KR20050052416A - Functional health food product containing the extract of african ganoderma mushroom for protecting and improving viral diseases - Google Patents

Abstract

The present invention relates to a composition containing an extract of Ganoderma applanata. (Pers. Wallr) Pat .

African Ganoderma lucidum extract according to the present invention exhibits excellent antiviral activity, and can be used as an effective and safe health functional food for the prevention and improvement of viral diseases.

Description

Functional health food product containing the extract of African ganoderma mushroom for protecting and improving viral diseases}

The present invention relates to a composition containing an extract of Ganoderma applanata. (Pers. Wallr) Pat.), Which exhibits antiviral activity. It relates to a dietary supplement containing mushroom extracts.

As modern medicine advances, bacterial diseases are markedly suppressed by the development of antibiotics and preventive vaccines, but viral diseases are not yet conquered, but rather are reported to increase through air pollution and environmental pollution. However, only a few viral diseases in which prophylactic vaccines are developed, and fundamental treatments for many diseases, including viral flu, hepatitis, aseptic meningitis, and the like, have not yet been developed.

Enteroviruses are single-stranded (+) RNA viruses belonging to the family Picornaviridae . It is a representative human pathogen that causes aseptic meningitis, epidemic keratoconjunctivitis (Apollo's eye disease), and hand, foot and mouth disease in children and infants. In Japan, 5000-6000 cases (5804 cases in 1998) are isolated from enteroviral diseases every year, and sporadic cases of enteroviral cases have been reported in Korea since the early 1990s.

However, isolation and identification of viruses through cell culture for diagnosis in the event of disease (Kim et al. 1994. Journal of Korea Society of Virology 24: 77-86), or genetic diagnosis using RT-PCR methods are only used. Zoll et al. 1992. Journal of Clinical Microbiology 30: 160-165. Vaccine preparations aimed at defense against enteric viral diseases have not yet been developed.

Ganoderma Lucidum (FR. Karst ), a member of the Buloba excess, is called Bulchocho and perennial mushrooms, and is distributed in the northern part of the northern hemisphere temperate regions such as Korea, Japan, and China. It is a myrtle heartwood thickening mushroom that grows or grows on hardwoods or stumps of broad-leaved trees from summer to autumn. And enzymes that break down lignin. Ganoderma lucidum has no side effects, anti-cancer effect, coarse effect, anti-blood effect, sweetening effect, asthma suppression effect, immunity effect, interpolation ( It is effective in the treatment and prevention of more than 170 kinds of diseases, including the effect of 補肝, jeongjeong (强 精), diuretic (,), detoxification, anti-inflammatory (消炎), strong heart (심) effect It is widely used for medicinal purposes. Because of its excellent pharmacological action, Ganoderma lucidum and its extracts have been widely used as medicines and health functional foods for a long time.

African Ganoderma lucidum according to the present invention (Ganoderma applanata. (Pers. Wallr ) Pat.) Was bulrochogwa (Ganodermataceae), it belongs to the bulrocho in (Ganoderma), growing the parasitic like hardwood native to the wild state in alpine give way zone of Kenya It is a mushroom. The ecological feature of this area is the alpine region of 5000m above sea level, although it is a tropical region near the equator, it has an optimal environment for growing mushrooms. It grows on dead wood and stump of hardwood hardwood, and grows at right angle to stump. The shape is round in shape of a horseshoe. The surface is smooth and has the shape of a kidney, semicircle. The front is yellowish white at first, but grows from reddish brown to reddish brown. The back side is yellowish white and has a lot of bureaucracy. The stem is slightly curved with the same color as the surface of the lampshade. It is called an artist's hat because light spots show brown spots on the surface of white pores.

It is known to be effective in preventing memory loss, inhibiting aging, physiologically active cell activity, anti-inflammatory, antibacterial action, blood pressure strengthening action, anti-allergy, antihistamine, anti-male hormone action. It has anti-cancer effects, and the extract has also been proved by clinical trials as having an excellent therapeutic and preventive effect against liver cancer, lung cancer and many other cancers.

