로, 본 발명은 상기 서열번호 193 내지 198중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 푸소박테리움 네크로포룸 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (15-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Fusobacterium necrophorum bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 193 to 198.

다른 관점(15-iv)으로, 본 발명은 (a) 상기 서열번호 193 내지 198중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 푸소박테리움 네크로포룸 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 푸소박테리움 네크로포룸 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (15-iv), the present invention provides a composition comprising (a) a nucleic acid probe for detecting Fusobacterium necrophorum bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 193 to 198; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Fusobacterium necrophorum bacteria in a biological sample.

다른 관점(15-v)으로, 본 발명은 상기 서열번호 193 내지 198중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 푸소박테리움 네크로포룸 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (15-v), the present invention provides a DNA in which a nucleic acid probe for detecting Fusobacterium necrophorum bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 193 to 198 is immobilized on a solid support. Provide chips.

다른 관점(15-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 193 내지 198중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 푸소박테리움 네크로포룸 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 푸소박테리움 네크로포룸 균을 검출 및 동정하는 방법을 제공한다.In another aspect (15-vi), the present invention is directed to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) a composition comprising a nucleic acid probe for the detection of Fusobacterium necrophorum bacteria, wherein the polynucleic acid of steps (a) and (b) comprises a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 193 to 198 Contacting with appropriate hybridization and washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Fusobacterium necrophorum bacteria in the biological sample.

다른 관점(16-i-a)으로, 본 발명은 프로테우스 불가리스 균으로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (16-i-a), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from the Proteus vulgaris bacterium:

AGAGGAGGCTTAGTG (Pvulga04, 서열번호 199).AGAGGAGGCTTAGTG (Pvulga04, SEQ ID NO: 199).

다른 관점(16-i-b)으로, 본 발명은 프로테우스 불가리스 균의 ITS로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (16-i-b), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from the ITS of Proteus vulgaris:

ATACGTGTTATGTGC (Pvulga01, 서열번호 200).ATACGTGTTATGTGC (Pvulga01, SEQ ID NO: 200).

다른 관점(16-ii)으로, 본 발명은 상기 서열번호 199 또는 200의 염기 서열을 포함한 핵산 분자를 포함하는 프로테우스 불가리스 균의 검출용 핵산 탐침을 제공한다.In another aspect (16-ii), the present invention provides a nucleic acid probe for detecting Proteus vulgaris comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 199 or 200.

다른 관점(16-iii)으로, 본 발명은 상기 서열번호 199 또는 200의 염기 서열을 포함한 핵산 분자를 포함하는 프로테우스 불가리스 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (16-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Proteus vulgaris comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 199 or 200.

다른 관점(16-iv)으로, 본 발명은 (a) 상기 서열번호 199 또는 200의 염기 서열을 포함한 핵산 분자를 포함하는 프로테우스 불가리스 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 프로테우스 불가리스 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (16-iv), the present invention provides a composition comprising (a) a nucleic acid probe for detecting Proteus vulgaris comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 199 or 200; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Proteus vulgaris in a biological sample.

다른 관점(16-v)으로, 본 발명은 상기 서열번호 199 또는 200의 염기 서열을 포함한 핵산 분자를 포함하는 프로테우스 불가리스 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (16-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting Proteus vulgaris comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 199 or 200 is fixed to a solid support.

다른 관점(16-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 199 또는 200의 염기 서열을 포함한 핵산 분자를 포함하는 프로테우스 불가리스 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 프로테우스 불가리스 균을 검출 및 동정하는 방법을 제공한다.In another aspect (16-vi), the present invention is directed to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) the polynucleic acid of steps (a) and (b) under appropriate hybridization and washing conditions with a composition comprising a nucleic acid probe for detecting Proteus vulgaris comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 199 or 200 Contact; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Proteus vulgaris in said biological sample.

다른 관점(17-i)으로, 본 발명은 엔테로박터 에어로게네스 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (17-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following nucleotide sequence derived from the Enterobacter aerogenes 23S rRNA:

TTCCGACGGTACAGG (e.aero01, 서열번호 207);TTCCGACGGTACAGG (e.aero01, SEQ ID NO: 207);

GTATCAGTAAGTGCG (e.aero03, 서열번호 208); 및GTATCAGTAAGTGCG (e.aero03, SEQ ID NO: 208); And

TTATCCAGGCAAATC (e.aero04, 서열번호 209).TTATCCAGGCAAATC (e.aero04, SEQ ID NO: 209).

다른 관점(17-ii)으로, 본 발명은 상기 서열번호 207 내지 209중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 엔테로박터 에어로게네스 균의 검출용 핵산 탐침을 제공한다.In another aspect (17-ii), the present invention provides a nucleic acid probe for detecting Enterobacter aerogenes bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 207 to 209.

다른 관점(17-iii)으로, 본 발명은 상기 서열번호 207 내지 209중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 엔테로박터 에어로게네스 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (17-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Enterobacter aerogenes bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 207 to 209.

다른 관점(17-iv)으로, 본 발명은 (a) 상기 서열번호 207 내지 209중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 엔테로박터 에어로게네스 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 엔테로박터 에어로게네스 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (17-iv), the present invention provides a composition comprising (a) a nucleic acid probe for detecting Enterobacter aerogenes comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 207 to 209; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Enterobacter aerogenes in a biological sample.

다른 관점(17-v)으로, 본 발명은 상기 서열번호 207 내지 209중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 엔테로박터 에어로게네스 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (17-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting Enterobacter aerogenes comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 207 to 209 is fixed to a solid support. To provide.

다른 관점(17-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 207 내지 209중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 엔테로박터 에어로게네스 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 엔테로박터 에어로게네스 균을 검출 및 동정하는 방법을 제공한다.In another aspect (17-vi), the present invention is directed to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) a composition comprising a nucleic acid probe for detecting Enterobacter aerogenes bacteria comprising the nucleic acid molecule of any one of SEQ ID NOs: 207 to 209 in the polynucleic acid of steps (a) and (b); Contacting under appropriate hybridization and washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Enterobacter aerogenes in the biological sample.

다른 관점(18-i)으로, 본 발명은 스트렙토코커스 뮤탄스 균으로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (18-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from Streptococcus mutans bacteria:

TAGGTATTCTCTCCT (S.mutan001, 서열번호 212).TAGGTATTCTCTCCT (S.mutan001, SEQ ID NO: 212).

다른 관점(18-ii)으로, 본 발명은 상기 서열번호 212의 염기 서열을 포함한 핵산 분자를 포함하는 스트렙토코커스 뮤탄스 균의 검출용 핵산 탐침을 제공한다.In another aspect (18-ii), the present invention provides a nucleic acid probe for detecting Streptococcus mutans bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 212.

다른 관점(18-iii)으로, 본 발명은 상기 서열번호 212의 염기 서열을 포함한 핵산 분자를 포함하는 스트렙토코커스 뮤탄스 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (18-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Streptococcus mutans bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 212.

다른 관점(18-iv)으로, 본 발명은 (a) 상기 서열번호 212의 염기 서열을 포함한 핵산 분자를 포함하는 스트렙토코커스 뮤탄스 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 스트렙토코커스 뮤탄스 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (18-iv), the present invention provides a composition comprising a nucleic acid probe for detecting Streptococcus mutans bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 212; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Streptococcus mutans bacteria in a biological sample.

다른 관점(18-v)으로, 본 발명은 상기 서열번호 212의 염기 서열을 포함한 핵산 분자를 포함하는 스트렙토코커스 뮤탄스 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (18-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting Streptococcus mutans bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 212 is fixed to a solid support.

다른 관점(18-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 212의 염기 서열을 포함한 핵산 분자를 포함하는 스트렙토코커스 뮤탄스 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 스트렙토코커스 뮤탄스 균을 검출 및 동정하는 방법을 제공한다.In another aspect (18-vi), the present invention is directed to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) the polynucleic acid of steps (a) and (b) under appropriate hybridization and washing conditions with a composition comprising a nucleic acid probe for detecting Streptococcus mutans comprising the nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 212. Contact; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Streptococcus mutans bacteria in the biological sample.

다른 관점(19-i)으로, 본 발명은 킨젤라 킨갭 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (19-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from the 23S rRNA of the Kinzela Kingap strain:

GGTTAGCAAACTGTT (k.king02, 서열번호 218);GGTTAGCAAACTGTT (k.king02, SEQ ID NO: 218);

CCAGTAGGTGGAAAG (k.king03, 서열번호 219);CCAGTAGGTGGAAAG (k.king03, SEQ ID NO: 219);

AACACCGAGACGTGA (k.king04, 서열번호 220); 또는AACACCGAGACGTGA (k.king04, SEQ ID NO: 220); or

TATTCAATGCGATGG (k.king09, 서열번호 221).TATTCAATGCGATGG (k.king09, SEQ ID NO: 221).

다른 관점(19-ii)으로, 본 발명은 상기 서열번호 218 내지 221중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 킨젤라 킨갭 균의 검출용 핵산 탐침을 제공한다.In another aspect (19-ii), the present invention provides a nucleic acid probe for detection of Kinzelella kin gap bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NO: 218 to 221.

다른 관점(19-iii)으로, 본 발명은 상기 서열번호 218 내지 221중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 킨젤라 킨갭 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (19-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Kinzelella kin gap bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 218 to 221.

다른 관점(19-iv)으로, 본 발명은 (a) 상기 서열번호 218 내지 221중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 킨젤라 킨갭 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 킨젤라 킨갭 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (19-iv), the present invention provides a composition comprising: (a) a composition comprising a nucleic acid probe for detecting the Kinzelella kin gap bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 218 to 221; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Kinzelella Kingap bacteria in a biological sample.

다른 관점(19-v)으로, 본 발명은 상기 서열번호 218 내지 221중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 킨젤라 킨갭 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (19-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting a Kinzelella kingap bacterium comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 218 to 221 is fixed to a solid support. do.

다른 관점(19-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 218 내지 221중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 킨젤라 킨갭 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 킨젤라 킨갭 균을 검출 및 동정하는 방법을 제공한다.In another aspect (19-vi), the present invention is directed to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) appropriate hybridization of the polynucleic acid of steps (a) and (b) with a composition comprising a nucleic acid probe for detecting the Kinzelella kin gap bacteria comprising the nucleic acid molecule comprising any one of the nucleotide sequences of SEQ ID NOs: 218 to 221. And under washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Kinzelella kin gap bacteria in the biological sample.

다른 관점(20-i)으로, 본 발명은 박테로이데스 오바투스 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (20-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from Bacterides obatus 23S rRNA:

TAGAAGGAAGCATTC (b.ovatus01, 서열번호 227);TAGAAGGAAGCATTC (b.ovatus01, SEQ ID NO: 227);

CCAATGTTGTTACGG (b.ovatus02, 서열번호 228); 또는CCAATGTTGTTACGG (b.ovatus02, SEQ ID NO: 228); or

TGTAGGACCACGATG (b.ovatus05, 서열번호 229).TGTAGGACCACGATG (b.ovatus05, SEQ ID NO: 229).

다른 관점(20-ii)으로, 본 발명은 상기 서열번호 227 내지 229중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 박테로이데스 오바투스 균의 검출용 핵산 탐침을 제공한다.In another aspect (20-ii), the present invention provides a nucleic acid probe for the detection of Bacterides obatus bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 227 to 229.

다른 관점(20-iii)으로, 본 발명은 상기 서열번호 227 내지 229중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 박테로이데스 오바투스 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (20-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Bacteroides obatus, including a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 227 to 229.

다른 관점(20-iv)으로, 본 발명은 (a) 상기 서열번호 227 내지 229중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 박테로이데스 오바투스 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 박테로이데스 오바투스 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (20-iv), the present invention provides a composition comprising: (a) a composition comprising a nucleic acid probe for detecting Bacteroides obatus comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 227 to 229; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a previously formed hybrid, thereby providing a kit for detecting and identifying Bacteroides obatus bacteria in a biological sample.

다른 관점(20-v)으로, 본 발명은 상기 서열번호 227 내지 229중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 박테로이데스 오바투스 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (20-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting Bacterides obatus bacterium comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 227 to 229 is fixed to a solid support. To provide.

다른 관점(20-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 227 내지 229중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 박테로이데스 오바투스 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 박테로이데스 오바투스 균을 검출 및 동정하는 방법을 제공한다.In another aspect (20-vi), the present invention is directed to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) a composition comprising a nucleic acid probe for detecting Bacterides obatus, wherein the polynucleic acid of steps (a) and (b) comprises a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 227 to 229; Contacting under appropriate hybridization and washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Bacteroides obatatus bacteria in the biological sample.

다른 관점(21-i)으로, 본 발명은 박테로이데스 테타이오타오미크론 균으로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (21-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from Bacterides tetaiotaomicron bacteria:

GCTAACGCAGGGAAC (b.thetaio006, 서열번호 234).GCTAACGCAGGGAAC (b.thetaio006, SEQ ID NO: 234).

다른 관점(21-ii)으로, 본 발명은 상기 서열번호 234의 염기 서열을 포함한 핵산 분자를 포함하는 박테로이데스 테타이오타오미크론 균의 검출용 핵산 탐침을 제공한다.In another aspect (21-ii), the present invention provides a nucleic acid probe for the detection of Bacteroides tetaotaomicron bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 234.

다른 관점(21-iii)으로, 본 발명은 상기 서열번호 234의 염기 서열을 포함한 핵산 분자를 포함하는 박테로이데스 테타이오타오미크론 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (21-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Bacteroides tetaotaomicron bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 234.

다른 관점(21-iv)으로, 본 발명은 (a) 상기 서열번호 234의 염기 서열을 포함한 핵산 분자를 포함하는 박테로이데스 테타이오타오미크론 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 박테로이데스 테타이오타오미크론 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (21-iv), the present invention provides a composition comprising: (a) a composition comprising a nucleic acid probe for the detection of Bacteroides tetaiotamicron bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 234; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Bacteroides tetaotaomicron bacteria in a biological sample.

다른 관점(21-v)으로, 본 발명은 상기 서열번호 234의 염기 서열을 포함한 핵산 분자를 포함한 DNA 칩을 제공한다.In another aspect (21-v), the present invention provides a DNA chip comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 234.

다른 관점(21-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 234의 염기 서열을 포함한 핵산 분자를 포함하는 박테로이데스 테타이오타오미크론 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 박테로이데스 테타이오타오미크론 균을 검출 및 동정하는 방법을 제공한다.In another aspect (21-vi), the present invention relates to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) appropriate hybridization of the polynucleic acid of steps (a) and (b) with a composition comprising a nucleic acid probe for the detection of Bacteroides tetaotaomicron bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 234 And under washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying the strains present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Bacteroides tetaotaomicron bacteria in the biological sample.

다른 관점(22-i)으로, 본 발명은 클로스트리디움 디프실 균으로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (22-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from the Clostridium difficill bacillus:

GTTCGTCCGCCCCTG (C.diffc005, 서열번호 240).GTTCGTCCGCCCCTG (C.diffc005, SEQ ID NO: 240).

다른 관점(22-ii)으로, 본 발명은 상기 서열번호 240의 염기 서열을 포함한 핵산 분자를 포함하는 클로스트리디움 디프실 균의 검출용 핵산 탐침을 제공한다.In another aspect (22-ii), the present invention provides a nucleic acid probe for detecting Clostridium difficile bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 240.

다른 관점(22-iii)으로, 본 발명은 상기 서열번호 240의 염기 서열을 포함한 핵산 분자를 포함하는 클로스트리디움 디프실 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (22-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Clostridium difficile bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 240.

다른 관점(22-iv)으로, 본 발명은 (a) 상기 서열번호 240의 염기 서열을 포함한 핵산 분자를 포함하는 클로스트리디움 디프실 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 클로스트리디움 디프실 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (22-iv), the present invention provides a composition comprising (a) a nucleic acid probe for detecting Clostridium difficile bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 240; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Clostridium difficill bacteria in a biological sample.

다른 관점(22-v)으로, 본 발명은 상기 서열번호 240의 염기 서열을 포함한 핵산 분자를 포함하는 클로스트리디움 디프실 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (22-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting Clostridium difficile including a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 240 is fixed to a solid support.

다른 관점(22-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 240의 염기 서열을 포함한 핵산 분자를 포함하는 클로스트리디움 디프실 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 클로스트리디움 디프실 균을 검출 및 동정하는 방법을 제공한다.In another aspect (22-vi), the present invention provides a process for (a) isolating and / or concentrating a polynucleic acid, optionally present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) appropriate hybridization and washing conditions of the polynucleic acid of steps (a) and (b) with a composition comprising a nucleic acid probe for the detection of Clostridium diffyl bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 240; Contacting under; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Clostridium difficile bacteria in the biological sample.

다른 관점(23-i)으로, 본 발명은 해모필러스 아프로필라스 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (23-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following nucleotide sequences derived from 23S rRNA of Haemophilus apropylas:

GGTGAAGAACCCACT (H.aphro003, 서열번호 245).GGTGAAGAACCCACT (H.aphro003, SEQ ID NO: 245).

다른 관점으로, 본 발명은 해모필러스 아프로필라스 균의 ITS로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect, the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following nucleotide sequences derived from ITS of Haemophilus apropylas:

TGGGAGTGGGTTGTC (H.aphro001, 서열번호 246); 또는TGGGAGTGGGTTGTC (H.aphro001, SEQ ID NO: 246); or

TAACAAACCGGAAAC (H.aphro002, 서열번호 247).TAACAAACCGGAAAC (H.aphro002, SEQ ID NO: 247).

다른 관점(23-ii)으로, 본 발명은 상기 서열번호 245 내지 247중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 해모필러스 아프로필라스 균의 검출용 핵산 탐침을 제공한다.In another aspect (23-ii), the present invention provides a nucleic acid probe for detecting Haemophilus apropyl bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 245 to 247.

다른 관점(23-iii)으로, 본 발명은 상기 서열번호 245 내지 247중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 해모필러스 아프로필라스 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (23-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Haemophilus apropyl bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 245 to 247.

다른 관점(23-iv)으로, 본 발명은 (a) 상기 서열번호 245 내지 247중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 해모필러스 아프로필라스 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 해모필러스 아프로필라스 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (23-iv), the present invention provides a composition comprising: (a) a composition comprising a nucleic acid probe for detecting Haemophilus apropyl bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 245 to 247; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, to provide a kit for detecting and identifying Haemophilus apropyl bacteria in a biological sample.

다른 관점(23-v)으로, 본 발명은 상기 서열번호 245 내지 247중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 해모필러스 아프로필라스 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (23-v), the present invention provides a DNA in which a nucleic acid probe for detecting Haemophilus apropylas, including the nucleic acid molecule comprising any one of SEQ ID NOs: 245 to 247, is immobilized on a solid support. Provide chips.

다른 관점(23-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 245 내지 247중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 해모필러스 아프로필라스 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 해모필러스 아프로필라스 균을 검출 및 동정하는 방법을 제공한다.In another aspect (23-vi), the present invention is directed to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) a composition comprising a nucleic acid probe for detecting Haemophilus apropyl bacterium, wherein the polynucleic acid of steps (a) and (b) comprises a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 245 to 247 Contacting with appropriate hybridization and washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Haemophilus apropyl bacteria in the biological sample.

다른 관점(24-i-a)으로, 본 발명은 나이세리아 고노헤아 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (24-i-a), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from 23S rRNA of Neisseria gonohaea:

TATCAAAGTAGGGAT (N.gono005, 서열번호 254); 또는TATCAAAGTAGGGAT (N. gono005, SEQ ID NO: 254); or

AGTCAACGGGTAGGT (N.gono006, 서열번호 255).AGTCAACGGGTAGGT (N.gono006, SEQ ID NO: 255).

다른 관점(24-i-b)으로, 본 발명은 나이세리아 고노헤아 균의 ITS로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (24-i-b), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following nucleotide sequences derived from ITS of Neisseria gonohaea:

AACCTCTCGCAAGAG (N.gono002, 서열번호 256)AACCTCTCGCAAGAG (N.gono002, SEQ ID NO: 256)

다른 관점(24-ii)으로, 본 발명은 상기 서열번호 254 내지 256중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 나이세리아 고노헤아 균의 검출용 핵산 탐침을 제공한다.In another aspect (24-ii), the present invention provides a nucleic acid probe for detecting Neisseria gonohea bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 254 to 256.

다른 관점(24-iii)으로, 본 발명은 상기 서열번호 254 내지 256중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 나이세리아 고노헤아 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (24-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Neisseria gonohea bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 254 to 256.

다른 관점(24-iv)으로, 본 발명은 (a) 상기 서열번호 254 내지 256중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 나이세리아 고노헤아 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 나이세리아 고노헤아 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (24-iv), the present invention provides a composition comprising: (a) a composition comprising a nucleic acid probe for detecting Neisseria gonohea bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 254 to 256; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, to provide a kit for detecting and identifying Neisseria gonohea bacteria in a biological sample.

다른 관점(24-v)으로, 본 발명은 상기 서열번호 254 내지 256중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 나이세리아 고노헤아 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (24-v), the present invention provides a DNA chip in which a nucleic acid probe for detection of Neisseria gonohea bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 254 to 256 is fixed to a solid support. to provide.

다른 관점(24-Vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 254 내지 256중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 나이세리아 고노헤아 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 나이세리아 고노헤아 균을 검출 및 동정하는 방법을 제공한다.In another aspect (24-Vi), the present invention provides for (a) separating and / or concentrating polynucleic acid, optionally present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) the polynucleic acid of step (a) and (b) is suitable for a composition comprising a nucleic acid probe for detection of Neisseria gonohaea comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 254-256. Contacting under hybridization and washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Neisseria gonohea bacteria in the biological sample.

다른 관점(25-i-a)으로, 본 발명은 아이케넬라 코로덴스 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (25-i-a), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following nucleotide sequences derived from 23S rRNAs of E.ella corodens:

GGATAGGAGAAGGAA (E.corro005, 서열번호 262); 또는GGATAGGAGAAGGAA (E.corro005, SEQ ID NO: 262); or

ACTCATCATCGATCC (E.corro006, 서열번호 263).ACTCATCATCGATCC (E.corro006, SEQ ID NO: 263).

다른 관점(25-i-b)으로, 본 발명은 아이케넬라 코로덴스 균의 ITS로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (25-i-b), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following nucleotide sequences derived from the ITS of E.ella corodens:

AGTCGTAGAGCGGAG (E.corro001, 서열번호 264).AGTCGTAGAGCGGAG (E.corro001, SEQ ID NO: 264).

다른 관점(25-ii)으로, 본 발명은 상기 서열번호 262 내지 264중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 아이케넬라 코로덴스 균의 검출용 핵산 탐침을 제공한다.In another aspect (25-ii), the present invention provides a nucleic acid probe for detecting the bacteria of E.ella corodens comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 262 to 264.

다른 관점(25-iii)으로, 본 발명은 상기 서열번호 262 내지 264중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 아이케넬라 코로덴스 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (25-iii), the present invention provides a composition comprising a nucleic acid probe for the detection of E.ella corodens bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 262 to 264.

다른 관점(25-iv)으로, 본 발명은 (a) 상기 서열번호 262 내지 264중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 아이케넬라 코로덴스 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 아이케넬라 코로덴스 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (25-iv), the present invention provides a composition comprising: (a) a composition comprising a nucleic acid probe for detecting E.ella corodens bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 262 to 264; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying E.ella corodens bacteria in a biological sample.

다른 관점(25-v)으로, 본 발명은 상기 서열번호 262 내지 264중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 아이케넬라 코로덴스 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (25-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting E.ella corodens, comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 262 to 264, is immobilized on a solid support. To provide.

다른 관점(25-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 262 내지 264중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 아이케넬라 코로덴스 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 아이케넬라 코로덴스 균을 검출 및 동정하는 방법을 제공한다.In another aspect (25-vi), the present invention provides a method for (a) isolating and / or concentrating polynucleic acid, optionally present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) a composition comprising a nucleic acid probe for detecting E.ella corodens bacteria comprising the nucleic acid molecule of any one of SEQ ID NOs: 262 to 264, using the polynucleic acid of steps (a) and (b); Contacting under appropriate hybridization and washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying E.ella corodens in the biological sample.

다른 관점(26-i)으로, 본 발명은 박테로이데스 불가투스 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (26-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following nucleotide sequences derived from the Bacteroides buccus 23S rRNA:

AGTCAGCGTCGAAGG (b.vulga03, 서열번호 268); 또는AGTCAGCGTCGAAGG (b.vulga03, SEQ ID NO: 268); or

CGAATGCGCATCAGT (b.vulga07, 서열번호 269).CGAATGCGCATCAGT (b.vulga07, SEQ ID NO: 269).

다른 관점(26-ii)으로, 본 발명은 상기 서열번호 268 또는 269의 염기 서열을 포함한 핵산 분자를 포함하는 박테로이데스 불가투스 균의 검출용 핵산 탐침을 제공한다.In another aspect (26-ii), the present invention provides a nucleic acid probe for detecting Bacteroides vulgaris comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 268 or 269.

다른 관점(26-iii)으로, 본 발명은 상기 서열번호 268 또는 269의 염기 서열을 포함한 핵산 분자를 포함하는 박테로이데스 불가투스 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (26-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Bacteroides vulgaris comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 268 or 269.

다른 관점(26-iv)으로, 본 발명은 (a) 상기 서열번호 268 또는 269의 염기 서열을 포함한 핵산 분자를 포함하는 박테로이데스 불가투스 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 박테로이데스 불가투스 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (26-iv), the present invention provides a composition comprising: (a) a composition comprising a nucleic acid probe for detecting Bacteroides vulgaris comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 268 or 269; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Bacteroides vulgaris in a biological sample.

다른 관점(26-v)으로, 본 발명은 상기 서열번호 268 또는 269의 염기 서열을 포함한 핵산 분자를 포함하는 박테로이데스 불가투스 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (26-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting Bacteroides buccus bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 268 or 269 is fixed to a solid support. .

다른 관점(26-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 268 또는 269중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 박테로이데스 불가투스 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 박테로이데스 불가투스 균을 검출 및 동정하는 방법을 제공한다.In another aspect (26-vi), the present invention provides a method for (a) isolating and / or concentrating polynucleic acid, optionally present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) a composition comprising a nucleic acid probe for the detection of Bacteroides buccus bacteria comprising the nucleic acid molecule of any one of SEQ ID NO: 268 or 269 in the polynucleic acid of step (a) and (b); Contacting under appropriate hybridization and washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Bacteroides vulgaris in the biological sample.

다른 관점(27-i)으로, 본 발명은 브란하멜라 카타르할리스 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (27-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from 23S rRNA of Branhamela catarrhalis fungus:

ATATCTTCGCGCTGT (B.catar005, 서열번호 280).ATATCTTCGCGCTGT (B.catar005, SEQ ID NO: 280).

다른 관점(27-ii)으로, 본 발명은 상기 서열번호 280의 염기 서열을 포함한 핵산 분자를 포함하는 브란하멜라 카타르할리스 균의 검출용 핵산 탐침을 제공한다.In another aspect (27-ii), the present invention provides a nucleic acid probe for detection of Branhamella catarrhalis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 280.

다른 관점(27-iii)으로, 본 발명은 상기 서열번호 280의 염기 서열을 포함한 핵산 분자를 포함하는 브란하멜라 카타르할리스 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (27-iii), the present invention provides a composition comprising a nucleic acid probe for detection of Branhamella catarrhalis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 280.

다른 관점(27-iv)으로, 본 발명은 (a) 상기 서열번호 280의 염기 서열을 포함한 핵산 분자를 포함하는 브란하멜라 카타르할리스 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 브란하멜라 카타르할리스 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (27-iv), the present invention provides a composition comprising (a) a nucleic acid probe for detection of Branhamella catarrhalis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 280; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Branhamella catarrhalis bacteria in a biological sample.

다른 관점(27-v)으로, 본 발명은 상기 서열번호 280의 염기 서열을 포함한 핵산 분자를 포함하는 브란하멜라 카타르할리스 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (27-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting Branhamella catarrhalis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 280 is fixed to a solid support.

다른 관점(27-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 280의 염기 서열을 포함한 핵산 분자를 포함하는 브란하멜라 카타르할리스 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 브란하멜라 카타르할리스 균을 검출 및 동정하는 방법을 제공한다.In another aspect (27-vi), the present invention provides a process for (a) isolating and / or concentrating polynucleic acid, optionally present in a biological sample, and (b) optionally amplifying the polynucleic acid with appropriate forward and reverse primer pairs; (c) appropriate hybridization and washing conditions with a composition comprising a nucleic acid probe for detecting Branhamella catarrhalis comprising the nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 280 in the polynucleic acid of steps (a) and (b) Contacting under; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Branhamella catarrhalis bacteria in the biological sample.

다른 관점(28-i)으로, 본 발명은 수테렐라 와즈워텐시스 균의 ITS로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다;In another aspect 28-i, the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following nucleotide sequences derived from the ITS of Suterella wazworthensis;

TTCGGGTCCGTAATT (Swad02, 서열번호 292);TTCGGGTCCGTAATT (Swad02, SEQ ID NO: 292);

AATCAAGGCCGAGGC (Swad03, 서열번호 293); 또는AATCAAGGCCGAGGC (Swad03, SEQ ID NO: 293); or

GCCGAGGCGTGATGA (Swad04, 서열번호 294).GCCGAGGCGTGATGA (Swad04, SEQ ID NO: 294).

다른 관점(28-ii)으로, 본 발명은 상기 서열번호 292 내지 294중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 수테렐라 와즈워텐시스 균의 검출용 핵산 탐침을 제공한다.In another aspect (28-ii), the present invention provides a nucleic acid probe for detecting Suterella wazworthensis comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 292 to 294.

다른 관점(28-iii)으로, 본 발명은 상기 서열번호 292 내지 294중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 수테렐라 와즈워텐시스 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (28-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Suterella wazworthensis comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 292 to 294.

다른 관점(28-iv)으로, 본 발명은 (a) 상기 서열번호 292 내지 294중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 수테렐라 와즈워텐시스균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 수테렐라 와즈워텐시스 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (28-iv), the present invention provides a composition comprising: (a) a composition comprising a nucleic acid probe for detecting Suterella wazworthissis comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 292 to 294; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Suterella wazworthensis bacteria in a biological sample.

다른 관점(28-v)으로, 본 발명은 상기 서열번호 292 내지 294중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 수테렐라 와즈워텐시스 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (28-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting Suterella wazworthensis comprising a nucleic acid molecule comprising any one of the nucleotide sequences of SEQ ID NOs: 292 to 294 is immobilized on a solid support. To provide.

다른 관점(28-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 292 내지 294중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 수테렐라 와즈워텐시스 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 수테렐라 와즈워텐시스 균을 검출 및 동정하는 방법을 제공한다.In another aspect (28-vi), the present invention provides a method for (a) isolating and / or concentrating polynucleic acid, optionally present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) a composition comprising a nucleic acid probe for detecting Suterella wazworthensis, the polynucleic acid of steps (a) and (b) comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 292 to 294; Contacting under appropriate hybridization and washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Suterella wazworthis bacteria in the biological sample.

다른 관점(29-i)으로, 본 발명은 엑티노마이세스 이스라엘이 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (29-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from the bacterium 23S rRNA of Actinomyces Israel:

AACCTGGCTGGTGGC (Aciil, 서열번호 296).AACCTGGCTGGTGGC (Aciil, SEQ ID NO: 296).

다른 관점(29-ii)으로, 본 발명은 상기 서열번호 296의 염기 서열을 포함한 핵산 분자를 포함하는 엑티노마이세스 이스라엘이 균의 검출용 핵산 탐침을 제공한다.In another aspect (29-ii), the present invention provides a nucleic acid probe for detecting an bacterium of Extinomyse Israel comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 296.

다른 관점(29-iii)으로, 본 발명은 상기 서열번호 296의 염기 서열을 포함한 핵산 분자를 포함하는 엑티노마이세스 이스라엘이 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (29-iii), the present invention provides a composition comprising a nucleic acid probe for detecting an bacterium of Extinomyse Israel comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 296.

다른 관점(29-iv)으로, 본 발명은 (a) 상기 서열번호 296의 염기 서열을 포함한 핵산 분자를 포함하는 엑티노마이세스 이스라엘이 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 엑티노마이세스 이스라엘이 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (29-iv), the present invention provides a composition comprising: (a) a composition comprising a nucleic acid probe for detecting an bacterium of Extinomyse Israel comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 296; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying bacteria by actinomyces Israel in biological samples.

다른 관점(29-v)으로, 본 발명은 상기 서열번호 296의 염기 서열을 포함한 핵산 분자를 포함하는 엑티노마이세스 이스라엘이 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (29-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting an actinomyces Israeli bacterium comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 296 is fixed to a solid support.

다른 관점(29-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 296의 염기 서열을 포함한 핵산 분자를 포함하는 엑티노마이세스 이스라엘이 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 엑티노마이세스 이스라엘이 균을 검출 및 동정하는 방법을 제공한다.In another aspect (29-vi), the present invention provides a process for (a) isolating and / or concentrating polynucleic acid, optionally present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) appropriate hybridization and washing of the polynucleic acid of steps (a) and (b) with a composition comprising a nucleic acid probe for detecting the bacterium Extinomyse Israel comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 296 Contacting under conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying bacteria in said biological sample.

다른 관점(30-i)으로, 본 발명은 스타필로코코스 에피더미디스 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (30-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from the 23S rRNA of Staphylococcus epidermidis bacteria:

GATAGATAACAGGTG (SeM01, 서열번호 299); 또는GATAGATAACAGGTG (SeM01, SEQ ID NO: 299); or

AGGGTTCACGCCCAG (SeM02, 서열번호 300).AGGGTTCACGCCCAG (SeM02, SEQ ID NO: 300).

다른 관점(30-ii)으로, 본 발명은 상기 서열번호 299 또는 300의 염기 서열을 포함한 핵산 분자를 포함하는 스타필로코코스 에피더미디스 균의 검출용 핵산 탐침을 제공한다.In another aspect (30-ii), the present invention provides a nucleic acid probe for detecting Staphylococcus epidermidis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 299 or 300.

다른 관점(30-iii)으로, 본 발명은 상기 서열번호 299 또는 300의 염기 서열을 포함한 핵산 분자를 포함하는 스타필로코코스 에피더미디스 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (30-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Staphylococcus epidermidis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 299 or 300.

다른 관점(30-iv)으로, 본 발명은 (a) 상기 서열번호 299 또는 300의 염기 서열을 포함한 핵산 분자를 포함하는 스타필로코코스 에피더미디스 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 스타필로코코스 에피더미디스 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (30-iv), the present invention (a) composition comprising a nucleic acid probe for the detection of Staphylococcus epidermidis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 299 or 300; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, to provide a kit for detecting and identifying Staphylococcus epidermidis bacteria in a biological sample.

다른 관점(30-v)으로, 본 발명은 상기 서열번호 299 또는 300의 염기 서열을 포함한 핵산 분자를 포함하는 스타필로코코스 에피더미디스 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (30-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting Staphylococcus epidermidis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 299 or 300 is fixed to a solid support. do.

다른 관점(30-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 299 또는 300의 염기 서열을 포함한 핵산 분자를 포함하는 스타필로코코스 에피더미디스 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 스타필로코코스 에피더미디스 균을 검출 및 동정하는 방법을 제공한다.In another aspect (30-vi), the present invention is directed to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) appropriate hybridization of the polynucleic acid of steps (a) and (b) with a composition comprising a nucleic acid probe for detecting Staphylococcus epidermidis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 299 or 300 And under washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Staphylococcus epidermidis bacteria in the biological sample.

다른 관점(31-i)으로, 본 발명은 벌코데리아 세파시아 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (31-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following nucleotide sequences derived from 23S rRNAs of the bacterium Sephacia:

TTGTTAGCCGAACGC (Bur23, 서열번호 304); 또는TTGTTAGCCGAACGC (Bur23, SEQ ID NO: 304); or

GGGTGTGGCGCGAGC (Bur01, 서열번호 305).GGGTGTGGCGCGAGC (Bur01, SEQ ID NO: 305).

다른 관점(31-ii)으로, 본 발명은 상기 서열번호 304 또는 305의 염기 서열을 포함한 핵산 분자를 포함하는 벌코데리아 세파시아 균의 검출용 핵산 탐침을 제공한다.In another aspect (31-ii), the present invention provides a nucleic acid probe for the detection of Bulcoderia cephacia bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 304 or 305.

다른 관점(31-iii)으로, 본 발명은 상기 서열번호 304 또는 305의 염기 서열을 포함한 핵산 분자를 포함하는 벌코데리아 세파시아 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (31-iii), the present invention provides a composition comprising a nucleic acid probe for the detection of Bulcoderia cephacia bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 304 or 305.

다른 관점(31-iv)으로, 본 발명은 (a) 상기 서열번호 304 또는 305의 염기 서열을 포함한 핵산 분자를 포함하는 벌코데리아 세파시아균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 벌코데리아 세파시아 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (31-iv), the present invention provides a composition comprising (a) a nucleic acid probe for the detection of Bulcoderia sefacia bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 304 or 305; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Bulcoderia cephacia bacteria in a biological sample.

다른 관점(31-v)으로, 본 발명은 상기 서열번호 304 또는 305의 염기 서열을 포함한 핵산 분자를 포함하는 벌코데리아 세파시아 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (31-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting a Bulcoderia cephacia comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 304 or 305 is fixed to a solid support. .

다른 관점(31-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 304 또는 305의 염기 서열을 포함한 핵산 분자를 포함하는 벌코데리아 세파시아 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 벌코데리아 세파시아 균을 검출 및 동정하는 방법을 제공한다.In another aspect (31-vi), the present invention provides a method for (a) isolating and / or concentrating polynucleic acid, optionally present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) appropriate hybridization of the polynucleic acid of steps (a) and (b) with a composition comprising a nucleic acid probe for the detection of Bulcoderia cephacia bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 304 or 305; and Contacting under washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Bulcoderia cephacia bacteria in the biological sample.

다른 관점(32-i)으로, 본 발명은 살모넬라 엔테리티디스 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (32-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from Salmonella enteritidis bacteria 23S rRNA:

GCCTGAATCAGCATG (Styp23, 서열번호 307).GCCTGAATCAGCATG (Styp23, SEQ ID NO: 307).

다른 관점(32-ii)으로, 본 발명은 상기 서열번호 307의 염기 서열을 포함한 핵산 분자를 포함하는 살모넬라 엔테리티디스 균의 검출용 핵산 탐침을 제공한다.In another aspect (32-ii), the present invention provides a nucleic acid probe for the detection of Salmonella enteritidis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 307.

다른 관점(32-iii)으로, 본 발명은 상기 서열번호 307의 염기 서열을 포함한 핵산 분자를 포함하는 살모넬라 엔테리티디스 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (32-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Salmonella enteritidis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 307.

다른 관점(32-iv)으로, 본 발명은 (a) 상기 서열번호 307의 염기 서열을 포함한 핵산 분자를 포함하는 살모넬라 엔테리티디스 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 살모넬라 엔테리티디스 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (32-iv), the present invention provides a composition comprising a nucleic acid probe for detecting Salmonella enteritidis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 307; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Salmonella enteritidis bacteria in a biological sample.

다른 관점(32-v)으로, 본 발명은 상기 서열번호 307의 염기 서열을 포함한 핵산 분자를 포함하는 살모넬라 엔테리티디스 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (32-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting Salmonella enteritidis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 307 is fixed to a solid support.

다른 관점(32-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 307의 염기 서열을 포함한 핵산 분자를 포함하는 살모넬라 엔테리티디스 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 살모넬라 엔테리티디스 균을 검출 및 동정하는 방법을 제공한다.In another aspect (32-vi), the present invention provides a method of (a) isolating and / or concentrating polynucleic acid, optionally present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) the polynucleic acid of steps (a) and (b) under appropriate hybridization and washing conditions with a composition comprising a nucleic acid probe for the detection of Salmonella enteritidis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 307; Contact; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Salmonella enteritidis bacteria in the biological sample.

다른 관점(33-i)으로, 본 발명은 에스케리치아 콜라이 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다.In another aspect (33-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from 23S rRNA of Escherichia coli.

CTGAAGCGACAAATG (E coli 003, 서열번호 312).CTGAAGCGACAAATG (E coli 003, SEQ ID NO: 312).

다른 관점(33-ii)으로, 본 발명은 상기 서열번호 312의 염기 서열을 포함한 핵산 분자를 포함하는 에스케리치아 콜라이 균의 검출용 핵산 탐침을 제공한다.In another aspect (33-ii), the present invention provides a nucleic acid probe for detecting Escherichia coli, including a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 312.

다른 관점(33-iii)으로, 본 발명은 상기 서열번호 312의 염기 서열을 포함한 핵산 분자를 포함하는 에스케리치아 콜라이 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (33-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Escherichia coli, including a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 312.

다른 관점(33-iv)으로, 본 발명은 (a) 상기 서열번호 312의 염기 서열을 포함한 핵산 분자를 포함하는 에스케리치아 콜라이 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 에스케리치아 콜라이 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (33-iv), the present invention provides a composition comprising a nucleic acid probe for detecting Escherichia coli, comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 312; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Escherichia coli bacteria in a biological sample.

다른 관점(33-v)으로, 본 발명은 상기 서열번호 312의 염기 서열을 포함한 핵산 분자를 포함하는 에스케리치아 콜라이 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (33-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting Escherichia coli comprising the nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 312 is fixed to a solid support.

다른 관점(33-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 312의 염기 서열을 포함한 핵산 분자를 포함하는 에스케리치아 콜라이 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 에스케리치아 콜라이 균을 검출 및 동정하는 방법을 제공한다.In another aspect (33-vi), the present invention provides a method for (a) isolating and / or concentrating polynucleic acid, optionally present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) the polynucleic acid of steps (a) and (b) under appropriate hybridization and washing conditions with a composition comprising a nucleic acid probe for detection of Escherichia coli comprising the nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 312. Contact; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Escherichia coli bacteria in the biological sample.

다른 관점(34-i)으로, 본 발명은 크레브시엘라 뉴모니에 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect 34-i, the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following nucleotide sequences derived from the 23S rRNA of the Crevciella pneumoniae bacterium:

GTACACCAAAATGCA (Kpneu 23, 서열번호 317); 또는GTACACCAAAATGCA (Kpneu 23, SEQ ID NO: 317); or

GCTGAGACCAGTCGA (K.pneu002, 서열번호 318).GCTGAGACCAGTCGA (K.pneu002, SEQ ID NO: 318).

다른 관점(34-ii)으로, 본 발명은 상기 서열번호 317 또는 318의 염기 서열을 포함한 핵산 분자를 포함하는 크레브시엘라 뉴모니에 균의 검출용 핵산 탐침을 제공한다.In another aspect (34-ii), the present invention provides a nucleic acid probe for detecting Crebesciella pneumoniae comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 317 or 318.

다른 관점(34-iii)으로, 본 발명은 상기 서열번호 317 또는 318의 염기 서열을 포함한 핵산 분자를 포함하는 크레브시엘라 뉴모니에 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (34-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Crebesciella pneumoniae comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 317 or 318.

다른 관점(34-iv)으로, 본 발명은 (a) 상기 서열번호 317 또는 318의 염기 서열을 포함한 핵산 분자를 포함하는 크레브시엘라 뉴모니에 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 크레브시엘라 뉴모니에 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (34-iv), the present invention provides a composition comprising (a) a nucleic acid probe for detecting Crebesciella pneumoniae comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 317 or 318; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying CrebSiela pneumoniae in a biological sample.

다른 관점(34-v)으로, 본 발명은 상기 서열번호 317 내지 318중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 크레브시엘라 뉴모니에 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (34-v), the present invention provides a nucleic acid probe for detecting Crebesciella pneumoniae comprising a nucleic acid molecule of any one of SEQ ID NOs: 317 to 318 to a solid support. Provide a DNA chip.

다른 관점(34-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 317 또는 318의 염기 서열을 포함한 핵산 분자를 포함하는 크레브시엘라 뉴모니에 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 크레브시엘라 뉴모니에 균을 검출 및 동정하는 방법을 제공한다.In another aspect 34-vi, the present invention is directed to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) the polynucleic acid of step (a) and (b) is suitable for a composition comprising a nucleic acid probe for the detection of the Crebessiella pneumoniae comprising the nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 317 or 318. Contacting under hybridization and washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying the strains present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying the Crebeciella pneumoniae bacteria in the biological sample.

다른 관점(35-i)으로, 본 발명은 프로테우스 미라빌리스 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (35-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following nucleotide sequences derived from 23S rRNA of Proteus mirabilis:

GTTACCAACAATCGT (Pm, 서열번호 321);GTTACCAACAATCGT (Pm, SEQ ID NO: 321);

GGCGACGGTCGTCCC (Pm002, 서열번호 322);GGCGACGGTCGTCCC (Pm002, SEQ ID NO: 322);

GATGACGAACCACCA (Pm003, 서열번호 323); 또는GATGACGAACCACCA (Pm003, SEQ ID NO: 323); or

TGAAGCAATTGATGC (Pm004, 서열번호 324).TGAAGCAATTGATGC (Pm004, SEQ ID NO: 324).

다른 관점(35-ii)으로, 본 발명은 상기 서열번호 321 내지 324중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 프로테우스 미라빌리스 균의 검출용 핵산 탐침을 제공한다.In another aspect (35-ii), the present invention provides a nucleic acid probe for detecting Proteus mirabilis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 321 to 324.

다른 관점(35-iii)으로, 본 발명은 상기 서열번호 321 내지 324중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 프로테우스 미라빌리스 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (35-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Proteus mirabilis, which comprises a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 321 to 324.

다른 관점(35-iv)으로, 본 발명은 (a) 상기 서열번호 321 내지 324중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 프로테우스 미라빌리스 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 프로테우스 미라빌리스 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (35-iv), the present invention provides a composition comprising (a) a nucleic acid probe for detecting Proteus mirabilis comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 321 to 324; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Proteus mirabilis bacteria in a biological sample.

다른 관점(35-v)으로, 본 발명은 상기 서열번호 321 내지 324중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 프로테우스 미라빌리스 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (35-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting Proteus mirabilis, including a nucleic acid molecule comprising any one of the nucleotide sequences of SEQ ID NOs: 321 to 324, is immobilized on a solid support. to provide.

다른 관점(35-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 321 내지 324중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 프로테우스 미라빌리스 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 프로테우스 미라빌리스 균을 검출 및 동정하는 방법을 제공한다.In another aspect (35-vi), the present invention provides a method for (a) isolating and / or concentrating a polynucleic acid, optionally present in a biological sample, and (b) optionally amplifying the polynucleic acid with an appropriate forward and reverse primer pair; (c) the polynucleic acid of step (a) and (b) is suitable for a composition comprising a nucleic acid probe for detecting Proteus mirabilis, comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 321 to 324. Contacting under hybridization and washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Proteus mirabilis bacteria in the biological sample.

다른 관점(36-i)으로, 본 발명은 스트렙토코코스 뉴모니에 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect 36-i, the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following nucleotide sequence derived from Streptococcus pneumoniae 23S rRNA:

TAGGACTGCAATGTG (StreppM, 서열번호 328).TAGGACTGCAATGTG (StreppM, SEQ ID NO: 328).

다른 관점(36-ii)으로, 본 발명은 상기 서열번호 328의 염기 서열을 포함한 핵산 분자를 포함하는 스트렙토코코스 뉴모니에 균의 검출용 핵산 탐침을 제공한다.In another aspect (36-ii), the present invention provides a nucleic acid probe for detecting Streptococcus pneumoniae comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 328.

다른 관점(36-iii)으로, 본 발명은 상기 서열번호 328의 염기 서열을 포함한 핵산 분자를 포함하는 스트렙토코코스 뉴모니에 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (36-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Streptococcus pneumoniae comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 328.

다른 관점(36-iv)으로, 본 발명은 (a) 상기 서열번호 328의 염기 서열을 포함한 핵산 분자를 포함하는 스트렙토코코스 뉴모니에 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 스트렙토코코스 뉴모니에 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (36-iv), the present invention provides a composition comprising (a) a nucleic acid probe for detecting Streptococcus pneumoniae comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 328; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Streptococcus pneumoniae bacteria in a biological sample.

다른 관점(36-v)으로, 본 발명은 상기 서열번호 328의 염기 서열을 포함한 핵산 분자를 포함하는 스트렙토코코스 뉴모니에 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (36-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting Streptococcus pneumoniae comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 328 is fixed to a solid support.

다른 관점(36-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 328의 염기 서열을 포함한 핵산 분자를 포함하는 스트렙토코코스 뉴모니에 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 스트렙토코코스 뉴모니에 균을 검출 및 동정하는 방법을 제공한다.In another aspect 36-vi, the present invention is directed to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) appropriate hybridization and washing conditions with a composition comprising a nucleic acid probe for detecting Streptococcus pneumoniae comprising the polynucleic acid of step (a) and (b) comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 328 Contacting under; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Streptococcus pneumoniae bacteria in the biological sample.

다른 관점(37-i)으로, 본 발명은 비브리오 벌른피커스 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (37-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from the 23S rRNA of Vibrio Bernique Picus:

GTTGACGATGCATGT (Vvul02, 서열번호 333).GTTGACGATGCATGT (Vvul02, SEQ ID NO: 333).

다른 관점(37-ii)으로, 본 발명은 상기 서열번호 333의 염기 서열을 포함한 핵산 분자를 포함하는 비브리오 벌른피커스 균의 검출용 핵산 탐침을 제공한다.In another aspect (37-ii), the present invention provides a nucleic acid probe for the detection of Vibrio Bernickus bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 333.

다른 관점(37-iii)으로, 본 발명은 상기 서열번호 333의 염기 서열을 포함한 핵산 분자를 포함하는 비브리오 벌른피커스 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (37-iii), the present invention provides a composition comprising a nucleic acid probe for the detection of Vibrio Bernique Picus bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 333.

다른 관점(37-iv)으로, 본 발명은 (a) 상기 서열번호 333의 염기 서열을 포함한 핵산 분자를 포함하는 비브리오 벌른피커스 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 비브리오 벌른피커스 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (37-iv), the present invention provides a composition comprising (a) a nucleic acid probe for detecting Vibrio Bernoulcus bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 333; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Vibrio Bernoullican bacteria in a biological sample.

다른 관점(37-v)으로, 본 발명은 상기 서열번호 333의 염기 서열을 포함한 핵산 분자를 포함하는 비브리오 벌른피커스 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (37-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting a Vibrio Bernique Picus bacterium comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 333 is fixed to a solid support.

다른 관점(37-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 333의 염기 서열을 포함한 핵산 분자를 포함하는 비브리오 벌른피커스 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 비브리오 벌른피커스 균을 검출 및 동정하는 방법을 제공한다.In another aspect (37-vi), the present invention is directed to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) the polynucleic acid of steps (a) and (b) under appropriate hybridization and washing conditions with a composition comprising a nucleic acid probe for the detection of Vibrio Bernique Picus bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 333. Contact; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Vibrio Bernoullican bacteria in the biological sample.

다른 관점(38-i)으로, 본 발명은 슈도모나스 애루지노사 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect 38-i, the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from Pseudomonas aeruginosa 23S rRNA:

GAAGTGCCGAGCATG (P.aeru001, 서열번호 339); 또는GAAGTGCCGAGCATG (P.aeru001, SEQ ID NO: 339); or

GGATCTTTGAAGTGA (Pa03, 서열번호 340).GGATCTTTGAAGTGA (Pa03, SEQ ID NO: 340).

다른 관점(38-ii)으로, 본 발명은 상기 서열번호 339 또는 340의 염기 서열을 포함한 핵산 분자를 포함하는 슈도모나스 애루지노사 균의 검출용 핵산 탐침을 제공한다.In another aspect (38-ii), the present invention provides a nucleic acid probe for detecting Pseudomonas aeruginosa bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 339 or 340.

다른 관점(38-iii)으로, 본 발명은 상기 서열번호 339 또는 340의 염기 서열을 포함한 핵산 분자를 포함하는 슈도모나스 애루지노사 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (38-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Pseudomonas aeruginosa bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 339 or 340.

다른 관점(38-iv)으로, 본 발명은 (a) 상기 서열번호 339 또는 340의 염기 서열을 포함한 핵산 분자를 포함하는 슈도모나스 애루지노사 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 슈도모나스 애루지노사 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (38-iv), the present invention provides a composition comprising a nucleic acid probe for detecting Pseudomonas aeruginosa bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 339 or 340; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Pseudomonas aeruginosa bacteria in a biological sample.

다른 관점(38-v)으로, 본 발명은 상기 서열번호 339 또는 340의 염기 서열을 포함한 핵산 분자를 포함하는 슈도모나스 애루지노사 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (38-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting Pseudomonas aeruginosa comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 339 or 340 is fixed to a solid support.

다른 관점(38-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 339 또는 340의 염기 서열을 포함한 핵산 분자를 포함하는 슈도모나스 애루지노사 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 슈도모나스 애루지노사 균을 검출 및 동정하는 방법을 제공한다.In another aspect 38-vi, the present invention provides a method for the preparation of (a) isolating and / or concentrating polynucleic acid, optionally present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) appropriate hybridization and washing of the polynucleic acid of steps (a) and (b) with a composition comprising a nucleic acid probe for the detection of Pseudomonas aeruginosa bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 339 or 340 Contacting under conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Pseudomonas aeruginosa bacteria in the biological sample.

다른 관점(39-i)으로, 본 발명은 에로모나스 하이드로필라 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect 39-i, the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following nucleotide sequence derived from the 23S rRNA of Eromonas hydrophila:

GGCGCCTCGGTAGGG (Ah, 서열번호 347).GGCGCCTCGGTAGGG (Ah, SEQ ID NO: 347).

다른 관점(39-ii)으로, 본 발명은 상기 서열번호 347의 염기 서열을 포함한 핵산 분자를 포함하는 에로모나스 하이드로필라 균의 검출용 핵산 탐침을 제공한다.In another aspect (39-ii), the present invention provides a nucleic acid probe for the detection of E. hydronas bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 347.

다른 관점(39-iii)으로, 본 발명은 상기 서열번호 347의 염기 서열을 포함한 핵산 분자를 포함하는 에로모나스 하이드로필라 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (39-iii), the present invention provides a composition comprising a nucleic acid probe for the detection of E. hydronas bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 347.

다른 관점(39-iv)으로, 본 발명은 (a) 상기 서열번호 347의 염기 서열을 포함한 핵산 분자를 포함하는 에로모나스 하이드로필라 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 에로모나스 하이드로필라 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (39-iv), the present invention provides a composition comprising (a) a nucleic acid probe for the detection of E. hydronas bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 347; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying the E. hydromonas bacteria in a biological sample.

다른 관점(39-v)으로, 본 발명은 상기 서열번호 347의 염기 서열을 포함한 핵산 분자를 포함하는 에로모나스 하이드로필라 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (39-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting an E. monella hydrophilic bacterium comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 347 is fixed to a solid support.

다른 관점(39-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이며 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 347의 염기 서열을 포함한 핵산 분자를 포함하는 에로모나스 하이드로필라 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 에로모나스 하이드로필라 균을 검출 및 동정하는 방법을 제공한다.In another aspect 39-vi, the present invention provides a method for (a) isolating and / or concentrating polynucleic acid, optionally present in a biological sample, and (b) amplifying the polynucleic acid in an appropriate ; (c) the polynucleic acid of steps (a) and (b) under appropriate hybridization and washing conditions with a composition comprising a nucleic acid probe for the detection of Eromonas hydrophila comprising the nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 347. Contact; (d) detecting the hybrid formed in step (c); (e) identifying the strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying the E. hydronas bacteria in the biological sample.

다른 관점(40-i)으로, 본 발명은 리스테리아 모노사이토제네스 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (40-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from 23S rRNA of Listeria monocytogenes fungus:

GGGTGCAAGCCCGAG (LM, 서열번호 354).GGGTGCAAGCCCGAG (LM, SEQ ID NO: 354).

다른 관점(40-ii)으로, 본 발명은 상기 서열번호 354의 염기 서열을 포함한 핵산 분자를 포함하는 리스테리아 모노사이토제네스 균의 검출용 핵산 탐침을 제공한다.In another aspect (40-ii), the present invention provides a nucleic acid probe for detecting Listeria monocytogenes bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 354.

다른 관점(40-iii)으로, 본 발명은 상기 서열번호 354의 염기 서열을 포함한 핵산 분자를 포함하는 리스테리아 모노사이토제네스 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (40-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Listeria monocytogenes bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 354.

다른 관점(40-iv)으로, 본 발명은 (a) 상기 서열번호 354의 염기 서열을 포함한 핵산 분자를 포함하는 리스테리아 모노사이토제네스 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 리스테리아 모노사이토제네스 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (40-iv), the present invention provides a composition comprising (a) a nucleic acid probe for detecting Listeria monocytogenes bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 354; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Listeria monocytogenes bacteria in a biological sample.

다른 관점(40-v)으로, 본 발명은 상기 서열번호 354의 염기 서열을 포함한 핵산 분자를 포함하는 리스테리아 모노사이토제네스 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (40-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting Listeria monocytogenes bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 354 is fixed to a solid support.

다른 관점(40-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 354의 염기 서열을 포함한 핵산 분자를 포함하는 리스테리아 모노사이토제네스 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 리스테리아 모노사이토제네스 균을 검출 및 동정하는 방법을 제공한다.In another aspect (40-vi), the present invention provides a method for (a) isolating and / or concentrating polynucleic acid, optionally present in a biological sample, and (b) optionally amplifying the polynucleic acid with appropriate forward and reverse primer pairs; (c) the polynucleic acid of steps (a) and (b) under appropriate hybridization and washing conditions with a composition comprising a nucleic acid probe for detecting Listeria monocytogenes bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 354. Contact; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Listeria monocytogenes bacteria in the biological sample.

다른 관점(41-i)으로, 본 발명은 엔테로코코스 페슘 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect (41-i), the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following nucleotide sequences derived from the 23S rRNA of Enterococcus pesium bacterium:

TTACGATTGTGTGAA (E.faecium002, 서열번호 359); 또는TTACGATTGTGTGAA (E.faecium002, SEQ ID NO: 359); or

ATAGCACATTCGAGG (E.faecium003, 서열번호 360).ATAGCACATTCGAGG (E.faecium003, SEQ ID NO: 360).

다른 관점(41-ii)으로, 본 발명은 상기 서열번호 359 또는 360의 염기 서열을 포함한 핵산 분자를 포함하는 엔테로코코스 페슘 균의 검출용 핵산 탐침을 제공한다.In another aspect (41-ii), the present invention provides a nucleic acid probe for detecting enterococcus pesium bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 359 or 360.

다른 관점(41-iii)으로, 본 발명은 상기 서열번호 359 또는 360의 염기 서열을 포함한 핵산 분자를 포함하는 엔테로코코스 폐습 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (41-iii), the present invention provides a composition comprising a nucleic acid probe for detecting enterococcus bacterium comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 359 or 360.

다른 관점(41-iv)으로, 본 발명은 (a) 상기 서열번호 359 또는 360의 염기 서열을 포함한 핵산 분자를 포함하는 엔테로코코스 페슘 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 엔테로코코스 페슘 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (41-iv), the present invention provides a composition comprising (a) a nucleic acid probe for detecting Enterococcus pesium bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 359 or 360; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying enterococcus bacterium in a biological sample.

다른 관점(41-v)으로, 본 발명은 상기 서열번호 359 또는 360의 염기 서열을 포함한 핵산 분자를 포함하는 엔테로코코스 페슘 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (41-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting enterococcus bacterium comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 359 or 360 is fixed to a solid support.

다른 관점(41-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 359 또는 360중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 엔테로코코스 페슘 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 엔테로코코스 페슘 균을 검출 및 동정하는 방법을 제공한다.In another aspect (41-vi), the present invention provides a method for (a) isolating and / or enriching polynucleic acid, optionally present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) appropriate hybridization of the polynucleic acid of steps (a) and (b) with a composition comprising a nucleic acid probe for the detection of Enterococcus pesium bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 359 or 360 And under washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Enterococcus pesium bacteria in the biological sample.

다른 관점(42-i)으로, 본 발명은 스타필로코코스 아우레우스 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect 42-i, the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from 23S rRNA of Staphylococcus aureus fungus:

GATTGCACGTCTAAG (S.aureus004, 서열번호 365);GATTGCACGTCTAAG (S.aureus 004, SEQ ID NO: 365);

AATCCGGTACTCGTT (S.aureus005, 서열번호 366); 또는AATCCGGTACTCGTT (S.aureus 005, SEQ ID NO: 366); or

TCTTCGAGTCGTTGA (Saure03(Saureus03), 서열번호 367).TCTTCGAGTCGTTGA (Saure03 (Saureus03), SEQ ID NO: 367).

다른 관점(42-ii)으로, 본 발명은 상기 서열번호 365 내지 367중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 스타필로코코스 아우레우스 균의 검출용 핵산 탐침을 제공한다.In another aspect (42-ii), the present invention provides a nucleic acid probe for detecting Staphylococcus aureus, including a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 365 to 367.

다른 관점(42-iii)으로, 본 발명은 상기 서열번호 365 내지 367중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 스타필로코코스 아우레우스 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (42-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Staphylococcus aureus bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 365 to 367.

다른 관점(42-iv)으로, 본 발명은 (a) 상기 서열번호 365 내지 367중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 스타필로코코스 아우레우스 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 스타필로코코스 아우레우스 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (42-iv), the present invention provides a composition comprising (a) a nucleic acid probe for detecting Staphylococcus aureus comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 365 to 367; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Staphylococcus aureus bacteria in a biological sample.

다른 관점(42-v)으로, 본 발명은 상기 서열번호 365 내지 367중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 스타필로코코스 아우레우스 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (42-v), the present invention provides a DNA in which a nucleic acid probe for detecting Staphylococcus aureus comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 365 to 367 is fixed to a solid support. Provide chips.

다른 관점(42-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 365 내지 367중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 스타필로코코스 아우레우스 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 스타필로코코스 아우레우스 균을 검출 및 동정하는 방법을 제공한다.In another aspect 42-vi, the present invention is directed to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) a composition comprising a nucleic acid probe for detecting Staphylococcus aureus, wherein the polynucleic acid of steps (a) and (b) comprises a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 365 to 367 Contacting with appropriate hybridization and washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Staphylococcus aureus bacteria in the biological sample.

다른 관점(43-i)으로, 본 발명은 나이세리아 메닌자이티디스 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect 43-i, the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from 23S rRNA of Neisseria meningitidis bacteria:

AGATGTGAGAGCATC (Nm002, 서열번호 377).AGATGTGAGAGCATC (Nm002, SEQ ID NO: 377).

다른 관점(43-ii)으로, 본 발명은 상기 서열번호 377의 염기 서열을 포함한 핵산 분자를 포함하는 나이세리아 메닌자이티디스 균의 검출용 핵산 탐침을 제공한다.In another aspect (43-ii), the present invention provides a nucleic acid probe for detection of Neisseria meningitidis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 377.

다른 관점(43-iii)으로, 본 발명은 상기 서열번호 377의 염기 서열을 포함한 핵산 분자를 포함하는 나이세리아 메닌자이티디스균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (43-iii), the present invention provides a composition comprising a nucleic acid probe for detection of Neisseria meningitidis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 377.

다른 관점(43-iv)으로, 본 발명은 (a) 상기 서열번호 377의 염기 서열을 포함한 핵산 분자를 포함하는 나이세리아 메닌자이티디스 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 나이세리아 메닌자이티디스 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (43-iv), the present invention (a) a composition comprising a nucleic acid probe for the detection of bacteria Neisseria meningitidis comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 377; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Neisseria meningitidis bacteria in biological samples.

다른 관점(43-v)으로, 본 발명은 상기 서열번호 377의 염기 서열을 포함한 핵산 분자를 포함하는 나이세리아 메닌자이티디스균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (43-v), the present invention provides a DNA chip in which a nucleic acid probe for detection of Neisseria meningitidis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 377 is fixed to a solid support.

다른 관점(43-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 377의 염기 서열을 포함한 핵산 분자를 포함하는 나이세리아 메닌자이티디스 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 나이세리아 메닌자이티디스 균을 검출 및 동정하는 방법을 제공한다.In another aspect (43-vi), the present invention provides a method for (a) isolating and / or concentrating polynucleic acid, optionally present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) appropriate hybridization and washing of the polynucleic acid of steps (a) and (b) with a composition comprising a nucleic acid probe for detection of Neisseria meningitidis bacteria comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 377 Contacting under conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Neisseria meningitidis bacteria in the biological sample.

다른 관점(44-i)으로, 본 발명은 레지오넬라 뉴모필라 균의 23S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect 44-i, the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from the Legionella pneumophila 23S rRNA:

TGGAGAGCATTTTAT (L.pneu011, 서열번호 383);TGGAGAGCATTTTAT (L.pneu011, SEQ ID NO: 383);

GTGATTTTGAGGTGA (L.pneu012, 서열번호 384); 또는GTGATTTTGAGGTGA (L.pneu012, SEQ ID NO: 384); or

AGATGGTAAAGAAGA (L.pneu013, 서열번호 385).AGATGGTAAAGAAGA (L.pneu013, SEQ ID NO: 385).

다른 관점(44-ii)으로, 본 발명은 상기 서열번호 383 내지 385중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 레지오넬라 뉴모필라 균의 검출용 핵산 탐침을 제공한다.In another aspect (44-ii), the present invention provides a nucleic acid probe for detecting Legionella pneumophila, comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 383 to 385.

다른 관점(44-iii)으로, 본 발명은 상기 서열번호 383 내지 385중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 레지오넬라 뉴모필라 균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (44-iii), the present invention provides a composition comprising a nucleic acid probe for detecting Legionella pneumophila comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 383 to 385.

다른 관점(44-iv)으로, 본 발명은 (a) 상기 서열번호 383 내지 385중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 레지오넬라 뉴모필라 균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 레지오넬라 뉴모필라 균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (44-iv), the present invention provides a composition comprising (a) a nucleic acid probe for detecting Legionella pneumophila comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 383 to 385; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for detecting and identifying Legionella pneumophila in a biological sample.

다른 관점(44-v)으로, 본 발명은 상기 서열번호 383 내지 385중의 어느 하나의 염기 서열을 포함한 핵산분자를 포함하는 레지오넬라 뉴모필라 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (44-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting Legionella pneumophila comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 383 to 385 is fixed to a solid support. do.

다른 관점(44-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 383 내지 385중의 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 레지오넬라 뉴모필라 균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 레지오넬라 뉴모필라 균을 검출 및 동정하는 방법을 제공한다.In another aspect 44-vi, the present invention is directed to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) appropriate hybridization of the polynucleic acid of steps (a) and (b) with a composition comprising a nucleic acid probe for detection of Legionella pneumophila comprising a nucleic acid molecule comprising any one of SEQ ID NOs: 383 to 385 And under washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Legionella pneumophila in the biological sample.

다른 관점(45-i)으로, 본 발명은 캔디다 알비칸스 진균의 18S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect 45-i, the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from the 18S rRNA of Candida albicans fungus:

TGGTAGCCATTTATG (C.alic 001, 서열번호 396);TGGTAGCCATTTATG (C.alic 001, SEQ ID NO: 396);

CTGGACCAGCCGAGC (C.alic 003, 서열번호 397);CTGGACCAGCCGAGC (C.alic 003, SEQ ID NO: 397);

TCAAGAACGAAAGTT (C.alic 006, 서열번호 398);TCAAGAACGAAAGTT (C.alic 006, SEQ ID NO: 398);

AAGGATTGACAGATT (C.alic 007, 서열번호 399); 또는AAGGATTGACAGATT (C.alic 007, SEQ ID NO: 399); or

CATTAATCAAGAACG (C.alic 008, 서열번호 400).CATTAATCAAGAACG (C.alic 008, SEQ ID NO: 400).

다른 관점(45-ii)으로, 본 발명은 상기 서열번호 396 내지 400중 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 캔디다 알비칸스 진균의 검출용 핵산 탐침을 제공한다.In another aspect (45-ii), the present invention provides a nucleic acid probe for the detection of Candida albicans fungus comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 396 to 400.

다른 관점(45-iii)으로, 본 발명은 상기 서열번호 396 내지 400중 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 캔디다 알비칸스 진균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (45-iii), the present invention provides a composition comprising a nucleic acid probe for the detection of Candida albicans fungi comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 396-400.

다른 관점(45-iv)으로, 본 발명은 (a) 상기 서열번호 396 내지 400중 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 캔디다 알비칸스 진균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 캔디다 알비칸스 진균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (45-iv), the present invention provides a composition comprising a nucleic acid probe for detecting Candida albicans fungi comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 396 to 400; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, providing a kit for detecting and identifying Candida albicans fungi in a biological sample.

다른 관점(45-v)으로, 본 발명은 상기 서열번호 396 내지 400중 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 캔디다 알비칸스 진균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (45-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting Candida albicans fungi comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 396 to 400 is immobilized on a solid support. do.

다른 관점(45-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 396 내지 400중 어느 하나의 염기 서열을 포함한 핵산 분자를 포함하는 캔디다 알비칸스 진균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 캔디다 알비칸스 진균을 검출 및 동정하는 방법을 제공한다.In another aspect (45-vi), the present invention provides a method for (a) isolating and / or concentrating polynucleic acid, optionally present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) appropriate hybridization of the polynucleic acid of steps (a) and (b) with a composition comprising a nucleic acid probe for the detection of Candida albicans fungi comprising a nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 396-400 And under washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Candida albicans fungi in the biological sample.

다른 관점(46-i)으로, 본 발명은 캔디다 글라브라타 진균의 18S rRNA로부터 유도된 하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자를 제공한다:In another aspect 46-i, the present invention provides an isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following base sequence derived from the 18S rRNA of Candida glabrata fungus:

CTGGAATGCACCCGG (C.glab 001, 서열번호 404); 또는CTGGAATGCACCCGG (C.glab 001, SEQ ID NO: 404); or

TGGCTTGGCGGCGAA (C.glab 003, 서열번호 405).TGGCTTGGCGGCGAA (C.glab 003, SEQ ID NO: 405).

다른 관점(46-ii)으로, 본 발명은 상기 서열번호 404 또는 405의 염기 서열을 포함한 핵산 분자를 포함하는 캔디다 글라브라타 진균의 검출용 핵산 탐침을 제공한다.In another aspect 46-ii, the present invention provides a nucleic acid probe for the detection of Candida glabrata fungi comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 404 or 405.

다른 관점(46-iii)으로, 본 발명은 상기 서열번호 404 또는 405의 염기 서열을 포함한 핵산 분자를 포함하는 캔디다 글라브라타 진균의 검출용 핵산 탐침을 포함한 조성물을 제공한다.In another aspect (46-iii), the present invention provides a composition comprising a nucleic acid probe for the detection of Candida glabrata fungi comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 404 or 405.

다른 관점(46-iv)으로, 본 발명은 (a) 상기 서열번호 404 또는 405의 염기 서열을 포함한 핵산 분자를 포함하는 캔디다 글라브라타 진균의 검출용 핵산 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 캔디다 글라브라타 진균을 검출 및 동정하기 위한 키트를 제공한다.In another aspect (46-iv), the present invention provides a composition comprising a nucleic acid probe for detecting Candida glabrata fungi comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 404 or 405; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a previously formed hybrid, providing a kit for detecting and identifying Candida glabrata fungi in a biological sample.

다른 관점(46-v)으로, 본 발명은 상기 서열번호 404 또는 405의 염기 서열을 포함한 핵산 분자를 포함하는 캔디다 글라브라타 진균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In another aspect (46-v), the present invention provides a DNA chip in which a nucleic acid probe for detecting Candida glabrata fungi comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 404 or 405 is fixed to a solid support.

다른 관점(46-vi)으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 서열번호 404 또는 405의 염기 서열을 포함한 핵산 분자를 포함하는 캔디다 알비칸스 진균의 검출용 핵산 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 캔디다 글라브라타 진균을 검출 및 동정하는 방법을 제공한다.In another aspect 46-vi, the present invention is directed to (a) optionally separating and / or concentrating polynucleic acid present in a biological sample, and (b) optionally amplifying the polynucleic acid with the appropriate forward and reverse primer pairs; (c) appropriate hybridization and washing conditions with a composition comprising a nucleic acid probe for the detection of Candida albicans fungus comprising the nucleic acid molecule comprising the nucleic acid sequence of SEQ ID NO: 404 or 405 in the polynucleic acid of steps (a) and (b) Contacting under; (d) detecting the hybrid formed in step (c); (e) identifying a strain present in the sample from the different hybridization signals obtained in step (d), thereby providing a method for detecting and identifying Candida glabrata fungi in the biological sample.

추가의 관점으로, 본 발명은 상기 탐침중에서 선택된 2종 이상의 탐침을 포함한 조성물을 제공한다.In a further aspect, the present invention provides a composition comprising at least two probes selected from said probes.

다른 추가의 관점으로, 본 발명은 상기 탐침중에서 선택된 2종 이상의 탐침을 포함한 조성물; (b) 임의로 생물학적 시료중의 폴리핵산을 증폭하는데 사용하는 전방향과 역방향 프라이머 쌍; (c) 상기 조성물에 함유된 탐침과 생물학적 시료중에 존재하는 폴리핵산 또는 이의 증폭된 산물사이에 혼성화 반응을 유도하는 완충액 또는 이러한 완충액을 생성하는데 필요한 성분; (d) 적당한 세척 조건하에서 형성된 하이브리드를 세척하기 위한 용액 또는 이러한 용액을 생성하는데 필요한 성분; 및 (e) 임의로 앞서 형성된 하이브리드를 검출하는 수단을 포함함을 특징으로 하여, 생물학적 시료에서 상기 균중 2종 이상을 동시에 검출 및 동정하기 위한 키트를 제공한다.In another further aspect, the present invention provides a composition comprising two or more probes selected from the above probes; (b) forward and reverse primer pairs optionally used to amplify polynucleic acid in a biological sample; (c) a buffer or component necessary to produce such a buffer to induce a hybridization reaction between the probe contained in the composition and the polynucleic acid or amplified product thereof present in the biological sample; (d) a solution for washing the hybrid formed under suitable washing conditions or the components necessary to produce such a solution; And (e) optionally means for detecting a hybrid previously formed, thereby providing a kit for simultaneously detecting and identifying two or more of said bacteria in a biological sample.

다른 추가의 관점으로, 본 발명은 상기 탐침중에서 선택된 2종 이상의 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다.In a still further aspect, the present invention provides a DNA chip in which at least two probes selected from the probes are fixed to a solid support.

다른 추가의 관점으로, 본 발명은 (a) 임의로 생물학적 시료로부터 중에 존재하는 폴리핵산을 분리 및/또는 농축하고, (b) 임의로 폴리핵산을 적절한 전방향과 역방향 프라이머 쌍으로 증폭하고; (c) 상기 단계 (a) 및 (b)의 폴리핵산을 상기 탐침중에서 선택된 2종 이상의 탐침을 포함한 조성물과 적절한 혼성화 및 세척 조건하에서 접촉시키고; (d) 상기 (c) 단계에서 형성된 하이브리드를 검출하고; (e) 상기 (d) 단계에서 얻은 상이한 혼성화 시그날로부터 시료에 존재하는 균주를 동정함을 특징으로 하여, 상기 생물학적 시료중에서 상기 균중 2종 이상을 동시에 검출 및 동정하는 방법을 제공한다.In another further aspect, the present invention provides a method for the preparation of (a) isolating and / or concentrating polynucleic acid, optionally present in a biological sample, and (b) optionally amplifying the polynucleic acid with appropriate forward and reverse primer pairs; (c) contacting the polynucleic acid of steps (a) and (b) with a composition comprising two or more probes selected from the probes under appropriate hybridization and washing conditions; (d) detecting the hybrid formed in step (c); (e) identifying the strains present in the sample from the different hybridization signals obtained in step (d), thereby providing a method of simultaneously detecting and identifying two or more of said bacteria in said biological sample.

용어 정의Term Definition

다음의 정의는 아래 기술된 바와 같이 본 발명의 다른 양태에서 사용된 용어 및 표현을 설명해 준다.The following definitions describe terms and expressions used in other aspects of the invention as described below.

"분리된" 핵산 분자는 핵산의 천연원에 존재하는 다른 핵산 분자로부터 분리된 것이다. 예를 들면, 게놈 DNA와 관련하여, 용어 "분리된"은 게놈 DNA가 천연적으로 결합하고 있는 염색체로부터 분리된 핵산 분자를 포함한다.A "isolated" nucleic acid molecule is one that is separated from other nucleic acid molecules present in the natural source of the nucleic acid. For example, with respect to genomic DNA, the term “isolated” includes nucleic acid molecules isolated from chromosomes to which genomic DNA is naturally bound.

"탐침" 또는 "핵산 탐침"은 검출하고자 하는 표적 염기 서열과 충분히 상보적이어서 혼성화하는 염기 서열을 갖는 일본쇄-특이적 올리고뉴클레오타이드를 가리킨다."Probe" or "nucleic acid probe" refers to a single-chain oligonucleotide having a base sequence that is sufficiently complementary to the target base sequence to be detected and hybridizes.

"조성물"은 세균 또는 진균의 rRNA에 상보적인 탐침이 순수한 상태이거나 다른 탐침과 배합되어 있을 수 있음을 의미한다. 또한, 탐침은 염 또는 완충제와 배합되어 건조된 상태, 침전물로서 알코올 용액 또는 수용액의 상태로 있을 수 있다."Composition" means that a probe complementary to a bacterial or fungal rRNA may be pure or combined with other probes. In addition, the probe may be combined with a salt or a buffer in a dried state, as a precipitate, in the form of an alcohol solution or an aqueous solution.

"표적"은 본 발명에 따른 탐침중 어느 것과도 상보적인 서열을 갖는 생물학적 시료 유래의 핵산 분자를 의미한다. 표적 핵산은 일본쇄 또는 이본쇄의 DNA (임의로 증폭 후 수득함)이거나 RNA일 수 있으며 적어도 한 개의 탐침 올리고뉴클레오타이드와 적어도 부분적인 상보성을 갖는 서열을 함유한다."Target" means a nucleic acid molecule from a biological sample having a sequence complementary to any of the probes according to the invention. The target nucleic acid may be single stranded or double stranded DNA (optionally obtained after amplification) or RNA and contains a sequence having at least partial complementarity with at least one probe oligonucleotide.

"생물학적 시료"는 목적하는 표적 서열을 검출하고자 하는 임상 시료 (예, 농즙, 타액, 혈액, 뇨 등), 환경 시료, 세균 콜로니, 오염된 또는 순수한 배양물, 정제된 핵산 등의 검체 (specimen)를 가리킨다.A "biological sample" is a sample of a clinical sample (eg, juice, saliva, blood, urine, etc.), environmental sample, bacterial colony, contaminated or pure culture, purified nucleic acid, etc., for which a target sequence of interest is to be detected. Point to.

"폴리핵산"은 적어도 10, 20, 30, 40 또는 50개의 뉴클레오타이드가 연속하고 있는 일본쇄 또는 이본쇄 cDNA 또는 게놈 DNA 또는 RNA에 상응한다. 뉴클레오타이드의 길이가 100개 이하인 폴리핵산은 흔히 올리고뉴클레오타이드라고 한다. 일본쇄 폴리핵산 서열은 본 발명에서 항상 5' 말단으로부터 3' 말단으로 표시된다."Polynucleic acid" corresponds to a single-stranded or double-stranded cDNA or genomic DNA or RNA in which at least 10, 20, 30, 40 or 50 nucleotides are contiguous. Polynucleic acids having a length of 100 or fewer nucleotides are often referred to as oligonucleotides. Single-chain polynucleic acid sequences are always represented from the 5 'end to the 3' end in the present invention.

"올리고뉴클레오타이드"는 일반적으로 약 10개 내지 약 100개의 뉴클레오타이드로 구성된 뉴클레오타이드 고분자를 의미한다. 그러나, 뉴클레오타이드의 길이는 100개 이상이거나 10개 이하일 수 있다."Oligonucleotide" generally refers to a nucleotide polymer consisting of about 10 to about 100 nucleotides. However, the length of the nucleotides may be 100 or more or 10 or less.

"뉴클레오타이드"는 포스페이트 그룹, 5-탄당 및 질소 염기로 구성된 핵산의 기본 단위이다. RNA에서 5-탄당은 리보스이다. DNA에서 5-탄당은 2-데옥시리보스이다. 5-뉴클레오타이드의 경우, 당은 5-탄당-2에서 하이드록실그룹(-OH)을 함유한다. 이 용어는 또한 리보스의 2 위치에 메톡시 그룹과 같이 상기 기본 단위의 유사체를 포함한다.A “nucleotide” is the basic unit of nucleic acid consisting of phosphate groups, 5-carbosaccharides and nitrogen bases. 5-Tanose in RNA is ribose. The 5-saccharide in DNA is 2-deoxyribose. In the case of 5-nucleotides, the sugars contain hydroxyl groups (-OH) in 5-saccharide-2. The term also includes analogs of these basic units, such as methoxy groups at the 2 position of ribose.

"상동성"은 동일성과 동의어로서, 예를 들면 90% 상동성이라고 하는 폴리핵산은 서열의 배열시 동일한 위치에서 90%의 동일한 염기쌍을 나타내는 것을 의미한다."Homologous" is synonymous with identity, meaning that a polynucleic acid, for example 90% homology, exhibits 90% identical base pairs at the same position in the arrangement of the sequences.

"혼성화"는 표적 핵산(검출하고자 하는 서열)에 상보 서열의 교잡(annealing)을 의미한다. 상보 서열을 포함한 두 핵산 고분자가 서로를 발견하고 염기 쌍 상호작용을 통해 교잡하는 능력은 잘 알려진 현상이다."Hybridization" means the annealing of complementary sequences to a target nucleic acid (the sequence to be detected). The ability of two nucleic acid polymers, including complementary sequences, to find each other and hybridize through base pair interactions is a well known phenomenon.

