KR20050042247A - Genes that code for homeostasis proteins and for adaptation proteins - Google Patents

Abstract

The invention relates to novel nucleic acid molecules, to their use for constructing genetically improved microorganisms and to methods for producing fine chemicals, in particular, amino acids by using these genetically modified microorganisms.

Description

Genes that encode homeostatic and adaptive proteins {Genes that Code for Homeostasis Proteins and for Adaptation Proteins}

Certain products and by-products of naturally occurring metabolic processes in cells are used in a variety of industries, including the food, animal feed, cosmetic and pharmaceutical industries. These molecules, collectively called "fine chemicals", include organic acids, proteinogenic and nonproteinogenic amino acids, nucleotides and nucleosides, lipids and fatty acids, diols, carbohydrates, aromatic compounds, vitamins, cofactors and enzymes. They are most conveniently produced by large-scale cultivation of bacteria, each developed to produce and secrete large quantities of the desired molecules. A particularly suitable organism for this purpose is the Corynebacterium glutamicum (Corynebacterium glutamicum) pathogenic bacteria in the Gram-positive. Through strain selection, a number of mutant strains have been developed that produce a variety of compounds of interest. However, the selection of improved strains for the production of certain molecules is a time consuming and difficult process.

<Overview of invention>

The present invention provides novel nucleic acid molecules that can be used to identify or classify Corynebacterium glutamicum or bacterial species associated therewith. Corynebacterium glutamicum is a Gram-positive aerobic bacterium widely used in industry for the large scale production of many fine chemicals, the decomposition of hydrocarbons (eg for petroleum spills) and the oxidation of terpenoids. Thus, nucleic acid molecules can be used to identify microorganisms that can be used, for example, to produce fine chemicals through fermentation processes. Although Corynebacterium glutamicum itself is non-pathogenic, it is associated with other Corynebacterium species, such as Corynebacterium diphteriae (pathogen of diphtheria), a major pathogen in humans. Thus, for example, the ability to identify the presence of Corynebacterium species is of clinical significance in the diagnostic field. Moreover, the nucleic acid molecule can serve as a reference point for mapping the genome of Corynebacterium glutamicum or the genome of related organisms.

These novel nucleic acid molecules encode proteins called herein homeostasis and adaptation (HA) proteins. These HA proteins may, for example, perform functions associated with maintaining homeostasis in Corynebacterium glutamicum, or with the ability to allow this microorganism to adapt to other environmental conditions. Availability of cloning vectors for use in Corynebacterium glutamicum as disclosed in US Pat. No. 4,649,119 (Sinskey et al.), And Corynebacterium glutamicum and related Brevibacterium species (Eg, lactofermentum) genetic engineering techniques (Yoshihama et al., J. Bacteriol. 162: 591-597 (1985); Katsumata et al., J. Bacteriol. 159: 306-311 (1984); And because of the availability of Santamaria et al., J. Gen. Microbiol. 130: 2237-2246 (1984)), the nucleic acid molecules of the present invention allow the organism to produce one or more fine chemicals more efficiently and more efficiently. May be used to genetically engineer the organism. The productivity or efficiency of fine chemical production can be improved directly or indirectly by genetic engineering of the present invention.

There are a number of mechanisms that can modify the HA protein of the present invention and directly affect the yield, productivity, and / or efficiency of fine chemical production from Corynebacterium glutamicum containing the modified protein. . By genetically modifying an enzyme that modifies an aromatic or aliphatic compound to increase or decrease the activity or number of the enzyme, the production of one or more microchemicals that are modified or degraded products of the present invention can be controlled. Thus, enzymes involved in the metabolism of inorganic compounds provide molecules important for biosynthesis of fine chemicals such as amino acids, vitamins and nucleic acids (eg, phosphorus, sulfur and nitrogen molecules). By varying the activity or number of these enzymes in Corynebacterium glutamicum, the conversion of these inorganic compounds can be increased (or using alternative compounds) to improve the incorporation of these inorganic atoms into the fine chemicals. . Genetic engineering of Corynebacterium glutamicum enzymes associated with universal cellular processes involves the direct modification of many of these enzymes involved in the synthesis or secretion of microchemicals (eg, amino acids) or microchemicals. Therefore, the productivity of fine chemicals can be directly improved. In addition, the activity or number regulation of cellular proteases may have a direct effect on the production of fine chemicals because many proteases can degrade the enzymes involved in the production or degradation of fine chemicals or fine chemicals.

The enzymes mentioned above involved in the modification and / or degradation of aromatic or aliphatic compounds, the universal biocatalysis, the metabolism of inorganic compounds or the proteolysis of proteins, due to their activity, have caused various in vitro industries. Preferred fine chemicals in the field. In Corynebacterium glutamicum it is possible to change the copy number of the genes for one or more of these enzymes to increase the number of proteins produced by the cells, thereby corynebacterium glutamicum or related bacteria May increase the potential yield or efficiency of the protein production from large-scale cultures.

In addition, modification of the HA protein of the present invention may indirectly affect the yield, productivity and / or efficiency of fine chemical production from Corynebacterium glutamicum containing the modified protein. For example, by regulating the activity and / or number of proteins involved in the construction or rearrangement of the cell wall, it is possible to alter the structure of the cell wall itself so that the cells are better able to tolerate mechanical and other stresses present in large-scale fermentation cultures. Can be. In addition, large-scale cultivation of Corynebacterium glutamicum requires significant cell wall production. Controlling the activity or number of cell wall biosynthesis enzymes or degrading enzymes can speed up cell wall biosynthesis, which in turn increases the growth rate in the culture of the microorganisms, thereby increasing the number of cells producing the desired fine chemicals.

By modifying the HA enzyme of the present invention, it can indirectly affect the yield, productivity or efficiency of producing one or more microchemicals from Corynebacterium glutamicum. For example, many universal enzymes of Corynebacterium glutamicum can have a significant impact on the overall cellular processes (eg, regulatory processes), which have a significant effect on the metabolism of fine chemicals.

Thus, proteases (ie, enzymes that modify or degrade aromatic or aliphatic compounds that may be toxic) increase the viability of Corynebacterium glutamicum. This protease aids in the selective removal of misfolded or misregulated proteins, for example, proteins that may arise under relatively stressful environmental conditions seen in large fermentation cultures. By modifying these proteins, it is further possible to increase the activity of the protein and increase the viability of Corynebacterium glutamicum in culture. Proteins for the modification or degradation of aromatic / aliphatic compounds not only eliminate the toxicity of these waste compounds (either as contaminants in the culture medium or as waste from the cells themselves), but also when the optimal carbon source in the culture is limited. It allows cells to use alternative carbon sources. By increasing their number and / or activity, it is possible to increase the survival rate of Corynebacterium glutamicum in culture. The inorganic compound metabolic protein of the present invention is essential for the overall viability of the cell, in particular because it supplies the cell with inorganic molecules essential for the synthesis of all proteins and nucleotides. Increasing the number of viable cells producing one or more microchemicals in a large scale culture will at the same time increase the yield, productivity and / or efficiency of the microchemical production in the culture.

The present invention relates to a protein referred to herein as a HA protein (e.g., to a function related to the maintenance of homeostasis in Corynebacterium glutamicum or to the ability of this microorganism to adapt to various environmental conditions). Provided are novel nucleic acid molecules encoding. Nucleic acid molecules encoding HA proteins are referred to herein as HA nucleic acid molecules. In a preferred embodiment, the HA protein is involved in cell wall biosynthesis or rearrangement of Corynebacterium glutamicum, metabolism of inorganic compounds, modification or degradation of aromatic or aliphatic compounds, or enzymatic activity of Corynebacterium glutamicum Or proteolytic activity. Examples of such proteins are those encoded by the genes listed in Table 1.

Accordingly, one aspect of the present invention provides for the detection of isolated nucleic acid molecules (eg, cDNA), and HA coding nucleic acids (eg, DNA or mRNA) comprising nucleotide sequences encoding HA proteins or biologically active portions thereof. Or nucleic acid fragments suitable as primers or hybridization probes for amplification. In a particularly preferred embodiment, the isolated nucleic acid molecule comprises a coding region of any of the nucleotide sequences described in Appendix A, or any of these nucleotide sequences, or its complement. In another preferred other embodiment, the isolated nucleic acid molecule encodes any amino acid sequence described in Appendix B. In addition, preferred HA proteins of the present invention preferably have at least one of the HA activities described herein.

Appendix A, hereinbelow, defines nucleic acid sequences in the Sequence Listing with sequence modifications at the relevant positions listed in Table 1.

Appendix B defines herein the polypeptide sequences in the Sequence Listing with the sequence modifications at the relevant positions listed in Table 1.

In other embodiments, the isolated nucleic acid molecules are at least 15 nucleotides in length and hybridize with nucleic acid molecules comprising the nucleotide sequence of Appendix A under stringent conditions. Preferably, the isolated nucleic acid molecule corresponds to a naturally occurring nucleic acid molecule. More preferably, the isolated nucleic acid encodes the HA protein of naturally occurring Corynebacterium glutamicum or a biologically active portion thereof.

Another aspect of the invention relates to vectors (eg, recombinant expression vectors) comprising the nucleic acid molecules of the invention, and host cells into which such vectors are introduced. In one embodiment, the HA protein is prepared using host cells cultured in a suitable medium. The HA protein can then be isolated from the medium or host cell.

Another aspect of the invention relates to a genetically modified microorganism in which the HA gene is introduced or the HA gene is modified. In one embodiment, the genome of the microorganism was modified by introducing one or more nucleic acid molecules of the invention encoding a mutant HA sequence as a transgene. In other embodiments, the endogenous HA gene in the microbial genome has been modified (eg, functionally disrupted) by homologous recombination with the modified HA gene. In a preferred embodiment, the microorganism belongs to the genus Corynebacterium or Brevibacterium, with Corynebacterium glutamicum particularly preferred. In a preferred embodiment, the microorganism is also used for the production of the desired compound, such as amino acids (particularly lysine).

Another preferred embodiment relates to a host cell having at least one nucleic acid molecule described in Appendix A. Such host cells can be prepared in a variety of ways known to those skilled in the art. Host cells can be transfected, for example, with vectors carrying the various nucleic acid molecules of the invention. However, it is also possible to use vectors for introducing one nucleic acid molecule of the invention into a host cell, so that multiple vectors can be used simultaneously or sequentially. Thus, it is possible to produce host cells bearing a myriad (up to several hundred) nucleic acid sequences of the present invention. Such accumulation can often produce a host cell's superadditive effect on fine chemical productivity.

Another aspect of the invention relates to an isolated HA protein or portion thereof, eg a biologically active portion thereof. In a preferred embodiment, the isolated HA protein or portion thereof may be involved in maintaining homeostasis in Corynebacterium glutamicum or perform a function associated with adaptation of this microorganism to other environmental conditions. In another preferred embodiment, the isolated HA protein, or portion thereof, is directed to Appendix B so that it can participate in the maintenance of homeostasis in Corynebacterium glutamicum or perform a function related to adaptation of this microorganism to other environmental conditions. There is sufficient homology with the amino acid sequence or part thereof.

Moreover, the present invention relates to an isolated HA protein preparation. In a preferred embodiment, the HA protein comprises the amino acid sequence of Appendix B. In another preferred embodiment, the invention relates to an isolated full length protein that is essentially homologous to the complete amino acid sequence of Appendix B (coded by the open reading frame of Appendix A).

HA polypeptides or biologically active portions thereof may be functionally linked with polypeptides other than HA polypeptides to generate fusion proteins. In a preferred embodiment the activity of this fusion protein is different from the activity of only HA proteins, and in other preferred embodiments the fusion protein is yielded, productivity and / or of desired microchemical production from Corynebacterium glutamicum. Increase the efficiency. In a particularly preferred embodiment, said fusion protein is integrated into a host cell to regulate the production of the desired compound by said cell.

Another aspect of the invention relates to a method for producing a fine chemical. This method involves culturing cells containing vectors expressing HA nucleic acid molecules of the present invention to produce fine chemicals. In a preferred embodiment, the method also comprises the step of transfecting the cells with a vector expressing the HA nucleic acid resulting in a cell containing said vector. In another preferred embodiment, the method also includes recovering the fine chemical from the culture. In a preferred embodiment, the cells belong to the genus Corynebacterium or Brevibacterium.

Another aspect of the invention relates to a method of regulating molecular production from microorganisms. The method includes contacting the cell with a substance that modulates HA protein activity or HA nucleic acid expression such that the cell related activity is changed compared to the same activity in the absence of the substance. In a preferred embodiment, the cells are subjected to at least one of the Corynebacterium glutamicum processes associated with cell wall biosynthesis or rearrangement, metabolism of inorganic compounds, modification or degradation of aromatic or aliphatic compounds, or enzymatic activity or proteolytic activity. Is adjusted. A substance that modulates HA protein activity is, for example, to stimulate HA protein activity or HA nucleic acid expression. Examples of agents that stimulate HA protein activity or HA nucleic acid expression include nucleic acids encoding small molecules, active HA proteins, and HA proteins and introduced into cells. Examples of substances that inhibit HA activity or HA expression include small molecules and antisense HA nucleic acid molecules.

Another aspect of the invention is a method of regulating the yield of a desired compound from a cell, comprising introducing a HA wild type gene or HA mutant gene into the cell such that it is maintained on a separate plasmid or integrated into the genome of the host cell. It is about. Integration into the genome occurs either randomly or by homologous recombination, which allows natural genes to be replaced by integrated copies, thereby allowing the production of the desired compound from the cell to be regulated. In a preferred embodiment, the yield is increased. In another preferred embodiment, the chemical is a fine chemical, in a particularly preferred embodiment an amino acid. In a particularly preferred embodiment, said amino acid is L-lysine.

The present invention relates to cell wall biosynthesis or rearrangement, metabolism of inorganic compounds, modification or degradation of aromatic or aliphatic compounds, or enzymatic activity or proteolytic activity of Corynebacterium glutamicum in Corynebacterium glutamicum. HA nucleic acids and HA protein molecules are provided. Molecules of the present invention regulate the production of microchemicals directly from microorganisms such as Corynebacterium glutamicum (e.g., overexpress or inhibit the activity of proteins involved in the production of microchemicals (e.g. enzymes)). Optimized or used to directly affect the yield, productivity and / or efficiency of fine chemical production from modified Corynebacterium glutamicum, or indirectly, but nevertheless yield, productivity of desired compound production. And / or modulating the production of fine chemicals in a manner that increases efficiency (e.g., by controlling the copy number or activity of proteins that modify or degrade aromatic or aliphatic compounds from Corynebacterium glutamicum). It can increase the viability of Corynebacterium glutamicum and thereby increase productivity in large scale cultures. Notes) are available to you. Aspects of the invention are described in more detail below.

I. Fine Chemicals

The term "fine chemical" is known in the art and includes molecules produced by organisms used in a variety of industries, such as, but not limited to, the pharmaceutical, agricultural and cosmetic industries. Such compounds include organic acids such as tartaric acid, itaconic acid and diaminopimelic acid, proteinogenic and nonproteinogenic amino acids, purine and pyrimidine bases, nucleosides and nucleotides (eg, Kuninaka, A. (1996) Nucleotides and related compounds, pp. 561-612, Biotechnology Vol. 6, Rehm et al., Editors.VCH: Weinheim and its references), lipids, saturated and unsaturated fatty acids (eg, arachidonic acid), Diols (eg propanediol and butanediol), carbohydrates (eg hyaluronic acid and trehalose), aromatic compounds (eg aromatic amines, vanillin and indigo), vitamins and cofactors (Ullmann's Encyclopedia of Industrial Chemistry, Vol. A27, "Vitamins", pp. 443-613 (1996) VCH: Weinheim and its references, and Ang, AS, Niki, E. and Packer, L. (1995) "Nutrition, Lipids, Health, and Disease "Proceedings of the UNESCO / Confederation of Scien tific and Technological Associations in Malaysia and the Society for Free Radical Research-Asia, held Sept. 1-3, 1994 at Penang, Malaysia, AOCS Press, (1995)), enzymes, and Gutcho (1983) ), Chemicals by Fermentation, Noyes Data Corporation, ISBN: 0818805086 and all other compounds described therein. The metabolism and use of certain fine chemicals are described in more detail below.

A. Amino Acid Metabolism and Uses

Amino acids make up the basic structural unit of every protein and are essential for normal cellular function. The term "amino acid" is known in the art. The proteinogenic amino acids in which there are 20 species act as structural units for the proteins to which they are bound by peptide bonds, whereas nonproteinogenic amino acids (hundreds of known species) are largely not found in proteins (Ulmann's Encyclopedia of Industrial Chemistry, Vol. A2, pp. 57-97 VCH: Weinheim (1985). Although L-amino acids are generally the only form found in naturally occurring proteins, amino acids may exist in D or L structures. Features of proteins produced amino acid biosynthesis and degradation pathways of each of the 20 kinds are well identified in both prokaryotic and eukaryotic cells (see, e.g., [Stryer, L. Biochemistry, 3 ^rd edition, pp. 578-590 ( 1988). Because of the complexity of biosynthesis, amino acids called “essential” amino acids (histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine) are required to be consumed as food. "Non-essential" amino acids (alanine, arginine, asparagine, aspartate, cysteine, glutamate, glutamine, glycine, proline, serine and tyrosine). Higher animals can synthesize some of the essential amino acids, but the essential amino acids must be supplied from food to effect normal protein synthesis.

In addition to the function of amino acids in protein biosynthesis, these amino acids are themselves interesting chemicals, and many amino acids have been found to be used in a variety of applications in the human food, animal feed, chemical, cosmetic, agricultural and pharmaceutical industries. Lysine is an important amino acid not only in human nutrition but also in the nutrition of monogastric animals such as poultry and swine. Glutamate is most commonly used as a flavourant additive (monosodium glutamate, MSG), and aspartate, phenylalanine, glycine and citrate are also widely used throughout the food industry. Glycine, L-methionine and tryptophan are all used in the pharmaceutical industry. Glutamine, valine, leucine, isoleucine, histidine, arginine, proline, serine and alanine are used in both the pharmaceutical and cosmetic industries. Threonine, tryptophan and D / L-methionine are widely used as animal feed additives (Leuchtenberger, W. (1996) Amino acids-technical production and use, pp. 466-502 in Rehm et al. (Editors.) Biotechnology Vol. 6, chapter 14a, VCH: Weinheim). These amino acids also include synthetic amino acids and proteins such as N-acetylcysteine, S-carboxymethyl-L-cysteine, (S) -5-hydroxytryptophan, and Ulmann's Encyclopedia of Industrial Chemistry, Vol. A2, pp. 57-97, VCH: Weinheim, 1985, have been found to be suitable as precursors for the synthesis of other materials.

The biosynthesis of these natural amino acids in organisms capable of producing natural amino acids, such as bacteria, is well characterized (a review of bacterial amino acid biosynthesis and its control is described in Umbarger, HE (1978) Ann. Rev. Biochem. : 533-606). Glutamate is synthesized by reductive amination of α-ketoglutarate, the intermediate product of the citric acid cycle. Glutamine, proline and arginine are each produced successively from glutamate. Biosynthesis of serine occurs in a three step process, starting with 3-phosphoglycerate (an intermediate product of its action), producing serine after oxidation, transamination and hydrolysis steps. Cysteine and glycine are each produced from serine, specifically cysteine is produced by condensation of homocysteine and serine, and glycine is transferred to tetrahydrofolate by the side chain β-carbon atoms in a reaction catalyzed by serine transhydroxymethylase. Is generated. Phenylalanine and tyrosine are synthesized from erythros 4-phosphate and phosphoenolpyruvate, which are glycolysis and pentose phosphate pathway precursors in a nine-step biosynthetic pathway that is branched only in the final two stages after the synthesis of prephenate. Tryptophan is also produced from these two starting molecules, but its synthesis is an 11 step pathway. Tyrosine may also be prepared from phenylalanine in a reaction catalyzed by phenylalanine hydroxylase. Alanine, valine and leucine are each biosynthetic products derived from pyruvate, the final product of glycolysis. Aspartate is produced from oxaloacetate, the intermediate product of the citric acid cycle. Asparagine, methionine, threonine and lysine are produced by the conversion of aspartate, respectively. Isoleucine is produced from threonine. Histidine is produced from 5-phosphoribosyl-1-pyrophosphate, an activated sugar in a complex nine-step pathway.

The amount of amino acids in excess of the amount required protein biosynthesis of the cell can not be stored, is decomposed and instead is provided as an intermediate for the major metabolic pathways of the cell (study are described in [Stryer, L. Biochemistry 3 ^rd edition, Chapter 21 "Amino Acid Degradation and the Urea Cycle" pp. 495-516 (1988)). Although cells can convert unwanted amino acids into useful metabolic intermediate products, amino acid production is a lossy process given the energy, precursor molecules and enzymes necessary for their synthesis. Thus, it is not surprising that the presence of a particular amino acid modulates the biosynthesis of the amino acid by feedback inhibition that slows or completely stops its own production (a review of the feedback mechanism of the amino acid biosynthetic pathway is described in Stryer, L. Biochemistry 3 ^rd edition, Chapter 24 "Biosynthesis of Amino Acids and Heme" pp. 575-600 (1988). Thus, the yield of certain amino acids is limited by the amount of amino acids present in the cell.

B. Metabolism and Uses of Vitamins, Cofactors and Nutritional Supplements

Vitamins, cofactors, and nutrients make up another group of molecules. These molecules can be easily synthesized by other organisms, such as bacteria, but higher animals have to take them because they have lost this synthetic ability. These molecules are themselves bioactive materials or precursors of biologically active materials that can act as electron carriers or intermediates in various metabolic pathways. In addition to their nutritional value, these compounds have significant industrial value as pigments, antioxidants and catalysts, or as adjuvants of other processes (a review of the structure, activity and industrial use of these compounds is given, for example, in Ullman's Encyclopedia of Industrial Chemistry, "Vitamins" Vol. A27, pp. 443-613, VCH: Weinheim, 1996). The term "vitamin" is known in the art and includes nutrients necessary for the normal functioning of the organism but which the organism cannot synthesize by itself. The vitamin group may also include cofactors and nutritional compounds. The term "cofactor" includes nonproteinogenic compounds that are essential for normal enzymatic activity to occur. Such compounds may be organic or inorganic, and the cofactor molecules of the invention are preferably organic. The term “nutritive agent” includes food additives that promote health in plants and animals, especially humans. Examples of such molecules are vitamins, antioxidants and certain lipids (eg polyunsaturated fatty acids).

The biosynthesis of these molecules occurring in organisms capable of producing them, such as bacteria, is well characterized (Ullman's Encyclopedia of Industrial Chemistry, "Vitamins" Vol. A27, pp. 443-613, VCH: Weinheim, 1996; Michal, G. (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley &Sons; Ong, AS, Niki, E. & Packer, L. (1995) "Nutrition, Lipids, Health, and Disease" Proceedings of the UNESCO / Confederation of Scientific and Technological Associations in Malaysia, and the Society for Free Radical Research-Asia, held Sept. 1-3, 1994 at Penang, Malaysia, AOCS Press: Champaign, IL X, 374 S).

Thiamine (vitamin B ₁ ) is produced by chemical coupling of pyrimidine and thiazole residues. Riboflavin (vitamin B ₂ ) is synthesized from guanosine-5'-triphosphate (GTP) and ribose-5'-phosphate. Riboflavin is then used for the synthesis of flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). Compound groups collectively referred to as "vitamin B ₆ " (eg, pyridoxine, pyridoxamine, pyridoxal 5'-phosphate and commercial pyridoxine hydrochloride) are all typical structural units of 5-hydroxy-6-methyl Derivatives of pyridine. Pantothenate (pantothenic acid, (R)-(+)-N- (2,4-dihydroxy-3,3-dimethyl-1-oxobutyl) -β-alanine) is produced by chemical synthesis or fermentation Can be. The final stage of biosynthesis of pantothenate consists of ATP-induced condensation of β-alanine and pantosan. Biosynthesis steps for converting to pantosan, biosynthesis steps for converting to β-alanine, and enzymes involved in condensation to pantothenic acid are known. The metabolic active form of pantothenate is coenzyme A, the biosynthesis for coenzyme A proceeds in a five step enzymatic reaction. Pantothenate, pyridoxal 5'-phosphate, cysteine and ATP are precursors of coenzyme A. These enzymes not only catalyze the formation of pantothenate, but also (R) -pantosan, (R) -pantolactone, (R) -panthenol (provitamin B ₅ ), pantethene (and derivatives thereof) and coenzyme A Also catalyzes the production of.

Biosynthesis of biotin from pimeloyl-CoA, a precursor molecule in microorganisms, has been studied in detail and various related genes have been identified. A number of corresponding proteins have also been found to be involved in Fe cluster synthesis and these proteins belong to the class of nifS proteins. Lipoic acid is derived from octanoic acid and acts as a coenzyme in energy metabolism, which is a component of the pyruvate dehydrogenase complex and α-ketoglutarate dehydrogenase complex in energy metabolism. Folates are all a group of substances derived from folic acid, which are derived from L-glutamic acid, p-aminobenzoic acid and 6-methylphtherine. Biosynthesis of folic acid and its derivatives starting from the metabolic intermediate products of bioconversion of 5'-triphosphate (GTP), L-glutamic acid and p-aminobenzoic acid has been studied in detail in certain microorganisms.

Corinoids (eg cobalamin and in particular vitamin B ₁₂ ) and porphyrins belong to the group of chemicals distinguished by the tetrapyrrole ring system. The biosynthesis of vitamin B ₁₂ is so complex that its features are not fully characterized, but a number of enzymes and substrates are known. Nicotinic acid (nicotinate) and nicotinamide are pyridine derivatives, also referred to as "niacin". Niacin is an important coenzyme NAD (nicotinamide adenine dinucleotide), NADP (nicotinamide adenine dinucleotide phosphate) and their reduced precursors.

Although some of these chemicals, such as riboflavin, vitamin B ₆ , pantothenate and biotin are produced by large-scale cultivation of microorganisms, the industrial scale production of these compounds relies primarily on cell-independent chemical synthesis. Only vitamin B ₁₂ is produced entirely by fermentation due to the complexity of the synthesis of vitamin B ₁₂ . In vitro methods require significant inputs of material and time, and often expensive.

C. Metabolism and Uses of Purines, Pyrimidines, Nucleosides, and Nucleotides

Purine and pyrimidine metabolic genes and their corresponding proteins are important targets for the treatment of tumor diseases and viral infections. The term "purine" or "pyrimidine" includes nitrogen containing bases that are components of nucleic acids, coenzymes and nucleotides. The term "nucleotide" includes the basic structural unit of a nucleic acid molecule, consisting of a nitrogen containing base, an pentose sugar (for RNA, said sugar is ribose; for DNA, said sugar is D-deoxyribose) and phosphoric acid. The term “nucleoside” includes molecules that act as precursors of nucleotides but are free of phosphate residues possessed by the nucleotides. By inhibiting the biosynthesis of these molecules or inhibiting the recruitment of these molecules to form nucleic acid molecules, it is possible to inhibit RNA and DNA synthesis; By inhibiting this activity in a manner that targets cancer cells, the ability to divide and replicate tumor cells can be suppressed. There are also nucleotides that act as energy stores (ie, AMPs) or coenzymes (ie, FAD and NAD) rather than forming nucleic acid molecules.

It has been described in various documents that these chemicals can be used for such medical conditions by influencing purine and / or pyrimidine metabolism (see, eg, Christopherson, RI and Lyons, SD (1990) "Potent inhibitors of de novo pyrimidine and purine biosynthesis as chemotherapeutic agents. "See Med. Res. Reviews 10: 505-548). The study of enzymes involved in purine and pyrimidine metabolism has focused on the development of new drugs that can be used, for example, as immunosuppressive or antiproliferative agents (Smith, JL, (1995) "Enzymes in nucleotide synthesis." Curr. Opin.Struct. Biol. 5: 752-757; (1995) Biochem Soc. Transact. 23: 877-902). However, purine and pyrimidine bases, nucleosides and nucleotides are used as intermediates in the biosynthesis of various fine chemicals (e.g., thiamin, S-adenosylmethionine, folate or riboflavin), energy carriers for cells (E.g., ATP or GTP), and chemicals that are commonly used as flavor enhancers (e.g., IMP or GMP) or for various medical uses (e.g., See, Kuninaka, A. (1996) Nucleotides and Related Compounds in Biotechnology Vol. 6, Rehm et al., Editors.VCH: Weinheim, pp. 561-612. In addition, there is an increasing number of studies to develop crop protection chemicals, including fungicides, herbicides and pesticides, by targeting enzymes involved in purine, pyrimidine, nucleoside or nucleotide metabolism.

The metabolism of these compounds in bacteria has been characterized (see, for example, Zalkin, H. and Dixon, JE (1992) "de novo purine nucleotide biosynthesis", in: Progress in Nucleic Acid Research and Molecular Biology , Vol. 42, Academic Press, pp. 259-287 and Michal, G. (1999) "Nucleotides and Nucleosides", Chapter 8 in: Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, Wiley: New York Reference). Purine metabolism is the subject of intensive research and is essential for the normal functioning of cells. Impaired purine metabolism in higher animals can lead to serious diseases such as gout. Purine nucleotides are subjected to ribose-5- in a series of steps through inosine-5'-phosphate (IMP), an intermediate compound that produces guanosine-5'-monophosphate (GMP) or adenosine-5'-monophosphate (AMP). Triphosphates that are synthesized from phosphates and used as nucleotides from these GMPs or AMPs are readily formed. Since these compounds are also used as energy stores, their degradation provides energy for many different biosynthetic processes in the cell. Pyrimidine biosynthesis proceeds by the formation of uridine-5'-monophosphate (UMP) from ribose-5-phosphate. UMP is then converted to cytidine-5'-triphosphate (CTP). The deoxy form of all these nucleotides is produced in a one step reduction reaction in which the diphosphate ribose form of nucleotide is converted to the diphosphate deoxyribose form of nucleotide. After phosphorylation, these molecules may be involved in DNA synthesis.

D. Metabolism and Uses of Trehalose

Trehalose consists of two sugar molecules bound by α, α-1,1 bonds. Trehalose is commonly used in the food industry and the beverage industry as an additive for sweeteners, dried or frozen foods. However, it is also used in the pharmaceutical, cosmetic and biotechnology industries (see, eg, Nishimoto et al., (1998) US Pat. No. 5,759,610, Singer, MA and Lindquist, S. (1998) Trends Biotech. 16 : 460-467, Paiva, CLA and Panek, AD (1996) Biotech. Ann. Rev. 2: 293-314] and Shiosaka, M. (1997) J. Japan 172: 97-102. ). Trehalose is produced by enzymes from a number of microorganisms and naturally released into the surrounding medium, which can be isolated from the medium using methods known in the art.

II. Homeostasis and Environmental Adaptation in Corynebacterium glutamicum

Metabolism and other biochemical processes exist that function in response to environmental conditions such as temperature, pressure, solute concentration and oxygen availability. When one or more such environmental conditions are disturbed or altered to oppose the normal functioning of these cellular processes, the cell must maintain an intracellular environment to perform the cellular process despite hostile extracellular environment. Gram-positive bacteria, such as Corynebacterium glutamicum cells, have a number of mechanisms that allow them to maintain intracellular homeostasis despite adverse extracellular conditions. These include cell walls, proteins that can degrade toxic aromatic and aliphatic compounds, protein hydrolysis mechanisms that can quickly destroy misfolded or misregulated proteins, and intracellular cells that do not occur under conditions that are optimal for bacterial growth. Included are catalysts that allow the reaction to occur.

In addition to simply surviving in a hostile environment, bacterial cells (eg, Corynebacterium glutamicum cells) can also adapt to make use of such conditions. For example, in an environment lacking the desired carbon source, cells may be adapted to grow using less suitable carbon sources. In addition, these cells may utilize less desirable inorganic compounds when commonly used inorganic compounds are not available. Corynebacterium glutamicum cells have a large number of genes adapted to use inorganic and organic molecules, which are not normally used under optimal growth conditions, as nutrients and precursors of metabolism. Further aspects of cellular processes related to homeostasis and adaptation are described further below.

A. Modification and Degradation of Aromatic and Aliphatic Compounds

Bacterial cells are usually exposed to various aromatic and aliphatic compounds in nature. An aromatic compound is an organic molecule which has a cyclic ring structure, and an aliphatic compound is an organic molecule which has an open chain structure rather than a ring structure. Such compounds arise as by-products of industrial processes (eg benzene or toluene), but may also be produced by certain microorganisms (eg alcohols). Many of these compounds are toxic to cells, especially highly reactive aromatic compounds due to their high energy ring structure. Thus, certain bacteria have developed mechanisms that can modify or degrade these compounds so that they are no longer harmful to cells. Cells may contain enzymes that, for example, hydroxylate, isomerize, or methylate aromatic or aliphatic compounds to lower the toxicity of these compounds, or allow modified forms to be processed by the cell's standard disposal and degradation pathways. Will have In addition, the cells will have enzymes that can specifically protect one or more of these potentially harmful substances to protect themselves. Principles and examples of this type of modification and degradation processes in bacteria are described in various literatures (see, eg, Sam, H. (1999) "Procaryotes in Industrial Production" in Lengeler, JW et al. Biology of the Procaryotes, Thieme Verlag: Stuttgart, and Schlegel, HG (1992) Allgemeine Mikrobiologie, Thieme: Stuttgart).

In addition to simply inactivating harmful aromatic or aliphatic compounds, many bacteria have evolved to use these compounds as carbon sources for subsequent metabolism when the desired carbon source of the cell is not available. For example, Pseudomonas (Pseudomonas) strain is known to use toluene, benzene, and 1,10-dichloro-decane as a carbon source (Chang, BV et al (1997 ) Chemosphere 35 (12):. 2807-2815; Wischnak, C et al. (1998) Appl. Environ.Microbiol. 64 (9): 3507-3511; Churchill, SA et al. (1999) Appl. Environ.Microbiol. 65 (2): 549-552. Similar examples from other bacterial species are known in the art.

The ability of certain bacteria to modify or degrade aromatic and aliphatic compounds has begun to be studied. Petroleum is a complex mixture of compounds, including aliphatic molecules and aromatics. For example, by introducing bacteria that can degrade or modify these toxic compounds into the spilled oil, much of the environmental damage can be removed at high efficiency and low cost (Smith, MR (1990) "The biodegradation of aromatic hydrocarbons by bacteria "Biodegradation 1 (2-3): 191-206; and Suyama, T. et al. (1998)" Bacterial isolates degrading aliphatic polycarbonates, "FEMS Microbiol. Lett. 161 (2): 255-261).

B. Metabolism of Inorganic Compounds

Cells (eg bacterial cells) contain large amounts of different molecules such as water, inorganic ions and organics (eg proteins, sugars and other macromolecules). Most of the mass of a typical cell consists of only four atoms: carbon, oxygen, hydrogen and nitrogen. Minerals are included in the cells at low rates, but they are very important for the proper functioning of the cells. Such molecules include phosphorus, sulfur, calcium, magnesium, iron, zinc, manganese, copper, molybdenum, tungsten and cobalt. Many of these compounds are essential for the production of important molecules such as nucleotides (phosphorus) and amino acids (nitrogen and sulfur). Others of these inorganic ions act as cofactors for enzymatic reactions or contribute to osmotic control. All of these molecules must be absorbed by bacteria from the environment.

Each of these inorganic compounds is preferably absorbed by bacteria in a form that is most readily available in standard metabolic devices of the cell. However, bacteria may be in an environment where these preferred forms of compounds are not readily available. In order to survive under such circumstances, it is important for bacteria to have additional biochemical mechanisms that can convert readily available inorganic compound forms with lower metabolic activity to those available for metabolism of cells. Some bacteria have a number of genes that encode enzymes for this purpose and they are expressed only when the desired inorganic compound species is not available. Thus, these genes for the metabolism of various inorganic compounds act as other means that bacteria can use to adapt to suboptimal environmental conditions.

The next most important element in the cell after carbon is nitrogen. Typical bacterial cells contain 12-15% nitrogen. Nitrogen is a component that makes up amino acids, nucleotides, and many other molecules important to cells. Nitrogen may also be used instead of oxygen as the final electron acceptor in energy metabolism. Good sources of nitrogen include ammonia gas or ammonium salts (e.g. NH ₄ Cl, (NH ₄ ) ₂ SO ₄ or NH ₄ OH), nitrates, urea, amino acids, complex nitrogen sources such as corn juice, soy flour, soy protein Organic and inorganic compounds such as yeast extract, meat extract and the like. Ammonium nitrogen is immobilized by the action of certain enzymes, such as glutamate dehydrogenase, glutamine synthase and glutamine-2-oxoglutarate aminotransferase. Amino-nitrogen transfer from one organic molecule to another is performed by aminotransferase, a class of enzymes that transfer one amino group from alpha-amino acid to alpha-keto acid. Nitrate can be reduced through nitrate reductase, nitrite reductase and additional redox enzymes until converted to molecular nitrogen or ammonia, which can be readily utilized by standard metabolic pathways of the cell.

Phosphorus is a compound commonly found in cells in the form of organics and inorganics, and many microorganisms can be absorbed into cells in either inorganic or organic form, although they preferentially absorb inorganic phosphates. The conversion of organic phosphate to a form available to the cell is necessary for the action of phosphatase (eg, phytase that hydrolyzes phytate-producing phosphate and inositol derivatives). Phosphates are an important factor in the synthesis of nucleic acids and also play an important role in energy metabolism of cells (eg, the synthesis of ATP, ADP and AMP).

Sulfur is required for the synthesis of amino acids (eg methionine and cysteine), vitamins (eg thiamine, biotin and lipoic acid) and iron / sulfur proteins. Although thiosulfate, sulfite and sulfides are commonly used, bacteria primarily obtain sulfur from inorganic sulfates. Under conditions where these compounds are not readily available, many bacteria express genes that make use of sulfonate compounds such as 2-aminosulfonate (taurine) (Kertesz, MA (1993) "Proteins induced by sulfate limitation in Escherichia coli , Pseudomonas putida , or Staphylococcus aureus . "J. Bacteriol. 175: 1187-1190).

Other inorganic atoms, such as metal or calcium ions, are also essential for cell survival. Iron, for example, plays an important role in redox reactions and is a cofactor of iron / sulfur proteins, heme proteins and cytochromes. Bacterial cells can take up iron by the chelating agent siderophore, which binds to and moves extracellular iron ions into the cell. Reference may be made to the following references for metabolism of iron and other inorganic compounds: Lengeler et al. (1999) Biology of Prokaryotes, Thieme Verlag: Stuttgart; Neidhardt, FC et al., Editors. Escherichia coli and Salmonella . ASM Press: Washington, DC; Sonenshein, AL et al., Editors. (1996) Bacillus subtilis and Other Gram-Positive Bacteria, ASM Press: Washington, DC; Voet, D. and Voet, JG (1992) Biochemie, VCH: Weinheim; Brock, TD and Madigan, MT (1991) Biology of Microorgansisms, 6 ^th ed. Prentice Hall: Englewood Cliffs, pp. 267-269; Rhodes, PM and Stanbury, PF Applied Microbial Physiology-A Practical Approach, Oxford Univ. Press: Oxford.

C. Enzymes and Protein Hydrolysis

Intracellular conditions in which bacteria such as Corynebacterium glutamicum are optimized may not be the conditions under which many biochemical reactions normally occur. In order for the reaction to proceed under physiological conditions, the cells use enzymes. Enzymes are biological protein catalysts that provide specific environments to spatially induce the reaction of molecules or to lower the energy barrier to biochemical reactions. Different enzymes catalyze different reactions, and each enzyme can be transcribed, translated or post-translated so that the reaction occurs only at specific times under appropriate conditions. Enzymes contribute to the degradation (eg proteases), synthesis (eg synthase) or modification (eg transferase or isomerase) of a compound, all of which produce the required compounds in cells To make it possible. In other words, enzymes contribute to maintaining homeostasis of cells.

However, the fact that the enzyme is optimized for activity under the most viable physiological conditions of bacteria means that if the environmental conditions are disturbed, the enzyme activity is also likely to be disturbed. For example, a change in temperature can produce a strangely folded protein, and a change in pH as well-since protein folding depends primarily on the electrostatic and hydrophobic interactions of amino acids in the polypeptide chain, the charge on the individual amino acids Changes (which may be caused by changes in the pH of the cell) can have a significant impact on the ability of the protein to fold correctly. The change in temperature effectively changes the amount of reaction energy possessed by the polypeptide molecule, which affects the ability of the polypeptide to be correctly folded and framed into a very stable structure. Misfolded proteins are harmful to cells for two reasons. First, strangely folded proteins may have unusual activity or no activity at all. Second, misfolded proteins lack the structural regions essential for proper regulation by other cell lines, allowing them to remain uncontrolled and continue to be active.

Cells have a mechanism (protein hydrolysis) that destroys cells quickly before they appear damaged by misfolded enzymes and regulatory proteins (protein hydrolysis). Proteins such as la / lon and Clp proteins specifically recognize and degrade misfolded proteins (Sherman, MY, Goldberg, AL (1999) EXS 77: 57-78 and references therein; Porankiewicz J. (1999) Molec. Microbiol. 32 (3): 449-58 and references therein: Neidhardt, FC, et al. (1996) E. coli and Salmonella , ASM Press: Washington, DC and references therein; And Pritchard, GG, and Coolbear, T. (1993) FEMS Microbiol. Rev. 12 (1-3): 179-206 and references therein). These enzymes bind to proteins that are misfolded or unfolded and degrade them in an ATP-dependent manner. Thus, proteolysis acts as an important mechanism used by cells to prevent damage to normal cell function upon environmental changes, and proteolysis is also toxic due to misregulated and / or abnormal enzymes or regulatory activity. This allows the cells to survive under conditions and environments that can be.

In addition, proteolysis shows an important function in cells under optimal environmental conditions. In normal metabolic processes, proteases aid in the hydrolysis of peptide bonds, through catabolism of complex molecules, to provide the necessary degradation products, and assist in protein modification. Secretory proteases play an important role in the catabolism of external nutrients even before these compounds enter the cell. In addition, proteolytic activity may itself function as a regulator. Spore formation in Bacillus subtilis and cell cycle progression in Caulobacter spp. Are known to be regulated by significant proteolytic events in each species (Gottesman, S. (1999). ) Curr. Opin.Microbiol. 2 (2): 142-147). Thus, proteolytic processes are secondary to the survival of cells under optimally and optimally environmental conditions and contribute to maintaining overall homeostasis in the cells.

D. Cell Wall Generation and Rearrangement

The biochemical devices of cells make it easy to adapt to other and potentially adverse environments, while cells require a universal mechanism that allows them to protect themselves from the environment. In many bacteria, cell walls provide such protection and also play an important role in adhesion, cell growth and division, and the transport of desired solutes and wastes.

For this function, cells require substantially higher concentrations of metabolites and other molecules within the cell than in the surrounding medium. Because these metabolites are primarily intracellular due to hydrophobic cell membranes, the trend of the system is to allow water molecules to enter the cells from the external medium so that the internal solute concentrations match the external concentrations. Osmotic lysis of cells can occur because water molecules can easily travel to and from the cell membrane and the cell membrane cannot withstand the resulting swelling and pressure. The firmness of the cell wall significantly improves the cell's ability to resist this pressure and provides additional barriers to the unwanted diffusion of metabolites and desired solutes from the cell. Similarly, cell walls also prevent unwanted substances from entering the cells.

In addition, cell walls are involved in many cellular processes such as adhesion, cell growth, and cell division. Because the cell wall completely surrounds the cell, all interactions of the cell with the surrounding environment must be mediated by the cell wall. Thus, the cell wall must be involved in all processes in which cells attach to other cells and to the desired surface. In addition, cells cannot grow or divide unless accompanied by changes in the cell wall. Since the protection provided by the cell wall requires its presence in growth, morphogenesis and replication, one of the important stages of cell division is the synthesis of the cell wall within the cell so that new cells can divide from existing cells. Thus, cell wall biosynthesis is regulated with cell growth and cell division (Sonenshein, AL et al., Editors. (1993) Bacillus subtilis and Other Gram-Positive Bacteria, ASM: Washington, DC).

The structure of the cell wall differs between Gram-positive and Gram-negative bacteria. In these two types, however, the basic structural units of the cell wall are similar: the two polysaccharides, N-acetyl glucosamine (NAG) and N-acetyl myramine acid (NAM), are amino acids (mainly L-alanine, D-glutamate, dia). Superposition lattice crosslinked by minopimelic acid and D-alanine) (called "peptidoglycan"). Processes associated with cell wall synthesis are known (Michal, G., ed. (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, Wiley: New York).

In Gram-negative bacteria, the lining is covered with a single layer of peptidoglycan called Murein-Saculus (thickness about 10 nm). This peptidoglycan structure is very strong and the shape of the organism is determined by the structure. The outer sheath of the murine-saculus is covered with an outer membrane containing porin and other membrane proteins, phospholipids and lipopolysaccharides. To maintain strong binding to the outer membrane, Gram-negative cell walls are interspersed with lipid molecules that attach themselves to the surrounding membrane.

In bacteria of Gram-positive bacteria, such as Corynebacterium glutamicum, the cell membrane is covered with multilayered peptidoglycans with a thickness of 20 to 80 nm (Lengeler et al. (1999) Biology of Prokaryotes Thieme Verlag: Stuttgart, pp. 913-918, pp. 875-899 and pp. 88-109, and references therein). Gram positive cell walls also contain teicosane, glycerol polymers, or ribitols linked through phosphate groups. Teicoic acid can also bind amino acids and form covalent bonds with muraminic acid. In addition, lipoteichoic acid and teicuronic acid may be present in the cell wall. If they are present, cell surface structures such as flagella or capsules will attach to the cell wall layer.

III. Elements and Methods of the Invention

The present invention relates, at least in part, to new molecules, referred to herein as HA nucleic acid and protein molecules, which function to participate in the maintenance of homeostasis of Corynebacterium glutamicum or related to adaptation of this microorganism to other environmental conditions. Based on discovery. In one embodiment, the HA molecule is involved in cell wall biosynthesis or rearrangement of Corynebacterium glutamicum, metabolism of inorganic compounds, modification or degradation of aromatic or aliphatic compounds, or have enzymatic activity or proteolytic activity. In a preferred embodiment, the activity of the HA molecules of the present invention on cell wall biosynthesis or rearrangement of Corynebacterium glutamicum, metabolism of inorganic compounds, modification or degradation of aromatic or aliphatic compounds, or enzymatic activity or proteolytic activity Corynebacterium glutamicum has an important effect on the production of the desired fine chemicals. In a particularly preferred embodiment, the cellular process of Corynebacterium glutamicum involving HA molecules (e.g., cell wall biosynthesis or rearrangement of Corynebacterium glutamicum, metabolism of inorganic compounds, of aromatic or aliphatic compounds) By altering the activity of the HA molecules of the present invention such that the activity on modified or degraded, or enzymatic or proteolytic activity) is altered, yield, productivity and / or production of fine chemicals by Corynebacterium glutamicum Efficiency changes directly or indirectly.

The term "HA protein" or "HA polypeptide" includes proteins involved in many cellular processes associated with homeostasis of Corynebacterium glutamicum or the ability of Corynebacterium glutamicum cells to adapt to adverse environmental conditions. . For example, the HA protein may be cell wall biosynthesis or rearrangement of Corynebacterium glutamicum, inorganic compound metabolism of Corynebacterium glutamicum, modification of aromatic or aliphatic compounds at Corynebacterium glutamicum or Is involved in degradation or has the enzymatic or proteolytic activity of Corynebacterium glutamicum. Examples of HA proteins include proteins encoded by the HA genes described in Table 1 and Appendix A. The term "HA gene" or "HA nucleic acid sequence" includes nucleic acid sequences encoding HA proteins, consisting of coding regions and corresponding 5 'and 3' untranslated sequence regions. Examples of HA genes include those listed in Table 1. The term “production” or “productivity” is a term recognized in the art and includes the concentration of fermentation product produced (eg, kg product per liter per hour) at a given time and within a given fermentation volume. . The term “efficiency of production” includes the time required to achieve a specific product concentration (eg, the time it takes for the cell to obtain a specific proportion of the fine chemical product). The term "yield" or "product / carbon yield" is a term recognized in the art and includes the conversion efficiency of a carbon source into a product (ie, a fine chemical). This term is generally denoted, for example, in kg of product per kg of carbon source. By increasing the yield or yield of the compound, the amount of recovered molecules or useful molecules of the compound in the obtained amount of culture obtained for a given time is increased. The term "biosynthesis" or "biosynthetic pathway" is a term recognized in the art and involves the synthesis of compounds, preferably organic compounds, from intermediate compounds by cells, and can be a multistep highly regulated process. The term "degradation" or "degradation pathway" is a term recognized in the art and includes the degradation by a cell into a degradation product of a compound, preferably an organic compound (generally, a molecule of smaller or less complex structure) and This can be a multi-step, highly controlled process. Metabolism of certain compounds (eg, amino acid metabolism such as glycine) includes the overall biosynthesis, modification, and degradation pathways of cells associated with such compounds. The term "always" is a term recognized in the art and encompasses all mechanisms that cells use to maintain a constant intracellular environment despite prevailing extracellular environmental conditions. Non-limiting examples of such processes include the use of cell walls to prevent osmotic lysis due to high solute concentrations within the cell. The term "adaptation" or "adaptation to environmental conditions" is a term recognized in the art, where adverse environmental conditions (generally speaking, environmental conditions without desirable nutrients, or environmental conditions such as pH, osmotic concentration, oxygen ratio, etc. It includes all the mechanisms used by the cell so that it can survive under the optimal survival range. Many cells, including Corynebacterium glutamicum, have genes that encode proteins that are expressed under such environmental conditions, allowing them to continue to grow in secondary optimal conditions.

In another embodiment, the HA molecules of the invention can modulate the production of the desired molecule, such as a fine chemical, in a microorganism such as Corynebacterium glutamicum. There are a number of mechanisms in which the modification of HA molecules of the present invention can directly affect the yield, productivity and / or efficiency of fine chemical production from Corynebacterium glutamicum strains incorporating modified proteins. For example, by manipulating the enzymes to increase or decrease the activity or number of enzymes that modify or degrade aromatic or aliphatic compounds, the production of one or more microchemicals that are modifications or degradation products of these compounds can be controlled. Similarly, enzymes involved in metabolism of inorganic compounds provide molecules important for biosynthesis of fine chemicals such as amino acids, vitamins and nucleic acids (eg, phosphorus, sulfur and nitrogen molecules). By varying the activity or number of these enzymes in Corynebacterium glutamicum, the conversion of these inorganic compounds can be increased (or using alternative inorganic compounds) to improve the incorporation of inorganic atoms into the fine chemicals. When genetically engineering the enzymes of Corynebacterium glutamicum associated with universal cellular processes, many of these enzymes directly modify fine chemicals (eg, amino acids) or synthesize or secrete fine chemicals. By modifying the enzymes involved, the productivity of fine chemicals can be directly improved. In addition, modulating the activity or number of cellular proteases can directly affect the productivity of fine chemicals because many proteases can degrade microchemicals or enzymes involved in their production or disruption. . The enzymes mentioned above as involved in the modification or degradation of aromatic or aliphatic compounds, universal biocatalysis, inorganic compound metabolism, or protein hydrolysis are themselves fine chemicals, and their activities in various test and industrial fields. This is necessary. From large-scale cultures of Corynebacterium glutamicum or related bacteria by changing the copy number of the genes for one or more of these enzymes in Corynebacterium glutamicum to increase the number of proteins produced by the cells. It can increase the potential yield or production efficiency of the protein.

In addition, changes in the HA protein of the present invention may indirectly affect the yield, productivity, and / or efficiency of fine chemical production from Corynebacterium glutamicum incorporating the altered protein. For example, by regulating the activity and / or number of proteins involved in the construction or rearrangement of the cell wall, the structure of the cell wall itself can be altered to make the cell more resistant to mechanical and other stresses present in large-scale fermentation cultures. have. In addition, large-scale growth of Corynebacterium glutamicum requires significant cell wall production. Controlling the activity or number of cell wall biosynthesis or degrading enzymes can speed up cell wall biosynthesis, which increases the growth rate in the culture of the microorganisms, thereby increasing the number of cells producing the desired fine chemicals.

By modifying the HA enzyme of the present invention, the yield, productivity or production efficiency of producing one or more microchemicals from Corynebacterium glutamicum can be affected. For example, many universal enzymes of Corynebacterium glutamicum can have a strong effect on the overall cellular processes (eg, regulatory processes), which have a significant effect on the metabolism of fine chemicals.

Thus, proteases (ie, enzymes that modify or degrade aromatic or aliphatic compounds that may be toxic) serve to increase the viability of Corynebacterium glutamicum. This protease aids in the selective removal of misfolded or misregulated proteins, for example, proteins that may arise under relatively stressful environmental conditions seen in large fermentation cultures. By changing these proteins, it is possible to further increase the activity of the protein and to increase the viability of Corynebacterium glutamicum in culture. Proteins for the modification or degradation of aromatic / aliphatic compounds not only eliminate the toxicity of these waste compounds (either as contaminants in the culture medium or as waste from the cells themselves), but also when the optimal carbon source in the culture is limited. It allows cells to use alternative carbon sources. By increasing their number and / or activity, it is possible to increase the survival rate of Corynebacterium glutamicum in culture. The inorganic compound metabolic protein of the present invention is essential for the overall viability of the cell, in particular because it supplies the cell with the inorganic molecules necessary for the synthesis of all proteins and nucleotides. Increasing the number of viable cells producing one or more of the desired fine chemicals in a large scale culture will in turn increase the yield, productivity and / or efficiency of the fine chemical production in the culture.

A suitable starting point for preparing nucleic acid sequences of the present invention is the genome of the Corynebacterium glutamicum strain, which can be distributed in the American Type Culture Collection with ATCC 13032.

Nucleic acid sequences of the invention can be prepared from these nucleic acid sequences by modifications described in Table 1 using conventional methods.

Various aspects of the invention are described in more detail in the following subsections.

A. Isolated Nucleic Acid Molecules

One aspect of the invention provides for use as hybridization probes or primers for identification or amplification of HA-encoding nucleic acids (eg, HA DNA) as well as isolated nucleic acid molecules encoding HA polypeptides or biologically active portions thereof. Enough nucleic acid fragments. The term “nucleic acid molecule” is meant to include DNA or RNA analogs generated using DNA molecules (eg cDNA or genomic DNA), RNA molecules (eg mRNA), and nucleotide analogues. The term also includes untranslated sequences located at both the 3 'and 5' ends of the coding region of the gene, at least about 100 nucleotide sequences upstream from the 5 'end of the coding region, and 3' of the coding region of the gene. At least about 20 nucleotide sequences downstream from the terminus. The nucleic acid molecule may be single stranded or double stranded, preferably double stranded DNA. An “isolated” nucleic acid molecule is one that is separated from other nucleic acid molecules present in a natural source of nucleic acid. Preferably, an “isolated” nucleic acid does not have a sequence flanking the native nucleic acid (ie, sequences located at the 5 ′ and 3 ′ ends of the nucleic acid) in the genomic DNA of the organism from which it is derived. For example, in various embodiments, an isolated HA nucleic acid molecule is about 5 kb flanking the native nucleic acid molecule in the genomic DNA of the cell from which it is derived (eg, Corynebacterium glutamicum cells), Nucleotide sequences of size less than 4 kb, 3 kb, 2 kb, 1 kb, 0.5 kb or 0.1 kb. In addition, an “isolated” nucleic acid molecule, such as a DNA molecule, is substantially free of other cellular material or culture medium when produced by recombinant technology, or chemical precursors or other chemicals when chemically synthesized.

Standard molecular biology techniques and the sequence information provided herein can be used to isolate nucleic acid molecules of the invention, such as nucleic acid molecules having the nucleotide sequence of Appendix A or portions thereof. For example, complete sequences from Appendix A or portions thereof may be used as hybridization probes and standard hybridization techniques (e.g., Sambrook, J., Fritsh, EF, and Maniatis T. Molecular Cloning: A Laboratory Manual. , ed., a hybridization technique as described in the Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989) from Corynebacterium glutamicum banks. Tamicum HA DNA can be isolated. Moreover, nucleic acid molecules comprising the complete sequence or portion thereof from Appendix A can be isolated via polymerase chain reaction using oligonucleotide primers generated based on the sequence (eg, in Appendix A It is also possible to isolate nucleic acid molecules comprising the complete sequence or portions thereof from Appendix A via polymerase chain reaction using oligonucleotide primers generated based on the same sequence as the sequence). For example, mRNA can be isolated from normal endothelial cells (eg, by the guanidinium thiocyanate extraction method of Chilgwin et al. (1979) Biochemistry 18: 5294-5299) and cDNA Molone MLV reverse transcriptase available from reverse transcriptase (eg Gibco / BRL (Bethesda, MD); or from Seikagaku America, Inc., St. Petersburg, FL) Possible AMV reverse transcriptase). Synthetic oligonucleotide primers for polymerase chain reaction amplification can be prepared based on any of the nucleotide sequences described in Appendix A. Nucleic acids of the invention can be amplified using cDNA or, alternatively, genomic DNA as a template and using appropriate oligonucleotide primers according to standard PCR amplification techniques. The nucleic acid thus amplified can be cloned into an appropriate vector and characterized by DNA sequencing. In addition, oligonucleotides corresponding to HA nucleotide sequences can be prepared using standard synthetic techniques, such as automated DNA synthesizers.

In a preferred embodiment, the isolated nucleic acid molecules of the invention comprise one of the nucleotide sequences set forth in Appendix A. The sequence of Appendix A corresponds to the Corynebacterium glutamicum HA cDNA of the present invention. This cDNA includes the 5 'untranslated and 3' untranslated sequences shown in Appendix A, as well as the sequence of the HA protein (ie, the "coding region" shown in each sequence in Appendix A). In addition, the nucleic acid molecule may comprise only the coding region of any of the sequences of Appendix A.

In another preferred embodiment, an isolated nucleic acid molecule of the invention comprises a nucleic acid molecule that is complementary to any nucleotide sequence or portion thereof described in Appendix A, wherein the nucleic acid molecule is sufficiently complementary to any nucleotide sequence described in Appendix A. And hybridize with any sequence described in Appendix A to form a stable double chain.

In another preferred embodiment, the isolated nucleic acid molecule of the present invention is at least about 50% to 60%, preferably at least about 60% to 70%, more preferably about 70 with the nucleotide sequence or portion thereof described in Appendix A. % To 80%, 80% to 90% or 90% to 95% or more, even more preferably about 95%, 96%, 97%, 98% or 99% or more homologous nucleotide sequences. In a further preferred embodiment, an isolated nucleic acid molecule of the invention comprises a nucleotide sequence that hybridizes with any nucleotide sequence or portion thereof described in Appendix A, for example under stringent conditions.

In addition, the nucleic acid molecules of the present invention may comprise only a portion of the coding region of any sequence described in Appendix A, for example a fragment that can be used as a probe or primer or a fragment encoding a biologically active portion of an HA protein. Identify and / or clone HA homologs from other Corynebacteria or related species as well as HA homologs in other types of cells and organisms using nucleotide sequences determined from cloning HA genes from Corynebacterium glutamicum. Probes and primers designed for use can be generated. Probes or primers usually comprise substantially purified oligonucleotides. Oligonucleotides are typically about 12, preferably about 25, more preferably the sense strand of any sequence described in Appendix A, the antisense strand of any sequence described in Appendix A, or their naturally occurring variants under stringent conditions Comprises a nucleotide sequence region that hybridizes with about 40, 50, or 75 or more consecutive nucleotides. Primers based on the nucleotide sequence of Appendix A can be used in a PCR reaction to clone an HA homologue. Probes based on HA nucleotide sequences can be used to detect transcript or genomic sequences encoding proteins that are identical or homologous. In a preferred embodiment, the probe further comprises a labeling group attached thereto, wherein the labeling group can be, for example, a radioisotope, a fluorescent compound, an enzyme or a cofactor of an enzyme. Such probes are cells that misexpress HA proteins, for example by measuring the amount of HA-encoding nucleic acid in a cell sample, for example by detecting HA mRNA levels or determining whether genomic HA genes are mutated or deleted. It can be used as part of a diagnostic assay kit to confirm.

In one embodiment, a nucleic acid molecule of the invention encodes a protein or portion thereof comprising an amino acid sequence that is sufficiently homologous to the amino acid sequence of Appendix B, which protein or portion thereof is in Corynebacterium glutamicum It maintains the ability to participate in maintenance of homeostasis or to perform functions associated with adaptation to other environmental conditions. As used herein, the term “sufficiently homologous” includes a minimum number of amino acid residues that are the same or equivalent to the amino acid sequence of Appendix B (eg, amino acid residues having side chains similar to amino acid residues in any sequence of Appendix B). A protein or portion thereof having an amino acid sequence, which may perform a function involved in maintaining homeostasis in Corynebacterium glutamicum or related to adaptation of this microorganism to other environmental conditions. Cell wall biosynthesis or rearrangement of Corynebacterium glutamicum, metabolism of inorganic compounds, modification or degradation of aromatic or aliphatic compounds, or having enzymatic activity or proteolytic activity of Corynebacterium glutamicum Proteins may act in the production and secretion of one or more microchemicals as described herein. Examples of such activities are also described herein. Thus, "function of HA protein" directly or indirectly contributes to the yield, productivity and / or efficiency of production of one or more fine chemicals. Examples of HA protein activity are listed in Table 1.

In another embodiment, the protein comprises at least about 50% to 60%, preferably at least about 60% to 70%, more preferably about 70% to 80%, 80% to 90% of the complete amino acid sequence of Appendix B , 90% to 95% or more, most preferably about 96%, 97%, 98% or 99% or more homology.

The portion of the protein encoded by the HA nucleic acid molecule of the invention is preferably the biologically active portion of one of the HA proteins. As used herein, the term "biologically active portion of an HA protein" refers to the maintenance of the starburst of Corynebacterium glutamicum, to perform a function associated with adaptation of this microorganism to other environmental conditions, or Table 1 A portion of an HA protein having activity as described, eg, domains / motifs thereof. HA protein or a biologically active portion thereof is involved in cell wall biosynthesis or rearrangement of Corynebacterium glutamicum, metabolism of inorganic compounds, modification or degradation of aromatic or aliphatic compounds, or of Corynebacterium glutamicum To determine whether there is enzyme activity or proteolytic activity, an assay of enzyme activity can be performed. Such assay methods are well known to those skilled in the art, as described in Example 8.

Additional nucleic acid fragments encoding biologically active portions of the HA protein isolate portions of any amino acid sequence of Appendix B that express the coding portion of the HA protein or HA peptide (eg, by recombinant expression in vitro) and It can be prepared by measuring the activity of the encoded portion of the HA protein or peptide.

Moreover, the present invention includes nucleic acid molecules (and portions thereof) that differ from any of the nucleotide sequences set forth in Appendix A, but which encode the same HA protein as those encoded by the nucleotide sequences of Appendix A due to the degeneracy of the genetic code. do. In another embodiment, an isolated nucleic acid molecule of the invention has a nucleotide sequence encoding a protein having the amino acid sequence shown in Appendix B. In another embodiment, the nucleic acid molecules of the invention encode a full-length Corynebacterium glutamicum protein that is essentially homologous to the amino acid sequence of Appendix B (encoded by the open reading frame shown in Appendix A).

An isolated protein encoding an HA protein homologous to the protein sequence of Appendix B by introducing a substitution, addition or deletion of one or more nucleotides within the nucleotide sequence of Appendix A such that a substitution, addition or deletion of one or more amino acids in the protein to be encoded is introduced. Nucleic acid molecules can be produced. Mutations can be introduced into any nucleotide sequence of Appendix A by standard techniques such as site-directed mutagenesis and PCR-mediated mutagenesis. Preferably, conserved amino acid substitutions can be made at one or more non-essential amino acid residues contemplated. "Conserved amino acid substitutions" refer to replacement of amino acid residues with amino acid residues having similar side chains. Groups of amino acid residues with similar side chains are defined in the art. These groups include amino acids with basic side chains (eg lysine, arginine, histidine), amino acids with acidic side chains (eg aspartic acid, glutamic acid), amino acids with uncharged polar side chains (eg, Glycine, asparagine, glutamine, serine, threonine, tyrosine, cysteine), amino acids with nonpolar side chains (e.g. alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan), beta-branched side chains Amino acids having (eg threonine, valine, isoleucine) and amino acids having aromatic side chains (eg tyrosine, phenylalanine, tryptophan, histidine). Thus, the non-essential amino acid residues expected in the HA protein are preferably substituted with other amino acid residues from the same side chain group. Alternatively, in another embodiment, the mutations can be introduced randomly along all or a portion of the HA coding sequence, for example by saturation mutagenesis, to identify variants that possess HA activity. Variants can be screened for HA activity described herein. Any of the sequences of Appendix A can be mutagenesis, followed by recombinant expression of the encoded protein and, for example, the activity of the protein can be determined using the assays described herein (see Example 8).

B. Recombinant Expression Vectors and Host Cells

Another aspect of the invention relates to an expression vector containing a nucleic acid encoding a vector, preferably a HA protein (or a portion thereof). As used herein, the term "vector" refers to a nucleic acid capable of transporting other nucleic acids linked thereto. In one type of vector, "plasmid" means a circular double-stranded DNA ring into which additional DNA fragments can be ligated. Another type of vector, a viral vector, can ligate additional DNA fragments into the viral genome. Certain vectors have the ability to autonomously replicate within the introduced host cell (eg, bacterial vectors with bacterial replication initiation points and episomal vectors in mammals). Other vectors (eg, mammalian non-episomal vectors) are inserted into the host genome upon introduction into the host cell and thereby replicate with the host genome. In addition, certain vectors can induce the expression of functionally linked genes. Such vectors are referred to herein as "expression vectors." In general, expression vectors used in recombinant DNA techniques are usually in the form of plasmids. Since plasmids are the most commonly used vector form, "plasmid" and "vector" can be used interchangeably herein. The invention also includes other forms of expression vectors such as viral vectors that provide equivalent functions (eg, replication defective retroviruses, adenoviruses and adeno-associated viruses).

The recombinant expression vector of the present invention comprises the nucleic acid of the present invention in a form suitable for expression of the nucleic acid in a host cell, and the suitable form means that the recombinant expression vector is one or more regulatory sequences selected based on the host cell for expression (the nucleic acid to be expressed). Functionally linked to a sequence). In a recombinant expression vector, "functionally linked" means linked to the regulatory sequence (s) such that the desired nucleotide sequence is capable of expression (e.g., in an in vitro transcription / translation system or when the vector is introduced into a host cell. Intracellular). The term "regulatory sequence" is intended to include promoters, enhancers and other expression control elements (eg, polyadenylation signals). Such regulatory sequences are described, for example, in Goeddel, Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, CA (1990). Regulatory sequences include constitutive expression of nucleotide sequences within multiple host cell types and expression of nucleotide sequences only within specific host cells. Those skilled in the art will appreciate that expression vector design may vary depending on factors such as the choice of host cell to be transformed, the degree of expression of the protein of interest, and the like. The expression vector of the present invention can be introduced into a host cell to produce a protein or peptide, including a fusion protein or peptide encoded by the nucleic acids described herein (eg, HA proteins, mutated forms of HA proteins, fusion proteins, etc.). .

The recombinant expression vectors of the invention can be designed for HA protein expression in prokaryotic or eukaryotic cells. For example, the HA gene can include bacterial cells such as Corynebacterium glutamicum, insect cells (using baculovirus expression vectors), yeast and other fungal cells (Romanos, MA et al . (1992) "Foreign gene expression in yeast: a review ", Yeast 8: 423-488; van den Hondel, CAMJJ et al . (1991)" Heterologous gene expression in filamentous fungi "in: More Gene Manipulations in Fungi, JW Bennet & LL Lasure, Editors. , pp. 396-428: Academic Press: San Diego; and van den Hondel, CAMJJ & Punt, PJ (1991) "Gene transfer systems and vector development for filamentous fungi, in: Applied Molecular Genetics of Fungi, Peberdy, JF et al ., Editors., Pp. 1-28, Cambridge University Press: Cambridge], protozoan cells and multicellular plant cells (Schmidt, R. and Willmitzer, L. (1988) High efficiency Agrobacterium tumefactiens -mediated transformation of Arabidopsis thaliana leaf and cotyledon explants "Plant Cell Rep:. 583-586] reference) or mammals Four can be expressed within. Suitable host cells are further described in Goeddel, Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, CA (1990). Alternatively, recombinant expression vectors can be transcribed and translated in vitro using, for example, a T7 promoter regulatory sequence and a T7 polymerase.

Expression of proteins in prokaryotic cells is generally performed with constitutive or inducible promoter containing vectors that allow for the expression of fusion or non-fusion proteins. Fusion vectors add a number of amino acids to their encoded protein, generally at the amino terminus of the recombinant protein. Such fusion vectors are typically for three purposes: 1) to increase expression of the recombinant protein, 2) to increase the solubility of the recombinant protein, and 3) to facilitate the purification of the recombinant protein by acting as a ligand in affinity purification. Usually, in the fusion expression vector, a protein cleavage site is introduced at the junction of the fusion residue and the recombinant protein, so that it is possible to separate the recombinant protein from the fusion residue after purifying the fusion protein. Such enzymes and their corresponding recognition sequences include factor Xa, thrombin and enterokinase.

Typical fusion expression vectors include pGEX (Pharmacia Biotech Inc; see Smith, DB and Johnson, KS (1988) Gene 67: 31-40), pMAL (New England Biolabs, Beverly, Mass.) And pRIT5 (Pharmacia, Piscataway, NJ), which fuses glutathione-S-transferase (GST), maltose E binding protein or protein A, respectively, to the target recombinant protein. In one embodiment, the coding sequence of the HA protein was cloned into a pGEX expression vector to generate a fusion protein coding vector containing a GST-thrombin cleavage site-X protein at the N-terminus to C-terminus. The fusion protein can be purified by affinity chromatography using glutathione-agarose resin. Recombinant HA proteins that are not fused to GST can be obtained by cleaving the fusion protein with thrombin.

Examples of suitable inducible non-fusion E. coli expression vectors are pTrc (see Amann et al ., (1988) Gene 69: 301-315) and pET 11d (Studier et al ., Gene) . Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, California (1990) 60-89). The expression of the target gene from pTrc is due to the RNA polymerase transcription of the host via a hybrid trp-lac fusion promoter. The expression of the target gene from the pET 11d vector is by transcription mediated by co-expressed viral RNA polymerase (T7 gn1) via the T7-gn10-lac fusion promoter. This viral polymerase is supplied by host strain BL21 (DE3) or HMS174 (DE3) from a supernatant lambda probe containing the T7 gn1 gene under the transcriptional control of the lacUV 5 promoter.

As part of a strategy for maximizing expression of recombinant proteins, the expression of these proteins in host bacteria has reduced the ability to cleave the recombinant protein by proteolysis (Gottesman, S., Gene Expression Technology: Methods in Enzymology). 185, Academic Press, San Diego, California (1990) 119-128). Another strategy is to modify the nucleic acid sequence of the nucleic acid inserted into the expression vector so that the individual codons for each amino acid are the preferred codons for the bacteria selected for expression (eg, Corynebacterium glutamicum). (See Wada et al . (1992) Nucleic Acids Res . 20: 2111-2118). Such modification of nucleic acid sequences of the invention can be performed by standard DNA synthesis techniques.

In other embodiments, the HA protein expression vector is a yeast expression vector. Examples of expression vectors of yeast Saccharomyces cerevisiae include pYepSec1 (Baldari, et al ., (1987) Embo J. 6: 229-234), pMFa (Kurjan and Herskowitz, ( 1982) Cell 30: 933-943), pJRY88 (see Schultz et al ., (1987) Gene 54: 113-123) and pYES2 (Invitrogen Corporation, San Diego, Calif.). Suitable vectors and methods for making them for other fungi, such as filamentous fungi, are described by van den Hondel, CAMJJ & Punt, PJ (1991) "Gene transfer systems and vector development for filamentous fungi, Applied Molecular Genetics of Fungi, JF Peberdy. , et al ., Editors., pp. 1-28, Cambridge University Press: Cambridge.

Alternatively, baculovirus expression vectors can be used to express the HA proteins of the invention in insect cells. Baculovirus vectors for protein expression in cultured insect cells (e.g., Sf 9 cells) include pAc (see Smith et al . (1983) Mol. Cell Biol . 3: 2156-2165) and pVL systems ( Lucklow and Summers (1989) Virology 170: 31-39).

In other embodiments, the HA protein of the invention can be expressed in plant cells from single cell plant cells (eg, protozoa) or higher plants (eg, seed plants such as crop plants). Examples of plant expression vectors are described in Bekker, D., Kemper, E., Schell, J. and Masterson, R. (1992) "New plant binary vectors with selectable markers located proximal to the left border", Plant Mol. Biol . 20: 1195-1197, and Bevan, MW (1984) “Binary Agrobacterium vectors for plant transformation”, Nucl. Acid. Res . 12: 8711-8721.

In another embodiment, a mammalian expression vector is used to express the nucleic acid of the invention in a mammalian cell. Examples of mammalian expression vectors include pCDM8 (see Seed, B. (1987) Nature 329: 840) and pMT2PC (see Kaufman et al . (1987) EMBO J. 6: 187-195). . When using mammalian cells, the regulatory function of the expression vector is usually by viral regulatory elements. For example, commonly used promoters are derived from polyoma, adenovirus 2, cytomegalovirus and simian virus 40. Other expression systems suitable for both prokaryotic and eukaryotic cells can be found in Chapters 16 and 17 of Sambrook, J., Fritsh, EF, and Maniatis, T. Molecular Cloning: A Laboratory Manual. 2nd. ed. Cold Spring Harbor Laboratory , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989.

In other embodiments, recombinant mammalian expression vectors allow for differential nucleic acid expression in certain cell types (eg, tissue-specific regulatory elements are used to express nucleic acids). Tissue-specific regulatory elements are known in the art. Non-limiting examples of suitable tissue-specific promoters include albumin promoters (liver-specific; see Pinkert et al . (1987) Genes Dev . 1: 268-277), lymphoid-specific promoters (see literature). (See Calame and Eaton (1988) Adv. Immunol . 43: 235-275), in particular promoters of T cell receptors (see Winoto and Baltimore (1989) EMBO J. 8: 729-733) and immunoglobulins Promoters (see Banerji et al . (1983) Cell 33: 729-740, Queen and Baltimore (1983) Cell 33: 741-748), neuron-specific promoters (eg neurofilament promoters); See Byrne and Ruddle (1989) PNAS 86: 5473-5477), pancreas-specific promoters (see Edlund et al . (1985) Science 230: 912-916) and mammary gland-specific Promoters (eg, whey promoter; see US Pat. No. 4,873,316 and European Application No. 264,166). In addition, developmental-regulatory promoters, such as the hox promoter in mice (see Kessel and Gruss (1990) Science 249: 374-379) and the α-fetoprotein promoter (Campes and Tilghman (1989) Genes Dev . 3: 537-546).

The present invention further provides a recombinant expression vector containing a DNA molecule of the invention cloned in an expression vector in an antisense direction. That is, the DNA molecule is functionally linked to a regulatory sequence to enable expression (by transcription of the DNA molecule) of an RNA molecule that is antisense to HA mRNA. Regulatory sequences functionally linked to the nucleic acid cloned in the antisense direction may be chosen to allow for continuous expression of antisense RNA molecules in various cell types, such as, for example, viral promoters and / or enhancers, or the antisense RNA may be constitutive, Regulatory sequences may also be selected that allow for tissue-specific or cell type specific expression. Antisense expression vectors can be in the form of recombinant plasmids, phagemids or attenuated viruses that produce antisense nucleic acids under the control of highly efficient regulatory regions, the activity of which can be determined by the cell type into which the vector has been introduced. For controlling gene expression using antisense genes, see Weintraub, H. et al ., Antisense RNA as a molecular tool for genetic analysis, Reviews - Trends in Genetics , Vol. 1 (1) 1986).

Another aspect of the invention relates to a host cell into which the recombinant expression vector of the invention has been introduced. The terms "host cell" and "recombinant host cell" are used interchangeably herein. Such terms are understood not only to refer to specific subject cells but also to progeny or potential progeny of such cells. In fact, such progeny are not identical to parental cells, but may still fall within the scope of the term used herein, as any modification may occur due to mutations or environmental effects over generations.

The host cell can be any prokaryotic or eukaryotic cell. For example, HA proteins can be expressed in bacterial cells such as Corynebacterium glutamicum, insect cells, yeast or mammalian cells (eg, Chinese large mouse ovary cells (CHO) or COS cells). Other suitable host cells are known to those skilled in the art. Corynebacterium glutamicum related microorganisms that can be readily used as host cells for the nucleic acid and protein molecules of the present invention are described in Table 3.

Vector DNA can be introduced into prokaryotic or eukaryotic cells via conventional transformation or transfection techniques. As used herein, the terms “transformation”, “transfection”, “conjugation” and “transduction” refer to various known techniques for introducing foreign nucleic acids (eg, DNA) into host cells, and include calcium phosphate or Co-precipitation of calcium chloride, DEAE-dextran-via transfection, lipofection or electroporation. Suitable methods for transforming or transfecting host cells are described in Sambrook et al . ( Molecular Cloning: A Laboratory Manual. 2nd, ed., Cold Spring Harbor Laboratory , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989) and other experimental guides.

For stable transfection of mammalian cells, it is known that only a few minor cells insert heterologous DNA into their genome, depending on the expression vector and the transfection technique used. To identify and select these inserts, genes that encode selectable labels (eg, antibiotic resistance) are generally introduced into the host cell along with the gene of interest. Preferred selectable labels include those that confer drug resistance, such as G418, hygromycin and methotrexate. The nucleic acid encoding the selectable label may be introduced into a host cell on the vector encoding the HA protein, or may be introduced on a separate vector. Cells stably transfected with the introduced nucleic acid can be identified, for example, by drug selection (eg, cells incorporating selectable marker genes will survive, but other cells will die).

To prepare a homologous recombinant microorganism, a vector containing at least a portion of the HA gene that has been modified (eg, functionally disrupted) of the HA gene by deletion, addition or substitution is introduced. Preferably this HA gene is the Corynebacterium glutamicum HA gene, but may be a homologue of mammalian, yeast or insect origin as well as homologues from related bacteria. In a preferred embodiment, the vector is designed such that by homologous recombination the endogenous HA gene is functionally disrupted (ie, no longer encodes a functional protein, also referred to as a "knock-out" vector). Alternatively, the vector is designed to homologously recombine the mutated or otherwise modified endogenous HA gene but still encode a functional protein (ie the upstream regulatory region may be modified to alter the expression of the endogenous HA protein). . Homologous recombination vectors have additional nucleic acids of the HA gene flanking the 5 'and 3' sides of the modified HA gene fragment so that homologous recombination occurs between the foreign HA gene carried by the vector and the endogenous HA gene in the microorganism. do. The added flanking HA nucleic acid has a length sufficient to successfully homologous recombination with the endogenous gene. Typically, several kilobases of flanking DNA (both 5 'and 3' ends) are included in the vector (eg, Thomas, KR, and Capecchi, MR (1987), which describe homologous recombination vectors. ) Cell 51: 503). The vector is introduced into the microorganism (eg, by electroporation), and cells in which the introduced HA gene and the endogenous HA gene are homologous and recombined are selected by known techniques.

In other embodiments, recombinant microorganisms can be prepared comprising a selection system to control the expression of the introduced gene. For example, if the HA gene is included and located under the control of a lac operon on a vector, expression of the HA gene is possible only in the presence of IPTG. Such control systems are known in the art.

Host cells of the invention, such as cultured prokaryotic or eukaryotic host cells, can produce (ie, express) HA proteins. Accordingly, the present invention further provides a method for producing HA protein using the host cell of the present invention. In one embodiment, the method comprises a host cell of the invention (recombinant expression vector encoding the HA protein introduced, or a gene encoding a wild type or modified HA protein introduced into the genome) until the HA protein is produced. Culturing in a suitable medium. In other embodiments, the method further comprises isolating HA protein from said medium or host cell.

C. Uses and Methods of the Invention

The nucleic acid molecules, proteins, protein homologues, fusion proteins, primers, vectors and host cells described herein may be used to identify corynebacterium glutamicum and related organisms, genomic mapping of organisms associated with corynebacterium glutamicum, Identification and location of target sequences of Corynebacterium glutamicum, evolutionary studies, determination of HA protein sites required for function, regulation of HA protein activity, regulation of metabolism of one or more inorganic compounds, one or more aromatic or aliphatic compounds Can be used in one or more of methods such as controlling the modification or degradation of cells, regulating cell wall synthesis or rearrangement, regulating enzyme activity or proteolytic activity, and regulating cell production of target compounds such as fine chemicals. First, it can be used to identify the presence of Corynebacterium glutamicum or its close homologues. It can also be used to confirm the presence of Corynebacterium glutamicum or its close homologues in a mixed microbial population. The present invention relates to a large number of cory that can be identified by the presence of the organism by examining genomic DNA extracted from single or mixed microbial cultures using probes spanning gene regions unique to Corynebacterium glutamicum under stringent conditions. Nucleic acid sequences of the Nebacterium glutamicum gene are provided. Corynebacterium glutamicum itself is not pathogenic, but is associated with pathogenic species such as Corynebacterium diphtheriae . Detection of such organisms is of great clinical importance.

Nucleic acid and protein molecules of the invention can function as markers of specific regions of the genome. This function is useful not only for genome mapping but also for the study of Corynebacterium glutamicum proteins. For example, the Corynebacterium glutamicum genome can be cut to incubate fragments with DNA binding proteins to identify genomic regions to which specific DNA binding proteins of Corynebacterium glutamicum bind. The region that binds to the protein can also be irradiated with a nucleic acid molecule of the invention, preferably a label that can be easily detected. The binding of nucleic acid molecules to such genomic fragments allows the fragments to be located on the genomic map of Corynebacterium glutamicum and, if performed multiple times with different enzymes, can quickly and easily determine the nucleic acid sequences to which the proteins bind. have. In addition, the nucleic acid molecules of the present invention may have sufficient homology with the sequences of the associated species and can be used as markers for creating genomic maps of associated bacteria, such as Brevibacterium lactofermentum .

In addition, the HA nucleic acid molecules of the present invention are useful for evolution and protein structure studies. The processes involved in maintaining adaptation and homeostasis involving the molecules of the invention are used by a wide variety of species. The evolutionary association of an organism can be assessed by comparing the sequence of nucleic acid molecule sequences of the present invention with nucleic acid molecule sequences encoding enzymes similar to other organisms. Similarly, such comparisons can assess whether a sequence region is conserved, which can help determine protein regions essential for enzyme function. This determination method is useful for protein engineering and can indicate whether it is possible to tolerate the induced mutations without loss of protein function.

By manipulation of the HA nucleic acid molecule of the present invention, an HA protein having a function different from that of a wild type HA protein can be produced. Such proteins may be improved in efficiency or activity, may be present in excess than normal cells, or may be reduced in efficiency or activity.

Modulation of the activity or number of HA proteins associated with cell wall biosynthesis or rearrangement can affect the productivity, yield and / or efficiency of the production of one or more fine chemicals from Corynebacterium glutamicum. For example, by changing the activity of these proteins, the structure or thickness of the cell wall can be controlled. The cell wall acts primarily as a protective device against osmotic lysates and external damage sources. By changing the cell wall, it is possible to increase the ability of the microorganisms to withstand the mechanical and shear stress stresses that may occur during the growth of Corynebacterium glutamicum in large-scale fermentors. In addition, since each Corynebacterium glutamicum cell is surrounded by a thick cell wall, a substantial portion of the biomass is composed of the cell wall during large-scale culture. The growth rate of Corynebacterium glutamicum can be improved by increasing the rate at which cell walls are synthesized or by activating cell wall synthesis (via genetic manipulation of the HA cell wall of the invention). Similarly, cell wall production can be increased globally by reducing the activity or number of proteins involved in cell wall degradation or by reducing the inhibition of cell wall biosynthesis. Increasing the number of viable Corynebacterium glutamicum cells (which can be achieved by any of the protein changes described above) results in an increase in the number of cells that produce the desired fine chemicals in large fermenter cultures. It can increase the yield or efficiency of fine chemical production.

In addition, modulating the activity or number of Corynebacterium glutamicum HA proteins involved in the modification or degradation of aromatic or aliphatic compounds may directly or indirectly affect the production of one or more microchemicals from these cells. Can be. Modifications or degradation products of certain aromatic or aliphatic compounds are preferred microchemicals (e.g., organic acids or modified aromatic and aliphatic compounds) and therefore such modifications (e.g. hydroxylation, methylation or isomerization) or degradation reactions. The yield of the desired compound can be increased by modifying the enzymes that perform this. Similarly, by reducing the activity or number of proteins associated with pathways that further degrade the modified or disrupted reaction product, the yield of these microchemicals from the culture of Corynebacterium glutamicum cells can be improved. Can be.

The modifying and degrading enzymes of these aromatic and aliphatic compounds are themselves fine chemicals. These enzymes in purified form are used to degrade aromatic and aliphatic compounds (eg, toxic compounds such as petroleum products); It is used for bioremediation of contaminated sites, for the genetic engineering of waste, or for the large scale production of desired modified aromatic or aliphatic compounds or decay products and the production of economically reasonable scale, some of which may be used for other fine chemicals in culture. It can be conveniently used as a carbon source or energy source for material-producing compounds (Faber, K. (1995) Biotransformations in Organic Chemistry, Springer: Berlin and its references; and Roberts, SM, ed. (1992-1996) Preparative Biotransformations, Wiley: Chichester, and references therein). By changing genes such that the regulation of these proteins by other cellular mechanisms is reduced or reduced, the overall activity or number of these proteins can be increased to improve the yield of fine chemicals as well as to improve the activity of recovered proteins. .

In addition, modifications to the enzymes that modify and degrade these aromatic and aliphatic compounds can have an indirect effect on the productivity of one or more fine chemicals. Many aromatic and aliphatic compounds (such as those that may be present as contaminants in the culture medium or as waste from the cells themselves) are toxic to the cells, so that these compounds can be modified and / or removed to facilitate removal or destruction. Degradation may increase cell viability. In addition, these enzymes can modify or degrade aromatic and aliphatic compounds to allow the resulting products to enter the cell's normal carbon metabolic pathways, thus allowing cells to use these compounds as alternative carbon sources or energy sources. . For large scale cultures, when the amount of optimal carbon source is limited, these enzymes provide a way for cells to continue to grow and divide using aromatic or aliphatic compounds as nutrients. In each case, increasing the number of Corynebacterium glutamicum cells in the culture producing the desired fine chemicals increases the yield or efficiency of the fine chemical (s) production.

In addition, altering the activity or number of HA proteins involved in metabolism of inorganic compounds can directly or indirectly affect the productivity of one or more microchemicals from Corynebacterium glutamicum or related bacterial cultures. For example, many preferred fine chemicals such as nucleic acids, amino acids, cofactors and vitamins (eg, thiamin, biotin and lipoic acid) cannot be synthesized without inorganic molecules such as sulfur, nitrates, sulfates and iron. Inorganic compound metabolic proteins of the invention allow cells to obtain these molecules from a variety of inorganic compounds and convert them into a variety of fine chemical biosynthetic pathways. Thus, by increasing the activity or number of enzymes involved in the metabolism of these inorganic compounds, the yield of various fine chemicals from Corynebacterium glutamicum cells containing altered proteins may be increased by increasing the supply of inorganic molecules, which may be limited and The efficiency can be increased directly. In addition, altering the activity or number of inorganic compound metabolizing enzymes of the present invention allows Corynebacterium glutamicum to better utilize limited inorganic compounds, or amino acids, vitamins, which are essential for sustained cell growth and replication, It allows the use of inorganic compounds that are not optimal for the synthesis of cofactors or nucleic acids. By improving the viability of these cells in large scale cultures, the number of Corynebacterium glutamicum cells that produce one or more microchemicals in the culture can be increased, thus yielding the yield of one or more microchemicals or The efficiency is increased.

Corynebacterium glutamicum enzymes for universal processes are in themselves preferred microchemicals. Specific properties of enzymes (ie, site specificity and stereospecificity) allow them to act as useful catalysts in in vitro chemical reactions. Incubating whole Corynebacterium glutamicum cells with an appropriate substrate to produce the desired products in the cells by enzymes, or overexpressing the desired enzymes to corynebacterium glutamicum cultures (or cultures of related bacteria) After purification, it can be used for in vitro reactions (in solution or in a fixed stationary phase) under industrial conditions. In each case, the enzyme may be a native Corynebacterium glutamicum protein or may be mutated to have altered activity. Such enzymes are typically used as catalysts in the chemical industry (eg synthetic organic chemistry), as food additives, as animal feed ingredients, for fruit processing, for leather preparation, in surfactants, in assays and in medicine, And Yamada, H. (1993) "Microbial reactions for the production of useful organic compounds," Chimica 47: 5-10; Roberts, SM (1998) Preparative biotransformations: the employment of enzymes and whole- cells in synthetic chemistry, "J. Chem. Soc. Perkin Trans. 1: 157-169; Zaks, A. and Dodds, DR (1997)" Application of biocatalysis and biotransformations to the synthesis of pharmaceuticals, "DDT 2: 513- 531; Roberts, SM and Williamson, NM (1997) "The use of enzymes for the preparation of biologically active natural products and analogues in optically active form," Curr. Organ. Chemistry 1: 1-20; Faber, K. (1995 ) Biotransformations in Organic Chemistry, Springer: Berlin; Roberts, SM, ed. (1992-19 96) Preparative Biotransformations, Wiley: Chichester; Cheetham, PSJ (1995) "The applications of enzymes in industry" in: Handbook of Enzyme Biotechnology, 3 ^rd ed., Wiseman, A., ed., Elis: Horwood, pp. 419-552; And Ullmann's Encyclopedia of Industrial Chemistry (1987), Vol. A9, Enzymes, pp. 390-457). Thus, by increasing the activity or number of these enzymes, one can increase the cell's ability to convert the supplied substrate into the desired product, or overproduce these enzymes for increased yield in large scale culture. In addition, by mutating these proteins, it is possible to eliminate feedback inhibition or other inhibitory cell regulation so that the enzyme is much more generated and activated by the cells, thus yielding, productivity or efficiency of fine chemical production from large cultures. This will be much bigger. In addition, manipulation of these enzymes can alter one or more activities of the Corynebacterium glutamicum metabolic pathway, such as the pathway for biosynthesis or secretion of one or more microchemicals.

Inducing mutations to alter the activity or number of the proteolytic enzymes of the present invention directly or indirectly affects the yield, productivity and / or production efficiency of producing one or more microchemicals from Corynebacterium glutamicum. Can have For example, by increasing the activity or number of these proteins, large-scale cultivation is due to an increase in cellular capacity to rapidly degrade proteins that are misfolded in response to high temperatures, suboptimal pH, and other stresses that may occur during fermenter cultures. May increase the viability of bacteria. As the number of cells in the culture increases, the yield or efficiency for the production of one or more desired fine chemicals may increase because of the relatively large number of cells producing the fine chemicals in the culture. In addition, Corynebacterium glutamicum cells have several cell-surface proteases, which act to break down external nutrients into molecules that can be quickly incorporated into cells as carbon / energy sources or other types of nutrients. . Increasing the activity or number of these enzymes increases the recovery of degradation and increases the amount of available nutrients, thereby improving cell growth or productivity. Thus, modification of the protease of the present invention has an indirect effect on the production of fine chemicals of Corynebacterium glutamicum.

Where one or more proteases of the present invention are involved in the production or degradation of the desired fine chemical, the modification of the protease may have a more direct impact on the production of the fine chemical. By reducing the activity of microchemicals or proteases that degrade proteins associated with the synthesis of microchemicals, the amount of microchemicals can be increased (due to reduced degradation of the compound or increased synthesis). Similarly, similar results can be obtained by increasing the activity of microchemicals or proteases that produce proteins involved in the breakdown of microchemicals: corynebacterium containing genetically engineered proteins. Increasing the amount of desired microchemical from glutamicum cells.

The above mentioned mutagenesis for the HA protein to increase the yield of fine chemicals from Corynebacterium glutamicum is not limiting, and variations of this method will be apparent to those skilled in the art. Use of such methods, including the mechanisms disclosed herein, and the use of the nucleic acids and protein molecules of the present invention to produce Corynebacterium glutamicum or related strains expressing mutated HA nucleic acids and protein molecules, yielding the desired compounds. To improve production and / or production rates. The compounds of interest are coryne including the end product of the biosynthetic pathway and intermediates of the natural metabolic pathway, as well as the molecules produced by the Corynebacterium glutamicum strain, which do not inherently occur in Corynebacterium glutamicum metabolism. Any product produced by bacterium glutamicum.

In addition, the present invention is illustrated by the following examples, but should not be construed as being limited thereto. All references, patent applications, patents, all contents of published patent applications, tables and sequences listed herein are considered to be incorporated by reference.

Example 1 Prep of Whole Genome DNA of Corynebacterium glutamicum ATCC 13032

Corynebacterium glutamicum (ATCC 13032) cultures in BHI medium (Difco) were grown overnight with vigorous shaking at 30 ° C. Cells were harvested by centrifugation to discard the supernatant and cells were resuspended in 5 ml Buffer-I (corresponding to 5% of the initial volume of culture—all volumes indicated for 100 ml of culture volume). The composition of Buffer-I was as follows: 140.34 g / L sucrose, 2.46 g / L MgS0 ₄ × 7 H ₂ O, 10 ml / L KH ₂ PO ₄ solution (100 g / L, pH 6.7 using KOH) ), 50 ml / L M12 concentrate (10 g / L (NH ₄ ) ₂ SO ₄ , 1 g / L NaCl, 2 g / L MgSO ₄ × 7 H ₂ O, 0.2 g / L CaCl ₂ , 0.5 g / L yeast extract (from Difco), 10 ml / L trace element mixture (200 mg / L FeSO ₄ × H ₂ O, 10 mg / L ZnSO ₄ × 7 H ₂ O, 3 mg / L MnCl ₂ × 4 H ₂ O, 30 mg / LH ₃ BO ₃ , 20 mg / L CoCl ₂ × 6 H ₂ O, 1 mg / L NiCl ₂ × 6 H ₂ O, 3 mg / L Na ₂ MoO ₄ × 2 H ₂ O, 500 mg / L complexing agent (EDTA or citric acid), 100 ml / L vitamin mixture (0.2 mg / L biotin, 0.2 mg / L folic acid, 20 mg / L p-amino benzoic acid, 20 mg / L riboflavin, 40 mg / L ca Pantothenate, 140 mg / L nicotinic acid, 40 mg / L pyridoxol hydrochloride, 200 mg / L myo-inositol) To this suspension lysozyme was added at a final concentration of 2.5 mg / ml, approximately 4 hours at 37 ° C. Incubated for a while, then disrupt the cell wall and centrifuge The protoplasts were collected, and the pellet was washed once with 5 ml Buffer-I and once with 5 ml TE-buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8). Resuspend in buffer and add 0.5 ml SDS solution (10%) and 0.5 ml NaCl solution (5 M) Proteinase K was added to a final concentration of 200 μg / ml, and then the suspension was 37 ° C. The DNA was purified by extraction with phenol, phenol-chloroform-isoamyl alcohol and chloroform-isoamyl alcohol using standard methods, followed by 1/50 volume of 3 M sodium acetate and DNA was precipitated by the addition of 2 volumes of ethanol, then incubated for 30 minutes at -20 ° C and centrifuged for 30 minutes at 12,000 rpm with a high speed centrifuge using SS34 rotor (Sorvall). The DNA was dissolved in 1 ml TE-buffer containing 20 μg / ml RNaseA and dialyzed in 1000 ml TE-buffer at 4 ° C. for at least 3 hours. During dialysis, the buffer was changed three times. 0.4 ml aliquots of the dialyzed DNA solution were added, and 0.4 ml 2 M LiCl and 0.8 ml ethanol were added. DNA was collected by incubation at −20 ° C. for 30 minutes and then centrifuged (13,000 rpm, Bioofuge Fresco, Hanau Heraeus, Germany). DNA pellets were dissolved in TE-buffer. DNA prepared in this manner was used for all purposes, such as Southern blotting or genome library construction.

Example 2: Genome Bank Construction of Corynebacterium glutamicum (ATCC13032) in Escherichia Coli

Using prepared DNA as described in Example 1, known and established methods (eg, Sambrook, J. et al. (1989) "Molecular Cloning: A Laboratory Manual", Cold Spring Harbor Laboratory) Press or see Ausubel, FM et al. (1994) "Current Protocols in Molecular Biology", John Wiley & Sons).

Any plasmid or cosmid could be used. In particular, plasmid pBR322 (Sutcliffe, J.G. (1979) Proc. Natl. Acad. Sci. USA, 75: 3737-3741); pACYC177 (Change & Cohen (1978) J. Bacteriol 134: 1141-1156), plasmids of the pBS family (pBSSK +, pBSSK-, etc .; Stratagene, LaJolla, USA), or SuperCos1 (Stratagene, LaJolla, USA) ) Or Lorist6 (Gibson, TJ, Rosenthal A. and Waterson, RH (1987) Gene 53: 283-286).

Example 3: DNA sequencing and functional analysis using a computer

DNA sequencing according to standard methods, especially chain termination methods using ABI377 sequencers, using genome banks as described in Example 2 (see, eg, Fleischman, RD et al. (1995) "Whole- DNA sequencing was performed by genome Random Sequencing and Assembly of Haemophilus Influenzae Rd., Science, 269: 496-512. '-GTAAAACGACGGCCAGT-3'.

Example 4: In vivo Mutagenesis

In vivo mutagenesis of Corynebacterium glutamicum has been shown to affect E. coli or other microorganisms (e.g., Bacillus spp. Or Saccharomyces cerevisiae ) that have impaired the ability to maintain complete genetic information . Yeast) can be performed by passage of plasmid (or other vector) DNA. Typical mutagenesis strains include genes related to DNA repair systems (eg, mutHLS, mutD, mutT, etc .; for reference, see Rupp, WD (1996) DNA repair mechanisms, in: Escherichia coli and Salmonella , pp. 2277- 2294, ASM: Washington). Such strains are known to those skilled in the art. The use of such strains is described, for example, in Greener, A. and Callahan, M. (1994) Strategies 7: 32-34.

Example 5: DNA transfer between E. coli and Corynebacterium glutamicum

Several Corynebacterium and Brevibacterium species contain endogenous plasmids that autonomously replicate (eg, pHM1519 or pBL1, etc.) (see, for example, Martin, JF et al. (1987) Biotechnology, 5: 137-146). Shuttle vectors for use with E. coli and Corynebacterium glutamicum are standard vectors used for E. coli, with the origin of replication for Corynebacterium glutamicum and the appropriate markers from Corynebacterium glutamicum Sambrook, J. et al. (1989), "Molecular Cloning: A Laboratory Manual", Cold Spring Harbor Laboratory Press, or Ausubel, FM et al. (1994) "Current Protocols in Molecular Biology", John Wiley & Sons]). The origin of replication is preferably obtained from endogenous plasmids isolated from Corynebacterium and Brevibacterium species. In particular, genes used as transformation markers for these species may be kanamycin resistance genes (eg, derived from Tn5 or Tn903 transposons, etc.) or chloramphenicol resistance genes (Winnacker, EL (1987) "From Genes to Clones-Introduction". to Gene Technology, VCH, Weinheim]) There are numerous documents on the construction of a wide variety of shuttle vectors that can be cloned in both E. coli and Corynebacterium glutamicum and used for a variety of purposes including gene overexpression. (For reference, see, eg, Yoshihama, M. et al. (1985) J. Bacteriol. 162: 591-597, Martin JF et al. (1987) Biotechnology, 5: 137-146) and See Eikmanns, BJ et al. (1991) Gene, 102: 93-98).

Using standard methods, genes of interest can be cloned into one of the shuttle vectors described above and such hybrid vectors can be introduced into Corynebacterium glutamicum strains. Transformation of Corynebacterium glutamicum is known as protoplast transformation (Kastsumata, R. et al. (1984) J. Bacteriol. 159: 306-311), electroporation (Liebl, E. et al. (1989) FEMS Microbiol.Letters, 53: 399-303) and conjugation methods when certain vectors are used (eg, Schaefer, A et al. (1990) J. Bacteriol. 172: 1663). -1666). In addition, prep the plasmid DNA from Corynebacterium glutamicum (using standard methods known in the art) and transform it into Escherichia coli to transfer the shuttle vector to E. coli for use in Corynebacterium glutamicum. It may be. This transformation step can be performed using standard methods, but it is advantageous to use Mcr-deficient E. coli strains such as NM522 (Gough & Murray (1983) J. Mol. Biol. 166: 1-19) and the like. Do.

Example 6: Measurement of Expression of Mutant Proteins

Observation of the activity of the mutated protein in the transformed host cell revealed that the mutant protein was expressed in a similar manner and in a similar amount to the wild type protein. Useful methods for determining the transcriptional level of the mutant gene (indicator for the amount of mRNA available for translation into the gene product) include Northern blotting (see, for example, Ausubel et al. (1988) Current Protocols in Molecular). Biology, Wiley: New York), which extracts the whole RNA of a biological culture, develops it on a gel, transfers it to a stable matrix, and then binds to the gene of interest to detect a tag (usually Incubating with a primer labeled with a radioactive or chemiluminescent tag), the binding of the probe and its binding amount also indicate the presence and amount of mRNA of the gene of interest. This information is evidence of the degree of transcription of the mutant gene. Intracellular total RNA of Corynebacterium glutamicum is described, for example, in Bormann, E.R. et al. (1992) Mol. Microbiol. 6: 317-326 can be prepared using a variety of methods known in the art.

To assess the presence or relative amount of a protein translated from this mRNA, standard techniques such as western blotting can be used (see, for example, Ausubel et al. (1988) Current Protocols in Molecular Biology, Wiley). New York). In this method, the whole protein in the cell is extracted, separated by gel electrophoresis, and then transferred to a matrix such as nitrocellulose and incubated with a probe such as an antibody that specifically binds to a desired protein. Typically, these probes are tagged with chemiluminescent or colorimetric labels that can be easily detected. The presence and amount of the label observed indicates the presence and amount of the desired mutant protein in the cell.

Example 7: Growth of Genetically Modified Corynebacterium glutamicum-Medium and Culture Conditions

Genetically modified corynebacteria are cultured in synthetic or natural growth media. Numerous different growth media of Corynebacteria are known in the art and are also readily available (Lieb et al. (1989) Appl. Microbiol. Biotechnol., 32: 205-210, von der Osten (1998) Biotechnology Letters, 11: 11-16, German Patent No. 4,120,867, Liebl (1992) "The Genus Corynebacterium", in: The Procaryotes, Volume II, Balows, A. et. al., Editors. Springer-Verlag]. The medium consists of one or more carbon sources, nitrogen sources, inorganic salts, vitamins and trace elements. Preferred carbon sources are sugars such as monosaccharides, disaccharides, or polysaccharides. For example, glucose, fructose, mannose, galactose, ribose, sorbose, ribulose, lactose, maltose, sucrose, raffinose, starch or cellulose serve as very good carbon sources. In addition, sugar may be supplied to the medium through complex compounds such as molasses or other by-products generated during sugar purification. It may also be advantageous to feed a mixture of different carbon sources. Other possible carbon sources are alcohols and organic acids such as methanol, ethanol, acetic acid or lactic acid. Nitrogen sources are typically organic or inorganic nitrogen compounds, or materials containing such compounds. Examples of nitrogen sources include ammonia gas or ammonium salts such as NH ₄ Cl or (NH ₄ ) ₂ SO ₄ , NH ₄ 0H, nitrates, ureas, amino acids or corn lipsor, soy flour, soy protein, yeast extract, meat Complex nitrogen sources such as extracts.

Inorganic salt compounds that may be included in the medium include calcium, magnesium, sodium, cobalt, molybdenum, potassium, manganese, zinc, copper and iron hydrochlorides, phosphates, or sulfates. Chelating compounds can be added to the medium to keep metal ions in solution. Particularly useful chelating compounds include dihydroxyphenols such as catechol or protocatechuate, organic acids such as citric acid, and the like. In addition, the medium typically contains other growth factors such as vitamins or growth promoters, examples of which include biotin, riboflavin, thiamine, folic acid, nicotinic acid, pantothenate and pyridoxine. Growth factors and salts often occur in complex media components such as yeast extract, molasses, corn soak and the like. The exact composition of the media compound will vary greatly with subsequent experiments and will be determined separately for each particular case. Information on media optimization can be found in Applied Microbiol. Physiology, A Practical Approach (Editors. PM Rhodes, PF Stanbury, IRL Press (1997) pp. 53-73, ISBN 0 19 963577 3). It is also possible to select from growth media from the manufacturer, such as Standard 1 (Merck) or BHI (Leads in Grain, DIFCO).

All media components are sterilized by heat treatment (20 minutes at 1.5 bar and 121 ° C.) or sterile filtration. These components can be sterilized all at once or they can be sterilized separately as needed. All media components may be present from the beginning of the culture, or optionally added continuously or batchwise.

Culture conditions are defined separately for each experiment. The temperature should be in the range between 15 ° C and 45 ° C. The temperature can be kept constant or changed during the experiment. The pH of the medium should be in the range of 5 to 8.5, preferably about 7.0, and can be maintained by adding buffer to the medium. Examples of buffers that can be used for this purpose include potassium phosphate buffers and the like. Synthetic buffers such as MOPS, HEPES, ACES, etc. may alternatively or simultaneously be used. It is also possible to keep the pH of the culture constant by adding NaOH or NH ₄ OH during the culture. When complex media components such as yeast extracts are used, many complex compounds have high buffering performance, which may reduce the need for additional buffers. When the fermentor is used for microbial culture, the pH may be controlled using ammonia gas.

Incubation time ranges are typically several hours to several days. This time is chosen so that the amount of product that accumulates in the broth is maximum. The disclosed culture experiments can be performed in a variety of vessels, such as microtiter plates, glass tubes, glass flasks or glass or metal fermenters of different sizes. To screen numerous clones, microorganisms must be cultured in microtiter plates, glass tubes or shake flasks with or without baffles. It is preferable to use the required growth medium by filling 10% (volume ratio) in a 100 ml shake flask. These flasks should be shaken (25 mm amplitude) on a rotary shaker in the speed range 100-300 rpm. The amount of loss due to evaporation can be reduced by keeping the atmosphere wet; Alternatively, the amount of loss due to evaporation must be corrected correctly.

When investigating genetically modified clones, control clones containing unmodified control clones or base plasmids without any inserts should also be analyzed. CM plates incubated at 30 ° C. (10 g / L glucose, 2.5 g / L NaCl, 2 g / L urea, 10 g / L polypeptone, 5 g / L yeast extract, 5 g / L meat extract, 22 g / Cells on agar plates, such as L agar, adjusted to pH 6.8 with 2 M NaOH), and the like are seeded in the medium to an OD ₆₀₀ 0.5-1.5. Medium inoculation is performed by introducing a saline suspension of Corynebacterium glutamicum cells from the CM plates or by adding pre-cultures of these bacteria.