However, the antiviral activity of the African Ganoderma lucidum has not been studied.

Kenya is known to have more than 50% of residents living with viral diseases. The inventors of the present invention focus on the fact that the inhabitants of the region where African Ganoderma lucidum grows wildly have a lower mortality rate due to viral disease than other residents, and thus, the antiviral activity of the mushrooms used as a traditional treatment of the inhabitants As a result of the study, it was confirmed that the African Ganoderma lucidum extract exhibited excellent antiviral activity, particularly against intestinal viruses, and completed the present invention.

The present invention provides a dietary supplement for the prevention and improvement of viral diseases containing African Ganoderma lucidum extract, which exhibits excellent antiviral activity.

In order to achieve the above object, the present invention contains an African Ganoderma lucidum extract as an active ingredient, and provides a health functional food effective for the prevention and improvement of intestinal viral diseases.

The health functional food is characterized in that the powder, granules, tablets, capsules or beverage form.

Hereinafter, the present invention will be described in detail.

African Ganoderma lucidum extract of the present invention, after crushing and grinding the dried African Ganoderma lucidum, about 5 to 25 times the weight (kg), preferably about 10 to 15 times the water, 1 to 4 carbon atoms Lower alcohols or their mixed solvents having a mixing ratio (kg / l) of about 1: 0.1 to 1:10, preferably 1: 0.2 to 1: 5, are extracted at 50 to 100 ° C., preferably 70 to 80 ° C. Soluble extract according to water, lower alcohol, or a mixed solvent thereof is extracted, concentrated under reduced pressure and freeze-dried by extraction methods such as solvent extraction and hot water extraction for about 1 hour to 1 day, preferably 2 hours to 4 hours at a temperature. It can be obtained by.

The present invention provides a health functional food comprising an extract of an African Ganoderma lucidum mushroom and a food supplement acceptable food supplement additive exhibiting an effect of preventing and improving intestinal viral diseases.

Compositions comprising the extracts of the present invention can be used in a variety of food and beverages for the prevention and improvement of viral diseases. Examples of foods to which the extract of the African Ganoderma lucidum mushroom can be added include various foods, for example, candy, chocolate, beverages, gum, tea, vitamin complexes, health functional foods, and the like, and powders, granules, tablets and capsules. Or in the form of a beverage.

At this time, the amount of the extract in the food or beverage, in the case of the health functional food composition of the present invention can generally be added to 0.01 to 50% by weight, preferably 0.1 to 20% by weight of the total functional food composition, In the case of 0.02 to 10 g, preferably 0.3 to 1 g, based on 100 ml.

The health functional beverage composition of the present invention is not particularly limited in the liquid component except for containing the extract as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates, etc. as additional ingredients, as in general beverages. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; Conventional sugars such as polysaccharides such as dextrin, cyclodextrin and the like and sugar alcohols such as xylitol, sorbitol, erythritol and the like. The proportion of such natural carbohydrates is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention.

Other flavors may be advantageously used natural flavors (tautin, stevia extracts (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.). The composition of the present invention is a variety of nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese, chocolate), pectic acid and salts thereof, alginic acid and salts thereof, Organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, etc. Other compositions of the present invention may be used in natural fruit juices and fruit juice beverages and vegetable beverages. The components may be used independently or in combination The ratio of such additives is not critical but is zero per 100 parts by weight of the composition of the present invention. It is generally selected from the range of about 20 parts by weight.

 African Ganoderma lucidum extract itself of the present invention has little toxicity and side effects, so can be used with confidence even for long-term administration for the purpose of prevention.

The present invention is described in more detail based on the following examples and examples, but the present invention is not limited to the examples or experimental examples.