"프라이머"는 복제하고자 하는 핵산 본쇄에 상보적인 연장 산물의 합성을 위한 개시점으로 작용할 수 있는 일본쇄 DNA 올리고뉴클레오타이드 서열을 가리킨다. 프라이머의 길이 및 서열은 이들이 연장 산물의 합성을 개시할 수 있을 정도이면 되는데 바람직하게는 약 5 내지 50개의 뉴클레오타이드로 구성된다. 특정한 길이 및 서열은 목적하는 DNA 또는 RNA 표적의 복잡성뿐만 아니라 온도 및 이온강도와 같은 프라이머 사용의 조건에 의해 결정될 것이다."Primer" refers to a single stranded DNA oligonucleotide sequence that can serve as a starting point for the synthesis of extension products complementary to the nucleic acid backbone to be replicated. The length and sequence of the primers may be such that they can initiate the synthesis of extension products, preferably consisting of about 5 to 50 nucleotides. The specific length and sequence will be determined by the complexity of the desired DNA or RNA target as well as the conditions of primer use such as temperature and ionic strength.

"긴축"은 혼성화 및 후속 과정동안의 온도 및 용매 조성을 기술하기 위해 사용된다. 고도의 긴축 조건하에서 단지 고도로 상보적인 핵산 하이브리드가 형성될 것이다. 반대로, 충분한 정도의 상보성이 결여되어서는 하이브리드는 형성되지 않는다. 따라서, 검정 조건의 긴축은 하이브리드를 형성하는 두 핵산 본쇄사이에 필요한 상보성의 양을 결정한다. 긴축은 표적 핵산 및 비표적 핵산과 형성과 형성된 하이브리드간에 안정성의 차이를 최대화하는데서 선택된다."Tension" is used to describe the temperature and solvent composition during hybridization and subsequent processing. Under highly tightening conditions only highly complementary nucleic acid hybrids will be formed. In contrast, hybrids do not form when they lack a sufficient degree of complementarity. Thus, the tightening of the assay conditions determines the amount of complementarity required between the two nucleic acid backbones that form the hybrid. Contraction is chosen to maximize the difference in stability between the target and non-target nucleic acids and the hybrids formed and formed.

"상보성"은 개개 본쇄의 왓슨-크릭 염기 쌍사이에 수소 결합을 통해 하이브리드 또는 이본쇄 DNA:DNA, RNA:RNA 또는 DNA:RNA를 형성할 수 있는 DNA 또는 RNA의 일본쇄의 염기 서열에 의해 제공되는 성질을 가리킨다. 아데닌(A)은 통상 티민(T) 또는 우라실(U)과 상보적이고 구아닌(G)은 통상 시토신(C)과 상보적이다.“Complementarity” is provided by the base sequence of the Japanese chain of DNA or RNA capable of forming hybrid or double-stranded DNA: DNA, RNA: RNA or DNA: RNA via hydrogen bonding between individual stranded Watson-Crick base pairs. It indicates the nature of being. Adenine (A) is usually complementary to thymine (T) or uracil (U) and guanine (G) is usually complementary to cytosine (C).

"부정합"은 하이브리드에서 정상적인 왓슨-크릭 수소 결합을 형성하지 않는 두 뉴클레오타이드의 모든 쌍을 가리킨다."Matched" refers to all pairs of two nucleotides that do not form normal Watson-Crick hydrogen bonds in a hybrid.

"표지"는 검출가능한 (바람직하게는 정량가능한) 시그날을 제공하고 핵산에 결합될 수 있는 모든 원자 또는 분자를 가리킨다. 표지는 형광, 방사성, 색도, X-선 회절 또는 흡수, 마그네티즘 등에 의해 검출가능한 시그날을 제공할 수 있다.A "label" refers to any atom or molecule capable of providing a detectable (preferably quantifiable) signal and capable of binding to a nucleic acid. The label can provide a signal detectable by fluorescence, radioactivity, chromaticity, X-ray diffraction or absorption, magnetism, and the like.

"하이브리드"는 상보적인 염기사이의 왓슨-크릭 염기 쌍 또는 비표준 염기 쌍에 의해 두개의 일본쇄 핵산 서열사이에 형성된 복합체이다.A "hybrid" is a complex formed between two single-stranded nucleic acid sequences by Watson-Crick base pairs or non-standard base pairs between complementary bases.

"탐침 특이성"은 표적과 비표적 서열을 구분하는 능력을 기술하기 위한 탐침의 특징을 가리킨다. 이와 관련하여, 탐침이 특이적이다 라고 하는 것은 뉴클레오타이드 서열이 정해진 표적 서열과 혼성화하고 비표적 서열과는 실질적으로 하이브리드하지 않거나 비표적 서열과의 혼성화가 최소로 이루어진다는 것을 의미한다. 탐침 특이성은 서열 및 검정 조건에 의해 좌우된다."Probe specificity" refers to the characteristics of a probe to describe its ability to distinguish between target and nontarget sequences. In this regard, the fact that the probe is specific means that the nucleotide sequence hybridizes with a given target sequence and is substantially hybridized with the nontarget sequence or with minimal hybridization with the nontarget sequence. Probe specificity depends on sequence and assay conditions.

"Tm"은 탐침의 50%가 혼성화 형태로부터 비혼성화 형태로 전환되는 시점의 온도를 가리킨다."Tm" refers to the temperature at which 50% of the probe is converted from the hybridized form to the unhybridized form.

"표준균주"는 시판중에 있거나 용이하게 입수가능한 균주를 가리킨다."Standard strain" refers to a strain that is commercially available or readily available.

탐침의 동정Sympathy

균의 검출용 핵산 탐침을 제작하기 위해서는 각 균에만 특이성을 가져야 하는데 이를 위하여 먼저 각 균에 대한 특이 탐침 후보군을 선정한다. 특이 탐침 후보군은 가능한 모든 균들의 염기서열들을 다중 정렬(multiple alignment)을 통해서 일렬로 정렬 비교하여 각 균에만 존재하는 특이적인 염기서열을 별도로 구분하여 그 내부에 존재하는 탐침 후보군을 제작하여 선별한다. 탐침 후보군은 세균의 경우 각 균내의 23S rRNA 유전자 및/또는 ITS 부위 내에서 결정하고, 진균의 경우는 18S rRNA 유전자 부위 내에서 결정한다. 탐침 후보군의 특이성 여부는 먼저 BLAST 검색을 통해 균별간 유사성을 비교하여 확인하고 실제로 혼성화 반응에 균주들을 적용하여 각 균에서만 반응하는 탐침을 후보군 내에서 동정용으로 선별한다. 추가로, 상기와 같이 선별된 동정용 핵산 탐침은 여러 생물학적 시료를 대상으로 하는 임상 시험에 적응하여 민감성을 확인한다.In order to produce a nucleic acid probe for the detection of bacteria, it is necessary to have specificity only for each bacteria. For this purpose, a specific probe candidate group for each bacteria is selected. The specific probe candidate group sorts and compares the sequences of all possible bacteria in a line through multiple alignments to separately classify specific sequences present only in each of the bacteria to prepare and select a probe candidate group present therein. Probe candidates are determined within the 23S rRNA gene and / or ITS site in each bacterium for bacteria and within the 18S rRNA gene site for fungi. The specificity of the probe candidate group is first confirmed by comparing the similarities between the bacterial strains through BLAST search, and the strains are applied to the hybridization reaction, and the probes that react only with each bacteria are selected for identification in the candidate group. In addition, the nucleic acid probes selected as described above are adapted to clinical trials involving multiple biological samples to confirm their sensitivity.

본 발명에 따라 선별된 핵산 탐침은 적어도 15량체의 올리고뉴클레오타이드이며, 바람직하게는 검출하고자 하는 표적의 완전한 상보서열과 70%, 80%, 90% 또는 95% 이상의 상동성을 갖는다. 본 발명에 따른 각 균의 검출 및 동정용 핵산 탐침의 올리고뉴클레오타이드의 길이는 50개 또는 그 이상도 가능하다. 본 발명에 사용되는 뉴클레오타이드는 리보뉴클레오타이드, 데옥시리보뉴클레오타이드 및 이노신 또는 변형 그룹을 함유한 뉴클레오타이드와 같은 변형된 뉴클레오타이드도 가능하나, 이들은 혼성화 특징에 반드시 영향을 미치지 않아야 한다.The nucleic acid probes selected according to the invention are at least 15 oligonucleotides of oligonucleotide and preferably have at least 70%, 80%, 90% or 95% homology with the full complement sequence of the target to be detected. The length of the oligonucleotide of the nucleic acid probe for detecting and identifying each bacterium according to the present invention may be 50 or more. The nucleotides used in the present invention may also be modified nucleotides such as ribonucleotides, deoxyribonucleotides and nucleotides containing inosine or modified groups, but they must not affect hybridization characteristics.

탐침 이용Probe

본 발명의 핵산 탐침은 진단 목적상 생물학적 시료중에서 표적 핵산의 유무를 시험하는데 있어서 공지된 모든 혼성화 기술, 예를 들면 도트-블롯이라고 하는 필터상에의 포인트 침착 기술(MANIATIS et al., Molecular Cloning, Cold Spring Harbor, 1982), 써던 블롯이라고 하는 DNA 전달 기술 (SOUTHERN, E.M., J. Mol. Biol., 98, 503 (1975)), 노썬 블롯이라고 하는 RNA 전달 기술에 따라 사용할 수 있다.Nucleic acid probes of the present invention can be used for all known hybridization techniques for testing the presence or absence of a target nucleic acid in a biological sample for diagnostic purposes, such as point deposition on a filter called dot-blot (MANIATIS et al., Molecular Cloning, Cold Spring Harbor, 1982), a DNA transfer technique called Southern blot (SOUTHERN, EM, J. Mol. Biol., 98, 503 (1975)), and an RNA transfer technique called Nothon blot.

또한, 본 발명의 핵산 탐침은 핵산 탐침-기본 검정의 특이성을 높여주는 샌드위치 혼성화 시스템에 사용할 수 있다. 핵산 탐침-기본 검정에서 샌드위치 혼성화의 원리 및 사용은 공지되어 있다(DUNN and HASSEL, Cell, 12: 23-36, 1977; 및 RNAKI et al., Gene, 21: 77-85, 1983). 샌드위치 혼성화 기술은 포획 탐침 및/또는 검출 탐침이 사용되며 이들 탐침은 표적 핵산의 상이한 두 영역과 혼성화할 수 있고 그들 중 적어도 하나 (일반적으로 검출 탐침)가 목적하는 균에 대해 특이적인 표적의 영역과 혼성화할 수 있다. 포획 탐침과 검출 탐침은 적어도 부분적으로 상이한 뉴클레오타이드 서열을 가져야 한다. 직접적인 혼성화 검정이 유리한 역학을 제시하지만, 샌드위치 혼성화는 시그날-대-노이즈 비율이 높다는 점에서 유리하다. 또한, 샌드위치 혼성화는 핵산 탐침-기본 검정의 특이성을 높일 수 있다. 샌드위치 혼성화 과정의 주요 단계를 구성하는 배양 및 후속 세척 단계는 각각 항온, 약 20 내지 65℃에서 실시한다. 핵산 하이브리드는 혼성화된 염기의 수에 의해 좌우되고 (온도는 하이브리드의 크기에 비례하여 증가한다) 또한 혼성화된 염기의 성질 및 각각의 혼성화된 염기의 경우 인접한 염기의 성질에 의해 좌우되는 해리 온도를 갖는 것으로 알려져 있다. 샌드위치 혼성화 기술에서 사용되는 혼성화 온도는 주어진 탐침과 상보적인 서열의 표적사이에 형성된 하이브리드의 반-해리 온도 이하에서 선택되어야 한다. 이러한 반-해리 온도는 간단한 통상의 실험에 의해 결정할 수 있다.In addition, the nucleic acid probes of the present invention can be used in sandwich hybridization systems that enhance the specificity of nucleic acid probe-based assays. The principle and use of sandwich hybridization in nucleic acid probe-based assays is known (DUNN and HASSEL, Cell, 12: 23-36, 1977; and RNAKI et al., Gene, 21: 77-85, 1983). The sandwich hybridization technique employs a capture probe and / or a detection probe, which can hybridize with two different regions of the target nucleic acid and at least one of them (generally the detection probe) has a region of the target specific for the desired bacterium. May hybridize. Capture probes and detection probes should have at least partially different nucleotide sequences. While direct hybridization assays offer advantageous kinetics, sandwich hybridization is advantageous in that it has a high signal-to-noise ratio. In addition, sandwich hybridization can increase the specificity of nucleic acid probe-based assays. Incubation and subsequent washing steps, which constitute the main step of the sandwich hybridization process, are carried out at constant temperature, about 20-65 ° C., respectively. Nucleic acid hybrids are dependent on the number of hybridized bases (the temperature increases in proportion to the size of the hybrid) and also have a dissociation temperature that is dependent on the nature of the hybridized base and the nature of the adjacent base for each hybridized base. It is known. The hybridization temperature used in the sandwich hybridization technique should be selected below the half-dissociation temperature of the hybrid formed between the given probe and the target of the complementary sequence. This semi-dissociation temperature can be determined by simple routine experimentation.

또한, 본 발명의 핵산 탐침은 경쟁 혼성화 프로토콜에 사용할 수 있다. 경쟁 혼성화에서, 표적 분자는 특정 탐침과 이의 상보체사이에서의 하이브리드 형성과 경쟁한다. 표적이 더 많이 존재할수록 탐침과 이의 상보체사이에 형성된 하이브리드의 양은 줄어든다. 특정 표적이 존재함을 지시하는 양성 시그날은 표적이 첨가되지 않은 시스템과 비교하여 혼성화 반응이 감소하는 것으로 나타난다. 특정 양태로서, 편리하게는 표지되는 특이적 올리고뉴클레오타이드 탐침을 표적 분자와 혼성화한다. 이어서, 혼합물을 특이적 탐침과 상보적인 올리고뉴클레오타이드가 고정된 미세역가 디쉬 웰에 넣고 계속 혼성화 반응이 일어나도록 한다. 세척 후 상보적인 올리고뉴클레오타이드와 탐침사이의 하이브리드를 바람직하게는 사용된 표지에 따라 정량한다.In addition, the nucleic acid probes of the invention can be used in a competitive hybridization protocol. In competitive hybridization, the target molecule competes with hybrid formation between a particular probe and its complement. The more targets are present, the less hybrid is formed between the probe and its complement. Positive signals indicating the presence of a specific target appear to decrease the hybridization response compared to a system without a target added. In certain embodiments, conveniently labeled specific oligonucleotide probes are hybridized with a target molecule. Subsequently, the mixture is placed in a microtiter dish well immobilized with oligonucleotides complementary to the specific probe, allowing the hybridization to continue. After washing, the hybrid between the complementary oligonucleotide and the probe is preferably quantified according to the label used.

또한, 본 발명의 핵산 탐침은 역혼성화 (reversed hybridization) (Proc. Natl. Acad. Sci. USA, 86:6230-6234, 1989)에 사용할 수 있다. 이 경우는 우선 표적 서열을 5 바이오티닐화 프라이머를 사용하여 PCR을 수행함으로써 효소적으로 증폭할 수 있다. 두 번째 단계로 증폭된 산물은 고체 지지체상에 고정된 특정 올리고뉴클레오타이드와의 혼성시켜 검출한다. 역혼성화는 증폭하지 않고서 실시할 수 있다. 이러한 경우는 생물학적 시료에 존재하는 핵산은 혼성화이전에 화학적으로나 특정 염료을 첨가하여 특이적으로 또는 비특이적으로 표지 또는 변형시켜야 한다.In addition, the nucleic acid probes of the present invention can be used for reversed hybridization (Proc. Natl. Acad. Sci. USA, 86: 6230-6234, 1989). In this case, first, the target sequence can be enzymatically amplified by performing PCR using 5 biotinylated primers. The product amplified in the second step is detected by hybridization with specific oligonucleotides immobilized on a solid support. Reverse hybridization can be carried out without amplification. In such cases, the nucleic acid present in the biological sample must be labeled or modified specifically or nonspecifically by chemical or specific dye addition prior to hybridization.

본 발명의 핵산 탐침은 상기 본 발명에서 목적하는 병원균의 존재 여부를 신속히 결정하는데 사용할 수 있는 키트에 포함될 수 있다. 키트는 병원균의 존재를 검정하는데 필요한 모든 구성요소를 포함한다. 통상의 개념으로, 키트는 표지된 탐침의 안정한 제제, 표적과 탐침 핵산의 혼성화를 유도하기 위한 무수 또는 액체 형태의 혼성화액, 원치않거나 혼성화되지 않은 핵산을 세척하여 제거하기 위한 용액, 표지된 하이브리드를 검출하기 위한 기질 및 임의로 표지를 검출하기 위한 기기를 포함한다.The nucleic acid probe of the present invention can be included in a kit that can be used to quickly determine the presence or absence of a pathogen of interest in the present invention. The kit includes all the components necessary to assay for the presence of the pathogen. In a conventional concept, a kit may contain a stable preparation of a labeled probe, a hybridization solution in anhydrous or liquid form to induce hybridization of a target and probe nucleic acid, a solution for washing and removing unwanted or unhybridized nucleic acid, and a labeled hybrid. Substrates for detection and optionally instruments for detecting labels.

본 발명의 한 가지 특정 양태는 샌드위치 검정의 개념을 이용하는 키트를 포함한다. 이 키트는 환자의 특정 부위에서 검체를 수집하기 위한 제1 요소, 예를들면 검체의 스크랩핑 도구 또는 페이터 포인트, 수용 바이알, 분산 및 용해 완충액이 포함된다. 제2 요소는 표적과 탐침 핵산사이의 혼성화를 위한 무수 또는 액체 상태의 매질 및 혼성화되지 않고 원하지 않는 형태를 세척하여 제거하기 위한 매질을 포함한다. 제3 요소는 표적 핵산의 일부에 상보적인 비표지된 핵산 탐침이 고정되어 있거나 결합되어 있는 고체 지지체를 포함한다. 다수의 표적을 분석하는 경우는 개개 자신의 rRNA에 대해 특이적인 한종 이상의 포획 탐침이 딥스틱의 다른 개별 영역에 적용된다. 제4 요소는 제3 요소의 고정되고 비표지된 핵산 탐침이 혼성화되는 동일한 rRNA 본쇄의 제2의 다른 영역에 상보적인 표지된 탐침을 함유한다. 여기서, 핵산 탐침은 동결건조된 핵산과 같은 무수 형태 또는 알코올 침전된 핵산과 같은 침전형태 또는 완충액으로서 탐침의 배합물을 포함한다. 표지된 알려진 어떠한 표지도 가능할 수 있다. 예를 들면, 탐침은 통상적인 수단에 의해 바이오티닐화할 수 있으며 바이오티닐화된 탐침의 존재는 고추냉이 퍼옥시다제와 같은 효소에 결합된 아비딘을 첨가한 다음, 퍼옥시다제와 반응했을 때 시각적으로 모니터링하거나 색도계 또는 분광계를 이용한 장비로 모니터링할 수 있는 기질과 접촉시켜 검출할 수 있다. 이러한 표지 방법 및 기타 효소 형태의 표지는 방사능 표지방법과 비교하여 경제적이고, 고도로 민감하며, 비교적 안전하다는 이점을 갖는다. 검정 키트의 완성품에는 표지된 탐침의 검출을 위한 여러 시약 및 기타 키트에 포함되는 것들, 예를 들면 지침서, 양성 및 음성 대조군 및 여러 성분을 혼합 반응시키기 위한 용기 등이 포함된다.One particular embodiment of the present invention includes a kit that utilizes the concept of a sandwich assay. The kit includes a first element for collecting a sample at a particular site of a patient, such as a sample scraping tool or patter point, a receiving vial, dispersion and lysis buffer. The second element includes an anhydrous or liquid medium for hybridization between the target and the probe nucleic acid and a medium for washing and removing unhybridized and unwanted forms. The third element comprises a solid support on which an unlabeled nucleic acid probe complementary to a portion of the target nucleic acid is immobilized or bound. In the case of analyzing multiple targets, one or more capture probes specific for their respective rRNAs are applied to different individual regions of the dipstick. The fourth element contains a labeled probe that is complementary to a second, different region of the same rRNA backbone where the fixed and unlabeled nucleic acid probe of the third element hybridizes. Here, the nucleic acid probe includes a combination of the probe either in anhydrous form such as lyophilized nucleic acid or in precipitate form such as alcohol precipitated nucleic acid or as a buffer. Any known label labeled may be possible. For example, the probe can be biotinylated by conventional means and the presence of the biotinylated probe is visually noticed when the avidin bound to an enzyme such as horseradish peroxidase is added and then reacted with the peroxidase. Detection can be made by contacting with a substrate that can be monitored or monitored using a colorimeter or spectrometer. Such labeling methods and labels of other enzyme forms have the advantage of being economical, highly sensitive and relatively safe compared to radiolabeling methods. The finished product of the assay kit includes various reagents for the detection of labeled probes and those included in other kits, such as instructions, positive and negative controls, and containers for mixing and reacting the various components.

DNA 칩DNA chip

또한, 본 발명의 핵산 탐침은 유전자 칩으로 사용된다. 본 발명의 바람직한 양태는 본 발명의 핵산 탐침이 고체 지지체에 고정된 DNA 칩을 제공한다. DNA 칩은 작은 기판상에 여러 염기 서열의 단편이 고밀도로 집적된 것으로 기판에 고정된 DNA와 이에 상보적인 미지의 DNA 시료사이의 혼성화에 의해 미지 시료의 DNA를 검출하는데 사용한다. 탐침을 고정시킬 기판은 유리, 실리콘 등과 같은 무기질이나 또는 아크릴계, 폴리에틸렌 테레프탈레이트 (polyethylene terephtalate, PET), 폴리스틸렌 (polystylene), 폴리카보네이트 (polycarbonate), 폴리프로필렌 (polypropylene) 등과 같은 고분자 물질로 이루어지고, 기판의 표면은 편평하거나 다수개의 구멍(hole)이 있는 것을 사용할 수 있다. 탐침의 고정은 5' 또는 3' 중 어느 한 위치가 기판에 공유결합으로 고정된다. 고정화 방법은 통상의 기술로서 예를 들면 정전기적 힘을 이용하거나, 합성된 올리고상에 아민기를 붙여 알데하이드 코팅된 슬라이드에 붙여 이 둘간의 결합을 유도하거나, 아민기 코팅된 슬라이드상에 혹은 L-라이신이 코팅된 슬라이드상에 혹은 니트로셀룰로즈 막이 코팅된 슬라이드 상에 집적함으로써 이루어질 수 있다. 본 발명의 한 양태로서, 탐침을 합성할 때 3' 위치에 아민기 (amino residue)를 가진 염기를 삽입하여 알데하이드 잔기로 코팅된 유리판에 공유결합 되도록 한다.In addition, the nucleic acid probe of the present invention is used as a gene chip. A preferred embodiment of the present invention provides a DNA chip in which the nucleic acid probe of the present invention is immobilized on a solid support. A DNA chip is a high density accumulation of fragments of several nucleotide sequences on a small substrate and is used to detect DNA of an unknown sample by hybridization between DNA immobilized on a substrate and an unknown DNA sample complementary thereto. The substrate on which the probe is to be fixed is made of an inorganic material such as glass or silicon, or a polymer material such as acrylic, polyethylene terephtalate (PET), polystylene, polycarbonate, polypropylene, etc. The surface of the substrate may be flat or have a plurality of holes. The probe is fixed either covalently to the substrate at either 5 'or 3'. The immobilization method is a conventional technique, for example using an electrostatic force or by attaching an amine group on the synthesized oligo to an aldehyde coated slide to induce bonding between the two, or on an amine group coated slide or L-lysine It can be done by integrating on this coated slide or on a slide with a nitrocellulose membrane coated. In one embodiment of the present invention, when the probe is synthesized, a base having an amino residue in the 3 'position is inserted to covalently bond to a glass plate coated with an aldehyde residue.

기판 표면에 각기 다른 탐침들을 고정, 배열하는 것은 핀 마이크로어레이, 잉크젯, 포토리소그래피, 전기적 어레이 등의 방법을 사용한다. 본 발명의 한 양태로는 탐침을 완충용액에 용해시킨 상태로 공지방법에 따라 제작된 마이크로어레이어(microarrayer)를 이용하여(Yoon et al, J. Microbiol. Biotechnol., 10(1), 21-26, 2000) 집적한다. 마이크로어레이어의 원리는 미세하게 제작된 핀이 DNA를 플레이트로부터 담아서 기판으로 컴퓨터가 지정한 똑같은 장소에 옮기는 것이다. 마이크로어레이어에 의해 옮겨진 탐침의 고정을 위해 45% 내지 65% 정도, 바람직하게는 50% 내지 55% 정도의 습도가 유지되는 조건에서 탐침의 3' 위치의 아민기와 유리판에 코팅되어 있는 알데히드기 사이의 반응이 원활이 이루어지도록 하기 위해 1시간 이상 반응을 유도하고, 6시간 이상 방치하여 DNA 탐침을 고정시킨다.Fixing and arranging different probes on a substrate surface uses methods such as pin microarrays, inkjets, photolithography, electrical arrays, and the like. In one embodiment of the present invention by using a microarrayer prepared according to a known method in a state in which the probe is dissolved in a buffer solution (Yoon et al, J. Microbiol. Biotechnol., 10 (1), 21- 26, 2000). The principle of microarrays is that the microfabricated pins carry DNA from the plate and transfer it to the same location specified by the computer on the substrate. Between the amine groups on the 3 'position of the probe and the aldehyde groups coated on the glass plate, under conditions where the humidity is maintained between 45% and 65%, preferably between 50% and 55%, for fixing the probe transferred by the microarray. In order to facilitate the reaction, the reaction is induced for at least 1 hour, and left for at least 6 hours to fix the DNA probe.

생존 유기체로부터 유래된 세포 또는 유기체 자체를 검출하기 위해 필요한 경우 이들 세포를 화학적 및/또는 물리적 방법으로 부분적으로 또는 완전히 용해시켜 그들 세포의 RNA 및/또는 DNA에 접근이 용이하게 만들고 본 발명의 탐침과 접촉시킨다. 접촉은 액체 매질 또는 용액중에서 니트로셀룰로즈, 셀룰로즈 또는 나일론 필터와 같은 적절한 지지체상에서 수행할 수 있다. 이러한 접촉은 최적 조건, 하위 최적 조건 또는 제한 조건하에서 일으킬 수 있다, 이러한 조건은 온도, 반응물 농도, 핵산의 염기쌍 최적 온도를 낮추는 물질 (예, 포름아미드, 디메틸설폭사이드 및 우레아)의 존재 및 반응 부피를 저감시키고/시키거나 하이브리드 형성을 가속하는 물질 (예, 덱스트란 설페이트, 폴리에틸렌글리콜 또는 페놀)의 존재를 포함한다.If necessary to detect cells derived from a living organism or the organism itself, these cells may be partially or completely lysed by chemical and / or physical methods to facilitate access to the RNA and / or DNA of those cells and Contact. The contact can be carried out on a suitable support such as nitrocellulose, cellulose or nylon filters in a liquid medium or solution. Such contact may occur under optimum conditions, suboptimal conditions or limit conditions, which conditions include the temperature, the reactant concentration, the presence of substances that lower the base pair optimal temperature of the nucleic acid (eg formamide, dimethylsulfoxide and urea) and the reaction volume. And / or the presence of a substance (eg, dextran sulfate, polyethyleneglycol or phenol) to reduce and / or accelerate hybrid formation.

탐침 제작Probe production

핵산 탐침은 통상의 클로닝 방법(Maniatis, T., et al. Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, New York, 1982)을 이용하여 목적하는 서열을 클로닝하거나 시판되는 DNA 합성기를 이용하여 화학적으로 합성하여 다량으로 얻을 수 있다.Nucleic acid probes can be cloned using conventional cloning methods (Maniatis, T., et al. Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, New York, 1982) or chemically using commercially available DNA synthesizers. It can be synthesized and obtained in large quantities.

본 발명의 탐침은 통상적인 방법에 따라 일본쇄 또는 이본쇄로 제작할 수 있다. 일본쇄의 탐침을 얻는 방법으로서 가장 대표적인 예는 자동화학합성기에서 DMT (dimethoxytrityl) 오프 (off) 방식에 의해 합성하여 탈보호 반응을 시행한 후, 원하는 수의 염기로 이루어진 탐침을 합성하는 것이다. 이렇게 제작된 탐침은 합성시 한 쪽 가닥 말단에 FITC (fluorescein isothiocyanate)와 같은 형광물질을 부착하여 특정 핵산의 존재유무를 확인할 수 있다. 다른 방식으로는 일본쇄의 DNA를 주형으로 하여 그와 상보적인 염기서열을 가지는 탐침을 만드는 방법으로, 주형의 DNA에 프라이머를 교잡시키고, 그 프라이머로부터 클레노우(Klenow) 효소와 형광물질이 부착된 염기(dNTP)를 사용하여 탐침을 합성하게 된다. 이는 DNA 내부에 형광물질이 부착하게 되므로, 높은 민감도 및 특이성을 갖는 탐침을 합성할 수 있다. 이중가닥의 탐침을 얻는 방법으로서, 게놈 DNA 혹은 플라스미드 DNA를 특정 제한효소로 절단하여 원하는 부분의 유전자 혹은 염기부분을 포함한 탐침을 얻을 수 있다. 랜덤 프라이밍(random priming) 방법은 6개의 랜덤 핵사머(random hexamer)와 주형 DNA를 혼성화시킨 후, 형광물질이 부착되어 있는 다양한 길이의 탐침을 합성하는 방법이고, 또 다른 방식으로는 DNA의 5'말단에 32P를 T4 폴리뉴클레오타이드 키나아제(polynucleotide kinase)를 이용하여 옮겨서 형광물질이 부착된 탐침을 합성할 수도 있으며, DNA 분해효소(DNase) I을 이용하여 이본쇄의 DNA에 절단부분을 만든 후, 이 DNA를 주형으로 하여 DNA 중합효소 I과 형광물질이 부착된 염기(dNTP)를 사용하여 탐침을 합성할 수 있다. 이렇게 이중가닥으로 합성된 탐침은 변성(denaturation)과정을 거쳐 일본쇄로 만든 후 혼성화 반응에 사용된다.The probe of the present invention can be produced in single strand or double strand according to a conventional method. As a method of obtaining a single chain probe, the most representative example is a synthesis by a DMT (dimethoxytrityl) off method in an automated synthesizer, subjected to a deprotection reaction, and then a probe composed of a desired number of bases. The probe thus prepared can be attached to a fluorescent substance such as fluorescein isothiocyanate (FITC) at one end of the strand to confirm the presence of a specific nucleic acid. Another method is to make a probe having a nucleotide sequence complementary to the DNA of a Japanese chain as a template. The primer is hybridized to the DNA of the template, and the Klenow enzyme and the fluorescent substance are attached from the primer. Base (dNTP) is used to synthesize the probe. Since the fluorescent material is attached to the inside of the DNA, it is possible to synthesize a probe having high sensitivity and specificity. As a method of obtaining a double-stranded probe, genomic DNA or plasmid DNA can be cut with a specific restriction enzyme to obtain a probe including a gene or a base portion of a desired portion. Random priming is a method of hybridizing six random hexamers and template DNA, and then synthesizing probes of various lengths to which fluorescent substances are attached. 32 P at the end of the T4 polynucleotide kinase (polynucleotide kinase) (transfer) can also be synthesized by the fluorescent probe attached, DNA cleavage (DNase) I using a DNA cleavage of the double strands, Using this DNA as a template, a probe can be synthesized using DNA polymerase I and a base (dNTP) to which a fluorescent substance is attached. The double-stranded probe is made into a Japanese chain after denaturation and used for hybridization.

본 발명의 탐침은 유리하게는 표지될 수 있다. 어떠한 통상의 표지도 사용할 수 있다. 탐침은 32P, 35S, 125I, 3H 및 14C와 같은 방사성 동위원소를 사용하여 표지할 수 있다. 방사성 표지는 3 또는 5 위치를 방사성 표지된 뉴클레오타이드, 폴리뉴클레오타이드 키나제, 말단 트랜스퍼라제 또는 리가제를 사용하여 말단 표지하는 것과 같은 통상적인 방법에 따라 수행할 수 있다. 방사성 표지의 다른 방법은 본 발명의 탐침을 화학적으로 요도드화하는 것이며 이러한 요오드화에 의해 탐침상에 수개의 125I 원자가 결합하게 된다. 이와 같이 본 발명의 탐침중 하나가 방사성으로 표지되면 일반적으로 자가방사기록, 액체 신틸레이션, 감마 계수 또는 다른 통상의 방법을 사용하여 방사선을 검출한다. 또한, 본 발명의 탐침은 면역 특성을 갖는 잔기(예, 항원 또는 합텐), 일부 시약에 대해 특이적 친화성을 갖는 잔기(예, 리간드), 검출가능한 효소 반응을 제공한 잔기(예, 효소, 보조효소, 효소 기질 또는 효소 반응에 참여하는 기질) 또는 어떤 파장에서 형광, 발광 또는 흡광의 물리적 특성을 제공하는 잔기과 결합하여 비방사성 표지될 수 있다. 또한, 탐침과 표적에 의해 형성된 하이브리드를 특이적으로 검출하는 항체를 사용할 수 있다. 비방사성 표지는 본 발명의 탐침을 화학적으로 합성할 때 제공할 수 있다. 아데노신, 구아노신, 시티딘, 티미딘 및 우라실 잔기는 다른 화학 잔기와 쉽게 결합하며 이에 탐침 또는 탐침과 상보적인 DNA 또는 RNA 단편사이에 형성된 하이브리드의 검출을 가능케 한다.Probes of the invention can be advantageously labeled. Any conventional label can be used. Probes can be labeled using radioactive isotopes such as 32 P, 35 S, 125 I, 3 H and 14 C. Radiolabeling can be carried out according to conventional methods such as end labeling the 3 or 5 position with radiolabeled nucleotides, polynucleotide kinases, terminal transferases or ligases. Another method of radiolabeling is to chemically iodide the probe of the present invention, which results in the binding of several 125 I atoms onto the probe. As such, when one of the probes of the present invention is labeled as radioactive, radiation is generally detected using autoradiography, liquid scintillation, gamma coefficients, or other conventional methods. In addition, the probes of the present invention may include residues having immune properties (eg, antigens or haptens), residues having specific affinity for some reagents (eg ligands), residues providing detectable enzymatic reactions (eg enzymes, Coenzymes, enzyme substrates or substrates participating in enzymatic reactions) or in combination with residues which provide physical properties of fluorescence, luminescence or absorption at certain wavelengths. Moreover, the antibody which specifically detects the hybrid formed by a probe and a target can be used. Non-radioactive labels can be provided when chemically synthesizing the probe of the present invention. Adenosine, guanosine, cytidine, thymidine and uracil residues readily bind to other chemical residues and allow detection of hybrids formed between the probe or probe and complementary DNA or RNA fragments.

표적Target

생물학적 시료에서 세균을 검출하고 동정하는데 사용하기 위한 핵산 기질을 제공하기 위해 시료로부터 핵산을 추출한다. 핵산은 표준 기술 또는 시판되고 있는 키트를 사용하여 다양한 시료로부터 추출할 수 있다. 예를 들면, 조직 시료로부터 RNA 또는 DNA를 분리하는데 사용하는 키트는 Qiagen, Inc. (미국 캘리포니아) 및 Stratagene (미국 캘리포니아)에서 구입할 수 있다. QIAAMP 블러드 키트는 혈액뿐만 아니라 골수, 체액 또는 세포 현탁액으로부터 DNA의 분리를 가능케 한다. QIAAMP 조직 키트는 근육 및 기관으로부터 DNA의 분리를 가능케 한다. 생물학적 시료가 목적하는 균주의 존재를 지시하는 rRNA 또는 rDNA를 함유하는 지의 여부를 결정하는 바람직한 방법으로 핵산을 균주세포로부터 음파 분쇄, 예를 들면 Murphy 등의 미국특허 제5,374,522호에 기술된 방법에 따라 방출시킬 수 있다. 세포를 분쇄하는 다른 공지 방법으로는 효소 사용, 삼투압 쇼크, 화학 처리 및 유리 비드와의 와동이 포함된다. 균주로부터 핵산을 방출시킬 수 있는 다른 적합한 방법이 Clark 등의 미국특허 제5,837,452호 및 Kacian 등의 미국특허 제5,364,763호에 기술되어 있다. rRNA의 방출 후 또는 방출과 동시에 표지된 탐침을 혼성화 촉진제의 존재하에 첨가하고 최적의 혼성화 온도에서 유의적인 혼성화 반응에 도달하는데 필요한 시간 동안 배양할 수 있다.The nucleic acid is extracted from the sample to provide a nucleic acid substrate for use in detecting and identifying bacteria in the biological sample. Nucleic acids can be extracted from various samples using standard techniques or commercially available kits. For example, kits for separating RNA or DNA from tissue samples may be found in Qiagen, Inc. (California, USA) and Stratagene (California, USA). The QIAAMP Blood Kit allows for the separation of DNA from blood as well as bone marrow, body fluids or cell suspensions. The QIAAMP Tissue Kit allows for the separation of DNA from muscles and organs. As a preferred method of determining whether a biological sample contains rRNA or rDNA indicating the presence of the desired strain, nucleic acid is sonicated from strain cells, for example according to the method described in US Pat. No. 5,374,522 to Murphy et al. Can be released. Other known methods of crushing cells include enzyme use, osmotic shock, chemical treatment, and vortexing with glass beads. Other suitable methods for releasing nucleic acids from strains are described in US Pat. No. 5,837,452 to Clark et al. And US Pat. No. 5,364,763 to Kacian et al. Labeled probes may be added after or concurrent with release of the rRNA in the presence of a hybridization promoter and incubated for the time necessary to reach a significant hybridization reaction at the optimal hybridization temperature.

표적 핵산이 이본쇄인 경우에는 검출 과정을 이행하기 이전에 변성을 실시하는 것이 바람직하다. 이본쇄 핵산의 변성은 화학적, 물리적 또는 효소적 변성의 공지 방법, 특히 적절한 온도, 80℃ 이상의 온도로 가열함으로써 수행할 수 있다.If the target nucleic acid is double stranded, it is preferable to perform denaturation before performing the detection process. Modification of the double-stranded nucleic acid can be carried out by known methods of chemical, physical or enzymatic modification, in particular by heating to an appropriate temperature, a temperature of at least 80 ℃.

또한, 탐침과 혼성화 반응을 하는 표적 DNA는 보통 두 종류의 방법으로 준비될 수 있다. 첫째는, 서던 블럿 또는 노던 블럿시 사용되는 방법으로, 게놈 DNA 혹은 플라스미드 DNA를 적당한 제한효소로 자른 후 아가로스 겔(agarose gel) 상에서 전기영동을 하여 크기별로 DNA 조각을 분리하여 사용한다. 둘째는, PCR 방법을 이용하여 원하는 부분의 DNA를 증폭시켜 사용하는 방법이다. 이때, 사용하는 PCR 방법을 살펴보면, 전방향과 역방향의 프라이머 쌍을 동일한 양을 사용해서 증폭시키는 가장 보편화되어 있는 PCR과 전방향과 역방향의 프라이머 쌍을 비대칭적으로 첨가시켜서 2중 가닥과 단일 가닥의 밴드를 동시에 얻을 수 있는 비대칭 증폭 방법(Asymmetric PCR), 여러 가지의 프라이머 쌍을 넣어서 한꺼번에 증폭시킬 수 있는 다중 증폭 방법(Multiplex PCR), 특이적인 4개의 프라이머와 리가아제(Ligase)를 이용해 증폭한 후 효소면역법으로 형광량을 판정하는 리가아제 연쇄반응(Ligase Chain Reaction; LCR)방법, 이 밖에도 핫스타트 PCR, 네스트 PCR, 변성 올리고뉴클레오티드 프라이머 PCR, 역전사 효소 PCR, 반-정량적(Semi-quantitative) 역전사 효소 PCR, 실시간 PCR(Real time PCR), SACE(Rapid Amplification of cDNA Ends), 경쟁적 PCR(Competitive PCR), 연쇄반복(short tandem repeats; STR), 단일가닥입체다형화(Single Strand Conformation Polymorphism; SSCP), 동소 PCR, DDRT-PCR(Differential Display Reverse Transcriptase PCR) 등의 방법 등이 있다.In addition, the target DNA that hybridizes with the probe may be prepared by two kinds of methods. First, a method used in Southern blot or Northern blot, genomic DNA or plasmid DNA is cut with a suitable restriction enzyme and subjected to electrophoresis on an agarose gel to separate DNA fragments by size. The second method is to amplify and use DNA of a desired portion using a PCR method. In this case, using the PCR method, the most common PCR that amplifies the forward and reverse primer pairs using the same amount and the asymmetric addition of the forward and reverse primer pairs of the double strand and the single strand Asymmetric PCR (Asymmetric PCR) to obtain bands at the same time, Multiple amplification method (Multiplex PCR) that can be amplified at the same time by putting several primer pairs, After amplification using four specific primers and Ligase (Ligase) Ligase Chain Reaction (LCR) method to determine the amount of fluorescence by enzyme immunoassay, as well as hot start PCR, nest PCR, modified oligonucleotide primer PCR, reverse transcriptase PCR, semi-quantitative reverse transcriptase PCR, Real time PCR, Rapid Amplification of cDNA Ends (SACE), Competitive PCR, Short tandem repeats (S) TR), Single Strand Conformation Polymorphism (SSCP), in situ PCR, and DDRT-PCR (Differential Display Reverse Transcriptase PCR).

특정 PCR 산물의 증폭을 위한 주형으로서 비교적 균질한 시료(예, 혈액, 세균 콜로니 또는 뇌척수액)가 보다 복합적인 시료(예, 뇨, 타액 또는 배설물)보다 더 적합한 것으로 알려져 있다(Shibata in PCR:The Polymerase Chain Reaction, Mullis et al., eds., Birkhauser, Boston (1994), pp, 47-54). 뇌척수액과 같이 증폭하고자 하는 표적 핵산의 복제물이 비교적 적게 함유된 시료는 PCR에 직접 첨가할 수 있다. 혈액 시료는 적혈구의 억제 특성으로 인해 PCR시 특별히 다루어야 하는데 PCR에 혈액을 사용하기 전에 적혈구 세포를 제거해야 한다. 이 목적을 위해 많은 방법이 이용되고 있으며, 예를 들면 CHELEX 100 컬럼(BioRad) 등을 통과시키는 것과 QIAAMP 블러드 키트를 사용하는 것들이 포함된다. 타액으로부터 핵산을 추출하는 것은 목적하는 균외의 다른 세균 종을 사멸시키거나 성장을 억제하는 사전 정화 과정이 필요하다. 이러한 정화 과정은 시료를 N-아세틸-L-시스테인 및 NaOH로 처리함으로써 달성된다. 이러한 정화과정은 타액 시료를 분석하기 전에 배양할 때만이 필요하다.As a template for the amplification of certain PCR products, relatively homogeneous samples (e.g. blood, bacterial colonies or cerebrospinal fluid) are known to be more suitable than more complex samples (e.g. urine, saliva or excreta) (Shibata in PCR: The Polymerase). Chain Reaction, Mullis et al., Eds., Birkhauser, Boston (1994), pp, 47-54). Samples containing relatively few copies of the target nucleic acid to be amplified, such as cerebrospinal fluid, can be added directly to the PCR. Blood samples should be specially handled during PCR due to the inhibitory properties of red blood cells, and red blood cells should be removed before using blood for PCR. Many methods are used for this purpose, including for example passing through a CHELEX 100 column (BioRad) and the like using the QIAAMP blood kit. Extracting nucleic acids from saliva requires a preliminary purification process that kills or inhibits growth of other bacterial species other than the desired organism. This purification process is accomplished by treating the sample with N-acetyl-L-cysteine and NaOH. This purification process is only necessary when incubating the saliva sample prior to analysis.

본 발명의 바람직한 양태는 분리된 검체의 DNA를 주형으로 하고 비대칭 증폭 방법(Asymmetric PCR)을 수행하여 단편 유전자를 제작한다. 단편 유전자는 전방향 프라이머와 역방향 프라이머의 첨가비율을 1:5로 차별화 함으로써 한번의 중합효소연쇄반응으로 획득한다. 이때 사용한 프라이머는 세균의 경우 공통적으로 16S rRNA내지 23S rRNA에 존재하는 염기 서열 부분으로(Pirkko K. et al., Clin. Micorbiol., 36(8), 2205-2209, 1999) 다음과 같다:In a preferred embodiment of the present invention, a fragment gene is prepared by using DNA of an isolated sample as a template and performing asymmetric amplification (Asymmetric PCR). The fragment gene is obtained by one polymerase chain reaction by differentiating the ratio of forward and reverse primers at 1: 5. In this case, the primers used are part of a nucleotide sequence existing in 16S rRNA to 23S rRNA in common in bacteria (Pirkko K. et al., Clin. Micorbiol., 36 (8), 2205-2209, 1999).

프라이머 1 (센스): TTGTACACACCGCCCGTC (서열번호 406, 1585Fw)Primer 1 (sense): TTGTACACACCGCCCGTC (SEQ ID NOs: 406, 1585Fw)

프라이머 2 (안티센스): F-TTCGCCTTTCCCTCACGGTACT (서열번호 407, 23BR);Primer 2 (antisense): F-TTCGCCTTTCCCTCACGGTACT (SEQ ID NOs: 407, 23BR);

프라이머 3 (센스): AGTACCGTGAGGGAAAGGGGAA (서열번호 408, 23BFw)Primer 3 (sense): AGTACCGTGAGGGAAAGGGGAA (SEQ ID NOs: 408, 23BFw)

프라이머 4 (안티센스): F-TGCTTCTAAGCCAACATCCT (서열번호 409, 37R); 및Primer 4 (antisense): F-TGCTTCTAAGCCAACATCCT (SEQ ID NOs: 409, 37R); And

프라이머 5 (센스): AGGATGTTGGCTTAGAAGCA (서열번호 410, MS37F)Primer 5 (Sense): AGGATGTTGGCTTAGAAGCA (SEQ ID NO: 410, MS37F)

프라이머 6 (안티센스): F-CCCGACAAGGAATTTCGCTACCTT (서열번호 411, MS38R).Primer 6 (antisense): F-CCCGACAAGGAATTTCGCTACCTT (SEQ ID NO: 411, MS38R).

이들 프라이머에 대한 대략 위치도는 도 1과 같고 F는 5' 말단에 부착된 FITC 형광물질을 가리킨다. PCR 수행시 탐침과 결합한 DNA를 확인하기 위해 5-FITC가 부착된 프라이머를 이용하여 증폭함으로써 형광을 통해 결합을 확인함으로써 감염 여부와 감염균의 종류를 알 수 있도록 한다. 또한, 이들 프라이머로 증폭할 수 없는 부분을 얻기 위해 바이오인포메틱스 (Bioinformatics) 방법으로 다중정렬, BLAST를 실시하여 프라이머를 디자인한다.Approximate location maps for these primers are shown in FIG. 1 and F indicates the FITC fluorescent material attached to the 5 'end. In order to identify DNA bound to the probe during PCR, amplification using a 5-FITC attached primer is performed to confirm the binding through fluorescence so that the infection can be identified and the type of infection. In addition, in order to obtain a portion that cannot be amplified by these primers, primers are designed by performing multi-alignment and BLAST by a bioinformatics method.

진균의 경우 사용한 프라이머는 진균류의 18S rRNA에 존재하는 염기 서열 부분으로 본 발명자가 직접 디자인하였고 염기서열은 다음과 같다:In the case of fungi, the primers used were part of the nucleotide sequence present in the 18S rRNA of the fungus and designed by the present inventors.

프라이머 1(센스): GTAATTGGAATGAGTACAAT(서열번호 412, fun463F)와 Primer 1 (sense): GTAATTGGAATGAGTACAAT (SEQ ID NO: 412, fun463F)

프라이머 2(안티센스): F-CTACGACGGTATCTGATCAT(서열번호 413, fun986R).Primer 2 (antisense): F-CTACGACGGTATCTGATCAT (SEQ ID NO: 413, fun986R).

본 발명의 바람직한 양태로서 PCR 반응은 10X PCR 완충액(100mM Tris-HCl(pH8.3), 500mM KCl, 15mM MgCl2) 5ul, dNTP 혼합물(dATP, dGTP, dCTP, dTTP 각각 2.5mM) 4ul, 10pmole 전방향 프라이머 0.5ul, 10pmole 역방항 프라이머 2.5ul, 1/10로 희석한 주형DNA(100ng) 1ul, Taq 폴리머라제(5unit/ul, Takara Shuzo Co., Shiga, Japan) 0.5ul를 첨가후 총 부피가 50ul가 되도록 물을 첨가한다. 첫 번째 변성은 94℃에서 7분간, 두 번째 변성은 94℃에서 1분간 수행한다. 교잡(annealing)은 52℃에서 1분, 연장(extension)은 72℃에서 1분간 수행하며, 이를 10회 반복한다. 그 후, 다시 세 번째 변성은 94℃에서 1분, 교잡은 54℃에서 1분, 연장은 72℃ 에서 1분간 수행하며, 이를 30회 반복한다. 이후 72℃에서 5분간 마지막 연장을 1회 수행한다. PCR 반응결과로 생성된 산물은 아가로스 겔 전기영동법(agarose gel electrophoresis)으로 확인한다.As a preferred embodiment of the present invention, the PCR reaction was performed in 10X PCR buffer (100 mM Tris-HCl (pH8.3), 500 mM KCl, 15 mM MgCl 2 ) 5ul, dNTP mixture (dATP, dGTP, dCTP, dTTP 2.5mM) 4ul, 10pmole before Total volume after adding 0.5ul aromatic primer, 2.5ul 10pmole reverse primer, 1ul template DNA (100ng) diluted with 1/10, 0.5ul Taq polymerase (5unit / ul, Takara Shuzo Co., Shiga, Japan) Add water to 50ul. The first denaturation is carried out at 94 ° C. for 7 minutes and the second denaturation at 94 ° C. for 1 minute. Annealing is performed at 52 ° C. for 1 minute and extension at 72 ° C. for 1 minute, and this is repeated 10 times. Thereafter, the third denaturation was performed at 94 ° C for 1 minute, hybridization at 54 ° C for 1 minute, extension at 72 ° C for 1 minute, and this was repeated 30 times. Thereafter, the last extension was performed once for 5 minutes at 72 ° C. The product produced by the PCR reaction is confirmed by agarose gel electrophoresis.

혼성화 및 세척Hybridization and Washing

혼성화 기술은 본 발명에서 특정적인 것이 아니다. 이 기술은 일반적으로 문헌(Nucleic Acid Hybridization: A Practical Approach, Ed. Hames, B. D. and Higgins, S. J., IRL Press, 1987; Gall and Pardue (1969), Proc. Natl. Acad. Sci., U.S.A., 63:378-383, and John, Burnsteil and Jones (1969) Nature, 223:582-587)에 기술되어 있다.Hybridization techniques are not specific to the present invention. This technique is generally described in Nucleic Acid Hybridization: A Practical Approach, Ed. Hames, BD and Higgins, SJ, IRL Press, 1987; Gall and Pardue (1969), Proc. Natl. Acad. Sci., USA, 63: 378-383, and John, Burnsteil and Jones (1969) Nature, 223: 582-587).

혼성화 조건은 긴축(stringency), 즉 작용 조건의 엄격함에 의해 결정된다. 혼성화가 이루어지는 긴축이 고도하면 할수록 혼성화는 더욱 특이적이 된다. 긴축은 특히 탐침/표적 결합체의 염기 조성뿐만 아니라 두 핵산사이의 부정합 정도의 함수이다. 긴축은 또한 혼성화 용액에 존재하는 이온의 농도 및 종류, 변성제의 성질 및 농도 및/또는 혼성화 온도와 같은 혼성화 반응 변수의 함수일 수 있다. 혼성화 반응이 수행되어야 하는 조건의 기축은 특히 사용된 탐침에 의존한다. 모든 이들 데이터는 잘 알려져 있으며 적절한 조건은 개개의 경우에 통상의 실험에 의해 결정할 수 있다. 일반적으로, 사용된 탐침의 길이에 따라 혼성화 반응의 온도는 약 0.8 내지 1 M 농도의 염수 용액중에서 약 20℃ 내지 65℃, 특히 35℃ 내지 65℃이다.Hybridization conditions are determined by stringency, i.e. the stringency of the operating conditions. The higher the tightening that hybridization takes place, the more specific the hybridization becomes. Constriction is a function of not only the base composition of the probe / target conjugate but also the degree of mismatch between the two nucleic acids. Constriction may also be a function of hybridization reaction variables such as the concentration and type of ions present in the hybridization solution, the nature and concentration of the denaturant and / or the hybridization temperature. The basis of the conditions under which the hybridization reaction should be carried out depends in particular on the probe used. All these data are well known and the appropriate conditions can be determined by routine experimentation in the individual case. Generally, depending on the length of the probe used, the temperature of the hybridization reaction is about 20 ° C. to 65 ° C., in particular 35 ° C. to 65 ° C., in a saline solution at a concentration of about 0.8 to 1 M.

표지된 올리고뉴클레오타이드 탐침과 표적 핵산사이의 혼성화는 Hogan 등의 미국특허 제5,030,557호에 기술된 절차에 따라 비표지된 헬퍼올리고뉴클레오타이드를 사용하여 증가시킬 수 있다. 헬퍼올리고뉴클레오타이드는 탐침에 의해 결합되는 영역외의 다른 표적 핵산의 영역과 결합한다. 이 결합은 일본쇄 핵산의 표적 영역에 새로운 이차 및 삼차 구조를 취해서 탐침의 결합 속도를 가속화한다.Hybridization between the labeled oligonucleotide probe and the target nucleic acid can be increased using unlabeled helper oligonucleotides following the procedure described in US Pat. No. 5,030,557 to Hogan et al. The helper oligonucleotide binds to a region of the target nucleic acid other than the region bound by the probe. This binding takes on new secondary and tertiary structures in the target region of single-stranded nucleic acids, accelerating the binding speed of the probe.

당업자는 탐침과 표적사이에 형성된 하이브리드의 열 안정성에 영향을 미치는 인자들 또한 탐침 특이성에 영향을 미칠 수 있음을 이해할 것이다. 따라서, 탐침과 표적의 하이브리드의 융점 온도를 포함하여 융점 프로필이 각각의 탐침과 표적의 하이브리드에 대해 경험적으로 결정되어야 한다. 이러한 결정은 Arnold 등의 미국특허 제5,283,174호에 기술된 방법에 따라 수행할 수 있다. 탐침과 표적의 하이브리드의 융점 온도를 결정하는 한 가지 방법은 혼성화 보호 검정을 수행하는 것으로 포함한다. 이 검정 방법에 따르면 리튬 라우릴설페이트를 함유한 리튬 석시네이트 완충 용액중의 과량의 표적의 조건하에서 탐침과 표적의 하이브리드를 형성한다. 미리 형성된 하이브리드의 일부를 혼성화 완충액에 희석하고 예상되는 융점 온도 (전형적으로 55℃) 이하에서 시작하여 2 내지 5℃를 증가시킨 여러 온도에서 5분 동안 배양한다. 그런 후 이 용액을 약알카리성 보레이트 완충액으로 희석하고 보다 낮은 온도 (예, 50℃)에서 10분동안 배양한다. 일본쇄 탐침에 연결된 아크리디늄 에스테르는 이들 조건하에서 가수분해되는 한편 혼성화된 탐침에 연결된 아크리디늄 에스테르는 상대적으로 보호된다. 이 절차를 혼성화 보호 검정이라고 한다. 남아 있는 화학발광의 양은 하이브리드의 양과 비례하며 과산화수소에 이어 알카리를 차례로 첨가하여 발광계측기로 측정한다. 이 데이터를 온도에 대한 최대 시그날 (보통 최저 온도)의 퍼센트로 점적한다. 융점 온도는 최대 시그날의 50%가 남아 있는 포인트로서 정의된다. 다른 방도로서, 탐침과 표적의 하이브리드에 대한 융점 온도는 방사성 동위원소로 표지된 탐침을 이용하여 결정할 수 있다. 모든 경우에 있어서, 주어진 하이브리드에 대한 융점 온도는 혼성화 용액에 함유된 염, 세정제 및 기타 용질의 농도에 의해 다양할 수 있다. 이들 모든 인자는 열변성 동안에 상대적인 하이브리드 안정성에 영향을 미친다(Molecular Cloning: A Laboratory Manual Sambrook et al., eds. Cold Spring Harbor Lab Publ., 9.51 (1989)).Those skilled in the art will appreciate that factors affecting the thermal stability of the hybrid formed between the probe and the target may also affect probe specificity. Therefore, the melting point profile, including the melting point temperature of the probe and target hybrid, must be determined empirically for each probe and hybrid of the target. This determination can be made according to the method described in US Pat. No. 5,283,174 to Arnold et al. One method of determining the melting point temperature of the probe and target hybrids involves performing hybridization protection assays. This assay method forms a hybrid of the probe with the target under conditions of excess target in a lithium succinate buffer solution containing lithium laurylsulfate. A portion of the preformed hybrid is diluted in hybridization buffer and incubated for 5 minutes at various temperatures starting at or below the expected melting point temperature (typically 55 ° C.) and increasing 2 to 5 ° C. The solution is then diluted with weakly alkaline borate buffer and incubated at lower temperature (eg 50 ° C.) for 10 minutes. The acridinium esters linked to single chain probes are hydrolyzed under these conditions while the acridinium esters linked to hybridized probes are relatively protected. This procedure is called hybridization protection assay. The amount of chemiluminescence remaining is proportional to the amount of hybrid and is measured with a luminometer by adding hydrogen peroxide followed by alkali. This data is dipped as a percentage of the maximum signal (usually the lowest temperature) to the temperature. The melting point temperature is defined as the point at which 50% of the maximum signal remains. Alternatively, the melting point temperature for the hybrid of the probe and the target can be determined using a probe labeled with a radioisotope. In all cases, the melting point temperature for a given hybrid may vary by the concentration of salts, detergents and other solutes contained in the hybridization solution. All these factors affect the relative hybrid stability during heat denaturation (Molecular Cloning: A Laboratory Manual Sambrook et al., Eds. Cold Spring Harbor Lab Publ., 9.51 (1989)).

혼성화 조건은 몇 가지의 변수, 예를 들면 혼성화 온도, 매질 성분의 성질 및 농도, 형성된 하이브리드와 세척시 온도 등을 고려하여 모니터링할 수 있다. 혼성화 및 세척 온도는 탐침의 염기 서열, 종류 및 길이에 따라 상위 값으로 한정하며 최대 혼성화 또는 세척 온도는 약 30℃ 내지 60℃ 이다. 보다 높은 온도에서 혼성화와 탐침과 표적사이에 형성된 하이브리드의 해리 또는 변성과 경쟁한다. 혼성화 매질은 예를 들면 약 3xSSC (1xSSC=0.15 M NaCl, 0.015 M 시트르산나트륨, pH 7.0), 약 25 mM 인산염 완충액 PH 7.1 및 20% 탈이온 포름아미드, 0.02% 피콜, 0.02% 소혈청 알부민, 0.02% 폴리비닐피롤리돈 및 약 0.1 mg/ml 절삭되고 변성된 연어 정자 DNA를 함유한다. 세척 매질은 예를 들면 약 3xSSC, 25 mM 인산염 완충액 pH 7.1 및 20% 탈이온 포름아미드를 함유한다. 탐침 및 매질의 변형에 따라, 목적하는 특이성을 얻기 위해 혼성화 또는 세척 온도는 알려진 연관성에 따라 바꾸어 주어야 한다(B. D. HAMES and S. J. HIGGINS, (eds.). Nucleic acid hybridzation. A practical approach, IRL Press, Oxford, U.K., 1985). 이러한 관점에서, 일반적으로 DNA:DNA 하이브리드는 RNA:DNA 또는 RNA:RNA 하이브리드보다 덜 안정하기 때문에, 검출될 하이브리드의 성질에 따라 혼성화 조건은 특이적 검출을 달성하기 위해 적절히 조정되어야 한다.Hybridization conditions can be monitored taking into account several variables, for example, the hybridization temperature, the nature and concentration of the media components, the hybrids formed and the temperature upon washing. Hybridization and wash temperatures are limited to higher values depending on the base sequence, type and length of the probe and the maximum hybridization or wash temperature is about 30 ° C. to 60 ° C. At higher temperatures it competes with hybridization and dissociation or denaturation of the hybrid formed between the probe and the target. Hybridization media are for example about 3xSSC (1xSSC = 0.15 M NaCl, 0.015 M sodium citrate, pH 7.0), about 25 mM phosphate buffer PH 7.1 and 20% deion formamide, 0.02% picol, 0.02% bovine serum albumin, 0.02 % Polyvinylpyrrolidone and about 0.1 mg / ml cut and denatured salmon sperm DNA. The wash medium contains, for example, about 3 × SSC, 25 mM phosphate buffer pH 7.1 and 20% deion formamide. Depending on the modification of the probe and medium, the hybridization or washing temperature should be changed according to known associations to achieve the desired specificity (BD HAMES and SJ HIGGINS, (eds.). Nucleic acid hybridzation.A practical approach, IRL Press, Oxford , UK, 1985). In this regard, since DNA: DNA hybrids are generally less stable than RNA: DNA or RNA: RNA hybrids, hybridization conditions must be appropriately adjusted to achieve specific detection, depending on the nature of the hybrid to be detected.

본 발명의 바람직한 특정 양태로서, 혼성화 완충용액(6×SSPE (0.15M NaCl, 5mM C6H5Na3O7, pH 7.0), 20% (v/v) 포름아미드)을 PCR 증폭 유전자와 혼합하고 탐침이 고정된 유리판 위에 분주한 후 30℃에서 6시간 동안 반응시켜 상보적인 결합을 유도한다. 시간이 경과한 후 3×SPE, 2×SSPE, 1×SSPE 순으로 각각 5분씩 세척한다.In a particular preferred embodiment of the invention, hybridization buffer (6 × SSPE (0.15 M NaCl, 5 mM C 6 H 5 Na 3 O 7 , pH 7.0), 20% (v / v) formamide) is mixed with a PCR amplification gene After dispensing the probe on a fixed glass plate it is reacted for 6 hours at 30 ℃ to induce complementary binding. After the passage of time, wash for 3 minutes in the order of 3 × SPE, 2 × SSPE, 1 × SSPE.

하이브리드의 정량은 통상적인 방법에 따라 표적을 상기된 탐침에서와 같이 형광 혹은 방사성 동위원소로 표지하여 이루어 질 수 있다. 표지는 프라이머 자체에 할 수도 있고 증폭 및 전사시에 형광 및 방사성 동위원소가 표지된 염기 잔기를 사용함으로써 이루어질 수 있다.Quantification of the hybrid can be accomplished by labeling the target with fluorescent or radioisotopes, as in the probes described above, according to conventional methods. Labeling may be on the primer itself or by using base residues labeled with fluorescent and radioisotopes for amplification and transcription.

진단 용도Diagnostic purpose

본 발명의 탐침은 키트, 특히 DNA 칩에 한종으로 적용하여 해당 균을 고도의 특이성으로 검출함으로서 각 균에 의해 유발되는 감염 질환을 단독으로 정확하게 진단할 수 있거나 2종 이상의 탐침을 적용함으로써 2종 이상의 감염 질환 원인균을 고도의 특이성으로 검출함으로서 그들 균에 의해 유발되는 감염 질환을 동시에 정확하게 진단할 수 있다. 본 발명에 따른 균에 의해 유발되는 감염 질환은 많은 문헌을 통해 잘 알려져 있으며 예를 들면 다음과 같다:The probe of the present invention can be precisely diagnosed solely and accurately by infecting diseases caused by each bacterium by detecting the bacterium with high specificity by applying one species to a kit, especially a DNA chip, or by applying two or more probes. By detecting the infectious disease-causing bacteria with a high specificity, it is possible to accurately diagnose the infectious disease caused by these bacteria at the same time. Infectious diseases caused by the bacterium according to the present invention are well known in the literature, for example:

아시네토박터 바우마니 균은 인체의 모든 장기에 화농성 감염(Glew RH. et al., Medicine (Baltimore). 56, 79-97, (1977)), 요도관이나 요로 결석과 관련되어 신우신염과 방광염(Glew RH et al., Medicine (Baltimore), 56, 79-97, (1977)), 뇌막염(Berk SL et al., Arch. Neurol. 38, 95-98, (1981)), 봉와직염(Gervich DH et al, Am. J. Infect. Control, 13, 210-215, (1985)), 창상감염(Tong MJ. JAMA. 219, 1044-1047, (1972)), 괴사성 근막염(Amsel MB et al., Curr. Surg. 42, 370-372, (1985)), 내안구염 (Peyman GA et al., Am. J. Ophthalmol. 80, 764-765, (1975)), 심내막염 (Gradon JD, et al., Clin. Infect. Dis. 14, 1145-1148, (1992)), 골수염, 세균성 관절염, 간농양, 췌장 농양(Henricksen SD. Bacteriol. Rev. 37, 522-561, (1973)) 등을 일으키고;Acinetobacter Baumani is associated with pyelonephritis and cystitis associated with urinary tract or urinary stones in all organs of the body (Glew RH. Et al., Medicine (Baltimore). 56, 79-97, (1977)). (Glew RH et al., Medicine (Baltimore), 56, 79-97, (1977)), meningitis (Berk SL et al., Arch. Neurol. 38, 95-98, (1981)), cellulitis (Gervich DH et al, Am. J. Infect. Control, 13, 210-215, (1985)), wound infection (Tong MJ. JAMA. 219, 1044-1047, (1972)), necrotizing fasciitis (Amsel MB et al. , Curr. Surg. 42, 370-372, (1985)), endophthalmitis (Peyman GA et al., Am. J. Ophthalmol. 80, 764-765, (1975)), endocarditis (Gradon JD, et al. , Clin. Infect. Dis. 14, 1145-1148, (1992)), osteomyelitis, bacterial arthritis, liver abscess, pancreatic abscess (Henricksen SD. Bacteriol. Rev. 37, 522-561, (1973)), and the like;

언에어로비오스피리룸 숙시니시프로더센스 균은 임상적으로는 주로 균혈증(Tee W et al., J. Clin. Microbiol. 36(5), 1209-1213, (1998)), 패혈증(Marcus L et al., Eur. J. Clin. Microbiol. Infect. Dis. 15(9), 741-744, (1996)), 설사(Malnick H et al., J. Clin. Pathol., 36(10), 1097, (1983) 등을 일으키고;Unaerobiopyrirum succinicis prodersense is clinically mainly composed of bacteremia (Tee W et al., J. Clin. Microbiol. 36 (5), 1209-1213, (1998)), sepsis (Marcus L et. al., Eur. J. Clin.Microbiol.Infect.Dis. 15 (9), 741-744, (1996)), diarrhea (Malnick H et al., J. Clin. Pathol., 36 (10), 1097 , (1983) and the like;

박테로이데스 프라질리스 균은 중추신경계에서 뇌막염(Feder HM. Rev. Infect. Dis. 9, 783-786, (1987)), 뇌농양, 경막하축농 또는 경막외 농양(Swartz MN. In: Finegold SM, George WL, eds. Anaerobic infections in humans. New York: Academic; 155-212, 1989), 만성 부비동염 (Frederick J et al., N. Engl. J. Med. 290, 135-137, (1974)), 복강 내 감염(Gorbach SL. Clin. Infect. Dis. 17, 961-967, (1993)), 간농양(Rubin RH et al., Am. J. Med. 57, 601-610, (1974)), 균혈증(Lombardi DP et al., Am. J. Med, 92, 53-60, (1992); Chow AW. et al., Medicine (Baltimore) 53, 93-123, (1974); 및 Redondo MC et al., Clin. Infect. Dis., 20, 1492-1496, (1995)), 심내막염(Felner JM et al., N. Engl. J. Med. 282, 1188-1192, (1970); Nastro LJ et al., Am. J. Med., 54, 482-496, (1973); 및 (Nastro LJ et al., Am. J. Med., 54, 482-496, (1973)), 창상 감염, 개와 사람으로 물린 창상, 괴사성 근막염, 당뇨병성 괴양 또는 봉와직염(Gerding D. Clin. Infect. Dis. 20(Suppl 2), S283-S288, (1995)), 만성 골수염, 세균성 관절염(Rosenkranz P et al., Rev. Infect. Dis. 12, 20-30, (1990)을 일으키고;Bacterides praxilis is known as meningitis in the central nervous system (Feder HM. Rev. Infect. Dis. 9, 783-786, (1987)), cerebral abscesses, subdural sheaths or epidural abscesses (Swartz MN. In: Finegold SM, George WL, eds.Anaerobic infections in humans.New York: Academic; 155-212, 1989), chronic sinusitis (Frederick J et al., N. Engl. J. Med. 290, 135-137, (1974)), Intraperitoneal infection (Gorbach SL. Clin. Infect. Dis. 17, 961-967, (1993)), liver abscess (Rubin RH et al., Am. J. Med. 57, 601-610, (1974)), bacteremia (Lombardi DP et al., Am. J. Med, 92, 53-60, (1992); Chow AW. Et al., Medicine (Baltimore) 53, 93-123, (1974); and Redondo MC et al. , Clin. Infect. Dis., 20, 1492-1496, (1995)), endocarditis (Felner JM et al., N. Engl. J. Med. 282, 1188-1192, (1970); Nastro LJ et al. , Am. J. Med., 54, 482-496, (1973); and (Nastro LJ et al., Am. J. Med., 54, 482-496, (1973)), wound infections, dogs and humans. Bite wound, necrotizing fasciitis, diabetic ulcer or cellulitis (Gerding D. Clin. t. Dis. 20 (Suppl 2), S283-S288, (1995)), chronic osteomyelitis, bacterial arthritis (Rosenkranz P et al., Rev. Infect. Dis. 12, 20-30, (1990);

카디오박테리움 호미니스 균은 심내막염(Traveras J.Md. et al., South. Med. J., 86, 1439-1440, (1993)), 합병증으로 전신에 색전증 및 뇌에 동맥류, 심부전을 일으키고;Cardiobacterium hominis bacteria are endocarditis (Traveras J. Md. Et al., South. Med. J., 86, 1439-1440, (1993)), complications causing embolism and aneurysms and heart failure in the systemic body;

크리서박테리움 메닌고셉티쿰 신생아에서 뇌막염(Plotkin S.A. et al., JAMA. 198, 194-196, (1966); Pokrywka M. et al., Am. J. Infect. Control, 21, 139-145, (1993)), 호흡기 감염(Brown R.B. et al., Am. J. Infect. Control, 17, 121-125, (1989)), 패혈증, 심내막염, 봉와직염, 창상 감염, 복강 농양, 복막염, 내안구염(Olsen H. et al., Lancet, 1, 1294-1296, (1965); Sheridan R.L. et al., Clin. Infect. Dis., 17, 185-187, (1993)) 등을 일으키고;Meningitis in newborns of Chrysbacterium meningocystum (Plotkin SA et al., JAMA. 198, 194-196, (1966); Pokrywka M. et al., Am. J. Infect.Control, 21, 139-145, (1993)), respiratory infections (Brown RB et al., Am. J. Infect. Control, 17, 121-125, (1989)), sepsis, endocarditis, cellulitis, wound infection, celiac abscess, peritonitis, endophthalmitis ( Olsen H. et al., Lancet, 1, 1294-1296, (1965); Sheridan RL et al., Clin. Infect. Dis., 17, 185-187, (1993)) and the like;

클로스트리디움 람모섬 균은 염증성 장 질환(Senda S, et al., Microbial Immunol 1985;29(11):1019-28), 뇌농양(An Med Interna. 1998 Jul;15(7):392-3), 신장이식을 받은 환자에게 폐혈성 동맥성 색전 (Transplant Proc. 1983 Jun;15(2):1715-9) 등을 일으키고;Clostridium rhammosome is an inflammatory bowel disease (Senda S, et al., Microbial Immunol 1985; 29 (11): 1019-28), brain abscess (An Med Interna. 1998 Jul; 15 (7): 392-3) Pulmonary arterial embolism (Transplant Proc. 1983 Jun; 15 (2): 1715-9), etc. in patients undergoing kidney transplantation;

코마모나스 아시도보란스 균은 약물 남용자에게 심내막염(Horowitz H. et al., J. Clin. Microbiol., 28, 143-145, (1990))을 일으키고;Comamonas asidobarans causes endocarditis in drug abusers (Horowitz H. et al., J. Clin. Microbiol., 28, 143-145, (1990));

코리네박테리움 디프테리애 균은 호흡기 디프테리아(Dobie RA, et al., JAMA. 1979;242:2197-2201), 심근염(Boyer NH, et al., N Engl J Med. 1948;239:913.), 안구운동과 섬모의 마비(Kallick CA, et al., III Med J. 1970;137:505-512; 및 Naiditch MJ, et al., Am J Med. 1954;17:229-245), 얼굴과 인두 그리고 후두의 기능 장애, 말초신경염, 피부 디프테리아 (Koopman JS, et. al., J Infect Dis. 1975;131:239-244), 심내막염(Tiley SM, et al., Clin Infect Dis. 1993;16:271-275), 진균 동맥류(Gruner E, et al., Clin Infect Dis. 1994;18:94-96), 골수염(Patey O, et al., J Clin Microbiol. 1997;35:441-445) 및 관절염(Patey O, et al., J Clin Microbiol. 1997;35:441-445.)을 일으키고;Corynebacterium diphtheria fungi include respiratory diphtheria (Dobie RA, et al., JAMA. 1979; 242: 2197-2201), myocarditis (Boyer NH, et al., N Engl J Med. 1948; 239: 913. ), Eye movement and cilia palsy (Kallick CA, et al., III Med J. 1970; 137: 505-512; and Naiditch MJ, et al., Am J Med. 1954; 17: 229-245), face Dysfunction of the hyperpharyngeal and larynx, peripheral neuritis, cutaneous diphtheria (Koopman JS, et. Al., J Infect Dis. 1975; 131: 239-244), endocarditis (Tiley SM, et al., Clin Infect Dis. 1993; 16: 271-275), fungal aneurysms (Gruner E, et al., Clin Infect Dis. 1994; 18: 94-96), osteomyelitis (Patey O, et al., J Clin Microbiol. 1997; 35: 441-445 ) And arthritis (Patey O, et al., J Clin Microbiol. 1997; 35: 441-445.);

크레브시엘라 옥시토카 균은 폐렴(Korvick JA et al., South. Med. J. 84(2), 200-204, (1991); 및 Al-Moamary MS et al., Clin. Infect. Dis. 26(3), 765-766, (1998)), 소아에서 급성 신우신염(Ghiro L et al., Nephron. 90(1), 8-15, (2002)), 신생아 중환자실에서 돌연사(outbreak)(Jeong SH et al., J. Hosp. Infect, 48(4), 281-288, (2001)), 장염(Soussi F et al., Gastroenterol. Clin. Biol. 25(8-9), 814-816, (2001)을 일으키고;Crevciella oxytoca bacteria include Pneumonia (Korvick JA et al., South. Med. J. 84 (2), 200-204, (1991); and Al-Moamary MS et al., Clin. 26 (3), 765-766, (1998)), acute pyelonephritis in children (Ghiro L et al., Nephron. 90 (1), 8-15, (2002)), outbreak in neonatal intensive care unit (Jeong SH et al., J. Hosp. Infect, 48 (4), 281-288, (2001)), enteritis (Soussi F et al., Gastroenterol. Clin. Biol. 25 (8-9), 814- 816, (2001);

오크로박트룸 안트로피 균은 혈관내 도관과 관련된 균혈증(Kern W.V. et al., Infection, 21, 306-310, (1993)), 심내막염(Mahmood M.S. et al., J. Infect., 40, 287-290, (2000)), 내안구염(Berman A.J. et al., Am. J. Ophthalmol., 123, 560-562, (1997)), 췌장 농양, 괴사성 근막염(Brivet F. et al., Clin. Infect. Dis., 17, 516-518, (1993)), 연골염(Barson W.J. et al., J. Clin. Microbiol., 25, 2014-2016, (1987)) 등을 일으키고;Ocrobact antropy bacteria are known as bacteremia associated with vascular conduits (Kern WV et al., Infection, 21, 306-310, (1993)), endocarditis (Mahmood MS et al., J. Infect., 40, 287 -290, (2000)), endophthalmitis (Berman AJ et al., Am. J. Ophthalmol., 123, 560-562, (1997)), pancreatic abscess, necrotic fasciitis (Brivet F. et al., Clin Infect. Dis., 17, 516-518, (1993)), chondritis (Barson WJ et al., J. Clin. Microbiol., 25, 2014-2016, (1987)), and the like;

펩토스트렙토코코스 프레보티 균은 인체 내에서 여러 가지 농양(예, 뇌농양), 만성 중이염, 급성 유양돌기염, 만성 부비동염, 폐렴, 폐농양, 농흉, 여성 생식기 감염증(Murdoch DA et al., J. Med. Microbiol. 41, 36-44, (1987)), 균혈증(Brook I, J. Infect. Dis. 160, 1071-1075, (1989)), 골수염, 척추 골수염, 유방염, 봉와직염, 괴사성 근막염(Murdoch DA, Clin. Michrobiol. Rev., 11, 81-120, (1998)), 당뇨병성 족부 감염(Wren MWD, Br. J. Biomed. Sci., 53, 294-301, (1996)), 산후 패혈증과 인공관절과 관련된 세균성 관절염(Brook I et al., Am. J. Med. 94, 21-28, (1993)), 심내막염, 심장 판막 주위 농양, 세균성 심막염, 종격염(Murdoch DA, Clin. Microbiol. Rev., 11, 81-120, (1998), 구강내 감염(Finegold SM, New York: Academic; 1977)을 일으키고;Peptostreptococcus prevoti bacteria can cause various abscesses (eg, brain abscesses), chronic otitis media, acute mastoiditis, chronic sinusitis, pneumonia, lung abscesses, empyema, female genital infections (Murdoch DA et al., J. Med). Microbiol. 41, 36-44, (1987)), Bacteremia (Brook I, J. Infect. Dis. 160, 1071-1075, (1989)), osteomyelitis, spinal osteomyelitis, mastitis, cellulitis, necrotizing fasciitis (Murdoch) DA, Clin. Michrobiol. Rev., 11, 81-120, (1998)), diabetic foot infection (Wren MWD, Br. J. Biomed. Sci., 53, 294-301, (1996)), postpartum sepsis And arthritis related to articular joints (Brook I et al., Am. J. Med. 94, 21-28, (1993)), endocarditis, pericardial abscess, bacterial pericarditis, mediastinitis (Murdoch DA, Clin. Microbiol Rev., 11, 81-120, (1998), causing oral infections (Finegold SM, New York: Academic; 1977);

포피로모나스 진지발리스 균은 구강내 감염, 치주 농양, 치주염, 급성 괴사성 괴양성 치주염(Darby I et al., Periodontol. 2000, 26, 33-53, (2001)), 유방 농양(Edmiston CE et al., J. Infect. Dis., 162, 695-699, (1990)), 만성 골수염(Brook I. et al., Am. J. Med. 94(1), 21-28, (1993)), 인후염(Brook I., J. Fam. Pract., 38(2), 175-179, (1994)), 폐렴, 폐농양, 농흉, 중이염, 창상 감염, 복막염, 조갑주위염, 만성 부비동염(Brook I., J. Med. Microbiol. 42(5), 340-347, (1995)), 질염, 골반내 감염(Buerden BI, FEMS Immunol. Med. Microbiol. 6(2-3), 223-227, (1993)), 균혈증(Lee SC et al., J. Microbiol. Immunol. Infect. 32(3), 213-216, (1999)), 심내막염(van Winkelhoff AJ et al., Periodontol. 2000, 20, 122-135, (1999)) 등을 일으키고;Porphyromonas gingivalis is known as oral infection, periodontal abscess, periodontitis, acute necrotic ulcerative periodontitis (Darby I et al., Periodontol. 2000, 26, 33-53, (2001)), breast abscess (Edmiston CE et al., J. Infect. Dis., 162, 695-699, (1990)), chronic osteomyelitis (Brook I. et al., Am. J. Med. 94 (1), 21-28, (1993) ), Sore throat (Brook I., J. Fam. Pract., 38 (2), 175-179, (1994)), pneumonia, lung abscess, empyema, otitis media, wound infection, peritonitis, peritonitis, chronic sinusitis I., J. Med. Microbiol. 42 (5), 340-347, (1995)), vaginitis, pelvic infection (Buerden BI, FEMS Immunol. Med. Microbiol. 6 (2-3), 223-227, (1993)), bacteremia (Lee SC et al., J. Microbiol. Immunol. Infect. 32 (3), 213-216, (1999)), endocarditis (van Winkelhoff AJ et al., Periodontol. 2000, 20, 122-135, (1999)) and the like;

*펩토스트렙토코커스 언애어로비우스 균은 농양(Murdoch D. A. et al., J Med Microbiol 1994; 41: 36-44; Brook I., J Urol 1989; 141: 889-893; Brook I., Ann Otol Rhin Laryngol 1998; 107: 959-960; 및 Civen R. et al,, J Oral Pathol Med 2000; 29: 507-513), 치질 감염(Brook I. and Frazier E. H., Am J Gastroenterol 1996; 91: 333-335), 연부조직 감염(Brook I. and Frazier E. H., Arch Surg 1990; 125: 1445-1451), 심내막염(Montejo M. et al., Clin Infect Dis 1995; 20: 1431), 치은염과 치주염(Moore LVH, et al., J Dent Res 1987; 66: 989-995; 및 Wade WG, et al., J Clin Periodontol 1992; 19: 127-134), 등을 일으키고;Peptostreptococcus E. aerobius is an abscess (Murdoch DA et al., J Med Microbiol 1994; 41: 36-44; Brook I., J Urol 1989; 141: 889-893; Brook I., Ann Otol Rhin Laryngol 1998; 107: 959-960; and Civen R. et al, J Oral Pathol Med 2000; 29: 507-513), hemorrhoidal infection (Brook I. and Frazier EH, Am J Gastroenterol 1996; 91: 333-335) , Soft tissue infection (Brook I. and Frazier EH, Arch Surg 1990; 125: 1445-1451), endocarditis (Montejo M. et al., Clin Infect Dis 1995; 20: 1431), gingivitis and periodontitis (Moore LVH, et al., J Dent Res 1987; 66: 989-995; and Wade WG, et al., J Clin Periodontol 1992; 19: 127-134), and the like;