Example 8 In Vitro Analysis of the Function of Mutant Proteins

Methods of measuring enzyme activity and reaction rate parameters are well established in the art. Experiments for measuring the activity of any given modified enzyme should be tailored to the inactivation of the wild type enzyme and one skilled in the art can do this well. Specific details regarding the structure, reaction rates, principles, methods, and applications of enzymes, as well as a general overview of enzymes and examples of methods for measuring activity of many enzymes, can be found in the following references: Dixon, M. and Webb, EC, (1979) Enzymes. Longmans: London; Fersht, (1985) Enzyme Structure and Mechanism. Freeman: New York, Walsh, (1979) Enzymatic Reaction Mechanisms. Freeman: San Francisco, Prix, NC, Stevens, L. (1982) Fundamentals of Enzymology. Oxford Univ. Press: Oxford, Boyer, PD, ed. (1983) The Enzymes, 3 ^rd ed. Academic Press: New York, Bisswanger, H., (1994) Enzymkinetik, 2 ^nd ed. VCH: Weinheim (ISBN 3527300325), Bergmeyer, HU, Bergmeyer, J., Grassl, M., Editors. (1983-1986) Methods of Enzymatic Analysis, 3 ^rd ed., Vol. I-XII, Verlag Chemie: Weinheim, and Ullmann's Encyclopedia of Industrial Chemistry (1987) Vol. A9, "Enzymes". VCH: Weinheim, pp. 352-363].

Protein activity that binds to DNA can be measured using a variety of well-established methods, such as DNA band-displacement assays (also called gel retardation assays). The effect of these proteins on the expression of other molecules is described in reporter gene analysis (see, eg, Kolmar, H. et al. (1995) EMBO J. 14: 3895-3904) and references cited therein. As shown in FIG. 2). Reporter gene analysis systems using enzymes such as β-galactosidase, green fluorescent proteins and many others are known and well established for application to both prokaryotic and eukaryotic cells.

Measurement of the activity of membrane transport proteins is described in Gennis, R.B. (1989) "Pores, Channels and Transporters", in Biomembranes, Molecular Structure and Function, Springer: Heidelberg, pp. 85-137, 199-234, and 270-322, and the like.

Example 9: Analysis of the Effect of Mutant Proteins on Desired Product Production

The effect of genetic modification on Corynebacterium glutamicum on the production of the desired compound (e.g., amino acid) may be achieved by culturing the modified microorganism under appropriate conditions (e.g., as described above), Media components and / or intracellular components involved in increased production of product (ie, amino acids) can be assessed by analysis. Such analytical techniques are known to those skilled in the art and include analytical chromatography methods such as spectroscopy, thin layer chromatography, various staining, enzymatic and microbiological methods, and high performance liquid chromatography methods (e.g., literature Ullman, Encyclopedia of Industrial Chemistry, Vol. A2, pp. 89-90 and pp. 443-613, VCH: Weinheim (1985), Fallon, A. et al., (1987) "Applications of HPLC in Biochemistry "in: Laboratory Techniques in Biochemistry and Molecular Biology, Vol. 17, Rehm et al. (1993) Biotechnology, Vol. 3, Chapter III:" Product recovery and purification ", page 469-714, VCH: Weinheim Belter, PA et al. (1988) Bioseparations: downstream processing for biotechnology, John Wiley and Sons, Kennedy, JF and Cabral, JMS (1992) Recovery processes for biological materials, John Wiley and Sons, Shaeiwitz, JA and Henry, JD (1988) Biochemical separations, in: Ulmann ' s Encyclopedia of Industrial Chemistry, Vol. B3, Chapter 11, page 1-27, VCH: Weinheim, and Dechow, F.J. (1989) Separation and purification techniques in biotechnology, Noyes Publications).

In addition to the determination of the final fermentation product, it is also possible to analyze other components of the metabolic pathways used to produce the desired compound, such as intermediates and by-products, and to determine the overall compound production efficiency. Analytical methods include measuring the content of nutrients (eg, sugars, hydrocarbons, nitrogen sources, phosphates and other ions) in the medium, determining the composition and growth of biomass, analyzing the production of common metabolites in biosynthetic pathways, and Gas measurements generated during fermentation. Standard methods for such measurements are described in Applied Microbial Physiology, A Practical Approach, P. M. Rhodes and P. F. Stanbury, Editors., IRL Press, pp. 103-129, 131-163, and 165-192 (ISBN: 0199635773) and references cited therein.

Example 10 Purification of Desired Product from Corynebacterium glutamicum Culture

Various methods known in the art can be used to recover the desired product from Corynebacterium glutamicum cells or supernatants of the cultures described above. If the desired product is not secreted from the cells, the cultures can be harvested by low speed centrifugation and the cells can be lysed by standard techniques such as mechanical force or ultrasound. Cell debris is removed by centrifugation and the supernatant portion containing the soluble protein is left for further purification of the desired compound. If the product is secreted from Corynebacterium glutamicum cells, the cultures are centrifuged at low speed to remove the cells, leaving the supernatant portion for further purification.

The supernatant portion by any of the two purification methods is subjected to chromatography using a suitable resin, so that the desired molecule remains on the chromatography resin but no impurities remain in the sample, or the impurities remain in the resin but the desired molecule remains. Does not remain. If desired, these chromatography steps can be repeated using the same or different chromatography resins. Those skilled in the art will be skilled in selecting suitable chromatography resins and applying them most effectively for the purification of particular molecules. The purified product can be concentrated by filtration or ultrafiltration and stored at a temperature where the stability of the product is maximum.

Purification methods known in the art are various and are not limited to the purification methods described above. Such purification techniques are described, for example, in Bailey, J.E. & Ollis, D.F. Biochemical Engineering Fundamentals, McGraw-Hill: New York (1986).

Identification and purity of the isolated compound can be assessed by standard techniques in the art. These include high performance liquid chromatography (HPLC), spectroscopy, staining, thin layer chromatography, NIRS, enzymatic analysis, or microbiological methods. The assay method is reviewed in the following literature: Patek et al. (1994) Appl. Environ. Microbiol. 60: 133-140, Malakhova et al. (1996) Biotekhnologiya 11: 27-32, Schmidt et al. (1998) Bioprocess Engineer. 19: 67-70, Ulmann's Encyclopedia of Industrial Chemistry, (1996) Vol. A27, VCH: Weinheim, pp. 89-90, pp. 521-540, pp. 540-547, pp. 559-566, pp. 575-581 and pp. 581-587, Michal, G. (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley and Sons, and Fallon, A. et al. (1987) Applications of HPLC in Biochemistry in: Laboratory Techniques in Biochemistry and Molecular Biology, Vol. 17].

Equivalent

Those skilled in the art will recognize or be able to as well as routine experimental methods, as well as many experimental methods equivalent to certain embodiments of the invention described herein. Such equivalent methods will be included in the following claims.

The information in Table 1 will be understood as follows:

In column 1, the relevant number set forth in “DNA-ID” indicates in each case the sequence number of the attached Sequence Listing. Thus, "5" in the "DNA-ID" column represents SEQ ID NO: 5.

In column 2, the relevant number described in "AA-ID" represents in each case the sequence number of the attached Sequence Listing. Thus, "6" in the "AA-ID" column represents SEQ ID NO: 6.

In column 3, "identifier" describes the apparent internal name for each sequence.

In column 4, the relevant number described in "AA-pos" indicates in each case the amino acid position of the polypeptide sequence in the "AA-ID" in the same row. Thus, "26" in column "AA-pos" refers to amino acid position 26 of the polypeptide sequence so represented. The number of positions started with the N terminus being +1.

In column 5, the relevant letters described in the "AA wild type" indicate in each case the amino acid indicated by the one-letter code at the position of the corresponding wild type strain shown in column 4.

In column 6, the relevant letters described in the "AA mutants" represent in each case the amino acids indicated by the one-letter code at the positions of the corresponding mutant strains shown in column 4.

In column 7, “function” describes the physiological function of the corresponding polypeptide sequence.

The one-letter code of proteinogenic amino acids is as follows:

A Alanine

C cysteine

D Aspartic Acid

E glutamic acid

F phenylalanine

G glycine

H histidine

I isoleucine

K lysine

L leucine

M methionine

N Asparagine

P proline

Q Glutamine

R arginine

S serine

T threonine

V valine

W tryptophan

Y tyrosine

SEQUENCE LISTING <110> BASF Aktiengesellschaft <120> Genes coding for homeostasis and adaptation proteins <130> O.Z. 0050/52975 <160> 168 <210> 1 <211> 1789 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1759) <223> RXA00048 <400> 1 gcgaaggcgt gggcataggg aaaaccttag ctgatctgcg gtgacttaaa tataaggggg 60 tggaatgggg gtattgtaaa atctgaaccc ttgttcattt atg aat cat gat tca 115 Met Asn His Asp Ser 1 5 gaa tgt gat cta gat aat gtt gtt cag ttc act att caa gaa ggg tta 163 Glu Cys Asp Leu Asp Asn Val Val Gln Phe Thr Ile Gln Glu Gly Leu 10 15 20 gat ccc atg tca ccc aat aac ttc gat acc gat gtc tgc atc gtc ggt 211 Asp Pro Met Ser Pro Asn Asn Phe Asp Thr Asp Val Cys Ile Val Gly 25 30 35 gga ggt cct acc gga acg ctc ctt gca gta ctg ctc ggc caa aaa ggt 259 Gly Gly Pro Thr Gly Thr Leu Leu Ala Val Leu Leu Gly Gln Lys Gly 40 45 50 cac cgc gtc acc atc ctg gaa aag tgg cca aca ttc tac gaa cga cct 307 His Arg Val Thr Ile Leu Glu Lys Trp Pro Thr Phe Tyr Glu Arg Pro 55 60 65 cgt gca gtc acc ttt gac cac gaa atc gca cgg atc ctt gga tac att 355 Arg Ala Val Thr Phe Asp His Glu Ile Ala Arg Ile Leu Gly Tyr Ile 70 75 80 85 ggc att gat tct gaa aac gac gaa gcc atc gat tac cac tcc gac agc 403 Gly Ile Asp Ser Glu Asn Asp Glu Ala Ile Asp Tyr His Ser Asp Ser 90 95 100 tac gac tgg aag aac gca gcg ggg gag acg ctt ttg gaa gtc gat tgg 451 Tyr Asp Trp Lys Asn Ala Ala Gly Glu Thr Leu Leu Glu Val Asp Trp 105 110 115 acc tcc atg aca gat tcc gga tgg cgc acc cga tac tgg ttc tac cag 499 Thr Ser Met Thr Asp Ser Gly Trp Arg Thr Arg Tyr Trp Phe Tyr Gln 120 125 130 cca gaa ctt gaa aag cgt ctg cgc gat ctg gcc ctg acc atg gat ttt 547 Pro Glu Leu Glu Lys Arg Leu Arg Asp Leu Ala Leu Thr Met Asp Phe 135 140 145 gta gat att cgc tgt ggc ttc acc gct gtc gga ttg tcc caa gat gaa 595 Val Asp Ile Arg Cys Gly Phe Thr Ala Val Gly Leu Ser Gln Asp Glu 150 155 160 165 aac tcc gcc atc att cac ggc att gtc act gat acc cca gag aac att 643 Asn Ser Ala Ile Ile His Gly Ile Val Thr Asp Thr Pro Glu Asn Ile 170 175 180 cca gca gat gct cag cgc gaa gat atc cga gcg aag tat gtc atc ggt 691 Pro Ala Asp Ala Gln Arg Glu Asp Ile Arg Ala Lys Tyr Val Ile Gly 185 190 195 gca gac gga gct aac agt ttc gtg cgt aac tcc ctt ggc tta gag atg 739 Ala Asp Gly Ala Asn Ser Phe Val Arg Asn Ser Leu Gly Leu Glu Met 200 205 210 aat gat ctt gga tac ttc ttc gac tgg ctg atc ctg gac ctc aag cca 787 Asn Asp Leu Gly Tyr Phe Phe Asp Trp Leu Ile Leu Asp Leu Lys Pro 215 220 225 act cag gac att gac tac gga aca gat cac tgg caa ctg tgt gat ccc 835 Thr Gln Asp Ile Asp Tyr Gly Thr Asp His Trp Gln Leu Cys Asp Pro 230 235 240 245 aag cgc cca acc acc atc gtt cct gga ggc ccc ggc cgc cga cgt tgg 883 Lys Arg Pro Thr Thr Ile Val Pro Gly Gly Pro Gly Arg Arg Arg Trp 250 255 260 gaa ttc atg gcg ctg cct ggt gaa gat ctc aag gaa ctc gct tct gaa 931 Glu Phe Met Ala Leu Pro Gly Glu Asp Leu Lys Glu Leu Ala Ser Glu 265 270 275 gaa agc gcg tgg aat cta ctt gag cca tgg gat gtc aca cct ggc aag 979 Glu Ser Ala Trp Asn Leu Leu Glu Pro Trp Asp Val Thr Pro Gly Lys 280 285 290 gcc att ctc gag cgc tcc gca gtg tat cga ttc caa gct cgc tgg gcc 1027 Ala Ile Leu Glu Arg Ser Ala Val Tyr Arg Phe Gln Ala Arg Trp Ala 295 300 305 cag gaa tgg cgc tcc gga aga gct ctc atc gca ggc gat gcc gct cac 1075 Gln Glu Trp Arg Ser Gly Arg Ala Leu Ile Ala Gly Asp Ala Ala His 310 315 320 325 ctc atg cca cct ttc gca ggt gaa ggc atg tgc gct ggc ctg aga gac 1123 Leu Met Pro Pro Phe Ala Gly Glu Gly Met Cys Ala Gly Leu Arg Asp 330 335 340 tca ctg gcg ttg gca tgg cgt ctt gat ttg gtg ctg agc gga aaa tca 1171 Ser Leu Ala Leu Ala Trp Arg Leu Asp Leu Val Leu Ser Gly Lys Ser 345 350 355 gat gat gca ttg cta gac acc tac gga gaa gaa cgc cgc gaa cac gtc 1219 Asp Asp Ala Leu Leu Asp Thr Tyr Gly Glu Glu Arg Arg Glu His Val 360 365 370 cac tac tac att gat ttc tcc atg gac ctg ggc aat gtc atc tgc att 1267 His Tyr Tyr Ile Asp Phe Ser Met Asp Leu Gly Asn Val Ile Cys Ile 375 380 385 act gat gaa gat gaa gca cgt ttg cgc gat gag cgc atg att aaa gag 1315 Thr Asp Glu Asp Glu Ala Arg Leu Arg Asp Glu Arg Met Ile Lys Glu 390 395 400 405 ctt gaa gca caa gac ggg gtc cct gtt aat acc gat gtc gca cac ttg 1363 Leu Glu Ala Gln Asp Gly Val Pro Val Asn Thr Asp Val Ala His Leu 410 415 420 gga ccg gga att tgg gat aaa gat tct tcc cat ggt ggc gag cta gcg 1411 Gly Pro Gly Ile Trp Asp Lys Asp Ser Ser His Gly Gly Glu Leu Ala 425 430 435 aag cag ggc ata gtg gaa tac caa ggt cga aag gcg cgt ttc gac gac 1459 Lys Gln Gly Ile Val Glu Tyr Gln Gly Arg Lys Ala Arg Phe Asp Asp 440 445 450 gca gtc ggc cgt ggc tgg gca gtt tta ggc ctc aac act gat cca cga 1507 Ala Val Gly Arg Gly Trp Ala Val Leu Gly Leu Asn Thr Asp Pro Arg 455 460 465 gaa gtg ctt gat gag gat tcg ctt gtg gca ctt gac gcc atc ggt gca 1555 Glu Val Leu Asp Glu Asp Ser Leu Val Ala Leu Asp Ala Ile Gly Ala 470 475 480 485 atc gtc gaa tca gta ggt gat gca act tcc gca gtt tta gat gtt gaa 1603 Ile Val Glu Ser Val Gly Asp Ala Thr Ser Ala Val Leu Asp Val Glu 490 495 500 ggt ctt tac act cgc tgg ctc aag gaa gcc ggg gca aca ttt att att 1651 Gly Leu Tyr Thr Arg Trp Leu Lys Glu Ala Gly Ala Thr Phe Ile Ile 505 510 515 acc cgc ccc gat ttc tac gtc tat tcc aca gca gtg gac gct gaa caa 1699 Thr Arg Pro Asp Phe Tyr Val Tyr Ser Thr Ala Val Asp Ala Glu Gln 520 525 530 ctt caa aca cag att aag cag cta tcg gat cta ctt cac ctc aac tca 1747 Leu Gln Thr Gln Ile Lys Gln Leu Ser Asp Leu Leu His Leu Asn Ser 535 540 545 gtt gtc gga gca taggagctaa aaatgtcttt acagttcgat 1789 Val Val Gly Ala 550 <210> 2 <211> 553 <212> PRT <213> Corynebacterium glutamicum <400> 2 Met Asn His Asp Ser Glu Cys Asp Leu Asp Asn Val Val Gln Phe Thr 1 5 10 15 Ile Gln Glu Gly Leu Asp Pro Met Ser Pro Asn Asn Phe Asp Thr Asp 20 25 30 Val Cys Ile Val Gly Gly Gly Pro Thr Gly Thr Leu Leu Ala Val Leu 35 40 45 Leu Gly Gln Lys Gly His Arg Val Thr Ile Leu Glu Lys Trp Pro Thr 50 55 60 Phe Tyr Glu Arg Pro Arg Ala Val Thr Phe Asp His Glu Ile Ala Arg 65 70 75 80 Ile Leu Gly Tyr Ile Gly Ile Asp Ser Glu Asn Asp Glu Ala Ile Asp 85 90 95 Tyr His Ser Asp Ser Tyr Asp Trp Lys Asn Ala Ala Gly Glu Thr Leu 100 105 110 Leu Glu Val Asp Trp Thr Ser Met Thr Asp Ser Gly Trp Arg Thr Arg 115 120 125 Tyr Trp Phe Tyr Gln Pro Glu Leu Glu Lys Arg Leu Arg Asp Leu Ala 130 135 140 Leu Thr Met Asp Phe Val Asp Ile Arg Cys Gly Phe Thr Ala Val Gly 145 150 155 160 Leu Ser Gln Asp Glu Asn Ser Ala Ile Ile His Gly Ile Val Thr Asp 165 170 175 Thr Pro Glu Asn Ile Pro Ala Asp Ala Gln Arg Glu Asp Ile Arg Ala 180 185 190 Lys Tyr Val Ile Gly Ala Asp Gly Ala Asn Ser Phe Val Arg Asn Ser 195 200 205 Leu Gly Leu Glu Met Asn Asp Leu Gly Tyr Phe Phe Asp Trp Leu Ile 210 215 220 Leu Asp Leu Lys Pro Thr Gln Asp Ile Asp Tyr Gly Thr Asp His Trp 225 230 235 240 Gln Leu Cys Asp Pro Lys Arg Pro Thr Thr Ile Val Pro Gly Gly Pro 245 250 255 Gly Arg Arg Arg Trp Glu Phe Met Ala Leu Pro Gly Glu Asp Leu Lys 260 265 270 Glu Leu Ala Ser Glu Glu Ser Ala Trp Asn Leu Leu Glu Pro Trp Asp 275 280 285 Val Thr Pro Gly Lys Ala Ile Leu Glu Arg Ser Ala Val Tyr Arg Phe 290 295 300 Gln Ala Arg Trp Ala Gln Glu Trp Arg Ser Gly Arg Ala Leu Ile Ala 305 310 315 320 Gly Asp Ala Ala His Leu Met Pro Pro Phe Ala Gly Glu Gly Met Cys 325 330 335 Ala Gly Leu Arg Asp Ser Leu Ala Leu Ala Trp Arg Leu Asp Leu Val 340 345 350 Leu Ser Gly Lys Ser Asp Asp Ala Leu Leu Asp Thr Tyr Gly Glu Glu 355 360 365 Arg Arg Glu His Val His Tyr Tyr Ile Asp Phe Ser Met Asp Leu Gly 370 375 380 Asn Val Ile Cys Ile Thr Asp Glu Asp Glu Ala Arg Leu Arg Asp Glu 385 390 395 400 Arg Met Ile Lys Glu Leu Glu Ala Gln Asp Gly Val Pro Val Asn Thr 405 410 415 Asp Val Ala His Leu Gly Pro Gly Ile Trp Asp Lys Asp Ser Ser His 420 425 430 Gly Gly Glu Leu Ala Lys Gln Gly Ile Val Glu Tyr Gln Gly Arg Lys 435 440 445 Ala Arg Phe Asp Asp Ala Val Gly Arg Gly Trp Ala Val Leu Gly Leu 450 455 460 Asn Thr Asp Pro Arg Glu Val Leu Asp Glu Asp Ser Leu Val Ala Leu 465 470 475 480 Asp Ala Ile Gly Ala Ile Val Glu Ser Val Gly Asp Ala Thr Ser Ala 485 490 495 Val Leu Asp Val Glu Gly Leu Tyr Thr Arg Trp Leu Lys Glu Ala Gly 500 505 510 Ala Thr Phe Ile Ile Thr Arg Pro Asp Phe Tyr Val Tyr Ser Thr Ala 515 520 525 Val Asp Ala Glu Gln Leu Gln Thr Gln Ile Lys Gln Leu Ser Asp Leu 530 535 540 Leu His Leu Asn Ser Val Val Gly Ala 545 550 <210> 3 <211> 679 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (649) <223> RXA00110 <400> 3 atatcgaatg tccagtcacg ctaaacttca cccaagatga gatttcgtgg cacgattggg 60 aataacgaac gctaatttta aacactggag gagcttccac gtg agc aac aaa gac 115 Val Ser Asn Lys Asp 1 5 ggc ctt ttt act gac ggt aac agc acg ttt gca cct aag gtg gat tca 163 Gly Leu Phe Thr Asp Gly Asn Ser Thr Phe Ala Pro Lys Val Asp Ser 10 15 20 att ccc ctc agc gat gtg gat acc agc gtt agc ggt gaa gcc tcc atc 211 Ile Pro Leu Ser Asp Val Asp Thr Ser Val Ser Gly Glu Ala Ser Ile 25 30 35 ggc acg ctg atc tcc aac gca acc tcc caa atg tcc agc ctt ttc cgc 259 Gly Thr Leu Ile Ser Asn Ala Thr Ser Gln Met Ser Ser Leu Phe Arg 40 45 50 gca gaa gtt gag ctg gcg aag act gaa ctc gca ggc gaa gcc aag aaa 307 Ala Glu Val Glu Leu Ala Lys Thr Glu Leu Ala Gly Glu Ala Lys Lys 55 60 65 gct gcc atc ggc ggc ggc gca ttc agc gtt gct ggc gta atc gca ctg 355 Ala Ala Ile Gly Gly Gly Ala Phe Ser Val Ala Gly Val Ile Ala Leu 70 75 80 85 tac agc tcc ttc ttc ttt ttc ttc ttc gtc gca gca ctg ctg agc gag 403 Tyr Ser Ser Phe Phe Phe Phe Phe Phe Val Ala Ala Leu Leu Ser Glu 90 95 100 tgg att aag cct tgg gca gca ttc ctc atc gtg ttc ctc ttc atg ctg 451 Trp Ile Lys Pro Trp Ala Ala Phe Leu Ile Val Phe Leu Phe Met Leu 105 110 115 gtc atc gcc gca gct ctc gca ctg ttc ggc tgg cgc aag gtg aag aag 499 Val Ile Ala Ala Ala Leu Ala Leu Phe Gly Trp Arg Lys Val Lys Lys 120 125 130 atg ggc gct ccg aag aac acc atc caa tcg gtc aac caa ctg aag aac 547 Met Gly Ala Pro Lys Asn Thr Ile Gln Ser Val Asn Gln Leu Lys Asn 135 140 145 ctg gtc cca ggt cag gca tcc gag aag ctg gag aag gcc aac aag cgt 595 Leu Val Pro Gly Gln Ala Ser Glu Lys Leu Glu Lys Ala Asn Lys Arg 150 155 160 165 ggc ctc tac acc tcc gcg tcc ttc cac agc ccc ggc gcc atc act ggc 643 Gly Leu Tyr Thr Ser Ala Ser Phe His Ser Pro Gly Ala Ile Thr Gly 170 175 180 gac cac taaaaaagga gacttcgatg gcctttttta 679 Asp his <210> 4 <211> 183 <212> PRT <213> Corynebacterium glutamicum <400> 4 Val Ser Asn Lys Asp Gly Leu Phe Thr Asp Gly Asn Ser Thr Phe Ala 1 5 10 15 Pro Lys Val Asp Ser Ile Pro Leu Ser Asp Val Asp Thr Ser Val Ser 20 25 30 Gly Glu Ala Ser Ile Gly Thr Leu Ile Ser Asn Ala Thr Ser Gln Met 35 40 45 Ser Ser Leu Phe Arg Ala Glu Val Glu Leu Ala Lys Thr Glu Leu Ala 50 55 60 Gly Glu Ala Lys Lys Ala Ala Ile Gly Gly Gly Ala Phe Ser Val Ala 65 70 75 80 Gly Val Ile Ala Leu Tyr Ser Ser Phe Phe Phe Phe Phe Phe Val Ala 85 90 95 Ala Leu Leu Ser Glu Trp Ile Lys Pro Trp Ala Ala Phe Leu Ile Val 100 105 110 Phe Leu Phe Met Leu Val Ile Ala Ala Ala Leu Ala Leu Phe Gly Trp 115 120 125 Arg Lys Val Lys Lys Met Gly Ala Pro Lys Asn Thr Ile Gln Ser Val 130 135 140 Asn Gln Leu Lys Asn Leu Val Pro Gly Gln Ala Ser Glu Lys Leu Glu 145 150 155 160 Lys Ala Asn Lys Arg Gly Leu Tyr Thr Ser Ala Ser Phe His Ser Pro 165 170 175 Gly Ala Ile Thr Gly Asp His 180 <210> 5 <211> 1213 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1183) <223> RXA00137 <400> 5 gagtgcttat gaggcaatca gccactaagt gttgagtaat ctactagttt ggactagaag 60 ttacccactt tcagtgaatt tttaaggaga gaacatggct ttg gct gat acc cga 115 Leu Ala Asp Thr Arg 1 5 ttt gcc act cgt cgt cgc gca ctt gcc gca aaa ctg gca gct caa cgg 163 Phe Ala Thr Arg Arg Arg Ala Leu Ala Ala Lys Leu Ala Ala Gln Arg 10 15 20 atc gac tca att ttg gtg aca agc ccg atc cat gtt cgc tat ctc agc 211 Ile Asp Ser Ile Leu Val Thr Ser Pro Ile His Val Arg Tyr Leu Ser 25 30 35 gga ttc acc ggc tcc aac ggc gca ctg atc gtg aac aaa gat ctc tcc 259 Gly Phe Thr Gly Ser Asn Gly Ala Leu Ile Val Asn Lys Asp Leu Ser 40 45 50 gcg cag atc tgc acc gac ggt cgc tac acc acc cag atc gca gaa gaa 307 Ala Gln Ile Cys Thr Asp Gly Arg Tyr Thr Thr Gln Ile Ala Glu Glu 55 60 65 gtc ccg gac atc gag gcg ctg att gag cgt gcc tcg gca acg acg ctg 355 Val Pro Asp Ile Glu Ala Leu Ile Glu Arg Ala Ser Ala Thr Thr Leu 70 75 80 85 cta gcg cag gtc gaa ggg ccg cgt cgt ata gca atc gaa gcc gca caa 403 Leu Ala Gln Val Glu Gly Pro Arg Arg Ile Ala Ile Glu Ala Ala Gln 90 95 100 acc acc ctg gac cag cta gac agc ctg cgt gaa gca acc cag gaa gac 451 Thr Thr Leu Asp Gln Leu Asp Ser Leu Arg Glu Ala Thr Gln Glu Asp 105 110 115 gtc gag ctg atc ccc gtg tca ggt gtt gtg gaa tcc att cgc ctg acc 499 Val Glu Leu Ile Pro Val Ser Gly Val Val Glu Ser Ile Arg Leu Thr 120 125 130 aaa gac agc ttc gaa ctc gac cgc ctc cgc gat gtc gca gcg ctg gct 547 Lys Asp Ser Phe Glu Leu Asp Arg Leu Arg Asp Val Ala Ala Leu Ala 135 140 145 tcc caa gca ttc gaa gat tta ctc gca gca gga gaa ctc gcc gaa ggc 595 Ser Gln Ala Phe Glu Asp Leu Leu Ala Ala Gly Glu Leu Ala Glu Gly 150 155 160 165 cga tca gag cgc caa gtc gcc gcc gat ctg gaa tac cgc atg cgc ctg 643 Arg Ser Glu Arg Gln Val Ala Ala Asp Leu Glu Tyr Arg Met Arg Leu 170 175 180 ttg gga gca gaa cgc ccc agc ttc gac acc atc gtg gcc tct gga cct 691 Leu Gly Ala Glu Arg Pro Ser Phe Asp Thr Ile Val Ala Ser Gly Pro 185 190 195 aac tcc gcg aaa cca cac cac ggc gca ggc gac cgc atc ctc cag cgc 739 Asn Ser Ala Lys Pro His His Gly Ala Gly Asp Arg Ile Leu Gln Arg 200 205 210 ggc gat cta gtc acc atc gat ttc ggc gca cac gca cgc gga ttc aac 787 Gly Asp Leu Val Thr Ile Asp Phe Gly Ala His Ala Arg Gly Phe Asn 215 220 225 tcc gat atg acc cgc acc ctc gtt atg ggc gaa gca ggg gag ttc gaa 835 Ser Asp Met Thr Arg Thr Leu Val Met Gly Glu Ala Gly Glu Phe Glu 230 235 240 245 gca gaa atc tac gac atc gtc ctg cgc tcc caa ctc gct ggt gtt gaa 883 Ala Glu Ile Tyr Asp Ile Val Leu Arg Ser Gln Leu Ala Gly Val Glu 250 255 260 gca gcc tac tca ggc gcc aac ctc ttc gac atc gac gca gca tgc cgc 931 Ala Ala Tyr Ser Gly Ala Asn Leu Phe Asp Ile Asp Ala Ala Cys Arg 265 270 275 aaa atc atc gaa gac gca ggc tac ggc gaa tac ttc gtg cac tcc acc 979 Lys Ile Ile Glu Asp Ala Gly Tyr Gly Glu Tyr Phe Val His Ser Thr 280 285 290 ggc cac ggc atc gga ctt gaa gtc cac gaa gcc cca agc gca tcc aaa 1027 Gly His Gly Ile Gly Leu Glu Val His Glu Ala Pro Ser Ala Ser Lys 295 300 305 acc tca caa gga gtc cta gaa acc ggc tcc aca ctg acc atc gaa ccc 1075 Thr Ser Gln Gly Val Leu Glu Thr Gly Ser Thr Leu Thr Ile Glu Pro 310 315 320 325 gga att tac gtc ccc gga aag ggc ggc gta cgc atc gaa gac acc ctg 1123 Gly Ile Tyr Val Pro Gly Lys Gly Gly Val Arg Ile Glu Asp Thr Leu 330 335 340 att att acc tca gga gca ccg gaa atc atc acc aag gtg agt aag gac 1171 Ile Ile Thr Ser Gly Ala Pro Glu Ile Ile Thr Lys Val Ser Lys Asp 345 350 355 ctc atc gtg gtg taatctaggt gagctaatcg gtctgcgcgt 1213 Leu Ile Val Val 360 <210> 6 <211> 361 <212> PRT <213> Corynebacterium glutamicum <400> 6 Leu Ala Asp Thr Arg Phe Ala Thr Arg Arg Arg Ala Leu Ala Ala Lys 1 5 10 15 Leu Ala Ala Gln Arg Ile Asp Ser Ile Leu Val Thr Ser Pro Ile His 20 25 30 Val Arg Tyr Leu Ser Gly Phe Thr Gly Ser Asn Gly Ala Leu Ile Val 35 40 45 Asn Lys Asp Leu Ser Ala Gln Ile Cys Thr Asp Gly Arg Tyr Thr Thr 50 55 60 Gln Ile Ala Glu Glu Val Pro Asp Ile Glu Ala Leu Ile Glu Arg Ala 65 70 75 80 Ser Ala Thr Thr Leu Leu Ala Gln Val Glu Gly Pro Arg Arg Ile Ala 85 90 95 Ile Glu Ala Ala Gln Thr Thr Leu Asp Gln Leu Asp Ser Leu Arg Glu 100 105 110 Ala Thr Gln Glu Asp Val Glu Leu Ile Pro Val Ser Gly Val Val Glu 115 120 125 Ser Ile Arg Leu Thr Lys Asp Ser Phe Glu Leu Asp Arg Leu Arg Asp 130 135 140 Val Ala Ala Leu Ala Ser Gln Ala Phe Glu Asp Leu Leu Ala Ala Gly 145 150 155 160 Glu Leu Ala Glu Gly Arg Ser Glu Arg Gln Val Ala Ala Asp Leu Glu 165 170 175 Tyr Arg Met Arg Leu Leu Gly Ala Glu Arg Pro Ser Phe Asp Thr Ile 180 185 190 Val Ala Ser Gly Pro Asn Ser Ala Lys Pro His His Gly Ala Gly Asp 195 200 205 Arg Ile Leu Gln Arg Gly Asp Leu Val Thr Ile Asp Phe Gly Ala His 210 215 220 Ala Arg Gly Phe Asn Ser Asp Met Thr Arg Thr Leu Val Met Gly Glu 225 230 235 240 Ala Gly Glu Phe Glu Ala Glu Ile Tyr Asp Ile Val Leu Arg Ser Gln 245 250 255 Leu Ala Gly Val Glu Ala Ala Tyr Ser Gly Ala Asn Leu Phe Asp Ile 260 265 270 Asp Ala Ala Cys Arg Lys Ile Ile Glu Asp Ala Gly Tyr Gly Glu Tyr 275 280 285 Phe Val His Ser Thr Gly His Gly Ile Gly Leu Glu Val His Glu Ala 290 295 300 Pro Ser Ala Ser Lys Thr Ser Gln Gly Val Leu Glu Thr Gly Ser Thr 305 310 315 320 Leu Thr Ile Glu Pro Gly Ile Tyr Val Pro Gly Lys Gly Gly Val Arg 325 330 335 Ile Glu Asp Thr Leu Ile Ile Thr Ser Gly Ala Pro Glu Ile Ile Thr 340 345 350 Lys Val Ser Lys Asp Leu Ile Val Val 355 360 <210> 7 <211> 1426 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1396) <223> RXA00152 <400> 7 gtcattgata tccaaggcac gaccgcgatt gtatggaaag aagcctaaat ttttaacaat 60 caaatagtac tggccattcc caactaaaac tggagtaacg atg aca gga cta atc 115 Met Thr Gly Leu Ile 1 5 ctc gcc ata gtt ttc ctg gtc ttt gtc gcc gtc gtg gtg atc aag tcc 163 Leu Ala Ile Val Phe Leu Val Phe Val Ala Val Val Val Ile Lys Ser 10 15 20 ata gcc ctg att ccc cag ggt gaa gcc gcc gtc att gaa cgc ctt ggt 211 Ile Ala Leu Ile Pro Gln Gly Glu Ala Ala Val Ile Glu Arg Leu Gly 25 30 35 agc tac acc cgc acc gtt tca ggt ggc ctg acc ctg ctg gtt cca ttc 259 Ser Tyr Thr Arg Thr Val Ser Gly Gly Leu Thr Leu Leu Val Pro Phe 40 45 50 gtg gac cga gta cgc gca agg atc gac acc cgt gag cgc gtg gtc tca 307 Val Asp Arg Val Arg Ala Arg Ile Asp Thr Arg Glu Arg Val Val Ser 55 60 65 ttc cca ccg cag gct gtt att acc caa gac aac ctg acc gtg gcc atc 355 Phe Pro Pro Gln Ala Val Ile Thr Gln Asp Asn Leu Thr Val Ala Ile 70 75 80 85 gat atc gtg gtg acc ttc caa atc aac gaa cca gag cgc gcc atc tac 403 Asp Ile Val Val Thr Phe Gln Ile Asn Glu Pro Glu Arg Ala Ile Tyr 90 95 100 ggc gtg gac aac tac atc gtc ggt gtg gag cag att tct gta gca aca 451 Gly Val Asp Asn Tyr Ile Val Gly Val Glu Gln Ile Ser Val Ala Thr 105 110 115 ctt cga gac gtt gtc ggt ggc atg acc ctg gaa gaa acc ctc act tca 499 Leu Arg Asp Val Val Gly Gly Met Thr Leu Glu Glu Thr Leu Thr Ser 120 125 130 cgt gac gtg atc aac cgc cgc ctc cgt ggc gag ctc gat gca gca acc 547 Arg Asp Val Ile Asn Arg Arg Leu Arg Gly Glu Leu Asp Ala Ala Thr 135 140 145 acc aaa tgg ggc ctg cgc atc agc cgt gtg gaa cta aag gca att gat 595 Thr Lys Trp Gly Leu Arg Ile Ser Arg Val Glu Leu Lys Ala Ile Asp 150 155 160 165 ccg cca cca tcc atc cag caa tcg atg gaa aag cag atg aag gca gac 643 Pro Pro Pro Ser Ile Gln Gln Ser Met Glu Lys Gln Met Lys Ala Asp 170 175 180 cgt gaa aag cgc gcc acc att ttg acc gca gaa ggt cag cgc gaa gcc 691 Arg Glu Lys Arg Ala Thr Ile Leu Thr Ala Glu Gly Gln Arg Glu Ala 185 190 195 gac atc aaa act gcc gaa ggt gaa aag caa gcc aag atc ctc caa gct 739 Asp Ile Lys Thr Ala Glu Gly Glu Lys Gln Ala Lys Ile Leu Gln Ala 200 205 210 gag ggt gaa aag cac gca tcc atc ctg aac gca gaa gca gaa cgc caa 787 Glu Gly Glu Lys His Ala Ser Ile Leu Asn Ala Glu Ala Glu Arg Gln 215 220 225 gcg atg atc ctg cgc gcc gaa ggt gaa cgc gca gca cgc tac ctc cag 835 Ala Met Ile Leu Arg Ala Glu Gly Glu Arg Ala Ala Arg Tyr Leu Gln 230 235 240 245 gcg cag ggt gaa gcc cga gca atc caa aag gtc aac gca gca atc aag 883 Ala Gln Gly Glu Ala Arg Ala Ile Gln Lys Val Asn Ala Ala Ile Lys 250 255 260 tct gcc aag ttg acc cca gag gtt ctt gct tat caa tac ctc gaa aag 931 Ser Ala Lys Leu Thr Pro Glu Val Leu Ala Tyr Gln Tyr Leu Glu Lys 265 270 275 ctt cct aag atc gca gag ggc aac gcc tcc aag atg tgg gtc atc cca 979 Leu Pro Lys Ile Ala Glu Gly Asn Ala Ser Lys Met Trp Val Ile Pro 280 285 290 agc cag ttc tcc gat tct ctg gaa ggt ttt gcg aag cag ttc ggc gca 1027 Ser Gln Phe Ser Asp Ser Leu Glu Gly Phe Ala Lys Gln Phe Gly Ala 295 300 305 aag gat gca gaa ggt gtc ttc cgc tac gaa cca aac acc gtg gat gaa 1075 Lys Asp Ala Glu Gly Val Phe Arg Tyr Glu Pro Asn Thr Val Asp Glu 310 315 320 325 gaa acc cgc gac atc gca aac gcc gac aac gtg gaa gac tgg ttc tcc 1123 Glu Thr Arg Asp Ile Ala Asn Ala Asp Asn Val Glu Asp Trp Phe Ser 330 335 340 acc gaa tca gac cct gaa atc gca gca gca gtc gcc gca gca aac gcc 1171 Thr Glu Ser Asp Pro Glu Ile Ala Ala Ala Val Ala Ala Ala Asn Ala 345 350 355 gtg gcc aac aag cca gtc gat cca gaa ccc ggt gag atc ctt tcc aag 1219 Val Ala Asn Lys Pro Val Asp Pro Glu Pro Gly Glu Ile Leu Ser Lys 360 365 370 aag acc gca cga cgc gtt gaa cct gaa gca gta ttg gag gct ttg caa 1267 Lys Thr Ala Arg Arg Val Glu Pro Glu Ala Val Leu Glu Ala Leu Gln 375 380 385 aac gga acc act aca caa cct gag gtt gag gca gca cct cct acc gca 1315 Asn Gly Thr Thr Thr Gln Pro Glu Val Glu Ala Ala Pro Pro Thr Ala 390 395 400 405 aac ttc gcc caa gaa ttc cct gca cca cag gca aac cct gaa gat tac 1363 Asn Phe Ala Gln Glu Phe Pro Ala Pro Gln Ala Asn Pro Glu Asp Tyr 410 415 420 tcc gac caa cac cga gag aat cct tac gga aac taatcaggca taagaaaagg 1416 Ser Asp Gln His Arg Glu Asn Pro Tyr Gly Asn 425 430 cgggaaacac 1426 <210> 8 <211> 432 <212> PRT <213> Corynebacterium glutamicum <400> 8 Met Thr Gly Leu Ile Leu Ala Ile Val Phe Leu Val Phe Val Ala Val 1 5 10 15 Val Val Ile Lys Ser Ile Ala Leu Ile Pro Gln Gly Glu Ala Ala Val 20 25 30 Ile Glu Arg Leu Gly Ser Tyr Thr Arg Thr Val Ser Gly Gly Leu Thr 35 40 45 Leu Leu Val Pro Phe Val Asp Arg Val Arg Ala Arg Ile Asp Thr Arg 50 55 60 Glu Arg Val Val Ser Phe Pro Pro Gln Ala Val Ile Thr Gln Asp Asn 65 70 75 80 Leu Thr Val Ala Ile Asp Ile Val Val Thr Phe Gln Ile Asn Glu Pro 85 90 95 Glu Arg Ala Ile Tyr Gly Val Asp Asn Tyr Ile Val Gly Val Glu Gln 100 105 110 Ile Ser Val Ala Thr Leu Arg Asp Val Val Gly Gly Met Thr Leu Glu 115 120 125 Glu Thr Leu Thr Ser Arg Asp Val Ile Asn Arg Arg Leu Arg Gly Glu 130 135 140 Leu Asp Ala Ala Thr Thr Lys Trp Gly Leu Arg Ile Ser Arg Val Glu 145 150 155 160 Leu Lys Ala Ile Asp Pro Pro Pro Ile Gln Gln Ser Met Glu Lys 165 170 175 Gln Met Lys Ala Asp Arg Glu Lys Arg Ala Thr Ile Leu Thr Ala Glu 180 185 190 Gly Gln Arg Glu Ala Asp Ile Lys Thr Ala Glu Gly Glu Lys Gln Ala 195 200 205 Lys Ile Leu Gln Ala Glu Gly Glu Lys His Ala Ser Ile Leu Asn Ala 210 215 220 Glu Ala Glu Arg Gln Ala Met Ile Leu Arg Ala Glu Gly Glu Arg Ala 225 230 235 240 Ala Arg Tyr Leu Gln Ala Gln Gly Glu Ala Arg Ala Ile Gln Lys Val 245 250 255 Asn Ala Ala Ile Lys Ser Ala Lys Leu Thr Pro Glu Val Leu Ala Tyr 260 265 270 Gln Tyr Leu Glu Lys Leu Pro Lys Ile Ala Glu Gly Asn Ala Ser Lys 275 280 285 Met Trp Val Ile Pro Ser Gln Phe Ser Asp Ser Leu Glu Gly Phe Ala 290 295 300 Lys Gln Phe Gly Ala Lys Asp Ala Glu Gly Val Phe Arg Tyr Glu Pro 305 310 315 320 Asn Thr Val Asp Glu Glu Thr Arg Asp Ile Ala Asn Ala Asp Asn Val 325 330 335 Glu Asp Trp Phe Ser Thr Glu Ser Asp Pro Glu Ile Ala Ala Ala Val 340 345 350 Ala Ala Ala Asn Ala Val Ala Asn Lys Pro Val Asp Pro Glu Pro Gly 355 360 365 Glu Ile Leu Ser Lys Lys Thr Ala Arg Arg Val Glu Pro Glu Ala Val 370 375 380 Leu Glu Ala Leu Gln Asn Gly Thr Thr Thr Thr Gln Pro Glu Val Glu Ala 385 390 395 400 Ala Pro Pro Thr Ala Asn Phe Ala Gln Glu Phe Pro Ala Pro Gln Ala 405 410 415 Asn Pro Glu Asp Tyr Ser Asp Gln His Arg Glu Asn Pro Tyr Gly Asn 420 425 430 <210> 9 <211> 1048 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (1018) <223> RXA00166 <400> 9 tgcgacaacg ccactttgag agtccataat ttggcatcca acgccggcat cttggacggt 60 cgcgcgccgg gcccggggag gtttttatac ctatctaaag gtg tgg att tcg ttt 115 Val Trp Ile Ser Phe 1 5 gtt gca caa gga gat act gta tct cat gag cac gtt aag cga tat atc 163 Val Ala Gln Gly Asp Thr Val Ser His Glu His Val Lys Arg Tyr Ile 10 15 20 gct agt gaa ggt ggt ggt agc ccg cta gga tta aca tct gat atg aat 211 Ala Ser Glu Gly Gly Gly Ser Pro Leu Gly Leu Thr Ser Asp Met Asn 25 30 35 gat cca gct cat ccc cca att cca cca gta tca aaa cga gac gct ccg 259 Asp Pro Ala His Pro Pro Ile Pro Pro Val Ser Lys Arg Asp Ala Pro 40 45 50 gcg ttt agc gat ctt caa cat cga gca acc agc gcc agc gct ttt acc 307 Ala Phe Ser Asp Leu Gln His Arg Ala Thr Ser Ala Ser Ala Phe Thr 55 60 65 caa ggc agc acg act tat cac gat gtc cga cct gga tat ccg gct gag 355 Gln Gly Ser Thr Thr Tyr His Asp Val Arg Pro Gly Tyr Pro Ala Glu 70 75 80 85 gcc gtg gag tta gcc cgt ggg ttt ggc cga gtc ctg gat gtc ggt gca 403 Ala Val Glu Leu Ala Arg Gly Phe Gly Arg Val Leu Asp Val Gly Ala 90 95 100 ggt acc gga aaa cta acc agt gag cta aca gct gat cag gtc cta gcc 451 Gly Thr Gly Lys Leu Thr Ser Glu Leu Thr Ala Asp Gln Val Leu Ala 105 110 115 ctt gat cca agc atg gac atg ttg cgg gtg ttt cgc tcc gcg ctt ccg 499 Leu Asp Pro Ser Met Asp Met Leu Arg Val Phe Arg Ser Ala Leu Pro 120 125 130 gcg gtt ccc tgc tgg caa gcg aca gca gaa cac aca gga ata cgt gac 547 Ala Val Pro Cys Trp Gln Ala Thr Ala Glu His Thr Gly Ile Arg Asp 135 140 145 aac gcg gtt gat ctg att acg tgc gca caa acg tgg cat tgg gtt gac 595 Asn Ala Val Asp Leu Ile Thr Cys Ala Gln Thr Trp His Trp Val Asp 150 155 160 165 gtg acg gct gcc tca gcg gaa ttt gat cgg gtg att gca cct gag ggt 643 Val Thr Ala Ala Ser Ala Glu Phe Asp Arg Val Ile Ala Pro Glu Gly 170 175 180 gca gtc ctg ctc gtg tgg aat aac ctg gac acc tcc atc gcg tgg gta 691 Ala Val Leu Leu Val Trp Asn Asn Leu Asp Thr Ser Ile Ala Trp Val 185 190 195 cac cga ctc agt cgc att atg cat gcc ggc gat gta ctc aag ccg gga 739 His Arg Leu Ser Arg Ile Met His Ala Gly Asp Val Leu Lys Pro Gly 200 205 210 ttc acc cca gaa acc gca gct ccc tgg ata att gat cga gaa att cgc 787 Phe Thr Pro Glu Thr Ala Ala Pro Trp Ile Ile Asp Arg Glu Ile Arg 215 220 225 acc acg tgg aat cag cac ctc acc cct gaa gaa atc atc cag ctc gct 835 Thr Thr Trp Asn Gln His Leu Thr Pro Glu Glu Ile Ile Gln Leu Ala 230 235 240 245 cac acg agg tcc tac tgg tta aac gcg tca gag aaa atc aaa gag cgt 883 His Thr Arg Ser Tyr Trp Leu Asn Ala Ser Glu Lys Ile Lys Glu Arg 250 255 260 gtt gat cag aac ctt cag tgg tat ctc tac gag cat ttg ggt ttc agt 931 Val Asp Gln Asn Leu Gln Trp Tyr Leu Tyr Glu His Leu Gly Phe Ser 265 270 275 ccc gac aat cca gtg gaa ctt ccc tat cgc tgt gat gca ttt tta ctt 979 Pro Asp Asn Pro Val Glu Leu Pro Tyr Arg Cys Asp Ala Phe Leu Leu 280 285 290 tca cgt tcc ggt acc ctg gca ggc aga tct tcc aat ctt taggagccct 1028 Ser Arg Ser Gly Thr Leu Ala Gly Arg Ser Ser Asn Leu 295 300 305 cgccatgtac ctgttgaatc 1048 <210> 10 <211> 306 <212> PRT <213> Corynebacterium glutamicum <400> 10 Val Trp Ile Ser Phe Val Ala Gln Gly Asp Thr Val Ser His Glu His 1 5 10 15 Val Lys Arg Tyr Ile Ala Ser Glu Gly Gly Gly Ser Pro Leu Gly Leu 20 25 30 Thr Ser Asp Met Asn Asp Pro Ala His Pro Pro Ile Pro Pro Val Ser 35 40 45 Lys Arg Asp Ala Pro Ala Phe Ser Asp Leu Gln His Arg Ala Thr Ser 50 55 60 Ala Ser Ala Phe Thr Gln Gly Ser Thr Thr Tyr His Asp Val Arg Pro 65 70 75 80 Gly Tyr Pro Ala Glu Ala Val Glu Leu Ala Arg Gly Phe Gly Arg Val 85 90 95 Leu Asp Val Gly Ala Gly Thr Gly Lys Leu Thr Ser Glu Leu Thr Ala 100 105 110 Asp Gln Val Leu Ala Leu Asp Pro Ser Met Asp Met Leu Arg Val Phe 115 120 125 Arg Ser Ala Leu Pro Ala Val Pro Cys Trp Gln Ala Thr Ala Glu His 130 135 140 Thr Gly Ile Arg Asp Asn Ala Val Asp Leu Ile Thr Cys Ala Gln Thr 145 150 155 160 Trp His Trp Val Asp Val Thr Ala Ala Ser Ala Glu Phe Asp Arg Val 165 170 175 Ile Ala Pro Glu Gly Ala Val Leu Leu Val Trp Asn Asn Leu Asp Thr 180 185 190 Ser Ile Ala Trp Val His Arg Leu Ser Arg Ile Met His Ala Gly Asp 195 200 205 Val Leu Lys Pro Gly Phe Thr Pro Glu Thr Ala Ala Pro Trp Ile Ile 210 215 220 Asp Arg Glu Ile Arg Thr Thr Trp Asn Gln His Leu Thr Pro Glu Glu 225 230 235 240 Ile Ile Gln Leu Ala His Thr Arg Ser Tyr Trp Leu Asn Ala Ser Glu 245 250 255 Lys Ile Lys Glu Arg Val Asp Gln Asn Leu Gln Trp Tyr Leu Tyr Glu 260 265 270 His Leu Gly Phe Ser Pro Asp Asn Pro Val Glu Leu Pro Tyr Arg Cys 275 280 285 Asp Ala Phe Leu Leu Ser Arg Ser Gly Thr Leu Ala Gly Arg Ser Ser 290 295 300 Asn leu 305 <210> 11 <211> 1342 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (101) .. (1312) <223> RXA00197 <400> 11 gtcgatgata ttttggcaac cgaatctgag gcacgcgcgc gtgcgaatgc tttgatcaac 60 cggttggcaa ccaacttgta agctaaggag cttccgcctc gtg gca gcc tat ctt 115 Val Ala Ala Tyr Leu 1 5 ctt ggt gtc gta tta ttt ttc ctc ggc atc gca gta acc atc gcg ctt 163 Leu Gly Val Val Leu Phe Phe Leu Gly Ile Ala Val Thr Ile Ala Leu 10 15 20 cac gag tgg ggg cac ttc atc aca gcg cgc att ttc gga atg aaa gtg 211 His Glu Trp Gly His Phe Ile Thr Ala Arg Ile Phe Gly Met Lys Val 25 30 35 cgg cgt ttc ttc atc ggt ttc ggc ccg acg gtg ttt gcc aaa aga cgc 259 Arg Arg Phe Phe Ile Gly Phe Gly Pro Thr Val Phe Ala Lys Arg Arg 40 45 50 ggc gaa acc gtg tac ggc ctt aaa gcg att ccg gtc ggc ggt ttt tgt 307 Gly Glu Thr Val Tyr Gly Leu Lys Ala Ile Pro Val Gly Gly Phe Cys 55 60 65 gac atc gcg ggg atg act gcc caa gat gaa ctt gat ccg gaa gac ctg 355 Asp Ile Ala Gly Met Thr Ala Gln Asp Glu Leu Asp Pro Glu Asp Leu 70 75 80 85 ccg cgc gcc atg tat cta aag ccc tgg tgg cag cgc ata att gtg ctt 403 Pro Arg Ala Met Tyr Leu Lys Pro Trp Trp Gln Arg Ile Ile Val Leu 90 95 100 tcc ggc ggc gtg atc atg aat ctg atc gtc ggc ttt ttg gtg ctt tac 451 Ser Gly Gly Val Ile Met Asn Leu Ile Val Gly Phe Leu Val Leu Tyr 105 110 115 ggc gtg gcg gtg agc tcc gga atc ccg aat ccg gat gtg gat acc acc 499 Gly Val Ala Val Ser Ser Gly Ile Pro Asn Pro Asp Val Asp Thr Thr 120 125 130 gcg aca gtc gac acc gtt cag tgc gtg ccg gaa acc caa att tcc gca 547 Ala Thr Val Asp Thr Val Gln Cys Val Pro Glu Thr Gln Ile Ser Ala 135 140 145 act gaa ctg tcc tcc tgc gta ggt tca ggc cca gcg ggc gac gcc ggc 595 Thr Glu Leu Ser Ser Cys Val Gly Ser Gly Pro Ala Gly Asp Ala Gly 150 155 160 165 att gag cac ggc gat aag att ttg gcc gtc aac ggc caa gag atg gca 643 Ile Glu His Gly Asp Lys Ile Leu Ala Val Asn Gly Gln Glu Met Ala 170 175 180 agc ttc acc gcc atc cgc gat gcg atc ctc gag ctc cca ggc gaa acg 691 Ser Phe Thr Ala Ile Arg Asp Ala Ile Leu Glu Leu Pro Gly Glu Thr 185 190 195 gca acg ctg acg att gaa cgg gag gga acg ctt ttc gac gtc gac ctc 739 Ala Thr Leu Thr Ile Glu Arg Glu Gly Thr Leu Phe Asp Val Asp Leu 200 205 210 cag gtt gcc tct gtc acc cgt ctc gcc tct gac ggt tca gaa att acc 787 Gln Val Ala Ser Val Thr Arg Leu Ala Ser Asp Gly Ser Glu Ile Thr 215 220 225 gtc ggc gcg gtg ggc atg tcg agc ctt cca ccg acc gat gtg tac aaa 835 Val Gly Ala Val Gly Met Ser Ser Leu Pro Pro Thr Asp Val Tyr Lys 230 235 240 245 aaa tac ggc cca atc gag ggt gtg gga gca act gca cgt ttc acc ggc 883 Lys Tyr Gly Pro Ile Glu Gly Val Gly Ala Thr Ala Arg Phe Thr Gly 250 255 260 gac atg atc agc gcc acg tgg gat ggc ctc aaa gcc ttc ccg gcg aaa 931 Asp Met Ile Ser Ala Thr Trp Asp Gly Leu Lys Ala Phe Pro Ala Lys 265 270 275 atc cca ggg gtc gtc gca tcc atc ttc ggt gca gaa cga gat gta gaa 979 Ile Pro Gly Val Val Ala Ser Ile Phe Gly Ala Glu Arg Asp Val Glu 280 285 290 agc ccc atg agt gtg gtg ggc gcg tca cgc atc ggc ggc gaa ttt gtc 1027 Ser Pro Met Ser Val Val Gly Ala Ser Arg Ile Gly Gly Glu Phe Val 295 300 305 gaa cgt tcc atg tgg gac atg ttc atg atg atg ctg gcc agc ctg aac 1075 Glu Arg Ser Met Trp Asp Met Phe Met Met Met Leu Ala Ser Leu Asn 310 315 320 325 ttc ttc ctc gcg ctg ttt aac ctc gtg ccg ctg cca cca ctt gat ggc 1123 Phe Phe Leu Ala Leu Phe Asn Leu Val Pro Leu Pro Pro Leu Asp Gly 330 335 340 gga cac att gcc gtg gtg atc tat gaa aaa atc cgc gac ttc ttc cgc 1171 Gly His Ile Ala Val Val Ile Tyr Glu Lys Ile Arg Asp Phe Phe Arg 345 350 355 aaa ctg cgc gga aaa cca gcg ggc ggc cca gcg gat tac acc aaa cta 1219 Lys Leu Arg Gly Lys Pro Ala Gly Gly Pro Ala Asp Tyr Thr Lys Leu 360 365 370 atg ccc gtc acc gta gct gtc gca gcc ttg ctg atg aca gtg gga ggc 1267 Met Pro Val Thr Val Ala Val Ala Ala Leu Leu Met Thr Val Gly Gly 375 380 385 ctg gtc att gtc gcc gat gtg gtc aat ccc atc cga ctc ttt ggc 1312 Leu Val Ile Val Ala Asp Val Val Asn Pro Ile Arg Leu Phe Gly 390 395 400 taacgatacg gaattgaact gccggcaccg 1342 <210> 12 <211> 404 <212> PRT <213> Corynebacterium glutamicum <400> 12 Val Ala Ala Tyr Leu Leu Gly Val Val Leu Phe Phe Leu Gly Ile Ala 1 5 10 15 Val Thr Ile Ala Leu His Glu Trp Gly His Phe Ile Thr Ala Arg Ile 20 25 30 Phe Gly Met Lys Val Arg Arg Phe Phe Ile Gly Phe Gly Pro Thr Val 35 40 45 Phe Ala Lys Arg Arg Gly Glu Thr Val Tyr Gly Leu Lys Ala Ile Pro 50 55 60 Val Gly Gly Phe Cys Asp Ile Ala Gly Met Thr Ala Gln Asp Glu Leu 65 70 75 80 Asp Pro Glu Asp Leu Pro Arg Ala Met Tyr Leu Lys Pro Trp Trp Gln 85 90 95 Arg Ile Ile Val Leu Ser Gly Gly Val Ile Met Asn Leu Ile Val Gly 100 105 110 Phe Leu Val Leu Tyr Gly Val Ala Val Ser Ser Gly Ile Pro Asn Pro 115 120 125 Asp Val Asp Thr Thr Ala Thr Val Asp Thr Val Gln Cys Val Pro Glu 130 135 140 Thr Gln Ile Ser Ala Thr Glu Leu Ser Ser Cys Val Gly Ser Gly Pro 145 150 155 160 Ala Gly Asp Ala Gly Ile Glu His Gly Asp Lys Ile Leu Ala Val Asn 165 170 175 Gly Gln Glu Met Ala Ser Phe Thr Ala Ile Arg Asp Ala Ile Leu Glu 180 185 190 Leu Pro Gly Glu Thr Ala Thr Leu Thr Ile Glu Arg Glu Gly Thr Leu 195 200 205 Phe Asp Val Asp Leu Gln Val Ala Ser Val Thr Arg Leu Ala Ser Asp 210 215 220 Gly Ser Glu Ile Thr Val Gly Ala Val Gly Met Ser Ser Leu Pro Pro 225 230 235 240 Thr Asp Val Tyr Lys Lys Tyr Gly Pro Ile Glu Gly Val Gly Ala Thr 245 250 255 Ala Arg Phe Thr Gly Asp Met Ile Ser Ala Thr Trp Asp Gly Leu Lys 260 265 270 Ala Phe Pro Ala Lys Ile Pro Gly Val Val Ala Ser Ile Phe Gly Ala 275 280 285 Glu Arg Asp Val Glu Ser Pro Met Ser Val Val Gly Ala Ser Arg Ile 290 295 300 Gly Gly Glu Phe Val Glu Arg Ser Met Trp Asp Met Phe Met Met Met 305 310 315 320 Leu Ala Ser Leu Asn Phe Phe Leu Ala Leu Phe Asn Leu Val Pro Leu 325 330 335 Pro Pro Leu Asp Gly Gly His Ile Ala Val Val Ile Tyr Glu Lys Ile 340 345 350 Arg Asp Phe Phe Arg Lys Leu Arg Gly Lys Pro Ala Gly Gly Pro Ala 355 360 365 Asp Tyr Thr Lys Leu Met Pro Val Thr Val Ala Val Ala Ala Leu Leu 370 375 380 Met Thr Val Gly Gly Leu Val Ile Val Ala Asp Val Val Asn Pro Ile 385 390 395 400 Arg Leu Phe Gly <210> 13 <211> 1036 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1006) <223> RXA00332 <400> 13 aatgaactct ggaaccgcca tgcagcaaaa cctctccaat tggtaatctt tgactcccag 60 gttacgccag ccctgcgaca ccaccatcta gggttagagt atg gcc ttc aac aaa 115 Met Ala Phe Asn Lys 1 5 gcg tac gat gca ctt cgc gcc cct caa atc acc ctc gga ctc atg aca 163 Ala Tyr Asp Ala Leu Arg Ala Pro Gln Ile Thr Leu Gly Leu Met Thr 10 15 20 cca aac ggc cct gaa cta ggg cgc agt gaa atg gtt cca acc gaa aat 211 Pro Asn Gly Pro Glu Leu Gly Arg Ser Glu Met Val Pro Thr Glu Asn 25 30 35 agc atc gaa cta gcc ata caa gca gaa gct caa gga ttc aga ggc atg 259 Ser Ile Glu Leu Ala Ile Gln Ala Glu Ala Gln Gly Phe Arg Gly Met 40 45 50 tgg gtt cga gac gtt cca ctc gca gtt cct caa gga atc act gtt acc 307 Trp Val Arg Asp Val Pro Leu Ala Val Pro Gln Gly Ile Thr Val Thr 55 60 65 gat aaa cag gct acg tat tta gat gat cca ttc tta atg ctc ggt gcg 355 Asp Lys Gln Ala Thr Tyr Leu Asp Asp Pro Phe Leu Met Leu Gly Ala 70 75 80 85 atg gcc tct gtg acc tct aca atc gcg ctg ggc act gca gcg acc gtg 403 Met Ala Ser Val Thr Ser Thr Ile Ala Leu Gly Thr Ala Ala Thr Val 90 95 100 ctt cca ctc aga cat ccg cta cat gtg gcg aaa tcc gcg ctc acc ctt 451 Leu Pro Leu Arg His Pro Leu His Val Ala Lys Ser Ala Leu Thr Leu 105 110 115 gat cga ctc agc cac gga cgt ttc gtt tta ggc atc ggc tct ggc gac 499 Asp Arg Leu Ser His Gly Arg Phe Val Leu Gly Ile Gly Ser Gly Asp 120 125 130 agg cct gaa gaa ttc gag att ttt ggc aaa agc tta gac aat cga cgc 547 Arg Pro Glu Glu Phe Glu Ile Phe Gly Lys Ser Leu Asp Asn Arg Arg 135 140 145 gct gat att cag tct ggg tgg gca att ttg cgt gca gct ttg tcg ccg 595 Ala Asp Ile Gln Ser Gly Trp Ala Ile Leu Arg Ala Ala Leu Ser Pro 150 155 160 165 gat cct gcg atg cgg gcc gac ctt gaa ttt gcg cca acc acg cca cct 643 Asp Pro Ala Met Arg Ala Asp Leu Glu Phe Ala Pro Thr Thr Pro Pro 170 175 180 gaa gct cag atc ccc atg atc gct gta ggt tct gcc cga caa aca gtg 691 Glu Ala Gln Ile Pro Met Ile Ala Val Gly Ser Ala Arg Gln Thr Val 185 190 195 caa tgg atc gcc cga aac gcc gac gga tgg gca acc tac tac cgc ccc 739 Gln Trp Ile Ala Arg Asn Ala Asp Gly Trp Ala Thr Tyr Tyr Arg Pro 200 205 210 gct gaa gct caa gtc gga cgc ctc gat ctc tgg gac aaa gcc cgt ggt 787 Ala Glu Ala Gln Val Gly Arg Leu Asp Leu Trp Asp Lys Ala Arg Gly 215 220 225 ggc acc cgc ccc ttg ttg att tcc tcc atg ggg ctc aac ctc acc gaa 835 Gly Thr Arg Pro Leu Leu Ile Ser Ser Met Gly Leu Asn Leu Thr Glu 230 235 240 245 gga aac acc cac aaa gaa atc act ctg ggc gta gag gta ggc agc cag 883 Gly Asn Thr His Lys Glu Ile Thr Leu Gly Val Glu Val Gly Ser Gln 250 255 260 gaa ctc atc gaa cac ctc cac cgc ctt tcg gaa ctt ggc atc gac cac 931 Glu Leu Ile Glu His Leu His Arg Leu Ser Glu Leu Gly Ile Asp His 265 270 275 gtc atc ttc aac atc cag cgc cga cca gca agc gaa gta ctg acc cag 979 Val Ile Phe Asn Ile Gln Arg Arg Pro Ala Ser Glu Val Leu Thr Gln 280 285 290 att tac gaa gaa gtg ctc ccc cac ctc taaaatgcct gttccggcac 1026 Ile Tyr Glu Glu Val Leu Pro His Leu 295 300 ttcccttcgg 1036 <210> 14 <211> 302 <212> PRT <213> Corynebacterium glutamicum <400> 14 Met Ala Phe Asn Lys Ala Tyr Asp Ala Leu Arg Ala Pro Gln Ile Thr 1 5 10 15 Leu Gly Leu Met Thr Pro Asn Gly Pro Glu Leu Gly Arg Ser Glu Met 20 25 30 Val Pro Thr Glu Asn Ser Ile Glu Leu Ala Ile Gln Ala Glu Ala Gln 35 40 45 Gly Phe Arg Gly Met Trp Val Arg Asp Val Pro Leu Ala Val Pro Gln 50 55 60 Gly Ile Thr Val Thr Asp Lys Gln Ala Thr Tyr Leu Asp Asp Pro Phe 65 70 75 80 Leu Met Leu Gly Ala Met Ala Ser Val Thr Ser Thr Ile Ala Leu Gly 85 90 95 Thr Ala Ala Thr Val Leu Pro Leu Arg His Pro Leu His Val Ala Lys 100 105 110 Ser Ala Leu Thr Leu Asp Arg Leu Ser His Gly Arg Phe Val Leu Gly 115 120 125 Ile Gly Ser Gly Asp Arg Pro Glu Glu Phe Glu Ile Phe Gly Lys Ser 130 135 140 Leu Asp Asn Arg Arg Ala Asp Ile Gln Ser Gly Trp Ala Ile Leu Arg 145 150 155 160 Ala Ala Leu Ser Pro Asp Pro Ala Met Arg Ala Asp Leu Glu Phe Ala 165 170 175 Pro Thr Thr Pro Pro Glu Ala Gln Ile Pro Met Ile Ala Val Gly Ser 180 185 190 Ala Arg Gln Thr Val Gln Trp Ile Ala Arg Asn Ala Asp Gly Trp Ala 195 200 205 Thr Tyr Tyr Arg Pro Ala Glu Ala Gln Val Gly Arg Leu Asp Leu Trp 210 215 220 Asp Lys Ala Arg Gly Gly Thr Arg Pro Leu Leu Ile Ser Ser Met Gly 225 230 235 240 Leu Asn Leu Thr Glu Gly Asn Thr His Lys Glu Ile Thr Leu Gly Val 245 250 255 Glu Val Gly Ser Gln Glu Leu Ile Glu His Leu His Arg Leu Ser Glu 260 265 270 Leu Gly Ile Asp His Val Ile Phe Asn Ile Gln Arg Arg Pro Ala Ser 275 280 285 Glu Val Leu Thr Gln Ile Tyr Glu Glu Val Leu Pro His Leu 290 295 300 <210> 15 <211> 931 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (901) <223> RXA00397 <400> 15 tttttcgagg ttggggtggc ttttcgggtg gcgtgtatgt attaaaccaa ggagcttgtt 60 tgagggggag cgcttggttt gattcgacag ttttcgggca ttg agc ctg acg gaa 115 Leu Ser Leu Thr Glu 1 5 ggg gag atc att ccc atc caa aca aag ctc ggc cac tgg cgt gac gtg 163 Gly Glu Ile Ile Pro Ile Gln Thr Lys Leu Gly His Trp Arg Asp Val 10 15 20 cgg gat tcc gaa gat ctg cca ttc caa gta atc gca tcg cgc acc agc 211 Arg Asp Ser Glu Asp Leu Pro Phe Gln Val Ile Ala Ser Arg Thr Ser 25 30 35 gtg acc att atg gaa aac acc ctg gaa tgg acc aac agc cgc ctt gag 259 Val Thr Ile Met Glu Asn Thr Leu Glu Trp Thr Asn Ser Arg Leu Glu 40 45 50 cgt ggc gaa gag ttc acc gag ctc gac att tat gct cac tac ttg gaa 307 Arg Gly Glu Glu Phe Thr Glu Leu Asp Ile Tyr Ala His Tyr Leu Glu 55 60 65 gag ttg gaa gat tac gcc ctc caa ttc acc ttc gat gtc gag ccc tac 355 Glu Leu Glu Asp Tyr Ala Leu Gln Phe Thr Phe Asp Val Glu Pro Tyr 70 75 80 85 ttc acc aac ctg cac tcc tcc aac aga atg ctc ttc cct gga cct cct 403 Phe Thr Asn Leu His Ser Ser Asn Arg Met Leu Phe Pro Gly Pro Pro 90 95 100 gtg gat ttc ccg atc aac gca gaa acc cga tgc att cag ctc gac gcc 451 Val Asp Phe Pro Ile Asn Ala Glu Thr Arg Cys Ile Gln Leu Asp Ala 105 110 115 ggt gtt gca gta aag aag gac ggc gtg gtg ctg ggt acc tca gat atg 499 Gly Val Ala Val Lys Lys Asp Gly Val Val Leu Gly Thr Ser Asp Met 120 125 130 gcg agg tcc ctg cct cga acc gcc gct ggc caa gaa gcc tat gag tac 547 Ala Arg Ser Leu Pro Arg Thr Ala Ala Gly Gln Glu Ala Tyr Glu Tyr 135 140 145 ttc ttc aag gtg gtt cgt gaa ggc atc atc ggg cag ctg cgc ccg ggc 595 Phe Phe Lys Val Val Arg Glu Gly Ile Ile Gly Gln Leu Arg Pro Gly 150 155 160 165 gtg atc tgc gct gac gtg cac gaa gca acc ctt gat tac cta agc ccg 643 Val Ile Cys Ala Asp Val His Glu Ala Thr Leu Asp Tyr Leu Ser Pro 170 175 180 cag cta cct cgc atg att gac atc gga atg ctg ggt gcc gac acc gat 691 Gln Leu Pro Arg Met Ile Asp Ile Gly Met Leu Gly Ala Asp Thr Asp 185 190 195 ttc aac acc atc tac cgc aag cgc aat gtt ggc cac ctc atg ggc aag 739 Phe Asn Thr Ile Tyr Arg Lys Arg Asn Val Gly His Leu Met Gly Lys 200 205 210 cag gaa tcc ttt gcc aat gag ctt cgc cct gga tac aag cac att ctt 787 Gln Glu Ser Phe Ala Asn Glu Leu Arg Pro Gly Tyr Lys His Ile Leu 215 220 225 cac cac ggc tcc tat ggt gcc gcg gag atc cct tgg cgc tac aac ggt 835 His His Gly Ser Tyr Gly Ala Ala Glu Ile Pro Trp Arg Tyr Asn Gly 230 235 240 245 gta gcc att ggt acc gag gat ctg tgg tac atc ggc gca gac aag acc 883 Val Ala Ile Gly Thr Glu Asp Leu Trp Tyr Ile Gly Ala Asp Lys Thr 250 255 260 tac att ttg agc cag cgc taaggagaac ccagtgacag aaattgcaga 931 Tyr Ile Leu Ser Gln Arg 265 <210> 16 <211> 267 <212> PRT <213> Corynebacterium glutamicum <400> 16 Leu Ser Leu Thr Glu Gly Glu Ile Ile Pro Ile Gln Thr Lys Leu Gly 1 5 10 15 His Trp Arg Asp Val Arg Asp Ser Glu Asp Leu Pro Phe Gln Val Ile 20 25 30 Ala Ser Arg Thr Ser Val Thr Ile Met Glu Asn Thr Leu Glu Trp Thr 35 40 45 Asn Ser Arg Leu Glu Arg Gly Glu Glu Phe Thr Glu Leu Asp Ile Tyr 50 55 60 Ala His Tyr Leu Glu Glu Leu Glu Asp Tyr Ala Leu Gln Phe Thr Phe 65 70 75 80 Asp Val Glu Pro Tyr Phe Thr Asn Leu His Ser Ser Asn Arg Met Leu 85 90 95 Phe Pro Gly Pro Pro Val Asp Phe Pro Ile Asn Ala Glu Thr Arg Cys 100 105 110 Ile Gln Leu Asp Ala Gly Val Ala Val Lys Lys Asp Gly Val Val Leu 115 120 125 Gly Thr Ser Asp Met Ala Arg Ser Leu Pro Arg Thr Ala Ala Gly Gln 130 135 140 Glu Ala Tyr Glu Tyr Phe Phe Lys Val Val Arg Glu Gly Ile Ile Gly 145 150 155 160 Gln Leu Arg Pro Gly Val Ile Cys Ala Asp Val His Glu Ala Thr Leu 165 170 175 Asp Tyr Leu Ser Pro Gln Leu Pro Arg Met Ile Asp Ile Gly Met Leu 180 185 190 Gly Ala Asp Thr Asp Phe Asn Thr Ile Tyr Arg Lys Arg Asn Val Gly 195 200 205 His Leu Met Gly Lys Gln Glu Ser Phe Ala Asn Glu Leu Arg Pro Gly 210 215 220 Tyr Lys His Ile Leu His His Gly Ser Tyr Gly Ala Ala Glu Ile Pro 225 230 235 240 Trp Arg Tyr Asn Gly Val Ala Ile Gly Thr Glu Asp Leu Trp Tyr Ile 245 250 255 Gly Ala Asp Lys Thr Tyr Ile Leu Ser Gln Arg 260 265 <210> 17 <211> 1162 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1132) <223> RXA00502 <400> 17 ctaccggcca tccggagctg acgcctacac catgcaacgc ccacgcttga gcgatcgcaa 60 agatcaacag acagacacag aggggacacc cagctaaacc atg atc gtt ttg gga 115 Met Ile Val Leu Gly 1 5 att gaa agc tcc tgc gat gaa aca ggc gta ggc gta gtc aaa ctt gac 163 Ile Glu Ser Ser Cys Asp Glu Thr Gly Val Gly Val Val Lys Leu Asp 10 15 20 ggc gaa gga aac cta gag atc ctc gcc gac tca gtg gcc tcc tcc atg 211 Gly Glu Gly Asn Leu Glu Ile Leu Ala Asp Ser Val Ala Ser Ser Met 25 30 35 caa gaa cat gcc cgc ttt ggt ggc gtc gtg cca gaa atc gcc tcc cgg 259 Gln Glu His Ala Arg Phe Gly Gly Val Val Pro Glu Ile Ala Ser Arg 40 45 50 gcg cac ctg gaa tct atg gtc ccc gtg atg cgt gaa gcg ttg agg cag 307 Ala His Leu Glu Ser Met Val Pro Val Met Arg Glu Ala Leu Arg Gln 55 60 65 gcg ggc gtc gac agg cca gat gct gtg gct gca acc gtg ggc cct ggt 355 Ala Gly Val Asp Arg Pro Asp Ala Val Ala Ala Thr Val Gly Pro Gly 70 75 80 85 ttg gcg ggc gcg ctg ctc gtt gga gcc agc gct gcg aag gcg tat gcc 403 Leu Ala Gly Ala Leu Leu Val Gly Ala Ser Ala Ala Lys Ala Tyr Ala 90 95 100 gct gcg tgg gga gtt ccg ttt tac gcg gtc aac cac ctg ggc gga cac 451 Ala Ala Trp Gly Val Pro Phe Tyr Ala Val Asn His Leu Gly Gly His 105 110 115 gtc gcc gtg gcc aat ctg gaa ggt gaa act ctt cca cac gcg gtg gct 499 Val Ala Val Ala Asn Leu Glu Gly Glu Thr Leu Pro His Ala Val Ala 120 125 130 ttg ctg gtt tcc ggc gga cac act caa ttg ttg gaa gtc gac gcg gtg 547 Leu Leu Val Ser Gly Gly His Thr Gln Leu Leu Glu Val Asp Ala Val 135 140 145 gga tta ccc atg aag gaa ttg gga tcc acc ctc gac gat gcc gct ggc 595 Gly Leu Pro Met Lys Glu Leu Gly Ser Thr Leu Asp Asp Ala Ala Gly 150 155 160 165 gaa gcc tat gac aaa gtc tca agg ctg ttg gga ttg ggc tac cca ggc 643 Glu Ala Tyr Asp Lys Val Ser Arg Leu Leu Gly Leu Gly Tyr Pro Gly 170 175 180 ggc ccc atc att gat aaa ttg gcg cgc cgg ggt aat cca gag gcc att 691 Gly Pro Ile Ile Asp Lys Leu Ala Arg Arg Gly Asn Pro Glu Ala Ile 185 190 195 gct ttc ccc cgc gga ttg atg aaa aag tcg gat tct cgg cat gat ttc 739 Ala Phe Pro Arg Gly Leu Met Lys Lys Ser Asp Ser Arg His Asp Phe 200 205 210 agc ttt tcc ggt ttg aaa acc tcc gtt gcc cgc tac gtg gaa gct gcg 787 Ser Phe Ser Gly Leu Lys Thr Ser Val Ala Arg Tyr Val Glu Ala Ala 215 220 225 gaa aga aac ggt gaa gtt att tcc gtg gag gac gtc tgc gca tca ttc 835 Glu Arg Asn Gly Glu Val Ile Ser Val Glu Asp Val Cys Ala Ser Phe 230 235 240 245 caa gaa gcg gtg tgt gat gtg ttg acg ttt aag gcc gtg cgt gcg tgc 883 Gln Glu Ala Val Cys Asp Val Leu Thr Phe Lys Ala Val Arg Ala Cys 250 255 260 cgc gat gtc ggt gcg aag gtg ctg ctg ttg ggt gga gga gtg gct gcc 931 Arg Asp Val Gly Ala Lys Val Leu Leu Leu Gly Gly Gly Val Ala Ala 265 270 275 aac tct cgt ctg cgg gag ctt gct caa gaa cgt tgc gat aaa gcc gac 979 Asn Ser Arg Leu Arg Glu Leu Ala Gln Glu Arg Cys Asp Lys Ala Asp 280 285 290 atc gaa ctc cgg gtt cct cgt ttc aat ttg tgc acc gat aat ggt gtc 1027 Ile Glu Leu Arg Val Pro Arg Phe Asn Leu Cys Thr Asp Asn Gly Val 295 300 305 atg att gca gcg ttg gcg gct caa aga atc cac gaa ggt gcc caa gaa 1075 Met Ile Ala Ala Leu Ala Ala Gln Arg Ile His Glu Gly Ala Gln Glu 310 315 320 325 tca cca att tcg gtc gga act gat cct tct ttg tcc gtt gag acc cca 1123 Ser Pro Ile Ser Val Gly Thr Asp Pro Ser Leu Ser Val Glu Thr Pro 330 335 340 cag gtg ttt taaacattta gtattagttc catgaaacca 1162 Gln val phe <210> 18 <211> 344 <212> PRT <213> Corynebacterium glutamicum <400> 18 Met Ile Val Leu Gly Ile Glu Ser Ser Cys Asp Glu Thr Gly Val Gly 1 5 10 15 Val Val Lys Leu Asp Gly Glu Gly Asn Leu Glu Ile Leu Ala Asp Ser 20 25 30 Val Ala Ser Ser Met Gln Glu His Ala Arg Phe Gly Gly Val Val Pro 35 40 45 Glu Ile Ala Ser Arg Ala His Leu Glu Ser Met Val Pro Val Met Arg 50 55 60 Glu Ala Leu Arg Gln Ala Gly Val Asp Arg Pro Asp Ala Val Ala Ala 65 70 75 80 Thr Val Gly Pro Gly Leu Ala Gly Ala Leu Leu Val Gly Ala Ser Ala 85 90 95 Ala Lys Ala Tyr Ala Ala Ala Trp Gly Val Pro Phe Tyr Ala Val Asn 100 105 110 His Leu Gly Gly His Val Ala Val Ala Asn Leu Glu Gly Glu Thr Leu 115 120 125 Pro His Ala Val Ala Leu Leu Val Ser Gly Gly His Thr Gln Leu Leu 130 135 140 Glu Val Asp Ala Val Gly Leu Pro Met Lys Glu Leu Gly Ser Thr Leu 145 150 155 160 Asp Asp Ala Ala Gly Glu Ala Tyr Asp Lys Val Ser Arg Leu Leu Gly 165 170 175 Leu Gly Tyr Pro Gly Gly Pro Ile Ile Asp Lys Leu Ala Arg Arg Gly 180 185 190 Asn Pro Glu Ala Ile Ala Phe Pro Arg Gly Leu Met Lys Lys Ser Asp 195 200 205 Ser Arg His Asp Phe Ser Phe Ser Gly Leu Lys Thr Ser Val Ala Arg 210 215 220 Tyr Val Glu Ala Ala Glu Arg Asn Gly Glu Val Ile Ser Val Glu Asp 225 230 235 240 Val Cys Ala Ser Phe Gln Glu Ala Val Cys Asp Val Leu Thr Phe Lys 245 250 255 Ala Val Arg Ala Cys Arg Asp Val Gly Ala Lys Val Leu Leu Leu Gly 260 265 270 Gly Gly Val Ala Ala Asn Ser Arg Leu Arg Glu Leu Ala Gln Glu Arg 275 280 285 Cys Asp Lys Ala Asp Ile Glu Leu Arg Val Pro Arg Phe Asn Leu Cys 290 295 300 Thr Asp Asn Gly Val Met Ile Ala Ala Leu Ala Ala Gln Arg Ile His 305 310 315 320 Glu Gly Ala Gln Glu Ser Pro Ile Ser Val Gly Thr Asp Pro Ser Leu 325 330 335 Ser Val Glu Thr Pro Gln Val Phe 340 <210> 19 <211> 2290 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (2260) <223> RXA00550 <400> 19 gccagctgat tctcagattc agatgaatag gcttaggcgc agtattctct acagcacaag 60 cttcagataa gaaccagatc tgattgaacg gaaacaccag ttg acg aat agt aaa 115 Leu Thr Asn Ser Lys 1 5 aat cct cct gcc aaa aag agc agc ggc aaa agg cgc tta aag agc gac 163 Asn Pro Pro Ala Lys Lys Ser Ser Gly Lys Arg Arg Leu Lys Ser Asp 10 15 20 tct aag ggc tac gct gta aga aac acc atc att ggt gcc att gtt gct 211 Ser Lys Gly Tyr Ala Val Arg Asn Thr Ile Ile Gly Ala Ile Val Ala 25 30 35 gtc att ttg att cca gta atg gtc ttc atg ggt gct tac atc atg gtt 259 Val Ile Leu Ile Pro Val Met Val Phe Met Gly Ala Tyr Ile Met Val 40 45 50 gat gtt cca gaa ccg gaa gag ttg gtt tca ccc cag gtt tcg cag att 307 Asp Val Pro Glu Pro Glu Glu Leu Val Ser Pro Gln Val Ser Gln Ile 55 60 65 tac gca tct gac ggt gag act gaa ttg gca cgc atc gtt cct cca gaa 355 Tyr Ala Ser Asp Gly Glu Thr Glu Leu Ala Arg Ile Val Pro Pro Glu 70 75 80 85 ggc aac cgc cag atg gtg acg atc gat cag gtg cct gac act gtg aaa 403 Gly Asn Arg Gln Met Val Thr Ile Asp Gln Val Pro Asp Thr Val Lys 90 95 100 aat gcg gtg gtg gct gcg gaa gac cga gag ttt tac aca aac ccc ggt 451 Asn Ala Val Val Ala Ala Glu Asp Arg Glu Phe Tyr Thr Asn Pro Gly 105 110 115 ttt tcc att act ggc tat gcc cga gca gca ctt ggc gta atc act ggt 499 Phe Ser Ile Thr Gly Tyr Ala Arg Ala Ala Leu Gly Val Ile Thr Gly 120 125 130 gat tct tca gcg ggt ggt ggt tcc acc att act cag cag tat gtg aag 547 Asp Ser Ser Ala Gly Gly Gly Ser Thr Ile Thr Gln Gln Tyr Val Lys 135 140 145 aag gct gtg gtt ggt gat gag cgt tcg ctg atc cgt aag gct aag gaa 595 Lys Ala Val Val Gly Asp Glu Arg Ser Leu Ile Arg Lys Ala Lys Glu 150 155 160 165 ttg gtc tat tcc gcg aag atg gcc aat gag tgg tct aag gac gag gtc 643 Leu Val Tyr Ser Ala Lys Met Ala Asn Glu Trp Ser Lys Asp Glu Val 170 175 180 ctt gag gct tat ctc aac act gtg tac ttc ggt cga aat gcc tat ggt 691 Leu Glu Ala Tyr Leu Asn Thr Val Tyr Phe Gly Arg Asn Ala Tyr Gly 185 190 195 gtg cag gct gca gct cat gca ttc ttt gat aag cca gta gaa gag ctc 739 Val Gln Ala Ala Ala His Ala Phe Phe Asp Lys Pro Val Glu Glu Leu 200 205 210 acg gct gct gag ggc gca gtg ctg gcg gcc agt att cag ctg cca agc 787 Thr Ala Ala Glu Gly Ala Val Leu Ala Ala Ser Ile Gln Leu Pro Ser 215 220 225 cag ttg gat cct tgg aca aat cca gtt gag gcg gaa acg cgt tgg aac 835 Gln Leu Asp Pro Trp Thr Asn Pro Val Glu Ala Glu Thr Arg Trp Asn 230 235 240 245 tat gtc atg gac ggc ctg gtg gaa att ggc gct atc tcg gca gag gag 883 Tyr Val Met Asp Gly Leu Val Glu Ile Gly Ala Ile Ser Ala Glu Glu 250 255 260 cgc gca gtt gct acc tac cct gaa acc act gac cct gcg tcc aac agt 931 Arg Ala Val Ala Thr Tyr Pro Glu Thr Thr Asp Pro Ala Ser Asn Ser 265 270 275 gcg tac acc gaa gcc acc ggc act aat ggt ttg att aag aac caa gtg 979 Ala Tyr Thr Glu Ala Thr Gly Thr Asn Gly Leu Ile Lys Asn Gln Val 280 285 290 atg gcg gag ttg tct gag ctt ggt atc act gag gat gat gtg caa acc 1027 Met Ala Glu Leu Ser Glu Leu Gly Ile Thr Glu Asp Asp Val Gln Thr 295 300 305 cgt ggt ttg cag gtc acc acc acc att gat cca aag act cag gaa ggt 1075 Arg Gly Leu Gln Val Thr Thr Thr Ile Asp Pro Lys Thr Gln Glu Gly 310 315 320 325 gcc gtt gaa gcg gta caa aac cag ttg gat ctt ctg tct gag aac aac 1123 Ala Val Glu Ala Val Gln Asn Gln Leu Asp Leu Leu Ser Glu Asn Asn 330 335 340 cgt gca gcg gta gtc tcc att gat cct tct aat ggt gcg gtt cgt gct 1171 Arg Ala Ala Val Val Ser Ile Asp Pro Ser Asn Gly Ala Val Arg Ala 345 350 355 tat tac ggc ggc gag gat gcg act ggt tgg gac ttt gca aac gct ccg 1219 Tyr Tyr Gly Gly Glu Asp Ala Thr Gly Trp Asp Phe Ala Asn Ala Pro 360 365 370 ctt cag acc ggt tct aca ttc aag atc ttt ggt ctg gca gca gca ctt 1267 Leu Gln Thr Gly Ser Thr Phe Lys Ile Phe Gly Leu Ala Ala Ala Leu 375 380 385 cag caa ggt att cca ctg tct cag cca tac agc tct gcg ccg gtg act 1315 Gln Gln Gly Ile Pro Leu Ser Gln Pro Tyr Ser Ser Ala Pro Val Thr 390 395 400 405 gtg ggt gat gct caa atc gga aac gtc ggt ggc agc ggt tgt ggt tcc 1363 Val Gly Asp Ala Gln Ile Gly Asn Val Gly Gly Ser Gly Cys Gly Ser 410 415 420 tgt tcc atc gag cag gcg ttg ttg cat tct tac aac acc agc ttc att 1411 Cys Ser Ile Glu Gln Ala Leu Leu His Ser Tyr Asn Thr Ser Phe Ile 425 430 435 cgt ttg cag cag gat ctg gaa aat ggt tca cag gat act gcg gac atg 1459 Arg Leu Gln Gln Asp Leu Glu Asn Gly Ser Gln Asp Thr Ala Asp Met 440 445 450 gcg cat gct ttg ggt atc gcg aag tct ttg cca act atc cct gag aca 1507 Ala His Ala Leu Gly Ile Ala Lys Ser Leu Pro Thr Ile Pro Glu Thr 455 460 465 ctg act gaa aac gga gag acc cct tat gag ggc atc atc ttg ggt cag 1555 Leu Thr Glu Asn Gly Glu Thr Pro Tyr Glu Gly Ile Ile Leu Gly Gln 470 475 480 485 tat gag tcc cgc cca ctt gat atg gct tct gcg atg gca act atc gct 1603 Tyr Glu Ser Arg Pro Leu Asp Met Ala Ser Ala Met Ala Thr Ile Ala 490 495 500 aat gaa ggt gtc tgg cac cgc ccg cac ttc gtg tcc aag gtg gag act 1651 Asn Glu Gly Val Trp His Arg Pro His Phe Val Ser Lys Val Glu Thr 505 510 515 gtc agc ggt gag gtt ctc tac gag ttc gag gat ggc gac ggc gag cgt 1699 Val Ser Gly Glu Val Leu Tyr Glu Phe Glu Asp Gly Asp Gly Glu Arg 520 525 530 cgt gtt tct gaa aag gtt gca ctg aat ctg ctc aag gcc atg ggg cca 1747 Arg Val Ser Glu Lys Val Ala Leu Asn Leu Leu Lys Ala Met Gly Pro 535 540 545 atc gct gca tac tcc aac gga aac gct ctg gct gat ggc cag gtt tct 1795 Ile Ala Ala Tyr Ser Asn Gly Asn Ala Leu Ala Asp Gly Gln Val Ser 550 555 560 565 gca tcc aag act ggt acc act cag ctt ggt gat acc ggt gca aac aag 1843 Ala Ser Lys Thr Gly Thr Thr Gln Leu Gly Asp Thr Gly Ala Asn Lys 570 575 580 gat gcg tgg atg ttg ggt gcg gca cct cag cta gct act gcg gtg tgg 1891 Asp Ala Trp Met Leu Gly Ala Ala Pro Gln Leu Ala Thr Ala Val Trp 585 590 595 gtc gga act gct gat aac act gca ttg tat aac acc tgg ggt ggc agt 1939 Val Gly Thr Ala Asp Asn Thr Ala Leu Tyr Asn Thr Trp Gly Gly Ser 600 605 610 atg tat ggt tct aac tcc cct gcc acg atc tgg aag cag acc atg gat 1987 Met Tyr Gly Ser Asn Ser Pro Ala Thr Ile Trp Lys Gln Thr Met Asp 615 620 625 aac gcc ctc gag aac tcc cct ctc gaa act tgg gat atc gct cca gca 2035 Asn Ala Leu Glu Asn Ser Pro Leu Glu Thr Trp Asp Ile Ala Pro Ala 630 635 640 645 ttg ggg tac ggt aac cca cca gtt ccg gaa tat gtg tgg act cca agt 2083 Leu Gly Tyr Gly Asn Pro Pro Val Pro Glu Tyr Val Trp Thr Pro Ser 650 655 660 cca aac atc gcg act aat gat cca gaa gga gca acc gag gaa gct cca 2131 Pro Asn Ile Ala Thr Asn Asp Pro Glu Gly Ala Thr Glu Glu Ala Pro 665 670 675 gtg gag gat cca aat gca gta atc gat acc cct gct gta gat ccc act 2179 Val Glu Asp Pro Asn Ala Val Ile Asp Thr Pro Ala Val Asp Pro Thr 680 685 690 gca cct gca gag gag acc ggt aac ggt cag gta gaa atc ctg ccg ggg 2227 Ala Pro Ala Glu Glu Thr Gly Asn Gly Gln Val Glu Ile Leu Pro Gly 695 700 705 ctg act atc ccg gga gat ctc tta ggg atc ggc taaaatccgg tcgtagccta 2280 Leu Thr Ile Pro Gly Asp Leu Leu Gly Ile Gly 710 715 720 aacgacctta 2290 <210> 20 <211> 720 <212> PRT <213> Corynebacterium glutamicum <400> 20 Leu Thr Asn Ser Lys Asn Pro Pro Ala Lys Lys Ser Ser Gly Lys Arg 1 5 10 15 Arg Leu Lys Ser Asp Ser Lys Gly Tyr Ala Val Arg Asn Thr Ile Ile 20 25 30 Gly Ala Ile Val Ala Val Ile Leu Ile Pro Val Met Val Phe Met Gly 35 40 45 Ala Tyr Ile Met Val Asp Val Pro Glu Pro Glu Glu Leu Val Ser Pro 50 55 60 Gln Val Ser Gln Ile Tyr Ala Ser Asp Gly Glu Thr Glu Leu Ala Arg 65 70 75 80 Ile Val Pro Pro Glu Gly Asn Arg Gln Met Val Thr Ile Asp Gln Val 85 90 95 Pro Asp Thr Val Lys Asn Ala Val Val Ala Ala Glu Asp Arg Glu Phe 100 105 110 Tyr Thr Asn Pro Gly Phe Ser Ile Thr Gly Tyr Ala Arg Ala Ala Leu 115 120 125 Gly Val Ile Thr Gly Asp Ser Ser Ala Gly Gly Gly Ser Thr Ile Thr 130 135 140 Gln Gln Tyr Val Lys Lys Ala Val Val Gly Asp Glu Arg Ser Leu Ile 145 150 155 160 Arg Lys Ala Lys Glu Leu Val Tyr Ser Ala Lys Met Ala Asn Glu Trp 165 170 175 Ser Lys Asp Glu Val Leu Glu Ala Tyr Leu Asn Thr Val Tyr Phe Gly 180 185 190 Arg Asn Ala Tyr Gly Val Gln Ala Ala Ala His Ala Phe Phe Asp Lys 195 200 205 Pro Val Glu Glu Leu Thr Ala Ala Glu Gly Ala Val Leu Ala Ala Ser 210 215 220 Ile Gln Leu Pro Ser Gln Leu Asp Pro Trp Thr Asn Pro Val Glu Ala 225 230 235 240 Glu Thr Arg Trp Asn Tyr Val Met Asp Gly Leu Val Glu Ile Gly Ala 245 250 255 Ile Ser Ala Glu Glu Arg Ala Val Ala Thr Tyr Pro Glu Thr Thr Asp 260 265 270 Pro Ala Ser Asn Ser Ala Tyr Thr Glu Ala Thr Gly Thr Asn Gly Leu 275 280 285 Ile Lys Asn Gln Val Met Ala Glu Leu Ser Glu Leu Gly Ile Thr Glu 290 295 300 Asp Asp Val Gln Thr Arg Gly Leu Gln Val Thr Thr Thr Ile Asp Pro 305 310 315 320 Lys Thr Gln Glu Gly Ala Val Glu Ala Val Gln Asn Gln Leu Asp Leu 325 330 335 Leu Ser Glu Asn Asn Arg Ala Ala Val Val Ser Ile Asp Pro Ser Asn 340 345 350 Gly Ala Val Arg Ala Tyr Tyr Gly Gly Glu Asp Ala Thr Gly Trp Asp 355 360 365 Phe Ala Asn Ala Pro Leu Gln Thr Gly Ser Thr Phe Lys Ile Phe Gly 370 375 380 Leu Ala Ala Ala Leu Gln Gln Gly Ile Pro Leu Ser Gln Pro Tyr Ser 385 390 395 400 Ser Ala Pro Val Thr Val Gly Asp Ala Gln Ile Gly Asn Val Gly Gly 405 410 415 Ser Gly Cys Gly Ser Cys Ser Ile Glu Gln Ala Leu Leu His Ser Tyr 420 425 430 Asn Thr Ser Phe Ile Arg Leu Gln Gln Asp Leu Glu Asn Gly Ser Gln 435 440 445 Asp Thr Ala Asp Met Ala His Ala Leu Gly Ile Ala Lys Ser Leu Pro 450 455 460 Thr Ile Pro Glu Thr Leu Thr Glu Asn Gly Glu Thr Pro Tyr Glu Gly 465 470 475 480 Ile Ile Leu Gly Gln Tyr Glu Ser Arg Pro Leu Asp Met Ala Ser Ala 485 490 495 Met Ala Thr Ile Ala Asn Glu Gly Val Trp His Arg Pro His Phe Val 500 505 510 Ser Lys Val Glu Thr Val Ser Gly Glu Val Leu Tyr Glu Phe Glu Asp 515 520 525 Gly Asp Gly Glu Arg Arg Val Ser Glu Lys Val Ala Leu Asn Leu Leu 530 535 540 Lys Ala Met Gly Pro Ile Ala Ala Tyr Ser Asn Gly Asn Ala Leu Ala 545 550 555 560 Asp Gly Gln Val Ser Ala Ser Lys Thr Gly Thr Thr Gln Leu Gly Asp 565 570 575 Thr Gly Ala Asn Lys Asp Ala Trp Met Leu Gly Ala Ala Pro Gln Leu 580 585 590 Ala Thr Ala Val Trp Val Gly Thr Ala Asp Asn Thr Ala Leu Tyr Asn 595 600 605 Thr Trp Gly Gly Ser Met Tyr Gly Ser Asn Ser Pro Ala Thr Ile Trp 610 615 620 Lys Gln Thr Met Asp Asn Ala Leu Glu Asn Ser Pro Leu Glu Thr Trp 625 630 635 640 Asp Ile Ala Pro Ala Leu Gly Tyr Gly Asn Pro Pro Val Pro Glu Tyr 645 650 655 Val Trp Thr Pro Ser Pro Asn Ile Ala Thr Asn Asp Pro Glu Gly Ala 660 665 670 Thr Glu Glu Ala Pro Val Glu Asp Pro Asn Ala Val Ile Asp Thr Pro 675 680 685 Ala Val Asp Pro Thr Ala Pro Ala Glu Glu Thr Gly Asn Gly Gln Val 690 695 700 Glu Ile Leu Pro Gly Leu Thr Ile Pro Gly Asp Leu Leu Gly Ile Gly 705 710 715 720 <210> 21 <211> 754 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (724) <223> RXA00566 <400> 21 gaagctaagg attacggact tgttgaccac gtgattacct tggctgaagg cccaatcagc 60 aactaggcgc acggaaaact ttaaaggaga gaataagatt atg agc aac gga ttc 115 Met Ser Asn Gly Phe 1 5 caa atg cca acg tcc cgt tac gtg ctg cct tcc ttc att gag cag tcc 163 Gln Met Pro Thr Ser Arg Tyr Val Leu Pro Ser Phe Ile Glu Gln Ser 10 15 20 gca tac ggc acc aaa gag acc aac cct tac gca aaa ctc ttt gaa gag 211 Ala Tyr Gly Thr Lys Glu Thr Asn Pro Tyr Ala Lys Leu Phe Glu Glu 25 30 35 cgc atc atc ttc ctg ggc acc cag gtc gac gac acc tct gca aac gac 259 Arg Ile Ile Phe Leu Gly Thr Gln Val Asp Asp Thr Ser Ala Asn Asp 40 45 50 atc atg gcg cag ctc ctt gtc ctc gaa ggc atg gac cca gac cgc gat 307 Ile Met Ala Gln Leu Leu Val Leu Glu Gly Met Asp Pro Asp Arg Asp 55 60 65 atc acc ctg tac atc aac tca cct ggt gga tcc ttc acc gcg ttg atg 355 Ile Thr Leu Tyr Ile Asn Ser Pro Gly Gly Ser Phe Thr Ala Leu Met 70 75 80 85 gca att tac gac acc atg cag tac gtc cgc cca gac gtt cag acc gtc 403 Ala Ile Tyr Asp Thr Met Gln Tyr Val Arg Pro Asp Val Gln Thr Val 90 95 100 tgc ctt ggt cag gca gca tcc gca gcc gca gtt ctt ctt gca gcc ggt 451 Cys Leu Gly Gln Ala Ala Ser Ala Ala Ala Val Leu Leu Ala Ala Gly 105 110 115 gca cca ggt aag cgc gct gtt ctt cct aac tcc cgc gtg ctg atc cac 499 Ala Pro Gly Lys Arg Ala Val Leu Pro Asn Ser Arg Val Leu Ile His 120 125 130 cag cca gca acc cag ggc acc cag ggt cag gtt tct gac ctc gag atc 547 Gln Pro Ala Thr Gln Gly Thr Gln Gly Gln Val Ser Asp Leu Glu Ile 135 140 145 cag gca gct gaa atc gag cgc atg cgt cgt ttg atg gaa acc acc ttg 595 Gln Ala Ala Glu Ile Glu Arg Met Arg Arg Leu Met Glu Thr Thr Leu 150 155 160 165 gct gag cac acc ggc aag acc gcg gag cag atc cgc atc gat acc gac 643 Ala Glu His Thr Gly Lys Thr Ala Glu Gln Ile Arg Ile Asp Thr Asp 170 175 180 cgt gac aag atc ctc acc gct gag gaa gca ctc gag tat ggc atc gtt 691 Arg Asp Lys Ile Leu Thr Ala Glu Glu Ala Leu Glu Tyr Gly Ile Val 185 190 195 gac cag gtc ttc gat tac cgc aag ctc aag cgc tagagttttt taaagattcg 744 Asp Gln Val Phe Asp Tyr Arg Lys Leu Lys Arg 200 205 ggtttggttc 754 <210> 22 <211> 208 <212> PRT <213> Corynebacterium glutamicum <400> 22 Met Ser Asn Gly Phe Gln Met Pro Thr Ser Arg Tyr Val Leu Pro Ser 1 5 10 15 Phe Ile Glu Gln Ser Ala Tyr Gly Thr Lys Glu Thr Asn Pro Tyr Ala 20 25 30 Lys Leu Phe Glu Glu Arg Ile Ile Phe Leu Gly Thr Gln Val Asp Asp 35 40 45 Thr Ser Ala Asn Asp Ile Met Ala Gln Leu Leu Val Leu Glu Gly Met 50 55 60 Asp Pro Asp Arg Asp Ile Thr Leu Tyr Ile Asn Ser Pro Gly Gly Ser 65 70 75 80 Phe Thr Ala Leu Met Ala Ile Tyr Asp Thr Met Gln Tyr Val Arg Pro 85 90 95 Asp Val Gln Thr Val Cys Leu Gly Gln Ala Ala Ser Ala Ala Ala Val 100 105 110 Leu Leu Ala Ala Gly Ala Pro Gly Lys Arg Ala Val Leu Pro Asn Ser 115 120 125 Arg Val Leu Ile His Gln Pro Ala Thr Gln Gly Thr Gln Gly Gln Val 130 135 140 Ser Asp Leu Glu Ile Gln Ala Ala Glu Ile Glu Arg Met Arg Arg Leu 145 150 155 160 Met Glu Thr Thr Leu Ala Glu His Thr Gly Lys Thr Ala Glu Gln Ile 165 170 175 Arg Ile Asp Thr Asp Arg Asp Lys Ile Leu Thr Ala Glu Glu Ala Leu 180 185 190 Glu Tyr Gly Ile Val Asp Gln Val Phe Asp Tyr Arg Lys Leu Lys Arg 195 200 205 <210> 23 <211> 1714 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1684) <223> RXA00641 <400> 23 catgcgaaca cctacccccg agcaaaccct aggaattccc ctaatgtgat tcacttcatt 60 gatcgggcac tgttggtcat acgccatcga cactgcggaa ttg ctc gca aat gtc 115 Leu Leu Ala Asn Val 1 5 aca cac cgc ttc aaa gca aaa acg aaa acg aca tcg cgg tgg caa tac 163 Thr His Arg Phe Lys Ala Lys Thr Lys Thr Thr Ser Arg Trp Gln Tyr 10 15 20 caa ctt ctt ttc act ctc ttg gag gtt cac atg tcc aca cca gtt tca 211 Gln Leu Leu Phe Thr Leu Leu Glu Val His Met Ser Thr Pro Val Ser 25 30 35 aat ttg gca agc gtt cag aaa act ctg gac cat gcg ctt gag gac cgc 259 Asn Leu Ala Ser Val Gln Lys Thr Leu Asp His Ala Leu Glu Asp Arg 40 45 50 cct gaa gag gga atc gtc cgc gtc aac cgc aac atc ttc act gac cct 307 Pro Glu Glu Gly Ile Val Arg Val Asn Arg Asn Ile Phe Thr Asp Pro 55 60 65 gag atc ttc gag ctg gag atg cgc cac atc ttc gaa ggc atc tgg atc 355 Glu Ile Phe Glu Leu Glu Met Arg His Ile Phe Glu Gly Ile Trp Ile 70 75 80 85 tac ctg gct cac gag tcc cag atc cct aac ggt ggc gac tac ttc acc 403 Tyr Leu Ala His Glu Ser Gln Ile Pro Asn Gly Gly Asp Tyr Phe Thr 90 95 100 acc tac att ggc cgc cag cgg atc atg atc acc cgt tcc aag gac ggc 451 Thr Tyr Ile Gly Arg Gln Arg Ile Met Ile Thr Arg Ser Lys Asp Gly 105 110 115 aca ctc aac tgc ctg atc aac gcg tgt tct cac cgt ggc gcc atg ctc 499 Thr Leu Asn Cys Leu Ile Asn Ala Cys Ser His Arg Gly Ala Met Leu 120 125 130 tgc cgt cgc aag act gac aac cgc acc acc ttg acc tgc cca ttc cac 547 Cys Arg Arg Lys Thr Asp Asn Arg Thr Thr Leu Thr Cys Pro Phe His 135 140 145 ggc tgg aca ttc agc aac gac ggc gca ctg ctc aag gtc aag gac gaa 595 Gly Trp Thr Phe Ser Asn Asp Gly Ala Leu Leu Lys Val Lys Asp Glu 150 155 160 165 aaa gaa ggc gcc tac cca gag aat ttc cgc acc gac ggc tcc cac gat 643 Lys Glu Gly Ala Tyr Pro Glu Asn Phe Arg Thr Asp Gly Ser His Asp 170 175 180 ctg cgt cgc gtt cct aag ttc gag tcc tac cgt ggc ttc ctc ttc ggc 691 Leu Arg Arg Val Pro Lys Phe Glu Ser Tyr Arg Gly Phe Leu Phe Gly 185 190 195 tcc ctc aac gat gat gtc gtt tct ttg gaa gag cac ctc ggc gac acc 739 Ser Leu Asn Asp Asp Val Val Ser Leu Glu Glu His Leu Gly Asp Thr 200 205 210 cgt acc gtc att gac atg ctg gtt gac caa tcc cca gaa ggc ctc gaa 787 Arg Thr Val Ile Asp Met Leu Val Asp Gln Ser Pro Glu Gly Leu Glu 215 220 225 gta ctg cgc gga tcc tcc acc tac acc tac gac ggc aac tgg aag ctc 835 Val Leu Arg Gly Ser Ser Thr Tyr Thr Tyr Asp Gly Asn Trp Lys Leu 230 235 240 245 cag acc gaa aac ggt gca gac ggc tac cac gtt tcc tcc acc cac tgg 883 Gln Thr Glu Asn Gly Ala Asp Gly Tyr His Val Ser Ser Thr His Trp 250 255 260 aac tac gct gca acc acc tcc cgc cgt ggc act ggt gaa tcc gcg aac 931 Asn Tyr Ala Ala Thr Thr Ser Arg Arg Gly Thr Gly Glu Ser Ala Asn 265 270 275 gaa acc aag gca atg gac gct ggt acc tgg ggc aag cag ggt ggc gga 979 Glu Thr Lys Ala Met Asp Ala Gly Thr Trp Gly Lys Gln Gly Gly Gly 280 285 290 tac ttc tcc tac cct tac ggc cac atg ctg ctg tgg atg tgg tgg ggc 1027 Tyr Phe Ser Tyr Pro Tyr Gly His Met Leu Leu Trp Met Trp Trp Gly 295 300 305 aac cca gaa gac cgc cca ctg ttc gag cgc cgc gac gag ttc aag aag 1075 Asn Pro Glu Asp Arg Pro Leu Phe Glu Arg Arg Asp Glu Phe Lys Lys 310 315 320 325 gaa ttc ggc gaa gaa aag ggc gag ttc atg gtt ggt gct tcc cgc aat 1123 Glu Phe Gly Glu Glu Lys Gly Glu Phe Met Val Gly Ala Ser Arg Asn 330 335 340 ctg tgc ctc tac ccc aat gtt tac ctg atg gat cag ttc tcc tca cag 1171 Leu Cys Leu Tyr Pro Asn Val Tyr Leu Met Asp Gln Phe Ser Ser Gln 345 350 355 atc cgc cac atc cgc cca atc tca gtt gat cag acc gaa gtc acc atc 1219 Ile Arg His Ile Arg Pro Ile Ser Val Asp Gln Thr Glu Val Thr Ile 360 365 370 tac tgc atc gca cct aag ggc gag tcc gcg gaa gca cgt gca aac cgc 1267 Tyr Cys Ile Ala Pro Lys Gly Glu Ser Ala Glu Ala Arg Ala Asn Arg 375 380 385 atc cgc cag tac gaa gac ttc ttc aac gca acg ggc atg gca acc cca 1315 Ile Arg Gln Tyr Glu Asp Phe Phe Asn Ala Thr Gly Met Ala Thr Pro 390 395 400 405 gat gac ctg gag gaa ttc cgc tcc tgc cag aag acc tac cag gca tct 1363 Asp Asp Leu Glu Glu Phe Arg Ser Cys Gln Lys Thr Tyr Gln Ala Ser 410 415 420 gcc ttc cca tgg aat gac atg acc cgc ggt ttg ggc cac cag gta cag 1411 Ala Phe Pro Trp Asn Asp Met Thr Arg Gly Leu Gly His Gln Val Gln 425 430 435 gga cca aac gag gtt gcc aag ggc cta ggc atg aac gaa gtt ctt tcc 1459 Gly Pro Asn Glu Val Ala Lys Gly Leu Gly Met Asn Glu Val Leu Ser 440 445 450 tcc gga gca cgc acc gaa gat gaa ggc ctc tac cca atc cag cac ggc 1507 Ser Gly Ala Arg Thr Glu Asp Glu Gly Leu Tyr Pro Ile Gln His Gly 455 460 465 ttc tgg cat gaa ctc atg cag gag gct gtg aat aag cag agc atc aag 1555 Phe Trp His Glu Leu Met Gln Glu Ala Val Asn Lys Gln Ser Ile Lys 470 475 480 485 gaa aag gaa ttg gct gac gat acc gct tct tcc ctt gcc acc gta gct 1603 Glu Lys Glu Leu Ala Asp Asp Thr Ala Ser Ser Leu Ala Thr Val Ala 490 495 500 gca gcc aaa atc cgt gag gaa gca aag gca gcc gcg aag tcc gac gct 1651 Ala Ala Lys Ile Arg Glu Glu Ala Lys Ala Ala Ala Lys Ser Asp Ala 505 510 515 gga gag cct cgc cgc cgt cgt cgc acc cgc ggt tagtcgtcga aaagcaaaaa 1704 Gly Glu Pro Arg Arg Arg Arg Arg Thr Arg Gly 520 525 atcttttaag 1714 <210> 24 <211> 528 <212> PRT <213> Corynebacterium glutamicum <400> 24 Leu Leu Ala Asn Val Thr His Arg Phe Lys Ala Lys Thr Lys Thr Thr 1 5 10 15 Ser Arg Trp Gln Tyr Gln Leu Leu Phe Thr Leu Leu Glu Val His Met 20 25 30 Ser Thr Pro Val Ser Asn Leu Ala Ser Val Gln Lys Thr Leu Asp His 35 40 45 Ala Leu Glu Asp Arg Pro Glu Glu Gly Ile Val Arg Val Asn Arg Asn 50 55 60 Ile Phe Thr Asp Pro Glu Ile Phe Glu Leu Glu Met Arg His Ile Phe 65 70 75 80 Glu Gly Ile Trp Ile Tyr Leu Ala His Glu Ser Gln Ile Pro Asn Gly 85 90 95 Gly Asp Tyr Phe Thr Thr Tyr Ile Gly Arg Gln Arg Ile Met Ile Thr 100 105 110 Arg Ser Lys Asp Gly Thr Leu Asn Cys Leu Ile Asn Ala Cys Ser His 115 120 125 Arg Gly Ala Met Leu Cys Arg Arg Lys Thr Asp Asn Arg Thr Thr Leu 130 135 140 Thr Cys Pro Phe His Gly Trp Thr Phe Ser Asn Asp Gly Ala Leu Leu 145 150 155 160 Lys Val Lys Asp Glu Lys Glu Gly Ala Tyr Pro Glu Asn Phe Arg Thr 165 170 175 Asp Gly Ser His Asp Leu Arg Arg Val Pro Lys Phe Glu Ser Tyr Arg 180 185 190 Gly Phe Leu Phe Gly Ser Leu Asn Asp Asp Val Val Ser Leu Glu Glu 195 200 205 His Leu Gly Asp Thr Arg Thr Val Ile Asp Met Leu Val Asp Gln Ser 210 215 220 Pro Glu Gly Leu Glu Val Leu Arg Gly Ser Ser Thr Tyr Thr Tyr Asp 225 230 235 240 Gly Asn Trp Lys Leu Gln Thr Glu Asn Gly Ala Asp Gly Tyr His Val 245 250 255 Ser Ser Thr His Trp Asn Tyr Ala Ala Thr Thr Ser Arg Arg Gly Thr 260 265 270 Gly Glu Ser Ala Asn Glu Thr Lys Ala Met Asp Ala Gly Thr Trp Gly 275 280 285 Lys Gln Gly Gly Gly Tyr Phe Ser Tyr Pro Tyr Gly His Met Leu Leu 290 295 300 Trp Met Trp Trp Gly Asn Pro Glu Asp Arg Pro Leu Phe Glu Arg Arg 305 310 315 320 Asp Glu Phe Lys Lys Glu Phe Gly Glu Glu Lys Gly Glu Phe Met Val 325 330 335 Gly Ala Ser Arg Asn Leu Cys Leu Tyr Pro Asn Val Tyr Leu Met Asp 340 345 350 Gln Phe Ser Ser Gln Ile Arg His Ile Arg Pro Ile Ser Val Asp Gln 355 360 365 Thr Glu Val Thr Ile Tyr Cys Ile Ala Pro Lys Gly Glu Ser Ala Glu 370 375 380 Ala Arg Ala Asn Arg Ile Arg Gln Tyr Glu Asp Phe Phe Asn Ala Thr 385 390 395 400 Gly Met Ala Thr Pro Asp Asp Leu Glu Glu Phe Arg Ser Cys Gln Lys 405 410 415 Thr Tyr Gln Ala Ser Ala Phe Pro Trp Asn Asp Met Thr Arg Gly Leu 420 425 430 Gly His Gln Val Gln Gly Pro Asn Glu Val Ala Lys Gly Leu Gly Met 435 440 445 Asn Glu Val Leu Ser Ser Gly Ala Arg Thr Glu Asp Glu Gly Leu Tyr 450 455 460 Pro Ile Gln His Gly Phe Trp His Glu Leu Met Gln Glu Ala Val Asn 465 470 475 480 Lys Gln Ser Ile Lys Glu Lys Glu Leu Ala Asp Asp Thr Ala Ser Ser 485 490 495 Leu Ala Thr Val Ala Ala Ala Lys Ile Arg Glu Glu Ala Lys Ala Ala 500 505 510 Ala Lys Ser Asp Ala Gly Glu Pro Arg Arg Arg Arg Arg Thr Arg Gly 515 520 525 <210> 25 <211> 1504 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (101) .. (1474) <223> RXA00663 <400> 25 ctgaacgatt ggtgaccggc tcatgaaaac ttgacgagtc cccggtattc gccagcggtg 60 actactaccg tgggcgacaa gcccacttag aggaggactt gtg aca acc acc tat 115 Val Thr Thr Thr Tyr 1 5 cca gat ttc ctt gga aat tct tcg ctc caa aca gat acg gag cac tgg 163 Pro Asp Phe Leu Gly Asn Ser Ser Leu Gln Thr Asp Thr Glu His Trp 10 15 20 gaa atg gaa gga ggt gcg cag gaa gtc tct gtt act tat gtt ttg gac 211 Glu Met Glu Gly Gly Ala Gln Glu Val Ser Val Thr Tyr Val Leu Asp 25 30 35 acg tca gtg ttg ctg tct gat ccg ttg tcg ttg aca cgg ttc gcg gag 259 Thr Ser Val Leu Leu Ser Asp Pro Leu Ser Leu Thr Arg Phe Ala Glu 40 45 50 cac gat gta gtt ctg cca att gtt gta att acg gaa tta gaa gcc aag 307 His Asp Val Val Leu Pro Ile Val Val Ile Thr Glu Leu Glu Ala Lys 55 60 65 cgt cat cac ccg gac ctt ggc ttt ttt gct cgc caa gcg ctt cgg atg 355 Arg His His Pro Asp Leu Gly Phe Phe Ala Arg Gln Ala Leu Arg Met 70 75 80 85 ctg gat gag ctg cgt gag atc cat ggg gat ttg tcc aag cca ctg cca 403 Leu Asp Glu Leu Arg Glu Ile His Gly Asp Leu Ser Lys Pro Leu Pro 90 95 100 att ggc gat gaa ggc gga cac atc cat gtt gag ctg aat cac caa aac 451 Ile Gly Asp Glu Gly Gly His Ile His Val Glu Leu Asn His Gln Asn 105 110 115 acg ggg tcc ttg ccc gtg gga ttc cgc ctt ggt gac aat gac acc cgc 499 Thr Gly Ser Leu Pro Val Gly Phe Arg Leu Gly Asp Asn Asp Thr Arg 120 125 130 atc ctt gca gtg gcc aag aat ctg cag gaa gag ggc cac aat gtg gtt 547 Ile Leu Ala Val Ala Lys Asn Leu Gln Glu Glu Gly His Asn Val Val 135 140 145 ctg gtg tcg aag gac ctg ccg atg cgg att aag gcg tcg gca agc gga 595 Leu Val Ser Lys Asp Leu Pro Met Arg Ile Lys Ala Ser Ala Ser Gly 150 155 160 165 atc gcc gca cag gaa tac cgc gct gcc ctg gcg cgc gac cgt ggt tac 643 Ile Ala Ala Gln Glu Tyr Arg Ala Ala Leu Ala Arg Asp Arg Gly Tyr 170 175 180 acc ggc atg acc cac gcc aat atc acc gat gac cag ctc agc gag ctc 691 Thr Gly Met Thr His Ala Asn Ile Thr Asp Asp Gln Leu Ser Glu Leu 185 190 195 tac gac acc ggc gag gtg cgc att gag gag ctc gaa aag ctg ccc gtc 739 Tyr Asp Thr Gly Glu Val Arg Ile Glu Glu Leu Glu Lys Leu Pro Val 200 205 210 aac cac ggc ttc acc ctc aaa tcc aac agc ggt tcg gcg ctt ggt cgt 787 Asn His Gly Phe Thr Leu Lys Ser Asn Ser Gly Ser Ala Leu Gly Arg 215 220 225 atg aat tcc gac aag atc atc gag ctt gtc ccc ggc gac cag cag gta 835 Met Asn Ser Asp Lys Ile Ile Glu Leu Val Pro Gly Asp Gln Gln Val 230 235 240 245 ttc ggt atc agc ggg cgt agc gct gag cag cgg gtt gcc att gat ttg 883 Phe Gly Ile Ser Gly Arg Ser Ala Glu Gln Arg Val Ala Ile Asp Leu 250 255 260 ctt aac gac gac gcc gtc ggc atc gta tcc atc ggc ggc ccc gcg ggt 931 Leu Asn Asp Asp Ala Val Gly Ile Val Ser Ile Gly Gly Pro Ala Gly 265 270 275 aca ggt aaa agc gca ctc gca ctg tgt gcc ggc ctg gaa gct gtg atg 979 Thr Gly Lys Ser Ala Leu Ala Leu Cys Ala Gly Leu Glu Ala Val Met 280 285 290 gag cgt cgc att cag cgc aag att atc gtg ttc cgc cca ctc ttt gcc 1027 Glu Arg Arg Ile Gln Arg Lys Ile Ile Val Phe Arg Pro Leu Phe Ala 295 300 305 gtt ggc gga cag gaa ctt ggc tac ctg cct ggc gac caa gaa gaa aaa 1075 Val Gly Gly Gln Glu Leu Gly Tyr Leu Pro Gly Asp Gln Glu Glu Lys 310 315 320 325 atg ggg cct tgg gcg caa gcg gtt ttt gac acc cta agc tcc atg gtc 1123 Met Gly Pro Trp Ala Gln Ala Val Phe Asp Thr Leu Ser Ser Met Val 330 335 340 agc caa aac atc atc gat gaa gcc ctc tcc cgc ggc ctc atc gaa gtt 1171 Ser Gln Asn Ile Ile Asp Glu Ala Leu Ser Arg Gly Leu Ile Glu Val 345 350 355 ctc cca ctt act cac atc cgc gga cgc tca ctc cac gat gct ttc gtc 1219 Leu Pro Leu Thr His Ile Arg Gly Arg Ser Leu His Asp Ala Phe Val 360 365 370 atc gtc gac gag gcc caa tcc cta gaa cgc aac gtg ttg ctc acc atg 1267 Ile Val Asp Glu Ala Gln Ser Leu Glu Arg Asn Val Leu Leu Thr Met 375 380 385 ctg tct cgc atc ggc cag aat tcc cga gta gtt ctc acc cat gac gta 1315 Leu Ser Arg Ile Gly Gln Asn Ser Arg Val Val Leu Thr His Asp Val 390 395 400 405 gcg cag cgc gac aac ctg cgc gtt ggt cgc tac gac ggc atc gtc tct 1363 Ala Gln Arg Asp Asn Leu Arg Val Gly Arg Tyr Asp Gly Ile Val Ser 410 415 420 gtg gtg gaa gca ctc aag gat cac gaa ctg ttt ggc cac atc acg ttg 1411 Val Val Glu Ala Leu Lys Asp His Glu Leu Phe Gly His Ile Thr Leu 425 430 435 cag cgt tcc gaa cgc tcc cga atc gct gag ttg gtc acc caa gtt ttg 1459 Gln Arg Ser Glu Arg Ser Arg Ile Ala Glu Leu Val Thr Gln Val Leu 440 445 450 gat gcg ccg tct ctg tagtcgcgca gtctgtggcg atttaagggc 1504 Asp Ala Pro Ser Leu 455 <210> 26 <211> 458 <212> PRT <213> Corynebacterium glutamicum <400> 26 Val Thr Thr Thr Tyr Pro Asp Phe Leu Gly Asn Ser Ser Leu Gln Thr 1 5 10 15 Asp Thr Glu His Trp Glu Met Glu Gly Gly Ala Gln Glu Val Ser Val 20 25 30 Thr Tyr Val Leu Asp Thr Ser Val Leu Leu Ser Asp Pro Leu Ser Leu 35 40 45 Thr Arg Phe Ala Glu His Asp Val Val Leu Pro Ile Val Val Ile Thr 50 55 60 Glu Leu Glu Ala Lys Arg His His Pro Asp Leu Gly Phe Phe Ala Arg 65 70 75 80 Gln Ala Leu Arg Met Leu Asp Glu Leu Arg Glu Ile His Gly Asp Leu 85 90 95 Ser Lys Pro Leu Pro Ile Gly Asp Glu Gly Gly His Ile His Val Glu 100 105 110 Leu Asn His Gln Asn Thr Gly Ser Leu Pro Val Gly Phe Arg Leu Gly 115 120 125 Asp Asn Asp Thr Arg Ile Leu Ala Val Ala Lys Asn Leu Gln Glu Glu 130 135 140 Gly His Asn Val Val Leu Val Ser Lys Asp Leu Pro Met Arg Ile Lys 145 150 155 160 Ala Ser Ala Ser Gly Ile Ala Ala Gln Glu Tyr Arg Ala Ala Leu Ala 165 170 175 Arg Asp Arg Gly Tyr Thr Gly Met Thr His Ala Asn Ile Thr Asp Asp 180 185 190 Gln Leu Ser Glu Leu Tyr Asp Thr Gly Glu Val Arg Ile Glu Glu Leu 195 200 205 Glu Lys Leu Pro Val Asn His Gly Phe Thr Leu Lys Ser Asn Ser Gly 210 215 220 Ser Ala Leu Gly Arg Met Asn Ser Asp Lys Ile Ile Glu Leu Val Pro 225 230 235 240 Gly Asp Gln Gln Val Phe Gly Ile Ser Gly Arg Ser Ala Glu Gln Arg 245 250 255 Val Ala Ile Asp Leu Leu Asn Asp Asp Ala Val Gly Ile Val Ser Ile 260 265 270 Gly Gly Pro Ala Gly Thr Gly Lys Ser Ala Leu Ala Leu Cys Ala Gly 275 280 285 Leu Glu Ala Val Met Glu Arg Arg Ile Gln Arg Lys Ile Ile Val Phe 290 295 300 Arg Pro Leu Phe Ala Val Gly Gly Gln Glu Leu Gly Tyr Leu Pro Gly 305 310 315 320 Asp Gln Glu Glu Lys Met Gly Pro Trp Ala Gln Ala Val Phe Asp Thr 325 330 335 Leu Ser Ser Met Val Ser Gln Asn Ile Ile Asp Glu Ala Leu Ser Arg 340 345 350 Gly Leu Ile Glu Val Leu Pro Leu Thr His Ile Arg Gly Arg Ser Leu 355 360 365 His Asp Ala Phe Val Ile Val Asp Glu Ala Gln Ser Leu Glu Arg Asn 370 375 380 Val Leu Leu Thr Met Leu Ser Arg Ile Gly Gln Asn Ser Arg Val Val 385 390 395 400 Leu Thr His Asp Val Ala Gln Arg Asp Asn Leu Arg Val Gly Arg Tyr 405 410 415 Asp Gly Ile Val Ser Val Val Glu Ala Leu Lys Asp His Glu Leu Phe 420 425 430 Gly His Ile Thr Leu Gln Arg Ser Glu Arg Ser Arg Ile Ala Glu Leu 435 440 445 Val Thr Gln Val Leu Asp Ala Pro Ser Leu 450 455 <210> 27 <211> 370 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. <223> RXA00675 <400> 27 gtggaagtac tcacctcaag gtggccggaa ccgcatgcat tgtgcggtgc cggccacctt 60 tggttttaac agtttaattt gaagaaagag acgtggaagc atg ggt ttc cgt tcc 115 Met Gly Phe Arg Ser 1 5 aag aag aag gtt att gcg gca aag acc gcc gct gag ctg gac gcg atg 163 Lys Lys Lys Val Ile Ala Ala Lys Thr Ala Ala Glu Leu Asp Ala Met 10 15 20 cag gcg gcg ggt gag atc gtc ggc aag gct ttg cag gct gtg cgc gct 211 Gln Ala Ala Gly Glu Ile Val Gly Lys Ala Leu Gln Ala Val Arg Ala 25 30 35 gag gct aaa gct ggc atg agc acg tgg gat ctg gat cag atc gcg gag 259 Glu Ala Lys Ala Gly Met Ser Thr Trp Asp Leu Asp Gln Ile Ala Glu 40 45 50 cag gtt atc cgc gat gct ggc gcc gtt cct aca ttc ctg ggt tac cag 307 Gln Val Ile Arg Asp Ala Gly Ala Val Pro Thr Phe Leu Gly Tyr Gln 55 60 65 ggt ttt ccc ggc atc agt gtg cgc ttc ggt caa tgaggtgatt gttcacggca 360 Gly Phe Pro Gly Ile Ser Val Arg Phe Gly Gln 70 75 80 ttccatccaa 370 <210> 28 <211> 80 <212> PRT <213> Corynebacterium glutamicum <400> 28 Met Gly Phe Arg Ser Lys Lys Lys Val Ile Ala Ala Lys Thr Ala Ala 1 5 10 15 Glu Leu Asp Ala Met Gln Ala Ala Gly Glu Ile Val Gly Lys Ala Leu 20 25 30 Gln Ala Val Arg Ala Glu Ala Lys Ala Gly Met Ser Thr Trp Asp Leu 35 40 45 Asp Gln Ile Ala Glu Gln Val Ile Arg Asp Ala Gly Ala Val Pro Thr 50 55 60 Phe Leu Gly Tyr Gln Gly Phe Pro Gly Ile Ser Val Arg Phe Gly Gln 65 70 75 80 <210> 29 <211> 1762 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1732) <223> RXA00689 <400> 29 acgacacccg ccaagtggcc tactcactag aagacgctct tgttcgcgat tgcgaccgac 60 tctggcgttt cgaccgcgcc gggatcacgg cttctagctc atg gtt cgg gat gcc 115 Met val arg asp ala 1 5 agt ctc aga tta tgt cga ccg cct gca cag gga aat cct ccc aga att 163 Ser Leu Arg Leu Cys Arg Pro Pro Ala Gln Gly Asn Pro Pro Arg Ile 10 15 20 aat cac cga agc tgc aca cca gat ggc tac tgc aga cct caa tcg tgc 211 Asn His Arg Ser Cys Thr Pro Asp Gly Tyr Cys Arg Pro Gln Ser Cys 25 30 35 aaa ggc cct gtt aag aac gga tgc gat ccg atg aat gct gca acc agg 259 Lys Gly Pro Val Lys Asn Gly Cys Asp Pro Met Asn Ala Ala Thr Arg 40 45 50 cgt gct tct ctg caa ctc ccc tat acc cat gtc gat gat ttc tac atc 307 Arg Ala Ser Leu Gln Leu Pro Tyr Thr His Val Asp Asp Phe Tyr Ile 55 60 65 aac ggt tcc tgg gtt aaa gca gaa gga aca caa cgc aac ccc gta gtt 355 Asn Gly Ser Trp Val Lys Ala Glu Gly Thr Gln Arg Asn Pro Val Val 70 75 80 85 gat cct gcg gtc ggt caa gaa tgg gga tct gtt cca gaa gca acc gca 403 Asp Pro Ala Val Gly Gln Glu Trp Gly Ser Val Pro Glu Ala Thr Ala 90 95 100 tct gaa ttg gac tct