Example 1 Preparation of African Ganoderma Lucidum Mushroom Extract

After drying 2.1 kg of dried African Ganoderma lucidum ( Ganoderma applanata. (Pers. Wallr) Pat. Africa Kenya) was cut and powdered, 5 L of methanol was added and the mixture was deposited at 70 ° C. for 2 hours. The methanol solution extracted from the sample was filtered through a filter paper (filter No. 1, Whatama, USA), concentrated under reduced pressure using a vacuum condenser (EYELA, Japan), and lyophilized to extract 85 g of African Ganoderma lucidum extract. Got.

Example 2. Preparation of African Ganoderma Lucidum Mushroom Extract

50 g of dried African Ganoderma lucidum was extracted by hot water using an electronic shaker (Heating mentle, manufactured by M-tops) for more than 3 hours in 1000 ml of distilled water. The extract was filtered, concentrated under reduced pressure using a vacuum condenser, and freeze-dried to obtain an African Ganoderma lucidum extract with a dry weight of 20.8 g.

Experimental Example 1. Intestinal virus inhibitory activity of Ganoderma lucidum extract

The intestinal virus of the African Ganoderma lucidum extract obtained in Example 1 was tested using a cell viability test (MTT Assay), respectively. The MTT test is a colorimetric measurement of the activity of mitochondrial dehydrogenase, which is mainly used to determine mitochondrial redox potential or cell viability (Mosmann et al. 1983).

For this experiment, RD-A cell line (Rhabdomysarcoma, National Institute of Health pediatric machine virus, October 18, 2002) was used, and enterovirus as coxsackie virus (Coxsackie B Virus 100 TCID50 (10 ^-5 / ml), Hereinafter, CB2 virus) was used (National Institutes of Pediatric Virology, October 18, 2002).

RD-A cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) with 10% FBS (Fetal Bovine Serum). When the cells grew properly, the cells were washed three times with PBS (Phosphate Buffered Saline) and then PBS was removed. Trysin was treated to allow cells to fall off the culture plate (culture plate) to be separated by one colony, followed by centrifugation (1000 rpm / 10 minutes) to form pellets. A certain amount of DMEM was added, mixed well, and then transferred to a culture plate. An African Ganoderma lucidum extract solution of Example 1 was prepared by diluting RD-A cells (3 × 10 ⁵ cells / well) sequentially by four-fold from the same concentration of CB2 virus (10 ⁻⁵ ) and a reference concentration of 30 ug / ml. Was added to a microtiter plate with 96 wells and incubated at 37 ° C. in a 5% CO ₂ incubator for 3-4 days. At this time, both the cell control group (hereinafter referred to as CC) and the virus control group (hereinafter referred to as VC) were added with PBS solution instead of the extract of the present invention, and the cell control group was not infected with the virus. MTT reagent (Sigma, USA); 3- [4,5-dimethylthiazol-2-yl] -2,5-diphenyl tetrazolium bromide (3- [4,5-dimethylthiazol-2-yl] -2,5-diphenyl tetrazolium bromide) After dissolving in PBS (phosphate buffered saline) at a concentration of mg / ㎖ was used by sterile filtration with a 0.22um membrane filter (membrane filter). 50 μl of MTT (0.5 mg / ml) was added to each well, incubated at 37 ° C. for 3 hours, and then 10 μl of 0.1M HCl was added to stop MTT-formazan formation. Live cells with active mitochondria break down the tetrazolium ring to form a dark blue formazan, so to dissolve it, add 100 μl of 10% sodium dodecylsulfate (SDS) overnight. The MTT-formazan was completely dissolved, and then absorbance (OD) was measured at a wavelength of 570 nm.

When the virus penetrates the cells, the cells are killed and the OD value is significantly decreased during MTT staining. However, cells containing drugs will maintain a constant OD value at a certain concentration by antiviral activity.