펩토스트렙토코커스 마그누스 균은 화농성 비인두염(Brook, I., et al., Arch. Otolaryngol. Head eck Surg. 122:4184, 1996), 농흉(Civen, R., H. et al., Clin. Infect. Dis. 20(Suppl. 2):S224S229, 1995; Marina, M., C. et al., Clin. Infect. Dis. 16(Suppl. 4):S256S262, 1993; 및 Murdoch, D. A., et al., J. Med. Microbiol. 41:3644, 1987), 괴사성 폐렴, 간농양(Brook, I. and E. H. Frazier. Pediatr. Infect. Dis. J. 12:743747, 1993), 몸통 표면 감염(Brook, I. and E. H. Frazier. Arch. Surg. 125:144514, 1990), 당뇨성 족부질환의 감염(Sanderson, P. J., Clin. Pathol. 30:266268, 1977), 급만성 비임신성 유방 농양, 봉와직염(Brook, I., and E. H. Frazier., Arch. Surg. 130:786792, 1995), 심내막염(Cofsky, R. D., and S. J. Seligman. 1985. Peptococcus magnus endocarditis. South. Med. J. 78:361362; 및 Pouedras, P., et al., Clin, Infect. Dis. 15:185), 뇌막염(Brown, M. A., et al., Am. J. Med. Sci. 308:18418, 1994), 골수염(Brook, I., and E. H. Frazier., Am. J. Med. 94:22128, 1993), 패혈증성의 관절염(Fitzgerald, R. H., et al., Clin. Orthoped. 164:14114, 1982), 화농성 심막염(Phelps, R., et al., JAMA 254:9479, 1985), 부비동염, 소아 중이염(Brook, I. 1994. Peptostreptococcal infection in children. Scand. J. Infect. Dis. 26:503510. and Clin. Microbiol. Rev. 8:4794) 등을 일으키고;Peptostreptococcus magnus is known as purulent nasopharyngitis (Brook, I., et al., Arch. Otolaryngol.Head eck Surg. 122: 4184, 1996), empyema (Civen, R., H. et al., Clin.Infect) Dis. 20 (Suppl. 2): S224S229, 1995; Marina, M., C. et al., Clin. Infect. Dis. 16 (Suppl. 4): S256S262, 1993; and Murdoch, DA, et al. , J. Med. Microbiol. 41: 3644, 1987), necrotic pneumonia, liver abscess (Brook, I. and EH Frazier. Pediatr. Infect. Dis. J. 12: 743747, 1993), torso surface infection (Brook, I and EH Frazier.Arch.Surg. 125: 144514, 1990), infections of diabetic foot disease (Sanderson, PJ, Clin. Pathol. 30: 266268, 1977), acute nonpregnant breast abscess, cellulitis (Brook, I , and EH Frazier., Arch. Surg. 130: 786792, 1995), endocarditis (Cofsky, RD, and SJ Seligman. 1985. Peptococcus magnus endocarditis.South.Med. J. 78: 361362; and Pouedras, P., et al., Clin, Infect. Dis. 15: 185), meningitis (Brown, MA, et al., Am. J. Med. Sci. 308: 18418, 1994), osteomyelitis (Brook, I., and EH Frazie) r., Am. J. Med. 94: 22128, 1993), septic arthritis (Fitzgerald, RH, et al., Clin. Orthoped. 164: 14114, 1982), purulent pericarditis (Phelps, R., et al. , JAMA 254: 9479, 1985), Sinusitis, Otitis Media in Children (Brook, I. 1994. Peptostreptococcal infection in children. Scand. J. Infect. Dis. 26: 503510. and Clin. Microbiol. Rev. 8: 4794);

푸소박테리움 네크로포룸 균은 구강, 장관과 질내 감염(Mandell: Principles and Practice of Infectious Diseases, 5th ed., Copyright 2000 Churchill Livingstone, Inc p.2564-2566), Lemierre's 증후군 (Bilateral Lemierre's syndrome: a case report and literature review.Ear Nose Throat J. 2002 Apr;81(4):234-6, 238-40, 242)을 일으키고;Fusobacterium necrophorum is a disease of the oral, intestinal and vaginal infections (Mandell: Principles and Practice of Infectious Diseases, 5th ed., Copyright 2000 Churchill Livingstone, Inc p.2564-2566), Lemierre's syndrome (Bilateral Lemierre's syndrome: a case report and literature review. Ear Nose Throat J. 2002 Apr; 81 (4): 234-6, 238-40, 242);

프로테우스 불가리스 균은 요로 감염(Silverblatt FJ. J Exp Med. 1974;140:1696; 및 Wray SK, Hull SI, Cook RG, et al. Proteus mirabilis. Infect Immun. 1986;54:43-49; 및 Mobley HL, Chippendale GR. J Infect Dis. 1990;161:525-530), 수막염, 해면체 정맥 혈전증(Bodur H, Colpan A, Gozukuck R et al.Scand J Infect Dis. 2002;34(9):694-6)을 일으키고;Proteus vulgaris are known for urinary tract infections (Silverblatt FJ. J Exp Med. 1974; 140: 1696; and Wray SK, Hull SI, Cook RG, et al. Proteus mirabilis. Infect Immun. 1986; 54: 43-49; and Mobley HL , Chippendale GR.J Infect Dis. 1990; 161: 525-530), meningitis, cavernous venous thrombosis (Bodur H, Colpan A, Gozukuck R et al. Scand J Infect Dis. 2002; 34 (9): 694-6) Causing it;

엔테로박터 에어로게네스 균은 심부 감염(De Gheldre Y, Maes N, Rost F, et al. J Clin Microbiol. 1997;35:152-160), 비정형 폐렴(Holden DA, Stoller JK. Department of Pulmonary Disease, Cleveland Clinic Foundation, Ohio, West J Med 1992 Jan;156(1):79-824) 등을 일으키고;Enterobacter aerogenes infections include deep infections (De Gheldre Y, Maes N, Rost F, et al. J Clin Microbiol. 1997; 35: 152-160), atypical pneumonia (Holden DA, Stoller JK. Department of Pulmonary Disease, Cleveland Clinic Foundation, Ohio, West J Med 1992 Jan; 156 (1): 79-824) and the like;

스트렙토코커스 뮤탄스 균은 심내막염(Infective Endocarditis in Adults Eleftherios Mylonakis, M.D., and Stephen B. Calderwood, M.D. In New England Journal of medicine Volume 345:1318-1330 November 1, 2001), 균혈증(Elting LS, Bodey GP, Keefe BH. Clin Infect Dis. 1992;14:1201-1207), 뇌막염(Hoyne AL, Herzon H. Ann Intern Med. 1950;33:879-902), 폐렴(Lorber B, Swenson RM. Ann Intern Med. 1974;81:329-331), 급성 화농성 침샘염(Raad II, Sabbagh MF, Caranasos GJ. Clin Infect Dis. 1990;12:591-601), 구강안면치원성 감염(Gill Y, Scully C. Oral Surg Oral Med Oral Pathol. 1990;70;155-158), 내안구염(Principles and Practice of Infectious Diseases. 5th edition. Handell, Churchill Livingstone p.217), 중이염, 부비동염(Gaudreau C, Delage G. Rousseau D, et a. Can Med Assoc J. 1981;125:1246-1249) 등을 일으키고;Infective Endocarditis in Adults Eleftherios Mylonakis, MD, and Stephen B. Calderwood, MD In New England Journal of medicine Volume 345: 1318-1330 November 1, 2001), and bacteremia (Elting LS, Bodey GP, Keefe BH. Clin Infect Dis. 1992; 14: 1201-1207), meningitis (Hoyne AL, Herzon H. Ann Intern Med. 1950; 33: 879-902), pneumonia (Lorber B, Swenson RM. Ann Intern Med. 1974 81: 329-331), acute purulent salivary glanditis (Raad II, Sabbagh MF, Caranasos GJ. Clin Infect Dis. 1990; 12: 591-601), oral facial infection (Gill Y, Scully C. Oral Surg Oral); Med Oral Pathol. 1990; 70; 155-158), Principles and Practice of Infectious Diseases.5th edition.Handell, Churchill Livingstone p.217, Otitis Media, Sinusitis (Gaudreau C, Delage G. Rousseau D, et a Can Med Assoc J. 1981; 125: 1246-1249) and the like;

킨젤라 킨갭 균은 감염성 관절염, 골수염(Amir J, Schockelford PG, J Clin Microbiol. 1991;29:1083-1086; 및 Woolfrey BF, Lally RT, Faville RJ. Am J Clin Pathol. 1986;85:745-749), 심내막염(Wolff AH, Ullman RF, Strampfer MJ, Cunha BA. Heart Lung. 1987;16:579-583; Rabin RL, Wong P, Noonan JA, Plumley DD. Am J Dis Child. 1983;137:403-404; 및 Verbruggen A-M, Hauglustaine D, Schildermans F, et al. J Infect. 1986;13:133-142), 균혈증(Yagupsky P, Dagan R. Pediatr Infect Dis J. 1994;13:1148-1149; Birgisson H, Steingrimsson O, Gudnason T. Scand J Infect Dis. 1997;29:495-498; Roiz MP, Peralta FG, Arjona R. J Clin Microbiol, 1997;35:1916; Yagupsky P, Dagan R. Clin Infect Dis. 1997;24:860-866; 및 Redfield DC, Overturf GD, Ewing N, Powars D. Arch Dis Child. 1980;55:411-414), 폐렴, 후두덮개염, 뇌막염, 농양, 눈의 감염(Yagupsky P, Dagan R, Howard CW, et al. J Clin Microbiol. 1992;30:1278-1281; Kennedy CA, Rosen H. Am J Med. 1988;85:701-702; 및 Mollee T, Kelly P, Tilse M. J Clin Microbiol. 1992;30:2516-2517) 등을 일으키고;The Kinzela Kinggap fungus has been described as infectious arthritis, osteomyelitis (Amir J, Schockelford PG, J Clin Microbiol. 1991; 29: 1083-1086; and Woolfrey BF, Lally RT, Faville RJ. Am J Clin Pathol. 1986; 85: 745-749 ), Endocarditis (Wolff AH, Ullman RF, Strampfer MJ, Cunha BA.Heart Lung. 1987; 16: 579-583; Rabin RL, Wong P, Noonan JA, Plumley DD.Am J Dis Child.1983; 137: 403- 404; and Verbruggen AM, Hauglustaine D, Schildermans F, et al. J Infect. 1986; 13: 133-142), bacteremia (Yagupsky P, Dagan R. Pediatr Infect Dis J. 1994; 13: 1148-1149; Birgisson H , Steingrimsson O, Gudnason T. Scand J Infect Dis. 1997; 29: 495-498; Roiz MP, Peralta FG, Arjona R. J Clin Microbiol, 1997; 35: 1916; Yagupsky P, Dagan R. Clin Infect Dis. 1997 24: 860-866; and Redfield DC, Overturf GD, Ewing N, Powars D. Arch Dis Child. 1980; 55: 411-414), pneumonia, laryngitis, meningitis, abscess, eye infection (Yagupsky P, Dagan R, Howard CW, et al. J Clin Microbiol. 1992; 30: 1278-1281; Kennedy CA, Rosen H. Am J Med. 1988; 85: 701-702; and Mollee T, Kelly P, Ti l M. J Clin Microbiol. 1992; 30: 2516-2517) and the like;

박테로이데스 오바투스 균은 뇌막염, 뇌농양, 인두염, 이하선염, 복부내 감염, 설사, 여성 생식기 감염, 골수염, 패혈증성 관절염(Handell 5th , chapter 237 Bacteroides, Prevotella. Porphyromonas, and Fusobacterium Species and Other Medically Important Anaerobic Gram-Negative Bacilli, p, 2561- 2570)등을 일으키고;Bacterides obatus bacteria are used for meningitis, cerebral abscess, pharyngitis, mumps, abdominal infection, diarrhea, female genital infections, osteomyelitis, and septic arthritis (Handell 5th, chapter 237 Bacteroides, Prevotella. Porphyromonas, and Fusobacterium Species and Other Medically Important Anaerobic Gram-Negative Bacilli, p, 2561- 2570), and the like;

박테로이데스 테타이오타오미크론 균은 항생제와 연관된 장염(George WL, Rolfe RD, Finegold SM. J Clin Microbiol. 1982;15:1049-1053; 및 Smith JA, Cooke DL, Hyde S, et al. J Med Microbiol. 1997;46:953-958)을 일으키고;Bacteroides tetaotaomicron bacteria have been described as enteritis associated with antibiotics (George WL, Rolfe RD, Finegold SM. J Clin Microbiol. 1982; 15: 1049-1053; and Smith JA, Cooke DL, Hyde S, et al. Med Microbiol. 1997; 46: 953-958);

해모필러스 아프로필라스 균은 국소적으로 머리나 호흡기 감염, 부비동염, 중이염, 폐렴(Kiddy K, Webberley J. J Infect. 1987;15:161-163), 농흉, 균혈증, 심내막염(Geraci JE, Wilkowske CJ, Wilson WR, et al. Mayo Clin Proc. 1977;52:209-215), 감염성 관절염, 골수염(Petty BG, Burrow CR,Robinson RA, et al. Am J Med. 1985;78:159-162), 연조직 농양, 창상 감염, 괴사성 근막염, 뇌막염(Petty BG, Burrow CR, Robinson RA, et al, Am J Med. 1985;78:159-162), 뇌 농양(Kilian M., J Gen Microbiol. 1976;93:9-62; Page MI, King EO. Engl J Med. 1966;275:181-188; Sutter VL, Finegold SM. Ann NY Acad Sci. 1970;174:468-487; Kraut MS, Attebery HR, Finegold SM, et al. J Infect Dis. 1972;126:189-192; Elster SK, Mattes LM, Meyers BR, et al. Am J Cardiol. 1975;35:72-79; 및 Bieger RC, Brewer NS, Washington JA II, Medicine(Baltimore). 1978;57:345-355)을 일으키고;Haemophilus apropylas can be used locally for head or respiratory infections, sinusitis, otitis media, pneumonia (Kiddy K, Webberley J. J Infect. 1987; 15: 161-163), empyema, bacteremia, endocarditis (Geraci JE, Wilkowske) CJ, Wilson WR, et al. Mayo Clin Proc. 1977; 52: 209-215), infectious arthritis, osteomyelitis (Petty BG, Burrow CR, Robinson RA, et al. Am J Med. 1985; 78: 159-162) , Soft tissue abscess, wound infection, necrotizing fasciitis, meningitis (Petty BG, Burrow CR, Robinson RA, et al, Am J Med. 1985; 78: 159-162), brain abscess (Kilian M., J Gen Microbiol. 1976 ; 93: 9-62; Page MI, King EO. Engl J Med. 1966; 275: 181-188; Sutter VL, Finegold SM. Ann NY Acad Sci. 1970; 174: 468-487; Kraut MS, Attebery HR, Finegold SM, et al. J Infect Dis. 1972; 126: 189-192; Elster SK, Mattes LM, Meyers BR, et al. Am J Cardiol. 1975; 35: 72-79; and Bieger RC, Brewer NS, Washington JA II, Medicine (Baltimore) 1978; 57: 345-355);

나이세리아 고노헤아 균은 남성의 생식계에서 급성 요도염, 급성 부고환염, 생식기 주변 림프염, 요도주위 농양, 급성 전립선염, 타이슨샘과 카우퍼샘의 감염질환(Cohen MS, Cannon JG, Jerse AE, et al. J Infect Dis. 1994;169:532-537), 여성의 생식계에서 자궁경부염과 요도염, 수란관염(Platt R, Rice PA, McCormack WM. JAMA. 1983;250:3205-3209), 동성애자에서 항문직장 임질, 인두임질(Handsfield HH, Knapp JS, Diehr PK, et al. Sex Transm Dis. 1980;7:1-5), 안구감염, 급성 구개염, 구강 궤양, 피부 감염, 구강내 농양, 골반 염증성 질환(Quinn TC, Stamm WE, Goodell SE, et al. N Engl J Med. 1983;309:576-582) 등을 일으키고;Neisseria gonohea bacteria have acute urethritis, acute epididymitis, perigenital lymphadenitis, peri-urinary abscess, acute prostatitis, infectious diseases of Tyson's gland and cow's gland (Cohen MS, Cannon JG, Jerse AE, et al. J Infect Dis. 1994; 169: 532-537), cervicitis and urethritis in women's reproductive system, plaqueitis (Platt R, Rice PA, McCormack WM. JAMA. 1983; 250: 3205-3209), anal rectum in homosexuals Gonorrhea, pharyngeal gonorrhea (Handsfield HH, Knapp JS, Diehr PK, et al. Sex Transm Dis. 1980; 7: 1-5), eye infections, acute palatitis, oral ulcers, skin infections, oral abscesses, pelvic inflammatory diseases (Quinn TC, Stamm WE, Goodell SE, et al. N Engl J Med. 1983; 309: 576-582) and the like;

아이케넬라 코로덴스 균은 사람이 무는 것(Goldstein EJC. Clin Infect Dis. 1992;14:633-640), 두경부 감염(Tveteras K, Kristensten S, Bach V, et al. J Laryngol Otol. 1987;101:592-594), 호흡기 감염(Suwanagool S, Rothkopf MM, Smith SM, et al. Arch Intern Med. 1983;143:2265-2268), 부인과적 감염(Jeppson KG, Reimer LG. Obstet Gynecol. 1991;78:503-505; Drouet E, De Montclos H, Boude M, et al. Lancet. 1987;2:1089), 폐감염(Joshi N, O'BryanT, Appelbaum PC. Rev Infect Dis. 1991;13:1207-1212), 심내막염(Decker MD, Graham BS, Hunter EB, et al. Am J Med Sci. 1986;292:209-212) 등을 일으키고;E.ella corodens is a human bite (Goldstein EJC. Clin Infect Dis. 1992; 14: 633-640), head and neck infections (Tveteras K, Kristensten S, Bach V, et al. J Laryngol Otol. 1987; 101; : 592-594), respiratory infections (Suwanagool S, Rothkopf MM, Smith SM, et al. Arch Intern Med. 1983; 143: 2265-2268), gynecological infections (Jeppson KG, Reimer LG. Obstet Gynecol. 1991; 78 503-505; Drouet E, De Montclos H, Boude M, et al. Lancet. 1987; 2: 1089), lung infection (Joshi N, O'Bryan T, Appelbaum PC. Rev Infect Dis. 1991; 13: 1207- 1212), causing endocarditis (Decker MD, Graham BS, Hunter EB, et al. Am J Med Sci. 1986; 292: 209-212), and the like;

박테로이데스 불가투스 균은 뇌막염, 뇌농양, 인두염, 이하선염, 복부내 감염, 설사, 여성 생식기 감염, 골수염, 패혈증성 관절염(Mandell 5th, chapter 237 Bacteroides, Prevotella, Porphyromonas, and Fusobacterium Species and Other Medically Important Anaerobic Gram-Negative Bacilli, p. 2561- 2570)을 일으키고;Bacterides vulgaris is common in meningitis, brain abscess, pharyngitis, mumps, abdominal infection, diarrhea, female genital infection, osteomyelitis, septic arthritis (Mandell 5th, chapter 237 Bacteroides, Prevotella, Porphyromonas, and Fusobacterium Species and Other Medically Important Anaerobic) Gram-Negative Bacilli, p. 2561- 2570);

브란하멜라 카타르할리스 균은 중이염(Stenfors L-E, Raisanen S. J Laryngol Otol. 1990;104:749-757), 하부기도 감염, 만성 폐쇄성 호흡기 질환자의 급성 악화(Verghese A, Roberson D, Kalbfleisch JH, Sarubbi F. Antimicrob Agents Chemother. 1990;34:1041-1044), 폐렴(Collazos J, de Miguel J, Ayarza R. Eur J Clin Microbiol Infect Dis. 1992;11:237-240), 호흡기 감염(McKenzie H, Morgan MG, Jordens JZ, et al. J Med Microbiol, 1992;37:70-76), 부비동염(Pentilla M, Savolainen S, Kuikaanniemi H, et al. Acta Otolaryngol (Stockh). 1997;(Suppl) 529:S165-S168), 균혈증(Ioannidis JPA, Worthington M, Griffiths JK, Snydman DR. Clin Infect Dis. 1995;21:390-397)을 일으키고,Branhamela catarrhalis fungus is known as otitis media (Stenfors LE, Raisanen S. J Laryngol Otol. 1990; 104: 749-757), lower respiratory tract infections, and acute exacerbations of chronic obstructive respiratory disease (Verghese A, Roberson D, Kalbfleisch JH, Sarubbi F. Antimicrob Agents Chemother. 1990; 34: 1041-1044), pneumonia (Collazos J, de Miguel J, Ayarza R. Eur J Clin Microbiol Infect Dis. 1992; 11: 237-240), respiratory infections (McKenzie H, Morgan MG, Jordens JZ, et al.J Med Microbiol, 1992; 37: 70-76), Sinusitis (Pentilla M, Savolainen S, Kuikaanniemi H, et al. Acta Otolaryngol (Stockh). 1997; (Suppl) 529: S165- S168), causing bacteremia (Ioannidis JPA, Worthington M, Griffiths JK, Snydman DR. Clin Infect Dis. 1995; 21: 390-397),

수테렐라 와즈워텐시스 균은 충수돌기염, 복막염, 복강내 농양(Clin Infect Dis. 1997;25(Suppl 2):S88-S93)을 일으키고;Suterella wazworthissis causes appendicitis, peritonitis, intraperitoneal abscess (Clin Infect Dis. 1997; 25 (Suppl 2): S88-S93);

캔디다 알비칸스 균은 아구창(Schultz, F.W 1925. Am.J.Dis.Child, 29;283-285), 설염(Bassiouny, A et al. 1984. J.Laryngol.Otol.,98; 609-611), 구내염(Olsen, et al. 1978. Scand.J.Dent.Res, 86;392-398), 질염(Ryley,J.F, J.Med.Vet.Mycol., 24;5-22, 1986), 기관지 폐렴(Plummers,N.S. 1966 Symposium on Candida Infection. London, Churchill Livingstone,pp214-220), 식도염, 위염, 장염(Trier,J.S 1984. Am.J.Med.,77;39-43), 만성적 점막캔디다증(Jorizzo,J.L.1982. Arch. Dermatol., 118; 963-965), 조갑 진균증(Ray, T.L et al.1978. Int.J.Dermatol.,17;603-690), 기저귀 관련 질환(Leyden,J.J 1978. Arch. Dermatol.,114;56-59), 캔디다 육아종(Imperator,P.J 1968. Arch. Dermatol., 97;139-146), 심내막염(Ben Joseph, 1985. Harefuah,108;72-73), 비뇨기계 감염(Goldberg,P.K 1979. J.Am.Med.Assoc.,241;582-584), 수막염(Roessman.1967. Arch.Pathol.,84;495-498), 패혈증(Ashcraft,K 1970. J.Am.Med.Assoc.,217;454-456), 습진(Drouet, M. 1985. Allergie Immunol., 17;13-18), 천식(Wengrower,D et al. 1985. Respiration, 47; 209-213) 등을 일으키고;Candida albicans bacteria include thrush (Schultz, FW 1925. Am. J. Dis. Child, 29; 283-285), Sulsy (Bassiouny, A et al. 1984. J. Laryngol. Otol., 98; 609-611) Stomatitis (Olsen, et al. 1978. Scand. J. Dent. Res, 86; 392-398), vaginitis (Ryley, JF, J. Med. Vet. Mycol., 24; 5-22, 1986), bronchial Pneumonia (Plummers, NS 1966 Symposium on Candida Infection.London, Churchill Livingstone, pp214-220), esophagitis, gastritis, enteritis (Trier, JS 1984. Am. J. Med., 77; 39-43), chronic mucosal candidiasis Jorizzo, JL 1982. Arch. Dermatol., 118; 963-965), Acute mycosis (Ray, TL et al. 1978. Int. J. Dermatol., 17; 603-690), Diaper-related diseases (Leyden, JJ 1978) Arch.Dermatol., 114; 56-59), Candida granulomas (Imperator, PJ 1968. Arch. Dermatol., 97; 139-146), endocarditis (Ben Joseph, 1985. Harefuah, 108; 72-73), urinary Mechanical infection (Goldberg, PK 1979. J. Am. Med. Assoc., 241; 582-584), meningitis (Roessman. 1967. Arch. Pathol., 84; 495-498), sepsis (Ashcraft, K 1970. J Am. Med. Assoc., 217; 454-456, eczema (Drouet, M. 1985. Allergie Immunol., 17; 13). -18), causing asthma (Wengrower, D et al. 1985. Respiration, 47; 209-213) and the like;

캔디다 글라브라타 균은 진균증(Block, C. S., Young, C. N. and Myers, R. A. M. 1977. S. Afr. Med. J 51, 632∼636), 궤사성 화농성 염증, 육아종성 반응(Francis W. Chandler, Williams Kaplan et al. A Colour Atlas and Textbook of the Histopathology of Mycotic Disease. Wolfe Medical Publications Ltd. pp45), 패혈증(Minkowitz, S., D. Koffler, et al. 1963. Am. J. Med., 34:252∼255), 신우신염(Newman, D. M., and J. M. Hogg, et al. 1969. J. Urol., 102:547∼548), 호흡기 감염(Oldfiekld, F. S. J., L. Kapica, et al. 1968. Can. Med. Assoc. J., 98:165∼168), 심내막염(Carmody, T. J., K. K. Kane. 1986. Heart Lung, 15:40∼42; Heffner, D.K., and W. A. Franklin. 1978. Am. J. Clin. Pathol., 70:420∼423; Lees, A. W., S. S. Rau, et al. 1971. Lancet, 1:943∼944), 뇌수막염(Wickerham, L. J. 1957. J. Am. Med. Assoc., 165:47∼48), 안구내염(Larson, P. A., R. L. Lindhstrum, et al. 1978. Arch. Ophthalmol., 96:1019∼1022)을 일으킨다.Candida glabrata is a fungal disease (Block, CS, Young, CN and Myers, RAM 1977. S. Afr. Med. J 51, 632-636), ulcerative purulent inflammation, granulomatous response (Francis W. Chandler, Williams Kaplan et al. A Color Atlas and Textbook of the Histopathology of Mycotic Disease.Wolf Medical Publications Ltd.pp45), sepsis (Minkowitz, S., D. Koffler, et al. 1963. Am. J. Med., 34: 252 255), pyelonephritis (Newman, DM, and JM Hogg, et al. 1969. J. Urol., 102: 547-548), respiratory infections (Oldfiekld, FSJ, L. Kapica, et al. 1968. Can. Med.Assoc. J., 98: 165-168), endocarditis (Carmody, TJ, KK Kane. 1986. Heart Lung, 15: 40-42; Heffner, DK, and WA Franklin. 1978. Am. J. Clin. Pathol., 70: 420-423; Lees, AW, SS Rau, et al. 1971. Lancet, 1: 943-944), meningitis (Wickerham, LJ 1957. J. Am. Med. Assoc., 165: 47- 48), causing ophthalmitis (Larson, PA, RL Lindhstrum, et al. 1978. Arch. Ophthalmol., 96: 1019-1022).

이하, 본 발명을 하기 실시예로 좀더 구체적으로 설명한다. 그러나, 이들 실시예는 단지 예시하는 것으로 본 발명의 범위를 한정하는 것으로 이해되서는 안될 것이다.Hereinafter, the present invention will be described in more detail with reference to the following examples. However, these examples are merely illustrative and should not be understood as limiting the scope of the invention.

(실시예)(Example)

실시예 1Example 1

균의 23S rDNA 및 ITS 염기 서열 결정23S rDNA and ITS sequence determination of bacteria

하기의 균주를 표 1에 기술된 바와 같이 직접 구입하여 각 표준균주의 23S rDNA 및 ITS의 전체 염기 서열을 결정하였다.The following strains were purchased directly as described in Table 1 to determine the total base sequence of 23S rDNA and ITS of each standard strain.

균주를 배양하고 GIAmp DNA mini kit (QIAGEN, USA)을 이용하여 염색체 DNA를 추출하였다. 염기서열을 결정하기 위하여 추출한 DNA를 주형으로 하여 알려져 있는 각 균의 16S, ITS, 23S rDNA 부위를 다중 정렬 (multiple alignment)과 BLAST를 실시하여 상기 예시된 것중 공지된 모든 균에 공통적으로 가지고 있는 공통 프라이머를 제작하였다. 제작된 공통 프라이머 중 ITS부위를 증폭할 수 있는 16S 부위의 공통 프라이머 (1585Fw; 5-TTGTACACACCGCCCGTC)(서열번호 406)와 23S 부위의 공통 프라이머 중 520R, 23S 750F(T), 23S 750F, 970F, 930R, 2960R(T) 및 2960RC는 본 발명자가 직접 제작하였고 나머지 프라이머는 알려져 있는 23S rDNA의 공통 프라이머 (Anthony. R. M., et al., J. Clin. Microbiol. 38(2), 781-788, 2000)를 제작하여 사용하였다. 염기서열 분석시 사용된 제작된 공통 프라이머의 서열 및 그들의 대략적인 위치는 아래 표 2와 같다.Strains were cultured and chromosomal DNA was extracted using a GIAmp DNA mini kit (QIAGEN, USA). Using the extracted DNA as a template to determine the nucleotide sequence, multiple alignment and BLAST of 16S, ITS, and 23S rDNA sites of each known bacterium were carried out to carry out common common to all known bacteria among the above-mentioned examples. Primer was prepared. Among the prepared common primers, a common primer (1585Fw; 5-TTGTACACACCGCCCGTC) (SEQ ID NO: 406) capable of amplifying the ITS site and 520R, 23S 750F (T), 23S 750F, 970F, and 930R among the common primers of the 23S site , 2960R (T) and 2960RC were prepared by the inventors themselves and the remaining primers were known as the common primers of 23S rDNA (Anthony. RM, et al., J. Clin. Microbiol. 38 (2), 781-788, 2000) Was used to produce. Sequences of the prepared common primers used in sequencing and their approximate positions are shown in Table 2 below.

염기서열을 결정하기 위하여 이들 공통 프라이머를 사용하여 균주의 염색체 DNA를 주형으로 하고 PCR을 수행하여 산물을 정제하였다. ITS부분과 23S앞부분의 염기서열을 결정하기 위해서, 알려져 있는 균의 16S rDNA를 가지고 다중 정렬(multiple alignment)과 BLAST를 실시하여 상기 예시된 것중 공지된 모든 균에 공통적으로 가지고 있는 16S rDNA 염기서열 부분을 선정하여 1585Fw 프라이머를 제작하였고, 23BR의 프라이머와 함께 PCR을 수행하였다. PCR 반응은 다음과 같은 조건으로 수행하였다. 첫 번째 변성은 94℃에서 7분간, 두 번째 변성은 94℃에서 1분간 수행하였다. 교잡은 52℃에서 1분, 연장은 72℃에서 1분간 수행하였으며, 이를 10회 반복하였다. 그 후, 다시 세 번째 변성은 94℃에서 1분, 교잡은 54℃에서 1분, 연장은 72℃에서 1분간 수행하였으며, 이를 20회 반복하였다. 이후 72℃에서 5분간 마지막 연장을 1회 수행하였다. PCR 반응결과로 생성된 산물은 아가로스 겔 전기영동법으로 확인하였다. 증폭된 PCR 산물을 정제하고 DNA 자동 스퀀서(Perkin Elmer, ABI prism 3700 sequencer)로 분석하고자 하는 염기서열의 부위에 따라 적절한 프라이머를 사용하여 염기 서열을 결정하였다. 염기서열의 분석시 사용된 프라이머는 일차적으로 제작된 공통 프라이머를 적용하였고 다음으로는 얻어진 염기서열을 기초로 520R, 23S 750F, 23S 750F(T) 프라이머를 제작하여 염기서열을 분석하였다. 상기의 23S rDNA 염기서열의 다음부분을 결정하기 위해서 23BFw와 MS38R을 가지고 PCR을 수행하였다.In order to determine the nucleotide sequence, these common primers were used as a template for chromosomal DNA of the strain, and PCR was performed to purify the product. In order to determine the base sequence of the ITS region and the 23S portion, the 16S rDNA sequence portion which is common to all the known strains by performing multiple alignment and BLAST with 16S rDNA of known bacteria 1585Fw primer was prepared by PCR, and PCR was performed with the primer of 23BR. PCR reaction was performed under the following conditions. The first denaturation was performed at 94 ° C. for 7 minutes and the second denaturation at 94 ° C. for 1 minute. Hybridization was performed at 52 ° C. for 1 minute and extension at 72 ° C. for 1 minute, which was repeated 10 times. Thereafter, the third denaturation was performed at 94 ° C. for 1 minute, hybridization at 54 ° C. for 1 minute, and extension at 72 ° C. for 1 minute. Thereafter, the last extension was performed once at 72 ° C. for 5 minutes. The resulting product was confirmed by agarose gel electrophoresis. The amplified PCR product was purified and sequenced using appropriate primers according to the site of nucleotide sequence to be analyzed by DNA automatic sequencer (Perkin Elmer, ABI prism 3700 sequencer). The primer used in the analysis of the nucleotide sequence was applied to the first prepared common primer, and then the base sequence was analyzed by preparing the 520R, 23S 750F, 23S 750F (T) primer based on the obtained base sequence. PCR was performed with 23BFw and MS38R to determine the next portion of the 23S rDNA sequence.

PCR조건은 상기의 조건과 동일하게 수행하였고, 이것으로 얻어진 염기서열을 바탕으로 970F와 930R의 프라이머를 가지고, 염기서열을 분석하였다. 23S rDNA의 나머지 부분의 염기서열을 결정하기 위해서 기존의 알려져 있는 균들의 23S rDNA의 염기서열을 가지고 다중 정렬 (multiple alignment)과 BLAST를 실시하여 상기 예시된 것중 공지된 모든 균에 공통적으로 가지고 있는 2960R(T), 2960RC 프라이머를 제작하였다. 이 프라이머와 MS37F를 가지고 상기와 동일 조건의 PCR을 수행하였고, 이 프라이머를 이용하여 염기서열을 결정하였다. 이에 따라 결정된 각 균의 23S rDNA 및 ITS의 전체 염기 서열은 서열번호 1 내지 28로 기재되어 있다.PCR conditions were performed in the same manner as above, and based on the base sequence obtained therefrom, primers of 970F and 930R were analyzed, and the base sequence was analyzed. In order to determine the nucleotide sequence of the rest of the 23S rDNA, multiple alignment and BLAST with 23S rDNA nucleotide sequence of known bacteria were performed to carry out 2960R which is common to all known bacteria among those exemplified above. (T), 2960RC primers were prepared. PCR with the same conditions as above was carried out with this primer and MS37F, and the base sequence was determined using this primer. The entire nucleotide sequence of 23S rDNA and ITS of each bacterium thus determined is set forth in SEQ ID NOs: 1 to 28.

실시예 2Example 2

균을 동정하기 위한 DNA 탐침 후보군의 선별Selection of DNA Probe Candidates to Identify Bacteria

각 균의 존재 여부 및 동정을 확인하기 위해서 각 균에만 특이적인 탐침을 제작하였다. 특이 탐침의 제작을 위해서는 각 균에만 특이성을 가져야 하는데 이를 위하여 먼저 그 균에 대한 특이 탐침 후보군을 선정하였다. 특이 탐침 후보군은 상기 실시예 1에서 동정되었거나 GenBank에 공지된 각 균의 23S rRNA 및 ITS 염기 서열과 함께 지금까지 밝혀진 가능한 모든 세균들의 염기서열들을 다중 정렬을 통해서 일렬로 정렬 비교하여 각 균에만 존재하는 특이적인 염기서열을 별도로 구분하여 그 내부에 존재하는 탐침 후보군을 제작하였다. 탐침 후보군은 세균의 경우 23S rRNA 유전자 및/또는 ITS 부위, 진균의 경우 18S rRNA 유전자 부위 내에서 결정하였다. 탐침 후보군의 특이성 여부는 BLAST 검색을 통해 균별간 유사성을 비교하여 확인하였다. 결과로서, 선별된 탐침 후보군은 하기 표 3에 수록되어 있다.In order to confirm the presence and identification of each bacterium, a probe specific to each bacterium was produced. For the production of specific probes, specificity must be unique to each bacterium. For this purpose, a specific probe candidate group for the bacterium was selected first. Specific probe candidate group is present only in each microorganism by aligning and comparing the sequences of all possible bacteria identified in Example 1 or known in GenBank with the 23S rRNA and ITS nucleotide sequences so far through multiple alignments. Specific base sequences were separately classified to prepare probe candidate groups present therein. Probe candidates were determined within the 23S rRNA gene and / or ITS site for bacteria and 18S rRNA gene site for fungi. The specificity of the probe candidate group was confirmed by comparing the similarity between the fungus by BLAST search. As a result, selected probe candidate groups are listed in Table 3 below.

실시예 3Example 3

핵산 탐침의 합성Synthesis of Nucleic Acid Probes

DNA 칩에 적용하기 위해 상기 실시예 2에서 선별된 각 탐침 후보군을 합성하였다. 모노뉴클레오타이드(Proligo Biochemie GmbH Hamburg Co.)를 자동화 합성기(ExpediteTM 8900, PE Biosystems Co.)에 투입하고 목적하는 탐침의 염기서열과 스케일을 입력하여 각각 0.05 umole의 순수한 핵산 탐침을 수득하였다. 수득된 탐침은 전기영동을 하여 합성 여부를 확인하였다.Each probe candidate group selected in Example 2 was synthesized for application to a DNA chip. Mononucleotide (Proligo Biochemie GmbH Hamburg Co.) was put into an automated synthesizer (Expedite ™ 8900, PE Biosystems Co.) and input the sequence and scale of the desired probe to obtain a pure nucleic acid probe of 0.05 umole, respectively. The obtained probe was subjected to electrophoresis to confirm the synthesis.

실시예 4Example 4

DNA 칩의 제작Fabrication of DNA Chips

DNA 탐침을 고정화하기 위해서는 알데히드기-아민기 사이의 결합을 이용하였다. DNA 탐침을 고정화하기 위해 모든 DNA 단편 탐침을 합성할 때, 3-첫번째 위치에 아미노링커컬럼(Aminolinker column, Cruachem, Glasgrow, Scotland)을 이용하여 아민기(amino residue)를 가진 염기를 삽입하였고, 유리판(slide glass)은 알데히드기(aldehyde residue)로 코팅화되어 있는 것을 구입(CEL Associates, Inc. Huston, Texas, USA)하였다.In order to immobilize the DNA probe, a bond between the aldehyde group and the amine group was used. When synthesizing all DNA fragment probes to immobilize the DNA probe, a base having an amino residue was inserted using an aminolinker column (Aminolinker column, Cruachem, Glasgrow, Scotland) in the 3-first position, and a glass plate. Slide glass was coated with an aldehyde residue (CEL Associates, Inc. Huston, Texas, USA).

탐침을 고정시킬때는 탐침을 3X SSC (0.45 M NaCl, 15 mM C6H5Na3O7, pH 7.0) 완충 용액에 용해시킨 상태로 공지 기술에 따라 제작한 마이크로어레이어(microarrayer)를 이용 (Yoon. S. H., et al, J. Microbiol. Biotechnol. 10(1), 21-26, 2000-이 문헌의 전체 내용은 본원에 참고로 원용된다)하여 스폿팅한 후, 55% 정도의 습도가 유지되는 조건에서 1시간 이상 화학 반응을 유도하고 6시간 이상 방치함으로써 DNA 탐침을 고정화하였다. 균 검색용 탐침은 100 pmole의 농도로 전체 탐침을 280 ㎛ 간격을 두고 순서대로 집적화시켜 탐침이 고정된 DNA 칩을 제작하였다.To fix the probe, use a microarrayer made according to a known technique in a state in which the probe was dissolved in 3X SSC (0.45 M NaCl, 15 mM C 6 H 5 Na 3 O 7 , pH 7.0) buffer solution. Yoon, SH, et al, J. Microbiol.Biotechnol. 10 (1), 21-26, 2000—the entire contents of this document are incorporated herein by reference), followed by a humidity of about 55%. The DNA probe was immobilized by inducing a chemical reaction for at least 1 hour and leaving it for at least 6 hours under the above conditions. In the bacteria detection probe, the total probe was integrated at a concentration of 100 pmole at intervals of 280 μm in order to prepare a DNA chip fixed with the probe.

탐침의 아민기와 유리판 위의 알데히드 사이의 반응이 원활히 이루어져 고정화가 잘 이루어졌는지 확인하기 위해 사이브로 그린 II (SYBRO green II, Molecular Probe, Inc., Leiden, Netherlands)로 염색하여 확인하였다.In order to confirm that the reaction between the amine group of the probe and the aldehyde on the glass plate was smooth and well immobilized, it was confirmed by staining with SYBRO green II (Molecular Probe, Inc., Leiden, Netherlands).

실시예 5Example 5

표적 시료의 분리 및 증폭Isolation and Amplification of Target Samples

실시예 1에 기술된 28종의 표준균주를 포함하여 하기 표 3에 수록된 31종의 표준균주로부터 게놈 DNA를 별도로 추출하였다:Genomic DNA was separately extracted from the 31 standard strains listed in Table 3, including the 28 standard strains described in Example 1:

적정 배지에 키운 균을 200㎕ 멸균된 증류수에 현탁하여 14,000rpm에서 10분간 원심분리한 뒤 상층액을 버리고 펠렛만 남겼다.The bacteria grown in the titration medium were suspended in 200 µl sterile distilled water, centrifuged at 14,000 rpm for 10 minutes, and the supernatant was discarded, leaving only pellets.

그람 음성균인 경우, ATL용액(Tissue Lysis용액, DNeasy Tissue Kit, QIAGEN) 180㎕에 부유시키고, Proteinase K 20㎕를 넣어 용균한 후 55℃ 에서 1시간 배양하였다. 15초간 와동(vortexing)을 한 후 AL용액 (Lysis용액, DNeasy Tissue Kit, QIAGEN) 200㎕를 넣어 섞은 후 70℃에서 10분간 배양하였다. 에탄올(100%) 200㎕을 넣고 섞은 후 DNeasy 미니 컬럼이 들어 있는 2-ml 튜브에 전용액을 옮겨 8,000rpm이상에서 1분간 원심분리하여 튜브에 모이는 액을 버렸다. AW1용액(Wash용액1, DNeasy Tissue Kit, Qiagen) 500㎕를 넣고 8,000rpm 이상에서 1분간 원심분리하여 유출물을 버리고 다시 AW2용액(Wash용액2, DNeasy Tissue Kit, QIAGEN) 500㎕를 넣어 전속(full speed)에서 3분간 원심분리하여 DNeasy 막을 말리고, 유출물을 버렸다. DNeasy 미니 컬럼을 넣어 실온에서 15분간 정치한 후 8,000rpm이상에서 1분간 원심분리하여 게놈 DNA를 용출하였다.In case of Gram-negative bacteria, ATL solution (Tissue Lysis solution, DNeasy Tissue Kit, QIAGEN) was suspended in 180 µl, 20 µl of Proteinase K was lysed, and then incubated at 55 ° C for 1 hour. After vortexing for 15 seconds, 200 μl of AL solution (Lysis solution, DNeasy Tissue Kit, QIAGEN) was mixed and incubated at 70 ° C. for 10 minutes. After 200 μl of ethanol (100%) was added and mixed, the solution was transferred to a 2-ml tube containing a DNeasy mini column and centrifuged at 8,000 rpm for 1 minute to discard the liquid collected in the tube. Add 500 µl of AW1 solution (Wash solution 1, DNeasy Tissue Kit, Qiagen), centrifuge at 8,000 rpm or more for 1 minute, discard the effluent, and add 500 µl of AW2 solution (Wash solution 2, DNeasy Tissue Kit, QIAGEN). The DNeasy membranes were dried by centrifugation at full speed for 3 minutes and the effluent was discarded. After inserting the DNeasy mini-column for 15 minutes at room temperature, the genomic DNA was eluted by centrifugation for 1 minute at 8,000 rpm or more.

그람 양성균(표준균주)의 경우, 리소자임 용균 용액(20mm Tris-Cl, pH8.0, 2mM EDTA, 1.2% TritonX-100, 20mg/ml lysozyme) 180㎕에 현탁한 후 37℃에서 30분 이상 배양하여 Proteinase K 25㎕와 AL용액(용균용액, DNeasy Tissue Kit, QIAGEN) 200㎕을 넣어 잘 섞은 뒤 70℃에서 30분간 배양하였다. 에탄올(100%) 200㎕을 넣고 섞은 후 DNeasy 미니 컬럼이 들어 있는 2-ml 튜브에 전용액을 옮겨 8,000rpm 이상에서 1분간 원심분리하여 튜브에 모이는 액을 버렸다. AW1용액(Wash용액1, DNeasy Tissue Kit, Qiagen) 500㎕를 넣고 8,000rpm 이상에서 1분간 원심분리하여 유출물을 버리고 다시 AW2용액(Wash용액2, DNeasy Tissue Kit, QIAGEN) 500㎕를 넣어 전속(full speed)에서 3분간 원심분리하여 DNeasy 막을 말리고, 유출물을 버렸다. DNeasy 미니 컬럼을 넣어 실온에서 15분간 정치한 후 8,000rpm 이상에서 1분간 원심분리하여 게놈 DNA를 용출하였다.In case of Gram-positive bacteria (standard strain), it was suspended in 180 µl of lysozyme lysate solution (20mm Tris-Cl, pH8.0, 2mM EDTA, 1.2% TritonX-100, 20mg / ml lysozyme) and incubated for 30 minutes at 37 ℃. 25 μl of Proteinase K and 200 μl of AL solution (solution solution, DNeasy Tissue Kit, QIAGEN) were mixed well and incubated at 70 ° C. for 30 minutes. After adding 200 μl of ethanol (100%) and mixing, transfer the exclusive solution to a 2-ml tube containing a DNeasy mini column, and centrifuged at 8,000 rpm for 1 minute to discard the liquid collected in the tube. Add 500 µl of AW1 solution (Wash solution 1, DNeasy Tissue Kit, Qiagen), centrifuge at 8,000 rpm or more for 1 minute, discard the effluent, and add 500 µl of AW2 solution (Wash solution 2, DNeasy Tissue Kit, QIAGEN). The DNeasy membranes were dried by centrifugation at full speed for 3 minutes and the effluent was discarded. After inserting the DNeasy mini column for 15 minutes at room temperature, the genomic DNA was eluted by centrifugation for 1 minute at 8,000rpm or more.

진균(표준균주)의 경우는, 200㎕의 멸균된 증류수에 현탁하여 14,000rpm에서 10분간 원심분리한 뒤 상층액을 버리고 펠렛을 수거하였다. 이 펠렛을 200㎕의 SDS TE 완충액(10% SDS, 100mM Tris-Cl, 20mM EDTA, pH 8.0)와 20㎕의 프로테이나제(proteinase) K(DNeasy Tissue kit에 포함) 용액과 혼합하고 55℃에서 2시간 및 95℃에서 10분간 차례로 배양하였다. 배양물에 에탄올(100%) 200㎕을 넣고 섞은 후 이 혼합액을 DNesay 미니 컬럼이 들어 있는 2-㎖ 튜브에 옮겨 8,000rpm 이상에서 1분간 원심분리하여 튜브에 모이는 액을 버렸다. AW1용액(Wash용액1, DNesay Tissue Kit,Cat. No.69504 QIAGEN) 500㎕를 넣고 8,000rpm이상에서 1분간 원심분리하여 유출물을 버리고 다시 AW2용액(Wash용액2, DNesay Tissue Kit, QIAGEN) 500㎕를 넣어 전속(full speed)에서 3분간 원심분리하여 DNeasy 막을 말리고 유출물을 버렸다. DNesay 미니 컬럼을 1.5㎖ 튜브에 옮기고 AE용액(용출액, DNesay Tissue Kit, QIAGEN) 100㎕을 넣어 실온에서 15분간 정치한 후 8,000rpm 이상에서 1분간 원심분리하여 게놈 DNA를 용출시켰다. 다시 한번 더 DNesay 미니 컬럼에 AE용액(용출액, DNesay Tissue Kit, QIAGEN) 100㎕을 넣어 실온에서 15분간 정치한 후 8,000rpm이상에서 1분간 원심분리하여 게놈 DNA를 용출하였다.In the case of fungi (standard strains), the cells were suspended in 200 µl of sterile distilled water, centrifuged at 14,000 rpm for 10 minutes, and the supernatant was discarded and pellets were collected. The pellet is mixed with 200 μl SDS TE buffer (10% SDS, 100 mM Tris-Cl, 20 mM EDTA, pH 8.0) and 20 μl proteinase K (included in the DNeasy Tissue kit) solution at 55 ° C. Incubated for 2 h at and 10 min at 95 ° C. 200 μl of ethanol (100%) was added to the culture, and the mixed solution was transferred to a 2-ml tube containing a DNesay mini column, and centrifuged at 8,000 rpm for 1 minute to discard the liquid collected in the tube. Add 500 µl of AW1 solution (Wash solution 1, DNesay Tissue Kit, Cat.No.69504 QIAGEN), centrifuge at 8,000 rpm or more for 1 minute, discard the distillate, and re-use AW2 solution (Wash solution 2, DNesay Tissue Kit, QIAGEN) 500 Add μl and centrifuge for 3 minutes at full speed to dry the DNeasy membrane and discard the effluent. The DNesay mini column was transferred to a 1.5 ml tube, and 100 µl of AE solution (eluate, DNesay Tissue Kit, QIAGEN) was added thereto, and allowed to stand at room temperature for 15 minutes, followed by centrifugation at 8,000 rpm or more for 1 minute to elute genomic DNA. Once again, 100 μl of an AE solution (eluate, DNesay Tissue Kit, QIAGEN) was added to the DNesay mini column and allowed to stand at room temperature for 15 minutes, followed by centrifugation at 8,000 rpm or more for 1 minute to elute genomic DNA.

상기에서와 같이 분리된 균주의 DNA를 주형으로 하고 비대칭 증폭방법(Asymmetric PCR)을 수행하여 단편 유전자를 제작하였다. 단편 유전자는 전방향 프라이머와 역방향 프라이머의 첨가비율을 1:5로 차별화 함으로써 한번의 중합효소연쇄반응으로 획득하였다. PCR 수행시 탐침과 결합한 DNA를 확인하기 위해 5-FITC(fluorescein isothiocyanate)가 부착된 프라이머를 이용하여 증폭함으로써 형광을 통해 결합을 확인하였다. PCR 반응에 사용한 프라이머는 DNA가 세균으로부터 분리된 경우 다음과 같은 3개 쌍의 프라이머가 동시에 사용되었다:A fragment gene was prepared by using the DNA of the isolated strain as a template and performing asymmetric amplification (Asymmetric PCR). Fragment gene was obtained by one polymerase chain reaction by differentiating the ratio of forward and reverse primers at 1: 5. In order to identify DNA bound to the probe during PCR, binding was confirmed by fluorescence by amplification using a primer attached with 5-FITC (fluorescein isothiocyanate). The primers used in the PCR reaction were used simultaneously with three pairs of primers when DNA was isolated from bacteria:

프라이머 1 (센스): TTGTACACACCGCCCGTC (서열번호 406, 1585Fw)와 Primer 1 (sense): TTGTACACACCGCCCGTC (SEQ ID NOs: 406, 1585Fw)

프라이머 2 (안티센스): F-TTTCGCCTTTCCCTCACGGTACT (서열번호 407, 23BR);Primer 2 (antisense): F-TTTCGCCTTTCCCTCACGGTACT (SEQ ID NOs: 407, 23BR);

프라이머 3 (센스): AGTACCGTGAGGGAAAGGCGAA (서열번호 408, 23BFw)와 Primer 3 (sense): AGTACCGTGAGGGAAAGGCGAA (SEQ ID NOs: 408, 23BFw)

프라이머 4 (안티센스): F-TGCTTCTAAGCCAACATCCT (서열번호 409, 37R); 및Primer 4 (antisense): F-TGCTTCTAAGCCAACATCCT (SEQ ID NOs: 409, 37R); And

프라이머 5 (센스): AGGATGTTGGCTTAGAAGCA (서열번호 410, MS37F)와Primer 5 (Sense): with AGGATGTTGGCTTAGAAGCA (SEQ ID NO: 410, MS37F)

프라이머 6 (안티센스): F-CCCGACAAGGAATTTCGCTACCTT (서열번호 411, MS38R).Primer 6 (antisense): F-CCCGACAAGGAATTTCGCTACCTT (SEQ ID NO: 411, MS38R).

상기 서열의 5' 말단에 부착된 F는 FITC 형광물질을 가리킨다.F attached to the 5 'end of the sequence indicates the FITC fluorescent material.

DNA가 진균으로부터 분리된 경우 다음의 프라이머 쌍이 사용되었다:When DNA was isolated from fungi the following primer pairs were used:

프라이머 1 (센스): GTAATTGGAATGAGTACAAT(서열번호 412, fun463F)와 Primer 1 (sense): GTAATTGGAATGAGTACAAT (SEQ ID NO: 412, fun463F)

프라이머 2 (안티센스): F-CTACGACGGTATCTGATCAT(서열번호 413, fun986R).Primer 2 (antisense): F-CTACGACGGTATCTGATCAT (SEQ ID NO: 413, fun986R).

PCR 반응은 다음과 같은 조건으로 수행하였다. 10X PCR 완충액(100mM Tris-HCl(pH 8.3), 500mM KCl, 15mM MgCl2) 5ul, dNTP 혼합물(dATP, dGTP, dCTP, dTTP 각각 2.5mM) 4ul, 10pmole 전방향 프라이머 0.5ul, 10pmole 역방향 프라이머 2.5ul, 1/10로 희석한 주형 DNA(100ng) 1ul, Taq 폴리미라제(5unit/ul, Takara Shuzo Co., Shiga, Japan) 0.5ul를 첨가후 총 부피가 50ul가 되도록 물을 첨가하였다. 첫 번째 변성은 94℃에서 7분간, 두 번째 변성은 94℃에서 1분간 수행하였다. 교잡(annealing)은 52℃에서 1분, 연장(extension)은 72℃에서 1분간 수행하였으며, 이를 10회 반복하였다. 그 후, 다시 세 번째 변성은 94℃에서 1분, 교잡은 54℃에서 1분, 연장은 72℃에서 1분간 수행하였으며, 이를 30회 반복하였다. 이후 72℃에서 5분간 마지막 연장을 1회 수행하였다. PCR 반응결과로 생성된 산물은 아가로스 겔 전기영동법(agarose gel electrophoresis)으로 확인하였다. 이의 결과, 각 균주들에 대해 DNA 이중 가닥과 단편 가닥이 동시에 합성되었음을 확인하였다.PCR reaction was performed under the following conditions. 5 ul 10X PCR buffer (100 mM Tris-HCl (pH 8.3), 500 mM KCl, 15 mM MgCl 2 ), dNTP mixture (2.5 mM each for dATP, dGTP, dCTP, dTTP) 4 ul, 10 pmole forward primer 0.5 ul, 10 pmole reverse primer 2.5 ul 1 μl of template DNA (100 ng) diluted with 1/10 and 0.5 ul of Taq polymyrase (5 unit / ul, Takara Shuzo Co., Shiga, Japan) were added thereto and then water was added so that the total volume was 50 ul. The first denaturation was performed at 94 ° C. for 7 minutes and the second denaturation at 94 ° C. for 1 minute. Annealing was performed at 52 ° C. for 1 minute and extension at 72 ° C. for 1 minute, which was repeated 10 times. Thereafter, the third denaturation was performed at 94 ° C for 1 minute, hybridization at 54 ° C for 1 minute, and extension at 72 ° C for 1 minute, which was repeated 30 times. Thereafter, the last extension was performed once at 72 ° C. for 5 minutes. The product produced as a result of the PCR reaction was confirmed by agarose gel electrophoresis. As a result, it was confirmed that DNA double strands and fragment strands were simultaneously synthesized for each strain.

실시예 6Example 6

혼성화 및 세척Hybridization and Washing

탐침 후보군의 특이성과 민감도를 확인하기 위하여 균체로부터 증폭된 상기 PCR 생성물(실시예 4)을 핵산 탐침이 고정된 DNA 칩(실시예 3)에 적용하여 혼성화 반응을 실시하였다. 핵산 탐침을 이의 표준균주로부터 분리한 게놈 유전자와 혼성화 반응 후 시그널이 나타나는 경우 나머지 종에 대해서 교차반응(특이성) 여부를 시험하였다.In order to confirm the specificity and sensitivity of the probe candidate group, the hybridization reaction was performed by applying the PCR product (Example 4) amplified from the cells to the DNA chip (Example 3) to which the nucleic acid probe was fixed. The nucleic acid probe was tested for cross-reaction (specificity) for the remaining species when a signal appeared after hybridization with a genomic gene isolated from its standard strain.

유리판에 핵산 탐침이 고정된 DNA 칩에 수증기를 씌워 가수화를 시킨후, 70% 에탄올에 담금질함으로써 유리판에 고정되지 않은 탐침을 제거시켰다. 이후에, 혼성화 과정중에 형광물질이 유리판 표면에 남아있는 알데하이드 잔기에 부착되어 전체적인 시그널을 높여 특이적인 탐침의 시그널을 효과를 저해시키는 것을 방지하기 위해서 유리판을 차단용액(blocking solution)(1.3g NaBH4, 375ml PBS, 125ml 100% 에탄올)에 담근후 진탕기(shaker)에서 5분간 반응시켰다. 0.2% SDS로 5분간 세척후, 멸균수로 1분간 2-3번 세척해준 후, 원심분리기를 이용하여 유리판에 있는 물기를 제거하였다(1,000rpm, 2분간).The DNA plate having the nucleic acid probe immobilized on the glass plate was hydrolyzed by steaming, and then immersed in 70% ethanol to remove the probe that was not immobilized on the glass plate. Subsequently, during the hybridization process, the fluorescent material was attached to an aldehyde residue remaining on the surface of the glass plate, thereby increasing the overall signal and preventing the glass plate from blocking the effect of the specific probe signal (1.3 g NaBH 4). , 375ml PBS, 125ml 100% ethanol) and then reacted for 5 minutes in a shaker (shaker). After washing for 5 minutes with 0.2% SDS, washed 2-3 times with sterile water for 1 minute, and then the water in the glass plate was removed using a centrifuge (1,000rpm, 2 minutes).

혼성화시킬 용액은 혼성화완충용액(hybridization buffer: 6×SSPE; 20XSSPE; 3M NaCl, 0.2M NaH2PO4·H2O, 0.02M EDTA, pH 7.4, 20% (v/v) 포름아미드; Sigma Co., St. Louis)에 비대칭 증폭 방법(Asymmetric PCR)으로 증폭시킨 유전자를 30㎕ 넣어서 총 부피가 200㎕가 되도록 제조하였다. 제조된 용액을 탐침이 고정화되어 있는 유리판 위에 분주한 후 프로브-클립 프레스-씰 배양실(probe-clip press-seal incubation chamber, Sigma Co., St. Louis, MO.)로 덮었다.To hybridization solution hybridization buffer solution (hybridization buffer: 6 × SSPE; 20XSSPE; 3M NaCl, 0.2M NaH 2 PO 4 · H 2 O, 0.02M EDTA, pH 7.4, 20% (v / v) formamide; Sigma Co , St. Louis) was added to the 30 amplified gene amplified by asymmetric amplification method (Asymmetric PCR) was prepared so that the total volume is 200 μl. The prepared solution was dispensed onto the glass plate on which the probe was immobilized and then covered with a probe-clip press-seal incubation chamber (Sigma Co., St. Louis, Mo.).

혼성화되는 동안 유리판 주변이 건조되는 것을 방지하기 위해서 혼성화 챔버에 물기가 있는 휴지를 넣은 후, 30℃ 항온진탕배양기(shaking incubator)에서 6시간 동안 반응시켜 상보적인 결합을 유도하였다. 시간이 경과한 후 3×SSPE (0.45M NaCl, 15mM C6H5Na3O7, pH 7.0), 2×SSPE (0.3M NaCl, 10mM C6H5Na3O7, pH 7.0), 1×SSPE (0.15M NaCl, 5mM C6H5Na3O7, pH 7.0) 순으로 각각 5분씩 세척한 후, 원심분리기를 이용하여 유리판에 있는 물기를 제거하였다(1,000rpm, 2분간).To prevent drying around the glass plate during hybridization, a wet tissue was placed in the hybridization chamber and then reacted for 6 hours in a 30 ° C shaking incubator to induce complementary binding. After elapse of time 3 × SSPE (0.45M NaCl, 15 mM C 6 H 5 Na 3 O 7 , pH 7.0), 2 × SSPE (0.3M NaCl, 10 mM C 6 H 5 Na 3 O 7 , pH 7.0), 1 After washing for 5 minutes in each of SSPE (0.15 M NaCl, 5 mM C 6 H 5 Na 3 O 7 , pH 7.0), the water in the glass plate was removed using a centrifuge (1,000 rpm, 2 minutes).

실시예 7Example 7

하이브리드의 검출Hybrid Detection

혼성화 반응의 결과는 스캔어레이(Scanarray) 5000(GSI Lumonics Inc., Bedford, MA.)을 이용하여 검색하였고, 그 결과는 하기 표 4 내지 표 49에 나타나 있다.The results of the hybridization reaction were searched using Scanarray 5000 (GSI Lumonics Inc., Bedford, MA.), And the results are shown in Tables 4 to 49 below.

표 4 내지 표 49Tables 4 through 49

실시예 8Example 8

블라인드 시험Blind test

실시예 4에서와 같이 DNA 칩을 제작하고 이를 이용하여 12종의 균, 즉 스타괼로코코스 아우레우스, 슈도모나스 애루지노사, 프로테우스 미라빌리스, 크레브시엘라 뉴모니에, 아시네토박터 바우마니, 에스케리치아 콜라이, 엔테로코코스 페슘, 스타필로코코스 에피더미디스, 살모넬라 그룹 E, 살모넬라 그룹 B, 크레브시엘라 옥시토카 및 벌코데리아 세파시아에 대한 블라인드 시험을 실시하였다. 도 1, 9, 11 및 14는 블라인드 시험을 위한 DNA 칩의 디자인을 도시한 것이다. 이들 도면에 기재된 명칭은 상기 표 4 내지 49에 수록된 탐침이 유리판에 고정되어 있는 것을 가리킨다. 포지션 마커로서 아민 3'-AAAAAAAAAAAAAAA-5'-FITC(서열번호, 428)가 사용되었고 C로 표기되어있으며, 네가티브 콘트롤로서 탐침이 녹아있는 버퍼(3X SSC)를 사용하였고 N으로 표기되어있다. 칩상의 포지티브 콘트롤로서 공통의 세균 탐침을 사용하였으며 이는 하기 표 50에 수록되어 있다.A DNA chip was prepared as in Example 4, and 12 kinds of bacteria were used, namely Staphylococcus aureus, Pseudomonas aeruginosa, Proteus mirabilis, Crevciella pneumoniae, and acinetobacter bau. Blind tests on Mani, Escherichia coli, Enterococcus pesium, Staphylococcus epidermidis, Salmonella group E, Salmonella group B, Crevciella oxytoca and Vulcoderia sefacia were conducted. 1, 9, 11 and 14 show the design of DNA chips for blind testing. The names described in these figures indicate that the probes listed in Tables 4 to 49 are fixed to the glass plates. Amine 3'-AAAAAAAAAAAAAAA-5'-FITC (SEQ ID NO: 428) was used as a position marker and is labeled C. As a negative control, a probe-melted buffer (3X SSC) was used and is labeled N. A common bacterial probe was used as a positive control on the chip, which is listed in Table 50 below.

이 시험에 사용된 검체는 약 300명의 병원성 감염 환자의 특정부위로부터 채취된 것으로서, 균의 감염여부는 배양방법에 의해 확인되었다.The samples used in this study were taken from specific sites of about 300 pathogenic infections, and the presence of bacteria was confirmed by the culture method.

배양된 검체로부터 게놈 DNA를 다음과 같이 추출하였다. 검체가 체액인 경우, 10ml의 체액을 EDTA 튜브 혹은 플레인 튜브(plain tube)에 수집하였다. 검체의 양이 10ml 이상이면 5,000 xg에서 15분간 원심분리하고 10ml 이하이면 14,000rpm에서 15분간 원심분리한 뒤 침전물을 1.5ml 튜브 1-2개에 모았다. 리소자임 완충액(20mM Tris-Cl, pH 8.0, 2mM EDTA, 1.2% Triton X-100, 20mg/㎖ lysozyme) 180㎕에 현탁하여 37℃에서 30분간 배양한 후 프로테이나제 K 20㎕와 AL용액 (Lysis용액, QIAamp DNA Blood Mini Kit, QIAGEN) 200㎕를 넣어 부드럽게 섞은 후 55℃에서 2시간 배양하고 다시 95℃에서 10분간 배양하였다. 에탄올 (100%) 200㎕을 넣고 섞은 후 QIAamp 스핀 컬럼이 들어있는 2-㎖ 튜브에 전 용액을 옮겨 8,000rpm에서 1분간 원심분리하여 튜브에 모이는 액을 버렸다. AW1용액(Wash용액1, QIAamp DNA Blood Mini Kit, QIAGEN) 500㎕를 넣고 8,000rpm에서 1분간 원심분리하여 유출물을 버리고 다시 AW2용액(Wash용액2, QIAamp DNA Blood Mini Kit, QIAGEN) 500㎕를 넣어 14,000rpm에서 1분간 원심분리하여 유출물을 버렸다. QIAamp 스핀 컬럼을 1.5㎖ 튜브에 옮기고 AE용액(Elution용액, DNA Blood Mini Kit, QIAGEN) 300㎕을 넣어 실온에서 15분간 정치한 후 8,000rpm에서 3분간 원심분리하여 게놈 DNA를 용출하였다. 에탄올 (100%) 750㎕를 넣어 -20℃에서 1시간 정치한 후 14,000rpm에서 20분간 원심분리한 후 상등물인 에탄올을 버리고 건조시킨 뒤 펠렛은 20㎕ 멸균된 증류수에 녹여 농축시켰다.Genomic DNA was extracted from the cultured samples as follows. If the sample was a bodily fluid, 10 ml of the bodily fluid was collected in an EDTA tube or a plain tube. If the amount of the sample is more than 10ml centrifuged at 5,000 xg for 15 minutes, if less than 10ml was centrifuged for 15 minutes at 14,000rpm and the precipitate was collected in 1-2 1.5ml tube. Suspend in 180µl of lysozyme buffer (20mM Tris-Cl, pH 8.0, 2mM EDTA, 1.2% Triton X-100, 20mg / ml lysozyme) and incubate for 30 minutes at 37 ° C. Lysis solution, QIAamp DNA Blood Mini Kit, QIAGEN) 200µl was gently mixed, incubated for 2 hours at 55 ° C, and then incubated at 95 ° C for 10 minutes. After adding 200 μl of ethanol (100%) and mixing, the whole solution was transferred to a 2-ml tube containing a QIAamp spin column, and centrifuged at 8,000 rpm for 1 minute to discard the liquid collected in the tube. Add 500 µl of AW1 solution (Wash solution 1, QIAamp DNA Blood Mini Kit, QIAGEN), centrifuge at 8,000 rpm for 1 minute, discard the effluent, and then again 500 µl of AW2 solution (Wash solution 2, QIAamp DNA Blood Mini Kit, QIAGEN). The mixture was centrifuged at 14,000 rpm for 1 minute to discard the effluent. The QIAamp spin column was transferred to a 1.5 ml tube, 300 µl of AE solution (Elution Solution, DNA Blood Mini Kit, QIAGEN) was added thereto, and allowed to stand at room temperature for 15 minutes, followed by centrifugation at 8,000 rpm for 3 minutes to elute genomic DNA. After adding 750 μl of ethanol (100%) for 1 hour at −20 ° C., centrifuging at 14,000 rpm for 20 minutes, discarding the ethanol, which was the supernatant, drying, and pellets were dissolved in 20 μl sterile distilled water and concentrated.

검체가 혈액인 경우, 10ml 혈액을 EDTA 튜브에 샘플링하였다. 4℃, 1,800rpm에서 10분간 원심분리하여 혈장 층을 1.5㎖ 튜브에 나누어 담고, 14,000rpm에서 10분간 원심분리 한 뒤 침전물을 1.5㎖ 튜브에 나누었다. 라이소자임 완충액(20mM Tris-Cl, pH 8.0, 2mM EDTA, 1.2% Triton X-100, 20mg/㎖ lysozyme) 180㎕에 현탁하여 37℃에서 30분간 배양한 후 프로테이나제 K 20㎕와 AL용액(Lysis용액, QIAamp DNA Blood Mini Kit, QIAGEN) 200㎕를 넣어 부드럽게 섞은 후 55℃ 에서 30분간 배양하고 다시 95℃에서 10분간 배양하였다. 에탄올(100%) 200㎕을 넣고 섞은 후 QIAamp 스핀 컬럼이 들어 있는 2-㎖ 튜브에 전 용액을 옮겨 8,000rpm에서 1분간 원심분리하여 튜브에 모이는 액을 버렸다. AW1용액 (Wash용액1, QIAamp DNA Blood Mini Kit, QIAGEN) 500㎕를 넣고 8,000rpm에서 1분간 원심분리하여 유출물을 버리고 다시 AW2용액 (Wash용액2, QIAamp DNA Blood Mini Kit, QIAGEN) 500㎕를 넣어 14,000rpm에서 1분간 원심분리하여 유출물을 버렸다. QIAamp 스핀 컬럼을 1.5㎖ 튜브에 옮기고 AE용액(Elution용액, DNA Blood Mini Kit, QIAGEN) 300㎕을 넣어 실온에서 15분간 정치한 후 8,000rpm에서 3분간 원심분리하여 게놈 DNA를 용출하였다. 에탄올(100%) 750㎕를 넣어 -20℃ 에서 1시간 정치한 후 14,000rpm에서 20분간 원심분리한 후 상등물인 에탄올을 버리고 건조시킨 뒤 펠렛은 20㎕ 멸균된 증류수에 녹여 농축시켰다.If the sample was blood, 10 ml blood was sampled into an EDTA tube. The plasma layer was divided into 1.5 ml tubes by centrifugation at 1,800 rpm for 10 minutes, centrifuged at 14,000 rpm for 10 minutes, and the precipitate was divided into 1.5 ml tubes. Suspend in 180µl of lysozyme buffer (20mM Tris-Cl, pH 8.0, 2mM EDTA, 1.2% Triton X-100, 20mg / ml lysozyme) and incubate for 30 minutes at 37 ° C. Lysis solution, QIAamp DNA Blood Mini Kit, QIAGEN) 200μl and gently mixed, incubated for 30 minutes at 55 ℃ and again incubated for 10 minutes at 95 ℃. After adding 200 μl of ethanol (100%) and mixing, the whole solution was transferred to a 2-ml tube containing a QIAamp spin column, and centrifuged at 8,000 rpm for 1 minute to discard the liquid collected in the tube. Add 500 µl of AW1 solution (Wash solution 1, QIAamp DNA Blood Mini Kit, QIAGEN), centrifuge at 8,000 rpm for 1 minute, discard the effluent, and then again 500 µl of AW2 solution (Wash solution 2, QIAamp DNA Blood Mini Kit, QIAGEN). The mixture was centrifuged at 14,000 rpm for 1 minute to discard the effluent. The QIAamp spin column was transferred to a 1.5 ml tube, 300 µl of AE solution (Elution Solution, DNA Blood Mini Kit, QIAGEN) was added thereto, and allowed to stand at room temperature for 15 minutes, followed by centrifugation at 8,000 rpm for 3 minutes to elute genomic DNA. After adding 750 μl of ethanol (100%) for 1 hour at −20 ° C., centrifuging at 14,000 rpm for 20 minutes, discarding the ethanol, which was the supernatant, drying, and pellets were dissolved in 20 μl sterile distilled water and concentrated.

증폭, 혼성화 반응, 세척 및 하이브리드의 검출은 상기 실시예 5 내지 7에서와 같이 동일하게 실시하였다. 그 결과는 하기 표 51에 기재되어 있다. 이 표에 수록된 데이터에서 분자는 검체의 분모는 검체의 적용 횟수이고 분자는 혼성화에 의한 시그널이 나타난 횟수를 가리킨다.Amplification, hybridization reaction, washing and detection of hybrids were carried out in the same manner as in Examples 5 to 7 above. The results are shown in Table 51 below. In the data in this table, the numerator indicates the number of times the sample's denominator is applied and the number of times the molecule shows the signal due to hybridization.

참고로, 도 2, 3, 4, 5, 6, 7 및 8은 스타필로코코스 아우레우스, 슈도모나스 애루지노사, 프로테우스 미라빌리스, 크레브시엘라 뉴모니에, 아시네토박터 바우마니, 이. 콜라이 및 엔테로코코스 페슘 균을 각각 포함한 검체에 대한 블라인드 시험에서 스캔어레이(Scanarray) 5000을 이용하여 검색된 도 1의 DNA 칩에서의 혼성화 반응 결과를 각각 보여준다. 도 10은 스타필로코코스 에피더미디스 균을 포함한 검체에 대한 블라인드 시험에서 스캔어레이 5000을 이용하여 검색된 도 9의 DNA 칩에서의 혼성화 반응 결과를 보여준다. 도 12 및 13은 살모넬라 그룹 E 및 살모넬라 그룹 B 균을 각각 포함한 검체에 대한 블라인드 시험에서 스캔어레이 5000을 이용하여 검색된 도 11의 DNA 칩에서의 혼성화 반응 결과를 각각 보여준다. 도 15 및 16은 크레브시엘라 옥시토카 및 벌코데리아 세파시아 균을 각각 포함한 검체에 대한 블라인드 시험에서 스캔어레이 5000을 이용하여 검색된 도 14의 DNA 칩에서의 혼성화 반응 결과를 각각 보여준다.For reference, FIGS. 2, 3, 4, 5, 6, 7 and 8 are Staphylococcus aureus, Pseudomonas aeruginosa, Proteus mirabilis, Creveciella pneumoniae, Acinetobacter Baumani, Lee . In the blind test on the specimens containing E. coli and Enterococcus fungus, respectively, the results of hybridization on the DNA chip of FIG. 1 searched using Scanarray 5000 are shown. FIG. 10 shows the results of hybridization reactions in the DNA chip of FIG. 9 retrieved using Scanarray 5000 in a blind test on a sample containing Staphylococcus epidermidis bacteria. FIG. 12 and 13 show the results of hybridization reactions in the DNA chip of FIG. 11 retrieved using ScanAray 5000 in a blind test on specimens containing Salmonella group E and Salmonella group B bacteria, respectively. Figures 15 and 16 show the results of hybridization reactions in the DNA chip of Figure 14 retrieved using ScanAray 5000 in a blind test on specimens containing the Crevciella oxytoca and Vulcoderia Sephacia bacteria, respectively.

상기 본 발명에 의하여, 상기 각 병원체의 rRNA 유전자와 특정적으로 혼성화하고 다른 유기체의 핵산과는 교차 반응하지 않는 염기 서열을 갖는 탐침을 개발하였고 또한 이들 탐침을 집적한 DNA 칩을 제작하여 임상 실험을 통해 그들 탐침의 각 특이성을 확인함으로써, 항암치료에 필요한 감염질환을 진단하는 새로운 진단방법을 산업적으로 이용할 수 있을 것이다. According to the present invention, a probe having a nucleotide sequence that specifically hybridizes with the rRNA gene of each pathogen and does not cross-react with nucleic acids of other organisms has been developed. By identifying the specificity of each of these probes, new diagnostic methods for diagnosing infectious diseases for chemotherapy will be available.

도 1은 스타필로코코스 아우레우스, 슈도모나스 애루지노사, 프로테우스 미라빌리스, 크레브시엘라 뉴모니에, 아시네토박터 바우마니, 이. 콜라이 또는 엔테로코코스 페슘 균을 포함한 검체에 대한 블라인드 시험을 위한 DNA 칩의 디자인을 도시한 것이다.1 is Staphylococcus aureus, Pseudomonas aeruginosa, Proteus mirabilis, Crevciella pneumoniae, Acinetobacter Baumani, E. The design of a DNA chip for blind testing on specimens containing E. coli or Enterococcus fungus is shown.

도 2는 스타필로코코스 아우레우스 균을 포함한 검체에 대한 블라인드 시험에서 스캔어레이(Scanarray) 5000을 이용하여 검색된 도 1의 DNA 칩에서의 혼성화 반응 결과를 보여준다.FIG. 2 shows the results of hybridization reactions in the DNA chip of FIG. 1 retrieved using Scanarray 5000 in a blind test on a specimen containing Staphylococcus aureus.

도 3은 슈도모나스 애루지노사 균을 포함한 검체에 대한 블라인드 시험에서 스캔어레이(Scanarray) 5000을 이용하여 검색된 도 1의 DNA 칩에서의 혼성화 반응 결과를 보여준다.FIG. 3 shows the results of hybridization reactions in the DNA chip of FIG. 1 retrieved using Scanarray 5000 in a blind test on specimens containing Pseudomonas aeruginosa. FIG.

도 4는 프로테우스 미라빌리스 균을 포함한 검체에 대한 블라인드 시험에서 스캔어레이(Scanarray) 5000을 이용하여 검색된 도 1의 DNA 칩에서의 혼성화 반응 결과를 보여준다.FIG. 4 shows the results of hybridization reactions in the DNA chip of FIG. 1 retrieved using Scanarray 5000 in a blind test on specimens containing Proteus mirabilis.

도 5는 크레브시엘라 뉴모니에 균을 포함한 검체에 대한 블라인드 시험에서 스캔어레이(Scanarray) 5000을 이용하여 검색된 도 1의 DNA 칩에서의 혼성화 반응 결과를 보여준다.FIG. 5 shows the results of hybridization reactions in the DNA chip of FIG. 1 retrieved using Scanarray 5000 in a blind test on a specimen containing Creebsiella pneumoniae.

도 6은 아시네토박터 바우마니 균을 포함한 검체에 대한 블라인드 시험에서 스캔어레이(Scanarray) 5000을 이용하여 검색된 도 1의 DNA 칩에서의 혼성화 반응 결과를 보여준다.FIG. 6 shows the results of hybridization reactions in the DNA chip of FIG. 1 retrieved using Scanarray 5000 in a blind test on specimens containing Acinetobacter Baumani. FIG.

도 7은 이. 콜라이 균을 포함한 검체에 대한 블라인드 시험에서 스캔어레이(Scanarray) 5000을 이용하여 검색된 도 1의 DNA 칩에서의 혼성화 반응 결과를 보여준다.7 is this. Blind test on specimens containing E. coli shows the results of hybridization reactions on the DNA chip of FIG. 1 retrieved using Scanarray 5000.

도 8은 엔테로코코스 페슘 균을 포함한 검체에 대한 블라인드 시험에서 스캔어레이(Scanarray) 5000을 이용하여 검색된 도 1의 DNA 칩에서의 혼성화 반응 결과를 보여준다.FIG. 8 shows the results of hybridization reactions in the DNA chip of FIG. 1 searched using Scanarray 5000 in a blind test on a specimen containing Enterococcus phenium bacteria.

도 9는 스타필로코코스 에피더미디스 균을 포함한 검체에 대한 블라인드 시험을 위한 DNA 칩의 디자인을 도시한 것이다.Figure 9 shows the design of the DNA chip for the blind test on a sample containing Staphylococcus epidermidis bacteria.

도 10은 스타필로코코스 에피더미디스 균을 포함한 검체에 대한 블라인드 시험에서 스캔어레이 5000을 이용하여 검색된 도 9의 DNA 칩에서의 혼성화 반응 결과를 보여준다.FIG. 10 shows the results of hybridization reactions in the DNA chip of FIG. 9 retrieved using Scanarray 5000 in a blind test on a sample containing Staphylococcus epidermidis bacteria. FIG.

도 11은 살모넬라 그룹 E 균 또는 살모넬라 그룹 B 균을 포함한 검체에 대한 블라인드 시험을 위한 DNA 칩의 디자인을 도시한 것이다.FIG. 11 shows the design of a DNA chip for blind testing of specimens containing Salmonella group E bacteria or Salmonella group B bacteria.

도 12는 살모넬라 그룹 E 균을 포함한 검체에 대한 블라인드 시험에서 스캔어레이 5000을 이용하여 검색된 도 11의 DNA 칩에서의 혼성화 반응결과를 보여준다.FIG. 12 shows the results of hybridization reactions in the DNA chip of FIG. 11 retrieved using ScanAray 5000 in a blind test on specimens containing Salmonella group E bacteria.

도 13은 살모넬라 그룹 B 균을 포함한 검체에 대한 블라인드 시험에서 스캔어레이 5000을 이용하여 검색된 도 11의 DNA 칩에서의 혼성화 반응 결과를 보여준다.FIG. 13 shows the results of hybridization reactions in the DNA chip of FIG. 11 retrieved using ScanAray 5000 in a blind test on specimens containing Salmonella group B bacteria.

도 14는 크레브시엘라 옥시토카 균 또는 벌코데리아 세파시아 균을 포함한 검체에 대한 블라인드 시험을 위한 DNA 칩의 디자인을 도시한 것이다.FIG. 14 shows the design of a DNA chip for blind testing of specimens containing either Crevciella oxytoca or Vulcoderia cephacia.

도 15는 크레브시엘라 옥시토카 균을 포함한 검체에 대한 블라인드 시험에서 스캔어레이 5000을 이용하여 검색된 도 14의 DNA 칩에서의 혼성화 반응 결과를 보여준다.FIG. 15 shows the results of hybridization reactions in the DNA chip of FIG. 14 retrieved using ScanAray 5000 in a blind test on specimens containing Crevciella oxytoca. FIG.

도 16은 벌코데리아 세파시아 균을 포함한 검체에 대한 블라인드 시험에서 스캔어레이 5000을 이용하여 검색된 도 14의 DNA 칩에서의 혼성화 반응 결과를 보여준다.FIG. 16 shows the results of hybridization reactions in the DNA chip of FIG. 14 retrieved using ScanAray 5000 in a blind test on specimens containing Bulcoderia cephacia. FIG.