gcg gtg gga gct gca cgt aca gcg cta aag tcg 451 Ser Glu Leu Asp Ser Ala Val Gly Ala Ala Arg Thr Ala Leu Lys Ser 105 110 115 tgg agt gca ctt aca ggt gcg gaa cga aca ggc tac ctc ctg aaa atc 499 Trp Ser Ala Leu Thr Gly Ala Glu Arg Thr Gly Tyr Leu Leu Lys Ile 120 125 130 gcg acg gaa att gaa tcc cgt tct gaa gct cta gca ctt act aat acc 547 Ala Thr Glu Ile Glu Ser Arg Ser Glu Ala Leu Ala Leu Thr Asn Thr 135 140 145 cgc gaa aat ggt tcc ccc att tcc gag acc cgt gga gct gcg tcc aat 595 Arg Glu Asn Gly Ser Pro Ile Ser Glu Thr Arg Gly Ala Ala Ser Asn 150 155 160 165 gca gca gga att ttc cgt tac ttt gcc act ctc gcg cct tgg tta gac 643 Ala Ala Gly Ile Phe Arg Tyr Phe Ala Thr Leu Ala Pro Trp Leu Asp 170 175 180 ggc gaa gac atc cgc cca ttt cct gcc ggt agc gcc gaa tcc atc gtg 691 Gly Glu Asp Ile Arg Pro Phe Pro Ala Gly Ser Ala Glu Ser Ile Val 185 190 195 gat aaa gat ccc atc ggt gtc tgc gca ctc atc gcc cca tgg aat ttc 739 Asp Lys Asp Pro Ile Gly Val Cys Ala Leu Ile Ala Pro Trp Asn Phe 200 205 210 ccg atc aac ctt gta gtc atc aaa ctg gca cca gca ctt ctt gcc ggc 787 Pro Ile Asn Leu Val Val Ile Lys Leu Ala Pro Ala Leu Leu Ala Gly 215 220 225 tgt acc gtc atc atc aaa cca gcc tcc ccc acc cca ctg tcg atc cgt 835 Cys Thr Val Ile Ile Lys Pro Ala Ser Pro Thr Pro Leu Ser Ile Arg 230 235 240 245 ttc atc atc gaa gcc atc gaa gcc gcc gga gtg cca gca ggc gta gtc 883 Phe Ile Ile Glu Ala Ile Glu Ala Ala Gly Val Pro Ala Gly Val Val 250 255 260 aac cta ctc acc ggt tca ggg cgt ttc ggt gat gcc ctt gtc cgc cac 931 Asn Leu Leu Thr Gly Ser Gly Arg Phe Gly Asp Ala Leu Val Arg His 265 270 275 ccc gga gta gac aag gta gcg ttt acc gga tca acg cct gtt gga aag 979 Pro Gly Val Asp Lys Val Ala Phe Thr Gly Ser Thr Pro Val Gly Lys 280 285 290 aag atc gct gcc gcc tgc gga gaa cta ctc cga cca gtg act tta gag 1027 Lys Ile Ala Ala Ala Cys Gly Glu Leu Leu Arg Pro Val Thr Leu Glu 295 300 305 cta ggc gga aaa tct tcc gcg att atc ctt cct gat gca gac atg tca 1075 Leu Gly Gly Lys Ser Ser Ala Ile Lele Pro Asp Ala Asp Met Ser 310 315 320 325 gta ctc tcg acg cgg ttg att cga tcc tgt atg cgc aac act gga caa 1123 Val Leu Ser Thr Arg Leu Ile Arg Ser Cys Met Arg Asn Thr Gly Gln 330 335 340 acc tgc tac atc agt acc cgg att att gcc cct agc tca cgc tat gcg 1171 Thr Cys Tyr Ile Ser Thr Arg Ile Ila Pro Ser Ser Arg Tyr Ala 345 350 355 gaa gtc gta caa aca gtg gca agc act atc gct gca ggt aga caa ggt 1219 Glu Val Val Gln Thr Val Ala Ser Thr Ile Ala Ala Gly Arg Gln Gly 360 365 370 gac ccc tat gat gaa gaa acg gtt ttt ggg cca gtt gcc agc gcc tct 1267 Asp Pro Tyr Asp Glu Glu Thr Val Phe Gly Pro Val Ala Ser Ala Ser 375 380 385 cag tac tca acc gtc atg tct tac att gac tcc gca cga gag gaa ggt 1315 Gln Tyr Ser Thr Val Met Ser Tyr Ile Asp Ser Ala Arg Glu Glu Gly 390 395 400 405 gca cga gtg gtt gca ggt gga acc cgg tca atc agc ctt tct gaa ggt 1363 Ala Arg Val Val Ala Gly Gly Thr Arg Ser Ile Ser Leu Ser Glu Gly 410 415 420 tta gaa tca ggc gag ttt atc caa cca acc gtg ttt gcc gat gtc acc 1411 Leu Glu Ser Gly Glu Phe Ile Gln Pro Thr Val Phe Ala Asp Val Thr 425 430 435 ccc gac atg cgg ata tca cgc gaa gaa atc ttc ggc cct gtt att tcc 1459 Pro Asp Met Arg Ile Ser Arg Glu Glu Ile Phe Gly Pro Val Ile Ser 440 445 450 atc cta aag tac gac gat aca aac ggt gtt tcc gaa gca atc gca cta 1507 Ile Leu Lys Tyr Asp Asp Thr Asn Gly Val Ser Glu Ala Ile Ala Leu 455 460 465 gcc aac aac acg aaa ttc ggt ctc ggt ggc ttg gta ttt ggt gcg gat 1555 Ala Asn Asn Thr Lys Phe Gly Leu Gly Gly Leu Val Phe Gly Ala Asp 470 475 480 485 gag gaa caa gca cta gaa gtc gcc cgt caa gtg gat tct ggt tcc gta 1603 Glu Glu Gln Ala Leu Glu Val Ala Arg Gln Val Asp Ser Gly Ser Val 490 495 500 ggc atc aac ttc ttc ggt tcc aac cat tcc gcc cca ttt gga gga cgc 1651 Gly Ile Asn Phe Phe Gly Ser Asn His Ser Ala Pro Phe Gly Gly Arg 505 510 515 cac gaa tcc ggt atg gga gtg gaa tac ggc atc gaa ggc ctc agt gct 1699 His Glu Ser Gly Met Gly Val Glu Tyr Gly Ile Glu Gly Leu Ser Ala 520 525 530 tac ctg aca tac aag agt att cac cga acc att tagttactga aagttctcag 1752 Tyr Leu Thr Tyr Lys Ser Ile His Arg Thr Ile 535 540 ctaaacaccc 1762 <210> 30 <211> 544 <212> PRT <213> Corynebacterium glutamicum <400> 30 Met Val Arg Asp Ala Ser Leu Arg Leu Cys Arg Pro Pro Ala Gln Gly 1 5 10 15 Asn Pro Pro Arg Ile Asn His Arg Ser Cys Thr Pro Asp Gly Tyr Cys 20 25 30 Arg Pro Gln Ser Cys Lys Gly Pro Val Lys Asn Gly Cys Asp Pro Met 35 40 45 Asn Ala Ala Thr Arg Arg Ala Ser Leu Gln Leu Pro Tyr Thr His Val 50 55 60 Asp Asp Phe Tyr Ile Asn Gly Ser Trp Val Lys Ala Glu Gly Thr Gln 65 70 75 80 Arg Asn Pro Val Val Asp Pro Ala Val Gly Gln Glu Trp Gly Ser Val 85 90 95 Pro Glu Ala Thr Ala Ser Glu Leu Asp Ser Ala Val Gly Ala Ala Arg 100 105 110 Thr Ala Leu Lys Ser Trp Ser Ala Leu Thr Gly Ala Glu Arg Thr Gly 115 120 125 Tyr Leu Leu Lys Ile Ala Thr Glu Ile Glu Ser Arg Ser Glu Ala Leu 130 135 140 Ala Leu Thr Asn Thr Arg Glu Asn Gly Ser Pro Ile Ser Glu Thr Arg 145 150 155 160 Gly Ala Ala Ser Asn Ala Ala Gly Ile Phe Arg Tyr Phe Ala Thr Leu 165 170 175 Ala Pro Trp Leu Asp Gly Glu Asp Ile Arg Pro Phe Pro Ala Gly Ser 180 185 190 Ala Glu Ser Ile Val Asp Lys Asp Pro Ile Gly Val Cys Ala Leu Ile 195 200 205 Ala Pro Trp Asn Phe Pro Ile Asn Leu Val Val Ile Lys Leu Ala Pro 210 215 220 Ala Leu Leu Ala Gly Cys Thr Val Ile Ile Lys Pro Ala Ser Pro Thr 225 230 235 240 Pro Leu Ser Ile Arg Phe Ile Ile Glu Ala Ile Glu Ala Ala Gly Val 245 250 255 Pro Ala Gly Val Val Asn Leu Leu Thr Gly Ser Gly Arg Phe Gly Asp 260 265 270 Ala Leu Val Arg His Pro Gly Val Asp Lys Val Ala Phe Thr Gly Ser 275 280 285 Thr Pro Val Gly Lys Lys Ile Ala Ala Ala Cys Gly Glu Leu Leu Arg 290 295 300 Pro Val Thr Leu Glu Leu Gly Gly Lys Ser Ser Ala Ile Ile Leu Pro 305 310 315 320 Asp Ala Asp Met Ser Val Leu Ser Thr Arg Leu Ile Arg Ser Cys Met 325 330 335 Arg Asn Thr Gly Gln Thr Cys Tyr Ile Ser Thr Arg Ile Ile Ala Pro 340 345 350 Ser Ser Arg Tyr Ala Glu Val Val Gln Thr Val Ala Ser Thr Ile Ala 355 360 365 Ala Gly Arg Gln Gly Asp Pro Tyr Asp Glu Glu Thr Val Phe Gly Pro 370 375 380 Val Ala Ser Ala Ser Gln Tyr Ser Thr Val Met Ser Tyr Ile Asp Ser 385 390 395 400 Ala Arg Glu Glu Gly Ala Arg Val Val Ala Gly Gly Thr Arg Ser Ile 405 410 415 Ser Leu Ser Glu Gly Leu Glu Ser Gly Glu Phe Ile Gln Pro Thr Val 420 425 430 Phe Ala Asp Val Thr Pro Asp Met Arg Ile Ser Arg Glu Glu Ile Phe 435 440 445 Gly Pro Val Ile Ser Ile Leu Lys Tyr Asp Asp Thr Asn Gly Val Ser 450 455 460 Glu Ala Ile Ala Leu Ala Asn Asn Thr Lys Phe Gly Leu Gly Gly Leu 465 470 475 480 Val Phe Gly Ala Asp Glu Glu Gln Ala Leu Glu Val Ala Arg Gln Val 485 490 495 Asp Ser Gly Ser Val Gly Ile Asn Phe Phe Gly Ser Asn His Ser Ala 500 505 510 Pro Phe Gly Gly Arg His Glu Ser Gly Met Gly Val Glu Tyr Gly Ile 515 520 525 Glu Gly Leu Ser Ala Tyr Leu Thr Tyr Lys Ser Ile His Arg Thr Ile 530 535 540 <210> 31 <211> 1669 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1639) <223> RXA00772 <400> 31 ctgcgttaac gaattttgtc ggacgttaaa atggtcgcat tctgcttgct gaagtggcac 60 acctatgtgt tctgcttggg tatagcagtg cgggaaaaat ttg aaa aag tcc gat 115 Leu Lys Lys Ser Asp 1 5 tac ctg agg agg tat tca atg tct gat cgc att gct tca gaa aag ctg 163 Tyr Leu Arg Arg Tyr Ser Met Ser Asp Arg Ile Ala Ser Glu Lys Leu 10 15 20 cgc tcc aag ctc atg tcc gcc gac gag gcg gca cag ttt gtt aac cac 211 Arg Ser Lys Leu Met Ser Ala Asp Glu Ala Ala Gln Phe Val Asn His 25 30 35 ggt gac aag gtt ggt ttc tcc ggc ttc acc ggc gct ggc tac cca aag 259 Gly Asp Lys Val Gly Phe Ser Gly Phe Thr Gly Ala Gly Tyr Pro Lys 40 45 50 gca ctg cct acg gca atc gct aac cgg gct aaa gaa gca cac ggt gca 307 Ala Leu Pro Thr Ala Ile Ala Asn Arg Ala Lys Glu Ala His Gly Ala 55 60 65 ggc aac gac tac gca atc gac ctg ttc act ggc gca tcg acc gcc cct 355 Gly Asn Asp Tyr Ala Ile Asp Leu Phe Thr Gly Ala Ser Thr Ala Pro 70 75 80 85 gac tgc gat ggc gta ctt gca gaa gct gac gct atc cgc tgg cgc atg 403 Asp Cys Asp Gly Val Leu Ala Glu Ala Asp Ala Ile Arg Trp Arg Met 90 95 100 cca tac gca tct gat cca atc atg cgt aac aag atc aac tcc ggc tcc 451 Pro Tyr Ala Ser Asp Pro Ile Met Arg Asn Lys Ile Asn Ser Gly Ser 105 110 115 atg gga tac tcc gat atc cac ctg tcc cac tcc ggc cag cag gtt gaa 499 Met Gly Tyr Ser Asp Ile His Leu Ser His Ser Gly Gln Gln Val Glu 120 125 130 gag ggc ttc ttc ggc cag ctc aac gta gct gtc att gaa atc acc cgc 547 Glu Gly Phe Phe Gly Gln Leu Asn Val Ala Val Ile Glu Ile Thr Arg 135 140 145 atc act gaa gag ggc tac atc atc cct tct tcc tcc gtg ggt aac aac 595 Ile Thr Glu Glu Gly Tyr Ile Ile Pro Ser Ser Ser Val Gly Asn Asn 150 155 160 165 gtt gag tgg ctc aac gct gca gag aag gtc atc ctc gag gtt aac tct 643 Val Glu Trp Leu Asn Ala Ala Glu Lys Val Ile Leu Glu Val Asn Ser 170 175 180 tgg cag tct gaa gac ctc gaa ggt atg cac gac atc tgg tct gtt cct 691 Trp Gln Ser Glu Asp Leu Glu Gly Met His Asp Ile Trp Ser Val Pro 185 190 195 gcc ctg cca aac cgc att gcc gtg cca atc aac aag cca ggc gac cgc 739 Ala Leu Pro Asn Arg Ile Ala Val Pro Ile Asn Lys Pro Gly Asp Arg 200 205 210 atc ggt aag acc tac atc gag ttc gac acc gac aag gtt gtt gct gtt 787 Ile Gly Lys Thr Tyr Ile Glu Phe Asp Thr Asp Lys Val Val Ala Val 215 220 225 gtt gag acc aac acc gca gac cgc aac gca cca ttc aag cct gtc gac 835 Val Glu Thr Asn Thr Ala Asp Arg Asn Ala Pro Phe Lys Pro Val Asp 230 235 240 245 gac atc tct aag aag atc gct ggc aac ttc ctc gac ttc ctg gaa agc 883 Asp Ile Ser Lys Lys Ile Ala Gly Asn Phe Leu Asp Phe Leu Glu Ser 250 255 260 gaa gtt gct gca ggt cgc ctg tcc tac gac ggc tac atc atg cag tcc 931 Glu Val Ala Ala Gly Arg Leu Ser Tyr Asp Gly Tyr Ile Met Gln Ser 265 270 275 ggc gtg ggc aac gtg cca aac gcg gtg atg gca ggc ctg ctg gaa tcc 979 Gly Val Gly Asn Val Pro Asn Ala Val Met Ala Gly Leu Leu Glu Ser 280 285 290 aag ttt gag aac atc cag gcc tac acc gaa gtt atc cag gac ggc atg 1027 Lys Phe Glu Asn Ile Gln Ala Tyr Thr Glu Val Ile Gln Asp Gly Met 295 300 305 gtg gac ctc atc gac gcc ggc aag atg acc gtt gca tcc gca act tcc 1075 Val Asp Leu Ile Asp Ala Gly Lys Met Thr Val Ala Ser Ala Thr Ser 310 315 320 325 ttc tcc ctg tct cct gag tac gca gag aag atg aac aac gag gct aag 1123 Phe Ser Leu Ser Pro Glu Tyr Ala Glu Lys Met Asn Asn Glu Ala Lys 330 335 340 cgt tac cgc gag tcc att atc ctg cgc cca cag cag atc tct aac cac 1171 Arg Tyr Arg Glu Ser Ile Ile Leu Arg Pro Gln Gln Ile Ser Asn His 345 350 355 cca gag gtc atc cgc cgc gtt ggc ctg atc gcc acc aac ggt ctc atc 1219 Pro Glu Val Ile Arg Arg Val Gly Leu Ile Ala Thr Asn Gly Leu Ile 360 365 370 gag gct gac att tac ggc aac gtc aac tcc acc aac gtt tct ggc tcc 1267 Glu Ala Asp Ile Tyr Gly Asn Val Asn Ser Thr Asn Val Ser Gly Ser 375 380 385 cgc gtc atg aac ggc atc ggc ggc tcc ggc gac ttc acc cgt aac ggc 1315 Arg Val Met Asn Gly Ile Gly Gly Ser Gly Asp Phe Thr Arg Asn Gly 390 395 400 405 tac atc tcc agc ttc atc acc cct tca gag gca aag ggc ggc gca atc 1363 Tyr Ile Ser Ser Phe Ile Thr Pro Ser Glu Ala Lys Gly Gly Ala Ile 410 415 420 tct gcg atc gtt cct ttc gca tcc cac atc gac cac acc gag cac gat 1411 Ser Ala Ile Val Pro Phe Ala Ser His Ile Asp His Thr Glu His Asp 425 430 435 gtc atg gtt gtt atc tct gag tac ggt tac gca gac ctt cgt ggt ctg 1459 Val Met Val Val Ile Ser Glu Tyr Gly Tyr Ala Asp Leu Arg Gly Leu 440 445 450 gct cca cgt gag cgc gtt gcc aag atg atc ggc ctg gct cac cct gat 1507 Ala Pro Arg Glu Arg Val Ala Lys Met Ile Gly Leu Ala His Pro Asp 455 460 465 tac cgc cca ctg ctc gag gag tac tac gct cgc gca acc tcc ggt gac 1555 Tyr Arg Pro Leu Leu Glu Glu Tyr Tyr Ala Arg Ala Thr Ser Gly Asp 470 475 480 485 aac aag tac atg cag acc cct cat gat ctt gca acc gcg ttt gat ttc 1603 Asn Lys Tyr Met Gln Thr Pro His Asp Leu Ala Thr Ala Phe Asp Phe 490 495 500 cac atc aac ctg gct aag aac ggc tcc atg aag gca taagtttttt 1649 His Ile Asn Leu Ala Lys Asn Gly Ser Met Lys Ala 505 510 cttggtttag aaaccgccgc 1669 <210> 32 <211> 513 <212> PRT <213> Corynebacterium glutamicum <400> 32 Leu Lys Lys Ser Asp Tyr Leu Arg Arg Tyr Ser Met Ser Asp Arg Ile 1 5 10 15 Ala Ser Glu Lys Leu Arg Ser Lys Leu Met Ser Ala Asp Glu Ala Ala 20 25 30 Gln Phe Val Asn His Gly Asp Lys Val Gly Phe Ser Gly Phe Thr Gly 35 40 45 Ala Gly Tyr Pro Lys Ala Leu Pro Thr Ala Ile Ala Asn Arg Ala Lys 50 55 60 Glu Ala His Gly Ala Gly Asn Asp Tyr Ala Ile Asp Leu Phe Thr Gly 65 70 75 80 Ala Ser Thr Ala Pro Asp Cys Asp Gly Val Leu Ala Glu Ala Asp Ala 85 90 95 Ile Arg Trp Arg Met Pro Tyr Ala Ser Asp Pro Ile Met Arg Asn Lys 100 105 110 Ile Asn Ser Gly Ser Met Gly Tyr Ser Asp Ile His Leu Ser His Ser 115 120 125 Gly Gln Gln Val Glu Glu Gly Phe Phe Gly Gln Leu Asn Val Ala Val 130 135 140 Ile Glu Ile Thr Arg Ile Thr Glu Glu Gly Tyr Ile Ile Pro Ser Ser 145 150 155 160 Ser Val Gly Asn Asn Val Glu Trp Leu Asn Ala Ala Glu Lys Val Ile 165 170 175 Leu Glu Val Asn Ser Trp Gln Ser Glu Asp Leu Glu Gly Met His Asp 180 185 190 Ile Trp Ser Val Pro Ala Leu Pro Asn Arg Ile Ala Val Pro Ile Asn 195 200 205 Lys Pro Gly Asp Arg Ile Gly Lys Thr Tyr Ile Glu Phe Asp Thr Asp 210 215 220 Lys Val Val Ala Val Val Glu Thr Asn Thr Ala Asp Arg Asn Ala Pro 225 230 235 240 Phe Lys Pro Val Asp Asp Ile Ser Lys Lys Ile Ala Gly Asn Phe Leu 245 250 255 Asp Phe Leu Glu Ser Glu Val Ala Ala Gly Arg Leu Ser Tyr Asp Gly 260 265 270 Tyr Ile Met Gln Ser Gly Val Gly Asn Val Pro Asn Ala Val Met Ala 275 280 285 Gly Leu Leu Glu Ser Lys Phe Glu Asn Ile Gln Ala Tyr Thr Glu Val 290 295 300 Ile Gln Asp Gly Met Val Asp Leu Ile Asp Ala Gly Lys Met Thr Val 305 310 315 320 Ala Ser Ala Thr Ser Phe Ser Leu Ser Pro Glu Tyr Ala Glu Lys Met 325 330 335 Asn Asn Glu Ala Lys Arg Tyr Arg Glu Ser Ile Ile Leu Arg Pro Gln 340 345 350 Gln Ile Ser Asn His Pro Glu Val Ile Arg Arg Val Gly Leu Ile Ala 355 360 365 Thr Asn Gly Leu Ile Glu Ala Asp Ile Tyr Gly Asn Val Asn Ser Thr 370 375 380 Asn Val Ser Gly Ser Arg Val Met Asn Gly Ile Gly Gly Ser Gly Asp 385 390 395 400 Phe Thr Arg Asn Gly Tyr Ile Ser Ser Phe Ile Thr Pro Ser Glu Ala 405 410 415 Lys Gly Gly Ala Ile Ser Ala Ile Val Pro Phe Ala Ser His Ile Asp 420 425 430 His Thr Glu His Asp Val Met Val Val Ile Ser Glu Tyr Gly Tyr Ala 435 440 445 Asp Leu Arg Gly Leu Ala Pro Arg Glu Arg Val Ala Lys Met Ile Gly 450 455 460 Leu Ala His Pro Asp Tyr Arg Pro Leu Leu Glu Glu Tyr Tyr Ala Arg 465 470 475 480 Ala Thr Ser Gly Asp Asn Lys Tyr Met Gln Thr Pro His Asp Leu Ala 485 490 495 Thr Ala Phe Asp Phe His Ile Asn Leu Ala Lys Asn Gly Ser Met Lys 500 505 510 Ala <210> 33 <211> 1096 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1066) <223> RXA00818 <400> 33 tcggggcctt gcacctgaaa ttatggcata tcaaacgcca caagacaaca tcaaacccga 60 ttatttattc gaataacacg aatacttaac agtgtggcaa atg aca ctt ccc gca 115 Met thr leu pro ala 1 5 cgc ctt cta cat tcc caa tgc gca acc cca gca cgt cac ttc aat ctc 163 Arg Leu Leu His Ser Gln Cys Ala Thr Pro Ala Arg His Phe Asn Leu 10 15 20 att ggt aac ttt agt ttc ttt ctc agt ctt gga aag ttg cca aaa agc 211 Ile Gly Asn Phe Ser Phe Phe Leu Ser Leu Gly Lys Leu Pro Lys Ser 25 30 35 gct aaa cta tgc ggt gtg aag cac cca gat ccc gcc caa aaa gta gag 259 Ala Lys Leu Cys Gly Val Lys His Pro Asp Pro Ala Gln Lys Val Glu 40 45 50 ggc acc act gcg acc acc ccc aca aag gtg gct gct ttt ttc gat ctg 307 Gly Thr Thr Ala Thr Thr Pro Thr Lys Val Ala Ala Phe Phe Asp Leu 55 60 65 gac aag acc atc atc gcc atg agt tcc acc tac gcc tac ggc cgt gag 355 Asp Lys Thr Ile Ile Ala Met Ser Ser Thr Tyr Ala Tyr Gly Arg Glu 70 75 80 85 ttc atg aac agc ggg ctc atc tcc cct gtc gaa gcc ctg caa tta agc 403 Phe Met Asn Ser Gly Leu Ile Ser Pro Val Glu Ala Leu Gln Leu Ser 90 95 100 ctc gcg caa gca acg tac atg ttc gcc ggc cac acc agt gaa caa atg 451 Leu Ala Gln Ala Thr Tyr Met Phe Ala Gly His Thr Ser Glu Gln Met 105 110 115 gac aac acc cgc gac caa ctc acc gcc atg atc cgc ggc tgg gaa gtc 499 Asp Asn Thr Arg Asp Gln Leu Thr Ala Met Ile Arg Gly Trp Glu Val 120 125 130 caa cag gtg cgc tcg atc gcg gag gaa acc atg cat tcg gtg gtc act 547 Gln Gln Val Arg Ser Ile Ala Glu Glu Thr Met His Ser Val Val Thr 135 140 145 ccc acc atc tac gca gag gcc cgc gaa ctg atc gag cac cac cag gag 595 Pro Thr Ile Tyr Ala Glu Ala Arg Glu Leu Ile Glu His His Gln Glu 150 155 160 165 ctc ggc cac gat gtc atc atc att tcc gcc tct gtg aaa gaa ctg gtg 643 Leu Gly His Asp Val Ile Ile Ile Ser Ala Ser Val Lys Glu Leu Val 170 175 180 gaa ccc atc gcc cgc gaa ctg ggt gta cat aaa act gtc acc acc gtg 691 Glu Pro Ile Ala Arg Glu Leu Gly Val His Lys Thr Val Thr Thr Val 185 190 195 ctt gaa gcc cac gac ggt atg tac acc ggt gaa gtg ctg ttt tac tgc 739 Leu Glu Ala His Asp Gly Met Tyr Thr Gly Glu Val Leu Phe Tyr Cys 200 205 210 aaa ggc gac gct aaa gcg cag tcc atc ctg gat ctc gcc gag gcg aac 787 Lys Gly Asp Ala Lys Ala Gln Ser Ile Leu Asp Leu Ala Glu Ala Asn 215 220 225 aat tac gac ctt tcc tta agc ttc gcc tac tcc gat tcc ttc acg gac 835 Asn Tyr Asp Leu Ser Leu Ser Phe Ala Tyr Ser Asp Ser Phe Thr Asp 230 235 240 245 ctg ccc atg ttg gaa gct gtc ggc aac ccg gcc gcc gtc aac ccc gac 883 Leu Pro Met Leu Glu Ala Val Gly Asn Pro Ala Ala Val Asn Pro Asp 250 255 260 cgc gcg ctg aag aaa atc gcc ctt gaa cag ggg tgg aaa atc tta agc 931 Arg Ala Leu Lys Lys Ile Ala Leu Glu Gln Gly Trp Lys Ile Leu Ser 265 270 275 ttc aaa aac cct gaa ccg ctg ttc caa atg ccc agc acc cgc gac gtc 979 Phe Lys Asn Pro Glu Pro Leu Phe Gln Met Pro Ser Thr Arg Asp Val 280 285 290 ggc atc gga acc gga gtt gtt gcc ggc atc gca gct gtt aca gca ggt 1027 Gly Ile Gly Thr Gly Val Val Ala Gly Ile Ala Ala Val Thr Ala Gly 295 300 305 agt atc tgg tgg atg aaa cgc gca cgg cgc gga tcg gcc tgagcctcac 1076 Ser Ile Trp Trp Met Lys Arg Ala Arg Arg Gly Ser Ala 310 315 320 ctgacagcag ttagtggcat 1096 <210> 34 <211> 322 <212> PRT <213> Corynebacterium glutamicum <400> 34 Met Thr Leu Pro Ala Arg Leu Leu His Ser Gln Cys Ala Thr Pro Ala 1 5 10 15 Arg His Phe Asn Leu Ile Gly Asn Phe Ser Phe Phe Leu Ser Leu Gly 20 25 30 Lys Leu Pro Lys Ser Ala Lys Leu Cys Gly Val Lys His Pro Asp Pro 35 40 45 Ala Gln Lys Val Glu Gly Thr Thr Ala Thr Thr Pro Thr Lys Val Ala 50 55 60 Ala Phe Phe Asp Leu Asp Lys Thr Ile Ile Ala Met Ser Ser Thr Tyr 65 70 75 80 Ala Tyr Gly Arg Glu Phe Met Asn Ser Gly Leu Ile Ser Pro Val Glu 85 90 95 Ala Leu Gln Leu Ser Leu Ala Gln Ala Thr Tyr Met Phe Ala Gly His 100 105 110 Thr Ser Glu Gln Met Asp Asn Thr Arg Asp Gln Leu Thr Ala Met Ile 115 120 125 Arg Gly Trp Glu Val Gln Gln Val Arg Ser Ile Ala Glu Glu Thr Met 130 135 140 His Ser Val Val Thr Pro Thr Ile Tyr Ala Glu Ala Arg Glu Leu Ile 145 150 155 160 Glu His His Gln Glu Leu Gly His Asp Val Ile Ile Ile Ser Ala Ser 165 170 175 Val Lys Glu Leu Val Glu Pro Ile Ala Arg Glu Leu Gly Val His Lys 180 185 190 Thr Val Thr Thr Val Leu Glu Ala His Asp Gly Met Tyr Thr Gly Glu 195 200 205 Val Leu Phe Tyr Cys Lys Gly Asp Ala Lys Ala Gln Ser Ile Leu Asp 210 215 220 Leu Ala Glu Ala Asn Asn Tyr Asp Leu Ser Leu Ser Phe Ala Tyr Ser 225 230 235 240 Asp Ser Phe Thr Asp Leu Pro Met Leu Glu Ala Val Gly Asn Pro Ala 245 250 255 Ala Val Asn Pro Asp Arg Ala Leu Lys Lys Ile Ala Leu Glu Gln Gly 260 265 270 Trp Lys Ile Leu Ser Phe Lys Asn Pro Glu Pro Leu Phe Gln Met Pro 275 280 285 Ser Thr Arg Asp Val Gly Ile Gly Thr Gly Val Val Ala Gly Ile Ala 290 295 300 Ala Val Thr Ala Gly Ser Ile Trp Trp Met Lys Arg Ala Arg Arg Gly 305 310 315 320 Ser ala <210> 35 <211> 964 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (934) <223> RXA00844 <400> 35 ggtgacggcc aaaggcgcgc gcattgcgcg ccccaaaatc gacgtcattg acagcatctt 60 gtgacaacat tttgtagagc aaccatctag actgttcttt atg tct tct gcg tca 115 Met Ser Ser Ala Ser 1 5 ttt acc acc aaa gca ctg tcc gta ctc gca gct tta acg gct gcg tct 163 Phe Thr Thr Lys Ala Leu Ser Val Leu Ala Ala Leu Thr Ala Ala Ser 10 15 20 gcc ccc tta gtg gcg gcg tca cct gca cat gct ttg gca aat gct cgc 211 Ala Pro Leu Val Ala Ala Ser Pro Ala His Ala Leu Ala Asn Ala Arg 25 30 35 aac gtt acg ggt tca agc acc act tca gat tca att gtt cgt ctg cac 259 Asn Val Thr Gly Ser Ser Thr Thr Ser Asp Ser Ile Val Arg Leu His 40 45 50 atc ggt aac act gca tgt aca gga acc atg atc acc cca acg tgg gcg 307 Ile Gly Asn Thr Ala Cys Thr Gly Thr Met Ile Thr Pro Thr Trp Ala 55 60 65 atc acc gcc cgc cac tgt atc cct gag ggc ggt att gcc ggt gca gct 355 Ile Thr Ala Arg His Cys Ile Pro Glu Gly Gly Ile Ala Gly Ala Ala 70 75 80 85 att ggt tca agc act ttg agc caa ttt cag cag gtg tcc caa gcg atc 403 Ile Gly Ser Ser Thr Leu Ser Gln Phe Gln Gln Val Ser Gln Ala Ile 90 95 100 ttg cac cct act gcg gac tta gct ctc gtt gag ctt ccc aat cag gca 451 Leu His Pro Thr Ala Asp Leu Ala Leu Val Glu Leu Pro Asn Gln Ala 105 110 115 agt tcc aac acg gtt gat ctc tac ggt gca cac gtg cag cct ggt gaa 499 Ser Ser Asn Thr Val Asp Leu Tyr Gly Ala His Val Gln Pro Gly Glu 120 125 130 aat ggt caa gca gcc ggc tgg ggt ggg tac tct gcc ttt ggc caa aat 547 Asn Gly Gln Ala Ala Gly Trp Gly Gly Tyr Ser Ala Phe Gly Gln Asn 135 140 145 gtt gca cag caa gcc gat gtg caa att caa cgc agg gta gtc aat gtg 595 Val Ala Gln Gln Ala Asp Val Gln Ile Gln Arg Arg Val Val Asn Val 150 155 160 165 cca agc ccc gac cgc acc gct gtg ctg ctt gaa ggc act gtt tct aac 643 Pro Ser Pro Asp Arg Thr Ala Val Leu Leu Glu Gly Thr Val Ser Asn 170 175 180 ggt cgt ctc gta cca ggc gat tcc ggc gga cct ttg tac atc aat ggt 691 Gly Arg Leu Val Pro Gly Asp Ser Gly Gly Pro Leu Tyr Ile Asn Gly 185 190 195 caa ctg gct ggt gtg ctc agc atg tcc act gac gta gaa aac gat gca 739 Gln Leu Ala Gly Val Leu Ser Met Ser Thr Asp Val Glu Asn Asp Ala 200 205 210 cta gac ggc acc gtc ggc tgg tac atc ccc gtt gct gaa cac gcc gag 787 Leu Asp Gly Thr Val Gly Trp Tyr Ile Pro Val Ala Glu His Ala Glu 215 220 225 tgg atc gcc tac tac acc ggc aag cac att gcc ccc att gct ggt gcg 835 Trp Ile Ala Tyr Tyr Thr Gly Lys His Ile Ala Pro Ile Ala Gly Ala 230 235 240 245 ccc gca gaa ctt gtt gac gcc acc gcc aac ccc acc ttc atc cct gct 883 Pro Ala Glu Leu Val Asp Ala Thr Ala Asn Pro Thr Phe Ile Pro Ala 250 255 260 cca cag cct ttc acc ggt tca tcc atc ggt ggt tgg gcg ctg ggc agc 931 Pro Gln Pro Phe Thr Gly Ser Ser Ile Gly Gly Trp Ala Leu Gly Ser 265 270 275 tcc tagaatatgc tgatctccct gctgatagtc 964 Ser <210> 36 <211> 278 <212> PRT <213> Corynebacterium glutamicum <400> 36 Met Ser Ser Ala Ser Phe Thr Thr Lys Ala Leu Ser Val Leu Ala Ala 1 5 10 15 Leu Thr Ala Ala Ser Ala Pro Leu Val Ala Ala Ser Pro Ala His Ala 20 25 30 Leu Ala Asn Ala Arg Asn Val Thr Gly Ser Ser Thr Thr Ser Asp Ser 35 40 45 Ile Val Arg Leu His Ile Gly Asn Thr Ala Cys Thr Gly Thr Met Ile 50 55 60 Thr Pro Thr Trp Ala Ile Thr Ala Arg His Cys Ile Pro Glu Gly Gly 65 70 75 80 Ile Ala Gly Ala Ala Ile Gly Ser Ser Thr Leu Ser Gln Phe Gln Gln 85 90 95 Val Ser Gln Ala Ile Leu His Pro Thr Ala Asp Leu Ala Leu Val Glu 100 105 110 Leu Pro Asn Gln Ala Ser Ser Asn Thr Val Asp Leu Tyr Gly Ala His 115 120 125 Val Gln Pro Gly Glu Asn Gly Gln Ala Ala Gly Trp Gly Gly Tyr Ser 130 135 140 Ala Phe Gly Gln Asn Val Ala Gln Gln Ala Asp Val Gln Ile Gln Arg 145 150 155 160 Arg Val Val Asn Val Pro Ser Pro Asp Arg Thr Ala Val Leu Leu Glu 165 170 175 Gly Thr Val Ser Asn Gly Arg Leu Val Pro Gly Asp Ser Gly Gly Pro 180 185 190 Leu Tyr Ile Asn Gly Gln Leu Ala Gly Val Leu Ser Met Ser Thr Asp 195 200 205 Val Glu Asn Asp Ala Leu Asp Gly Thr Val Gly Trp Tyr Ile Pro Val 210 215 220 Ala Glu His Ala Glu Trp Ile Ala Tyr Tyr Thr Gly Lys His Ile Ala 225 230 235 240 Pro Ile Ala Gly Ala Pro Ala Glu Leu Val Asp Ala Thr Ala Asn Pro 245 250 255 Thr Phe Ile Pro Ala Pro Gln Pro Phe Thr Gly Ser Ser Ile Gly Gly 260 265 270 Trp Ala Leu Gly Ser Ser 275 <210> 37 <211> 1180 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (1150) <223> RXA00888 <400> 37 ggccctgagg tgttgcgcac tgcctccgca ggcatggtgg cgctcgcagc tatcggcgtg 60 ctgtctgacc gctggtaaca tttctctgac ataacctttc ttg aac att ccg aaa 115 Leu Asn Ile Pro Lys 1 5 gca ggc cga act agc act gtg act gcc ccg cga aaa acc cgc aca gaa 163 Ala Gly Arg Thr Ser Thr Val Thr Ala Pro Arg Lys Thr Arg Thr Glu 10 15 20 gta atc acc act gtc ctc aat cta gaa cgc acg ctc gcg caa acc gtt 211 Val Ile Thr Thr Val Leu Asn Leu Glu Arg Thr Leu Ala Gln Thr Val 25 30 35 tta gga atc aac gac gaa aac ctg cgt gtg ttg gac aat caa att gat 259 Leu Gly Ile Asn Asp Glu Asn Leu Arg Val Leu Asp Asn Gln Ile Asp 40 45 50 tgc gat att cac gtg cgt ggc acc cac gtg gaa ctc acc ggg cca gcc 307 Cys Asp Ile His Val Arg Gly Thr His Val Glu Leu Thr Gly Pro Ala 55 60 65 cac gaa gtc tcc cgc gcc tcg aaa ata ttt gag gaa ctg cag gcg att 355 His Glu Val Ser Arg Ala Ser Lys Ile Phe Glu Glu Leu Gln Ala Ile 70 75 80 85 gcc cgt cga gga cat gtg att agc cct gag aca gta aaa aat gtc atc 403 Ala Arg Arg Gly His Val Ile Ser Pro Glu Thr Val Lys Asn Val Ile 90 95 100 agc atg att aac gtg gag acg ccg caa acc gtc tct gaa atc ttg acc 451 Ser Met Ile Asn Val Glu Thr Pro Gln Thr Val Ser Glu Ile Leu Thr 105 110 115 ggc gat atc ctt gct cgc cgt ggc aaa gtg atc cgc cct aag acg ctt 499 Gly Asp Ile Leu Ala Arg Arg Gly Lys Val Ile Arg Pro Lys Thr Leu 120 125 130 ggc caa aag cac tac gtg gac gcg att gat acc aac acg att gtg ttc 547 Gly Gln Lys His Tyr Val Asp Ala Ile Asp Thr Asn Thr Ile Val Phe 135 140 145 ggt ctg ggc cca gcc ggt tcc ggt aaa acc tat ctg gcc atg gca aaa 595 Gly Leu Gly Pro Ala Gly Ser Gly Lys Thr Tyr Leu Ala Met Ala Lys 150 155 160 165 gcc gtc caa gcg ctg caa tca aag cag gtc agc cgc atc atc ttg acc 643 Ala Val Gln Ala Leu Gln Ser Lys Gln Val Ser Arg Ile Ile Leu Thr 170 175 180 cgc ccc gca gtg gaa gcc ggc gag aaa ctc ggc ttc ttg ccc ggc acc 691 Arg Pro Ala Val Glu Ala Gly Glu Lys Leu Gly Phe Leu Pro Gly Thr 185 190 195 ctg aac gaa aag atc gac ccc tac ctg cgc ccg ctt cac gac gcg ctg 739 Leu Asn Glu Lys Ile Asp Pro Tyr Leu Arg Pro Leu His Asp Ala Leu 200 205 210 cgc gac atg gtc gaa cca gaa gtc att cca aaa ctc atg gaa gcc ggc 787 Arg Asp Met Val Glu Pro Glu Val Ile Pro Lys Leu Met Glu Ala Gly 215 220 225 atc gta gaa gtc gcc cca ctt gcc tac atg cgc gga cgc acc ctc aac 835 Ile Val Glu Val Ala Pro Leu Ala Tyr Met Arg Gly Arg Thr Leu Asn 230 235 240 245 gat gca ttc gtg atc ctg gat gaa gcc cag aac acc acc cca gca cag 883 Asp Ala Phe Val Ile Leu Asp Glu Ala Gln Asn Thr Thr Pro Ala Gln 250 255 260 atg aag atg ttc ctc acc cgc ctg gga ttc ggt tcc aaa atg gta gtc 931 Met Lys Met Phe Leu Thr Arg Leu Gly Phe Gly Ser Lys Met Val Val 265 270 275 aca ggt gac atc acc cag gtg gac ctc cca gga ggc caa aag tcc ggc 979 Thr Gly Asp Ile Thr Gln Val Asp Leu Pro Gly Gly Gln Lys Ser Gly 280 285 290 ctg cgc ctg gtt cgc cac atc ctg cgc gga gta gac gat gtg cac ttc 1027 Leu Arg Leu Val Arg His Ile Leu Arg Gly Val Asp Asp Val His Phe 295 300 305 tcc gag ctc acc tca tcc gac gtg gtc cgc cac caa ctg gtt gga cac 1075 Ser Glu Leu Thr Ser Ser Asp Val Val Arg His Gln Leu Val Gly His 310 315 320 325 att gtg gat gca tac gaa gac tat gaa gaa cgc gag gcc cgc gaa ttg 1123 Ile Val Asp Ala Tyr Glu Asp Tyr Glu Glu Arg Glu Ala Arg Glu Leu 330 335 340 aaa cgc aaa cgc cag gag aca cgg cca tgagcattga ggtattcaac 1170 Lys Arg Lys Arg Gln Glu Thr Arg Pro 345 350 gaatcaggat 1180 <210> 38 <211> 350 <212> PRT <213> Corynebacterium glutamicum <400> 38 Leu Asn Ile Pro Lys Ala Gly Arg Thr Ser Thr Val Thr Ala Pro Arg 1 5 10 15 Lys Thr Arg Thr Glu Val Ile Thr Thr Val Leu Asn Leu Glu Arg Thr 20 25 30 Leu Ala Gln Thr Val Leu Gly Ile Asn Asp Glu Asn Leu Arg Val Leu 35 40 45 Asp Asn Gln Ile Asp Cys Asp Ile His Val Arg Gly Thr His Val Glu 50 55 60 Leu Thr Gly Pro Ala His Glu Val Ser Arg Ala Ser Lys Ile Phe Glu 65 70 75 80 Glu Leu Gln Ala Ile Ala Arg Arg Gly His Val Ile Ser Pro Glu Thr 85 90 95 Val Lys Asn Val Ile Ser Met Ile Asn Val Glu Thr Pro Gln Thr Val 100 105 110 Ser Glu Ile Leu Thr Gly Asp Ile Leu Ala Arg Arg Gly Lys Val Ile 115 120 125 Arg Pro Lys Thr Leu Gly Gln Lys His Tyr Val Asp Ala Ile Asp Thr 130 135 140 Asn Thr Ile Val Phe Gly Leu Gly Pro Ala Gly Ser Gly Lys Thr Tyr 145 150 155 160 Leu Ala Met Ala Lys Ala Val Gln Ala Leu Gln Ser Lys Gln Val Ser 165 170 175 Arg Ile Ile Leu Thr Arg Pro Ala Val Glu Ala Gly Glu Lys Leu Gly 180 185 190 Phe Leu Pro Gly Thr Leu Asn Glu Lys Ile Asp Pro Tyr Leu Arg Pro 195 200 205 Leu His Asp Ala Leu Arg Asp Met Val Glu Pro Glu Val Ile Pro Lys 210 215 220 Leu Met Glu Ala Gly Ile Val Glu Val Ala Pro Leu Ala Tyr Met Arg 225 230 235 240 Gly Arg Thr Leu Asn Asp Ala Phe Val Ile Leu Asp Glu Ala Gln Asn 245 250 255 Thr Thr Pro Ala Gln Met Lys Met Phe Leu Thr Arg Leu Gly Phe Gly 260 265 270 Ser Lys Met Val Val Thr Gly Asp Ile Thr Gln Val Asp Leu Pro Gly 275 280 285 Gly Gln Lys Ser Gly Leu Arg Leu Val Arg His Ile Leu Arg Gly Val 290 295 300 Asp Asp Val His Phe Ser Glu Leu Thr Ser Ser Asp Val Val Arg His 305 310 315 320 Gln Leu Val Gly His Ile Val Asp Ala Tyr Glu Asp Tyr Glu Glu Arg 325 330 335 Glu Ala Arg Glu Leu Lys Arg Lys Arg Gln Glu Thr Arg Pro 340 345 350 <210> 39 <211> 1309 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1279) <223> RXA00892 <400> 39 ttgatcgctg cgaaatcggg cagctggtag ggcaaggtgc tgggcttgag caggtgttca 60 acagtcatag tgaccgatac tagttgctag agttttcatc atg tct gcc acc tct 115 Met Ser Ala Thr Ser 1 5 tcc gtc acc gtt gaa tgc ccg gcg gga acc atc acc ggc gag ccc cat 163 Ser Val Thr Val Glu Cys Pro Ala Gly Thr Ile Thr Gly Glu Pro His 10 15 20 tat ttc cgc tcg att ccc tac gca aag gcg cgc ccg ttt gct gat gcc 211 Tyr Phe Arg Ser Ile Pro Tyr Ala Lys Ala Arg Pro Phe Ala Asp Ala 25 30 35 gaa aag cta gaa ccc ctg cgc att gat gcg acc ggc aag cac gag ggt 259 Glu Lys Leu Glu Pro Leu Arg Ile Asp Ala Thr Gly Lys His Glu Gly 40 45 50 ctc tat tta acg ttg gca acc ccg gag gca cgg ttc ggc gcg gat gcc 307 Leu Tyr Leu Thr Leu Ala Thr Pro Glu Ala Arg Phe Gly Ala Asp Ala 55 60 65 ccg gtg atc gtt tat atc cac ggc gga gga tat gac ggt ggc acg cgt 355 Pro Val Ile Val Tyr Ile His Gly Gly Gly Tyr Asp Gly Gly Thr Arg 70 75 80 85 ttc gat gcc cgc acc gag ccc act ttc ttc cgt gag cag ggc ttt gtg 403 Phe Asp Ala Arg Thr Glu Pro Thr Phe Phe Arg Glu Gln Gly Phe Val 90 95 100 gtg gtc tcc atc gat tac cgc gtc ggc ctg gag ggc ttt gcg cgc ttc 451 Val Val Ser Ile Asp Tyr Arg Val Gly Leu Glu Gly Phe Ala Arg Phe 105 110 115 cac gac gac gag gcc aat cgt tac cgc ggc atc gat gat tgc gtg ctc 499 His Asp Asp Glu Ala Asn Arg Tyr Arg Gly Ile Asp Asp Cys Val Leu 120 125 130 gcg ctg gag tgg gtg cag aaa aac atc gag cat ttc ggc ggc gat ccc 547 Ala Leu Glu Trp Val Gln Lys Asn Ile Glu His Phe Gly Gly Asp Pro 135 140 145 acc aac gtc acc ctc atc ggg cag tcc gcg ggc gcc ggc atc gcg ctc 595 Thr Asn Val Thr Leu Ile Gly Gln Ser Ala Gly Ala Gly Ile Ala Leu 150 155 160 165 tgg ctc acg cgc cta gac cac tat aaa ggt gct ttt cga cgc ctg gtt 643 Trp Leu Thr Arg Leu Asp His Tyr Lys Gly Ala Phe Arg Arg Leu Val 170 175 180 gcg ctc tcc ccc agc ttc ccg cgc cag cca ttc gct gcc cga aaa ggt 691 Ala Leu Ser Pro Ser Phe Pro Arg Gln Pro Phe Ala Ala Arg Lys Gly 185 190 195 gct tta cga cgc gcc ctg ggc aaa ccc gtc acc cgc gcc tcg ctg gcg 739 Ala Leu Arg Arg Ala Leu Gly Lys Pro Val Thr Arg Ala Ser Leu Ala 200 205 210 ggc atc aag ccc gca cgc ttg gac aaa ggc tac cgt cgc ttt gct cgc 787 Gly Ile Lys Pro Ala Arg Leu Asp Lys Gly Tyr Arg Arg Phe Ala Arg 215 220 225 cgc tac ttc acc gac ctg gca ctc ggc cct acc cca tac gac ccg aat 835 Arg Tyr Phe Thr Asp Leu Ala Leu Gly Pro Thr Pro Tyr Asp Pro Asn 230 235 240 245 gag ctg gcc gac atc gat ttg atc atc tcc tcc acc cgc gat gaa atg 883 Glu Leu Ala Asp Ile Asp Leu Ile Ile Ser Ser Thr Arg Asp Glu Met 250 255 260 tac gga cac cgc gca ggc tta tgg ttc gac cag cgt ggc ttc ggc gca 931 Tyr Gly His Arg Ala Gly Leu Trp Phe Asp Gln Arg Gly Phe Gly Ala 265 270 275 aaa ctg gcc gcg cga ctt ttt ggc ctg gag aat tcg gat acc tat att 979 Lys Leu Ala Ala Arg Leu Phe Gly Leu Glu Asn Ser Asp Thr Tyr Ile 280 285 290 aag gaa gcc cgc aag atc gac aac cgc gtt gta ggc cgc atg att ggt 1027 Lys Glu Ala Arg Lys Ile Asp Asn Arg Val Val Gly Arg Met Ile Gly 295 300 305 gat gcc gtg atc cgc cgc tac gtc gcg caa acc gaa aaa ggt tgg tgg 1075 Asp Ala Val Ile Arg Arg Tyr Val Ala Gln Thr Glu Lys Gly Trp Trp 310 315 320 325 atg gaa ttt ccc ggc aag cac tgc gat gac ctg tcc ttg gtc ttc ttg 1123 Met Glu Phe Pro Gly Lys His Cys Asp Asp Leu Ser Leu Val Phe Leu 330 335 340 gaa gat tcc gaa gca cat cac cac atc gcc tcc ttc gcc cgc ggg gct 1171 Glu Asp Ser Glu Ala His His His Ile Ala Ser Phe Ala Arg Gly Ala 345 350 355 acg cca tcg tgg cct caa tat tcc gaa gaa aac cgc gca gct ctc tca 1219 Thr Pro Ser Trp Pro Gln Tyr Ser Glu Glu Asn Arg Ala Ala Leu Ser 360 365 370 cta gtc tca ggt gag gcg aaa gtg gtg aaa gac ccg cta cgc atg gtg 1267 Leu Val Ser Gly Glu Ala Lys Val Val Lys Asp Pro Leu Arg Met Val 375 380 385 cgc ctg gct ttt tagaatgtgc tgcaaacctc ctggcatttc 1309 Arg Leu Ala Phe 390 <210> 40 <211> 393 <212> PRT <213> Corynebacterium glutamicum <400> 40 Met Ser Ala Thr Ser Ser Val Thr Val Glu Cys Pro Ala Gly Thr Ile 1 5 10 15 Thr Gly Glu Pro His Tyr Phe Arg Ser Ile Pro Tyr Ala Lys Ala Arg 20 25 30 Pro Phe Ala Asp Ala Glu Lys Leu Glu Pro Leu Arg Ile Asp Ala Thr 35 40 45 Gly Lys His Glu Gly Leu Tyr Leu Thr Leu Ala Thr Pro Glu Ala Arg 50 55 60 Phe Gly Ala Asp Ala Pro Val Ile Val Tyr Ile His Gly Gly Gly Tyr 65 70 75 80 Asp Gly Gly Thr Arg Phe Asp Ala Arg Thr Glu Pro Thr Phe Phe Arg 85 90 95 Glu Gln Gly Phe Val Val Val Ser Ile Asp Tyr Arg Val Gly Leu Glu 100 105 110 Gly Phe Ala Arg Phe His Asp Asp Glu Ala Asn Arg Tyr Arg Gly Ile 115 120 125 Asp Asp Cys Val Leu Ala Leu Glu Trp Val Gln Lys Asn Ile Glu His 130 135 140 Phe Gly Gly Asp Pro Thr Asn Val Thr Leu Ile Gly Gln Ser Ala Gly 145 150 155 160 Ala Gly Ile Ala Leu Trp Leu Thr Arg Leu Asp His Tyr Lys Gly Ala 165 170 175 Phe Arg Arg Leu Val Ala Leu Ser Pro Ser Phe Pro Arg Gln Pro Phe 180 185 190 Ala Ala Arg Lys Gly Ala Leu Arg Arg Ala Leu Gly Lys Pro Val Thr 195 200 205 Arg Ala Ser Leu Ala Gly Ile Lys Pro Ala Arg Leu Asp Lys Gly Tyr 210 215 220 Arg Arg Phe Ala Arg Arg Tyr Phe Thr Asp Leu Ala Leu Gly Pro Thr 225 230 235 240 Pro Tyr Asp Pro Asn Glu Leu Ala Asp Ile Asp Leu Ile Ile Ser Ser 245 250 255 Thr Arg Asp Glu Met Tyr Gly His Arg Ala Gly Leu Trp Phe Asp Gln 260 265 270 Arg Gly Phe Gly Ala Lys Leu Ala Ala Arg Leu Phe Gly Leu Glu Asn 275 280 285 Ser Asp Thr Tyr Ile Lys Glu Ala Arg Lys Ile Asp Asn Arg Val Val 290 295 300 Gly Arg Met Ile Gly Asp Ala Val Ile Arg Arg Tyr Val Ala Gln Thr 305 310 315 320 Glu Lys Gly Trp Trp Met Glu Phe Pro Gly Lys His Cys Asp Asp Leu 325 330 335 Ser Leu Val Phe Leu Glu Asp Ser Glu Ala His His His Ile Ala Ser 340 345 350 Phe Ala Arg Gly Ala Thr Pro Ser Trp Pro Gln Tyr Ser Glu Glu Asn 355 360 365 Arg Ala Ala Leu Ser Leu Val Ser Gly Glu Ala Lys Val Val Lys Asp 370 375 380 Pro Leu Arg Met Val Arg Leu Ala Phe 385 390 <210> 41 <211> 967 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (937) <223> RXA00963 <400> 41 ctggctctga cggcgtcgac ttgttctgct tctctgatac accaattttc gaggccctta 60 acctcgcacg tacttttact ccggaaggaa tctagaactt atg cgt ctt gca aca 115 Met Arg Leu Ala Thr 1 5 atc cgc acc aac ggc acc acc att gct gct cgt gtt gaa tct gaa aac 163 Ile Arg Thr Asn Gly Thr Thr Ile Ala Ala Arg Val Glu Ser Glu Asn 10 15 20 acc gct acc acc atc gag ggc ttt gcc aac gtc ggt gaa tta ctc cag 211 Thr Ala Thr Thr Ile Glu Gly Phe Ala Asn Val Gly Glu Leu Leu Gln 25 30 35 gaa tcc aac tgg cgc gag ctg gca gaa aac gct gct ggt gag gct gtg 259 Glu Ser Asn Trp Arg Glu Leu Ala Glu Asn Ala Ala Gly Glu Ala Val 40 45 50 acc ttt gaa aac aag gag cta gat gca gta gtt cca gca cct aag aag 307 Thr Phe Glu Asn Lys Glu Leu Asp Ala Val Val Pro Ala Pro Lys Lys 55 60 65 att gtg tgc gtc ggc ctt aac tac gcc aac cac att aaa gaa atg ggc 355 Ile Val Cys Val Gly Leu Asn Tyr Ala Asn His Ile Lys Glu Met Gly 70 75 80 85 cgc gac ctc cct gat acc cca acc ctt ttt gtt aag ttc cct gac gcg 403 Arg Asp Leu Pro Asp Thr Pro Thr Leu Phe Val Lys Phe Pro Asp Ala 90 95 100 ctc atc gga cct ttc gat gat gtt gtc gtt cca gag tgg gct aac aag 451 Leu Ile Gly Pro Phe Asp Asp Val Val Val Pro Glu Trp Ala Asn Lys 105 110 115 gct ctc gac tgg gaa ggc gag atg gca gtt atc att ggc aag cgc gca 499 Ala Leu Asp Trp Glu Gly Glu Met Ala Val Ile Ile Gly Lys Arg Ala 120 125 130 cgc cgt gtc aag cag gcc gat gct gct gag tac atc gct ggc tac gca 547 Arg Arg Val Lys Gln Ala Asp Ala Ala Glu Tyr Ile Ala Gly Tyr Ala 135 140 145 gtg atg aac gat tac acc acc cgc gat ttc cag tac gca gca cct gca 595 Val Met Asn Asp Tyr Thr Thr Arg Asp Phe Gln Tyr Ala Ala Pro Ala 150 155 160 165 aag act cca cag tgg cac cag ggc aag tct ttg gaa aag tcc gct ggc 643 Lys Thr Pro Gln Trp His Gln Gly Lys Ser Leu Glu Lys Ser Ala Gly 170 175 180 ttc ggg cct tgg atg act acc cca gat tct ttt gag ttc ggc ggc gag 691 Phe Gly Pro Trp Met Thr Thr Pro Asp Ser Phe Glu Phe Gly Gly Glu 185 190 195 ctg gca acc tac ctc gag ggc gag aag gta cag tcc acc cct acc aat 739 Leu Ala Thr Tyr Leu Glu Gly Glu Lys Val Gln Ser Thr Pro Thr Asn 200 205 210 gac ctg gtc ttt agc cca gaa aag ctc atc gaa tac atc acc cac atc 787 Asp Leu Val Phe Ser Pro Glu Lys Leu Ile Glu Tyr Ile Thr His Ile 215 220 225 tac cca ttg gat gct ggc gac gtc att gtc acc ggt acc cca ggc ggc 835 Tyr Pro Leu Asp Ala Gly Asp Val Ile Val Thr Gly Thr Pro Gly Gly 230 235 240 245 gtt ggc cac gca cgt aac cca cag cgc tac atc ggt gac ggc gaa acc 883 Val Gly His Ala Arg Asn Pro Gln Arg Tyr Ile Gly Asp Gly Glu Thr 250 255 260 gta aag gtt gag att gcg ggc ctc ggc ttc att gaa aac aag acg gtg 931 Val Lys Val Glu Ile Ala Gly Leu Gly Phe Ile Glu Asn Lys Thr Val 265 270 275 ttt gaa taaatgacaa ctttccacga tcttccgctg 967 Phe glu <210> 42 <211> 279 <212> PRT <213> Corynebacterium glutamicum <400> 42 Met Arg Leu Ala Thr Ile Arg Thr Asn Gly Thr Thr Ile Ala Ala Arg 1 5 10 15 Val Glu Ser Glu Asn Thr Ala Thr Thr Ile Glu Gly Phe Ala Asn Val 20 25 30 Gly Glu Leu Leu Gln Glu Ser Asn Trp Arg Glu Leu Ala Glu Asn Ala 35 40 45 Ala Gly Glu Ala Val Thr Phe Glu Asn Lys Glu Leu Asp Ala Val Val 50 55 60 Pro Ala Pro Lys Lys Ile Val Cys Val Gly Leu Asn Tyr Ala Asn His 65 70 75 80 Ile Lys Glu Met Gly Arg Asp Leu Pro Asp Thr Pro Thr Leu Phe Val 85 90 95 Lys Phe Pro Asp Ala Leu Ile Gly Pro Phe Asp Asp Val Val Val Pro 100 105 110 Glu Trp Ala Asn Lys Ala Leu Asp Trp Glu Gly Glu Met Ala Val Ile 115 120 125 Ile Gly Lys Arg Ala Arg Arg Val Lys Gln Ala Asp Ala Ala Glu Tyr 130 135 140 Ile Ala Gly Tyr Ala Val Met Asn Asp Tyr Thr Thr Arg Asp Phe Gln 145 150 155 160 Tyr Ala Ala Pro Ala Lys Thr Pro Gln Trp His Gln Gly Lys Ser Leu 165 170 175 Glu Lys Ser Ala Gly Phe Gly Pro Trp Met Thr Thr Pro Asp Ser Phe 180 185 190 Glu Phe Gly Gly Glu Leu Ala Thr Tyr Leu Glu Gly Glu Lys Val Gln 195 200 205 Ser Thr Pro Thr Asn Asp Leu Val Phe Ser Pro Glu Lys Leu Ile Glu 210 215 220 Tyr Ile Thr His Ile Tyr Pro Leu Asp Ala Gly Asp Val Ile Val Thr 225 230 235 240 Gly Thr Pro Gly Gly Val Gly His Ala Arg Asn Pro Gln Arg Tyr Ile 245 250 255 Gly Asp Gly Glu Thr Val Lys Val Glu Ile Ala Gly Leu Gly Phe Ile 260 265 270 Glu Asn Lys Thr Val Phe Glu 275 <210> 43 <211> 1711 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1681) <223> RXA00982 <400> 43 tacttataac aaatccctgc ctttttaatg aaaaatcatt ggtctctgta tatgcttttt 60 ggggtaactg acccagaaaa caaacgtcct tggagccgta atg ccc cgt tcg aca 115 Met Pro Arg Ser Thr 1 5 ata aaa agg gta gta gca gtt ctt gcc gcc tcg act gcg ctt agc ccc 163 Ile Lys Arg Val Val Ala Val Leu Ala Ala Ser Thr Ala Leu Ser Pro 10 15 20 ttt ttg gta tca atg ccc act gca gca gcg caa gaa aac atc cgc tgg 211 Phe Leu Val Ser Met Pro Thr Ala Ala Ala Gln Glu Asn Ile Arg Trp 25 30 35 gaa gaa tgc cca cct cag gta gat att gcc tcc gct caa tgt ggc agc 259 Glu Glu Cys Pro Pro Gln Val Asp Ile Ala Ser Ala Gln Cys Gly Ser 40 45 50 atc gac gtg ccc atg cac tat tct gat ccc tca ctt ggc gat atc agc 307 Ile Asp Val Pro Met His Tyr Ser Asp Pro Ser Leu Gly Asp Ile Ser 55 60 65 gtg ggc ttt gtc aag gtc cct gcc caa ggc gaa aag cac ggc acc atc 355 Val Gly Phe Val Lys Val Pro Ala Gln Gly Glu Lys His Gly Thr Ile 70 75 80 85 ttc ggt aac tcc ggt ggc cct ggt ggc gat gcc tat agc ttc ttc ggc 403 Phe Gly Asn Ser Gly Gly Pro Gly Gly Asp Ala Tyr Ser Phe Phe Gly 90 95 100 agc caa tcc atg aac tgg cca gaa gcc atg tac caa aac tac gac ctc 451 Ser Gln Ser Met Asn Trp Pro Glu Ala Met Tyr Gln Asn Tyr Asp Leu 105 110 115 gtt gca gtg cag cct cgc gga atg gtc ggc tcc aca ccg gtt aac tgc 499 Val Ala Val Gln Pro Arg Gly Met Val Gly Ser Thr Pro Val Asn Cys 120 125 130 gac aac atc gca cca gga tac gat ttc ctc tcg ctg ctc acc cgc gaa 547 Asp Asn Ile Ala Pro Gly Tyr Asp Phe Leu Ser Leu Leu Thr Arg Glu 135 140 145 ggc gct ttc gtt aaa gaa tcc tgc gag atc ggc acc ccc ggc tac acc 595 Gly Ala Phe Val Lys Glu Ser Cys Glu Ile Gly Thr Pro Gly Tyr Thr 150 155 160 165 tcc agc ctg acc acc gac aac acc gcc aac gac tgg gag cgc gtc cgc 643 Ser Ser Leu Thr Thr Asp Asn Thr Ala Asn Asp Trp Glu Arg Val Arg 170 175 180 caa gca ctt ggc gat gac aag atc tcc atc ttc gga ctg tcc tac gga 691 Gln Ala Leu Gly Asp Asp Lys Ile Ser Ile Phe Gly Leu Ser Tyr Gly 185 190 195 acc tac ctc gga tcg gtc tac gcc acc cgc tac cca cag cac acc gac 739 Thr Tyr Leu Gly Ser Val Tyr Ala Thr Arg Tyr Pro Gln His Thr Asp 200 205 210 aag gtt gtc ctc gat tcc gca atg gcg ccc agc ctg gca tgg aac ggc 787 Lys Val Val Leu Asp Ser Ala Met Ala Pro Ser Leu Ala Trp Asn Gly 215 220 225 atc atg gcc tcc caa gaa cag ggc tac aaa aac tcc ctc aac gac ttc 835 Ile Met Ala Ser Gln Glu Gln Gly Tyr Lys Asn Ser Leu Asn Asp Phe 230 235 240 245 ttc acc tgg gtt gca gaa aac aac gac acg tat ggc ctc ggc act acc 883 Phe Thr Trp Val Ala Glu Asn Asn Asp Thr Tyr Gly Leu Gly Thr Thr 250 255 260 cca cta gcc gtg tac caa aac tgg tca aac aag atc gtc gcc gaa acc 931 Pro Leu Ala Val Tyr Gln Asn Trp Ser Asn Lys Ile Val Ala Glu Thr 265 270 275 gga acc aac cca acc gtt gct cca cca cca gca caa gtt ggc gat gtc 979 Gly Thr Asn Pro Thr Val Ala Pro Pro Ala Gln Val Gly Asp Val 280 285 290 cca cca gca ttc gca tgg gcc ggc caa gca ggc gca gac atg atg acc 1027 Pro Pro Ala Phe Ala Trp Ala Gly Gln Ala Gly Ala Asp Met Met Thr 295 300 305 gcc acc aac cca acc tcc gtg caa ctc cag ggc ctt gcc acc cag ctc 1075 Ala Thr Asn Pro Thr Ser Val Gln Leu Gln Gly Leu Ala Thr Gln Leu 310 315 320 325 cta aac cct gga tcc aac cag tca ctg agc cct ctg ctc aac gtc acc 1123 Leu Asn Pro Gly Ser Asn Gln Ser Leu Ser Pro Leu Leu Asn Val Thr 330 335 340 cgc gcc tac atc cca cag cca tca acc tgg ccc atg ctc gca ggc gcc 1171 Arg Ala Tyr Ile Pro Gln Pro Ser Thr Trp Pro Met Leu Ala Gly Ala 345 350 355 atc tca ggg caa aca ccc atc cct gac gta act gac acc ggc gac gac 1219 Ile Ser Gly Gln Thr Pro Ile Pro Asp Val Thr Asp Thr Gly Asp Asp 360 365 370 cca tac gtc atc gaa agc atc aac gcc agc gtc aac atg cag cgc atg 1267 Pro Tyr Val Ile Glu Ser Ile Asn Ala Ser Val Asn Met Gln Arg Met 375 380 385 gtc atg tgc aac gaa aac acc gtc gca cca gac cca gta gca atg gca 1315 Val Met Cys Asn Glu Asn Thr Val Ala Pro Asp Pro Val Ala Met Ala 390 395 400 405 cgc atg gcc tgg aca agc atg gtc acc ggc gac gtc ttt gac att tac 1363 Arg Met Ala Trp Thr Ser Met Val Thr Gly Asp Val Phe Asp Ile Tyr 410 415 420 tcc gtt aaa ttc agc tcc gga caa gcc tgc tcc ggc atc acc cca aca 1411 Ser Val Lys Phe Ser Ser Gly Gln Ala Cys Ser Gly Ile Thr Pro Thr 425 430 435 agc ggc cgc cag cca acc gac gga tct caa cta gca gtc caa cca cta 1459 Ser Gly Arg Gln Pro Thr Asp Gly Ser Gln Leu Ala Val Gln Pro Leu 440 445 450 ctc ctc cag gga acc agc gac cca caa acc cca tac tgg acc cac aac 1507 Leu Leu Gln Gly Thr Ser Asp Pro Gln Thr Pro Tyr Trp Thr His Asn 455 460 465 gag ctt gcc gac gcc atg aac gcc cac gtg gtc acc gtc aac gga cca 1555 Glu Leu Ala Asp Ala Met Asn Ala His Val Val Thr Val Asn Gly Pro 470 475 480 485 gga cac ggc caa tcc atc ggc ggc acc aac caa gca atc aac gac att 1603 Gly His Gly Gln Ser Ile Gly Gly Thr Asn Gln Ala Ile Asn Asp Ile 490 495 500 gtt gtg gac tac ctc cgc acc gga cac acc gac gcc acc tgg gtc gaa 1651 Val Val Asp Tyr Leu Arg Thr Gly His Thr Asp Ala Thr Trp Val Glu 505 510 515 ggc aac aca ccc acc cca att acg gct ggc taattgcttt ccacttagta 1701 Gly Asn Thr Pro Thr Pro Ile Thr Ala Gly 520 525 gatggtgata 1711 <210> 44 <211> 527 <212> PRT <213> Corynebacterium glutamicum <400> 44 Met Pro Arg Ser Thr Ile Lys Arg Val Val Ala Val Leu Ala Ala Ser 1 5 10 15 Thr Ala Leu Ser Pro Phe Leu Val Ser Met Pro Thr Ala Ala Ala Gln 20 25 30 Glu Asn Ile Arg Trp Glu Glu Cys Pro Pro Gln Val Asp Ile Ala Ser 35 40 45 Ala Gln Cys Gly Ser Ile Asp Val Pro Met His Tyr Ser Asp Pro Ser 50 55 60 Leu Gly Asp Ile Ser Val Gly Phe Val Lys Val Pro Ala Gln Gly Glu 65 70 75 80 Lys His Gly Thr Ile Phe Gly Asn Ser Gly Gly Pro Gly Gly Asp Ala 85 90 95 Tyr Ser Phe Phe Gly Ser Gln Ser Met Asn Trp Pro Glu Ala Met Tyr 100 105 110 Gln Asn Tyr Asp Leu Val Ala Val Gln Pro Arg Gly Met Val Gly Ser 115 120 125 Thr Pro Val Asn Cys Asp Asn Ile Ala Pro Gly Tyr Asp Phe Leu Ser 130 135 140 Leu Leu Thr Arg Glu Gly Ala Phe Val Lys Glu Ser Cys Glu Ile Gly 145 150 155 160 Thr Pro Gly Tyr Thr Ser Ser Leu Thr Thr Asp Asn Thr Ala Asn Asp 165 170 175 Trp Glu Arg Val Arg Gln Ala Leu Gly Asp Asp Lys Ile Ser Ile Phe 180 185 190 Gly Leu Ser Tyr Gly Thr Tyr Leu Gly Ser Val Tyr Ala Thr Arg Tyr 195 200 205 Pro Gln His Thr Asp Lys Val Val Leu Asp Ser Ala Met Ala Pro Ser 210 215 220 Leu Ala Trp Asn Gly Ile Met Ala Ser Gln Glu Gln Gly Tyr Lys Asn 225 230 235 240 Ser Leu Asn Asp Phe Phe Thr Trp Val Ala Glu Asn Asn Asp Thr Tyr 245 250 255 Gly Leu Gly Thr Thr Pro Leu Ala Val Tyr Gln Asn Trp Ser Asn Lys 260 265 270 Ile Val Ala Glu Thr Gly Thr Asn Pro Thr Val Ala Pro Pro Pro Ala 275 280 285 Gln Val Gly Asp Val Pro Pro Ala Phe Ala Trp Ala Gly Gln Ala Gly 290 295 300 Ala Asp Met Met Thr Ala Thr Asn Pro Thr Ser Val Gln Leu Gln Gly 305 310 315 320 Leu Ala Thr Gln Leu Leu Asn Pro Gly Ser Asn Gln Ser Leu Ser Pro 325 330 335 Leu Leu Asn Val Thr Arg Ala Tyr Ile Pro Gln Pro Ser Thr Trp Pro 340 345 350 Met Leu Ala Gly Ala Ile Ser Gly Gln Thr Pro Ile Pro Asp Val Thr 355 360 365 Asp Thr Gly Asp Asp Pro Tyr Val Ile Glu Ser Ile Asn Ala Ser Val 370 375 380 Asn Met Gln Arg Met Val Met Cys Asn Glu Asn Thr Val Ala Pro Asp 385 390 395 400 Pro Val Ala Met Ala Arg Met Ala Trp Thr Ser Met Val Thr Gly Asp 405 410 415 Val Phe Asp Ile Tyr Ser Val Lys Phe Ser Ser Gly Gln Ala Cys Ser 420 425 430 Gly Ile Thr Pro Thr Ser Gly Arg Gln Pro Thr Asp Gly Ser Gln Leu 435 440 445 Ala Val Gln Pro Leu Leu Leu Gln Gly Thr Ser Asp Pro Gln Thr Pro 450 455 460 Tyr Trp Thr His Asn Glu Leu Ala Asp Ala Met Asn Ala His Val Val 465 470 475 480 Thr Val Asn Gly Pro Gly His Gly Gln Ser Ile Gly Gly Thr Asn Gln 485 490 495 Ala Ile Asn Asp Ile Val Val Asp Tyr Leu Arg Thr Gly His Thr Asp 500 505 510 Ala Thr Trp Val Glu Gly Asn Thr Pro Thr Pro Ile Thr Ala Gly 515 520 525 <210> 45 <211> 2731 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (2701) <223> RXA01014 <400> 45 tcttaaagtt ttctagcaat ccacactagg cgcgaactat cgtggtgtca ttgcgcacct 60 tctaagggta gcgccccctc aaatttcaag gagcattaaa ttg acg tcc act aat 115 Leu Thr Ser Thr Asn 1 5 ctc acc cga cag gaa gct tcg gat cgt tcg agg tta ctg agt gta gaa 163 Leu Thr Arg Gln Glu Ala Ser Asp Arg Ser Arg Leu Leu Ser Val Glu 10 15 20 aac tat gac att gca ctt gat ctc aac aac ggt gat gag ttt ttt agt 211 Asn Tyr Asp Ile Ala Leu Asp Leu Asn Asn Gly Asp Glu Phe Phe Ser 25 30 35 tcc tcc acc gtt gtc agc ttc act gtc agg aag gct ggc gat acc ttt 259 Ser Ser Thr Val Val Ser Phe Thr Val Arg Lys Ala Gly Asp Thr Phe 40 45 50 att gat ctc cgc gca gca agc gtt gag gag gtt cgc ctg gac aat gtg 307 Ile Asp Leu Arg Ala Ala Ser Val Glu Glu Val Arg Leu Asp Asn Val 55 60 65 tcc atc aaa gat gag gct cta acc ctt ggc aag aac ggc tac gac gag 355 Ser Ile Lys Asp Glu Ala Leu Thr Leu Gly Lys Asn Gly Tyr Asp Glu 70 75 80 85 acg ttc ggc atc gcc ctg aag ggt ctt act ccc ggc gcg cac acc ttg 403 Thr Phe Gly Ile Ala Leu Lys Gly Leu Thr Pro Gly Ala His Thr Leu 90 95 100 cgg gta acg gcg tct atc ccc tat tcc cgc acc ggt gaa ggc ctg cac 451 Arg Val Thr Ala Ser Ile Pro Tyr Ser Arg Thr Gly Glu Gly Leu His 105 110 115 cgc atg gtg gat cca gca gac aat gag gtg tat ttg tac acc cag ttt 499 Arg Met Val Asp Pro Ala Asp Asn Glu Val Tyr Leu Tyr Thr Gln Phe 120 125 130 gag acc gcc gat gcc aag cgt atg ttc gcg tgt ttc gat cag cca gac 547 Glu Thr Ala Asp Ala Lys Arg Met Phe Ala Cys Phe Asp Gln Pro Asp 135 140 145 ctc aag gct acc tat gat ctg aac atc aaa act cct aag ggt tgg aag 595 Leu Lys Ala Thr Tyr Asp Leu Asn Ile Lys Thr Pro Lys Gly Trp Lys 150 155 160 165 atc att tcc aac tct gag cag cag gtt tcc act cag cac act gat tac 643 Ile Ile Ser Asn Ser Glu Gln Gln Val Ser Thr Gln His Thr Asp Tyr 170 175 180 gat acc cac att tcc cga gtg gac tat ccc ctc tcc acc tac ctg att 691 Asp Thr His Ile Ser Arg Val Asp Tyr Pro Leu Ser Thr Tyr Leu Ile 185 190 195 gcg gtg tgc gcg ggt cgt tac cac gag gtg tgc gat gtc tgg aag ggt 739 Ala Val Cys Ala Gly Arg Tyr His Glu Val Cys Asp Val Trp Lys Gly 200 205 210 acg ctc acc cac cat gca gaa aca cct gcc gat cag cca act gag ctg 787 Thr Leu Thr His His Ala Glu Thr Pro Ala Asp Gln Pro Thr Glu Leu 215 220 225 act gtt ccg ctt gct ctc tac tgc cgc agt tct ttg gct aaa gat ctt 835 Thr Val Pro Leu Ala Leu Tyr Cys Arg Ser Ser Leu Ala Lys Asp Leu 230 235 240 245 gat gcg gtg cgt ctg ttt acc gaa acg aag cag ggc ttt gat tgg tac 883 Asp Ala Val Arg Leu Phe Thr Glu Thr Lys Gln Gly Phe Asp Trp Tyr 250 255 260 cac cgc aac ttc ggt gtg gcg tac cca ttc ggc aag tac gat cag atc 931 His Arg Asn Phe Gly Val Ala Tyr Pro Phe Gly Lys Tyr Asp Gln Ile 265 270 275 ttc gtc cct gaa ttt aat gct ggc gcg atg gag aac gcc ggc gct gtc 979 Phe Val Pro Glu Phe Asn Ala Gly Ala Met Glu Asn Ala Gly Ala Val 280 285 290 acc atc cgc gat gag tac gtt ttt gca tcc aag gca acc cgt tac cgc 1027 Thr Ile Arg Asp Glu Tyr Val Phe Ala Ser Lys Ala Thr Arg Tyr Arg 295 300 305 tac gag cgc cgc gct gaa acc atc ctt cac gag ctc gct cac atg tgg 1075 Tyr Glu Arg Arg Ala Glu Thr Ile Leu His Glu Leu Ala His Met Trp 310 315 320 325 ttc ggt gac ctg gtg acc atg cag tgg tgg gat gat ctg tgg ctc aac 1123 Phe Gly Asp Leu Val Thr Met Gln Trp Trp Asp Asp Leu Trp Leu Asn 330 335 340 gag tcc ttc gcc act tgg tcc gcg gca att tct cag gct gag gaa act 1171 Glu Ser Phe Ala Thr Trp Ser Ala Ala Ile Ser Gln Ala Glu Glu Thr 345 350 355 gaa tac aac act gca tgg gtg act ttc gcc aat gtg gag aag tcg tgg 1219 Glu Tyr Asn Thr Ala Trp Val Thr Phe Ala Asn Val Glu Lys Ser Trp 360 365 370 gcg tac cag cag gat cag ctg cct tcc acc cac ccg gtg ttc tct gac 1267 Ala Tyr Gln Gln Asp Gln Leu Pro Ser Thr His Pro Val Phe Ser Asp 375 380 385 gga tac gac att gag act gtc gac cag aac ttc gac ggc atc acc tac 1315 Gly Tyr Asp Ile Glu Thr Val Asp Gln Asn Phe Asp Gly Ile Thr Tyr 390 395 400 405 gca aag ggc gcc tcg gtg ctc aag cag ctg cag gca tac gtt ggc cgt 1363 Ala Lys Gly Ala Ser Val Leu Lys Gln Leu Gln Ala Tyr Val Gly Arg 410 415 420 gag gaa ttc ctg gca ggc gta cgc agg cac ttt gcc aac cac gca tgg 1411 Glu Glu Phe Leu Ala Gly Val Arg Arg His Phe Ala Asn His Ala Trp 425 430 435 ggc aac gcc agc ttt gat gat ctg ctc ggc gcc ctc gag cag tcc tcc 1459 Gly Asn Ala Ser Phe Asp Asp Leu Leu Gly Ala Leu Glu Gln Ser Ser 440 445 450 ggc cgc gac ctc tcc gac tgg gca aac cag tgg ctc aag acc acc ggc 1507 Gly Arg Asp Leu Ser Asp Trp Ala Asn Gln Trp Leu Lys Thr Thr Gly 455 460 465 atc aac acc ctc ggc gca aag ttc acc acc gac aac ggc aaa tac acc 1555 Ile Asn Thr Leu Gly Ala Lys Phe Thr Thr Asp Asn Gly Lys Tyr Thr 470 475 480 485 tcc ttc tcc gtc acc cag acc ggc gcc gcg ccg ggt gcc ggt gag ctg 1603 Ser Phe Ser Val Thr Gln Thr Gly Ala Ala Pro Gly Ala Gly Glu Leu 490 495 500 cgg act cac cgc atc gcg gtg ggt ctt tat aag ctt gtc gac gga tcc 1651 Arg Thr His Arg Ile Ala Val Gly Leu Tyr Lys Leu Val Asp Gly Ser 505 510 515 ctc aac cgc tac gca cga gta gaa ctt gac tgc agt ggc gcg tcg aca 1699 Leu Asn Arg Tyr Ala Arg Val Glu Leu Asp Cys Ser Gly Ala Ser Thr 520 525 530 agc gtt gaa gag atc gtt gga ctt gag cag gct gac ttc gtg ctg gtc 1747 Ser Val Glu Glu Ile Val Gly Leu Glu Gln Ala Asp Phe Val Leu Val 535 540 545 aac gat gat gat ctg acg tat gcg ctg ctg gat ctg gat gat gat tca 1795 Asn Asp Asp Asp Leu Thr Tyr Ala Leu Leu Asp Leu Asp Asp Asp Ser 550 555 560 565 cgc aat ttt gtc atc gac aat att gat aag ttc agc gac cct atg cct 1843 Arg Asn Phe Val Ile Asp Asn Ile Asp Lys Phe Ser Asp Pro Met Pro 570 575 580 cgc acg ctg gtg tgg tcc gct gcg tgg gag atg act cgc gct ggt cag 1891 Arg Thr Leu Val Trp Ser Ala Ala Trp Glu Met Thr Arg Ala Gly Gln 585 590 595 atg aag gct cgt gat ttc atc gcg ctg gtt gct cgt ggc gct gct gcg 1939 Met Lys Ala Arg Asp Phe Ile Ala Leu Val Ala Arg Gly Ala Ala Ala 600 605 610 gaa act gaa att gct gtg ctg gag cgc att ctc gcg cag gct acc tct 1987 Glu Thr Glu Ile Ala Val Leu Glu Arg Ile Leu Ala Gln Ala Thr Ser 615 620 625 gcg ctg aag agc tac gcc gac cca gcg tgg gca gaa gca act gga aat 2035 Ala Leu Lys Ser Tyr Ala Asp Pro Ala Trp Ala Glu Ala Thr Gly Asn 630 635 640 645 gac ctg ctg gcc gat gct ttc ctt gag ggt gct cgc tcc gca gaa cca 2083 Asp Leu Leu Ala Asp Ala Phe Leu Glu Gly Ala Arg Ser Ala Glu Pro 650 655 660 gac tcc gac act cag ttg gcg ttc att cag gct ctg gca aaa gca acg 2131 Asp Ser Asp Thr Gln Leu Ala Phe Ile Gln Ala Leu Ala Lys Ala Thr 665 670 675 ctc aat gat gct gct gcc gat tac ttc cgc gac att ctt gcc ggc aac 2179 Leu Asn Asp Ala Ala Ala Asp Tyr Phe Arg Asp Ile Leu Ala Gly Asn 680 685 690 gtc gaa ggc ctg acc gtg gat cct gac ctg cgt tgg tgg gca ctg act 2227 Val Glu Gly Leu Thr Val Asp Pro Asp Leu Arg Trp Trp Ala Leu Thr 695 700 705 gcg ctt atc gcc cgt ggt gac atc gag gct gtc gaa gat gca atc gcc 2275 Ala Leu Ile Ala Arg Gly Asp Ile Glu Ala Val Glu Asp Ala Ile Ala 710 715 720 725 gct gaa ctt tcc cgc gac aac tcc agt gcc tcc ttc ctc gca tca ctt 2323 Ala Glu Leu Ser Arg Asp Asn Ser Ser Ala Ser Phe Leu Ala Ser Leu 730 735 740 cga gcc ggt gcc gct gtg aac act gaa gaa gtg aag gct gct gca tac 2371 Arg Ala Gly Ala Ala Val Asn Thr Glu Glu Val Lys Ala Ala Ala Tyr 745 750 755 aag cat gtc acg gca gtt gat agt ggc cta tcc aac ctg gag ctg cgc 2419 Lys His Val Thr Ala Val Asp Ser Gly Leu Ser Asn Leu Glu Leu Arg 760 765 770 cac aag att gaa ggc ctc aca ttc act ggc tct tct gaa ctg ctg caa 2467 His Lys Ile Glu Gly Leu Thr Phe Thr Gly Ser Ser Glu Leu Leu Gln 775 780 785 gcc tac aac gag cag tac ttc gaa atc ctt gat gat gtg tgg gcg aac 2515 Ala Tyr Asn Glu Gln Tyr Phe Glu Ile Leu Asp Asp Val Trp Ala Asn 790 795 800 805 ttc tcc ggc gaa atg gca cag cag atc gtc ctc gga ctg ttc cct tca 2563 Phe Ser Gly Glu Met Ala Gln Gln Ile Val Leu Gly Leu Phe Pro Ser 810 815 820 tgg aac gtt tcc gaa gag ggt ctc aag cgt acc gac gag ttt ctt gat 2611 Trp Asn Val Ser Glu Glu Gly Leu Lys Arg Thr Asp Glu Phe Leu Asp 825 830 835 ggc gaa cat gtc gca ggc atc aag cga att gtt tcc gaa tcc ctc gac 2659 Gly Glu His Val Ala Gly Ile Lys Arg Ile Val Ser Glu Ser Leu Asp 840 845 850 cgc act gcc cgt gct ctg cgc aac cgt gcg gca gat gct gcg 2701 Arg Thr Ala Arg Ala Leu Arg Asn Arg Ala Ala Asp Ala Ala 855 860 865 taagtaaaag attctcaatc ccaaaaaatc 2731 <210> 46 <211> 867 <212> PRT <213> Corynebacterium glutamicum <400> 46 Leu Thr Ser Thr Asn Leu Thr Arg Gln Glu Ala Ser Asp Arg Ser Arg 1 5 10 15 Leu Leu Ser Val Glu Asn Tyr Asp Ile Ala Leu Asp Leu Asn Asn Gly 20 25 30 Asp Glu Phe Phe Ser Ser Ser Thr Val Val Ser Phe Thr Val Arg Lys 35 40 45 Ala Gly Asp Thr Phe Ile Asp Leu Arg Ala Ala Ser Val Glu Glu Val 50 55 60 Arg Leu Asp Asn Val Ser Ile Lys Asp Glu Ala Leu Thr Leu Gly Lys 65 70 75 80 Asn Gly Tyr Asp Glu Thr Phe Gly Ile Ala Leu Lys Gly Leu Thr Pro 85 90 95 Gly Ala His Thr Leu Arg Val Thr Ala Ser Ile Pro Tyr Ser Arg Thr 100 105 110 Gly Glu Gly Leu His Arg Met Val Asp Pro Ala Asp Asn Glu Val Tyr 115 120 125 Leu Tyr Thr Gln Phe Glu Thr Ala Asp Ala Lys Arg Met Phe Ala Cys 130 135 140 Phe Asp Gln Pro Asp Leu Lys Ala Thr Tyr Asp Leu Asn Ile Lys Thr 145 150 155 160 Pro Lys Gly Trp Lys Ile Ile Ser Asn Ser Glu Gln Gln Val Ser Thr 165 170 175 Gln His Thr Asp Tyr Asp Thr His Ile Ser Arg Val Asp Tyr Pro Leu 180 185 190 Ser Thr Tyr Leu Ile Ala Val Cys Ala Gly Arg Tyr His Glu Val Cys 195 200 205 Asp Val Trp Lys Gly Thr Leu Thr His His Ala Glu Thr Pro Ala Asp 210 215 220 Gln Pro Thr Glu Leu Thr Val Pro Leu Ala Leu Tyr Cys Arg Ser Ser 225 230 235 240 Leu Ala Lys Asp Leu Asp Ala Val Arg Leu Phe Thr Glu Thr Lys Gln 245 250 255 Gly Phe Asp Trp Tyr His Arg Asn Phe Gly Val Ala Tyr Pro Phe Gly 260 265 270 Lys Tyr Asp Gln Ile Phe Val Pro Glu Phe Asn Ala Gly Ala Met Glu 275 280 285 Asn Ala Gly Ala Val Thr Ile Arg Asp Glu Tyr Val Phe Ala Ser Lys 290 295 300 Ala Thr Arg Tyr Arg Tyr Glu Arg Arg Ala Glu Thr Ile Leu His Glu 305 310 315 320 Leu Ala His Met Trp Phe Gly Asp Leu Val Thr Met Gln Trp Trp Asp 325 330 335 Asp Leu Trp Leu Asn Glu Ser Phe Ala Thr Trp Ser Ala Ala Ile Ser 340 345 350 Gln Ala Glu Glu Thr Glu Tyr Asn Thr Ala Trp Val Thr Phe Ala Asn 355 360 365 Val Glu Lys Ser Trp Ala Tyr Gln Gln Asp Gln Leu Pro Ser Thr His 370 375 380 Pro Val Phe Ser Asp Gly Tyr Asp Ile Glu Thr Val Asp Gln Asn Phe 385 390 395 400 Asp Gly Ile Thr Tyr Ala Lys Gly Ala Ser Val Leu Lys Gln Leu Gln 405 410 415 Ala Tyr Val Gly Arg Glu Glu Phe Leu Ala Gly Val Arg Arg His Phe 420 425 430 Ala Asn His Ala Trp Gly Asn Ala Ser Phe Asp Asp Leu Leu Gly Ala 435 440 445 Leu Glu Gln Ser Ser Gly Arg Asp Leu Ser Asp Trp Ala Asn Gln Trp 450 455 460 Leu Lys Thr Thr Gly Ile Asn Thr Leu Gly Ala Lys Phe Thr Thr Asp 465 470 475 480 Asn Gly Lys Tyr Thr Ser Phe Ser Val Thr Gln Thr Gly Ala Ala Pro 485 490 495 Gly Ala Gly Glu Leu Arg Thr His Arg Ile Ala Val Gly Leu Tyr Lys 500 505 510 Leu Val Asp Gly Ser Leu Asn Arg Tyr Ala Arg Val Glu Leu Asp Cys 515 520 525 Ser Gly Ala Ser Thr Ser Val Glu Glu Ile Val Gly Leu Glu Gln Ala 530 535 540 Asp Phe Val Leu Val Asn Asp Asp Asp Leu Thr Tyr Ala Leu Leu Asp 545 550 555 560 Leu Asp Asp Asp Ser Arg Asn Phe Val Ile Asp Asn Ile Asp Lys Phe 565 570 575 Ser Asp Pro Met Pro Arg Thr Leu Val Trp Ser Ala Ala Trp Glu Met 580 585 590 Thr Arg Ala Gly Gln Met Lys Ala Arg Asp Phe Ile Ala Leu Val Ala 595 600 605 Arg Gly Ala Ala Ala Glu Thr Glu Ile Ala Val Leu Glu Arg Ile Leu 610 615 620 Ala Gln Ala Thr Ser Ala Leu Lys Ser Tyr Ala Asp Pro Ala Trp Ala 625 630 635 640 Glu Ala Thr Gly Asn Asp Leu Leu Ala Asp Ala Phe Leu Glu Gly Ala 645 650 655 Arg Ser Ala Glu Pro Asp Ser Asp Thr Gln Leu Ala Phe Ile Gln Ala 660 665 670 Leu Ala Lys Ala Thr Leu Asn Asp Ala Ala Ala Asp Tyr Phe Arg Asp 675 680 685 Ile Leu Ala Gly Asn Val Glu Gly Leu Thr Val Asp Pro Asp Leu Arg 690 695 700 Trp Trp Ala Leu Thr Ala Leu Ile Ala Arg Gly Asp Ile Glu Ala Val 705 710 715 720 Glu Asp Ala Ile Ala Ala Glu Leu Ser Arg Asp Asn Ser Ser Ala Ser 725 730 735 Phe Leu Ala Ser Leu Arg Ala Gly Ala Ala Val Asn Thr Glu Glu Val 740 745 750 Lys Ala Ala Ala Tyr Lys His Val Thr Ala Val Asp Ser Gly Leu Ser 755 760 765 Asn Leu Glu Leu Arg His Lys Ile Glu Gly Leu Thr Phe Thr Gly Ser 770 775 780 Ser Glu Leu Leu Gln Ala Tyr Asn Glu Gln Tyr Phe Glu Ile Leu Asp 785 790 795 800 Asp Val Trp Ala Asn Phe Ser Gly Glu Met Ala Gln Gln Ile Val Leu 805 810 815 Gly Leu Phe Pro Ser Trp Asn Val Ser Glu Glu Gly Leu Lys Arg Thr 820 825 830 Asp Glu Phe Leu Asp Gly Glu His Val Ala Gly Ile Lys Arg Ile Val 835 840 845 Ser Glu Ser Leu Asp Arg Thr Ala Arg Ala Leu Arg Asn Arg Ala Ala 850 855 860 Asp Ala Ala 865 <210> 47 <211> 2038 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (2008) <223> RXA01046 <400> 47 actcggcgta cacggaaaat agcagctgat ccatctaggg tcagtgaact ctcgcggatt 60 gccacacacc accaaccact acaagaaaga accccctacc gtg cct ttt cac gat 115 Val Pro Phe His Asp 1 5 ttc act gac cct gat agc gat cct agg caa aat gca cca gct aac ccc 163 Phe Thr Asp Pro Asp Ser Asp Pro Arg Gln Asn Ala Pro Ala Asn Pro 10 15 20 acc gct caa ccg cca tct gcc ccc acc tca cac tca tca cag cag ggt 211 Thr Ala Gln Pro Pro Ser Ala Pro Thr Ser His Ser Ser Gln Gln Gly 25 30 35 ctc cct ccg ggc gcc att atg atc cct ttt cca ggg caa ccg cag cag 259 Leu Pro Pro Gly Ala Ile Met Ile Pro Phe Pro Gly Gln Pro Gln Gln 40 45 50 caa agc gca ccc aat gac gag acc cgt ttc atc gac ctt aac gaa cgt 307 Gln Ser Ala Pro Asn Asp Glu Thr Arg Phe Ile Asp Leu Asn Glu Arg 55 60 65 cat aaa gat gat gaa cca gcc ctg ttt cgc gat gat gtt att gat caa 355 His Lys Asp Asp Glu Pro Ala Leu Phe Arg Asp Asp Val Ile Asp Gln 70 75 80 85 act ctc gct att ttg atc agt aaa aat aag ccc aat gcg cta ctc gtt 403 Thr Leu Ala Ile Leu Ile Ser Lys Asn Lys Pro Asn Ala Leu Leu Val 90 95 100 ggg cct gcc ggt aca ggt aaa tcc cgt atc gca gaa gat att gcg cgc 451 Gly Pro Ala Gly Thr Gly Lys Ser Arg Ile Ala Glu Asp Ile Ala Arg 105 110 115 cgc ctt gcc aat gat gac gta tct att ccc gat cag ctt gtc ggc cac 499 Arg Leu Ala Asn Asp Asp Val Ser Ile Pro Asp Gln Leu Val Gly His 120 125 130 cgt att ctt gat gtc tcc att gca gag ctt gtt gct ggt gct ggc gtt 547 Arg Ile Leu Asp Val Ser Ile Ala Glu Leu Val Ala Gly Ala Gly Val 135 140 145 gtt ggt cag ctc gaa gaa cgc att ctg gat ctc atc aag tat gcg acc 595 Val Gly Gln Leu Glu Glu Arg Ile Leu Asp Leu Ile Lys Tyr Ala Thr 150 155 160 165 gac ccg agt aac aaa gtc att atc ttt att gac gag att cac caa att 643 Asp Pro Ser Asn Lys Val Ile Ile Phe Ile Asp Glu Ile His Gln Ile 170 175 180 gct ggt gat cag tcc agt cac agt gga tcg caa gcc aaa gtt gct cag 691 Ala Gly Asp Gln Ser Ser His Ser Gly Ser Gln Ala Lys Val Ala Gln 185 190 195 att ctc aaa ccc tat ctt gcc cgt ggt gac ctt cgt gtt att ggt gcc 739 Ile Leu Lys Pro Tyr Leu Ala Arg Gly Asp Leu Arg Val Ile Gly Ala 200 205 210 acc acc acc cag gaa gct cgt gac ttc gat cat gat cca gcc ctc aaa 787 Thr Thr Thr Gln Glu Ala Arg Asp Phe Asp His Asp Pro Ala Leu Lys 215 220 225 cgc cgt ttt agc aga gta aat gtc gat gaa ttt gat cga gat caa acg 835 Arg Arg Phe Ser Arg Val Asn Val Asp Glu Phe Asp Arg Asp Gln Thr 230 235 240 245 ctc act att ctt cat gct gca cgt gat ggt tac ctc aaa cat ttc aac 883 Leu Thr Ile Leu His Ala Ala Arg Asp Gly Tyr Leu Lys His Phe Asn 250 255 260 aac gct gtc acg gta tct gac gac gta ctg ggc tat gtc tac acc tac 931 Asn Ala Val Thr Val Ser Asp Asp Val Leu Gly Tyr Val Tyr Thr Tyr 265 270 275 tcg cag caa ttc aac cca ggc aat aca gca caa cct gat gca gca ctg 979 Ser Gln Gln Phe Asn Pro Gly Asn Thr Ala Gln Pro Asp Ala Ala Leu 280 285 290 acg ctg ttt gat aag gcg ttg gct tcc cta act atg gag aaa cag cgt 1027 Thr Leu Phe Asp Lys Ala Leu Ala Ser Leu Thr Met Glu Lys Gln Arg 295 300 305 ctg atc aac aac cat gtc att gcg ccg tcg ctc aag ttc cct gtg tca 1075 Leu Ile Asn Asn His Val Ile Ala Pro Ser Leu Lys Phe Pro Val Ser 310 315 320 325 gaa agg cac atc cat aac acc gct cgc aaa ctt gcc ttt ggc tct caa 1123 Glu Arg His Ile His Asn Thr Ala Arg Lys Leu Ala Phe Gly Ser Gln 330 335 340 gtg cca gcc tcc atc aat act gat gat gct cgt gac aaa ctc gaa acg 1171 Val Pro Ala Ser Ile Asn Thr Asp Asp Ala Arg Asp Lys Leu Glu Thr 345 350 355 ttg ttt ggt caa gat cat att att gag cca gta ctc acc gct atc aag 1219 Leu Phe Gly Gln Asp His Ile Ile Glu Pro Val Leu Thr Ala Ile Lys 360 365 370 cgt gaa cag ctt ggt att ttc cct cgc acc aaa cca ttg agc tgg gtg 1267 Arg Glu Gln Leu Gly Ile Phe Pro Arg Thr Lys Pro Leu Ser Trp Val 375 380 385 ttt gct ggt tca tct ggt gtg ggt aaa aca gaa atg gcg cgt att ctc 1315 Phe Ala Gly Ser Ser Gly Val Gly Lys Thr Glu Met Ala Arg Ile Leu 390 395 400 405 tct cgc gcc att aat ggc ggc gat ccc atc att atc aat ggt ccc gaa 1363 Ser Arg Ala Ile Asn Gly Gly Asp Pro Ile Ile Ile Ile Asn Gly Pro Glu 410 415 420 tac att agt cct gag tcc att act ggc ctt atc gga tca tcc gat ggc 1411 Tyr Ile Ser Pro Glu Ser Ile Thr Gly Leu Ile Gly Ser Ser Asp Gly 425 430 435 tat atc ggc tct aat tct aag cgt gct aaa cca ctc gac ccg ctg att 1459 Tyr Ile Gly Ser Asn Ser Lys Arg Ala Lys Pro Leu Asp Pro Leu Ile 440 445 450 tct aat ccg cgt cag gtg att gtg ctc gat gaa ttt gag aag tct cac 1507 Ser Asn Pro Arg Gln Val Ile Val Leu Asp Glu Phe Glu Lys Ser His 455 460 465 cct cat ttc cag caa ttg ttc atg gca gct ctt gat aca ggc act atg 1555 Pro His Phe Gln Gln Leu Phe Met Ala Ala Leu Asp Thr Gly Thr Met 470 475 480 485 gcg atg gct aat ggc acg aca ttg aat ttc tct cag gcc att atc att 1603 Ala Met Ala Asn Gly Thr Thr Leu Asn Phe Ser Gln Ala Ile Ile Ile 490 495 500 gcc acc acc aat gca gcc cgc gac aaa atc ggt cgt gac agc ttt gga 1651 Ala Thr Thr Asn Ala Ala Arg Asp Lys Ile Gly Arg Asp Ser Phe Gly 505 510 515 ttc gat tca gat aat tca ggt gtc ctc ggt tct gct caa gca gca act 1699 Phe Asp Ser Asp Asn Ser Gly Val Leu Gly Ser Ala Gln Ala Ala Thr 520 525 530 gat ccg cgt gca cag gaa cgc ctc aag tca ctg atg tcc aag gat ttc 1747 Asp Pro Arg Ala Gln Glu Arg Leu Lys Ser Leu Met Ser Lys Asp Phe 535 540 545 cct gtt gaa ctg ctc aac cgt ttc cag aat atc ttt gcc ttc aac cgc 1795 Pro Val Glu Leu Leu Asn Arg Phe Gln Asn Ile Phe Ala Phe Asn Arg 550 555 560 565 att gat gca ggc acc tac cgt gag att ctg gac aat ctc tac cag cgt 1843 Ile Asp Ala Gly Thr Tyr Arg Glu Ile Leu Asp Asn Leu Tyr Gln Arg 570 575 580 cgc cgt gac gcc gtg ctg ctt agc cac ccg cat tac gca gca cag atc 1891 Arg Arg Asp Ala Val Leu Leu Ser His Pro His Tyr Ala Ala Gln Ile 585 590 595 cct gca gat att gat tca gac act ctt gat cag ctg gtg gaa acc acc 1939 Pro Ala Asp Ile Asp Ser Asp Thr Leu Asp Gln Leu Val Glu Thr Thr 600 605 610 ttt atc tca gat ttt ggt gca cgt cct gct gca cgc acc atc gaa gac 1987 Phe Ile Ser Asp Phe Gly Ala Arg Pro Ala Ala Arg Thr Ile Glu Asp 615 620 625 cac atc gca tcc ttg ctg atg tgaccaacct tttaggagta catcatgatt 2038 His Ile Ala Ser Leu Leu Met 630 635 <210> 48 <211> 636 <212> PRT <213> Corynebacterium glutamicum <400> 48 Val Pro Phe His Asp Phe Thr Asp Pro Asp Ser Asp Pro Arg Gln Asn 1 5 10 15 Ala Pro Ala Asn Pro Thr Ala Gln Pro Pro Ser Ala Pro Thr Ser His 20 25 30 Ser Ser Gln Gln Gly Leu Pro Pro Gly Ala Ile Met Ile Pro Phe Pro 35 40 45 Gly Gln Pro Gln Gln Gln Ser Ala Pro Asn Asp Glu Thr Arg Phe Ile 50 55 60 Asp Leu Asn Glu Arg His Lys Asp Asp Glu Pro Ala Leu Phe Arg Asp 65 70 75 80 Asp Val Ile Asp Gln Thr Leu Ala Ile Leu Ile Ser Lys Asn Lys Pro 85 90 95 Asn Ala Leu Leu Val Gly Pro Ala Gly Thr Gly Lys Ser Arg Ile Ala 100 105 110 Glu Asp Ile Ala Arg Arg Leu Ala Asn Asp Asp Val Ser Ile Pro Asp 115 120 125 Gln Leu Val Gly His Arg Ile Leu Asp Val Ser Ile Ala Glu Leu Val 130 135 140 Ala Gly Ala Gly Val Val Gly Gln Leu Glu Glu Arg Ile Leu Asp Leu 145 150 155 160 Ile Lys Tyr Ala Thr Asp Pro Ser Asn Lys Val Ile Ile Phe Ile Asp 165 170 175 Glu Ile His Gln Ile Ala Gly Asp Gln Ser Ser His Ser Gly Ser Gln 180 185 190 Ala Lys Val Ala Gln Ile Leu Lys Pro Tyr Leu Ala Arg Gly Asp Leu 195 200 205 Arg Val Ile Gly Ala Thr Thr Thr Gln Glu Ala Arg Asp Phe Asp His 210 215 220 Asp Pro Ala Leu Lys Arg Arg Phe Ser Arg Val Asn Val Asp Glu Phe 225 230 235 240 Asp Arg Asp Gln Thr Leu Thr Ile Leu His Ala Ala Arg Asp Gly Tyr 245 250 255 Leu Lys His Phe Asn Asn Ala Val Thr Val Ser Asp Asp Val Leu Gly 260 265 270 Tyr Val Tyr Thr Tyr Ser Gln Gln Phe Asn Pro Gly Asn Thr Ala Gln 275 280 285 Pro Asp Ala Ala Leu Thr Leu Phe Asp Lys Ala Leu Ala Ser Leu Thr 290 295 300 Met Glu Lys Gln Arg Leu Ile Asn Asn His Val Ile Ala Pro Ser Leu 305 310 315 320 Lys Phe Pro Val Ser Glu Arg His Ile His Asn Thr Ala Arg Lys Leu 325 330 335 Ala Phe Gly Ser Gln Val Pro Ala Ser Ile Asn Thr Asp Asp Ala Arg 340 345 350 Asp Lys Leu Glu Thr Leu Phe Gly Gln Asp His Ile Ile Glu Pro Val 355 360 365 Leu Thr Ala Ile Lys Arg Glu Gln Leu Gly Ile Phe Pro Arg Thr Lys 370 375 380 Pro Leu Ser Trp Val Phe Ala Gly Ser Ser Gly Val Gly Lys Thr Glu 385 390 395 400 Met Ala Arg Ile Leu Ser Arg Ala Ile Asn Gly Gly Asp Pro Ile Ile 405 410 415 Ile Asn Gly Pro Glu Tyr Ile Ser Pro Glu Ser Ile Thr Gly Leu Ile 420 425 430 Gly Ser Ser Asp Gly Tyr Ile Gly Ser Asn Ser Lys Arg Ala Lys Pro 435 440 445 Leu Asp Pro Leu Ile Ser Asn Pro Arg Gln Val Ile Val Leu Asp Glu 450 455 460 Phe Glu Lys Ser His Pro His Phe Gln Gln Leu Phe Met Ala Ala Leu 465 470 475 480 Asp Thr Gly Thr Met Ala Met Ala Asn Gly Thr Thr Leu Asn Phe Ser 485 490 495 Gln Ala Ile Ile Ile Ala Thr Thr Asn Ala Ala Arg Asp Lys Ile Gly 500 505 510 Arg Asp Ser Phe Gly Phe Asp Ser Asp Asn Ser Gly Val Leu Gly Ser 515 520 525 Ala Gln Ala Ala Thr Asp Pro Arg Ala Gln Glu Arg Leu Lys Ser Leu 530 535 540 Met Ser Lys Asp Phe Pro Val Glu Leu Leu Asn Arg Phe Gln Asn Ile 545 550 555 560 Phe Ala Phe Asn Arg Ile Asp Ala Gly Thr Tyr Arg Glu Ile Leu Asp 565 570 575 Asn Leu Tyr Gln Arg Arg Arg Asp Ala Val Leu Leu Ser His Pro His 580 585 590 Tyr Ala Ala Gln Ile Pro Ala Asp Ile Asp Ser Asp Thr Leu Asp Gln 595 600 605 Leu Val Glu Thr Thr Phe Ile Ser Asp Phe Gly Ala Arg Pro Ala Ala 610 615 620 Arg Thr Ile Glu Asp His Ile Ala Ser Leu Leu Met 625 630 635 <210> 49 <211> 1456 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1426) <223> RXA01120 <400> 49 gaataaattc gggattcatt agcttaagcg taccgctctg cagagttcac aggtaaagcg 60 ctaagatgga acaacccatt gccaatattg ttggttagag ttg tac gca gta aat 115 Leu Tyr Ala Val Asn 1 5 ctt ttc aat cgt gga agc ggg tct cac agt cta atg gca cgt atg cag 163 Leu Phe Asn Arg Gly Ser Gly Ser His Ser Leu Met Ala Arg Met Gln 10 15 20 gaa agc gcc gat ctg ctc aaa tgt tcc ttc tgc gga aag agc caa aag 211 Glu Ser Ala Asp Leu Leu Lys Cys Ser Phe Cys Gly Lys Ser Gln Lys 25 30 35 cag gta aaa aaa ctc atc gcg ggt ggc gcc gta tat atc tgt gat gag 259 Gln Val Lys Lys Leu Ile Ala Gly Gly Ala Val Tyr Ile Cys Asp Glu 40 45 50 tgc att gag ctg tgc aac gag att att gaa gaa gaa ctc ggt caa gct 307 Cys Ile Glu Leu Cys Asn Glu Ile Ile Glu Glu Glu Leu Gly Gln Ala 55 60 65 caa cac gac gag cag gag cgc aac gag ctc ccc aag ccg tcg gag att 355 Gln His Asp Glu Gln Glu Arg Asn Glu Leu Pro Lys Pro Ser Glu Ile 70 75 80 85 tca gcc ttc ctt gat act tat gtc atc ggg cag gac cca gca aaa cgt 403 Ser Ala Phe Leu Asp Thr Tyr Val Ile Gly Gln Asp Pro Ala Lys Arg 90 95 100 atc ctg tcg gtt gcg gtg tac aac cat tac aag cgt ctc cgc gca tcg 451 Ile Leu Ser Val Ala Val Tyr Asn His Tyr Lys Arg Leu Arg Ala Ser 105 110 115 gaa acc atc ggt cgt cgc agg aat gac gag cct gaa acc gaa ctg gtt 499 Glu Thr Ile Gly Arg Arg Arg Asn Asp Glu Pro Glu Thr Glu Leu Val 120 125 130 aag tcc aat att ttg atg ctc ggc ccc act ggc tcc ggc aag act ttc 547 Lys Ser Asn Ile Leu Met Leu Gly Pro Thr Gly Ser Gly Lys Thr Phe 135 140 145 ctt gcc cag act ttg gca aag ctg ctg gat gtt cct ttt gct atc gcg 595 Leu Ala Gln Thr Leu Ala Lys Leu Leu Asp Val Pro Phe Ala Ile Ala 150 155 160 165 gat gcc acc tca ctg acc gag gct ggt tat gtg ggc gag gat gtg gaa 643 Asp Ala Thr Ser Leu Thr Glu Ala Gly Tyr Val Gly Glu Asp Val Glu 170 175 180 aac atc ttg ctc aag ctg ctt cag gct gct gat ttt gat gtg gaa cgt 691 Asn Ile Leu Leu Lys Leu Leu Gln Ala Ala Asp Phe Asp Val Glu Arg 185 190 195 gca cag cgc ggc atc att tac atc gat gaa gtg gac aag att tcc cgc 739 Ala Gln Arg Gly Ile Ile Tyr Ile Asp Glu Val Asp Lys Ile Ser Arg 200 205 210 aag tct gaa aac cca tcg atc act cgc gat gtt tcc ggt gaa ggc gtg 787 Lys Ser Glu Asn Pro Ser Ile Thr Arg Asp Val Ser Gly Glu Gly Val 215 220 225 cag cag gca ctg ctg aaa att ttg gaa ggc act gtc gcc gca atc cca 835 Gln Gln Ala Leu Leu Lys Ile Leu Glu Gly Thr Val Ala Ala Ile Pro 230 235 240 245 ccg cag gga gga cgc aag cac ccc aac cag gat ttc atc cag ctg gat 883 Pro Gln Gly Gly Arg Lys His Pro Asn Gln Asp Phe Ile Gln Leu Asp 250 255 260 acc acc aac att ttg ttc atc gtt gct ggt gcg ttc tct ggt ctg gag 931 Thr Thr Asn Ile Leu Phe Ile Val Ala Gly Ala Phe Ser Gly Leu Glu 265 270 275 aag gtc atc gcg gac cgc aat ggc aag aaa ggc ttg ggc ttc ggt gtg 979 Lys Val Ile Ala Asp Arg Asn Gly Lys Lys Gly Leu Gly Phe Gly Val 280 285 290 gag gtc tct tcc aag aag gaa gaa gcc aac att gtg gat atc ttc aag 1027 Glu Val Ser Ser Lys Lys Glu Glu Ala Asn Ile Val Asp Ile Phe Lys 295 300 305 gat gtc ctc cct gag gac ctg gtg aag ttt ggt ctc atc cca gaa ttc 1075 Asp Val Leu Pro Glu Asp Leu Val Lys Phe Gly Leu Ile Pro Glu Phe 310 315 320 325 att ggg cgt ctg cca gtc gtt gcc acc gta tcc aac ctg gat cag aaa 1123 Ile Gly Arg Leu Pro Val Val Ala Thr Val Ser Asn Leu Asp Gln Lys 330 335 340 tct ctg gtc aag gtt ctc acg gag cct cgt aac tca ttg gtg aag cag 1171 Ser Leu Val Lys Val Leu Thr Glu Pro Arg Asn Ser Leu Val Lys Gln 345 350 355 tat cga cgt ctg ttt gaa atg gat gac gct gtg ttg acc ttt act gat 1219 Tyr Arg Arg Leu Phe Glu Met Asp Asp Ala Val Leu Thr Phe Thr Asp 360 365 370 gat gct ttg gag gag atc gct aat cag gca ctc gag cgc aaa act ggc 1267 Asp Ala Leu Glu Glu Ile Ala Asn Gln Ala Leu Glu Arg Lys Thr Gly 375 380 385 gcc cgt ggc ctg cgc gcg atc atg gaa gag atc ctg gtt ccg atc atg 1315 Ala Arg Gly Leu Arg Ala Ile Met Glu Glu Ile Leu Val Pro Ile Met 390 395 400 405 tat gac ctc cca gac cgt aaa gac gtt ggc gaa gtc atc atc aac ggt 1363 Tyr Asp Leu Pro Asp Arg Lys Asp Val Gly Glu Val Ile Ile Asn Gly 410 415 420 gcc gtt gcc cgt ggc gaa gcc gaa cca gag atg ttg gaa gct gtc gca 1411 Ala Val Ala Arg Gly Glu Ala Glu Pro Glu Met Leu Glu Ala Val Ala 425 430 435 gaa gaa aag acc gcg tagttggcag gagttatcac cggctttgcc 1456 Glu Glu Lys Thr Ala 440 <210> 50 <211> 442 <212> PRT <213> Corynebacterium glutamicum <400> 50 Leu Tyr Ala Val Asn Leu Phe Asn Arg Gly Ser Gly Ser His Ser Leu 1 5 10 15 Met Ala Arg Met Gln Glu Ser Ala Asp Leu Leu Lys Cys Ser Phe Cys 20 25 30 Gly Lys Ser Gln Lys Gln Val Lys Lys Leu Ile Ala Gly Gly Ala Val 35 40 45 Tyr Ile Cys Asp Glu Cys Ile Glu Leu Cys Asn Glu Ile Ile Glu Glu 50 55 60 Glu Leu Gly Gln Ala Gln His Asp Glu Gln Glu Arg Asn Glu Leu Pro 65 70 75 80 Lys Pro Ser Glu Ile Ser Ala Phe Leu Asp Thr Tyr Val Ile Gly Gln 85 90 95 Asp Pro Ala Lys Arg Ile Leu Ser Val Ala Val Tyr Asn His Tyr Lys 100 105 110 Arg Leu Arg Ala Ser Glu Thr Ile Gly Arg Arg Arg Asn Asp Glu Pro 115 120 125 Glu Thr Glu Leu Val Lys Ser Asn Ile Leu Met Leu Gly Pro Thr Gly 130 135 140 Ser Gly Lys Thr Phe Leu Ala Gln Thr Leu Ala Lys Leu Leu Asp Val 145 150 155 160 Pro Phe Ala Ile Ala Asp Ala Thr Ser Leu Thr Glu Ala Gly Tyr Val 165 170 175 Gly Glu Asp Val Glu Asn Ile Leu Leu Lys Leu Leu Gln Ala Ala Asp 180 185 190 Phe Asp Val Glu Arg Ala Gln Arg Gly Ile Ile Tyr Ile Asp Glu Val 195 200 205 Asp Lys Ile Ser Arg Lys Ser Glu Asn Pro Ser Ile Thr Arg Asp Val 210 215 220 Ser Gly Glu Gly Val Gln Gln Ala Leu Leu Lys Ile Leu Glu Gly Thr 225 230 235 240 Val Ala Ala Ile Pro Pro Gln Gly Gly Arg Lys His Pro Asn Gln Asp 245 250 255 Phe Ile Gln Leu Asp Thr Thr Asn Ile Leu Phe Ile Val Ala Gly Ala 260 265 270 Phe Ser Gly Leu Glu Lys Val Ile Ala Asp Arg Asn Gly Lys Lys Gly 275 280 285 Leu Gly Phe Gly Val Glu Val Ser Ser Lys Lys Glu Glu Ala Asn Ile 290 295 300 Val Asp Ile Phe Lys Asp Val Leu Pro Glu Asp Leu Val Lys Phe Gly 305 310 315 320 Leu Ile Pro Glu Phe Ile Gly Arg Leu Pro Val Val Ala Thr Val Ser 325 330 335 Asn Leu Asp Gln Lys Ser Leu Val Lys Val Leu Thr Glu Pro Arg Asn 340 345 350 Ser Leu Val Lys Gln Tyr Arg Arg Leu Phe Glu Met Asp Asp Ala Val 355 360 365 Leu Thr Phe Thr Asp Asp Ala Leu Glu Glu Ile Ala Asn Gln Ala Leu 370 375 380 Glu Arg Lys Thr Gly Ala Arg Gly Leu Arg Ala Ile Met Glu Glu Ile 385 390 395 400 Leu Val Pro Ile Met Tyr Asp Leu Pro Asp Arg Lys Asp Val Gly Glu 405 410 415 Val Ile Ile Asn Gly Ala Val Ala Arg Gly Glu Ala Glu Pro Glu Met 420 425 430 Leu Glu Ala Val Ala Glu Glu Lys Thr Ala 435 440 <210> 51 <211> 523 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (493) <223> RXA01182 <400> 51 gttaaaacgg aaactaatac cccaaaggat accgattcaa tttgtgatgt gtggtgttcg 60 ggtcatatca agctaaacag atgcccccta caataggctt gtg ttc aat tta ttt 115 Val Phe Asn Leu Phe 1 5 ggt cgt aaa act cct cgc tct aac ctc cgc cca cca cgc ggt ccg ggc 163 Gly Arg Lys Thr Pro Arg Ser Asn Leu Arg Pro Pro Arg Gly Pro Gly 10 15 20 gat act gtg cgc ccg gaa gat tta aaa ttc ttg atg caa tgg gtg cag 211 Asp Thr Val Arg Pro Glu Asp Leu Lys Phe Leu Met Gln Trp Val Gln 25 30 35 gat aag cca ttt gtt gag gca ttc gtt gaa ccg gaa acg ctg gtc aat 259 Asp Lys Pro Phe Val Glu Ala Phe Val Glu Pro Glu Thr Leu Val Asn 40 45 50 gag atg tct gtc gtt ttg gtt gat gct cat ggg gtt ttt gtc cgc cga 307 Glu Met Ser Val Val Leu Val Asp Ala His Gly Val Phe Val Arg Arg 55 60 65 agg atc ggc ggt ccc aaa ggg att gat gtt atc gcg aaa aag ctc ggc 355 Arg Ile Gly Gly Pro Lys Gly Ile Asp Val Ile Ala Lys Lys Leu Gly 70 75 80 85 gtt ccg gtt tat gat gtt gag gag acc ggt tac ccc caa agg atg cgc 403 Val Pro Val Tyr Asp Val Glu Glu Thr Gly Tyr Pro Gln Arg Met Arg 90 95 100 gaa cgc att gaa tat gag cgc atc tta aga aag cgt gag gaa caa aaa 451 Glu Arg Ile Glu Tyr Glu Arg Ile Leu Arg Lys Arg Glu Glu Gln Lys 105 110 115 gct cgc cgc gct aaa ttt gag cgc ggc gag aat cct gat ctt 493 Ala Arg Arg Ala Lys Phe Glu Arg Gly Glu Asn Pro Asp Leu 120 125 130 taactagcgt ttagctttcc gacagatcct 523 <210> 52 <211> 131 <212> PRT <213> Corynebacterium glutamicum <400> 52 Val Phe Asn Leu Phe Gly Arg Lys Thr Pro Arg Ser Asn Leu Arg Pro 1 5 10 15 Pro Arg Gly Pro Gly Asp Thr Val Arg Pro Glu Asp Leu Lys Phe Leu 20 25 30 Met Gln Trp Val Gln Asp Lys Pro Phe Val Glu Ala Phe Val Glu Pro 35 40 45 Glu Thr Leu Val Asn Glu Met Ser Val Val Leu Val Asp Ala His Gly 50 55 60 Val Phe Val Arg Arg Arg Ile Gly Gly Pro Lys Gly Ile Asp Val Ile 65 70 75 80 Ala Lys Lys Leu Gly Val Pro Val Tyr Asp Val Glu Glu Thr Gly Tyr 85 90 95 Pro Gln Arg Met Arg Glu Arg Ile Glu Tyr Glu Arg Ile Leu Arg Lys 100 105 110 Arg Glu Glu Gln Lys Ala Arg Arg Ala Lys Phe Glu Arg Gly Glu Asn 115 120 125 Pro Asp Leu 130 <210> 53 <211> 1606 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1576) <223> RXA01214 <400> 53 ggtgtttatg aaaatttgtt tgagggagtg aaggcgcatg ttgttgccag agttgaatcg 60 tcggactttt ttcaaagggg ccggggtgct ggcagcaacg gtg gtg ggt gcg cag 115 Val Val Gly Ala Gln 1 5 gtg ctg gtg gcg tgt tcc tca gat gat gtg cgt ggt tat ggg gga gag 163 Val Leu Val Ala Cys Ser Ser Asp Asp Val Arg Gly Tyr Gly Gly Glu 10 15 20 ccg cgg acg ttg cct att cca cca gca gat tta ggt acg cgt gag gga 211 Pro Arg Thr Leu Pro Ile Pro Pro Ala Asp Leu Gly Thr Arg Glu Gly 25 30 35 tct agc gtg cac ttt gcc ctg gag gct cag act ggg gag agt cag att 259 Ser Ser Val His Phe Ala Leu Glu Ala Gln Thr Gly Glu Ser Gln Ile 40 45 50 ttg ccg gat gtc aca acg aag acg tgg ggt ttc aat ggc act cat ttg 307 Leu Pro Asp Val Thr Thr Lys Thr Trp Gly Phe Asn Gly Thr His Leu 55 60 65 ggg ccg acg ttg gtg gtg aag aaa ggt gat gac gtc cac gtt gat gtg 355 Gly Pro Thr Leu Val Val Lys Lys Gly Asp Asp Val His Val Asp Val 70 75 80 85 ata aac aat ttg gat gaa atg acc act gtg cac tgg cat ggc atg aag 403 Ile Asn Asn Leu Asp Glu Met Thr Thr Val Val His Trp His Gly Met Lys 90 95 100 ttg ccg gcg att gct gat ggt ggt ccg cac tca ccg atc ggg cct ggg 451 Leu Pro Ala Ile Ala Asp Gly Gly Pro His Ser Pro Ile Gly Pro Gly 105 110 115 cag acg tgg tca cca acg tgg act gtg gcc aat gat gca gcc act ttg 499 Gln