No. Titer Treatment Average Absorbance (Ave.OD) One Cell control CC 1.178 2 Virus control group (10 ^-5 ) VC 0.264 3 Extract of Reference Concentration 30 µg / ml 0.062 4 4 ^(-1) of the reference concentration 7.5 µg / ml 0.135 5 4 ^(-2) of the reference concentration 1.8 µg / ml 1.706 6 4 ^(-3) of the reference concentration 450ng / ml 2.048 7 4 ^(-4) of the reference concentration 110ng / ml 1.326 8 4 ^(-5) of the reference concentration 20ng / ml 1.773 9 4 ^(-6) of the reference concentration 5ng / ml 1.038 10 4 ^(-7) of the reference concentration 1.25ng / ml 1.508 11 4 ^(-8) of the reference concentration 312 pg / ml 1.794 12 4 ^(-9) of the reference concentration 78 pg / ml 0.342

CC: Cell control without added virus and extract.

VC: Virus control group with only virus but no extract.

Experimental results, as shown in Table 1 and Figure 1, in the case of cells without addition of the extract of the present invention, the OD value is significantly decreased during MTT staining, the cells added with the extract of the present invention is the anti-ageh OD values were kept constant at constant concentrations by viral activity.

The results of three replicates showed that the extract of the African Ganoderma lucidum mushroom (Example 1) showed antiviral activity at a concentration of 1.8 ug / ml-0.4 pg / ml against the CB2 virus.

Experimental Example 2. Toxicity Test

In order to test the toxicity of the African Ganoderma lucidum extract of the present invention, animal experiments were performed.

The African Ganoderma of the present invention is divided into three groups of 25 ± 5g of ICR mice (central laboratory animals) and 235 ± 10g of SPF Sprague Dawley (Biogenomics) rats, respectively, divided into three groups. Mushroom extract (Example 1) Toxicity was observed for 24 hours after intraperitoneal administration at a dose of 20 mg / kg, 10 mg / kg, 1 mg / kg, respectively.

As a result, no deaths were observed in all three groups, and no significant symptoms were found in weight gain and feed intake. Therefore, it was confirmed that the African Ganoderma lucidum extract of the present invention is a safe drug.

African Ganoderma lucidum extract of the present invention can be administered in the following formulations, the following formulation examples are merely to illustrate the invention, whereby the content of the present invention is not limited.

Formulation Example 1 Preparation of Healthy Food

1000 mg of extract of Example 1

Vitamin mixture proper amount

70 μg of Vitamin A Acetate

Vitamin E 1.0 mg

Vitamin B ₁ 0.13 mg

Vitamin B ₂ 0.15 mg

Vitamin B ₆ 0.5 mg

0.2 μg of vitamin B ₁₂

Vitamin C 10 mg

10 μg biotin

* Nicotinamide 1.7 mg

Folate 50 ㎍

Calcium Pantothenate 0.5mg

Mineral mixture

Ferrous Sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium Citrate 90 mg

Calcium Carbonate 100 mg

Magnesium chloride 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixtures is mixed with a component suitable for a health food in a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health food manufacturing method. The granules may be prepared and used for preparing a health food composition according to a conventional method.

Formulation Example 2 Preparation of Healthy Drinks

1000 mg of extract of Example 1

Citric acid 1000 mg

100 g oligosaccharides

Plum concentrate 2 g

1 g of taurine

Add 900 ml of purified water

After mixing the above components in accordance with a conventional healthy beverage production method, and stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed sterilization and then refrigerated and stored in the present invention For the preparation of healthy beverage compositions.

African Ganoderma lucidum extract according to the present invention exhibits antiviral activity and can be used as a health functional food useful for the prevention and improvement of various viral diseases.

1 is a diagram showing the antiviral activity of the extract from the African Ganoderma lucidum against Koksagi virus.

Claims (2)

A dietary supplement for the prevention and improvement of intestinal viral diseases comprising African Ganoderma lucidum extract and food acceptable food supplement additives. The dietary supplement of claim 1 in the form of a powder, granule, tablet, capsule or beverage.