도 17은 생물학적 시료중의 폴리핵산을 증폭하는데 사용되는 프라이머의 위치를 보여준다. (16S: 16S rRNA; ITS: 내부전사 스페이서 영역 (Internal Transcribed Spacer Region, 이하 "ITS"라 한다); 23S: 23S rRNA).17 shows the location of the primers used to amplify polynucleic acid in biological samples. (16S: 16S rRNA; ITS: Internal Transcribed Spacer Region (hereinafter referred to as “ITS”); 23S: 23S rRNA).

<110> MEDIGENES <120> DNA chips for Detection of Peptostreptococcus anarobius bacilli <130> P05-0674 <160> 428 <170> KopatentIn 1.71 <210> 1 <211> 3510 <212> DNA <213> Acinetobacter baumannii <220> <221> rRNA <222> (1)..(3510) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 1 ccatgggagt ttgttgcacc agaagtagct gcctaactgc aaagagggcg gttaccacgg 60 tgtggccgat gactggggtg aagtcgtaac aaggtagccg taggggaacc tgcggctgga 120 tcacctcctt aacgaaagat tgacgattgg taagaatcca caacaagttg ttcttcatag 180 atgtatctga gggtctgtag ctcagttggt tagagcacac gcttgataag cgtggggtca 240 caagttcaag tcttgtcaga cccaccatga ctttgactgg ttgaagttat agataaaaga 300 tacatgactg atgatgtaag ctggggactt agcttagttg gtagagcgcc tgctttgcac 360 gcaggaggtc aggagttcga ctctcctagt ctccaccaga acttaagaga agttcggatt 420 acagaaatta gtaaatagag atttagatct tggtttatta acttctgtga tttaagtatc 480 acggtattaa gcatgacctg acgaaggcat gtttattcat taacagattg gcaaaattga 540 gtctgaaata aattgttcac tcaagagttt agattaagca attaatctag atgaattgag 600 aactagcaaa ttaactgaat caagcgtttt ggtatatgaa tttagattga agctgtacag 660 tgtttaaata cacagtactt cgaactgtat ggatgaatga gagatcattt gttctgttgc 720 tacaccaact tgtaggtgta acgactgttt ggggttgtat agtcaagtaa ttaagtgcat 780 gtggtggatg ccttggcagt cagaggcgat gaaagacgtg atagcctgcg aaaagctccg 840 gggaggcggc aaatatcctt tgatccggag atttctgaat gggggaaccc acctacttta 900 aggtaggtat tgcaacatga atacatagtg ttgcaaggcg aacgagggga agtgaaacat 960 ctcagtaccc ttaggaaaag aaatcaattg agattccctc agtagcggcg agcgaacggg 1020 gatcagccca ttaagttatg tgtgttttag tggaacgctc tgggaagtgc gaacgtagag 1080 ggtgatattc ccgtacacga aagggcacac ataatgatga cgagtagggc gaggcacgtg 1140 aaaccttgtc tgaatatggg gggaccatcc tccaaggcta aatactcctg actgaccgat 1200 agtgaaccag taccgtgagg gaaaggcgaa aagaacccct gtgaggggag tgaaatagat 1260 cctgaaaccg catgcataca agcagtggga gcaccttcgt ggtgtgactg cgtacctttt 1320 gtataatggg tcagcgactt atattcagta gcaaggttaa ccgtataggg gagccgtagg 1380 gaaaccgagt cttaataggg cgtttagttg ctgggtatag acccgaaacc aggcgatcta 1440 tccatgagca ggttgaaggt tgggtaacac taactggagg accgaaccca ctgtcgttga 1500 aaagccaggg gatgacttgt ggataggggg tgaaaggcta atcaagcctg gtgatagctg 1560 gttctccccg aaagctattt aggtagcgcc tcggacgaat accatagggg gtagagcact 1620 gtttcggcta gggggtcatc ccgacttacc aaaccgatgc aaactccgaa tacctatgag 1680 tactatccgg gagacagact gcgggtgcta acgtccgtag tcaagaggaa aacaatccag 1740 accgccagct aaggccccaa aatcatagtt aagtgggaaa cgatgtggga aggcatagac 1800 agctaggagg ttggcttaga agcagccacc ctttaaagaa agcgtaatag ctcactagtc 1860 gagtcggcct gcgcggaaga tgtaacgggg ctaaaactat gtgccgaagc tgcggatttg 1920 acattagtca agtggtaggg gagcgttctg taagccgatg aaggtgtatt gagaagtatg 1980 ctggaggtat cagaagtgcg aatgctgacg tgagtaacga caaaacgggt gaaaaacccg 2040 ttcgccgaaa gaccaagggt tccagtccaa cgttaatcgg ggctgggtga gtcgacccct 2100 aaggcgaggc cgaaaggcgt agtcgatggg aaattggtta atattccaat acttctgtgt 2160 aatgcgatga gaggacggag aaggttaagt cagcctggcg ttggttgtcc aggtggaagg 2220 atgtaggtat gtatcttagg caaatccggg gtactctata ctgagatccg atagcaagct 2280 gtacttgtac agtgaagtgg ctgataccat gcttccagga aaagtctcta agcttcagtt 2340 acacaggaat cgtacccgaa accgacacag gtggtcaggt cgagtagacc aaggcgcttg 2400 agagaactct gctgaaggaa ctaggcaaaa tggtaccgta acttcgggag aaggtacgct 2460 gttgttggtg atggaacttg cttcctgagc tgacgacagc cgcagaaacc aggccgctgc 2520 aactgtttat taaaaacata gcactctgca aacacgaaag tggacgtata gggtgtgatg 2580 cctgcccggt gctggaaggt taattgatgg ggttagcgta agcgaagctc ttgatcgaag 2640 ccccagtaaa cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt 2700 aagttccgac ctgcacgaat ggcataatga tggcggcgct gtctccagca gaggctcagt 2760 gaaatcgaaa tcgctgtgaa gatgcagtgt acccgcggct agacggaaag accccgtgaa 2820 cctttactgc agcttgacac tgaactttga ccttacttgt gtaggatagg tgggaggctt 2880 tgaagttgga acgctagttc caatggagcc gtccttgaaa taccaccctg gtaatgttga 2940 ggttctaact ctgtcccgtt atccgggacg aggaccgtgt ctggtgggta gtttgactgg 3000 ggcggtctcc tcctaaagag taacggagga gtacgaaggt gcgctcagcg tggtcggaaa 3060 tcacgcgtag agtataaagg caaaagcgcg cttaactgcg agacccacaa gtcgagcagg 3120 tacgaaagta ggtcttagtg atccggtggt tctgtatgga agggccatcg ctcaacggat 3180 aaaaggtact ctggggataa caggctgata ccgcccaaga gttcatatcg acggcggtgt 3240 ttggcacctc gatgtcggct catctcatcc tggggctgaa gcaggtccca agggtatggc 3300 tgttcgccat ttaaagaggt acgcgagctg ggtttagaac gtcgtgagac agttcggtcc 3360 ctatctaccg tgggcgctgg aaatttgaga ggatctgctc ctagtacgag aggaccagag 3420 tggacgaacc tctggtgtac cggttgtgac gccagtcgca tcgccgggta gctatgttcg 3480 gaagggataa ccgctgaaag tttttaagca 3510 <210> 2 <211> 2940 <212> DNA <213> Anaerobiospirillum succiniciproducens <220> <221> rRNA <222> (1)..(2940) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 2 cttccctcag tgattcaaga cagcgcagca tgtgttttgt tcttgattca ttgcagttaa 60 aaagttcttt aaaaatttat cagacaagct tatttaaagt aaaaactcaa ttagcaacta 120 tttaatgggt aaaaagacca aagtcttgta aacctttcgg cagttgttaa aagttacttg 180 atccaatcat gatcaaggcc ttacgacctt tcggggttgt atggtcaagc atgaaagcgc 240 acatggtgga tgccatggcg tcaggaggcg atgaaggacg tgccaatctg cgataagcct 300 aggtaagccg ataaggggcg cttgaaccta ggatttccga atggggaaac ccgacactta 360 gtgtcatcta tgctgaagtt atagaagcga accaggggaa gtgaaacatc tcagtaccct 420 gaggaaaaga aatcaaatca gagattccct cagtagcggc gagcgaacgg ggaacagccc 480 aaaagttgat atgttctagt agaagcagtt gggaaactgc agggcacagg gtgatactcc 540 cgtatacgaa ggagcatatt tgatgagcgt gagtagggcg ggacacgtga tatcctgtct 600 gaatatgggg ggaccatcct ccaaggctaa atactacctg acgaccgata gtgaaccagt 660 accgtgaggg aaaggcgaaa agaaccccgg gaggggagtg aaaaagaacc tgaaaccgtg 720 tgcgtacaag cagtgggagc ctacttgtta ggtgactgcg taccttttgt ataatgggtc 780 agcgagttat ctgtattagc aggttaacct atatggggga gccgtagaga aatcgagtcc 840 taaccggcga atagttgata caggtagacc cgaaactggg tgagctagtc ctggccaggt 900 tgaaagcagg gtaacacctg ccggaggacc gaacccacta acgttgcaaa gttaggggat 960 gagctgggac taggggtgaa aggccaatca aactcagtga tagcttggtt ctcctcgaaa 1020 gctatttagg tagcgttctg cgaggacgta tgggggtaga gcactgttat ggggaagggg 1080 ccccatccgg ggttgccaat ccattgcaaa ctttcgaata ccatacagtt ggaagcagag 1140 gacagacggt gggtgctaac gttcatcgtc aagagggaaa caacccggac cgccagctaa 1200 ggtccccaag ttctagttaa gtgggaaacg atgtgggaag gcatagacag ccaggatgtt 1260 ggcttagaag cagccatcat ttaaagaaag cgtaatagct cactggtcga gtcggcctgc 1320 gcggaagatg taacggggct aaactagaca ccgaagctgc ggattcacac gcaagtgtga 1380 gtggtagagg agcgttctgt aaactgatga aggtgaggcg ggagccgagc tggaggtaac 1440 agaagtgcga atgctgacgt aagtaacgat aaaacacgtg aaaagcgtgt tcgccgaaag 1500 accaagggtt cctgtccaac gttaatcggg gcagggtgag tcggtgccta aggcgaggct 1560 gaaaagcgta gtcgaaggga aacaggtcaa tattcctgta ctttacttaa gtgcgatgag 1620 atgacggaga agggaaagtc agccacttat tggattgtgg tgaaaggctg taggtagata 1680 ccggggttaa aagactggta tcagtgatct gagagctgag acgaagcacc attgtgtgaa 1740 gtgactcgtg cccatgcttc ctggaaaagt ctctaagctt cagcttaagt agagccgtac 1800 cccaaaccga cacaggtggt cgggtagagg ataccaaggc gcttgggaga actcaggtga 1860 aggaactagg caaaattgta ccgtaacttc gggataaggt acgctgttac cggtgagagg 1920 acttgctcct taagctggga acagccgcag tgaaatggtg gctgggactg tttaacaaaa 1980 acacagcact ctgcaaacct gaaaggggaa gtatagggtg tgacacctgc ccggtgctgg 2040 aagattaatt gatggggtgc aagctcctga tcgaagtccc agtaaacggc ggccgtaact 2100 ataacggtcc taaggtagcg aaattccttg tcgggcaagt tccgacctgc acgaatggca 2160 taatgatggc cacactgtct cctcctgaga ctcagtgaag ttgaaatgtt tgtgatgatg 2220 caatctcccc gcggctagac ggaaagaccc catgaacctt tactgcagct ttgcattgga 2280 ctttgaaccg atctgtgtag gataggtggg aggcagtgaa gctaggacgc cagtcttagc 2340 ggagccaacc ttgaaatacc accctgattt gtttgaggtt ctaacctagg agcgtaatcc 2400 gcttcgggga ccgtgcatgg taggcagttt gactggggcg gtctcctcct aaagggtaac 2460 ggaggagtgc gaaggtacgc taggtacggt cggaaatcgt gctgatagtg caatggcaaa 2520 agcgtgcttg actgcgagac cgacaagtcg agcagatacg aaagtaggtc atagtgatcc 2580 ggtggctctg catggaaggg ccatcgctca acggataaaa ggtactctgg ggataacagg 2640 ctgataccgc ccaagagttc atatcgacgg cggtgtttgg cacctcgatg tcggctcatc 2700 tcatcctggg gctgtagccg gtcccaaggg tatggctgtt cgccatttaa agaggtacgt 2760 gagctgggtt taaaacgtcg tgagacagtt ttgtccctat ctgccgtggg cgttggaaga 2820 ttgacggggg ctgctcctag tacgagagga ccggagtgga ctcacctctg gtgtaccggt 2880 tgtcacgcca gtggcatcgc cgggtagcta tgtggggaaa ggataaccgc tgaaattttt 2940 2940 <210> 3 <211> 3379 <212> DNA <213> Bacteroides fragilis <220> <221> rRNA <222> (1)..(3379) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 3 gctctgggag ccgggggtac ctgaagtacg taaccgcaag gatcgtccta gggtaaaact 60 ggtgactggg gctaagtcgt aacaaggtag ccgtaccgga aggtgcggct ggaacacctc 120 ctttctggag cgatgttgaa aacgacgttc attggttcta aattgtacta ctggtacttg 180 tttatttata gaatatagat caaccatcta tagcaatata gatagagata aacaagagaa 240 aaacagaagc cgagtctaaa acaggacacg gttgaactag tcctatagct cagttggtta 300 gagcgctaca ctgataatgt agaggtcggc agttcaactc tgcctgggac taccaacaga 360 tagatatttt atcttgtatg attgggggat tagctcagct ggctagagca tctgccttgc 420 acgcagaggg tcaacggttc gaaatccgtt attctccact ccgataccgc gacctaacag 480 gcttgcgtgt aatcaaacga tctttgacat gatgtacaga aaaaagtaaa tttagtaaga 540 gctaaaagta tatatcgaac catttggttc agatcctttt taagaaaagg agatgaatct 600 ggcgataaaa gaaagtaaat aagggcgcat ggcggatgcc ttggctctcg gaggcgatga 660 angacgtgat aagctgcgat nagcttcggg taggtgcaaa tgacctttga ttccgaagat 720 ttccgaatgg gacaaccccg gcattctgaa ggaatgtcat ccaacttggt tggaggctaa 780 cgcagggaac tgaaacatct tagtacctgc aggaaaagaa aataaataat gattccccta 840 gtagtggcga gcgaacgggg attagcccaa accacccatg ttacggcatg tgtggggttg 900 taggaccacg ctctcgcaag acagttattg agaagaaacg actggaaagt cgtgccatag 960 acggtgatag cccggtattc taaggtaacc gaagcgtagt ggtatcctga gtagcgcgga 1020 gcacgaggaa ttctgcgtga accagccggg accatccggt aaggctaaat actcccgaga 1080 gaccgatagc gaaccagtac tgtgaaggaa aggtgaaaag cacttcgaat agaagagtga 1140 aatagtccct gaaaccatgc gcctacaagc ggtcggagca gcttaagctg tgacggcgtg 1200 ccttttgcat aatgaaccta cgagttactt tttccggcaa ggttaagcat cttgagatgt 1260 ggatccgaag cgaaagcgag tctgaacagg gcggatagtc ggaaggagta gacgcgaaac 1320 caagtgatct acccttgggc aggttgaagg ttaggtaaca ctaactggag gaccgaaccg 1380 ataagcgttg aaaagcttcc ggatgacctg agggtggggg gtgaaaggct aatcaaactt 1440 ggagatagct cgtactcccc gaaatgcatt taggtgcagc cttgagagtt actaatgtga 1500 ggtagagcga ctgataagat gcgagggctt caccgcctat caagtcttga taaactccga 1560 atgcgcatta gttctatctc aggagtgagg gcatgggtgt aaggtccatg tctaaagaga 1620 agaatccaga ccatcagtaa ggtccccaaa taaacattaa gttgacctaa cgaagtcaga 1680 ttgctaagac agctaggatg ttggcttgga agcagccatt catttaaaga gtgcgtaaca 1740 gctcactagt cgaggagttt ggcgtggata ataatcgggc ataagtgttt taccgaagct 1800 atgggatccg tatggatcgg taggggagca ttccactcag cgttgaaggc gaagcgtgag 1860 ctttactgga gcgtgtggaa aagcaaatgt aggtataagt aacgataaag ggggtgagaa 1920 accccctcgc cgcaagacta aggtttcctg atcaacgcta atcggatcag ggttagtcgg 1980 gtcctaaggc tcagccgaac ggtgaggccg atggcagaac aggttaatat tcctgtacta 2040 cctataagag tgatgtggag acggaggagt gacaacgccg cggactgacg gaatagtccg 2100 ttaaagggtg tagatgttga ttatcccagg caaatccggg ataagagtcg aacctgacag 2160 tataccaatt tcctcggaaa acggtaatag tgcgtgtaaa catactccca agaaaatccg 2220 ctaaacttaa tcttataggt acccgtaccg taaacggaca cacgtagtcg ggttgaatat 2280 actcaggcgc ttgagtgaat cacggttaag gaactaggca aattgaccct gtaacttcgg 2340 gataaagggt ccctacccgg tgacggggag ggcgcagaaa aataggtcca ggccaacctg 2400 tttaacaaaa acacagggct gtgcaaaatt gaaagatcac gtttacagcc ctgacacctg 2460 cccggtgctg gaaggttaag aggagatgtc atcgcaagag aagcattgaa ttgaagcccc 2520 agtaaacggc ggccgtaact ataacggtcc taaggtagcg aaattccttg tcgggtaagt 2580 tccgacctgc acgaatggcg taacgatggc cacactgtct ccaccagaga ctcagcgaag 2640 ttgaaatgtt tgtaatgatg catctccccg cggaaagacg gaaagacccc atgaaccttt 2700 actgtagctt tgtattggat tttgaacgga tctgtgtagg ataggtggga ggctttgaag 2760 tgaggacgct agttctcatg gagccgacgt tgaaatacca ccctggtgcg ttgaggttct 2820 aacccaggtc ccttatcggg atcggggaca gtgcatggta ggcagtttga ctggggcggt 2880 ctcctcccaa agcgtaacgg aggagttcga aggtacgcta gttacggtgc ggacatcgtg 2940 acgatagtgc aatggcataa gcgtgcttaa ctgcgagact gacaagtcga gcagatgcga 3000 aagcaggaca tagtgatccg gtggttctgt atggaagggc catcgctcaa cggataaaag 3060 gtactctggg gataacaggc tgataccgcc caagagttca tatcgacggc ggtgtttggc 3120 acctcgatgt cggctcatct catcctgggg ctgtagtcgg tcccaagggt atggctgttc 3180 gccatttaaa gaggtacgtg agctgggttt aaaacgtcgt gagacagttt ggtccctatc 3240 ttccgtgggc gctgcagatt tgaggaagcc tgctcctagt acgagaggac cggagtggac 3300 acacctctgg tgtatcggtt gtcacgccag tggcattgcc gagtagctaa gtgtggaaga 3360 gataaccgct gaacttttt 3379 <210> 4 <211> 3138 <212> DNA <213> Cardiobacterium hominis <220> <221> rRNA <222> (1)..(3138) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 4 cccctgataa gggtgaggtc aaagttcaat ttttcagacc ccccaaaaag ggccatagct 60 cagctggaga gcgcctgctt ttgcacgcag agtcagagtt cgatcctcct gggctcccca 120 tttgaaacgt agtttgctaa agagagcaga aaattgaaca tgaagctaaa gctgatgtaa 180 gaacacagat ggagcacatg gtgttcaatt ttttgttgtc ttaggacaat gatctataac 240 aaccggaaac attcaattta aacaactaaa ttgagtagct gagaaaaaca agacgttgtt 300 acttatgttt tcctctgcgt agccaacaga tgatgagaga gcaaggcgaa agcgaagcat 360 acttcagaat ctgaaaacgt agagtagtaa agagagaaca gcacaaccga aagataactt 420 gaggttgtat gatcaagtga ataagtgcac acggtggatg ccttggcgac aacaggcgaa 480 gaaggacgtg gaaatctgcg aaaagccccg ggaagctgat aacaagcaat gatccgggga 540 tctccgaatg gggcaacccg accttagggt catccgtagt tgaatacata gactgcggaa 600 gcgaaccggg agaactgaaa catctaagta ccccgaggaa aagaaatcaa ccgagattcc 660 gcaagtagcg gcgagcgaac gcggagaagc ccaattaagt tgcgtatgag ctagtagaac 720 atcctgggaa gggtggccat agagggtgat agccccgtat acgaaagcca tacacaatga 780 atcgagtagg gcgggacacg tgaaatcctg tctgaagatg gggggaccat cctccaaggc 840 taaatactcg ttgtcgaccg atagtgaacc agtaccgtga gggaaaggcg aaaagaaccc 900 tggtgaaggg agtgaaatag aacctgaaac cgtgtgcata caagcagtca gagcatatga 960 agatatgtga tggcgtacct tttgtataat gggtcagcga gttacattca gtggcgaggt 1020 taaccgaata ggggagccgt agcgaaagcg agtcttaata gggcgataag tctctgggtg 1080 tagacccgaa accgggcgat ctatccatgg gcaggttgaa ggttgagtaa catcaactgg 1140 aggaccgaac ccacgcatgt tgaaaaatgc ggggatgacc tgtggatagg agtgaaaggc 1200 taaacaagct cggagatagc tggttctccc cgaaaactat tgaggtagtg ccttgtagat 1260 tgacttacgg gggtagagca ctgtttcggc tagggggtca taccgactta ccaacccgat 1320 gcaaactcga ataccgtaaa gttttactac aggagacaca cggcgggtgc taacgtccgt 1380 cgtgaagagg gaaacaaccc agaccgccag ctaaggtccc gaaatgacag ttcagtggga 1440 aacgaagtgg gaaggcttag acagctagga ggttggctta gaagcagcca tcctttaaag 1500 aaagcgtaat agctcactag tcgagtcgtc ctgcgcggaa gatttaccgg ggctaaactg 1560 tctaccgaag ctgcggattt gcatgaagat gcaagtggta ggggagcgtt gtgtaagccg 1620 aagaaggtgt gttgagaagc atgctggagg tatcacaagt gcgaatgctg acatgagtaa 1680 cgataatgcg ggtgaaaaac ccgcacaccg aaaacccaag gtttcctgcg caacgctaat 1740 cggcgcaggg tgagtcggcc cctaaggcga ggcggaaacg cgtagtcgat gggaaacagg 1800 ttaatattcc tgtactgatc ataattgcga tggggggacg gagaaagcta ggccagcaca 1860 ctgttggatg tgtgtttaag cgcgtaggtt ggggacttag gcaaatccgg gttcctaaga 1920 ctgaaacgtg atgacgagtt tctacggaaa cgaagtggtt gatgctctgc ttccaggaaa 1980 agcctctaag cttcagatta tgacaaccgt accccaaacc gacacaggtg ggtaggaaga 2040 gaattccaag gtgcttgaga gaactcgggt aaaggaactc ggcaaaatga taccgtaact 2100 tcgggataag gtatgcctct gatggtgaat agctgttgga ggccgcagag aataggccgc 2160 tgcgactgtt tatcaaaaac acagcactct gcaaacacgc aagtggacgt atagggtgtg 2220 acgcctgccc ggtgccggaa ggttaattga tggggtgcaa gctcttgatc gaagccccgg 2280 taaacggcgg ccgtaactat aacggtccta aggtagcgaa attccttgtc gggtaagttc 2340 cgacctgcac gaatggcgta acgatggcgg cgctgtctct acccgagact cagtgaagtt 2400 gaaatcgctg tgaagatgca gtgtacccgc ggcaagacgg aaagaccccg tgaaccttca 2460 ctacagcttg acactgaaca ttgaaataag ttgcatagga taggtgggag gctttgaagc 2520 agagactctg gtttctgtgg agccgacctt gaaataccac cctgcttatt ttgatgttct 2580 aacgtagacc cgtaatccgg gttgcagaca gtgtctggtg ggtagtttga ctggggcggt 2640 ctcctcccaa agagtaacgg aggagtgcga aggtgggctc ggtgcggtcg gaaatcgcac 2700 caagagtgca aaggcagaag cccgcttaac tgcgagacag acaagtcgag cagatacgaa 2760 agtaggtctt agtgatccgg tggttctgaa tggaagggcc atcgctcaac ggataaaagg 2820 tactccgggg ataacaggct gattcctccc aagagtccat atcgacggag gagtttggca 2880 cctcgatgtc ggctcatcac atcctggggc tgtagcaggt cccaagggta tggctgttcg 2940 ccatttaaag tggtacgcga gctgggttca gaacgtcgtg agacagttcg gtccctatct 3000 gccgtgggcg ttggagattt gagggaagct gttcttagta cgagaggacc ggaatggacg 3060 aacctctggt gtaccggttg tcacgccagt ggcacagccg ggtagctatg ttcggaaggg 3120 ataaccgctg aacttttt 3138 <210> 5 <211> 3332 <212> DNA <213> Chryseobacterium meningosepticum <220> <221> rRNA <222> (1)..(3332) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 5 ctctggagct gggggtacct gagtcggtga ccgtaaagga gctgcctagg gtaaaactag 60 taactagggc taagtcgtaa caaggtagcc gtaccggaag gtgcggctgg aacatctcat 120 tttagagcgt cctttggacg ataaacaaaa caaagatcac ttaggtgatc acttacttaa 180 acaaagcaca gctttggttt tactttggtt gctattacaa aagataaccc cttagattag 240 taacagggaa agagatagtt tataattatg aattttaatg aataattata aattaatgaa 300 gtctcgtagc tcagctggtt agagcgctac actgataatg tagaggtcgg cagttcgagc 360 ctgcccgaga ctactaattt aaaagatttg aagttagaga ttggaagttt gaagagataa 420 tttcaaacct caaactaaga atttcaaatt taagaggggg aattagctca gctggctaga 480 gcgcctgcct tgcacgcagg aggtcaaggg ttcgactccc ttattctcca cagttttaga 540 agagagacac aagtatacga atagagccaa taacaatatt cgtttgttga tcagaagaaa 600 agaacaaaag atcattgaca ttaacggtaa agacatcaca aagagataac cgagcactcc 660 ttgagtgcag agtaaaaaat aaattaatta ttaggaaaga aatcgttaag ggcgtatggc 720 ggatgcctag gctttcagag gcgacgaagg acgtggtaag ctgcgaaaag ctacggggat 780 tggcacacac gaattgatcc gtagatatcc gaatggggca acccggcata ttgaagatat 840 gtcacccagc aatgggagca aacccggaga actgaaacat ctaagtaccc ggaggaaaag 900 aaatcgaaga gattccgtaa gtagtggcga gcgaaagcgg attagcccaa aagtctttat 960 atgtttagaa gaacacactg gaaagcgtgg ccatagaggg tgatagcccc gtatttgaaa 1020 ggcatattta gatgataaat gagtagggcg ggacacgtga aatcctgtct gaatatgggg 1080 ggaccatcct ccaaggctaa atactcctga aagaccgata gtgaacaagt actgtgaagg 1140 aaaggtgaaa agcacttcga atagaagggt gaaatagaac ctgaaaccgt acgcctacaa 1200 gcggtcggag cagctataag ctgtgacggc gtgccttttg cataatgagc ctacgagtta 1260 atcttactag cgaggttaag gacttaaggt ccggagccgt agcgaaagcg agtctgaata 1320 gggcggatag ttagtgggat tagacgcgaa accttgtgat ctacccatgg gcaggttgaa 1380 gctttggtaa cacaaagtgg aggaccgaac cggttgacgt tgaaaagtct tcggatgacc 1440 tgtgggtagg ggtgaaaggc caatcaaact gggagatagc tcgtactccc cgaaatgcat 1500 ttaggtgcag cgtcgattta gtttattaga ggtagagcta ctgattggat gcgggggttt 1560 catcgcctac caattcctga caaactccga atgctaataa atgtaacttg ggagtcagaa 1620 catgggtgat aaggtccgtg ttcgaaaggg aaacagccca gaccaccagc taaggtccca 1680 aaatatatgt taagtggaaa aggatgtggc gttgcccaga caactaggat gttggcttag 1740 aagcagccat catttaaaga gtgcgtaata gctcactagt cgagtgatcc ggcatggata 1800 ataatcgggc ataagcatat taccgaagct atggcagtat ttatactggg taggggagca 1860 ttctatttgc gccgaagcag tactgtgagg tattgtggag cggatagaaa agaaaatgta 1920 ggcataagta acgataaagg gggcgagaaa ccccctcacc gaaagactaa ggtttcctca 1980 gccatgctaa tcagctgagg gttagtcggg gcctaacgcg aagccgaatg gcgtagtgga 2040 tggacaacgg gttaatattc ccgtaccagt atattaataa aagtgacgga gttggaaact 2100 aggtgcgtac tgacggaata gtacgttgaa gtaatcgtga ggttacgata gtacagcaaa 2160 tcttcggatg cgctgataat cctggggacc cgcttccaag aaaagcgaaa tatactgccc 2220 gtaccaaaac cgacacaggt agtcgaggag agaatcctaa ggtgctcgag tgagtcgtgg 2280 ctaaggaact aggcaaaata gtctcgtaac ttcggaagaa gagacgcctc cctccgggga 2340 ggccgcagtg aagaggccca ggcgactgtt tatcaaaaac acaggactct gctaaatcga 2400 aagatgctgt atagggtctg acacctgccc ggtgctggaa ggttaaggaa ggtgcttaga 2460 gttaaatcga aggcattaac tgaagcccca gtaaacggcg gccgtaacta taacggtcct 2520 aaggtagcga aattccttgt cgggtaagtt ccgacctgca cgaatggtgt aacgatgctg 2580 ggcactgtct cagccacgag ctcggtgaaa ttgtagtatc ggtaaagatg ccgattaccc 2640 gcaatgggac gaaaagaccc tgtgaccctt tactataact tcgtattgac ttcgagtaaa 2700 caatgtgtag gataggtgga ggctatgaag caggcacgct agtgtttgtg agccaacgtt 2760 gaaataccac cctttgttta cttggagcct aacttctacg gaaggacatt gcgtggtggg 2820 tagtttgact ggggtggtcg cctccaaaag agtaacggag gctttcaaag gtaccctcag 2880 cacgcttggt aaccgtgcgt agagtgtaat ggcataaggg tgcttgactg tgagacctac 2940 aagtcgatca ggtgcgaaag caggacatag tgatccggtg gttccgtatg gaagggccat 3000 cgctcatagg ataaaaggta ctccggggat aacaggctag tctcccccaa gagctcacat 3060 cgacggggag gttcggcacc tcgatgtcgg ctcgtcacat cctggggctg gagaaggtcc 3120 caagggttgg gctgttcgcc cattaaagtg gcacgcgagc tgggttcaga acgtcgtgag 3180 acagttcggt ctctatctat tgcgggcgtt agatgtttga gagggcttga ttctagtacg 3240 agaggaccga attgaacaaa cctctggtgt atcagttgta ccgccaggtg caccgctaaa 3300 tagctatgtt tggaagagat aagcgctgaa ag 3332 <210> 6 <211> 3252 <212> DNA <213> Clostridium ramosum <220> <221> rRNA <222> (1)..(3252) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 6 gcaccgctat agtttgtaac acccgaagtc ggtgaggtaa ccttttggag ccagccgccg 60 aaggtgggaa agaactgatt ggggatgata ttgtctttgt aacaagggta gccgtatccg 120 gaaggtgcgg ctggatcacc tcctttctaa ggaaattata cggaatgatc gaagacctga 180 tgcggtctta tagacaggtg cgtttngtag atacttgttt agttttgaag tgatcattcc 240 ttcaaagcgt atgactcaga gatcattgat tactggataa gtagacaatc acattgcaat 300 agaaatagca aaatnagatc gatcatagta attgtctata ttgacgggtt acgttactag 360 aaaacgcact gaaactaagt attttctcct aaaacataaa tcgaacggat ggatggattt 420 tagtaaatag ttgatgatag taatcaagca acaccatata ggtaagtaag aaaaggtatg 480 gcggatgcct ggcacagaga gcgaagaagg acgcagcaac agcaaatgtg acggttagca 540 gtgagcatgg cttatctgtg gatgtctgaa taggggaacc cccctggagt agtagtccag 600 cgtatcctca agtgaatata tagcttcaga gaggcgagac tcaggggaac ctgaaacaat 660 ctaaggtcct caggaaggag aggagaaaat aacacgaatg attccctaag tatcggcgag 720 cgaaagggga agagcccaaa ccgatcttag gatggggttg taggactgtc ggcaaagagc 780 aagaaatcat tataggcgaa cggaatggga agtccggcga aacagggtga cagccccgta 840 gccgaaatag tgaagaagca cgagacagca cctgagtacg gcgggacacg aggaatccct 900 ccttgtcgtg aatccaccag gaccttctgg taaggctaaa tactaccttc tgtgaccgta 960 tagtgaacca ataccgtgag ggaaaggtga aatagacccc cgggaagggg agtgaaatag 1020 atcctgaaac cgtatgctta caagaagtta gagcccgtta aagggtgata gcgtgccctt 1080 ttgtagaatg aaccggcgag ttacgatatg gagcgaggtt aagcaggaat atgccggagc 1140 cgaagcgaaa gcgagtttta acaggggcga aagttgcatg tcgtagaccc gaaaccgagt 1200 gatctagcca tgaccaggtt gaagttgggg taaaacccga tggaggaccg aaccgacccc 1260 ccgttgaaac gttgtgcgga tgatgttggt ggctagtggg tgaaattcca atcgtaactc 1320 ggagatagct tggttctccc cgaaatagct ttagggctag cagtcgtagt gtaaagtcag 1380 tgtgaaggta gagcactgta atatgtgatg gccccatctc ggggtactga acataatcaa 1440 actctcgaat gtgcacaaag atatactcgg cagtcagaca gtgggtgata aggtccattg 1500 tcaaaaaggg aaacagccca gaccatcagc taaggtccca aaatatatac taagtggaaa 1560 aggatgtgga gatgtccaga caactaggca ggttggctca gaatgcagcc attcaacctt 1620 taatagagtg cgtaacagct cactagtcga agtgactctg cgccgataat ttatccgggg 1680 ctaagtatat ttaccgaagc tatggattta cgcgttaaag cgtgagtggt tagggggagc 1740 gttctatgtg cggagaagcg gtaccgtaag gagccgtgga gcgcatagaa gagagaatgc 1800 cggtgtgagt agcgaaacgt gggtgagaat cccacgccac cgaaaaccca aggtttccag 1860 aggaaggttc gtccgcctca gggtaagtcg ggacctaagg cgaggccgag aggcgtagtc 1920 gatggacaac aggtagagat tcctgtactt acggtatgaa tgatggagtg acggagaagg 1980 ctagcggatc ctgctgatgg aaatgcaggt gcaagcgagg gagccgccag ccaggcaaat 2040 ccggctgtcg aaaggccatg gcgctgaggc gtatggaaag ctgcggcaag tacagaagtc 2100 cgtgaagcca gcttccaaga aaagcttcta gtgataatca tacagtaacc cgtaccgaaa 2160 atggacacac atgggtgagg agagaatacc taaggtgagc gagagaacta tagctaagga 2220 actctgcaaa atgactccgt aacttaggga taaggagtgc tcatagagat atgagccgca 2280 gtgaaacggc ccaagcgact gtttaccaaa aacacagctc tatgctaagt cgaaagacga 2340 cgtatatggg gtgacgcctg cccggtgctg gaaggttaag aggatgtgtc agcgcaagcg 2400 aagcattgaa ttgaagcccc agtaaacggc ggccgtaact ataacggtct aagagggaat 2460 tcctcaggta agttaccgcc ccaaagagag cgatttgtgg tgttcatctg tagatggtga 2520 agtattagta cctgtcaaga gcaggttacc ccccctagac ggaaagaccc catggagctt 2580 tactgtagct tgatattgga ttctttgatg caaagatgta caggcatagg taggagtatg 2640 agagacatgc acgccagtgt gtgaggagtc aatgttggga tactactctt ccttgtattg 2700 gagttgctaa ccggatgcca tggaactggc aacgggcaca gtagtcaggt cgggcagttt 2760 gactggggcg gtcgcctccc aaagagtaac ggaggcgccc aaagataccc tcagcttgga 2820 tggaaatcaa gcgcagagtg caaaggcata agggtgtttg actgcgagac ctacaagtcg 2880 agcagggacg aaagtcgggc ttagtgatcc ggcggtgctg aatggaaagg ccgtcgctca 2940 acggataaaa gctaccctgg ggataacagg ctgatctccc ccaagagttc acatcgacgg 3000 ggaggtttgg cacctcgatg tcggctcatc gcatcctgga gctgaagtcg gttccaaggg 3060 ttgggctgtt cgcccattaa agcggtacgc gagctgggtt cagaacgtcg tgagacagtt 3120 cggtccctat ctgtcgtggg cgtaggaagt ttgagaagat ctgtcctcag tacgagagga 3180 ccgggaggac atatcaatgg tgcaccagtt gtcacgccag ggcacagcgg tagctaaata 3240 ggaagggaaa aa 3252 <210> 7 <211> 3531 <212> DNA <213> Comamonas acidovorans <220> <221> rRNA <222> (1)..(3531) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 7 ctcatgggag cgggtctcgc cgagtaggta gcctaaccgc aaggagggcg cttaccacgg 60 cggggttcgt gactggggtg aagtcgtaac aaggtagccg tatcggaagg tgcggctgga 120 tcacctcctt tctggaaaac tgctgttcaa gttgaacgcc cacacttatc ggttgttgga 180 acaagccatg tgccctggtt gcagggtggg tggactgggt ctgtagctca gctggttaga 240 gcaccgtctt gataaggcgg gggtcgttgg ttcgagccca actagaccca ccaagattcc 300 aatatctggt tgtcgaggat cccgggggat tagctcagct gggagagcac ctgctttgca 360 agcagggggt cgtcggttcg atcccgtcat cctccaccaa gatcgcgctg gtggcagcag 420 cttgaaaaag cagctggagg aagcgaaaag agcacgaaaa aagcgtgcta taatatttga 480 ctcaacacta aagcagtctc gtgtgagact gctttagtgt tgatagttat ctaactatca 540 atcggctgtt ctttaaaaat tcatagagtc gaaatcagcg ttgctgacgg aaagagattt 600 caatctcacc gtgccgtcag caacattttg attgcgtcaa aacgaatgaa actttgtttt 660 attcaagtaa tgacgaattg ttctcttgac agaaatgtca aagaattcat tcacattacg 720 gcataacgcg cgaggtgaga gacctcgcaa gtccttgaaa gaaaacggcg aaatctcgca 780 agagagatca aagttatagg gtcaagtgac taagagcatg tggtggatgc cttggcgatg 840 ataggcgacg aaagacgtga tagcctgcga taagcttcgg ggagctggca aattagcttt 900 gatccggaga tttctgaatg gggaaaccca cccgcaaggg tatcgcatac tgaatacata 960 ggtatgcgag gcgaacctgg agaactgaaa catctaagta cccagaggaa aagacatcaa 1020 ccgagattcc gaaagtagtg gcgagcgaaa tcggaagagc cttctagtga tagtcagacg 1080 gttaacaaaa cggaatggaa agtccggcca tagtaggtga tagccctgta tgtgaaaacc 1140 gactggtggt actgagctag agaaaagtag ggcggggcac gagaaaccct gtctgaatat 1200 ggggggacca tcctccaagg ctaaatactc atcatcgacc gatagtgaac cagtaccgtg 1260 agggaaaggc gaaaagaacc ccgggagggg agtgaaatag atcctgaaac cgcatgctta 1320 caaaaagtag gagcccgcaa gggtgactgc gtaccttttg tataatgggt cagcgactta 1380 cattcagtgg caaggttaac cgaatagggg agccgtagag aaatcgagtc cgaatagggc 1440 gtccagtcgc tgggtgtaga cccgaaacca agtgatctat ccatggccag gatgaaggtg 1500 ccgtaacagg tactggaggt ccgaaccgac tagtgttgca aaactagcgg atgagctgtg 1560 gataggggtg aaaggctaaa caaacttgga aatagctggt tctctccgaa aactatttag 1620 gtagtgcctc aagtattacc tgcgggggta gagcactgtt taggctaggg ggtcatggcg 1680 acttaccaaa cctatgcaaa ctccgaatac cgcagagtac agcttgggag acagagcacc 1740 gggtgctaac gtccggactc aagagggaaa caacccagac cgccagctaa ggtccctaaa 1800 attggctaag tgggaaacga agtgggaagg ctaaaacagt caggatgttg gcttagaagc 1860 agccatcatt taaagaaagc gtaatagctc actgatcgag tcgtcctgcg cggaagatgt 1920 aacggggcta agccagttac cgaagctgcg gatttgcaat ttattgcaag tggtaggaga 1980 gcgttctgta agcctgtgaa ggtgcctggt aacgggtgct ggaggtatca gaagtgcgaa 2040 tgctgacatg agtagcgtta aagggggtga aaagccccct cgccgtaagc gcaaggtttc 2100 ctacgcaacg ttcatcggcg tagggtgagt cggcccctaa ggcgaggcag agatgcgtag 2160 ctgatgggaa acaggtcaat attcctgtac cgatgtgtag tgcgatgtgg ggacggagaa 2220 ggttagctca gccaactgtt ggatatgttg gttcaagcct gtagtcgtgc ctggtaggca 2280 aatccgccgg gcttagatga ggggtgataa cgagtctgct tgcagacgaa gtgagtgata 2340 ccctgcttcc aggaaaagcc actaagcttc agctacacac gaccgtaccg caaaccgaca 2400 ctggtgcgcg agatgagtat tctaaggcgc ttgagagaac tctggagaag gaactcggca 2460 aattgatacc gtaacttcgg gagaaggtat gccgcaagta ggtgaacttg aacaaaggga 2520 gcccaaagcg gttgcaaaaa atcggtggct gcgactgttt aataaaaaca cagcactctg 2580 caaacacgaa agtggacgta tagggtgtga cgcctgcccg gtgctggaag attaaatgat 2640 ggggtgcaag ctcttgattg aagtcccagt aaacggcggc cgtaactata acggtcctaa 2700 ggtagcgaaa ttccttgtcg ggtaagttcc gacctgcacg aatggcgtaa cgatggccac 2760 actgtctcct ccagagactc agcgaagttg aaatgtttgt gatgatgcaa tctccccgcg 2820 gaaagacgga aagaccccat gaacctttac tgtagctttg tattggattt tgaacggatc 2880 tgtgtaggat aggtgggagg ctttgaagtg aggacgctag ttctcatgga gccgacgttg 2940 aaataccacc ctggtgcgtt tgaggttcta acccaggtcc cttatcggga tcggggacag 3000 tgcatggtag gcagtttgac tggggcggtc tcctcccaaa gcgtaacgga ggagttcgaa 3060 ggtacgctag ttacggtcgg acatcgtgac gatagtgcaa tggcataagc gtgcttaact 3120 gcgagactga caagtcgagc agatgcgaaa gcaggacata gtgatccggt ggttctgtat 3180 ggaagggcca tcgctcaacg gataaaaggt actctgggga taacaggctg ataccgccca 3240 agagttcata tcgacggcgg tgtttggcac ctcgatgtcg gctcatctca tcctggggct 3300 gtagtcggtc ccaagggtat ggctgttcgc catttaaaga ggtacgtgag ctgggtttaa 3360 aacgtcgtga gacagtttgg tccctatctt ccgtgggcgc tgcagatttg aggaagcctg 3420 ctcctagtac gagaggaccg gagtggacac acctctggtg tatcggttgt cacgccagtg 3480 gcattgccga gtagctaagt gtggaagaga taaccgctga aattttaacc a 3531 <210> 8 <211> 3200 <212> DNA <213> Corynebacterium diphtheriae <220> <221> rRNA <222> (1)..(3200) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 8 acaccgcgcc gtcacaccac gcagcagttt gtaacacccc gagtcggtag ggtaaccctt 60 tatggagcca gccgccgaag gtgggacaga taattggggt gaagtcgtaa caaggtagct 120 gtatcggaag gtgcggctgg atcacctcct ttctaaggaa aaggaaacct gtgagttttc 180 gttcttctct atttgttcag ttttgagagg ttagtacttc tcagtatgtt tgttctttga 240 taactagata agaaagttag taaagttagc atagataatt tattatttat gacacaagta 300 accgagaatc atctgaaagt gaatctttca tctgtattgg aagtatcatc gctgatacgg 360 aaaatcagaa aaacaacctt tacttcgtag aagtaaattg gttaagttag aaagggcgca 420 cggtggatgc cttggcacta ggagccgaag aaggacggga ctaacaccga tatgctttgg 480 ggagctgtac gtaagcgttg atccagagat ttccgaatgg gggaacccac tatctttagt 540 cggatagtat ccttacgtga atacatagcg tgaggaaggc agacccaggg aactgaaaca 600 tctaagtacc tggagcaaga gaaagaaaaa tcgatttcct gagtagcggc gagcgaaacg 660 gaaagagccc aaaccaagaa gcttgcttct tggggttgta ggacacttct atacggagtt 720 acaaaagaaa gttataaatg aagcggtctg gaaaggcccg ccaaaagacg ggtaacagcc 780 cggtagttga aatggctttc cctccccaga gtggatcctg agtacggcgg aacacgtgaa 840 attccgtcgg aatccgggag gaccatcttc ccaaggctaa atactcccct agtgaccgta 900 tagtgaaccc agtaccgtga gggaaaggtg aaaagcaccc ccgcaagggg cagtgaaaca 960 gttcctgaaa ccgtgtgcct acaagtagtt agagcccgtt aatgggtgat agcgtgcctt 1020 ttgtagaaat gaaccggcga gttacgattt gttgcaaggt taagcggaaa aagcggagcc 1080 gtagcgaaag cgagtctgaa tagggctgca taagtaacag gtcgtagacc cgaaaccagg 1140 tgatctaccc atgtccagga tgaaggtaag gtaatactta ctggaggtcc gaacccacgc 1200 acgttgaaaa gtgcggggat gaggtgtggg tagcggagaa attccaatcg aacttggaga 1260 tagctggttc tctccgaaat agctttaggg ctagcctcga ggtaaagagt catggaggta 1320 gagcactgtt tggactaggg gcccttctcg ggttaccgaa ttcagataaa ctccgaatgc 1380 catgtactta tactcgggag tcagactgcg agtgataaga tccgtagtcg aaagggaaac 1440 agcccagacc accagttaag gtccccaaat atatgttaag tggaaaagga tgtggggttg 1500 cttagacaac caggatgttg gcttagaagc agccaccatt gaaaattgaa gtgcgtaata 1560 gctcactggt ccgagtgacc ccgcgccgaa aatgtaccgg ggctaaacat attaccgaaa 1620 ctgtggatga acctctttag aggttcgtgg taggagagcg ttctaagggc ggtgaagtca 1680 gaccggaagg actggtggag cgcttagaag tgagaatgcc ggtatgagta gcgaaagaag 1740 ggtgagaatc ccttccaccg aatatctaag gtttcctgag gaaggctcgt ccgctcaggg 1800 ttagtcggga cctaagccga ggccgatagg cgtaggcgat ggacaacagg tagagattcc 1860 tgtaccagtg ctaattgttt aaccgatggg gtgacacaga aggataggga atcgcacgaa 1920 tggaaatgtg cgtccaagca gtgagtgtga gaagtaggca aatccgcttc tcgcgaagca 1980 tgagctgtga tggggaagga aattaagtac ggaagttcct gatttcacgc tgtcaagaaa 2040 agcctctagg aagagtagta ctgcccgtac cgcaaaccga cacaggtaga tgaggagaga 2100 atacctaagg tgagcgagag aacaatctcg ttaaggaact cggcaaaatg accccgtaac 2160 ttcgggagaa ggggtgctct attagggtgc aagcccgaga gagccgcagt gaataggccc 2220 aggcgactgt ttagcaaaaa cacaggtctc tgcaaaaccg taaggtgacg tataggggct 2280 gacgcctgcc cggtgctgga aggttaagag gagtgcttag cttcggcgaa ggtacgaatt 2340 gaagccccag taaacggcga gaccgtaact ataacgggtc ctaaaaggta gcgaaattcc 2400 ttgtcgggta agttccgacc cgcacgaaag gcgcaacgat ctgggcactg tctcaacgag 2460 agactcggtg aaattatagt acctgtgaag atgcaggtta cccgcgacag gacggaaaga 2520 ccccgtggag ctttactgca acctgatatg gaatgtttgt accgcttgta caggataggt 2580 aggagccgaa gagacgtgtg cgctagcata cgaggaggca atggtgggat actaccctgg 2640 ctgtatgacc attctaaccc gccacgctta ggcgcgtggg gtagacaagt gtcaggtggg 2700 cagtttgact ggggcggtcg cctcctaaag agtaacggag gcgcccaaag gttccctcag 2760 aatggatgga aatcattcgc agagtgtaaa ggcacaaggg agcttgactg cgagactgac 2820 aagtcgagca gggacgaaag tcgggcttag tgatccggtg gttccgcatg gaagggccat 2880 cgctcaacgg ataaaagcta ccccggggat aacaggctta tctcccccaa gagtccacat 2940 cgacggggag gtttggcacc tcgatgtcgg ctcgtcgcat cctggggctg tagtcggtcc 3000 caagggttgg gctgttcgcc cattaaagcg gcacgcgagc tgggttcaga acgtcgtgag 3060 acagttcggt ccctatccgt cgcgggcgca ggaaatttga gaggagctgt ccttagtacg 3120 agaggaccgg gatggacaca ccgctggtgt accagttgtt ccgccaggag catcgctggt 3180 agctatgtgg gcagggaaaa 3200 <210> 9 <211> 1830 <212> DNA <213> Klebsiella oxytoca <220> <221> rRNA <222> (1)..(1830) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 9 gaatgttatc acggagacac acggcgggtg ctaacgtccg tcgtgaagag ggaaacaacc 60 cagaccgcca agctaaggtc ccaaagtcat ggttaagtgg gaaacgatgt gggaaggccc 120 agacagccag gatgttggct tagaagcagc catcatttaa agaaagcgta atagctcact 180 ggtcgagtcg gcctgcgcgg aagatgtaac ggggctaaac catgcaccga agctgcggca 240 gcgacactat gtgttgttgg gtaggggagc gttctgtaag ccgttgaagg tggcctgtga 300 gggttgctgg aggtatcaga agtgcgaatg ctgacataag taacgataat gcgggtgaaa 360 aacccgcacg ccggaagacc aagggttcct gtccaacgtt aatcggggca gggtgagtcg 420 acccctaagg cgaggccgaa aggcgtagtc gatgggaaac aggttaatat tcctgtactc 480 ggtgttactg cgaagggggg acggagaagg ctatgttggc cgggcgacgg ttgtcccggt 540 ttaagcatgt aggcggatgt tccaggtaaa tccggaacgt tactaacgct gaggtgtgat 600 gacgaggcac tacggtgctg aagtgacaaa tgccctgctt ccaggaaaag cctctaagca 660 tcaggtaaca tcaaatcgta ccccaaaccg acacaggtgg tcaggtagag aataccaagg 720 cgcttgagag aactcgggtg aaggaactag gcaaaatggt gccgtaactt cgggagaagg 780 cacgctgatg gtaagtgaag tgacttgctc tggagctgaa atcagtcgaa gataccagct 840 ggctgcaact gtttattaaa aacacagcac tgtgcaaaca cgaaagtgga cgtatacggt 900 gtgacgcctg cccggtgccg gaaggttaat tgatggggtt atccgtaagg agaagctctt 960 gagtgaagcc ccggtaaacg gcggccgtaa ctataacggt cctaaggtag cgaaattcct 1020 tgtcgggcaa gttccgacct gcacgaatgg tgtaaccatg gccacgctgt ctccacctga 1080 gacccagtga aatcgaaatc gctgtgaaga tgcagtgtac ccgcggctag acggaaagac 1140 cccgtgaacc tttactgcag cttgacactg aactttgaac ctgtttgtgc aggataggcg 1200 ggagacatag aagcaagagc gccagctcat gtggagtcaa ccttgaaata ccgccctgac 1260 atgtttgaag ttctaactcg atgaagacct caaagaggac agtgtctggt gggaagtttg 1320 actggggcgg tctcctccta aagggtaacg gaggagcacg aaggtctgct gattacggtc 1380 ggacatcgta aggtcagtgc aatggcataa gcaggcttga ctgcgagagc aacaactcga 1440 gcaggtgcga aagcaggtca tagtgatccg gtggttctga atggaaaggc catcgctcaa 1500 cggataaaag gtactctggg gataacaggc tgataccgcc caagagttca tatcgacggc 1560 ggtgtttggc acctcgatgt cggctcatca catcctgggg ctgaagttgg tcccaagggt 1620 atggctgttc gccatttaaa gtggtacgcg agctgggttc aaaacgtcgt gagacagttt 1680 ggtccctatc taccgcgggc gtaggataat tgagagggat tgctcctagt acgagaggac 1740 cggagtgaac gaaccgctgg tttacgggtt gtcatgccaa tggcacggcc cggcagccaa 1800 gttcggaact ggataaccgc tgaatttttt 1830 <210> 10 <211> 3618 <212> DNA <213> Ochrobactrum anthropi <220> <221> rRNA <222> (1)..(3618) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 10 catcatggga gttggtttta cccgaagcgc tgtgctaacc gcaaggaggc aggcgaccac 60 ggtagggtca gcgactgggg tgaagtcgta acaaggtagc cgtaggggaa cctgcggctg 120 gatcacctcc tttctaagga agatcgagaa taggaaagac gcagtcttcg ggctgatgat 180 ccttctccat cttattagaa catagatcgc aggccagtca gcctgacgat cgctttgcag 240 gcgtgccgcc ttcgtttctc tttcttcatt gttgattgac acttgtaccg ctcacgagcc 300 gtattgcagc tgcgctgctt ggctctgcgc gagcgcgccg catgagcggc gacggactag 360 cgtcctgtat ttggttctaa cggtttgttt gttggttctg atacaagggc ttgtagctca 420 gttggttaga gcacacgctt gataagcgtg gggtccggag gttcaagtcc tcccaggccc 480 accaaattgt gataaggggc catagctcag ctgggagagc acctgctttg caagcagggg 540 gtcgtcggtt cgatcccgtc tggctccacc atcacttttt tggtgtcgag taggacggat 600 agacagtcag tcaacaagag aaagaaccaa gtttgcggac tttacgaagt ctgcgtgttt 660 ctgtatgaaa tcgtgaaaga agatgtaatc ggatcaacta tccagttgat gtcgcaatgg 720 tttgctcaaa ccttgcatta tgattggacg ctaaccgcgc caccgattgt atctcgagaa 780 gctggtcttt ctgctgatat gatcagagct taatgctttg atggatattg gcaatgagag 840 tgatcaagtg tcttaagggc atttggtgga tgccttggca tgcacaggcg atgaaggacg 900 tgatacgctg cgataagcgt cggggaggtg cgaataccct ttgatccgac gatttccgaa 960 tggggcaacc caccttagat agctagaaaa tcaatttagt tggagcaacg ctgttgggtt 1020 taggcccata cagaccgcta ggtcgtcggc ccatgtgggc cgcccccgcg gagcgccagc 1080 atcgtaagat gcgtacggcg cgtgagcgag aactaaatta atttctagtt atcgtaataa 1140 ggtatctaca cctgaataca tagggtgtta gaagcgaacc tggggaactg aaacatctaa 1200 gtacccagag gaaaggacat caaacgagac tccgctagta gtggcgagcg aacgcggacc 1260 aggccagtgg caatagggaa taaagtggaa gaacctggaa aggtttgccg aagtgggtga 1320 tagccccgta cacgtagaac acctgttgtc cttgagtagg gcgggacacg tgaaatcctg 1380 tctgaacatg ggtcgaccac gatccaagcc taagtactcg tgcatgaccg atagcgaacc 1440 agtaccgtga gggaaaggtg aaaagcaccc cgacgagggg agtgaaacag tacctgaaac 1500 cggatgccta caaacagttg gagcccaagg ttcgtcctgg gtgacagcgt accttttgta 1560 taatgggtca gcgacttagt gtatcgagca agcttaagcc ggtaggtgta ggcgcagcga 1620 aagcgagtct gaacagggcg ttcagttcga tgcattagac ccgaaaccaa gtgatctagc 1680 catgagcagg ttgaaggtac ggtaacacgt actggaggac cgaacccata tctgttgcaa 1740 tagatcggga tgacttgtgg ctaggggtga aaggccaatc aaacttggag atagctggtt 1800 ctccgcgaaa tctatttagg tagagcgtcc agcgaatacc cccgggggta gagcactgaa 1860 tgggctatgg ggactcaccg tcttactgat cctaatcaaa cttcgaatac catacagttg 1920 gaagcagagg acagacggtg ggtgctaacg ttcatcgtca agagggaaac aacccggacc 1980 gccagctaag gtccccaagt tctagttaag tgggaaacga tgtgggaagg catagacagc 2040 caggatgttg gcttagaagc agccatcatt taaagaaagc gtaacagctc actggtctaa 2100 ataagggtct ttgcgccgaa aatgtaccgg ggctaaagcc atacaccgaa gctgtggatg 2160 cacatttgtg cgtggtagcg gagcgttccg taagcctgtg aagggacagt cgtgagacat 2220 cctggaggta tcggaagtga gaatgctgac atgagtaacg ataaagggag tgagagactc 2280 cctcgccgaa agtccaaggg ttcctgctta aagttaatct gagcagggtt agccggcccc 2340 taaggcgagg ccgaaaggcg tagtcgatgg gaaccacgtt aatattcgtg ggcctgcagg 2400 tagtgacgga tcgcgtgtgt tgtaaggtct tattggattg atcttgcagc gaagcggttc 2460 caggaaatag ctcctgcata tagaccgtac cctaaaccga cactggtgga ctggtagaga 2520 ataccaaggc gcttgagaga actgcgttga aggaactcgg caaaatgcac gcgtaacttc 2580 ggaagaagcg tgacctccat ttaggcaact aggtgggggt ggcacagacc agggggtagc 2640 gactgtttac caaaaacaca gggctctgcg aagtcgcaag acgacgtata gggtctgacg 2700 cctgcccggt gctggaaggt taagaggaga tgtgcaagca ttgaattgaa gccccagtaa 2760 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga 2820 cctgcacgaa tggcgtaacg acttcccccg ctgtctccaa cgcagactca gtgaaattga 2880 attcccccgt gaagatgcgg ggttcctgcg gttagacgga aagaccccgt gcacctttac 2940 tatagcttta cactggcatt cgtgtcggca tgtgtaggat aggtggtaga ctttgaagca 3000 gtggcgccag ccattgtgga gtcatccttg aaataccacc cttgcctata tggatgtcta 3060 actgcggccc gttatccggg tccaggaccg tgtatggtgg gtagtttgac tggggcggtc 3120 gcctcctaaa gagtaacgga ggcgcgcgat ggtaggctca gaacggtcgg aaatcgttcg 3180 tcgagtgcaa tggcataagc ctgcctgact gcaagactga caagtcgagc agagacgaaa 3240 gtcggtcata gtgatccggt ggtcccgcgt ggaagggcca tcgctcaacg gataaaaggt 3300 acgccgggga taacaggctg atgaccccca agagtccata tcgacggggt tgtttggcac 3360 ctcgatgtcg actcatcgca tcctggggct ggagcaggtc ccaagggtat ggctgttcgc 3420 catttaaagc ggtacgtgag ttgggttcag aacgtcgtga gacagttcgg tccctatctg 3480 ccgtgggtgt aggaatattg ataggatctg atccctagta cgagaggacc gggttggaca 3540 gtctcttctg gtggacctgt ggcctgccac gcagctgggt agcttatacg gacgggataa 3600 ccgctgaaag catcttaa 3618 <210> 11 <211> 2996 <212> DNA <213> Peptostreptococcus prevotii <220> <221> rRNA <222> (1)..(2996) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 11 ctctcatgga gttggcaata cccgagcctg tgagcgaacc ttttagggcg cagcagtcga 60 aggtagggtc agtaactggg gtgaagtcgt aacaaggtag ccgtatcgga aggtgcggct 120 ggatcacctc ctttctaagg atgagaagct gatacgtcag cttctcacgt gagattttta 180 tctcttagaa ataaaatctc aaagtaactt tggattctgt ataatagttc aaaatagaac 240 caacaaaact aaatagcaaa aaatatttcc agtcaagaaa gaaagggcgc aaggtggatg 300 ccttggcaca tgaaggcgat gaaggacgta agtgaacgaa aactagggag agctcacaaa 360 aagcactgac ccctaggtct ccgaatgggg aaacccggct gtggaagaca cagtcattac 420 taagtgaata catagcttag taaagcaaga ccctgtgaac tgaaacatct aagtaacagg 480 aggaaaagaa agaaaactcg attttccaag tagcggcgag cgaaaagaaa agagcctaat 540 ccattgagga atatctagtt agtcgaatca tctgggaaga tgaaccaaag aaagtgaaag 600 tcttgtagac gaaaactaaa taaaccaggg aggaaagtag caccggacac gaggaatccg 660 ttgtgaagat agggggacca tcccctaagg ctaaatacta acatgtgacc gatagcgaac 720 aagtaccgtg agggaaaggt gaaaagaacc cccgaaaggg ggagtgaaac agaacctgaa 780 acctagtgcc tacaagcaga gagagctcta aagagtgatc tcgtaccttt tgtagaatgg 840 gccagcgagt tatcgtatat agcaaggtta aatcttttaa gagatgaagc caaagcgaaa 900 gcgagtctta ataggggcga atagttagat gcgatagacc cgaaaccggg tgatctatcc 960 atggtcagag tgaaggtgaa gtaaaattca ctggaggctc gaaccgggtc ggtttaaaac 1020 gtatcggatg aactgtggat aggggcaaaa accaaacgaa ctcggatata gctggttctc 1080 ctcgaaatag ctttagggct agcctttgat taagatttaa ggaggtagag cactgaatgg 1140 tctagggcgg cttaccgtac caaaacctat caaactccga atgccaaaaa atcaatcaag 1200 gagtcagact tagggggata agctttaagt cgaaagggaa acagcccaac cgacagctta 1260 aggtcccaaa attggattaa gtggaaaacg atgtgggaag gcatagacag cttaggaggt 1320 tggcttagaa gcagccaccc tttaaagaaa gcgtaatagc tcactagtcg agtcggcctg 1380 cgcggaagat gtaacggggc taaaactatg tgccgaagct gcggatttga cattagtcaa 1440 gtggtagggg agcgttctgt aagccgatga aggtgtattg agaagtatgc tggaggtatc 1500 agaagtgcga atgctgacgt gtaagtaacg ataaaggggg tgagaaaccc cctcgccgca 1560 agactaaggt ttcctgatca acgctaatcg gatcagggtt agtcgggtcc taaggctcag 1620 ccgaacggtg aggccgatgg cagaacaggt taatattcct gtactaccta taagagtgat 1680 gtggagacgg aggagtgaca acgccgcgga ctgacggaat agtccgttaa agggtgtaga 1740 tgttgattat cccaggcaaa tccgggataa gagtcgaacc tgaagtatac caatttcctc 1800 ggaaaacggt aatagtgcgt gtaaacatac tcccaagaaa atccgctaaa cttaatctta 1860 taggtacccg taccgtaaac ggacacacgt agtcgggttg aatatactca ggcgcttgag 1920 tgaatcactt tgttaaggaa ctcggcaaaa tgtccccgta acttcgggag aaggggagcc 1980 agagcgatct ggccacagaa accaggccca agcgactgtt taccaaaaac acaagtttct 2040 gcaaaatcga aagatgaagt ataggagctg acacctgccc ggtgctggaa ggttaagggg 2100 aaggcttagc ataagcgaag gctagaactt aagccccagt aaacggcggc cgtaactata 2160 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggtgtaa 2220 cgatttgggc actgtctcaa caaaggatcc cggtgaaatt gtagtagtcg taaagatgcg 2280 aacttacccc acgctaggac ggaaagaccc cgtggagctt tactgtaggc tgatattgga 2340 ctttgagatt agacgtacag gatagttggg agactttgaa acacgcacgc cagtgtatgt 2400 ggagtcaccc ttgggatacc aaccctctaa tattaaagtt ctaacgatga cccttgaatc 2460 agggcatcgg acattgtcag ttgggcagtt tgactggggc ggtcgcctcc caaaaagtaa 2520 cggaggcgtt caaaggttcg ctcagaatgg acggaaacca ttcgtagagt acaaaggcag 2580 aagcgagctt aactgcaaaa cctacaagtt gcgcagagta gaaatacgga cttagtgatc 2640 cggtggcacc gcatggaagg gccatcgctc aacggataaa agctaccccg gggataacag 2700 gcttatctcc cccaagagtc cacatcgacg gggaggtttg gcacctcgat gtcggctcgt 2760 ctcatcctgg ggctgaagta ggtcccaagg gttgggctgt tcgcccatta aagaggcacg 2820 cgagctgggt tcagaacgtc gtgagacagt tcggtcccta tccagcgtgg gcgtaagaaa 2880 tttgagagga tctgtcccta gtacgagagg accgggatgg acacacctct ggtgtaccag 2940 ttgttccgcc aggagcatag ctgggtagct acgtgtggaa ttgataagcg ctgaac 2996 <210> 12 <211> 2047 <212> DNA <213> Porphyromonas gingivalis <220> <221> rRNA <222> (1)..(2047) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 12 atttatgaaa gttggtaaca cccgaaccgg tggcctaacc gttgtggggg agccgtcgaa 60 ggtgggactg gtgattagga ctaagtcgta acaaggtagc cgtaccggaa ggtgcggctg 120 gatcacctcc tttctaagga gtttttgtga gtggaatgtt ggcgtcctgc ctgtgatggg 180 tggggttgag ggcatgctgt tgggttgtgg ggtatcacat gtgtggtggc ctgtgcggtg 240 ctgtgttgcg tgcgtgtgtg cctggtgtgg tgttcgtgtg gtggttgaga actgtatagt 300 ggatgcgagt atctttattg ttgtatcgtt tgtgcgacgt ggtgttgtgc aaagttgtgt 360 agtgcgatcg gtggatgcct tggcaccaag agccgatgaa ggacgttgtg acctgcgata 420 agccctgggg agttggtgag cgagctgtga tccgggggtg tccgaatggg gaaacctgga 480 atgtccggag tagtgtccgg tggccctgcc ctgaatgtat aggggtgtgg gtggtaacgc 540 ggggaagtga aacatcttag tacccgtagg aagagaaaac aagtgtgatt ccgtgagtag 600 tggcgagcga aagcggagga ggctaaaccg tgtgtgtgtt caagccggca ggtgttgcat 660 gtgcggggtt gtgggggcct tgtggttctg ctgccgcagg attggccagt gagaaatgtt 720 gcgtgaaggt gaagcgtctg ggaaggcgta ccggagtggg tgagagtcct gtaactgtaa 780 gcgtggcact ggtgtggggt tgccccgagt agcgtgggac tcgtggaatt tcgtgtgaat 840 tggcggggcc catcccgtaa ggctaaatac tcccgagaga ccgatagtga accagtaccg 900 tgagggaagg tgaaaaaacc tcgaacagag gactgcaatg accctgaacc cgtctgccta 960 caagcggtag gagcgccatt aaggtgtgac ttgcgtgcct tttgcataat gaacctacga 1020 gttactgttt gtggcaaggt taattgttat aatcaagaca aggagccgaa gcgaaagcga 1080 gtcttaaaag ggcgcccatt tagtcacgag cagtagacgc gaaaccaagt gatctaccct 1140 tggtcaggtt gaaggttagt aacactaact ggaggaccga atcggtagcg ttgaaaagct 1200 ttcgaatgaa ctgagggtag gggtgaaaag gctaatcaaa cttgggagat agctcgtact 1260 ccccgaaatg catttaggta gcgcctcgga cgaataccat agggggtaga gcactgtttc 1320 ggctaggggg tcatcccgac ttaccaaacc gatgcaaact ccgaatacct atgagtacta 1380 tccgggagac agactgcggg tgctaacgtc cgtagtcaag aggaaaacaa tccagaccgc 1440 cagctaaggc cccaaaatca tagttaagtg ggaaacgatg tgggaaggca tagacagctt 1500 aggaggttgg cttagaagca gccacccttt aaagaaagcg taatagctca ctagtcgagt 1560 cggcctgcgc ggaagatgta acggggctaa aactatgtgc cgaagctgcg gatttgacat 1620 tagtcaagtg gtaggggagc gttctgtaag ccgatgaagg tgtattgaga agtatgctgg 1680 aggtatcaga agtgcgaatg ctgacgtgag taacgacaaa acgggtgaaa aacccgttcg 1740 ccgaaagacc aagggttcca gggtcaagtt aatctgccct gggttagtcg gggcctaagg 1800 cgaggccgac aggcgtagtc gatggagaac gggttgatat tcccgtacca gtgaaggacc 1860 gtccatactg atattgtgat gctaaccatg ccgatcacgg tgtcatgaag tttttctttg 1920 tggtgttgtg gggtgtgtgg gacccaagct ttggaggtaa gcgtgttaac aggtgtgacg 1980 cagaaggtag ccaagccacg cggtggttgt cgtggtctaa gcgtgtagga tgactggttg 2040 ttaaatg 2047 <210> 13 <211> 3567 <212> DNA <213> Peptostreptococcus anaerobius <400> 13 agcttgacat ccctcggacc ggtgtttaat cacaccttcc cttcggggct gaggtgacag 60 gtggtgcatg gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc 120 gcaacccttg tctttagttg ccagcattca gttgggcact ctagagagac tgccagggat 180 aacctggagg aaggtgggga tgacgtcaaa tcatcatgcc ccttatgctt agggctacac 240 acgtgctaca atgggtggta cagagggttg ccaaaccgtg aggtggagct aatcccttaa 300 agccattctc agttcggatt gtaggctgaa actcgcctac atgaagctgg agttactagt 360 aatcgcagat cagaatgctg cggtgaatgc gttcccgggt cttgtacaca ccgcccgtca 420 caccatggga gtcggaaaca cccgaagccg attatccaac cgcaaggagg aagtcgtcga 480 aggtggcgtc gataactggg gtgaagtcgt aacaaggtag ccgtatcgga aggtgcggct 540 ggatcacctc ctttctaagg agaattacct gctgttcgat tttgaaagtt catactttca 600 aaatttgtac cttgaaaact gaataattta gtgattacaa aagacatcta tcaagttaaa 660 cttgataaaa tatatgagag aaaactcatt aaaaaacacc aattattctt ttaacactgg 720 tcgaaagacc aaaattagaa aaatctcatt aataactggt caagttatta agggtgcagg 780 gcggatgcct tggcactagg agccgatgaa agacgtgata agctgcgata agcttgacat 840 ccctcggacc ggtgtttaat cacaccttcc cttcggggct gaggtgacag gtggtgcatg 900 gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc gcaacccttg 960 tctttagttg ccagcattca gttgggcact ctagagagac tgccagggat aacctggagg 1020 aaggtgggga tgacgtcaaa tcatcatgcc ccttatgctt agggctacac acgtgctaca 1080 atgggtggta cagagggttg ccaaaccgtg aggtggagct aatcccttaa agccattctc 1140 agttcggatt gtaggctgaa actcgcctac atgaagctgg agttactagt aatcgcagat 1200 cagaatgctg cggtgaatgc gttcccgggt cttgtacaca ccgcccgtca caccatggga 1260 gtcggaaaca cccgaagcct gattatccaa cctkysagga ggmagtcagt cgaaggtagg 1320 cgtcagataa ctggggtgaa gtcgtaacaa ggtagccggy atcggaaggt gcggctggat 1380 cacctccttt ctaaggagtc aaaktacgwt rctgttcgat ttttgccaaa agssmattac 1440 ttasmaaaac tttgtacctt garaactraa gtaaacttta gtagaytaca aargttacat 1500 ggwggtgtag ctcaagttgg gagagcacyt gmcttgmamg cagggggtca tatgagstcg 1560 aaasctcatt masasmcacc aagttattct tttamcannc tggttnttcg atsagaacca 1620 saattaraat taaatagctc atsaatatts cyrgtcaags saksaagggy rcarggtgga 1680 tgccttggca ctakgaagwc gatgaaagga cgtgataagc tgaacgataa actagggtga 1740 gctcacaaaa agcactgacc cctaggtctc cgaatggggc aacccggttg tggaagacac 1800 aatcattact aagtgaatac atagtcttag taaggcgata ccctgcgaac tgaaacatct 1860 aagtagcagg aggaagagaa agaaacatcg attttctaag tagcggcgag cgaaaggaaa 1920 agagcctaac tcattagcga tatcttaaag ttagtcgaat tatttgagaa gataaaccaa 1980 agaaagtgac agtcttgtag acgaaagctt taagatacga gagggaaagt atcaccgggc 2040 acgagtaacc cggtgtgaag atagggggcc catcccctaa ggctaaatac taacatggac 2100 cgnatagnna acaagccatn tgatggatga tggtctaaag ccgtaggaag aaaggtaggc 2160 aaatccgcct ttcacaaatt ctgaaagctg ataggtatcg aaaacataag tagagaagtt 2220 gatgactcca tgttgccaag aaaagtcact atccagacca aagcgcccgt accaaaaccg 2280 acacaggtag ggaggtagag aataccaagg acgcgcggna agaaatcngg ctttgttaag 2340 gaactcggca aaatgtcccc gtaacttcgg gagaagggga gcctgagaga tcaggccaca 2400 gaaaccaggc ccaagcgact gtttaccaaa aacacaagtt tctgcaaaat cgcaagatga 2460 agtataggag ctgacacctg cccggtgctg gaaggttaag gggaaggctt agagcaatcg 2520 aaggctagaa cttaagcccc agtaaacggc ggccgtaact ataacggtcc taagatatat 2580 atntatatan atatntntnt atanatatat atatatatat ctatatagat atatatctat 2640 atatatatat atatatatat atatatatat atatatatat atatatatat atatatatat 2700 atatatatat atatatatat atatatatat atatatatat atatatatat atatatatat 2760 atatatatat atatatatat atatatatat atatatatat atatatatat atatatatat 2820 atatatatat atgatagtag tagtagtngt agtaatagta gtagtagtag tagtagtagt 2880 agtagtagta gtatgantag tagtagtgat tactagtagt agtgagtagt gatcagtagt 2940 gatcagtagt actattagtt agtnagtgat gagtagtgat gagtaattat gagtagtagt 3000 natgatgagt gagtagtcag tgatnagtna tcagtaatca tgagtgatna gtnagtagta 3060 gtgagtagtg agtagtgagt aatnagtagt gagtagtgat nagtagtgag tgagtgagtg 3120 atnagtnatn agtgagtnat gagtgagtna tgagtagtna gtgagtgagt nagtnatgga 3180 tnagtnagtn agtgagtagt gaatnagtta atnagtngtc agtgagtgag tnantcagtc 3240 atnantnagt catgagtgag tgagtgatga gtgactgact catcagtgat gatcgtgang 3300 atgatcatca tannatggtg agtatgatat atgagtacta ctatagtata tgatatatat 3360 accgtatata tatngtatat cgtatnggnt ggtatagtat ngacgctata tcgtgtacgg 3420 accnataccg tgnntggtgc ggtagtgata tatntntgna nactgtaccn tagatngata 3480 gntccgggta gatcgctcca taggtgtacg tgtagatggc ctctagtctg gntcggtagn 3540 cggggttcgg gccccanaan ngtgncn 3567 <210> 14 <211> 3104 <212> DNA <213> Peptostreptococcus magnus <400> 14 cccccgccnc cncnagagtt gataacnccc naagccggtg gcntgnccgc aagnaaggaa 60 gctgtntaag gtgggatnna tgantngggn ngangtggtn cnaaggattc ccnnccccgg 120 naanggggga nggatcacct cctttctang taagaaacgc atgatcgaag atgatgcaca 180 agagaagtga tgtttcgaag atactgttta gttttgagtg atactcaggt atgactcaga 240 gatcattgan aactggataa tagaaaataa attgcgatag aaatagaaaa tgagatcgaa 300 atagtaattg ttattattga ggtcnttcta gaaaagtcga aaactaagaa ttttcatcac 360 taaaaaacat aaatcgaaac ggatggatgg attttagtaa atagttgaaa atagtaatca 420 agcaaacacc aaaataggta aagtaagaaa gagcgtatgg cggatgccta ggcacagaga 480 ggcgaagaag gacgcagcaa acagcgaaat gcgacggtta gcagtaagca tgcaacgatc 540 cgtcgatgtc cgaatggggg aacccacctg gagtagagac caggtatcct gaagtgaata 600 aatagcttca gagaggcgag actcagggaa ctgaaacatc taagtacctg aaggcaagag 660 gaaaataaac gaatgattcc ctaagtagcg gcgagcgaaa ggggaagagc ccaaaccgat 720 cttaggatcg gggttgtagg actgtcggca aagagcaaga aatcattata ggcgaacgga 780 atgggaagtc cggcgaaaca gggtgacagc cccgtagccg aaatagtgaa gaagcacgag 840 acagcacctg agtacggcgg gacacgagng aatcnngctg tacgraaatm canccaggna 900 ccatactggt aaggactaaa tactacctac tgtgnaccgt ataggtgaac acagtaccgt 960 gagggaaagg tgaaaagaac cccgngagng gagtganata gaacctgana ccatatgctt 1020 acaagaagtt acgagcccgt taaagggtga tagcgtgcnt tttgtataat gaaccggcga 1080 gttacnatat ggagcgaggt taagcaggat ntgcggagcc gaancganag cgantcttaa 1140 cagggcgaaa gttgcatgtn ntataccnna aaccnagtga tctatccatg accaggttga 1200 anttggggta aaacccgatg gaggaccnaa ccgncccccg ttgaaacgtt ggcggatgag 1260 ttgtggctag gggtgaaatt ncaatcgaac tcggagatag ctggttctcn ccnaaatagc 1320 ttnagggcta gcgtcgaggt aaagtcgtgt gaaggtacag cacgtgaata tgtgatggcc 1380 ccatctcggg gtacttgaac ataatcaaac tccgaatgtc acaaanatat ncttancant 1440 cngacagcgn gtgatnaagt tcattgtcna anngtaaaca ccnccatacc atcnactnnc 1500 ngtccccaan nntatatact aagtnnaagn agatgtggga gantttcnaa cnaacntncg 1560 nnngtttccc ttcccantcc ntcccatttt ttnnancagt gcnntanccn ccctcatttn 1620 ggtcgnnntg gactctngct ccnantattn ncccgngncc tnagnnnact tacccggaan 1680 tngntggaan ttncccctta annggagnng annanttnnn gggngntnng tnntatnnnn 1740 aaanttatgn attttangng tnagcgtgag tggtagggga gcgttntatg tgcggagaag 1800 gnggtacngt aaggagcngt ggagcgcata gaagagagaa tgccggtgtg agtagcgaaa 1860 cgtgggtgag aatcccacgc accgaaaacc caaggtttcc agaggaaggt tcgtccgctc 1920 tgggtaagtc gggacctaag scgaggccga gaggcgtagt cgatggacaa caggtagaga 1980 ttcctgtact tacggtatga atgatggagt gacggagaag gctagcggat cctgctgatg 2040 gaaatgcagg tgcaagcgag gtagccgaca gccaggcaaa tccggctgtc gaaaggcaaa 2100 ggcgtgaggc gtatggaaag ctgcggcaag tacagaagtc cgtgaagcca gcttccaaga 2160 aaagcttcta gtgataatca tacagtaacc cgtaccgaaa atggacacac atgggtgagg 2220 agagaatcmt aaggtgagcg tagagcaact atagctaagg aactctgcaa aatgactccg 2280 taacttaggg ataaggagtg ctcatagaga tatgagccgc agtgaaacgg cccaagcgac 2340 tgtttaccaa aaacacagct ctatgctaag tcgaaagacg acgtatatgg ggtgacgcct 2400 gcccggtgct ggaaggttaa gaggatgtgt cagcgtaagc gaagcattaa ttaagcccca 2460 gtaaacggcg gccgtaacta taacggtcta agtnananac atgcacncca gtgtgtnagg 2520 agtcaatgtt ggnanannac tnttctngta ttgnagttnt anccgnatgc catgnaantg 2580 gcaacgggac agtgtcaggt gggcagtttg antggggcgg tcgcctccca aagagtaacg 2640 gaggcgccca aagataccct cagcttggat ggaaatcaag cgcagagtgc aaaggcataa 2700 gggtgtttga ctgcgagacc tacaagtcga gcagggacga aagtcgggct tagtgatccg 2760 gcggtgctga atggaaaggc cgtcgctcaa cggataaaag ctaccctggg gataacaggc 2820 tgatctcccc caagagttca catcgacggg gaggtttggc acctcgatgt cggctcatcg 2880 catcctggag ctgaagtcgg ttccaagggt tgggctgttc gcccattaaa gcggtacgcg 2940 agctgggttc agaacgtcgt gagacagttc ggtccctatc tgtcgtgggc gtaggaagtt 3000 tgagaagatc tgtcctcagt acgagaggac cgggatggac atatcaatgg tgcaccagtt 3060 gtcacgccag ggcacagctg gtagnctaaa tangaaggga aaan 3104 <210> 15 <211> 4243 <212> DNA <213> Fusobacterium necrophorum <400> 15 cccnccgtca acaccgacgc anagttggtt gcaccctgna agkagcaggc ctaaccttag 60 ggaaggatgc tccgagggtg tggttagcga ttggggtgaa gtcgtaacaa ggtatccgnt 120 acgggaacgt gcggatggat cacctccttt ctaaggagta ttcttgttgt tctttcttct 180 ttggaagggt tcgcgcatgg accttggaaa ctgtatagta gatcaaaaaa caaacaagaa 240 taaagaacaa agaactctag tttctagagt tagctgscaa agaaaamaac msgdttncaa 300 agtamcaaag ggcacacaag ggatgcctag gtagaaagag ccgaawaagg acgtggtaag 360 ctgcgataag cttggcgaag ttgcaaacga acgtggatgc caagatytcc gaatggagca 420 atctgtaaag agtcatgtct ttacacgaaa gagggaaccg ggtgaactga aacatnctaa 480 gtaatnccgn aggaaaagta aagtaacaac gataccctaa gtagcggcga gcgaacgggg 540 tagagcctaa accgtattca tgtcaaggat gcagccgttg tggatatggg gtagcngggn 600 aanagagara ranaagaact gcaagnctat ttcgcagaac gtaagcaaag gaacaagaaa 660 gatctgggaa aggtctaccg tagaaggtga aagtcctgta ttggtagntt ttnnnncttn 720 nnnnaaanng cgctgntatm tcttctnnnn nncccnnaag ggnngntaat gnnnnnnntt 780 ggnaacacnn gnargaannn nnnnnnnccn nnnttctnnn ngcasraatn ccccntnggw 840 gnantnnnnn nggacnncaa atnctcgtaa ggctanaata ctctctgcga ggtacccaam 900 tagctacgta mggtctmrat agwyggnncs tnasckaacn cgatagtcgc aymgtrccgt 960 gtagggaaag gsgaaaagaa ccccggwagg ggagtgaaag agaacctgaa attgtgtgct 1020 tacaagcggt cagagccact tyggtggtga tggcgtgcct tttggagaat gatcctgcga 1080 gttacgtttc atggcgaggt taagaagaac ggagccgaag ggaaaccgag tctgaatagg 1140 gcgcaagagt cgtggagcgt agacgcgaaa cccggtgatc taagcctgtc caggatgaag 1200 ctgtggtaag acacagtgaa gtagcaggcc taaccttagg gaaggatgct ccgagggtgt 1260 ggttagcgat tggggtgaag tcgtaacaag gtatccgtac gggaacgtgc ggatggatca 1320 cctcctttct aaggagtatt cttgttgttc tttcttcttt ggaagggttc gcgcatggac 1380 cttggaaact gtatagtaga tcaaaaaaca aacaagaata aagaacaaag aactctagtt 1440 tctagagtta gctgacaaag aaacancaag gttaaagtac caaagggcac acaagggatg 1500 cctaggtaga aagagccaac aaggacgtgg tagctgcgat aagcttgcga agttgcaaac 1560 gaacgtggat gccaagatct ccgaatggag caatctgtaa agagtcatgt ctttacacga 1620 aagagggaac cgggtgaact gaaacatcta agtaatccga ggaaaagaaa gtaacaacga 1680 taccctaagt agcggcgagc gaacggggta gagcctaaac cgtattcatg tcaaggatgc 1740 agccgttgtg gatatggggt agcgggaaag agaaagaaag aactgcaagc tatttcgcag 1800 acgtaagcaa aggaacaaga aagatctgga aaggtctacc gtagaaggtg aaagtcctgt 1860 attggtagtt ttcttgcgct gtatctcttc tcccaagtaa tgtggaacac gaggaattct 1920 gcatgaatct gcgaggacca aatctcgtaa ggctaaatac tctggaggtn cctaacccac 1980 cgccgtatga aaagttgggg gatgaggtag gtttankggg tgaaaagcca stckaaccng 2040 ggagatagcc tcgttctctc cgaaatgcat ytacwgtgcm gtccttgcgt tgtnttaatg 2100 atgggggtag mawcactgac tgatactakw ggggcstata tgcttactga anttcaatgc 2160 aaactccnga ataccsttta tntcaagakc gcagtgagtn gagacccatg gtgnagttma 2220 cttnccatcg tnnccgasat ggtcgaaacc amtttttccc agaccaccag mnnnttnaag 2280 ggatcccssa ttcsgtatct tamgtggtgc aaaagggagg ttgtgcakat tnctntaaac 2340 aantnnaggc agnaaaanng tgttngnnng cntgnattnt ntantgntnt nanttngttt 2400 nnnnacntnn nngtttnttt ttnntntttn tccnntantc ntnntctccc tcnncntnnt 2460 nnntnagttn ntttattatt tgatgncnng attctnnttt gtntntttct nttngnnntt 2520 nnttccnncc nnccccttcg ttnnatcgnc gtaaggtccc nnatcatatn taagntnnnt 2580 tgnggaaagn aggtggagmt tcttaanaca acntaggagg ttggcttaga agcagccatt 2640 ccttgaaaga gtgcgtaaat agctcactag tcgagagtct ctgcgccgac aatgtaacgg 2700 gngctaagat atgaaccgaa gctgtggatg tcgtaagaca tggtaggaga gcgttctgta 2760 ggccgtcgaa ggaggactga aaggaactct ggaggtatca gaagtgagaa tgcaggaata 2820 agtagcgasa aggggagtga gaatctcccc cgctggaaga ccaaggtttt cagggtaaag 2880 cttgtcttcc ctgagtaagc cgggacctaa gcccaggcta gaatgcgtag gcgtaatgga 2940 aaacagnats aatatttact gtgccagttc ctagctttgt gaaggtaggg aacgccagaa 3000 gggtatgcgc gcagacgaac ggaagagtct gtagaagcat gtagagtgac ttggtaggca 3060 aatccgccag gttagacttg aggtgtgaca tatactcgca agaggaatgc gcaaatccca 3120 cgctgccgag aaaagcttct agcggtaaag tagagactgc ccgtacttgg ataaccgacc 3180 cacaggtggt caggatgaga aatayttaaa gcggtmcmgg gctracwywc gttaaaggaa 3240 ctctgccaaa atggccccgt aacttccggg aagaaggggt gcctcttggt gtgagtatac 3300 aagcnataca aagcgcanag aggtcgcagt gaagaggctc aagcaactgt ttawcaaaaa 3360 cacaggtcta tgcgaagctg taaggcgaag tatatgggct gacacctgcc cagtgccgga 3420 aggttaanka ggaggtagtg agartctacc gcaattggaa ggccccggtg aacggcggca 3480 cgtaactata acggtcstaa ggtagcgaaa ttccttgtcg ggtaagttcc gacctgcacg 3540 aatggtgtaa tgatttgagc gctgtcttga cgggaggcct ggtgmmattg tattaccggt 3600 gaagataccg gttacctaca gtaggacgga aagaccccat gsagctttac tgtagcttgg 3660 tattgggttt tggcatggca tgtataggat agttgggaga ctgggaaggt atggcgctag 3720 ctgtaccgga gtcatcggtg gaataccaac cattccctgc tgaaattcta atctgtactt 3780 tggaggtatg gagacagtgc taggtgggca gtttgactgg ggcggtcgcc tmacraaaga 3840 gtaacggagg crtwcaaagg ttctactcag gttggatgga aatcaaccgc agagtgcaag 3900 ggcaaaagag agtctkgact gcaagaactg acgggtcgag cmgatgcsaa agcatgacat 3960 ngngatccgg ccatttcgna tggaagggtc ntnnctcaaa aanaaaaann nctnccctgg 4020 agatnacagg ctgatcctac ccgaaaattc atatggacgg gnnggtttcn cancttgatg 4080 tcngctcatc cnatgntggg gnaagaaaaa annncccaag ggntgggctn nnccnnnnaa 4140 naaannngna ncntnanctn ggaannaaaa cnnnaaaaaa aannnnccgn tccnatnnag 4200 ngggnngnnn nannaatnnn nnnnannnnc tntncnnaaa nan 4243 <210> 16 <211> 2625 <212> DNA <213> Proteus vulgaris <400> 16 nncatgggta gtgggttgca aaataagtag gtagcttaac cttcgggagg gcgcttacca 60 ctttgtgatt catgactggg gtgaagtcgt aacaaggkaa cccgtanggg gaacctgcng 120 gttggatcmc cycccttmcc taaragnata cgtgttatgt gcmgkgnnnc tcacacagac 180 ttgtytkatk aagaacgagc aaaangsgsg tytgcgaaag ctgacntgaa gtcccccttc 240 gtytagaggc ctaggmcmcc sccctttcac ggcggtaaca aggggktcga atccccntag 300 gggacgccaa ttgcgcggta tgagtgaaag gsgtcccmcm ctatagtytg atgcmaatca 360 aanaawagtt aagataattt tagcaagtta ttttaactat tatgctyttt taacmatctg 420 gaaccagctg naaaattgaa aaccaatcca tntatccccn aggnataatn atgagtntnt 480 ccaaaatntc aaactttgna tngttttttg ccntcgaagt gggangancg agccatttac 540 ngtttgaggc ggccngngnn cngtnagngc cacnnacntt annangntag nnnggcganc 600 cctgccccac nacnnaangn natttgnncn nccntccccn cccnnacnng tcattaaaaa 660 gaaacatctt cgggttgtga ggttaagcga ataagcgtac acggtggatg cctaggcaat 720 cagaggcgat gaaggacgtg ctaatctgcg ataagcgtcg gtaaggtgat atgaaccgtt 780 atacccgacg atttccgaat ggggaaaccc aatatccaat ggatattatc attaactgaa 840 tacataggtt aatgaagcga accgggagaa ctgaaacatc tcagtacccc gaggaaaaga 900 aatcaaccga gattccccta gtagcggcga gcgaacgggg aacagcccag agtcttaatc 960 aacagcagca tcaggagaac ggtctggaaa gtccggcagt aaagggtgat agccccgtat 1020 ccgaagatgc tgttattgtg aactcgacga gtagggcggg acacgtgttc cmtccttgwc 1080 tagaatatgg ggggaccatc ctgccaaggc taaatactnc ctgawtgacc gnatagtgaa 1140 cccaangtac cgtgagggaa aggcgaaaag aaccccggcg aggggagtga aaaagaacct 1200 gaaaccgtgt acgtacaagc agtaggagcc ccancactaa gctnntggtg aactcacann 1260 nattcttttg catgataaan agcggnagac gacgcnaagt gacgtccaac caatccaatc 1320 aggcagagga ggcttagtgg tggggtgact gcgtaccttt tgtataatgg gtcancgact 1380 tatattctgt agcaaggtta accgaatagg ggagccgtag ggaaaccgag tcttaactgg 1440 gcgaatgagt tgcanggtat acacccgaaa cccggtgatc tatccatggg caggttgaag 1500 gttgggtaac actaactgga ggaccgaacc gactaatgtt gaaaaattac cggatgactt 1560 gtggatgggg gtgaaaggcc aatcaanccg ggagatagct ggttctnccc gaaagctatt 1620 taggtacngc ctcgtgaact catcttcggg ggtagagcac tgtttcgact aggggggtca 1680 tcccgactta ccaactcgat gcaaactgct aataccgaag aatgttatca cngnagacac 1740 acggcgggtg ctaacgttcg tcgtgaagan ggaaacaacc canaccgcca gcttaggtnc 1800 caaagtcatg gttaagtggg aaacnaagtg ggaaggctca gacagccagg atgttggctt 1860 acaaccaccc ttatttnaag gaaagtcnct gngaagatgn agtgnagccn cngcnngacg 1920 gagagacccc gcgaacctnc cntatagctt gacactgaac atngagcctc cactgtgtag 1980 gnataggtgg gagactatga agtgtggacg ccagtctgca tggagtcanc cttgccaata 2040 nnccaccctt taacgtttgg atgttactaa cctaggcccn gtaatcncgg gtncgggtwa 2100 mcgtgtntcg cccgtgggta gtttgactgg ggcggtctcc tcctaaagag taacggagga 2160 gcacgaaggt tggctaagca tggtcggaca tcatgcggtt agtgcaaagg cataagccag 2220 cttgactgtg agagtgacgg ctcgagcagg tacgaaagta ggtcttagtg atccggtggt 2280 tctgaatgga agggccatcg ctcaacggat aaaaggtact ccggggataa caggctgata 2340 ccgcccaaga gttcatatcg acggcggtgt ttggcacctc gatgtcggct catcacatcc 2400 tggggctgaa gtaggtccca agggtatggc tgttcgccat ttaaagtggt acgcgagctg 2460 ggtttagaac gtcgtgagac agttcggtcc ctatctgccg tgggcgttgg aagattgaga 2520 ggggttgctc ctagtacgag aggaccggag tgaacgcacc actggtgttc ggggttgtca 2580 tgccaatggc attgcccgag tagctaagtg cggnaagcag ataac 2625 <210> 17 <211> 4372 <212> DNA <213> Enterobacter aerogenes <400> 17 cccggnaagg