Thr Trp Ser Pro Thr Trp Thr Val Ala Asn Asp Ala Ala Thr Leu 120 125 130 tgg tac cac ccg cac act cat ggc ctg aca ggt ttg cat gcg tac cgt 547 Trp Tyr His Pro His Thr His Gly Leu Thr Gly Leu His Ala Tyr Arg 135 140 145 ggt ttg gcg ggg atg atc att gtg gaa gat gaa gca aca gac aag ctg 595 Gly Leu Ala Gly Met Ile Ile Val Glu Asp Glu Ala Thr Asp Lys Leu 150 155 160 165 gat ctg cca cgc gag tac agt gtg gac gat att ccg ctg gtt tta atg 643 Asp Leu Pro Arg Glu Tyr Ser Val Asp Asp Ile Pro Leu Val Leu Met 170 175 180 gat cac cgc ttc tta gaa gac ggt tcc ctt gat gag gaa gac ctc ccc 691 Asp His Arg Phe Leu Glu Asp Gly Ser Leu Asp Glu Glu Asp Leu Pro 185 190 195 gat ctt ggg ctg ttg ggc gat acc ccc act gcc aat ggc att acc aat 739 Asp Leu Gly Leu Leu Gly Asp Thr Pro Thr Ala Asn Gly Ile Thr Asn 200 205 210 gcg cac ttt gat gcc acc acg cgc cgg gtt cgg ttc cgc gtg ctc aac 787 Ala His Phe Asp Ala Thr Thr Arg Arg Val Arg Phe Arg Val Leu Asn 215 220 225 ggc tcc aat atg cgg ttc tat aac ttg gcg ttt tca gac acg cgc acc 835 Gly Ser Asn Met Arg Phe Tyr Asn Leu Ala Phe Ser Asp Thr Arg Thr 230 235 240 245 ttc caa gtc att gcc agc gat tcc ggt ttg ctg gat gaa cct caa gac 883 Phe Gln Val Ile Ala Ser Asp Ser Gly Leu Leu Asp Glu Pro Gln Asp 250 255 260 cgc acc acc ttg gct att ggc cca ggc gag cgg tgg gaa atc gtc gtg 931 Arg Thr Thr Leu Ala Ile Gly Pro Gly Glu Arg Trp Glu Ile Val Val 265 270 275 gag cta gag ccc ggc gag gac gtc acc ttg gaa tct gta ggt ttt gag 979 Glu Leu Glu Pro Gly Glu Asp Val Thr Leu Glu Ser Val Gly Phe Glu 280 285 290 gac aac tac ggc gtc cct gat gat gag ttc gtg ccc gat ttc ggc atg 1027 Asp Asn Tyr Gly Val Pro Asp Asp Glu Phe Val Pro Asp Phe Gly Met 295 300 305 tca gat tcc ttc cag ctg ctc acc atc acc ggc cct tcc gat gat gct 1075 Ser Asp Ser Phe Gln Leu Leu Thr Ile Thr Gly Pro Ser Asp Asp Ala 310 315 320 325 gcg caa gca cct gct ttg ccg ggc gtg ctg gtg aaa tcc acc gaa cct 1123 Ala Gln Ala Pro Ala Leu Pro Gly Val Leu Val Lys Ser Thr Glu Pro 330 335 340 gac gtc atc gat gcc act gaa cgc acc ttc atc atg aac acc ttc tcc 1171 Asp Val Ile Asp Ala Thr Glu Arg Thr Phe Ile Met Asn Thr Phe Ser 345 350 355 atc aac gat cta cag atg gac atg cag cgc gtt gac gtg gtg att gac 1219 Ile Asn Asp Leu Gln Met Asp Met Gln Arg Val Asp Val Val Ile Asp 360 365 370 cat gac cag cca gaa gtg tgg att gtc acc aac gac aac tcc gac tgg 1267 His Asp Gln Pro Glu Val Trp Ile Val Thr Asn Asp Asn Ser Asp Trp 375 380 385 ccc cac aac ttc cat gtc cac gac gcc cgg ttt aag gtg ctg aaa ttt 1315 Pro His Asn Phe His Val His Asp Ala Arg Phe Lys Val Leu Lys Phe 390 395 400 405 gaa ggc acc gac gta gag ctc ttc aac gac ggc tgg aaa gac acc gtc 1363 Glu Gly Thr Asp Val Glu Leu Phe Asn Asp Gly Trp Lys Asp Thr Val 410 415 420 ggc ctg cca ccg gga gca acc gca act tta gcc gtg gaa ttt ggc cac 1411 Gly Leu Pro Pro Gly Ala Thr Ala Thr Leu Ala Val Glu Phe Gly His 425 430 435 tac cca gac ccg caa tgg ccc tac atg tat cac tgc cac atg ctc tac 1459 Tyr Pro Asp Pro Gln Trp Pro Tyr Met Tyr His Cys His Met Leu Tyr 440 445 450 cac gag gat caa ggc atg atg ggg cag ttc gtc atc gtg gag cca ggc 1507 His Glu Asp Gln Gly Met Met Gly Gln Phe Val Ile Val Glu Pro Gly 455 460 465 gac gag ccg gcg gcg gtg ctg ggg tcg ggc acg ggc tcc agc att gac 1555 Asp Glu Pro Ala Ala Val Leu Gly Ser Gly Thr Gly Ser Ser Ile Asp 470 475 480 485 tcc gcc ggc gga cat gcg cac taggggcgtg gggcggcgtc gataagcgag 1606 Ser Ala Gly Gly His Ala His 490 <210> 54 <211> 492 <212> PRT <213> Corynebacterium glutamicum <400> 54 Val Val Gly Ala Gln Val Leu Val Ala Cys Ser Ser Asp Asp Val Arg 1 5 10 15 Gly Tyr Gly Gly Glu Pro Arg Thr Leu Pro Ile Pro Pro Ala Asp Leu 20 25 30 Gly Thr Arg Glu Gly Ser Ser Val His Phe Ala Leu Glu Ala Gln Thr 35 40 45 Gly Glu Ser Gln Ile Leu Pro Asp Val Thr Thr Lys Thr Trp Gly Phe 50 55 60 Asn Gly Thr His Leu Gly Pro Thr Leu Val Val Lys Lys Gly Asp Asp 65 70 75 80 Val His Val Asp Val Ile Asn Asn Leu Asp Glu Met Thr Thr Val His 85 90 95 Trp His Gly Met Lys Leu Pro Ala Ile Ala Asp Gly Gly Pro His Ser 100 105 110 Pro Ile Gly Pro Gly Gln Thr Trp Ser Pro Thr Trp Thr Val Ala Asn 115 120 125 Asp Ala Ala Thr Leu Trp Tyr His Pro His Thr His Gly Leu Thr Gly 130 135 140 Leu His Ala Tyr Arg Gly Leu Ala Gly Met Ile Ile Val Glu Asp Glu 145 150 155 160 Ala Thr Asp Lys Leu Asp Leu Pro Arg Glu Tyr Ser Val Asp Asp Ile 165 170 175 Pro Leu Val Leu Met Asp His Arg Phe Leu Glu Asp Gly Ser Leu Asp 180 185 190 Glu Glu Asp Leu Pro Asp Leu Gly Leu Leu Gly Asp Thr Pro Thr Ala 195 200 205 Asn Gly Ile Thr Asn Ala His Phe Asp Ala Thr Thr Arg Arg Val Arg 210 215 220 Phe Arg Val Leu Asn Gly Ser Asn Met Arg Phe Tyr Asn Leu Ala Phe 225 230 235 240 Ser Asp Thr Arg Thr Phe Gln Val Ile Ala Ser Asp Ser Gly Leu Leu 245 250 255 Asp Glu Pro Gln Asp Arg Thr Thr Leu Ala Ile Gly Pro Gly Glu Arg 260 265 270 Trp Glu Ile Val Val Glu Leu Glu Pro Gly Glu Asp Val Thr Leu Glu 275 280 285 Ser Val Gly Phe Glu Asp Asn Tyr Gly Val Pro Asp Asp Glu Phe Val 290 295 300 Pro Asp Phe Gly Met Ser Asp Ser Phe Gln Leu Leu Thr Ile Thr Gly 305 310 315 320 Pro Ser Asp Asp Ala Ala Gln Ala Pro Ala Leu Pro Gly Val Leu Val 325 330 335 Lys Ser Thr Glu Pro Asp Val Ile Asp Ala Thr Glu Arg Thr Phe Ile 340 345 350 Met Asn Thr Phe Ser Ile Asn Asp Leu Gln Met Asp Met Gln Arg Val 355 360 365 Asp Val Val Ile Asp His Asp Gln Pro Glu Val Trp Ile Val Thr Asn 370 375 380 Asp Asn Ser Asp Trp Pro His Asn Phe His Val His Asp Ala Arg Phe 385 390 395 400 Lys Val Leu Lys Phe Glu Gly Thr Asp Val Glu Leu Phe Asn Asp Gly 405 410 415 Trp Lys Asp Thr Val Gly Leu Pro Pro Gly Ala Thr Ala Thr Leu Ala 420 425 430 Val Glu Phe Gly His Tyr Pro Asp Pro Gln Trp Pro Tyr Met Tyr His 435 440 445 Cys His Met Leu Tyr His Glu Asp Gln Gly Met Met Gly Gln Phe Val 450 455 460 Ile Val Glu Pro Gly Asp Glu Pro Ala Ala Val Leu Gly Ser Gly Thr 465 470 475 480 Gly Ser Ser Ile Asp Ser Ala Gly Gly His Ala His 485 490 <210> 55 <211> 1153 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (1123) <223> RXA01224 <400> 55 ttggcggcgg gaagttcagg cttgggggca aacagtgctt ggattttaga caaaaaactc 60 acggaagtca tcctatggca ggcgcgccta ggatggtgcc atg agc atc ctt gac 115 Met Ser Ile Leu Asp 1 5 acg ttg aaa act ccc gtg att gtc gcc ccg atg gct ggc ggc ccg tcc 163 Thr Leu Lys Thr Pro Val Ile Val Ala Pro Met Ala Gly Gly Pro Ser 10 15 20 act ccc gcg ttg gtc aat gca gca gca gag gca ggt tcc ctc ggg ttc 211 Thr Pro Ala Leu Val Asn Ala Ala Ala Glu Ala Gly Ser Leu Gly Phe 25 30 35 ttg gct ggt ggc gtc atg cct ctt gag cag ctg aaa cag gaa ttg tca 259 Leu Ala Gly Gly Val Met Pro Leu Glu Gln Leu Lys Gln Glu Leu Ser 40 45 50 gag gta aaa ggc gtc ttt ggc gtc aac ctg ttt cgc ccg cag acg gat 307 Glu Val Lys Gly Val Phe Gly Val Asn Leu Phe Arg Pro Gln Thr Asp 55 60 65 gcg cct aag cct tca gac att gat gag ctg gcg gga ttg ttg tcc tcg 355 Ala Pro Lys Pro Ser Asp Ile Asp Glu Leu Ala Gly Leu Leu Ser Ser 70 75 80 85 gcg ttt cgg caa ttt ggc ctc gat gag ccg acg gtg cct acg ccg gat 403 Ala Phe Arg Gln Phe Gly Leu Asp Glu Pro Thr Val Pro Thr Pro Asp 90 95 100 ttg agc aat ggg tgg gag gct aaa ttt gag gcc gtt ctt gcc gct aag 451 Leu Ser Asn Gly Trp Glu Ala Lys Phe Glu Ala Val Leu Ala Ala Lys 105 110 115 ccc gcc gtt ttt tcc tgc acc ttt ggt att ttt agc gct gaa gaa ttc 499 Pro Ala Val Phe Ser Cys Thr Phe Gly Ile Phe Ser Ala Glu Glu Phe 120 125 130 gcc cgg atc aaa gcc acc gga att gag gcg tgg gtg acg gtg acc aat 547 Ala Arg Ile Lys Ala Thr Gly Ile Glu Ala Trp Val Thr Val Thr Asn 135 140 145 ccg gag gac gcg ctg gct gcg cag aaa gct ggc gcc aac gcg ctt gtc 595 Pro Glu Asp Ala Leu Ala Ala Gln Lys Ala Gly Ala Asn Ala Leu Val 150 155 160 165 gtg caa ggc ccc gag gcg ggt ggg cac cgc tct acc tgg tcc att gaa 643 Val Gln Gly Pro Glu Ala Gly Gly His Arg Ser Thr Trp Ser Ile Glu 170 175 180 gtg gag ccg gac gag cgc gac ctg aaa acc ctc ctc gca gct gtc aaa 691 Val Glu Pro Asp Glu Arg Asp Leu Lys Thr Leu Leu Ala Ala Val Lys 185 190 195 caa gcg ggc gtt tac ctc ccg ctc atc gca gcc ggc ggc ctt tca acc 739 Gln Ala Gly Val Tyr Leu Pro Leu Ile Ala Ala Gly Gly Leu Ser Thr 200 205 210 tcc gca gac gtg gca gca att tta gaa gcc ggc gcc agc gct gcc tcc 787 Ser Ala Asp Val Ala Ala Ile Leu Glu Ala Gly Ala Ser Ala Ala Ser 215 220 225 tgt ggt tcc gcc ttt ttg ctt agc gac gaa gcc ggc acc agc tca ctt 835 Cys Gly Ser Ala Phe Leu Leu Ser Asp Glu Ala Gly Thr Ser Ser Leu 230 235 240 245 aac cgc gag atc ttg gac gcc gcc cca gca ctt ggt ttg gaa tcg gtg 883 Asn Arg Glu Ile Leu Asp Ala Ala Pro Ala Leu Gly Leu Glu Ser Val 250 255 260 tca tct cgc gca ttt tcg ggc cgt tat gcc agg gga gtg gaa acc agg 931 Ser Ser Arg Ala Phe Ser Gly Arg Tyr Ala Arg Gly Val Glu Thr Arg 265 270 275 ttc acc cgt tcg aac gag ggg tta ccc ccg ttg tac cca tac ctc aac 979 Phe Thr Arg Ser Asn Glu Gly Leu Pro Pro Leu Tyr Pro Tyr Leu Asn 280 285 290 cca atg atc aca tct tta cgt aag gtg gcg gga agt gca ggg aac tgg 1027 Pro Met Ile Thr Ser Leu Arg Lys Val Ala Gly Ser Ala Gly Asn Trp 295 300 305 gat tac gcc tac tgc ctg gta gga gtc ggc ctg gaa tcg att gcg aag 1075 Asp Tyr Ala Tyr Cys Leu Val Gly Val Gly Leu Glu Ser Ile Ala Lys 310 315 320 325 ggt agt gca aag cag ata ctg gaa tca tta aca cct tcc gct ttg ggc 1123 Gly Ser Ala Lys Gln Ile Leu Glu Ser Leu Thr Pro Ser Ala Leu Gly 330 335 340 taatgttggg gggagtgctt tcaactatcc 1153 <210> 56 <211> 341 <212> PRT <213> Corynebacterium glutamicum <400> 56 Met Ser Ile Leu Asp Thr Leu Lys Thr Pro Val Ile Val Ala Pro Met 1 5 10 15 Ala Gly Gly Pro Ser Thr Pro Ala Leu Val Asn Ala Ala Ala Glu Ala 20 25 30 Gly Ser Leu Gly Phe Leu Ala Gly Gly Val Met Pro Leu Glu Gln Leu 35 40 45 Lys Gln Glu Leu Ser Glu Val Lys Gly Val Phe Gly Val Asn Leu Phe 50 55 60 Arg Pro Gln Thr Asp Ala Pro Lys Pro Ser Asp Ile Asp Glu Leu Ala 65 70 75 80 Gly Leu Leu Ser Ser Ala Phe Arg Gln Phe Gly Leu Asp Glu Pro Thr 85 90 95 Val Pro Thr Pro Asp Leu Ser Asn Gly Trp Glu Ala Lys Phe Glu Ala 100 105 110 Val Leu Ala Ala Lys Pro Ala Val Phe Ser Cys Thr Phe Gly Ile Phe 115 120 125 Ser Ala Glu Glu Phe Ala Arg Ile Lys Ala Thr Gly Ile Glu Ala Trp 130 135 140 Val Thr Val Thr Asn Pro Glu Asp Ala Leu Ala Ala Gln Lys Ala Gly 145 150 155 160 Ala Asn Ala Leu Val Val Gln Gly Pro Glu Ala Gly Gly His Arg Ser 165 170 175 Thr Trp Ser Ile Glu Val Glu Pro Asp Glu Arg Asp Leu Lys Thr Leu 180 185 190 Leu Ala Ala Val Lys Gln Ala Gly Val Tyr Leu Pro Leu Ile Ala Ala 195 200 205 Gly Gly Leu Ser Thr Ser Ala Asp Val Ala Ala Ile Leu Glu Ala Gly 210 215 220 Ala Ser Ala Ala Ser Cys Gly Ser Ala Phe Leu Leu Ser Asp Glu Ala 225 230 235 240 Gly Thr Ser Ser Leu Asn Arg Glu Ile Leu Asp Ala Ala Pro Ala Leu 245 250 255 Gly Leu Glu Ser Val Ser Ser Arg Ala Phe Ser Gly Arg Tyr Ala Arg 260 265 270 Gly Val Glu Thr Arg Phe Thr Arg Ser Asn Glu Gly Leu Pro Pro Leu 275 280 285 Tyr Pro Tyr Leu Asn Pro Met Ile Thr Ser Leu Arg Lys Val Ala Gly 290 295 300 Ser Ala Gly Asn Trp Asp Tyr Ala Tyr Cys Leu Val Gly Val Gly Leu 305 310 315 320 Glu Ser Ile Ala Lys Gly Ser Ala Lys Gln Ile Leu Glu Ser Leu Thr 325 330 335 Pro Ser Ala Leu Gly 340 <210> 57 <211> 2248 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (2218) <223> RXA01277 <400> 57 gaccagccga atctacattc cttattctgc tggcgttaca attcagggcc aaacccgtat 60 gatgaaaaag acaccgggga aatcggagtg cgcgtagatt ttg aaa acg gcc ggt 115 Leu Lys Thr Ala Gly 1 5 act act cgg ttc acg ttt acg tcg gct gat cca att gga ggc gcc ctc 163 Thr Thr Arg Phe Thr Phe Thr Ser Ala Asp Pro Ile Gly Gly Ala Leu 10 15 20 gga agc cgc ctt aaa aaa cct gcc ggt caa aag atc act aac ctg aac 211 Gly Ser Arg Leu Lys Lys Pro Ala Gly Gln Lys Ile Thr Asn Leu Asn 25 30 35 ttc atg act gat tac acg ttc ctc gaa gac att gac acc ccg gaa gcg 259 Phe Met Thr Asp Tyr Thr Phe Leu Glu Asp Ile Asp Thr Pro Glu Ala 40 45 50 ctc gcg tgg gcg gaa aaa tgg tcg ggg gaa agc gtc gaa aag cta aaa 307 Leu Ala Trp Ala Glu Lys Trp Ser Gly Glu Ser Val Glu Lys Leu Lys 55 60 65 agc cca gcc aag gac gcc ctg gaa gcc agg ctg ctg gct gcg ttg gac 355 Ser Pro Ala Lys Asp Ala Leu Glu Ala Arg Leu Leu Ala Ala Leu Asp 70 75 80 85 acc gat gat cgc att gcc tac gtg agc cgg cgc ggt gag aag ctg tac 403 Thr Asp Asp Arg Ile Ala Tyr Val Ser Arg Arg Gly Glu Lys Leu Tyr 90 95 100 aac ttt tgg cgg gac gcg cag cat ccg cgt gga gtg tgg cgc acg acc 451 Asn Phe Trp Arg Asp Ala Gln His Pro Arg Gly Val Trp Arg Thr Thr 105 110 115 acg ttg gag tcg tat gaa agt gac cag ccg gag tgg gac gtg ctc att 499 Thr Leu Glu Ser Tyr Glu Ser Asp Gln Pro Glu Trp Asp Val Leu Ile 120 125 130 gat gtg gat gcg ttg gcg gag gat gag ggc gaa aac tgg gta tgg aag 547 Asp Val Asp Ala Leu Ala Glu Asp Glu Gly Glu Asn Trp Val Trp Lys 135 140 145 ggc gcg gtt gtg cgc tcg ccg gag ttt gat cgg gcg ttg gtg aag ttc 595 Gly Ala Val Val Arg Ser Pro Glu Phe Asp Arg Ala Leu Val Lys Phe 150 155 160 165 tcg cgg ggc ggg gct gat gcg acg gtg att agg gag ttt gat ctg gcc 643 Ser Arg Gly Gly Ala Asp Ala Thr Val Ile Arg Glu Phe Asp Leu Ala 170 175 180 acg gct gct ttc gtg gat gat tcg ccg ttt gaa ttg gag gag gcg aag 691 Thr Ala Ala Phe Val Asp Asp Ser Pro Phe Glu Leu Glu Glu Ala Lys 185 190 195 tcc gat gtc acg tgg gtt gat ctg gat acg ttg ctg gtg ggc acg gat 739 Ser Asp Val Thr Trp Val Asp Leu Asp Thr Leu Leu Val Gly Thr Asp 200 205 210 acc ggc gag ggg tca ctg acg gat tct ggg tac ccg gcg cgg gtg ctc 787 Thr Gly Glu Gly Ser Leu Thr Asp Ser Gly Tyr Pro Ala Arg Val Leu 215 220 225 acg tgg aag cgt ggg act ccg ctt gag cag gcg gag ttg ttc ttt gag 835 Thr Trp Lys Arg Gly Thr Pro Leu Glu Gln Ala Glu Leu Phe Phe Glu 230 235 240 245 ggg tcg cgt cag gat gtg gcg act cat gcg tgg cgg gat tca aca cct 883 Gly Ser Arg Gln Asp Val Ala Thr His Ala Trp Arg Asp Ser Thr Pro 250 255 260 ggt ttt gag cgg acg ttt gtg tca agg tcg ttg gat ttc tat aat tcg 931 Gly Phe Glu Arg Thr Phe Val Ser Arg Ser Leu Asp Phe Tyr Asn Ser 265 270 275 gag acg tcg ctg gaa acc gag ggt ggc ctg gtc aag ctt gat gtg ccg 979 Glu Thr Ser Leu Glu Thr Glu Gly Gly Leu Val Lys Leu Asp Val Pro 280 285 290 acc gat tgc gat gtc att gtg aag aag cag tgg att ttt gtg agt cct 1027 Thr Asp Cys Asp Val Ile Val Lys Lys Gln Trp Ile Phe Val Ser Pro 295 300 305 cgg acg gat ttc gct ggg att cca gca ggt ggc ttg gga gtg ctg ctg 1075 Arg Thr Asp Phe Ala Gly Ile Pro Ala Gly Gly Leu Gly Val Leu Leu 310 315 320 325 tta aag gag ttc ctt gag ggc ggg cgc gat ttt cag cct gtg ttt acg 1123 Leu Lys Glu Phe Leu Glu Gly Gly Arg Asp Phe Gln Pro Val Phe Thr 330 335 340 cct act gag tcg acg tcg ctg cag gga ttg gcc acg aca aag aat ttc 1171 Pro Thr Glu Ser Thr Ser Leu Gln Gly Leu Ala Thr Thr Lys Asn Phe 345 350 355 ctg gtt tta acg ctc ctt aat aat gtc tcc aca gaa atc gtc aca gtg 1219 Leu Val Leu Thr Leu Leu Asn Asn Val Ser Thr Glu Ile Val Thr Val 360 365 370 ccg ctc aat gat ccg aca acg gag cat gaa cac att gac ctc cca gag 1267 Pro Leu Asn Asp Pro Thr Thr Glu His Glu His Ile Asp Leu Pro Glu 375 380 385 cat gtc acc gcg cat gtg gtt gct acc tcc ccg ttg gat ggc gat gaa 1315 His Val Thr Ala His Val Val Ala Thr Ser Pro Leu Asp Gly Asp Glu 390 395 400 405 att tgg gtg cag gca gcg agt ttc acc gaa gcg cca acg ttg ctg cgt 1363 Ile Trp Val Gln Ala Ala Ser Phe Thr Glu Ala Pro Thr Leu Leu Arg 410 415 420 gcg gag ctg cct ggt gcg ctt gag gct gtg aag aag gcg ccg ttg cag 1411 Ala Glu Leu Pro Gly Ala Leu Glu Ala Val Lys Lys Ala Pro Leu Gln 425 430 435 ttt gaa aat gct ggt cag gag act cgt cag cat tgg gca acc tcg gcg 1459 Phe Glu Asn Ala Gly Gln Glu Thr Arg Gln His Trp Ala Thr Ser Ala 440 445 450 gat gga acg aag att ccg tac ttt att aca gga gcc ttc gag gag gaa 1507 Asp Gly Thr Lys Ile Pro Tyr Phe Ile Thr Gly Ala Phe Glu Glu Glu 455 460 465 cca caa aac acc ctg gtc cac gcc tac ggc ggc ttc gag gtt tcc ctt 1555 Pro Gln Asn Thr Leu Val His Ala Tyr Gly Gly Phe Glu Val Ser Leu 470 475 480 485 acc cca agc cac tcc ccg acc cgc ggc atc gca tgg ttg gaa aag ggc 1603 Thr Pro Ser His Ser Pro Thr Arg Gly Ile Ala Trp Leu Glu Lys Gly 490 495 500 tac tac ttt gtg gaa gcc aac ctg cgt ggt ggc ggt gaa ttc ggt ccg 1651 Tyr Tyr Phe Val Glu Ala Asn Leu Arg Gly Gly Gly Glu Phe Gly Pro 505 510 515 gaa tgg cat tcg cag gca acc aag ctg aac cgc atg aag gtg tgg gag 1699 Glu Trp His Ser Gln Ala Thr Lys Leu Asn Arg Met Lys Val Trp Glu 520 525 530 gat cac cgc gcg gtg ctc gcc gac ctt gtg gag cgc ggc tac gca acg 1747 Asp His Arg Ala Val Leu Ala Asp Leu Val Glu Arg Gly Tyr Ala Thr 535 540 545 ccg gag cag att gcg att cgt ggc gga tcc aac ggt ggt ttg ctg aca 1795 Pro Glu Gln Ile Ala Ile Arg Gly Gly Ser Asn Gly Gly Leu Leu Thr 550 555 560 565 agt ggc gcg tta act cag tac cca gaa gca ttc ggt gcg gca gtt gtg 1843 Ser Gly Ala Leu Thr Gln Tyr Pro Glu Ala Phe Gly Ala Ala Val Val 570 575 580 cag gtg ccg ttg gct gat atg ttg cgc tat cac acc tgg tca gcg ggt 1891 Gln Val Pro Leu Ala Asp Met Leu Arg Tyr His Thr Trp Ser Ala Gly 585 590 595 gct tcg tgg atg gcg gag tac ggc aac cct gac gat ccg gag gaa cgg 1939 Ala Ser Trp Met Ala Glu Tyr Gly Asn Pro Asp Asp Pro Glu Glu Arg 600 605 610 gcg gtg att gag cag tac tcg ccg gtg cag gcg gtg gtg ggc gtc gag 1987 Ala Val Ile Glu Gln Tyr Ser Pro Val Gln Ala Val Val Gly Val Glu 615 620 625 aag cga att tat cca ccc gca ttg gtg acg acc tca acc cgg gac gac 2035 Lys Arg Ile Tyr Pro Pro Ala Leu Val Thr Thr Ser Ser Ar Arg Asp Asp 630 635 640 645 cgc gtc cac ccc gcg cac gcg cgc ctt ttt gct caa gct ttg ctt gat 2083 Arg Val His Pro Ala His Ala Arg Leu Phe Ala Gln Ala Leu Leu Asp 650 655 660 gcg ggc cag gcc gtg gat tac tac gaa aac acc gag ggc ggc cat gcc 2131 Ala Gly Gln Ala Val Asp Tyr Tyr Glu Asn Thr Glu Gly Gly His Ala 665 670 675 ggc gcg gcg gat aac aag cag acc gcg ttt gtg gaa tcg ctg atc tac 2179 Gly Ala Ala Asp Asn Lys Gln Thr Ala Phe Val Glu Ser Leu Ile Tyr 680 685 690 acc tgg atc gag aag act ttg gat cag cag ggt agc att taatacctat 2228 Thr Trp Ile Glu Lys Thr Leu Asp Gln Gln Gly Ser Ile 695 700 705 gattatgcga aggctgcgct 2248 <210> 58 <211> 706 <212> PRT <213> Corynebacterium glutamicum <400> 58 Leu Lys Thr Ala Gly Thr Thr Arg Phe Thr Phe Thr Ser Ala Asp Pro 1 5 10 15 Ile Gly Gly Ala Leu Gly Ser Arg Leu Lys Lys Pro Ala Gly Gln Lys 20 25 30 Ile Thr Asn Leu Asn Phe Met Thr Asp Tyr Thr Phe Leu Glu Asp Ile 35 40 45 Asp Thr Pro Glu Ala Leu Ala Trp Ala Glu Lys Trp Ser Gly Glu Ser 50 55 60 Val Glu Lys Leu Lys Ser Pro Ala Lys Asp Ala Leu Glu Ala Arg Leu 65 70 75 80 Leu Ala Ala Leu Asp Thr Asp Asp Arg Ile Ala Tyr Val Ser Arg Arg 85 90 95 Gly Glu Lys Leu Tyr Asn Phe Trp Arg Asp Ala Gln His Pro Arg Gly 100 105 110 Val Trp Arg Thr Thr Thr Leu Glu Ser Tyr Glu Ser Asp Gln Pro Glu 115 120 125 Trp Asp Val Leu Ile Asp Val Asp Ala Leu Ala Glu Asp Glu Gly Glu 130 135 140 Asn Trp Val Trp Lys Gly Ala Val Val Arg Ser Pro Glu Phe Asp Arg 145 150 155 160 Ala Leu Val Lys Phe Ser Arg Gly Gly Ala Asp Ala Thr Val Ile Arg 165 170 175 Glu Phe Asp Leu Ala Thr Ala Ala Phe Val Asp Asp Ser Pro Phe Glu 180 185 190 Leu Glu Glu Ala Lys Ser Asp Val Thr Trp Val Asp Leu Asp Thr Leu 195 200 205 Leu Val Gly Thr Asp Thr Gly Glu Gly Ser Leu Thr Asp Ser Gly Tyr 210 215 220 Pro Ala Arg Val Leu Thr Trp Lys Arg Gly Thr Pro Leu Glu Gln Ala 225 230 235 240 Glu Leu Phe Phe Glu Gly Ser Arg Gln Asp Val Ala Thr His Ala Trp 245 250 255 Arg Asp Ser Thr Pro Gly Phe Glu Arg Thr Phe Val Ser Arg Ser Leu 260 265 270 Asp Phe Tyr Asn Ser Glu Thr Ser Leu Glu Thr Glu Gly Gly Leu Val 275 280 285 Lys Leu Asp Val Pro Thr Asp Cys Asp Val Ile Val Lys Lys Gln Trp 290 295 300 Ile Phe Val Ser Pro Arg Thr Asp Phe Ala Gly Ile Pro Ala Gly Gly 305 310 315 320 Leu Gly Val Leu Leu Leu Lys Glu Phe Leu Glu Gly Gly Arg Asp Phe 325 330 335 Gln Pro Val Phe Thr Pro Thr Glu Ser Thr Ser Leu Gln Gly Leu Ala 340 345 350 Thr Thr Lys Asn Phe Leu Val Leu Thr Leu Leu Asn Asn Val Ser Thr 355 360 365 Glu Ile Val Thr Val Pro Leu Asn Asp Pro Thr Thr Glu His Glu His 370 375 380 Ile Asp Leu Pro Glu His Val Thr Ala His Val Val Ala Thr Ser Pro 385 390 395 400 Leu Asp Gly Asp Glu Ile Trp Val Gln Ala Ala Ser Phe Thr Glu Ala 405 410 415 Pro Thr Leu Leu Arg Ala Glu Leu Pro Gly Ala Leu Glu Ala Val Lys 420 425 430 Lys Ala Pro Leu Gln Phe Glu Asn Ala Gly Gln Glu Thr Arg Gln His 435 440 445 Trp Ala Thr Ser Ala Asp Gly Thr Lys Ile Pro Tyr Phe Ile Thr Gly 450 455 460 Ala Phe Glu Glu Glu Pro Gln Asn Thr Leu Val His Ala Tyr Gly Gly 465 470 475 480 Phe Glu Val Ser Leu Thr Pro Ser His Ser Pro Thr Arg Gly Ile Ala 485 490 495 Trp Leu Glu Lys Gly Tyr Tyr Phe Val Glu Ala Asn Leu Arg Gly Gly 500 505 510 Gly Glu Phe Gly Pro Glu Trp His Ser Gln Ala Thr Lys Leu Asn Arg 515 520 525 Met Lys Val Trp Glu Asp His Arg Ala Val Leu Ala Asp Leu Val Glu 530 535 540 Arg Gly Tyr Ala Thr Pro Glu Gln Ile Ala Ile Arg Gly Gly Ser Asn 545 550 555 560 Gly Gly Leu Leu Thr Ser Gly Ala Leu Thr Gln Tyr Pro Glu Ala Phe 565 570 575 Gly Ala Ala Val Val Gln Val Pro Leu Ala Asp Met Leu Arg Tyr His 580 585 590 Thr Trp Ser Ala Gly Ala Ser Trp Met Ala Glu Tyr Gly Asn Pro Asp 595 600 605 Asp Pro Glu Glu Arg Ala Val Ile Glu Gln Tyr Ser Pro Val Gln Ala 610 615 620 Val Val Gly Val Glu Lys Arg Ile Tyr Pro Pro Ala Leu Val Thr Thr 625 630 635 640 Ser Thr Arg Asp Asp Arg Val His Pro Ala His Ala Arg Leu Phe Ala 645 650 655 Gln Ala Leu Leu Asp Ala Gly Gln Ala Val Asp Tyr Tyr Glu Asn Thr 660 665 670 Glu Gly Gly His Ala Gly Ala Ala Asp Asn Lys Gln Thr Ala Phe Val 675 680 685 Glu Ser Leu Ile Tyr Thr Trp Ile Glu Lys Thr Leu Asp Gln Gln Gly 690 695 700 Ser yle 705 <210> 59 <211> 3874 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (101) .. (3844) <223> RXA01302 <400> 59 tggggctgcg tggtgttctt catcatcgcc accgctttga cctggatcta ctacgcccgc 60 ccgaacgctc cattcccggg ataaaccgaa aggccaatcc atg act aca act act 115 Met Thr Thr Thr Thr 1 5 tct tct ggg aag tct tct gaa aag atc aac ccc ctc ttc aag ctc ggc 163 Ser Ser Gly Lys Ser Ser Glu Lys Ile Asn Pro Leu Phe Lys Leu Gly 10 15 20 agt ttc cta aga aaa ggc acc gtc ggt tct gaa ggc cag cag att ttc 211 Ser Phe Leu Arg Lys Gly Thr Val Gly Ser Glu Gly Gln Gln Ile Phe 25 30 35 ctt cag ggc gga cgc caa gcc gat gtg ttt tat cgc aac cga tgg gct 259 Leu Gln Gly Gly Arg Gln Ala Asp Val Phe Tyr Arg Asn Arg Trp Ala 40 45 50 ttc gat aaa gtc gtg cgc tcc aca cat ggc gtg aac tgc acg ggc tcc 307 Phe Asp Lys Val Val Arg Ser Thr His Gly Val Asn Cys Thr Gly Ser 55 60 65 tgc tcg tgg aaa gtg tat gta aaa gac ggt gtg atc acc tgg gaa tcc 355 Cys Ser Trp Lys Val Tyr Val Lys Asp Gly Val Ile Thr Trp Glu Ser 70 75 80 85 cag gca gtg gat tac cca act acc ggt gcg gat atg ccc gac tat gaa 403 Gln Ala Val Asp Tyr Pro Thr Thr Gly Ala Asp Met Pro Asp Tyr Glu 90 95 100 cca cgt ggc tgc cct cgt ggt gca tca ttt tcc tgg tac acc tac tcc 451 Pro Arg Gly Cys Pro Arg Gly Ala Ser Phe Ser Trp Tyr Thr Tyr Ser 105 110 115 cca acc cgc atc cgc tac cca tac atc cgt ggc gtg cta gtt gat atg 499 Pro Thr Arg Ile Arg Tyr Pro Tyr Ile Arg Gly Val Leu Val Asp Met 120 125 130 tac cgc gaa gcc aag gaa cgc ctg ggc gat ccg gtg ctg gcg tgg cgc 547 Tyr Arg Glu Ala Lys Glu Arg Leu Gly Asp Pro Val Leu Ala Trp Arg 135 140 145 gac att gta gaa acc cca gaa aag cgc aaa gca tat gta tcc cag cgg 595 Asp Ile Val Glu Thr Pro Glu Lys Arg Lys Ala Tyr Val Ser Gln Arg 150 155 160 165 ggc aaa ggt ggc ctc atc cgc gtt cag tat gag gaa gcc atg gag att 643 Gly Lys Gly Gly Leu Ile Arg Val Gln Tyr Glu Glu Ala Met Glu Ile 170 175 180 gct gcg gca gcc cat gtg tac acc atc cgc caa tac ggc ccc gac cgc 691 Ala Ala Ala Ala His Val Tyr Thr Ile Arg Gln Tyr Gly Pro Asp Arg 185 190 195 att cat gga ttc acc gtt att ccc gca atg tcg cag gtg tct tac ggt 739 Ile His Gly Phe Thr Val Ile Pro Ala Met Ser Gln Val Ser Tyr Gly 200 205 210 gct ggt act cgc ttc ttg cag atg atc ggc gga gtg gcg ctg tcc ttc 787 Ala Gly Thr Arg Phe Leu Gln Met Ile Gly Gly Val Ala Leu Ser Phe 215 220 225 tac gat tgg tac gcc gac ctc cca cca gca tca cca caa act ttc ggc 835 Tyr Asp Trp Tyr Ala Asp Leu Pro Pro Ala Ser Pro Gln Thr Phe Gly 230 235 240 245 gat caa act gac gtt ccg gaa tct ggc gac tgg tac aac tcc agc tac 883 Asp Gln Thr Asp Val Pro Glu Ser Gly Asp Trp Tyr Asn Ser Ser Tyr 250 255 260 ctc atg atg tgg ggt tcc aac att ccg gtg acc cgc acg cct gac tcc 931 Leu Met Met Trp Gly Ser Asn Ile Pro Val Thr Arg Thr Pro Asp Ser 265 270 275 cac ttc atg gtg gaa gcc cgc tac aag ggc acc aag gtt gtt gtg gtt 979 His Phe Met Val Glu Ala Arg Tyr Lys Gly Thr Lys Val Val Val Val 280 285 290 tcc ccg gat ttc gct gac tcc acc aaa ttt gct gat gaa tgg gca cgc 1027 Ser Pro Asp Phe Ala Asp Ser Thr Lys Phe Ala Asp Glu Trp Ala Arg 295 300 305 atc cac cct ggt act gac ggc gca ctc gcc ttt gcc atg ggc cat gtg 1075 Ile His Pro Gly Thr Asp Gly Ala Leu Ala Phe Ala Met Gly His Val 310 315 320 325 atc ttg aag gaa ttc cat gtt gac aag aag acg ccg tac ttc atg gac 1123 Ile Leu Lys Glu Phe His Val Asp Lys Lys Thr Pro Tyr Phe Met Asp 330 335 340 tac atg cgc aaa tac acg gac tct cct ttc ctc gtg gaa tta gat gag 1171 Tyr Met Arg Lys Tyr Thr Asp Ser Pro Phe Leu Val Glu Leu Asp Glu 345 350 355 cac ggc gat ggc acc tac acc cca ggt aaa ttc ctc act gca gac cgc 1219 His Gly Asp Gly Thr Tyr Thr Pro Gly Lys Phe Leu Thr Ala Asp Arg 360 365 370 gca gct gat atc tcc cca gcg ctt gcc gcc act cca aat gcc acc cac 1267 Ala Ala Asp Ile Ser Pro Ala Leu Ala Ala Thr Pro Asn Ala Thr His 375 380 385 cgt ctc ctt gtg ctg caa aaa gat ggc tca gtt gta gat ccc ggt ggc 1315 Arg Leu Leu Val Leu Gln Lys Asp Gly Ser Val Val Asp Pro Gly Gly 390 395 400 405 act gtc gcg gac cgt tgg ggt gaa gaa ggc atg ggt aag tgg aat ctg 1363 Thr Val Ala Asp Arg Trp Gly Glu Glu Gly Met Gly Lys Trp Asn Leu 410 415 420 cgc tta gac ggc gta gat cca gtg atg act att gca gat gta cag act 1411 Arg Leu Asp Gly Val Asp Pro Val Met Thr Ile Ala Asp Val Gln Thr 425 430 435 gac acc gaa act gcg gaa gtc ctc ttc ccc cgc ttc gat ctc cca gca 1459 Asp Thr Glu Thr Ala Glu Val Leu Phe Pro Arg Phe Asp Leu Pro Ala 440 445 450 act gcc acc caa gaa ggc ccc att ggt gct ggc acc atc agc cgg ggc 1507 Thr Ala Thr Gln Glu Gly Pro Ile Gly Ala Gly Thr Ile Ser Arg Gly 455 460 465 gtt ccc acc atc acg ttg aat ggc cga aag tac acc act gtc ttt gat 1555 Val Pro Thr Ile Thr Leu Asn Gly Arg Lys Tyr Thr Thr Val Phe Asp 470 475 480 485 gtg ttg ctc gca cac tac ggt gtg aac cgc gaa gag ctc aac ctt cct 1603 Val Leu Leu Ala His Tyr Gly Val Asn Arg Glu Glu Leu Asn Leu Pro 490 495 500 ggt gag tgg cct aag gat ttc cag gat cca gtc atg ggt act cct gcg 1651 Gly Glu Trp Pro Lys Asp Phe Gln Asp Pro Val Met Gly Thr Pro Ala 505 510 515 tgg cag gaa gag ctc acg ggt gtt cct gct aat cag gcg att cgt ttg 1699 Trp Gln Glu Glu Leu Thr Gly Val Pro Ala Asn Gln Ala Ile Arg Leu 520 525 530 ggt cgg gaa ttt gct cag aat gct gat gat tcc aag ggc cgt tcc cag 1747 Gly Arg Glu Phe Ala Gln Asn Ala Asp Asp Ser Lys Gly Arg Ser Gln 535 540 545 atc atc atg ggt gct ggt gtg aac cac tac ttc cat gcg gat tct att 1795 Ile Ile Met Gly Ala Gly Val Asn His Tyr Phe His Ala Asp Ser Ile 550 555 560 565 tat cgc aca ttc ttg gcg ctg acc tct atg tgt ggc acc caa ggt gtt 1843 Tyr Arg Thr Phe Leu Ala Leu Thr Ser Met Cys Gly Thr Gln Gly Val 570 575 580 aac ggt ggc ggt tgg gct cac tac gtt ggt cag gag aaa ctc cgt cca 1891 Asn Gly Gly Gly Trp Ala His Tyr Val Gly Gln Glu Lys Leu Arg Pro 585 590 595 atg aat ggt tgg gca cag tat gcc ttt gct aca gac tgg cag cgt cca 1939 Met Asn Gly Trp Ala Gln Tyr Ala Phe Ala Thr Asp Trp Gln Arg Pro 600 605 610 cca cgt cag atg atc acc act ggt ttc tac tac ctc acc acg gat cag 1987 Pro Arg Gln Met Ile Thr Thr Gly Phe Tyr Tyr Leu Thr Thr Asp Gln 615 620 625 tgg agg tat gac aac act cgt gct aat cgt ctg gct tcc cca ctg gct 2035 Trp Arg Tyr Asp Asn Thr Arg Ala Asn Arg Leu Ala Ser Pro Leu Ala 630 635 640 645 aat cgt ggc acc gtg ggt gac aaa atg acg gcg gat acc ttg gtg gaa 2083 Asn Arg Gly Thr Val Gly Asp Lys Met Thr Ala Asp Thr Leu Val Glu 650 655 660 tcc atg aaa cgt gga tgg atg ccg tca ttc ccg caa ttc aac cgc aat 2131 Ser Met Lys Arg Gly Trp Met Pro Ser Phe Pro Gln Phe Asn Arg Asn 665 670 675 ccc ctc atc ttg agc cag gag gcg gaa gaa aag ggc gtg tct gtt tct 2179 Pro Leu Ile Leu Ser Gln Glu Ala Glu Glu Lys Gly Val Ser Val Ser 680 685 690 gac cat att gtt cag cag ctc acc gat ggt gac ttg cag ttc gcc tgc 2227 Asp His Ile Val Gln Gln Leu Thr Asp Gly Asp Leu Gln Phe Ala Cys 695 700 705 gag gat ccg gat gca ccg gaa aac tgg cca cgc att ctg ctt aac tgg 2275 Glu Asp Pro Asp Ala Pro Glu Asn Trp Pro Arg Ile Leu Leu Asn Trp 710 715 720 725 cgc aca aac cta atg ggc tct tca gct aag ggc acg gag ttt ttc ttg 2323 Arg Thr Asn Leu Met Gly Ser Ser Ala Lys Gly Thr Glu Phe Phe Leu 730 735 740 cgc cat atg ttg ggt gtg gat tct gat gca tct gct gaa gaa aac gcg 2371 Arg His Met Leu Gly Val Asp Ser Asp Ala Ser Ala Glu Glu Asn Ala 745 750 755 ccg gag gat cgt cca agt tcc att gtg tgg agg gat gag gct ccg gaa 2419 Pro Glu Asp Arg Pro Ser Ser Ile Val Trp Arg Asp Glu Ala Pro Glu 760 765 770 gga aag ctc gat ttg atg ctg acc acg gat ttc cgc aac act tcc acc 2467 Gly Lys Leu Asp Leu Met Leu Thr Thr Asp Phe Arg Asn Thr Ser Thr 775 780 785 acc ttg gtc tcg gat atc gtg ctg ccg gca gcc acc tgg tat gag aag 2515 Thr Leu Val Ser Asp Ile Val Leu Pro Ala Ala Thr Trp Tyr Glu Lys 790 795 800 805 cat gat ttg tcc acc acg gat atg cac ccc ttc atc cac tcg ttc aat 2563 His Asp Leu Ser Thr Thr Asp Met His Pro Phe Ile His Ser Phe Asn 810 815 820 gct gcg atc aac cca ccg tgg gag acg cgt act gac tgg gag gtc ttc 2611 Ala Ala Ile Asn Pro Pro Trp Glu Thr Arg Thr Asp Trp Glu Val Phe 825 830 835 cac gat ctc acc aaa gaa ttc tcc tca cag gca gca acc tgg ttg ggc 2659 His Asp Leu Thr Lys Glu Phe Ser Ser Gln Ala Ala Thr Trp Leu Gly 840 845 850 acc caa acc gat gtg atc acc gca ccg att gcc cat gac tcc ccg gat 2707 Thr Gln Thr Asp Val Ile Thr Ala Pro Ile Ala His Asp Ser Pro Asp 855 860 865 gag ctc aat atg cct ggc ggt atc gtg cca gat att gat gag gtc ggg 2755 Glu Leu Asn Met Pro Gly Gly Ile Val Pro Asp Ile Asp Glu Val Gly 870 875 880 885 ctg atc cct ggc aag acg atg gcc aag atc atc ccg gtg gaa cgt gat 2803 Leu Ile Pro Gly Lys Thr Met Ala Lys Ile Ile Pro Val Glu Arg Asp 890 895 900 tac tcc aag gtg tat gaa aag tgg aca cac ttg gga cca ctc acc gcc 2851 Tyr Ser Lys Val Tyr Glu Lys Trp Thr His Leu Gly Pro Leu Thr Ala 905 910 915 aaa gcg ggt acc gga acc cac ggc act gcg ttt aac gtg acc aag caa 2899 Lys Ala Gly Thr Gly Thr His Gly Thr Ala Phe Asn Val Thr Lys Gln 920 925 930 acc gag gag ctg gcg ctg atc aac ggc acc tcc atc acg gag tct gct 2947 Thr Glu Glu Leu Ala Leu Ile Asn Gly Thr Ser Ile Thr Glu Ser Ala 935 940 945 ggt gaa cgc ccc cga ctt gat tcc gca gtc aag gcc att gag atg att 2995 Gly Glu Arg Pro Arg Leu Asp Ser Ala Val Lys Ala Ile Glu Met Ile 950 955 960 965 ctg cac tta agt ggc gtg tct aat ggt gag gtt gcc gcc gaa ggt ttc 3043 Leu His Leu Ser Gly Val Ser Asn Gly Glu Val Ala Ala Glu Gly Phe 970 975 980 aag aat cag ggt gca cgc gtg ggc acc gac atg atg gat ctg att gca 3091 Lys Asn Gln Gly Ala Arg Val Gly Thr Asp Met Met Asp Leu Ile Ala 985 990 995 gac cac gaa ggt acc cac atc aat tgg gac atg gtc aaa gaa cgt ccc 3139 Asp His Glu Gly Thr His Ile Asn Trp Asp Met Val Lys Glu Arg Pro 1000 1005 1010 gcc gag gtg atc acc tca ccg gag tgg act ggt tcc aag aag gac gga 3187 Ala Glu Val Ile Thr Ser Pro Glu Trp Thr Gly Ser Lys Lys Asp Gly 1015 1020 1025 cgt cgc tac acc gcg ttt tcc atc aac att gaa tac gac aag ccg tgg 3235 Arg Arg Tyr Thr Ala Phe Ser Ile Asn Ile Glu Tyr Asp Lys Pro Trp 1030 1035 1040 1045 cac acc ctg tct ggt cgc atg cac tac tac ctc gac cac gat tgg ttt 3283 His Thr Leu Ser Gly Arg Met His Tyr Tyr Leu Asp His Asp Trp Phe 1050 1055 1060 att gat tac ggc gag cag ttg cca atc ttt agg cca ccg ttg gac aag 3331 Ile Asp Tyr Gly Glu Gln Leu Pro Ile Phe Arg Pro Pro Leu Asp Lys 1065 1070 1075 atc cac atc aat ggt gag gtc ggc cct ggc cag tcg gtc aca ggc acc 3379 Ile His Ile Asn Gly Glu Val Gly Pro Gly Gln Ser Val Thr Gly Thr 1080 1085 1090 gac ggc gaa cca gaa gta acc gtg cgt tat ctg acc acc cac aac aag 3427 Asp Gly Glu Pro Glu Val Thr Val Arg Tyr Leu Thr Thr His Asn Lys 1095 1100 1105 tgg tcg att cac tcg cag tac tac gac aat ctg cat gtg ctt tct att 3475 Trp Ser Ile His Ser Gln Tyr Tyr Asp Asn Leu His Val Leu Ser Ile 1110 1115 1120 1125 tct cgt ggc ggc cag gtg atc tgg atg tcc aac aag gat gca gag aaa 3523 Ser Arg Gly Gly Gln Val Ile Trp Met Ser Asn Lys Asp Ala Glu Lys 1130 1135 1140 ctc ggt atc gct gac aac gat tgg atc gag gct tat aac cgc aac ggc 3571 Leu Gly Ile Ala Asp Asn Asp Trp Ile Glu Ala Tyr Asn Arg Asn Gly 1145 1150 1155 gtt gtt tct gct cgt gcg att gtc tcc cac cgc att cct gaa ggc acc 3619 Val Val Ser Ala Arg Ala Ile Val Ser His Arg Ile Pro Glu Gly Thr 1160 1165 1170 gtg ttt atg aac cac gcg cag gaa cgc acc gct ggc acc ccg ctg aac 3667 Val Phe Met Asn His Ala Gln Glu Arg Thr Ala Gly Thr Pro Leu Asn 1175 1180 1185 gag aag tct ggc agg cgc ggc gga act cac aac tct ctt act cga atc 3715 Glu Lys Ser Gly Arg Arg Gly Gly Thr His Asn Ser Leu Thr Arg Ile 1190 1195 1200 1205 atg att aag ccg gtc cat gtt gcc ggt ggc tac ggc cac tta acc tat 3763 Met Ile Lys Pro Val His Val Ala Gly Gly Tyr Gly His Leu Thr Tyr 1210 1215 1220 ggc ttc aac tac atc ggc cca acc gga aat aac cgc gat gag gtc acc 3811 Gly Phe Asn Tyr Ile Gly Pro Thr Gly Asn Asn Arg Asp Glu Val Thr 1225 1230 1235 aga att cgt cgc cgc tcc cag gag gtg cag tac taatgaaggt catggctcag 3864 Arg Ile Arg Arg Arg Ser Gln Glu Val Gln Tyr 1240 1245 atcgcaatga 3874 <210> 60 <211> 1248 <212> PRT <213> Corynebacterium glutamicum <400> 60 Met Thr Thr Thr Thr Ser Ser Gly Lys Ser Ser Glu Lys Ile Asn Pro 1 5 10 15 Leu Phe Lys Leu Gly Ser Phe Leu Arg Lys Gly Thr Val Gly Ser Glu 20 25 30 Gly Gln Gln Ile Phe Leu Gln Gly Gly Arg Gln Ala Asp Val Phe Tyr 35 40 45 Arg Asn Arg Trp Ala Phe Asp Lys Val Val Arg Ser Thr His Gly Val 50 55 60 Asn Cys Thr Gly Ser Cys Ser Trp Lys Val Tyr Val Lys Asp Gly Val 65 70 75 80 Ile Thr Trp Glu Ser Gln Ala Val Asp Tyr Pro Thr Thr Gly Ala Asp 85 90 95 Met Pro Asp Tyr Glu Pro Arg Gly Cys Pro Arg Gly Ala Ser Phe Ser 100 105 110 Trp Tyr Thr Tyr Ser Pro Thr Arg Ile Arg Tyr Pro Tyr Ile Arg Gly 115 120 125 Val Leu Val Asp Met Tyr Arg Glu Ala Lys Glu Arg Leu Gly Asp Pro 130 135 140 Val Leu Ala Trp Arg Asp Ile Val Glu Thr Pro Glu Lys Arg Lys Ala 145 150 155 160 Tyr Val Ser Gln Arg Gly Lys Gly Gly Leu Ile Arg Val Gln Tyr Glu 165 170 175 Glu Ala Met Glu Ile Ala Ala Ala Ala His Val Tyr Thr Ile Arg Gln 180 185 190 Tyr Gly Pro Asp Arg Ile His Gly Phe Thr Val Ile Pro Ala Met Ser 195 200 205 Gln Val Ser Tyr Gly Ala Gly Thr Arg Phe Leu Gln Met Ile Gly Gly 210 215 220 Val Ala Leu Ser Phe Tyr Asp Trp Tyr Ala Asp Leu Pro Pro Ala Ser 225 230 235 240 Pro Gln Thr Phe Gly Asp Gln Thr Asp Val Pro Glu Ser Gly Asp Trp 245 250 255 Tyr Asn Ser Ser Tyr Leu Met Met Trp Gly Ser Asn Ile Pro Val Thr 260 265 270 Arg Thr Pro Asp Ser His Phe Met Val Glu Ala Arg Tyr Lys Gly Thr 275 280 285 Lys Val Val Val Val Pro Pro Asp Phe Ala Asp Ser Thr Lys Phe Ala 290 295 300 Asp Glu Trp Ala Arg Ile His Pro Gly Thr Asp Gly Ala Leu Ala Phe 305 310 315 320 Ala Met Gly His Val Ile Leu Lys Glu Phe His Val Asp Lys Lys Thr 325 330 335 Pro Tyr Phe Met Asp Tyr Met Arg Lys Tyr Thr Asp Ser Pro Phe Leu 340 345 350 Val Glu Leu Asp Glu His Gly Asp Gly Thr Tyr Thr Pro Gly Lys Phe 355 360 365 Leu Thr Ala Asp Arg Ala Ala Asp Ile Ser Pro Ala Leu Ala Ala Thr 370 375 380 Pro Asn Ala Thr His Arg Leu Leu Val Leu Gln Lys Asp Gly Ser Val 385 390 395 400 Val Asp Pro Gly Gly Thr Val Ala Asp Arg Trp Gly Glu Glu Gly Met 405 410 415 Gly Lys Trp Asn Leu Arg Leu Asp Gly Val Asp Pro Val Met Thr Ile 420 425 430 Ala Asp Val Gln Thr Asp Thr Glu Thr Ala Glu Val Leu Phe Pro Arg 435 440 445 Phe Asp Leu Pro Ala Thr Ala Thr Gln Glu Gly Pro Ile Gly Ala Gly 450 455 460 Thr Ile Ser Arg Gly Val Pro Thr Ile Thr Leu Asn Gly Arg Lys Tyr 465 470 475 480 Thr Thr Val Phe Asp Val Leu Leu Ala His Tyr Gly Val Asn Arg Glu 485 490 495 Glu Leu Asn Leu Pro Gly Glu Trp Pro Lys Asp Phe Gln Asp Pro Val 500 505 510 Met Gly Thr Pro Ala Trp Gln Glu Glu Leu Thr Gly Val Pro Ala Asn 515 520 525 Gln Ala Ile Arg Leu Gly Arg Glu Phe Ala Gln Asn Ala Asp Asp Ser 530 535 540 Lys Gly Arg Ser Gln Ile Ile Met Gly Ala Gly Val Asn His Tyr Phe 545 550 555 560 His Ala Asp Ser Ile Tyr Arg Thr Phe Leu Ala Leu Thr Ser Met Cys 565 570 575 Gly Thr Gln Gly Val Asn Gly Gly Gly Trp Ala His Tyr Val Gly Gln 580 585 590 Glu Lys Leu Arg Pro Met Asn Gly Trp Ala Gln Tyr Ala Phe Ala Thr 595 600 605 Asp Trp Gln Arg Pro Pro Arg Gln Met Ile Thr Thr Gly Phe Tyr Tyr 610 615 620 Leu Thr Thr Asp Gln Trp Arg Tyr Asp Asn Thr Arg Ala Asn Arg Leu 625 630 635 640 Ala Ser Pro Leu Ala Asn Arg Gly Thr Val Gly Asp Lys Met Thr Ala 645 650 655 Asp Thr Leu Val Glu Ser Met Lys Arg Gly Trp Met Pro Ser Phe Pro 660 665 670 Gln Phe Asn Arg Asn Pro Leu Ile Leu Ser Gln Glu Ala Glu Glu Lys 675 680 685 Gly Val Ser Val Ser Asp His Ile Val Gln Gln Leu Thr Asp Gly Asp 690 695 700 Leu Gln Phe Ala Cys Glu Asp Pro Asp Ala Pro Glu Asn Trp Pro Arg 705 710 715 720 Ile Leu Leu Asn Trp Arg Thr Asn Leu Met Gly Ser Ser Ala Lys Gly 725 730 735 Thr Glu Phe Phe Leu Arg His Met Leu Gly Val Asp Ser Asp Ala Ser 740 745 750 Ala Glu Glu Asn Ala Pro Glu Asp Arg Pro Ser Ser Ile Val Trp Arg 755 760 765 Asp Glu Ala Pro Glu Gly Lys Leu Asp Leu Met Leu Thr Thr Asp Phe 770 775 780 Arg Asn Thr Ser Thr Thr Leu Val Ser Asp Ile Val Leu Pro Ala Ala 785 790 795 800 Thr Trp Tyr Glu Lys His Asp Leu Ser Thr Thr Asp Met His Pro Phe 805 810 815 Ile His Ser Phe Asn Ala Ala Ile Asn Pro Pro Trp Glu Thr Arg Thr 820 825 830 Asp Trp Glu Val Phe His Asp Leu Thr Lys Glu Phe Ser Ser Gln Ala 835 840 845 Ala Thr Trp Leu Gly Thr Gln Thr Asp Val Ile Thr Ala Pro Ile Ala 850 855 860 His Asp Ser Pro Asp Glu Leu Asn Met Pro Gly Gly Ile Val Pro Asp 865 870 875 880 Ile Asp Glu Val Gly Leu Ile Pro Gly Lys Thr Met Ala Lys Ile Ile 885 890 895 Pro Val Glu Arg Asp Tyr Ser Lys Val Tyr Glu Lys Trp Thr His Leu 900 905 910 Gly Pro Leu Thr Ala Lys Ala Gly Thr Gly Thr His Gly Thr Ala Phe 915 920 925 Asn Val Thr Lys Gln Thr Glu Glu Leu Ala Leu Ile Asn Gly Thr Ser 930 935 940 Ile Thr Glu Ser Ala Gly Glu Arg Pro Arg Leu Asp Ser Ala Val Lys 945 950 955 960 Ala Ile Glu Met Ile Leu His Leu Ser Gly Val Ser Asn Gly Glu Val 965 970 975 Ala Ala Glu Gly Phe Lys Asn Gln Gly Ala Arg Val Gly Thr Asp Met 980 985 990 Met Asp Leu Ile Ala Asp His Glu Gly Thr His Ile Asn Trp Asp Met 995 1000 1005 Val Lys Glu Arg Pro Ala Glu Val Ile Thr Ser Pro Glu Trp Thr Gly 1010 1015 1020 Ser Lys Lys Asp Gly Arg Arg Tyr Thr Ala Phe Ser Ile Asn Ile Glu 1025 1030 1035 1040 Tyr Asp Lys Pro Trp His Thr Leu Ser Gly Arg Met His Tyr Tyr Leu 1045 1050 1055 Asp His Asp Trp Phe Ile Asp Tyr Gly Glu Gln Leu Pro Ile Phe Arg 1060 1065 1070 Pro Pro Leu Asp Lys Ile His Ile Asn Gly Glu Val Gly Pro Gly Gln 1075 1080 1085 Ser Val Thr Gly Thr Asp Gly Glu Pro Glu Val Thr Val Arg Tyr Leu 1090 1095 1100 Thr Thr His Asn Lys Trp Ser Ile His Ser Gln Tyr Tyr Asp Asn Leu 1105 1110 1115 1120 His Val Leu Ser Ile Ser Arg Gly Gly Gln Val Ile Trp Met Ser Asn 1125 1130 1135 Lys Asp Ala Glu Lys Leu Gly Ile Ala Asp Asn Asp Trp Ile Glu Ala 1140 1145 1150 Tyr Asn Arg Asn Gly Val Val Ser Ala Arg Ala Ile Val Ser His Arg 1155 1160 1165 Ile Pro Glu Gly Thr Val Phe Met Asn His Ala Gln Glu Arg Thr Ala 1170 1175 1180 Gly Thr Pro Leu Asn Glu Lys Ser Gly Arg Arg Gly Gly Thr His Asn 1185 1190 1195 1200 Ser Leu Thr Arg Ile Met Ile Lys Pro Val His Val Ala Gly Gly Tyr 1205 1210 1215 Gly His Leu Thr Tyr Gly Phe Asn Tyr Ile Gly Pro Thr Gly Asn Asn 1220 1225 1230 Arg Asp Glu Val Thr Arg Ile Arg Arg Arg Ser Gln Glu Val Gln Tyr 1235 1240 1245 <210> 61 <211> 2011 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1981) <223> RXA01385 <400> 61 atcacacttt cattcgcagt gtgatctgaa ctacatttct ggttactgta cggaacacgc 60 tccgtgaatg agataggaaa tcccctcgaa aggaccagac atg cag ttt cat tat 115 Met Gln Phe His Tyr 1 5 gaa gga tac gca acc ggt gac cca atg gag atg cgc gcg gaa ggt agc 163 Glu Gly Tyr Ala Thr Gly Asp Pro Met Glu Met Arg Ala Glu Gly Ser 10 15 20 gga atc aac cgc ccg gac gat ctc ccc gag gtc atg gat gtt ctc atc 211 Gly Ile Asn Arg Pro Asp Asp Leu Pro Glu Val Met Asp Val Leu Ile 25 30 35 gtt ggt gca ggt ccg gct ggc acc atc gca gcg gct cag ctt tcc cga 259 Val Gly Ala Gly Pro Ala Gly Thr Ile Ala Ala Ala Gln Leu Ser Arg 40 45 50 ttc ccc aat gtg acc acc cgc ctc gta gag aga agc gac cgt cgc ctc 307 Phe Pro Asn Val Thr Thr Arg Leu Val Glu Arg Ser Asp Arg Arg Leu 55 60 65 gaa cta gcc aat gca gat ggc gtg cac tcc cga acc att gaa act ttc 355 Glu Leu Ala Asn Ala Asp Gly Val His Ser Arg Thr Ile Glu Thr Phe 70 75 80 85 cag gca ttt ggt ttc gcc cac gag atc ctc gcc gaa gct cat gaa atc 403 Gln Ala Phe Gly Phe Ala His Glu Ile Leu Ala Glu Ala His Glu Ile 90 95 100 acc gac atg gcg ttc tgg aag ccg gac ccg caa aac cct cgt gag atc 451 Thr Asp Met Ala Phe Trp Lys Pro Asp Pro Gln Asn Pro Arg Glu Ile 105 110 115 att cgc gac aac agc acc cgc gag ctg cca cag cac atc agt gaa ttt 499 Ile Arg Asp Asn Ser Thr Arg Glu Leu Pro Gln His Ile Ser Glu Phe 120 125 130 ccg atg gcg ttg ctc acc cag acc cgc atc atc gac cac ttc aac cgg 547 Pro Met Ala Leu Leu Thr Gln Thr Arg Ile Ile Asp His Phe Asn Arg 135 140 145 ttc atg aag aac tcc cca acc agg atg aag cct gac tat gga tac gag 595 Phe Met Lys Asn Ser Pro Thr Arg Met Lys Pro Asp Tyr Gly Tyr Glu 150 155 160 165 ttc gtg gac ttt gaa gta gaa gaa gac gca gaa tat ccg gta att gtc 643 Phe Val Asp Phe Glu Val Glu Glu Asp Ala Glu Tyr Pro Val Ile Val 170 175 180 acc ctc cgc cgc acc agt ggc gag caa act ggc gaa ttg gtc acc gtc 691 Thr Leu Arg Arg Thr Ser Gly Glu Gln Thr Gly Glu Leu Val Thr Val 185 190 195 cgc acc aag tac ctg gtc ggt gcc gat ggt gca cga agc caa gtg cgc 739 Arg Thr Lys Tyr Leu Val Gly Ala Asp Gly Ala Arg Ser Gln Val Arg 200 205 210 aaa tca ctg gga tac cga ctc caa ggt aag cag gct aac cac gct tgg 787 Lys Ser Leu Gly Tyr Arg Leu Gln Gly Lys Gln Ala Asn His Ala Trp 215 220 225 ggt gtg atg gat att cac gcg aac acc gag ttc ccc gac gtg cgc aag 835 Gly Val Met Asp Ile His Ala Asn Thr Glu Phe Pro Asp Val Arg Lys 230 235 240 245 aag tgc acc atc aaa tct gat tcg ggt cgc acc atc ttg ctc atc cca 883 Lys Cys Thr Ile Lys Ser Asp Ser Gly Arg Thr Ile Leu Leu Ile Pro 250 255 260 cgt gag ggt ggc ttc ctc ttc cgt ctc tac gtt gac ctg ggc gaa gta 931 Arg Glu Gly Gly Phe Leu Phe Arg Leu Tyr Val Asp Leu Gly Glu Val 265 270 275 cct gat gat ggc agc aag gct gtt cgt gat acc cca ctc cag gat gtc 979 Pro Asp Asp Gly Ser Lys Ala Val Arg Asp Thr Pro Leu Gln Asp Val 280 285 290 atc gac acc gcg aac cag atc atg gct cca ttc acc ctc gac gtg aaa 1027 Ile Asp Thr Ala Asn Gln Ile Met Ala Pro Phe Thr Leu Asp Val Lys 295 300 305 aac gtt gtg tgg aac tcc atc tac gag gta ggc cac cgc gtc gca gac 1075 Asn Val Val Trp Asn Ser Ile Tyr Glu Val Gly His Arg Val Ala Asp 310 315 320 325 cat ttc gat gac cgt gtt tca gaa aaa acc tcg agc gaa cac cca cgc 1123 His Phe Asp Asp Arg Val Ser Glu Lys Thr Ser Ser Glu His Pro Arg 330 335 340 att ttc att gct ggc gac gcc tgc cac acc cac agc gct aag gct ggc 1171 Ile Phe Ile Ala Gly Asp Ala Cys His Thr His Ser Ala Lys Ala Gly 345 350 355 cag ggc atg aac gtg tcc atg cag gac gga ttc aac ctt ggc tgg aag 1219 Gln Gly Met Asn Val Ser Met Gln Asp Gly Phe Asn Leu Gly Trp Lys 360 365 370 ctt gga cat gtg gcc agc gga aat agc cca cgc gaa cta ctt cag acc 1267 Leu Gly His Val Ala Ser Gly Asn Ser Pro Arg Glu Leu Leu Gln Thr 375 380 385 tac gct gaa gag cgc gaa gac att gcc tac aag ctc atc gag tac gac 1315 Tyr Ala Glu Glu Arg Glu Asp Ile Ala Tyr Lys Leu Ile Glu Tyr Asp 390 395 400 405 aag aac tgg tca aca ctc atg gca aag cca agc agc gaa atg ggc agt 1363 Lys Asn Trp Ser Thr Leu Met Ala Lys Pro Ser Ser Glu Met Gly Ser 410 415 420 gcc caa gac ctt gag gat ttc tac cgc gcg aac tct gag ttc aat gcc 1411 Ala Gln Asp Leu Glu Asp Phe Tyr Arg Ala Asn Ser Glu Phe Asn Ala 425 430 435 ggc tac atg acc cac tat cct cct tct tcc atc aca atg gat ggc agc 1459 Gly Tyr Met Thr His Tyr Pro Pro Ser Ser Ile Thr Met Asp Gly Ser 440 445 450 aac caa gat ctg gca aag ggc tac cca att ggc cga cgc ttc aag tca 1507 Asn Gln Asp Leu Ala Lys Gly Tyr Pro Ile Gly Arg Arg Phe Lys Ser 455 460 465 gcg atg gtt ggt cga gtc tgc gac ttc acc gaa aca cac ctc ggt cac 1555 Ala Met Val Gly Arg Val Cys Asp Phe Thr Glu Thr His Leu Gly His 470 475 480 485 caa gca aca gcc gac gga cgc atg cgc gca tac gtc ttc gca gga tcc 1603 Gln Ala Thr Ala Asp Gly Arg Met Arg Ala Tyr Val Phe Ala Gly Ser 490 495 500 gat gca ctt aac ggc gag ggt tct gag cta gac cgc tgg gca gaa tgg 1651 Asp Ala Leu Asn Gly Glu Gly Ser Glu Leu Asp Arg Trp Ala Glu Trp 505 510 515 gca gag gcg aac ctt gac ccc acg ctt gtc gac gcc aag gtg att tac 1699 Ala Glu Ala Asn Leu Asp Pro Thr Leu Val Asp Ala Lys Val Ile Tyr 520 525 530 caa agc cct tat acc gag ctc gac acc cgc cag gtt cca tcc gtg ttc 1747 Gln Ser Pro Tyr Thr Glu Leu Asp Thr Arg Gln Val Pro Ser Val Phe 535 540 545 aaa cct gca gtc ggg atc ttc gaa ctg acc aat gtg gaa aac tcc ttc 1795 Lys Pro Ala Val Gly Ile Phe Glu Leu Thr Asn Val Glu Asn Ser Phe 550 555 560 565 ggt atc acc acg gac tcc gac atc ttt gat agt cgc gag atc tcc cgc 1843 Gly Ile Thr Thr Asp Ser Asp Ile Phe Asp Ser Arg Glu Ile Ser Arg 570 575 580 gat ggt gtc gtg gtg gta gtc cga cca gac caa tac gtt tcc gga atc 1891 Asp Gly Val Val Val Val Val Arg Pro Asp Gln Tyr Val Ser Gly Ile 585 590 595 ttc cca ctc act gat acc caa ggg ctt ggc gaa ttc ctc acc gga tac 1939 Phe Pro Leu Thr Asp Thr Gln Gly Leu Gly Glu Phe Leu Thr Gly Tyr 600 605 610 ttc ccc aaa atg aaa ggc gca cat cag cta atc aac gcg aac 1981 Phe Pro Lys Met Lys Gly Ala His Gln Leu Ile Asn Ala Asn 615 620 625 taaggcacag ctgttaaaac agtggacgtt 2011 <210> 62 <211> 627 <212> PRT <213> Corynebacterium glutamicum <400> 62 Met Gln Phe His Tyr Glu Gly Tyr Ala Thr Gly Asp Pro Met Glu Met 1 5 10 15 Arg Ala Glu Gly Ser Gly Ile Asn Arg Pro Asp Asp Leu Pro Glu Val 20 25 30 Met Asp Val Leu Ile Val Gly Ala Gly Pro Ala Gly Thr Ile Ala Ala 35 40 45 Ala Gln Leu Ser Arg Phe Pro Asn Val Thr Thr Arg Leu Val Glu Arg 50 55 60 Ser Asp Arg Arg Leu Glu Leu Ala Asn Ala Asp Gly Val His Ser Arg 65 70 75 80 Thr Ile Glu Thr Phe Gln Ala Phe Gly Phe Ala His Glu Ile Leu Ala 85 90 95 Glu Ala His Glu Ile Thr Asp Met Ala Phe Trp Lys Pro Asp Pro Gln 100 105 110 Asn Pro Arg Glu Ile Ile Arg Asp Asn Ser Thr Arg Glu Leu Pro Gln 115 120 125 His Ile Ser Glu Phe Pro Met Ala Leu Leu Thr Gln Thr Arg Ile Ile 130 135 140 Asp His Phe Asn Arg Phe Met Lys Asn Ser Pro Thr Arg Met Lys Pro 145 150 155 160 Asp Tyr Gly Tyr Glu Phe Val Asp Phe Glu Val Glu Glu Asp Ala Glu 165 170 175 Tyr Pro Val Ile Val Thr Leu Arg Arg Thr Ser Gly Glu Gln Thr Gly 180 185 190 Glu Leu Val Thr Val Arg Thr Lys Tyr Leu Val Gly Ala Asp Gly Ala 195 200 205 Arg Ser Gln Val Arg Lys Ser Leu Gly Tyr Arg Leu Gln Gly Lys Gln 210 215 220 Ala Asn His Ala Trp Gly Val Met Asp Ile His Ala Asn Thr Glu Phe 225 230 235 240 Pro Asp Val Arg Lys Lys Cys Thr Ile Lys Ser Asp Ser Gly Arg Thr 245 250 255 Ile Leu Leu Ile Pro Arg Glu Gly Gly Phe Leu Phe Arg Leu Tyr Val 260 265 270 Asp Leu Gly Glu Val Pro Asp Asp Gly Ser Lys Ala Val Arg Asp Thr 275 280 285 Pro Leu Gln Asp Val Ile Asp Thr Ala Asn Gln Ile Met Ala Pro Phe 290 295 300 Thr Leu Asp Val Lys Asn Val Val Trp Asn Ser Ile Tyr Glu Val Gly 305 310 315 320 His Arg Val Ala Asp His Phe Asp Asp Arg Val Ser Glu Lys Thr Ser 325 330 335 Ser Glu His Pro Arg Ile Phe Ile Ala Gly Asp Ala Cys His Thr His 340 345 350 Ser Ala Lys Ala Gly Gln Gly Met Asn Val Ser Met Gln Asp Gly Phe 355 360 365 Asn Leu Gly Trp Lys Leu Gly His Val Ala Ser Gly Asn Ser Pro Arg 370 375 380 Glu Leu Leu Gln Thr Tyr Ala Glu Glu Arg Glu Asp Ile Ala Tyr Lys 385 390 395 400 Leu Ile Glu Tyr Asp Lys Asn Trp Ser Thr Leu Met Ala Lys Pro Ser 405 410 415 Ser Glu Met Gly Ser Ala Gln Asp Leu Glu Asp Phe Tyr Arg Ala Asn 420 425 430 Ser Glu Phe Asn Ala Gly Tyr Met Thr His Tyr Pro Pro Ser Ser Ile 435 440 445 Thr Met Asp Gly Ser Asn Gln Asp Leu Ala Lys Gly Tyr Pro Ile Gly 450 455 460 Arg Arg Phe Lys Ser Ala Met Val Gly Arg Val Cys Asp Phe Thr Glu 465 470 475 480 Thr His Leu Gly His Gln Ala Thr Ala Asp Gly Arg Met Arg Ala Tyr 485 490 495 Val Phe Ala Gly Ser Asp Ala Leu Asn Gly Glu Gly Ser Glu Leu Asp 500 505 510 Arg Trp Ala Glu Trp Ala Glu Ala Asn Leu Asp Pro Thr Leu Val Asp 515 520 525 Ala Lys Val Ile Tyr Gln Ser Pro Tyr Thr Glu Leu Asp Thr Arg Gln 530 535 540 Val Pro Ser Val Phe Lys Pro Ala Val Gly Ile Phe Glu Leu Thr Asn 545 550 555 560 Val Glu Asn Ser Phe Gly Ile Thr Thr Asp Ser Asp Ile Phe Asp Ser 565 570 575 Arg Glu Ile Ser Arg Asp Gly Val Val Val Val Val Arg Pro Asp Gln 580 585 590 Tyr Val Ser Gly Ile Phe Pro Leu Thr Asp Thr Gln Gly Leu Gly Glu 595 600 605 Phe Leu Thr Gly Tyr Phe Pro Lys Met Lys Gly Ala His Gln Leu Ile 610 615 620 Asn Ala Asn 625 <210> 63 <211> 1141 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1111) <223> RXA01386 <400> 63 ttgtgccttt gagattgtgc cgggagcttt agggctcccg gcgttttcct attccgcatt 60 aatgacttat gtgatctaag actctattgt ggtacgcacc atg act gct caa cca 115 Met thr ala gln pro 1 5 gcc cac gag ccg cca agg cat ctc cgg ttc agt acg agc ggg atc ctc 163 Ala His Glu Pro Pro Arg His Leu Arg Phe Ser Thr Ser Gly Ile Leu 10 15 20 ccg gac aat cgc gtc caa atg tgg gag ggc cac aat gct cgc gca ttg 211 Pro Asp Asn Arg Val Gln Met Trp Glu Gly His Asn Ala Arg Ala Leu 25 30 35 ctt ccg ctc gac att aga acc att gac gat cgc ccc atg cag gcc tcc 259 Leu Pro Leu Asp Ile Arg Thr Ile Asp Asp Arg Pro Met Gln Ala Ser 40 45 50 gaa acc aac ctg cac ctc cca tca atg cgg atg gcg agc gta ttc ggg 307 Glu Thr Asn Leu His Leu Pro Ser Met Arg Met Ala Ser Val Phe Gly 55 60 65 act tcg caa ttt gtc gag cgt tca gag agt ttc atc tca gaa aac ccc 355 Thr Ser Gln Phe Val Glu Arg Ser Glu Ser Phe Ile Ser Glu Asn Pro 70 75 80 85 acg ggt gtg gtt gcg atc ttc ttt gcg act gaa ggt gaa gca gtc ttc 403 Thr Gly Val Val Ala Ile Phe Phe Ala Thr Glu Gly Glu Ala Val Phe 90 95 100 ttc cac cgt ggt gga cat gta gcg ctt cgg cca ggt cag gcc att gtt 451 Phe His Arg Gly Gly His Val Ala Leu Arg Pro Gly Gln Ala Ile Val 105 110 115 tac gac gcc gat agg cca ttc ctc cgc gga ttc aac aat cgc ttc cgc 499 Tyr Asp Ala Asp Arg Pro Phe Leu Arg Gly Phe Asn Asn Arg Phe Arg 120 125 130 gag cta gtt ctc acc atc ccg aag cag cgc tac ctt gaa att gtt ggc 547 Glu Leu Val Leu Thr Ile Pro Lys Gln Arg Tyr Leu Glu Ile Val Gly 135 140 145 tca aaa ggc cct gag ctt ccc gct att ttt gag ttc gga gca aca gga 595 Ser Lys Gly Pro Glu Leu Pro Ala Ile Phe Glu Phe Gly Ala Thr Gly 150 155 160 165 acc gcc aat gaa caa gct tta gcg cga cta gtt cag gaa tct cta cac 643 Thr Ala Asn Glu Gln Ala Leu Ala Arg Leu Val Gln Glu Ser Leu His 170 175 180 agg att gaa agt ggc gag ccg aag cat atc gat tcc agt gga cct tta 691 Arg Ile Glu Ser Gly Glu Pro Lys His Ile Asp Ser Ser Gly Pro Leu 185 190 195 gga aaa ccg tgg agc gat atc gag cac gag gcc cac gga ctt atc cgc 739 Gly Lys Pro Trp Ser Asp Ile Glu His Glu Ala His Gly Leu Ile Arg 200 205 210 aat gta ctt ggc gac gcc aca agt agc gaa gaa ggc tta att tct gca 787 Asn Val Leu Gly Asp Ala Thr Ser Ser Glu Glu Gly Leu Ile Ser Ala 215 220 225 gcc cag aga ttt att gac atc aat att tcc gaa agt gac tta caa gcg 835 Ala Gln Arg Phe Ile Asp Ile Asn Ile Ser Glu Ser Asp Leu Gln Ala 230 235 240 245 tcg cgg att gct gca gcc gtg gga atc agc gaa cgc caa cta agt cga 883 Ser Arg Ile Ala Ala Ala Val Gly Ile Ser Glu Arg Gln Leu Ser Arg 250 255 260 atc ttc tca gac tca gga caa act atc gga cgc tac gtc cta aac acc 931 Ile Phe Ser Asp Ser Gly Gln Thr Ile Gly Arg Tyr Val Leu Asn Thr 265 270 275 cga ctg gat ttt gca aag gaa gcg ctg tcg aca ccg gag cga gac aag 979 Arg Leu Asp Phe Ala Lys Glu Ala Leu Ser Thr Pro Glu Arg Asp Lys 280 285 290 gtt tcg gtc agt gag atc ggt aag cgc ttt ggg ttc gct tcc cca agt 1027 Val Ser Val Ser Glu Ile Gly Lys Arg Phe Gly Phe Ala Ser Pro Ser 295 300 305 cat ttc agt cgc acc ttc cgc gag cgg ttt gaa atg acg ccg ctt caa 1075 His Phe Ser Arg Thr Phe Arg Glu Arg Phe Glu Met Thr Pro Leu Gln 310 315 320 325 tgg agg aag gaa tcg cag cgt caa tcc ttt caa gag tgaggttttt 1121 Trp Arg Lys Glu Ser Gln Arg Gln Ser Phe Gln Glu 330 335 gttctcaggc ggacatcatc 1141 <210> 64 <211> 337 <212> PRT <213> Corynebacterium glutamicum <400> 64 Met Thr Ala Gln Pro Ala His Glu Pro Pro Arg His Leu Arg Phe Ser 1 5 10 15 Thr Ser Gly Ile Leu Pro Asp Asn Arg Val Gln Met Trp Glu Gly His 20 25 30 Asn Ala Arg Ala Leu Leu Pro Leu Asp Ile Arg Thr Ile Asp Asp Arg 35 40 45 Pro Met Gln Ala Ser Glu Thr Asn Leu His Leu Pro Ser Met Arg Met 50 55 60 Ala Ser Val Phe Gly Thr Ser Gln Phe Val Glu Arg Ser Glu Ser Phe 65 70 75 80 Ile Ser Glu Asn Pro Thr Gly Val Val Ala Ile Phe Phe Ala Thr Glu 85 90 95 Gly Glu Ala Val Phe Phe His Arg Gly Gly His Val Ala Leu Arg Pro 100 105 110 Gly Gln Ala Ile Val Tyr Asp Ala Asp Arg Pro Phe Leu Arg Gly Phe 115 120 125 Asn Asn Arg Phe Arg Glu Leu Val Leu Thr Ile Pro Lys Gln Arg Tyr 130 135 140 Leu Glu Ile Val Gly Ser Lys Gly Pro Glu Leu Pro Ala Ile Phe Glu 145 150 155 160 Phe Gly Ala Thr Gly Thr Ala Asn Glu Gln Ala Leu Ala Arg Leu Val 165 170 175 Gln Glu Ser Leu His Arg Ile Glu Ser Gly Glu Pro Lys His Ile Asp 180 185 190 Ser Ser Gly Pro Leu Gly Lys Pro Trp Ser Asp Ile Glu His Glu Ala 195 200 205 His Gly Leu Ile Arg Asn Val Leu Gly Asp Ala Thr Ser Ser Glu Glu 210 215 220 Gly Leu Ile Ser Ala Ala Gln Arg Phe Ile Asp Ile Asn Ile Ser Glu 225 230 235 240 Ser Asp Leu Gln Ala Ser Arg Ile Ala Ala Ala Val Gly Ile Ser Glu 245 250 255 Arg Gln Leu Ser Arg Ile Phe Ser Asp Ser Gly Gln Thr Ile Gly Arg 260 265 270 Tyr Val Leu Asn Thr Arg Leu Asp Phe Ala Lys Glu Ala Leu Ser Thr 275 280 285 Pro Glu Arg Asp Lys Val Ser Val Ser Glu Ile Gly Lys Arg Phe Gly 290 295 300 Phe Ala Ser Pro Ser His Phe Ser Arg Thr Phe Arg Glu Arg Phe Glu 305 310 315 320 Met Thr Pro Leu Gln Trp Arg Lys Glu Ser Gln Arg Gln Ser Phe Gln 325 330 335 Glu <210> 65 <211> 1339 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) .. (1309) <223> RXA01430 <400> 65 cgccccaaga acgaaattgt ggaaaaacta gagaagcacc tctagctggt attcttactg 60 cagtcacgtg gatgaactat atccattgat aattttgaac atg aat gat gga agg 115 Met Asn Asp Gly Arg 1 5 agc agg gtg tct aaa gtc ctg aga gtt ggc gat cgc agc ccg cgc gtg 163 Ser Arg Val Ser Lys Val Leu Arg Val Gly Asp Arg Ser Pro Arg Val 10 15 20 gca gaa gtg cgc act acg ctc gct cgc ctc ggt gtg att gaa ggc tat 211 Ala Glu Val Arg Thr Thr Leu Ala Arg Leu Gly Val Ile Glu Gly Tyr 25 30 35 tcc agg gag atg tct gca aag aca gaa tcc cag aag ttc cac gaa gaa 259 Ser Arg Glu Met Ser Ala Lys Thr Glu Ser Gln Lys Phe His Glu Glu 40 45 50 gag acg ctt ttc gac gaa gaa ctc agc ctc agc atc aag tca ttc cag 307 Glu Thr Leu Phe Asp Glu Glu Leu Ser Leu Ser Ile Lys Ser Phe Gln 55 60 65 caa gct cga gga gtc gtt ccc tcc ggg ctt att gac gac ccc acc ctg 355 Gln Ala Arg Gly Val Val Pro Ser Gly Leu Ile Asp Asp Pro Thr Leu 70 75 80 85 cgc gca atc cgc gaa gcc tcc tac acc ctg gga acc cgc gtg ctg gcc 403 Arg Ala Ile Arg Glu Ala Ser Tyr Thr Leu Gly Thr Arg Val Leu Ala 90 95 100 tac cag ccc ggc aac cag ctt gtt ggt gac gac gtt gta gaa atc caa 451 Tyr Gln Pro Gly Asn Gln Leu Val Gly Asp Asp Val Val Glu Ile Gln 105 110 115 tcc cat ctc caa gag ctc ggc ttc tac gcc gac cgt gtg gat gga cat 499 Ser His Leu Gln Glu Leu Gly Phe Tyr Ala Asp Arg Val Asp Gly His 120 125 130 ttt ggc gag ctc aca cac aaa gct gtg atg aac tac caa ctc aac tac 547 Phe Gly Glu Leu Thr His Lys Ala Val Met Asn Tyr Gln Leu Asn Tyr 135 140 145 ggc atg cag gta gac ggc atc tgt ggc cct gac acc atc cgt gcg ctg 595 Gly Met Gln Val Asp Gly Ile Cys Gly Pro Asp Thr Ile Arg Ala Leu 150 155 160 165 tcc cga ctt ggt ctg cgc atc aag ggt ggc tct gct caa gct atc cgt 643 Ser Arg Leu Gly Leu Arg Ile Lys Gly Gly Ser Ala Gln Ala Ile Arg 170 175 180 gaa cgc gaa cgc atg cgc aat gca ggc cca cgt ctt gct ggc aag cgt 691 Glu Arg Glu Arg Met Arg Asn Ala Gly Pro Arg Leu Ala Gly Lys Arg 185 190 195 gtg gtc att gat cct gcg ctt ggg ggc tcc aac aag ggt cag atc gtg 739 Val Val Ile Asp Pro Ala Leu Gly Gly Ser Asn Lys Gly Gln Ile Val 200 205 210 aaa ggc ccc tac ggt gag atc tct gag gaa gaa atc ctc tgg gat ttg 787 Lys Gly Pro Tyr Gly Glu Ile Ser Glu Glu Glu Ile Leu Trp Asp Leu 215 220 225 gcc acc cgc ctg gaa ggt cgc atg atc gca aca ggc atg gaa acc att 835 Ala Thr Arg Leu Glu Gly Arg Met Ile Ala Thr Gly Met Glu Thr Ile 230 235 240 245 ctg tcg cgc ccg cac atg gat gat ccc agc agc cgt gat cgc gcg tcg 883 Leu Ser Arg Pro His Met Asp Asp Pro Ser Ser Arg Asp Arg Ala Ser 250 255 260 atc gcg aat gct ttc ggc gct gac ctc atg ctg agc ctg cac tgc gat 931 Ile Ala Asn Ala Phe Gly Ala Asp Leu Met Leu Ser Leu His Cys Asp 265 270 275 tcc tac ccg aat gaa aaa gct aac ggc gtg gcc agc ttc tac ttc ggt 979 Ser Tyr Pro Asn Glu Lys Ala Asn Gly Val Ala Ser Phe Tyr Phe Gly 280 285 290 tcg gaa aac ggc acc aac tcc ttg acc ggt gaa acg ctc tcc gcg tac 1027 Ser Glu Asn Gly Thr Asn Ser Leu Thr Gly Glu Thr Leu Ser Ala Tyr 295 300 305 atc caa aaa gag atc gtt gcc cgc acc cca ctg aac aac tgt ggc agc 1075 Ile Gln Lys Glu Ile Val Ala Arg Thr Pro Leu Asn Asn Cys Gly Ser 310 315 320 325 cat gcc cgt acc tgg gat ctg ctg cgc ctc acg cgc atg ccc atg gtg 1123 His Ala Arg Thr Trp Asp Leu Leu Arg Leu Thr Arg Met Pro Met Val 330 335 340 gaa gtt gtc acc ggt tac ctc acc aac ccc gat gac ctg gca gtt ctg 1171 Glu Val Val Thr Gly Tyr Leu Thr Asn Pro Asp Asp Leu Ala Val Leu 345 350 355 act gat cca caa atg cgt gat cac att gcc gaa gcc atc gtt gtc gcc 1219 Thr Asp Pro Gln Met Arg Asp His Ile Ala Glu Ala Ile Val Val Ala 360 365 370 gtc aag cgc ctg tac ctc ctt gat gag gaa gca cag ccc aag acc gga 1267 Val Lys Arg Leu Tyr Leu Leu Asp Glu Glu Ala Gln Pro Lys Thr Gly 375 380 385 acc ttc aag ttc tct gag ctg ttg caa tca gag cag gct ggc 1309 Thr Phe Lys Phe Ser Glu Leu Leu Gln Ser Glu Gln Ala Gly 390 395 400 taaatcgcgc ccatctcttg aaggagtcgc 1339 <210> 66 <211> 403 <212> PRT <213> Corynebacterium glutamicum <400> 66 Met Asn Asp Gly Arg Ser Arg Val Ser Lys Val Leu Arg Val Gly Asp 1 5 10 15 Arg Ser Pro Arg Val Ala Glu Val Arg Thr Thr Leu Ala Arg Leu Gly 20 25 30 Val Ile Glu Gly Tyr Ser Arg Glu Met Ser Ala Lys Thr Glu Ser Gln 35 40 45 Lys Phe His Glu Glu Glu Thr Leu Phe Asp Glu Glu Leu Ser Leu Ser 50 55 60 Ile Lys Ser Phe Gln Gln Ala Arg Gly Val Val Pro Ser Gly Leu Ile 65 70 75 80 Asp Asp Pro Thr Leu Arg Ala Ile Arg Glu Ala Ser Tyr Thr Leu Gly 85 90 95 Thr Arg Val Leu Ala Tyr Gln Pro Gly Asn Gln Leu Val Gly Asp Asp 100 105 110 Val Val Glu Ile Gln Ser His Leu Gln Glu Leu Gly Phe Tyr Ala Asp 115 120 125 Arg Val Asp Gly His Phe Gly Glu Leu Thr His Lys Ala Val Met Asn 130 135 140 Tyr Gln Leu Asn Tyr Gly Met Gln Val Asp Gly Ile Cys Gly Pro Asp 145 150 155 160 Thr Ile Arg Ala Leu Ser Arg Leu Gly Leu Arg Ile Lys Gly Gly Ser 165 170 175 Ala Gln Ala Ile Arg Glu Arg Glu Arg Met Arg Asn Ala Gly Pro Arg 180 185 190 Leu Ala Gly Lys Arg Val Val Ile Asp Pro Ala Leu Gly Gly Ser Asn 195 200 205 Lys Gly Gln Ile Val Lys Gly Pro Tyr Gly Glu Ile Ser Glu Glu Glu 210 215 220 Ile Leu Trp Asp Leu Ala Thr Arg Leu Glu Gly Arg Met Ile Ala Thr 225 230 235 240 Gly Met Glu Thr Ile Leu Ser Arg Pro His Met Asp Asp Pro Ser Ser 245 250 255 Arg Asp Arg Ala Ser Ile Ala Asn Ala Phe Gly Ala Asp Leu Met Leu 260 265 270 Ser Leu His Cys Asp Ser Tyr Pro Asn Glu Lys Ala Asn Gly Val Ala 275 280 285 Ser Phe Tyr Phe Gly Ser Glu Asn Gly Thr Asn Ser Leu Thr Gly Glu 290 295 300 Thr Leu Ser Ala Tyr Ile Gln Lys Glu Ile Val Ala Arg Thr Pro Leu 305 310 315 320 Asn Asn Cys Gly Ser His Ala Arg Thr Trp Asp Leu Leu Arg Leu Thr 325 330 335 Arg Met Pro Met Val Glu Val Val Thr Gly Tyr Leu Thr Asn Pro Asp 340 345 350 Asp Leu Ala Val Leu Thr Asp Pro Gln Met Arg Asp His Ile Ala Glu 355 360 365 Ala Ile Val Val Ala Val Lys Arg Leu Tyr Leu Leu Asp Glu Glu Ala 370 375 380 Gln Pro Lys Thr Gly Thr Phe Lys Phe Ser Glu Leu Leu Gln Ser Glu 385 390 395 400 Gln ala gly <210> 67 <211> 2596 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (2566) <223> RXA01435 <400> 67 atgtggtgga caatgttcga cgattggttc ggaccttatc tctggtccta cggtccacca 60 cagctgcagt acatgccaga ggaagaaggg acagaaaacg atg aag gat aat gaa 115 Met Lys Asp Asn Glu 1 5 gat ttc gat cca gat tca cca gca acc gaa gct gtt gcc ttc aac cct 163 Asp Phe Asp Pro Asp Ser Pro Ala Thr Glu Ala Val Ala Phe Asn Pro 10 15 20 ttc gac gat gac gat gag gat gat tcc ccc gct acc tca gcc gtt gcc 211 Phe Asp Asp Asp Asp Glu Asp Asp Ser Pro Ala Thr Ser Ala Val Ala 25 30 35 ttt aac cct ttt gaa gat gac gat gac gac gat gag ttc caa ggc gaa 259 Phe Asn Pro Phe Glu Asp Asp Asp Asp Asp Asp Glu Phe Gln Gly Glu 40 45 50 ggc cta gaa ttc ctg ctg cgc gac ctc gac aat ctg cga gcc acc caa 307 Gly Leu Glu Phe Leu Leu Arg Asp Leu Asp Asn Leu Arg Ala Thr Gln 55 60 65 ggt caa atg gtg gtg gaa caa cca gca gtt gaa gac agc ctc ggg tca 355 Gly Gln Met Val Val Glu Gln Pro Ala Val Glu Asp Ser Leu Gly Ser 70 75 80 85 gca tct gcg cat acg gag aca act gcg gcc tca ctg cgt ccc cgc cca 403 Ala Ser Ala His Thr Glu Thr Thr Ala Ala Ser Leu Arg Pro Arg Pro 90 95 100 gag gtg gat cca agt gag agg agt cgt cga caa gca att tcg ctg ttc 451 Glu Val Asp Pro Ser Glu Arg Ser Arg Arg Gln Ala Ile Ser Leu Phe 105 110 115 cgc gaa cgg cgc cgc gta agg cgc caa tcc cgc cca gtt gct gat ggc 499 Arg Glu Arg Arg Arg Val Arg Arg Gln Ser Arg Pro Val Ala Asp Gly 120 125 130 atg gtg gaa ttg ccg ttc atc acc ccc aaa ccg gaa gat gag ctg ctc 547 Met Val Glu Leu Pro Phe Ile Thr Pro Lys Pro Glu Asp Glu Leu Leu 135 140 145 atc gac ccg gaa aag aag cgc aaa cct ggt gtg gca gcg ccg caa ctt 595 Ile Asp Pro Glu Lys Lys Arg Lys Pro Gly Val Ala Ala Pro Gln Leu 150 155 160 165 gtc gcg ggc gat atc gtc gca gag caa tat gaa gtc ctc ggc gtc atc 643 Val Ala Gly Asp Ile Val Ala Glu Gln Tyr Glu Val Leu Gly Val Ile 170 175 180 gcg cac ggc ggc atg ggt tgg att tac ctc gcc aac gac cgc aat gtg 691 Ala His Gly Gly Met Gly Trp Ile Tyr Leu Ala Asn Asp Arg Asn Val 185 190 195 tcc ggc cgc atc gtg gtg ctc aaa ggc atg atg gcg caa tct tcc gtt 739 Ser Gly Arg Ile Val Val Leu Lys Gly Met Met Ala Gln Ser Ser Val 200 205 210 caa gac caa ggc acc gct gaa gcc gaa cgc gaa ttc ctc gcc gac atc 787 Gln Asp Gln Gly Thr Ala Glu Ala Glu Arg Glu Phe Leu Ala Asp Ile 215 220 225 acc cac ccc ggc atc gtg aag gcc tac aac ttc atc gac gac ccc cgc 835 Thr His Pro Gly Ile Val Lys Ala Tyr Asn Phe Ile Asp Asp Pro Arg 230 235 240 245 gtc ccc ggc gga ttc atc gtc atg gaa tac gtc aac ggc ccc tcc ctg 883 Val Pro Gly Gly Phe Ile Val Met Glu Tyr Val Asn Gly Pro Ser Leu 250 255 260 aaa gac cgc tgc aaa gcc caa ccc gac ggc gtg ctc cgc gtc gac ctc 931 Lys Asp Arg Cys Lys Ala Gln Pro Asp Gly Val Leu Arg Val Asp Leu 265 270 275 gcc atc ggc tac atc ctc gaa ctc ctc ccc gcc atg gac tac ctg cac 979 Ala Ile Gly Tyr Ile Leu Glu Leu Leu Pro Ala Met Asp Tyr Leu His 280 285 290 caa cgc ggc gta gtg tac aac gac ctc aaa ccc gaa aac gtc atc gcc 1027 Gln Arg Gly Val Val Tyr Asn Asp Leu Lys Pro Glu Asn Val Ile Ala 295 300 305 acc gaa gac caa gtt aaa ctc atc gac ctc ggc gcg gtt acc ggc atc 1075 Thr Glu Asp Gln Val Lys Leu Ile Asp Leu Gly Ala Val Thr Gly Ile 310 315 320 325 ggc gca ttc ggc tac att tac ggc acc aaa gga ttc caa gca ccc gaa 1123 Gly Ala Phe Gly Tyr Ile Tyr Gly Thr Lys Gly Phe Gln Ala Pro Glu 330 335 340 gta gcc acc cat ggc ccc tca atc tcc tcc gat att ttc acc atc gga 1171 Val Ala Thr His Gly Pro Ser Ile Ser Ser Asp Ile Phe Thr Ile Gly 345 350 355 cgc acc ctc gca gca ctc acc atg ccc ctc ccc gtt gaa gac ggt gtc 1219 Arg Thr Leu Ala Ala Leu Thr Met Pro Leu Pro Val Glu Asp Gly Val 360 365 370 ctc gca ccg ggc atc ccc tcg ccc aaa aat tca cct ctt ctg cgc agg 1267 Leu Ala Pro Gly Ile Pro Ser Pro Lys Asn Ser Pro Leu Leu Arg Arg 375 380 385 cat ttg tcg ttc tac cgc ctc ctg caa cgc gcc acc gcc gac gac ccc 1315 His Leu Ser Phe Tyr Arg Leu Leu Gln Arg Ala Thr Ala Asp Asp Pro 390 395 400 405 caa cac cga ttc cgc aac gtc agc gaa cta cgc acc caa ctc tac ggc 1363 Gln His Arg Phe Arg Asn Val Ser Glu Leu Arg Thr Gln Leu Tyr Gly 410 415 420 gta ctg cgt gaa att ttg gca gtc cgc gac ggc aaa caa tac ccg cca 1411 Val Leu Arg Glu Ile Leu Ala Val Arg Asp Gly Lys Gln Tyr Pro Pro 425 430 435 cag cac tca cta ttc tcc cca cag cga agc acc ttt ggc acc aaa cac 1459 Gln His Ser Leu Phe Ser Pro Gln Arg Ser Thr Phe Gly Thr Lys His 440 445 450 ctc gtg ttc cgc acc gac cgc atc atc gac ggc atc gaa cga caa gca 1507 Leu Val Phe Arg Thr Asp Arg Ile Ile Asp Gly Ile Glu Arg Gln Ala 455 460 465 cgc atc aca gca cca gaa att gtc tcc gcg ctg cct gtc cca ctc atc 1555 Arg Ile Thr Ala Pro Glu Ile Val Ser Ala Leu Pro Val Pro Leu Ile 470 475 480 485 gac cgc acc gac ccc ggc gcc cgt atg ctc tcc gga tcc tcc tat gca 1603 Asp Arg Thr Asp Pro Gly Ala Arg Met Leu Ser Gly Ser Ser Tyr Ala 490 495 500 gaa ccc tcc gaa acc ctg gaa act ctg cgc aac tcc atg gaa gac gag 1651 Glu Pro Ser Glu Thr Leu Glu Thr Leu Arg Asn Ser Met Glu Asp Glu 505 510 515 caa tac cgc caa tca atc gag atc ccc ctc ggt gtc gtc cga gcc ctc 1699 Gln Tyr Arg Gln Ser Ile Glu Ile Pro Leu Gly Val Val Arg Ala Leu 520 525 530 ctt gac cta ggc ttt acc acc gaa gca cgc caa tgg ctc gaa acc cta 1747 Leu Asp Leu Gly Phe Thr Thr Glu Ala Arg Gln Trp Leu Glu Thr Leu 535 540 545 gag gga cgc atc ggc gac gac tgg cga cac aaa tgg ttc tcc gga atc 1795 Glu Gly Arg Ile Gly Asp Asp Trp Arg His Lys Trp Phe Ser Gly Ile 550 555 560 565 acc tac ctc ctc ctc gac gac tac gcc acc gcc caa gta ttc ttc aac 1843 Thr Tyr Leu Leu Leu Asp Asp Tyr Ala Thr Ala Gln Val Phe Phe Asn 570 575 580 cac gtc ctg acc atc ctg ccc ggc gaa gcc gct cct aaa cta gcc ctc 1891 His Val Leu Thr Ile Leu Pro Gly Glu Ala Ala Pro Lys Leu Ala Leu 585 590 595 gca gct gtt gac gaa ctc atc ctc caa caa atc ggc gcc gaa tcc acc 1939 Ala Ala Val Asp Glu Leu Ile Leu Gln Gln Ile Gly Ala Glu Ser Thr 600 605 610 gcc tat ctc acc cca gac atc gtc tct gca acc gcg acc ctc agc aaa 1987 Ala Tyr Leu Thr Pro Asp Ile Val Ser Ala Thr Ala Thr Leu Ser Lys 615 620 625 gat ttc gaa gac ctc gac gcc tcc gcc ttc gaa tca ctc agc gac acc 2035 Asp Phe Glu Asp Leu Asp Ala Ser Ala Phe Glu Ser Leu Ser Asp Thr 630 635 640 645 tgg tcc cac atc tcc agc gac cca cac gta gtc cgc ttc cat tca ctg 2083 Trp Ser His Ile Ser Ser Asp Pro His Val Val Arg Phe His Ser Leu 650 655 660 cgc ctc tac gca ctt gtc tgg gca acc aac ccc acc acc gtg tcc tcc 2131 Arg Leu Tyr Ala Leu Val Trp Ala Thr Asn Pro Thr Thr Val Ser Ser 665 670 675 gcg ttc ggg ctc gcc cgc caa ctc atg gcc gaa aac caa atc gaa ctc 2179 Ala Phe Gly Leu Ala Arg Gln Leu Met Ala Glu Asn Gln Ile Glu Leu 680 685 690 gca gtc caa gcc cta gac aaa ctc ccc caa tca tcc acc cac tac cga 2227 Ala Val Gln Ala Leu Asp Lys Leu Pro Gln Ser Ser Thr His Tyr Arg 695 700 705 atg gcc acc ctc acc acc atc ttg ttg ctg gtc agc tcc aat ttg agt 2275 Met Ala Thr Leu Thr Thr Ile Leu Leu Leu Val Ser Ser Asn Leu Ser 710 715 720 725 gaa tcc cgc atc cga cgg gct gcc cgc cga ctc acc gaa atc ccc aca 2323 Glu Ser Arg Ile Arg Arg Ala Ala Arg Arg Leu Thr Glu Ile Pro Thr 730 735 740 aac gaa ccc cgc ttc aac caa atc aaa att gcc atc atg tcg gca ggc 2371 Asn Glu Pro Arg Phe Asn Gln Ile Lys Ile Ala Ile Met Ser Ala Gly 745 750 755 ctc agc tgg ctt cga gag cga aaa ctc aaa gct tcc gcc tcc gcg aac 2419 Leu Ser Trp Leu Arg Glu Arg Lys Leu Lys Ala Ser Ala Ser Ala Asn 760 765 770 cct ttg ttt gaa tac ccg ttc tcc caa aaa ggc ctg cgc acc ggc atc 2467 Pro Leu Phe Glu Tyr Pro Phe Ser Gln Lys Gly Leu Arg Thr Gly Ile 775 780 785 tcc gag gca ctc cgc att cag gca cgt tct gca ccg ttc ccg cac cac 2515 Ser Glu Ala Leu Arg Ile Gln Ala Arg Ser Ala Pro Phe Pro His His 790 795 800 805 cgt tac gca ctt gtg gat atg gcg aat gcc gtg cgg cca ctg agt tgg 2563 Arg Tyr Ala Leu Val Asp Met Ala Asn Ala Val Arg Pro Leu Ser Trp 810 815 820 ttc tagctgtttt gacttggggc tatttggtag 2596 Phe <210> 68 <211> 822 <212> PRT <213> Corynebacterium glutamicum <400> 68 Met Lys Asp Asn Glu Asp Phe Asp Pro Asp Ser Pro Ala Thr Glu Ala 1 5 10 15 Val Ala Phe Asn Pro Phe Asp Asp Asp Asp Glu Asp Asp Ser Pro Ala 20 25 30 Thr Ser Ala Val Ala Phe Asn Pro Phe Glu Asp Asp Asp Asp Asp Asp 35 40 45 Glu Phe Gln Gly Glu Gly Leu Glu Phe Leu Leu Arg Asp Leu Asp Asn 50 55 60 Leu Arg Ala Thr Gln Gly Gln Met Val Val Glu Gln Pro Ala Val Glu 65 70 75 80 Asp Ser Leu Gly Ser Ala Ser Ala His Thr Glu Thr Thr Ala Ala Ser 85 90 95 Leu Arg Pro Arg Pro Glu Val Asp Pro Ser Glu Arg Ser Arg Arg Gln 100 105 110 Ala Ile Ser Leu Phe Arg Glu Arg Arg Arg Val Arg Arg Gln Ser Arg 115 120 125 Pro Val Ala Asp Gly Met Val Glu Leu Pro Phe Ile Thr Pro Lys Pro 130 135 140 Glu Asp Glu Leu Leu Ile Asp Pro Glu Lys Lys Arg Lys Pro Gly Val 145 150 155 160 Ala Ala Pro Gln Leu Val Ala Gly Asp Ile Val Ala Glu Gln Tyr Glu 165 170 175 Val Leu Gly Val Ile Ala His Gly Gly Met Gly Trp Ile Tyr Leu Ala 180 185 190 Asn Asp Arg Asn Val Ser Gly Arg Ile Val Val Leu Lys Gly Met Met 195 200 205 Ala Gln Ser Ser Val Gln Asp Gln Gly Thr Ala Glu Ala Glu Arg Glu 210 215 220 Phe Leu Ala Asp Ile Thr His Pro Gly Ile Val Lys Ala Tyr Asn Phe 225 230 235 240 Ile Asp Asp Pro Arg Val Pro Gly Gly Phe Ile Val Met Glu Tyr Val 245 250 255 Asn Gly Pro Ser Leu Lys Asp Arg Cys Lys Ala Gln Pro Asp Gly Val 260 265 270 Leu Arg Val Asp Leu Ala Ile Gly Tyr Ile Leu Glu Leu Leu Pro Ala 275 280 285 Met Asp Tyr Leu His Gln Arg Gly Val Val Tyr Asn Asp Leu Lys Pro 290 295 300 Glu Asn Val Ile Ala Thr Glu Asp Gln Val Lys Leu Ile Asp Leu Gly 305 310 315 320 Ala Val Thr Gly Ile Gly Ala Phe Gly Tyr Ile Tyr Gly Thr Lys Gly 325 330 335 Phe Gln Ala Pro Glu Val Ala Thr His Gly Pro Ser Ile Ser Ser Asp 340 345 350 Ile Phe Thr Ile Gly Arg Thr Leu Ala Ala Leu Thr Met Pro Leu Pro 355 360 365 Val Glu Asp Gly Val Leu Ala Pro Gly Ile Pro Ser Pro Lys Asn Ser 370 375 380 Pro Leu Leu Arg Arg His Leu Ser Phe Tyr Arg Leu Leu Gln Arg Ala 385 390 395 400 Thr Ala Asp Asp Pro Gln His Arg Phe Arg Asn Val Ser Glu Leu Arg 405 410 415 Thr Gln Leu Tyr Gly Val Leu Arg Glu Ile Leu Ala Val Arg Asp Gly 420 425 430 Lys Gln Tyr Pro Pro Gln His Ser Leu Phe Ser Pro Gln Arg Ser Thr 435 440 445 Phe Gly Thr Lys His Leu Val Phe Arg Thr Asp Arg Ile Ile Asp Gly 450 455 460 Ile Glu Arg Gln Ala Arg Ile Thr Ala Pro Glu Ile Val Ser Ala Leu 465 470 475 480 Pro Val Pro Leu Ile Asp Arg Thr Asp Pro Gly Ala Arg Met Leu Ser 485 490 495 Gly Ser Ser Tyr Ala Glu Pro Ser Glu Thr Leu Glu Thr Leu Arg Asn 500 505 510 Ser Met Glu Asp Glu Gln Tyr Arg Gln Ser Ile Glu Ile Pro Leu Gly 515 520 525 Val Val Arg Ala Leu Leu Asp Leu Gly Phe Thr Thr Glu Ala Arg Gln 530 535 540 Trp Leu Glu Thr Leu Glu Gly Arg Ile Gly Asp Asp Trp Arg His Lys 545 550 555 560 Trp Phe Ser Gly Ile Thr Tyr Leu Leu Leu Asp Asp Tyr Ala Thr Ala 565 570 575 Gln Val Phe Phe Asn His Val Leu Thr Ile Leu Pro Gly Glu Ala Ala 580 585 590 Pro Lys Leu Ala Leu Ala Ala Val Asp Glu Leu Ile Leu Gln Gln Ile 595 600 605 Gly Ala Glu Ser Thr Ala Tyr Leu Thr Pro Asp Ile Val Ser Ala Thr 610 615 620 Ala Thr Leu Ser Lys Asp Phe Glu Asp Leu Asp Ala Ser Ala Phe Glu 625 630 635 640 Ser Leu Ser Asp Thr Trp Ser His Ile Ser Ser Asp Pro His Val Val 645 650 655 Arg Phe His Ser Leu Arg Leu Tyr Ala Leu Val Trp Ala Thr Asn Pro 660 665 670 Thr Thr Val Ser Ser Ala Phe Gly Leu Ala Arg Gln Leu Met Ala Glu 675 680 685 Asn Gln Ile Glu Leu Ala Val Gln Ala Leu Asp Lys Leu Pro Gln Ser 690 695 700 Ser Thr His Tyr Arg Met Ala Thr Leu Thr Thr Ile Leu Leu Leu Val 705 710 715 720 Ser Ser Asn Leu Ser Glu Ser Arg Ile Arg Arg Ala Ala Arg Arg Leu 725 730 735 Thr Glu Ile Pro Thr Asn Glu Pro Arg Phe Asn Gln Ile Lys Ile Ala 740 745 750 Ile Met Ser Ala Gly Leu Ser Trp Leu Arg Glu Arg Lys Leu Lys Ala 755 760 765 Ser Ala Ser Ala Asn Pro Leu Phe Glu Tyr Pro Phe Ser Gln Lys Gly 770 775 780 Leu Arg Thr Gly Ile Ser Glu Ala Leu Arg Ile Gln Ala Arg Ser Ala 785 790 795 800 Pro Phe Pro His His Arg Tyr Ala Leu Val Asp Met Ala Asn Ala Val 805 810 815 Arg Pro Leu Ser Trp Phe 820 <210> 69 <211> 1690 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1660) <223> RXA01477 <400> 69 aggtccactt tctaatttga attaagccct cttcaatttt ccgctttcgc tgcgaggtgt 60 gccaatgtgg cgcattattt aatgcatact cggcgctatt ttg agg agc ctc atg 115 Leu Arg Ser Leu Met 1 5 cca cag tta agc aga cgc cag ttc ttg cag aca acc gcc gtt act gca 163 Pro Gln Leu Ser Arg Arg Gln Phe Leu Gln Thr Thr Ala Val Thr Ala 10 15 20 ggt cta gcc act ttt gcg ggc aca cct gca cgc gct gaa gaa cgc caa 211 Gly Leu Ala Thr Phe Ala Gly Thr Pro Ala Arg Ala Glu Glu Arg Gln 25 30 35 ttc cag cat ggc gtg gct tcc ggg gac ccc acc gca acc tct gcc att 259 Phe Gln His Gly Val Ala Ser Gly Asp Pro Thr Ala Thr Ser Ala Ile 40 45 50 ttg tgg act cgg ctg acc cca att ccc gac gcc aca cct gga agt ggc 307 Leu Trp Thr Arg Leu Thr Pro Ile Pro Asp Ala Thr Pro Gly Ser Gly 55 60 65 ctt ggc ccc gac tct cct gtc acc tgg gaa gtc tcc ccc act cct gat 355 Leu Gly Pro Asp Ser Pro Val Thr Trp Glu Val Ser Pro Thr Pro Asp 70 75 80 85 ttc gcc agc atc acg cgc tcg gga acc gta atc acc tcc gca gca agc 403 Phe Ala Ser Ile Thr Arg Ser Gly Thr Val Ile Thr Ser Ala Ala Ser 90 95 100 gat cac acc gtc cac gcg cac gcc acg ggt ttg agc cca tcc acc cgc 451 Asp His Thr Val His Ala His Ala Thr Gly Leu Ser Pro Ser Thr Arg 105 110 115 tat ttc tac cgc ttc atc tcc tcc acc ggc gag gtc tcc cct gtg ggg 499 Tyr Phe Tyr Arg Phe Ile Ser Ser Thr Gly Glu Val Ser Pro Val Gly 120 125 130 cgc acg caa aca aca tcg ctt gtc gac gct ccc ctc ccg cac ctt cgc 547 Arg Thr Gln Thr Thr Ser Le Le Val Asp Ala Pro Leu Pro His Leu Arg 135 140 145 ttt gcc ctt gcg tcg tgt gcc aat tgg gag gca gga ttt ttt gcc gcc 595 Phe Ala Leu Ala Ser Cys Ala Asn Trp Glu Ala Gly Phe Phe Ala Ala 150 155 160 165 tac ggc gac atc gcc cgg cgc gct gac gcc ggc gaa ttg gag atg ttg 643 Tyr Gly Asp Ile Ala Arg Arg Ala Asp Ala Gly Glu Leu Glu Met Leu 170 175 180 att ttt ttg ggt gat tac atc tac gag tac gcc acc ggt atg ttc gcc 691 Ile Phe Leu Gly Asp Tyr Ile Tyr Glu Tyr Ala Thr Gly Met Phe Ala 185 190 195 gga aag gac ggt gtg gtg cgc ccg cat cag cct ctt cat gaa acc atc 739 Gly Lys Asp Gly Val Val Arg Pro His Gln Pro Leu His Glu Thr Ile 200 205 210 acg ttg gag cac tac cgc act cgt tat ggc cat tac cgc agt gac aat 787 Thr Leu Glu His Tyr Arg Thr Arg Tyr Gly His Tyr Arg Ser Asp Asn 215 220 225 cac ttg cag gca gcg cat gcg gcg ttg ccg tgg att gtc atg tgg gat 835 His Leu Gln Ala Ala His Ala Ala Leu Pro Trp Ile Val Met Trp Asp 230 235 240 245 gac cat gag tcg gcc aac aac tct aat cgt gag ggc gcg cag aat cat 883 Asp His Glu Ser Ala Asn Asn Ser Asn Arg Glu Gly Ala Gln Asn His 250 255 260 tcc gct gat gag ggt tcg tgg gtg gat cgt caa aat gct gct cgg cag 931 Ser Ala Asp Glu Gly Ser Trp Val Asp Arg Gln Asn Ala Ala Arg Gln 265 270 275 gtc ttt ttg gag tgg atg ccg atc cgc cag gag gac acg ctc tat cgt 979 Val Phe Leu Glu Trp Met Pro Ile Arg Gln Glu Asp Thr Leu Tyr Arg 280 285 290 tcc ttc act ttt ggt gac ctc gcc acg ctg tca ctt ctt gat ctt cga 1027 Ser Phe Thr Phe Gly Asp Leu Ala Thr Leu Ser Leu Leu Asp Leu Arg 295 300 305 agt ttc aga gat cca gca ccc tcc cag caa cag tgg ctg gag ggt caa 1075 Ser Phe Arg Asp Pro Ala Pro Ser Gln Gln Gln Trp Leu Glu Gly Gln 310 315 320 325 cgt gcg gac acc atg atg ggg tcg cag cag ttt gag tgg ctg aaa tcc 1123 Arg Ala Asp Thr Met Met Gly Ser Gln Gln Phe Glu Trp Leu Lys Ser 330 335 340 aac gtg gaa cac acc acc acg acg tgg aat atc atc ggc agc tca gtg 1171 Asn Val Glu His Thr Thr Thr Thr Thr Trp Asn Ile Ile Gly Ser Ser Val 345 350 355 atg ttt gcc ccc atg gca att acc ggg cag cct ctt ttc cag atc cct 1219 Met Phe Ala Pro Met Ala Ile Thr Gly Gln Pro Leu Phe Gln Ile Pro 360 365 370 gaa cct att ccc gcc aat ttg gat cag tgg gac ggc tac tcc cgt gag 1267 Glu Pro Ile Pro Ala Asn Leu Asp Gln Trp Asp Gly Tyr Ser Arg Glu 375 380 385 cgc gac cga ctc cta gct gta ctt gcc gat ttc gcc act cca acg ctt 1315 Arg Asp Arg Leu Leu Ala Val Leu Ala Asp Phe Ala Thr Pro Thr Leu 390 395 400 405 ttt cta tct ggc gat atc cac tcc gaa tgg gca aac gcc atc cgg ttt 1363 Phe Leu Ser Gly Asp Ile His Ser Glu Trp Ala Asn Ala Ile Arg Phe 410 415 420 aat ggt cga gaa atc ggt gtc gag gca gta tgc agc tcc atc acc tca 1411 Asn Gly Arg Glu Ile Gly Val Glu Ala Val Cys Ser Ser Ile Thr Ser 425 430 435 gct aat gtc aac gac ttc gcc aaa ctc cct gag gac aat ccg gtc tcc 1459 Ala Asn Val Asn Asp Phe Ala Lys Leu Pro Glu Asp Asn Pro Val Ser 440 445 450 ctg caa gcg gaa caa gta atc cga gcc aac agt tcg cat gtg cgc cac 1507 Leu Gln Ala Glu Gln Val Ile Arg Ala Asn Ser Ser His Val Arg His 455 460 465 gtt gat ctt gac gcc cac ggc tac gcc acg gtg aat ctc acc caa gat 1555 Val Asp Leu Asp Ala His Gly Tyr Ala Thr Val Asn Leu Thr Gln Asp 470 475 480 485 ggc gcg cac atg gtc tgg cac cgc gtc gcc gat ctc tcc ctt ccg gac 1603 Gly Ala His Met Val Trp His Arg Val Ala Asp Leu Ser Leu Pro Asp 490 495 500 tca gaa gtt gct ccg gca att gca ctt gag tgg aaa cca ggc gtc gga 1651 Ser Glu Val Ala Pro Ala Ile Ala Leu Glu Trp Lys Pro Gly Val Gly 505 510 515 ttc act act tgagctgctg atttgtaggt tttaagacct 1690 Phe Thr Thr 520 <210> 70 <211> 520 <212> PRT <213> Corynebacterium glutamicum <400> 70 Leu Arg Ser Leu Met Pro Gln Leu Ser Arg Arg Gln Phe Leu Gln Thr 1 5 10 15 Thr Ala Val Thr Ala Gly Leu Ala Thr Phe Ala Gly Thr Pro Ala Arg 20 25 30 Ala Glu Glu Arg Gln Phe Gln His Gly Val Ala Ser Gly Asp Pro Thr 35 40 45 Ala Thr Ser Ala Ile Leu Trp Thr Arg Leu Thr Pro Ile Pro Asp Ala 50 55 60 Thr Pro Gly Ser Gly Leu Gly Pro Asp Ser Pro Val Thr Trp Glu Val 65 70 75 80 Ser Pro Thr Pro Asp Phe Ala Ser Ile Thr Arg Ser Gly Thr Val Ile 85 90 95 Thr Ser Ala Ala Ser Asp His Thr Val His Ala His Ala Thr Gly Leu 100 105 110 Ser Pro Ser Thr Arg Tyr Phe Tyr Arg Phe Ile Ser Ser Thr Gly Glu 115 120 125 Val Ser Pro Val Gly Arg Thr Gln Thr Thr Ser Leu Val Asp Ala Pro 130 135 140 Leu Pro His Leu Arg Phe Ala Leu Ala Ser Cys Ala Asn Trp Glu Ala 145 150 155 160 Gly Phe Phe Ala Ala Tyr Gly Asp Ile Ala Arg Arg Ala Asp Ala Gly 165 170 175 Glu Leu Glu Met Leu Ile Phe Leu Gly Asp Tyr Ile Tyr Glu Tyr Ala 180 185 190 Thr Gly Met Phe Ala Gly Lys Asp Gly Val Val Arg Pro His Gln Pro 195 200 205 Leu His Glu Thr Ile Thr Leu Glu His Tyr Arg Thr Arg Tyr Gly His 210 215 220 Tyr Arg Ser Asp Asn His Leu Gln Ala Ala His Ala Ala Leu Pro Trp 225 230 235 240 Ile Val Met Trp Asp Asp His Glu Ser Ala Asn Asn Ser Asn Arg Glu 245 250 255 Gly Ala Gln Asn His Ser Ala Asp Glu Gly Ser Trp Val Asp Arg Gln 260 265 270 Asn Ala Ala Arg Gln Val Phe Leu Glu Trp Met Pro Ile Arg Gln Glu 275 280 285 Asp Thr Leu Tyr Arg Ser Phe Thr Phe Gly Asp Leu Ala Thr Leu Ser 290 295 300 Leu Leu Asp Leu Arg Ser Phe Arg Asp Pro Ala Pro Ser Gln Gln Gln 305 310 315 320 Trp Leu Glu Gly Gln Arg Ala Asp Thr Met Met Gly Ser Gln Gln Phe 325 330 335 Glu Trp Leu Lys Ser Asn Val Glu His Thr Thr Thr Thr Thr Trp Asn Ile 340 345 350 Ile Gly Ser Ser Val Met Phe Ala Pro