ggccccaann ncnngggtna antngnnann nnantnngga agggnnnacn 60 nnanccnagg ggggnnggnc ntnntnnncc ncccnccntt ttaanaaaag gnngaaacnc 120 cnttnncnnn annggngcct gcggnannat nncancgggn cnttananna anccgccnnt 180 cacgccatcn accatgwgta gtgggttgct nangaaanaa ngtaggtaag cttaacmttc 240 twwrcagwgc ngctgtacca ctttcwygat tcatgacttg wwwywanang tcgtracaag 300 gtamccgtag grgaacctgc ggkyggnatc acctcctymc cnttaanaga rccsgcckty 360 gcagygctca cancagattg tctgatgraa gtaanagaag caaggcgtct tgcgattgag 420 mcttcagtgt ccccttcgtc tawaggccca rgacaccagn ccnstntcac wwcggtaacc 480 aggggttcna grtcccctak kgganncgcg crcatbgctc wtcgttmgtg awtgamagac 540 gcttgaccng aacatattct caagaaytcm tcttcgggtg nrcgttggnn agakatttgc 600 tctttaaaaa tctggatcaa gctgamaatt gaaacgacac acagtctsat gtgtgttacg 660 agtcttctca aatttttcgc gtacacggat gaatgtttts ynaygaaaca tcttncgggg 720 ttgtdgaggt taagcgnact amgacgkacm cggtgngatg ccctggcawt cagakgcrat 780 gaakgacgtg ctaanatact gcgaaaagcg tcggtaaggt gatstgaacc agnttacaac 840 cggcgatgtc cccggnaagg ggccccaann ncnngggtna antngnnann nnantnngga 900 agggnnnacn nnanccnagg ggggnnggnc ntnntnnncc ncccnccntt ttaanaaaag 960 gnngaaacnc cnttnncnnn annggngcct gcggnannat nncancgggn cnttananna 1020 anccgccnnt cacgccatcn accatgwgta gtgggttgct nangaaanaa ngtaggtaag 1080 cttaacmttc twwrcagwgc ngctgtacca ctttcwygat tcatgacttg wwwywanang 1140 tcgtracaag gtamccgtag grgaacctgc ggkyggnatc acctcctymc cnttaanaga 1200 rccsgcckty gcagygctca cancagattg tctgatgraa gtaanagaag caaggcgtct 1260 tgcgattgag mcttcagtgt ccccttcgtc tawaggccca rgacaccagn ccnstntcac 1320 wwcggtaacc aggggttcna grtcccctak kgganncgcg crcatbgctc wtcgttmgtg 1380 awtgamagac gcttgaccng aacatattct caagaaytcm tcttcgggtg nrcgttggnn 1440 agakatttgc tctttaaaaa tctggatcaa gctgamaatt gaaacgacac acagtctsat 1500 gtgtgttacg agtcttctca aatttttcgc gtacacggat gaatgtttts ynaygaaaca 1560 tcttncgggg ttgtdgaggt taagcgnact amgacgkacm cggtgngatg ccctggcawt 1620 cagakgcrat gaakgacgtg ctaanatact gcgaaaagcg tcggtaaggt gatstgaacc 1680 agnttacaac cggcgatgtc cgaatwggga aacccagtgc aaattcgtyg cactatygtt 1740 aactgaatac atargttaac waggcgaacc cgggggaact gaaacattct aaktaccccg 1800 aggaaaaraa akcaaatcaa ccwmwattnc ccccagtagc garmrarcga acggggagca 1860 gcnnccnann nnnntgagtn nnnactgaga tcannnnnna gccaanagtt taaataagtt 1920 tngtgtttag tggaacggtc tggaaarttc cgacggtaca gggtratagt ccsgtacmcc 1980 caaaatscmc aggttgtgta actygaaraa gtagggcggg aacacgtggt antcctgtyt 2040 raatatgggg ggaccatcct ccaaggstta aataytcctg actgaccgta tagtgamcca 2100 gtaccngtga ggraaaggcg aaaagaaccc cgggcgaggg gagtgaaaaa gaaccttgaa 2160 accngtgtac gtacaagcag tgggagcacc tttcggggtg tgactgcgta ccttttgtat 2220 aatgggtcag cgacttatat tctgtagcaa ggttaaccgt ataggggagc cgcagggaaa 2280 ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca 2340 tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa 2400 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct 2460 ccccgaaagc tatttaggta gcgcctcgtg aactcatctt cgggggtaga gcactgtttc 2520 ggctaggggg tcatcccgac ttaccaaccc gatgcaaact acsaataccg aakaatgtta 2580 tcacgggaga cacacggsgg gtgctaacgt ccgtckttga ararggaaac aacccaracc 2640 gccagctaag gtcccaaagt catggttaan tgnggaaacg atgtggaagg cacangcanc 2700 nnncngcccn gatgttngct tanaanccnc cntcntttta anaaagggtn atnnctcnct 2760 ggtnnantnc ncctnnncag aanattnang ggnctaaacc ntncnccnna nntgnnnnnn 2820 nnannctttt tctttntnga aagggnnccc ggannntngt anntagttnn ttgatnngtg 2880 ntngtgnttn aganggtngn ggntgccgna aggatnngag anngtannna cnatnncnat 2940 ngtgtggngt tanntntgaa anagaagagt tagcgtacng ngttngcgga gcanagnnga 3000 ntncttagta nntnnnanan agcgnngnag gnagatgnga tggncgnnta gtnnanattt 3060 gnnggngcng tgnntagagn nnngnntntt nggctntnan tnaaanaatn naggnatgtt 3120 agtgtgtgaa nnantgnnan angtantnat aannnnnnaa aganagagtn gnnnntncnn 3180 ctgnggnttt nnttagnggn ctngcannnn aaganttctt tgttaggtng nagnncnnna 3240 tnntnncagg tnnaggggaa ncntannnnn tgtttnccnc ncnnnnancc anaanantcc 3300 nnnntaagnt ttttntcggn gggannannn cncngggtgg gggannacgn cncntcngga 3360 ggnnggnnaa ncccnccccn nccacccaag gnncccccna taatcnttat tttngnngga 3420 aaatgtgnna nggganngnn cacccnagcn annaggttnt ggtntatnaa nnanccaccn 3480 ttnntttnaa aaaaagggta ntanggttcc ttgntgrant nngncctcwc nggaaaaant 3540 gnancggggt taaccnancc accaaagttw cgccaccgan cactatgtgt tgttgggtag 3600 gggagcgttc tgtaagcctg cgaaggnntt nngnanncct gtwagnngcg tnntangnct 3660 nnggaggtat cagtaagtgc gaatgctgac ataagtaacg tataaagcgg gtgaaaagcc 3720 cgctcgccgg aaggaccaag ggttacctgt tccaacgtta akwgggngnc agggtgagtc 3780 gaccmmsaag gccngrggca cgaaaggcgt agtcgatggg aaacaggtta atattcctgt 3840 acttggtgtt actgcgaagg ggggacggag aaggctatgt tagccgggcg acggttgtcc 3900 cggtttaagc atgtaggctg gttatccagg caaatccgga taatcaaggc tgaggtgtga 3960 tgacgaggca ctacggtgct gaagtaacaa atgccctgct tccaggaaaa gcctctaagc 4020 atcaggtaac atcaaatcgt accccaaacc gacacaggtg gtcaggtaga gaataccaag 4080 gcgwttkaga gaactacgga gtgaaggaac taggcaaaat ggtgccgtaa cttcgggaga 4140 aggcacgctg gtgtgtaggt gaagtccctg cggatggagc tgagaccagt cgaagatacc 4200 agctggctgc aactgtttat taaaaacaca gcactgtgca aacacgaaag tggacgtata 4260 cggtgtgacg cctgcccggt gccggaaggt taattgatgg ggttatccgt aaggagaagc 4320 tcttgatcga agccccggta aacggcggcc gtaactataa cggtncctaa ag 4372 <210> 18 <211> 5502 <212> DNA <213> Streptococcus mutans <400> 18 ntntncttan cncccggtnn nnnnntatna tttntntata taaaattgtg nncgaccacg 60 gttttttgta cngtagnnca anantctant antacantcn tatngaanac ccgtgttntn 120 annanncgng aattnncttn ntagaaangn gttntntaaa anaanccnnn ggggtanntt 180 nttntattag nngncccgtg gtaactncaa tnnntncggt aggcngcttt tgtcnnncca 240 atantgntgn gantnggaat antnntntnt tntnnnnngn gnggganntt ttatggggnn 300 caatncnggt ggggtgtttn ntanngggng nnnnagccan ncntttctta ancnnttccc 360 ancntttttn gnggcccncc ccnaacncaa gttggaagag ngggggagtt ttttttgggt 420 gagnntngcc tncanaaaaa aantnnnncc ggtcaagcga ataagcgcac acggtggatg 480 ccttggcggt cagaggcgat gaaggacgtg gcagcctgcg aaaagtatcg gggagctggc 540 aacaagcttt gatccggtaa tgtccgaatg gggaaaccca cccgcttgcg ggtatcctgc 600 agtgaataca tagctgctgg aagcgaacct ggtgaactga aatatctaag taaccagagg 660 aaaagaaatc aaccgagatt ccgtaagtag cgacgagcga acgcggacta gcccttaagc 720 tgatttggtt ctaggaaaac actctggaaa gagtggccat agaaggtgat agccctgtat 780 ctgaaagggc catttcagtg aagacgagta gggcgggcac gtgaaaccct gtctgaacat 840 ggggggacca tccttcaagg ctaaatacta ctgccgaccg atagtgaacc agtaccgtga 900 gggaaaggcg aaaagaaccc cggagagggg agtgaaatag aacctgaacc gtgtgcgtac 960 aagcagtagg agctccgcaa ggagtgactg cgtacctttt gtataatggg tcagcgactt 1020 actgttcgtg gcaagcttaa ccgtataggg gaggcgaagg ggaaaccgag tctgataagg 1080 gcgcatagtc gcgggcagta gacccgaaac cgggtgatct agtcatgccc agggtgaagg 1140 tgcggtaaca cgcactggag gcccgaaccc actcccgttg caaaggtagg ggatgaggtg 1200 tgattaggag tgaaaagcta atcgaacccg gagatagctg gttctcctcg aaagctattt 1260 aggtagcgcc tcatatgtat cctctcgggg gtagagcact gttatggcta gggggtcatc 1320 gcgacttacc aaaccattgc aaactccgaa taccgagacg gactgtatgg gagacacacg 1380 gcgggtgcta acgtccgtcg tgaaaaggga aacaacccag acccacagct aaggtcccaa 1440 attttgtgct aagtggaaaa ccatgtggaa aggcacagac agccaggagg ttggcttaga 1500 agcagccacc ctttaaagaa agcgtaatag ctcactggtc gagtcggtct gcggggaaga 1560 tttaacgggc taagcacaga accgaagctt ggggtgcata ctttgtatgc gcggtagagg 1620 agcgttccgt aagccgttga aggtggattg agaagtctgc tggaggtatc ggaagtgcga 1680 atgctgacat gagtaacgat aatgcgggtg aagaacccgc acgccgaaag cccaaggttt 1740 ccttgcgcaa cgttaatcgg cgcagggtga gtcggcccct aaggcgagga cgaaagtcgt 1800 agtcgatggg aagcaggtca ntntncttan cncccggtnn nnnnntatna tttntntata 1860 taaaattgtg nncgaccacg gttttttgta cngtagnnca anantctant antacantcn 1920 tatngaanac ccgtgttntn annanncgng aattnncttn ntagaaangn gttntntaaa 1980 anaanccnnn ggggtanntt nttntattag nngncccgtg gtaactncaa tnnntncggt 2040 aggcngcttt tgtcnnncca atantgntgn gantnggaat antnntntnt tntnnnnngn 2100 gnggganntt ttatggggnn caatncnggt ggggtgtttn ntanngggng nnnnagccan 2160 ncntttctta ancnnttccc ancntttttn gnggcccncc ccnaacncaa gttggaagag 2220 ngggggagtt ttttttgggt gagnntngcc tncanaaaaa aantnnnncc agccccccca 2280 nnaggnntgc caagcagnaa nggatgnntn ttttcattng gnaannagat naaagnggna 2340 acaagggcnc nntttnancn nnaannantn gacnacnnnn aancangttc tcangagtcg 2400 gggctttacg tcatngcggg tgtntcnaga atganaggca ncatngtaca attgtcataa 2460 nagtntnctc gntgngacaa caggntatan tcnnccgcca ctttttcgta cngnngggng 2520 gagntnngnc annganatat cgnnnncctt taggaccgtt atagtgtacg gcncgccgyt 2580 sactggttgg cttcaatgta ncnngcayct ataagccgnc ttacgcgtag agcatgctcc 2640 tcttaacckt cwagacamcr gktcaggtcg tctagcccct natacgttcn gccttacrkc 2700 ttytgcagag acnctcgtgt attttgyata aacawtcgcm tgggcctatt caytgcggmt 2760 ctmgtcgggc ygtgcaccct agnayagagc aymcctgtct cccgnancaa aaatgncnnt 2820 tnacggggtc attttgccga gttccttaac gagtatgttc tctncgatnc accttaggta 2880 ttctctcctn cgcctacctg tgtcggtttg csgtacgggg cacctctcac ctcgctagag 2940 gcttttcttg gsagtgtgga atcaggaact tcgctactat atttcgctcg ccatsacagc 3000 tcacngcctt acgggaaacg grtttgccta tttmccagcc taactgcttg gacgcggata 3060 tccaataccg cgcttgccct atcctcctgc gtccccccat tgctcaaatg gtganggagg 3120 tggtacagga atatyracct gytgtcccat cgmctacgmc tttcgksctc gscttaggks 3180 ccgactmacc ctgmgcsgay kaacstttsc kcaaggaaaa ccttrggcwt tcngngcgwg 3240 cgggwtyytc anncccstca ttatcgytac tcatrycrgc attckcactt cygatagcgc 3300 tccagcgagw ccttctcrrt ctcrccttca acrgccctta cggaacgctc ctcctaccac 3360 tggttcgcat aasaacagtt atgcacscca agccttctgt gwtacygtkc ttaggccccc 3420 ggttamatyt tycsgcgcas accgtnacct cgrcccagtg agctattacg ctyctcnttt 3480 aaanggngtg gctgcttcta agaccaacmt ccctggytgt ctgtaaagca actttccaca 3540 atrstttttc cactttagca caggaatact ttngggacct twgccttgct gggtctgggg 3600 cttktttccc ttttmackam gggatckttw gcacctcggc cgtgctgwcn ntcccaaggg 3660 gtacagatcc ktctcggyan ttyggggagt ttgcaactgg wattcggkaa gyccggcgrk 3720 grgcccccta gycatnnana cagtgctcta cccccgagag gatacatatg aggcgctacc 3780 taaatagctt tcgaggagaa ccagctatct ccgggttcga ttagcttttc actcctaatc 3840 acacctcatc ccctaccttt gcaacgggag tgggttcggg cctccagtgc gtgttaccgc 3900 accttcaccc tgggcatgac tagatcaccc ggtttcgggt ctactgcccg cgactatgcg 3960 cccttatmag actsggtttc cccttcgcct cccctatacg gttaagcttg ccacgaacag 4020 taagtcgytg acccattata caaaaggtac gcagtcactc cttgcggagc tcctactgct 4080 tgtacgcaca cggtttcagg ttctatttca ctcccctctc cggggttctt ttcgcctttc 4140 cctcacggta ctggttcact atcggtcggt cagtagtatt tagccttgra ggatggtccc 4200 cccatgttca gacagggttt cacgtgcccc gccctactcg tcttcactgr aatggccctt 4260 tcagatacag ggctatcacc ttctatggcc rctctttcca gagygttttc ctagaaccaa 4320 atccagctta agggctagtc cgcgttcgct cgtcgctact tacggaatct cggtatcgca 4380 tttgctttct ccttgctccg gktacttaga trtttcagtt caccmggktc tgccttctca 4440 kcakcctatg wattcactgm tatggatact gctcgcatta gcgagcagtg ggtttcccca 4500 ttcggamatc taccggatca aagcttgctt rccagctccc cgatagcwtw tcgcagtgyt 4560 cgtccacgtc cttcatcgsc tcctgasygc caaggcatcc accgtggysc stcttatstm 4620 rncttraccg tcwaaaagga atcactacgt ggatatcctt gcattacaat tgaatgngna 4680 tgtctactnt cgtnntanct agttaacaaa sancncgctw tgnaggaatg mtccttmana 4740 actmaacaag atnccraccg ctcctgntnt atacgaccgs gaggtctant attccgtata 4800 ancntcckwa gagaggaggt gatncmgccs cnccttccga tgcggcttcs ttgctacnac 4860 ttcacccaan natcatctgt nccacnnctk cggcggstgg ctcnanaana ggctacmtca 4920 ccgactgcng gtngcnacna nantnntggn ggnncgacng gngngtncga acaanatnct 4980 tannaaagan gnnatggcgc cgnncttccg ntacagctac cttannncaa nntcacccgn 5040 aannnctgnn ccntncntcn ggnnacnngg cttccaaaaa ngggtacnnc aacngannnn 5100 ggnnagcgnc nnanntncna cgggggggnn nnnatccngn gacggngcnn atacncatnc 5160 nnaaanannn aannncaccg ntangnnntn anngnagngn nnnnnnngat agtncncacn 5220 ncgananann annncnanac ccccnnnnca antatannnt nnacncannn aaaccnaann 5280 aantnaagnt ttnacncgnn nnnaannnna ccnccnnnnn ncgcngcaan nacnannann 5340 ncnnaagnnn annnnngaac nnnttngang tannangtnt nccnnnncca ngngnntgng 5400 ncatnnngcn ncnnnggcnn gagnnggngn nnnnncactn ncnactntta nngnngnngt 5460 aanttnnntc annnnnntan nngggncttn annnnnnacg cn 5502 <210> 19 <211> 4488 <212> DNA <213> Kingella kingae <400> 19 gggcngganc cccccccttn ttannnaaaa gaagagatng ttagggtncn cccccntatc 60 ggnnanttag atgaagatgc gaagagcnaa naagnacnca anangggttt tgtagctcag 120 gtggttagag cacacgcttg ataagcgtgg ggtngtaggt tcaagtccta ccagacccnc 180 caggattaga gaagcgaatt caagttgtaa agcttacgag aagtaagaaa tactgggggc 240 atagctcagt tggtagagca cctgctttgc aagcaggggg tncatcggtt cgatcccgtt 300 tgcctccacc aagattaaat aaaatgaaaa attagattgc aaattaaagc aagtttagat 360 aaactggcga gcttacttta atttgcgata tattttttat aagcgaaagc tgagaagaag 420 tataattaaa cgcatcgatc tttaacaaat tggaaagccg aaatcaacaa acaaagacaa 480 tgttgtcgat ttggtttggg attgctnngn atnnnnangc aacagtattt caggcagcct 540 gcaaaggcaa caaatcgaat aacaaaattt gggtgatgat tgtatcaact aatcttgaat 600 tcaaaaggca aggttagtac acaacaagca gtaagcttta tcaaaagtag agaatctaag 660 ttattttagt agtcaacgct aaaatagcaa agtcagaagg ttcttcaaat wataggagtn 720 caagtwaata agtgcaycat gtactttgat tcaatgcgat gtggggacgg agaaggttag 780 gttagcaaac tgttggaata gtttgtttaa gccagtaggt ggaaagagta ggcaaatccg 840 ctctttctta acaccgagac gtgatgacga gtgtctacgg acatgaagtg accaatacca 900 cgcttccagg aaaagccact aagcttcagt tgaatcagaa ccgggtggat gccttggcga 960 tgataggcga agaaggacgt gtaagcctgc gaaaagcacg gagaagctgg caaaaaagca 1020 atgataccgt gatgtccgaa tggggaaacc cactgcactc gtgcagtatc ctagtctgaa 1080 tacatagggc tagagaagcg aacctggaga actgaaccat ctaagtaccc agaggaaaag 1140 aaatcaaccg agattccgca agtagtggcg agcgaacgcg gaaaagcctg tatatgataa 1200 tggttaaaga tagaaagaag ggattggaaa ncttccgcca tagtgggtga tagccccgta 1260 tttgaaatty ttaaccatgg tactaagtat acgagaagta gggcgggaca cgtgaaatcc 1320 tgtctgaata tggggggacc atcctnccaa ggctaaatac tcatcatcgn accgnatagg 1380 tgaacccagt accgtgaggg aaaggcgaaa agatnacccc cggaagggga gtgaaataga 1440 acctgaaacc tgatgcatac atacagtggg agcatctttg tggtgtgact gcgtaccttt 1500 tgtataatgg gtcaacgact tacattcagt agcgagctta accgagtagg ggaggcgtag 1560 ggaaaccgag tcttaatagg ngcgactagt tgctgggtgt agacccgaaa ccgagtgatc 1620 tatccatggc caggatgaag gtgcggtaaa acgcattgga ggtccgaacc cacgcatgtt 1680 gcaaaatgcg gggatgagct gtggataggg gtgaaaggct aaacaaactc ggagatnagc 1740 tggttctccc cgaaaactat ttaggtagtg cctcgagtat gagactgatg ggggtaaagc 1800 actgttatgg ctagggggtt attgcaactt accaacccat ggcaaactaa gaataccatc 1860 aagttgctcc tcgggagaca gacatcgggt gctaacgtcc ggtgtcaaga gggaaacaac 1920 ccagaccgcc agctaaggtc ccaaatgata gattaagtgg taaacgaagt gggaaggcac 1980 agacagccag gatgttggct tagaagcagc catcatttaa agaaagcgta atagctcact 2040 ggtcgagtct tcctgcgcgg aagatgtaac ggggctcaaa tctataaccg aagctgcgga 2100 tatacttttg tatatggtag gggagcgttc tgtaagcctg tgaaggtgac ttgtaaaggt 2160 tgctggaggt atcagaagtg cgaatgttga catgagtagc gataaagtgg gtgaaaagcc 2220 cgctcgccga aagcccaagg tttcctacgc aacgttcatc ggcgtagggt gagtcggccc 2280 ctaaggcgag gcagaaatgc gtagtcgatg gaaacaggtt aatattccnt actttattca 2340 atgcgatggg tgtnanttnt tannggnnnn tgtgttnnta nannnnatnn nttngggtnn 2400 nannnnnagt taatgntnga ttantgggag tgntatntan gnaaattann ntttgnnnag 2460 atgnannnna tnnncgtgnn ntannaacng gtcaanttnn ngangcangt nanantaatg 2520 agnnaggnaa nngnnatagn gcnngnagaa gcgggnggtn nnnnnntnna nnggaananc 2580 nngggnttan cgtngtgaag tggtggnngt tgntnntntt nnantattnn gtaatgngnn 2640 annngganna ggngngtnga ngngnntann atntnanaaa aagaananan angnnggtan 2700 tagnnatngg ntgnaattan tnnnagggng gatngtnngt nnattgntgg ttngtanatn 2760 tntnnagnnn ttatggnagg angntganaa gattnnaaan anntnaaagt nttntgatnt 2820 gnatnggngt nngtnnnnnt ganntttann gtnaggtagn nantntngga nggnnngnng 2880 ggtnnaaggt tangnangnn ngttgttnnn annnnggggg gnggntggna gnnangttta 2940 atttttantn gnntnatgtt gggggnggtg ngngngnggn nnnanntagg ggnaagggcc 3000 caagtanagt cantatgngt naaggagcag ngttaatatt cctgtacttn tgattcaatg 3060 csatgtgggg acggagaagg ttaggttagc naaactgttg gaatagtttg tttaagccag 3120 taggtggaaa gagtaggcaa atccgctctt tcttaacacc gagacgtgat gacgagtgtc 3180 tacggacatg aagtgaccaa taccacgctt ccaggaaaag ccactaagct tcagttgaat 3240 cagaaccgta ccgcaaaccg acacaggtgg gcaggatgag aattactaag gsgsttgaga 3300 gaaytmggga gaaggaactc ggcaaattga taccgtaact tcgggagaag gtatgccctc 3360 taatgttaag gacttgctcc gtaagcatct gagggtcgca gagaatcgna gtgngctgcg 3420 actgtttatt rwaaacacar cactctgcta acacgaaagn tggacgtata gggtgtgacg 3480 cctgcccggt gctggaaggt trattgaaga trtgcncaag catcgratmg aagcctccag 3540 taaacggcgg ccgtanasta taacggtnct naagggangg nnatncctnn ttnggtaant 3600 tcggaccccg cangaanggc gtaacatatg gccacncatg tntcctcccg agactcagcg 3660 aagntgaaat nnttgtgaan atgcaatact cnccantgnn ggttagaaag acgccgtnan 3720 cctttactgt agcnnngcan tggacntngn agtcacntgn gtngtatagn ngggaggntt 3780 tnaagcagac gacgtccagt cnctgtggag accgtccttn aacanaccat cccnggcgaa 3840 gttncaggtn gctaacccag acccnttacc cggntccggg gncccnagca accagtntnn 3900 nngnancngt ccttnaannc cncccnngnn nantttaggt tnnaacccag acccgttatc 3960 cngggtcggg gaccgtgcat ggtaggcagt ttgactgggn cggtctcctc ccaaagagta 4020 acggaggagt tcgaaggtta cntaggtccg gtcggaaatc ggactgatag tgcaatggca 4080 aaaggtagct taactgcgag accgacaagt cgagcaggtg cgaaagcagg acatagtgat 4140 ccggtggttc tgaatggaag ggccatcgct caacggataa aaggtactcc ggggataaca 4200 ggctgattcc gcccaagagt ccatatcgac ggcggagttt ggcacctcga tgtcggctca 4260 tcacatcctg gggctgtagt cggtcccaag ggtatggctg ttcgccattt aaagtggtac 4320 gtgagctggg tttaaaacgt cgtgagacag tttggtccct atctgcagtg ggcgttggaa 4380 gtttgaaggg ggctgctcct agtacgagag gaccggagtg gacgaacctc tggtgtaccg 4440 gttgtttcgc cagaagcata gccgggtagc taagttcgga agagaaaa 4488 <210> 20 <211> 3177 <212> DNA <213> Bacteroides ovatus <400> 20 atnnngnggn nnntnnnnnn nnnnnangcc cccnnncnaa ncncnnnaaa aaacncnggg 60 yccctraagg tacgttaccs carraaagck tcctangggt aaaactggta attggggsta 120 aagtsgtaac caaggwagcc ggtmccggaa aggtgcggct kgtaamaggn ccyccntttc 180 tggaagcgna atgccggtaa ctgtcmcaaa aacgtggnca ngccacctaa aaagmcttta 240 aanggtgtcn tgttttttnt gtactanctg gtacttgttt rtttatnana catatagatc 300 amccatctat tagaagtata gatagagata amcaagagaa aaagaagccg agtctagacg 360 aaaggtagac aaggttgaac tagtcctata gctcagttgg ttagagcgct acmctgataa 420 tgtagaggtc ggcagttcaa ctctgcctgg gactaccaag ctcaagaatg aggaacagcg 480 aaagctaaaa gctaaaaaac aaaattcttk attaacctcy ctggggngat tagctcakct 540 ggctaragca tctgccttcg canncgcaga ggngkcaacg gttssaatcm gtnntrttct 600 ccacgatctc tknaaaanag agaaacsact cttctkwcat gatgtacraa aagtagaaat 660 tttagtaaga gctaraagta tatatcaamc cgymcgtgtt cgagtatgca atattgatag 720 aaggaagcat tccwctacca ayaccagtas gcagaaataa aacgtttgwa agtaaagtaa 780 gcaagggcgc atggcggatg ccttggctst cgkaggcgat gaaggacgtg ataagctgcg 840 ataagcttcg ggcaggtgca aataaccttt gatccgaaga tttcygaatg ggacaacccg 900 gcattctgaa ggaatgtcat ccatcctaga tggaagctaa cgcagggaac tgaaacatct 960 tagtacctgt aggaaaagta aaataataan ntgattccct ngcnngtagt ggcgagcgaa 1020 cggggwttag cccaanccaa tgttgttacg gcaatattgg ggttgtagga ccacgatgtc 1080 gcaagaaatt tagtgagaag aaccctttgg aaaatgggac catagacggt gatagtccgg 1140 tattcgaagc taaatgaagc gtagtggtat cctgagtagc gcgggacacg aggaatcttg 1200 cgtgaatctg ccgggaccat ccggtaaggc taaatactcc cgakasaccg atagcgaacc 1260 antactgtga aggaaaggtg aaaagtcact tnccgnaata gcaagcagtg aaatagtccc 1320 tgaaaccgtg cgcctacaag ncggtcggag ctgtgtaaac agtgacggcg tgccttttgc 1380 ataatgaacc tacgagttac tttttccggc aaggttaagg atcttgagat ccgcagccga 1440 agcgaaagcg agtcttaaca gggcgattag tcggaaggag tagacgcgaa accaagtgat 1500 ctacccttgg tcaggttgaa ggttaggtaa cactaactgg aggaccgaac cgataagcgt 1560 tgcaaaagct tccggatgam ctgagggtgg gggtgaaagg ctaatcamac tttgkagaan 1620 agnytggtac tncnccgaaa tgcatttaga gtgcntgncn ttgganaant tnctanctgt 1680 gangnnnnnc nctganannt ncggggcntn ccnnnggnng ggttatatnn ctntnnngcg 1740 agggncntnt anncccnggc nnnngnnnng gnnnnaacnc ccnnntnngg gnaanaaann 1800 ntnntngggg ggtccccnnt wtwttnntan ntncagaana angnatnnat tncntaatan 1860 cannnnngnn gngnttttgg cannnctann cgtcnantnt tncttaaaat nnnaannnan 1920 tnccatanca nnantnntnn tnnttccnnn anaantatta tnntanttac tttnnantat 1980 ttnnncttna ngaatatnat tattcttnan nanttanntt ttantcnntt tttcnnnnnn 2040 ctanatngnn nnannanatt aactcnaatc nnnnanannt aactttaaan atctantaca 2100 tncnannnct anganntatt natanttntn tntgtttant tgnnntnntn ncntcttgnt 2160 ntncnacttt tttantannt ttcntancct ncanntncta nngtnnttan tncnntntcc 2220 nttattannn ttanaangcc aanacatnat cannntctat tnctnaccat catntcttna 2280 tcnnactnac tnctgnnttn atctaccatn cccgaattag gtacccaggc aatgcacctg 2340 gcggcacaac tggtaaacca gcggtcagtc caacacggtc ctctcgtact agtgtcagag 2400 ccacgcaaat ttcatacgcc cacgatagat agagaccgaa ctgtctcacg acgttctgaa 2460 cccagctcgc gtgccacttt aatgggcgaa cagcccaacc cttgggacct tctccagccc 2520 caggatgtga cgagccgaca tcgaggtgcc aaacccctcc gtcgatatga gctcttggga 2580 gggatcagcc tgtntatccc cggagtacct tttatccttt gagcgatgtc ccttccatac 2640 ggaaacaccg gatcanntat gctctagttt cctacctgat nnanttgtct gtctcccant 2700 caagcgccct tatgccattn cacntntacg gacggatacc caatcngttc tgnagggnnc 2760 nctttagaan tcctncgtct anacttttgg nggncnttcn tcccatntaa nancnttcca 2820 ccaaaaagnt gtttctatcn tnttacnagt ttggaacttt taantattta naagggntng 2880 nnagtttant ctatcnnnan tcnnnnnaat tctttggnan cctanttttt ttnnaaanan 2940 tttnnggnnt antnntgtan tnttnagtct ncccnaattn tttnctnttt ntaacgttat 3000 antgttnata gggtnntcat nnngntnntt ntgcgngntn tnttntntnn nnngnatttt 3060 nntnnactnt ancnntnncn tnctaccatc tnntcttntt tctntcnagn ntnnttttta 3120 ttctnncacn aaccttcntc tccgntttnt ttctntnttt tncnnctttc ctccact 3177 <210> 21 <211> 2770 <212> DNA <213> Bacteroides thetaiotaomicron <400> 21 ggggggccnn ccnatnnggt ncctttttaa nntccnnaag gccncnantg ttttantncc 60 nngaaanaan nngcattngg gnnaangnnc cnntnccnaa aanaaaagnn tttggnnncn 120 ccngcccgtc gagccctnaa agccgcgggg tactgaaggt aggtaaccgc aaggagngtc 180 ctagggtaaa ttntggnaat tggggcttaa gtygtnntca nanggtagcc gtaccsgaan 240 ggtgsggctg gaacmccctc ctttctggag cgnatgtsgt tacaacgatt tcatrggttt 300 ctattattgt actactggta cttgtttatt tataatatat agatcamcca tctatagaag 360 tatagataga gataamcaag agaaataaag aagccgagtc taacatcagg tagacaaggt 420 tgaactagtc ytatagctca gttggttaga gcgctacact gataatgtag aggtcggcag 480 ttcaactctg cctggganta ctccgaacaa aaattgtcgt tgccaataca gtgttggaat 540 gttcaattac tcttggggga ttagctcagc tggctagagc atctgccttg cacgcagagg 600 gtcaacggtt cgaatccgtt attctccacc atctctgaaa agagaaacga tctttgacat 660 gatgtacaaa aagtaaaatt tagtaaagag ctaaaagtat atatcgaacc gtacgtttta 720 agtacaacac tattaagtac tttgggttaa tacctgatac ttgataccta atacttaaaa 780 gtaagtttga aagaaagtaa gcaagggcgc atggcggatg ccttggctct cggaggcgat 840 gaaggacgtg ataagctgcg ataagctctg ggtaggtgca aataaccttt gatccagaga 900 tttccgaatg ggacaacccg gcattctgaa ggaatgtcat ccatctttga tggaagctaa 960 cgcagggaac tgaaacatct tagtacctgt aggaaaagaa aataataatg attcccctag 1020 tagtggcgag cgaacgggga atagcccaaa ccacccatgt tacggcatgt gtggggttgt 1080 aggaccacga tgtcgcaaga catttgatga gtagaatcct ctggaaagtt gaaccataga 1140 cggtgatagt ccggtatacg aagtcaaatt aagcgtagtg gtatcctgag tagcgcggga 1200 cacgagaaat cttgcgtgaa tnctgccggg accatnccgg taaggctaaa tactncccga 1260 gaagnaccgn atagcgcaca cccaagtact gtgaaggaaa ggnntkwaaa scmctattcg 1320 aatagcaagn agtgataata gtccctgaaa ccgtgccgcc tacaagtcgg tcggagctgc 1380 ttaagcagtg acggcgtgcc ttttgcataa tgaacctacg agttactttt tccggcaagg 1440 ttaagcatct tgagatgtgc agccgaagcg aaagcgagtc tgaacagggc gtcgagtcgg 1500 aaggagtaga cgcgaaacca agtgatctac ccttggtcag gttgaaggtt atrgtaacac 1560 taactggagg accgaaccga tawgcgtatg aaaagcttac cggatgtaac atgakggtgg 1620 gggtgtaaag gmtaatctaw actatrkagt atagtmtmgt actccccgat atatrcatat 1680 atakrtryag ccmtatrtga gtatactata trtgakrtak agckactgat amkatgckag 1740 ggctatcacc gymtatcata gtactntgat atatactccg atatgcgcat ntanttmtat 1800 cacatakagt gatggcatgg rtgmtatata gtccatrtac tatatagkak atakatatnc 1860 ataccntcan ctataggtcc cyagwatawa yastnagtat gnactatact atagwcannt 1920 atatgmtata tacngctnng atrtatagct atggatagya ncctatatat ayatatatak 1980 atrcrtayan ctcactatrt cnnggagtat atggagtgga tactatatat cnggcatata 2040 tantrtatat atacckatat ctatgggatn nnnnatgatn ggnanggtac ntatmccata 2100 tatatatata tatatatata tatatatata tatatatata tatatatata tntncatata 2160 tatctatata tatatatnat atatanctat atatatatat atatatatat atatatatat 2220 agtatatata tatagtanat atattatata tanagtgtat agnctaccat atatatatan 2280 gatagactat atatatntnc ntacanatat atanntatnt ntctananan atgatatnnc 2340 natanngcnn tananannta natatanang tnnatcaggn agtatannag agcatatntg 2400 atccgntgat tccnatanan angtacatng ctcnatagna tatatagnat actccgggna 2460 tatcngtctn tatccctccc atagagntnn atatatcgaa ngggttnggc acntngatgt 2520 cggcncgtcn cancctnggg ctggagaagg tcccaagggt nggnctgttc gcccattaaa 2580 gtggcacgcg agntgggttc agaacgtcgt gagacagttc ggtctctatc tatcgtgggc 2640 gtatgaaatt ngcgtggctc tgacactagt acgagaggta ccgtgttgga ctgaccgctg 2700 gtttaccagt tgtgccgcca ggtgcattgc ctggagtatn ctggagttcg ggtangcgaa 2760 aaaagagnnc 2770 <210> 22 <211> 3455 <212> DNA <213> Clostridium difficile <400> 22 gngnnccnnc nnncnngccn ggggggggnn nccccccncc nncgagncnn tcnnccnncn 60 nncngnggcc nttnttannn gggggggncc cccnngnngg ggnggnnngn annccttcnn 120 cggggggggg ggncagcccc cncntnnnnc cggcagnnng gncccccaaa aantngtnan 180 tgnggngnaa naaggggggg ggggccncac cnaccccccn ggngtttgtt ggtttaaccn 240 cnccccccnc ttccntcaan aggggggcga aagnancccc ngngcnangg ggtccccccc 300 nccgcagaat ttncgngggg gggntaattt cttaccccnn ggagcngnng gcccccagta 360 nanggngggg gggggcggct ccccacgggg tgnngnngca nccccngnnn gcctcangtg 420 cacagtttgg gngngagaaa tggggcncnc gagnagcnna tgntgggggg ngaggncncc 480 cncngccngg acncncnggg ttnccnggnn angntgtccc ccccccntgg ggttagncgg 540 ccccnnaagc tgngcccnaa nggcggtagg tgatggccan cnggtnaata ntccggnacn 600 cccgntanct gtntgcnagr rgnngsagta nntcncnnaa ggnnnagccn tatnagnanc 660 ngnananana tcncnnccng ngttnnnagk rgrayyccnc sctgcgnnca atagcangtg 720 nngatnccnc gcaattnatt tcngttggcg cntngcgtta gcactttgaa aactgcatat 780 atatttagtg atatgacatc taatttgtaa tatataaagc tgataacttt tttaaaatta 840 wcgaangttg atrgcttctn aatctatcaa cnaccntttt taaytggntc aagttattaa 900 gggbgcaggg cggatgcctt ggcactangg arccratgaa ggamgtkant aacgytgcga 960 nntaagcttt cggggagttg camgtwawct ttgatcncga aagatttccg annatgagga 1020 aactcacttn agangtaatg tstaagntat cattaagtra atacatwgct taatgagngg 1080 gaastcaggg aactgawaca tnctaagtac ccgangcaag nanaaagaan tcgnannacc 1140 ganttcnttc ngtaagnang ngngaangan gangcggctt agccccaaac cnaakaaagt 1200 tttgctttat tnnggggykg cnggatcata tcataacgaa gnanggtwtc gtaattgaag 1260 aggtttggww agacccaccm cascaaggta akagtcctgt atgttaaacg agaagacttt 1320 agatatgatc cagagtacca cgggacacgt gaaanccctg tssgaagcag gagggaccac 1380 cctccawggc taaatactac ctagtsaccg aatagccgta tagkaccgtg agggaaaggt 1440 gaaaagaacc ccgggagggg agtgaaatag aacctgaaac cctgcactta caagctgtgg 1500 aagcacattt cttgtgtgac cgcgtncttt ttgtanaacg ggccaacnan ttacnttaag 1560 tagcaaggnt aatcncttaa ggtgcgganc ctnagcgaat ncnatttttt nactgaccgt 1620 tcacttactn gnccnngact cnnnaccggn ccatctnnnc atgnncatag atcnnaatta 1680 nagnnnntcc ccncnctggt cctanctntt atttnaagag tgcgtaatag ctnactggty 1740 ggagtgatcc tgcsccgaag atttccgggg stwtaaactt tactaccgaa agctacgggc 1800 atcagtaatg atgggtaggg gagcttcccn atacgggttg aagcatgacc gtaaggacat 1860 gtggacagta tgggagtgag aatgttggca tgagtagcga gatgtgggtg agaatcccac 1920 aggcccgtaa acccaaggtt tccaggggaa ggttcgtccg cccctgggtt agtcgggacc 1980 taagctgagg ccgaaaggcg taggtgatgg acaacaggtt gatattcctg tactaccgat 2040 aaccgtttga ragaagggat gacacagtag gataagctaa gcacactgtt ggttatgtgt 2100 gcccaagcat tgaggcagtc aaagtaggca aatccgcttt gataatgctg ggatgtgatg 2160 gggagcgaaa tttagtagcg aagtagctga tttcacactg tcaagaaaag tctctatcga 2220 ggttaaaggt mcccgtaccc gcaaaccgac acaggtgggt gaggagaktw tcctaaggcc 2280 agcgagagaa ctgttgttaa ggaactcggc aaaatgaccc cgtaanngnn ncttagggat 2340 aaggggtgcc accatgcagg tggcccgncc agakaantwg gcccwasccg actgtttacc 2400 cnaaaaacnt tnngnttnat nntnnnnnnn nnnnansgnn ntnttncann tnantgcctw 2460 agtncgcaag acgatgtata ggagctgacg cctgcccggt gctggaaggt taaggggatc 2520 tgttakagca atcgaagcag tgaacttaag ccccagtaaa cggcggccgn nactataacg 2580 gtcctaaggt agcgaaattn cttggcgggt aagttccgac ccngccacga aaggcgtaac 2640 gatttggggc acttgtntca acaaacatta ctcggkgaaa attgtaattc ccggtgaaga 2700 tgccggnata ccttgcgaca ggacggaaaa gacccccatg gagctttact gwakgcttga 2760 cattgggtct tggtactaca tgtacakgat aggtgggagg ctttgaaacc casgacgcca 2820 gttttggcgg agccatcctt gggataccac ccttgtagtr ctgggactct aactcatasg 2880 ccatgaatct ggtcttggga cactgtcagg tgggcagtnt atgactggkg cggtcrcctc 2940 ccaaaakgta acggaggcrc tcaaaggtts tctcagtacr rtackgaaat cgtacgtara 3000 gtgtmaaggc ccaaatagrg agctatgrtg tgcmagacat cnancatgtg tgcgagcgan 3060 argatgaaaa tcggacctcc ctrgkgrtcc tggycggtct cttgcgtgga agggnnngcc 3120 rkccgcttcm mcsgataaaa gacwatccyt ggcggataay rggcttttta yacycccccc 3180 nnaagagtcc gaacatnctt grgcnngggg rggcnanaaa tttggncacc atcgatgtcg 3240 gctcatcaca tcctggggct gtagtaggtc ccaagggttg ggctgttcgc ccattaaagt 3300 ggtacgcgag ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcaggcgt 3360 aggaaatttg agaagacctg tccttagtac gagaggaccg ggatggacgt acctctgtgt 3420 accagtgtcc tccaagggca ggcnggagct atncn 3455 <210> 23 <211> 2651 <212> DNA <213> Haemophilus aphrophilus <400> 23 ccngncncnc cannnctggg agtgggttgt cmccgmagya gatagcttaa ccgcaawggg 60 ggcgtttacc mcggtatgat tcatgactgg ggtwmagtcg taacaaggkr accgtagggg 120 aacctgcggt tggrtcmcct ccktaccgan aagacgaacy taagtgtccm cacagtttgw 180 ctgatgattg tagacaagaw agaacaaakc saangngtan nawncnaacn aagntntnna 240 gagcatcttt atatgttgtc cccatcgtct araggcctaw gnacatcgcc ctttcmcggc 300 ggtarccggg gttcgaatcc ccgtggggac rccatataaa gatgattayt catcttcttt 360 ataaaacctg ttctttaaca aaccggaaac avgctgaaaa cgagactttc aagaaagtct 420 gagtgakagt gattgataaa aggtgattac tcttaatama ttaactgtya ctgcttagcr 480 dcdttvvgtg tttayycrat tcaatgttgt gattttaakt ggattytcva tttaatgtta 540 agytcatkat gttgagttan cnatcataag aatacttgag gttgtnatgg ttaagtgaca 600 anagactgtv caattttkgt graatgncnn tcnttkkcaa tcagaggcgt catagttaag 660 gacgtgcncc nnttaatnct gccaagnnan naannnatnn nnnagnctnn nnntnnnngg 720 annnnntgag tncncnnnnn cgncatanag annntnnncg gccccnctgt nnnnttaatc 780 caagatgtcc gaatggggaa acccagtggg tgaagaaccc actatcatta tctgaatcca 840 taggataatg aggcgaaccg ggagaactga aacatctaag taccccgnag caaaagaaat 900 caaccgtaga natctttttt aaannnnctt ttttttnnnn nnccngnnan nnnnnnnnna 960 nnnnnncttn naanaanntt tnnnnngaat gnatnnanaa ggtnantnnt cttnaatnaa 1020 ttnnngntnc nnannancgg ngattanttt ttttnnannn angtngtgat ntttagaggn 1080 tnttncaant nannngtngn tnatgatgag tanntnatna naanaanncn tntggtttnn 1140 ttgnttaang angcagnnna cnaggnngcn tcccntgnca tangagnaga agaangnngt 1200 gatnntntna nanaangttt gantagatna taagaggngt tttnnncaag angncnngan 1260 ggggcncacc nggtggngaa cccnccnnca tnattttnnt cnancntatn atnatgngnn 1320 ccnccaggan taattcatcn nantannccc cngannanaa atcanccnnc nntttnttan 1380 tagnngcgag cnnacganaa gnccgtaggc atcctgttag tgataatgac agsagnacag 1440 aaggcaacaa sctgggtrag cttgnntgcg acacagggca tgakagcacc cgymctcgaa 1500 gtccaggctt akggtastaa gctaacgaca agtaaggcrg gacacgtrat atcctgtttg 1560 aakwtggggg gaccatccyc cmaggctaaa tactacctgn attsaccgat antagnaacc 1620 rgwacctgtg aaggaaaggc gaataagtaa ccmacggtga ggggagtgaa atasaacctg 1680 awaccttgta cgtacaagac agtgggagcc tgaaagggtg actgcgtacc ttttgtataa 1740 tgggtcascg acttatattt tgtagcgagg ttaaccgaat aggggagccg aagggaaacc 1800 gagtcttaac tgggckagta kttgcwaggt atagacccga aacccgktga tctagccatg 1860 ggcaggttga aggttgggta tacactaact ggasgaccga accgactaat gttgaaanna 1920 attagcggat gactcntgtg gctgggggtk aataggccaa ycwaaccggg attkatagcc 1980 tgnttcntcc yygaaattct atktakgtaa anccctttgt ataramamct ktcggggggt 2040 naaacaccct gntntcyggy ttaggggntn cntcatcmcc ktctnttmcn cnataaaann 2100 ncgttntncn atattttgnt cnngaacnan cnntnnttnn tntcntntgn tacanattnn 2160 nnntaacncn cnnttatttt tnttnttnnc tctnntttnt tnnnttnacn tggganacac 2220 nccnganggg tncncnacan ttnnttngtg tgnnnaaggg gaaaccnncc ccactaccng 2280 ntaaanttaa ngtnccnaaa annctttata tntnnantnt tcaanancaa aannattnat 2340 nctnnnnact ntgtttttnn tngttnnacn tnaanannan caaananaca ctntctnnag 2400 antanntnnn ttnntnnnac ttnantcntt ncncccccnn ananacnnnn tananantna 2460 nactnattnt tccnncanat atnnngntac cnnnaaccnc ctnnncnnat tngtanncnn 2520 tnnnntnana tngntnncna tcacntnnnc tnannttnan agagtancga ccaanactnc 2580 ntnctctaca tctnanctct actcnntccc ncccaatacn nncctacnnn ttcttatnaa 2640 cntacncttc n 2651 <210> 24 <211> 3077 <212> DNA <213> Neisseria gonorrhoeae <400> 24 ggntntgngt antgncaaan acnnncctcn agngtgnnnn gnattattat atgtccngnn 60 agaantgaaa cagngngggg nnnngngatn angtanttaa tngntgctnn nggcgngntn 120 gtnnatgnaa agngnttncn gngacancat aactgtnact attnctatnt tgcgcnctan 180 tggtgnagtt tatnngngna gttannnttn gngaagaann tccntnttaa ggannggnaa 240 ttatnnccta tnantngnnt acagtngttt ntnttttccn annttnncgg tnnnnncang 300 nanaaatnnt ntgannnctt attnaaattg gngccnttnn gnnaaannan tncnncngng 360 ttttataana gnnncgttng ttagnagngg ntantctnnc ctannagtnn gcgggntnna 420 ggttccncag acntatnnaa tatcccncta naaccnagng gnnnttgccn nntcgttngn 480 tcannnggnt anannnannc gcttnatnan nnnngggntn gnaggttnna ntccntcccn 540 nnccccncca anannngggg catagntcag tntggtnngn agcaccngct ttgcwagcag 600 ggggtcmtcg agtwcgatnc cgtttgcctc cncccnaaac tttacaaatg aannagcaag 660 tttgctgttt ttagcagctt attttngatt tntgcnngat agctagawta nacgacgcga 720 tmkatctttg ancaaattkg acaagcgcgm watcarcamc acamagacaa tgagtttgtt 780 ttgmtttttt attctttgca aaggataawa acctctcgca agagaaaaga ammgcaamca 840 tagtatttgg gtgatsattg tatcagactt aatcctgart acacaaaagg caggattaag 900 acacaacaaa gcagtaagct ttatcaaagt agggatttca agtttgctta cttagtcaac 960 gggtaggtaa acgcaagtca aagtaagttc ttsaaatsat aggagttcaa gtsaataagt 1020 gcatcaggcg gatgccttgg cgatgatagg cgacgaagga cgtgtaagcc tgcgaaaagc 1080 gcgggggagc tggcaataaa gctatgattc cgcgatgtcc gaatggggaa acccactgca 1140 ttctgtgcag tatcctaagt tgaatacata ggcttagaga agcgaacccg gagaactgaa 1200 ccatnctaag tacccggagg caaaaggaaa tcaaccgcag ntaccgcaan nggggntnna 1260 atnnanancn naanntncgn annccgnttt nccggcatgg tagggnagcg ttntgtagnn 1320 tnannaaggt gcattgtaaa gtgtgntgna ggnatcanaa gtncgaatgt tgacatnagt 1380 anngataaag cgggtnaaaa gcccgctcnc ngaaagccca aggtttcnta ngcaacgttn 1440 atcggngtag ggtaagtcgg cccctaaggc gaggcagaaa tgcgtagtcg atgggaaaca 1500 ggttaatatt cctgtacttg attcaaatgc gatgtgggga cggagaaggt taggttggca 1560 agctgttgga atagcttgtt taagccgggt aggtggaaga cttaggcaaa tccgggtttt 1620 cttaacaccg agaagtgatg acgagtgtct acggacacga agcaaccgat accacgcttc 1680 caggaaaagc cactaagctt cagtttgaat cgaaccgtac cgcaaaccga cacaggtggg 1740 caggatgaga attctaaggc gcttgagaga actcgggaga aggaactcgg caaattgata 1800 ccgtaacttg cgggagaagg tatgccctct aaggttaagg acttgctccg taagaccccg 1860 gagggtcgca gagaataggt ggctgcgact gtttattaaa aacacagcac tctgccaaca 1920 cgaaagtgga cgtatagggt gtgacgcctg cccggtgccg gaaggttaat tgaagatgtg 1980 caagcatcgg atcgaagccc cggtaaacgg cggtccgtaa ctataacggt acctaangta 2040 accaaattcc ttgtctnnta anttccganc ctcnctaatg gcgtaacnat gnccacactg 2100 tgncctaccg atactcaann atagttgaag tggttgttat agatgcaaaa ctatcttgct 2160 tntgnacctg nnagacaaac ntnnaccgnn nactgaagcc tttgcattgg acttggcntt 2220 angagtagcc nngaaanttc ctttttcctg gtaaaaggnn anttcctgaa ccccgccacg 2280 naattgggcg taaccgattn gcccacccct gtcctycctc cctgagactc agcgaaagtt 2340 raagtnggnt tgtgaagatg caattctacc ccgctgccta gacgggaaaa gaccccgtga 2400 accttttack gsagctttgc attgggactt tgaagtcact tgtgtaggat aggtgggagg 2460 cttggaagca gagacgccag tctnctgtgg agtcgttcct tkaaatacca cncctggtgt 2520 cntttgaagg gaatttctaa cccagacccg tcatccgggt cggggaccgt gcatggtagg 2580 cagtttgact ggggcggtct cctcccaaag cgtaacggag gagttcgaag gttacctagg 2640 tccggtcgga aatcggactg atagtgcaat ggcaaaaggt agcttaactg cgagaccgac 2700 aagtcgggca ggtgcgaaag caggacatag tgatccggtg gttctgtatg gaagggccat 2760 cgctcaacgg ataaaaggta ctccggggat aacaggctga ttccgcccaa gagttcatat 2820 cgacggcgga gtttggcacc tcgatgtcgg ctcatcacat cctggggctg tagtcggtcc 2880 caagggtatg gctgttcgcc atttaaagtg gtacgtgagc tgggtttaaa acgtcgtgag 2940 acagtttggt ccctatctgc agtgggcgtt ggaagtttga cgggggctgc tcctagtacg 3000 agaggaccgg agtggacgaa cctctggtgt accggttgta acgccagttg catagccggg 3060 tagctaagtt cggaana 3077 <210> 25 <211> 3540 <212> DNA <213> Eikenella corrodens <400> 25 cgccgtcaca ccatgggagt gggggatacc agaagcaggt agggtamccg caaggagccc 60 gcttgccncg gtatgcttca tgactggggt gaagtcgtaa caaggtagcc gtaaggggaa 120 cctgcggctg gatcacctcc tttytagaga aaaagtaggg gtagagcatc cacacctatc 180 ggtaatcaga tacagatgcg aagagaatgt tgggtttgta gctcagctgg ttagagcaca 240 cgcttgataa gcgtggggtc ggaggttcaa gtcctcccag acccaccagt agtcgtagag 300 cggagcagat ccgcccaggt actgggggca tagctcagtt ggtagagcac ctgctttgca 360 agcagggggt catcggttcg atcccgtttg cctccaccac acaatacttt ccaaataaaa 420 gtatgctaaa tatacgagta agttgctgca tcttgcagtt tgcgcggtag tttattttta 480 tttgcgaagt caaaacgcat cgatctttaa caaattggca agccgaaatc aacaaacaaa 540 cgaagtaggg cttatctttg agataagtca tacagaaatt tgggtgatga ttgtatcgac 600 tgaatcctga aacacaaaag gcaggattcg gacacaacaa gcagtaagct ttatcaaagt 660 agaggcctta agccccagct gtagtcaacg cagcgcaggc gaagtcaaaa aggcacttca 720 aatgatagag tcaagtgaat aagtgcctca ggnggatgcc ttggcnatga tnggcnacna 780 angacntgta ncccngcnaa ngctgnntnt tttnngntng ggggggntat tttttttttt 840 tttnggnaan gacgaagcgn tntttttttn nnnnntnggc ggngctgatn ccccaancna 900 agnagggggg gtnttttttt ttaagtaatc aacanaaaat ttggggattg ttnggannga 960 ttcatcccnc anaanagaaa ggannggttg gnaccaanca gnanggtttt ttttnagtag 1020 aggcgnncnn nnnccccccg tagtnanctc agcncagccg nagtnannaa nnnagntctt 1080 ttnatanant nnagtnaata antgcancan ctggangcnt nccnnatgat agnagacgaa 1140 gaangtgtnn gcatncnnnn agcatcgtgg aggtggcaan aaaannttgn tttgntnatg 1200 ncggaatgng ggggnccacc cngcaatgtg tatcntnnnc tgaatacatn agnttannta 1260 agngaacccg cngnantgat ccatcnaant anccgcngga aaagaaatca nccganattc 1320 nncnantagt ggcgagcgaa cgcggaggag cctgtatgtk atagttgtcg ggataggaga 1380 aggaattgga aacttccgcc atacgtgggt gagtagcccc gtatccgaaa ttccggcagt 1440 ggtactaagc atacgacaag tagggcggga cacgagaaat cctgtctsaa gatgggggga 1500 ccatcctcca aggctaaata ctcatcatcg atccncnnnc gatagtgaac cagtaccgtg 1560 agggaaaggc gaaaagaacc ccgggagggg agtgaaatag aacctgaaac ctgatgcata 1620 caaacagtgg gagcacttta tggtgtgact gcgtaccttt tgtataatgg gtcaacgact 1680 tacattcagt agcgagctta accggatagg ggaggcgtag ggaaaccgag tcttaatagg 1740 gcgtcatagt tgctgggtgt anacccgaaa ccgagtgatc tatccatggc cangttgaag 1800 gtgccgtaac aggtactgga ggaccgaacc cacgcatgtt gcaaaatgcg gggatgagct 1860 gtggatangg gtgaaaggct aaacaaactc ggagatagct tggttctccc cgaaaactat 1920 ttaggtngtg cctnatgtnt cacttncggg ggtaaaacac tgtnatggct anggggtcat 1980 tgnanttacc nacctatggn aaactnnnaa tacccnaaag tgcnatcatg ggaaacnnac 2040 cnnnngntgc aaacgtncnt tngncngnan ggnaacnncc cnanacnccc tctnnggncc 2100 naatgnnnna nnaaggggna aaanaaanng nagnatgcnc ntagngcatg nnanggnagn 2160 gttnngnagg cntnanaagg tgtttngtan nnaatgnngn aggnatcana agtncnaatg 2220 ttacatnagt ancnataaan cgggtnaaaa gcccgctcnc ngaaagccca aggtttcntg 2280 ngcaacgttc atcggcgcag ggtgagtcgg cccctaaggc gaggcagaga tgcgtagtcg 2340 atgggaaacg ggttaatatt cccgtacttg atttaagtgc gatgtgggga cggagaaggt 2400 taggtcagca aactgttgga atagtttgtt caagccggta ggtggaagga ttaggcaaat 2460 ccggttcttc ataacaccga gaagtgataa cgagtatcta cggatacgaa gtgactgata 2520 ccacgcttcc aggaaaagcc actaagctct agcttaaatc gaaccgtacc gcaaaccgac 2580 acaggtgggc aggatgagaa ttctaaggcg cttgagagaa ctcgggagaa ggaactcggc 2640 aaattgatac tcgtaacttn cgggnagaag gtatgccctc tgaggtgaag gatttactcc 2700 gtaagctttg gagggtcgca gcagaatcgg tggctgcgac tgtttattaa aaacacagca 2760 ctctgcaaac acgaaagtgg acgtataggg tgtgacgcct gcccggtgct ggaaggttaa 2820 ttgaagtatg tgagagcatc ggatcgaagc cccagtaaac ggcggccgta actataacgg 2880 tncctaangt agcgaanttc cttgtcngnt aagttccgan ccgcacnaat ggcgtaacga 2940 tggccacnct gtnnnctacc gngactcngc gaagttnaag tggttgtgat tcatgcaatc 3000 tactnntgnt gatntacctg aaagacccac ctgcacctgn taatgnanct ttngcnngcg 3060 naacttggct atttcgccta tntgnactga tcacgtgngg aangntcaaa aagcaccaga 3120 cgnttccttt nttgngnanc ncgtnccctg gtttggccac gntacctgtc ncnagaggna 3180 ataacccccc gtaccgcnnn nccnnngnnt cccggnanat tgnganctgg gnnngnaaac 3240 cccaaagggc nnncnngnct tgacatntcc anggncnngn gnnntnaaaa ancnttnnnt 3300 tttttttaaa nnnaaagacc tncccccctn ntttcttccn caangnntnn ctttgcaaag 3360 anncaaangn cnaaggctcn cananaaggg tntnnccgcg gggncttttc tnntccnnac 3420 nntgngggnt ggngnngggn naactnnnac cannctncnc naaaaagggn ntntttcttg 3480 ggggaaaccc gnggggtncn nnnaatnccc cnattnttcc cngnggntcc anannaacnc 3540 3540 <210> 26 <211> 2958 <212> DNA <213> Bacteroides vulgatus <400> 26 nccnnccnnn nnnaanntnn tnnnnnnnan nttcnnnnnn ggggnnnncn agtttnnnnc 60 cnnnnnnnan nnnnngggcn tnnnnnnccc ntttttttnn nnaccagccn cgtcaaagcc 120 natgggagcc ngggggtmcc tgaagntgcg taaccscgaa ggagcgnccc tasscgtaaa 180 actggtsmcc ctggggctaa gtcgtnnaam aaggtagccg tnacnnnnnn cggaaaggtg 240 cggctggaac acctcccttt cctggtangr ggacgaaaga tcgacaaggt tttttgttcc 300 tttgtactgc tggtgttgtt tattcaaaga taatgattcc gtaatgatac ggaagagaga 360 aagtaagaag ccgggtctaa tcaaacagac taggttgaac ttagtcctat agctcagttg 420 gttagagcgc tacactgata atgtagaggt cggcagttca actctgcctg ggactacgaa 480 cggaatcaag ttcgggggat tagctcagct ggctagagca tctgccttgc acgcagaggg 540 tcaacggttc gaatccgtta ttctccactc catcggaaac gtaagaaacg aagatgaacg 600 atctatgaca tattgtacaa gcaaacagta attttagtag taaagagctg aaagtatata 660 tcatncccgc tggcacgcaa gtgtgagcgn aatggnataa ggaaagtaaa raagggcgca 720 tggcggatgc cttggctctc ggaggcgatg aaggacgtga taagctgcga taagctttgg 780 gnaggtgcaa atgacctttg atccagagat ttccgaatgg gacaacccaa tatcttgaag 840 aggtattatc caacgttgtt ggaggctaac gcagggaact gaaacatctt agtacctgca 900 gcaaaagnaa ataaacaana naccccgtnt tnnnnnnnan nnangngnan canttntncn 960 ncnncantaa nngggcatgg gkgntaaggg ncngtggccn aanagnagaa gaatcnggng 1020 cacncntngn taaggtcctg naatgacagn taagttnana ntaanaaagt atgantgcta 1080 akacagctag gatgttggnt tgtagrakcm sccmttcnat ctctnagaag agntgsgtaa 1140 cagtctacac tagtmcgagg aagtcgggcg tggtataata atmgggtatt aagttgtcta 1200 cygaagcagt gggatcatta atatkatcgg taggggagca ttccagtcag cgtcgaaggm 1260 gtaccgtsag gtattmtngg agcgtctggw aaagcaaatg twggtataag taacgataaa 1320 gggggcgggn naaaccccct cgccgaaaga mtaaggtttc ctkatcaacn gctaatcgga 1380 tcagggtcag tcggstcsta aggctcagcc gaacggcgat gccgatggca raaacggtta 1440 atattccgtt actaccttca ggagtgacgt ggmgacgcag tgangntgac agctgccgcc 1500 atctracgga agntatgatg gttgaagggt gtaggagtyr atcatggcmg gcawatccac 1560 catgagcatn ccgwacctga cangtatgcc gtcstccttc gggaacaagg ctaatagcts 1620 cgcrataagc mtgctgccga gaaaanctcc gmtmaacttm mtcctgcagg ymcccstacm 1680 gcaaancgga cacacgtagt yggngngatk awtmttctaa ggcgcctntn gagtgattnc 1740 ackgttaarn gaactnaggg caaactngca ccctnktdmc ttnctgggnn rwaaarrgtc 1800 nnccymcycn ttttwtnkkk ttaggscsca gtakaatttc ggtccmgsca accttktwwt 1860 aamnnaaaaa mamaagnggc tgntgccann naatmagaaa raatccrktw tmcakcctda 1920 cmccnttgcc cgggtgctna ganngatang gttaagagra gatgtcatcg antaararaa 1980 gcgttgaatt kaagccccag taamcggcgg csgtaactat aacggtccta aggwagcgna 2040 aattncnttg tcgggtaagt tccgmcctgc mcsaatggtg taatgatctg racgctgtct 2100 caaccgtgag ctcagtgaaa ttgtagtatc ggtgaagatg ccgattaccc scratgggac 2160 gaaaagaccc cgtgamcctt tactatatcg ttaatcattg aatctgggca cgtgatgtgt 2220 aggatakgtc gaagngcntt tgaascaggt acrccagtat ttgtggagcc gctrttgaaa 2280 tacgaccctt tatkktktgc tggattytaa cgccgtngna acggrgacat tgnattggtg 2340 ccnggtastn ttgrgctggg gatggycngc ctmacaaaag cgtaacggrg ggcytcntaa 2400 aggtgtcgcc ncntccggcs gattggctwr ccggccattc cttwgaktkt aatgngcatw 2460 agcggtcgcc ttgtactsgk takwctragc aagtcnngmt ywggwaaagg aaanckrgag 2520 ncantatttt gtgcwtnccn acgccggntg ggganacgtt tnttttccrt atggttanag 2580 gangmcmttt csccntccng gtannnaann cggatcccca naaagrttaw cbccgtttcg 2640 gtcgggawaa nncaaaggnc nntgataaan nnccnctccc aararcycat atcgacggag 2700 tggtttggca cctcgatgtc ggctcgtcac atcctggggc tggagaaggt cccaagggtt 2760 gggctgttcg cccattaaag tggcacgcga gctgggttca gaacgtcgtg agacagttcg 2820 gtctctatct atcgtgggcg catgaaattt gcgtggctct gacactagta cgagaggacc 2880 gtgttggaca gacctncggt ttgccggttg tgccgccagg tgcattgccg ggtatnctaa 2940 gtncngtatc ggataaaa 2958 <210> 27 <211> 3908 <212> DNA <213> Branhamella catarrhalis <400> 27 ccccgcccgt cncaccatnt gggnagttga tctcaccaga aagtggttag cctaacgcaa 60 gagggcgatc accacggtgg ggtcgatgac tggggtgaag tcgtaacaag gtagccgtna 120 ggggaacctg cggctggatc acctccttaa cgaagttatc tgattggcaa gaatccacaa 180 caagttgttc tttggtaaga tgtttaaaaa cggggtctat agctcagttg gttagagcac 240 cgtgttgata acgcgggggt cataagttca agtcttatta gacccaccat tttggggcca 300 tagctcagtt ggtagagcgc ckgccttgca cgcaggaggt caggagttcg tactctcctt 360 ggwtccacca agcaagttta aacatwraag catacakaag caatttaaat aagatttctt 420 atttatgctt ttattttata aactgacgaa gtttataaca ttatttaaca acatagtatg 480 agtctgggtt aattatttaa ttccaacaaa taattaacct ggtgtttgta cccaatacaa 540 acaccaaaaa agtaaagaga actgaatcaa gcgtaaacat aggtgaatcg ttacacatta 600 cccatacaca ccaaagactt cctagaagtc agactacttg gggttgtatg gtcaagtaat 660 gaagtgcaca tggtggatgc cttgncactc agaggcgatg aaanacgtca taacctgcna 720 taagcntnng ngaggtggca atntccccgt gacccnncga tttcntgaat ggggaaaccc 780 aaccnacatn antttggttt attacccaan ntncttgngt aangcatnnc ccnnnntttt 840 nggngctaaa nccntngggn aaangccntn cnttncccnn aaaaggnnnn ngnttnnttt 900 ncntggnccc ccccgncnnn ttttcnnngc agncnaaaga nncccnnnaa nannnnnttt 960 tnannccnng ttttatttna aaangnngnn gattttatcn cttttttnca nnccangagt 1020 ngggntnggn tattttnttn ntttcccata annaanttcn ngtnggnttn nnccccaacc 1080 cccncccnan naaagaaaag tgnttnaagc gtnggnanan nngngncgtt ntttntcacn 1140 tacccanacc acncaantct tttnagnaag cnnacttntn ntggnatngt cgngtaatga 1200 agtgnanatc nnggnngcct ncccngncan annngatgaa anaagnnatn gcatncnatn 1260 agaancggtg nggtggcnnn ancatntnnn nngccnannt ntttttgngg gggnccancc 1320 ancatnantn ggttnttann cagcncantg tgttnggnan nccnncngga gtaanncatc 1380 ncantacccc cccnannaga catcanatna tattcngtca gtagcngcga gcgaacacgg 1440 aggagccgat yaattttwyw gtagcaanat ggcgtgggar gagccaacca tastaggtga 1500 gtagtcctgt atgcgaaact gtttaagcga catattaagt agggcggrac acgagaaatt 1560 actgttctga agtatggggg gaccatcctc ccaaggctaa atactacctg actsaccgna 1620 tagtgaacca gwaccgtgag ggaaaggcga aaagatatct tcgcgctgtt aggsgcagtg 1680 aaatagaacc tgaaaccgtg tgcatacaag acagtcggag cccgaccact taatcgtttt 1740 gaggataaca atgaattttt actacgtcta tgtcatacaa aacgtggata gtcatgatga 1800 gttttatgtt ggttttacca ccaatttaaa acaaagaatt gatgaccata ataatggcac 1860 aacttactca accagagcaa gaacatggcg tattgtttat tgtgaagcct acattaatga 1920 acaagtggca agaaaacgag agcgaaccat aaaaaataat ggtcgtatga gaactttctt 1980 gatgagatcg tgtcaaatca caatttgaca atcagaacga ttaagtggtc gggtswcggc 2040 gtaccttttg tataatgggg tcagcganct tatattctgt mncaanggtt aacncggaat 2100 wggggwgccg tanggataac cgagntttct ntaantaggk cgaatgagtt tgtcagggta 2160 tttacccnaa accgantgat ctatccatga ncnggttnga nanagtgcca tnagancagg 2220 cncctkgagg accnacccct ctgtaccctn ttannantat aagtaanccg taatagctac 2280 actagtncga gtcggcctgc gcggaaaaat gtaacggggc tcaaaaytag gagccgaagc 2340 tgcggattta attgttttca attaaagtgg taggggagcg ttgtgtaagc ctgtgaaggt 2400 gcactgtaag gtgtgctgga ggtatcacaa gagcgaatgc tgacgtgagt aacgacaaaa 2460 cgggtgaaaa gcccgttcgc cggaagacca agggttccag tccaacgtta atcggggctg 2520 ggtgagtcga cccctaaggc gaggccgaaa ggcgtastcg atgggaaatc ggttaatatt 2580 ccgatacttg tttatgatgc gatggaggga cggagaaggt tatgccagcc tggckatggt 2640 tgtccaggtg gaaggatgta gtnctatsac tgaktakgca taatccgctc ggwtattaat 2700 gakatctgwt ascamgccag tttcacctgg caaagtggya aatacsctgc ttycasgaaa 2760 agcttctamk csatagtnct ataacacgaa tcgtacccga atatccgacm caggtggyca 2820 ggtmgagamt acgnctncna aggckctnnn tgagmgtaac tctngcntag rasgnaactc 2880 rsgcwwaatg gtacccgctn acnacyycgk gagaaaskta cgctgaccga tggtkataag 2940 acnttgctst tntngagcnk nangtnnatg gcagtncnnt tgctttagat accaggcttg 3000 cnnnntgcaa ctgtttatta aawacacagc mcttcttntn gctaaacacg aaangctgsm 3060 cstwtmgggt gtgattnggn cctgcccggt gctggaaggt taattgatgg ggttagcgta 3120 agcgaagttt ytgatcgaag ccccagtaaa cggcggccgt aactataacg gtnctaaggt 3180 aannaanttn cttgtngggt aatcttccgt cctncncgan tgncatattg atggcagcgc 3240 tnnctccagn agactactcg gnganaatca aacccncnnn tnataaatgt tnngnnccct 3300 gcntnatnnn angaaacnnn ccccngngna nnctttnacn acnanntntt tnnnaaanna 3360 nnctncncac cctnacactt ctnatacnnn nntcgngnnt cggaannctt ttcnnaatnn 3420 nanttacttc ccactcgtac ggntntntna tncccncacn ctaanncntn gcccacgcnn 3480 ttgtgnngnt nntnntggta ctttctnacn cantnagacn anccanncca angtccnnna 3540 nggancccct cntnnnngnn cnaanttnac nntttnngat nnccnacann naancncccc 3600 ngtnnnaaaa nttttngacn nnaatgagct actcgtaacn cgctanctac ngncctcanc 3660 nntnantcnn natnaagtgt ggtnncacnn nnctnnaaat nntaattncn cntnntntnc 3720 nnntntttct ctctnntacc ttcgnnncnn natananntt tnngctcntc tcnaattacn 3780 gtgcntntta catacnnnat ntacaatntc nngcncgctn ctngtnttac tnngnnttnc 3840 cnctnnncna ctnancattc ntatacnngt tttgnncctt ntccntnact ntacctacgn 3900 nnttcnnt 3908 <210> 28 <211> 3166 <212> DNA <213> Sutterella wadsworthia <220> <221> rRNA <222> (1)..(3166) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 28 ggcgcccacc ggggttcagg ctgggggtca actttaacaa ggtagccctc cggaaggtcc 60 ggctggatca cctcctttaa acagagtcgg ccttgaagcc gccgcaccta cggagtgggc 120 ttcaccagga atcgttggcc ctcccgaaca aggagggggg ttctaggtgg gcttcttaaa 180 ggatggtaag tagaagcaag ggggccagta cttcaagtcc cgccagaatc acctcttttc 240 aagaaagtgg gccttatgca gaatcgacat caagaactgt tgggggcgtt cacacttatc 300 gagttctgtt gaattcaatt cgcccgattc tttaaaaatt tggaaaagta aagcaacaga 360 agctgctgct ttcgaagggt tgagatgctc tttgaaggcg caaaaaggca gcttcagggg 420 ttgtgattgc attcacgaac ccgattcatt gaaagttctt caaagaaccg acatgattca 480 agagaaatac agcgttatag gtcgtcttgt gctttgaagg caaaaggctt cagagttata 540 ggatcaagcg actaagtgta tgtggtggat gccttggcaa aatctacatg gtcgaagaag 600 gtacgtcgtc atcctgtcga aaagccgtgg ggagcctggc aagcgagcca ctgatgccac 660 ggatgtccga atgagggaac tcacccgcaa gggtatcttg aagtgaatac atagcttcaa 720 gaggcgaacg cagcgaactg aaacatctaa gtagctgcag gaaaagaaat caaccgagat 780 tctgaaagta gcggcgagcg aaattggaag agcctgcaag tgatattgaa ttcgatactg 840 gaacggtctg gaaagtccgg ctacagaggg tgacagcccc gtacgggaaa ttgggttcaa 900 ggtactgagc ttgcgagaag tagagcggga cacgtgaatc atccttgctc gaagatgggg 960 ggaccatcct ccaaggctaa atactcgtaa tcgaccgata gcgaacccag tactgtgaag 1020 gaaaggcgaa aagaaccccg ggaggggagt gaaatagatc ctgaaaccgc atacatacaa 1080 acagtaggag cccgcaaggg tgactgcgta ccttttgcat aatgggtcag cgacttacgt 1140 tcaatggcaa gcttaaccga gtaggggagg cgcagcgaaa gcgagtccga acagggcgat 1200 atgagtcgtt gggcgtagac ccgaaaccag atgatctatc catggccagg ctgaaggtgt 1260 ggtaacacac actggagggc cgaaccgact agtgttgcaa aattagcgga tgagctgtgg 1320 ataggggtga aaggctaaac aaatctggag atagctggtt ctccccgaaa actattgagg 1380 tagtgcctcg tgtatggctc cagggggtag agcactgtta tggctagggg gacatggcgt 1440 cttaccaaac catggcaaac tccgaatact tggaagtctg agcacgggag acagagcacc 1500 gggtgctaac gtccgggact caaaagggaa acaacccaga ccgccggcta aggtccctaa 1560 tattgactaa gtggaaaaac gaagtgggaa ggctaaaaca gtcaggaagt tggcttaaac 1620 caccccaatt cactttcaaa gaaaccgtaa tagctaactg atcgagtctt cctgcgccgg 1680 aaaaatgtaa cggggctaag tcaataaccg aagccgcgga ttcgcagcaa tgcagtggta 1740 ggggaagcgt tctgtaagcc tgcaaggcgg atccgcgagg accgctggag gtatcagaag 1800 tgcgaatgct gacatgagta acgttaaagc gggtgaaaag cccctcgcca gcccaagggg 1860 ggacggagtc ctgaaggtca tccgggtgtt ggacatcccg gtttacgagc gtaggaggtt 1920 gacaggcaaa tccgtcaacg gatctccaag gcgaaggatc gaggttgaga gatcaactga 1980 agtgactgaa tgggcttcca ggaaaagcct ctaagcatca gcttggaatt gaccgtaccg 2040 caaaccgaca ctggtgggcg agctgaatat gatcaggcgc ttgagagaac acaggagaag 2100 gaactcggca aattgacacc gtaacttcgg gataaggtgt gcctttgtag tgtgaagaga 2160 gaaacactcc gagcatgaag aggcctcaga gaatcggtgg ctgcaactgt ttactaaaaa 2220 cacagcactc ctgcaaagat gaaagtacga cgtatagggt gtgatgcctg cccggtgctg 2280 gaagattaat tgatggggtg caagctcctg atcgaagtcc cagtaaacgg cggccgtaac 2340 tataacggtc cctaaaggag cgaaattcct tgtcgggcaa gttccgacct gcagaatggc 2400 aaatgatggc cacactgtct cctcctagac tcagtaagtt gaaatgtttg tgatgatgca 2460 atctcccccg gctagacgga aagaccccat aaccttactg cagctttgca ttggactttg 2520 ataccgatct gtgtaggata ggtgggaggc agtgaagcta ggacgccagt cttagcggag 2580 ccaaccttca aataccaccc tgatttgttt gaggttacta acctaggcag acgtaagccg 2640 attgcggggc accgtgcatc ctaggcagtt tgatgggcgg tctcctccta aagggtaacg 2700 gaggagtgcg aaggtacgct aggtacggtc ggaaatcgtg ctgatagtgc aatggcaaaa 2760 gcgtgcttga ctgcgagacc gacaagtcga gcagatacga aagtaggtca tagtgatccg 2820 gtggctctgc atggaagggc catcgctcaa cggataaaag gtactctggg gataacaggc 2880 tgataccgcc caagagttca tatcgacggc ggtgtttggc acctcgatgt cggctcatct 2940 catcctgggg ctgtagccgg tcccaagggt atggctgttc gccatttaaa gaggtacgtg 3000 agctgggttt aaaacgtcgt gagacagttt tgtccctatc tgccgtgggc gttggaagat 3060 tgacgggggc tgctcctagt acgagaggac cggagtggac tcacctctgg tgtaccggtt 3120 gtcacgccag ggcatcgccg ggtaggctat gtgggaaagg aaaaca 3166 <210> 29 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acti004 <400> 29 tgatggaact tgctt 15 <210> 30 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acti23S01 <400> 30 agggcacaca taatg 15 <210> 31 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acti23S02 <400> 31 acgctgttgt tggtg 15 <210> 32 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acti3(Acti I) <400> 32 atacacagta cttcg 15 <210> 33 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acti002 <400> 33 atagtgttgc aaggc 15 <210> 34 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acti003 <400> 34 tgaaaagcca gggga 15 <210> 35 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acti1(Acti 23) <400> 35 gggcacacat aatga 15 <210> 36 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acti2(Acti M) <400> 36 cggggtactc tatac 15 <210> 37 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acti23S03 <400> 37 gtaggtatgt atctt 15 <210> 38 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acti23S04 <400> 38 tactgagatc cgata 15 <210> 39 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acti001 <400> 39 aggtattgca acatg 15 <210> 40 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe ActiIT01 <400> 40 cagaagtagc tgcct 15 <210> 41 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe ActiIT02 <400> 41 agaagtagct gccta 15 <210> 42 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe ActiIT03 <400> 42 gaagtagctg cctaa 15 <210> 43 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe ActiIT04 <400> 43 aagtagctgc ctaac 15 <210> 44 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe ActiIT05 <400> 44 agtagctgcc taact 15 <210> 45 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas001 <400> 45 tgactcgtgc ccatg 15 <210> 46 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas002 <400> 46 taccggggtt aaaag 15 <210> 47 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas003 <400> 47 atcagtgatc tgaga 15 <210> 48 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas004 <400> 48 gagacgaagc accat 15 <210> 49 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas011 <400> 49 agttgataca ggtag 15 <210> 50 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas013 <400> 50 ggccccatcc ggggt 15 <210> 51 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas23S03 <400> 51 cagttggaag cagag 15 <210> 52 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas005 <400> 52 gttcttgatt cattg 15 <210> 53 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas008 <400> 53 cagcccaaaa gttga 15 <210> 54 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas009 <400> 54 aaactgcagg gcaca 15 <210> 55 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas010 <400> 55 atactacctg acgac 15 <210> 56 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas1(Anas7) <400> 56 aaagtgcagg gcaca 15 <210> 57 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas2(AnasM) <400> 57 tggattgtgg tgaaa 15 <210> 58 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas3(Anas7) <400> 58 tagcgttctg cgagg 15 <210> 59 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas4(AnasM) <400> 59 ttaaaagact ggtat 15 <210> 60 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas006 <400> 60 atccaatcat gatca 15 <210> 61 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas007 <400> 61 aagcatgaaa gcgca 15 <210> 62 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas014 <400> 62 gaggcgggag ccgag 15 <210> 63 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas23001 <400> 63 ccccatccgg ggttg 15 <210> 64 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas23S01 <400> 64 tggcgtcagg aggcg 15 <210> 65 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas23S02 <400> 65 ataaggggcg cttga 15 <210> 66 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas23S04 <400> 66 tcacacgcaa gtgtg 15 <210> 67 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas23S05 <400> 67 gctgagacga agcac 15 <210> 68 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas23S06 <400> 68 ataccggggt taaaa 15 <210> 69 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas23S03 <400> 69 cagttggaag cagag 15 <210> 70 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas012 <400> 70 tagcgttctg cgagg 15 <210> 71 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe AnasIT001 <400> 71 acagcgcagc atgtg 15 <210> 72 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe AnasIT002 <400> 72 aattagcaac tattt 15 <210> 73 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe AnasIT01 <400> 73 cttccctcag tgatt 15 <210> 74 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe AnasIT02 <400> 74 ttccctcagt gattc 15 <210> 75 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe AnasIT03 <400> 75 tccctcagtg attca 15 <210> 76 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe AnasIT04 <400> 76 ccctcagtga ttcaa 15 <210> 77 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe AnasIT05 <400> 77 cctcagtgat tcaag 15 <210> 78 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bf 011 <400> 78 gtcgaacctg acagt 15 <210> 79 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bacf1(Bf23) <400> 79 ggtaaccgaa gcgta 15 <210> 80 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bacf2(Bf) <400> 80 ctcggaaaac ggtaa 15 <210> 81 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bf 001 <400> 81 agcgatgttg aaaac 15 <210> 82 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bf 002 <400> 82 tcaaccatct atagc 15 <210> 83 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bf 003 <400> 83 aacaagagaa aaaca 15 <210> 84 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bf 004 <400> 84 cgataccgcg accta 15 <210> 85 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bf 005 <400> 85 tatatcgaac cattt 15 <210> 86 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bf 006 <400> 86 gaatctggcg ataaa 15 <210> 87 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bf 007 <400> 87 tgcaaatgac ctttg 15 <210> 88 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bf 008 <400> 88 caacttggtt ggagg 15 <210> 89 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bf 009 <400> 89 acccatgtta cggca 15 <210> 90 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bf 010 <400> 90 agttgaccta acgaa 15 <210> 91 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bf 012 <400> 91 tgaacggatc tgtgt 15 <210> 92 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bacf3(Bf I) <400> 92 ggttcagatc ctttt 15 <210> 93 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Car 006 <400> 93 aaccctggtg aaggg 15 <210> 94 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Car 007 <400> 94 atatgaagat atgtg 15 <210> 95 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Car 008 <400> 95 tagattgact tacgg 15 <210> 96 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Car 009 <400> 96 gtaaagtttt actac 15 <210> 97 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Car2(CarM) <400> 97 ccagcacact gttgg 15 <210> 98 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Car3(CarI) <400> 98 aaagagagaa cagca 15 <210> 99 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Car 001 <400> 99 ttggcgacaa caggc 15 <210> 100 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Car 002 <400> 100 gccccgggaa gctga 15 <210> 101 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Car 003 <400> 101 tagactgcgg aagcg 15 <210> 102 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Car 004 <400> 102 aattaagttg cgtat 15 <210> 103 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Car1(Car23) <400> 103 ccatacacaa tgaat 15 <210> 104 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Car 005 <400> 104 tactcgttgt cgacc 15 <210> 105 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Chr23S04 <400> 105 ggcatattta gatga 15 <210> 106 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Chr001 <400> 106 cttaggtgat cactt 15 <210> 107 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Chr003 <400> 107 taacccctta gatta 15 <210> 108 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Chr004 <400> 108 tcaaacctca aacta 15 <210> 109 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Chr005 <400> 109 aagaaatcga agaga 15 <210> 110 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Chry1(Chry23) <400> 110 atttagatga taaat 15 <210> 111 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Chry2(Chry7) <400> 111 taatcttact agcga 15 <210> 112 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Chr23S05 <400> 112 atcgtgaggt tacga 15 <210> 113 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Chry3(ChryI) <400> 113 tccttgagtg cagag 15 <210> 114 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Chr002 <400> 114 agcacagctt tggtt 15 <210> 115 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.ramosa04 <400> 115 ccagtgtgtg aggag 15 <210> 116 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.ramo 004 <400> 116 cccgggaagg ggagt 15 <210> 117 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.ramosa01 <400> 117 ggtgaagtat tagta 15 <210> 118 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.ramosa02 <400> 118 atgtacaggc atagg 15 <210> 119 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.ramosa03 <400> 119 tgagagacat gcacg 15 <210> 120 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.ramosa05 <400> 120 gtattggagt tgcta 15 <210> 121 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.ramo 001 <400> 121 tagttgatga tagta 15 <210> 122 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.ramo 002 <400> 122 gcttatctgt ggatg 15 <210> 123 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.ramo 003 <400> 123 ggaatccctc cttgt 15 <210> 124 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Com 004 <400> 124 tagggcgtcc agtcg 15 <210> 125 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Com 005 <400> 125 cgcagagtac agctt 15 <210> 126 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Com 006 <400> 126 gtaccgatgt gtagt 15 <210> 127 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Com 007 <400> 127 gaacttgaac aaagg 15 <210> 128 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Coma2(Com 7) <400> 128 tgagctagag aaaag 15 <210> 129 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Coma3(Com M) <400> 129 atccgccggg cttag 15 <210> 130 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Coma1(Com23) <400> 130 aaaaccgact ggtgg 15 <210> 131 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Com 001 <400> 131 gctgacggaa agaga 15 <210> 132 