Met Ala Ile Thr Gly Gln Pro 355 360 365 Leu Phe Gln Ile Pro Glu Pro Ile Pro Ala Asn Leu Asp Gln Trp Asp 370 375 380 Gly Tyr Ser Arg Glu Arg Asp Arg Leu Leu Ala Val Leu Ala Asp Phe 385 390 395 400 Ala Thr Pro Thr Leu Phe Leu Ser Gly Asp Ile His Ser Glu Trp Ala 405 410 415 Asn Ala Ile Arg Phe Asn Gly Arg Glu Ile Gly Val Glu Ala Val Cys 420 425 430 Ser Ser Ile Thr Ser Ala Asn Val Asn Asp Phe Ala Lys Leu Pro Glu 435 440 445 Asp Asn Pro Val Ser Leu Gln Ala Glu Gln Val Ile Arg Ala Asn Ser 450 455 460 Ser His Val Arg His Val Asp Leu Asp Ala His Gly Tyr Ala Thr Val 465 470 475 480 Asn Leu Thr Gln Asp Gly Ala His Met Val Trp His Arg Val Ala Asp 485 490 495 Leu Ser Leu Pro Asp Ser Glu Val Ala Pro Ala Ile Ala Leu Glu Trp 500 505 510 Lys Pro Gly Val Gly Phe Thr Thr 515 520 <210> 71 <211> 760 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (730) <223> RXA01607 <400> 71 gggctgaagg gctgggcgga acaataatta ttgaatctac aatcggatcg ggaactggaa 60 tttccgcccg ttttccctat ccacaaaagg accaagataa gtg atc cgt att ctg 115 Val Ile Arg Ile Leu 1 5 ttg gct gat gat cat ccc gtt gtt cgc gca ggc ctt gcc tcc ttg ctg 163 Leu Ala Asp Asp His Pro Val Val Arg Ala Gly Leu Ala Ser Leu Leu 10 15 20 gtg agt gaa gat gat ttt gag ata gtg gac atg gtg ggc acc cca gat 211 Val Ser Glu Asp Asp Phe Glu Ile Val Asp Met Val Gly Thr Pro Asp 25 30 35 gat gcc gtt gcg cgc gcc gcg gaa ggc ggg gtg gat gtg gtg ttg atg 259 Asp Ala Val Ala Arg Ala Ala Glu Gly Gly Val Asp Val Val Leu Met 40 45 50 gat ctg cgt ttt ggt gat caa cca ggc atc gag gtc gcc ggc ggg gta 307 Asp Leu Arg Phe Gly Asp Gln Pro Gly Ile Glu Val Ala Gly Gly Val 55 60 65 gag gca acg cgt cgc atc cgt gcg ctg gac aac ccg cca cag gta ctg 355 Glu Ala Thr Arg Arg Ile Arg Ala Leu Asp Asn Pro Pro Gln Val Leu 70 75 80 85 gtg gtg acc aac tac tcc aca gac ggc gat gtg gtg ggc gca gta tct 403 Val Val Thr Asn Tyr Ser Thr Asp Gly Asp Val Val Gly Ala Val Ser 90 95 100 gct ggt gcc gtg ggg tat ttg ctc aaa gat agc tcc cca gaa gat ctc 451 Ala Gly Ala Val Gly Tyr Leu Leu Lys Asp Ser Ser Pro Glu Asp Leu 105 110 115 att gcc ggt gtt cgc gat gcc gcg cgg gga gaa tca gtg ctt tca aag 499 Ile Ala Gly Val Arg Asp Ala Ala Arg Gly Glu Ser Val Leu Ser Lys 120 125 130 cag gtc gcc agc aag atc atg ggg cgg atg aac aac ccc atg act gct 547 Gln Val Ala Ser Lys Ile Met Gly Arg Met Asn Asn Pro Met Thr Ala 135 140 145 ctc agt gcc aga gaa att gaa gtg ctg tcc ttg gtg gcg caa ggg caa 595 Leu Ser Ala Arg Glu Ile Glu Val Leu Ser Leu Val Ala Gln Gly Gln 150 155 160 165 agc aat aga gaa atc ggc aag aaa ctt ttc ctc act gag gcc acg gtg 643 Ser Asn Arg Glu Ile Gly Lys Lys Leu Phe Leu Thr Glu Ala Thr Val 170 175 180 aaa agt cac atg ggg cat gtg ttc aac aag ctg gat gtc acc tct aga 691 Lys Ser His Met Gly His Val Phe Asn Lys Leu Asp Val Thr Ser Arg 185 190 195 aca gct gcg gta gct gaa gcc aga cag cgc gga att atc tagacgcaca 740 Thr Ala Ala Val Ala Glu Ala Arg Gln Arg Gly Ile Ile 200 205 210 cgtgttggta accgatcaca 760 <210> 72 <211> 210 <212> PRT <213> Corynebacterium glutamicum <400> 72 Val Ile Arg Ile Leu Leu Ala Asp Asp His Pro Val Val Arg Ala Gly 1 5 10 15 Leu Ala Ser Leu Leu Val Ser Glu Asp Asp Phe Glu Ile Val Asp Met 20 25 30 Val Gly Thr Pro Asp Asp Ala Val Ala Arg Ala Ala Glu Gly Gly Val 35 40 45 Asp Val Val Leu Met Asp Leu Arg Phe Gly Asp Gln Pro Gly Ile Glu 50 55 60 Val Ala Gly Gly Val Glu Ala Thr Arg Arg Ile Arg Ala Leu Asp Asn 65 70 75 80 Pro Pro Gln Val Leu Val Val Thr Asn Tyr Ser Thr Asp Gly Asp Val 85 90 95 Val Gly Ala Val Ser Ala Gly Ala Val Gly Tyr Leu Leu Lys Asp Ser 100 105 110 Ser Pro Glu Asp Leu Ile Ala Gly Val Arg Asp Ala Ala Arg Gly Glu 115 120 125 Ser Val Leu Ser Lys Gln Val Ala Ser Lys Ile Met Gly Arg Met Asn 130 135 140 Asn Pro Met Thr Ala Leu Ser Ala Arg Glu Ile Glu Val Leu Ser Leu 145 150 155 160 Val Ala Gln Gly Gln Ser Asn Arg Glu Ile Gly Lys Lys Leu Phe Leu 165 170 175 Thr Glu Ala Thr Val Lys Ser His Met Gly His Val Phe Asn Lys Leu 180 185 190 Asp Val Thr Ser Arg Thr Ala Ala Val Ala Glu Ala Arg Gln Arg Gly 195 200 205 Ile Ile 210 <210> 73 <211> 1381 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1351) <223> RXA01642 <400> 73 cttgaaaggt tggaatcact gtgtcttgca gctagtctgc cactcgcact gcatttgctg 60 gatgtatttg gaatgctgcg cggtcttgat attggattcg gtg gat aag atg cgc 115 Val Asp Lys Met Arg 1 5 aga ctc atc gcg gtt agc ttg gcc gct ctg ttt atg ttg gct tcc act 163 Arg Leu Ile Ala Val Ser Leu Ala Ala Leu Phe Met Leu Ala Ser Thr 10 15 20 cca gcg acg agg gca cag gaa gta gaa gct ctc gct tgc ccc gag gta 211 Pro Ala Thr Arg Ala Gln Glu Val Glu Ala Leu Ala Cys Pro Glu Val 25 30 35 gcg atc gcc gat cct tcc tcc gca gtt tta gat gaa cac ctt tcg cag 259 Ala Ile Ala Asp Pro Ser Ser Ala Val Leu Asp Glu His Leu Ser Gln 40 45 50 tca tta tcc caa gct cac caa cta gca act ggc gcc ggt gtg atg gtg 307 Ser Leu Ser Gln Ala His Gln Leu Ala Thr Gly Ala Gly Val Met Val 55 60 65 gca gtc atc gac acc gga gta tcc ctg cat cca cgt ctg ccc cac tta 355 Ala Val Ile Asp Thr Gly Val Ser Leu His Pro Arg Leu Pro His Leu 70 75 80 85 att ccc ggc ggt gat ttc gtg ggc gcc cac caa agc ccc gat gtg cca 403 Ile Pro Gly Gly Asp Phe Val Gly Ala His Gln Ser Pro Asp Val Pro 90 95 100 ggt gaa ctt atc gat tgc gac ggc cac ggc acc atc gtc gcc gga atc 451 Gly Glu Leu Ile Asp Cys Asp Gly His Gly Thr Ile Val Ala Gly Ile 105 110 115 atc gcc tcc caa gga aac ccc ggc acc ggc tgg cca tat gac ggc agc 499 Ile Ala Ser Gln Gly Asn Pro Gly Thr Gly Trp Pro Tyr Asp Gly Ser 120 125 130 tcc gat cct tat atc ggt gtc gcc cca gat tcc gga atc atc tcc att 547 Ser Asp Pro Tyr Ile Gly Val Ala Pro Asp Ser Gly Ile Ile Ser Ile 135 140 145 aaa caa acc agc tca tat gtg cgt act cgt gaa gat tcc aac gtc gga 595 Lys Gln Thr Ser Ser Tyr Val Arg Thr Arg Glu Asp Ser Asn Val Gly 150 155 160 165 acg ctg agc acc ctg gcg gaa tcc atc cac cga gct ctc gat tcc ggt 643 Thr Leu Ser Thr Leu Ala Glu Ser Ile His Arg Ala Leu Asp Ser Gly 170 175 180 gcc cac gtg atc aat att tcc gtg gtg tcc tgt ttg ccc caa tca ccc 691 Ala His Val Ile Asn Ile Ser Val Val Ser Cys Leu Pro Gln Ser Pro 185 190 195 gac gag gcc gca tcg ttc cag cct ctg acg gat gct ctt aac aga gca 739 Asp Glu Ala Ala Ser Phe Gln Pro Leu Thr Asp Ala Leu Asn Arg Ala 200 205 210 gaa ctt caa ggg gtg ata gtg gtg gca gca gca gga aac ctc ggg cag 787 Glu Leu Gln Gly Val Ile Val Val Ala Ala Ala Gly Asn Leu Gly Gln 215 220 225 gat tgt cca gtt gga tct acc gtt tat cct gca cat tca gac act gtg 835 Asp Cys Pro Val Gly Ser Thr Val Tyr Pro Ala His Ser Asp Thr Val 230 235 240 245 ctc tct gtg tcg gca cgt ttt gat tct cac acg ctt gca gaa tat tcc 883 Leu Ser Val Ser Ala Arg Phe Asp Ser His Thr Leu Ala Glu Tyr Ser 250 255 260 atg cct ggc aac caa caa atc ctc tct gca cca agc cac att cag gct 931 Met Pro Gly Asn Gln Gln Ile Leu Ser Ala Pro Ser His Ile Gln Ala 265 270 275 ggt cta tca ccg cgt ggc gac ggc ttc gcc agc cac atg atc acc acc 979 Gly Leu Ser Pro Arg Gly Asp Gly Phe Ala Ser His Met Ile Thr Thr 280 285 290 gct ggc gaa agc ccc ttc gag ggc acc agt ttt gcc gct cca gtt gtc 1027 Ala Gly Glu Ser Pro Phe Glu Gly Thr Ser Phe Ala Ala Pro Val Val 295 300 305 agc gcc aca gct gca ctg ctt cgc cag cat ttt ccc ttt gcc aca ccc 1075 Ser Ala Thr Ala Ala Leu Leu Arg Gln His Phe Pro Phe Ala Thr Pro 310 315 320 325 tat gaa att cgt gca cga atc ttc aac agc atc gac cct gca aga ggc 1123 Tyr Glu Ile Arg Ala Arg Ile Phe Asn Ser Ile Asp Pro Ala Arg Gly 330 335 340 gct att gat ccc tac ctg gca ctt act caa gaa atc tat ccc acc act 1171 Ala Ile Asp Pro Tyr Leu Ala Leu Thr Gln Glu Ile Tyr Pro Thr Thr 345 350 355 ccc ctg gtt cat gag atc gca cta agt gtt ccc acg ccg ccg gat gat 1219 Pro Leu Val His Glu Ile Ala Leu Ser Val Pro Thr Pro Pro Asp Asp 360 365 370 tct cca cgg gag cgg ggc atc cta gtt acc gca atc att gtt ggg ttg 1267 Ser Pro Arg Glu Arg Gly Ile Leu Val Thr Ala Ile Val Gly Leu 375 380 385 ctc gca gtg tta gct gtg ctg atg gga cta cgc cga att cat cat cac 1315 Leu Ala Val Leu Ala Val Leu Met Gly Leu Arg Arg Ile His His His 390 395 400 405 tcg gcc ttt caa aaa gct agc tca agt gtt atc act taatctatga 1361 Ser Ala Phe Gln Lys Ala Ser Ser Ser Val Ile Thr 410 415 ggcaccgttc agaatcccat 1381 <210> 74 <211> 417 <212> PRT <213> Corynebacterium glutamicum <400> 74 Val Asp Lys Met Arg Arg Leu Ile Ala Val Ser Leu Ala Ala Leu Phe 1 5 10 15 Met Leu Ala Ser Thr Pro Ala Thr Arg Ala Gln Glu Val Glu Ala Leu 20 25 30 Ala Cys Pro Glu Val Ala Ile Ala Asp Pro Ser Ser Ala Val Leu Asp 35 40 45 Glu His Leu Ser Gln Ser Leu Ser Gln Ala His Gln Leu Ala Thr Gly 50 55 60 Ala Gly Val Met Val Ala Val Ile Asp Thr Gly Val Ser Leu His Pro 65 70 75 80 Arg Leu Pro His Leu Ile Pro Gly Gly Asp Phe Val Gly Ala His Gln 85 90 95 Ser Pro Asp Val Pro Gly Glu Leu Ile Asp Cys Asp Gly His Gly Thr 100 105 110 Ile Val Ala Gly Ile Ile Ala Ser Gln Gly Asn Pro Gly Thr Gly Trp 115 120 125 Pro Tyr Asp Gly Ser Ser Asp Pro Tyr Ile Gly Val Ala Pro Asp Ser 130 135 140 Gly Ile Ile Ser Ile Lys Gln Thr Ser Ser Tyr Val Arg Thr Arg Glu 145 150 155 160 Asp Ser Asn Val Gly Thr Leu Ser Thr Leu Ala Glu Ser Ile His Arg 165 170 175 Ala Leu Asp Ser Gly Ala His Val Ile Asn Ile Ser Val Val Ser Cys 180 185 190 Leu Pro Gln Ser Pro Asp Glu Ala Ala Ser Phe Gln Pro Leu Thr Asp 195 200 205 Ala Leu Asn Arg Ala Glu Leu Gln Gly Val Ile Val Val Ala Ala Ala 210 215 220 Gly Asn Leu Gly Gln Asp Cys Pro Val Gly Ser Thr Val Tyr Pro Ala 225 230 235 240 His Ser Asp Thr Val Leu Ser Val Ser Ala Arg Phe Asp Ser His Thr 245 250 255 Leu Ala Glu Tyr Ser Met Pro Gly Asn Gln Gln Ile Leu Ser Ala Pro 260 265 270 Ser His Ile Gln Ala Gly Leu Ser Pro Arg Gly Asp Gly Phe Ala Ser 275 280 285 His Met Ile Thr Thr Ala Gly Glu Ser Pro Phe Glu Gly Thr Ser Phe 290 295 300 Ala Ala Pro Val Val Ser Ala Thr Ala Ala Leu Leu Arg Gln His Phe 305 310 315 320 Pro Phe Ala Thr Pro Tyr Glu Ile Arg Ala Arg Ile Phe Asn Ser Ile 325 330 335 Asp Pro Ala Arg Gly Ala Ile Asp Pro Tyr Leu Ala Leu Thr Gln Glu 340 345 350 Ile Tyr Pro Thr Thr Pro Leu Val His Glu Ile Ala Leu Ser Val Pro 355 360 365 Thr Pro Pro Asp Asp Ser Pro Arg Glu Arg Gly Ile Leu Val Thr Ala 370 375 380 Ile Ile Val Gly Leu Leu Ala Val Leu Ala Val Leu Met Gly Leu Arg 385 390 395 400 Arg Ile His His His Ser Ala Phe Gln Lys Ala Ser Ser Ser Val Ile 405 410 415 Thr <210> 75 <211> 1618 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1588) <223> RXA01653 <400> 75 caacagggtg ggggagtcgt cgaggttttc attcagggtg aatgctctcc ttgtttcaga 60 tgttcaacgc tccataaagt agaccgcaat ctagacaaag atg tct att tta att 115 Met Ser Ile Leu Ile 1 5 aag gag cag aac atg gcc acg gcc gag aac aca aca cag gag aat cgg 163 Lys Glu Gln Asn Met Ala Thr Ala Glu Asn Thr Thr Gln Glu Asn Arg 10 15 20 aaa atc ctg ttc aac gca ttt gat atg aac tgc gtt gcg cat cag tcc 211 Lys Ile Leu Phe Asn Ala Phe Asp Met Asn Cys Val Ala His Gln Ser 25 30 35 cca gga ctg tgg aca cac ccg aag gat aag gcg cga gac tac aac act 259 Pro Gly Leu Trp Thr His Pro Lys Asp Lys Ala Arg Asp Tyr Asn Thr 40 45 50 ctt gat tac tgg gtg cac ctt gcc aag act ttg gag aag ggc ctt ttc 307 Leu Asp Tyr Trp Val His Leu Ala Lys Thr Leu Glu Lys Gly Leu Phe 55 60 65 gac ggc ctt ttc atc gca gat gtg ctt gga act tac gat gtt tat ggt 355 Asp Gly Leu Phe Ile Ala Asp Val Leu Gly Thr Tyr Asp Val Tyr Gly 70 75 80 85 tct agt aat gaa gcg gcg ttg agc agt ggt gcg cag gtg cct gtc aat 403 Ser Ser Asn Glu Ala Ala Leu Ser Ser Gly Ala Gln Val Pro Val Asn 90 95 100 gat ccg atc ctt ctt gtt tct gcg atg gcc tat gcc aca aag aac ctc 451 Asp Pro Ile Leu Leu Val Ser Ala Met Ala Tyr Ala Thr Lys Asn Leu 105 110 115 ggg ttt ggc att act gca ggt act gcc tat gag cac ccg tat cct ttt 499 Gly Phe Gly Ile Thr Ala Gly Thr Ala Tyr Glu His Pro Tyr Pro Phe 120 125 130 gcg cgg cgt ctg gcc aca ctt gat cac ctg act aat ggg cgt gtg ggg 547 Ala Arg Arg Leu Ala Thr Leu Asp His Leu Thr Asn Gly Arg Val Gly 135 140 145 tgg aat gtg gtt act ggc tat ctt ccc tct gct gct caa aac atg ggt 595 Trp Asn Val Val Thr Gly Tyr Leu Pro Ser Ala Ala Gln Asn Met Gly 150 155 160 165 gac acc gat cag ctg cca cat gat gag cgc tat gac aaa gca gat gaa 643 Asp Thr Asp Gln Leu Pro His Asp Glu Arg Tyr Asp Lys Ala Asp Glu 170 175 180 tac ctg gaa gtg atc tac aag ctt ctc gag ggc tcc tgg gaa gac gat 691 Tyr Leu Glu Val Ile Tyr Lys Leu Leu Glu Gly Ser Trp Glu Asp Asp 185 190 195 gct gtt caa aac aat acg gag acg agt gtc ttt acg gac tcc tcc aaa 739 Ala Val Gln Asn Asn Thr Glu Thr Ser Val Phe Thr Asp Ser Ser Lys 200 205 210 gtg cac gcc att aat cat cat ggc aag tac ttt gat gtg ccg ggc att 787 Val His Ala Ile Asn His His Gly Lys Tyr Phe Asp Val Pro Gly Ile 215 220 225 gcc atc act gag ccg agt gtg cag cgt acg ccg gtg atc tac cag gcg 835 Ala Ile Thr Glu Pro Ser Val Gln Arg Thr Pro Val Ile Tyr Gln Ala 230 235 240 245 ggt gca tcg ccg cgc gga ttg aaa ttc gct ggt gag aat gca gaa gca 883 Gly Ala Ser Pro Arg Gly Leu Lys Phe Ala Gly Glu Asn Ala Glu Ala 250 255 260 gtg ttt atc aat tcc agc acc gtg gag gca atc acc aag act gtc gca 931 Val Phe Ile Asn Ser Ser Thr Val Glu Ala Ile Thr Lys Thr Val Ala 265 270 275 aaa att cgc gct gct gcg gtc gct gcg gga cgt gat cca cat gcg gtg 979 Lys Ile Arg Ala Ala Ala Val Ala Ala Gly Arg Asp Pro His Ala Val 280 285 290 aag atc ttt gcg atg caa acc atc atc act ggt gaa aca gaa gca gat 1027 Lys Ile Phe Ala Met Gln Thr Ile Ile Thr Gly Glu Thr Glu Ala Asp 295 300 305 gcg cag gca aag ctg gag gaa tac agt cgc tat atc gat cct gtc ggt 1075 Ala Gln Ala Lys Leu Glu Glu Tyr Ser Arg Tyr Ile Asp Pro Val Gly 310 315 320 325 ggt ctg acc ttg atg tct gga tgg acc ggc gcg gat ctg tcg cag tat 1123 Gly Leu Thr Leu Met Ser Gly Trp Thr Gly Ala Asp Leu Ser Gln Tyr 330 335 340 gac ctg gat gaa ccg atc acc aat att gag tca aac gct att cag tcc 1171 Asp Leu Asp Glu Pro Ile Thr Asn Ile Glu Ser Asn Ala Ile Gln Ser 345 350 355 act gca gcc acc att agc aac ggc acc ggt gaa ggt gcg tgg acg gta 1219 Thr Ala Ala Thr Ile Ser Asn Gly Thr Gly Glu Gly Ala Trp Thr Val 360 365 370 cgc aaa ctg ggt gag gca acc ggc atc ggc ggc ttc gga cca gtg ctt 1267 Arg Lys Leu Gly Glu Ala Thr Gly Ile Gly Gly Phe Gly Pro Val Leu 375 380 385 gtg gga tct ggc gct aac gtt gcc gcg gaa ctt gca cgc atc cag gat 1315 Val Gly Ser Gly Ala Asn Val Ala Ala Glu Leu Ala Arg Ile Gln Asp 390 395 400 405 ctc agc gat gtt gat ggt ttc aac ctt gct tat gcc atc acc cca gga 1363 Leu Ser Asp Val Asp Gly Phe Asn Leu Ala Tyr Ala Ile Thr Pro Gly 410 415 420 act ttt gaa gat gtc gtg gac ttt gtg gtg cct gag ctg caa aaa ctt 1411 Thr Phe Glu Asp Val Val Asp Phe Val Val Pro Glu Leu Gln Lys Leu 425 430 435 agc cgc tac aag acg gaa tac gcg ccg ggt tcc ttg cgc aac aaa ttg 1459 Ser Arg Tyr Lys Thr Glu Tyr Ala Pro Gly Ser Leu Arg Asn Lys Leu 440 445 450 ctc ggt aaa ggt gat cgc ctg gac gat acc cac cgc ggc gca agc tac 1507 Leu Gly Lys Gly Asp Arg Leu Asp Asp Thr His Arg Gly Ala Ser Tyr 455 460 465 cgc cta ggc gct cgg aac tcc acc gcc act att gat ctc agt tcc ata 1555 Arg Leu Gly Ala Arg Asn Ser Thr Ala Thr Ile Asp Leu Ser Ser Ile 470 475 480 485 tcc gcc caa cta gtt tcc cag gga gcc cac tca tgatctcacc gcaaacaatc 1608 Ser Ala Gln Leu Val Ser Gln Gly Ala His Ser 490 495 atcgacaatc 1618 <210> 76 <211> 496 <212> PRT <213> Corynebacterium glutamicum <400> 76 Met Ser Ile Leu Ile Lys Glu Gln Asn Met Ala Thr Ala Glu Asn Thr 1 5 10 15 Thr Gln Glu Asn Arg Lys Ile Leu Phe Asn Ala Phe Asp Met Asn Cys 20 25 30 Val Ala His Gln Ser Pro Gly Leu Trp Thr His Pro Lys Asp Lys Ala 35 40 45 Arg Asp Tyr Asn Thr Leu Asp Tyr Trp Val His Leu Ala Lys Thr Leu 50 55 60 Glu Lys Gly Leu Phe Asp Gly Leu Phe Ile Ala Asp Val Leu Gly Thr 65 70 75 80 Tyr Asp Val Tyr Gly Ser Ser Asn Glu Ala Ala Leu Ser Ser Gly Ala 85 90 95 Gln Val Pro Val Asn Asp Pro Ile Leu Leu Val Ser Ala Met Ala Tyr 100 105 110 Ala Thr Lys Asn Leu Gly Phe Gly Ile Thr Ala Gly Thr Ala Tyr Glu 115 120 125 His Pro Tyr Pro Phe Ala Arg Arg Leu Ala Thr Leu Asp His Leu Thr 130 135 140 Asn Gly Arg Val Gly Trp Asn Val Val Thr Gly Tyr Leu Pro Ser Ala 145 150 155 160 Ala Gln Asn Met Gly Asp Thr Asp Gln Leu Pro His Asp Glu Arg Tyr 165 170 175 Asp Lys Ala Asp Glu Tyr Leu Glu Val Ile Tyr Lys Leu Leu Glu Gly 180 185 190 Ser Trp Glu Asp Asp Ala Val Gln Asn Asn Thr Glu Thr Ser Val Phe 195 200 205 Thr Asp Ser Ser Lys Val His Ala Ile Asn His His Gly Lys Tyr Phe 210 215 220 Asp Val Pro Gly Ile Ala Ile Thr Glu Pro Ser Val Gln Arg Thr Pro 225 230 235 240 Val Ile Tyr Gln Ala Gly Ala Ser Pro Arg Gly Leu Lys Phe Ala Gly 245 250 255 Glu Asn Ala Glu Ala Val Phe Ile Asn Ser Ser Thr Val Glu Ala Ile 260 265 270 Thr Lys Thr Val Ala Lys Ile Arg Ala Ala Ala Val Ala Ala Gly Arg 275 280 285 Asp Pro His Ala Val Lys Ile Phe Ala Met Gln Thr Ile Ile Thr Gly 290 295 300 Glu Thr Glu Ala Asp Ala Gln Ala Lys Leu Glu Glu Tyr Ser Arg Tyr 305 310 315 320 Ile Asp Pro Val Gly Gly Leu Thr Leu Met Ser Gly Trp Thr Gly Ala 325 330 335 Asp Leu Ser Gln Tyr Asp Leu Asp Glu Pro Ile Thr Asn Ile Glu Ser 340 345 350 Asn Ala Ile Gln Ser Thr Ala Ala Thr Ile Ser Asn Gly Thr Gly Glu 355 360 365 Gly Ala Trp Thr Val Arg Lys Leu Gly Glu Ala Thr Gly Ile Gly Gly 370 375 380 Phe Gly Pro Val Leu Val Gly Ser Gly Ala Asn Val Ala Ala Glu Leu 385 390 395 400 Ala Arg Ile Gln Asp Leu Ser Asp Val Asp Gly Phe Asn Leu Ala Tyr 405 410 415 Ala Ile Thr Pro Gly Thr Phe Glu Asp Val Val Asp Phe Val Val Pro 420 425 430 Glu Leu Gln Lys Leu Ser Arg Tyr Lys Thr Glu Tyr Ala Pro Gly Ser 435 440 445 Leu Arg Asn Lys Leu Leu Gly Lys Gly Asp Arg Leu Asp Asp Thr His 450 455 460 Arg Gly Ala Ser Tyr Arg Leu Gly Ala Arg Asn Ser Thr Ala Thr Ile 465 470 475 480 Asp Leu Ser Ser Ile Ser Ala Gln Leu Val Ser Gln Gly Ala His Ser 485 490 495 <210> 77 <211> 997 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (967) <223> RXA01664 <400> 77 cctcacgagc ggttggcgct aaaatactta gcgtacgcac cccaacgggt taccaaagtg 60 aatggtaggg gaagtttcgg tgtcttatac cggttaggtt ttg ccc gcg ctg cgc 115 Leu Pro Ala Leu Arg 1 5 ttg gtc aca tta acg cct agg ctc ggg gct atg acc gtg ttg att tct 163 Leu Val Thr Leu Thr Pro Arg Leu Gly Ala Met Thr Val Leu Ile Ser 10 15 20 ccg tcc acc ctt gct gaa tca atc cac gct ggt aag aaa caa act gtt 211 Pro Ser Thr Leu Ala Glu Ser Ile His Ala Gly Lys Lys Gln Thr Val 25 30 35 ctc gct gct ttc tgg gct cca att gaa gga gca ggc cgc aca gtt ttc 259 Leu Ala Ala Phe Trp Ala Pro Ile Glu Gly Ala Gly Arg Thr Val Phe 40 45 50 tgc tct gag cac atc cca act tcc att ttc tgc gac cct gcc ctt gag 307 Cys Ser Glu His Ile Pro Thr Ser Ile Phe Cys Asp Pro Ala Leu Glu 55 60 65 ctt tcc gga gtt cct tcc tct gaa gat ggc cgc aac cca ctg cca ccg 355 Leu Ser Gly Val Pro Ser Ser Glu Asp Gly Arg Asn Pro Leu Pro Pro 70 75 80 85 ctg aat gtg ttg gca cgt tct ttc agg acc tgg ggt ttg aat acc gat 403 Leu Asn Val Leu Ala Arg Ser Phe Arg Thr Trp Gly Leu Asn Thr Asp 90 95 100 cgt gaa atc gtg ttt tac gat cag gga cgt ggc ctt ttt gct gca cgc 451 Arg Glu Ile Val Phe Tyr Asp Gln Gly Arg Gly Leu Phe Ala Ala Arg 105 110 115 gcc tgg tgg atc ctc cga tgg gcg ggc atg ccc aac gtt cgc atc ctt 499 Ala Trp Trp Ile Leu Arg Trp Ala Gly Met Pro Asn Val Arg Ile Leu 120 125 130 gac ggt ggt ttc cag aag tgg gaa gac cat gag ctg gga cac gct ggc 547 Asp Gly Gly Phe Gln Lys Trp Glu Asp His Glu Leu Gly His Ala Gly 135 140 145 ggg cct gga aac ttc ccg cac ttt tgc aat gtg cgt ccc aac cca ggt 595 Gly Pro Gly Asn Phe Pro His Phe Cys Asn Val Arg Pro Asn Pro Gly 150 155 160 165 cag ctg tcg gta gcg acc atc gaa gat gtc aag gca cat cag ggc att 643 Gln Leu Ser Val Ala Thr Ile Glu Asp Val Lys Ala His Gln Gly Ile 170 175 180 ttg att gat tct cgc gat gaa caa cga ttt gcg ggt cgc agt gaa aag 691 Leu Ile Asp Ser Arg Asp Glu Gln Arg Phe Ala Gly Arg Ser Glu Lys 185 190 195 ctc gat ctg aaa gcc gga cac att cca ggc gct atc aac atc aac gct 739 Leu Asp Leu Lys Ala Gly His Ile Pro Gly Ala Ile Asn Ile Asn Ala 200 205 210 aaa tct ttg ctg gaa gat gat ttc acc ttc aaa tca cca gaa gaa atc 787 Lys Ser Leu Leu Glu Asp Asp Phe Thr Phe Lys Ser Pro Glu Glu Ile 215 220 225 cgc cag att ttt gcg gac aag ggg gta acc agc gga gag aac gtc atc 835 Arg Gln Ile Phe Ala Asp Lys Gly Val Thr Ser Gly Glu Asn Val Ile 230 235 240 245 gtt tat tcc ggt tcc ggt aac cac tcg tcc cag ttg ctg gct ggc atg 883 Val Tyr Ser Gly Ser Gly Asn His Ser Ser Gln Leu Leu Ala Gly Met 250 255 260 gag cac gcg ggg cta acc ggt gcg agc cat tat ttt gct ggt tgg tca 931 Glu His Ala Gly Leu Thr Gly Ala Ser His Tyr Phe Ala Gly Trp Ser 265 270 275 cag tgg agc gct aac ccc gag aat cct atc gag gcc taaaatcgtg 977 Gln Trp Ser Ala Asn Pro Glu Asn Pro Ile Glu Ala 280 285 gcttgagtac gcactgccag 997 <210> 78 <211> 289 <212> PRT <213> Corynebacterium glutamicum <400> 78 Leu Pro Ala Leu Arg Leu Val Thr Leu Thr Pro Arg Leu Gly Ala Met 1 5 10 15 Thr Val Leu Ile Ser Pro Ser Thr Leu Ala Glu Ser Ile His Ala Gly 20 25 30 Lys Lys Gln Thr Val Leu Ala Ala Phe Trp Ala Pro Ile Glu Gly Ala 35 40 45 Gly Arg Thr Val Phe Cys Ser Glu His Ile Pro Thr Ser Ile Phe Cys 50 55 60 Asp Pro Ala Leu Glu Leu Ser Gly Val Pro Ser Ser Glu Asp Gly Arg 65 70 75 80 Asn Pro Leu Pro Pro Leu Asn Val Leu Ala Arg Ser Phe Arg Thr Trp 85 90 95 Gly Leu Asn Thr Asp Arg Glu Ile Val Phe Tyr Asp Gln Gly Arg Gly 100 105 110 Leu Phe Ala Ala Arg Ala Trp Trp Ile Leu Arg Trp Ala Gly Met Pro 115 120 125 Asn Val Arg Ile Leu Asp Gly Gly Phe Gln Lys Trp Glu Asp His Glu 130 135 140 Leu Gly His Ala Gly Gly Pro Gly Asn Phe Pro His Phe Cys Asn Val 145 150 155 160 Arg Pro Asn Pro Gly Gln Leu Ser Val Ala Thr Ile Glu Asp Val Lys 165 170 175 Ala His Gln Gly Ile Leu Ile Asp Ser Arg Asp Glu Gln Arg Phe Ala 180 185 190 Gly Arg Ser Glu Lys Leu Asp Leu Lys Ala Gly His Ile Pro Gly Ala 195 200 205 Ile Asn Ile Asn Ala Lys Ser Leu Leu Glu Asp Asp Phe Thr Phe Lys 210 215 220 Ser Pro Glu Glu Ile Arg Gln Ile Phe Ala Asp Lys Gly Val Thr Ser 225 230 235 240 Gly Glu Asn Val Ile Val Tyr Ser Gly Ser Gly Asn His Ser Ser Gln 245 250 255 Leu Leu Ala Gly Met Glu His Ala Gly Leu Thr Gly Ala Ser His Tyr 260 265 270 Phe Ala Gly Trp Ser Gln Trp Ser Ala Asn Pro Glu Asn Pro Ile Glu 275 280 285 Ala <210> 79 <211> 1234 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (1204) <223> RXA01728 <400> 79 caagaagtgc gacggctaac agggctggga ttgtcctcaa cttcacttcg ggctccttct 60 tagtaatagg ttcgtagaaa agtttactag cctagagagt atg cga ttt cct gaa 115 Met Arg Phe Pro Glu 1 5 ctc gaa gaa ttg aag aat cgc cgg acc ttg aaa tgg acc cgg ttt cca 163 Leu Glu Glu Leu Lys Asn Arg Arg Thr Leu Lys Trp Thr Arg Phe Pro 10 15 20 gaa gac gtg ctt cct ttg tgg gtt gcg gaa agt gat ttt ggc acc tgc 211 Glu Asp Val Leu Pro Leu Trp Val Ala Glu Ser Asp Phe Gly Thr Cys 25 30 35 ccg cag ttg aag gaa gct atg gca gat gcc gtt gag cgc gag gtc ttc 259 Pro Gln Leu Lys Glu Ala Met Ala Asp Ala Val Glu Arg Glu Val Phe 40 45 50 gga tac cca cca gat gct act ggg ttg aat gat gcg ttg act gga ttc 307 Gly Tyr Pro Pro Asp Ala Thr Gly Leu Asn Asp Ala Leu Thr Gly Phe 55 60 65 tac gag cgt cgc tat ggg ttt ggc cca aat ccg gaa agt gtt ttc gcc 355 Tyr Glu Arg Arg Tyr Gly Phe Gly Pro Asn Pro Glu Ser Val Phe Ala 70 75 80 85 att ccg gat gtg gtt cgt ggc ctg aag ctt gcc att gag cat ttc act 403 Ile Pro Asp Val Val Arg Gly Leu Lys Leu Ala Ile Glu His Phe Thr 90 95 100 aag cct ggt tcg gcg atc att gtg ccg ttg cct gca tac cct cct ttc 451 Lys Pro Gly Ser Ala Ile Ile Val Pro Leu Pro Ala Tyr Pro Pro Phe 105 110 115 att gag ttg cct aag gtg act ggt cgt cag gcg atc tac att gat gcg 499 Ile Glu Leu Pro Lys Val Thr Gly Arg Gln Ala Ile Tyr Ile Asp Ala 120 125 130 cat gag tac gat ttg aag gaa att gag aag gcc ttc gct gac ggt gcg 547 His Glu Tyr Asp Leu Lys Glu Ile Glu Lys Ala Phe Ala Asp Gly Ala 135 140 145 gga tca ctg ttg ttc tgc aat cca cac aac cca ctg ggc acg gtc ttt 595 Gly Ser Leu Leu Phe Cys Asn Pro His Asn Pro Leu Gly Thr Val Phe 150 155 160 165 tct gaa gag tac atc cgc gag ctc acc gat att gcg gcg aag tac gat 643 Ser Glu Glu Tyr Ile Arg Glu Leu Thr Asp Ile Ala Ala Lys Tyr Asp 170 175 180 gcc cgc atc atc gtc gat gag atc cac gcg cca ctg gtt tat gaa ggc 691 Ala Arg Ile Ile Val Asp Glu Ile His Ala Pro Leu Val Tyr Glu Gly 185 190 195 acc cat gtg gtt gct gct ggt gtt tct gag aac gct gca aac act tgc 739 Thr His Val Val Ala Ala Gly Val Ser Glu Asn Ala Ala Asn Thr Cys 200 205 210 atc acc atc acc gca act tct aag gcg tgg aac act gct ggt ttg aag 787 Ile Thr Ile Thr Ala Thr Ser Lys Ala Trp Asn Thr Ala Gly Leu Lys 215 220 225 tgt gct cag atc ttc ttc agt aat gaa gcc gat gtg aag gcc tgg aag 835 Cys Ala Gln Ile Phe Phe Ser Asn Glu Ala Asp Val Lys Ala Trp Lys 230 235 240 245 aat ttg tcg gat att acc cgt gac ggt gtg tcc atc ctt gga ttg atc 883 Asn Leu Ser Asp Ile Thr Arg Asp Gly Val Ser Ile Leu Gly Leu Ile 250 255 260 gct gcg gag aca gtg tac aac gag ggc gaa gaa ttc ctt gat gag tca 931 Ala Ala Glu Thr Val Tyr Asn Glu Gly Glu Glu Phe Leu Asp Glu Ser 265 270 275 att cag att ctc aag gac aac cgt gac ttt gcg gct gct gaa ctg gaa 979 Ile Gln Ile Leu Lys Asp Asn Arg Asp Phe Ala Ala Ala Glu Leu Glu 280 285 290 aag ctt ggc gtg aag gtc tac gca ccg gac tcc act tat ttg atg tgg 1027 Lys Leu Gly Val Lys Val Tyr Ala Pro Asp Ser Thr Tyr Leu Met Trp 295 300 305 ttg gac ttc gct ggc acc aag atc gaa gag gcg cct tct aaa att ctt 1075 Leu Asp Phe Ala Gly Thr Lys Ile Glu Glu Ala Pro Ser Lys Ile Leu 310 315 320 325 cgt gag gag ggt aag gtc atg ctg aat gat ggc gca gct ttt ggt ggt 1123 Arg Glu Glu Gly Lys Val Met Leu Asn Asp Gly Ala Ala Phe Gly Gly 330 335 340 ttc acc acc tgc gct cgt ctt aat ttt gcg tgt tcc aga gag acc ctt 1171 Phe Thr Thr Cys Ala Arg Leu Asn Phe Ala Cys Ser Arg Glu Thr Leu 345 350 355 gag gag ggg ctg cgc cgt atc gcc agc gtg ttg taaataatga gtaaaaagtc 1224 Glu Glu Gly Leu Arg Arg Ile Ala Ser Val Leu 360 365 tgtcctgatt 1234 <210> 80 <211> 368 <212> PRT <213> Corynebacterium glutamicum <400> 80 Met Arg Phe Pro Glu Leu Glu Glu Leu Lys Asn Arg Arg Thr Leu Lys 1 5 10 15 Trp Thr Arg Phe Pro Glu Asp Val Leu Pro Leu Trp Val Ala Glu Ser 20 25 30 Asp Phe Gly Thr Cys Pro Gln Leu Lys Glu Ala Met Ala Asp Ala Val 35 40 45 Glu Arg Glu Val Phe Gly Tyr Pro Pro Asp Ala Thr Gly Leu Asn Asp 50 55 60 Ala Leu Thr Gly Phe Tyr Glu Arg Arg Tyr Gly Phe Gly Pro Asn Pro 65 70 75 80 Glu Ser Val Phe Ala Ile Pro Asp Val Val Arg Gly Leu Lys Leu Ala 85 90 95 Ile Glu His Phe Thr Lys Pro Gly Ser Ala Ile Ile Val Pro Leu Pro 100 105 110 Ala Tyr Pro Pro Phe Ile Glu Leu Pro Lys Val Thr Gly Arg Gln Ala 115 120 125 Ile Tyr Ile Asp Ala His Glu Tyr Asp Leu Lys Glu Ile Glu Lys Ala 130 135 140 Phe Ala Asp Gly Ala Gly Ser Leu Leu Phe Cys Asn Pro His Asn Pro 145 150 155 160 Leu Gly Thr Val Phe Ser Glu Glu Tyr Ile Arg Glu Leu Thr Asp Ile 165 170 175 Ala Ala Lys Tyr Asp Ala Arg Ile Ile Val Asp Glu Ile His Ala Pro 180 185 190 Leu Val Tyr Glu Gly Thr His Val Val Ala Ala Gly Val Ser Glu Asn 195 200 205 Ala Ala Asn Thr Cys Ile Thr Ile Thr Ala Thr Ser Lys Ala Trp Asn 210 215 220 Thr Ala Gly Leu Lys Cys Ala Gln Ile Phe Phe Ser Asn Glu Ala Asp 225 230 235 240 Val Lys Ala Trp Lys Asn Leu Ser Asp Ile Thr Arg Asp Gly Val Ser 245 250 255 Ile Leu Gly Leu Ile Ala Ala Glu Thr Val Tyr Asn Glu Gly Glu Glu 260 265 270 Phe Leu Asp Glu Ser Ile Gln Ile Leu Lys Asp Asn Arg Asp Phe Ala 275 280 285 Ala Ala Glu Leu Glu Lys Leu Gly Val Lys Val Tyr Ala Pro Asp Ser 290 295 300 Thr Tyr Leu Met Trp Leu Asp Phe Ala Gly Thr Lys Ile Glu Glu Ala 305 310 315 320 Pro Ser Lys Ile Leu Arg Glu Glu Gly Lys Val Met Leu Asn Asp Gly 325 330 335 Ala Ala Phe Gly Gly Phe Thr Thr Cys Ala Arg Leu Asn Phe Ala Cys 340 345 350 Ser Arg Glu Thr Leu Glu Glu Gly Leu Arg Arg Ile Ala Ser Val Leu 355 360 365 <210> 81 <211> 1114 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1084) <223> RXA01842 <400> 81 ggcctgcgcg atagatcccc caatgtcggt ggggtccttc gtgatcactt ctttcactct 60 ttgatccacg tcatggtctc gcggtgatca tcttcctacc gtg aca ggc aag gcc 115 Val Thr Gly Lys Ala 1 5 gca aac ggc cgt gac cac agg aaa gaa att cac gag gag agg aag cac 163 Ala Asn Gly Arg Asp His Arg Lys Glu Ile His Glu Glu Arg Lys His 10 15 20 acg atg tcg aag gta tac gtg tcc aac gag tac ggc ggc ccg gaa aac 211 Thr Met Ser Lys Val Tyr Val Ser Asn Glu Tyr Gly Gly Pro Glu Asn 25 30 35 cag gaa ctg atc acc cgc aac acc ccc cag cca ggc ccg gga gaa ctc 259 Gln Glu Leu Ile Thr Arg Asn Thr Pro Gln Pro Gly Pro Gly Glu Leu 40 45 50 ggg gtc aag gtc cac gcg gcc ggg gtc aac ccg ctt gat tgg aag gtc 307 Gly Val Lys Val His Ala Ala Gly Val Asn Pro Leu Asp Trp Lys Val 55 60 65 cgt tcc ggg gtt gcc gga acc ccg cga gag ctt ccg gca ccc ctg ggc 355 Arg Ser Gly Val Ala Gly Thr Pro Arg Glu Leu Pro Ala Pro Leu Gly 70 75 80 85 gag gag gcc tcc ggg atc gtc acc gcc gtt gga gac ggt gtg gag ggc 403 Glu Glu Ala Ser Gly Ile Val Thr Ala Val Gly Asp Gly Val Glu Gly 90 95 100 ttc gcg gcc ggg gat ccg gtg ctc ggc ctg gtg gcc ccc ggc gtc ggc 451 Phe Ala Ala Gly Asp Pro Val Leu Gly Leu Val Ala Pro Gly Val Gly 105 110 115 gga tat gcc gag gac acc ctg ctg gtg gca gag agt acc gtg cta aag 499 Gly Tyr Ala Glu Asp Thr Leu Leu Val Ala Glu Ser Thr Val Leu Lys 120 125 130 ccg gag gag atc tcg ttc acc gac gcc gcc gcg atc ccg gtc gct ggg 547 Pro Glu Glu Ile Ser Phe Thr Asp Ala Ala Ala Ile Pro Val Ala Gly 135 140 145 gcg agc gcc tac gcc ggc act cac cag gtc gag ctt gaa cca ggc cag 595 Ala Ser Ala Tyr Ala Gly Thr His Gln Val Glu Leu Glu Pro Gly Gln 150 155 160 165 tcg ttg ctg atc aat ggg gcc ggt ggt ggg gtc ggg ctg atg gcc gcg 643 Ser Leu Leu Ile Asn Gly Ala Gly Gly Gly Val Gly Leu Met Ala Ala 170 175 180 cag atc gga cgg gtc cac aag ttc cag gtc gtc ggc gtt gac cac gag 691 Gln Ile Gly Arg Val His Lys Phe Gln Val Val Gly Val Asp His Glu 185 190 195 gac aag cgc gag ctc atc gaa tcc acc ggt gct atc ttc gtc gcc acc 739 Asp Lys Arg Glu Leu Ile Glu Ser Thr Gly Ala Ile Phe Val Ala Thr 200 205 210 ggc gac gcc gtc gcg gag cag gtg cgt gcg ctg ctc cct gac ggt gtg 787 Gly Asp Ala Val Ala Glu Gln Val Arg Ala Leu Leu Pro Asp Gly Val 215 220 225 gac gta gtc ttc gac cta gtc ggc ggg gag gcg ttg cgg gtg gtt gct 835 Asp Val Val Phe Asp Leu Val Gly Gly Glu Ala Leu Arg Val Val Ala 230 235 240 245 ccc tta gcg aag aat ccg gcg cac gtg atc tcg gcg gct gat gct gcc 883 Pro Leu Ala Lys Asn Pro Ala His Val Ile Ser Ala Ala Asp Ala Ala 250 255 260 acc gtg gga gaa ctc ggt gga cag gtg ctg cgc cgc acc ccg gaa atg 931 Thr Val Gly Glu Leu Gly Gly Gln Val Leu Arg Arg Thr Pro Glu Met 265 270 275 gtc gga cag atc acc ggg gtg gtc cag tac ggg ctg gtc gac ccg aag 979 Val Gly Gln Ile Thr Gly Val Val Gln Tyr Gly Leu Val Asp Pro Lys 280 285 290 gtc gat acg acc tac ccg ctg gaa cag gcc ggt aag gcc ctg gcc cac 1027 Val Asp Thr Thr Tyr Pro Leu Glu Gln Ala Gly Lys Ala Leu Ala His 295 300 305 gtt gag cag ggc cac gcc cgc ggc aag atc gtc ctc gag ctc atc acc 1075 Val Glu Gln Gly His Ala Arg Gly Lys Ile Val Leu Glu Leu Ile Thr 310 315 320 325 tcc cag gac taaccagaca acgcggtgac ctccctcagg 1114 Ser Gln Asp <210> 82 <211> 328 <212> PRT <213> Corynebacterium glutamicum <400> 82 Val Thr Gly Lys Ala Ala Asn Gly Arg Asp His Arg Lys Glu Ile His 1 5 10 15 Glu Glu Arg Lys His Thr Met Ser Lys Val Tyr Val Ser Asn Glu Tyr 20 25 30 Gly Gly Pro Glu Asn Gln Glu Leu Ile Thr Arg Asn Thr Pro Gln Pro 35 40 45 Gly Pro Gly Glu Leu Gly Val Lys Val His Ala Ala Gly Val Asn Pro 50 55 60 Leu Asp Trp Lys Val Arg Ser Gly Val Ala Gly Thr Pro Arg Glu Leu 65 70 75 80 Pro Ala Pro Leu Gly Glu Glu Ala Ser Gly Ile Val Thr Ala Val Gly 85 90 95 Asp Gly Val Glu Gly Phe Ala Ala Gly Asp Pro Val Leu Gly Leu Val 100 105 110 Ala Pro Gly Val Gly Gly Tyr Ala Glu Asp Thr Leu Leu Val Ala Glu 115 120 125 Ser Thr Val Leu Lys Pro Glu Glu Ile Ser Phe Thr Asp Ala Ala Ala 130 135 140 Ile Pro Val Ala Gly Ala Ser Ala Tyr Ala Gly Thr His Gln Val Glu 145 150 155 160 Leu Glu Pro Gly Gln Ser Leu Leu Ile Asn Gly Ala Gly Gly Gly Val 165 170 175 Gly Leu Met Ala Ala Gln Ile Gly Arg Val His Lys Phe Gln Val Val 180 185 190 Gly Val Asp His Glu Asp Lys Arg Glu Leu Ile Glu Ser Thr Gly Ala 195 200 205 Ile Phe Val Ala Thr Gly Asp Ala Val Ala Glu Gln Val Arg Ala Leu 210 215 220 Leu Pro Asp Gly Val Asp Val Val Phe Asp Leu Val Gly Gly Glu Ala 225 230 235 240 Leu Arg Val Val Ala Pro Leu Ala Lys Asn Pro Ala His Val Ile Ser 245 250 255 Ala Ala Asp Ala Ala Thr Val Gly Glu Leu Gly Gly Gln Val Leu Arg 260 265 270 Arg Thr Pro Glu Met Val Gly Gln Ile Thr Gly Val Val Gln Tyr Gly 275 280 285 Leu Val Asp Pro Lys Val Asp Thr Thr Tyr Pro Leu Glu Gln Ala Gly 290 295 300 Lys Ala Leu Ala His Val Glu Gln Gly His Ala Arg Gly Lys Ile Val 305 310 315 320 Leu Glu Leu Ile Thr Ser Gln Asp 325 <210> 83 <211> 1231 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (1201) <223> RXA01849 <400> 83 aaaaccttaa gttgggtggt taaacccact aaggtctcac tttatggatg tgccaggtca 60 caccaaaaaa tctcaagaaa actcacatta aaggacagta atg gcg tca caa cag 115 Met Ala Ser Gln Gln 1 5 atc cgc tat cca ttc tcc gcg gtt gtg gga caa gac gag ctt cgg ctt 163 Ile Arg Tyr Pro Phe Ser Ala Val Val Gly Gln Asp Glu Leu Arg Leu 10 15 20 gcg ttg atc ctc act gcg att tcc cca cgc att ggt ggc gtg gtg att 211 Ala Leu Ile Leu Thr Ala Ile Ser Pro Arg Ile Gly Gly Val Val Ile 25 30 35 cga ggt gag aag ggt aca gcg aaa act acc act gtg cgt gct ttt gct 259 Arg Gly Glu Lys Gly Thr Ala Lys Thr Thr Thr Val Val Arg Ala Phe Ala 40 45 50 ggt ctt tta ggt gat gcc cct ttg gtg aac ttg cct ctc gga tcc acg 307 Gly Leu Leu Gly Asp Ala Pro Leu Val Asn Leu Pro Leu Gly Ser Thr 55 60 65 gag gat cgt gtg gtg ggt tcc ctc aac atg gaa act gtg ttg acc acc 355 Glu Asp Arg Val Val Gly Ser Leu Asn Met Glu Thr Val Leu Thr Thr 70 75 80 85 ggc cgt gcg gaa tat cag cca ggt ttg ctc gcg cag gct gat ggc ggt 403 Gly Arg Ala Glu Tyr Gln Pro Gly Leu Leu Ala Gln Ala Asp Gly Gly 90 95 100 gtg ctg tat gtc gat gag gtc aac ctc ttg gcg gat cac ctg gtg gat 451 Val Leu Tyr Val Asp Glu Val Asn Leu Leu Ala Asp His Leu Val Asp 105 110 115 gct ctg ctc gat gca gct gca agc ggt cgc gtc agc att gag cgt gac 499 Ala Leu Leu Asp Ala Ala Ala Ser Gly Arg Val Ser Ile Glu Arg Asp 120 125 130 ggt att tcg cat tct tca cca gca aac ttt gtg ttg gtg ggc acc atg 547 Gly Ile Ser His Ser Ser Pro Ala Asn Phe Val Leu Val Gly Thr Met 135 140 145 aat ccg gag gaa ggc gag ctg cgc ccg cag ctg ctg gac cgt ttc ggt 595 Asn Pro Glu Glu Gly Glu Leu Arg Pro Gln Leu Leu Asp Arg Phe Gly 150 155 160 165 ttg gct gtg gac gtt gct gcg tct acg aac cct gag gtg cgc gtg gag 643 Leu Ala Val Asp Val Ala Ala Ser Thr Asn Pro Glu Val Arg Val Glu 170 175 180 atc att cgc cgc cgg ctt gat ttt gaa aac gct cct gag cag ttc atg 691 Ile Ile Arg Arg Arg Leu Asp Phe Glu Asn Ala Pro Glu Gln Phe Met 185 190 195 gct aag tgg gct gag caa gat gcg gac acc tcc aac cgt att ttg gcg 739 Ala Lys Trp Ala Glu Gln Asp Ala Asp Thr Ser Asn Arg Ile Leu Ala 200 205 210 gct aag gat ttg ctg cct ggt gtg gag ctg ccg gat ctg atc ttg tcg 787 Ala Lys Asp Leu Leu Pro Gly Val Glu Leu Pro Asp Leu Ile Leu Ser 215 220 225 cag att gcg tgg ttg tgt gca cgt att gaa gtc gac ggt atg cgc gct 835 Gln Ile Ala Trp Leu Cys Ala Arg Ile Glu Val Asp Gly Met Arg Ala 230 235 240 245 gac ctg gtg atc acg cgt acc gca ctt gct cac gcc gcg tgg gct gga 883 Asp Leu Val Ile Thr Arg Thr Ala Leu Ala His Ala Ala Trp Ala Gly 250 255 260 cgc act gtg gtt acg gaa gaa gac gtg gag atc gca gct cgc cta gcg 931 Arg Thr Val Val Thr Glu Glu Asp Val Glu Ile Ala Ala Arg Leu Ala 265 270 275 ttg ccg cac cgc cgt cgc cgt aat cct ttc gat gct cca gaa atg gag 979 Leu Pro His Arg Arg Arg Arg Asn Pro Phe Asp Ala Pro Glu Met Glu 280 285 290 gag cgc aag ctt cag gaa acc ctg cag gaa gct cgg gac ttc ttc aaa 1027 Glu Arg Lys Leu Gln Glu Thr Leu Gln Glu Ala Arg Asp Phe Phe Lys 295 300 305 gac aat gaa gat aaa gga cct gcc gcc aag atc acc gat gag gaa acc 1075 Asp Asn Glu Asp Lys Gly Pro Ala Ala Lys Ile Thr Asp Glu Glu Thr 310 315 320 325 ggt gca gag gcc ttt acc gat acc gac aat ccc acc gag gaa gac ggt 1123 Gly Ala Glu Ala Phe Thr Asp Thr Asp Asn Pro Thr Glu Glu Asp Gly 330 335 340 ctg caa gga act gcg cag gcg aag gcg cag act act gga aag gta ggt 1171 Leu Gln Gly Thr Ala Gln Ala Lys Ala Gln Thr Thr Gly Lys Val Gly 345 350 355 act gcc gga tcc ggc gac ccc ttt cgc tcc taggcatttg cgcctggcgt 1221 Thr Ala Gly Ser Gly Asp Pro Phe Arg Ser 360 365 ccatggggga 1231 <210> 84 <211> 367 <212> PRT <213> Corynebacterium glutamicum <400> 84 Met Ala Ser Gln Gln Ile Arg Tyr Pro Phe Ser Ala Val Val Gly Gln 1 5 10 15 Asp Glu Leu Arg Leu Ala Leu Ile Leu Thr Ala Ile Ser Pro Arg Ile 20 25 30 Gly Gly Val Val Ile Arg Gly Glu Lys Gly Thr Ala Lys Thr Thr Thr 35 40 45 Val Arg Ala Phe Ala Gly Leu Leu Gly Asp Ala Pro Leu Val Asn Leu 50 55 60 Pro Leu Gly Ser Thr Glu Asp Arg Val Val Gly Ser Leu Asn Met Glu 65 70 75 80 Thr Val Leu Thr Thr Gly Arg Ala Glu Tyr Gln Pro Gly Leu Leu Ala 85 90 95 Gln Ala Asp Gly Gly Val Leu Tyr Val Asp Glu Val Asn Leu Leu Ala 100 105 110 Asp His Leu Val Asp Ala Leu Leu Asp Ala Ala Ala Ser Gly Arg Val 115 120 125 Ser Ile Glu Arg Asp Gly Ile Ser His Ser Ser Pro Ala Asn Phe Val 130 135 140 Leu Val Gly Thr Met Asn Pro Glu Glu Gly Glu Leu Arg Pro Gln Leu 145 150 155 160 Leu Asp Arg Phe Gly Leu Ala Val Asp Val Ala Ala Ser Thr Asn Pro 165 170 175 Glu Val Arg Val Glu Ile Ile Arg Arg Arg Leu Asp Phe Glu Asn Ala 180 185 190 Pro Glu Gln Phe Met Ala Lys Trp Ala Glu Gln Asp Ala Asp Thr Ser 195 200 205 Asn Arg Ile Leu Ala Ala Lys Asp Leu Leu Pro Gly Val Glu Leu Pro 210 215 220 Asp Leu Ile Leu Ser Gln Ile Ala Trp Leu Cys Ala Arg Ile Glu Val 225 230 235 240 Asp Gly Met Arg Ala Asp Leu Val Ile Thr Arg Thr Ala Leu Ala His 245 250 255 Ala Ala Trp Ala Gly Arg Thr Val Val Thr Glu Glu Asp Val Glu Ile 260 265 270 Ala Ala Arg Leu Ala Leu Pro His Arg Arg Arg Arg Asn Pro Phe Asp 275 280 285 Ala Pro Glu Met Glu Glu Arg Lys Leu Gln Glu Thr Leu Gln Glu Ala 290 295 300 Arg Asp Phe Phe Lys Asp Asn Glu Asp Lys Gly Pro Ala Ala Lys Ile 305 310 315 320 Thr Asp Glu Glu Thr Gly Ala Glu Ala Phe Thr Asp Thr Asp Asn Pro 325 330 335 Thr Glu Glu Asp Gly Leu Gln Gly Thr Ala Gln Ala Lys Ala Gln Thr 340 345 350 Thr Gly Lys Val Gly Thr Ala Gly Ser Gly Asp Pro Phe Arg Ser 355 360 365 <210> 85 <211> 907 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (877) <223> RXA02016 <400> 85 acatggagct catccgcagc ggtccaccag cagaaatggt gggcatcggc acgcctctac 60 cgttccccac ctcacaaccg gacattcact aggacacact atg tca aac ttt gaa 115 Met Ser Asn Phe Glu 1 5 acg ttc ctc tgg gtt gcc tac ccc tgg ctg tgt atc gcc gcc tac atc 163 Thr Phe Leu Trp Val Ala Tyr Pro Trp Leu Cys Ile Ala Ala Tyr Ile 10 15 20 atc ggc att tct tgg cgc tgg cgc gcc gac caa ttc ggt tgg acc acc 211 Ile Gly Ile Ser Trp Arg Trp Arg Ala Asp Gln Phe Gly Trp Thr Thr 25 30 35 cac tcc tcc caa atc tac gaa tcc aaa ctc ctc cgc atc gcc tcc cca 259 His Ser Ser Gln Ile Tyr Glu Ser Lys Leu Leu Arg Ile Ala Ser Pro 40 45 50 ctc ttc cac tgg ggc atg gtg ttc gtg gtg atc ggc cac ctc atg gga 307 Leu Phe His Trp Gly Met Val Phe Val Val Ile Gly His Leu Met Gly 55 60 65 ctt gcc atc ccc aag agc tgg acc caa gct gta gga att tct gac gcc 355 Leu Ala Ile Pro Lys Ser Trp Thr Gln Ala Val Gly Ile Ser Asp Ala 70 75 80 85 gct tac cac ctc atc gcc acc atc cca ggc acc att gcc ggc atc gct 403 Ala Tyr His Leu Ile Ala Thr Ile Pro Gly Thr Ile Ala Gly Ile Ala 90 95 100 gca gtc ctt gga ctc atc ggc ttg att atc cgt cgc gtg atc aac aaa 451 Ala Val Leu Gly Leu Ile Gly Leu Ile Ile Arg Arg Val Ile Asn Lys 105 110 115 acc gtc ttc ctg tcc acc tca cgc tcc gac aaa gtg atg tat gtg cta 499 Thr Val Phe Leu Ser Thr Ser Arg Ser Asp Lys Val Met Tyr Val Leu 120 125 130 ctc ggc gct gca att ttg tcc ggt ttc atc gcc acc gtc tcc acc cag 547 Leu Gly Ala Ala Ile Leu Ser Gly Phe Ile Ala Thr Val Ser Thr Gln 135 140 145 gtc ttc ggc ggc gca cac ggc tac gac tac cgc gaa acc atc tcc cca 595 Val Phe Gly Gly Ala His Gly Tyr Asp Tyr Arg Glu Thr Ile Ser Pro 150 155 160 165 tgg gtg cgc caa ctg ctc atc ttc aac gct caa cca gag ctc atg gct 643 Trp Val Arg Gln Leu Leu Ile Phe Asn Ala Gln Pro Glu Leu Met Ala 170 175 180 gat gtc cct tgg gaa ttc aag gtc cac atc gtc gct gga ttc acc ctc 691 Asp Val Pro Trp Glu Phe Lys Val His Ile Val Ala Gly Phe Thr Leu 185 190 195 atc gca ctg tgg cca ttc acc cgc cta gtc cac gcg ttc tcc gca cca 739 Ile Ala Leu Trp Pro Phe Thr Arg Leu Val His Ala Phe Ser Ala Pro 200 205 210 gtt gga tac gtc acc cgc ccc tac gtg gtc tat cgc acc cgc gac acc 787 Val Gly Tyr Val Thr Arg Pro Tyr Val Val Tyr Arg Thr Arg Asp Thr 215 220 225 acc tct gaa ccg gca cgc caa aac gtc gcc tgg gaa ccg atc cgc tcg 835 Thr Ser Glu Pro Ala Arg Gln Asn Val Ala Trp Glu Pro Ile Arg Ser 230 235 240 245 gtc aaa aat cag ctc gac aat gac tcg aaa tgg cac ggc gcc 877 Val Lys Asn Gln Leu Asp Asn Asp Ser Lys Trp His Gly Ala 250 255 taaattcctc acaagccccc tagttaatct 907 <210> 86 <211> 259 <212> PRT <213> Corynebacterium glutamicum <400> 86 Met Ser Asn Phe Glu Thr Phe Leu Trp Val Ala Tyr Pro Trp Leu Cys 1 5 10 15 Ile Ala Ala Tyr Ile Ile Gly Ile Ser Trp Arg Trp Arg Ala Asp Gln 20 25 30 Phe Gly Trp Thr Thr His Ser Ser Gln Ile Tyr Glu Ser Lys Leu Leu 35 40 45 Arg Ile Ala Ser Pro Leu Phe His Trp Gly Met Val Phe Val Val Ile 50 55 60 Gly His Leu Met Gly Leu Ala Ile Pro Lys Ser Trp Thr Gln Ala Val 65 70 75 80 Gly Ile Ser Asp Ala Ala Tyr His Leu Ile Ala Thr Ile Pro Gly Thr 85 90 95 Ile Ala Gly Ile Ala Ala Val Leu Gly Leu Ile Gly Leu Ile Ile Arg 100 105 110 Arg Val Ile Asn Lys Thr Val Phe Leu Ser Thr Ser Arg Ser Asp Lys 115 120 125 Val Met Tyr Val Leu Leu Gly Ala Ala Ile Leu Ser Gly Phe Ile Ala 130 135 140 Thr Val Ser Thr Gln Val Phe Gly Gly Ala His Gly Tyr Asp Tyr Arg 145 150 155 160 Glu Thr Ile Ser Pro Trp Val Arg Gln Leu Leu Ile Phe Asn Ala Gln 165 170 175 Pro Glu Leu Met Ala Asp Val Pro Trp Glu Phe Lys Val His Ile Val 180 185 190 Ala Gly Phe Thr Leu Ile Ala Leu Trp Pro Phe Thr Arg Leu Val His 195 200 205 Ala Phe Ser Ala Pro Val Gly Tyr Val Thr Arg Pro Tyr Val Val Tyr 210 215 220 Arg Thr Arg Asp Thr Thr Ser Glu Pro Ala Arg Gln Asn Val Ala Trp 225 230 235 240 Glu Pro Ile Arg Ser Val Lys Asn Gln Leu Asp Asn Asp Ser Lys Trp 245 250 255 His Gly Ala <210> 87 <211> 1078 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (1048) <223> RXA02021 <400> 87 ttgggtcgcc gaggagatct aatcctggtt tgagttcaga gttcacaggt ttaagcctac 60 aaaccttagt taaaacatga tggaagcggt cgattaaaaa atg agt gaa aac att 115 Met Ser Glu Asn Ile 1 5 cgc gga gcc caa gca gtt gga atc gca aat atc gcc atg gac ggg acc 163 Arg Gly Ala Gln Ala Val Gly Ile Ala Asn Ile Ala Met Asp Gly Thr 10 15 20 atc ctg gac acg tgg tac cca gaa ccc caa att ttc aac ccg gat cag 211 Ile Leu Asp Thr Trp Tyr Pro Glu Pro Gln Ile Phe Asn Pro Asp Gln 25 30 35 tgg gct gaa cgc tac cca ttg gaa gtg ggc acc aca cgc ctc gga gca 259 Trp Ala Glu Arg Tyr Pro Leu Glu Val Gly Thr Thr Arg Leu Gly Ala 40 45 50 aac gaa ctc acc cca cgg atg ctg cag ttg gta aaa ctg gac caa gat 307 Asn Glu Leu Thr Pro Arg Met Leu Gln Leu Val Lys Leu Asp Gln Asp 55 60 65 cgc ctc gtc gaa cag gta gca gtc cgc acc gtt atc ccc gat ctg tct 355 Arg Leu Val Glu Gln Val Ala Val Arg Thr Val Ile Pro Asp Leu Ser 70 75 80 85 caa cct cca gta gac gcg cac gat gtt tac ctg cgc ctc cac ctg ctt 403 Gln Pro Pro Val Asp Ala His Asp Val Tyr Leu Arg Leu His Leu Leu 90 95 100 tcc cac cgc ctg gtc cgc ccc cac gaa atg cac atg caa aac acc ttg 451 Ser His Arg Leu Val Arg Pro His Glu Met His Met Gln Asn Thr Leu 105 110 115 gag ctg ctg tcc gac gtg gtg tgg aca aac aag ggc cct tgc ctt cct 499 Glu Leu Leu Ser Asp Val Val Trp Thr Asn Lys Gly Pro Cys Leu Pro 120 125 130 gaa aac ttt gag tgg gtg cgt ggt gct ctg cgg tcc cgc gga ctc atc 547 Glu Asn Phe Glu Trp Val Arg Gly Ala Leu Arg Ser Arg Gly Leu Ile 135 140 145 cac gtc tac tgt gtg gac cgt ctt ccc cgc atg gtc gac tat gtg gtt 595 His Val Tyr Cys Val Asp Arg Leu Pro Arg Met Val Asp Tyr Val Val 150 155 160 165 ccc cct gga gtc cgc atc tcc gaa gca gaa cgc gtg cgc cta ggt gca 643 Pro Pro Gly Val Arg Ile Ser Glu Ala Glu Arg Val Arg Leu Gly Ala 170 175 180 tac ctt gct ccg ggt acc tct gtg ctg cgt gaa ggt ttc gtg tct ttc 691 Tyr Leu Ala Pro Gly Thr Ser Val Leu Arg Glu Gly Phe Val Ser Phe 185 190 195 aac tcc ggc acc ttg ggt gcc gca aag gtg gaa ggc cgc ctg agt tcc 739 Asn Ser Gly Thr Leu Gly Ala Ala Lys Val Glu Gly Arg Leu Ser Ser 200 205 210 ggt gtg gtc atc ggt gaa ggt tcc gag att gga ctg tct tct act att 787 Gly Val Val Ile Gly Glu Gly Ser Glu Ile Gly Leu Ser Ser Thr Ile 215 220 225 cag tcc ccg aga gat gaa cag cgc cgc cgt ttg ccg ttg agc atc ggc 835 Gln Ser Pro Arg Asp Glu Gln Arg Arg Arg Leu Pro Leu Ser Ile Gly 230 235 240 245 caa aac tgc aac ttt ggt gtc agc tcc gga atc atc gga gtc agt ctg 883 Gln Asn Cys Asn Phe Gly Val Ser Ser Gly Ile Ile Gly Val Ser Leu 250 255 260 gga gac aat tgc gac atc gga aat aac att gtc ttg gat gga gat acc 931 Gly Asp Asn Cys Asp Ile Gly Asn Asn Ile Val Leu Asp Gly Asp Thr 265 270 275 ccc att tgg ttc gca gcc gat gag gag tta cgc act atc gac tcc atc 979 Pro Ile Trp Phe Ala Ala Asp Glu Glu Leu Arg Thr Ile Asp Ser Ile 280 285 290 gaa ggc caa gca aat tgg tca atc aag cgt gaa tcc ggc ttc cat gag 1027 Glu Gly Gln Ala Asn Trp Ser Ile Lys Arg Glu Ser Gly Phe His Glu 295 300 305 cca gtt gcc cgc ctc aaa gct tgacccattt tcataaccag tgccatgtgg 1078 Pro Val Ala Arg Leu Lys Ala 310 315 <210> 88 <211> 316 <212> PRT <213> Corynebacterium glutamicum <400> 88 Met Ser Glu Asn Ile Arg Gly Ala Gln Ala Val Gly Ile Ala Asn Ile 1 5 10 15 Ala Met Asp Gly Thr Ile Leu Asp Thr Trp Tyr Pro Glu Pro Gln Ile 20 25 30 Phe Asn Pro Asp Gln Trp Ala Glu Arg Tyr Pro Leu Glu Val Gly Thr 35 40 45 Thr Arg Leu Gly Ala Asn Glu Leu Thr Pro Arg Met Leu Gln Leu Val 50 55 60 Lys Leu Asp Gln Asp Arg Leu Val Glu Gln Val Ala Val Arg Thr Val 65 70 75 80 Ile Pro Asp Leu Ser Gln Pro Pro Val Asp Ala His Asp Val Tyr Leu 85 90 95 Arg Leu His Leu Leu Ser His Arg Leu Val Arg Pro His Glu Met His 100 105 110 Met Gln Asn Thr Leu Glu Leu Leu Ser Asp Val Val Trp Thr Asn Lys 115 120 125 Gly Pro Cys Leu Pro Glu Asn Phe Glu Trp Val Arg Gly Ala Leu Arg 130 135 140 Ser Arg Gly Leu Ile His Val Tyr Cys Val Asp Arg Leu Pro Arg Met 145 150 155 160 Val Asp Tyr Val Val Pro Pro Gly Val Arg Ile Ser Glu Ala Glu Arg 165 170 175 Val Arg Leu Gly Ala Tyr Leu Ala Pro Gly Thr Ser Val Leu Arg Glu 180 185 190 Gly Phe Val Ser Phe Asn Ser Gly Thr Leu Gly Ala Ala Lys Val Glu 195 200 205 Gly Arg Leu Ser Ser Gly Val Val Ile Gly Glu Gly Ser Glu Ile Gly 210 215 220 Leu Ser Ser Thr Ile Gln Ser Pro Arg Asp Glu Gln Arg Arg Arg Leu 225 230 235 240 Pro Leu Ser Ile Gly Gln Asn Cys Asn Phe Gly Val Ser Ser Gly Ile 245 250 255 Ile Gly Val Ser Leu Gly Asp Asn Cys Asp Ile Gly Asn Asn Ile Val 260 265 270 Leu Asp Gly Asp Thr Pro Ile Trp Phe Ala Ala Asp Glu Glu Leu Arg 275 280 285 Thr Ile Asp Ser Ile Glu Gly Gln Ala Asn Trp Ser Ile Lys Arg Glu 290 295 300 Ser Gly Phe His Glu Pro Val Ala Arg Leu Lys Ala 305 310 315 <210> 89 <211> 709 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (679) <223> RXA02053 <400> 89 aaccagccag aaactatctc caaaagctaa taaaaccctt gcactgacaa ataaggcgac 60 ctaccatgac tctgtttcca acacataaaa aggataaaaa atg tca ctt tca gtc 115 Met ser leu ser val 1 5 gtc gag gcg att acc aac cgc cgc gcc acc cgc aaa tac acc gat gaa 163 Val Glu Ala Ile Thr Asn Arg Arg Ala Thr Arg Lys Tyr Thr Asp Glu 10 15 20 gct cct acc cct gag ctg atc gac aaa atc gtt gac ctt gcc ctg gag 211 Ala Pro Thr Pro Glu Leu Ile Asp Lys Ile Val Asp Leu Ala Leu Glu 25 30 35 gca ccc agt gcg ttc aat gcg cag caa cgt gaa att gtt gtg att act 259 Ala Pro Ser Ala Phe Asn Ala Gln Gln Arg Glu Ile Val Val Ile Thr 40 45 50 gat ccc gca cag aag cag aag ctt tac gag gcc tcc cat cag aaa caa 307 Asp Pro Ala Gln Lys Gln Lys Leu Tyr Glu Ala Ser His Gln Lys Gln 55 60 65 ttc ctc acc gca cct gta act ttc att gcg gtt gcc cgc gtg gaa aac 355 Phe Leu Thr Ala Pro Val Thr Phe Ile Ala Val Ala Arg Val Glu Asn 70 75 80 85 gag cct gag gat ttg gaa gag att ctt ggt acg gaa agg gct gaa cgt 403 Glu Pro Glu Asp Leu Glu Glu Ile Leu Gly Thr Glu Arg Ala Glu Arg 90 95 100 gtc gcg gga ttc atc aac ggt cgc agc att cag cag gca cgc gaa gca 451 Val Ala Gly Phe Ile Asn Gly Arg Ser Ile Gln Gln Ala Arg Glu Ala 105 110 115 acg ttg agg gat gcc agc ctc gcg gcg gct ttt cta att ctg gct gcc 499 Thr Leu Arg Asp Ala Ser Leu Ala Ala Ala Phe Leu Ile Leu Ala Ala 120 125 130 cag gcg gag ggt ttg agt acc agc ccg act act ggt tgg gat gag gaa 547 Gln Ala Glu Gly Leu Ser Thr Ser Pro Thr Thr Gly Trp Asp Glu Glu 135 140 145 aaa gtg aag gaa gca atc ggt ctc ggc ggg cgt gag gat cgt gca atc 595 Lys Val Lys Glu Ala Ile Gly Leu Gly Gly Arg Glu Asp Arg Ala Ile 150 155 160 165 gcc ctt gtt att gct acc gga ttc cct aat gaa cag ccg gag cac cct 643 Ala Leu Val Ile Ala Thr Gly Phe Pro Asn Glu Gln Pro Glu His Pro 170 175 180 ggt cgt ttg cag aat agg cgc atc gac aac agc tac taactctgcc 689 Gly Arg Leu Gln Asn Arg Arg Ile Asp Asn Ser Tyr 185 190 agctcgcccg gacgaactaa 709 <210> 90 <211> 193 <212> PRT <213> Corynebacterium glutamicum <400> 90 Met Ser Leu Ser Val Val Glu Ala Ile Thr Asn Arg Arg Ala Thr Arg 1 5 10 15 Lys Tyr Thr Asp Glu Ala Pro Thr Pro Glu Leu Ile Asp Lys Ile Val 20 25 30 Asp Leu Ala Leu Glu Ala Pro Ser Ala Phe Asn Ala Gln Gln Arg Glu 35 40 45 Ile Val Val Ile Thr Asp Pro Ala Gln Lys Gln Lys Leu Tyr Glu Ala 50 55 60 Ser His Gln Lys Gln Phe Leu Thr Ala Pro Val Thr Phe Ile Ala Val 65 70 75 80 Ala Arg Val Glu Asn Glu Pro Glu Asp Leu Glu Glu Ile Leu Gly Thr 85 90 95 Glu Arg Ala Glu Arg Val Ala Gly Phe Ile Asn Gly Arg Ser Ile Gln 100 105 110 Gln Ala Arg Glu Ala Thr Leu Arg Asp Ala Ser Leu Ala Ala Ala Phe 115 120 125 Leu Ile Leu Ala Ala Gln Ala Glu Gly Leu Ser Thr Ser Pro Thr Thr 130 135 140 Gly Trp Asp Glu Glu Lys Val Lys Glu Ala Ile Gly Leu Gly Gly Arg 145 150 155 160 Glu Asp Arg Ala Ile Ala Leu Val Ile Ala Thr Gly Phe Pro Asn Glu 165 170 175 Gln Pro Glu His Pro Gly Arg Leu Gln Asn Arg Arg Ile Asp Asn Ser 180 185 190 Tyr <210> 91 <211> 769 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (739) <223> RXA02064 <400> 91 ctagattaca tcctgtgcat cttctggcaa ttgtgaattc atttgcactt aatgcgtgta 60 ggtacttcac atctcgcctg ctcgctctag actataacgc gtg agt aac gcc ttc 115 Val Ser Asn Ala Phe 1 5 gag tat ctt cgc act tat gtc gag tcc act act gaa acc gac gct gct 163 Glu Tyr Leu Arg Thr Tyr Val Glu Ser Thr Thr Glu Thr Asp Ala Ala 10 15 20 gta gcg cgc gct cgt gaa gac gcc gcc gag ttc ggt ctc ccc gcc ccg 211 Val Ala Arg Ala Arg Glu Asp Ala Ala Glu Phe Gly Leu Pro Ala Pro 25 30 35 gat gaa atg act ggc cag ctg cta acc act ttg gct gcc acc acc aat 259 Asp Glu Met Thr Gly Gln Leu Leu Thr Thr Leu Ala Ala Thr Thr Asn 40 45 50 ggc aac ggc tcc act ggt gcc atc gcg att acc ccg gct gcc ggg ttg 307 Gly Asn Gly Ser Thr Gly Ala Ile Ala Ile Thr Pro Ala Ala Gly Leu 55 60 65 gtg ggt ctg tat atc ctg aac gga ctg gcc gat aac acc aca ctg acc 355 Val Gly Leu Tyr Ile Leu Asn Gly Leu Ala Asp Asn Thr Thr Leu Thr 70 75 80 85 tgc att gat cct gaa tca gag cat cag cgc cag gcc aaa gca ctc ttc 403 Cys Ile Asp Pro Glu Ser Glu His Gln Arg Gln Ala Lys Ala Leu Phe 90 95 100 cgc gag gcc ggc tat tcc ccc agc cgc gta cgc ttc ttg ctc tcg cgc 451 Arg Glu Ala Gly Tyr Ser Pro Ser Arg Val Arg Phe Leu Leu Ser Arg 105 110 115 ccg ctc gac gtg atg agt cgc ctt gcc aac gac agc tat cag ctt gtc 499 Pro Leu Asp Val Met Ser Arg Leu Ala Asn Asp Ser Tyr Gln Leu Val 120 125 130 ttc ggc caa gtc tcc ccc atg gat tta aag gcg ctt gtc gac gcc gcc 547 Phe Gly Gln Val Ser Pro Met Asp Leu Lys Ala Leu Val Asp Ala Ala 135 140 145 tgg ccg ctt ctt cga cga ggc ggt gcg ctg gtg ctc gcc gat gcg ctc 595 Trp Pro Leu Leu Arg Arg Gly Gly Ala Leu Val Leu Ala Asp Ala Leu 150 155 160 165 ctt gac ggc acc att gcg gat caa acc cgc aag gat cgt gac acc caa 643 Leu Asp Gly Thr Ile Ala Asp Gln Thr Arg Lys Asp Arg Asp Thr Gln 170 175 180 gca gca cgt gac gcc gat gaa tat att cgt tcc att gaa ggc gca cac 691 Ala Ala Arg Asp Ala Asp Glu Tyr Ile Arg Ser Ile Glu Gly Ala His 185 190 195 gtt gct cgc ctg ccc ctt ggt gca ggc tta acc gtg gtg act aaa gcc 739 Val Ala Arg Leu Pro Leu Gly Ala Gly Leu Thr Val Val Thr Lys Ala 200 205 210 tagaggggga cgtcgagaag cgaaaaaagg 769 <210> 92 <211> 213 <212> PRT <213> Corynebacterium glutamicum <400> 92 Val Ser Asn Ala Phe Glu Tyr Leu Arg Thr Tyr Val Glu Ser Thr Thr 1 5 10 15 Glu Thr Asp Ala Ala Val Ala Arg Ala Arg Glu Asp Ala Ala Glu Phe 20 25 30 Gly Leu Pro Ala Pro Asp Glu Met Thr Gly Gln Leu Leu Thr Thr Leu 35 40 45 Ala Ala Thr Thr Asn Gly Asn Gly Ser Thr Gly Ala Ile Ala Ile Thr 50 55 60 Pro Ala Ala Gly Leu Val Gly Leu Tyr Ile Leu Asn Gly Leu Ala Asp 65 70 75 80 Asn Thr Thr Leu Thr Cys Ile Asp Pro Glu Ser Glu His Gln Arg Gln 85 90 95 Ala Lys Ala Leu Phe Arg Glu Ala Gly Tyr Ser Pro Ser Arg Val Arg 100 105 110 Phe Leu Leu Ser Arg Pro Leu Asp Val Met Ser Arg Leu Ala Asn Asp 115 120 125 Ser Tyr Gln Leu Val Phe Gly Gln Val Ser Pro Met Asp Leu Lys Ala 130 135 140 Leu Val Asp Ala Ala Trp Pro Leu Leu Arg Arg Gly Gly Ala Leu Val 145 150 155 160 Leu Ala Asp Ala Leu Leu Asp Gly Thr Ile Ala Asp Gln Thr Arg Lys 165 170 175 Asp Arg Asp Thr Gln Ala Ala Arg Asp Ala Asp Glu Tyr Ile Arg Ser 180 185 190 Ile Glu Gly Ala His Val Ala Arg Leu Pro Leu Gly Ala Gly Leu Thr 195 200 205 Val Val Thr Lys Ala 210 <210> 93 <211> 1540 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1510) <223> RXA02083 <400> 93 tttttgtaca tggtctgtgg cacgcattgt ttcgtttgcc ctgccatgtt ggtgttttaa 60 gtgaaggccg ccttttgcgg aatgggtata ggaggcattc atg gag atg gtt atg 115 Met Glu Met Val Met 1 5 aag aat aag cgc gtt gcg att att ggt gca ggt ccg agt ggt atc gct 163 Lys Asn Lys Arg Val Ala Ile Ile Gly Ala Gly Pro Ser Gly Ile Ala 10 15 20 cag ttg agg gcg ttt gag tct gct gaa aag cag ggg cat gag atc cct 211 Gln Leu Arg Ala Phe Glu Ser Ala Glu Lys Gln Gly His Glu Ile Pro 25 30 35 gag ctg gtg tgt ttt gaa aag cag gat acc tgg ggt ggg cag tgg aat 259 Glu Leu Val Cys Phe Glu Lys Gln Asp Thr Trp Gly Gly Gln Trp Asn 40 45 50 tac tct tgg cgc acg gga aca gac tct tat ggt gag cct gtg cac tca 307 Tyr Ser Trp Arg Thr Gly Thr Asp Ser Tyr Gly Glu Pro Val His Ser 55 60 65 agt atg tac cga aac ctg tgg tca aac ggt ccg aag gaa gtt ctc gaa 355 Ser Met Tyr Arg Asn Leu Trp Ser Asn Gly Pro Lys Glu Val Leu Glu 70 75 80 85 ttt gct gag tac agc ttc gat gag cac ttc gga aag cca att tct tct 403 Phe Ala Glu Tyr Ser Phe Asp Glu His Phe Gly Lys Pro Ile Ser Ser 90 95 100 tac cct cca cgt gaa gtg ttg tgg gat tac att gca ggt cgt gca aag 451 Tyr Pro Pro Arg Glu Val Leu Trp Asp Tyr Ile Ala Gly Arg Ala Lys 105 110 115 aag tcg aac gtt gag aag tac atc aag ttc gcg cat gtt gtt cgc tgg 499 Lys Ser Asn Val Glu Lys Tyr Ile Lys Phe Ala His Val Val Arg Trp 120 125 130 gtg agt ttt gat gag gcc acc aag ctg ttc acc gtg acg gtg gag aac 547 Val Ser Phe Asp Glu Ala Thr Lys Leu Phe Thr Val Thr Val Glu Asn 135 140 145 ctc cgc acc ggt gag acc agc agt gat act tat gac aac gtg att gtt 595 Leu Arg Thr Gly Glu Thr Ser Ser Asp Thr Tyr Asp Asn Val Ile Val 150 155 160 165 ggc gct gga cac ttc agc ttc ccg aac gtc cct cac ttt gat ggt gtg 643 Gly Ala Gly His Phe Ser Phe Pro Asn Val Pro His Phe Asp Gly Val 170 175 180 gag act ttc cca ggt cag atc atg cat gct cac gag ttc cgt ggt gca 691 Glu Thr Phe Pro Gly Gln Ile Met His Ala His Glu Phe Arg Gly Ala 185 190 195 gag gct gtt gct gac aag gat att ttg ctg att ggt gca agt tat tct 739 Glu Ala Val Ala Asp Lys Asp Ile Leu Leu Ile Gly Ala Ser Tyr Ser 200 205 210 gcg gaa gat atc ggt acc cag gcg tac aag atg ggt gct cgt tcg gtg 787 Ala Glu Asp Ile Gly Thr Gln Ala Tyr Lys Met Gly Ala Arg Ser Val 215 220 225 act ttc tct tac cgc tca aac cca atg ggg tat gag tgg cct gaa gag 835 Thr Phe Ser Tyr Arg Ser Asn Pro Met Gly Tyr Glu Trp Pro Glu Glu 230 235 240 245 atg act gag ctt cct ttg gtt gag cgt ttc gac ggc tcc gag gtt cac 883 Met Thr Glu Leu Pro Leu Val Glu Arg Phe Asp Gly Ser Glu Val His 250 255 260 ttt gtc aat ggt gaa aag cgc aag gtc gac atc gtg gtg ttc tgt act 931 Phe Val Asn Gly Glu Lys Arg Lys Val Asp Ile Val Val Phe Cys Thr 265 270 275 ggt tac tta cac cat tac cca ttt atg ccg tct gag ctg act tta agc 979 Gly Tyr Leu His His Tyr Pro Phe Met Pro Ser Glu Leu Thr Leu Ser 280 285 290 tca cca aac aac ctg tac ccg gat acg ctt tat cgt ggc gtg gtg tcc 1027 Ser Pro Asn Asn Leu Tyr Pro Asp Thr Leu Tyr Arg Gly Val Val Ser 295 300 305 gag gct aat aac cag ctg ttc tgg ttg ggt gct cag gat cag tgg ctg 1075 Glu Ala Asn Asn Gln Leu Phe Trp Leu Gly Ala Gln Asp Gln Trp Leu 310 315 320 325 acg ttc aac atg ttt gat gct cag gct tgg tat gtt cgc gat gtc att 1123 Thr Phe Asn Met Phe Asp Ala Gln Ala Trp Tyr Val Arg Asp Val Ile 330 335 340 ttg ggc cgc gtg gct ctt cct tcc aag gaa gcg cag cgc aat cat atg 1171 Leu Gly Arg Val Ala Leu Pro Ser Lys Glu Ala Gln Arg Asn His Met 345 350 355 gat aag tgg ctg tca cgt ttc gaa ggc ttg aag tct gag aat gat cag 1219 Asp Lys Trp Leu Ser Arg Phe Glu Gly Leu Lys Ser Glu Asn Asp Gln 360 365 370 att gat ttc cag tgc gat tac gtc gag gat ctt atc gat cag acc gat 1267 Ile Asp Phe Gln Cys Asp Tyr Val Glu Asp Leu Ile Asp Gln Thr Asp 375 380 385 tat cct tcg ttt gat ctg aag gaa gtc gcg aat atc ttg aag ggc tgg 1315 Tyr Pro Ser Phe Asp Leu Lys Glu Val Ala Asn Ile Leu Lys Gly Trp 390 395 400 405 gtg aag tcg aag gag gag gat atc ctc aac tac cgt gat tac acc tat 1363 Val Lys Ser Lys Glu Glu Asp Ile Leu Asn Tyr Arg Asp Tyr Thr Tyr 410 415 420 act tcc gtg atg acg ggc acc acc tct gtt gaa cac cac act ccg tgg 1411 Thr Ser Val Met Thr Gly Thr Thr Ser Ser Glu His His Thr Pro Trp 425 430 435 atg att gag ttg gat gat tct ttg gag cgt tac ctc agt gag cca cag 1459 Met Ile Glu Leu Asp Asp Ser Leu Glu Arg Tyr Leu Ser Glu Pro Gln 440 445 450 gaa gat gaa gct cgt cag gtt tac cgt ggc aag aaa gtc cgc gat aaa 1507 Glu Asp Glu Ala Arg Gln Val Tyr Arg Gly Lys Lys Val Arg Asp Lys 455 460 465 gcc taaggaaaag gcctaagcag cccttgcata 1540 Ala 470 <210> 94 <211> 470 <212> PRT <213> Corynebacterium glutamicum <400> 94 Met Glu Met Val Met Lys Asn Lys Arg Val Ala Ile Ile Gly Ala Gly 1 5 10 15 Pro Ser Gly Ile Ala Gln Leu Arg Ala Phe Glu Ser Ala Glu Lys Gln 20 25 30 Gly His Glu Ile Pro Glu Leu Val Cys Phe Glu Lys Gln Asp Thr Trp 35 40 45 Gly Gly Gln Trp Asn Tyr Ser Trp Arg Thr Gly Thr Asp Ser Tyr Gly 50 55 60 Glu Pro Val His Ser Ser Met Tyr Arg Asn Leu Trp Ser Asn Gly Pro 65 70 75 80 Lys Glu Val Leu Glu Phe Ala Glu Tyr Ser Phe Asp Glu His Phe Gly 85 90 95 Lys Pro Ile Ser Ser Tyr Pro Pro Arg Glu Val Leu Trp Asp Tyr Ile 100 105 110 Ala Gly Arg Ala Lys Lys Ser Asn Val Glu Lys Tyr Ile Lys Phe Ala 115 120 125 His Val Val Arg Trp Val Ser Phe Asp Glu Ala Thr Lys Leu Phe Thr 130 135 140 Val Thr Val Glu Asn Leu Arg Thr Gly Glu Thr Ser Ser Asp Thr Tyr 145 150 155 160 Asp Asn Val Ile Val Gly Ala Gly His Phe Ser Phe Pro Asn Val Pro 165 170 175 His Phe Asp Gly Val Glu Thr Phe Pro Gly Gln Ile Met His Ala His 180 185 190 Glu Phe Arg Gly Ala Glu Ala Val Ala Asp Lys Asp Ile Leu Leu Ile 195 200 205 Gly Ala Ser Tyr Ser Ala Glu Asp Ile Gly Thr Gln Ala Tyr Lys Met 210 215 220 Gly Ala Arg Ser Val Thr Phe Ser Tyr Arg Ser Asn Pro Met Gly Tyr 225 230 235 240 Glu Trp Pro Glu Glu Met Thr Glu Leu Pro Leu Val Glu Arg Phe Asp 245 250 255 Gly Ser Glu Val His Phe Val Asn Gly Glu Lys Arg Lys Val Asp Ile 260 265 270 Val Val Phe Cys Thr Gly Tyr Leu His His Tyr Pro Phe Met Pro Ser 275 280 285 Glu Leu Thr Leu Ser Ser Pro Asn Asn Leu Tyr Pro Asp Thr Leu Tyr 290 295 300 Arg Gly Val Val Ser Glu Ala Asn Asn Gln Leu Phe Trp Leu Gly Ala 305 310 315 320 Gln Asp Gln Trp Leu Thr Phe Asn Met Phe Asp Ala Gln Ala Trp Tyr 325 330 335 Val Arg Asp Val Ile Leu Gly Arg Val Ala Leu Pro Ser Lys Glu Ala 340 345 350 Gln Arg Asn His Met Asp Lys Trp Leu Ser Arg Phe Glu Gly Leu Lys 355 360 365 Ser Glu Asn Asp Gln Ile Asp Phe Gln Cys Asp Tyr Val Glu Asp Leu 370 375 380 Ile Asp Gln Thr Asp Tyr Pro Ser Phe Asp Leu Lys Glu Val Ala Asn 385 390 395 400 Ile Leu Lys Gly Trp Val Lys Ser Lys Glu Glu Asp Ile Leu Asn Tyr 405 410 415 Arg Asp Tyr Thr Tyr Thr Ser Val Met Thr Gly Thr Thr Ser Val Glu 420 425 430 His His Thr Pro Trp Met Ile Glu Leu Asp Asp Ser Leu Glu Arg Tyr 435 440 445 Leu Ser Glu Pro Gln Glu Asp Glu Ala Arg Gln Val Tyr Arg Gly Lys 450 455 460 Lys Val Arg Asp Lys Ala 465 470 <210> 95 <211> 1987 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1957) <223> RXA02092 <400> 95 gttgtttccc tgtcgacgta gttgaccatg acagtgattt tatactcatg cggagcaaag 60 gaagagcaaa tgcctgattg tgtgggatat ggcttgatta ttg gtg gaa gtt ttc 115 Leu Val Glu Val Phe 1 5 ggg aaa ttc ctg ccc att tgt gtg cgg ttg gtt ttt gaa aat cct gta 163 Gly Lys Phe Leu Pro Ile Cys Val Arg Leu Val Phe Glu Asn Pro Val 10 15 20 cat gtc cgg ata gct gtg gat ttg ccg agc cga cag att ggt gac gat 211 His Val Arg Ile Ala Val Asp Leu Pro Ser Arg Gln Ile Gly Asp Asp 25 30 35 cct tgc gcg cat aac tgc ata ttc atc aaa gcg ttg ccg gtc acc tgc 259 Pro Cys Ala His Asn Cys Ile Phe Ile Lys Ala Leu Pro Val Thr Cys 40 45 50 ttc gtg cct agc ctg gga ggc atg aat agt tcg acg tgg aca cct cgg 307 Phe Val Pro Ser Leu Gly Gly Met Asn Ser Ser Thr Trp Thr Pro Arg 55 60 65 tac tca ccg cat atg gct aac agt gca gcg ggt tcg gtg ttg gcg gat 355 Tyr Ser Pro His Met Ala Asn Ser Ala Ala Gly Ser Val Leu Ala Asp 70 75 80 85 gag atg gtg gtt ccc acc tca aca ggg ttt gtt aaa ggc gtc aaa gga 403 Glu Met Val Val Pro Thr Ser Thr Gly Phe Val Lys Gly Val Lys Gly 90 95 100 cca ggg ctt aaa acc tgg agg gga att ccc tac ggc cga aac act ggc 451 Pro Gly Leu Lys Thr Trp Arg Gly Ile Pro Tyr Gly Arg Asn Thr Gly 105 110 115 gga aaa tat cgc ttc cgg gca ccc cgg ccc gcc aag aaa tgg gac ggc 499 Gly Lys Tyr Arg Phe Arg Ala Pro Arg Pro Ala Lys Lys Trp Asp Gly 120 125 130 gtg cgc gat tgc tca atg ttc ggt gaa gta gct tct cag cca acg tac 547 Val Arg Asp Cys Ser Met Phe Gly Glu Val Ala Ser Gln Pro Thr Tyr 135 140 145 tcc tgg aca gat aag att cgc ggt tca gaa gac tgc ctt aac ctc gat 595 Ser Trp Thr Asp Lys Ile Arg Gly Ser Glu Asp Cys Leu Asn Leu Asp 150 155 160 165 gtc gtg cgg cct gat tcc gaa gaa aag ctt cct gtt gtg gtg tat ctc 643 Val Val Arg Pro Asp Ser Glu Glu Lys Leu Pro Val Val Val Tyr Leu 170 175 180 cac ggc ggt tcc ttc atc atg ggc tca tca agc gaa aaa gcg ctg cgc 691 His Gly Gly Ser Phe Ile Met Gly Ser Ser Ser Glu Lys Ala Leu Arg 185 190 195 gga tat aac ctc gtc aca aac atg aat gtg gtc tac gtg tcc gtt aat 739 Gly Tyr Asn Leu Val Thr Asn Met Asn Val Val Tyr Val Ser Val Asn 200 205 210 ttc cgc ctc ggc gct ttg ggc tat cta gat ctg cgt tcc gtg ggg gag 787 Phe Arg Leu Gly Ala Leu Gly Tyr Leu Asp Leu Arg Ser Val Gly Glu 215 220 225 gat tgc gta gcc aac ccc gcg ctc cac gat cag ctc ctg gcc ctg cag 835 Asp Cys Val Ala Asn Pro Ala Leu His Asp Gln Leu Leu Ala Leu Gln 230 235 240 245 tgg gtc agc cgt aat atc gaa gca ttc ggt ggg gat cct gac aac gtc 883 Trp Val Ser Arg Asn Ile Glu Ala Phe Gly Gly Asp Pro Asp Asn Val 250 255 260 acc ctc atg ggc gaa tcc gcg ggc gct gca gca gtg gtt gca ctc atg 931 Thr Leu Met Gly Glu Ser Ala Gly Ala Ala Ala Val Val Ala Leu Met 265 270 275 tgt gtg ccc gct gca gga gga cta ttc cac cgc gcc atc gcc caa tcc 979 Cys Val Pro Ala Ala Gly Gly Leu Phe His Arg Ala Ile Ala Gln Ser 280 285 290 gcg ccg gtc atc tct gtg cac tca tct acc caa gca aaa ttc tgg gca 1027 Ala Pro Val Ile Ser Val His Ser Ser Thr Gln Ala Lys Phe Trp Ala 295 300 305 cgt gaa ctg atc tac cgc atg gca ttg ccc agg gaa acc acc ttg gat 1075 Arg Glu Leu Ile Tyr Arg Met Ala Leu Pro Arg Glu Thr Thr Leu Asp 310 315 320 325 gaa ctg cgc caa gaa tcc gcc gat gat cta gtg cgc gcc ggg cag tcg 1123 Glu Leu Arg Gln Glu Ser Ala Asp Asp Leu Val Arg Ala Gly Gln Ser 330 335 340 atg atg tgg cgc tcc ggc gaa ctg ctc caa ctc aac tct tgt tac ggg 1171 Met Met Trp Arg Ser Gly Glu Leu Leu Gln Leu Asn Ser Cys Tyr Gly 345 350 355 ccc acg gtg gat ggt tcc ttg cta cct gag cac ccg ctc acc atg ttt 1219 Pro Thr Val Asp Gly Ser Leu Leu Pro Glu His Pro Leu Thr Met Phe 360 365 370 gaa cag gga aga caa cac cgc att ccc ttc atg atc ggt act aat aac 1267 Glu Gln Gly Arg Gln His Arg Ile Pro Phe Met Ile Gly Thr Asn Asn 375 380 385 gga gaa act tct ttc tcc aaa gcc ttt tat tta aga agc tct gcc cga 1315 Gly Glu Thr Ser Phe Ser Lys Ala Phe Tyr Leu Arg Ser Ser Ala Arg 390 395 400 405 cgt cgc tct gcc tta cgc atg ctg tct gtg tat gat ccc cac aat gca 1363 Arg Arg Ser Ala Leu Arg Met Leu Ser Val Tyr Asp Pro His Asn Ala 410 415 420 gaa cgc gtg gtt agc gcc tat ggc gga ggc gag gca cgc aca gac ttt 1411 Glu Arg Val Val Ser Ala Tyr Gly Gly Gly Glu Ala Arg Thr Asp Phe 425 430 435 tct gaa cta ctc gct gac gcg ctg ttc tgg gca ccg tcg gtt aga ttg 1459 Ser Glu Leu Leu Ala Asp Ala Leu Phe Trp Ala Pro Ser Val Arg Leu 440 445 450 gcg cag tcg cac gcc tcc caa gat gaa gat acc tgg atg tac cgc ttc 1507 Ala Gln Ser His Ala Ser Gln Asp Glu Asp Thr Trp Met Tyr Arg Phe 455 460 465 gat tac gcc cca caa tcc atg cgg aaa ctt ggc ctc ggc gcg att cac 1555 Asp Tyr Ala Pro Gln Ser Met Arg Lys Leu Gly Leu Gly Ala Ile His 470 475 480 485 tct ttc gaa ctc aac gct gtc ttt ggc gat cat gaa tct tct cgc tcc 1603 Ser Phe Glu Leu Asn Ala Val Phe Gly Asp His Glu Ser Ser Arg Ser 490 495 500 atg aac ctt gcg aaa att gcc ggc ggc atg gat cat ttg gat aaa gtc 1651 Met Asn Leu Ala Lys Ile Ala Gly Gly Met Asp His Leu Asp Lys Val 505 510 515 acc gaa ctt gtc caa gag cat tgg aag caa ttc atc tac ttc ggc agg 1699 Thr Glu Leu Val Gln Glu His Trp Lys Gln Phe Ile Tyr Phe Gly Arg 520 525 530 ccc ggt gaa gag tgg aag gct tac cgc ggc cgc agc gat acc gaa ccg 1747 Pro Gly Glu Glu Trp Lys Ala Tyr Arg Gly Arg Ser Asp Thr Glu Pro 535 540 545 ggc cgt gct aca ttt gtc atc gat atc aac tcc cga atc gcg tgg gat 1795 Gly Arg Ala Thr Phe Val Ile Asp Ile Asn Ser Arg Ile Ala Trp Asp 550 555 560 565 cca cgc cag gac aaa cgc acg gcg tgg gaa aat tac gac atg ctc gaa 1843 Pro Arg Gln Asp Lys Arg Thr Ala Trp Glu Asn Tyr Asp Met Leu Glu 570 575 580 tgg ggc aca ggc cgc cca gat cta gcc aac gaa ctt gat ttc atc gag 1891 Trp Gly Thr Gly Arg Pro Asp Leu Ala Asn Glu Leu Asp Phe Ile Glu 585 590 595 ccg gaa gag aca gaa gaa gag cag caa ttg aag tgg ctt agc ctt atg 1939 Pro Glu Glu Thr Glu Glu Glu Gln Gln Leu Lys Trp Leu Ser Leu Met 600 605 610 cag ttt ttt gga agt aga taaatgagca tctggaaacg tctgttagtg 1987 Gln Phe Phe Gly Ser Arg 615 <210> 96 <211> 619 <212> PRT <213> Corynebacterium glutamicum <400> 96 Leu Val Glu Val Phe Gly Lys Phe Leu Pro Ile Cys Val Arg Leu Val 1 5 10 15 Phe Glu Asn Pro Val His Val Arg Ile Ala Val Asp Leu Pro Ser Arg 20 25 30 Gln Ile Gly Asp Asp Pro Cys Ala His Asn Cys Ile Phe Ile Lys Ala 35 40 45 Leu Pro Val Thr Cys Phe Val Pro Ser Leu Gly Gly Met Asn Ser Ser 50 55 60 Thr Trp Thr Pro Arg Tyr Ser Pro His Met Ala Asn Ser Ala Ala Gly 65 70 75 80 Ser Val Leu Ala Asp Glu Met Val Val Pro Thr Ser Thr Gly Phe Val 85 90 95 Lys Gly Val Lys Gly Pro Gly Leu Lys Thr Trp Arg Gly Ile Pro Tyr 100 105 110 Gly Arg Asn Thr Gly Gly Lys Tyr Arg Phe Arg Ala Pro Arg Pro Ala 115 120 125 Lys Lys Trp Asp Gly Val Arg Asp Cys Ser Met Phe Gly Glu Val Ala 130 135 140 Ser Gln Pro Thr Tyr Ser Trp Thr Asp Lys Ile Arg Gly Ser Glu Asp 145 150 155 160 Cys Leu Asn Leu Asp Val Val Arg Pro Asp Ser Glu Glu Lys Leu Pro 165 170 175 Val Val Val Tyr Leu His Gly Gly Ser Phe Ile Met Gly Ser Ser Ser 180 185 190 Glu Lys Ala Leu Arg Gly Tyr Asn Leu Val Thr Asn Met Asn Val Val 195 200 205 Tyr Val Ser Val Asn Phe Arg Leu Gly Ala Leu Gly Tyr Leu Asp Leu 210 215 220 Arg Ser Val Gly Glu Asp Cys Val Ala Asn Pro Ala Leu His Asp Gln 225 230 235 240 Leu Leu Ala Leu Gln Trp Val Ser Arg Asn Ile Glu Ala Phe Gly Gly 245 250 255 Asp Pro Asp Asn Val Thr Leu Met Gly Glu Ser Ala Gly Ala Ala Ala 260 265 270 Val Val Ala Leu Met Cys Val Pro Ala Ala Gly Gly Leu Phe His Arg 275 280 285 Ala Ile Ala Gln Ser Ala Pro Val Ile Ser Val His Ser Ser Thr Gln 290 295 300 Ala Lys Phe Trp Ala Arg Glu Leu Ile Tyr Arg Met Ala Leu Pro Arg 305 310 315 320 Glu Thr Thr Leu Asp Glu Leu Arg Gln Glu Ser Ala Asp Asp Leu Val 325 330 335 Arg Ala Gly Gln Ser Met Met Trp Arg Ser Gly Glu Leu Leu Gln Leu 340 345 350 Asn Ser Cys Tyr Gly Pro Thr Val Asp Gly Ser Leu Leu Pro Glu His 355 360 365 Pro Leu Thr Met Phe Glu Gln Gly Arg Gln His Arg Ile Pro Phe Met 370 375 380 Ile Gly Thr Asn Asn Gly Glu Thr Ser Phe Ser Lys Ala Phe Tyr Leu 385 390 395 400 Arg Ser Ser Ala Arg Arg Arg Ser Ala Leu Arg Met Leu Ser Val Tyr 405 410 415 Asp Pro His Asn Ala Glu Arg Val Val Ser Ala Tyr Gly Gly Gly Glu 420 425 430 Ala Arg Thr Asp Phe Ser Glu Leu Leu Ala Asp Ala Leu Phe Trp Ala 435 440 445 Pro Ser Val Arg Leu Ala Gln Ser His Ala Ser Gln Asp Glu Asp Thr 450 455 460 Trp Met Tyr Arg Phe Asp Tyr Ala Pro Gln Ser Met Arg Lys Leu Gly 465 470 475 480 Leu Gly Ala Ile His Ser Phe Glu Leu Asn Ala Val Phe Gly Asp His 485 490 495 Glu Ser Ser Arg Ser Met Asn Leu Ala Lys Ile Ala Gly Gly Met Asp 500 505 510 His Leu Asp Lys Val Thr Glu Leu Val Gln Glu His Trp Lys Gln Phe 515 520 525 Ile Tyr Phe Gly Arg Pro Gly Glu Glu Trp Lys Ala Tyr Arg Gly Arg 530 535 540 Ser Asp Thr Glu Pro Gly Arg Ala Thr Phe Val Ile Asp Ile Asn Ser 545 550 555 560 Arg Ile Ala Trp Asp Pro Arg Gln Asp Lys Arg Thr Ala Trp Glu Asn 565 570 575 Tyr Asp Met Leu Glu Trp Gly Thr Gly Arg Pro Asp Leu Ala Asn Glu 580 585 590 Leu Asp Phe Ile Glu Pro Glu Glu Thr Glu Glu Glu Gln Gln Leu Lys 595 600 605 Trp Leu Ser Leu Met Gln Phe Phe Gly Ser Arg 610 615 <210> 97 <211> 892 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (862) <223> RXA02120 <400> 97 ttggccactt gtttgagtag gctggcgggc aggtgcttga aatactctga ttagttccaa 60 gcaaattagc acaacttcac actttattta ggagcatgtt atg tct gac ctg aag 115 Met Ser Asp Leu Lys 1 5 tca ctt gcc acg aaa ttt gct agc gat cat gaa tcc gga aag ctg ctg 163 Ser Leu Ala Thr Lys Phe Ala Ser Asp His Glu Ser Gly Lys Leu Leu 10 15 20 gtc ctg cct acc gtc tgg gat acc tgg agc gcg ggg ctc gta gaa gaa 211 Val Leu Pro Thr Val Trp Asp Thr Trp Ser Ala Gly Leu Val Glu Glu 25 30 35 gca gga ttt agt ggc ctg acc att ggt agc cac cca gtc gcg gat gcg 259 Ala Gly Phe Ser Gly Leu Thr Ile Gly Ser His Pro Val Ala Asp Ala 40 45 50 aca gga agc tcc gat ggt gaa aac atg aat ttt gca gat tat atg gcg 307 Thr Gly Ser Ser Asp Gly Glu Asn Met Asn Phe Ala Asp Tyr Met Ala 55 60 65 gtg gtc aag aag atc acc tcg gcg gta tcc atc ccc gta agc gtt gat 355 Val Val Lys Lys Ile Thr Ser Ala Val Ser Ile Pro Val Ser Val Asp 70 75 80 85 gtg gaa tcc ggt tat ggt ctc tcg cct gcg gat ttg atc gca cag att 403 Val Glu Ser Gly Tyr Gly Leu Ser Pro Ala Asp Leu Ile Ala Gln Ile 90 95 100 ttg gaa gct ggc gca gtg ggc atc aat gtg gaa gat gtt gtg cac agc 451 Leu Glu Ala Gly Ala Val Gly Ile Asn Val Glu Asp Val Val His Ser 105 110 115 gag ggt aag cgt gtt cgt gag gcg cag gag cac gct gat tac atc gct 499 Glu Gly Lys Arg Val Arg Glu Ala Gln Glu His Ala Asp Tyr Ile Ala 120 125 130 gcg gca cgt caa gct gcc gat gtg gca ggt gta gat gtg gtg atc aac 547 Ala Ala Arg Gln Ala Ala Asp Val Ala Gly Val Asp Val Val Ile Asn 135 140 145 ggt cgc acg gat gcc gtc aaa ctt ggt gca gac gtt ttt gaa gat ccg 595 Gly Arg Thr Asp Ala Val Lys Leu Gly Ala Asp Val Phe Glu Asp Pro 150 155 160 165 atg gtg gag gcc atc aag cgc atc aag ctc atg gaa cag gca ggc gcg 643 Met Val Glu Ala Ile Lys Arg Ile Lys Leu Met Glu Gln Ala Gly Ala 170 175 180 cgt tcg gtg tac ccc gtg ggt ctg agc acc gcc gag cag gtt gag cgc 691 Arg Ser Val Tyr Pro Val Gly Leu Ser Thr Ala Glu Gln Val Glu Arg 185 190 195 ctg gtg gac gct gtg tca gtg ccg gtc aac atc acc gcg cac ccg gtt 739 Leu Val Asp Ala Val Ser Val Pro Val Asn Ile Thr Ala His Pro Val 200 205 210 gat ggg cac ggc gca ggc gat ctg gcc acc ctc gca ggc ctt ggc gtg 787 Asp Gly His Gly Ala Gly Asp Leu Ala Thr Leu Ala Gly Leu Gly Val 215 220 225 cgc cgc gtg acc ttc ggt ccg ctc tgg caa aaa tgg ctg gct gcc acc 835 Arg Arg Val Thr Phe Gly Pro Leu Trp Gln Lys Trp Leu Ala Ala Thr 230 235 240 245 tcg gcg cag cag ctt aag ggc tgg gct taaattgctt gtcgacgcct 882 Ser Ala Gln Gln Leu Lys Gly Trp Ala 250 agtgccacaa 892 <210> 98 <211> 254 <212> PRT <213> Corynebacterium glutamicum <400> 98 Met Ser Asp Leu Lys Ser Leu Ala Thr Lys Phe Ala Ser Asp His Glu 1 5 10 15 Ser Gly Lys Leu Leu Val Leu Pro Thr Val Trp Asp Thr Trp Ser Ala 20 25 30 Gly Leu Val Glu Glu Ala Gly Phe Ser Gly Leu Thr Ile Gly Ser His 35 40 45 Pro Val Ala Asp Ala Thr Gly Ser Ser Asp Gly Glu Asn Met Asn Phe 50 55 60 Ala Asp Tyr Met Ala Val Val Lys Lys Ile Thr Ser Ala Val Ser Ile 65 70 75 80 Pro Val Ser Val Asp Val Glu Ser Gly Tyr Gly Leu Ser Pro Ala Asp 85 90 95 Leu Ile Ala Gln Ile Leu Glu Ala Gly Ala Val Gly Ile Asn Val Glu 100 105 110 Asp Val Val His Ser Glu Gly Lys Arg Val Arg Glu Ala Gln Glu His 115 120 125 Ala Asp Tyr Ile Ala Ala Ala Arg Gln Ala Ala Asp Val Ala Gly Val 130 135 140 Asp Val Val Ile Asn Gly Arg Thr Asp Ala Val Lys Leu Gly Ala Asp 145 150 155 160 Val Phe Glu Asp Pro Met Val Glu Ala Ile Lys Arg Ile Lys Leu Met 165 170 175 Glu Gln Ala Gly Ala Arg Ser Val Tyr Pro Val Gly Leu Ser Thr Ala 180 185 190 Glu Gln Val Glu Arg Leu Val Asp Ala Val Ser Val Pro Val Asn Ile 195 200 205 Thr Ala His Pro Val Asp Gly His Gly Ala Gly Asp Leu Ala Thr Leu 210 215 220 Ala Gly Leu Gly Val Arg Arg Val Thr Phe Gly Pro Leu Trp Gln Lys 225 230 235 240 Trp Leu Ala Ala Thr Ser Ala Gln Gln Leu Lys Gly Trp Ala 245 250 <210> 99 <211> 1219 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1189) <223> RXA02448 <400> 99 agcattcacc ggccagatct tcaagaaggc ttaaaccccc tccgatctct ttggctagca 60 cctaacaaac caccacacct tttagaaagt gattctcgct gtg aac aac tca ctc 115 Val Asn Asn Ser Leu 1 5 gca ttc aac cac gac acc ctc cca cag aaa gtc atg ttt gga tat ggc 163 Ala Phe Asn His Asp Thr Leu Pro Gln Lys Val Met Phe Gly Tyr Gly 10 15 20 aag tcc agt gca ttc tta aag cag gaa gtt gaa cgc cgc ggc tca gcc 211 Lys Ser Ser Ala Phe Leu Lys Gln Glu Val Glu Arg Arg Gly Ser Ala 25 30 35 aag gtc atg gtc att gcg ggt gaa cga gaa atg tcg atc gcc cat aag 259 Lys Val Met Val Ile Ala Gly Glu Arg Glu Met Ser Ile Ala His Lys 40 45 50 gtg gcc tca gaa att gag gtg gcg atc tgg cac gac gaa gtt gtc atg 307 Val Ala Ser Glu Ile Glu Val Ala Ile Trp His Asp Glu Val Val Met 55 60 65 cac gtg ccc atc gaa gta gcc gaa cgt gcg cgt gca gtg gca acc gac 355 His Val Pro Ile Glu Val Ala Glu Arg Ala Arg Ala Val Ala Thr Asp 70 75 80 85 aat gag att gat ctg ctg gtg tgt gtt ggc ggc gga tcc acc ata ggt 403 Asn Glu Ile Asp Leu Leu Val Cys Val Gly Gly Gly Ser Thr Ile Gly 90 95 100 ttg gcc aaa gca att gcc atg acc act gcc ctg ccc atc gtc gcg atc 451 Leu Ala Lys Ala Ile Ala Met Thr Thr Ala Leu Pro Ile Val Ala Ile 105 110 115 ccc acc acc tac gca gga tcg gaa gca acc aac gtg tgg ggt ctg acg 499 Pro Thr Thr Tyr Ala Gly Ser Glu Ala Thr Asn Val Trp Gly Leu Thr 120 125 130 gaa gca gcg cgc aaa aca acc ggt gtt gat ctg aag gtg ctc ccc gaa 547 Glu Ala Ala Arg Lys Thr Thr Gly Val Asp Leu Lys Val Leu Pro Glu 135 140 145 aca gtc att tac gat tcc gaa ctc acc atg tcg ctt cca gtg gag atg 595 Thr Val Ile Tyr Asp Ser Glu Leu Thr Met Ser Leu Pro Val Glu Met 150 155 160 165 tcc gtg gca tcc gga ctc aac ggc ctg gcg cac tgc att gat tct ttg 643 Ser Val Ala Ser Gly Leu Asn Gly Leu Ala His Cys Ile Asp Ser Leu 170 175 180 tgg gga ccc aac gcc gat ccc atc aac gca gtg ctt gca gcc gaa gga 691 Trp Gly Pro Asn Ala Asp Pro Ile Asn Ala Val Leu Ala Ala Glu Gly 185 190 195 atc cgc gca ctc aac cag gga ctg ccg aaa att gtt gcg aac ccg cac 739 Ile Arg Ala Leu Asn Gln Gly Leu Pro Lys Ile Val Ala Asn Pro His 200 205 210 agc atc gaa gga cgc gac gaa gcc ctc tac ggc gcc tac ctc gca gca 787 Ser Ile Glu Gly Arg Asp Glu Ala Leu Tyr Gly Ala Tyr Leu Ala Ala 215 220 225 gta tcc ttc gcc tcc gca ggc tcc gga cta cac cac aaa atc tgc cac 835 Val Ser Phe Ala Ser Ala Gly Ser Gly Leu His His Lys Ile Cys His 230 235 240 245 acc ttg gga ggc acc ttc aac ctc ccc cac gcc caa acc cac gca acc 883 Thr Leu Gly Gly Thr Phe Asn Leu Pro His Ala Gln Thr His Ala Thr 250 255 260 gtg ctg ccg tat gtt ttg gca ttc aac gca ggc gac gca cca gaa gct 931 Val Leu Pro Tyr Val Leu Ala Phe Asn Ala Gly Asp Ala Pro Glu Ala 265 270 275 gaa cgc cgc gca gcc gca gcc ttt gga act gac acc gca cta gaa ggc 979 Glu Arg Arg Ala Ala Ala Ala Phe Gly Thr Asp Thr Ala Leu Glu Gly 280 285 290 ctg caa cgc ctc cgc ttg tca gtc aac gca ccg aaa cga ctt tcc gac 1027 Leu Gln Arg Leu Arg Leu Ser Val Asn Ala Pro Lys Arg Leu Ser Asp 295 300 305 tac ggc ttc gag gct tca gga att gct gag gca gtg gac gtc acg ttg 1075 Tyr Gly Phe Glu Ala Ser Gly Ile Ala Glu Ala Val Asp Val Thr Leu 310 315 320 325 gag aaa gtt ccc gcc aac aat cct cgc cca gtg acc cgg gaa aac ctc 1123 Glu Lys Val Pro Ala Asn Asn Pro Arg Pro Val Thr Arg Glu Asn Leu 330 335 340 agc aga ttg ctc gaa gca gca ctc aac ggt gag gat ccg gca gtt ctt 1171 Ser Arg Leu Leu Glu Ala Ala Leu Asn Gly Glu Asp Pro Ala Val Leu 345 350 355 agc gca gta ctc agt aac taacccggcg atttttcaag gagaaaatca 1219 Ser Ala Val Leu Ser Asn 360 <210> 100 <211> 363 <212> PRT <213> Corynebacterium glutamicum <400> 100 Val Asn Asn Ser Leu Ala Phe Asn His Asp Thr Leu Pro Gln Lys Val 1 5 10 15 Met Phe Gly Tyr Gly Lys Ser Ser Ala Phe Leu Lys Gln Glu Val Glu 20 25 30 Arg Arg Gly Ser Ala Lys Val Met Val Ile Ala Gly Glu Arg Glu Met 35 40 45 Ser Ile Ala His Lys Val Ala Ser Glu Ile Glu Val Ala Ile Trp His 50 55 60 Asp Glu Val Val Met His Val Pro Ile Glu Val Ala Glu Arg Ala Arg 65 70 75 80 Ala Val Ala Thr Asp Asn Glu Ile Asp Leu Leu Val Cys Val Gly Gly 85 90 95 Gly Ser Thr Ile Gly Leu Ala Lys Ala Ile Ala Met Thr Thr Ala Leu 100 105 110 Pro Ile Val Ala Ile Pro Thr Thr Tyr Ala Gly Ser Glu Ala Thr Asn 115 120 125 Val Trp Gly Leu Thr Glu Ala Ala Arg Lys Thr Thr Gly Val Asp Leu 130 135 140 Lys Val Leu Pro Glu Thr Val Ile Tyr Asp Ser Glu Leu Thr Met Ser 145 150 155 160 Leu Pro Val Glu Met Ser Val Ala Ser Gly Leu Asn Gly Leu Ala His 165 170 175 Cys Ile Asp Ser Leu Trp Gly Pro Asn Ala Asp Pro Ile Asn Ala Val 180 185 190 Leu Ala Ala Glu Gly Ile Arg Ala Leu Asn Gln Gly Leu Pro Lys Ile 195 200 205 Val Ala Asn Pro His Ser Ile Glu Gly Arg Asp Glu Ala Leu Tyr Gly 210 215 220 Ala Tyr Leu Ala Ala Val Ser Phe Ala Ser Ala Gly Ser Gly Leu His 225 230 235 240 His Lys Ile Cys His Thr Leu Gly Gly Thr Phe Asn Leu Pro His Ala 245 250 255 Gln Thr His Ala Thr Val Leu Pro Tyr Val Leu Ala Phe Asn Ala Gly 260 265 270 Asp Ala Pro Glu Ala Glu Arg Arg Ala Ala Ala Ala Phe Gly Thr Asp 275 280 285 Thr Ala Leu Glu Gly Leu Gln Arg Leu Arg Leu Ser Val Asn Ala Pro 290 295 300 Lys Arg Leu Ser Asp Tyr Gly Phe Glu Ala Ser Gly Ile Ala Glu Ala 305 310 315 320 Val Asp Val Thr Leu Glu Lys Val Pro Ala Asn Asn Pro Arg Pro Val 325 330 335 Thr Arg Glu Asn Leu Ser Arg Leu Leu Glu Ala Ala Leu Asn Gly Glu 340 345 350 Asp Pro Ala Val Leu Ser Ala Val Leu Ser Asn 355 360 <210> 101 <211> 1033 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <101> (101) .. (1003) <223> RXA02449 <400> 101 acctcagcag attgctcgaa gcagcactca acggtgagga tccggcagtt cttagcgcag 60 tactcagtaa ctaacccggc gatttttcaa ggagaaaatc atg aca acc acc acc 115 Met Thr Thr Thr Thr 1 