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Com 002 <400> 132 ctcttgacag aaatg 15 <210> 133 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Com 003 <400> 133 aagaattcat tcaca 15 <210> 134 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Coma4(Com I) <400> 134 acgcgcgagg tgaga 15 <210> 135 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.diph003 <400> 135 accatcttcc caagg 15 <210> 136 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.dipht 01 <400> 136 taactagata agaaa 15 <210> 137 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.diph001 <400> 137 accacgcagc agttt 15 <210> 138 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.diph002 <400> 138 cgagtcggta gggta 15 <210> 139 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.diph004 <400> 139 tgtttgttct ttgat 15 <210> 140 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.diph005 <400> 140 aaaatcagaa aaaca 15 <210> 141 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.diph006 <400> 141 ggaaaatcag aaaaa 15 <210> 142 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ko001 <400> 142 gaacgttact aacgc 15 <210> 143 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Koxy1(Ko M) <400> 143 ccggaacgtt actaa 15 <210> 144 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Koxy3(Ko M) <400> 144 aagagcgcca gctca 15 <210> 145 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ko001 <400> 145 tttgaagttc taact 15 <210> 146 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ko002 <400> 146 aagagcgcca gctac 15 <210> 147 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ko002 <400> 147 tatctaccgc gggcg 15 <210> 148 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ko003 <400> 148 gatgaagacc tcaaa 15 <210> 149 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ko003 <400> 149 ttacgggttg tcatg 15 <210> 150 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Koxy2(Ko I) <400> 150 cgcgacacga cgatg 15 <210> 151 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ochr04 <400> 151 ggaccaggcc agtgg 15 <210> 152 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ochr05 <400> 152 gaccaggcca gtggc 15 <210> 153 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ochr004 <400> 153 gttgattgac acttg 15 <210> 154 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ochr005 <400> 154 taccgctcac gagcc 15 <210> 155 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ochr007 <400> 155 gggtccggag gttca 15 <210> 156 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ochr1(Ochr 23-1) <400> 156 cggcgcgtga gcgag 15 <210> 157 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ochr2(Ochr 7-1) <400> 157 gaacacctgt tgtcc 15 <210> 158 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ochr4(Ochr 23-2) <400> 158 tcgtcggccc atgtg 15 <210> 159 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ochr5(Ochr 7-2) <400> 159 ttagtgtatc gagca 15 <210> 160 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ochr002 <400> 160 cttcgggctg atgat 15 <210> 161 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ochr003 <400> 161 aggccagtca gcctg 15 <210> 162 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ochr006 <400> 162 gttggttctg ataca 15 <210> 163 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ochr008 <400> 163 cagttggaag cagag 15 <210> 164 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ochr3(Ochr I) <400> 164 gatccgacga tttcc 15 <210> 165 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ochr001 <400> 165 taggaaagac gcagt 15 <210> 166 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pep002 <400> 166 actagggaga gctca 15 <210> 167 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pep003 <400> 167 gcttagtaaa gcaag 15 <210> 168 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pep004 <400> 168 tactaacatg tgacc 15 <210> 169 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pep005 <400> 169 aagcagagag agctc 15 <210> 170 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pep006 <400> 170 cgaacggtga ggccg 15 <210> 171 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pep007 <400> 171 gtagatgttg attat 15 <210> 172 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pep23S02 <400> 172 gtcgaatcat ctggg 15 <210> 173 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pep23S03 <400> 173 taaaacgtat cggat 15 <210> 174 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pep1(Pep23) <400> 174 actaaataaa ccagg 15 <210> 175 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pep2(PepM) <400> 175 ataatcaaca tctac 15 <210> 176 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pep3(PepI) <400> 176 tctgtataat agttc 15 <210> 177 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pep001 <400> 177 agaagctgat acgtc 15 <210> 178 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Por 003 <400> 178 agttggtgag cgagc 15 <210> 179 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Por23S08 <400> 179 ctgagctgtc gtgca 15 <210> 180 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Por 001 <400> 180 gtttttgtga gtgga 15 <210> 181 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Por 002 <400> 181 tgatgggtgg ggttg 15 <210> 182 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Por1(Por7) <400> 182 gttggatgtt atcat 15 <210> 183 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Por2(PorM) <400> 183 cgggcagcta aaacc 15 <210> 184 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Por 004 <400> 184 acctatgagt actat 15 <210> 185 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Por3(PorI) <400> 185 tgtttgtgcg acgtg 15 <210> 186 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.anae003 <400> 186 aggaggaaga gaaag 15 <210> 187 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.anae004 <400> 187 gcgaaaggaa aagag 15 <210> 188 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.anae001 <400> 188 tgcattacta agtga 15 <210> 189 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.anae002 <400> 189 gtaaggtcga taccc 15 <210> 190 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.magn002 <400> 190 catgcaacga tccgt 15 <210> 191 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.magn001 <400> 191 tagttgaaaa tagta 15 <210> 192 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.magn003 <400> 192 cagcacgtga atatg 15 <210> 193 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe f.necro01 <400> 193 tttcgcagac gtaag 15 <210> 194 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe f.necro02 <400> 194 gttttcttgc gctgt 15 <210> 195 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe f.necro03 <400> 195 ccgtattcat gtcaa 15 <210> 196 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe f.necro05 <400> 196 ctgcaagcta tttcg 15 <210> 197 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe f.necro06 <400> 197 cagacgtaag caaag 15 <210> 198 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe f.necro07 <400> 198 cctgtattgg tagtt 15 <210> 199 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.vulga004 <400> 199 agaggaggct tagtg 15 <210> 200 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.vulga01 <400> 200 atacgtgtta tgtgc 15 <210> 201 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.vulga03 <400> 201 atatccaatg gatat 15 <210> 202 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.vulga006 <400> 202 ggaaacccaa tatcc 15 <210> 203 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.vulga007 <400> 203 gggaaaccca atatc 15 <210> 204 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.vulga008 <400> 204 cactgtttcg actag 15 <210> 205 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.vulga02 <400> 205 ctcacacaga cttgt 15 <210> 206 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.vulga005 <400> 206 gtgggttgca aaata 15 <210> 207 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.aero01 <400> 207 ttccgacggt acagg 15 <210> 208 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.aero03 <400> 208 gtatcagtaa gtgcg 15 <210> 209 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.aero04 <400> 209 ttatccaggc aaatc 15 <210> 210 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.aero02 <400> 210 gagcggggta gttga 15 <210> 211 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.aero005 <400> 211 aatcaaggct gaggt 15 <210> 212 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S.mutan001 <400> 212 taggtattct ctcct 15 <210> 213 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S.mutans01 <400> 213 gaaaaacgaa gggta 15 <210> 214 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S.mutans02 <400> 214 atgactacgt ggtcg 15 <210> 215 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S.mutans03 <400> 215 gtaatgcaag atatc 15 <210> 216 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S.mutans004 <400> 216 ttgtatgcgc ggtag 15 <210> 217 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S.mutans005 <400> 217 cgaaaagtat cgggg 15 <210> 218 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe K.king02 <400> 218 ggttagcaaa ctgtt 15 <210> 219 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe K.king03 <400> 219 ccagtaggtg gaaag 15 <210> 220 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe K.king04 <400> 220 aacaccgaga cgtga 15 <210> 221 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe K.king09 <400> 221 tattcaatgc gatgg 15 <210> 222 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe K.king01 <400> 222 tgattcaatg cgatg 15 <210> 223 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe K.king05 <400> 223 tataattaaa cgcat 15 <210> 224 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe K.king06 <400> 224 aatgttgtcg atttg 15 <210> 225 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe K.king07 <400> 225 aggcaacaaa tcgaa 15 <210> 226 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe K.king08 <400> 226 tatcaactaa tcttg 15 <210> 227 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.ovatus01 <400> 227 tagaaggaag cattc 15 <210> 228 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.ovatus02 <400> 228 ccaatgttgt tacgg 15 <210> 229 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.ovatus005 <400> 229 tgtaggacca cgatg 15 <210> 230 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.ovatus003 <400> 230 ggaccgaacc gataa 15 <210> 231 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.ovatus004 <400> 231 ggacacgagg aatct 15 <210> 232 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.ovatus006 <400> 232 tgaaggaatg tcatc 15 <210> 233 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.ovatus007 <400> 233 cccacgatag ataga 15 <210> 234 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.thetaio006 <400> 234 gctaacgcag ggaac 15 <210> 235 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.thetaio01 <400> 235 ttattgtact actgg 15 <210> 236 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.thetaio02 <400> 236 atcaggtaga caagg 15 <210> 237 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.thetaio03 <400> 237 ttgtcgttgc caata 15 <210> 238 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.thetaio04 <400> 238 cagtgttgga atgtt 15 <210> 239 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.thetaio05 <400> 239 actatactat agtca 15 <210> 240 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.diffc005 <400> 240 gttcgtccgc ccctg 15 <210> 241 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.diffc001 <400> 241 gcatatatat ttagt 15 <210> 242 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.diffc002 <400> 242 gatatgacat ctaat 15 <210> 243 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.diffc003 <400> 243 tttcggggag ttgca 15 <210> 244 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.diffc004 <400> 244 catgtggaca gtatg 15 <210> 245 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe H.aphro003 <400> 245 ggtgaagaac ccact 15 <210> 246 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe H.aphro001 <400> 246 tgggagtggg ttgtc 15 <210> 247 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe H.aphro002 <400> 247 taacaaaccg gaaac 15 <210> 248 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe H.aphro004 <400> 248 atcattatct gaatc 15 <210> 249 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe H.aphro005 <400> 249 agaaatcaac cgtag 15 <210> 250 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe H.aphro006 <400> 250 attagcggat gactc 15 <210> 251 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe H.aphro007 <400> 251 aacccagtgg gtgaa 15 <210> 252 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe H.aphro008 <400> 252 aaacccagtg ggtga 15 <210> 253 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe H.aphro009 <400> 253 gaaacccagt gggtg 15 <210> 254 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe N.gono005 <400> 254 tatcaaagta gggat 15 <210> 255 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe N.gono006 <400> 255 agtcaacggg taggt 15 <210> 256 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe N.gono002 <400> 256 aacctctcgc aagag 15 <210> 257 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe N.gono003 <400> 257 catagtattt gggtg 15 <210> 258 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe N.gono004 <400> 258 ttgtatcaga cttaa 15 <210> 259 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe N.gono001 <400> 259 aatgagtttg ttttg 15 <210> 260 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe N.gono007 <400> 260 caatgagttt gtttt 15 <210> 261 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe N.gono008 <400> 261 cgtaactata acggt 15 <210> 262 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.corro005 <400> 262 ggataggaga aggaa 15 <210> 263 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.corro006 <400> 263 actcatcatc gatcc 15 <210> 264 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.corro001 <400> 264 agtcgtagag cggag 15 <210> 265 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.corro002 <400> 265 agatccgccc aggta 15 <210> 266 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.corro003 <400> 266 gttgctgcat cttgc 15 <210> 267 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.corro004 <400> 267 gcaggattcg gacac 15 <210> 268 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.vulga03 <400> 268 agtcagcgtc gaagg 15 <210> 269 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.vulga07 <400> 269 cgaatgcgca tcagt 15 <210> 270 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.vulga01 <400> 270 catcttgaga tgtgc 15 <210> 271 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.vulga02 <400> 271 agtcgggcgt ggata 15 <210> 272 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.vulga04 <400> 272 acgctaatcg gatca 15 <210> 273 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.vulga05 <400> 273 gaccgataga gcatg 15 <210> 274 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.vulga06 <400> 274 tgacacactg taact 15 <210> 275 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.vulga08 <400> 275 attgtcatga gccac 15 <210> 276 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.vulga09 <400> 276 aatttgcgtg gctct 15 <210> 277 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.vulga10 <400> 277 ctccatcgga aacgt 15 <210> 278 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.vulga11 <400> 278 actccatcgg aaacg 15 <210> 279 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.vulga12 <400> 279 gggactacga acgga 15 <210> 280 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.catar005 <400> 280 atatcttcgc gctgt 15 <210> 281 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.catar001 <400> 281 agtctgggtt aatta 15 <210> 282 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.catar002 <400> 282 acaagttgtt ctttg 15 <210> 283 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.catar003 <400> 283 aacataggtg aatcg 15 <210> 284 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.catar004 <400> 284 aagtaatgaa gtgca 15 <210> 285 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.catar006 <400> 285 gaggataaca atgaa 15 <210> 286 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.catar007 <400> 286 cgaatgagtt tgtca 15 <210> 287 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.catar008 <400> 287 acccgaatat ccgac 15 <210> 288 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.catar009 <400> 288 gacccaccat tttgg 15 <210> 289 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.catar010 <400> 289 ataatggggt cagcg 15 <210> 290 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.catar011 <400> 290 agcctgtgaa ggtgc 15 <210> 291 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.catar012 <400> 291 aagaattgat gacca 15 <210> 292 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S.wad 02 <400> 292 gctccgacaa gaact 15 <210> 293 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S.wad03 <400> 293 cgagttgttg aattc 15 <210> 294 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S.wad04 <400> 294 gtcgtcttgt gcttt 15 <210> 295 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S.wad 01 <400> 295 ctcagtaaga cgttt 15 <210> 296 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acii1(Acii23) <400> 296 aacctggctg gtggc 15 <210> 297 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acii2(AciiM) <400> 297 gacacttttg tgtca 15 <210> 298 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acii3(Aci I) <400> 298 gttgggtggt tgcct 15 <210> 299 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe SeM01 <400> 299 gatagataac aggtg 15 <210> 300 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe SeM02 <400> 300 agggttcacg cccag 15 <210> 301 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Se1(Se 23) <400> 301 ttctctcttg agtgg 15 <210> 302 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Se2(Se M) <400> 302 cgtgctgttg gagtg 15 <210> 303 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Se3(Se I) <400> 303 gctatttatt ttgaa 15 <210> 304 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bur(Bur23) <400> 304 ttgttagccg aacgc 15 <210> 305 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bur01 <400> 305 gggtgtggcg cgagc 15 <210> 306 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bur001 <400> 306 gccaggaggg tgaag 15 <210> 307 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Styp(Styp23) <400> 307 gcctgaatca gcatg 15 <210> 308 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Styp01 <400> 308 gctgaggata cggtt 15 <210> 309 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Styp02 <400> 309 ccgcaaaaca agcag 15 <210> 310 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Styp03 <400> 310 acgattgacg gagcg 15 <210> 311 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Sal.typ001 <400> 311 tcgcgccgtc acagt 15 <210> 312 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E coli 003 <400> 312 ctgaagcgac aaatg 15 <210> 313 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Eco1(Eco23) <400> 313 gagcctgaat cagtg 15 <210> 314 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Eco2(Ecoli) <400> 314 gttagcggta acgcg 15 <210> 315 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E coli 001 <400> 315 gttagcggta acgcg 15 <210> 316 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E coli 002 <400> 316 atgcacatat tgtga 15 <210> 317 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Kpneu(Kpneu 23) <400> 317 gtacaccaaa atgca 15 <210> 318 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe K.pneu002 <400> 318 gctgagacca gtcga 15 <210> 319 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe K.pneu001 <400> 319 acgctggtgt gtagg 15 <210> 320 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Kpneu01 <400> 320 accttcgggt gtgac 15 <210> 321 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pm <400> 321 gttaccaaca atcgt 15 <210> 322 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pm002 <400> 322 ggcgacggtc gtccc 15 <210> 323 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pm003 <400> 323 gatgacgaac cacca 15 <210> 324 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pm004 <400> 324 tgaagcaatt gatgc 15 <210> 325 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pm001 <400> 325 aaggctaggt tgtcc 15 <210> 326 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pm005 <400> 326 taaagtccct cgcgg 15 <210> 327 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pm01 <400> 327 aggcagagtg attag 15 <210> 328 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Strepp(StreppM) <400> 328 taggactgca atgtg 15 <210> 329 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Strepp01 <400> 329 atgtggtaca gacac 15 <210> 330 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Strepp02 <400> 330 ggttaaacgc tagaa 15 <210> 331 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Strepp03 <400> 331 caggatactg ctaag 15 <210> 332 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Strepp04 <400> 332 gagtaaactc ttcgg 15 <210> 333 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Vvul02 <400> 333 gttgacgatg catgt 15 <210> 334 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe VvulM <400> 334 agtaacagcc acttg 15 <210> 335 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe V.vul001 <400> 335 atagctcaat gaagc 15 <210> 336 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe V.vul002 <400> 336 ggcgccatag tctct 15 <210> 337 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Vvul 01 <400> 337 tttacatgtg ttaga 15 <210> 338 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Vvul 03 <400> 338 gttctatgaa cattg 15 <210> 339 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.aeru001 <400> 339 gaagtgccga gcatg 15 <210> 340 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pa03 <400> 340 ggatctttga agtga 15 <210> 341 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pa <400> 341 gtttcccgtg aaggc 15 <210> 342 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.aeru002 <400> 342 gtgtcacgta agtga 15 <210> 343 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.aeru003 <400> 343 agtcgtcttt tagat 15 <210> 344 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.aeru004 <400> 344 actccgtaag ctctg 15 <210> 345 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pa01 <400> 345 taggataacc taggt 15 <210> 346 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pa02 <400> 346 taagcttcat tgatt 15 <210> 347 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ah <400> 347 ggcgcctcgg taggg 15 <210> 348 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ae.hy 001 <400> 348 taagccgtga gcagt 15 <210> 349 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ae.hy 002 <400> 349 catcttggaa gttag 15 <210> 350 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ae.hy 003 <400> 350 tcaaaccagg caccg 15 <210> 351 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ae.hy 004 <400> 351 gattcacgct aagcg 15 <210> 352 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ae.hy 005 <400> 352 acggtgcgga agcca 15 <210> 353 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ah01 <400> 353 cacgaaaaca acctt 15 <210> 354 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe LM <400> 354 gggtgcaagc ccgag 15 <210> 355 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Lm01 <400> 355 agtatcctta cgtga 15 <210> 356 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Lm02 <400> 356 gtgaggaagg cagac 15 <210> 357 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Lm03 <400> 357 ggctttccct ccaga 15 <210> 358 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Lm04 <400> 358 ccgcttctca cgaag 15 <210> 359 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.faecium002 <400> 359 ttacgattgt gtgaa 15 <210> 360 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.faecium003 <400> 360 atagcacatt cgagg 15 <210> 361 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Enfcium1(Enfaeci23) <400> 361 gttctttcag atagt 15 <210> 362 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Enfcium2(Enfaeci M) <400> 362 ctgaagagga gtcaa 15 <210> 363 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.faecium001 <400> 363 gctgatcata cgatc 15 <210> 364 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.faecium004 <400> 364 cttcttttct taagg 15 <210> 365 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S.aureus004 <400> 365 gattgcacgt ctaag 15 <210> 366 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S.aureus005 <400> 366 aatccggtac tcgtt 15 <210> 367 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Saure03 <400> 367 tcttcgagtc gttga 15 <210> 368 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Saur <400> 368 gttaacgccc agaag 15 <210> 369 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S.aureus001 <400> 369 aggacgacat tagac 15 <210> 370 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S.aureus002 <400> 370 aaaatgttgt ctctc 15 <210> 371 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S.aureus003 <400> 371 cgaagcgtgc gattg 15 <210> 372 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Saure01 <400> 372 aagcagtaaa tgtgg 15 <210> 373 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Saure02 <400> 373 gagaagacat tgtgt 15 <210> 374 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Saureus01 <400> 374 atatcagaag gcaca 15 <210> 375 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Saureus02 <400> 375 acaaaggacg acatt 15 <210> 376 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Saureus03 <400> 376 tcttcgagtc gttga 15 <210> 377 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Nm002 <400> 377 agatgtgaga gcatc 15 <210> 378 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Nm1(Nm) <400> 378 ccctggaggg tcgca 15 <210> 379 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Nm2(Nm-1) <400> 379 tttgaattga accgt 15 <210> 380 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Nm001 <400> 380 gtttactggc atggt 15 <210> 381 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Nm01 <400> 381 taaagcaatg atccc 15 <210> 382 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Nm02 <400> 382 ccgggtcttc ttaac 15 <210> 383 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu011 <400> 383 tggagagcat tttat 15 <210> 384 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu012 <400> 384 gtgattttga ggtga 15 <210> 385 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu013 <400> 385 agatggtaaa gaaga 15 <210> 386 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu001 <400> 386 cctcaagatg agttt 15 <210> 387 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu002 <400> 387 gaagcccgtt gaaga 15 <210> 388 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu003 <400> 388 gcagtaatgc gtgaa 15 <210> 389 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu004 <400> 389 ttgtcttgac catat 15 <210> 390 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu005 <400> 390 accatataat ctgag 15 <210> 391 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu006 <400> 391 tgcccacaca gtttg 15 <210> 392 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu007 <400> 392 caaagtgccc acaca 15 <210> 393 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu008 <400> 393 tgattttgag gtgat 15 <210> 394 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu009 <400> 394 ccaccattta atgat 15 <210> 395 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu010 <400> 395 agcattttat tctgg 15 <210> 396 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.alic001 <400> 396 tggtagccat ttatg 15 <210> 397 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.alic003 <400> 397 ctggaccagc cgagc 15 <210> 398 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.alic006 <400> 398 tcaagaacga aagtt 15 <210> 399 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.alic007 <400> 399 aaggattgac agatt 15 <210> 400 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.alic008 <400> 400 cattaatcaa gaacg 15 <210> 401 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.alic002 <400> 401 ttttcgatgc gtact 15 <210> 402 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.alic004 <400> 402 cagatgtcga aaggt 15 <210> 403 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.alic005 <400> 403 taggacgtta tggtt 15 <210> 404 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.glab001 <400> 404 ctggaatgca cccgg 15 <210> 405 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.glab003 <400> 405 tggcttggcg gcgaa 15 <210> 406 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Primer 1585Fw <400> 406 ttgtacacac cgcccgtc 18 <210> 407 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Primer 23BR <400> 407 ttcgcctttc cctcacggta ct 22 <210> 408 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Primer 23BFw <400> 408 agtaccgtga gggaaaggcg aa 22 <210> 409 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Primer 37R <400> 409 tgcttctaag ccaacatcct 20 <210> 410 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Primer MS37F <400> 410 aggatgttgg cttagaagca 20 <210> 411 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Primer MS38R <400> 411 cccgacaagg aatttcgcta cctt 24 <210> 412 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Primer fun463F <400> 412 gtaattggaa tgagtacaat 20 <210> 413 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Primer fun986R <400> 413 ctacgacggt atctgatcat 20 <210> 414 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Primer 520R <400> 414 gccaaggcat ccacc 15 <210> 415 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> Primer 23S 750F <400> 415 agtagcggcg agcgaa 16 <210> 416 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> Primer 23S 750F(T) <400> 416 agtagtggcg agcgaa 16 <210> 417 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> Primer 970F <400> 417 aactggagga ccgaacc 17 <210> 418 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> Primer 930R <400> 418 awtttgcyga gttcctt 17 <210> 419 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Primer 2960R(T) <400> 419 gcttagatgc tttcagca 18 <210> 420 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Primer 2960RC <400> 420 gcttagatgc tttcagcg 18 <210> 421 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Control Probe BaP1-01 <400> 421 cacggtggat gccct 15 <210> 422 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Control Probe Bap1-03 <400> 422 agtagcggcg agcga 15 <210> 423 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Control Probe BaP1-06 <400> 423 gaccgatagt gaacc 15 <210> 424 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Control Probe BaP2-01 <400> 424 agaacctgaa accgt 15 <210> 425 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Control Probe BaP2-03 <400> 425 actggaggac cgaac 15 <210> 426 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Control Probe BaP2-04 <400> 426 agggaaacaa cccag 15 <210> 427 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Control Probe BaP3 <400> 427 gtaaacggcg gccgt 15 <210> 428 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Position marker <400> 428 aaaaaaaaaa aaaaa 15<110> MEDIGENES <120> DNA chips for Detection of Peptostreptococcus anarobius bacilli <130> P05-0674 <160> 428 <170> KopatentIn 1.71 <210> 1 <211> 3510 <212> DNA <213> Acinetobacter baumannii <220> <221> rRNA (222) (1) .. (3510) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 1 ccatgggagt ttgttgcacc agaagtagct gcctaactgc aaagagggcg gttaccacgg 60 tgtggccgat gactggggtg aagtcgtaac aaggtagccg taggggaacc tgcggctgga 120 tcacctcctt aacgaaagat tgacgattgg taagaatcca caacaagttg ttcttcatag 180 atgtatctga gggtctgtag ctcagttggt tagagcacac gcttgataag cgtggggtca 240 caagttcaag tcttgtcaga cccaccatga ctttgactgg ttgaagttat agataaaaga 300 tacatgactg atgatgtaag ctggggactt agcttagttg gtagagcgcc tgctttgcac 360 gcaggaggtc aggagttcga ctctcctagt ctccaccaga acttaagaga agttcggatt 420 acagaaatta gtaaatagag atttagatct tggtttatta acttctgtga tttaagtatc 480 acggtattaa gcatgacctg acgaaggcat gtttattcat taacagattg gcaaaattga 540 gtctgaaata aattgttcac tcaagagttt agattaagca attaatctag atgaattgag 600 aactagcaaa ttaactgaat caagcgtttt ggtatatgaa tttagattga agctgtacag 660 tgtttaaata cacagtactt cgaactgtat ggatgaatga gagatcattt gttctgttgc 720 tacaccaact tgtaggtgta acgactgttt ggggttgtat agtcaagtaa ttaagtgcat 780 gtggtggatg ccttggcagt cagaggcgat gaaagacgtg atagcctgcg aaaagctccg 840 gggaggcggc aaatatcctt tgatccggag atttctgaat gggggaaccc acctacttta 900 aggtaggtat tgcaacatga atacatagtg ttgcaaggcg aacgagggga agtgaaacat 960 ctcagtaccc ttaggaaaag aaatcaattg agattccctc agtagcggcg agcgaacggg 1020 gatcagccca ttaagttatg tgtgttttag tggaacgctc tgggaagtgc gaacgtagag 1080 ggtgatattc ccgtacacga aagggcacac ataatgatga cgagtagggc gaggcacgtg 1140 aaaccttgtc tgaatatggg gggaccatcc tccaaggcta aatactcctg actgaccgat 1200 agtgaaccag taccgtgagg gaaaggcgaa aagaacccct gtgaggggag tgaaatagat 1260 cctgaaaccg catgcataca agcagtggga gcaccttcgt ggtgtgactg cgtacctttt 1320 gtataatggg tcagcgactt atattcagta gcaaggttaa ccgtataggg gagccgtagg 1380 gaaaccgagt cttaataggg cgtttagttg ctgggtatag acccgaaacc aggcgatcta 1440 tccatgagca ggttgaaggt tgggtaacac taactggagg accgaaccca ctgtcgttga 1500 aaagccaggg gatgacttgt ggataggggg tgaaaggcta atcaagcctg gtgatagctg 1560 gttctccccg aaagctattt aggtagcgcc tcggacgaat accatagggg gtagagcact 1620 gtttcggcta gggggtcatc ccgacttacc aaaccgatgc aaactccgaa tacctatgag 1680 tactatccgg gagacagact gcgggtgcta acgtccgtag tcaagaggaa aacaatccag 1740 accgccagct aaggccccaa aatcatagtt aagtgggaaa cgatgtggga aggcatagac 1800 agctaggagg ttggcttaga agcagccacc ctttaaagaa agcgtaatag ctcactagtc 1860 gagtcggcct gcgcggaaga tgtaacgggg ctaaaactat gtgccgaagc tgcggatttg 1920 acattagtca agtggtaggg gagcgttctg taagccgatg aaggtgtatt gagaagtatg 1980 ctggaggtat cagaagtgcg aatgctgacg tgagtaacga caaaacgggt gaaaaacccg 2040 ttcgccgaaa gaccaagggt tccagtccaa cgttaatcgg ggctgggtga gtcgacccct 2100 aaggcgaggc cgaaaggcgt agtcgatggg aaattggtta atattccaat acttctgtgt 2160 aatgcgatga gaggacggag aaggttaagt cagcctggcg ttggttgtcc aggtggaagg 2220 atgtaggtat gtatcttagg caaatccggg gtactctata ctgagatccg atagcaagct 2280 gtacttgtac agtgaagtgg ctgataccat gcttccagga aaagtctcta agcttcagtt 2340 acacaggaat cgtacccgaa accgacacag gtggtcaggt cgagtagacc aaggcgcttg 2400 agagaactct gctgaaggaa ctaggcaaaa tggtaccgta acttcgggag aaggtacgct 2460 gttgttggtg atggaacttg cttcctgagc tgacgacagc cgcagaaacc aggccgctgc 2520 aactgtttat taaaaacata gcactctgca aacacgaaag tggacgtata gggtgtgatg 2580 cctgcccggt gctggaaggt taattgatgg ggttagcgta agcgaagctc ttgatcgaag 2640 ccccagtaaa cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt 2700 aagttccgac ctgcacgaat ggcataatga tggcggcgct gtctccagca gaggctcagt 2760 gaaatcgaaa tcgctgtgaa gatgcagtgt acccgcggct agacggaaag accccgtgaa 2820 cctttactgc agcttgacac tgaactttga ccttacttgt gtaggatagg tgggaggctt 2880 tgaagttgga acgctagttc caatggagcc gtccttgaaa taccaccctg gtaatgttga 2940 ggttctaact ctgtcccgtt atccgggacg aggaccgtgt ctggtgggta gtttgactgg 3000 ggcggtctcc tcctaaagag taacggagga gtacgaaggt gcgctcagcg tggtcggaaa 3060 tcacgcgtag agtataaagg caaaagcgcg cttaactgcg agacccacaa gtcgagcagg 3120 tacgaaagta ggtcttagtg atccggtggt tctgtatgga agggccatcg ctcaacggat 3180 aaaaggtact ctggggataa caggctgata ccgcccaaga gttcatatcg acggcggtgt 3240 ttggcacctc gatgtcggct catctcatcc tggggctgaa gcaggtccca agggtatggc 3300 tgttcgccat ttaaagaggt acgcgagctg ggtttagaac gtcgtgagac agttcggtcc 3360 ctatctaccg tgggcgctgg aaatttgaga ggatctgctc ctagtacgag aggaccagag 3420 tggacgaacc tctggtgtac cggttgtgac gccagtcgca tcgccgggta gctatgttcg 3480 gaagggataa ccgctgaaag tttttaagca 3510 <210> 2 <211> 2940 <212> DNA <213> Anaerobiospirillum succiniciproducens <220> <221> rRNA (222) (1) .. (2940) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 2 cttccctcag tgattcaaga cagcgcagca tgtgttttgt tcttgattca ttgcagttaa 60 aaagttcttt aaaaatttat cagacaagct tatttaaagt aaaaactcaa ttagcaacta 120 tttaatgggt aaaaagacca aagtcttgta aacctttcgg cagttgttaa aagttacttg 180 atccaatcat gatcaaggcc ttacgacctt tcggggttgt atggtcaagc atgaaagcgc 240 acatggtgga tgccatggcg tcaggaggcg atgaaggacg tgccaatctg cgataagcct 300 aggtaagccg ataaggggcg cttgaaccta ggatttccga atggggaaac ccgacactta 360 gtgtcatcta tgctgaagtt atagaagcga accaggggaa gtgaaacatc tcagtaccct 420 gaggaaaaga aatcaaatca gagattccct cagtagcggc gagcgaacgg ggaacagccc 480 aaaagttgat atgttctagt agaagcagtt gggaaactgc agggcacagg gtgatactcc 540 cgtatacgaa ggagcatatt tgatgagcgt gagtagggcg ggacacgtga tatcctgtct 600 gaatatgggg ggaccatcct ccaaggctaa atactacctg acgaccgata gtgaaccagt 660 accgtgaggg aaaggcgaaa agaaccccgg gaggggagtg aaaaagaacc tgaaaccgtg 720 tgcgtacaag cagtgggagc ctacttgtta ggtgactgcg taccttttgt ataatgggtc 780 agcgagttat ctgtattagc aggttaacct atatggggga gccgtagaga aatcgagtcc 840 taaccggcga atagttgata caggtagacc cgaaactggg tgagctagtc ctggccaggt 900 tgaaagcagg gtaacacctg ccggaggacc gaacccacta acgttgcaaa gttaggggat 960 gagctgggac taggggtgaa aggccaatca aactcagtga tagcttggtt ctcctcgaaa 1020 gctatttagg tagcgttctg cgaggacgta tgggggtaga gcactgttat ggggaagggg 1080 ccccatccgg ggttgccaat ccattgcaaa ctttcgaata ccatacagtt ggaagcagag 1140 gacagacggt gggtgctaac gttcatcgtc aagagggaaa caacccggac cgccagctaa 1200 ggtccccaag ttctagttaa gtgggaaacg atgtgggaag gcatagacag ccaggatgtt 1260 ggcttagaag cagccatcat ttaaagaaag cgtaatagct cactggtcga gtcggcctgc 1320 gcggaagatg taacggggct aaactagaca ccgaagctgc ggattcacac gcaagtgtga 1380 gtggtagagg agcgttctgt aaactgatga aggtgaggcg ggagccgagc tggaggtaac 1440 agaagtgcga atgctgacgt aagtaacgat aaaacacgtg aaaagcgtgt tcgccgaaag 1500 accaagggtt cctgtccaac gttaatcggg gcagggtgag tcggtgccta aggcgaggct 1560 gaaaagcgta gtcgaaggga aacaggtcaa tattcctgta ctttacttaa gtgcgatgag 1620 atgacggaga agggaaagtc agccacttat tggattgtgg tgaaaggctg taggtagata 1680 ccggggttaa aagactggta tcagtgatct gagagctgag acgaagcacc attgtgtgaa 1740 gtgactcgtg cccatgcttc ctggaaaagt ctctaagctt cagcttaagt agagccgtac 1800 cccaaaccga cacaggtggt cgggtagagg ataccaaggc gcttgggaga actcaggtga 1860 aggaactagg caaaattgta ccgtaacttc gggataaggt acgctgttac cggtgagagg 1920 acttgctcct taagctggga acagccgcag tgaaatggtg gctgggactg tttaacaaaa 1980 acacagcact ctgcaaacct gaaaggggaa gtatagggtg tgacacctgc ccggtgctgg 2040 aagattaatt gatggggtgc aagctcctga tcgaagtccc agtaaacggc ggccgtaact 2100 ataacggtcc taaggtagcg aaattccttg tcgggcaagt tccgacctgc acgaatggca 2160 taatgatggc cacactgtct cctcctgaga ctcagtgaag ttgaaatgtt tgtgatgatg 2220 caatctcccc gcggctagac ggaaagaccc catgaacctt tactgcagct ttgcattgga 2280 ctttgaaccg atctgtgtag gataggtggg aggcagtgaa gctaggacgc cagtcttagc 2340 ggagccaacc ttgaaatacc accctgattt gtttgaggtt ctaacctagg agcgtaatcc 2400 gcttcgggga ccgtgcatgg taggcagttt gactggggcg gtctcctcct aaagggtaac 2460 ggaggagtgc gaaggtacgc taggtacggt cggaaatcgt gctgatagtg caatggcaaa 2520 agcgtgcttg actgcgagac cgacaagtcg agcagatacg aaagtaggtc atagtgatcc 2580 ggtggctctg catggaaggg ccatcgctca acggataaaa ggtactctgg ggataacagg 2640 ctgataccgc ccaagagttc atatcgacgg cggtgtttgg cacctcgatg tcggctcatc 2700 tcatcctggg gctgtagccg gtcccaaggg tatggctgtt cgccatttaa agaggtacgt 2760 gagctgggtt taaaacgtcg tgagacagtt ttgtccctat ctgccgtggg cgttggaaga 2820 ttgacggggg ctgctcctag tacgagagga ccggagtgga ctcacctctg gtgtaccggt 2880 tgtcacgcca gtggcatcgc cgggtagcta tgtggggaaa ggataaccgc tgaaattttt 2940 2940 <210> 3 <211> 3379 <212> DNA <213> Bacteroides fragilis <220> <221> rRNA (222) (1) .. (3379) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 3 gctctgggag ccgggggtac ctgaagtacg taaccgcaag gatcgtccta gggtaaaact 60 ggtgactggg gctaagtcgt aacaaggtag ccgtaccgga aggtgcggct ggaacacctc 120 ctttctggag cgatgttgaa aacgacgttc attggttcta aattgtacta ctggtacttg 180 tttatttata gaatatagat caaccatcta tagcaatata gatagagata aacaagagaa 240 aaacagaagc cgagtctaaa acaggacacg gttgaactag tcctatagct cagttggtta 300 gagcgctaca ctgataatgt agaggtcggc agttcaactc tgcctgggac taccaacaga 360 tagatatttt atcttgtatg attgggggat tagctcagct ggctagagca tctgccttgc 420 acgcagaggg tcaacggttc gaaatccgtt attctccact ccgataccgc gacctaacag 480 gcttgcgtgt aatcaaacga tctttgacat gatgtacaga aaaaagtaaa tttagtaaga 540 gctaaaagta tatatcgaac catttggttc agatcctttt taagaaaagg agatgaatct 600 ggcgataaaa gaaagtaaat aagggcgcat ggcggatgcc ttggctctcg gaggcgatga 660 angacgtgat aagctgcgat nagcttcggg taggtgcaaa tgacctttga ttccgaagat 720 ttccgaatgg gacaaccccg gcattctgaa ggaatgtcat ccaacttggt tggaggctaa 780 cgcagggaac tgaaacatct tagtacctgc aggaaaagaa aataaataat gattccccta 840 gtagtggcga gcgaacgggg attagcccaa accacccatg ttacggcatg tgtggggttg 900 taggaccacg ctctcgcaag acagttattg agaagaaacg actggaaagt cgtgccatag 960 acggtgatag cccggtattc taaggtaacc gaagcgtagt ggtatcctga gtagcgcgga 1020 gcacgaggaa ttctgcgtga accagccggg accatccggt aaggctaaat actcccgaga 1080 gaccgatagc gaaccagtac tgtgaaggaa aggtgaaaag cacttcgaat agaagagtga 1140 aatagtccct gaaaccatgc gcctacaagc ggtcggagca gcttaagctg tgacggcgtg 1200 ccttttgcat aatgaaccta cgagttactt tttccggcaa ggttaagcat cttgagatgt 1260 ggatccgaag cgaaagcgag tctgaacagg gcggatagtc ggaaggagta gacgcgaaac 1320 caagtgatct acccttgggc aggttgaagg ttaggtaaca ctaactggag gaccgaaccg 1380 ataagcgttg aaaagcttcc ggatgacctg agggtggggg gtgaaaggct aatcaaactt 1440 ggagatagct cgtactcccc gaaatgcatt taggtgcagc cttgagagtt actaatgtga 1500 ggtagagcga ctgataagat gcgagggctt caccgcctat caagtcttga taaactccga 1560 atgcgcatta gttctatctc aggagtgagg gcatgggtgt aaggtccatg tctaaagaga 1620 agaatccaga ccatcagtaa ggtccccaaa taaacattaa gttgacctaa cgaagtcaga 1680 ttgctaagac agctaggatg ttggcttgga agcagccatt catttaaaga gtgcgtaaca 1740 gctcactagt cgaggagttt ggcgtggata ataatcgggc ataagtgttt taccgaagct 1800 atgggatccg tatggatcgg taggggagca ttccactcag cgttgaaggc gaagcgtgag 1860 ctttactgga gcgtgtggaa aagcaaatgt aggtataagt aacgataaag ggggtgagaa 1920 accccctcgc cgcaagacta aggtttcctg atcaacgcta atcggatcag ggttagtcgg 1980 gtcctaaggc tcagccgaac ggtgaggccg atggcagaac aggttaatat tcctgtacta 2040 cctataagag tgatgtggag acggaggagt gacaacgccg cggactgacg gaatagtccg 2100 ttaaagggtg tagatgttga ttatcccagg caaatccggg ataagagtcg aacctgacag 2160 tataccaatt tcctcggaaa acggtaatag tgcgtgtaaa catactccca agaaaatccg 2220 ctaaacttaa tcttataggt acccgtaccg taaacggaca cacgtagtcg ggttgaatat 2280 actcaggcgc ttgagtgaat cacggttaag gaactaggca aattgaccct gtaacttcgg 2340 gataaagggt ccctacccgg tgacggggag ggcgcagaaa aataggtcca ggccaacctg 2400 tttaacaaaa acacagggct gtgcaaaatt gaaagatcac gtttacagcc ctgacacctg 2460 cccggtgctg gaaggttaag aggagatgtc atcgcaagag aagcattgaa ttgaagcccc 2520 agtaaacggc ggccgtaact ataacggtcc taaggtagcg aaattccttg tcgggtaagt 2580 tccgacctgc acgaatggcg taacgatggc cacactgtct ccaccagaga ctcagcgaag 2640 ttgaaatgtt tgtaatgatg catctccccg cggaaagacg gaaagacccc atgaaccttt 2700 actgtagctt tgtattggat tttgaacgga tctgtgtagg ataggtggga ggctttgaag 2760 tgaggacgct agttctcatg gagccgacgt tgaaatacca ccctggtgcg ttgaggttct 2820 aacccaggtc ccttatcggg atcggggaca gtgcatggta ggcagtttga ctggggcggt 2880 ctcctcccaa agcgtaacgg aggagttcga aggtacgcta gttacggtgc ggacatcgtg 2940 acgatagtgc aatggcataa gcgtgcttaa ctgcgagact gacaagtcga gcagatgcga 3000 aagcaggaca tagtgatccg gtggttctgt atggaagggc catcgctcaa cggataaaag 3060 gtactctggg gataacaggc tgataccgcc caagagttca tatcgacggc ggtgtttggc 3120 acctcgatgt cggctcatct catcctgggg ctgtagtcgg tcccaagggt atggctgttc 3180 gccatttaaa gaggtacgtg agctgggttt aaaacgtcgt gagacagttt ggtccctatc 3240 ttccgtgggc gctgcagatt tgaggaagcc tgctcctagt acgagaggac cggagtggac 3300 acacctctgg tgtatcggtt gtcacgccag tggcattgcc gagtagctaa gtgtggaaga 3360 gataaccgct gaacttttt 3379 <210> 4 <211> 3138 <212> DNA <213> Cardiobacterium hominis <220> <221> rRNA (222) (1) .. (3138) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 4 cccctgataa gggtgaggtc aaagttcaat ttttcagacc ccccaaaaag ggccatagct 60 cagctggaga gcgcctgctt ttgcacgcag agtcagagtt cgatcctcct gggctcccca 120 tttgaaacgt agtttgctaa agagagcaga aaattgaaca tgaagctaaa gctgatgtaa 180 gaacacagat ggagcacatg gtgttcaatt ttttgttgtc ttaggacaat gatctataac 240 aaccggaaac attcaattta aacaactaaa ttgagtagct gagaaaaaca agacgttgtt 300 acttatgttt tcctctgcgt agccaacaga tgatgagaga gcaaggcgaa agcgaagcat 360 acttcagaat ctgaaaacgt agagtagtaa agagagaaca gcacaaccga aagataactt 420 gaggttgtat gatcaagtga ataagtgcac acggtggatg ccttggcgac aacaggcgaa 480 gaaggacgtg gaaatctgcg aaaagccccg ggaagctgat aacaagcaat gatccgggga 540 tctccgaatg gggcaacccg accttagggt catccgtagt tgaatacata gactgcggaa 600 gcgaaccggg agaactgaaa catctaagta ccccgaggaa aagaaatcaa ccgagattcc 660 gcaagtagcg gcgagcgaac gcggagaagc ccaattaagt tgcgtatgag ctagtagaac 720 atcctgggaa gggtggccat agagggtgat agccccgtat acgaaagcca tacacaatga 780 atcgagtagg gcgggacacg tgaaatcctg tctgaagatg gggggaccat cctccaaggc 840 taaatactcg ttgtcgaccg atagtgaacc agtaccgtga gggaaaggcg aaaagaaccc 900 tggtgaaggg agtgaaatag aacctgaaac cgtgtgcata caagcagtca gagcatatga 960 agatatgtga tggcgtacct tttgtataat gggtcagcga gttacattca gtggcgaggt 1020 taaccgaata ggggagccgt agcgaaagcg agtcttaata gggcgataag tctctgggtg 1080 tagacccgaa accgggcgat ctatccatgg gcaggttgaa ggttgagtaa catcaactgg 1140 aggaccgaac ccacgcatgt tgaaaaatgc ggggatgacc tgtggatagg agtgaaaggc 1200 taaacaagct cggagatagc tggttctccc cgaaaactat tgaggtagtg ccttgtagat 1260 tgacttacgg gggtagagca ctgtttcggc tagggggtca taccgactta ccaacccgat 1320 gcaaactcga ataccgtaaa gttttactac aggagacaca cggcgggtgc taacgtccgt 1380 cgtgaagagg gaaacaaccc agaccgccag ctaaggtccc gaaatgacag ttcagtggga 1440 aacgaagtgg gaaggcttag acagctagga ggttggctta gaagcagcca tcctttaaag 1500 aaagcgtaat agctcactag tcgagtcgtc ctgcgcggaa gatttaccgg ggctaaactg 1560 tctaccgaag ctgcggattt gcatgaagat gcaagtggta ggggagcgtt gtgtaagccg 1620 aagaaggtgt gttgagaagc atgctggagg tatcacaagt gcgaatgctg acatgagtaa 1680 cgataatgcg ggtgaaaaac ccgcacaccg aaaacccaag gtttcctgcg caacgctaat 1740 cggcgcaggg tgagtcggcc cctaaggcga ggcggaaacg cgtagtcgat gggaaacagg 1800 ttaatattcc tgtactgatc ataattgcga tggggggacg gagaaagcta ggccagcaca 1860 ctgttggatg tgtgtttaag cgcgtaggtt ggggacttag gcaaatccgg gttcctaaga 1920 ctgaaacgtg atgacgagtt tctacggaaa cgaagtggtt gatgctctgc ttccaggaaa 1980 agcctctaag cttcagatta tgacaaccgt accccaaacc gacacaggtg ggtaggaaga 2040 gaattccaag gtgcttgaga gaactcgggt aaaggaactc ggcaaaatga taccgtaact 2100 tcgggataag gtatgcctct gatggtgaat agctgttgga ggccgcagag aataggccgc 2160 tgcgactgtt tatcaaaaac acagcactct gcaaacacgc aagtggacgt atagggtgtg 2220 acgcctgccc ggtgccggaa ggttaattga tggggtgcaa gctcttgatc gaagccccgg 2280 taaacggcgg ccgtaactat aacggtccta aggtagcgaa attccttgtc gggtaagttc 2340 cgacctgcac gaatggcgta acgatggcgg cgctgtctct acccgagact cagtgaagtt 2400 gaaatcgctg tgaagatgca gtgtacccgc ggcaagacgg aaagaccccg tgaaccttca 2460 ctacagcttg acactgaaca ttgaaataag ttgcatagga taggtgggag gctttgaagc 2520 agagactctg gtttctgtgg agccgacctt gaaataccac cctgcttatt ttgatgttct 2580 aacgtagacc cgtaatccgg gttgcagaca gtgtctggtg ggtagtttga ctggggcggt 2640 ctcctcccaa agagtaacgg aggagtgcga aggtgggctc ggtgcggtcg gaaatcgcac 2700 caagagtgca aaggcagaag cccgcttaac tgcgagacag acaagtcgag cagatacgaa 2760 agtaggtctt agtgatccgg tggttctgaa tggaagggcc atcgctcaac ggataaaagg 2820 tactccgggg ataacaggct gattcctccc aagagtccat atcgacggag gagtttggca 2880 cctcgatgtc ggctcatcac atcctggggc tgtagcaggt cccaagggta tggctgttcg 2940 ccatttaaag tggtacgcga gctgggttca gaacgtcgtg agacagttcg gtccctatct 3000 gccgtgggcg ttggagattt gagggaagct gttcttagta cgagaggacc ggaatggacg 3060 aacctctggt gtaccggttg tcacgccagt ggcacagccg ggtagctatg ttcggaaggg 3120 ataaccgctg aacttttt 3138 <210> 5 <211> 3332 <212> DNA <213> Chryseobacterium meningosepticum <220> <221> rRNA (222) (1) .. (3332) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 5 ctctggagct gggggtacct gagtcggtga ccgtaaagga gctgcctagg gtaaaactag 60 taactagggc taagtcgtaa caaggtagcc gtaccggaag gtgcggctgg aacatctcat 120 tttagagcgt cctttggacg ataaacaaaa caaagatcac ttaggtgatc acttacttaa 180 acaaagcaca gctttggttt tactttggtt gctattacaa aagataaccc cttagattag 240 taacagggaa agagatagtt tataattatg aattttaatg aataattata aattaatgaa 300 gtctcgtagc tcagctggtt agagcgctac actgataatg tagaggtcgg cagttcgagc 360 ctgcccgaga ctactaattt aaaagatttg aagttagaga ttggaagttt gaagagataa 420 tttcaaacct caaactaaga atttcaaatt taagaggggg aattagctca gctggctaga 480 gcgcctgcct tgcacgcagg aggtcaaggg ttcgactccc ttattctcca cagttttaga 540 agagagacac aagtatacga atagagccaa taacaatatt cgtttgttga tcagaagaaa 600 agaacaaaag atcattgaca ttaacggtaa agacatcaca aagagataac cgagcactcc 660 ttgagtgcag agtaaaaaat aaattaatta ttaggaaaga aatcgttaag ggcgtatggc 720 ggatgcctag gctttcagag gcgacgaagg acgtggtaag ctgcgaaaag ctacggggat 780 tggcacacac gaattgatcc gtagatatcc gaatggggca acccggcata ttgaagatat 840 gtcacccagc aatgggagca aacccggaga actgaaacat ctaagtaccc ggaggaaaag 900 aaatcgaaga gattccgtaa gtagtggcga gcgaaagcgg attagcccaa aagtctttat 960 atgtttagaa gaacacactg gaaagcgtgg ccatagaggg tgatagcccc gtatttgaaa 1020 ggcatattta gatgataaat gagtagggcg ggacacgtga aatcctgtct gaatatgggg 1080 ggaccatcct ccaaggctaa atactcctga aagaccgata gtgaacaagt actgtgaagg 1140 aaaggtgaaa agcacttcga atagaagggt gaaatagaac ctgaaaccgt acgcctacaa 1200 gcggtcggag cagctataag ctgtgacggc gtgccttttg cataatgagc ctacgagtta 1260 atcttactag cgaggttaag gacttaaggt ccggagccgt agcgaaagcg agtctgaata 1320 gggcggatag ttagtgggat tagacgcgaa accttgtgat ctacccatgg gcaggttgaa 1380 gctttggtaa cacaaagtgg aggaccgaac cggttgacgt tgaaaagtct tcggatgacc 1440 tgtgggtagg ggtgaaaggc caatcaaact gggagatagc tcgtactccc cgaaatgcat 1500 ttaggtgcag cgtcgattta gtttattaga ggtagagcta ctgattggat gcgggggttt 1560 catcgcctac caattcctga caaactccga atgctaataa atgtaacttg ggagtcagaa 1620 catgggtgat aaggtccgtg ttcgaaaggg aaacagccca gaccaccagc taaggtccca 1680 aaatatatgt taagtggaaa aggatgtggc gttgcccaga caactaggat gttggcttag 1740 aagcagccat catttaaaga gtgcgtaata gctcactagt cgagtgatcc ggcatggata 1800 ataatcgggc ataagcatat taccgaagct atggcagtat ttatactggg taggggagca 1860 ttctatttgc gccgaagcag tactgtgagg tattgtggag cggatagaaa agaaaatgta 1920 ggcataagta acgataaagg gggcgagaaa ccccctcacc gaaagactaa ggtttcctca 1980 gccatgctaa tcagctgagg gttagtcggg gcctaacgcg aagccgaatg gcgtagtgga 2040 tggacaacgg gttaatattc ccgtaccagt atattaataa aagtgacgga gttggaaact 2100 aggtgcgtac tgacggaata gtacgttgaa gtaatcgtga ggttacgata gtacagcaaa 2160 tcttcggatg cgctgataat cctggggacc cgcttccaag aaaagcgaaa tatactgccc 2220 gtaccaaaac cgacacaggt agtcgaggag agaatcctaa ggtgctcgag tgagtcgtgg 2280 ctaaggaact aggcaaaata gtctcgtaac ttcggaagaa gagacgcctc cctccgggga 2340 ggccgcagtg aagaggccca ggcgactgtt tatcaaaaac acaggactct gctaaatcga 2400 aagatgctgt atagggtctg acacctgccc ggtgctggaa ggttaaggaa ggtgcttaga 2460 gttaaatcga aggcattaac tgaagcccca gtaaacggcg gccgtaacta taacggtcct 2520 aaggtagcga aattccttgt cgggtaagtt ccgacctgca cgaatggtgt aacgatgctg 2580 ggcactgtct cagccacgag ctcggtgaaa ttgtagtatc ggtaaagatg ccgattaccc 2640 gcaatgggac gaaaagaccc tgtgaccctt tactataact tcgtattgac ttcgagtaaa 2700 caatgtgtag gataggtgga ggctatgaag caggcacgct agtgtttgtg agccaacgtt 2760 gaaataccac cctttgttta cttggagcct aacttctacg gaaggacatt gcgtggtggg 2820 tagtttgact ggggtggtcg cctccaaaag agtaacggag gctttcaaag gtaccctcag 2880 cacgcttggt aaccgtgcgt agagtgtaat ggcataaggg tgcttgactg tgagacctac 2940 aagtcgatca ggtgcgaaag caggacatag tgatccggtg gttccgtatg gaagggccat 3000 cgctcatagg ataaaaggta ctccggggat aacaggctag tctcccccaa gagctcacat 3060 cgacggggag gttcggcacc tcgatgtcgg ctcgtcacat cctggggctg gagaaggtcc 3120 caagggttgg gctgttcgcc cattaaagtg gcacgcgagc tgggttcaga acgtcgtgag 3180 acagttcggt ctctatctat tgcgggcgtt agatgtttga gagggcttga ttctagtacg 3240 agaggaccga attgaacaaa cctctggtgt atcagttgta ccgccaggtg caccgctaaa 3300 tagctatgtt tggaagagat aagcgctgaa ag 3332 <210> 6 <211> 3252 <212> DNA <213> Clostridium ramosum <220> <221> rRNA (222) (1) .. (3252) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 6 gcaccgctat agtttgtaac acccgaagtc ggtgaggtaa ccttttggag ccagccgccg 60 aaggtgggaa agaactgatt ggggatgata ttgtctttgt aacaagggta gccgtatccg 120 gaaggtgcgg ctggatcacc tcctttctaa ggaaattata cggaatgatc gaagacctga 180 tgcggtctta tagacaggtg cgtttngtag atacttgttt agttttgaag tgatcattcc 240 ttcaaagcgt atgactcaga gatcattgat tactggataa gtagacaatc acattgcaat 300 agaaatagca aaatnagatc gatcatagta attgtctata ttgacgggtt acgttactag 360 aaaacgcact gaaactaagt attttctcct aaaacataaa tcgaacggat ggatggattt 420 tagtaaatag ttgatgatag taatcaagca acaccatata ggtaagtaag aaaaggtatg 480 gcggatgcct ggcacagaga gcgaagaagg acgcagcaac agcaaatgtg acggttagca 540 gtgagcatgg cttatctgtg gatgtctgaa taggggaacc cccctggagt agtagtccag 600 cgtatcctca agtgaatata tagcttcaga gaggcgagac tcaggggaac ctgaaacaat 660 ctaaggtcct caggaaggag aggagaaaat aacacgaatg attccctaag tatcggcgag 720 cgaaagggga agagcccaaa ccgatcttag gatggggttg taggactgtc ggcaaagagc 780 aagaaatcat tataggcgaa cggaatggga agtccggcga aacagggtga cagccccgta 840 gccgaaatag tgaagaagca cgagacagca cctgagtacg gcgggacacg aggaatccct 900 ccttgtcgtg aatccaccag gaccttctgg taaggctaaa tactaccttc tgtgaccgta 960 tagtgaacca ataccgtgag ggaaaggtga aatagacccc cgggaagggg agtgaaatag 1020 atcctgaaac cgtatgctta caagaagtta gagcccgtta aagggtgata gcgtgccctt 1080 ttgtagaatg aaccggcgag ttacgatatg gagcgaggtt aagcaggaat atgccggagc 1140 cgaagcgaaa gcgagtttta acaggggcga aagttgcatg tcgtagaccc gaaaccgagt 1200 gatctagcca tgaccaggtt gaagttgggg taaaacccga tggaggaccg aaccgacccc 1260 ccgttgaaac gttgtgcgga tgatgttggt ggctagtggg tgaaattcca atcgtaactc 1320 ggagatagct tggttctccc cgaaatagct ttagggctag cagtcgtagt gtaaagtcag 1380 tgtgaaggta gagcactgta atatgtgatg gccccatctc ggggtactga acataatcaa 1440 actctcgaat gtgcacaaag atatactcgg cagtcagaca gtgggtgata aggtccattg 1500 tcaaaaaggg aaacagccca gaccatcagc taaggtccca aaatatatac taagtggaaa 1560 aggatgtgga gatgtccaga caactaggca ggttggctca gaatgcagcc attcaacctt 1620 taatagagtg cgtaacagct cactagtcga agtgactctg cgccgataat ttatccgggg 1680 ctaagtatat ttaccgaagc tatggattta cgcgttaaag cgtgagtggt tagggggagc 1740 gttctatgtg cggagaagcg gtaccgtaag gagccgtgga gcgcatagaa gagagaatgc 1800 cggtgtgagt agcgaaacgt gggtgagaat cccacgccac cgaaaaccca aggtttccag 1860 aggaaggttc gtccgcctca gggtaagtcg ggacctaagg cgaggccgag aggcgtagtc 1920 gatggacaac aggtagagat tcctgtactt acggtatgaa tgatggagtg acggagaagg 1980 ctagcggatc ctgctgatgg aaatgcaggt gcaagcgagg gagccgccag ccaggcaaat 2040 ccggctgtcg aaaggccatg gcgctgaggc gtatggaaag ctgcggcaag tacagaagtc 2100 cgtgaagcca gcttccaaga aaagcttcta gtgataatca tacagtaacc cgtaccgaaa 2160 atggacacac atgggtgagg agagaatacc taaggtgagc gagagaacta tagctaagga 2220 actctgcaaa atgactccgt aacttaggga taaggagtgc tcatagagat atgagccgca 2280 gtgaaacggc ccaagcgact gtttaccaaa aacacagctc tatgctaagt cgaaagacga 2340 cgtatatggg gtgacgcctg cccggtgctg gaaggttaag aggatgtgtc agcgcaagcg 2400 aagcattgaa ttgaagcccc agtaaacggc ggccgtaact ataacggtct aagagggaat 2460 tcctcaggta agttaccgcc ccaaagagag cgatttgtgg tgttcatctg tagatggtga 2520 agtattagta cctgtcaaga gcaggttacc ccccctagac ggaaagaccc catggagctt 2580 tactgtagct tgatattgga ttctttgatg caaagatgta caggcatagg taggagtatg 2640 agagacatgc acgccagtgt gtgaggagtc aatgttggga tactactctt ccttgtattg 2700 gagttgctaa ccggatgcca tggaactggc aacgggcaca gtagtcaggt cgggcagttt 2760 gactggggcg gtcgcctccc aaagagtaac ggaggcgccc aaagataccc tcagcttgga 2820 tggaaatcaa gcgcagagtg caaaggcata agggtgtttg actgcgagac ctacaagtcg 2880 agcagggacg aaagtcgggc ttagtgatcc ggcggtgctg aatggaaagg ccgtcgctca 2940 acggataaaa gctaccctgg ggataacagg ctgatctccc ccaagagttc acatcgacgg 3000 ggaggtttgg cacctcgatg tcggctcatc gcatcctgga gctgaagtcg gttccaaggg 3060 ttgggctgtt cgcccattaa agcggtacgc gagctgggtt cagaacgtcg tgagacagtt 3120 cggtccctat ctgtcgtggg cgtaggaagt ttgagaagat ctgtcctcag tacgagagga 3180 ccgggaggac atatcaatgg tgcaccagtt gtcacgccag ggcacagcgg tagctaaata 3240 ggaagggaaa aa 3252 <210> 7 <211> 3531 <212> DNA <213> Comamonas acidovorans <220> <221> rRNA (222) (1) .. (3531) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 7 ctcatgggag cgggtctcgc cgagtaggta gcctaaccgc aaggagggcg cttaccacgg 60 cggggttcgt gactggggtg aagtcgtaac aaggtagccg tatcggaagg tgcggctgga 120 tcacctcctt tctggaaaac tgctgttcaa gttgaacgcc cacacttatc ggttgttgga 180 acaagccatg tgccctggtt gcagggtggg tggactgggt ctgtagctca gctggttaga 240 gcaccgtctt gataaggcgg gggtcgttgg ttcgagccca actagaccca ccaagattcc 300 aatatctggt tgtcgaggat cccgggggat tagctcagct gggagagcac ctgctttgca 360 agcagggggt cgtcggttcg atcccgtcat cctccaccaa gatcgcgctg gtggcagcag 420 cttgaaaaag cagctggagg aagcgaaaag agcacgaaaa aagcgtgcta taatatttga 480 ctcaacacta aagcagtctc gtgtgagact gctttagtgt tgatagttat ctaactatca 540 atcggctgtt ctttaaaaat tcatagagtc gaaatcagcg ttgctgacgg aaagagattt 600 caatctcacc gtgccgtcag caacattttg attgcgtcaa aacgaatgaa actttgtttt 660 attcaagtaa tgacgaattg ttctcttgac agaaatgtca aagaattcat tcacattacg 720 gcataacgcg cgaggtgaga gacctcgcaa gtccttgaaa gaaaacggcg aaatctcgca 780 agagagatca aagttatagg gtcaagtgac taagagcatg tggtggatgc cttggcgatg 840 ataggcgacg aaagacgtga tagcctgcga taagcttcgg ggagctggca aattagcttt 900 gatccggaga tttctgaatg gggaaaccca cccgcaaggg tatcgcatac tgaatacata 960 ggtatgcgag gcgaacctgg agaactgaaa catctaagta cccagaggaa aagacatcaa 1020 ccgagattcc gaaagtagtg gcgagcgaaa tcggaagagc cttctagtga tagtcagacg 1080 gttaacaaaa cggaatggaa agtccggcca tagtaggtga tagccctgta tgtgaaaacc 1140 gactggtggt actgagctag agaaaagtag ggcggggcac gagaaaccct gtctgaatat 1200 ggggggacca tcctccaagg ctaaatactc atcatcgacc gatagtgaac cagtaccgtg 1260 agggaaaggc gaaaagaacc ccgggagggg agtgaaatag atcctgaaac cgcatgctta 1320 caaaaagtag gagcccgcaa gggtgactgc gtaccttttg tataatgggt cagcgactta 1380 cattcagtgg caaggttaac cgaatagggg agccgtagag aaatcgagtc cgaatagggc 1440 gtccagtcgc tgggtgtaga cccgaaacca agtgatctat ccatggccag gatgaaggtg 1500 ccgtaacagg tactggaggt ccgaaccgac tagtgttgca aaactagcgg atgagctgtg 1560 gataggggtg aaaggctaaa caaacttgga aatagctggt tctctccgaa aactatttag 1620 gtagtgcctc aagtattacc tgcgggggta gagcactgtt taggctaggg ggtcatggcg 1680 acttaccaaa cctatgcaaa ctccgaatac cgcagagtac agcttgggag acagagcacc 1740 gggtgctaac gtccggactc aagagggaaa caacccagac cgccagctaa ggtccctaaa 1800 attggctaag tgggaaacga agtgggaagg ctaaaacagt caggatgttg gcttagaagc 1860 agccatcatt taaagaaagc gtaatagctc actgatcgag tcgtcctgcg cggaagatgt 1920 aacggggcta agccagttac cgaagctgcg gatttgcaat ttattgcaag tggtaggaga 1980 gcgttctgta agcctgtgaa ggtgcctggt aacgggtgct ggaggtatca gaagtgcgaa 2040 tgctgacatg agtagcgtta aagggggtga aaagccccct cgccgtaagc gcaaggtttc 2100 ctacgcaacg ttcatcggcg tagggtgagt cggcccctaa ggcgaggcag agatgcgtag 2160 ctgatgggaa acaggtcaat attcctgtac cgatgtgtag tgcgatgtgg ggacggagaa 2220 ggttagctca gccaactgtt ggatatgttg gttcaagcct gtagtcgtgc ctggtaggca 2280 aatccgccgg gcttagatga ggggtgataa cgagtctgct tgcagacgaa gtgagtgata 2340 ccctgcttcc aggaaaagcc actaagcttc agctacacac gaccgtaccg caaaccgaca 2400 ctggtgcgcg agatgagtat tctaaggcgc ttgagagaac tctggagaag gaactcggca 2460 aattgatacc gtaacttcgg gagaaggtat gccgcaagta ggtgaacttg aacaaaggga 2520 gcccaaagcg gttgcaaaaa atcggtggct gcgactgttt aataaaaaca cagcactctg 2580 caaacacgaa agtggacgta tagggtgtga cgcctgcccg gtgctggaag attaaatgat 2640 ggggtgcaag ctcttgattg aagtcccagt aaacggcggc cgtaactata acggtcctaa 2700 ggtagcgaaa ttccttgtcg ggtaagttcc gacctgcacg aatggcgtaa cgatggccac 2760 actgtctcct ccagagactc agcgaagttg aaatgtttgt gatgatgcaa tctccccgcg 2820 gaaagacgga aagaccccat gaacctttac tgtagctttg tattggattt tgaacggatc 2880 tgtgtaggat aggtgggagg ctttgaagtg aggacgctag ttctcatgga gccgacgttg 2940 aaataccacc ctggtgcgtt tgaggttcta acccaggtcc cttatcggga tcggggacag 3000 tgcatggtag gcagtttgac tggggcggtc tcctcccaaa gcgtaacgga ggagttcgaa 3060 ggtacgctag ttacggtcgg acatcgtgac gatagtgcaa tggcataagc gtgcttaact 3120 gcgagactga caagtcgagc agatgcgaaa gcaggacata gtgatccggt ggttctgtat 3180 ggaagggcca tcgctcaacg gataaaaggt actctgggga taacaggctg ataccgccca 3240 agagttcata tcgacggcgg tgtttggcac ctcgatgtcg gctcatctca tcctggggct 3300 gtagtcggtc ccaagggtat ggctgttcgc catttaaaga ggtacgtgag ctgggtttaa 3360 aacgtcgtga gacagtttgg tccctatctt ccgtgggcgc tgcagatttg aggaagcctg 3420 ctcctagtac gagaggaccg gagtggacac acctctggtg tatcggttgt cacgccagtg 3480 gcattgccga gtagctaagt gtggaagaga taaccgctga aattttaacc a 3531 <210> 8 <211> 3200 <212> DNA <213> Corynebacterium diphtheriae <220> <221> rRNA (222) (1) .. (3200) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 8 acaccgcgcc gtcacaccac gcagcagttt gtaacacccc gagtcggtag ggtaaccctt 60 tatggagcca gccgccgaag gtgggacaga taattggggt gaagtcgtaa caaggtagct 120 gtatcggaag gtgcggctgg atcacctcct ttctaaggaa aaggaaacct gtgagttttc 180 gttcttctct atttgttcag ttttgagagg ttagtacttc tcagtatgtt tgttctttga 240 taactagata agaaagttag taaagttagc atagataatt tattatttat gacacaagta 300 accgagaatc atctgaaagt gaatctttca tctgtattgg aagtatcatc gctgatacgg 360 aaaatcagaa aaacaacctt tacttcgtag aagtaaattg gttaagttag aaagggcgca 420 cggtggatgc cttggcacta ggagccgaag aaggacggga ctaacaccga tatgctttgg 480 ggagctgtac gtaagcgttg atccagagat ttccgaatgg gggaacccac tatctttagt 540 cggatagtat ccttacgtga atacatagcg tgaggaaggc agacccaggg aactgaaaca 600 tctaagtacc tggagcaaga gaaagaaaaa tcgatttcct gagtagcggc gagcgaaacg 660 gaaagagccc aaaccaagaa gcttgcttct tggggttgta ggacacttct atacggagtt 720 acaaaagaaa gttataaatg aagcggtctg gaaaggcccg ccaaaagacg ggtaacagcc 780 cggtagttga aatggctttc cctccccaga gtggatcctg agtacggcgg aacacgtgaa 840 attccgtcgg aatccgggag gaccatcttc ccaaggctaa atactcccct agtgaccgta 900 tagtgaaccc agtaccgtga gggaaaggtg aaaagcaccc ccgcaagggg cagtgaaaca 960 gttcctgaaa ccgtgtgcct acaagtagtt agagcccgtt aatgggtgat agcgtgcctt 1020 ttgtagaaat gaaccggcga gttacgattt gttgcaaggt taagcggaaa aagcggagcc 1080 gtagcgaaag cgagtctgaa tagggctgca taagtaacag gtcgtagacc cgaaaccagg 1140 tgatctaccc atgtccagga tgaaggtaag gtaatactta ctggaggtcc gaacccacgc 1200 acgttgaaaa gtgcggggat gaggtgtggg tagcggagaa attccaatcg aacttggaga 1260 tagctggttc tctccgaaat agctttaggg ctagcctcga ggtaaagagt catggaggta 1320 gagcactgtt tggactaggg gcccttctcg ggttaccgaa ttcagataaa ctccgaatgc 1380 catgtactta tactcgggag tcagactgcg agtgataaga tccgtagtcg aaagggaaac 1440 agcccagacc accagttaag gtccccaaat atatgttaag tggaaaagga tgtggggttg 1500 cttagacaac caggatgttg gcttagaagc agccaccatt gaaaattgaa gtgcgtaata 1560 gctcactggt ccgagtgacc ccgcgccgaa aatgtaccgg ggctaaacat attaccgaaa 1620 ctgtggatga acctctttag aggttcgtgg taggagagcg ttctaagggc ggtgaagtca 1680 gaccggaagg actggtggag cgcttagaag tgagaatgcc ggtatgagta gcgaaagaag 1740 ggtgagaatc ccttccaccg aatatctaag gtttcctgag gaaggctcgt ccgctcaggg 1800 ttagtcggga cctaagccga ggccgatagg cgtaggcgat ggacaacagg tagagattcc 1860 tgtaccagtg ctaattgttt aaccgatggg gtgacacaga aggataggga atcgcacgaa 1920 tggaaatgtg cgtccaagca gtgagtgtga gaagtaggca aatccgcttc tcgcgaagca 1980 tgagctgtga tggggaagga aattaagtac ggaagttcct gatttcacgc tgtcaagaaa 2040 agcctctagg aagagtagta ctgcccgtac cgcaaaccga cacaggtaga tgaggagaga 2100 atacctaagg tgagcgagag aacaatctcg ttaaggaact cggcaaaatg accccgtaac 2160 ttcgggagaa ggggtgctct attagggtgc aagcccgaga gagccgcagt gaataggccc 2220 aggcgactgt ttagcaaaaa cacaggtctc tgcaaaaccg taaggtgacg tataggggct 2280 gacgcctgcc cggtgctgga aggttaagag gagtgcttag cttcggcgaa ggtacgaatt 2340 gaagccccag taaacggcga gaccgtaact ataacgggtc ctaaaaggta gcgaaattcc 2400 ttgtcgggta agttccgacc cgcacgaaag gcgcaacgat ctgggcactg tctcaacgag 2460 agactcggtg aaattatagt acctgtgaag atgcaggtta cccgcgacag gacggaaaga 2520 ccccgtggag ctttactgca acctgatatg gaatgtttgt accgcttgta caggataggt 2580 aggagccgaa gagacgtgtg cgctagcata cgaggaggca atggtgggat actaccctgg 2640 ctgtatgacc attctaaccc gccacgctta ggcgcgtggg gtagacaagt gtcaggtggg 2700 cagtttgact ggggcggtcg cctcctaaag agtaacggag gcgcccaaag gttccctcag 2760 aatggatgga aatcattcgc agagtgtaaa ggcacaaggg agcttgactg cgagactgac 2820 aagtcgagca gggacgaaag tcgggcttag tgatccggtg gttccgcatg gaagggccat 2880 cgctcaacgg ataaaagcta ccccggggat aacaggctta tctcccccaa gagtccacat 2940 cgacggggag gtttggcacc tcgatgtcgg ctcgtcgcat cctggggctg tagtcggtcc 3000 caagggttgg gctgttcgcc cattaaagcg gcacgcgagc tgggttcaga acgtcgtgag 3060 acagttcggt ccctatccgt cgcgggcgca ggaaatttga gaggagctgt ccttagtacg 3120 agaggaccgg gatggacaca ccgctggtgt accagttgtt ccgccaggag catcgctggt 3180 agctatgtgg gcagggaaaa 3200 <210> 9 <211> 1830 <212> DNA <213> Klebsiella oxytoca <220> <221> rRNA (222) (1) .. (1830) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 9 gaatgttatc acggagacac acggcgggtg ctaacgtccg tcgtgaagag ggaaacaacc 60 cagaccgcca agctaaggtc ccaaagtcat ggttaagtgg gaaacgatgt gggaaggccc 120 agacagccag gatgttggct tagaagcagc catcatttaa agaaagcgta atagctcact 180 ggtcgagtcg gcctgcgcgg aagatgtaac ggggctaaac catgcaccga agctgcggca 240 gcgacactat gtgttgttgg gtaggggagc gttctgtaag ccgttgaagg tggcctgtga 300 gggttgctgg aggtatcaga agtgcgaatg ctgacataag taacgataat gcgggtgaaa 360 aacccgcacg ccggaagacc aagggttcct gtccaacgtt aatcggggca gggtgagtcg 420 acccctaagg cgaggccgaa aggcgtagtc gatgggaaac aggttaatat tcctgtactc 480 ggtgttactg cgaagggggg acggagaagg ctatgttggc cgggcgacgg ttgtcccggt 540 ttaagcatgt aggcggatgt tccaggtaaa tccggaacgt tactaacgct gaggtgtgat 600 gacgaggcac tacggtgctg aagtgacaaa tgccctgctt ccaggaaaag cctctaagca 660 tcaggtaaca tcaaatcgta ccccaaaccg acacaggtgg tcaggtagag aataccaagg 720 cgcttgagag aactcgggtg aaggaactag gcaaaatggt gccgtaactt cgggagaagg 780 cacgctgatg gtaagtgaag tgacttgctc tggagctgaa atcagtcgaa gataccagct 840 ggctgcaact gtttattaaa aacacagcac tgtgcaaaca cgaaagtgga cgtatacggt 900 gtgacgcctg cccggtgccg gaaggttaat tgatggggtt atccgtaagg agaagctctt 960 gagtgaagcc ccggtaaacg gcggccgtaa ctataacggt cctaaggtag cgaaattcct 1020 tgtcgggcaa gttccgacct gcacgaatgg tgtaaccatg gccacgctgt ctccacctga 1080 gacccagtga aatcgaaatc gctgtgaaga tgcagtgtac ccgcggctag acggaaagac 1140 cccgtgaacc tttactgcag cttgacactg aactttgaac ctgtttgtgc aggataggcg 1200 ggagacatag aagcaagagc gccagctcat gtggagtcaa ccttgaaata ccgccctgac 1260 atgtttgaag ttctaactcg atgaagacct caaagaggac agtgtctggt gggaagtttg 1320 actggggcgg tctcctccta aagggtaacg gaggagcacg aaggtctgct gattacggtc 1380 ggacatcgta aggtcagtgc aatggcataa gcaggcttga ctgcgagagc aacaactcga 1440 gcaggtgcga aagcaggtca tagtgatccg gtggttctga atggaaaggc catcgctcaa 1500 cggataaaag gtactctggg gataacaggc tgataccgcc caagagttca tatcgacggc 1560 ggtgtttggc acctcgatgt cggctcatca catcctgggg ctgaagttgg tcccaagggt 1620 atggctgttc gccatttaaa gtggtacgcg agctgggttc aaaacgtcgt gagacagttt 1680 ggtccctatc taccgcgggc gtaggataat tgagagggat tgctcctagt acgagaggac 1740 cggagtgaac gaaccgctgg tttacgggtt gtcatgccaa tggcacggcc cggcagccaa 1800 gttcggaact ggataaccgc tgaatttttt 1830 <210> 10 <211> 3618 <212> DNA <213> Ochrobactrum anthropi <220> <221> rRNA (222) (1) .. (3618) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 10 catcatggga gttggtttta cccgaagcgc tgtgctaacc gcaaggaggc aggcgaccac 60 ggtagggtca gcgactgggg tgaagtcgta acaaggtagc cgtaggggaa cctgcggctg 120 gatcacctcc tttctaagga agatcgagaa taggaaagac gcagtcttcg ggctgatgat 180 ccttctccat cttattagaa catagatcgc aggccagtca gcctgacgat cgctttgcag 240 gcgtgccgcc ttcgtttctc tttcttcatt gttgattgac acttgtaccg ctcacgagcc 300 gtattgcagc tgcgctgctt ggctctgcgc gagcgcgccg catgagcggc gacggactag 360 cgtcctgtat ttggttctaa cggtttgttt gttggttctg atacaagggc ttgtagctca 420 gttggttaga gcacacgctt gataagcgtg gggtccggag gttcaagtcc tcccaggccc 480 accaaattgt gataaggggc catagctcag ctgggagagc acctgctttg caagcagggg 540 gtcgtcggtt cgatcccgtc tggctccacc atcacttttt tggtgtcgag taggacggat 600 agacagtcag tcaacaagag aaagaaccaa gtttgcggac tttacgaagt ctgcgtgttt 660 ctgtatgaaa tcgtgaaaga agatgtaatc ggatcaacta tccagttgat gtcgcaatgg 720 tttgctcaaa ccttgcatta tgattggacg ctaaccgcgc caccgattgt atctcgagaa 780 gctggtcttt ctgctgatat gatcagagct taatgctttg atggatattg gcaatgagag 840 tgatcaagtg tcttaagggc atttggtgga tgccttggca tgcacaggcg atgaaggacg 900 tgatacgctg cgataagcgt cggggaggtg cgaataccct ttgatccgac gatttccgaa 960 tggggcaacc caccttagat agctagaaaa tcaatttagt tggagcaacg ctgttgggtt 1020 taggcccata cagaccgcta ggtcgtcggc ccatgtgggc cgcccccgcg gagcgccagc 1080 atcgtaagat gcgtacggcg cgtgagcgag aactaaatta atttctagtt atcgtaataa 1140 ggtatctaca cctgaataca tagggtgtta gaagcgaacc tggggaactg aaacatctaa 1200 gtacccagag gaaaggacat caaacgagac tccgctagta gtggcgagcg aacgcggacc 1260 aggccagtgg caatagggaa taaagtggaa gaacctggaa aggtttgccg aagtgggtga 1320 tagccccgta cacgtagaac acctgttgtc cttgagtagg gcgggacacg tgaaatcctg 1380 tctgaacatg ggtcgaccac gatccaagcc taagtactcg tgcatgaccg atagcgaacc 1440 agtaccgtga gggaaaggtg aaaagcaccc cgacgagggg agtgaaacag tacctgaaac 1500 cggatgccta caaacagttg gagcccaagg ttcgtcctgg gtgacagcgt accttttgta 1560 taatgggtca gcgacttagt gtatcgagca agcttaagcc ggtaggtgta ggcgcagcga 1620 aagcgagtct gaacagggcg ttcagttcga tgcattagac ccgaaaccaa gtgatctagc 1680 catgagcagg ttgaaggtac ggtaacacgt actggaggac cgaacccata tctgttgcaa 1740 tagatcggga tgacttgtgg ctaggggtga aaggccaatc aaacttggag atagctggtt 1800 ctccgcgaaa tctatttagg tagagcgtcc agcgaatacc cccgggggta gagcactgaa 1860 tgggctatgg ggactcaccg tcttactgat cctaatcaaa cttcgaatac catacagttg 1920 gaagcagagg acagacggtg ggtgctaacg ttcatcgtca agagggaaac aacccggacc 1980 gccagctaag gtccccaagt tctagttaag tgggaaacga tgtgggaagg catagacagc 2040 caggatgttg gcttagaagc agccatcatt taaagaaagc gtaacagctc actggtctaa 2100 ataagggtct ttgcgccgaa aatgtaccgg ggctaaagcc atacaccgaa gctgtggatg 2160 cacatttgtg cgtggtagcg gagcgttccg taagcctgtg aagggacagt cgtgagacat 2220 cctggaggta tcggaagtga gaatgctgac atgagtaacg ataaagggag tgagagactc 2280 cctcgccgaa agtccaaggg ttcctgctta aagttaatct gagcagggtt agccggcccc 2340 taaggcgagg ccgaaaggcg tagtcgatgg gaaccacgtt aatattcgtg ggcctgcagg 2400 tagtgacgga tcgcgtgtgt tgtaaggtct tattggattg atcttgcagc gaagcggttc 2460 caggaaatag ctcctgcata tagaccgtac cctaaaccga cactggtgga ctggtagaga 2520 ataccaaggc gcttgagaga actgcgttga aggaactcgg caaaatgcac gcgtaacttc 2580 ggaagaagcg tgacctccat ttaggcaact aggtgggggt ggcacagacc agggggtagc 2640 gactgtttac caaaaacaca gggctctgcg aagtcgcaag acgacgtata gggtctgacg 2700 cctgcccggt gctggaaggt taagaggaga tgtgcaagca ttgaattgaa gccccagtaa 2760 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga 2820 cctgcacgaa tggcgtaacg acttcccccg ctgtctccaa cgcagactca gtgaaattga 2880 attcccccgt gaagatgcgg ggttcctgcg gttagacgga aagaccccgt gcacctttac 2940 tatagcttta cactggcatt cgtgtcggca tgtgtaggat aggtggtaga ctttgaagca 3000 gtggcgccag ccattgtgga gtcatccttg aaataccacc cttgcctata tggatgtcta 3060 actgcggccc gttatccggg tccaggaccg tgtatggtgg gtagtttgac tggggcggtc 3120 gcctcctaaa gagtaacgga ggcgcgcgat ggtaggctca gaacggtcgg aaatcgttcg 3180 tcgagtgcaa tggcataagc ctgcctgact gcaagactga caagtcgagc agagacgaaa 3240 gtcggtcata gtgatccggt ggtcccgcgt ggaagggcca tcgctcaacg gataaaaggt 3300 acgccgggga taacaggctg atgaccccca agagtccata tcgacggggt tgtttggcac 3360 ctcgatgtcg actcatcgca tcctggggct ggagcaggtc ccaagggtat ggctgttcgc 3420 catttaaagc ggtacgtgag ttgggttcag aacgtcgtga gacagttcgg tccctatctg 3480 ccgtgggtgt aggaatattg ataggatctg atccctagta cgagaggacc gggttggaca 3540 gtctcttctg gtggacctgt ggcctgccac gcagctgggt agcttatacg gacgggataa 3600 ccgctgaaag catcttaa 3618 <210> 11 <211> 2996 <212> DNA <213> Peptostreptococcus prevotii <220> <221> rRNA (222) (1) .. (2996) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 11 ctctcatgga gttggcaata cccgagcctg tgagcgaacc ttttagggcg cagcagtcga 60 aggtagggtc agtaactggg gtgaagtcgt aacaaggtag ccgtatcgga aggtgcggct 120 ggatcacctc ctttctaagg atgagaagct gatacgtcag cttctcacgt gagattttta 180 tctcttagaa ataaaatctc aaagtaactt tggattctgt ataatagttc aaaatagaac 240 caacaaaact aaatagcaaa aaatatttcc agtcaagaaa gaaagggcgc aaggtggatg 300 ccttggcaca tgaaggcgat gaaggacgta agtgaacgaa aactagggag agctcacaaa 360 aagcactgac ccctaggtct ccgaatgggg aaacccggct gtggaagaca cagtcattac 420 taagtgaata catagcttag taaagcaaga ccctgtgaac tgaaacatct aagtaacagg 480 aggaaaagaa agaaaactcg attttccaag tagcggcgag cgaaaagaaa agagcctaat 540 ccattgagga atatctagtt agtcgaatca tctgggaaga tgaaccaaag aaagtgaaag 600 tcttgtagac gaaaactaaa taaaccaggg aggaaagtag caccggacac gaggaatccg 660 ttgtgaagat agggggacca tcccctaagg ctaaatacta acatgtgacc gatagcgaac 720 aagtaccgtg agggaaaggt gaaaagaacc cccgaaaggg ggagtgaaac agaacctgaa 780 acctagtgcc tacaagcaga gagagctcta aagagtgatc tcgtaccttt tgtagaatgg 840 gccagcgagt tatcgtatat agcaaggtta aatcttttaa gagatgaagc caaagcgaaa 900 gcgagtctta ataggggcga atagttagat gcgatagacc cgaaaccggg tgatctatcc 960 atggtcagag tgaaggtgaa gtaaaattca ctggaggctc gaaccgggtc ggtttaaaac 1020 gtatcggatg aactgtggat aggggcaaaa accaaacgaa ctcggatata gctggttctc 1080 ctcgaaatag ctttagggct agcctttgat taagatttaa ggaggtagag cactgaatgg 1140 tctagggcgg cttaccgtac caaaacctat caaactccga atgccaaaaa atcaatcaag 1200 gagtcagact tagggggata agctttaagt cgaaagggaa acagcccaac cgacagctta 1260 aggtcccaaa attggattaa gtggaaaacg atgtgggaag gcatagacag cttaggaggt 1320 tggcttagaa gcagccaccc tttaaagaaa gcgtaatagc tcactagtcg agtcggcctg 1380 cgcggaagat gtaacggggc taaaactatg tgccgaagct gcggatttga cattagtcaa 1440 gtggtagggg agcgttctgt aagccgatga aggtgtattg agaagtatgc tggaggtatc 1500 agaagtgcga atgctgacgt gtaagtaacg ataaaggggg tgagaaaccc cctcgccgca 1560 agactaaggt ttcctgatca acgctaatcg gatcagggtt agtcgggtcc taaggctcag 1620 ccgaacggtg aggccgatgg cagaacaggt taatattcct gtactaccta taagagtgat 1680 gtggagacgg aggagtgaca acgccgcgga ctgacggaat agtccgttaa agggtgtaga 1740 tgttgattat cccaggcaaa tccgggataa gagtcgaacc tgaagtatac caatttcctc 1800 ggaaaacggt aatagtgcgt gtaaacatac tcccaagaaa atccgctaaa cttaatctta 1860 taggtacccg taccgtaaac ggacacacgt agtcgggttg aatatactca ggcgcttgag 1920 tgaatcactt tgttaaggaa ctcggcaaaa tgtccccgta acttcgggag aaggggagcc 1980 agagcgatct ggccacagaa accaggccca agcgactgtt taccaaaaac acaagtttct 2040 gcaaaatcga aagatgaagt ataggagctg acacctgccc ggtgctggaa ggttaagggg 2100 aaggcttagc ataagcgaag gctagaactt aagccccagt aaacggcggc cgtaactata 2160 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggtgtaa 2220 cgatttgggc actgtctcaa caaaggatcc cggtgaaatt gtagtagtcg taaagatgcg 2280 aacttacccc acgctaggac ggaaagaccc cgtggagctt tactgtaggc tgatattgga 2340 ctttgagatt agacgtacag gatagttggg agactttgaa acacgcacgc cagtgtatgt 2400 ggagtcaccc ttgggatacc aaccctctaa tattaaagtt ctaacgatga cccttgaatc 2460 agggcatcgg acattgtcag ttgggcagtt tgactggggc ggtcgcctcc caaaaagtaa 2520 cggaggcgtt caaaggttcg ctcagaatgg acggaaacca ttcgtagagt acaaaggcag 2580 aagcgagctt aactgcaaaa cctacaagtt gcgcagagta gaaatacgga cttagtgatc 2640 cggtggcacc gcatggaagg gccatcgctc aacggataaa agctaccccg gggataacag 2700 gcttatctcc cccaagagtc cacatcgacg gggaggtttg gcacctcgat gtcggctcgt 2760 ctcatcctgg ggctgaagta ggtcccaagg gttgggctgt tcgcccatta aagaggcacg 2820 cgagctgggt tcagaacgtc gtgagacagt tcggtcccta tccagcgtgg gcgtaagaaa 2880 tttgagagga tctgtcccta gtacgagagg accgggatgg acacacctct ggtgtaccag 2940 ttgttccgcc aggagcatag ctgggtagct acgtgtggaa ttgataagcg ctgaac 2996 <210> 12 <211> 2047 <212> DNA <213> Porphyromonas gingivalis <220> <221> rRNA (222) (1) .. (2047) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 12 atttatgaaa gttggtaaca cccgaaccgg tggcctaacc gttgtggggg agccgtcgaa 60 ggtgggactg gtgattagga ctaagtcgta acaaggtagc cgtaccggaa ggtgcggctg 120 gatcacctcc tttctaagga gtttttgtga gtggaatgtt ggcgtcctgc ctgtgatggg 180 tggggttgag ggcatgctgt tgggttgtgg ggtatcacat gtgtggtggc ctgtgcggtg 240 ctgtgttgcg tgcgtgtgtg cctggtgtgg tgttcgtgtg gtggttgaga actgtatagt 300 ggatgcgagt atctttattg ttgtatcgtt tgtgcgacgt ggtgttgtgc aaagttgtgt 360 agtgcgatcg gtggatgcct tggcaccaag agccgatgaa ggacgttgtg acctgcgata 420 agccctgggg agttggtgag cgagctgtga tccgggggtg tccgaatggg gaaacctgga 480 atgtccggag tagtgtccgg tggccctgcc ctgaatgtat aggggtgtgg gtggtaacgc 540 ggggaagtga aacatcttag tacccgtagg aagagaaaac aagtgtgatt ccgtgagtag 600 tggcgagcga aagcggagga ggctaaaccg tgtgtgtgtt caagccggca ggtgttgcat 660 gtgcggggtt gtgggggcct tgtggttctg ctgccgcagg attggccagt gagaaatgtt 720 gcgtgaaggt gaagcgtctg ggaaggcgta ccggagtggg tgagagtcct gtaactgtaa 780 gcgtggcact ggtgtggggt tgccccgagt agcgtgggac tcgtggaatt tcgtgtgaat 840 tggcggggcc catcccgtaa ggctaaatac tcccgagaga ccgatagtga accagtaccg 900 tgagggaagg tgaaaaaacc tcgaacagag gactgcaatg accctgaacc cgtctgccta 960 caagcggtag gagcgccatt aaggtgtgac ttgcgtgcct tttgcataat gaacctacga 1020 gttactgttt gtggcaaggt taattgttat aatcaagaca aggagccgaa gcgaaagcga 1080 gtcttaaaag ggcgcccatt tagtcacgag cagtagacgc gaaaccaagt gatctaccct 1140 tggtcaggtt gaaggttagt aacactaact ggaggaccga atcggtagcg ttgaaaagct 1200 ttcgaatgaa ctgagggtag gggtgaaaag gctaatcaaa cttgggagat agctcgtact 1260 ccccgaaatg catttaggta gcgcctcgga cgaataccat agggggtaga gcactgtttc 1320 ggctaggggg tcatcccgac ttaccaaacc gatgcaaact ccgaatacct atgagtacta 1380 tccgggagac agactgcggg tgctaacgtc cgtagtcaag aggaaaacaa tccagaccgc 1440 cagctaaggc cccaaaatca tagttaagtg ggaaacgatg tgggaaggca tagacagctt 1500 aggaggttgg cttagaagca gccacccttt aaagaaagcg taatagctca ctagtcgagt 1560 cggcctgcgc ggaagatgta acggggctaa aactatgtgc cgaagctgcg gatttgacat 1620 tagtcaagtg gtaggggagc gttctgtaag ccgatgaagg tgtattgaga agtatgctgg 1680 aggtatcaga agtgcgaatg ctgacgtgag taacgacaaa acgggtgaaa aacccgttcg 1740 ccgaaagacc aagggttcca gggtcaagtt aatctgccct gggttagtcg gggcctaagg 1800 cgaggccgac aggcgtagtc gatggagaac gggttgatat tcccgtacca gtgaaggacc 1860 gtccatactg atattgtgat gctaaccatg ccgatcacgg tgtcatgaag tttttctttg 1920 tggtgttgtg gggtgtgtgg gacccaagct ttggaggtaa gcgtgttaac aggtgtgacg 1980 cagaaggtag ccaagccacg cggtggttgt cgtggtctaa gcgtgtagga tgactggttg 2040 ttaaatg 2047 <210> 13 <211> 3567 <212> DNA <213> Peptostreptococcus anaerobius <400> 13 agcttgacat ccctcggacc ggtgtttaat cacaccttcc cttcggggct gaggtgacag 60 gtggtgcatg gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc 120 gcaacccttg tctttagttg ccagcattca gttgggcact ctagagagac tgccagggat 180 aacctggagg aaggtgggga tgacgtcaaa tcatcatgcc ccttatgctt agggctacac 240 acgtgctaca atgggtggta cagagggttg ccaaaccgtg aggtggagct aatcccttaa 300 agccattctc agttcggatt gtaggctgaa actcgcctac atgaagctgg agttactagt 360 aatcgcagat cagaatgctg cggtgaatgc gttcccgggt cttgtacaca ccgcccgtca 420 caccatggga gtcggaaaca cccgaagccg attatccaac cgcaaggagg aagtcgtcga 480 aggtggcgtc gataactggg gtgaagtcgt aacaaggtag ccgtatcgga aggtgcggct 540 ggatcacctc ctttctaagg agaattacct gctgttcgat tttgaaagtt catactttca 600 aaatttgtac cttgaaaact gaataattta gtgattacaa aagacatcta tcaagttaaa 660 cttgataaaa tatatgagag aaaactcatt aaaaaacacc aattattctt ttaacactgg 720 tcgaaagacc aaaattagaa aaatctcatt aataactggt caagttatta agggtgcagg 780 gcggatgcct tggcactagg agccgatgaa agacgtgata agctgcgata agcttgacat 840 ccctcggacc ggtgtttaat cacaccttcc cttcggggct gaggtgacag gtggtgcatg 900 gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc gcaacccttg 960 tctttagttg ccagcattca gttgggcact ctagagagac tgccagggat aacctggagg 1020 aaggtgggga tgacgtcaaa tcatcatgcc ccttatgctt agggctacac acgtgctaca 1080 atgggtggta cagagggttg ccaaaccgtg aggtggagct aatcccttaa agccattctc 1140 agttcggatt gtaggctgaa actcgcctac atgaagctgg agttactagt aatcgcagat 1200 cagaatgctg cggtgaatgc gttcccgggt cttgtacaca ccgcccgtca caccatggga 1260 gtcggaaaca cccgaagcct gattatccaa cctkysagga ggmagtcagt cgaaggtagg 1320 cgtcagataa ctggggtgaa gtcgtaacaa ggtagccggy atcggaaggt gcggctggat 1380 cacctccttt ctaaggagtc aaaktacgwt rctgttcgat ttttgccaaa agssmattac 1440 ttasmaaaac tttgtacctt garaactraa gtaaacttta gtagaytaca aargttacat 1500 ggwggtgtag ctcaagttgg gagagcacyt gmcttgmamg cagggggtca tatgagstcg 1560 aaasctcatt masasmcacc aagttattct tttamcannc tggttnttcg atsagaacca 1620 saattaraat taaatagctc atsaatatts cyrgtcaags saksaagggy rcarggtgga 1680 tgccttggca ctakgaagwc gatgaaagga cgtgataagc tgaacgataa actagggtga 1740 gctcacaaaa agcactgacc cctaggtctc cgaatggggc aacccggttg tggaagacac 1800 aatcattact aagtgaatac atagtcttag taaggcgata ccctgcgaac tgaaacatct 1860 aagtagcagg aggaagagaa agaaacatcg attttctaag tagcggcgag cgaaaggaaa 1920 agagcctaac tcattagcga tatcttaaag ttagtcgaat tatttgagaa gataaaccaa 1980 agaaagtgac agtcttgtag acgaaagctt taagatacga gagggaaagt atcaccgggc 2040 acgagtaacc cggtgtgaag atagggggcc catcccctaa ggctaaatac taacatggac 2100 cgnatagnna acaagccatn tgatggatga tggtctaaag ccgtaggaag aaaggtaggc 2160 aaatccgcct ttcacaaatt ctgaaagctg ataggtatcg aaaacataag tagagaagtt 2220 gatgactcca tgttgccaag aaaagtcact atccagacca aagcgcccgt accaaaaccg 2280 acacaggtag ggaggtagag aataccaagg acgcgcggna agaaatcngg ctttgttaag 2340 gaactcggca aaatgtcccc gtaacttcgg gagaagggga gcctgagaga tcaggccaca 2400 gaaaccaggc ccaagcgact gtttaccaaa aacacaagtt tctgcaaaat cgcaagatga 2460 agtataggag ctgacacctg cccggtgctg gaaggttaag gggaaggctt agagcaatcg 2520 aaggctagaa cttaagcccc agtaaacggc ggccgtaact ataacggtcc taagatatat 2580 atntatatan atatntntnt atanatatat atatatatat ctatatagat atatatctat 2640 atatatatat atatatatat atatatatat atatatatat atatatatat atatatatat 2700 atatatatat atatatatat atatatatat atatatatat atatatatat atatatatat 2760 atatatatat atatatatat atatatatat atatatatat atatatatat atatatatat 2820 atatatatat atgatagtag tagtagtngt agtaatagta gtagtagtag tagtagtagt 2880 agtagtagta gtatgantag tagtagtgat tactagtagt agtgagtagt gatcagtagt 2940 gatcagtagt actattagtt agtnagtgat gagtagtgat gagtaattat gagtagtagt 3000 natgatgagt gagtagtcag tgatnagtna tcagtaatca tgagtgatna gtnagtagta 3060 gtgagtagtg agtagtgagt aatnagtagt gagtagtgat nagtagtgag tgagtgagtg 3120 atnagtnatn agtgagtnat gagtgagtna tgagtagtna gtgagtgagt nagtnatgga 3180 tnagtnagtn agtgagtagt gaatnagtta atnagtngtc agtgagtgag tnantcagtc 3240 atnantnagt catgagtgag tgagtgatga gtgactgact catcagtgat gatcgtgang 3300 atgatcatca tannatggtg agtatgatat atgagtacta ctatagtata tgatatatat 3360 accgtatata tatngtatat cgtatnggnt ggtatagtat ngacgctata tcgtgtacgg 3420 accnataccg tgnntggtgc ggtagtgata tatntntgna nactgtaccn tagatngata 3480 gntccgggta gatcgctcca taggtgtactac tgtagatggc ctctagtctg gntcggtagn 3540 cggggttcgg gccccanaan ngtgncn 3567 <210> 14 <211> 3104 <212> DNA <213> Peptostreptococcus magnus <400> 14 cccccgccnc cncnagagtt gataacnccc naagccggtg gcntgnccgc aagnaaggaa 60 gctgtntaag gtgggatnna tgantngggn ngangtggtn cnaaggattc ccnnccccgg 120 naanggggga nggatcacct cctttctang taagaaacgc atgatcgaag atgatgcaca 180 agagaagtga tgtttcgaag atactgttta gttttgagtg atactcaggt atgactcaga 240 gatcattgan aactggataa tagaaaataa attgcgatag aaatagaaaa tgagatcgaa 300 atagtaattg ttattattga ggtcnttcta gaaaagtcga aaactaagaa ttttcatcac 360 taaaaaacat aaatcgaaac ggatggatgg attttagtaa atagttgaaa atagtaatca 420 agcaaacacc aaaataggta aagtaagaaa gagcgtatgg cggatgccta ggcacagaga 480 ggcgaagaag gacgcagcaa acagcgaaat gcgacggtta gcagtaagca tgcaacgatc 540 cgtcgatgtc cgaatggggg aacccacctg gagtagagac caggtatcct gaagtgaata 600 aatagcttca gagaggcgag actcagggaa ctgaaacatc taagtacctg aaggcaagag 660 gaaaataaac gaatgattcc ctaagtagcg gcgagcgaaa ggggaagagc ccaaaccgat 720 cttaggatcg gggttgtagg actgtcggca aagagcaaga aatcattata ggcgaacgga 780 atgggaagtc cggcgaaaca gggtgacagc cccgtagccg aaatagtgaa gaagcacgag 840 acagcacctg agtacggcgg gacacgagng aatcnngctg tacgraaatm canccaggna 900 ccatactggt aaggactaaa tactacctac tgtgnaccgt ataggtgaac acagtaccgt 960 gagggaaagg tgaaaagaac cccgngagng gagtganata gaacctgana ccatatgctt 1020 acaagaagtt acgagcccgt taaagggtga tagcgtgcnt tttgtataat gaaccggcga 1080 gttacnatat ggagcgaggt taagcaggat ntgcggagcc gaancganag cgantcttaa 1140 cagggcgaaa gttgcatgtn ntataccnna aaccnagtga tctatccatg accaggttga 1200 anttggggta aaacccgatg gaggaccnaa ccgncccccg ttgaaacgtt ggcggatgag 1260 ttgtggctag gggtgaaatt ncaatcgaac tcggagatag ctggttctcn ccnaaatagc 1320 ttnagggcta gcgtcgaggt aaagtcgtgt gaaggtacag cacgtgaata tgtgatggcc 1380 ccatctcggg gtacttgaac ataatcaaac tccgaatgtc acaaanatat ncttancant 1440 cngacagcgn gtgatnaagt tcattgtcna anngtaaaca ccnccatacc atcnactnnc 1500 ngtccccaan nntatatact aagtnnaagn agatgtggga gantttcnaa cnaacntncg 1560 nnngtttccc ttcccantcc ntcccatttt ttnnancagt gcnntanccn ccctcatttn 1620 ggtcgnnntg gactctngct ccnantattn ncccgngncc tnagnnnact tacccggaan 1680 tngntggaan ttncccctta annggagnng annanttnnn gggngntnng tnntatnnnn 1740 aaanttatgn attttangng tnagcgtgag tggtagggga gcgttntatg tgcggagaag 1800 gnggtacngt aaggagcngt ggagcgcata gaagagagaa tgccggtgtg agtagcgaaa 1860 cgtgggtgag aatcccacgc accgaaaacc caaggtttcc agaggaaggt tcgtccgctc 1920 tgggtaagtc gggacctaag scgaggccga gaggcgtagt cgatggacaa caggtagaga 1980 ttcctgtact tacggtatga atgatggagt gacggagaag gctagcggat cctgctgatg 2040 gaaatgcagg tgcaagcgag gtagccgaca gccaggcaaa tccggctgtc gaaaggcaaa 2100 ggcgtgaggc gtatggaaag ctgcggcaag tacagaagtc cgtgaagcca gcttccaaga 2160 aaagcttcta gtgataatca tacagtaacc cgtaccgaaa atggacacac atgggtgagg 2220 agagaatcmt aaggtgagcg tagagcaact atagctaagg aactctgcaa aatgactccg 2280 taacttaggg ataaggagtg ctcatagaga tatgagccgc agtgaaacgg cccaagcgac 2340 tgtttaccaa aaacacagct ctatgctaag tcgaaagacg acgtatatgg ggtgacgcct 2400 gcccggtgct ggaaggttaa gaggatgtgt cagcgtaagc gaagcattaa ttaagcccca 2460 gtaaacggcg gccgtaacta taacggtcta agtnananac atgcacncca gtgtgtnagg 2520 agtcaatgtt ggnanannac tnttctngta ttgnagttnt anccgnatgc catgnaantg 2580 gcaacgggac agtgtcaggt gggcagtttg antggggcgg tcgcctccca aagagtaacg 2640 gaggcgccca aagataccct cagcttggat ggaaatcaag cgcagagtgc aaaggcataa 2700 gggtgtttga ctgcgagacc tacaagtcga gcagggacga aagtcgggct tagtgatccg 2760 gcggtgctga atggaaaggc cgtcgctcaa cggataaaag ctaccctggg gataacaggc 2820 tgatctcccc caagagttca catcgacggg gaggtttggc acctcgatgt cggctcatcg 2880 catcctggag ctgaagtcgg ttccaagggt tgggctgttc gcccattaaa gcggtacgcg 2940 agctgggttc agaacgtcgt gagacagttc ggtccctatc tgtcgtgggc gtaggaagtt 3000 tgagaagatc tgtcctcagt acgagaggac cgggatggac atatcaatgg tgcaccagtt 3060 gtcacgccag ggcacagctg gtagnctaaa tangaaggga aaan 3104 <210> 15 <211> 4243 <212> DNA <213> Fusobacterium necrophorum <400> 15 cccnccgtca acaccgacgc anagttggtt gcaccctgna agkagcaggc ctaaccttag 60 ggaaggatgc tccgagggtg tggttagcga ttggggtgaa gtcgtaacaa ggtatccgnt 120 acgggaacgt gcggatggat cacctccttt ctaaggagta ttcttgttgt tctttcttct 180 ttggaagggt tcgcgcatgg accttggaaa ctgtatagta gatcaaaaaa caaacaagaa 240 taaagaacaa agaactctag tttctagagt tagctgscaa agaaaamaac msgdttncaa 300 agtamcaaag ggcacacaag ggatgcctag gtagaaagag ccgaawaagg acgtggtaag 360 ctgcgataag cttggcgaag ttgcaaacga acgtggatgc caagatytcc gaatggagca 420 atctgtaaag agtcatgtct ttacacgaaa gagggaaccg ggtgaactga aacatnctaa 480 gtaatnccgn aggaaaagta aagtaacaac gataccctaa gtagcggcga gcgaacgggg 540 tagagcctaa accgtattca tgtcaaggat gcagccgttg tggatatggg gtagcngggn 600 aanagagara ranaagaact gcaagnctat ttcgcagaac gtaagcaaag gaacaagaaa 660 gatctgggaa aggtctaccg tagaaggtga aagtcctgta ttggtagntt ttnnnncttn 720 nnnnaaanng cgctgntatm tcttctnnnn nncccnnaag ggnngntaat gnnnnnnntt 780 ggnaacacnn gnargaannn nnnnnnnccn nnnttctnnn ngcasraatn ccccntnggw 840 gnantnnnnn nggacnncaa atnctcgtaa ggctanaata ctctctgcga ggtacccaam 900 tagctacgta mggtctmrat agwyggnncs tnasckaacn cgatagtcgc aymgtrccgt 960 gtagggaaag gsgaaaagaa ccccggwagg ggagtgaaag agaacctgaa attgtgtgct 1020 tacaagcggt cagagccact tyggtggtga tggcgtgcct tttggagaat gatcctgcga 1080 gttacgtttc atggcgaggt taagaagaac ggagccgaag ggaaaccgag tctgaatagg 1140 gcgcaagagt cgtggagcgt agacgcgaaa cccggtgatc taagcctgtc caggatgaag 1200 ctgtggtaag acacagtgaa gtagcaggcc taaccttagg gaaggatgct ccgagggtgt 1260 ggttagcgat tggggtgaag tcgtaacaag gtatccgtac gggaacgtgc ggatggatca 1320 cctcctttct aaggagtatt cttgttgttc tttcttcttt ggaagggttc gcgcatggac 1380 cttggaaact gtatagtaga tcaaaaaaca aacaagaata aagaacaaag aactctagtt 1440 tctagagtta gctgacaaag aaacancaag gttaaagtac caaagggcac acaagggatg 1500 cctaggtaga aagagccaac aaggacgtgg tagctgcgat aagcttgcga agttgcaaac 1560 gaacgtggat gccaagatct ccgaatggag caatctgtaa agagtcatgt ctttacacga 1620 aagagggaac cgggtgaact gaaacatcta agtaatccga ggaaaagaaa gtaacaacga 1680 taccctaagt agcggcgagc gaacggggta gagcctaaac cgtattcatg tcaaggatgc 1740 agccgttgtg gatatggggt agcgggaaag agaaagaaag aactgcaagc tatttcgcag 1800 acgtaagcaa aggaacaaga aagatctgga aaggtctacc gtagaaggtg aaagtcctgt 1860 attggtagtt ttcttgcgct gtatctcttc tcccaagtaa tgtggaacac gaggaattct 1920 gcatgaatct gcgaggacca aatctcgtaa ggctaaatac tctggaggtn cctaacccac 1980 cgccgtatga aaagttgggg gatgaggtag gtttankggg tgaaaagcca stckaaccng 2040 ggagatagcc tcgttctctc cgaaatgcat ytacwgtgcm gtccttgcgt tgtnttaatg 2100 atgggggtag mawcactgac tgatactakw ggggcstata tgcttactga anttcaatgc 2160 aaactccnga ataccsttta tntcaagakc gcagtgagtn gagacccatg gtgnagttma 2220 cttnccatcg tnnccgasat ggtcgaaacc amtttttccc agaccaccag mnnnttnaag 2280 ggatcccssa ttcsgtatct tamgtggtgc aaaagggagg ttgtgcakat tnctntaaac 2340 aantnnaggc agnaaaanng tgttngnnng cntgnattnt ntantgntnt nanttngttt 2400 nnnnacntnn nngtttnttt ttnntntttn tccnntantc ntnntctccc tcnncntnnt 2460 nnntnagttn ntttattatt tgatgncnng attctnnttt gtntntttct nttngnnntt 2520 nnttccnncc nnccccttcg ttnnatcgnc gtaaggtccc nnatcatatn taagntnnnt 2580 tgnggaaagn aggtggagmt tcttaanaca acntaggagg ttggcttaga agcagccatt 2640 ccttgaaaga gtgcgtaaat agctcactag tcgagagtct ctgcgccgac aatgtaacgg 2700 gngctaagat atgaaccgaa gctgtggatg tcgtaagaca tggtaggaga gcgttctgta 2760 ggccgtcgaa ggaggactga aaggaactct ggaggtatca gaagtgagaa tgcaggaata 2820 agtagcgasa aggggagtga gaatctcccc cgctggaaga ccaaggtttt cagggtaaag 2880 cttgtcttcc ctgagtaagc cgggacctaa gcccaggcta gaatgcgtag gcgtaatgga 2940 aaacagnats aatatttact gtgccagttc ctagctttgt gaaggtaggg aacgccagaa 3000 gggtatgcgc gcagacgaac ggaagagtct gtagaagcat gtagagtgac ttggtaggca 3060 aatccgccag gttagacttg aggtgtgaca tatactcgca agaggaatgc gcaaatccca 3120 cgctgccgag aaaagcttct agcggtaaag tagagactgc ccgtacttgg ataaccgacc 3180 cacaggtggt caggatgaga aatayttaaa gcggtmcmgg gctracwywc gttaaaggaa 3240 ctctgccaaa atggccccgt aacttccggg aagaaggggt gcctcttggt gtgagtatac 3300 aagcnataca aagcgcanag aggtcgcagt gaagaggctc aagcaactgt ttawcaaaaa 3360 cacaggtcta tgcgaagctg taaggcgaag tatatgggct gacacctgcc cagtgccgga 3420 aggttaanka ggaggtagtg agartctacc gcaattggaa ggccccggtg aacggcggca 3480 cgtaactata acggtcstaa ggtagcgaaa ttccttgtcg ggtaagttcc gacctgcacg 3540 aatggtgtaa tgatttgagc gctgtcttga cgggaggcct ggtgmmattg tattaccggt 3600 gaagataccg gttacctaca gtaggacgga aagaccccat gsagctttac tgtagcttgg 3660 tattgggttt tggcatggca tgtataggat agttgggaga ctgggaaggt atggcgctag 3720 ctgtaccgga gtcatcggtg gaataccaac cattccctgc tgaaattcta atctgtactt 3780 tggaggtatg gagacagtgc taggtgggca gtttgactgg ggcggtcgcc tmacraaaga 3840 gtaacggagg crtwcaaagg ttctactcag gttggatgga aatcaaccgc agagtgcaag 3900 ggcaaaagag agtctkgact gcaagaactg acgggtcgag cmgatgcsaa agcatgacat 3960 ngngatccgg ccatttcgna tggaagggtc ntnnctcaaa aanaaaaann nctnccctgg 4020 agatnacagg ctgatcctac ccgaaaattc atatggacgg gnnggtttcn cancttgatg 4080 tcngctcatc cnatgntggg gnaagaaaaa annncccaag ggntgggctn nnccnnnnaa 4140 naaannngna ncntnanctn ggaannaaaa cnnnaaaaaa aannnnccgn tccnatnnag 4200 ngggnngnnn nannaatnnn nnnnannnnc tntncnnaaa nan 4243 <210> 16 <211> 2625 <212> DNA <213> Proteus vulgaris <400> 16 nncatgggta gtgggttgca aaataagtag gtagcttaac cttcgggagg gcgcttacca 60 ctttgtgatt catgactggg gtgaagtcgt aacaaggkaa cccgtanggg gaacctgcng 120 gttggatcmc cycccttmcc taaragnata cgtgttatgt gcmgkgnnnc tcacacagac 180 ttgtytkatk aagaacgagc aaaangsgsg tytgcgaaag ctgacntgaa gtcccccttc 240 gtytagaggc ctaggmcmcc sccctttcac ggcggtaaca aggggktcga atccccntag 300 gggacgccaa ttgcgcggta tgagtgaaag gsgtcccmcm ctatagtytg atgcmaatca 360 aanaawagtt aagataattt tagcaagtta ttttaactat tatgctyttt taacmatctg 420 gaaccagctg naaaattgaa aaccaatcca tntatccccn aggnataatn atgagtntnt 480 ccaaaatntc aaactttgna tngttttttg ccntcgaagt gggangancg agccatttac 540 ngtttgaggc ggccngngnn cngtnagngc cacnnacntt annangntag nnnggcganc 600 cctgccccac nacnnaangn natttgnncn nccntccccn cccnnacnng tcattaaaaa 660 gaaacatctt cgggttgtga ggttaagcga ataagcgtac acggtggatg cctaggcaat 720 cagaggcgat gaaggacgtg ctaatctgcg ataagcgtcg gtaaggtgat atgaaccgtt 780 atacccgacg atttccgaat ggggaaaccc aatatccaat ggatattatc attaactgaa 840 tacataggtt aatgaagcga accgggagaa ctgaaacatc tcagtacccc gaggaaaaga 900 aatcaaccga gattccccta gtagcggcga gcgaacgggg aacagcccag agtcttaatc 960 aacagcagca tcaggagaac ggtctggaaa gtccggcagt aaagggtgat agccccgtat 1020 ccgaagatgc tgttattgtg aactcgacga gtagggcggg acacgtgttc cmtccttgwc 1080 tagaatatgg ggggaccatc ctgccaaggc taaatactnc ctgawtgacc gnatagtgaa 1140 cccaangtac cgtgagggaa aggcgaaaag aaccccggcg aggggagtga aaaagaacct 1200 gaaaccgtgt acgtacaagc agtaggagcc ccancactaa gctnntggtg aactcacann 1260 nattcttttg catgataaan agcggnagac gacgcnaagt gacgtccaac caatccaatc 1320 aggcagagga ggcttagtgg tggggtgact gcgtaccttt tgtataatgg gtcancgact 1380 tatattctgt agcaaggtta accgaatagg ggagccgtag ggaaaccgag tcttaactgg 1440 gcgaatgagt tgcanggtat acacccgaaa cccggtgatc tatccatggg caggttgaag 1500 gttgggtaac actaactgga ggaccgaacc gactaatgtt gaaaaattac cggatgactt 1560 gtggatgggg gtgaaaggcc aatcaanccg ggagatagct ggttctnccc gaaagctatt 1620 taggtacngc ctcgtgaact catcttcggg ggtagagcac tgtttcgact aggggggtca 1680 tcccgactta ccaactcgat gcaaactgct aataccgaag aatgttatca cngnagacac 1740 acggcgggtg ctaacgttcg tcgtgaagan ggaaacaacc canaccgcca gcttaggtnc 1800 caaagtcatg gttaagtggg aaacnaagtg ggaaggctca gacagccagg atgttggctt 1860 acaaccaccc ttatttnaag gaaagtcnct gngaagatgn agtgnagccn cngcnngacg 1920 gagagacccc gcgaacctnc cntatagctt gacactgaac atngagcctc cactgtgtag 1980 gnataggtgg gagactatga agtgtggacg ccagtctgca tggagtcanc cttgccaata 2040 nnccaccctt taacgtttgg atgttactaa cctaggcccn gtaatcncgg gtncgggtwa 2100 mcgtgtntcg cccgtgggta gtttgactgg ggcggtctcc tcctaaagag taacggagga 2160 gcacgaaggt tggctaagca tggtcggaca tcatgcggtt agtgcaaagg cataagccag 2220 cttgactgtg agagtgacgg ctcgagcagg tacgaaagta ggtcttagtg atccggtggt 2280 tctgaatgga agggccatcg ctcaacggat aaaaggtact ccggggataa caggctgata 2340 ccgcccaaga gttcatatcg acggcggtgt ttggcacctc gatgtcggct catcacatcc 2400 tggggctgaa gtaggtccca agggtatggc tgttcgccat ttaaagtggt acgcgagctg 2460 ggtttagaac gtcgtgagac agttcggtcc ctatctgccg tgggcgttgg aagattgaga 2520 ggggttgctc ctagtacgag aggaccggag tgaacgcacc actggtgttc ggggttgtca 2580 tgccaatggc attgcccgag tagctaagtg cggnaagcag ataac 2625 <210> 17 <211> 4372 <212> DNA <213> Enterobacter aerogenes <400> 17 cccggnaagg ggccccaann ncnngggtna antngnnann nnantnngga agggnnnacn 60 nnanccnagg ggggnnggnc ntnntnnncc ncccnccntt ttaanaaaag gnngaaacnc 120 cnttnncnnn annggngcct gcggnannat nncancgggn cnttananna anccgccnnt 180 cacgccatcn accatgwgta gtgggttgct nangaaanaa ngtaggtaag cttaacmttc 240 twwrcagwgc ngctgtacca ctttcwygat tcatgacttg wwwywanang tcgtracaag 300 gtamccgtag grgaacctgc ggkyggnatc acctcctymc cnttaanaga rccsgcckty 360 gcagygctca cancagattg tctgatgraa gtaanagaag caaggcgtct tgcgattgag 420 mcttcagtgt ccccttcgtc tawaggccca rgacaccagn ccnstntcac wwcggtaacc 480 aggggttcna grtcccctak kgganncgcg crcatbgctc wtcgttmgtg awtgamagac 540 gcttgaccng aacatattct caagaaytcm tcttcgggtg nrcgttggnn agakatttgc 600 tctttaaaaa tctggatcaa gctgamaatt gaaacgacac acagtctsat gtgtgttacg 660 agtcttctca aatttttcgc gtacacggat gaatgtttts ynaygaaaca tcttncgggg 720 ttgtdgaggt taagcgnact amgacgkacm cggtgngatg ccctggcawt cagakgcrat 780 gaakgacgtg ctaanatact gcgaaaagcg tcggtaaggt gatstgaacc agnttacaac 840 cggcgatgtc cccggnaagg ggccccaann ncnngggtna antngnnann nnantnngga 900 agggnnnacn nnanccnagg ggggnnggnc ntnntnnncc ncccnccntt ttaanaaaag 960 gnngaaacnc cnttnncnnn annggngcct gcggnannat nncancgggn cnttananna 1020 anccgccnnt cacgccatcn accatgwgta gtgggttgct nangaaanaa ngtaggtaag 1080 cttaacmttc twwrcagwgc ngctgtacca ctttcwygat tcatgacttg wwwywanang 1140 tcgtracaag gtamccgtag grgaacctgc ggkyggnatc acctcctymc cnttaanaga 1200 rccsgcckty gcagygctca cancagattg tctgatgraa gtaanagaag caaggcgtct 1260 tgcgattgag mcttcagtgt ccccttcgtc tawaggccca rgacaccagn ccnstntcac 1320 wwcggtaacc aggggttcna grtcccctak kgganncgcg crcatbgctc wtcgttmgtg 1380 awtgamagac gcttgaccng aacatattct caagaaytcm tcttcgggtg nrcgttggnn 1440 agakatttgc tctttaaaaa tctggatcaa gctgamaatt gaaacgacac acagtctsat 1500 gtgtgttacg agtcttctca aatttttcgc gtacacggat gaatgtttts ynaygaaaca 1560 tcttncgggg ttgtdgaggt taagcgnact amgacgkacm cggtgngatg ccctggcawt 1620 cagakgcrat gaakgacgtg ctaanatact gcgaaaagcg tcggtaaggt gatstgaacc 1680 agnttacaac cggcgatgtc cgaatwggga aacccagtgc aaattcgtyg cactatygtt 1740 aactgaatac atargttaac waggcgaacc cgggggaact gaaacattct aaktaccccg 1800 aggaaaaraa akcaaatcaa ccwmwattnc ccccagtagc garmrarcga acggggagca 1860 gcnnccnann nnnntgagtn nnnactgaga tcannnnnna gccaanagtt taaataagtt 1920 tngtgtttag tggaacggtc tggaaarttc cgacggtaca gggtratagt ccsgtacmcc 1980 caaaatscmc aggttgtgta actygaaraa gtagggcggg aacacgtggt antcctgtyt 2040 raatatgggg ggaccatcct ccaaggstta aataytcctg actgaccgta tagtgamcca 2100 gtaccngtga ggraaaggcg aaaagaaccc cgggcgaggg gagtgaaaaa gaaccttgaa 2160 accngtgtac gtacaagcag tgggagcacc tttcggggtg tgactgcgta ccttttgtat 2220 aatgggtcag cgacttatat tctgtagcaa ggttaaccgt ataggggagc cgcagggaaa 2280 ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca 2340 tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa 2400 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct 2460 ccccgaaagc tatttaggta gcgcctcgtg aactcatctt cgggggtaga gcactgtttc 2520 ggctaggggg tcatcccgac ttaccaaccc gatgcaaact acsaataccg aakaatgtta 2580 tcacgggaga cacacggsgg gtgctaacgt ccgtckttga ararggaaac aacccaracc 2640 gccagctaag gtcccaaagt catggttaan tgnggaaacg atgtggaagg cacangcanc 2700 nnncngcccn gatgttngct tanaanccnc cntcntttta anaaagggtn atnnctcnct 2760 ggtnnantnc ncctnnncag aanattnang ggnctaaacc ntncnccnna nntgnnnnnn 2820 nnannctttt tctttntnga aagggnnccc ggannntngt anntagttnn ttgatnngtg 2880 ntngtgnttn aganggtngn ggntgccgna aggatnngag anngtannna cnatnncnat 2940 ngtgtggngt tanntntgaa anagaagagt tagcgtacng ngttngcgga gcanagnnga 3000 ntncttagta nntnnnanan agcgnngnag gnagatgnga tggncgnnta gtnnanattt 3060 gnnggngcng tgnntagagn nnngnntntt nggctntnan tnaaanaatn naggnatgtt 3120 agtgtgtgaa nnantgnnan angtantnat aannnnnnaa aganagagtn gnnnntncnn 3180 ctgnggnttt nnttagnggn ctngcannnn aaganttctt tgttaggtng nagnncnnna 3240 tnntnncagg tnnaggggaa ncntannnnn tgtttnccnc ncnnnnancc anaanantcc 3300 nnnntaagnt ttttntcggn gggannannn cncngggtgg gggannacgn cncntcngga 3360 ggnnggnnaa ncccnccccn nccacccaag gnncccccna taatcnttat tttngnngga 3420 aaatgtgnna nggganngnn cacccnagcn annaggttnt ggtntatnaa nnanccaccn 3480 ttnntttnaa aaaaagggta ntanggttcc ttgntgrant nngncctcwc nggaaaaant 3540 gnancggggt taaccnancc accaaagttw cgccaccgan cactatgtgt tgttgggtag 3600 gggagcgttc tgtaagcctg cgaaggnntt nngnanncct gtwagnngcg tnntangnct 3660 nnggaggtat cagtaagtgc gaatgctgac ataagtaacg tataaagcgg gtgaaaagcc 3720 cgctcgccgg aaggaccaag ggttacctgt tccaacgtta akwgggngnc agggtgagtc 3780 gaccmmsaag gccngrggca cgaaaggcgt agtcgatggg aaacaggtta atattcctgt 3840 acttggtgtt actgcgaagg ggggacggag aaggctatgt tagccgggcg acggttgtcc 3900 cggtttaagc atgtaggctg gttatccagg caaatccgga taatcaaggc tgaggtgtga 3960 tgacgaggca ctacggtgct gaagtaacaa atgccctgct tccaggaaaa gcctctaagc 4020 atcaggtaac atcaaatcgt accccaaacc gacacaggtg gtcaggtaga gaataccaag 4080 gcgwttkaga gaactacgga gtgaaggaac taggcaaaat ggtgccgtaa cttcgggaga 4140 aggcacgctg gtgtgtaggt gaagtccctg cggatggagc tgagaccagt cgaagatacc 4200 agctggctgc aactgtttat taaaaacaca gcactgtgca aacacgaaag tggacgtata 4260 cggtgtgacg cctgcccggt gccggaaggt taattgatgg ggttatccgt aaggagaagc 4320 tcttgatcga agccccggta aacggcggcc gtaactataa cggtncctaa ag 4372 <210> 18 <211> 5502 <212> DNA <213> Streptococcus mutans <400> 18 ntntncttan cncccggtnn nnnnntatna tttntntata taaaattgtg nncgaccacg 60 gttttttgta cngtagnnca anantctant antacantcn tatngaanac ccgtgttntn 120 annanncgng aattnncttn ntagaaangn gttntntaaa anaanccnnn ggggtanntt 180 nttntattag nngncccgtg gtaactncaa tnnntncggt aggcngcttt tgtcnnncca 240 atantgntgn gantnggaat antnntntnt tntnnnnngn gnggganntt ttatggggnn 300 caatncnggt ggggtgtttn ntanngggng nnnnagccan ncntttctta ancnnttccc 360 ancntttttn gnggcccncc ccnaacncaa gttggaagag ngggggagtt ttttttgggt 420 gagnntngcc tncanaaaaa aantnnnncc ggtcaagcga ataagcgcac acggtggatg 480 ccttggcggt cagaggcgat gaaggacgtg gcagcctgcg aaaagtatcg gggagctggc 540 aacaagcttt gatccggtaa tgtccgaatg gggaaaccca cccgcttgcg ggtatcctgc 600 agtgaataca tagctgctgg aagcgaacct ggtgaactga aatatctaag taaccagagg 660 aaaagaaatc aaccgagatt ccgtaagtag cgacgagcga acgcggacta gcccttaagc 720 tgatttggtt ctaggaaaac actctggaaa gagtggccat agaaggtgat agccctgtat 780 ctgaaagggc catttcagtg aagacgagta gggcgggcac gtgaaaccct gtctgaacat 840 ggggggacca tccttcaagg ctaaatacta ctgccgaccg atagtgaacc agtaccgtga 900 gggaaaggcg aaaagaaccc cggagagggg agtgaaatag aacctgaacc gtgtgcgtac 960 aagcagtagg agctccgcaa ggagtgactg cgtacctttt gtataatggg tcagcgactt 1020 actgttcgtg gcaagcttaa ccgtataggg gaggcgaagg ggaaaccgag tctgataagg 1080 gcgcatagtc gcgggcagta gacccgaaac cgggtgatct agtcatgccc agggtgaagg 1140 tgcggtaaca cgcactggag gcccgaaccc actcccgttg caaaggtagg ggatgaggtg 1200 tgattaggag tgaaaagcta atcgaacccg gagatagctg gttctcctcg aaagctattt 1260 aggtagcgcc tcatatgtat cctctcgggg gtagagcact gttatggcta gggggtcatc 1320 gcgacttacc aaaccattgc aaactccgaa taccgagacg gactgtatgg gagacacacg 1380 gcgggtgcta acgtccgtcg tgaaaaggga aacaacccag acccacagct aaggtcccaa 1440 attttgtgct aagtggaaaa ccatgtggaa aggcacagac agccaggagg ttggcttaga 1500 agcagccacc ctttaaagaa agcgtaatag ctcactggtc gagtcggtct gcggggaaga 1560 tttaacgggc taagcacaga accgaagctt ggggtgcata ctttgtatgc gcggtagagg 1620 agcgttccgt aagccgttga aggtggattg agaagtctgc tggaggtatc ggaagtgcga 1680 atgctgacat gagtaacgat aatgcgggtg aagaacccgc acgccgaaag cccaaggttt 1740 ccttgcgcaa cgttaatcgg cgcagggtga gtcggcccct aaggcgagga cgaaagtcgt 1800 agtcgatggg aagcaggtca ntntncttan cncccggtnn nnnnntatna tttntntata 1860 taaaattgtg nncgaccacg gttttttgta cngtagnnca anantctant antacantcn 1920 tatngaanac ccgtgttntn annanncgng aattnncttn ntagaaangn gttntntaaa 1980 anaanccnnn ggggtanntt nttntattag nngncccgtg gtaactncaa tnnntncggt 2040 aggcngcttt tgtcnnncca atantgntgn gantnggaat antnntntnt tntnnnnngn 2100 gnggganntt ttatggggnn caatncnggt ggggtgtttn ntanngggng nnnnagccan 2160 ncntttctta ancnnttccc ancntttttn gnggcccncc ccnaacncaa gttggaagag 2220 ngggggagtt ttttttgggt gagnntngcc tncanaaaaa aantnnnncc agccccccca 2280 nnaggnntgc caagcagnaa nggatgnntn ttttcattng gnaannagat naaagnggna 2340 acaagggcnc nntttnancn nnaannantn gacnacnnnn aancangttc tcangagtcg 2400 gggctttacg tcatngcggg tgtntcnaga atganaggca ncatngtaca attgtcataa 2460 nagtntnctc gntgngacaa caggntatan tcnnccgcca ctttttcgta cngnngggng 2520 gagntnngnc annganatat cgnnnncctt taggaccgtt atagtgtacg gcncgccgyt 2580 sactggttgg cttcaatgta ncnngcayct ataagccgnc ttacgcgtag agcatgctcc 2640 tcttaacckt cwagacamcr gktcaggtcg tctagcccct natacgttcn gccttacrkc 2700 ttytgcagag acnctcgtgt attttgyata aacawtcgcm tgggcctatt caytgcggmt 2760 ctmgtcgggc ygtgcaccct agnayagagc aymcctgtct cccgnancaa aaatgncnnt 2820 tnacggggtc attttgccga gttccttaac gagtatgttc tctncgatnc accttaggta 2880 ttctctcctn cgcctacctg tgtcggtttg csgtacgggg cacctctcac ctcgctagag 2940 gcttttcttg gsagtgtgga atcaggaact tcgctactat atttcgctcg ccatsacagc 3000 tcacngcctt acgggaaacg grtttgccta tttmccagcc taactgcttg gacgcggata 3060 tccaataccg cgcttgccct atcctcctgc gtccccccat tgctcaaatg gtganggagg 3120 tggtacagga atatyracct gytgtcccat cgmctacgmc tttcgksctc gscttaggks 3180 ccgactmacc ctgmgcsgay kaacstttsc kcaaggaaaa ccttrggcwt tcngngcgwg 3240 cgggwtyytc anncccstca ttatcgytac tcatrycrgc attckcactt cygatagcgc 3300 tccagcgagw ccttctcrrt ctcrccttca acrgccctta cggaacgctc ctcctaccac 3360 tggttcgcat aasaacagtt atgcacscca agccttctgt gwtacygtkc ttaggccccc 3420 ggttamatyt tycsgcgcas accgtnacct cgrcccagtg agctattacg ctyctcnttt 3480 aaanggngtg gctgcttcta agaccaacmt ccctggytgt ctgtaaagca actttccaca 3540 atrstttttc cactttagca caggaatact ttngggacct twgccttgct gggtctgggg 3600 cttktttccc ttttmackam gggatckttw gcacctcggc cgtgctgwcn ntcccaaggg 3660 gtacagatcc ktctcggyan ttyggggagt ttgcaactgg wattcggkaa gyccggcgrk 3720 grgcccccta gycatnnana cagtgctcta cccccgagag gatacatatg aggcgctacc 3780 taaatagctt tcgaggagaa ccagctatct ccgggttcga ttagcttttc actcctaatc 3840 acacctcatc ccctaccttt gcaacgggag tgggttcggg cctccagtgc gtgttaccgc 3900 accttcaccc tgggcatgac tagatcaccc ggtttcgggt ctactgcccg cgactatgcg 3960 cccttatmag actsggtttc cccttcgcct cccctatacg gttaagcttg ccacgaacag 4020 taagtcgytg acccattata caaaaggtac gcagtcactc cttgcggagc tcctactgct 4080 tgtacgcaca cggtttcagg ttctatttca ctcccctctc cggggttctt ttcgcctttc 4140 cctcacggta ctggttcact atcggtcggt cagtagtatt tagccttgra ggatggtccc 4200 cccatgttca gacagggttt cacgtgcccc gccctactcg tcttcactgr aatggccctt 4260 tcagatacag ggctatcacc ttctatggcc rctctttcca gagygttttc ctagaaccaa 4320 atccagctta agggctagtc cgcgttcgct cgtcgctact tacggaatct cggtatcgca 4380 tttgctttct ccttgctccg gktacttaga trtttcagtt caccmggktc tgccttctca 4440 kcakcctatg wattcactgm tatggatact gctcgcatta gcgagcagtg ggtttcccca 4500 ttcggamatc taccggatca aagcttgctt rccagctccc cgatagcwtw tcgcagtgyt 4560 cgtccacgtc cttcatcgsc tcctgasygc caaggcatcc accgtggysc stcttatstm 4620 rncttraccg tcwaaaagga atcactacgt ggatatcctt gcattacaat tgaatgngna 4680 tgtctactnt cgtnntanct agttaacaaa sancncgctw tgnaggaatg mtccttmana 4740 actmaacaag atnccraccg ctcctgntnt atacgaccgs gaggtctant attccgtata 4800 ancntcckwa gagaggaggt gatncmgccs cnccttccga tgcggcttcs ttgctacnac 4860 ttcacccaan natcatctgt nccacnnctk cggcggstgg ctcnanaana ggctacmtca 4920 ccgactgcng gtngcnacna nantnntggn ggnncgacng gngngtncga acaanatnct 4980 tannaaagan gnnatggcgc cgnncttccg ntacagctac cttannncaa nntcacccgn 5040 aannnctgnn ccntncntcn ggnnacnngg cttccaaaaa ngggtacnnc aacngannnn 5100 ggnnagcgnc nnanntncna cgggggggnn nnnatccngn gacggngcnn atacncatnc 5160 nnaaanannn aannncaccg ntangnnntn anngnagngn nnnnnnngat agtncncacn 5220 ncgananann annncnanac ccccnnnnca antatannnt nnacncannn aaaccnaann 5280 aantnaagnt ttnacncgnn nnnaannnna ccnccnnnnn ncgcngcaan nacnannann 5340 ncnnaagnnn annnnngaac nnnttngang tannangtnt nccnnnncca ngngnntgng 5400 ncatnnngcn ncnnnggcnn gagnnggngn nnnnncactn ncnactntta nngnngnngt 5460 aanttnnntc annnnnntan nngggncttn annnnnnacg cn 5502 <210> 19 <211> 4488 <212> DNA <213> Kingella kingae <400> 19 gggcngganc cccccccttn ttannnaaaa gaagagatng ttagggtncn cccccntatc 60 ggnnanttag atgaagatgc gaagagcnaa naagnacnca anangggttt tgtagctcag 120 gtggttagag cacacgcttg ataagcgtgg ggtngtaggt tcaagtccta ccagacccnc 180 caggattaga gaagcgaatt caagttgtaa agcttacgag aagtaagaaa tactgggggc 240 atagctcagt tggtagagca cctgctttgc aagcaggggg tncatcggtt cgatcccgtt 300 tgcctccacc aagattaaat aaaatgaaaa attagattgc aaattaaagc aagtttagat 360 aaactggcga gcttacttta atttgcgata tattttttat aagcgaaagc tgagaagaag 420 tataattaaa cgcatcgatc tttaacaaat tggaaagccg aaatcaacaa acaaagacaa 480 tgttgtcgat ttggtttggg attgctnngn atnnnnangc aacagtattt caggcagcct 540 gcaaaggcaa caaatcgaat aacaaaattt gggtgatgat tgtatcaact aatcttgaat 600 tcaaaaggca aggttagtac acaacaagca gtaagcttta tcaaaagtag agaatctaag 660 ttattttagt agtcaacgct aaaatagcaa agtcagaagg ttcttcaaat wataggagtn 720 caagtwaata agtgcaycat gtactttgat tcaatgcgat gtggggacgg agaaggttag 780 gttagcaaac tgttggaata gtttgtttaa gccagtaggt ggaaagagta ggcaaatccg 840 ctctttctta acaccgagac gtgatgacga gtgtctacgg acatgaagtg accaatacca 900 cgcttccagg aaaagccact aagcttcagt tgaatcagaa ccgggtggat gccttggcga 960 tgataggcga agaaggacgt gtaagcctgc gaaaagcacg gagaagctgg caaaaaagca 1020 atgataccgt gatgtccgaa tggggaaacc cactgcactc gtgcagtatc ctagtctgaa 1080 tacatagggc tagagaagcg aacctggaga actgaaccat ctaagtaccc agaggaaaag 1140 aaatcaaccg agattccgca agtagtggcg agcgaacgcg gaaaagcctg tatatgataa 1200 tggttaaaga tagaaagaag ggattggaaa ncttccgcca tagtgggtga tagccccgta 1260 tttgaaatty ttaaccatgg tactaagtat acgagaagta gggcgggaca cgtgaaatcc 1320 tgtctgaata tggggggacc atcctnccaa ggctaaatac tcatcatcgn accgnatagg 1380 tgaacccagt accgtgaggg aaaggcgaaa agatnacccc cggaagggga gtgaaataga 1440 acctgaaacc tgatgcatac atacagtggg agcatctttg tggtgtgact gcgtaccttt 1500 tgtataatgg gtcaacgact tacattcagt agcgagctta accgagtagg ggaggcgtag 1560 ggaaaccgag tcttaatagg ngcgactagt tgctgggtgt agacccgaaa ccgagtgatc 1620 tatccatggc caggatgaag gtgcggtaaa acgcattgga ggtccgaacc cacgcatgtt 1680 gcaaaatgcg gggatgagct gtggataggg gtgaaaggct aaacaaactc ggagatnagc 1740 tggttctccc cgaaaactat ttaggtagtg cctcgagtat gagactgatg ggggtaaagc 1800 actgttatgg ctagggggtt attgcaactt accaacccat ggcaaactaa gaataccatc 1860 aagttgctcc tcgggagaca gacatcgggt gctaacgtcc ggtgtcaaga gggaaacaac 1920 ccagaccgcc agctaaggtc ccaaatgata gattaagtgg taaacgaagt gggaaggcac 1980 agacagccag gatgttggct tagaagcagc catcatttaa agaaagcgta atagctcact 2040 ggtcgagtct tcctgcgcgg aagatgtaac ggggctcaaa tctataaccg aagctgcgga 2100 tatacttttg tatatggtag gggagcgttc tgtaagcctg tgaaggtgac ttgtaaaggt 2160 tgctggaggt atcagaagtg cgaatgttga catgagtagc gataaagtgg gtgaaaagcc 2220 cgctcgccga aagcccaagg tttcctacgc aacgttcatc ggcgtagggt gagtcggccc 2280 ctaaggcgag gcagaaatgc gtagtcgatg gaaacaggtt aatattccnt actttattca 2340 atgcgatggg tgtnanttnt tannggnnnn tgtgttnnta nannnnatnn nttngggtnn 2400 nannnnnagt taatgntnga ttantgggag tgntatntan gnaaattann ntttgnnnag 2460 atgnannnna tnnncgtgnn ntannaacng gtcaanttnn ngangcangt nanantaatg 2520 agnnaggnaa nngnnatagn gcnngnagaa gcgggnggtn nnnnnntnna nnggaananc 2580 nngggnttan cgtngtgaag tggtggnngt tgntnntntt nnantattnn gtaatgngnn 2640 annngganna ggngngtnga ngngnntann atntnanaaa aagaananan angnnggtan 2700 tagnnatngg ntgnaattan tnnnagggng gatngtnngt nnattgntgg ttngtanatn 2760 tntnnagnnn ttatggnagg angntganaa gattnnaaan anntnaaagt nttntgatnt 2820 gnatnggngt nngtnnnnnt ganntttann gtnaggtagn nantntngga nggnnngnng 2880 ggtnnaaggt tangnangnn ngttgttnnn annnnggggg gnggntggna gnnangttta 2940 atttttantn gnntnatgtt gggggnggtg ngngngnggn nnnanntagg ggnaagggcc 3000 caagtanagt cantatgngt naaggagcag ngttaatatt cctgtacttn tgattcaatg 3060 csatgtgggg acggagaagg ttaggttagc naaactgttg gaatagtttg tttaagccag 3120 taggtggaaa gagtaggcaa atccgctctt tcttaacacc gagacgtgat gacgagtgtc 3180 tacggacatg aagtgaccaa taccacgctt ccaggaaaag ccactaagct tcagttgaat 3240 cagaaccgta ccgcaaaccg acacaggtgg gcaggatgag aattactaag gsgsttgaga 3300 gaaytmggga gaaggaactc ggcaaattga taccgtaact tcgggagaag gtatgccctc 3360 taatgttaag gacttgctcc gtaagcatct gagggtcgca gagaatcgna gtgngctgcg 3420 actgtttatt rwaaacacar cactctgcta acacgaaagn tggacgtata gggtgtgacg 3480 cctgcccggt gctggaaggt trattgaaga trtgcncaag catcgratmg aagcctccag 3540 taaacggcgg ccgtanasta taacggtnct naagggangg nnatncctnn ttnggtaant 3600 tcggaccccg cangaanggc gtaacatatg gccacncatg tntcctcccg agactcagcg 3660 aagntgaaat nnttgtgaan atgcaatact cnccantgnn ggttagaaag acgccgtnan 3720 cctttactgt agcnnngcan tggacntngn agtcacntgn gtngtatagn ngggaggntt 3780 tnaagcagac gacgtccagt cnctgtggag accgtccttn aacanaccat cccnggcgaa 3840 gttncaggtn gctaacccag acccnttacc cggntccggg gncccnagca accagtntnn 3900 nngnancngt ccttnaannc cncccnngnn nantttaggt tnnaacccag acccgttatc 3960 cngggtcggg gaccgtgcat ggtaggcagt ttgactgggn cggtctcctc ccaaagagta 4020 acggaggagt tcgaaggtta cntaggtccg gtcggaaatc ggactgatag tgcaatggca 4080 aaaggtagct taactgcgag accgacaagt cgagcaggtg cgaaagcagg acatagtgat 4140 ccggtggttc tgaatggaag ggccatcgct caacggataa aaggtactcc ggggataaca 4200 ggctgattcc gcccaagagt ccatatcgac ggcggagttt ggcacctcga tgtcggctca 4260 tcacatcctg gggctgtagt cggtcccaag ggtatggctg ttcgccattt aaagtggtac 4320 gtgagctggg tttaaaacgt cgtgagacag tttggtccct atctgcagtg ggcgttggaa 4380 gtttgaaggg ggctgctcct agtacgagag gaccggagtg gacgaacctc tggtgtaccg 4440 gttgtttcgc cagaagcata gccgggtagc taagttcgga agagaaaa 4488 <210> 20 <211> 3177 <212> DNA <213> Bacteroides ovatus <400> 20 atnnngnggn nnntnnnnnn nnnnnangcc cccnnncnaa ncncnnnaaa aaacncnggg 60 yccctraagg tacgttaccs carraaagck tcctangggt aaaactggta attggggsta 120 aagtsgtaac caaggwagcc ggtmccggaa aggtgcggct kgtaamaggn ccyccntttc 180 tggaagcgna atgccggtaa ctgtcmcaaa aacgtggnca ngccacctaa aaagmcttta 240 aanggtgtcn tgttttttnt gtactanctg gtacttgttt rtttatnana catatagatc 300 amccatctat tagaagtata gatagagata amcaagagaa aaagaagccg agtctagacg 360 aaaggtagac aaggttgaac tagtcctata gctcagttgg ttagagcgct acmctgataa 420 tgtagaggtc ggcagttcaa ctctgcctgg gactaccaag ctcaagaatg aggaacagcg 480 aaagctaaaa gctaaaaaac aaaattcttk attaacctcy ctggggngat tagctcakct 540 ggctaragca tctgccttcg canncgcaga ggngkcaacg gttssaatcm gtnntrttct 600 ccacgatctc tknaaaanag agaaacsact cttctkwcat gatgtacraa aagtagaaat 660 tttagtaaga gctaraagta tatatcaamc cgymcgtgtt cgagtatgca atattgatag 720 aaggaagcat tccwctacca ayaccagtas gcagaaataa aacgtttgwa agtaaagtaa 780 gcaagggcgc atggcggatg ccttggctst cgkaggcgat gaaggacgtg ataagctgcg 840 ataagcttcg ggcaggtgca aataaccttt gatccgaaga tttcygaatg ggacaacccg 900 gcattctgaa ggaatgtcat ccatcctaga tggaagctaa cgcagggaac tgaaacatct 960 tagtacctgt aggaaaagta aaataataan ntgattccct ngcnngtagt ggcgagcgaa 1020 cggggwttag cccaanccaa tgttgttacg gcaatattgg ggttgtagga ccacgatgtc 1080 gcaagaaatt tagtgagaag aaccctttgg aaaatgggac catagacggt gatagtccgg 1140 tattcgaagc taaatgaagc gtagtggtat cctgagtagc gcgggacacg aggaatcttg 1200 cgtgaatctg ccgggaccat ccggtaaggc taaatactcc cgakasaccg atagcgaacc 1260 antactgtga aggaaaggtg aaaagtcact tnccgnaata gcaagcagtg aaatagtccc 1320 tgaaaccgtg cgcctacaag ncggtcggag ctgtgtaaac agtgacggcg tgccttttgc 1380 ataatgaacc tacgagttac tttttccggc aaggttaagg atcttgagat ccgcagccga 1440 agcgaaagcg agtcttaaca gggcgattag tcggaaggag tagacgcgaa accaagtgat 1500 ctacccttgg tcaggttgaa ggttaggtaa cactaactgg aggaccgaac cgataagcgt 1560 tgcaaaagct tccggatgam ctgagggtgg gggtgaaagg ctaatcamac tttgkagaan 1620 agnytggtac tncnccgaaa tgcatttaga gtgcntgncn ttgganaant tnctanctgt 1680 gangnnnnnc nctganannt ncggggcntn ccnnnggnng ggttatatnn ctntnnngcg 1740 agggncntnt anncccnggc nnnngnnnng gnnnnaacnc ccnnntnngg gnaanaaann 1800 ntnntngggg ggtccccnnt wtwttnntan ntncagaana angnatnnat tncntaatan 1860 cannnnngnn gngnttttgg cannnctann cgtcnantnt tncttaaaat nnnaannnan 1920 tnccatanca nnantnntnn tnnttccnnn anaantatta tnntanttac tttnnantat 1980 ttnnncttna ngaatatnat tattcttnan nanttanntt ttantcnntt tttcnnnnnn 2040 ctanatngnn nnannanatt aactcnaatc nnnnanannt aactttaaan atctantaca 2100 tncnannnct anganntatt natanttntn tntgtttant tgnnntnntn ncntcttgnt 2160 ntncnacttt tttantannt ttcntancct ncanntncta nngtnnttan tncnntntcc 2220 nttattannn ttanaangcc aanacatnat cannntctat tnctnaccat catntcttna 2280 tcnnactnac tnctgnnttn atctaccatn cccgaattag gtacccaggc aatgcacctg 2340 gcggcacaac tggtaaacca gcggtcagtc caacacggtc ctctcgtact agtgtcagag 2400 ccacgcaaat ttcatacgcc cacgatagat agagaccgaa ctgtctcacg acgttctgaa 2460 cccagctcgc gtgccacttt aatgggcgaa cagcccaacc cttgggacct tctccagccc 2520 caggatgtga cgagccgaca tcgaggtgcc aaacccctcc gtcgatatga gctcttggga 2580 gggatcagcc tgtntatccc cggagtacct tttatccttt gagcgatgtc ccttccatac 2640 ggaaacaccg gatcanntat gctctagttt cctacctgat nnanttgtct gtctcccant 2700 caagcgccct tatgccattn cacntntacg gacggatacc caatcngttc tgnagggnnc 2760 nctttagaan tcctncgtct anacttttgg nggncnttcn tcccatntaa nancnttcca 2820 ccaaaaagnt gtttctatcn tnttacnagt ttggaacttt taantattta naagggntng 2880 nnagtttant ctatcnnnan tcnnnnnaat tctttggnan cctanttttt ttnnaaanan 2940 tttnnggnnt antnntgtan tnttnagtct ncccnaattn tttnctnttt ntaacgttat 3000 antgttnata gggtnntcat nnngntnntt ntgcgngntn tnttntntnn nnngnatttt 3060 nntnnactnt ancnntnncn tnctaccatc tnntcttntt tctntcnagn ntnnttttta 3120 ttctnncacn aaccttcntc tccgntttnt ttctntnttt tncnnctttc ctccact 3177 <210> 21 <211> 2770 <212> DNA <213> Bacteroides thetaiotaomicron <400> 21 ggggggccnn ccnatnnggt ncctttttaa nntccnnaag gccncnantg ttttantncc 60 nngaaanaan nngcattngg gnnaangnnc cnntnccnaa aanaaaagnn tttggnnncn 120 ccngcccgtc gagccctnaa agccgcgggg tactgaaggt aggtaaccgc aaggagngtc 180 ctagggtaaa ttntggnaat tggggcttaa gtygtnntca nanggtagcc gtaccsgaan 240 ggtgsggctg gaacmccctc ctttctggag cgnatgtsgt tacaacgatt tcatrggttt 300 ctattattgt actactggta cttgtttatt tataatatat agatcamcca tctatagaag 360 tatagataga gataamcaag agaaataaag aagccgagtc taacatcagg tagacaaggt 420 tgaactagtc ytatagctca gttggttaga gcgctacact gataatgtag aggtcggcag 480 ttcaactctg cctggganta ctccgaacaa aaattgtcgt tgccaataca gtgttggaat 540 gttcaattac tcttggggga ttagctcagc tggctagagc atctgccttg cacgcagagg 600 gtcaacggtt cgaatccgtt attctccacc atctctgaaa agagaaacga tctttgacat 660 gatgtacaaa aagtaaaatt tagtaaagag ctaaaagtat atatcgaacc gtacgtttta 720 agtacaacac tattaagtac tttgggttaa tacctgatac ttgataccta atacttaaaa 780 gtaagtttga aagaaagtaa gcaagggcgc atggcggatg ccttggctct cggaggcgat 840 gaaggacgtg ataagctgcg ataagctctg ggtaggtgca aataaccttt gatccagaga 900 tttccgaatg ggacaacccg gcattctgaa ggaatgtcat ccatctttga tggaagctaa 960 cgcagggaac tgaaacatct tagtacctgt aggaaaagaa aataataatg attcccctag 1020 tagtggcgag cgaacgggga atagcccaaa ccacccatgt tacggcatgt gtggggttgt 1080 aggaccacga tgtcgcaaga catttgatga gtagaatcct ctggaaagtt gaaccataga 1140 cggtgatagt ccggtatacg aagtcaaatt aagcgtagtg gtatcctgag tagcgcggga 1200 cacgagaaat cttgcgtgaa tnctgccggg accatnccgg taaggctaaa tactncccga 1260 gaagnaccgn atagcgcaca cccaagtact gtgaaggaaa ggnntkwaaa scmctattcg 1320 aatagcaagn agtgataata gtccctgaaa ccgtgccgcc tacaagtcgg tcggagctgc 1380 ttaagcagtg acggcgtgcc ttttgcataa tgaacctacg agttactttt tccggcaagg 1440 ttaagcatct tgagatgtgc agccgaagcg aaagcgagtc tgaacagggc gtcgagtcgg 1500 aaggagtaga cgcgaaacca agtgatctac ccttggtcag gttgaaggtt atrgtaacac 1560 taactggagg accgaaccga tawgcgtatg aaaagcttac cggatgtaac atgakggtgg 1620 gggtgtaaag gmtaatctaw actatrkagt atagtmtmgt actccccgat atatrcatat 1680 atakrtryag ccmtatrtga gtatactata trtgakrtak agckactgat amkatgckag 1740 ggctatcacc gymtatcata gtactntgat atatactccg atatgcgcat ntanttmtat 1800 cacatakagt gatggcatgg rtgmtatata gtccatrtac tatatagkak atakatatnc 1860 ataccntcan ctataggtcc cyagwatawa yastnagtat gnactatact atagwcannt 1920 atatgmtata tacngctnng atrtatagct atggatagya ncctatatat ayatatatak 1980 atrcrtayan ctcactatrt cnnggagtat atggagtgga tactatatat cnggcatata 2040 tantrtatat atacckatat ctatgggatn nnnnatgatn ggnanggtac ntatmccata 2100 tatatatata tatatatata tatatatata tatatatata tatatatata tntncatata 2160 tatctatata tatatatnat atatanctat atatatatat atatatatat atatatatat 2220 agtatatata tatagtanat atattatata tanagtgtat agnctaccat atatatatan 2280 gatagactat atatatntnc ntacanatat atanntatnt ntctananan atgatatnnc 2340 natanngcnn tananannta natatanang tnnatcaggn agtatannag agcatatntg 2400 atccgntgat tccnatanan angtacatng ctcnatagna tatatagnat actccgggna 2460 tatcngtctn tatccctccc atagagntnn atatatcgaa ngggttnggc acntngatgt 2520 cggcncgtcn cancctnggg ctggagaagg tcccaagggt nggnctgttc gcccattaaa 2580 gtggcacgcg agntgggttc agaacgtcgt gagacagttc ggtctctatc tatcgtgggc 2640 gtatgaaatt ngcgtggctc tgacactagt acgagaggta ccgtgttgga ctgaccgctg 2700 gtttaccagt tgtgccgcca ggtgcattgc ctggagtatn ctggagttcg ggtangcgaa 2760 aaaagagnnc 2770 <210> 22 <211> 3455 <212> DNA <213> Clostridium difficile <400> 22 gngnnccnnc nnncnngccn ggggggggnn nccccccncc nncgagncnn tcnnccnncn 60 nncngnggcc nttnttannn gggggggncc cccnngnngg ggnggnnngn annccttcnn 120 cggggggggg ggncagcccc cncntnnnnc cggcagnnng gncccccaaa aantngtnan 180 tgnggngnaa naaggggggg ggggccncac cnaccccccn ggngtttgtt ggtttaaccn 240 cnccccccnc ttccntcaan aggggggcga aagnancccc ngngcnangg ggtccccccc 300 nccgcagaat ttncgngggg gggntaattt cttaccccnn ggagcngnng gcccccagta 360 nanggngggg gggggcggct ccccacgggg tgnngnngca nccccngnnn gcctcangtg 420 cacagtttgg gngngagaaa tggggcncnc gagnagcnna tgntgggggg ngaggncncc 480 cncngccngg acncncnggg ttnccnggnn angntgtccc ccccccntgg ggttagncgg 540 ccccnnaagc tgngcccnaa nggcggtagg tgatggccan cnggtnaata ntccggnacn 600 cccgntanct gtntgcnagr rgnngsagta nntcncnnaa ggnnnagccn tatnagnanc 660 ngnananana tcncnnccng ngttnnnagk rgrayyccnc sctgcgnnca atagcangtg 720 nngatnccnc gcaattnatt tcngttggcg cntngcgtta gcactttgaa aactgcatat 780 atatttagtg atatgacatc taatttgtaa tatataaagc tgataacttt tttaaaatta 840 wcgaangttg atrgcttctn aatctatcaa cnaccntttt taaytggntc aagttattaa 900 gggbgcaggg cggatgcctt ggcactangg arccratgaa ggamgtkant aacgytgcga 960 nntaagcttt cggggagttg camgtwawct ttgatcncga aagatttccg annatgagga 1020 aactcacttn agangtaatg tstaagntat cattaagtra atacatwgct taatgagngg 1080 gaastcaggg aactgawaca tnctaagtac ccgangcaag nanaaagaan tcgnannacc 1140 ganttcnttc ngtaagnang ngngaangan gangcggctt agccccaaac cnaakaaagt 1200 tttgctttat tnnggggykg cnggatcata tcataacgaa gnanggtwtc gtaattgaag 1260 aggtttggww agacccaccm cascaaggta akagtcctgt atgttaaacg agaagacttt 1320 agatatgatc cagagtacca cgggacacgt gaaanccctg tssgaagcag gagggaccac 1380 cctccawggc taaatactac ctagtsaccg aatagccgta tagkaccgtg agggaaaggt 1440 gaaaagaacc ccgggagggg agtgaaatag aacctgaaac cctgcactta caagctgtgg 1500 aagcacattt cttgtgtgac cgcgtncttt ttgtanaacg ggccaacnan ttacnttaag 1560 tagcaaggnt aatcncttaa ggtgcgganc ctnagcgaat ncnatttttt nactgaccgt 1620 tcacttactn gnccnngact cnnnaccggn ccatctnnnc atgnncatag atcnnaatta 1680 nagnnnntcc ccncnctggt cctanctntt atttnaagag tgcgtaatag ctnactggty 1740 ggagtgatcc tgcsccgaag atttccgggg stwtaaactt tactaccgaa agctacgggc 1800 atcagtaatg atgggtaggg gagcttcccn atacgggttg aagcatgacc gtaaggacat 1860 gtggacagta tgggagtgag aatgttggca tgagtagcga gatgtgggtg agaatcccac 1920 aggcccgtaa acccaaggtt tccaggggaa ggttcgtccg cccctgggtt agtcgggacc 1980 taagctgagg ccgaaaggcg taggtgatgg acaacaggtt gatattcctg tactaccgat 2040 aaccgtttga ragaagggat gacacagtag gataagctaa gcacactgtt ggttatgtgt 2100 gcccaagcat tgaggcagtc aaagtaggca aatccgcttt gataatgctg ggatgtgatg 2160 gggagcgaaa tttagtagcg aagtagctga tttcacactg tcaagaaaag tctctatcga 2220 ggttaaaggt mcccgtaccc gcaaaccgac acaggtgggt gaggagaktw tcctaaggcc 2280 agcgagagaa ctgttgttaa ggaactcggc aaaatgaccc cgtaanngnn ncttagggat 2340 aaggggtgcc accatgcagg tggcccgncc agakaantwg gcccwasccg actgtttacc 2400 cnaaaaacnt tnngnttnat nntnnnnnnn nnnnansgnn ntnttncann tnantgcctw 2460 agtncgcaag acgatgtata ggagctgacg cctgcccggt gctggaaggt taaggggatc 2520 tgttakagca atcgaagcag tgaacttaag ccccagtaaa cggcggccgn nactataacg 2580 gtcctaaggt agcgaaattn cttggcgggt aagttccgac ccngccacga aaggcgtaac 2640 gatttggggc acttgtntca acaaacatta ctcggkgaaa attgtaattc ccggtgaaga 2700 tgccggnata ccttgcgaca ggacggaaaa gacccccatg gagctttact gwakgcttga 2760 cattgggtct tggtactaca tgtacakgat aggtgggagg ctttgaaacc casgacgcca 2820 gttttggcgg agccatcctt gggataccac ccttgtagtr ctgggactct aactcatasg 2880 ccatgaatct ggtcttggga cactgtcagg tgggcagtnt atgactggkg cggtcrcctc 2940 ccaaaakgta acggaggcrc tcaaaggtts tctcagtacr rtackgaaat cgtacgtara 3000 gtgtmaaggc ccaaatagrg agctatgrtg tgcmagacat cnancatgtg tgcgagcgan 3060 argatgaaaa tcggacctcc ctrgkgrtcc tggycggtct cttgcgtgga agggnnngcc 3120 rkccgcttcm mcsgataaaa gacwatccyt ggcggataay rggcttttta yacycccccc 3180 nnaagagtcc gaacatnctt grgcnngggg rggcnanaaa tttggncacc atcgatgtcg 3240 gctcatcaca tcctggggct gtagtaggtc ccaagggttg ggctgttcgc ccattaaagt 3300 ggtacgcgag ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcaggcgt 3360 aggaaatttg agaagacctg tccttagtac gagaggaccg ggatggacgt acctctgtgt 3420 accagtgtcc tccaagggca ggcnggagct atncn 3455 <210> 23 <211> 2651 <212> DNA <213> Haemophilus aphrophilus <400> 23 ccngncncnc cannnctggg agtgggttgt cmccgmagya gatagcttaa ccgcaawggg 60 ggcgtttacc mcggtatgat tcatgactgg ggtwmagtcg taacaaggkr accgtagggg 120 aacctgcggt tggrtcmcct ccktaccgan aagacgaacy taagtgtccm cacagtttgw 180 ctgatgattg tagacaagaw agaacaaakc saangngtan nawncnaacn aagntntnna 240 gagcatcttt atatgttgtc cccatcgtct araggcctaw gnacatcgcc ctttcmcggc 300 ggtarccggg gttcgaatcc ccgtggggac rccatataaa gatgattayt catcttcttt 360 ataaaacctg ttctttaaca aaccggaaac avgctgaaaa cgagactttc aagaaagtct 420 gagtgakagt gattgataaa aggtgattac tcttaatama ttaactgtya ctgcttagcr 480 dcdttvvgtg tttayycrat tcaatgttgt gattttaakt ggattytcva tttaatgtta 540 agytcatkat gttgagttan cnatcataag aatacttgag gttgtnatgg ttaagtgaca 600 anagactgtv caattttkgt graatgncnn tcnttkkcaa tcagaggcgt catagttaag 660 gacgtgcncc nnttaatnct gccaagnnan naannnatnn nnnagnctnn nnntnnnngg 720 annnnntgag tncncnnnnn cgncatanag annntnnncg gccccnctgt nnnnttaatc 780 caagatgtcc gaatggggaa acccagtggg tgaagaaccc actatcatta tctgaatcca 840 taggataatg aggcgaaccg ggagaactga aacatctaag taccccgnag caaaagaaat 900 caaccgtaga natctttttt aaannnnctt ttttttnnnn nnccngnnan nnnnnnnnna 960 nnnnnncttn naanaanntt tnnnnngaat gnatnnanaa ggtnantnnt cttnaatnaa 1020 ttnnngntnc nnannancgg ngattanttt ttttnnannn angtngtgat ntttagaggn 1080 tnttncaant nannngtngn tnatgatgag tanntnatna naanaanncn tntggtttnn 1140 ttgnttaang angcagnnna cnaggnngcn tcccntgnca tangagnaga agaangnngt 1200 gatnntntna nanaangttt gantagatna taagaggngt tttnnncaag angncnngan 1260 ggggcncacc nggtggngaa cccnccnnca tnattttnnt cnancntatn atnatgngnn 1320 ccnccaggan taattcatcn nantannccc cngannanaa atcanccnnc nntttnttan 1380 tagnngcgag cnnacganaa gnccgtaggc atcctgttag tgataatgac agsagnacag 1440 aaggcaacaa sctgggtrag cttgnntgcg acacagggca tgakagcacc cgymctcgaa 1500 gtccaggctt akggtastaa gctaacgaca agtaaggcrg gacacgtrat atcctgtttg 1560 aakwtggggg gaccatccyc cmaggctaaa tactacctgn attsaccgat antagnaacc 1620 rgwacctgtg aaggaaaggc gaataagtaa ccmacggtga ggggagtgaa atasaacctg 1680 awaccttgta cgtacaagac agtgggagcc tgaaagggtg actgcgtacc ttttgtataa 1740 tgggtcascg acttatattt tgtagcgagg ttaaccgaat aggggagccg aagggaaacc 1800 gagtcttaac tgggckagta kttgcwaggt atagacccga aacccgktga tctagccatg 1860 ggcaggttga aggttgggta tacactaact ggasgaccga accgactaat gttgaaanna 1920 attagcggat gactcntgtg gctgggggtk aataggccaa ycwaaccggg attkatagcc 1980 tgnttcntcc yygaaattct atktakgtaa anccctttgt ataramamct ktcggggggt 2040 naaacaccct gntntcyggy ttaggggntn cntcatcmcc ktctnttmcn cnataaaann 2100 ncgttntncn atattttgnt cnngaacnan cnntnnttnn tntcntntgn tacanattnn 2160 nnntaacncn cnnttatttt tnttnttnnc tctnntttnt tnnnttnacn tggganacac 2220 nccnganggg tncncnacan ttnnttngtg tgnnnaaggg gaaaccnncc ccactaccng 2280 ntaaanttaa ngtnccnaaa annctttata tntnnantnt tcaanancaa aannattnat 2340 nctnnnnact ntgtttttnn tngttnnacn tnaanannan caaananaca ctntctnnag 2400 antanntnnn ttnntnnnac ttnantcntt ncncccccnn ananacnnnn tananantna 2460 nactnattnt tccnncanat atnnngntac cnnnaaccnc ctnnncnnat tngtanncnn 2520 tnnnntnana tngntnncna tcacntnnnc tnannttnan agagtancga ccaanactnc 2580 ntnctctaca tctnanctct actcnntccc ncccaatacn nncctacnnn ttcttatnaa 2640 cntacncttc n 2651 <210> 24 <211> 3077 <212> DNA <213> Neisseria gonorrhoeae <400> 24 ggntntgngt antgncaaan acnnncctcn agngtgnnnn