5 gca gac cac aac atc agc gcg cag caa aag gct gtt gaa gaa aat ctg 163 Ala Asp His Asn Ile Ser Ala Gln Gln Lys Ala Val Glu Glu Asn Leu 10 15 20 gtg aac cgt gtc ctc caa tct ttt gat gcg tgt gaa aac ccg cgc ctc 211 Val Asn Arg Val Leu Gln Ser Phe Asp Ala Cys Glu Asn Pro Arg Leu 25 30 35 aag cag ctg atg gaa tcg ctg gtt gtg cat ctg cac gat ttc atc cgg 259 Lys Gln Leu Met Glu Ser Leu Val Val His Leu His Asp Phe Ile Arg 40 45 50 gat gtg cgc ctc acc gag gat gag tgg aat tac gcc att gat ttc ctc 307 Asp Val Arg Leu Thr Glu Asp Glu Trp Asn Tyr Ala Ile Asp Phe Leu 55 60 65 aca gcg gtt ggc cac att act gat gac aag cgc caa gag ttt gtg ttg 355 Thr Ala Val Gly His Ile Thr Asp Asp Lys Arg Gln Glu Phe Val Leu 70 75 80 85 ctt tcc gat acc ttg ggc gcc tcg atg cag acc atc gcg gtg aac aac 403 Leu Ser Asp Thr Leu Gly Ala Ser Met Gln Thr Ile Ala Val Asn Asn 90 95 100 gaa gcg tat gaa aac tca acg gaa gct aca gtc ttt ggt cca ttc ttc 451 Glu Ala Tyr Glu Asn Ser Thr Glu Ala Thr Val Phe Gly Pro Phe Phe 105 110 115 ctc gat gac gct cct gag gtt gag ctg ggt gga gat atc gcc ggc ggc 499 Leu Asp Asp Ala Pro Glu Val Glu Leu Gly Gly Asp Ile Ala Gly Gly 120 125 130 gcc cag ggg cag gca gcg tgg att gaa gga acc gtc acc gac act gaa 547 Ala Gln Gly Gln Ala Ala Trp Ile Glu Gly Thr Val Thr Asp Thr Glu 135 140 145 ggc aat ccc gtt ccg aat gct cgt atc gag gtg tgg gag tgc gat gaa 595 Gly Asn Pro Val Pro Asn Ala Arg Ile Glu Val Trp Glu Cys Asp Glu 150 155 160 165 gat gga ctc tac gat gtc caa tat gcc gat gag cgc atg gcg ggt cgc 643 Asp Gly Leu Tyr Asp Val Gln Tyr Ala Asp Glu Arg Met Ala Gly Arg 170 175 180 gcg tat atg cac acc gac gcc aat ggc gat tac cgc ttc tgg ggt ctg 691 Ala Tyr Met His Thr Asp Ala Asn Gly Asp Tyr Arg Phe Trp Gly Leu 185 190 195 act ccg gtt cct tat cca atc ccc cac gat ggc ccc gtg ggc aac atg 739 Thr Pro Val Pro Tyr Pro Ile Pro His Asp Gly Pro Val Gly Asn Met 200 205 210 ctc aag gcg gtt ggt cgt tcg ccg gtg cgc tgc gcc cac ctt cac ttc 787 Leu Lys Ala Val Gly Arg Ser Pro Val Arg Cys Ala His Leu His Phe 215 220 225 atg gtg acc gct cct gaa ttg cgc acc ttg gtc act cac att ttc gtt 835 Met Val Thr Ala Pro Glu Leu Arg Thr Leu Val Thr His Ile Phe Val 230 235 240 245 gag ggt gat ccg cag cta gaa atc ggc gat tcc gtc ttc ggg gtc aag 883 Glu Gly Asp Pro Gln Leu Glu Ile Gly Asp Ser Val Phe Gly Val Lys 250 255 260 gac tcg ttg atc aag aag ttt gag gag cag gcg cca ggc act cct acc 931 Asp Ser Leu Ile Lys Lys Phe Glu Glu Gln Ala Pro Gly Thr Pro Thr 265 270 275 cca gat ggt cgg gat ctc ggc gat cag acg tgg gcg cgc acg cgc ttc 979 Pro Asp Gly Arg Asp Leu Gly Asp Gln Thr Trp Ala Arg Thr Arg Phe 280 285 290 gac atc gtg ctc gcg ccg ggc gcg taggatttcg cttttcgacg caaccctcca 1033 Asp Ile Val Leu Ala Pro Gly Ala 295 300 <210> 102 <211> 301 <212> PRT <213> Corynebacterium glutamicum <400> 102 Met Thr Thr Thr Thr Ala Asp His Asn Ile Ser Ala Gln Gln Lys Ala 1 5 10 15 Val Glu Glu Asn Leu Val Asn Arg Val Leu Gln Ser Phe Asp Ala Cys 20 25 30 Glu Asn Pro Arg Leu Lys Gln Leu Met Glu Ser Leu Val Val His Leu 35 40 45 His Asp Phe Ile Arg Asp Val Arg Leu Thr Glu Asp Glu Trp Asn Tyr 50 55 60 Ala Ile Asp Phe Leu Thr Ala Val Gly His Ile Thr Asp Asp Lys Arg 65 70 75 80 Gln Glu Phe Val Leu Leu Ser Asp Thr Leu Gly Ala Ser Met Gln Thr 85 90 95 Ile Ala Val Asn Asn Glu Ala Tyr Glu Asn Ser Thr Glu Ala Thr Val 100 105 110 Phe Gly Pro Phe Phe Leu Asp Asp Ala Pro Glu Val Glu Leu Gly Gly 115 120 125 Asp Ile Ala Gly Gly Ala Gln Gly Gln Ala Ala Trp Ile Glu Gly Thr 130 135 140 Val Thr Asp Thr Glu Gly Asn Pro Val Pro Asn Ala Arg Ile Glu Val 145 150 155 160 Trp Glu Cys Asp Glu Asp Gly Leu Tyr Asp Val Gln Tyr Ala Asp Glu 165 170 175 Arg Met Ala Gly Arg Ala Tyr Met His Thr Asp Ala Asn Gly Asp Tyr 180 185 190 Arg Phe Trp Gly Leu Thr Pro Val Pro Tyr Pro Ile Pro His Asp Gly 195 200 205 Pro Val Gly Asn Met Leu Lys Ala Val Gly Arg Ser Pro Val Arg Cys 210 215 220 Ala His Leu His Phe Met Val Thr Ala Pro Glu Leu Arg Thr Leu Val 225 230 235 240 Thr His Ile Phe Val Glu Gly Asp Pro Gln Leu Glu Ile Gly Asp Ser 245 250 255 Val Phe Gly Val Lys Asp Ser Leu Ile Lys Lys Phe Glu Glu Gln Ala 260 265 270 Pro Gly Thr Pro Thr Pro Asp Gly Arg Asp Leu Gly Asp Gln Thr Trp 275 280 285 Ala Arg Thr Arg Phe Asp Ile Val Leu Ala Pro Gly Ala 290 295 300 <210> 103 <211> 940 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (910) <223> RXA02474 <400> 103 ataatcttca tcacatggtg tgtgcagaaa tatttttgct ggtctattgt ggcgaccgag 60 ggcctttgaa ggttcgacaa actgtataag gccttgaatc ttg aga att tat ttt 115 Leu Arg Ile Tyr Phe 1 5 gag gaa gca aga gga agt gtc atg agc aaa gtt gca atg gtt acc ggt 163 Glu Glu Ala Arg Gly Ser Val Met Ser Lys Val Ala Met Val Thr Gly 10 15 20 ggt gca caa ggc atc ggt cgt gga att tca gag aag ctg gca gca gat 211 Gly Ala Gln Gly Ile Gly Arg Gly Ile Ser Glu Lys Leu Ala Ala Asp 25 30 35 ggt ttc gat att gcc gta gcc gac ctg cca caa cag gaa gaa caa gct 259 Gly Phe Asp Ile Ala Val Ala Asp Leu Pro Gln Gln Glu Glu Gln Ala 40 45 50 gca gag acc atc aag ttg att gaa gct gca ggt caa aag gct gta ttc 307 Ala Glu Thr Ile Lys Leu Ile Glu Ala Ala Gly Gln Lys Ala Val Phe 55 60 65 gtt gga tta gat gtc acc gat aag gct aat ttc gac agt gca att gat 355 Val Gly Leu Asp Val Thr Asp Lys Ala Asn Phe Asp Ser Ala Ile Asp 70 75 80 85 gag gca gca gag aaa ctt ggc ggc ttc gat gtg cta gta aac aac gcc 403 Glu Ala Ala Glu Lys Leu Gly Gly Phe Asp Val Leu Val Asn Asn Ala 90 95 100 ggc atc gca caa att aag cca ctt ctg gaa gtc acc gaa gaa gac cta 451 Gly Ile Ala Gln Ile Lys Pro Leu Leu Glu Val Thr Glu Glu Asp Leu 105 110 115 aag cag atc tac tcc gtg aac gtt ttt agc gta ttt ttt ggt att caa 499 Lys Gln Ile Tyr Ser Val Asn Val Phe Ser Val Phe Phe Gly Ile Gln 120 125 130 gca gca tcc cga aag ttc gat gag ctt ggc gta aaa ggc aag atc atc 547 Ala Ala Ser Arg Lys Phe Asp Glu Leu Gly Val Lys Gly Lys Ile Ile 135 140 145 aac gct gca tca atc gct gct atc caa ggt ttc cca atc ttg agc gcc 595 Asn Ala Ala Ser Ile Ala Ala Ile Gln Gly Phe Pro Ile Leu Ser Ala 150 155 160 165 tac tcc acc acc aaa ttc gcg gtt cgt ggc ctc acc cag gct gct gcg 643 Tyr Ser Thr Thr Lys Phe Ala Val Arg Gly Leu Thr Gln Ala Ala Ala 170 175 180 caa gaa ctc gca ccc aag ggt cac acc gtg aat gcc tac gca cct ggc 691 Gln Glu Leu Ala Pro Lys Gly His Thr Val Asn Ala Tyr Ala Pro Gly 185 190 195 atc gtg ggc acc gga atg tgg gag caa atc gat gcc gag ctt tcc aag 739 Ile Val Gly Thr Gly Met Trp Glu Gln Ile Asp Ala Glu Leu Ser Lys 200 205 210 atc aac ggc aag cca atc ggt gag aac ttc aag gag tac tcc tcc tca 787 Ile Asn Gly Lys Pro Ile Gly Glu Asn Phe Lys Glu Tyr Ser Ser Ser 215 220 225 atc gca ttg ggc cga cca tca gta cct gag gat gta gcc ggt ctg gtt 835 Ile Ala Leu Gly Arg Pro Ser Val Pro Glu Asp Val Ala Gly Leu Val 230 235 240 245 tcg ttc ctg gct tct gaa aac tcc aac tac atc acc gga cag gtc atg 883 Ser Phe Leu Ala Ser Glu Asn Ser Asn Tyr Ile Thr Gly Gln Val Met 250 255 260 ctt gtc gac ggc ggc atg ctc tac aac taggggttgc tttcccgcac 930 Leu Val Asp Gly Gly Met Leu Tyr Asn 265 270 tcaacgcagt 940 <210> 104 <211> 270 <212> PRT <213> Corynebacterium glutamicum <400> 104 Leu Arg Ile Tyr Phe Glu Glu Ala Arg Gly Ser Val Met Ser Lys Val 1 5 10 15 Ala Met Val Thr Gly Gly Ala Gln Gly Ile Gly Arg Gly Ile Ser Glu 20 25 30 Lys Leu Ala Ala Asp Gly Phe Asp Ile Ala Val Ala Asp Leu Pro Gln 35 40 45 Gln Glu Glu Gln Ala Ala Glu Thr Ile Lys Leu Ile Glu Ala Ala Gly 50 55 60 Gln Lys Ala Val Phe Val Gly Leu Asp Val Thr Asp Lys Ala Asn Phe 65 70 75 80 Asp Ser Ala Ile Asp Glu Ala Ala Glu Lys Leu Gly Gly Phe Asp Val 85 90 95 Leu Val Asn Asn Ala Gly Ile Ala Gln Ile Lys Pro Leu Leu Glu Val 100 105 110 Thr Glu Glu Asp Leu Lys Gln Ile Tyr Ser Val Asn Val Phe Ser Val 115 120 125 Phe Phe Gly Ile Gln Ala Ala Ser Arg Lys Phe Asp Glu Leu Gly Val 130 135 140 Lys Gly Lys Ile Ile Asn Ala Ala Ser Ile Ala Ala Ile Gln Gly Phe 145 150 155 160 Pro Ile Leu Ser Ala Tyr Ser Thr Thr Lys Phe Ala Val Arg Gly Leu 165 170 175 Thr Gln Ala Ala Ala Gln Glu Leu Ala Pro Lys Gly His Thr Val Asn 180 185 190 Ala Tyr Ala Pro Gly Ile Val Gly Thr Gly Met Trp Glu Gln Ile Asp 195 200 205 Ala Glu Leu Ser Lys Ile Asn Gly Lys Pro Ile Gly Glu Asn Phe Lys 210 215 220 Glu Tyr Ser Ser Ser Ile Ala Leu Gly Arg Pro Ser Val Pro Glu Asp 225 230 235 240 Val Ala Gly Leu Val Ser Phe Leu Ala Ser Glu Asn Ser Asn Tyr Ile 245 250 255 Thr Gly Gln Val Met Leu Val Asp Gly Gly Met Leu Tyr Asn 260 265 270 <210> 105 <211> 1381 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1351) <223> RXA02535 <400> 105 aattttcttt tatccatcag tcattttaag cttccgcccc tcggcaaggt gacgaaaatt 60 ttgttattct ctacggtttg agggcttctc gcgggaagga ttg ggt gag gag gga 115 Leu Gly Glu Glu Gly 1 5 ggc gtc gaa aag caa gaa acg cct gac acg cgt agc cgt ggc tgc gca 163 Gly Val Glu Lys Gln Glu Thr Pro Asp Thr Arg Ser Arg Gly Cys Ala 10 15 20 cct ttt acc ttg att agg tgc aca ata ggg agt gtg aca cct cgc cct 211 Pro Phe Thr Leu Ile Arg Cys Thr Ile Gly Ser Val Thr Pro Arg Pro 25 30 35 gtt tct tct gtt gcg cga ctc gtt gag gat aac gcg caa gat ttt ctt 259 Val Ser Ser Val Ala Arg Leu Val Glu Asp Asn Ala Gln Asp Phe Leu 40 45 50 cgc gcc gtt cag gcg aaa ctt tta acg ctc gcg cca cag gct cgt ggg 307 Arg Ala Val Gln Ala Lys Leu Leu Thr Leu Ala Pro Gln Ala Arg Gly 55 60 65 cat ttt ccc act gcg gat gat gcg act cat atc agc atc gcc gaa atg 355 His Phe Pro Thr Ala Asp Asp Ala Thr His Ile Ser Ile Ala Glu Met 70 75 80 85 gtg agt gcg ctg ttg gaa ggc act ggt gag gaa ggg aaa gta gac gac 403 Val Ser Ala Leu Leu Glu Gly Thr Gly Glu Glu Gly Lys Val Asp Asp 90 95 100 aaa aca ctg gag ttc ttt aaa gaa gcg gcg tta gat gcg cgt cga ttt 451 Lys Thr Leu Glu Phe Phe Lys Glu Ala Ala Leu Asp Ala Arg Arg Phe 105 110 115 ggc ctg acc cca gaa atg cac agc gct ttg ggt gag gcc gtg cgc agc 499 Gly Leu Thr Pro Glu Met His Ser Ala Leu Gly Glu Ala Val Arg Ser 120 125 130 gaa cta ttg tca tta tgc gag gat ctt ccc ttt gaa aat gtg ctg ttt 547 Glu Leu Leu Ser Leu Cys Glu Asp Leu Pro Phe Glu Asn Val Leu Phe 135 140 145 gcc gag cgt gcg att gct gca acc acg gct gtt tcc gtc gag gcg gtt 595 Ala Glu Arg Ala Ile Ala Ala Thr Thr Ala Val Ser Val Glu Ala Val 150 155 160 165 cgt gaa gct gat gag gca cac att cct gcc tca tat cag gca gaa att 643 Arg Glu Ala Asp Glu Ala His Ile Pro Ala Ser Tyr Gln Ala Glu Ile 170 175 180 gta gaa gtt gaa aag cgc agc cgt agg ttc acc gtc gtg cgc atg caa 691 Val Glu Val Glu Lys Arg Ser Arg Arg Phe Thr Val Val Arg Met Gln 185 190 195 gct gaa acg caa ctg ccc tac cta ccg gga caa tat ctt gca gca act 739 Ala Glu Thr Gln Leu Pro Tyr Leu Pro Gly Gln Tyr Leu Ala Ala Thr 200 205 210 gcg gat ttt ctg ccc aac aca tgg cgc tac ctg tgc cct tcg atc ccc 787 Ala Asp Phe Leu Pro Asn Thr Trp Arg Tyr Leu Cys Pro Ser Ile Pro 215 220 225 act aac gaa tgg ggg cag gtg gag ttt cac atc caa tca gac gca gat 835 Thr Asn Glu Trp Gly Gln Val Glu Phe His Ile Gln Ser Asp Ala Asp 230 235 240 245 gat atc gct gga ctt tta gcc acc aca cgc ctt ggc gat aaa tgg cga 883 Asp Ile Ala Gly Leu Leu Ala Thr Thr Arg Leu Gly Asp Lys Trp Arg 250 255 260 ctt ggc ccc ggt cgt gga gat ttc gga caa agc aaa att agt tcc ggc 931 Leu Gly Pro Gly Arg Gly Asp Phe Gly Gln Ser Lys Ile Ser Ser Gly 265 270 275 aat gat ttg tta ttt att gca cat gga acg ggc cta gct cct ctt cgc 979 Asn Asp Leu Leu Phe Ile Ala His Gly Thr Gly Leu Ala Pro Leu Arg 280 285 290 gct tac atg ttt gag ctg atg aac caa gca gca cct cca cgt ttg cac 1027 Ala Tyr Met Phe Glu Leu Met Asn Gln Ala Ala Pro Pro Arg Leu His 295 300 305 ttt ttc gtc ggt gcc gac tac ccc ggt gag ctg tat gaa ctc acc ggc 1075 Phe Phe Val Gly Ala Asp Tyr Pro Gly Glu Leu Tyr Glu Leu Thr Gly 310 315 320 325 atg tgg aat ttt gct gca gcc agc cca tgg ctt tct gtc gta ccg gtg 1123 Met Trp Asn Phe Ala Ala Ala Ser Pro Trp Leu Ser Val Val Pro Val 330 335 340 tca acg cat gac aag gat gcg tgg tgg gtt caa gcc acc gaa gca tcg 1171 Ser Thr His Asp Lys Asp Ala Trp Trp Val Gln Ala Thr Glu Ala Ser 345 350 355 cag cca ccg agg ggt ttg cat ttg cat caa acg ggt tcg atg gcg aag 1219 Gln Pro Pro Arg Gly Leu His Leu His Gln Thr Gly Ser Met Ala Lys 360 365 370 atc gtc acc gaa gca ggt gct tgg gcc gac cgc gac gtg tta att gct 1267 Ile Val Thr Glu Ala Gly Ala Trp Ala Asp Arg Asp Val Leu Ile Ala 375 380 385 ggc cct gaa tcg tgg gct cgg gat gtt cgg cgc gcg atg att agg cgt 1315 Gly Pro Glu Ser Trp Ala Arg Asp Val Arg Arg Ala Met Ile Arg Arg 390 395 400 405 gga act ccg gcc cag cag att gag att tta ggg ttc taggttgcct 1361 Gly Thr Pro Ala Gln Gln Ile Glu Ile Leu Gly Phe 410 415 caccgggctg accaatcaga 1381 <210> 106 <211> 417 <212> PRT <213> Corynebacterium glutamicum <400> 106 Leu Gly Glu Glu Gly Gly Val Glu Lys Gln Glu Thr Pro Asp Thr Arg 1 5 10 15 Ser Arg Gly Cys Ala Pro Phe Thr Leu Ile Arg Cys Thr Ile Gly Ser 20 25 30 Val Thr Pro Arg Pro Val Ser Ser Val Ala Arg Leu Val Glu Asp Asn 35 40 45 Ala Gln Asp Phe Leu Arg Ala Val Gln Ala Lys Leu Leu Thr Leu Ala 50 55 60 Pro Gln Ala Arg Gly His Phe Pro Thr Ala Asp Asp Ala Thr His Ile 65 70 75 80 Ser Ile Ala Glu Met Val Ser Ala Leu Leu Glu Gly Thr Gly Glu Glu 85 90 95 Gly Lys Val Asp Asp Lys Thr Leu Glu Phe Phe Lys Glu Ala Ala Leu 100 105 110 Asp Ala Arg Arg Phe Gly Leu Thr Pro Glu Met His Ser Ala Leu Gly 115 120 125 Glu Ala Val Arg Ser Glu Leu Leu Ser Leu Cys Glu Asp Leu Pro Phe 130 135 140 Glu Asn Val Leu Phe Ala Glu Arg Ala Ile Ala Ala Thr Thr Ala Val 145 150 155 160 Ser Val Glu Ala Val Arg Glu Ala Asp Glu Ala His Ile Pro Ala Ser 165 170 175 Tyr Gln Ala Glu Ile Val Glu Val Glu Lys Arg Ser Arg Arg Phe Thr 180 185 190 Val Val Arg Met Gln Ala Glu Thr Gln Leu Pro Tyr Leu Pro Gly Gln 195 200 205 Tyr Leu Ala Ala Thr Ala Asp Phe Leu Pro Asn Thr Trp Arg Tyr Leu 210 215 220 Cys Pro Ser Ile Pro Thr Asn Glu Trp Gly Gln Val Glu Phe His Ile 225 230 235 240 Gln Ser Asp Ala Asp Asp Ile Ala Gly Leu Leu Ala Thr Thr Arg Leu 245 250 255 Gly Asp Lys Trp Arg Leu Gly Pro Gly Arg Gly Asp Phe Gly Gln Ser 260 265 270 Lys Ile Ser Ser Gly Asn Asp Leu Leu Phe Ile Ala His Gly Thr Gly 275 280 285 Leu Ala Pro Leu Arg Ala Tyr Met Phe Glu Leu Met Asn Gln Ala Ala 290 295 300 Pro Pro Arg Leu His Phe Phe Val Gly Ala Asp Tyr Pro Gly Glu Leu 305 310 315 320 Tyr Glu Leu Thr Gly Met Trp Asn Phe Ala Ala Ala Ser Pro Trp Leu 325 330 335 Ser Val Val Pro Val Ser Thr His Asp Lys Asp Ala Trp Trp Val Gln 340 345 350 Ala Thr Glu Ala Ser Gln Pro Pro Arg Gly Leu His Leu His Gln Thr 355 360 365 Gly Ser Met Ala Lys Ile Val Thr Glu Ala Gly Ala Trp Ala Asp Arg 370 375 380 Asp Val Leu Ile Ala Gly Pro Glu Ser Trp Ala Arg Asp Val Arg Arg 385 390 395 400 Ala Met Ile Arg Arg Gly Thr Pro Ala Gln Gln Ile Glu Ile Leu Gly 405 410 415 Phe <210> 107 <211> 997 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (967) <223> RXA02560 <400> 107 ttggggcaag ccagctaacg catttcttgt ggaaaccgca gacattgagg ccgcccacgc 60 ggaacttcta agagcagtgg aatgaaataa tccggtgctg atg cag ggc aac tcg 115 Met Gln Gly Asn Ser 1 5 ctt aat ctg gca gac aac agc gag aga aag aag ccc atg ccg tca cca 163 Leu Asn Leu Ala Asp Asn Ser Glu Arg Lys Lys Pro Met Pro Ser Pro 10 15 20 gga gaa ctt tta gcc gcc cgc tac gga caa cct gca acc tgg acg cca 211 Gly Glu Leu Leu Ala Ala Arg Tyr Gly Gln Pro Ala Thr Trp Thr Pro 25 30 35 ccg cag tgg aat gag acg ctt gat gtc att cac cag cat cga tca gtt 259 Pro Gln Trp Asn Glu Thr Leu Asp Val Ile His Gln His Arg Ser Val 40 45 50 cgc agg tgg ttg gat aaa ccg gtt gat gat gac acc atc cgc acc att 307 Arg Arg Trp Leu Asp Lys Pro Val Asp Asp Asp Thr Ile Arg Thr Ile 55 60 65 att tcc gcc gca caa tcg gct gga acc tct tcc aat aag cag gtc att 355 Ile Ser Ala Ala Gln Ser Ala Gly Thr Ser Ser Asn Lys Gln Val Ile 70 75 80 85 tct gtc atc gtg gtt aaa gat cct gag ctg agg aaa ggc ctc gcg ggg 403 Ser Val Ile Val Val Lys Asp Pro Glu Leu Arg Lys Gly Leu Ala Gly 90 95 100 atc act cgc cag atg ttt ccg cac ctt gag cag gtt ccc gcg gtg ctg 451 Ile Thr Arg Gln Met Phe Pro His Leu Glu Gln Val Pro Ala Val Leu 105 110 115 att tgg ttg att gat tat tcc cga atc agt gcg gtg gca gcc aga gaa 499 Ile Trp Leu Ile Asp Tyr Ser Arg Ile Ser Ala Val Ala Ala Arg Glu 120 125 130 gat ctc cca aca ggg gct ctt gat tat ctc gat gag gcc gcg ttg ggg 547 Asp Leu Pro Thr Gly Ala Leu Asp Tyr Leu Asp Glu Ala Ala Leu Gly 135 140 145 ttc ctc gac gcc gga atc gca gct caa aac gct gca att gct gcg gag 595 Phe Leu Asp Ala Gly Ile Ala Ala Gln Asn Ala Ala Ile Ala Ala Glu 150 155 160 165 tca ctt gga ttg gga acg ctc tat ttg ggt tcg gtg cgc aac gat gcg 643 Ser Leu Gly Leu Gly Thr Leu Tyr Leu Gly Ser Val Arg Asn Asp Ala 170 175 180 gaa gcc gtg cac aaa ttg ctt ggc ctt cca cct gag atc gtg cct gtc 691 Glu Ala Val His Lys Leu Leu Gly Leu Pro Pro Glu Ile Val Pro Val 185 190 195 gtg ggc ttg gaa att ggg cat gcg gat ccg tct gaa cct gcc gga att 739 Val Gly Leu Glu Ile Gly His Ala Asp Pro Ser Glu Pro Ala Gly Ile 200 205 210 aaa cct cgc ctg cca caa gaa gcc att gtt cac tgg gat acc tac acc 787 Lys Pro Arg Leu Pro Gln Glu Ala Ile Val His Trp Asp Thr Tyr Thr 215 220 225 gag aaa aac ctc gaa ctt atc gat tcc tac gac cgc gcc ctc gac act 835 Glu Lys Asn Leu Glu Leu Ile Asp Ser Tyr Asp Arg Ala Leu Asp Thr 230 235 240 245 tac tat tct cgc tac ggc cag cac cag ctc tgg tcg aag cag acg gcg 883 Tyr Tyr Ser Arg Tyr Gly Gln His Gln Leu Trp Ser Lys Gln Thr Ala 250 255 260 cat agg gcg gcg tcg aaa agc ttt tca aaa acc aac agg cag ttc ctt 931 His Arg Ala Ala Ser Lys Ser Phe Ser Lys Thr Asn Arg Gln Phe Leu 265 270 275 agg ggc gtg ttt gag cgc gcc ggg ttt ggg ctg aga taaaagcatg 977 Arg Gly Val Phe Glu Arg Ala Gly Phe Gly Leu Arg 280 285 attatggacg cctccctccc 997 <210> 108 <211> 289 <212> PRT <213> Corynebacterium glutamicum <400> 108 Met Gln Gly Asn Ser Leu Asn Leu Ala Asp Asn Ser Glu Arg Lys Lys 1 5 10 15 Pro Met Pro Ser Pro Gly Glu Leu Leu Ala Ala Arg Tyr Gly Gln Pro 20 25 30 Ala Thr Trp Thr Pro Pro Gln Trp Asn Glu Thr Leu Asp Val Ile His 35 40 45 Gln His Arg Ser Val Arg Arg Trp Leu Asp Lys Pro Val Asp Asp Asp 50 55 60 Thr Ile Arg Thr Ile Ile Ser Ala Ala Gln Ser Ala Gly Thr Ser Ser 65 70 75 80 Asn Lys Gln Val Ile Ser Val Ile Val Val Lys Asp Pro Glu Leu Arg 85 90 95 Lys Gly Leu Ala Gly Ile Thr Arg Gln Met Phe Pro His Leu Glu Gln 100 105 110 Val Pro Ala Val Leu Ile Trp Leu Ile Asp Tyr Ser Arg Ile Ser Ala 115 120 125 Val Ala Ala Arg Glu Asp Leu Pro Thr Gly Ala Leu Asp Tyr Leu Asp 130 135 140 Glu Ala Ala Leu Gly Phe Leu Asp Ala Gly Ile Ala Ala Gln Asn Ala 145 150 155 160 Ala Ile Ala Ala Glu Ser Leu Gly Leu Gly Thr Leu Tyr Leu Gly Ser 165 170 175 Val Arg Asn Asp Ala Glu Ala Val His Lys Leu Leu Gly Leu Pro Pro 180 185 190 Glu Ile Val Pro Val Val Gly Leu Glu Ile Gly His Ala Asp Pro Ser 195 200 205 Glu Pro Ala Gly Ile Lys Pro Arg Leu Pro Gln Glu Ala Ile Val His 210 215 220 Trp Asp Thr Tyr Thr Glu Lys Asn Leu Glu Leu Ile Asp Ser Tyr Asp 225 230 235 240 Arg Ala Leu Asp Thr Tyr Tyr Ser Arg Tyr Gly Gln His Gln Leu Trp 245 250 255 Ser Lys Gln Thr Ala His Arg Ala Ala Ser Lys Ser Phe Ser Lys Thr 260 265 270 Asn Arg Gln Phe Leu Arg Gly Val Phe Glu Arg Ala Gly Phe Gly Leu 275 280 285 Arg <210> 109 <211> 1453 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1423) <223> RXA02630 <400> 109 gcgggttcga aaatgtcgat gattaaacca ctaaagagct cacaggaagt gttcagacta 60 cttagagtga cgccccagcc acagggttca taatcaaatc atg aca aat caa ttc 115 Met Thr Asn Gln Phe 1 5 ccc aca aac aac ggt gag aac ccg gac cgt gca tcg gaa act cca tca 163 Pro Thr Asn Asn Gly Glu Asn Pro Asp Arg Ala Ser Glu Thr Pro Ser 10 15 20 gaa acc aac tcc ttc gaa cat gtg cgt agt tca tat ccg cag tgg ggt 211 Glu Thr Asn Ser Phe Glu His Val Arg Ser Ser Tyr Pro Gln Trp Gly 25 30 35 aac act gct tcc aat caa aac ccc tat cct ggt gcg ggc ttc ggc tct 259 Asn Thr Ala Ser Asn Gln Asn Pro Tyr Pro Gly Ala Gly Phe Gly Ser 40 45 50 gaa caa aac act caa caa gga aat gag caa caa gct cca gcc tgg acc 307 Glu Gln Asn Thr Gln Gln Gly Asn Glu Gln Gln Ala Pro Ala Trp Thr 55 60 65 agt tgg gat aat cag cct cta agc aca gat gta aag cca gct aaa gaa 355 Ser Trp Asp Asn Gln Pro Leu Ser Thr Asp Val Lys Pro Ala Lys Glu 70 75 80 85 aag cga aaa gtt ggc atc gga acg gca ctc gcg tta atg ctt gtt ggt 403 Lys Arg Lys Val Gly Ile Gly Thr Ala Leu Ala Leu Met Leu Val Gly 90 95 100 tct att gct acc ggt agc gtt gtg ggt gtt gca gca acc cag ctt ggt 451 Ser Ile Ala Thr Gly Ser Val Val Gly Val Ala Ala Thr Gln Leu Gly 105 110 115 tcg gac tct tca acc cca gtt aat gct ctt gag cag ccc agc gtg cag 499 Ser Asp Ser Ser Thr Pro Val Asn Ala Leu Glu Gln Pro Ser Val Gln 120 125 130 cgc acc act aat gct gaa cca ggt tca gcg gaa cag gtt gct gcc gca 547 Arg Thr Thr Asn Ala Glu Pro Gly Ser Ala Glu Gln Val Ala Ala Ala 135 140 145 gtt ttg cct tct gtc gtc tct att cag gcc att act agg acg tct gct 595 Val Leu Pro Ser Val Val Ser Ile Gln Ala Ile Thr Arg Thr Ser Ala 150 155 160 165 tct gag ggc tct gga tcc att att tcc tct gat ggt tac gtc atg acc 643 Ser Glu Gly Ser Gly Ser Ile Ile Ser Ser Asp Gly Tyr Val Met Thr 170 175 180 aat aat cac gtc gtg gca ggc att gaa caa tct ggt gtg tta gaa gta 691 Asn Asn His Val Val Ala Gly Ile Glu Gln Ser Gly Val Leu Glu Val 185 190 195 agt ttc tcc gat gga act aca gcg caa gct gat ttt att gct ggt gat 739 Ser Phe Ser Asp Gly Thr Thr Ala Gln Ala Asp Phe Ile Ala Gly Asp 200 205 210 cct tcc aca gat att gct gtg atc aag att agg gat gtg tcc aac ctt 787 Pro Ser Thr Asp Ile Ala Val Ile Lys Ile Arg Asp Val Ser Asn Leu 215 220 225 cca gtt atg agc ttt gga gat tcg gac gca tta ggc gtt gga caa agt 835 Pro Val Met Ser Phe Gly Asp Ser Asp Ala Leu Gly Val Gly Gln Ser 230 235 240 245 gtg atg gct gtt ggt tct cca ctg ggt ctg agc tcc act gtg acc acc 883 Val Met Ala Val Gly Ser Pro Leu Gly Leu Ser Ser Thr Val Thr Thr 250 255 260 ggt att gtg tcg gcc gtg aac cgt cct gtg cga gct tct ggt gat ggc 931 Gly Ile Val Ser Ala Val Asn Arg Pro Val Arg Ala Ser Gly Asp Gly 265 270 275 gga gag tcg tcc ctc atc gat gct atc cag acc gat gct gcg atc aac 979 Gly Glu Ser Ser Leu Ile Asp Ala Ile Gln Thr Asp Ala Ala Ile Asn 280 285 290 cct ggt aac tct ggt ggt ccg ctg gtt gat atg gat ggc aac ctc att 1027 Pro Gly Asn Ser Gly Gly Pro Leu Val Asp Met Asp Gly Asn Leu Ile 295 300 305 ggc atg aat tcg gta att gca tcg att tcg agc acc agc gat tcc gca 1075 Gly Met Asn Ser Val Ile Ala Ser Ile Ser Ser Thr Ser Asp Ser Ala 310 315 320 325 ggt tcc att ggt ctt ggt ttt tct atc cca tcc aac ttt gcc aag cgc 1123 Gly Ser Ile Gly Leu Gly Phe Ser Ile Pro Ser Asn Phe Ala Lys Arg 330 335 340 gtg gcc gat caa ttg atc agc acc ggc cag gta act cag ccg atg atc 1171 Val Ala Asp Gln Leu Ile Ser Thr Gly Gln Val Thr Gln Pro Met Ile 345 350 355 ggt gtg cag gtt ggc act gac aac tca gtg aca ggc gct gtg att gcc 1219 Gly Val Gln Val Gly Thr Asp Asn Ser Val Thr Gly Ala Val Ile Ala 360 365 370 agt gtt caa gat ggt gga ccg gcc gca gat gct gga ctt cag cca ggc 1267 Ser Val Gln Asp Gly Gly Pro Ala Ala Asp Ala Gly Leu Gln Pro Gly 375 380 385 gat atc gtg acc aag ctc aat gat cga gtt att gat agc cca gac tcc 1315 Asp Ile Val Thr Lys Leu Asn Asp Arg Val Ile Asp Ser Pro Asp Ser 390 395 400 405 ttg atc gct gct gtt cgt tcg cat gat ttt ggc gaa acc gtc act tta 1363 Leu Ile Ala Ala Val Arg Ser His Asp Phe Gly Glu Thr Val Thr Leu 410 415 420 aca att aca cag cca gat acc tcg cag agc cgg gag gta gag gtt act 1411 Thr Ile Thr Gln Pro Asp Thr Ser Gln Ser Arg Glu Val Glu Val Thr 425 430 435 ctg acg agt gag taggtttaaa agagttaatc tgcatctaat 1453 Leu Thr Ser Glu 440 <210> 110 <211> 441 <212> PRT <213> Corynebacterium glutamicum <400> 110 Met Thr Asn Gln Phe Pro Thr Asn Asn Gly Glu Asn Pro Asp Arg Ala 1 5 10 15 Ser Glu Thr Pro Ser Glu Thr Asn Ser Phe Glu His Val Arg Ser Ser 20 25 30 Tyr Pro Gln Trp Gly Asn Thr Ala Ser Asn Gln Asn Pro Tyr Pro Gly 35 40 45 Ala Gly Phe Gly Ser Glu Gln Asn Thr Gln Gln Gly Asn Glu Gln Gln 50 55 60 Ala Pro Ala Trp Thr Ser Trp Asp Asn Gln Pro Leu Ser Thr Asp Val 65 70 75 80 Lys Pro Ala Lys Glu Lys Arg Lys Val Gly Ile Gly Thr Ala Leu Ala 85 90 95 Leu Met Leu Val Gly Ser Ile Ala Thr Gly Ser Val Val Gly Val Ala 100 105 110 Ala Thr Gln Leu Gly Ser Asp Ser Ser Thr Pro Val Asn Ala Leu Glu 115 120 125 Gln Pro Ser Val Gln Arg Thr Thr Asn Ala Glu Pro Gly Ser Ala Glu 130 135 140 Gln Val Ala Ala Ala Val Leu Pro Ser Val Val Ser Ile Gln Ala Ile 145 150 155 160 Thr Arg Thr Ser Ala Ser Glu Gly Ser Gly Ser Ile Ile Ser Ser Asp 165 170 175 Gly Tyr Val Met Thr Asn Asn His Val Val Ala Gly Ile Glu Gln Ser 180 185 190 Gly Val Leu Glu Val Ser Phe Ser Asp Gly Thr Thr Ala Gln Ala Asp 195 200 205 Phe Ile Ala Gly Asp Pro Ser Thr Asp Ile Ala Val Ile Lys Ile Arg 210 215 220 Asp Val Ser Asn Leu Pro Val Met Ser Phe Gly Asp Ser Asp Ala Leu 225 230 235 240 Gly Val Gly Gln Ser Val Met Ala Val Gly Ser Pro Leu Gly Leu Ser 245 250 255 Ser Thr Val Thr Thr Gly Ile Val Ser Ala Val Asn Arg Pro Val Arg 260 265 270 Ala Ser Gly Asp Gly Gly Glu Ser Ser Leu Ile Asp Ala Ile Gln Thr 275 280 285 Asp Ala Ala Ile Asn Pro Gly Asn Ser Gly Gly Pro Leu Val Asp Met 290 295 300 Asp Gly Asn Leu Ile Gly Met Asn Ser Val Ile Ala Ser Ile Ser Ser 305 310 315 320 Thr Ser Asp Ser Ala Gly Ser Ile Gly Leu Gly Phe Ser Ile Pro Ser 325 330 335 Asn Phe Ala Lys Arg Val Ala Asp Gln Leu Ile Ser Thr Gly Gln Val 340 345 350 Thr Gln Pro Met Ile Gly Val Gln Val Gly Thr Asp Asn Ser Val Thr 355 360 365 Gly Ala Val Ile Ala Ser Val Gln Asp Gly Gly Pro Ala Ala Asp Ala 370 375 380 Gly Leu Gln Pro Gly Asp Ile Val Thr Lys Leu Asn Asp Arg Val Ile 385 390 395 400 Asp Ser Pro Asp Ser Leu Ile Ala Ala Val Arg Ser His Asp Phe Gly 405 410 415 Glu Thr Val Thr Leu Thr Ile Thr Gln Pro Asp Thr Ser Gln Ser Arg 420 425 430 Glu Val Glu Val Thr Leu Thr Ser Glu 435 440 <210> 111 <211> 1174 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1144) <223> RXA02643 <400> 111 tgagaacacc taaaaacctt gcgggaatac caccaacccc atattgttga tatatctaca 60 aactctttta ataagtctga tcaacaacgt gaggaaagca atg aaa aac gtc tcc 115 Met Lys Asn Val Ser 1 5 ttc ggc ctc gac acc ttc ggc gac aac gcc atc gac ctg cag ggc aac 163 Phe Gly Leu Asp Thr Phe Gly Asp Asn Ala Ile Asp Leu Gln Gly Asn 10 15 20 ccg gtc tcc cct gca caa aca ctt cga aac atc att gat gaa gcc aag 211 Pro Val Ser Pro Ala Gln Thr Leu Arg Asn Ile Ile Asp Glu Ala Lys 25 30 35 atg gca gac aaa gtc ggg gtg gat atc atc ggc atc gga gag cac cac 259 Met Ala Asp Lys Val Gly Val Asp Ile Ile Gly Ile Gly Glu His His 40 45 50 cgt gag gaa tac tca gtt tct gca cct gac atc gtc atg aca gct atc 307 Arg Glu Glu Tyr Ser Val Ser Ala Pro Asp Ile Val Met Thr Ala Ile 55 60 65 ctc gca tcc act gag cga ctc aaa gtc acc tct tcc gtg act gtg ctg 355 Leu Ala Ser Thr Glu Arg Leu Lys Val Thr Ser Ser Val Thr Val Leu 70 75 80 85 tcc tct gat gat cct gtt cgc ctg ttt gag cgt tat tcc acc atg aat 403 Ser Ser Asp Asp Pro Val Arg Leu Phe Glu Arg Tyr Ser Thr Met Asn 90 95 100 gca ctg tcc aac ggt cgc gcc gaa atc acc ttg gga cgc ggt tcc ttc 451 Ala Leu Ser Asn Gly Arg Ala Glu Ile Thr Leu Gly Arg Gly Ser Phe 105 110 115 att gag tct ttc cca ttg ttt ggt ttt gat ctt cag gac tac gag cag 499 Ile Glu Ser Phe Pro Leu Phe Gly Phe Asp Leu Gln Asp Tyr Glu Gln 120 125 130 ctg ttt agt gaa cgc ctt gat ttg ttc gcg aag att ctt gag gcc gac 547 Leu Phe Ser Glu Arg Leu Asp Leu Phe Ala Lys Ile Leu Glu Ala Asp 135 140 145 agc cgt ggt cag ggc gtg acc tgg cat ggt gag acc cgc tcg gcg ttg 595 Ser Arg Gly Gln Gly Val Thr Trp His Gly Glu Thr Arg Ser Ala Leu 150 155 160 165 gaa aac cag atg ctt tac cca cca act gag aat ggc att cac gct tgg 643 Glu Asn Gln Met Leu Tyr Pro Pro Thr Glu Asn Gly Ile His Ala Trp 170 175 180 gtt gca gtg ggt ggc agc cca gaa tca gtc gtg cgc gct gct aag tat 691 Val Ala Val Gly Gly Ser Pro Glu Ser Val Val Arg Ala Ala Lys Tyr 185 190 195 cgt ttc ccg ttg atg ctt gcc atc atc ggc ggt gct cct gag cgt ttc 739 Arg Phe Pro Leu Met Leu Ala Ile Ile Gly Gly Ala Pro Glu Arg Phe 200 205 210 cgc ccg tat gtg gat ctg tac aag cgt gcc aac gaa cag ttc ggg cag 787 Arg Pro Tyr Val Asp Leu Tyr Lys Arg Ala Asn Glu Gln Phe Gly Gln 215 220 225 cct caa aag ccc att ggt gtg cac tcc cct gga ctc att gcg gca act 835 Pro Gln Lys Pro Ile Gly Val His Ser Pro Gly Leu Ile Ala Ala Thr 230 235 240 245 gat gag gaa gcc cgt gag cta gca ctt aat gat tgg ttg gaa ctc caa 883 Asp Glu Glu Ala Arg Glu Leu Ala Leu Asn Asp Trp Leu Glu Leu Gln 250 255 260 cgc aag atc ggt gct gaa cgc ggt tgg gct cct gcg gat gca atg cag 931 Arg Lys Ile Gly Ala Glu Arg Gly Trp Ala Pro Ala Asp Ala Met Gln 265 270 275 ttt gaa cgc gaa atc gat cac ggt tcc tta tac atc ggt tcc cct gag 979 Phe Glu Arg Glu Ile Asp His Gly Ser Leu Tyr Ile Gly Ser Pro Glu 280 285 290 acg gtc gca aag aag atc gcc aaa acc att tca gtg ctt gat ctt gat 1027 Thr Val Ala Lys Lys Ile Ala Lys Thr Ile Ser Val Leu Asp Leu Asp 295 300 305 cgc ttt acc ctc aaa tac gcc agt ggc cag acc cct cat gag tac ttg 1075 Arg Phe Thr Leu Lys Tyr Ala Ser Gly Gln Thr Pro His Glu Tyr Leu 310 315 320 325 ctg aag tcc att gag ttg tat ggc act gag gtt att ccg ctg gtg aag 1123 Leu Lys Ser Ile Glu Leu Tyr Gly Thr Glu Val Ile Pro Leu Val Lys 330 335 340 gac atc ttg acc aag cag gct taagaaggtc ttaggacatt cccctgaact 1174 Asp Ile Leu Thr Lys Gln Ala 345 <210> 112 <211> 348 <212> PRT <213> Corynebacterium glutamicum <400> 112 Met Lys Asn Val Ser Phe Gly Leu Asp Thr Phe Gly Asp Asn Ala Ile 1 5 10 15 Asp Leu Gln Gly Asn Pro Val Ser Pro Ala Gln Thr Leu Arg Asn Ile 20 25 30 Ile Asp Glu Ala Lys Met Ala Asp Lys Val Gly Val Asp Ile Ile Gly 35 40 45 Ile Gly Glu His His Arg Glu Glu Tyr Ser Val Ser Ala Pro Asp Ile 50 55 60 Val Met Thr Ala Ile Leu Ala Ser Thr Glu Arg Leu Lys Val Thr Ser 65 70 75 80 Ser Val Thr Val Leu Ser Ser Asp Asp Pro Val Arg Leu Phe Glu Arg 85 90 95 Tyr Ser Thr Met Asn Ala Leu Ser Asn Gly Arg Ala Glu Ile Thr Leu 100 105 110 Gly Arg Gly Ser Phe Ile Glu Ser Phe Pro Leu Phe Gly Phe Asp Leu 115 120 125 Gln Asp Tyr Glu Gln Leu Phe Ser Glu Arg Leu Asp Leu Phe Ala Lys 130 135 140 Ile Leu Glu Ala Asp Ser Arg Gly Gln Gly Val Thr Trp His Gly Glu 145 150 155 160 Thr Arg Ser Ala Leu Glu Asn Gln Met Leu Tyr Pro Pro Thr Glu Asn 165 170 175 Gly Ile His Ala Trp Val Ala Val Gly Gly Ser Pro Glu Ser Val Val 180 185 190 Arg Ala Ala Lys Tyr Arg Phe Pro Leu Met Leu Ala Ile Gly Gly 195 200 205 Ala Pro Glu Arg Phe Arg Pro Tyr Val Asp Leu Tyr Lys Arg Ala Asn 210 215 220 Glu Gln Phe Gly Gln Pro Gln Lys Pro Ile Gly Val His Ser Pro Gly 225 230 235 240 Leu Ile Ala Ala Thr Asp Glu Glu Ala Arg Glu Leu Ala Leu Asn Asp 245 250 255 Trp Leu Glu Leu Gln Arg Lys Ile Gly Ala Glu Arg Gly Trp Ala Pro 260 265 270 Ala Asp Ala Met Gln Phe Glu Arg Glu Ile Asp His Gly Ser Leu Tyr 275 280 285 Ile Gly Ser Pro Glu Thr Val Ala Lys Lys Ile Ala Lys Thr Ile Ser 290 295 300 Val Leu Asp Leu Asp Arg Phe Thr Leu Lys Tyr Ala Ser Gly Gln Thr 305 310 315 320 Pro His Glu Tyr Leu Leu Lys Ser Ile Glu Leu Tyr Gly Thr Glu Val 325 330 335 Ile Pro Leu Val Lys Asp Ile Leu Thr Lys Gln Ala 340 345 <210> 113 <211> 1546 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1516) <223> RXA02705 <400> 113 cgtgaccatc cgtttctggc tgatcgcgat catggctgtg ttggcgggtg tcggtgtgtt 60 ttacagcgac tggctccact tagcggaggt ataaataatt atg gtt tct ctg tcc 115 Met Val Ser Leu Ser 1 5 cat tta cct cag gcg ctg cag ggc cgt att ctt gtg gcc ggc gct ggt 163 His Leu Pro Gln Ala Leu Gln Gly Arg Ile Leu Val Ala Gly Ala Gly 10 15 20 gtt tcc ggc ctg tcc att gca aag atg ctc agt gag ttg cat tgc gat 211 Val Ser Gly Leu Ser Ile Ala Lys Met Leu Ser Glu Leu His Cys Asp 25 30 35 gtt gtg gtc gcc gac gat aac gaa act gca cgt cac atg ctc att gaa 259 Val Val Val Ala Asp Asp Asn Glu Thr Ala Arg His Met Leu Ile Glu 40 45 50 gta gta gac gtt gca gat atc agc acc gcg cag gct cag gaa cag ctg 307 Val Val Asp Val Ala Asp Ile Ser Thr Ala Gln Ala Gln Glu Gln Leu 55 60 65 gat tct ttc tcc att gtg gtc acc tcc ccg ggc tgg cgc cca aca agc 355 Asp Ser Phe Ser Ile Val Val Thr Ser Pro Gly Trp Arg Pro Thr Ser 70 75 80 85 act ttg ctt gtc gac gcc cac cgc cag ggc ctt gag gtt atc ggt gac 403 Thr Leu Leu Val Asp Ala His Arg Gln Gly Leu Glu Val Ile Gly Asp 90 95 100 gtc gag ctt gct tgg cgc ctg gac cag gca ggt gtt ttc ggc gag cca 451 Val Glu Leu Ala Trp Arg Leu Asp Gln Ala Gly Val Phe Gly Glu Pro 105 110 115 cat acg tgg ctc gca gtc acc ggc acc aac ggt aaa acc acc aca 499 His Thr Trp Leu Ala Val Thr Gly Thr Asn Gly Lys Thr Thr Thr Thr 120 125 130 tca atg ctc gcc gcg atg atg aat gag ggc ggt ttt act gcc aag gca 547 Ser Met Leu Ala Ala Met Met Asn Glu Gly Gly Phe Thr Ala Lys Ala 135 140 145 gtg ggc aat atc ggc atc ccg gtg tct gag gct ttg gta gcg aaa aac 595 Val Gly Asn Ile Gly Ile Pro Val Ser Glu Ala Leu Val Ala Lys Asn 150 155 160 165 cgc att gat gtg tta gtt gca gag ctg tct agt ttc caa ttg cac tgg 643 Arg Ile Asp Val Leu Val Ala Glu Leu Ser Ser Phe Gln Leu His Trp 170 175 180 tct cca acc ttc acc cct gat gct ggc gtg gtg ctc aac ttg gct gag 691 Ser Pro Thr Phe Thr Pro Asp Ala Gly Val Val Leu Asn Leu Ala Glu 185 190 195 gat cac atc gat tgg cac ggt tcc atg cgt gat tat gcg ttg gcc aag 739 Asp His Ile Asp Trp His Gly Ser Met Arg Asp Tyr Ala Leu Ala Lys 200 205 210 atg gaa gtg ctc aag ggc aag gtc gcc atc att ggg gca gac gat cct 787 Met Glu Val Leu Lys Gly Lys Val Ala Ile Ile Gly Ala Asp Asp Pro 215 220 225 tat ttg gtg cag ctg act tct gaa gca gac ttg agt ggt ctc att gga 835 Tyr Leu Val Gln Leu Thr Ser Glu Ala Asp Leu Ser Gly Leu Ile Gly 230 235 240 245 ttt acc gtc aat gag cct gca acc ggc cag ttg ggt gtg aaa gcg ggg 883 Phe Thr Val Asn Glu Pro Ala Thr Gly Gln Leu Gly Val Lys Ala Gly 250 255 260 gag ctc gtc gat aat gcc tac ggc aat aat gtg gtg ctt gca tcc gct 931 Glu Leu Val Asp Asn Ala Tyr Gly Asn Asn Val Val Leu Ala Ser Ala 265 270 275 gac ggc att aat ccc gcc ggc cct gcc ggt gtt ttg gac gct ttg gct 979 Asp Gly Ile Asn Pro Ala Gly Pro Ala Gly Val Leu Asp Ala Leu Ala 280 285 290 gca gct gcg gtg gcg cgc tcg cag ggc gtg gca cct gag gcg atc gcg 1027 Ala Ala Ala Val Ala Arg Ser Gln Gly Val Ala Pro Glu Ala Ile Ala 295 300 305 cgt gcg ttg gat tct ttt gag gtg gca ggc cac cgt ggc cag gtc gtc 1075 Arg Ala Leu Asp Ser Phe Glu Val Ala Gly His Arg Gly Gln Val Val 310 315 320 325 gcc gag cat gac ggt gtt cat ttc att gac aac tcc aag gcg acc aac 1123 Ala Glu His Asp Gly Val His Phe Ile Asp Asn Ser Lys Ala Thr Asn 330 335 340 ccc cac gct gct gat tct gcg cta gct ggg cat gat tca gtc att tgg 1171 Pro His Ala Ala Asp Ser Ala Leu Ala Gly His Asp Ser Val Ile Trp 345 350 355 gtt gtc ggc gga cag ctc aaa ggc gcg gac att gcg cca ctg gtg aaa 1219 Val Val Gly Gly Gln Leu Lys Gly Ala Asp Ile Ala Pro Leu Val Lys 360 365 370 aag cac gaa cag cgc atc aag gca gca ttg gtg ttg ggc gca gat cgt 1267 Lys His Glu Gln Arg Ile Lys Ala Ala Leu Val Leu Gly Ala Asp Arg 375 380 385 gct gaa atc gtg gca gcg ttg aag gaa cac gcg tcg cag gcc tct gta 1315 Ala Glu Ile Val Ala Ala Leu Lys Glu His Ala Ser Gln Ala Ser Val 390 395 400 405 ttt gtc act gac aag acg gag cca ttt gag gca atg gaa gaa atc gtc 1363 Phe Val Thr Asp Lys Thr Glu Pro Phe Glu Ala Met Glu Glu Ile Val 410 415 420 act gag gca ttt agc atc agc gaa ccc ggc gat acc gtg ttg ctt gcc 1411 Thr Glu Ala Phe Ser Ile Ser Glu Pro Gly Asp Thr Val Leu Leu Ala 425 430 435 cct gcc gct gcg tct ttg gac atg ttc aaa ggc atg ggc cag cgt ggc 1459 Pro Ala Ala Ala Ser Leu Asp Met Phe Lys Gly Met Gly Gln Arg Gly 440 445 450 gac ctc ttt gca cac aac atc att ggc aca atc aaa gga tta acg gaa 1507 Asp Leu Phe Ala His Asn Ile Ile Gly Thr Ile Lys Gly Leu Thr Glu 455 460 465 gag aaa ggc tgatcatgac caccggagcc tcaaaaaaac 1546 Glu Lys Gly 470 <210> 114 <211> 472 <212> PRT <213> Corynebacterium glutamicum <400> 114 Met Val Ser Leu Ser His Leu Pro Gln Ala Leu Gln Gly Arg Ile Leu 1 5 10 15 Val Ala Gly Ala Gly Val Ser Gly Leu Ser Ile Ala Lys Met Leu Ser 20 25 30 Glu Leu His Cys Asp Val Val Val Ala Asp Asp Asn Glu Thr Ala Arg 35 40 45 His Met Leu Ile Glu Val Val Asp Val Ala Asp Ile Ser Thr Ala Gln 50 55 60 Ala Gln Glu Gln Leu Asp Ser Phe Ser Ile Val Val Thr Ser Pro Gly 65 70 75 80 Trp Arg Pro Thr Ser Thr Leu Leu Val Asp Ala His Arg Gln Gly Leu 85 90 95 Glu Val Ile Gly Asp Val Glu Leu Ala Trp Arg Leu Asp Gln Ala Gly 100 105 110 Val Phe Gly Glu Pro His Thr Trp Leu Ala Val Thr Gly Thr Asn Gly 115 120 125 Lys Thr Thr Thr Thr Thr Ser Met Leu Ala Ala Met Met Asn Glu Gly Gly 130 135 140 Phe Thr Ala Lys Ala Val Gly Asn Ile Gly Ile Pro Val Ser Glu Ala 145 150 155 160 Leu Val Ala Lys Asn Arg Ile Asp Val Leu Val Ala Glu Leu Ser Ser 165 170 175 Phe Gln Leu His Trp Ser Pro Thr Phe Thr Pro Asp Ala Gly Val Val 180 185 190 Leu Asn Leu Ala Glu Asp His Ile Asp Trp His Gly Ser Met Arg Asp 195 200 205 Tyr Ala Leu Ala Lys Met Glu Val Leu Lys Gly Lys Val Ala Ile Ile 210 215 220 Gly Ala Asp Asp Pro Tyr Leu Val Gln Leu Thr Ser Glu Ala Asp Leu 225 230 235 240 Ser Gly Leu Ile Gly Phe Thr Val Asn Glu Pro Ala Thr Gly Gln Leu 245 250 255 Gly Val Lys Ala Gly Glu Leu Val Asp Asn Ala Tyr Gly Asn Asn Val 260 265 270 Val Leu Ala Ser Ala Asp Gly Ile Asn Pro Ala Gly Pro Ala Gly Val 275 280 285 Leu Asp Ala Leu Ala Ala Ala Ala Val Ala Arg Ser Gln Gly Val Ala 290 295 300 Pro Glu Ala Ile Ala Arg Ala Leu Asp Ser Phe Glu Val Ala Gly His 305 310 315 320 Arg Gly Gln Val Val Ala Glu His Asp Gly Val His Phe Ile Asp Asn 325 330 335 Ser Lys Ala Thr Asn Pro His Ala Ala Asp Ser Ala Leu Ala Gly His 340 345 350 Asp Ser Val Ile Trp Val Val Gly Gly Gln Leu Lys Gly Ala Asp Ile 355 360 365 Ala Pro Leu Val Lys Lys His Glu Gln Arg Ile Lys Ala Ala Leu Val 370 375 380 Leu Gly Ala Asp Arg Ala Glu Ile Val Ala Ala Leu Lys Glu His Ala 385 390 395 400 Ser Gln Ala Ser Val Phe Val Thr Asp Lys Thr Glu Pro Phe Glu Ala 405 410 415 Met Glu Glu Ile Val Thr Glu Ala Phe Ser Ile Ser Glu Pro Gly Asp 420 425 430 Thr Val Leu Leu Ala Pro Ala Ala Ala Ser Leu Asp Met Phe Lys Gly 435 440 445 Met Gly Gln Arg Gly Asp Leu Phe Ala His Asn Ile Gly Thr Ile 450 455 460 Lys Gly Leu Thr Glu Glu Lys Gly 465 470 <210> 115 <211> 1693 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) .. (1663) <223> RXA02710 <400> 115 gaatttcata atctgaactt ttgtttgaac tcttttcggc atcacccacg tgccgcgtcc 60 gaattattaa cacctagaaa cctgtggagg agagaaaacc atg gca acc acg ttg 115 Met Ala Thr Thr Leu 1 5 ctg gac ctc acc aaa ctt atc gat ggc atc ctc aag ggc tct gcc cag 163 Leu Asp Leu Thr Lys Leu Ile Asp Gly Ile Leu Lys Gly Ser Ala Gln 10 15 20 ggc gtt ccc gct cac gca gta ggg gaa caa gca atc gcg gct att ggt 211 Gly Val Pro Ala His Ala Val Gly Glu Gln Ala Ile Ala Ala Ile Gly 25 30 35 ctt gac tcc tcc agc tta cct acc tcg gac gct att ttt gct gca gtt 259 Leu Asp Ser Ser Ser Leu Pro Thr Ser Asp Ala Ile Phe Ala Ala Val 40 45 50 cca gga acc cgc act cac ggc gca cag ttt gca ggt acg gat aac gct 307 Pro Gly Thr Arg Thr His Gly Ala Gln Phe Ala Gly Thr Asp Asn Ala 55 60 65 gcg aaa gct gtg gcc att ttg act gac gca gct gga ctt gag gtg ctc 355 Ala Lys Ala Val Ala Ile Leu Thr Asp Ala Ala Gly Leu Glu Val Leu 70 75 80 85 aac gaa gca gga gag acc cgc cca gtc atc gtt gtt gat gat gtc cgc 403 Asn Glu Ala Gly Glu Thr Arg Pro Val Ile Val Val Asp Asp Val Arg 90 95 100 gca gta ctt ggc gca gca tca tca agc att tat ggc gat cct tca aaa 451 Ala Val Leu Gly Ala Ala Ser Ser Ser Ile Tyr Gly Asp Pro Ser Lys 105 110 115 gat ttc acg ctc att gga gtc act gga acc tca ggt aaa acc acc acc 499 Asp Phe Thr Leu Ile Gly Val Thr Gly Thr Ser Gly Lys Thr Thr Thr 120 125 130 agc tac ctc ttg gaa aaa gga ctc atg gag gca ggc cac aaa gtt ggt 547 Ser Tyr Leu Leu Glu Lys Gly Leu Met Glu Ala Gly His Lys Val Gly 135 140 145 ttg atc ggc acc aca ggt aca cgt att gac ggg gaa gaa gta ccc aca 595 Leu Ile Gly Thr Thr Gly Thr Arg Ile Asp Gly Glu Glu Val Pro Thr 150 155 160 165 aag ctc acc act cca gaa gcg ccg act ctg cag gca ttg ttt gct cga 643 Lys Leu Thr Thr Pro Glu Ala Pro Thr Leu Gln Ala Leu Phe Ala Arg 170 175 180 atg cgc gat cac ggt gtc acc cac gtg gtg atg gaa gta tcc agc cat 691 Met Arg Asp His Gly Val Thr His Val Val Met Glu Val Ser Ser His 185 190 195 gca ttg tca ttg ggc aga gtt gcg ggt tcc cac ttt gat gta gct gcg 739 Ala Leu Ser Leu Gly Arg Val Ala Gly Ser His Phe Asp Val Ala Ala 200 205 210 ttt acc aac ctg tcg cag gat cac ctt gat ttc cac ccc acc atg gat 787 Phe Thr Asn Leu Ser Gln Asp His Leu Asp Phe His Pro Thr Met Asp 215 220 225 gat tac ttt gac gcg aag gca ttg ttc ttc cgc gca gat tct cca ctt 835 Asp Tyr Phe Asp Ala Lys Ala Leu Phe Phe Arg Ala Asp Ser Pro Leu 230 235 240 245 gtg gct gac aaa cag gtc gtg tgc gtg gat gat tct tgg ggt cag cgc 883 Val Ala Asp Lys Gln Val Val Cys Val Asp Asp Ser Trp Gly Gln Arg 250 255 260 atg gcc agc gtg gca gcg gat gtg caa aca gta tcc acc ctt ggg caa 931 Met Ala Ser Val Ala Ala Asp Val Gln Thr Val Ser Thr Leu Gly Gln 265 270 275 gaa gca gac ttc agc gct aca gac atc aat gtc agc gac tct ggc gcc 979 Glu Ala Asp Phe Ser Ala Thr Asp Ile Asn Val Ser Asp Ser Gly Ala 280 285 290 cag agt ttt aag atc aac gcc ccc tca aac cag tcc tac cag gtc gag 1027 Gln Ser Phe Lys Ile Asn Ala Pro Ser Asn Gln Ser Tyr Gln Val Glu 295 300 305 cta gct ctt cca ggt gcg ttc aac gtt gct aac gcc acg ttg gca ttt 1075 Leu Ala Leu Pro Gly Ala Phe Asn Val Ala Asn Ala Thr Leu Ala Phe 310 315 320 325 gcc gct gcg gca cgc gtg ggt gtt gat ggc gaa gcg ttt gct cga ggc 1123 Ala Ala Ala Ala Arg Val Gly Val Asp Gly Glu Ala Phe Ala Arg Gly 330 335 340 atg tcc aag gtc gcg gtt cca ggc cgt atg gaa cgc att gat gag gga 1171 Met Ser Lys Val Ala Val Pro Gly Arg Met Glu Arg Ile Asp Glu Gly 345 350 355 caa gac ttc ctt gca gtg gtg gat tat gcc cac aag cct gct gca gtg 1219 Gln Asp Phe Leu Ala Val Val Asp Tyr Ala His Lys Pro Ala Ala Val 360 365 370 gct gct gtg ttg gat acg ttg agg acc cag att gac ggg cgc ctc gga 1267 Ala Ala Val Leu Asp Thr Leu Arg Thr Gln Ile Asp Gly Arg Leu Gly 375 380 385 gtg gtt atc ggt gct ggt gga gac cgc gat tcc acc aag cgt ggc ccc 1315 Val Val Ile Gly Ala Gly Gly Asp Arg Asp Ser Thr Lys Arg Gly Pro 390 395 400 405 atg ggg cag ttg tcc gca cag cgt gct gat cta gtt att gtc act gat 1363 Met Gly Gln Leu Ser Ala Gln Arg Ala Asp Leu Val Ile Val Thr Asp 410 415 420 gac aac cct cgt tca gag gtg cct gcc acg att cgc gca gca gtc act 1411 Asp Asn Pro Arg Ser Glu Val Pro Ala Thr Ile Arg Ala Ala Val Thr 425 430 435 gca gga gca cag cag ggt gct tca gag tcc gaa cga ccg gtg gaa gtc 1459 Ala Gly Ala Gln Gln Gly Ala Ser Glu Ser Glu Arg Pro Val Glu Val 440 445 450 cta gaa att ggt gac cgt gca gaa gca att cgc gtt ttg gtc gag tgg 1507 Leu Glu Ile Gly Asp Arg Ala Glu Ala Ile Arg Val Leu Val Glu Trp 455 460 465 gca cag cct gga gat ggc att gta gta gct gga aaa ggc cat gaa gtt 1555 Ala Gln Pro Gly Asp Gly Ile Val Val Ala Gly Lys Gly His Glu Val 470 475 480 485 gga caa cta gtt gct ggt gtc acc cac cat ttt gat gac cgc gaa gaa 1603 Gly Gln Leu Val Ala Gly Val Thr His His Phe Asp Asp Arg Glu Glu 490 495 500 gtt cgc gct gct ttg aca gaa aag ctc aac aat aaa ctt ccc ctt act 1651 Val Arg Ala Ala Leu Thr Glu Lys Leu Asn Asn Lys Leu Pro Leu Thr 505 510 515 acg gaa gaa gga taggccacag tcatgatcac aatgaccctt 1693 Thr Glu Glu Gly 520 <210> 116 <211> 521 <212> PRT <213> Corynebacterium glutamicum <400> 116 Met Ala Thr Thr Leu Leu Asp Leu Thr Lys Leu Ile Asp Gly Ile Leu 1 5 10 15 Lys Gly Ser Ala Gln Gly Val Pro Ala His Ala Val Gly Glu Gln Ala 20 25 30 Ile Ala Ala Ile Gly Leu Asp Ser Ser Ser Leu Pro Thr Ser Asp Ala 35 40 45 Ile Phe Ala Ala Val Pro Gly Thr Arg Thr His Gly Ala Gln Phe Ala 50 55 60 Gly Thr Asp Asn Ala Ala Lys Ala Val Ala Ile Leu Thr Asp Ala Ala 65 70 75 80 Gly Leu Glu Val Leu Asn Glu Ala Gly Glu Thr Arg Pro Val Ile Val 85 90 95 Val Asp Asp Val Arg Ala Val Leu Gly Ala Ala Ser Ser Ser Ile Tyr 100 105 110 Gly Asp Pro Ser Lys Asp Phe Thr Leu Ile Gly Val Thr Gly Thr Ser 115 120 125 Gly Lys Thr Thr Thr Ser Ser Tyr Leu Leu Glu Lys Gly Leu Met Glu Ala 130 135 140 Gly His Lys Val Gly Leu Ile Gly Thr Thr Gly Thr Arg Ile Asp Gly 145 150 155 160 Glu Glu Val Pro Thr Lys Leu Thr Thr Pro Glu Ala Pro Thr Leu Gln 165 170 175 Ala Leu Phe Ala Arg Met Arg Asp His Gly Val Thr His Val Val Met 180 185 190 Glu Val Ser Ser His Ala Leu Ser Leu Gly Arg Val Ala Gly Ser His 195 200 205 Phe Asp Val Ala Ala Phe Thr Asn Leu Ser Gln Asp His Leu Asp Phe 210 215 220 His Pro Thr Met Asp Asp Tyr Phe Asp Ala Lys Ala Leu Phe Phe Arg 225 230 235 240 Ala Asp Ser Pro Leu Val Ala Asp Lys Gln Val Val Cys Val Asp Asp 245 250 255 Ser Trp Gly Gln Arg Met Ala Ser Val Ala Ala Asp Val Gln Thr Val 260 265 270 Ser Thr Leu Gly Gln Glu Ala Asp Phe Ser Ala Thr Asp Ile Asn Val 275 280 285 Ser Asp Ser Gly Ala Gln Ser Phe Lys Ile Asn Ala Pro Ser Asn Gln 290 295 300 Ser Tyr Gln Val Glu Leu Ala Leu Pro Gly Ala Phe Asn Val Ala Asn 305 310 315 320 Ala Thr Leu Ala Phe Ala Ala Ala Ala Arg Val Gly Val Asp Gly Glu 325 330 335 Ala Phe Ala Arg Gly Met Ser Lys Val Ala Val Pro Gly Arg Met Glu 340 345 350 Arg Ile Asp Glu Gly Gln Asp Phe Leu Ala Val Val Asp Tyr Ala His 355 360 365 Lys Pro Ala Ala Val Ala Ala Val Leu Asp Thr Leu Arg Thr Gln Ile 370 375 380 Asp Gly Arg Leu Gly Val Val Ile Gly Ala Gly Gly Asp Arg Asp Ser 385 390 395 400 Thr Lys Arg Gly Pro Met Gly Gln Leu Ser Ala Gln Arg Ala Asp Leu 405 410 415 Val Ile Val Thr Asp Asp Asn Pro Arg Ser Glu Val Pro Ala Thr Ile 420 425 430 Arg Ala Ala Val Thr Ala Gly Ala Gln Gln Gly Ala Ser Glu Ser Glu 435 440 445 Arg Pro Val Glu Val Leu Glu Ile Gly Asp Arg Ala Glu Ala Ile Arg 450 455 460 Val Leu Val Glu Trp Ala Gln Pro Gly Asp Gly Ile Val Val Ala Gly 465 470 475 480 Lys Gly His Glu Val Gly Gln Leu Val Ala Gly Val Thr His His Phe 485 490 495 Asp Asp Arg Glu Glu Val Arg Ala Ala Leu Thr Glu Lys Leu Asn Asn 500 505 510 Lys Leu Pro Leu Thr Thr Glu Glu Gly 515 520 <210> 117 <211> 2242 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (101) .. (2212) <223> RXA02711 <400> 117 aaaacctcca ggcgattcca caagaagcag cagctccgcc gtatcagacc aacactgttc 60 cttatgctgc aaccaccgga caagcaggtg gcgcagggca gtg act ttc ccc agc 115 Val Thr Phe Pro Ser 1 5 aat ggc aga agt cgg ggc gag cgt gcg gga cgt gaa gat acg tcc cgc 163 Asn Gly Arg Ser Arg Gly Glu Arg Ala Gly Arg Glu Asp Thr Ser Arg 10 15 20 cgt tcg gcg tat cag gac gaa agc aga aga gcc gct aga gag cgc gaa 211 Arg Ser Ala Tyr Gln Asp Glu Ser Arg Arg Ala Ala Arg Glu Arg Glu 25 30 35 ctt acg cga cgc agc ggt aaa gct aaa ggc gta aac caa gaa gaa gga 259 Leu Thr Arg Arg Ser Gly Lys Ala Lys Gly Val Asn Gln Glu Glu Gly 40 45 50 gtg acc tac cgg cct aaa tct tca acc cag ggc ggc gca cgc aag cga 307 Val Thr Tyr Arg Pro Lys Ser Ser Thr Gln Gly Gly Ala Arg Lys Arg 55 60 65 cgt gtg aac atg gtt acc gct atc gca ttg gtc atc gct ggc gta ctg 355 Arg Val Asn Met Val Thr Ala Ile Ala Leu Val Ile Ala Gly Val Leu 70 75 80 85 atc att cgc ctc ggc tgg gtc caa gtt gtc tgg gga cca gaa ctg tcc 403 Ile Ile Arg Leu Gly Trp Val Gln Val Val Trp Gly Pro Glu Leu Ser 90 95 100 ctc aat gct tcg gaa cag cgc acc cgc gtg tac gta gat cct gca cgt 451 Leu Asn Ala Ser Glu Gln Arg Thr Arg Val Tyr Val Asp Pro Ala Arg 105 110 115 cgt gga agc atc gtg gac cgc gaa gga aac cag atg gcg tac acg atg 499 Arg Gly Ser Ile Val Asp Arg Glu Gly Asn Gln Met Ala Tyr Thr Met 120 125 130 cag gca cgt tcg ctg acg gtt tct ccg aac atc atg cgt gag gaa tta 547 Gln Ala Arg Ser Leu Thr Val Ser Pro Asn Ile Met Arg Glu Glu Leu 135 140 145 aag acc gga act gat ttg gcc ttg cgt ttg gcg gct gaa gaa acc gat 595 Lys Thr Gly Thr Asp Leu Ala Leu Arg Leu Ala Ala Glu Glu Thr Asp 150 155 160 165 ccg gaa aac gtg gcc agc tat gtg acc atc gaa gaa ggc aac gcg tat 643 Pro Glu Asn Val Ala Ser Tyr Val Thr Ile Glu Glu Gly Asn Ala Tyr 170 175 180 gtt ttt gcg tct gaa gaa cag cgc gaa acc att ctg tcc gac aag gta 691 Val Phe Ala Ser Glu Glu Gln Arg Glu Thr Ile Leu Ser Asp Lys Val 185 190 195 gaa gag cgc att caa agc att gcg gat cgg atc cct gag atc atc aaa 739 Glu Glu Arg Ile Gln Ser Ile Ala Asp Arg Ile Pro Glu Ile Ile Lys 200 205 210 tcc cat gac caa gat gtc act gga att tcc tct gag gag atc ctg gac 787 Ser His Asp Gln Asp Val Thr Gly Ile Ser Ser Glu Glu Ile Leu Asp 215 220 225 aag ctc aat gct gat agc cag tat gag gtg ctc gtc cgc aat gtt gat 835 Lys Leu Asn Ala Asp Ser Gln Tyr Glu Val Leu Val Arg Asn Val Asp 230 235 240 245 ccc gat gta gcg tca gaa atc acc gat gag atg ccc agc gtc gca gct 883 Pro Asp Val Ala Ser Glu Ile Thr Asp Glu Met Pro Ser Val Ala Ala 250 255 260 gat cat caa gac atc cgc caa tac cca aac ggc gcg att ggt gaa aac 931 Asp His Gln Asp Ile Arg Gln Tyr Pro Asn Gly Ala Ile Gly Glu Asn 265 270 275 atc atc ggt cga atc agc atg gac ggc gaa ggc cag ttc ggc ttt gag 979 Ile Ile Gly Arg Ile Ser Met Asp Gly Glu Gly Gln Phe Gly Phe Glu 280 285 290 gct tcc aac gat tcc ctg ttg gca gga aac aac ggt cgc tca acc cag 1027 Ala Ser Asn Asp Ser Leu Leu Ala Gly Asn Asn Gly Arg Ser Thr Gln 295 300 305 gac atg tcc att ttg gga caa gca ata ccg ggc acg ttg agg gat caa 1075 Asp Met Ser Ile Leu Gly Gln Ala Ile Pro Gly Thr Leu Arg Asp Gln 310 315 320 325 att cca gcc att gat ggt gcc agc gtt gaa ctc acc gtt gat ctg gat 1123 Ile Pro Ala Ile Asp Gly Ala Ser Val Glu Leu Thr Val Asp Leu Asp 330 335 340 ctg caa acc tat gtg cag cag gca ttg gag cag gcg aaa gct aac tcc 1171 Leu Gln Thr Tyr Val Gln Gln Ala Leu Glu Gln Ala Lys Ala Asn Ser 345 350 355 ggt gca gaa aac gcc tcc gct gtg gtt ctt gat gcc aag acc gct gag 1219 Gly Ala Glu Asn Ala Ser Ala Val Val Leu Asp Ala Lys Thr Ala Glu 360 365 370 gtt ttg gcg atg gca aac acc gat acc atc aac ccc aac gaa gac acg 1267 Val Leu Ala Met Ala Asn Thr Asp Thr Ile Asn Pro Asn Glu Asp Thr 375 380 385 gga aag cag att gag cag ggc aag agc ttt gac aat cct tct gtc acc 1315 Gly Lys Gln Ile Glu Gln Gly Lys Ser Phe Asp Asn Pro Ser Val Thr 390 395 400 405 cac ccc ttc gag cct ggt tct gta gcc aag gtg att act gca gca ggc 1363 His Pro Phe Glu Pro Gly Ser Val Ala Lys Val Ile Thr Ala Ala Gly 410 415 420 gta att caa gag ggc ttg act act cca gat gaa gtg ttg cag gta ccg 1411 Val Ile Gln Glu Gly Leu Thr Thr Pro Asp Glu Val Leu Gln Val Pro 425 430 435 ggc agt att gaa atg gcc ggt gtt tct gtc ggt gat gcg tgg gac cac 1459 Gly Ser Ile Glu Met Ala Gly Val Ser Val Gly Asp Ala Trp Asp His 440 445 450 ggt gtc gtt ccc tac acc act gca gga att ttt ggt aag tcc tcg aat 1507 Gly Val Val Pro Tyr Thr Thr Ala Gly Ile Phe Gly Lys Ser Ser Asn 455 460 465 gta ggc act ctg atg ctt gcg cag cgt ctt ggt gaa gat aaa ttt gct 1555 Val Gly Thr Leu Met Leu Ala Gln Arg Leu Gly Glu Asp Lys Phe Ala 470 475 480 485 gat tac ctg gaa cga ttc ggt gtg gga cag cca acg ggt att gag ctt 1603 Asp Tyr Leu Glu Arg Phe Gly Val Gly Gln Pro Thr Gly Ile Glu Leu 490 495 500 ccg agc gaa tcc caa ggc ctg ctg ccc gca cgt gag cag tgg tct ggc 1651 Pro Ser Glu Ser Gln Gly Leu Leu Pro Ala Arg Glu Gln Trp Ser Gly 505 510 515 ggt act ttt gct aac ctg ccc atc ggt cag ggt atg tcg atc acc acg 1699 Gly Thr Phe Ala Asn Leu Pro Ile Gly Gln Gly Met Ser Ile Thr Thr 520 525 530 ttg caa atg gct gga atc tac caa gcc ttg gcc aac gat ggt gaa cgc 1747 Leu Gln Met Ala Gly Ile Tyr Gln Ala Leu Ala Asn Asp Gly Glu Arg 535 540 545 att caa ccg cgg atc atc aag agc gtg act gat tct gac gga aca gtc 1795 Ile Gln Pro Arg Ile Ile Lys Ser Val Thr Asp Ser Asp Gly Thr Val 550 555 560 565 cta gag cag cca gaa ccc gat aaa atc cag gtt gtc agc gct gaa gct 1843 Leu Glu Gln Pro Glu Pro Asp Lys Ile Gln Val Val Ser Ala Glu Ala 570 575 580 gcc cgc acc acg gtg gat atg ttt agg tct gtc acc cag gtt gat cca 1891 Ala Arg Thr Thr Val Asp Met Phe Arg Ser Val Thr Gln Val Asp Pro 585 590 595 act gga gtg caa caa ggt acc gct cca gac gcc tcc att gag ggt tat 1939 Thr Gly Val Gln Gln Gly Thr Ala Pro Asp Ala Ser Ile Glu Gly Tyr 600 605 610 caa atc tca ggt aag aca ggt acg gcg cag aaa gtt gac ccc aac acg 1987 Gln Ile Ser Gly Lys Thr Gly Thr Ala Gln Lys Val Asp Pro Asn Thr 615 620 625 ggc gcg tac tct aac tcg caa tac tgg att acc ttc gcg ggt att gca 2035 Gly Ala Tyr Ser Asn Ser Gln Tyr Trp Ile Thr Phe Ala Gly Ile Ala 630 635 640 645 ccc gct gat gat cct cga ttt gtt gta gcc atc atg ctt gat gag cca 2083 Pro Ala Asp Asp Pro Arg Phe Val Val Ala Ile Met Leu Asp Glu Pro 650 655 660 gaa cgc gga gtc cac ggt ggt ggc ggc caa acc gca gca cct ttg ttc 2131 Glu Arg Gly Val His Gly Gly Gly Gly Gln Thr Ala Ala Pro Leu Phe 665 670 675 aaa gac atc gcc acc tgg ttg ctc aac cgc gac aac atc cca ctg tct 2179 Lys Asp Ile Ala Thr Trp Leu Leu Asn Arg Asp Asn Ile Pro Leu Ser 680 685 690 gca gcc acc gaa ccg atc atc ctt caa gct caa taactcaaac agaagtgtct 2232 Ala Ala Thr Glu Pro Ile Ile Leu Gln Ala Gln 695 700 tttgtagaat 2242 <210> 118 <211> 704 <212> PRT <213> Corynebacterium glutamicum <400> 118 Val Thr Phe Pro Ser Asn Gly Arg Ser Arg Gly Glu Arg Ala Gly Arg 1 5 10 15 Glu Asp Thr Ser Arg Arg Ser Ala Tyr Gln Asp Glu Ser Arg Arg Ala 20 25 30 Ala Arg Glu Arg Glu Leu Thr Arg Arg Ser Gly Lys Ala Lys Gly Val 35 40 45 Asn Gln Glu Glu Gly Val Thr Tyr Arg Pro Lys Ser Ser Thr Gln Gly 50 55 60 Gly Ala Arg Lys Arg Arg Val Asn Met Val Thr Ala Ile Ala Leu Val 65 70 75 80 Ile Ala Gly Val Leu Ile Ile Arg Leu Gly Trp Val Gln Val Val Trp 85 90 95 Gly Pro Glu Leu Ser Leu Asn Ala Ser Glu Gln Arg Thr Arg Val Tyr 100 105 110 Val Asp Pro Ala Arg Arg Gly Ser Ile Val Asp Arg Glu Gly Asn Gln 115 120 125 Met Ala Tyr Thr Met Gln Ala Arg Ser Leu Thr Val Ser Pro Asn Ile 130 135 140 Met Arg Glu Glu Leu Lys Thr Gly Thr Asp Leu Ala Leu Arg Leu Ala 145 150 155 160 Ala Glu Glu Thr Asp Pro Glu Asn Val Ala Ser Tyr Val Thr Ile Glu 165 170 175 Glu Gly Asn Ala Tyr Val Phe Ala Ser Glu Glu Gln Arg Glu Thr Ile 180 185 190 Leu Ser Asp Lys Val Glu Glu Arg Ile Gln Ser Ile Ala Asp Arg Ile 195 200 205 Pro Glu Ile Ile Lys Ser His Asp Gln Asp Val Thr Gly Ile Ser Ser 210 215 220 Glu Glu Ile Leu Asp Lys Leu Asn Ala Asp Ser Gln Tyr Glu Val Leu 225 230 235 240 Val Arg Asn Val Asp Pro Asp Val Ala Ser Glu Ile Thr Asp Glu Met 245 250 255 Pro Ser Val Ala Ala Asp His Gln Asp Ile Arg Gln Tyr Pro Asn Gly 260 265 270 Ala Ile Gly Glu Asn Ile Gly Arg Ile Ser Met Asp Gly Glu Gly 275 280 285 Gln Phe Gly Phe Glu Ala Ser Asn Asp Ser Leu Leu Ala Gly Asn Asn 290 295 300 Gly Arg Ser Thr Gln Asp Met Ser Ile Leu Gly Gln Ala Ile Pro Gly 305 310 315 320 Thr Leu Arg Asp Gln Ile Pro Ala Ile Asp Gly Ala Ser Val Glu Leu 325 330 335 Thr Val Asp Leu Asp Leu Gln Thr Tyr Val Gln Gln Ala Leu Glu Gln 340 345 350 Ala Lys Ala Asn Ser Gly Ala Glu Asn Ala Ser Ala Val Val Leu Asp 355 360 365 Ala Lys Thr Ala Glu Val Leu Ala Met Ala Asn Thr Asp Thr Ile Asn 370 375 380 Pro Asn Glu Asp Thr Gly Lys Gln Ile Glu Gln Gly Lys Ser Phe Asp 385 390 395 400 Asn Pro Ser Val Thr His Pro Phe Glu Pro Gly Ser Val Ala Lys Val 405 410 415 Ile Thr Ala Ala Gly Val Ile Gln Glu Gly Leu Thr Thr Pro Asp Glu 420 425 430 Val Leu Gln Val Pro Gly Ser Ile Glu Met Ala Gly Val Ser Val Gly 435 440 445 Asp Ala Trp Asp His Gly Val Val Pro Tyr Thr Thr Ala Gly Ile Phe 450 455 460 Gly Lys Ser Ser Asn Val Gly Thr Leu Met Leu Ala Gln Arg Leu Gly 465 470 475 480 Glu Asp Lys Phe Ala Asp Tyr Leu Glu Arg Phe Gly Val Gly Gln Pro 485 490 495 Thr Gly Ile Glu Leu Pro Ser Glu Ser Gln Gly Leu Leu Pro Ala Arg 500 505 510 Glu Gln Trp Ser Gly Gly Thr Phe Ala Asn Leu Pro Ile Gly Gln Gly 515 520 525 Met Ser Ile Thr Thr Leu Gln Met Ala Gly Ile Tyr Gln Ala Leu Ala 530 535 540 Asn Asp Gly Glu Arg Ile Gln Pro Arg Ile Ile Lys Ser Val Thr Asp 545 550 555 560 Ser Asp Gly Thr Val Leu Glu Gln Pro Glu Pro Asp Lys Ile Gln Val 565 570 575 Val Ser Ala Glu Ala Ala Arg Thr Thr Val Val Asp Met Phe Arg Ser Val 580 585 590 Thr Gln Val Asp Pro Thr Gly Val Gln Gln Gly Thr Ala Pro Asp Ala 595 600 605 Ser Ile Glu Gly Tyr Gln Ile Ser Gly Lys Thr Gly Thr Ala Gln Lys 610 615 620 Val Asp Pro Asn Thr Gly Ala Tyr Ser Asn Ser Gln Tyr Trp Ile Thr 625 630 635 640 Phe Ala Gly Ile Ala Pro Ala Asp Asp Pro Arg Phe Val Val Ala Ile 645 650 655 Met Leu Asp Glu Pro Glu Arg Gly Val His Gly Gly Gly Gly Gln Thr 660 665 670 Ala Ala Pro Leu Phe Lys Asp Ile Ala Thr Trp Leu Leu Asn Arg Asp 675 680 685 Asn Ile Pro Leu Ser Ala Ala Thr Glu Pro Ile Ile Leu Gln Ala Gln 690 695 700 <210> 119 <211> 796 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (766) <223> RXA02723 <400> 119 cgtgctcacc atgggtgcag gttccgtgac catgcttgct ccagaaatcc tggatcagct 60 gcaaaacaat taggacgtaa gtgaacaagg caggactagc gtg aac aaa aaa gtc 115 Val Asn Lys Lys Val 1 5 atc gcc att gtt gtg ggt gtg gtt gtt gtc ctc gtg gca atc ctg ggc 163 Ile Ala Ile Val Val Gly Val Val Val Val Leu Val Ala Ile Leu Gly 10 15 20 gtt gtt gcc tgg ttc gtt ccc atc ctc aag gtg gga aac att gaa gta 211 Val Val Ala Trp Phe Val Pro Ile Leu Lys Val Gly Asn Ile Glu Val 25 30 35 acc ggt gca acg cgc aca gat ccg gat caa gta ctg gaa gtc tcc ggg 259 Thr Gly Ala Thr Arg Thr Asp Pro Asp Gln Val Leu Glu Val Ser Gly 40 45 50 att gtt gag ggc gaa aac ctc ttc cgc gtc gat gcg act gca gca ggg 307 Ile Val Glu Gly Glu Asn Leu Phe Arg Val Asp Ala Thr Ala Ala Gly 55 60 65 caa aac att gtg gaa ttg ccc tgg gtg aaa tcg gtg acc gtt aac cgt 355 Gln Asn Ile Val Glu Leu Pro Trp Val Lys Ser Val Thr Val Asn Arg 70 75 80 85 gcc ctg cca agc acc atc acc gtg gag ctg aca gag cgt gag cct gca 403 Ala Leu Pro Ser Thr Ile Thr Val Glu Leu Thr Glu Arg Glu Pro Ala 90 95 100 gtg ttc atc aag cgt gct gat ggt gac cat gtc att gac acc gag ggt 451 Val Phe Ile Lys Arg Ala Asp Gly Asp His Val Ile Asp Thr Glu Gly 105 110 115 aaa gaa att atc att gga aca ccc ccg gtg gga aca gta gaa gtt tct 499 Lys Glu Ile Ile Ile Gly Thr Pro Pro Val Gly Thr Val Glu Val Ser 120 125 130 ggc gcg gat gaa gga aac tca gaa gtg ctt cct gcg gtt att gct gta 547 Gly Ala Asp Glu Gly Asn Ser Glu Val Leu Pro Ala Val Ile Ala Val 135 140 145 atc aac gca att aaa gcg caa gat gcg cag atg aca gaa agt atc cag 595 Ile Asn Ala Ile Lys Ala Gln Asp Ala Gln Met Thr Glu Ser Ile Gln 150 155 160 165 gta gtg gaa gct ccg gat caa ttt gat atc ttg ctg aaa atg aat gat 643 Val Val Glu Ala Pro Asp Gln Phe Asp Ile Leu Leu Lys Met Asn Asp 170 175 180 ggc cgg gaa atc tac tgg gga tcc tcg gaa aac aac cac gat aaa gcg 691 Gly Arg Glu Ile Tyr Trp Gly Ser Ser Glu Asn Asn His Asp Lys Ala 185 190 195 gtg gca atg tcg act gtt ttg aag cgg gaa ggc caa cgt tgg aac att 739 Val Ala Met Ser Thr Val Leu Lys Arg Glu Gly Gln Arg Trp Asn Ile 200 205 210 agc tca ccc tca atg gtg aca gtc cgc taaagtggct gggtagttcc 786 Ser Ser Pro Ser Met Val Thr Val Arg 215 220 ggttcacccg 796 <210> 120 <211> 222 <212> PRT <213> Corynebacterium glutamicum <400> 120 Val Asn Lys Lys Val Ile Ala Ile Val Val Gly Val Val Val Val Leu 1 5 10 15 Val Ala Ile Leu Gly Val Val Ala Trp Phe Val Pro Ile Leu Lys Val 20 25 30 Gly Asn Ile Glu Val Thr Gly Ala Thr Arg Thr Asp Pro Asp Gln Val 35 40 45 Leu Glu Val Ser Gly Ile Val Glu Gly Glu Asn Leu Phe Arg Val Asp 50 55 60 Ala Thr Ala Ala Gly Gln Asn Ile Val Glu Leu Pro Trp Val Lys Ser 65 70 75 80 Val Thr Val Asn Arg Ala Leu Pro Ser Thr Ile Thr Val Glu Leu Thr 85 90 95 Glu Arg Glu Pro Ala Val Phe Ile Lys Arg Ala Asp Gly Asp His Val 100 105 110 Ile Asp Thr Glu Gly Lys Glu Ile Ile Ile Gly Thr Pro Pro Val Gly 115 120 125 Thr Val Glu Val Ser Gly Ala Asp Glu Gly Asn Ser Glu Val Leu Pro 130 135 140 Ala Val Ile Ala Val Ile Asn Ala Ile Lys Ala Gln Asp Ala Gln Met 145 150 155 160 Thr Glu Ser Ile Gln Val Val Glu Ala Pro Asp Gln Phe Asp Ile Leu 165 170 175 Leu Lys Met Asn Asp Gly Arg Glu Ile Tyr Trp Gly Ser Ser Glu Asn 180 185 190 Asn His Asp Lys Ala Val Ala Met Ser Thr Val Leu Lys Arg Glu Gly 195 200 205 Gln Arg Trp Asn Ile Ser Ser Pro Ser Met Val Thr Val Arg 210 215 220 <210> 121 <211> 712 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (682) <223> RXA03088 <400> 121 tcatggcacc gggacctggc atcgtagcta aggtttcttt tgatgatttc tccgacgtca 60 ccggcggcga tgaactcctc gaattggagg caaagaacta atg ggt caa acc cgc 115 Met Gly Gln Thr Arg 1 5 atc att tcc ggc gac gca cgc ggc cgc aag atc gaa gta cca cca gca 163 Ile Ile Ser Gly Asp Ala Arg Gly Arg Lys Ile Glu Val Pro Pro Ala 10 15 20 ggt acc cgc ccc acc tct gac cgc gca cgc gaa ggt ctc ttc tcc tca 211 Gly Thr Arg Pro Thr Ser Asp Arg Ala Arg Glu Gly Leu Phe Ser Ser 25 30 35 ctg cag gtc cgt ttc gga ttt gag ggc cag cgc gtc ctc gac att ttt 259 Leu Gln Val Arg Phe Gly Phe Glu Gly Gln Arg Val Leu Asp Ile Phe 40 45 50 gcc ggc tcc ggc gca ctc gga ttg gaa gct gcc tcc agg ggt gcc gat 307 Ala Gly Ser Gly Ala Leu Gly Leu Glu Ala Ala Ser Arg Gly Ala Asp 55 60 65 gag gta gtt ctg gtc gag tcg aat cct aag gcc gta gag gta att cga 355 Glu Val Val Leu Val Glu Ser Asn Pro Lys Ala Val Glu Val Ile Arg 70 75 80 85 cgg aat gtg gac gtc gta aag cat cct cgc gta acc gtc gca gag atg 403 Arg Asn Val Asp Val Val Lys His Pro Arg Val Thr Val Ala Glu Met 90 95 100 aaa gca tcc acc tac ctt gcg tcc gca ccc gat aag ttt ttc acg atg 451 Lys Ala Ser Thr Tyr Leu Ala Ser Ala Pro Asp Lys Phe Phe Thr Met 105 110 115 gtg ctc gcc gac ccg ccc tat gag ctt gcc gac gac gcc gtc acc gag 499 Val Leu Ala Asp Pro Pro Tyr Glu Leu Ala Asp Asp Ala Val Thr Glu 120 125 130 atg ctc acc gca ttg acg cct aag ctt ctc gac ggc gcc gcc gtg gtc 547 Met Leu Thr Ala Leu Thr Pro Lys Leu Leu Asp Gly Ala Ala Val Val 135 140 145 gtg gaa cgc cac gta gac tcg cca gaa acc gca tgg ccc gcc tgg ctg 595 Val Glu Arg His Val Asp Ser Pro Glu Thr Ala Trp Pro Ala Trp Leu 150 155 160 165 gta ccc acc acc cag aaa ctt aaa aaa cga acc tac ggc ata gca cgc 643 Val Pro Thr Thr Gln Lys Leu Lys Lys Arg Thr Tyr Gly Ile Ala Arg 170 175 180 atg gac atg gcc gtt ttc gac gaa tct ctg ctt cag gag taaccaatga 692 Met Asp Met Ala Val Phe Asp Glu Ser Leu Leu Gln Glu 185 190 aagctgtttg cccaggatca 712 <210> 122 <211> 194 <212> PRT <213> Corynebacterium glutamicum <400> 122 Met Gly Gln Thr Arg Ile Ile Ser Gly Asp Ala Arg Gly Arg Lys Ile 1 5 10 15 Glu Val Pro Pro Ala Gly Thr Arg Pro Thr Ser Asp Arg Ala Arg Glu 20 25 30 Gly Leu Phe Ser Ser Leu Gln Val Arg Phe Gly Phe Glu Gly Gln Arg 35 40 45 Val Leu Asp Ile Phe Ala Gly Ser Gly Ala Leu Gly Leu Glu Ala Ala 50 55 60 Ser Arg Gly Ala Asp Glu Val Val Leu Val Glu Ser Asn Pro Lys Ala 65 70 75 80 Val Glu Val Ile Arg Arg Asn Val Asp Val Val Lys His Pro Arg Val 85 90 95 Thr Val Ala Glu Met Lys Ala Ser Thr Tyr Leu Ala Ser Ala Pro Asp 100 105 110 Lys Phe Phe Thr Met Val Leu Ala Asp Pro Pro Tyr Glu Leu Ala Asp 115 120 125 Asp Ala Val Thr Glu Met Leu Thr Ala Leu Thr Pro Lys Leu Leu Asp 130 135 140 Gly Ala Ala Val Val Val Glu Arg His Val Asp Ser Pro Glu Thr Ala 145 150 155 160 Trp Pro Ala Trp Leu Val Pro Thr Thr Gln Lys Leu Lys Lys Arg Thr 165 170 175 Tyr Gly Ile Ala Arg Met Asp Met Ala Val Phe Asp Glu Ser Leu Leu 180 185 190 Gln glu <210> 123 <211> 1003 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (973) <223> RXA03128 <400> 123 cggaaggttc gacatctgca atgaaccttc aacttctggc caattggaca ggtaatgaca 60 gcccctccct cagcacagaa cttggtgaca ctatttaatt atg act ttc aca ctg 115 Met Thr Phe Thr Leu 1 5 aac aac gga ctc gcc att ccc gac att gga ttt ggt gta ttc caa acc 163 Asn Asn Gly Leu Ala Ile Pro Asp Ile Gly Phe Gly Val Phe Gln Thr 10 15 20 cca ccc gat gaa acc cga aac tcc gtt aac gct gct ctt gaa gcc ggc 211 Pro Pro Asp Glu Thr Arg Asn Ser Val Asn Ala Ala Leu Glu Ala Gly 25 30 35 tat cgc cac atc gac acc gcc gcc gca tac ggc aat gaa cgt gaa gtc 259 Tyr Arg His Ile Asp Thr Ala Ala Ala Tyr Gly Asn Glu Arg Glu Val 40 45 50 ggt gaa gca atc gca gca tcc ggc att ggc cgc gac gag atc acc atc 307 Gly Glu Ala Ile Ala Ala Ser Gly Ile Gly Arg Asp Glu Ile Thr Ile 55 60 65 gaa acc aaa atc tgg gtg acc gac tac ggc ttc gag gaa act ctc cac 355 Glu Thr Lys Ile Trp Val Thr Asp Tyr Gly Phe Glu Glu Thr Leu His 70 75 80 85 gca ttc gac aag gcc aca ggc aag ctt ggt gtc gat aca ctg gac att 403 Ala Phe Asp Lys Ala Thr Gly Lys Leu Gly Val Asp Thr Leu Asp Ile 90 95 100 ttg atc ttg cac cag gca gtg cca agc agc ttt gat cgc acc atc gcc 451 Leu Ile Leu His Gln Ala Val Pro Ser Ser Phe Asp Arg Thr Ile Ala 105 110 115 gcc tac aag gcg cta gag aag ctg ctt ttc gac ggc gcg gtg cgg gca 499 Ala Tyr Lys Ala Leu Glu Lys Leu Leu Phe Asp Gly Ala Val Arg Ala 120 125 130 atc gga gtc agt aat ttc atg cca gag cac ctg gac aaa ctc ctt ttg 547 Ile Gly Val Ser Asn Phe Met Pro Glu His Leu Asp Lys Leu Leu Leu 135 140 145 gaa acc tcc att gtc cca gct ctg aac caa atc gaa tgc cac ccc tac 595 Glu Thr Ser Ile Val Pro Ala Leu Asn Gln Ile Glu Cys His Pro Tyr 150 155 160 165 ttc cag cag cgt gac gtg ctt gcc cgc aat gag cag ctt ggc att ttg 643 Phe Gln Gln Arg Asp Val Leu Ala Arg Asn Glu Gln Leu Gly Ile Leu 170 175 180 act cag gcg tgg tca cca atc ggt ggc atc acc ttc tac cgc gac gga 691 Thr Gln Ala Trp Ser Pro Ile Gly Gly Ile Thr Phe Tyr Arg Asp Gly 185 190 195 cag ctt cca agc act cta gaa aat gag gtc atc gct gga atc gcc gca 739 Gln Leu Pro Ser Thr Leu Glu Asn Glu Val Ile Ala Gly Ile Ala Ala 200 205 210 gaa gtt ggc aaa aca cca gct caa gta atg ctg cgc tgg cac cta cag 787 Glu Val Gly Lys Thr Pro Ala Gln Val Met Leu Arg Trp His Leu Gln 215 220 225 cgt aga cgt cac gca att cca aag tct gtg acc cca tca cgc att gtg 835 Arg Arg Arg His Ala Ile Pro Lys Ser Val Thr Pro Ser Arg Ile Val 230 235 240 245 gaa aac ttt gag atc ttt gat ttc gaa ctc tcc gat gag caa cta cag 883 Glu Asn Phe Glu Ile Phe Asp Phe Glu Leu Ser Asp Glu Gln Leu Gln 250 255 260 caa atc gat gcc ctc aac acc gat ctg cgc ggt ggc cca gaa cca gag 931 Gln Ile Asp Ala Leu Asn Thr Asp Leu Arg Gly Gly Pro Glu Pro Glu 265 270 275 aac atc acc atg gaa aac tac tac cga gaa atc cca gaa gcc 973 Asn Ile Thr Met Glu Asn Tyr Tyr Arg Glu Ile Pro Glu Ala 280 285 290 taaaggccct tagaggcgaa tgtaggccta 1003 <210> 124 <211> 291 <212> PRT <213> Corynebacterium glutamicum <400> 124 Met Thr Phe Thr Leu Asn Asn Gly Leu Ala Ile Pro Asp Ile Gly Phe 1 5 10 15 Gly Val Phe Gln Thr Pro Pro Asp Glu Thr Arg Asn Ser Val Asn Ala 20 25 30 Ala Leu Glu Ala Gly Tyr Arg His Ile Asp Thr Ala Ala Ala Tyr Gly 35 40 45 Asn Glu Arg Glu Val Gly Glu Ala Ile Ala Ala Ser Gly Ile Gly Arg 50 55 60 Asp Glu Ile Thr Ile Glu Thr Lys Ile Trp Val Thr Asp Tyr Gly Phe 65 70 75 80 Glu Glu Thr Leu His Ala Phe Asp Lys Ala Thr Gly Lys Leu Gly Val 85 90 95 Asp Thr Leu Asp Ile Leu Ile Leu His Gln Ala Val Pro Ser Ser Phe 100 105 110 Asp Arg Thr Ile Ala Ala Tyr Lys Ala Leu Glu Lys Leu Leu Phe Asp 115 120 125 Gly Ala Val Arg Ala Ile Gly Val Ser Asn Phe Met Pro Glu His Leu 130 135 140 Asp Lys Leu Leu Leu Glu Thr Ser Ile Val Pro Ala Leu Asn Gln Ile 145 150 155 160 Glu Cys His Pro Tyr Phe Gln Gln Arg Asp Val Leu Ala Arg Asn Glu 165 170 175 Gln Leu Gly Ile Leu Thr Gln Ala Trp Ser Pro Ile Gly Gly Ile Thr 180 185 190 Phe Tyr Arg Asp Gly Gln Leu Pro Ser Thr Leu Glu Asn Glu Val Ile 195 200 205 Ala Gly Ile Ala Ala Glu Val Gly Lys Thr Pro Ala Gln Val Met Leu 210 215 220 Arg Trp His Leu Gln Arg Arg Arg His Ala Ile Pro Lys Ser Val Thr 225 230 235 240 Pro Ser Arg Ile Val Glu Asn Phe Glu Ile Phe Asp Phe Glu Leu Ser 245 250 255 Asp Glu Gln Leu Gln Gln Ile Asp Ala Leu Asn Thr Asp Leu Arg Gly 260 265 270 Gly Pro Glu Pro Glu Asn Ile Thr Met Glu Asn Tyr Tyr Arg Glu Ile 275 280 285 Pro Glu Ala 290 <210> 125 <211> 919 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (889) <223> RXA03133 <400> 125 tacgccaccg ccagcgcctg gccccactac cccattgccc acgcagccca ggccgcggtg 60 caattagcca ggccaatgcg ggtttttgag ctccagagct ttg aag gcg tca agc 115 Leu Lys Ala Ser Ser 1 5 acg cac tcc acc aca tcg atc ttc gcc ccg ccc tcg aat ggg aca tca 163 Thr His Ser Thr Thr Ser Ile Phe Ala Pro Pro Ser Asn Gly Thr Ser 10 15 20 tgg gat ttc ccg aat ccc ccg aca cgc tgc cca ttt tgc tca gtg acc 211 Trp Asp Phe Pro Asn Pro Pro Thr Arg Cys Pro Phe Cys Ser Val Thr 25 30 35 tgc gcg acc ccc ctt acg cca cca ctg ctg tgc cac ctc aat cca cgc 259 Cys Ala Thr Pro Leu Thr Pro Pro Leu Leu Cys His Leu Asn Pro Arg 40 45 50 cac gct ccg ctc acc gat cca cac att gac gct tcc agg cgc cac cgc 307 His Ala Pro Leu Thr Asp Pro His Ile Asp Ala Ser Arg Arg His Arg 55 60 65 ggt ccc ttc cac gcc act ccc agc tac gca ggc ttt cac ccc tca ttt 355 Gly Pro Phe His Ala Thr Pro Ser Tyr Ala Gly Phe His Pro Ser Phe 70 75 80 85 acc gac gac acc gcc cgc atg ctg ggc aac cta gcc aaa att ggc gcg 403 Thr Asp Asp Thr Ala Arg Met Leu Gly Asn Leu Ala Lys Ile Gly Ala 90 95 100 gtt gcc acc tgg gaa aag acg ctt gtc gac gcc gca agc tgg gcc gaa 451 Val Ala Thr Trp Glu Lys Thr Leu Val Asp Ala Ala Ser Trp Ala Glu 105 110 115 ctc ccc cac cca cca gtc atc gtc ggg gat acc ccg ccc tcg act gtc 499 Leu Pro His Pro Pro Val Ile Val Gly Asp Thr Pro Pro Ser Thr Val 120 125 130 acg ccg gac aac gcc tgg tac cag ttt cgc gtc ggc aag cat ctt ggt 547 Thr Pro Asp Asn Ala Trp Tyr Gln Phe Arg Val Gly Lys His Leu Gly 135 140 145 cgc gat gcg ttc gcg gaa atc gtc atg tgc ctt ggc cac gtg ttt tgc 595 Arg Asp Ala Phe Ala Glu Ile Val Met Cys Leu Gly His Val Phe Cys 150 155 160 165 cat cat gtc ccc cag gta tgg tcg aca acc tgc gca tct cag gac gct 643 His His Val Pro Gln Val Trp Ser Thr Thr Cys Ala Ser Gln Asp Ala 170 175 180 gat tcc acc acg atg ctc att gaa gcc gag acc gcg ggg gca ttg gcc 691 Asp Ser Thr Thr Met Leu Ile Glu Ala Glu Thr Ala Gly Ala Leu Ala 185 190 195 atc gct cgc gtc ggc ggc ccg acc cgt cga ggt ggt tcc ttc ttc ggc 739 Ile Ala Arg Val Gly Gly Pro Thr Arg Arg Gly Gly Ser Phe Phe Gly 200 205 210 gat gcc ctt ctc aaa gag ggg acg cca ctt cct gct ggt ttc cga cta 787 Asp Ala Leu Leu Lys Glu Gly Thr Pro Leu Pro Ala Gly Phe Arg Leu 215 220 225 gat gtt gtt cta cac gcc gcc tca gaa att gag gat ctg ctg cgc ggt 835 Asp Val Val Leu His Ala Ala Ser Glu Ile Glu Asp Leu Leu Arg Gly 230 235 240 245 gac acc aca gcc gtt gtc agc ggc gct tgg tct gtg gag gat cgc cga 883 Asp Thr Thr Ala Val Val Ser Gly Ala Trp Ser Val Glu Asp Arg Arg 250 255 260 ggt tac taacaaatag gcccaacaaa gaggtctaag 919 Gly tyr <210> 126 <211> 263 <212> PRT <213> Corynebacterium glutamicum <400> 126 Leu Lys Ala Ser Ser Thr His Ser Thr Thr Ser Ile Phe Ala Pro Pro 1 5 10 15 Ser Asn Gly Thr Ser Trp Asp Phe Pro Asn Pro Pro Thr Arg Cys Pro 20 25 30 Phe Cys Ser Val Thr Cys Ala Thr Pro Leu Thr Pro Pro Leu Leu Cys 35 40 45 His Leu Asn Pro Arg His Ala Pro Leu Thr Asp Pro His Ile Asp Ala 50 55 60 Ser Arg Arg His Arg Gly Pro Phe His Ala Thr Pro Ser Tyr Ala Gly 65 70 75 80 Phe His Pro Ser Phe Thr Asp Asp Thr Ala Arg Met Leu Gly Asn Leu 85 90 95 Ala Lys Ile Gly Ala Val Ala Thr Trp Glu Lys Thr Leu Val Asp Ala 100 105 110 Ala Ser Trp Ala Glu Leu Pro His Pro Pro Val Ile Val Gly Asp Thr 115 120 125 Pro Pro Ser Thr Val Thr Pro Asp Asn Ala Trp Tyr Gln Phe Arg Val 130 135 140 Gly Lys His Leu Gly Arg Asp Ala Phe Ala Glu Ile Val Met Cys Leu 145 150 155 160 Gly His Val Phe Cys His His Val Pro Gln Val Trp Ser Thr Thr Cys 165 170 175 Ala Ser Gln Asp Ala Asp Ser Thr Thr Met Leu Ile Glu Ala Glu Thr 180 185 190 Ala Gly Ala Leu Ala Ile Ala Arg Val Gly Gly Pro Thr Arg Arg Gly 195 200 205 Gly Ser Phe Phe Gly Asp Ala Leu Leu Lys Glu Gly Thr Pro Leu Pro 210 215 220 Ala Gly Phe Arg Leu Asp Val Val Leu His Ala Ala Ser Glu Ile Glu 225 230 235 240 Asp Leu Leu Arg Gly Asp Thr Thr Ala Val Val Ser Gly Ala Trp Ser 245 250 255 Val Glu Asp Arg Arg Gly Tyr 260 <210> 127 <211> 919 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (889) <223> RXA03150 <400> 127 acacttcttc gcgggataca gtcatgacag tgcaatctca ctgtccatag aaatgtaact 60 catatggaat cgcagtatca taaattgcaa tctccccggg ttg cag tcg caa atc 115 Leu Gln Ser Gln Ile 1 5 tgg ctc tcc atc ccg ggt gag cac cat ggt ctc gga ttt cag tgc aca 163 Trp Leu Ser Ile Pro Gly Glu His His Gly Leu Gly Phe Gln Cys Thr 10 15 20 tac ttt aac aga gtg cgt ttc aat gcc tgt agt gtt ccg gca att ttg 211 Tyr Phe Asn Arg Val Arg Phe Asn Ala Cys Ser Val Pro Ala Ile Leu 25 30 35 aat gtc gtt acg tta ccc aag gct gaa ttc ctg agc tca cct tgt aca 259 Asn Val Val Thr Leu Pro Lys Ala Glu Phe Leu Ser Ser Pro Cys Thr 40 45 50 aga tca gtg gaa gcc cag ttc acc tct ccc ctg ctc aac aat ggg caa 307 Arg Ser Val Glu Ala Gln Phe Thr Ser Pro Leu Leu Asn Asn Gly Gln 55 60 65 acc tgt ttc ctt ggt acc cga atc ctt gct cca aaa tca cgt tac gcg 355 Thr Cys Phe Leu Gly Thr Arg Ile Leu Ala Pro Lys Ser Arg Tyr Ala 70 75 80 85 gaa gta gtc gat gca ttc acc gct ttc gct ggc agc ctg cag gtt gga 403 Glu Val Val Asp Ala Phe Thr Ala Phe Ala Gly Ser Leu Gln Val Gly 90 95 100 gtc acg tcc tcc cct gac act cag atc gga ccg atg gcg act gcc cgg 451 Val Thr Ser Ser Pro Asp Thr Gln Ile Gly Pro Met Ala Thr Ala Arg 105 110 115 cag cgt gag cgc gtg gaa tcc tac att tcc caa ggc aaa aat gct gga 499 Gln Arg Glu Arg Val Glu Ser Tyr Ile Ser Gln Gly Lys Asn Ala Gly 120 125 130 gcc cgc atc act gtc ggt ggc agc cgt cca cga gat ctt gac gcc gga 547 Ala Arg Ile Thr Val Gly Gly Ser Arg Pro Arg Asp Leu Asp Ala Gly 135 140 145 ttc ttc gtt gag cca aca gtg ttc gcc gat gta gac aat cgc gca gcc 595 Phe Phe Val Glu Pro Thr Val Phe Ala Asp Val Asp Asn Arg Ala Ala 150 155 160 165 att gcc caa gat gaa atc ttc gga ccg gtg ccc tct gtt gtt tcc tac 643 Ile Ala Gln Asp Glu Ile Phe Gly Pro Val Pro Ser Val Val Ser Tyr 170 175 180 caa gac gat gaa cac gcc atc caa cta gcc aac gat tcc gaa ttc ggt 691 Gln Asp Asp Glu His Ala Ile Gln Leu Ala Asn Asp Ser Glu Phe Gly 185 190 195 ctc ggc gga act gtc tgg acg agc gat ccc gag cgc ggc gct gca ttg 739 Leu Gly Gly Thr Val Trp Thr Ser Asp Pro Glu Arg Gly Ala Ala Leu 200 205 210 gcc cgc cga gtt cac aca gga acc att ggc atc aac cgc tat atc cct 787 Ala Arg Arg Val His Thr Gly Thr Ile Gly Ile Asn Arg Tyr Ile Pro 215 220 225 gat ccc gcc gca cca ttt gga ggt gtg aaa aac agt ggc ctt ggc aga 835 Asp Pro Ala Ala Pro Phe Gly Gly Val Lys Asn Ser Gly Leu Gly Arg 230 235 240 245 gaa ctc ggc ccc gaa ggt ctt gct tcc tac caa gaa acc caa acc att 883 Glu Leu Gly Pro Glu Gly Leu Ala Ser Tyr Gln Glu Thr Gln Thr Ile 250 255 260 tat ctc taatccaaac tgcacctata tataaggagt 919 Tyr leu <210> 128 <211> 263 <212> PRT <213> Corynebacterium glutamicum <400> 128 Leu Gln Ser Gln Ile Trp Leu Ser Ile Pro Gly Glu His His Gly Leu 1 5 10 15 Gly Phe Gln Cys Thr Tyr Phe Asn Arg Val Arg Phe Asn Ala Cys Ser 20 25 30 Val Pro Ala Ile Leu Asn Val Val Thr Leu Pro Lys Ala Glu Phe Leu 35 40 45 Ser Ser Pro Cys Thr Arg Ser Val Glu Ala Gln Phe Thr Ser Pro Leu 50 55 60 Leu Asn Asn Gly Gln Thr Cys Phe Leu Gly Thr Arg Ile Leu Ala Pro 65 70 75 80 Lys Ser Arg Tyr Ala Glu Val Val Asp Ala Phe Thr Ala Phe Ala Gly 85 90 95 Ser Leu Gln Val Gly Val Thr Ser Ser Pro Asp Thr Gln Ile Gly Pro 100 105 110 Met Ala Thr Ala Arg Gln Arg Glu Arg Val Glu Ser Tyr Ile Ser Gln 115 120 125 Gly Lys Asn Ala Gly Ala Arg Ile Thr Val Gly Gly Ser Arg Pro Arg 130 135 140 Asp Leu Asp Ala Gly Phe Phe Val Glu Pro Thr Val Phe Ala Asp Val 145 150 155 160 Asp Asn Arg Ala Ala Ile Ala Gln Asp Glu Ile Phe Gly Pro Val Pro 165 170 175 Ser Val Val Ser Tyr Gln Asp Asp Glu His Ala Ile Gln Leu Ala Asn 180 185 190 Asp Ser Glu Phe Gly Leu Gly Gly Thr Val Trp Thr Ser Asp Pro Glu 195 200 205 Arg Gly Ala Ala Leu Ala Arg Arg Val His Thr Gly Thr Ile Gly Ile 210 215 220 Asn Arg Tyr Ile Pro Asp Pro Ala Ala Pro Phe Gly Gly Val Lys Asn 225 230 235 240 Ser Gly Leu Gly Arg Glu Leu Gly Pro Glu Gly Leu Ala Ser Tyr Gln 245 250 255 Glu Thr Gln Thr Ile Tyr Leu 260 <210> 129 <211> 451 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (421) <223> RXA03369 <400> 129 gtctcaagct tctcaaggcc ggagagggtg gaagcctcga agttatttgg ggtaacgacg 60 gttgcttgag gcagaagttc taccccttga tctttccccg ttg tgg aat aaa gtt 115 Leu Trp Asn Lys Val 1 5 cac acc atc gta tct tca atc cct gca ggt cat tgg gcg agt tac gga 163 His Thr Ile Val Ser Ser Ile Pro Ala Gly His Trp Ala Ser Tyr Gly 10 15 20 gat gtt gca gtg gca gcc gga aca aat gct cag tcc att ggt aac cat 211 Asp Val Ala Val Ala Ala Gly Thr Asn Ala Gln Ser Ile Gly Asn His 25 30 35 ctt gct gaa cac cca gta cta aac gct cat cga gtt cta aga aca gat 259 Leu Ala Glu His Pro Val Leu Asn Ala His Arg Val Leu Arg Thr Asp 40 45 50 ggg tca atc tca gat ggt ttc aga tgg tta aac gct gag gaa acc cgc 307 Gly Ser Ile Ser Asp Gly Phe Arg Trp Leu Asn Ala Glu Glu Thr Arg 55 60 65 tca cca aaa gaa gta ttg gaa agt gaa ggt gtg gaa ttt act tct tcc 355 Ser Pro Lys Glu Val Leu Glu Ser Glu Gly Val Glu Phe Thr Ser Ser 70 75 80 85 aat agg gca aac cct caa cag cga ttg act cca gag gaa ttg ctc gcc 403 Asn Arg Ala Asn Pro Gln Gln Arg Leu Thr Pro Glu Glu Leu Leu Ala 90 95 100 ttc ttt gca gct gag aat taactcaccc ttgcccttat accccatagg 451 Phe Phe Ala Ala Glu Asn 105 <210> 130 <211> 107 <212> PRT <213> Corynebacterium glutamicum <400> 130 Leu Trp Asn Lys Val His Thr Ile Val Ser Ser Ile Pro Ala Gly His 1 5 10 15 Trp Ala Ser Tyr Gly Asp Val Ala Val Ala Ala Gly Thr Asn Ala Gln 20 25 30 Ser Ile Gly Asn His Leu Ala Glu His Pro Val Leu Asn Ala His Arg 35 40 45 Val Leu Arg Thr Asp Gly Ser Ile Ser Asp Gly Phe Arg Trp Leu Asn 50 55 60 Ala Glu Glu Thr Arg Ser Pro Lys Glu Val Leu Glu Ser Glu Gly Val 65 70 75 80 Glu Phe Thr Ser Ser Asn Arg Ala Asn Pro Gln Gln Arg Leu Thr Pro 85 90 95 Glu Glu Leu Leu Ala Phe Phe Ala Ala Glu Asn 100 105 <210> 131 <211> 1549 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1519) <223> RXA03433 <400> 131 aaactctttg gttttcatac ctttcttgct tgattttcat gaaaagcaca ttcccagtga 60 agccctgaag cacactctcg agcccaacat aaggaataaa atg tct ttc aag cgt 115 Met Ser Phe Lys Arg 1 5 cgt tcg ctt gca atc act tgc gcc atc acc tcc agc att gtt ctc gct 163 Arg Ser Leu Ala Ile Thr Cys Ala Ile Thr Ser Ser Ile Val Leu Ala 10 15 20 gga atc tcc ggc acc gct gcc gcc cag tcc tcc tac gtt tct gga tcc 211 Gly Ile Ser Gly Thr Ala Ala Ala Gln Ser Ser Tyr Val Ser Gly Ser 25 30 35 tca gga ctg ctg tcc tcc agc gag ctc cat cct gaa act cca gca cct 259 Ser Gly Leu Leu Ser Ser Ser Glu Leu His Pro Glu Thr Pro Ala Pro 40 45 50 ctg gat gct cca aag aac atc atc tac atg atc ggt gac ggc atg ggt 307 Leu Asp Ala Pro Lys Asn Ile Ile Tyr Met Ile Gly Asp Gly Met Gly 55 60 65 tac aac cat gtc tcc gca acc aac ctg ttt gaa acc ggc cag acc atg 355 Tyr Asn His Val Ser Ala Thr Asn Leu Phe Glu Thr Gly Gln Thr Met 70 75 80 85 cac cag gtc gaa ggc gag cca ggt tct gtc acc cca gtt gag ggt ggc 403 His Gln Val Glu Gly Glu Pro Gly Ser Val Thr Pro Val Glu Gly Gly 90 95 100 act cca gtt cag gca ttt gaa ggc gct gag tgg acc cag ctt gca cag 451 Thr Pro Val Gln Ala Phe Glu Gly Ala Glu Trp Thr Gln Leu Ala Gln 105 110 115 tcc acc ttc cag gat gga aac tcc tac gac cct gag cgc gcg tgg gct 499 Ser Thr Phe Gln Asp Gly Asn Ser Tyr Asp Pro Glu Arg Ala Trp Ala 120 125 130 gat cac aac tat gtc aat gag aac ttc acc gac tcc gct gct gca gga 547 Asp His Asn Tyr Val Asn Glu Asn Phe Thr Asp Ser Ala Ala Ala Gly 135 140 145 acc gca atg gca acc ggc gtt aag acc acc aac ggc atg atc ggc atc 595 Thr Ala Met Ala Thr Gly Val Lys Thr Thr Asn Gly Met Ile Gly Ile 150 155 160 165 aac cca gcc aac gag cct gcg aag aac acc tct gag tac gca att gaa 643 Asn Pro Ala Asn Glu Pro Ala Lys Asn Thr Ser Glu Tyr Ala Ile Glu 170 175 180 aag ggc aag gca gcc ggc gtt gtt tcc tct gtt cct ttc aac cac gca 691 Lys Gly Lys Ala Ala Gly Val Val Ser Ser Val Pro Phe Asn His Ala 185 190 195 act cct gcg gct tgg gca gct cac aac tcc aac cgc aat gat ctg cac 739 Thr Pro Ala Ala