gnattattat atgtccngnn 60 agaantgaaa cagngngggg nnnngngatn angtanttaa tngntgctnn nggcgngntn 120 gtnnatgnaa agngnttncn gngacancat aactgtnact attnctatnt tgcgcnctan 180 tggtgnagtt tatnngngna gttannnttn gngaagaann tccntnttaa ggannggnaa 240 ttatnnccta tnantngnnt acagtngttt ntnttttccn annttnncgg tnnnnncang 300 nanaaatnnt ntgannnctt attnaaattg gngccnttnn gnnaaannan tncnncngng 360 ttttataana gnnncgttng ttagnagngg ntantctnnc ctannagtnn gcgggntnna 420 ggttccncag acntatnnaa tatcccncta naaccnagng gnnnttgccn nntcgttngn 480 tcannnggnt anannnannc gcttnatnan nnnngggntn gnaggttnna ntccntcccn 540 nnccccncca anannngggg catagntcag tntggtnngn agcaccngct ttgcwagcag 600 ggggtcmtcg agtwcgatnc cgtttgcctc cncccnaaac tttacaaatg aannagcaag 660 tttgctgttt ttagcagctt attttngatt tntgcnngat agctagawta nacgacgcga 720 tmkatctttg ancaaattkg acaagcgcgm watcarcamc acamagacaa tgagtttgtt 780 ttgmtttttt attctttgca aaggataawa acctctcgca agagaaaaga ammgcaamca 840 tagtatttgg gtgatsattg tatcagactt aatcctgart acacaaaagg caggattaag 900 acacaacaaa gcagtaagct ttatcaaagt agggatttca agtttgctta cttagtcaac 960 gggtaggtaa acgcaagtca aagtaagttc ttsaaatsat aggagttcaa gtsaataagt 1020 gcatcaggcg gatgccttgg cgatgatagg cgacgaagga cgtgtaagcc tgcgaaaagc 1080 gcgggggagc tggcaataaa gctatgattc cgcgatgtcc gaatggggaa acccactgca 1140 ttctgtgcag tatcctaagt tgaatacata ggcttagaga agcgaacccg gagaactgaa 1200 ccatnctaag tacccggagg caaaaggaaa tcaaccgcag ntaccgcaan nggggntnna 1260 atnnanancn naanntncgn annccgnttt nccggcatgg tagggnagcg ttntgtagnn 1320 tnannaaggt gcattgtaaa gtgtgntgna ggnatcanaa gtncgaatgt tgacatnagt 1380 anngataaag cgggtnaaaa gcccgctcnc ngaaagccca aggtttcnta ngcaacgttn 1440 atcggngtag ggtaagtcgg cccctaaggc gaggcagaaa tgcgtagtcg atgggaaaca 1500 ggttaatatt cctgtacttg attcaaatgc gatgtgggga cggagaaggt taggttggca 1560 agctgttgga atagcttgtt taagccgggt aggtggaaga cttaggcaaa tccgggtttt 1620 cttaacaccg agaagtgatg acgagtgtct acggacacga agcaaccgat accacgcttc 1680 caggaaaagc cactaagctt cagtttgaat cgaaccgtac cgcaaaccga cacaggtggg 1740 caggatgaga attctaaggc gcttgagaga actcgggaga aggaactcgg caaattgata 1800 ccgtaacttg cgggagaagg tatgccctct aaggttaagg acttgctccg taagaccccg 1860 gagggtcgca gagaataggt ggctgcgact gtttattaaa aacacagcac tctgccaaca 1920 cgaaagtgga cgtatagggt gtgacgcctg cccggtgccg gaaggttaat tgaagatgtg 1980 caagcatcgg atcgaagccc cggtaaacgg cggtccgtaa ctataacggt acctaangta 2040 accaaattcc ttgtctnnta anttccganc ctcnctaatg gcgtaacnat gnccacactg 2100 tgncctaccg atactcaann atagttgaag tggttgttat agatgcaaaa ctatcttgct 2160 tntgnacctg nnagacaaac ntnnaccgnn nactgaagcc tttgcattgg acttggcntt 2220 angagtagcc nngaaanttc ctttttcctg gtaaaaggnn anttcctgaa ccccgccacg 2280 naattgggcg taaccgattn gcccacccct gtcctycctc cctgagactc agcgaaagtt 2340 raagtnggnt tgtgaagatg caattctacc ccgctgccta gacgggaaaa gaccccgtga 2400 accttttack gsagctttgc attgggactt tgaagtcact tgtgtaggat aggtgggagg 2460 cttggaagca gagacgccag tctnctgtgg agtcgttcct tkaaatacca cncctggtgt 2520 cntttgaagg gaatttctaa cccagacccg tcatccgggt cggggaccgt gcatggtagg 2580 cagtttgact ggggcggtct cctcccaaag cgtaacggag gagttcgaag gttacctagg 2640 tccggtcgga aatcggactg atagtgcaat ggcaaaaggt agcttaactg cgagaccgac 2700 aagtcgggca ggtgcgaaag caggacatag tgatccggtg gttctgtatg gaagggccat 2760 cgctcaacgg ataaaaggta ctccggggat aacaggctga ttccgcccaa gagttcatat 2820 cgacggcgga gtttggcacc tcgatgtcgg ctcatcacat cctggggctg tagtcggtcc 2880 caagggtatg gctgttcgcc atttaaagtg gtacgtgagc tgggtttaaa acgtcgtgag 2940 acagtttggt ccctatctgc agtgggcgtt ggaagtttga cgggggctgc tcctagtacg 3000 agaggaccgg agtggacgaa cctctggtgt accggttgta acgccagttg catagccggg 3060 tagctaagtt cggaana 3077 <210> 25 <211> 3540 <212> DNA <213> Eikenella corrodens <400> 25 cgccgtcaca ccatgggagt gggggatacc agaagcaggt agggtamccg caaggagccc 60 gcttgccncg gtatgcttca tgactggggt gaagtcgtaa caaggtagcc gtaaggggaa 120 cctgcggctg gatcacctcc tttytagaga aaaagtaggg gtagagcatc cacacctatc 180 ggtaatcaga tacagatgcg aagagaatgt tgggtttgta gctcagctgg ttagagcaca 240 cgcttgataa gcgtggggtc ggaggttcaa gtcctcccag acccaccagt agtcgtagag 300 cggagcagat ccgcccaggt actgggggca tagctcagtt ggtagagcac ctgctttgca 360 agcagggggt catcggttcg atcccgtttg cctccaccac acaatacttt ccaaataaaa 420 gtatgctaaa tatacgagta agttgctgca tcttgcagtt tgcgcggtag tttattttta 480 tttgcgaagt caaaacgcat cgatctttaa caaattggca agccgaaatc aacaaacaaa 540 cgaagtaggg cttatctttg agataagtca tacagaaatt tgggtgatga ttgtatcgac 600 tgaatcctga aacacaaaag gcaggattcg gacacaacaa gcagtaagct ttatcaaagt 660 agaggcctta agccccagct gtagtcaacg cagcgcaggc gaagtcaaaa aggcacttca 720 aatgatagag tcaagtgaat aagtgcctca ggnggatgcc ttggcnatga tnggcnacna 780 angacntgta ncccngcnaa ngctgnntnt tttnngntng ggggggntat tttttttttt 840 tttnggnaan gacgaagcgn tntttttttn nnnnntnggc ggngctgatn ccccaancna 900 agnagggggg gtnttttttt ttaagtaatc aacanaaaat ttggggattg ttnggannga 960 ttcatcccnc anaanagaaa ggannggttg gnaccaanca gnanggtttt ttttnagtag 1020 aggcgnncnn nnnccccccg tagtnanctc agcncagccg nagtnannaa nnnagntctt 1080 ttnatanant nnagtnaata antgcancan ctggangcnt nccnnatgat agnagacgaa 1140 gaangtgtnn gcatncnnnn agcatcgtgg aggtggcaan aaaannttgn tttgntnatg 1200 ncggaatgng ggggnccacc cngcaatgtg tatcntnnnc tgaatacatn agnttannta 1260 agngaacccg cngnantgat ccatcnaant anccgcngga aaagaaatca nccganattc 1320 nncnantagt ggcgagcgaa cgcggaggag cctgtatgtk atagttgtcg ggataggaga 1380 aggaattgga aacttccgcc atacgtgggt gagtagcccc gtatccgaaa ttccggcagt 1440 ggtactaagc atacgacaag tagggcggga cacgagaaat cctgtctsaa gatgggggga 1500 ccatcctcca aggctaaata ctcatcatcg atccncnnnc gatagtgaac cagtaccgtg 1560 agggaaaggc gaaaagaacc ccgggagggg agtgaaatag aacctgaaac ctgatgcata 1620 caaacagtgg gagcacttta tggtgtgact gcgtaccttt tgtataatgg gtcaacgact 1680 tacattcagt agcgagctta accggatagg ggaggcgtag ggaaaccgag tcttaatagg 1740 gcgtcatagt tgctgggtgt anacccgaaa ccgagtgatc tatccatggc cangttgaag 1800 gtgccgtaac aggtactgga ggaccgaacc cacgcatgtt gcaaaatgcg gggatgagct 1860 gtggatangg gtgaaaggct aaacaaactc ggagatagct tggttctccc cgaaaactat 1920 ttaggtngtg cctnatgtnt cacttncggg ggtaaaacac tgtnatggct anggggtcat 1980 tgnanttacc nacctatggn aaactnnnaa tacccnaaag tgcnatcatg ggaaacnnac 2040 cnnnngntgc aaacgtncnt tngncngnan ggnaacnncc cnanacnccc tctnnggncc 2100 naatgnnnna nnaaggggna aaanaaanng nagnatgcnc ntagngcatg nnanggnagn 2160 gttnngnagg cntnanaagg tgtttngtan nnaatgnngn aggnatcana agtncnaatg 2220 ttacatnagt ancnataaan cgggtnaaaa gcccgctcnc ngaaagccca aggtttcntg 2280 ngcaacgttc atcggcgcag ggtgagtcgg cccctaaggc gaggcagaga tgcgtagtcg 2340 atgggaaacg ggttaatatt cccgtacttg atttaagtgc gatgtgggga cggagaaggt 2400 taggtcagca aactgttgga atagtttgtt caagccggta ggtggaagga ttaggcaaat 2460 ccggttcttc ataacaccga gaagtgataa cgagtatcta cggatacgaa gtgactgata 2520 ccacgcttcc aggaaaagcc actaagctct agcttaaatc gaaccgtacc gcaaaccgac 2580 acaggtgggc aggatgagaa ttctaaggcg cttgagagaa ctcgggagaa ggaactcggc 2640 aaattgatac tcgtaacttn cgggnagaag gtatgccctc tgaggtgaag gatttactcc 2700 gtaagctttg gagggtcgca gcagaatcgg tggctgcgac tgtttattaa aaacacagca 2760 ctctgcaaac acgaaagtgg acgtataggg tgtgacgcct gcccggtgct ggaaggttaa 2820 ttgaagtatg tgagagcatc ggatcgaagc cccagtaaac ggcggccgta actataacgg 2880 tncctaangt agcgaanttc cttgtcngnt aagttccgan ccgcacnaat ggcgtaacga 2940 tggccacnct gtnnnctacc gngactcngc gaagttnaag tggttgtgat tcatgcaatc 3000 tactnntgnt gatntacctg aaagacccac ctgcacctgn taatgnanct ttngcnngcg 3060 naacttggct atttcgccta tntgnactga tcacgtgngg aangntcaaa aagcaccaga 3120 cgnttccttt nttgngnanc ncgtnccctg gtttggccac gntacctgtc ncnagaggna 3180 ataacccccc gtaccgcnnn nccnnngnnt cccggnanat tgnganctgg gnnngnaaac 3240 cccaaagggc nnncnngnct tgacatntcc anggncnngn gnnntnaaaa ancnttnnnt 3300 tttttttaaa nnnaaagacc tncccccctn ntttcttccn caangnntnn ctttgcaaag 3360 anncaaangn cnaaggctcn cananaaggg tntnnccgcg gggncttttc tnntccnnac 3420 nntgngggnt ggngnngggn naactnnnac cannctncnc naaaaagggn ntntttcttg 3480 ggggaaaccc gnggggtncn nnnaatnccc cnattnttcc cngnggntcc anannaacnc 3540 3540 <210> 26 <211> 2958 <212> DNA <213> Bacteroides vulgatus <400> 26 nccnnccnnn nnnaanntnn tnnnnnnnan nttcnnnnnn ggggnnnncn agtttnnnnc 60 cnnnnnnnan nnnnngggcn tnnnnnnccc ntttttttnn nnaccagccn cgtcaaagcc 120 natgggagcc ngggggtmcc tgaagntgcg taaccscgaa ggagcgnccc tasscgtaaa 180 actggtsmcc ctggggctaa gtcgtnnaam aaggtagccg tnacnnnnnn cggaaaggtg 240 cggctggaac acctcccttt cctggtangr ggacgaaaga tcgacaaggt tttttgttcc 300 tttgtactgc tggtgttgtt tattcaaaga taatgattcc gtaatgatac ggaagagaga 360 aagtaagaag ccgggtctaa tcaaacagac taggttgaac ttagtcctat agctcagttg 420 gttagagcgc tacactgata atgtagaggt cggcagttca actctgcctg ggactacgaa 480 cggaatcaag ttcgggggat tagctcagct ggctagagca tctgccttgc acgcagaggg 540 tcaacggttc gaatccgtta ttctccactc catcggaaac gtaagaaacg aagatgaacg 600 atctatgaca tattgtacaa gcaaacagta attttagtag taaagagctg aaagtatata 660 tcatncccgc tggcacgcaa gtgtgagcgn aatggnataa ggaaagtaaa raagggcgca 720 tggcggatgc cttggctctc ggaggcgatg aaggacgtga taagctgcga taagctttgg 780 gnaggtgcaa atgacctttg atccagagat ttccgaatgg gacaacccaa tatcttgaag 840 aggtattatc caacgttgtt ggaggctaac gcagggaact gaaacatctt agtacctgca 900 gcaaaagnaa ataaacaana naccccgtnt tnnnnnnnan nnangngnan canttntncn 960 ncnncantaa nngggcatgg gkgntaaggg ncngtggccn aanagnagaa gaatcnggng 1020 cacncntngn taaggtcctg naatgacagn taagttnana ntaanaaagt atgantgcta 1080 akacagctag gatgttggnt tgtagrakcm sccmttcnat ctctnagaag agntgsgtaa 1140 cagtctacac tagtmcgagg aagtcgggcg tggtataata atmgggtatt aagttgtcta 1200 cygaagcagt gggatcatta atatkatcgg taggggagca ttccagtcag cgtcgaaggm 1260 gtaccgtsag gtattmtngg agcgtctggw aaagcaaatg twggtataag taacgataaa 1320 gggggcgggn naaaccccct cgccgaaaga mtaaggtttc ctkatcaacn gctaatcgga 1380 tcagggtcag tcggstcsta aggctcagcc gaacggcgat gccgatggca raaacggtta 1440 atattccgtt actaccttca ggagtgacgt ggmgacgcag tgangntgac agctgccgcc 1500 atctracgga agntatgatg gttgaagggt gtaggagtyr atcatggcmg gcawatccac 1560 catgagcatn ccgwacctga cangtatgcc gtcstccttc gggaacaagg ctaatagcts 1620 cgcrataagc mtgctgccga gaaaanctcc gmtmaacttm mtcctgcagg ymcccstacm 1680 gcaaancgga cacacgtagt yggngngatk awtmttctaa ggcgcctntn gagtgattnc 1740 ackgttaarn gaactnaggg caaactngca ccctnktdmc ttnctgggnn rwaaarrgtc 1800 nnccymcycn ttttwtnkkk ttaggscsca gtakaatttc ggtccmgsca accttktwwt 1860 aamnnaaaaa mamaagnggc tgntgccann naatmagaaa raatccrktw tmcakcctda 1920 cmccnttgcc cgggtgctna ganngatang gttaagagra gatgtcatcg antaararaa 1980 gcgttgaatt kaagccccag taamcggcgg csgtaactat aacggtccta aggwagcgna 2040 aattncnttg tcgggtaagt tccgmcctgc mcsaatggtg taatgatctg racgctgtct 2100 caaccgtgag ctcagtgaaa ttgtagtatc ggtgaagatg ccgattaccc scratgggac 2160 gaaaagaccc cgtgamcctt tactatatcg ttaatcattg aatctgggca cgtgatgtgt 2220 aggatakgtc gaagngcntt tgaascaggt acrccagtat ttgtggagcc gctrttgaaa 2280 tacgaccctt tatkktktgc tggattytaa cgccgtngna acggrgacat tgnattggtg 2340 ccnggtastn ttgrgctggg gatggycngc ctmacaaaag cgtaacggrg ggcytcntaa 2400 aggtgtcgcc ncntccggcs gattggctwr ccggccattc cttwgaktkt aatgngcatw 2460 agcggtcgcc ttgtactsgk takwctragc aagtcnngmt ywggwaaagg aaanckrgag 2520 ncantatttt gtgcwtnccn acgccggntg ggganacgtt tnttttccrt atggttanag 2580 gangmcmttt csccntccng gtannnaann cggatcccca naaagrttaw cbccgtttcg 2640 gtcgggawaa nncaaaggnc nntgataaan nnccnctccc aararcycat atcgacggag 2700 tggtttggca cctcgatgtc ggctcgtcac atcctggggc tggagaaggt cccaagggtt 2760 gggctgttcg cccattaaag tggcacgcga gctgggttca gaacgtcgtg agacagttcg 2820 gtctctatct atcgtgggcg catgaaattt gcgtggctct gacactagta cgagaggacc 2880 gtgttggaca gacctncggt ttgccggttg tgccgccagg tgcattgccg ggtatnctaa 2940 gtncngtatc ggataaaa 2958 <210> 27 <211> 3908 <212> DNA <213> Branhamella catarrhalis <400> 27 ccccgcccgt cncaccatnt gggnagttga tctcaccaga aagtggttag cctaacgcaa 60 gagggcgatc accacggtgg ggtcgatgac tggggtgaag tcgtaacaag gtagccgtna 120 ggggaacctg cggctggatc acctccttaa cgaagttatc tgattggcaa gaatccacaa 180 caagttgttc tttggtaaga tgtttaaaaa cggggtctat agctcagttg gttagagcac 240 cgtgttgata acgcgggggt cataagttca agtcttatta gacccaccat tttggggcca 300 tagctcagtt ggtagagcgc ckgccttgca cgcaggaggt caggagttcg tactctcctt 360 ggwtccacca agcaagttta aacatwraag catacakaag caatttaaat aagatttctt 420 atttatgctt ttattttata aactgacgaa gtttataaca ttatttaaca acatagtatg 480 agtctgggtt aattatttaa ttccaacaaa taattaacct ggtgtttgta cccaatacaa 540 acaccaaaaa agtaaagaga actgaatcaa gcgtaaacat aggtgaatcg ttacacatta 600 cccatacaca ccaaagactt cctagaagtc agactacttg gggttgtatg gtcaagtaat 660 gaagtgcaca tggtggatgc cttgncactc agaggcgatg aaanacgtca taacctgcna 720 taagcntnng ngaggtggca atntccccgt gacccnncga tttcntgaat ggggaaaccc 780 aaccnacatn antttggttt attacccaan ntncttgngt aangcatnnc ccnnnntttt 840 nggngctaaa nccntngggn aaangccntn cnttncccnn aaaaggnnnn ngnttnnttt 900 ncntggnccc ccccgncnnn ttttcnnngc agncnaaaga nncccnnnaa nannnnnttt 960 tnannccnng ttttatttna aaangnngnn gattttatcn cttttttnca nnccangagt 1020 ngggntnggn tattttnttn ntttcccata annaanttcn ngtnggnttn nnccccaacc 1080 cccncccnan naaagaaaag tgnttnaagc gtnggnanan nngngncgtt ntttntcacn 1140 tacccanacc acncaantct tttnagnaag cnnacttntn ntggnatngt cgngtaatga 1200 agtgnanatc nnggnngcct ncccngncan annngatgaa anaagnnatn gcatncnatn 1260 agaancggtg nggtggcnnn ancatntnnn nngccnannt ntttttgngg gggnccancc 1320 ancatnantn ggttnttann cagcncantg tgttnggnan nccnncngga gtaanncatc 1380 ncantacccc cccnannaga catcanatna tattcngtca gtagcngcga gcgaacacgg 1440 aggagccgat yaattttwyw gtagcaanat ggcgtgggar gagccaacca tastaggtga 1500 gtagtcctgt atgcgaaact gtttaagcga catattaagt agggcggrac acgagaaatt 1560 actgttctga agtatggggg gaccatcctc ccaaggctaa atactacctg actsaccgna 1620 tagtgaacca gwaccgtgag ggaaaggcga aaagatatct tcgcgctgtt aggsgcagtg 1680 aaatagaacc tgaaaccgtg tgcatacaag acagtcggag cccgaccact taatcgtttt 1740 gaggataaca atgaattttt actacgtcta tgtcatacaa aacgtggata gtcatgatga 1800 gttttatgtt ggttttacca ccaatttaaa acaaagaatt gatgaccata ataatggcac 1860 aacttactca accagagcaa gaacatggcg tattgtttat tgtgaagcct acattaatga 1920 acaagtggca agaaaacgag agcgaaccat aaaaaataat ggtcgtatga gaactttctt 1980 gatgagatcg tgtcaaatca caatttgaca atcagaacga ttaagtggtc gggtswcggc 2040 gtaccttttg tataatgggg tcagcganct tatattctgt mncaanggtt aacncggaat 2100 wggggwgccg tanggataac cgagntttct ntaantaggk cgaatgagtt tgtcagggta 2160 tttacccnaa accgantgat ctatccatga ncnggttnga nanagtgcca tnagancagg 2220 cncctkgagg accnacccct ctgtaccctn ttannantat aagtaanccg taatagctac 2280 actagtncga gtcggcctgc gcggaaaaat gtaacggggc tcaaaaytag gagccgaagc 2340 tgcggattta attgttttca attaaagtgg taggggagcg ttgtgtaagc ctgtgaaggt 2400 gcactgtaag gtgtgctgga ggtatcacaa gagcgaatgc tgacgtgagt aacgacaaaa 2460 cgggtgaaaa gcccgttcgc cggaagacca agggttccag tccaacgtta atcggggctg 2520 ggtgagtcga cccctaaggc gaggccgaaa ggcgtastcg atgggaaatc ggttaatatt 2580 ccgatacttg tttatgatgc gatggaggga cggagaaggt tatgccagcc tggckatggt 2640 tgtccaggtg gaaggatgta gtnctatsac tgaktakgca taatccgctc ggwtattaat 2700 gakatctgwt ascamgccag tttcacctgg caaagtggya aatacsctgc ttycasgaaa 2760 agcttctamk csatagtnct ataacacgaa tcgtacccga atatccgacm caggtggyca 2820 ggtmgagamt acgnctncna aggckctnnn tgagmgtaac tctngcntag rasgnaactc 2880 rsgcwwaatg gtacccgctn acnacyycgk gagaaaskta cgctgaccga tggtkataag 2940 acnttgctst tntngagcnk nangtnnatg gcagtncnnt tgctttagat accaggcttg 3000 cnnnntgcaa ctgtttatta aawacacagc mcttcttntn gctaaacacg aaangctgsm 3060 cstwtmgggt gtgattnggn cctgcccggt gctggaaggt taattgatgg ggttagcgta 3120 agcgaagttt ytgatcgaag ccccagtaaa cggcggccgt aactataacg gtnctaaggt 3180 aannaanttn cttgtngggt aatcttccgt cctncncgan tgncatattg atggcagcgc 3240 tnnctccagn agactactcg gnganaatca aacccncnnn tnataaatgt tnngnnccct 3300 gcntnatnnn angaaacnnn ccccngngna nnctttnacn acnanntntt tnnnaaanna 3360 nnctncncac cctnacactt ctnatacnnn nntcgngnnt cggaannctt ttcnnaatnn 3420 nanttacttc ccactcgtac ggntntntna tncccncacn ctaanncntn gcccacgcnn 3480 ttgtgnngnt nntnntggta ctttctnacn cantnagacn anccanncca angtccnnna 3540 nggancccct cntnnnngnn cnaanttnac nntttnngat nnccnacann naancncccc 3600 ngtnnnaaaa nttttngacn nnaatgagct actcgtaacn cgctanctac ngncctcanc 3660 nntnantcnn natnaagtgt ggtnncacnn nnctnnaaat nntaattncn cntnntntnc 3720 nnntntttct ctctnntacc ttcgnnncnn natananntt tnngctcntc tcnaattacn 3780 gtgcntntta catacnnnat ntacaatntc nngcncgctn ctngtnttac tnngnnttnc 3840 cnctnnncna ctnancattc ntatacnngt tttgnncctt ntccntnact ntacctacgn 3900 nnttcnnt 3908 <210> 28 <211> 3166 <212> DNA <213> Sutterella wadsworthia <220> <221> rRNA (222) (1) .. (3166) <223> 23S rRNA and Internal Transcribed Spacer Region <400> 28 ggcgcccacc ggggttcagg ctgggggtca actttaacaa ggtagccctc cggaaggtcc 60 ggctggatca cctcctttaa acagagtcgg ccttgaagcc gccgcaccta cggagtgggc 120 ttcaccagga atcgttggcc ctcccgaaca aggagggggg ttctaggtgg gcttcttaaa 180 ggatggtaag tagaagcaag ggggccagta cttcaagtcc cgccagaatc acctcttttc 240 aagaaagtgg gccttatgca gaatcgacat caagaactgt tgggggcgtt cacacttatc 300 gagttctgtt gaattcaatt cgcccgattc tttaaaaatt tggaaaagta aagcaacaga 360 agctgctgct ttcgaagggt tgagatgctc tttgaaggcg caaaaaggca gcttcagggg 420 ttgtgattgc attcacgaac ccgattcatt gaaagttctt caaagaaccg acatgattca 480 agagaaatac agcgttatag gtcgtcttgt gctttgaagg caaaaggctt cagagttata 540 ggatcaagcg actaagtgta tgtggtggat gccttggcaa aatctacatg gtcgaagaag 600 gtacgtcgtc atcctgtcga aaagccgtgg ggagcctggc aagcgagcca ctgatgccac 660 ggatgtccga atgagggaac tcacccgcaa gggtatcttg aagtgaatac atagcttcaa 720 gaggcgaacg cagcgaactg aaacatctaa gtagctgcag gaaaagaaat caaccgagat 780 tctgaaagta gcggcgagcg aaattggaag agcctgcaag tgatattgaa ttcgatactg 840 gaacggtctg gaaagtccgg ctacagaggg tgacagcccc gtacgggaaa ttgggttcaa 900 ggtactgagc ttgcgagaag tagagcggga cacgtgaatc atccttgctc gaagatgggg 960 ggaccatcct ccaaggctaa atactcgtaa tcgaccgata gcgaacccag tactgtgaag 1020 gaaaggcgaa aagaaccccg ggaggggagt gaaatagatc ctgaaaccgc atacatacaa 1080 acagtaggag cccgcaaggg tgactgcgta ccttttgcat aatgggtcag cgacttacgt 1140 tcaatggcaa gcttaaccga gtaggggagg cgcagcgaaa gcgagtccga acagggcgat 1200 atgagtcgtt gggcgtagac ccgaaaccag atgatctatc catggccagg ctgaaggtgt 1260 ggtaacacac actggagggc cgaaccgact agtgttgcaa aattagcgga tgagctgtgg 1320 ataggggtga aaggctaaac aaatctggag atagctggtt ctccccgaaa actattgagg 1380 tagtgcctcg tgtatggctc cagggggtag agcactgtta tggctagggg gacatggcgt 1440 cttaccaaac catggcaaac tccgaatact tggaagtctg agcacgggag acagagcacc 1500 gggtgctaac gtccgggact caaaagggaa acaacccaga ccgccggcta aggtccctaa 1560 tattgactaa gtggaaaaac gaagtgggaa ggctaaaaca gtcaggaagt tggcttaaac 1620 caccccaatt cactttcaaa gaaaccgtaa tagctaactg atcgagtctt cctgcgccgg 1680 aaaaatgtaa cggggctaag tcaataaccg aagccgcgga ttcgcagcaa tgcagtggta 1740 ggggaagcgt tctgtaagcc tgcaaggcgg atccgcgagg accgctggag gtatcagaag 1800 tgcgaatgct gacatgagta acgttaaagc gggtgaaaag cccctcgcca gcccaagggg 1860 ggacggagtc ctgaaggtca tccgggtgtt ggacatcccg gtttacgagc gtaggaggtt 1920 gacaggcaaa tccgtcaacg gatctccaag gcgaaggatc gaggttgaga gatcaactga 1980 agtgactgaa tgggcttcca ggaaaagcct ctaagcatca gcttggaatt gaccgtaccg 2040 caaaccgaca ctggtgggcg agctgaatat gatcaggcgc ttgagagaac acaggagaag 2100 gaactcggca aattgacacc gtaacttcgg gataaggtgt gcctttgtag tgtgaagaga 2160 gaaacactcc gagcatgaag aggcctcaga gaatcggtgg ctgcaactgt ttactaaaaa 2220 cacagcactc ctgcaaagat gaaagtacga cgtatagggt gtgatgcctg cccggtgctg 2280 gaagattaat tgatggggtg caagctcctg atcgaagtcc cagtaaacgg cggccgtaac 2340 tataacggtc cctaaaggag cgaaattcct tgtcgggcaa gttccgacct gcagaatggc 2400 aaatgatggc cacactgtct cctcctagac tcagtaagtt gaaatgtttg tgatgatgca 2460 atctcccccg gctagacgga aagaccccat aaccttactg cagctttgca ttggactttg 2520 ataccgatct gtgtaggata ggtgggaggc agtgaagcta ggacgccagt cttagcggag 2580 ccaaccttca aataccaccc tgatttgttt gaggttacta acctaggcag acgtaagccg 2640 attgcggggc accgtgcatc ctaggcagtt tgatgggcgg tctcctccta aagggtaacg 2700 gaggagtgcg aaggtacgct aggtacggtc ggaaatcgtg ctgatagtgc aatggcaaaa 2760 gcgtgcttga ctgcgagacc gacaagtcga gcagatacga aagtaggtca tagtgatccg 2820 gtggctctgc atggaagggc catcgctcaa cggataaaag gtactctggg gataacaggc 2880 tgataccgcc caagagttca tatcgacggc ggtgtttggc acctcgatgt cggctcatct 2940 catcctgggg ctgtagccgg tcccaagggt atggctgttc gccatttaaa gaggtacgtg 3000 agctgggttt aaaacgtcgt gagacagttt tgtccctatc tgccgtgggc gttggaagat 3060 tgacgggggc tgctcctagt acgagaggac cggagtggac tcacctctgg tgtaccggtt 3120 gtcacgccag ggcatcgccg ggtaggctat gtgggaaagg aaaaca 3166 <210> 29 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acti004 <400> 29 tgatggaact tgctt 15 <210> 30 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acti23S01 <400> 30 agggcacaca taatg 15 <210> 31 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acti23S02 <400> 31 acgctgttgt tggtg 15 <210> 32 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acti3 (Acti I) <400> 32 atacacagta cttcg 15 <210> 33 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acti002 <400> 33 atagtgttgc aaggc 15 <210> 34 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acti003 <400> 34 tgaaaagcca gggga 15 <210> 35 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acti1 (Acti 23) <400> 35 gggcacacat aatga 15 <210> 36 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acti2 (Acti M) <400> 36 cggggtactc tatac 15 <210> 37 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acti23S03 <400> 37 gtaggtatgt atctt 15 <210> 38 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acti23S04 <400> 38 tactgagatc cgata 15 <210> 39 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acti001 <400> 39 aggtattgca acatg 15 <210> 40 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe ActiIT01 <400> 40 cagaagtagc tgcct 15 <210> 41 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe ActiIT02 <400> 41 agaagtagct gccta 15 <210> 42 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe ActiIT03 <400> 42 gaagtagctg cctaa 15 <210> 43 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe ActiIT04 <400> 43 aagtagctgc ctaac 15 <210> 44 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe ActiIT05 <400> 44 agtagctgcc taact 15 <210> 45 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas001 <400> 45 tgactcgtgc ccatg 15 <210> 46 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas002 <400> 46 taccggggtt aaaag 15 <210> 47 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas003 <400> 47 atcagtgatc tgaga 15 <210> 48 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas004 <400> 48 gagacgaagc accat 15 <210> 49 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas011 <400> 49 agttgataca ggtag 15 <210> 50 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas013 <400> 50 ggccccatcc ggggt 15 <210> 51 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas23S03 <400> 51 cagttggaag cagag 15 <210> 52 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas005 <400> 52 gttcttgatt cattg 15 <210> 53 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas008 <400> 53 cagcccaaaa gttga 15 <210> 54 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas009 <400> 54 aaactgcagg gcaca 15 <210> 55 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas010 <400> 55 atactacctg acgac 15 <210> 56 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas 1 (Anas 7) <400> 56 aaagtgcagg gcaca 15 <210> 57 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas 2 (AnasM) <400> 57 tggattgtgg tgaaa 15 <210> 58 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas 3 (Anas 7) <400> 58 tagcgttctg cgagg 15 <210> 59 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas 4 (AnasM) <400> 59 ttaaaagact ggtat 15 <210> 60 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas006 <400> 60 atccaatcat gatca 15 <210> 61 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas007 <400> 61 aagcatgaaa gcgca 15 <210> 62 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas014 <400> 62 gaggcgggag ccgag 15 <210> 63 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas23001 <400> 63 ccccatccgg ggttg 15 <210> 64 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas23S01 <400> 64 tggcgtcagg aggcg 15 <210> 65 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas23S02 <400> 65 ataaggggcg cttga 15 <210> 66 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas23S04 <400> 66 tcacacgcaa gtgtg 15 <210> 67 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas23S05 <400> 67 gctgagacga agcac 15 <210> 68 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas23S06 <400> 68 ataccggggt taaaa 15 <210> 69 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas23S03 <400> 69 cagttggaag cagag 15 <210> 70 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Anas012 <400> 70 tagcgttctg cgagg 15 <210> 71 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe AnasIT001 <400> 71 acagcgcagc atgtg 15 <210> 72 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe AnasIT002 <400> 72 aattagcaac tattt 15 <210> 73 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe AnasIT01 <400> 73 cttccctcag tgatt 15 <210> 74 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe AnasIT02 <400> 74 ttccctcagt gattc 15 <210> 75 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe AnasIT03 <400> 75 tccctcagtg attca 15 <210> 76 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe AnasIT04 <400> 76 ccctcagtga ttcaa 15 <210> 77 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe AnasIT05 <400> 77 cctcagtgat tcaag 15 <210> 78 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bf 011 <400> 78 gtcgaacctg acagt 15 <210> 79 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bacf1 (Bf23) <400> 79 ggtaaccgaa gcgta 15 <210> 80 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bacf2 (Bf) <400> 80 ctcggaaaac ggtaa 15 <210> 81 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bf 001 <400> 81 agcgatgttg aaaac 15 <210> 82 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bf 002 <400> 82 tcaaccatct atagc 15 <210> 83 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bf 003 <400> 83 aacaagagaa aaaca 15 <210> 84 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bf 004 <400> 84 cgataccgcg accta 15 <210> 85 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bf 005 <400> 85 tatatcgaac cattt 15 <210> 86 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bf 006 <400> 86 gaatctggcg ataaa 15 <210> 87 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bf 007 <400> 87 tgcaaatgac ctttg 15 <210> 88 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bf 008 <400> 88 caacttggtt ggagg 15 <210> 89 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bf 009 <400> 89 acccatgtta cggca 15 <210> 90 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bf 010 <400> 90 agttgaccta acgaa 15 <210> 91 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bf 012 <400> 91 tgaacggatc tgtgt 15 <210> 92 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bacf3 (Bf I) <400> 92 ggttcagatc ctttt 15 <210> 93 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Car 006 <400> 93 aaccctggtg aaggg 15 <210> 94 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Car 007 <400> 94 atatgaagat atgtg 15 <210> 95 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Car 008 <400> 95 tagattgact tacgg 15 <210> 96 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Car 009 <400> 96 gtaaagtttt actac 15 <210> 97 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Car2 (CarM) <400> 97 ccagcacact gttgg 15 <210> 98 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Car 3 (CarI) <400> 98 aaagagagaa cagca 15 <210> 99 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Car 001 <400> 99 ttggcgacaa caggc 15 <210> 100 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Car 002 <400> 100 gccccgggaa gctga 15 <210> 101 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Car 003 <400> 101 tagactgcgg aagcg 15 <210> 102 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Car 004 <400> 102 aattaagttg cgtat 15 <210> 103 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Car1 (Car23) <400> 103 ccatacacaa tgaat 15 <210> 104 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Car 005 <400> 104 tactcgttgt cgacc 15 <210> 105 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Chr23S04 <400> 105 ggcatattta gatga 15 <210> 106 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Chr001 <400> 106 cttaggtgat cactt 15 <210> 107 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Chr003 <400> 107 taacccctta gatta 15 <210> 108 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Chr004 <400> 108 tcaaacctca aacta 15 <210> 109 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Chr005 <400> 109 aagaaatcga agaga 15 <210> 110 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Chry1 (Chry23) <400> 110 atttagatga taaat 15 <210> 111 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Chry2 (Chry7) <400> 111 taatcttact agcga 15 <210> 112 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Chr23S05 <400> 112 atcgtgaggt tacga 15 <210> 113 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Chry3 (ChryI) <400> 113 tccttgagtg cagag 15 <210> 114 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Chr002 <400> 114 agcacagctt tggtt 15 <210> 115 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.ramosa04 <400> 115 ccagtgtgtg aggag 15 <210> 116 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.ramo 004 <400> 116 cccgggaagg ggagt 15 <210> 117 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.ramosa01 <400> 117 ggtgaagtat tagta 15 <210> 118 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.ramosa02 <400> 118 atgtacaggc atagg 15 <210> 119 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.ramosa03 <400> 119 tgagagacat gcacg 15 <210> 120 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.ramosa05 <400> 120 gtattggagt tgcta 15 <210> 121 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.ramo 001 <400> 121 tagttgatga tagta 15 <210> 122 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.ramo 002 <400> 122 gcttatctgt ggatg 15 <210> 123 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.ramo 003 <400> 123 ggaatccctc cttgt 15 <210> 124 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Com 004 <400> 124 tagggcgtcc agtcg 15 <210> 125 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Com 005 <400> 125 cgcagagtac agctt 15 <210> 126 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Com 006 <400> 126 gtaccgatgt gtagt 15 <210> 127 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Com 007 <400> 127 gaacttgaac aaagg 15 <210> 128 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Coma2 (Com 7) <400> 128 tgagctagag aaaag 15 <210> 129 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Coma 3 (Com M) <400> 129 atccgccggg cttag 15 <210> 130 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Coma1 (Com23) <400> 130 aaaaccgact ggtgg 15 <210> 131 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Com 001 <400> 131 gctgacggaa agaga 15 <210> 132 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Com 002 <400> 132 ctcttgacag aaatg 15 <210> 133 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Com 003 <133> 133 aagaattcat tcaca 15 <210> 134 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Coma 4 (Com I) <400> 134 acgcgcgagg tgaga 15 <210> 135 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.diph003 <400> 135 accatcttcc caagg 15 <210> 136 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.dipht 01 <400> 136 taactagata agaaa 15 <210> 137 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.diph001 <400> 137 accacgcagc agttt 15 <210> 138 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.diph002 <400> 138 cgagtcggta gggta 15 <139> <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.diph004 <400> 139 tgtttgttct ttgat 15 <210> 140 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.diph005 <400> 140 aaaatcagaa aaaca 15 <210> 141 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.diph006 <400> 141 ggaaaatcag aaaaa 15 <210> 142 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ko001 <400> 142 gaacgttact aacgc 15 <210> 143 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Koxy1 (Ko M) <400> 143 ccggaacgtt actaa 15 <210> 144 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Koxy3 (Ko M) <400> 144 aagagcgcca gctca 15 <210> 145 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ko001 <400> 145 tttgaagttc taact 15 <210> 146 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ko002 <400> 146 aagagcgcca gctac 15 <210> 147 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ko002 <400> 147 tatctaccgc gggcg 15 <210> 148 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ko003 <400> 148 gatgaagacc tcaaa 15 <210> 149 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ko003 <400> 149 ttacgggttg tcatg 15 <210> 150 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Koxy2 (Ko I) <400> 150 cgcgacacga cgatg 15 <210> 151 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ochr04 <400> 151 ggaccaggcc agtgg 15 <210> 152 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ochr05 <400> 152 gaccaggcca gtggc 15 <210> 153 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ochr004 <400> 153 gttgattgac acttg 15 <210> 154 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ochr005 <400> 154 taccgctcac gagcc 15 <210> 155 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ochr007 <400> 155 gggtccggag gttca 15 <210> 156 <211> 15 <212> DNA <213> Artificial Sequence <220> Probe Ochr 1 (Ochr 23-1) <400> 156 cggcgcgtga gcgag 15 <210> 157 <211> 15 <212> DNA <213> Artificial Sequence <220> Probe Ochr2 (Ochr 7-1) <400> 157 gaacacctgt tgtcc 15 <210> 158 <211> 15 <212> DNA <213> Artificial Sequence <220> Probe Ochr 4 (Ochr 23-2) <400> 158 tcgtcggccc atgtg 15 <210> 159 <211> 15 <212> DNA <213> Artificial Sequence <220> Probe Ochr 5 (Ochr 7-2) <400> 159 ttagtgtatc gagca 15 <210> 160 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ochr002 <400> 160 cttcgggctg atgat 15 <210> 161 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ochr003 <400> 161 aggccagtca gcctg 15 <210> 162 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ochr006 <400> 162 gttggttctg ataca 15 <210> 163 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ochr008 <400> 163 cagttggaag cagag 15 <210> 164 <211> 15 <212> DNA <213> Artificial Sequence <220> Probe Ochr 3 (Ochr I) <400> 164 gatccgacga tttcc 15 <210> 165 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ochr001 <400> 165 taggaaagac gcagt 15 <210> 166 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pep002 <400> 166 actagggaga gctca 15 <210> 167 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pep003 <400> 167 gcttagtaaa gcaag 15 <210> 168 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pep004 <400> 168 tactaacatg tgacc 15 <210> 169 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pep005 <400> 169 aagcagagag agctc 15 <210> 170 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pep006 <400> 170 cgaacggtga ggccg 15 <210> 171 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pep007 <400> 171 gtagatgttg attat 15 <210> 172 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pep23S02 <400> 172 gtcgaatcat ctggg 15 <210> 173 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pep23S03 <400> 173 taaaacgtat cggat 15 <210> 174 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pep1 (Pep23) <400> 174 actaaataaa ccagg 15 <175> 175 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pep2 (PepM) <400> 175 ataatcaaca tctac 15 <210> 176 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pep3 (PepI) <400> 176 tctgtataat agttc 15 <210> 177 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pep001 <400> 177 agaagctgat acgtc 15 <210> 178 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Por 003 <400> 178 agttggtgag cgagc 15 <210> 179 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Por23S08 <400> 179 ctgagctgtc gtgca 15 <210> 180 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Por 001 <400> 180 gtttttgtga gtgga 15 <210> 181 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Por 002 <400> 181 tgatgggtgg ggttg 15 <210> 182 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Por1 (Por7) <400> 182 gttggatgtt atcat 15 <210> 183 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Por2 (PorM) <400> 183 cgggcagcta aaacc 15 <210> 184 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Por 004 <400> 184 acctatgagt actat 15 <210> 185 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Por 3 (PorI) <400> 185 tgtttgtgcg acgtg 15 <210> 186 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.anae003 <400> 186 aggaggaaga gaaag 15 <210> 187 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.anae004 <400> 187 gcgaaaggaa aagag 15 <210> 188 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.anae001 <400> 188 tgcattacta agtga 15 <210> 189 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.anae002 <400> 189 gtaaggtcga taccc 15 <210> 190 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.magn002 <400> 190 catgcaacga tccgt 15 <210> 191 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.magn001 <400> 191 tagttgaaaa tagta 15 <210> 192 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.magn003 <400> 192 cagcacgtga atatg 15 <210> 193 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe f.necro01 <400> 193 tttcgcagac gtaag 15 <210> 194 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe f.necro02 <400> 194 gttttcttgc gctgt 15 <210> 195 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe f.necro03 <400> 195 ccgtattcat gtcaa 15 <210> 196 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe f.necro05 <400> 196 ctgcaagcta tttcg 15 <210> 197 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe f.necro06 <400> 197 cagacgtaag caaag 15 <210> 198 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe f.necro07 <400> 198 cctgtattgg tagtt 15 <210> 199 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.vulga004 <400> 199 agaggaggct tagtg 15 <210> 200 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.vulga01 <400> 200 atacgtgtta tgtgc 15 <210> 201 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.vulga03 <400> 201 atatccaatg gatat 15 <210> 202 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.vulga006 <400> 202 ggaaacccaa tatcc 15 <210> 203 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.vulga007 <400> 203 gggaaaccca atatc 15 <210> 204 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.vulga008 <400> 204 cactgtttcg actag 15 <210> 205 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.vulga02 <400> 205 ctcacacaga cttgt 15 <206> 206 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.vulga005 <400> 206 gtgggttgca aaata 15 <210> 207 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.aero01 <400> 207 ttccgacggt acagg 15 <210> 208 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.aero03 <400> 208 gtatcagtaa gtgcg 15 <210> 209 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.aero04 <400> 209 ttatccaggc aaatc 15 <210> 210 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.aero02 <400> 210 gagcggggta gttga 15 <210> 211 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.aero005 <400> 211 aatcaaggct gaggt 15 <210> 212 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S.mutan001 <400> 212 taggtattct ctcct 15 <210> 213 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S.mutans01 <400> 213 gaaaaacgaa gggta 15 <210> 214 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S.mutans02 <400> 214 atgactacgt ggtcg 15 <210> 215 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S.mutans03 <400> 215 gtaatgcaag atatc 15 <210> 216 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S.mutans004 <400> 216 ttgtatgcgc ggtag 15 <210> 217 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S.mutans005 <400> 217 cgaaaagtat cgggg 15 <210> 218 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe K.king02 <400> 218 ggttagcaaa ctgtt 15 <210> 219 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe K.king03 <400> 219 ccagtaggtg gaaag 15 <210> 220 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe K.king04 <400> 220 aacaccgaga cgtga 15 <210> 221 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe K.king09 <400> 221 tattcaatgc gatgg 15 <210> 222 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe K.king01 <400> 222 tgattcaatg cgatg 15 <210> 223 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe K.king05 <400> 223 tataattaaa cgcat 15 <210> 224 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe K.king06 <400> 224 aatgttgtcg atttg 15 <210> 225 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe K.king07 <400> 225 aggcaacaaa tcgaa 15 <210> 226 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe K.king08 <400> 226 tatcaactaa tcttg 15 <210> 227 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.ovatus01 <400> 227 tagaaggaag cattc 15 <210> 228 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.ovatus02 <400> 228 ccaatgttgt tacgg 15 <210> 229 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.ovatus005 <400> 229 tgtaggacca cgatg 15 <210> 230 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.ovatus003 <400> 230 ggaccgaacc gataa 15 <210> 231 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.ovatus004 <400> 231 ggacacgagg aatct 15 <210> 232 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.ovatus006 <400> 232 tgaaggaatg tcatc 15 <210> 233 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.ovatus007 <400> 233 cccacgatag ataga 15 <210> 234 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.thetaio006 <400> 234 gctaacgcag ggaac 15 <210> 235 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.thetaio01 <400> 235 ttattgtact actgg 15 <210> 236 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.thetaio02 <400> 236 atcaggtaga caagg 15 <210> 237 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.thetaio03 <400> 237 ttgtcgttgc caata 15 <210> 238 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.thetaio04 <400> 238 cagtgttgga atgtt 15 <210> 239 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.thetaio05 <400> 239 actatactat agtca 15 <210> 240 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.diffc005 <400> 240 gttcgtccgc ccctg 15 <210> 241 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.diffc001 <400> 241 gcatatatat ttagt 15 <210> 242 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.diffc002 <400> 242 gatatgacat ctaat 15 <210> 243 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.diffc003 <400> 243 tttcggggag ttgca 15 <210> 244 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.diffc004 <400> 244 catgtggaca gtatg 15 <210> 245 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe H.aphro003 <400> 245 ggtgaagaac ccact 15 <210> 246 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe H.aphro001 <400> 246 tgggagtggg ttgtc 15 <210> 247 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe H.aphro002 <400> 247 taacaaaccg gaaac 15 <210> 248 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe H.aphro004 <400> 248 atcattatct gaatc 15 <210> 249 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe H.aphro005 <400> 249 agaaatcaac cgtag 15 <210> 250 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe H.aphro006 <400> 250 attagcggat gactc 15 <210> 251 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe H.aphro007 <400> 251 aacccagtgg gtgaa 15 <210> 252 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe H.aphro008 <400> 252 aaacccagtg ggtga 15 <210> 253 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe H.aphro009 <400> 253 gaaacccagt gggtg 15 <210> 254 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe N.gono005 <400> 254 tatcaaagta gggat 15 <210> 255 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe N.gono006 <400> 255 agtcaacggg taggt 15 <210> 256 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe N.gono002 <400> 256 aacctctcgc aagag 15 <210> 257 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe N.gono003 <400> 257 catagtattt gggtg 15 <210> 258 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe N.gono004 <400> 258 ttgtatcaga cttaa 15 <210> 259 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe N.gono001 <400> 259 aatgagtttg ttttg 15 <210> 260 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe N.gono007 <400> 260 caatgagttt gtttt 15 <210> 261 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe N.gono008 <400> 261 cgtaactata acggt 15 <210> 262 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.corro005 <400> 262 ggataggaga aggaa 15 <210> 263 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.corro006 <400> 263 actcatcatc gatcc 15 <210> 264 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.corro001 <400> 264 agtcgtagag cggag 15 <210> 265 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.corro002 <400> 265 agatccgccc aggta 15 <210> 266 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.corro003 <400> 266 gttgctgcat cttgc 15 <210> 267 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.corro004 <400> 267 gcaggattcg gacac 15 <210> 268 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.vulga03 <400> 268 agtcagcgtc gaagg 15 <210> 269 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.vulga07 <400> 269 cgaatgcgca tcagt 15 <210> 270 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.vulga01 <400> 270 catcttgaga tgtgc 15 <210> 271 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.vulga02 <400> 271 agtcgggcgt ggata 15 <210> 272 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.vulga04 <400> 272 acgctaatcg gatca 15 <210> 273 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.vulga05 <400> 273 gaccgataga gcatg 15 <210> 274 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.vulga06 <400> 274 tgacacactg taact 15 <210> 275 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.vulga08 <400> 275 attgtcatga gccac 15 <210> 276 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.vulga09 <400> 276 aatttgcgtg gctct 15 <210> 277 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.vulga10 <400> 277 ctccatcgga aacgt 15 <210> 278 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.vulga11 <400> 278 actccatcgg aaacg 15 <210> 279 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.vulga12 <400> 279 gggactacga acgga 15 <210> 280 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.catar005 <400> 280 atatcttcgc gctgt 15 <210> 281 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.catar001 <400> 281 agtctgggtt aatta 15 <210> 282 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.catar002 <400> 282 acaagttgtt ctttg 15 <210> 283 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.catar003 <400> 283 aacataggtg aatcg 15 <210> 284 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.catar004 <400> 284 aagtaatgaa gtgca 15 <210> 285 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.catar006 <400> 285 gaggataaca atgaa 15 <210> 286 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.catar007 <400> 286 cgaatgagtt tgtca 15 <210> 287 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.catar008 <400> 287 acccgaatat ccgac 15 <210> 288 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.catar009 <400> 288 gacccaccat tttgg 15 <210> 289 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.catar010 <400> 289 ataatggggt cagcg 15 <210> 290 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.catar011 <400> 290 agcctgtgaa ggtgc 15 <210> 291 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe B.catar012 <400> 291 aagaattgat gacca 15 <210> 292 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S.wad 02 <400> 292 gctccgacaa gaact 15 <210> 293 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S.wad03 <400> 293 cgagttgttg aattc 15 <210> 294 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S.wad04 <400> 294 gtcgtcttgt gcttt 15 <210> 295 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S.wad 01 <400> 295 ctcagtaaga cgttt 15 <210> 296 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acii1 (Acii23) <400> 296 aacctggctg gtggc 15 <210> 297 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acii2 (AciiM) <400> 297 gacacttttg tgtca 15 <210> 298 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Acii3 (Aci I) <400> 298 gttgggtggt tgcct 15 <210> 299 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe SeM01 <400> 299 gatagataac aggtg 15 <210> 300 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe SeM02 <400> 300 agggttcacg cccag 15 <210> 301 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Se1 (Se 23) <400> 301 ttctctcttg agtgg 15 <210> 302 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Se2 (Se M) <400> 302 cgtgctgttg gagtg 15 <210> 303 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Se3 (Se I) <400> 303 gctatttatt ttgaa 15 <210> 304 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bur (Bur23) <400> 304 ttgttagccg aacgc 15 <210> 305 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bur01 <400> 305 gggtgtggcg cgagc 15 <210> 306 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Bur001 <400> 306 gccaggaggg tgaag 15 <210> 307 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Styp (Styp23) <400> 307 gcctgaatca gcatg 15 <210> 308 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Styp01 <400> 308 gctgaggata cggtt 15 <210> 309 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Styp02 <400> 309 ccgcaaaaca agcag 15 <210> 310 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Styp03 <400> 310 acgattgacg gagcg 15 <210> 311 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Sal.typ001 <400> 311 tcgcgccgtc acagt 15 <210> 312 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E coli 003 <400> 312 ctgaagcgac aaatg 15 <210> 313 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Eco1 (Eco23) <400> 313 gagcctgaat cagtg 15 <210> 314 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Eco2 (Ecoli) <400> 314 gttagcggta acgcg 15 <210> 315 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E coli 001 <400> 315 gttagcggta acgcg 15 <210> 316 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E coli 002 <400> 316 atgcacatat tgtga 15 <210> 317 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Kpneu (Kpneu 23) <400> 317 gtacaccaaa atgca 15 <210> 318 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe K.pneu002 <400> 318 gctgagacca gtcga 15 <210> 319 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe K.pneu001 <400> 319 acgctggtgt gtagg 15 <210> 320 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Kpneu01 <400> 320 accttcgggt gtgac 15 <210> 321 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pm <400> 321 gttaccaaca atcgt 15 <210> 322 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pm002 <400> 322 ggcgacggtc gtccc 15 <210> 323 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pm003 <400> 323 gatgacgaac cacca 15 <210> 324 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pm004 <400> 324 tgaagcaatt gatgc 15 <210> 325 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pm001 <400> 325 aaggctaggt tgtcc 15 <210> 326 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pm005 <400> 326 taaagtccct cgcgg 15 <210> 327 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pm01 <400> 327 aggcagagtg attag 15 <210> 328 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Strepp (StreppM) <400> 328 taggactgca atgtg 15 <210> 329 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Strepp01 <400> 329 atgtggtaca gacac 15 <210> 330 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Strepp02 <400> 330 ggttaaacgc tagaa 15 <210> 331 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Strepp03 <400> 331 caggatactg ctaag 15 <210> 332 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Strepp04 <400> 332 gagtaaactc ttcgg 15 <210> 333 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Vvul02 <400> 333 gttgacgatg catgt 15 <210> 334 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe VvulM <400> 334 agtaacagcc acttg 15 <210> 335 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe V.vul001 <400> 335 atagctcaat gaagc 15 <210> 336 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe V.vul002 <400> 336 ggcgccatag tctct 15 <210> 337 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Vvul 01 <400> 337 tttacatgtg ttaga 15 <210> 338 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Vvul 03 <400> 338 gttctatgaa cattg 15 <210> 339 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.aeru001 <400> 339 gaagtgccga gcatg 15 <210> 340 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pa03 <400> 340 ggatctttga agtga 15 <210> 341 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pa <400> 341 gtttcccgtg aaggc 15 <210> 342 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.aeru002 <400> 342 gtgtcacgta agtga 15 <210> 343 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.aeru003 <400> 343 agtcgtcttt tagat 15 <210> 344 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe P.aeru004 <400> 344 actccgtaag ctctg 15 <210> 345 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pa01 <400> 345 taggataacc taggt 15 <210> 346 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Pa02 <400> 346 taagcttcat tgatt 15 <210> 347 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ah <400> 347 ggcgcctcgg taggg 15 <210> 348 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ae.hy 001 <400> 348 taagccgtga gcagt 15 <210> 349 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ae.hy 002 <400> 349 catcttggaa gttag 15 <210> 350 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ae.hy 003 <400> 350 tcaaaccagg caccg 15 <210> 351 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ae.hy 004 <400> 351 gattcacgct aagcg 15 <352> 352 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ae.hy 005 <400> 352 acggtgcgga agcca 15 <210> 353 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Ah01 <400> 353 cacgaaaaca acctt 15 <210> 354 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe LM <400> 354 gggtgcaagc ccgag 15 <210> 355 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Lm01 <400> 355 agtatcctta cgtga 15 <210> 356 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Lm02 <400> 356 gtgaggaagg cagac 15 <210> 357 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Lm03 <400> 357 ggctttccct ccaga 15 <210> 358 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Lm04 <400> 358 ccgcttctca cgaag 15 <210> 359 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.faecium002 <400> 359 ttacgattgt gtgaa 15 <210> 360 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.faecium003 <400> 360 atagcacatt cgagg 15 <210> 361 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Enfcium1 (Enfaeci23) <400> 361 gttctttcag atagt 15 <210> 362 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Enfcium2 (Enfaeci M) <400> 362 ctgaagagga gtcaa 15 <210> 363 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.faecium001 <400> 363 gctgatcata cgatc 15 <210> 364 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe E.faecium004 <400> 364 cttcttttct taagg 15 <210> 365 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S. aureus 004 <400> 365 gattgcacgt ctaag 15 <210> 366 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S. aureus 005 <400> 366 aatccggtac tcgtt 15 <210> 367 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Saure03 <400> 367 tcttcgagtc gttga 15 <210> 368 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Saur <400> 368 gttaacgccc agaag 15 <210> 369 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S. aureus 001 <400> 369 aggacgacat tagac 15 <210> 370 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S. aureus 002 <400> 370 aaaatgttgt ctctc 15 <210> 371 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe S. aureus003 <400> 371 cgaagcgtgc gattg 15 <210> 372 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Saure01 <400> 372 aagcagtaaa tgtgg 15 <210> 373 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Saure02 <400> 373 gagaagacat tgtgt 15 <210> 374 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Saureus01 <400> 374 atatcagaag gcaca 15 <210> 375 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Saureus02 <400> 375 acaaaggacg acatt 15 <210> 376 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Saureus03 <400> 376 tcttcgagtc gttga 15 <210> 377 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Nm002 <400> 377 agatgtgaga gcatc 15 <210> 378 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Nm1 (Nm) <400> 378 ccctggaggg tcgca 15 <210> 379 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Nm2 (Nm-1) <400> 379 tttgaattga accgt 15 <210> 380 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Nm001 <400> 380 gtttactggc atggt 15 <210> 381 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Nm01 <400> 381 taaagcaatg atccc 15 <210> 382 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe Nm02 <400> 382 ccgggtcttc ttaac 15 <210> 383 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu011 <400> 383 tggagagcat tttat 15 <210> 384 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu012 <400> 384 gtgattttga ggtga 15 <210> 385 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu013 <400> 385 agatggtaaa gaaga 15 <210> 386 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu001 <400> 386 cctcaagatg agttt 15 <210> 387 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu002 <400> 387 gaagcccgtt gaaga 15 <210> 388 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu003 <400> 388 gcagtaatgc gtgaa 15 <210> 389 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu004 <400> 389 ttgtcttgac catat 15 <210> 390 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu005 <400> 390 accatataat ctgag 15 <210> 391 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu006 <400> 391 tgcccacaca gtttg 15 <210> 392 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu007 <400> 392 caaagtgccc acaca 15 <210> 393 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu008 <400> 393 tgattttgag gtgat 15 <210> 394 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu009 <400> 394 ccaccattta atgat 15 <210> 395 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe L.pneu010 <400> 395 agcattttat tctgg 15 <210> 396 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.alic001 <400> 396 tggtagccat ttatg 15 <210> 397 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.alic003 <400> 397 ctggaccagc cgagc 15 <210> 398 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.alic006 <400> 398 tcaagaacga aagtt 15 <210> 399 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.alic007 <400> 399 aaggattgac agatt 15 <210> 400 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.alic008 <400> 400 cattaatcaa gaacg 15 <210> 401 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.alic002 <400> 401 ttttcgatgc gtact 15 <210> 402 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.alic004 <400> 402 cagatgtcga aaggt 15 <210> 403 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.alic005 <400> 403 taggacgtta tggtt 15 <210> 404 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.glab001 <400> 404 ctggaatgca cccgg 15 <210> 405 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Probe C.glab003 <400> 405 tggcttggcg gcgaa 15 <210> 406 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Primer 1585Fw <400> 406 ttgtacacac cgcccgtc 18 <210> 407 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Primer 23BR <400> 407 ttcgcctttc cctcacggta ct 22 <210> 408 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Primer 23BFw <400> 408 agtaccgtga gggaaaggcg aa 22 <210> 409 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Primer 37R <400> 409 tgcttctaag ccaacatcct 20 <210> 410 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Primer MS37F <400> 410 aggatgttgg cttagaagca 20 <210> 411 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Primer MS38R <400> 411 cccgacaagg aatttcgcta cctt 24 <210> 412 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Primer fun463F <400> 412 gtaattggaa tgagtacaat 20 <210> 413 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Primer fun986R <400> 413 ctacgacggt atctgatcat 20 <210> 414 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Primer 520 R <400> 414 gccaaggcat ccacc 15 <210> 415 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> Primer 23S 750F <400> 415 agtagcggcg agcgaa 16 <210> 416 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> Primer 23S 750F (T) <400> 416 agtagtggcg agcgaa 16 <210> 417 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> Primer 970F <400> 417 aactggagga ccgaacc 17 <210> 418 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> Primer 930R <400> 418 awtttgcyga gttcctt 17 <210> 419 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Primer 2960R (T) <400> 419 gcttagatgc tttcagca 18 <210> 420 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Primer 2960RC <400> 420 gcttagatgc tttcagcg 18 <210> 421 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Control Probe BaP1-01 <400> 421 cacggtggat gccct 15 <210> 422 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Control Probe Bap1-03 <400> 422 agtagcggcg agcga 15 <210> 423 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Control Probe BaP1-06 <400> 423 gaccgatagt gaacc 15 <210> 424 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Control Probe BaP2-01 <400> 424 agaacctgaa accgt 15 <210> 425 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Control Probe BaP2-03 <400> 425 actggaggac cgaac 15 <210> 426 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Control Probe BaP2-04 <400> 426 agggaaacaa cccag 15 <210> 427 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Control Probe BaP3 <400> 427 gtaaacggcg gccgt 15 <210> 428 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> Position marker <400> 428 aaaaaaaaaa aaaaa 15

Claims (2)

하기 염기 서열을 포함한 핵산 분자로 이루어진 그룹중에서 선택된 분리된 핵산 분자:An isolated nucleic acid molecule selected from the group consisting of nucleic acid molecules comprising the following nucleotide sequences: AGGAGGAAGAGAAAG (P.anae003, 서열번호 186); 또는AGGAGGAAGAGAAAG (P.anae003, SEQ ID NO: 186); or GCGAAAGGAAAAGAG (P.anae004, 서열번호 187).GCGAAAGGAAAAGAG (P.anae004, SEQ ID NO: 187). 제 1항의 서열번호 186 또는 187의 염기 서열을 포함한 핵산 분자를 포함하는 펩토스트렙토코커스 언애어로비우스 균의 검출용 핵산 탐침이 고체 지지체에 고정된 DNA 칩.A DNA chip in which a nucleic acid probe for detecting Peptostreptococcus Ear Aerbyus bacteria, comprising a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 186 or 187 of claim 1, is immobilized on a solid support.
KR1020057014603A 2005-08-08 2003-05-09 DNA chips for Detection of Peptostreptococcus anarobius bacilli KR100577459B1 (en)

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