Trp Ala Ala His Asn Ser Asn Arg Asn Asp Leu His 200 205 210 gcg atg gcc gaa gag atg atc aac agt gat ctc aac gtc atc atg ggt 787 Ala Met Ala Glu Glu Met Ile Asn Ser Asp Leu Asn Val Ile Met Gly 215 220 225 gct ggc cac cca ttc ttc gac aac aac ggc aac cca atc aca gaa gcc 835 Ala Gly His Pro Phe Phe Asp Asn Asn Gly Asn Pro Ile Thr Glu Ala 230 235 240 245 gac gaa gac tac atg cag gct tcc cag tac gag cgc ctc gca agt ggc 883 Asp Glu Asp Tyr Met Gln Ala Ser Gln Tyr Glu Arg Leu Ala Ser Gly 250 255 260 gaa acc gac ttc acc ttc gtc gaa gag gac gca gac ttc gag gct ctc 931 Glu Thr Asp Phe Thr Phe Val Glu Glu Asp Ala Asp Phe Glu Ala Leu 265 270 275 gca aac ggc caa gtt gaa agt gac aag tac ttc ggt ctc gcg cag gtc 979 Ala Asn Gly Gln Val Glu Ser Asp Lys Tyr Phe Gly Leu Ala Gln Val 280 285 290 gaa gac ccc ctt cag cac gac cgc gat ggc gac tct gtc act cct tac 1027 Glu Asp Pro Leu Gln His Asp Arg Asp Gly Asp Ser Val Thr Pro Tyr 295 300 305 gac gtt cca ctc aac gac gtc gtc gac ctt tcc acc atg tcc aag gca 1075 Asp Val Pro Leu Asn Asp Val Val Asp Leu Ser Thr Met Ser Lys Ala 310 315 320 325 gcc ctc aac gtt ctt aac cag gac gaa gac gga ttc cac atc atg atc 1123 Ala Leu Asn Val Leu Asn Gln Asp Glu Asp Gly Phe His Ile Met Ile 330 335 340 gaa ggt ggc gca atc gac tgg gca ggc cac gca aat gac atg gct cgc 1171 Glu Gly Gly Ala Ile Asp Trp Ala Gly His Ala Asn Asp Met Ala Arg 345 350 355 gac att gaa gaa gtc caa gag ttc aac aag gcc gtt gaa act gtc atc 1219 Asp Ile Glu Glu Val Gln Glu Phe Asn Lys Ala Val Glu Thr Val Ile 360 365 370 gag tgg gtt gag acc aac tcc tct tgg gat gaa acc ctc gtg atc gtc 1267 Glu Trp Val Glu Thr Asn Ser Ser Trp Asp Glu Thr Leu Val Ile Val 375 380 385 acc gcc gac cac gaa acc ggc tac atg acc ggc cct aac gat gat cca 1315 Thr Ala Asp His Glu Thr Gly Tyr Met Thr Gly Pro Asn Asp Asp Pro 390 395 400 405 aac tgg tcc gca atg acc ggc gct gca ggc atc gtt cca aac cac ggc 1363 Asn Trp Ser Ala Met Thr Gly Ala Ala Gly Ile Val Pro Asn His Gly 410 415 420 tgg cac tcc ggt aac cac atc aac cag ctc gtc cca gtg ttc gct cgt 1411 Trp His Ser Gly Asn His Ile Asn Gln Leu Val Pro Val Phe Ala Arg 425 430 435 ggc gca ggt gtc tcc gat atc gtt gct gct gcc gat caa gtt gac cca 1459 Gly Ala Gly Val Ser Asp Ile Val Ala Ala Ala Asp Gln Val Asp Pro 440 445 450 att cgc ggg aat tac atc gac aac att gag atc gcg aac ctg act ttc 1507 Ile Arg Gly Asn Tyr Ile Asp Asn Ile Glu Ile Ala Asn Leu Thr Phe 455 460 465 aac aag tgg tgg taacccccgc ttgaccagcc gttttggtta 1549 Asn Lys Trp Trp 470 <210> 132 <211> 473 <212> PRT <213> Corynebacterium glutamicum <400> 132 Met Ser Phe Lys Arg Arg Ser Leu Ala Ile Thr Cys Ala Ile Thr Ser 1 5 10 15 Ser Ile Val Leu Ala Gly Ile Ser Gly Thr Ala Ala Ala Gln Ser Ser 20 25 30 Tyr Val Ser Gly Ser Ser Gly Leu Leu Ser Ser Ser Glu Leu His Pro 35 40 45 Glu Thr Pro Ala Pro Leu Asp Ala Pro Lys Asn Ile Tyr Met Ile 50 55 60 Gly Asp Gly Met Gly Tyr Asn His Val Ser Ala Thr Asn Leu Phe Glu 65 70 75 80 Thr Gly Gln Thr Met His Gln Val Glu Gly Glu Pro Gly Ser Val Thr 85 90 95 Pro Val Glu Gly Gly Thr Pro Val Gln Ala Phe Glu Gly Ala Glu Trp 100 105 110 Thr Gln Leu Ala Gln Ser Thr Phe Gln Asp Gly Asn Ser Tyr Asp Pro 115 120 125 Glu Arg Ala Trp Ala Asp His Asn Tyr Val Asn Glu Asn Phe Thr Asp 130 135 140 Ser Ala Ala Ala Gly Thr Ala Met Ala Thr Gly Val Lys Thr Thr Asn 145 150 155 160 Gly Met Ile Gly Ile Asn Pro Ala Asn Glu Pro Ala Lys Asn Thr Ser 165 170 175 Glu Tyr Ala Ile Glu Lys Gly Lys Ala Ala Gly Val Val Ser Ser Val 180 185 190 Pro Phe Asn His Ala Thr Pro Ala Ala Trp Ala Ala His Asn Ser Asn 195 200 205 Arg Asn Asp Leu His Ala Met Ala Glu Glu Met Ile Asn Ser Asp Leu 210 215 220 Asn Val Ile Met Gly Ala Gly His Pro Phe Phe Asp Asn Asn Gly Asn 225 230 235 240 Pro Ile Thr Glu Ala Asp Glu Asp Tyr Met Gln Ala Ser Gln Tyr Glu 245 250 255 Arg Leu Ala Ser Gly Glu Thr Asp Phe Thr Phe Val Glu Glu Asp Ala 260 265 270 Asp Phe Glu Ala Leu Ala Asn Gly Gln Val Glu Ser Asp Lys Tyr Phe 275 280 285 Gly Leu Ala Gln Val Glu Asp Pro Leu Gln His Asp Arg Asp Gly Asp 290 295 300 Ser Val Thr Pro Tyr Asp Val Pro Leu Asn Asp Val Val Asp Leu Ser 305 310 315 320 Thr Met Ser Lys Ala Ala Leu Asn Val Leu Asn Gln Asp Glu Asp Gly 325 330 335 Phe His Ile Met Ile Glu Gly Gly Ala Ile Asp Trp Ala Gly His Ala 340 345 350 Asn Asp Met Ala Arg Asp Ile Glu Glu Val Gln Glu Phe Asn Lys Ala 355 360 365 Val Glu Thr Val Ile Glu Trp Val Glu Thr Asn Ser Ser Trp Asp Glu 370 375 380 Thr Leu Val Ile Val Thr Ala Asp His Glu Thr Gly Tyr Met Thr Gly 385 390 395 400 Pro Asn Asp Asp Pro Asn Trp Ser Ala Met Thr Gly Ala Ala Gly Ile 405 410 415 Val Pro Asn His Gly Trp His Ser Gly Asn His Ile Asn Gln Leu Val 420 425 430 Pro Val Phe Ala Arg Gly Ala Gly Val Ser Asp Ile Val Ala Ala Ala 435 440 445 Asp Gln Val Asp Pro Ile Arg Gly Asn Tyr Ile Asp Asn Ile Glu Ile 450 455 460 Ala Asn Leu Thr Phe Asn Lys Trp Trp 465 470 <210> 133 <211> 577 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .. (547) <223> RXA03501 <133> 133 tcgacgcgcc cctccatcac agtcatcccg ccgcaggcgg gcgtcgaaaa gcggcgacct 60 gcggatctgt gacccgcgcc ataaatgggt acgatggcgg ttg gaa aat tcc gta 115 Leu Glu Asn Ser Val 1 5 act tct ttg agg gag aaa ccc atg gtc ggc acg att tcc acc tac atc 163 Thr Ser Leu Arg Glu Lys Pro Met Val Gly Thr Ile Ser Thr Tyr Ile 10 15 20 gca ttc aat gga aac acc acg gaa gct ctg aaa cat tgg caa gag gtt 211 Ala Phe Asn Gly Asn Thr Thr Glu Ala Leu Lys His Trp Gln Glu Val 25 30 35 ttt ggt ggt gaa ctt aat ctt tta acc tac ggt cag ctc acc ttg gaa 259 Phe Gly Gly Glu Leu Asn Leu Leu Thr Tyr Gly Gln Leu Thr Leu Glu 40 45 50 ggt atg ccg ttt gat cct cca gcg gat gcg ttg gcg cac ggc gtc ctc 307 Gly Met Pro Phe Asp Pro Pro Ala Asp Ala Leu Ala His Gly Val Leu 55 60 65 acg ttg gac aat ggt ggt ttg att tct ggc agt gat tct ttt gag gga 355 Thr Leu Asp Asn Gly Gly Leu Ile Ser Gly Ser Asp Ser Phe Glu Gly 70 75 80 85 gaa atg ctc gtc aaa gac acc gcg tac tcc atg ttg tat aac gcg gag 403 Glu Met Leu Val Lys Asp Thr Ala Tyr Ser Met Leu Tyr Asn Ala Glu 90 95 100 tca gtg gaa gat ggt cgc gcg cgg atc gac aag att gtg gcg gct ggc 451 Ser Val Glu Asp Gly Arg Ala Arg Ile Asp Lys Ile Val Ala Ala Gly 105 110 115 ggt gag gaa gcc atg aag ttt gaa cag gct ccg tgg ggc ggt ttc ttt 499 Gly Glu Glu Ala Met Lys Phe Glu Gln Ala Pro Trp Gly Gly Phe Phe 120 125 130 ggc cag gtt ttt gat aaa ttt ggt gtg atg tgg aat att tcc tca gac 547 Gly Gln Val Phe Asp Lys Phe Gly Val Met Trp Asn Ile Ser Ser Asp 135 140 145 taaaagcata aaaagatcgt tgcattttca 577 <210> 134 <211> 149 <212> PRT <213> Corynebacterium glutamicum <400> 134 Leu Glu Asn Ser Val Thr Ser Leu Arg Glu Lys Pro Met Val Gly Thr 1 5 10 15 Ile Ser Thr Tyr Ile Ala Phe Asn Gly Asn Thr Thr Glu Ala Leu Lys 20 25 30 His Trp Gln Glu Val Phe Gly Gly Glu Leu Asn Leu Leu Thr Tyr Gly 35 40 45 Gln Leu Thr Leu Glu Gly Met Pro Phe Asp Pro Pro Ala Asp Ala Leu 50 55 60 Ala His Gly Val Leu Thr Leu Asp Asn Gly Gly Leu Ile Ser Gly Ser 65 70 75 80 Asp Ser Phe Glu Gly Glu Met Leu Val Lys Asp Thr Ala Tyr Ser Met 85 90 95 Leu Tyr Asn Ala Glu Ser Val Glu Asp Gly Arg Ala Arg Ile Asp Lys 100 105 110 Ile Val Ala Ala Gly Gly Glu Glu Ala Met Lys Phe Glu Gln Ala Pro 115 120 125 Trp Gly Gly Phe Phe Gly Gln Val Phe Asp Lys Phe Gly Val Met Trp 130 135 140 Asn Ile Ser Ser Asp 145 <210> 135 <211> 1315 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1285) <223> RXA03503 <400> 135 tgtcacggaa tgcactttca cttacattga gtcccaaatc actgtgaggt gcaccattta 60 atggacttca cctccagtgg gtcaatgaaa ggagttcccc atg aac cac gta cca 115 Met Asn His Val Pro 1 5 gtg gca att att ggc gca gga cca gca gga cta acc ctc gcc cac ctc 163 Val Ala Ile Ile Gly Ala Gly Pro Ala Gly Leu Thr Leu Ala His Leu 10 15 20 ctc cac ctt caa ggt gtg gaa tca atc gtc ttt gaa tcc cgc acc cgc 211 Leu His Leu Gln Gly Val Glu Ser Ile Val Phe Glu Ser Arg Thr Arg 25 30 35 aag gac gtc gaa gaa acc gtc cga gca ggc atc ctg gaa caa ggc acc 259 Lys Asp Val Glu Glu Thr Val Arg Ala Gly Ile Leu Glu Gln Gly Thr 40 45 50 ctg aat ctg atg cgc gaa acc gga gtc ggc gca cgc atg gaa gca gaa 307 Leu Asn Leu Met Arg Glu Thr Gly Val Gly Ala Arg Met Glu Ala Glu 55 60 65 gcc gat cac gat gaa gca atc gac atc tcc atc aac aat gag cgc acc 355 Ala Asp His Asp Glu Ala Ile Asp Ile Ser Ile Asn Asn Glu Arg Thr 70 75 80 85 cgc att ccg ctg acc gaa ctc acc ggc cac aag gtt gcg atc tac ccg 403 Arg Ile Pro Leu Thr Glu Leu Thr Gly His Lys Val Ala Ile Tyr Pro 90 95 100 cag cac gaa tac ctc aaa gat ttc att gcc aag cgc atc gaa gat ggc 451 Gln His Glu Tyr Leu Lys Asp Phe Ile Ala Lys Arg Ile Glu Asp Gly 105 110 115 ggc gaa ctc ctt ttc acc acc act gtt gat tcc gta gaa aac tac gaa 499 Gly Glu Leu Leu Phe Thr Thr Thr Thr Val Asp Ser Val Glu Asn Tyr Glu 120 125 130 ggc gac ctc gcc aag gtg acc tac acc gaa gcc gat ggt tcc tcc acc 547 Gly Asp Leu Ala Lys Val Thr Tyr Thr Glu Ala Asp Gly Ser Ser Thr 135 140 145 acc atc acc gcc gac tac gtc atc gca gct gac ggc tcc aac tcc cct 595 Thr Ile Thr Ala Asp Tyr Val Ile Ala Ala Asp Gly Ser Asn Ser Pro 150 155 160 165 tac cgc aag ctg atc acc gaa gac ggt ggc gtg cgc gcc cgc cat gaa 643 Tyr Arg Lys Leu Ile Thr Glu Asp Gly Gly Val Arg Ala Arg His Glu 170 175 180 tac cct tac gca tgg ttc ggc att ttg gtg gaa gca cca aaa acc caa 691 Tyr Pro Tyr Ala Trp Phe Gly Ile Leu Val Glu Ala Pro Lys Thr Gln 185 190 195 aag gaa ctc atc tac gca acc cac cct gag ggc ttt gcg ctg atc tcc 739 Lys Glu Leu Ile Tyr Ala Thr His Pro Glu Gly Phe Ala Leu Ile Ser 200 205 210 acc cgt acc gat gaa atc cag cgc tac tac ctg cag tgc aac cct gac 787 Thr Arg Thr Asp Glu Ile Gln Arg Tyr Tyr Leu Gln Cys Asn Pro Asp 215 220 225 gac acc cca gac atg tgg ccc gat gac cgc att tgg gaa cag ctg cac 835 Asp Thr Pro Asp Met Trp Pro Asp Asp Arg Ile Trp Glu Gln Leu His 230 235 240 245 ctg cgt gcg gac tcc cct ggc atc acc gtg tct gaa ggg cgc atc ttt 883 Leu Arg Ala Asp Ser Pro Gly Ile Thr Val Ser Glu Gly Arg Ile Phe 250 255 260 gac aag gcc gtg ctg cgt ttc cgc tcc gcg gtc acc gaa cca atg caa 931 Asp Lys Ala Val Leu Arg Phe Arg Ser Ala Val Thr Glu Pro Met Gln 265 270 275 aag gga cgc ctc ttc ctt gct ggc gat gct gca cac acc gtg ccg cca 979 Lys Gly Arg Leu Phe Leu Ala Gly Asp Ala Ala His Thr Val Pro Pro 280 285 290 acc gga gct aag ggc ctc aac ttg gct gtt gcc gat gtc tca gta ctc 1027 Thr Gly Ala Lys Gly Leu Asn Leu Ala Val Ala Asp Val Ser Val Leu 295 300 305 gcg cca gca ctg gtt cgt gcc ctg aag aag aag gac acc ggc ttg ctc 1075 Ala Pro Ala Leu Val Arg Ala Leu Lys Lys Lys Asp Thr Gly Leu Leu 310 315 320 325 gat agc tac acc tcc ctg gca gtc ccc cgc atc tgg aaa gca cag cac 1123 Asp Ser Tyr Thr Ser Leu Ala Val Pro Arg Ile Trp Lys Ala Gln His 330 335 340 ttc tcc tac tgg atg agc tcc atg ctc cac gca gta ccc ggc gaa gat 1171 Phe Ser Tyr Trp Met Ser Ser Met Leu His Ala Val Pro Gly Glu Asp 345 350 355 cac ttt gcc acc cag cgc cga ttc gct gaa ttg cgc tcc gtc cta gaa 1219 His Phe Ala Thr Gln Arg Arg Phe Ala Glu Leu Arg Ser Val Leu Glu 360 365 370 tcc caa tcc ggc caa cgc tac ctc gca gag cag tac gtt ggg cgc gac 1267 Ser Gln Ser Gly Gln Arg Tyr Leu Ala Glu Gln Tyr Val Gly Arg Asp 375 380 385 cta cca cgc ttc gag gta taaacgctcg tgtcaacgac caccccaacc 1315 Leu Pro Arg Phe Glu Val 390 395 <210> 136 <211> 395 <212> PRT <213> Corynebacterium glutamicum <400> 136 Met Asn His Val Pro Val Ala Ile Ile Gly Ala Gly Pro Ala Gly Leu 1 5 10 15 Thr Leu Ala His Leu Leu His Leu Gln Gly Val Glu Ser Ile Val Phe 20 25 30 Glu Ser Arg Thr Arg Lys Asp Val Glu Glu Thr Val Arg Ala Gly Ile 35 40 45 Leu Glu Gln Gly Thr Leu Asn Leu Met Arg Glu Thr Gly Val Gly Ala 50 55 60 Arg Met Glu Ala Glu Ala Asp His Asp Glu Ala Ile Asp Ile Ser Ile 65 70 75 80 Asn Asn Glu Arg Thr Arg Ile Pro Leu Thr Glu Leu Thr Gly His Lys 85 90 95 Val Ala Ile Tyr Pro Gln His Glu Tyr Leu Lys Asp Phe Ile Ala Lys 100 105 110 Arg Ile Glu Asp Gly Gly Glu Leu Leu Phe Thr Thr Thr Val Val Asp Ser 115 120 125 Val Glu Asn Tyr Glu Gly Asp Leu Ala Lys Val Thr Tyr Thr Glu Ala 130 135 140 Asp Gly Ser Ser Thr Thr Ile Thr Ala Asp Tyr Val Ile Ala Ala Asp 145 150 155 160 Gly Ser Asn Ser Pro Tyr Arg Lys Leu Ile Thr Glu Asp Gly Gly Val 165 170 175 Arg Ala Arg His Glu Tyr Pro Tyr Ala Trp Phe Gly Ile Leu Val Glu 180 185 190 Ala Pro Lys Thr Gln Lys Glu Leu Ile Tyr Ala Thr His Pro Glu Gly 195 200 205 Phe Ala Leu Ile Ser Thr Arg Thr Asp Glu Ile Gln Arg Tyr Tyr Leu 210 215 220 Gln Cys Asn Pro Asp Asp Thr Pro Asp Met Trp Pro Asp Asp Arg Ile 225 230 235 240 Trp Glu Gln Leu His Leu Arg Ala Asp Ser Pro Gly Ile Thr Val Ser 245 250 255 Glu Gly Arg Ile Phe Asp Lys Ala Val Leu Arg Phe Arg Ser Ala Val 260 265 270 Thr Glu Pro Met Gln Lys Gly Arg Leu Phe Leu Ala Gly Asp Ala Ala 275 280 285 His Thr Val Pro Pro Thr Gly Ala Lys Gly Leu Asn Leu Ala Val Ala 290 295 300 Asp Val Ser Val Leu Ala Pro Ala Leu Val Arg Ala Leu Lys Lys Lys 305 310 315 320 Asp Thr Gly Leu Leu Asp Ser Tyr Thr Ser Leu Ala Val Pro Arg Ile 325 330 335 Trp Lys Ala Gln His Phe Ser Tyr Trp Met Ser Ser Met Leu His Ala 340 345 350 Val Pro Gly Glu Asp His Phe Ala Thr Gln Arg Arg Phe Ala Glu Leu 355 360 365 Arg Ser Val Leu Glu Ser Gln Ser Gly Gln Arg Tyr Leu Ala Glu Gln 370 375 380 Tyr Val Gly Arg Asp Leu Pro Arg Phe Glu Val 385 390 395 <210> 137 <211> 541 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (511) <223> RXA03545 <400> 137 agcttgtcga cgccacgacc cacaccttgc ttcaccacct cgggggcacc caccgacatt 60 aaagggaacc aaatagcgtt cgctgcagtc caaccaagat atg ggg att ttt cta 115 Met Gly Ile Phe Leu 1 5 gtt ggg ttg tgg tgc gca gtc ttg gct gtc atc gat ata aaa tac cgg 163 Val Gly Leu Trp Cys Ala Val Leu Ala Val Ile Asp Ile Lys Tyr Arg 10 15 20 cgc ctg ccc gat gtg ctc acc ctt cct ggt gca ctg tta att aat tta 211 Arg Leu Pro Asp Val Leu Thr Leu Pro Gly Ala Leu Leu Ile Asn Leu 25 30 35 tgg gca ttg act att tat cca cag tgg atg ctc ggc ggg ctt gtg tgg 259 Trp Ala Leu Thr Ile Tyr Pro Gln Trp Met Leu Gly Gly Leu Val Trp 40 45 50 gcg ggg att tat ttc ctc acg gca gtg ctg gtg gga gga att ggt ggg 307 Ala Gly Ile Tyr Phe Leu Thr Ala Val Leu Val Gly Gly Ile Gly Gly 55 60 65 gga gat atc aag ttg gct ctc ggt ttg ggg aca gcc gta gca gcc ttg 355 Gly Asp Ile Lys Leu Ala Leu Gly Leu Gly Thr Ala Val Ala Ala Leu 70 75 80 85 gga atg cag gct ctc atg gtg gca att gtg gga tca tcg tta att tct 403 Gly Met Gln Ala Leu Met Val Ala Ile Val Gly Ser Ser Leu Ile Ser 90 95 100 gtc tta ctg ggt gga att ttt gcg gct gtt aaa ggg gtg aaa agg ccc 451 Val Leu Leu Gly Gly Ile Phe Ala Ala Val Lys Gly Val Lys Arg Pro 105 110 115 aaa ttt att cca cat ggt cca agt atg gtt atc gct agt gcc tgc agt 499 Lys Phe Ile Pro His Gly Pro Ser Met Val Ile Ala Ser Ala Cys Ser 120 125 130 tat gtc gct ttt tgacgtcgaa aagcaatttt aaaaagccct 541 Tyr Val Ala Phe 135 <210> 138 <211> 137 <212> PRT <213> Corynebacterium glutamicum <400> 138 Met Gly Ile Phe Leu Val Gly Leu Trp Cys Ala Val Leu Ala Val Ile 1 5 10 15 Asp Ile Lys Tyr Arg Arg Leu Pro Asp Val Leu Thr Leu Pro Gly Ala 20 25 30 Leu Leu Ile Asn Leu Trp Ala Leu Thr Ile Tyr Pro Gln Trp Met Leu 35 40 45 Gly Gly Leu Val Trp Ala Gly Ile Tyr Phe Leu Thr Ala Val Leu Val 50 55 60 Gly Gly Ile Gly Gly Gly Asp Ile Lys Leu Ala Leu Gly Leu Gly Thr 65 70 75 80 Ala Val Ala Ala Leu Gly Met Gln Ala Leu Met Val Ala Ile Val Gly 85 90 95 Ser Ser Leu Ile Ser Val Leu Leu Gly Gly Ile Phe Ala Ala Val Lys 100 105 110 Gly Val Lys Arg Pro Lys Phe Ile Pro His Gly Pro Ser Met Val Ile 115 120 125 Ala Ser Ala Cys Ser Tyr Val Ala Phe 130 135 <139> <211> 1141 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1111) <223> RXA03669 <400> 139 gctgatcacc gtatggctac tgctggtgcg atcattggcc tcgcggttga tggcgttcag 60 gttgaagaca ttaagaccac ttccaaaact ttccctggtt ttg aaa atg ttt ggg 115 Leu Lys Met Phe Gly 1 5 agg aga tgg ttg gct aga cgc agc tat gac gaa tcc gat gtt cgt gtt 163 Arg Arg Trp Leu Ala Arg Arg Ser Tyr Asp Glu Ser Asp Val Arg Val 10 15 20 cgc cca ggt aaa ggt acc cgc cct cgt acg aag gat cgg cca tcg cat 211 Arg Pro Gly Lys Gly Thr Arg Pro Arg Thr Lys Asp Arg Pro Ser His 25 30 35 gag aat gct cta gtg ggc atg gtt gtg acg aag gat cgt ggt cgt tgg 259 Glu Asn Ala Leu Val Gly Met Val Val Thr Lys Asp Arg Gly Arg Trp 40 45 50 ggt gtt gtt ctc gat ggt cgt caa gat gcc att gtg acg atg cgt gcc 307 Gly Val Val Leu Asp Gly Arg Gln Asp Ala Ile Val Thr Met Arg Ala 55 60 65 cgt gag ttg ggg cgt acg gct atc gaa gtg ggt gat cgt gtc gga gtt 355 Arg Glu Leu Gly Arg Thr Ala Ile Glu Val Gly Asp Arg Val Gly Val 70 75 80 85 gtc ggc gat acg tct ggc cgt ccg ggc tcg ttg gct cga att gtc cgc 403 Val Gly Asp Thr Ser Gly Arg Pro Gly Ser Leu Ala Arg Ile Val Arg 90 95 100 ctt gag gag cgc acg agt gtg ttg cgt cgt acg gct gat gac acg gac 451 Leu Glu Glu Arg Thr Ser Val Leu Arg Arg Thr Ala Asp Asp Thr Asp 105 110 115 ccg ttt gag cgg att gtg gtg gct aat gcg gat cag ttg ttg att gtt 499 Pro Phe Glu Arg Ile Val Val Ala Asn Ala Asp Gln Leu Leu Ile Val 120 125 130 tct gct gtc gcc gat ccg ccg cct cgg gcg ggt ttt gtg gag cgt gct 547 Ser Ala Val Ala Asp Pro Pro Pro Arg Ala Gly Phe Val Glu Arg Ala 135 140 145 cta att gct gcg ttt gtt ggt aat ttg cag ccg gtg ttg tgt ttg acg 595 Leu Ile Ala Ala Phe Val Gly Asn Leu Gln Pro Val Leu Cys Leu Thr 150 155 160 165 aag tct gat ttg gcg gat cct gcg gag ttc gct gcg gag ttt gag gct 643 Lys Ser Asp Leu Ala Asp Pro Ala Glu Phe Ala Ala Glu Phe Glu Ala 170 175 180 ttg gaa gtg ccg gtt gtt gtt tgt ggt gtt gat gat cct ctt gat cct 691 Leu Glu Val Pro Val Val Val Cys Gly Val Asp Asp Pro Leu Asp Pro 185 190 195 gtt ttg gag gtc gtg gag ggc cat att acg gcg ctt att ggg caa tct 739 Val Leu Glu Val Val Glu Gly His Ile Thr Ala Leu Ile Gly Gln Ser 200 205 210 ggt gtg ggt aag tcg acg ttg gtg aat cgt ttg gtg ccg gat gcc gat 787 Gly Val Gly Lys Ser Thr Leu Val Asn Arg Leu Val Pro Asp Ala Asp 215 220 225 cgt gag act ggt gtc gtg tcg ggg gtt ggc aag ggt cgg cat acg tcg 835 Arg Glu Thr Gly Val Val Ser Gly Val Gly Lys Gly Arg His Thr Ser 230 235 240 245 acg cag tcg gtc gct ctt gcc att gat aat ggt tgg att atc gaa acg 883 Thr Gln Ser Val Ala Leu Ala Ile Asp Asn Gly Trp Ile Ile Glu Thr 250 255 260 ccg ggt att cgt tcg ttt ggt ttg gcg cat gtt gat gcg gac act gtg 931 Pro Gly Ile Arg Ser Phe Gly Leu Ala His Val Asp Ala Asp Thr Val 265 270 275 gtt ggt gtg ttc gac gat ttg gct tta gcg gcg gag gat tgt cct cgt 979 Val Gly Val Phe Asp Asp Leu Ala Leu Ala Ala Glu Asp Cys Pro Arg 280 285 290 ggg tgt tcg cat ttg ggt cct ccg gcg gat cct ggt tgt gcg ttg gat 1027 Gly Cys Ser His Leu Gly Pro Pro Ala Asp Pro Gly Cys Ala Leu Asp 295 300 305 cgc ctg aag ggt gcg agt gcg cgt cgt gtt gag gcg gtg cgt gcg ttg 1075 Arg Leu Lys Gly Ala Ser Ala Arg Arg Val Glu Ala Val Arg Ala Leu 310 315 320 325 ctc gtt gcg ttg agg agc aac gac gcg tgg gat atg tgatctagag 1121 Leu Val Ala Leu Arg Ser Asn Asp Ala Trp Asp Met 330 335 gagctcgact aggaagggga 1141 <210> 140 <211> 337 <212> PRT <213> Corynebacterium glutamicum <400> 140 Leu Lys Met Phe Gly Arg Arg Trp Leu Ala Arg Arg Ser Tyr Asp Glu 1 5 10 15 Ser Asp Val Arg Val Arg Pro Gly Lys Gly Thr Arg Pro Arg Thr Lys 20 25 30 Asp Arg Pro Ser His Glu Asn Ala Leu Val Gly Met Val Val Thr Lys 35 40 45 Asp Arg Gly Arg Trp Gly Val Val Leu Asp Gly Arg Gln Asp Ala Ile 50 55 60 Val Thr Met Arg Ala Arg Glu Leu Gly Arg Thr Ala Ile Glu Val Gly 65 70 75 80 Asp Arg Val Gly Val Val Gly Asp Thr Ser Gly Arg Pro Gly Ser Leu 85 90 95 Ala Arg Ile Val Arg Leu Glu Glu Arg Thr Ser Val Leu Arg Arg Thr 100 105 110 Ala Asp Asp Thr Asp Pro Phe Glu Arg Ile Val Val Ala Asn Ala Asp 115 120 125 Gln Leu Leu Ile Val Ser Ala Val Ala Asp Pro Pro Pro Arg Ala Gly 130 135 140 Phe Val Glu Arg Ala Leu Ile Ala Ala Phe Val Gly Asn Leu Gln Pro 145 150 155 160 Val Leu Cys Leu Thr Lys Ser Asp Leu Ala Asp Pro Ala Glu Phe Ala 165 170 175 Ala Glu Phe Glu Ala Leu Glu Val Pro Val Val Val Cys Gly Val Asp 180 185 190 Asp Pro Leu Asp Pro Val Leu Glu Val Val Glu Gly His Ile Thr Ala 195 200 205 Leu Ile Gly Gln Ser Gly Val Gly Lys Ser Thr Leu Val Asn Arg Leu 210 215 220 Val Pro Asp Ala Asp Arg Glu Thr Gly Val Val Ser Gly Val Gly Lys 225 230 235 240 Gly Arg His Thr Ser Thr Gln Ser Val Ala Leu Ala Ile Asp Asn Gly 245 250 255 Trp Ile Ile Glu Thr Pro Gly Ile Arg Ser Phe Gly Leu Ala His Val 260 265 270 Asp Ala Asp Thr Val Val Gly Val Phe Asp Asp Leu Ala Leu Ala Ala 275 280 285 Glu Asp Cys Pro Arg Gly Cys Ser His Leu Gly Pro Pro Ala Asp Pro 290 295 300 Gly Cys Ala Leu Asp Arg Leu Lys Gly Ala Ser Ala Arg Arg Val Glu 305 310 315 320 Ala Val Arg Ala Leu Leu Val Ala Leu Arg Ser Asn Asp Ala Trp Asp 325 330 335 Met <210> 141 <211> 751 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (721) <223> RXA03993 <400> 141 ggtgagatcg tcggcaaggc tttgcaggct gtgcgcgctg aggctaaagc tggcatgagc 60 acgtgggatc tggatcagat cgcggagcag gttatccgcg atg ctg gcg ccg ttc 115 Met leu ala pro phe 1 5 cta cat tcc tgg gtt acc agg gtt ttc ccg gca tca gtg tgc gct tcg 163 Leu His Ser Trp Val Thr Arg Val Phe Pro Ala Ser Val Cys Ala Ser 10 15 20 gtc aat gag gtg att gtt cac ggc att cca tcc aag gag acc atc ttg 211 Val Asn Glu Val Ile Val His Gly Ile Pro Ser Lys Glu Thr Ile Leu 25 30 35 gag gaa ggc gat ctg gtg tcc atc gac tgc ggc gca acc ttt gat ggt 259 Glu Glu Gly Asp Leu Val Ser Ile Asp Cys Gly Ala Thr Phe Asp Gly 40 45 50 tgg gtc ggc gat tcc gcg tgg agc ttc ggc atc ggc gag ctg gac gag 307 Trp Val Gly Asp Ser Ala Trp Ser Phe Gly Ile Gly Glu Leu Asp Glu 55 60 65 gac gtc cag ggt ctc aac ttg gct acc gag tgg gtc ctc atg gaa ggc 355 Asp Val Gln Gly Leu Asn Leu Ala Thr Glu Trp Val Leu Met Glu Gly 70 75 80 85 atg aag gcc atg gtt cca ggc aac cgt ttg acc gat gtc tcc cac gct 403 Met Lys Ala Met Val Pro Gly Asn Arg Leu Thr Asp Val Ser His Ala 90 95 100 ctc gag gtc gca acc cgc aag gct gag tcc aag ttc ggc gtc gcg ctc 451 Leu Glu Val Ala Thr Arg Lys Ala Glu Ser Lys Phe Gly Val Ala Leu 105 110 115 ggc atc gtc gat ggc tac ggc gga cac ggc att ggc cgc cac atg cac 499 Gly Ile Val Asp Gly Tyr Gly Gly His Gly Ile Gly Arg His Met His 120 125 130 gag gag cca tac ttg gct aat gag ggc aag gcc ggc aag ggc cct gtg 547 Glu Glu Pro Tyr Leu Ala Asn Glu Gly Lys Ala Gly Lys Gly Pro Val 135 140 145 att cag gag ggc tcc gtg ctc gcc att gag cct atg ctc acc ctc ggc 595 Ile Gln Glu Gly Ser Val Leu Ala Ile Glu Pro Met Leu Thr Leu Gly 150 155 160 165 acc gaa gat tcc gca gtg ctg gaa gat gat tgg act gtc gtg act ctc 643 Thr Glu Asp Ser Ala Val Leu Glu Asp Asp Trp Thr Val Val Thr Leu 170 175 180 gac ggt tca tgg gca tca cac tgg gag cac acc gtt gca gcc acc aag 691 Asp Gly Ser Trp Ala Ser His Trp Glu His Thr Val Ala Ala Thr Lys 185 190 195 ggc ggc ccg cgc atc ctc acg ccg cgt tat taaaatgatg cttttcgacg 741 Gly Gly Pro Arg Ile Leu Thr Pro Arg Tyr 200 205 catccccacg 751 <210> 142 <211> 207 <212> PRT <213> Corynebacterium glutamicum <400> 142 Met Leu Ala Pro Phe Leu His Ser Trp Val Thr Arg Val Phe Pro Ala 1 5 10 15 Ser Val Cys Ala Ser Val Asn Glu Val Ile Val His Gly Ile Pro Ser 20 25 30 Lys Glu Thr Ile Leu Glu Glu Gly Asp Leu Val Ser Ile Asp Cys Gly 35 40 45 Ala Thr Phe Asp Gly Trp Val Gly Asp Ser Ala Trp Ser Phe Gly Ile 50 55 60 Gly Glu Leu Asp Glu Asp Val Gln Gly Leu Asn Leu Ala Thr Glu Trp 65 70 75 80 Val Leu Met Glu Gly Met Lys Ala Met Val Pro Gly Asn Arg Leu Thr 85 90 95 Asp Val Ser His Ala Leu Glu Val Ala Thr Arg Lys Ala Glu Ser Lys 100 105 110 Phe Gly Val Ala Leu Gly Ile Val Asp Gly Tyr Gly Gly His Gly Ile 115 120 125 Gly Arg His Met His Glu Glu Pro Tyr Leu Ala Asn Glu Gly Lys Ala 130 135 140 Gly Lys Gly Pro Val Ile Gln Glu Gly Ser Val Leu Ala Ile Glu Pro 145 150 155 160 Met Leu Thr Leu Gly Thr Glu Asp Ser Ala Val Leu Glu Asp Asp Trp 165 170 175 Thr Val Val Thr Leu Asp Gly Ser Trp Ala Ser His Trp Glu His Thr 180 185 190 Val Ala Ala Thr Lys Gly Gly Pro Arg Ile Leu Thr Pro Arg Tyr 195 200 205 <210> 143 <211> 1537 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (1507) <223> RXA04039 <400> 143 atgaggctga gaagcgattc aagtcgatag atccggggga tcttctgcgc tagaaacttg 60 tcccgagggg ctgggttggt gtgcacactg ctgtggcttt gtg tgt gca caa cct 115 Val Cys Ala Gln Pro 1 5 agt ggg ata ggg cgt gca caa gga tat gac att ggg tgt gca caa cgt 163 Ser Gly Ile Gly Arg Ala Gln Gly Tyr Asp Ile Gly Cys Ala Gln Arg 10 15 20 gct gac act tta cag act ggt ttt caa cta atg aca ccg aaa gaa ata 211 Ala Asp Thr Leu Gln Thr Gly Phe Gln Leu Met Thr Pro Lys Glu Ile 25 30 35 cac ctc aac ctt ttt gct ttc ggt gcc ggg cac cac gc gcg gcg gcg tgg 259 His Leu Asn Leu Phe Ala Phe Gly Ala Gly His His Ala Ala Ala Trp 40 45 50 cga gcg gtg gag gga agc gtc gaa aag ctg ggt tta att tcc tgg tgg 307 Arg Ala Val Glu Gly Ser Val Glu Lys Leu Gly Leu Ile Ser Trp Trp 55 60 65 gag gaa ctc gcg cgc acc gct gag cgg ggc aag ctg gat gcg gtc ttt 355 Glu Glu Leu Ala Arg Thr Ala Glu Arg Gly Lys Leu Asp Ala Val Phe 70 75 80 85 ttg gcc gat ggg cag gcg att aat ccg gtc ggt ctg gag aat ggg ccg 403 Leu Ala Asp Gly Gln Ala Ile Asn Pro Val Gly Leu Glu Asn Gly Pro 90 95 100 ggc tgg ttt ttg gag ccg gtg acc gcg ttg act gcg atg gcg cgg gcg 451 Gly Trp Phe Leu Glu Pro Val Thr Ala Leu Thr Ala Met Ala Arg Ala 105 110 115 acg aac aat att ggg ttg atc agc aca att tcc agt acg ttt tgg cag 499 Thr Asn Asn Ile Gly Leu Ile Ser Thr Ile Ser Ser Thr Phe Trp Gln 120 125 130 ccg ttt cat gcg gcg cgg atg atc gcc agc ttg gat cat att tcg ggt 547 Pro Phe His Ala Ala Arg Met Ile Ala Ser Leu Asp His Ile Ser Gly 135 140 145 ggg cgt gct gga atc aat gtg gtg aca tcg atg acc gat gcg gag gcg 595 Gly Arg Ala Gly Ile Asn Val Val Thr Ser Met Thr Asp Ala Glu Ala 150 155 160 165 cgt aac cac ggg atg gat gcg ttg ccg ggt cac gat gtt cgc tat gcg 643 Arg Asn His Gly Met Asp Ala Leu Pro Gly His Asp Val Arg Tyr Ala 170 175 180 cgc gct gcg gaa ttt att gaa acc atc act gcg ctg tgg gat tct tgg 691 Arg Ala Ala Glu Phe Ile Glu Thr Ile Thr Ala Leu Trp Asp Ser Trp 185 190 195 cct gcg gaa agt ttg gtg atg gat cgt gct gga aaa ttt gcg gac tcc 739 Pro Ala Glu Ser Leu Val Met Asp Arg Ala Gly Lys Phe Ala Asp Ser 200 205 210 tcg ctc att aaa tct atc gat cat gat ggt gag ttc ttc caa gtc gct 787 Ser Leu Ile Lys Ser Ile Asp His Asp Gly Glu Phe Phe Gln Val Ala 215 220 225 ggt ccg ctg aat atc ccc agt cct ccg cag ggt cga ccc gta ctt ttt 835 Gly Pro Leu Asn Ile Pro Ser Pro Pro Gln Gly Arg Pro Val Leu Phe 230 235 240 245 cag gct gga tcc tca ccg caa gga cgg gaa atc gct gcg aaa tac gcc 883 Gln Ala Gly Ser Ser Pro Gln Gly Arg Glu Ile Ala Ala Lys Tyr Ala 250 255 260 gag gca att tac tct gtg gcg tgg gat ttg gag caa gcg caa gat tat 931 Glu Ala Ile Tyr Ser Val Ala Trp Asp Leu Glu Gln Ala Gln Asp Tyr 265 270 275 cgc tct gat att cat gct cgt gcc act gcc cag ggt cgc gag ccc atg 979 Arg Ser Asp Ile His Ala Arg Ala Thr Ala Gln Gly Arg Glu Pro Met 280 285 290 ccg gtg ctt cct ggt ttg gtg act ttt gtt ggc acg acc gtg gaa gaa 1027 Pro Val Leu Pro Gly Leu Val Thr Phe Val Gly Thr Thr Val Glu Glu 295 300 305 gcg cgt gca aaa cag cag gct ctt aat gcg ttg ctg ccg gtc aaa gac 1075 Ala Arg Ala Lys Gln Gln Ala Leu Asn Ala Leu Leu Pro Val Lys Asp 310 315 320 325 tca cta aat cag ttg agt ttc ttt gtg ggt caa gat tgc tcg acg tgg 1123 Ser Leu Asn Gln Leu Ser Phe Phe Val Gly Gln Asp Cys Ser Thr Trp 330 335 340 gat ttg gat gca cct ccc cca cca ctg cca ccg cta gaa gag ttt tcc 1171 Asp Leu Asp Ala Pro Pro Pro Pro Leu Pro Pro Leu Glu Glu Phe Ser 345 350 355 ggt cct aaa ggc agg tac gaa acg gtc ctg cgg atc atc gaa tct aaa 1219 Gly Pro Lys Gly Arg Tyr Glu Thr Val Leu Arg Ile Ile Glu Ser Lys 360 365 370 tca cca acc gtc cgc gaa ctt ttg ggc tac ctc gca gca ggc ggt ggg 1267 Ser Pro Thr Val Arg Glu Leu Leu Gly Tyr Leu Ala Ala Gly Gly Gly 375 380 385 cac gcc aca ttt atc ggc act ccg gaa agc atc gct gat gaa atc gag 1315 His Ala Thr Phe Ile Gly Thr Pro Glu Ser Ile Ala Asp Glu Ile Glu 390 395 400 405 cgc tgg atc gac gag ggc ggt gcc gat ggc ttc aac ctc atg cca ccg 1363 Arg Trp Ile Asp Glu Gly Gly Ala Asp Gly Phe Asn Leu Met Pro Pro 410 415 420 atg ctc ccc cac agt ttg gac gac ttt gtt gac tta gta gtc cct gaa 1411 Met Leu Pro His Ser Leu Asp Asp Phe Val Asp Leu Val Val Pro Glu 425 430 435 ctg cag cga cgt ggc cga ttc cgc aca gaa tac agt cac acc acg ctg 1459 Leu Gln Arg Arg Gly Arg Phe Arg Thr Glu Tyr Ser His Thr Thr Leu 440 445 450 cgc ggt cac ttg ttt aac cag gtt gct tat tca gcc cgg tca ctt gtt 1507 Arg Gly His Leu Phe Asn Gln Val Ala Tyr Ser Ala Arg Ser Leu Val 455 460 465 taactaggtc acttgtttaa ctcagcaatt 1537 <210> 144 <211> 469 <212> PRT <213> Corynebacterium glutamicum <400> 144 Val Cys Ala Gln Pro Ser Gly Ile Gly Arg Ala Gln Gly Tyr Asp Ile 1 5 10 15 Gly Cys Ala Gln Arg Ala Asp Thr Leu Gln Thr Gly Phe Gln Leu Met 20 25 30 Thr Pro Lys Glu Ile His Leu Asn Leu Phe Ala Phe Gly Ala Gly His 35 40 45 His Ala Ala Ala Trp Arg Ala Val Glu Gly Ser Val Glu Lys Leu Gly 50 55 60 Leu Ile Ser Trp Trp Glu Glu Leu Ala Arg Thr Ala Glu Arg Gly Lys 65 70 75 80 Leu Asp Ala Val Phe Leu Ala Asp Gly Gln Ala Ile Asn Pro Val Gly 85 90 95 Leu Glu Asn Gly Pro Gly Trp Phe Leu Glu Pro Val Thr Ala Leu Thr 100 105 110 Ala Met Ala Arg Ala Thr Asn Asn Ile Gly Leu Ile Ser Thr Ile Ser 115 120 125 Ser Thr Phe Trp Gln Pro Phe His Ala Ala Arg Met Ile Ala Ser Leu 130 135 140 Asp His Ile Ser Gly Gly Arg Ala Gly Ile Asn Val Val Thr Ser Met 145 150 155 160 Thr Asp Ala Glu Ala Arg Asn His Gly Met Asp Ala Leu Pro Gly His 165 170 175 Asp Val Arg Tyr Ala Arg Ala Ala Glu Phe Ile Glu Thr Ile Thr Ala 180 185 190 Leu Trp Asp Ser Trp Pro Ala Glu Ser Leu Val Met Asp Arg Ala Gly 195 200 205 Lys Phe Ala Asp Ser Ser Leu Ile Lys Ser Ile Asp His Asp Gly Glu 210 215 220 Phe Phe Gln Val Ala Gly Pro Leu Asn Ile Pro Ser Pro Pro Gln Gly 225 230 235 240 Arg Pro Val Leu Phe Gln Ala Gly Ser Ser Pro Gln Gly Arg Glu Ile 245 250 255 Ala Ala Lys Tyr Ala Glu Ala Ile Tyr Ser Val Ala Trp Asp Leu Glu 260 265 270 Gln Ala Gln Asp Tyr Arg Ser Asp Ile His Ala Arg Ala Thr Ala Gln 275 280 285 Gly Arg Glu Pro Met Pro Val Leu Pro Gly Leu Val Thr Phe Val Gly 290 295 300 Thr Thr Val Glu Glu Ala Arg Ala Lys Gln Gln Ala Leu Asn Ala Leu 305 310 315 320 Leu Pro Val Lys Asp Ser Leu Asn Gln Leu Ser Phe Phe Val Gly Gln 325 330 335 Asp Cys Ser Thr Trp Asp Leu Asp Ala Pro Pro Pro Leu Pro Pro 340 345 350 Leu Glu Glu Phe Ser Gly Pro Lys Gly Arg Tyr Glu Thr Val Leu Arg 355 360 365 Ile Ile Glu Ser Lys Ser Pro Thr Val Arg Glu Leu Leu Gly Tyr Leu 370 375 380 Ala Ala Gly Gly Gly His Ala Thr Phe Ile Gly Thr Pro Glu Ser Ile 385 390 395 400 Ala Asp Glu Ile Glu Arg Trp Ile Asp Glu Gly Gly Ala Asp Gly Phe 405 410 415 Asn Leu Met Pro Pro Met Leu Pro His Ser Leu Asp Asp Phe Val Asp 420 425 430 Leu Val Val Pro Glu Leu Gln Arg Arg Gly Arg Phe Arg Thr Glu Tyr 435 440 445 Ser His Thr Thr Leu Arg Gly His Leu Phe Asn Gln Val Ala Tyr Ser 450 455 460 Ala Arg Ser Leu Val 465 <210> 145 <211> 2248 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (2218) <223> RXA04049 <400> 145 aaatgaatcc gggtttttca gtttcggggt gcaaatcaga atgtcgccaa tggcgaacac 60 acgagcgtgc agaagatgtg cgtgactaag atcgggggct atg tct gaa cgc cta 115 Met ser glu arg leu 1 5 aac gct ccg caa gca cca atc cat ccc atc acc cga acc cac cac ggt 163 Asn Ala Pro Gln Ala Pro Ile His Pro Ile Thr Arg Thr His His Gly 10 15 20 att gat ttc gta gac aac tat gaa tgg ctg agg gat aaa gaa tcc caa 211 Ile Asp Phe Val Asp Asn Tyr Glu Trp Leu Arg Asp Lys Glu Ser Gln 25 30 35 gaa acc ttg gac tac ctg gag gcg gag aat gcg ttc acc aag cag gag 259 Glu Thr Leu Asp Tyr Leu Glu Ala Glu Asn Ala Phe Thr Lys Gln Glu 40 45 50 act gaa cag cta gcc aca ctg cgg gac aac atc tat gaa gag att aag 307 Thr Glu Gln Leu Ala Thr Leu Arg Asp Asn Ile Tyr Glu Glu Ile Lys 55 60 65 tca cgc gtt aaa gaa acc gac atg tcc atc cca gtg cgt gcc gga aag 355 Ser Arg Val Lys Glu Thr Asp Met Ser Ile Pro Val Arg Ala Gly Lys 70 75 80 85 cac tgg tat tac tct cgc act gaa gaa ggc aag agc tac ggc tat tcc 403 His Trp Tyr Tyr Ser Arg Thr Glu Glu Gly Lys Ser Tyr Gly Tyr Ser 90 95 100 tgc cgc att cca gtg act gaa ggg tcg gat gca tgg acc cct cct gtt 451 Cys Arg Ile Pro Val Thr Glu Gly Ser Asp Ala Trp Thr Pro Pro Val 105 110 115 atc cct gag ggt gag cca gcg cag ggt gaa acc atc atc atg gat gcc 499 Ile Pro Glu Gly Glu Pro Ala Gln Gly Glu Thr Ile Ile Met Asp Ala 120 125 130 aac gag ttg gca gaa ggc cac gaa ttc ttc tcc atg ggt gca tca tct 547 Asn Glu Leu Ala Glu Gly His Glu Phe Phe Ser Met Gly Ala Ser Ser 135 140 145 gtc acc acc tct ggc cgc tac ctt gcg tat tcc acc gat gtc acg ggc 595 Val Thr Thr Ser Gly Arg Tyr Leu Ala Tyr Ser Thr Asp Val Thr Gly 150 155 160 165 gaa gag cgc ttt acg ttg cgc atc aag gat cta gaa act ggc gag ctg 643 Glu Glu Arg Phe Thr Leu Arg Ile Lys Asp Leu Glu Thr Gly Glu Leu 170 175 180 ctt cct gat acc ctg act ggc att ttc tac ggt gct act tgg gtg ggg 691 Leu Pro Asp Thr Leu Thr Gly Ile Phe Tyr Gly Ala Thr Trp Val Gly 185 190 195 gag gag tac ctc ttt tac cag cgc gtt gat gat gcg tgg cgt cca gat 739 Glu Glu Tyr Leu Phe Tyr Gln Arg Val Asp Asp Ala Trp Arg Pro Asp 200 205 210 act gtg tgg cgc cac aag gtg ggt acc ccg gtt gaa gaa gac gtg ttg 787 Thr Val Trp Arg His Lys Val Gly Thr Pro Val Glu Glu Asp Val Leu 215 220 225 gtg tac cac gag cct gat gaa cgt tat tcc acc tgg gtg ggc acc act 835 Val Tyr His Glu Pro Asp Glu Arg Tyr Ser Thr Trp Val Gly Thr Thr 230 235 240 245 cgt tca gaa aag ttc atc ctt ttt ggt tgc gcc tcc aag atc acc tct 883 Arg Ser Glu Lys Phe Ile Leu Phe Gly Cys Ala Ser Lys Ile Thr Ser 250 255 260 gaa gtc cgc gtg ctt cct ttc gac cag cca gag ggc acc cct gag gtg 931 Glu Val Arg Val Leu Pro Phe Asp Gln Pro Glu Gly Thr Pro Glu Val 265 270 275 ctg att ccg cgc gcg gag ggt gtg gaa tac gac gtc gat cat gca gtc 979 Leu Ile Pro Arg Ala Glu Gly Val Glu Tyr Asp Val Asp His Ala Val 280 285 290 gta gac ggc tcc gat att tgg ttg gtc aca cac aac gcc gag ggc ccg 1027 Val Asp Gly Ser Asp Ile Trp Leu Val Thr His Asn Ala Glu Gly Pro 295 300 305 aac ttt tcg gtg ggg tgg gct ggc gtc gac aag ctc aat tct ttg gac 1075 Asn Phe Ser Val Gly Trp Ala Gly Val Asp Lys Leu Asn Ser Leu Asp 310 315 320 325 gcg ctg gcg cca ctc gtc gcg cac aag gat gac gtg cgc att gag ggt 1123 Ala Leu Ala Pro Leu Val Ala His Lys Asp Asp Val Arg Ile Glu Gly 330 335 340 gtc gat acc tac cgc gat ttc atc atc ctg ggc tac agg tcc ggc gcg 1171 Val Asp Thr Tyr Arg Asp Phe Ile Ile Leu Gly Tyr Arg Ser Gly Ala 345 350 355 atc ggc cag gtc gcg atc atg aag ctt atc gac gga acc ttc ggc gat 1219 Ile Gly Gln Val Ala Ile Met Lys Leu Ile Asp Gly Thr Phe Gly Asp 360 365 370 ttc caa cag ctg gaa ttt gac gag gaa atc tac acc gtc gca tcg ggc 1267 Phe Gln Gln Leu Glu Phe Asp Glu Glu Ile Tyr Thr Val Ala Ser Gly 375 380 385 gga aac cca gaa tgg gac gcc ccc gtc att cgc ctt tct tac gga tca 1315 Gly Asn Pro Glu Trp Asp Ala Pro Val Ile Arg Leu Ser Tyr Gly Ser 390 395 400 405 ttc acc acc ccg gcg cag ctg ttt aac tac tgg att gaa tcc ggc gaa 1363 Phe Thr Thr Pro Ala Gln Leu Phe Asn Tyr Trp Ile Glu Ser Gly Glu 410 415 420 cgc acg ctg ctg aag cag cag gaa gtg ctc ggc gga tac aag ccg tca 1411 Arg Thr Leu Leu Lys Gln Gln Glu Val Leu Gly Gly Tyr Lys Pro Ser 425 430 435 gac tat gtg gcc tcc cga ttg tgg gtc act gcg aaa gat ggc gcg cag 1459 Asp Tyr Val Ala Ser Arg Leu Trp Val Thr Ala Lys Asp Gly Ala Gln 440 445 450 att cca gtg tcc ttg gtg cac cgc acc gac ctg gat gta tcc aag ccc 1507 Ile Pro Val Ser Leu Val His Arg Thr Asp Leu Asp Val Ser Lys Pro 455 460 465 aac ccc acg ttg ctc tac ggc tat ggt tcc tac gaa tca tcc att gat 1555 Asn Pro Thr Leu Leu Tyr Gly Tyr Gly Ser Tyr Glu Ser Ser Ile Asp 470 475 480 485 cca ggc ttc tct atc gcg cgt ttg tca ctg atg gat cgt ggc atg att 1603 Pro Gly Phe Ser Ile Ala Arg Leu Ser Leu Met Asp Arg Gly Met Ile 490 495 500 ttt gcg att gcc cac gtt cgt ggc ggt ggc gaa atg ggt cgt ggc tgg 1651 Phe Ala Ile Ala His Val Arg Gly Gly Gly Glu Met Gly Arg Gly Trp 505 510 515 tac gac aac ggc aaa acc acc acg aag aaa aac acc ttc acc gac ttc 1699 Tyr Asp Asn Gly Lys Thr Thr Thr Lys Lys Asn Thr Phe Thr Asp Phe 520 525 530 att gat gtt gcc gac gcc ctc atc gag cag aag att tct gcc cct gaa 1747 Ile Asp Val Ala Asp Ala Leu Ile Glu Gln Lys Ile Ser Ala Pro Glu 535 540 545 atg ctg gtt gca gaa ggc ggc tca gct ggt ggc atg ctc atg ggc gcc 1795 Met Leu Val Ala Glu Gly Gly Ser Ala Gly Gly Met Leu Met Gly Ala 550 555 560 565 att gcc aac atg gcc ggt gac cgc ttc aag gcg atc gaa gcc aac gtg 1843 Ile Ala Asn Met Ala Gly Asp Arg Phe Lys Ala Ile Glu Ala Asn Val 570 575 580 cca ttc gtc gat ccg ctg acc tct atg ctc atg ccg gaa ctg cca ctg 1891 Pro Phe Val Asp Pro Leu Thr Ser Met Leu Met Pro Glu Leu Pro Leu 585 590 595 acg gtt atc gaa tgg gat gag tgg ggc gat cca ctc cac gat aag gac 1939 Thr Val Ile Glu Trp Asp Glu Trp Gly Asp Pro Leu His Asp Lys Asp 600 605 610 gtc tat gaa tac atg gcg tcg tat gcc cca tat gaa aac atc gag gca 1987 Val Tyr Glu Tyr Met Ala Ser Tyr Ala Pro Tyr Glu Asn Ile Glu Ala 615 620 625 aag aac tac ccc aat atc ttg gcc gta aca tcg ctc aac gac acc cga 2035 Lys Asn Tyr Pro Asn Ile Leu Ala Val Thr Ser Ser Leu Asn Asp Thr Arg 630 635 640 645 gtg ttg tac gtc gaa cca gcc aaa tgg gta gcg cag ctt cgg gcg act 2083 Val Leu Tyr Val Glu Pro Ala Lys Trp Val Ala Gln Leu Arg Ala Thr 650 655 660 gca acc ggt gga gaa ttc ctt ctg aaa act gaa atg gtt gcc gga cac 2131 Ala Thr Gly Gly Glu Phe Leu Leu Lys Thr Glu Met Val Ala Gly His 665 670 675 ggc ggt gtg tca gga cgc tac gaa aag tgg cgt gag act gca ttt gag 2179 Gly Gly Val Ser Gly Arg Tyr Glu Lys Trp Arg Glu Thr Ala Phe Glu 680 685 690 tac ggc tgg ttg atc aac caa gca acc ggt gtg acc gaa taaaacttgt 2228 Tyr Gly Trp Leu Ile Asn Gln Ala Thr Gly Val Thr Glu 695 700 705 tcgactagcg aactttaaat 2248 <210> 146 <211> 706 <212> PRT <213> Corynebacterium glutamicum <400> 146 Met Ser Glu Arg Leu Asn Ala Pro Gln Ala Pro Ile His Pro Ile Thr 1 5 10 15 Arg Thr His His Gly Ile Asp Phe Val Asp Asn Tyr Glu Trp Leu Arg 20 25 30 Asp Lys Glu Ser Gln Glu Thr Leu Asp Tyr Leu Glu Ala Glu Asn Ala 35 40 45 Phe Thr Lys Gln Glu Thr Glu Gln Leu Ala Thr Leu Arg Asp Asn Ile 50 55 60 Tyr Glu Glu Ile Lys Ser Arg Val Lys Glu Thr Asp Met Ser Ile Pro 65 70 75 80 Val Arg Ala Gly Lys His Trp Tyr Tyr Ser Arg Thr Glu Glu Gly Lys 85 90 95 Ser Tyr Gly Tyr Ser Cys Arg Ile Pro Val Thr Glu Gly Ser Asp Ala 100 105 110 Trp Thr Pro Pro Val Ile Pro Glu Gly Glu Pro Ala Gln Gly Glu Thr 115 120 125 Ile Ile Met Asp Ala Asn Glu Leu Ala Glu Gly His Glu Phe Phe Ser 130 135 140 Met Gly Ala Ser Ser Val Thr Thr Ser Gly Arg Tyr Leu Ala Tyr Ser 145 150 155 160 Thr Asp Val Thr Gly Glu Glu Arg Phe Thr Leu Arg Ile Lys Asp Leu 165 170 175 Glu Thr Gly Glu Leu Leu Pro Asp Thr Leu Thr Gly Ile Phe Tyr Gly 180 185 190 Ala Thr Trp Val Gly Glu Glu Tyr Leu Phe Tyr Gln Arg Val Asp Asp 195 200 205 Ala Trp Arg Pro Asp Thr Val Trp Arg His Lys Val Gly Thr Pro Val 210 215 220 Glu Glu Asp Val Leu Val Tyr His Glu Pro Asp Glu Arg Tyr Ser Thr 225 230 235 240 Trp Val Gly Thr Thr Arg Ser Glu Lys Phe Ile Leu Phe Gly Cys Ala 245 250 255 Ser Lys Ile Thr Ser Glu Val Arg Val Leu Pro Phe Asp Gln Pro Glu 260 265 270 Gly Thr Pro Glu Val Leu Ile Pro Arg Ala Glu Gly Val Glu Tyr Asp 275 280 285 Val Asp His Ala Val Val Asp Gly Ser Asp Ile Trp Leu Val Thr His 290 295 300 Asn Ala Glu Gly Pro Asn Phe Ser Val Gly Trp Ala Gly Val Asp Lys 305 310 315 320 Leu Asn Ser Leu Asp Ala Leu Ala Pro Leu Val Ala His Lys Asp Asp 325 330 335 Val Arg Ile Glu Gly Val Asp Thr Tyr Arg Asp Phe Ile Ile Leu Gly 340 345 350 Tyr Arg Ser Gly Ala Ile Gly Gln Val Ala Ile Met Lys Leu Ile Asp 355 360 365 Gly Thr Phe Gly Asp Phe Gln Gln Leu Glu Phe Asp Glu Glu Ile Tyr 370 375 380 Thr Val Ala Ser Gly Gly Asn Pro Glu Trp Asp Ala Pro Val Ile Arg 385 390 395 400 Leu Ser Tyr Gly Ser Phe Thr Thr Pro Ala Gln Leu Phe Asn Tyr Trp 405 410 415 Ile Glu Ser Gly Glu Arg Thr Leu Leu Lys Gln Gln Glu Val Leu Gly 420 425 430 Gly Tyr Lys Pro Ser Asp Tyr Val Ala Ser Arg Leu Trp Val Thr Ala 435 440 445 Lys Asp Gly Ala Gln Ile Pro Val Ser Leu Val His Arg Thr Asp Leu 450 455 460 Asp Val Ser Lys Pro Asn Pro Thr Leu Leu Tyr Gly Tyr Gly Ser Tyr 465 470 475 480 Glu Ser Ser Ile Asp Pro Gly Phe Ser Ile Ala Arg Leu Ser Leu Met 485 490 495 Asp Arg Gly Met Ile Phe Ala Ile Ala His Val Arg Gly Gly Gly Glu 500 505 510 Met Gly Arg Gly Trp Tyr Asp Asn Gly Lys Thr Thr Thr Lys Lys Asn 515 520 525 Thr Phe Thr Asp Phe Ile Asp Val Ala Asp Ala Leu Ile Glu Gln Lys 530 535 540 Ile Ser Ala Pro Glu Met Leu Val Ala Glu Gly Gly Ser Ala Gly Gly 545 550 555 560 Met Leu Met Gly Ala Ile Ala Asn Met Ala Gly Asp Arg Phe Lys Ala 565 570 575 Ile Glu Ala Asn Val Pro Phe Val Asp Pro Leu Thr Ser Met Leu Met 580 585 590 Pro Glu Leu Pro Leu Thr Val Ile Glu Trp Asp Glu Trp Gly Asp Pro 595 600 605 Leu His Asp Lys Asp Val Tyr Glu Tyr Met Ala Ser Tyr Ala Pro Tyr 610 615 620 Glu Asn Ile Glu Ala Lys Asn Tyr Pro Asn Ile Leu Ala Val Thr Ser 625 630 635 640 Leu Asn Asp Thr Arg Val Leu Tyr Val Glu Pro Ala Lys Trp Val Ala 645 650 655 Gln Leu Arg Ala Thr Ala Thr Gly Gly Glu Phe Leu Leu Lys Thr Glu 660 665 670 Met Val Ala Gly His Gly Gly Val Ser Gly Arg Tyr Glu Lys Trp Arg 675 680 685 Glu Thr Ala Phe Glu Tyr Gly Trp Leu Ile Asn Gln Ala Thr Gly Val 690 695 700 Thr glu 705 <210> 147 <211> 961 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (931) <223> RXA04098 <400> 147 acccgcagtt ctggctgagc cttcagactc agtacgagtt ggagctcgat cgcgacgccg 60 gtgcagcgac ttacgcacag atcacgccgc tgaaggtcgc gtg agc tcg gtg gcc 115 Val Ser Ser Val Ala 1 5 aga aaa cat ctg ctc tcc ctg gca gac tgg aac aga ggc gag ctt gag 163 Arg Lys His Leu Leu Ser Leu Ala Asp Trp Asn Arg Gly Glu Leu Glu 10 15 20 gca tta ttc gag ctc gcg gag cag tat gaa gct ggc ggt ggg cca cga 211 Ala Leu Phe Glu Leu Ala Glu Gln Tyr Glu Ala Gly Gly Gly Pro Arg 25 30 35 ttc gat ggt gcc gcg gcg atg ttc ttc ccg ccg acg agt ttg cgt aca 259 Phe Asp Gly Ala Ala Ala Met Phe Phe Pro Pro Thr Ser Leu Arg Thr 40 45 50 cgg ctc tca ttc gag cgt ggg gca acg gca atg gga ctc cag ccg atc 307 Arg Leu Ser Phe Glu Arg Gly Ala Thr Ala Met Gly Leu Gln Pro Ile 55 60 65 acg ttc ccg tca gac agc ctg gac aag gac gaa gat ctc gtc gac gtc 355 Thr Phe Pro Ser Asp Ser Leu Asp Lys Asp Glu Asp Leu Val Asp Val 70 75 80 85 gtc ggc tat ctc tcg cag tgg gct gat gtc gtt gtc gtc cga cac ccg 403 Val Gly Tyr Leu Ser Gln Trp Ala Asp Val Val Val Val Arg His Pro 90 95 100 caa ttg acg gcg ctt cag cgg ttg gcg tca gcg gat gca gcg ccc gtg 451 Gln Leu Thr Ala Leu Gln Arg Leu Ala Ser Ala Asp Ala Ala Pro Val 105 110 115 atc aac gcg atg acg agt gag aac cat ccg tgc gaa gtc ctc tcg gac 499 Ile Asn Ala Met Thr Ser Glu Asn His Pro Cys Glu Val Leu Ser Asp 120 125 130 ttg tat gcg ctg tct cgt cac cat gac att tcg gcc ctg cgg tac ctg 547 Leu Tyr Ala Leu Ser Arg His His Asp Ile Ser Ala Leu Arg Tyr Leu 135 140 145 ttt gtc ggt ggc gat ggc aac atc gcc agg gcc tgg tgg gag gcg gcc 595 Phe Val Gly Gly Asp Gly Asn Ile Ala Arg Ala Trp Trp Glu Ala Ala 150 155 160 165 caa gcg ttc ggc ctc gag atg cgg cag agt tgt cct gaa gag ctg cgt 643 Gln Ala Phe Gly Leu Glu Met Arg Gln Ser Cys Pro Glu Glu Leu Arg 170 175 180 gtc gtc ggg atg ccg tgg gag gag aac ctg ccg cat gca att gca tca 691 Val Val Gly Met Pro Trp Glu Glu Asn Leu Pro His Ala Ile Ala Ser 185 190 195 gcg gat gtc gtg ctg acg gat ggg cca ggt aga cat gcg gag tta ctc 739 Ala Asp Val Val Leu Thr Asp Gly Pro Gly Arg His Ala Glu Leu Leu 200 205 210 gag ccg tat cgt gtg acc gct gcg ttg ttg gat cgc gcg ccc cgt gga 787 Glu Pro Tyr Arg Val Thr Ala Ala Leu Leu Asp Arg Ala Pro Arg Gly 215 220 225 gtg cgg ctc gcg ccc tgc ccg ccg ttc atc cgc ggg cgc gaa gtg agc 835 Val Arg Leu Ala Pro Cys Pro Pro Phe Ile Arg Gly Arg Glu Val Ser 230 235 240 245 gcc gat gcg atc gag cat ccg gcg ttc gtc ggg tac tcg ttc aag cgt 883 Ala Asp Ala Ile Glu His Pro Ala Phe Val Gly Tyr Ser Phe Lys Arg 250 255 260 cat ctc atg ccg gtt cag cag gcg att ctg gct cgg tcg atc aat gcg 931 His Leu Met Pro Val Gln Gln Ala Ile Leu Ala Arg Ser Ile Asn Ala 265 270 275 tagcggtttt cggtgtcgga actcggtgga 961 <210> 148 <211> 277 <212> PRT <213> Corynebacterium glutamicum <400> 148 Val Ser Ser Val Ala Arg Lys His Leu Leu Ser Leu Ala Asp Trp Asn 1 5 10 15 Arg Gly Glu Leu Glu Ala Leu Phe Glu Leu Ala Glu Gln Tyr Glu Ala 20 25 30 Gly Gly Gly Pro Arg Phe Asp Gly Ala Ala Ala Met Phe Phe Pro Pro 35 40 45 Thr Ser Leu Arg Thr Arg Leu Ser Phe Glu Arg Gly Ala Thr Ala Met 50 55 60 Gly Leu Gln Pro Ile Thr Phe Pro Ser Asp Ser Leu Asp Lys Asp Glu 65 70 75 80 Asp Leu Val Asp Val Val Gly Tyr Leu Ser Gln Trp Ala Asp Val Val 85 90 95 Val Val Arg His Pro Gln Leu Thr Ala Leu Gln Arg Leu Ala Ser Ala 100 105 110 Asp Ala Ala Pro Val Ile Asn Ala Met Thr Ser Glu Asn His Pro Cys 115 120 125 Glu Val Leu Ser Asp Leu Tyr Ala Leu Ser Arg His His Asp Ile Ser 130 135 140 Ala Leu Arg Tyr Leu Phe Val Gly Gly Asp Gly Asn Ile Ala Arg Ala 145 150 155 160 Trp Trp Glu Ala Ala Gln Ala Phe Gly Leu Glu Met Arg Gln Ser Cys 165 170 175 Pro Glu Glu Leu Arg Val Val Gly Met Pro Trp Glu Glu Asn Leu Pro 180 185 190 His Ala Ile Ala Ser Ala Asp Val Val Leu Thr Asp Gly Pro Gly Arg 195 200 205 His Ala Glu Leu Leu Glu Pro Tyr Arg Val Thr Ala Ala Leu Leu Asp 210 215 220 Arg Ala Pro Arg Gly Val Arg Leu Ala Pro Cys Pro Pro Phe Ile Arg 225 230 235 240 Gly Arg Glu Val Ser Ala Asp Ala Ile Glu His Pro Ala Phe Val Gly 245 250 255 Tyr Ser Phe Lys Arg His Leu Met Pro Val Gln Gln Ala Ile Leu Ala 260 265 270 Arg Ser Ile Asn Ala 275 <210> 149 <211> 829 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (799) <223> RXA04205 <400> 149 gcttttgcag ctgtggggtt gggctggggg caagtgttca tgtaggtgtc attgatcgtt 60 cactcaaggt tggacgttgt gttcatgaat aacttttatg atg aac gca tcg tcc 115 Met Asn Ala Ser Ser 1 5 att tct tcc cga ttc aag gat ctt ttt gtg aca ccc agt att gtt ttt 163 Ile Ser Ser Arg Phe Lys Asp Leu Phe Val Thr Pro Ser Ile Val Phe 10 15 20 gat ttc gat ggc acg ctt gcc att ggc cat ggc cct gtc ctt gcg tat 211 Asp Phe Asp Gly Thr Leu Ala Ile Gly His Gly Pro Val Leu Ala Tyr 25 30 35 gca ttg tgt gtt gca ccg gag ggt tcc aag gac ttt ctg gag cgt gtg 259 Ala Leu Cys Val Ala Pro Glu Gly Ser Lys Asp Phe Leu Glu Arg Val 40 45 50 cgc agg gag ctt cgg cgc tat gac gat ggt cag agt att tac cgt gat 307 Arg Arg Glu Leu Arg Arg Tyr Asp Asp Gly Gln Ser Ile Tyr Arg Asp 55 60 65 ggg tat gac att gtg gct aag ttg gcg tcg gaa ttg ggg att gat gat 355 Gly Tyr Asp Ile Val Ala Lys Leu Ala Ser Glu Leu Gly Ile Asp Asp 70 75 80 85 ggc acg atg tct gtc gct tat ggc gag agc cgg aag ttg ctt ggt tcg 403 Gly Thr Met Ser Val Ala Tyr Gly Glu Ser Arg Lys Leu Leu Gly Ser 90 95 100 gat tta gcg cct gtt gag cat gtg cgg ggt att aag gat att ttg tcc 451 Asp Leu Ala Pro Val Glu His Val Arg Gly Ile Lys Asp Ile Leu Ser 105 110 115 tcg ttg aaa ggt cat gct cgg ttg gtt tta gct acc aat gcc ccg gaa 499 Ser Leu Lys Gly His Ala Arg Leu Val Leu Ala Thr Asn Ala Pro Glu 120 125 130 aat ggc gtg cat gat ttg ctg cgt cag tgg ggt gtt gct gat ttg ttt 547 Asn Gly Val His Asp Leu Leu Arg Gln Trp Gly Val Ala Asp Leu Phe 135 140 145 gat cag ttg cat ttt gtg gtg ggt aag cct gca ggg ttg att tcg atc 595 Asp Gln Leu His Phe Val Val Gly Lys Pro Ala Gly Leu Ile Ser Ile 150 155 160 165 att tct gat ttg cag ctt gat ggt ccg gtg ctt gcg gtg ggc gat att 643 Ile Ser Asp Leu Gln Leu Asp Gly Pro Val Leu Ala Val Gly Asp Ile 170 175 180 tat gaa ttc gat ctg agt cct gca gcg cag tta ggt gca gat acg gct 691 Tyr Glu Phe Asp Leu Ser Pro Ala Ala Gln Leu Gly Ala Asp Thr Ala 185 190 195 ctg gtt gga gct aca gca acc att tct gaa gcg aag gtc tcc atg cgt 739 Leu Val Gly Ala Thr Ala Thr Ile Ser Glu Ala Lys Val Ser Met Arg 200 205 210 gga gat tct atc gct gat ctc cct ctc ctt gcc tgg gtt tgt tcc cgg 787 Gly Asp Ser Ile Ala Asp Leu Pro Leu Leu Ala Trp Val Cys Ser Arg 215 220 225 gtc tca tct tct taactttctt gtctcatgtc gctgaaaggt 829 Val Ser Ser Ser 230 <210> 150 <211> 233 <212> PRT <213> Corynebacterium glutamicum <400> 150 Met Asn Ala Ser Ser Ile Ser Ser Arg Phe Lys Asp Leu Phe Val Thr 1 5 10 15 Pro Ser Ile Val Phe Asp Phe Asp Gly Thr Leu Ala Ile Gly His Gly 20 25 30 Pro Val Leu Ala Tyr Ala Leu Cys Val Ala Pro Glu Gly Ser Lys Asp 35 40 45 Phe Leu Glu Arg Val Arg Arg Glu Leu Arg Arg Tyr Asp Asp Gly Gln 50 55 60 Ser Ile Tyr Arg Asp Gly Tyr Asp Ile Val Ala Lys Leu Ala Ser Glu 65 70 75 80 Leu Gly Ile Asp Asp Gly Thr Met Ser Val Ala Tyr Gly Glu Ser Arg 85 90 95 Lys Leu Leu Gly Ser Asp Leu Ala Pro Val Glu His Val Arg Gly Ile 100 105 110 Lys Asp Ile Leu Ser Ser Leu Lys Gly His Ala Arg Leu Val Leu Ala 115 120 125 Thr Asn Ala Pro Glu Asn Gly Val His Asp Leu Leu Arg Gln Trp Gly 130 135 140 Val Ala Asp Leu Phe Asp Gln Leu His Phe Val Val Gly Lys Pro Ala 145 150 155 160 Gly Leu Ile Ser Ile Ile Ser Asp Leu Gln Leu Asp Gly Pro Val Leu 165 170 175 Ala Val Gly Asp Ile Tyr Glu Phe Asp Leu Ser Pro Ala Ala Gln Leu 180 185 190 Gly Ala Asp Thr Ala Leu Val Gly Ala Thr Ala Thr Ile Ser Glu Ala 195 200 205 Lys Val Ser Met Arg Gly Asp Ser Ile Ala Asp Leu Pro Leu Leu Ala 210 215 220 Trp Val Cys Ser Arg Val Ser Ser Ser 225 230 <210> 151 <211> 1609 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1579) <223> RXA04381 <400> 151 acctgtgtca cggacctgcg cctgacctga tgcctcagca gcgggtttcc cctgatcctt 60 cccctgtgtc actcagactt gcaccaagga cggtttaacc atg aca agt agt ttt 115 Met Thr Ser Ser Phe 1 5 tcc cgg cga cag ttt ctg ctc ggc ggg ctc gta ctc gcc ggc acc ggg 163 Ser Arg Arg Gln Phe Leu Leu Gly Gly Leu Val Leu Ala Gly Thr Gly 10 15 20 gcc gtg gcc gcc tgc acc agc gac cct gga ccc gct gcc tcg gca cca 211 Ala Val Ala Ala Cys Thr Ser Asp Pro Gly Pro Ala Ala Ser Ala Pro 25 30 35 ggt ccc tcc ctt cgc ccc act ccc acc ccc act gcg ctc ggt gag ccg 259 Gly Pro Ser Leu Arg Pro Thr Pro Thr Pro Thr Ala Leu Gly Glu Pro 40 45 50 acg gtg cgc cgg aca ctg acc gcc cgg ccc ctc tcc ctg gat atc ggc 307 Thr Val Arg Arg Thr Leu Thr Ala Arg Pro Leu Ser Leu Asp Ile Gly 55 60 65 ggc atc gaa gcc aag acg tgg gga tac gtc tct gac acc ggg gat gcg 355 Gly Ile Glu Ala Lys Thr Trp Gly Tyr Val Ser Asp Thr Gly Asp Ala 70 75 80 85 gcc att gag gcc acc gcc ggc gac gtc ctc cag gtc gat atc acc aat 403 Ala Ile Glu Ala Thr Ala Gly Asp Val Leu Gln Val Asp Ile Thr Asn 90 95 100 gac ctg cct gag agc acc tcc atc cac tgg cat ggc atc gca ctc cac 451 Asp Leu Pro Glu Ser Thr Ser Ile His Trp His Gly Ile Ala Leu His 105 110 115 aac gca gcc gac ggt gtg ccc ggc atg acc cag gac ccc att gaa cct 499 Asn Ala Ala Asp Gly Val Pro Gly Met Thr Gln Asp Pro Ile Glu Pro 120 125 130 ggc gag tct ttc tcc tat gtt ttt gaa gtc ccc cac ggt ggc acc tac 547 Gly Glu Ser Phe Ser Tyr Val Phe Glu Val Pro His Gly Gly Thr Tyr 135 140 145 ttc tac cat tcc cac acc ggc ctg cag ctt gat cgc ggc ctc cac gcc 595 Phe Tyr His Ser His Thr Gly Leu Gln Leu Asp Arg Gly Leu His Ala 150 155 160 165 cca ctg atc atc cgt gac ccg caa gac gct gag gac cag gac gtc gag 643 Pro Leu Ile Ile Arg Asp Pro Gln Asp Ala Glu Asp Gln Asp Val Glu 170 175 180 tgg acc atc gtg ctc gac gac tgg gtc gat ggc att cag ggc act ccc 691 Trp Thr Ile Val Leu Asp Asp Trp Val Asp Gly Ile Gln Gly Thr Pro 185 190 195 gac gat gag ctc gac aag ctc acc gga atg ggt tcg ggc gac cat aac 739 Asp Asp Glu Leu Asp Lys Leu Thr Gly Met Gly Ser Gly Asp His Asn 200 205 210 ggg agg atg gga atg gga ggt cac ggc cag atg atg cac ggc acc ccg 787 Gly Arg Met Gly Met Gly Gly His Gly Gln Met Met His Gly Thr Pro 215 220 225 gac cgg gta ctg ggc ggg gat gtc ggc gat gtg atg tat ccg cac tac 835 Asp Arg Val Leu Gly Gly Asp Val Gly Asp Val Met Tyr Pro His Tyr 230 235 240 245 ctc atc aac gga cgt atc ccc cgt gct cac cgg acc ttc gag gct cgc 883 Leu Ile Asn Gly Arg Ile Pro Arg Ala His Arg Thr Phe Glu Ala Arg 250 255 260 ccg ggc gac aag gcc cgc ctg cgg ttt atc aac tcc ggc ggt gac acc 931 Pro Gly Asp Lys Ala Arg Leu Arg Phe Ile Asn Ser Gly Gly Asp Thr 265 270 275 atc ttc aag gtg gcc ctc ggt ggt cac cgc atg act gtc acc cac acc 979 Ile Phe Lys Val Ala Leu Gly Gly His Arg Met Thr Val Thr His Thr 280 285 290 gac ggc ttc cct gtc cag ccc tgg gag acc gaa tcg atc tac ctg tcg 1027 Asp Gly Phe Pro Val Gln Pro Trp Glu Thr Glu Ser Ile Tyr Leu Ser 295 300 305 atg ggc gag cgt gtc gac gtc gag gtc atc ctc ggc gac ggc atc ttc 1075 Met Gly Glu Arg Val Asp Val Glu Val Ile Leu Gly Asp Gly Ile Phe 310 315 320 325 ccg ctc acg gct ttg gcg gtg ggt aag gac gac cgc gcc ttc gcc gtc 1123 Pro Leu Thr Ala Leu Ala Val Gly Lys Asp Asp Arg Ala Phe Ala Val 330 335 340 atc cgc acc gcc ggc ggc cag gcc ccc cgc ccc gat gtc gac ttc ccc 1171 Ile Arg Thr Ala Gly Gly Gln Ala Pro Arg Pro Asp Val Asp Phe Pro 345 350 355 gag ttg tcg tcc acc gga ctg ctt ctg tcc tcc ctg aag cca gca gac 1219 Glu Leu Ser Ser Thr Gly Leu Leu Leu Ser Ser Leu Lys Pro Ala Asp 360 365 370 cgt gca ctc ctg ccc gag ggc aca cca gac cga gaa gtc agc atc gac 1267 Arg Ala Leu Leu Pro Glu Gly Thr Pro Asp Arg Glu Val Ser Ile Asp 375 380 385 ctg ggc ggg cag atg atg ccg tat gaa tgg agc att ctc acc gac ggc 1315 Leu Gly Gly Gln Met Met Pro Tyr Glu Trp Ser Ile Leu Thr Asp Gly 390 395 400 405 caa tcc tcc tcc gcg acc gtg cag gag ggc cag cgc ctg cgg atg gtc 1363 Gln Ser Ser Ser Ala Thr Val Gln Glu Gly Gln Arg Leu Arg Met Val 410 415 420 atg cgc aac agg acc atg atg ccc cat ccc atg cac atc cac ggc cac 1411 Met Arg Asn Arg Thr Met Met Pro His Pro Met His Ile His Gly His 425 430 435 acg tgg gcg ctg ccc ggc agc ggc ggg cta cgc aag gac acc gtc ctt 1459 Thr Trp Ala Leu Pro Gly Ser Gly Gly Leu Arg Lys Asp Thr Val Leu 440 445 450 ctc cgc cac ggt gaa acc atg atc gcc gac ctg atc gct gac aac ccc 1507 Leu Arg His Gly Glu Thr Met Ile Ala Asp Leu Ile Ala Asp Asn Pro 455 460 465 ggt gag tgg gca ttt cac tgc cat aac gcc tat cac atg gaa acc ggg 1555 Gly Glu Trp Ala Phe His Cys His Asn Ala Tyr His Met Glu Thr Gly 470 475 480 485 atg gtc agc tcg ctt cgc tac gag taacccccac agcagggttg agtgccgagg 1609 Met Val Ser Ser Leu Arg Tyr Glu 490 <210> 152 <211> 493 <212> PRT <213> Corynebacterium glutamicum <400> 152 Met Thr Ser Ser Phe Ser Arg Arg Gln Phe Leu Leu Gly Gly Leu Val 1 5 10 15 Leu Ala Gly Thr Gly Ala Val Ala Ala Cys Thr Ser Asp Pro Gly Pro 20 25 30 Ala Ala Ser Ala Pro Gly Pro Ser Leu Arg Pro Thr Pro Thr Pro Thr 35 40 45 Ala Leu Gly Glu Pro Thr Val Arg Arg Thr Leu Thr Ala Arg Pro Leu 50 55 60 Ser Leu Asp Ile Gly Gly Ile Glu Ala Lys Thr Trp Gly Tyr Val Ser 65 70 75 80 Asp Thr Gly Asp Ala Ala Ile Glu Ala Thr Ala Gly Asp Val Leu Gln 85 90 95 Val Asp Ile Thr Asn Asp Leu Pro Glu Ser Thr Ser Ile His Trp His 100 105 110 Gly Ile Ala Leu His Asn Ala Ala Asp Gly Val Pro Gly Met Thr Gln 115 120 125 Asp Pro Ile Glu Pro Gly Glu Ser Phe Ser Tyr Val Phe Glu Val Pro 130 135 140 His Gly Gly Thr Tyr Phe Tyr His Ser His Thr Gly Leu Gln Leu Asp 145 150 155 160 Arg Gly Leu His Ala Pro Leu Ile Ile Arg Asp Pro Gln Asp Ala Glu 165 170 175 Asp Gln Asp Val Glu Trp Thr Ile Val Leu Asp Asp Trp Val Asp Gly 180 185 190 Ile Gln Gly Thr Pro Asp Asp Glu Leu Asp Lys Leu Thr Gly Met Gly 195 200 205 Ser Gly Asp His Asn Gly Arg Met Gly Met Gly Gly His Gly Gln Met 210 215 220 Met His Gly Thr Pro Asp Arg Val Leu Gly Gly Asp Val Gly Asp Val 225 230 235 240 Met Tyr Pro His Tyr Leu Ile Asn Gly Arg Ile Pro Arg Ala His Arg 245 250 255 Thr Phe Glu Ala Arg Pro Gly Asp Lys Ala Arg Leu Arg Phe Ile Asn 260 265 270 Ser Gly Gly Asp Thr Ile Phe Lys Val Ala Leu Gly Gly His Arg Met 275 280 285 Thr Val Thr His Thr Asp Gly Phe Pro Val Gln Pro Trp Glu Thr Glu 290 295 300 Ser Ile Tyr Leu Ser Met Gly Glu Arg Val Asp Val Glu Val Ile Leu 305 310 315 320 Gly Asp Gly Ile Phe Pro Leu Thr Ala Leu Ala Val Gly Lys Asp Asp 325 330 335 Arg Ala Phe Ala Val Ile Arg Thr Ala Gly Gly Gln Ala Pro Arg Pro 340 345 350 Asp Val Asp Phe Pro Glu Leu Ser Ser Thr Gly Leu Leu Leu Ser Ser 355 360 365 Leu Lys Pro Ala Asp Arg Ala Leu Leu Pro Glu Gly Thr Pro Asp Arg 370 375 380 Glu Val Ser Ile Asp Leu Gly Gly Gln Met Met Pro Tyr Glu Trp Ser 385 390 395 400 Ile Leu Thr Asp Gly Gln Ser Ser Ser Ala Thr Val Gln Glu Gly Gln 405 410 415 Arg Leu Arg Met Val Met Arg Asn Arg Thr Met Met Pro His Pro Met 420 425 430 His Ile His Gly His Thr Trp Ala Leu Pro Gly Ser Gly Gly Leu Arg 435 440 445 Lys Asp Thr Val Leu Leu Arg His Gly Glu Thr Met Ile Ala Asp Leu 450 455 460 Ile Ala Asp Asn Pro Gly Glu Trp Ala Phe His Cys His Asn Ala Tyr 465 470 475 480 His Met Glu Thr Gly Met Val Ser Ser Leu Arg Tyr Glu 485 490 <210> 153 <211> 1072 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101). (1042) <223> RXA04540 <400> 153 tgagtcggta gaagtattac ccagtgactt aagtttcttc agcatttttt tgagcaacag 60 cgaccagcca cgttagtgtg gtcgagtaga ggatagctac atg ggg aac tgg gca 115 Met Gly Asn Trp Ala 1 5 gag att act gat gaa att tct aag att tac caa gat aat cag tac aag 163 Glu Ile Thr Asp Glu Ile Ser Lys Ile Tyr Gln Asp Asn Gln Tyr Lys 10 15 20 att aga caa ata aat gat gtt gac gca gta agc gat aaa cgt aga gaa 211 Ile Arg Gln Ile Asn Asp Val Asp Ala Val Ser Asp Lys Arg Arg Glu 25 30 35 gcg cta caa gca ctg ttt gaa cat act ggt cga aat gta atc gtc tat 259 Ala Leu Gln Ala Leu Phe Glu His Thr Gly Arg Asn Val Ile Val Tyr 40 45 50 tat tca gcg tgg tta gaa aat ggt cga cga ttt tcc ggg caa tct acg 307 Tyr Ser Ala Trp Leu Glu Asn Gly Arg Arg Phe Ser Gly Gln Ser Thr 55 60 65 gat ttt tcg gta aat gat act gat aaa aac agt ttt atg act gcg ctc 355 Asp Phe Ser Val Asn Asp Thr Asp Lys Asn Ser Phe Met Thr Ala Leu 70 75 80 85 cat aag ttg gat cag agt aaa ggt ctc gat ctt atc ctc cac act ccg 403 His Lys Leu Asp Gln Ser Lys Gly Leu Asp Leu Ile Leu His Thr Pro 90 95 100 ggt gga gat gtt gct gcg aca gag tcg tta gta gat tac att cac gca 451 Gly Gly Asp Val Ala Ala Thr Glu Ser Leu Val Asp Tyr Ile His Ala 105 110 115 ctc ttt ggt caa gat ttc aga gtc att gtc ccc caa ctc gca atg tca 499 Leu Phe Gly Gln Asp Phe Arg Val Ile Val Pro Gln Leu Ala Met Ser 120 125 130 gca gga aca atg atc gca ctt tcg tcc aaa gag att gtt atg ggg aag 547 Ala Gly Thr Met Ile Ala Leu Ser Ser Lys Glu Ile Val Met Gly Lys 135 140 145 cat tct agt ctt ggc ccc att gat cct cag ttt aac ggc cta ccg gca 595 His Ser Ser Leu Gly Pro Ile Asp Pro Gln Phe Asn Gly Leu Pro Ala 150 155 160 165 cac ggg tta ttg gaa gaa ttt gag caa gcg aag aaa gag gtc tct gag 643 His Gly Leu Leu Glu Glu Phe Glu Gln Ala Lys Lys Glu Val Ser Glu 170 175 180 aat ccg cag act gct cat ata tgg cag gtg atc ttg aat aaa tac aac 691 Asn Pro Gln Thr Ala His Ile Trp Gln Val Ile Leu Asn Lys Tyr Asn 185 190 195 ccc acg atg ttg ggt gaa gct aaa aaa gct att cag tgg tcc aac tcg 739 Pro Thr Met Leu Gly Glu Ala Lys Lys Ala Ile Gln Trp Ser Asn Ser 200 205 210 atg gtt aag cag tgg ctt gaa aag ggt atg ttt tta gac gag cct gac 787 Met Val Lys Gln Trp Leu Glu Lys Gly Met Phe Leu Asp Glu Pro Asp 215 220 225 aaa gaa gaa aaa gcc act cgc gct atc aaa gag ctc gct gat cat tcc 835 Lys Glu Glu Lys Ala Thr Arg Ala Ile Lys Glu Leu Ala Asp His Ser 230 235 240 245 gtt act ctt gcg cat aat cga cac att tcg gtc agt aaa gca ctt gag 883 Val Thr Leu Ala His Asn Arg His Ile Ser Val Ser Lys Ala Leu Glu 250 255 260 ctg gga ttg aat atc aaa gaa ctt gag agc gat cca aag ctt caa gat 931 Leu Gly Leu Asn Ile Lys Glu Leu Glu Ser Asp Pro Lys Leu Gln Asp 265 270 275 tta gtt ctt act ctt cac cac ctg tcc gtt att gct gcg caa cga gga 979 Leu Val Leu Thr Leu His His Leu Ser Val Ile Ala Ala Gln Arg Gly 280 285 290 cca tta att aag ttt gtc gtc aat cat gac aac cgt ggc act ttt ctg 1027 Pro Leu Ile Lys Phe Val Val Asn His Asp Asn Arg Gly Thr Phe Leu 295 300 305 cag ggg cat gaa aac taattaagtg atgcaatagt ctaccgtgtg 1072 Gln Gly His Glu Asn 310 <210> 154 <211> 314 <212> PRT <213> Corynebacterium glutamicum <400> 154 Met Gly Asn Trp Ala Glu Ile Thr Asp Glu Ile Ser Lys Ile Tyr Gln 1 5 10 15 Asp Asn Gln Tyr Lys Ile Arg Gln Ile Asn Asp Val Asp Ala Val Ser 20 25 30 Asp Lys Arg Arg Glu Ala Leu Gln Ala Leu Phe Glu His Thr Gly Arg 35 40 45 Asn Val Ile Val Tyr Tyr Ser Ala Trp Leu Glu Asn Gly Arg Arg Phe 50 55 60 Ser Gly Gln Ser Thr Asp Phe Ser Val Asn Asp Thr Asp Lys Asn Ser 65 70 75 80 Phe Met Thr Ala Leu His Lys Leu Asp Gln Ser Lys Gly Leu Asp Leu 85 90 95 Ile Leu His Thr Pro Gly Gly Asp Val Ala Ala Thr Glu Ser Leu Val 100 105 110 Asp Tyr Ile His Ala Leu Phe Gly Gln Asp Phe Arg Val Ile Val Pro 115 120 125 Gln Leu Ala Met Ser Ala Gly Thr Met Ile Ala Leu Ser Ser Lys Glu 130 135 140 Ile Val Met Gly Lys His Ser Ser Leu Gly Pro Ile Asp Pro Gln Phe 145 150 155 160 Asn Gly Leu Pro Ala His Gly Leu Leu Glu Glu Phe Glu Gln Ala Lys 165 170 175 Lys Glu Val Ser Glu Asn Pro Gln Thr Ala His Ile Trp Gln Val Ile 180 185 190 Leu Asn Lys Tyr Asn Pro Thr Met Leu Gly Glu Ala Lys Lys Ala Ile 195 200 205 Gln Trp Ser Asn Ser Met Val Lys Gln Trp Leu Glu Lys Gly Met Phe 210 215 220 Leu Asp Glu Pro Asp Lys Glu Glu Lys Ala Thr Arg Ala Ile Lys Glu 225 230 235 240 Leu Ala Asp His Ser Val Thr Leu Ala His Asn Arg His Ile Ser Val 245 250 255 Ser Lys Ala Leu Glu Leu Gly Leu Asn Ile Lys Glu Leu Glu Ser Asp 260 265 270 Pro Lys Leu Gln Asp Leu Val Leu Thr Leu His His Leu Ser Val Ile 275 280 285 Ala Ala Gln Arg Gly Pro Leu Ile Lys Phe Val Val Asn His Asp Asn 290 295 300 Arg Gly Thr Phe Leu Gln Gly His Glu Asn 305 310 <210> 155 <211> 1411 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1381) <223> RXA04618 <400> 155 aatttgagag ccccgtggtt ccatcgtccc cggttcgcgt atagatcttg gttaaatgaa 60 cggccataac ttgagattct agctgtcgtt acgcacaggg gtg ggg aac acg gat 115 Val Gly Asn Thr Asp 1 5 aag gtg ggg cac gtg aaa gac aag ttt tta gtc act ggt gga gca cag 163 Lys Val Gly His Val Lys Asp Lys Phe Leu Val Thr Gly Gly Ala Gln 10 15 20 ctg cag ggc gct gta aaa gtt tac ggc gca aaa aac agc gtt ttg aag 211 Leu Gln Gly Ala Val Lys Val Tyr Gly Ala Lys Asn Ser Val Leu Lys 25 30 35 ctc atg gca gca gca ctt ctc gct gaa ggc aca aca act cta acc aat 259 Leu Met Ala Ala Ala Leu Leu Ala Glu Gly Thr Thr Thr Leu Thr Asn 40 45 50 tgc ccc gaa atc ctc gac gtc ccc ctg atg cgc gac gtc ctc gtt ggt 307 Cys Pro Glu Ile Leu Asp Val Pro Leu Met Arg Asp Val Leu Val Gly 55 60 65 ctt ggc tgc gat gtc acc atc gac ggc tca acc gta acc att act acc 355 Leu Gly Cys Asp Val Thr Ile Asp Gly Ser Thr Val Thr Ile Thr Thr 70 75 80 85 cct gca gaa ctc agc tcc aat gct gac ttc cca gca gtc acc caa ttc 403 Pro Ala Glu Leu Ser Ser Asn Ala Asp Phe Pro Ala Val Thr Gln Phe 90 95 100 cgt gca tcc gta tgt gtg ctt ggt cca ttg aca gca cgt tgt ggt cgc 451 Arg Ala Ser Val Cys Val Leu Gly Pro Leu Thr Ala Arg Cys Gly Arg 105 110 115 gca gtt gta tcc ctt ccc ggc ggt gac gcc att gga tcc cgt cca ctc 499 Ala Val Val Ser Leu Pro Gly Gly Asp Ala Ile Gly Ser Arg Pro Leu 120 125 130 gac atg cat caa agc ggc ctg gaa aag ctt ggt gcc acc acc cgc att 547 Asp Met His Gln Ser Gly Leu Glu Lys Leu Gly Ala Thr Thr Arg Ile 135 140 145 tcc cac ggt gca gta gtt gca gaa gct gaa aag ctc gtc ggt gcc aac 595 Ser His Gly Ala Val Val Ala Glu Ala Glu Lys Leu Val Gly Ala Asn 150 155 160 165 atc acc ctg gat ttc ccg tcc gtc ggc gcc acc gaa aac atc ctc act 643 Ile Thr Leu Asp Phe Pro Ser Val Gly Ala Thr Glu Asn Ile Leu Thr 170 175 180 gca tcc gtc atg gca gaa gga cgc aca gta tta gat aac gca gcg cgc 691 Ala Ser Val Met Ala Glu Gly Arg Thr Val Leu Asp Asn Ala Ala Arg 185 190 195 gaa cca gaa att gtt gat ctc tgc cgt atg ctt cga tcc atg ggc gcc 739 Glu Pro Glu Ile Val Asp Leu Cys Arg Met Leu Arg Ser Met Gly Ala 200 205 210 aac att gaa ggt gaa gga agc cca acc atc acc atc gaa ggc gta gag 787 Asn Ile Glu Gly Glu Gly Ser Pro Thr Ile Thr Ile Glu Gly Val Glu 215 220 225 aaa ctc acc cca act cag cac gaa gta atc ggc gac cgc atc gtt gcc 835 Lys Leu Thr Pro Thr Gln His Glu Val Ile Gly Asp Arg Ile Val Ala 230 235 240 245 gga acg tgg gca tac gcc gct gcg atg act cgt ggc gat att aca gtt 883 Gly Thr Trp Ala Tyr Ala Ala Ala Met Thr Arg Gly Asp Ile Thr Val 250 255 260 ggc gga atc gca cca agg tat ctg cac ctt cca ttg gaa aag ctc aag 931 Gly Gly Ile Ala Pro Arg Tyr Leu His Leu Pro Leu Glu Lys Leu Lys 265 270 275 atc gcc ggc gcc aag gtg gaa acc tac gaa aac ggc ttc cgc gtc caa 979 Ile Ala Gly Ala Lys Val Glu Thr Tyr Glu Asn Gly Phe Arg Val Gln 280 285 290 atg gat aag cag cct gag gca acc gac tac caa acc ctc ccg ttc cca 1027 Met Asp Lys Gln Pro Glu Ala Thr Asp Tyr Gln Thr Leu Pro Phe Pro 295 300 305 ggg ttc cct aca gat ctg caa ccc atg gca att gga atc aac gca gta 1075 Gly Phe Pro Thr Asp Leu Gln Pro Met Ala Ile Gly Ile Asn Ala Val 310 315 320 325 tct aat gga act tca gta att aca gag aat gtc ttt gaa tca cga ttc 1123 Ser Asn Gly Thr Ser Val Ile Thr Glu Asn Val Phe Glu Ser Arg Phe 330 335 340 cgc ttc gtc gat gaa atg ctt cgc ctg ggc gct gac gcg aat gtc gat 1171 Arg Phe Val Asp Glu Met Leu Arg Leu Gly Ala Asp Ala Asn Val Asp 345 350 355 ggg cac cac gta gta atc cga gga att gaa cag ctt tcc tct act tcc 1219 Gly His His Val Val Ile Arg Gly Ile Glu Gln Leu Ser Ser Thr Ser 360 365 370 gtg tgg tct tca gat atc cgt gca gga gca gga ctg gtt ctt gcc gcc 1267 Val Trp Ser Ser Asp Ile Arg Ala Gly Ala Gly Leu Val Leu Ala Ala 375 380 385 ctt tgc gca gac gga gtg acc gaa gtt cac gat gtt ttc cac atc gac 1315 Leu Cys Ala Asp Gly Val Thr Glu Val His Asp Val Phe His Ile Asp 390 395 400 405 cgc gga tac ccc aac ttc gtg gaa aat ctg cag aaa ctc gga gcg acc 1363 Arg Gly Tyr Pro Asn Phe Val Glu Asn Leu Gln Lys Leu Gly Ala Thr 410 415 420 atc gaa agg gtt tct tcc taacgaagcc ttcccatcaa gcatccccca 1411 Ile Glu Arg Val Ser Ser 425 <210> 156 <211> 427 <212> PRT <213> Corynebacterium glutamicum <400> 156 Val Gly Asn Thr Asp Lys Val Gly His Val Lys Asp Lys Phe Leu Val 1 5 10 15 Thr Gly Gly Ala Gln Leu Gln Gly Ala Val Lys Val Tyr Gly Ala Lys 20 25 30 Asn Ser Val Leu Lys Leu Met Ala Ala Ala Leu Leu Ala Glu Gly Thr 35 40 45 Thr Thr Leu Thr Asn Cys Pro Glu Ile Leu Asp Val Pro Leu Met Arg 50 55 60 Asp Val Leu Val Gly Leu Gly Cys Asp Val Thr Ile Asp Gly Ser Thr 65 70 75 80 Val Thr Ile Thr Thr Pro Ala Glu Leu Ser Ser Asn Ala Asp Phe Pro 85 90 95 Ala Val Thr Gln Phe Arg Ala Ser Val Cys Val Leu Gly Pro Leu Thr 100 105 110 Ala Arg Cys Gly Arg Ala Val Val Ser Leu Pro Gly Gly Asp Ala Ile 115 120 125 Gly Ser Arg Pro Leu Asp Met His Gln Ser Gly Leu Glu Lys Leu Gly 130 135 140 Ala Thr Thr Arg Ile Ser His Gly Ala Val Val Ala Glu Ala Glu Lys 145 150 155 160 Leu Val Gly Ala Asn Ile Thr Leu Asp Phe Pro Ser Val Gly Ala Thr 165 170 175 Glu Asn Ile Leu Thr Ala Ser Val Met Ala Glu Gly Arg Thr Val Leu 180 185 190 Asp Asn Ala Ala Arg Glu Pro Glu Ile Val Asp Leu Cys Arg Met Leu 195 200 205 Arg Ser Met Gly Ala Asn Ile Glu Gly Glu Gly Ser Pro Thr Ile Thr 210 215 220 Ile Glu Gly Val Glu Lys Leu Thr Pro Thr Gln His Glu Val Ile Gly 225 230 235 240 Asp Arg Ile Val Ala Gly Thr Trp Ala Tyr Ala Ala Ala Met Thr Arg 245 250 255 Gly Asp Ile Thr Val Gly Gly Ile Ala Pro Arg Tyr Leu His Leu Pro 260 265 270 Leu Glu Lys Leu Lys Ile Ala Gly Ala Lys Val Glu Thr Tyr Glu Asn 275 280 285 Gly Phe Arg Val Gln Met Asp Lys Gln Pro Glu Ala Thr Asp Tyr Gln 290 295 300 Thr Leu Pro Phe Pro Gly Phe Pro Thr Asp Leu Gln Pro Met Ala Ile 305 310 315 320 Gly Ile Asn Ala Val Ser Asn Gly Thr Ser Val Ile Thr Glu Asn Val 325 330 335 Phe Glu Ser Arg Phe Arg Phe Val Asp Glu Met Leu Arg Leu Gly Ala 340 345 350 Asp Ala Asn Val Asp Gly His His Val Val Ile Arg Gly Ile Glu Gln 355 360 365 Leu Ser Ser Thr Ser Val Trp Ser Ser Asp Ile Arg Ala Gly Ala Gly 370 375 380 Leu Val Leu Ala Ala Leu Cys Ala Asp Gly Val Thr Glu Val His Asp 385 390 395 400 Val Phe His Ile Asp Arg Gly Tyr Pro Asn Phe Val Glu Asn Leu Gln 405 410 415 Lys Leu Gly Ala Thr Ile Glu Arg Val Ser Ser 420 425 <210> 157 <211> 1099 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (101) .. (1069) <223> RXA06030 <400> 157 ggtagttggc gcagcagcag ctccatttgt catgggtctc accgattcca ccgcgcttcg 60 cttcctcctg gcgatcggca ccgtggtgct gctggttggt ttg gga aat ctc atc 115 Leu Gly Asn Leu Ile 1 5 ggc gcc cac ttg ggt gct gcg att aga gac aac atc aaa ttc cga agt 163 Gly Ala His Leu Gly Ala Ala Ile Arg Asp Asn Ile Lys Phe Arg Ser 10 15 20 tcc agg acc tta gat tct ggg ctc ggc gcc att ttc caa gta ttg gcc 211 Ser Arg Thr Leu Asp Ser Gly Leu Gly Ala Ile Phe Gln Val Leu Ala 25 30 35 acc ttg atc gtg gtg tgg ctc gtc gca att ccc ctg gcc aca ggc ctc 259 Thr Leu Ile Val Val Trp Leu Val Ala Ile Pro Leu Ala Thr Gly Leu 40 45 50 ccc gga act gtc gcc agc gga att aga gac tcc cgc atc ctg ggc ttt 307 Pro Gly Thr Val Ala Ser Gly Ile Arg Asp Ser Arg Ile Leu Gly Phe 55 60 65 gta gac aaa tac acc ccg caa ggc cta gat acc ctg ccc tcc aaa atc 355 Val Asp Lys Tyr Thr Pro Gln Gly Leu Asp Thr Leu Pro Ser Lys Ile 70 75 80 85 gct gcg atg ctc agc gaa tcc ggc ctc cca cca ctg att tcc ccc ttc 403 Ala Ala Met Leu Ser Glu Ser Gly Leu Pro Pro Leu Ile Ser Pro Phe 90 95 100 acc ggc gga tcc tcg gtg gaa gtg gac gcc ccc gaa atc aac gtc acc 451 Thr Gly Gly Ser Ser Val Glu Val Asp Ala Pro Glu Ile Asn Val Thr 105 110 115 aac gtt gac cta gtc gaa gca atg cgc ccg tcc gtc atc cac gtg atg 499 Asn Val Asp Leu Val Glu Ala Met Arg Pro Ser Val Ile His Val Met 120 125 130 ggt gac gcc caa gaa tgc agc cgc cga ctc atg ggt tct ggc ttt gtg 547 Gly Asp Ala Gln Glu Cys Ser Arg Arg Leu Met Gly Ser Gly Phe Val 135 140 145 gca tcc ccc gac tac gtt gtg acc aac gcc cac gtt gtt gca ggt acc 595 Ala Ser Pro Asp Tyr Val Val Thr Asn Ala His Val Val Ala Gly Thr 150 155 160 165 tcc acc gtc agc ctg gat acc atg atc gga acc cgc tcc gca gag gta 643 Ser Thr Val Ser Leu Asp Thr Met Ile Gly Thr Arg Ser Ala Glu Val 170 175 180 gtg ttc tac gac ccg aac ctg gac atc gca gtc ctt tac agc cct gac 691 Val Phe Tyr Asp Pro Asn Leu Asp Ile Ala Val Leu Tyr Ser Pro Asp 185 190 195 ctc ggc ttg gat cca ctg ccg tgg gca tcc act ccg cta gac act ggc 739 Leu Gly Leu Asp Pro Leu Pro Trp Ala Ser Thr Pro Leu Asp Thr Gly 200 205 210 gat gaa gca atc gtc atg gga ttc cca cag tcc gga cct ttc aac gcc 787 Asp Glu Ala Ile Val Met Gly Phe Pro Gln Ser Gly Pro Phe Asn Ala 215 220 225 tcc cca gcc agg gtc cgc gaa cgc atc atg atc acc ggc agc aac att 835 Ser Pro Ala Arg Val Arg Glu Arg Ile Met Ile Thr Gly Ser Asn Ile 230 235 240 245 tac gcc aac ggc cag cac gaa cgc gaa gcc tat tca gtc cgc gga tcc 883 Tyr Ala Asn Gly Gln His Glu Arg Glu Ala Tyr Ser Val Arg Gly Ser 250 255 260 atc caa tct gga aac tcc ggc ggc cca atg acc aac gaa atg ggt gaa 931 Ile Gln Ser Gly Asn Ser Gly Gly Pro Met Thr Asn Glu Met Gly Glu 265 270 275 gtg gtt ggt gtt gtc ttc ggc gca gcg atc gac ggc tcc gat acc ggt 979 Val Val Gly Val Val Phe Gly Ala Ala Ile Asp Gly Ser Asp Thr Gly 280 285 290 tac gtt ctc act gcc gaa gag gta cag gag cgg atc ggc gac atc acc 1027 Tyr Val Leu Thr Ala Glu Glu Val Gln Glu Arg Ile Gly Asp Ile Thr 295 300 305 gcg ctg act cag cct gtc gat acg atg cag tgc gcg gtt tct 1069 Ala Leu Thr Gln Pro Val Asp Thr Met Gln Cys Ala Val Ser 310 315 320 tagtcgtcgg gagctaggac cagacaggga 1099 <210> 158 <211> 323 <212> PRT <213> Corynebacterium glutamicum <400> 158 Leu Gly Asn Leu Ile Gly Ala His Leu Gly Ala Ala Ile Arg Asp Asn 1 5 10 15 Ile Lys Phe Arg Ser Ser Arg Thr Leu Asp Ser Gly Leu Gly Ala Ile 20 25 30 Phe Gln Val Leu Ala Thr Leu Ile Val Val Trp Leu Val Ala Ile Pro 35 40 45 Leu Ala Thr Gly Leu Pro Gly Thr Val Ala Ser Gly Ile Arg Asp Ser 50 55 60 Arg Ile Leu Gly Phe Val Asp Lys Tyr Thr Pro Gln Gly Leu Asp Thr 65 70 75 80 Leu Pro Ser Lys Ile Ala Ala Met Leu Ser Glu Ser Gly Leu Pro Pro 85 90 95 Leu Ile Ser Pro Phe Thr Gly Gly Ser Ser Val Glu Val Asp Ala Pro 100 105 110 Glu Ile Asn Val Thr Asn Val Asp Leu Val Glu Ala Met Arg Pro Ser 115 120 125 Val Ile His Val Met Gly Asp Ala Gln Glu Cys Ser Arg Arg Leu Met 130 135 140 Gly Ser Gly Phe Val Ala Ser Pro Asp Tyr Val Val Thr Asn Ala His 145 150 155 160 Val Val Ala Gly Thr Ser Thr Val Ser Leu Asp Thr Met Ile Gly Thr 165 170 175 Arg Ser Ala Glu Val Val Phe Tyr Asp Pro Asn Leu Asp Ile Ala Val 180 185 190 Leu Tyr Ser Pro Asp Leu Gly Leu Asp Pro Leu Pro Trp Ala Ser Thr 195 200 205 Pro Leu Asp Thr Gly Asp Glu Ala Ile Val Met Gly Phe Pro Gln Ser 210 215 220 Gly Pro Phe Asn Ala Ser Pro Ala Arg Val Arg Glu Arg Ile Met Ile 225 230 235 240 Thr Gly Ser Asn Ile Tyr Ala Asn Gly Gln His Glu Arg Glu Ala Tyr 245 250 255 Ser Val Arg Gly Ser Ile Gln Ser Gly Asn Ser Gly Gly Pro Met Thr 260 265 270 Asn Glu Met Gly Glu Val Val Gly Val Val Phe Gly Ala Ala Ile Asp 275 280 285 Gly Ser Asp Thr Gly Tyr Val Leu Thr Ala Glu Glu Val Gln Glu Arg 290 295 300 Ile Gly Asp Ile Thr Ala Leu Thr Gln Pro Val Asp Thr Met Gln Cys 305 310 315 320 Ala val ser <210> 159 <211> 747 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (1) .. (747) <223> RXA07014 <400> 159 gtg cgc ctc gca gct gcc act ggt ttc tcc ttt gtc ggt ctg cgc gtc 48 Val Arg Leu Ala Ala Ala Thr Gly Phe Ser Phe Val Gly Leu Arg Val 1 5 10 15 atc gca gta acc ccc aac gaa cgt gta tat gac ctt tcc cca gga tcc 96 Ile Ala Val Thr Pro Asn Glu Arg Val Tyr Asp Leu Ser Pro Gly Ser 20 25 30 cca ctg ctg gct gca acc caa caa gcg ttg aaa gaa acc gcc ctg tat 144 Pro Leu Leu Ala Ala Thr Gln Gln Ala Leu Lys Glu Thr Ala Leu Tyr 35 40 45 gtg ctc gac act gaa ttc cta cag gta aac gca gac acc acc cgc gag 192 Val Leu Asp Thr Glu Phe Leu Gln Val Asn Ala Asp Thr Thr Arg Glu 50 55 60 gcc tgg ctt ccc gca cta gaa gcc gcc gga gca ctg gga gct aaa acc 240 Ala Trp Leu Pro Ala Leu Glu Ala Ala Gly Ala Leu Gly Ala Lys Thr 65 70 75 80 ttc acc atc gcc gcc ggt gat gac aac att gcg ccc ctg acc gac acg 288 Phe Thr Ile Ala Ala Gly Asp Asp Asn Ile Ala Pro Leu Thr Asp Thr 85 90 95 atc ggt gcc atg gtt gac gat gcc cgt gat ttc gga gtc acc cca gcc 336 Ile Gly Ala Met Val Asp Asp Ala Arg Asp Phe Gly Val Thr Pro Ala 100 105 110 cta gag cca atc tct tac cgc agc gtg cat tcc att ccg cag gca gca 384 Leu Glu Pro Ile Ser Tyr Arg Ser Val His Ser Ile Pro Gln Ala Ala 115 120 125 gca atc gcc aga gac tcc ggc gga aaa gtc gtg gcg gac acc ttg cac 432 Ala Ile Ala Arg Asp Ser Gly Gly Lys Val Val Ala Asp Thr Leu His 130 135 140 atg gcc agg ttc gga gcc acc cca gaa gaa ctc acc gac gtc acc gat 480 Met Ala Arg Phe Gly Ala Thr Pro Glu Glu Leu Thr Asp Val Thr Asp 145 150 155 160 gtt cta gga gtt ctc cag ctt tgc gat agc cct ctg caa cgc ccg gct 528 Val Leu Gly Val Leu Gln Leu Cys Asp Ser Pro Leu Gln Arg Pro Ala 165 170 175 gac ctc gac gga ctg gtt aca gaa tcc cgc tcc ctg cgt ctg gct ccc 576 Asp Leu Asp Gly Leu Val Thr Glu Ser Arg Ser Leu Arg Leu Ala Pro 180 185 190 ggc gaa ggt gaa caa aac ctc ctg acc ttt gtc aac gcc ctg gat tct 624 Gly Glu Gly Glu Gln Asn Leu Leu Thr Phe Val Asn Ala Leu Asp Ser 195 200 205 aac gtc cca ctc agc gtg gag acc ccc aac gat ggg gaa gta gcc tct 672 Asn Val Pro Leu Ser Val Glu Thr Pro Asn Asp Gly Glu Val Ala Ser 210 215 220 cgt ggc gac gcc gga tgg atc aac cac ctc tac aac acc acc caa gct 720 Arg Gly Asp Ala Gly Trp Ile Asn His Leu Tyr Asn Thr Thr Gln Ala 225 230 235 240 ctg tta gaa aaa att gag gaa gca gtc 747 Leu Leu Glu Lys Ile Glu Glu Ala Val 245 <210> 160 <211> 249 <212> PRT <213> Corynebacterium glutamicum <400> 160 Val Arg Leu Ala Ala Ala Thr Gly Phe Ser Phe Val Gly Leu Arg Val 1 5 10 15 Ile Ala Val Thr Pro Asn Glu Arg Val Tyr Asp Leu Ser Pro Gly Ser 20 25 30 Pro Leu Leu Ala Ala Thr Gln Gln Ala Leu Lys Glu Thr Ala Leu Tyr 35 40 45 Val Leu Asp Thr Glu Phe Leu Gln Val Asn Ala Asp Thr Thr Arg Glu 50 55 60 Ala Trp Leu Pro Ala Leu Glu Ala Ala Gly Ala Leu Gly Ala Lys Thr 65 70 75 80 Phe Thr Ile Ala Ala Gly Asp Asp Asn Ile Ala Pro Leu Thr Asp Thr 85 90 95 Ile Gly Ala Met Val Asp Asp Ala Arg Asp Phe Gly Val Thr Pro Ala 100 105 110 Leu Glu Pro Ile Ser Tyr Arg Ser Val His Ser Ile Pro Gln Ala Ala 115 120 125 Ala Ile Ala Arg Asp Ser Gly Gly Lys Val Val Ala Asp Thr Leu His 130 135 140 Met Ala Arg Phe Gly Ala Thr Pro Glu Glu Leu Thr Asp Val Thr Asp 145 150 155 160 Val Leu Gly Val Leu Gln Leu Cys Asp Ser Pro Leu Gln Arg Pro Ala 165 170 175 Asp Leu Asp Gly Leu Val Thr Glu Ser Arg Ser Leu Arg Leu Ala Pro 180 185 190 Gly Glu Gly Glu Gln Asn Leu Leu Thr Phe Val Asn Ala Leu Asp Ser 195 200 205 Asn Val Pro Leu Ser Val Glu Thr Pro Asn Asp Gly Glu Val Ala Ser 210 215 220 Arg Gly Asp Ala Gly Trp Ile Asn His Leu Tyr Asn Thr Thr Gln Ala 225 230 235 240 Leu Leu Glu Lys Ile Glu Glu Ala Val 245 <210> 161 <211> 618 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (1) .. (618) <223> RXA07015 <400> 161 atg ggc caa tgc acg gta gcg ctt tac cga gag cat tcc tat tac aca 48 Met Gly Gln Cys Thr Val Ala Leu Tyr Arg Glu His Ser Tyr Tyr Thr 1 5 10 15 gca acc cca tgg cga gga acc tgg gca gcc gat ggc ggt gga gtg ctc 96 Ala Thr Pro Trp Arg Gly Thr Trp Ala Ala Asp Gly Gly Val Leu 20 25 30 atg act caa gcc atc cac tac atc gat ctt ttg tac tgg ctg ttg ggc 144 Met Thr Gln Ala Ile His Tyr Ile Asp Leu Leu Tyr Trp Leu Leu Gly 35 40 45 gaa ccc gtg gaa gtt ttc ggc tac acc aac tcc ttc aaa cac ggc gac 192 Glu Pro Val Glu Val Phe Gly Tyr Thr Asn Ser Phe Lys His Gly Asp 50 55 60 aac atc gaa gtc gaa gac agc gcc gtt gcc act gtg cgt ttt gaa tcg 240 Asn Ile Glu Val Glu Asp Ser Ala Val Ala Thr Val Arg Phe Glu Ser 65 70 75 80 ggc gcg ttg gcc aca att tca gcc acc acc gcc gca gag cca gca ctc 288 Gly Ala Leu Ala Thr Ile Ser Ala Thr Thr Ala Ala Glu Pro Ala Leu 85 90 95 ggc gca caa gtg cag gtg atg gga aca aag ggt gcc acc atg acg atc 336 Gly Ala Gln Val Gln Val Met Gly Thr Lys Gly Ala Thr Met Thr Ile 100 105 110 ctg gaa ttc cct gaa ggt acc gac ggc agg ctc att gtt cgc agt gaa 384 Leu Glu Phe Pro Glu Gly Thr Asp Gly Arg Leu Ile Val Arg Ser Glu 115 120 125 aac gac acc gtc gaa acc acc cca tcc cac ccc gcg gat ctt tat ccc 432 Asn Asp Thr Val Glu Thr Thr Pro Ser His Pro Ala Asp Leu Tyr Pro 130 135 140 aat gcc gat ctt tcc atc atc aac ggt gct ttg atc ccg tat cac acc 480 Asn Ala Asp Leu Ser Ile Ile Asn Gly Ala Leu Ile Pro Tyr His Thr 145 150 155 160 gcc cag atc gca gac ttt atc gat gcg ctc aac gaa ggc cgc cca cca 528 Ala Gln Ile Ala Asp Phe Ile Asp Ala Leu Asn Glu Gly Arg Pro Pro 165 170 175 ctg atc acc ggc cgc gat gcc acc aga gct ctg aaa gtt ctc ctt ggt 576 Leu Ile Thr Gly Arg Asp Ala Thr Arg Ala Leu Lys Val Leu Leu Gly 180 185 190 gtc tac gaa tca gca gcc acc cac cag ccg gtc tct ttg atc 618 Val Tyr Glu Ser Ala Ala Thr His Gln Pro Val Ser Leu Ile 195 200 205 <210> 162 <211> 206 <212> PRT <213> Corynebacterium glutamicum <400> 162 Met Gly Gln Cys Thr Val Ala Leu Tyr Arg Glu His Ser Tyr Tyr Thr 1 5 10 15 Ala Thr Pro Trp Arg Gly Thr Trp Ala Ala Asp Gly Gly Val Leu 20 25 30 Met Thr Gln Ala Ile His Tyr Ile Asp Leu Leu Tyr Trp Leu Leu Gly 35 40 45 Glu Pro Val Glu Val Phe Gly Tyr Thr Asn Ser Phe Lys His Gly Asp 50 55 60 Asn Ile Glu Val Glu Asp Ser Ala Val Ala Thr Val Arg Phe Glu Ser 65 70 75 80 Gly Ala Leu Ala Thr Ile Ser Ala Thr Thr Ala Ala Glu Pro Ala Leu 85 90 95 Gly Ala Gln Val Gln Val Met Gly Thr Lys Gly Ala Thr Met Thr Ile 100 105 110 Leu Glu Phe Pro Glu Gly Thr Asp Gly Arg Leu Ile Val Arg Ser Glu 115 120 125 Asn Asp Thr Val Glu Thr Thr Pro Ser His Pro Ala Asp Leu Tyr Pro 130 135 140 Asn Ala Asp Leu Ser Ile Ile Asn Gly Ala Leu Ile Pro Tyr His Thr 145 150 155 160 Ala Gln Ile Ala Asp Phe Ile Asp Ala Leu Asn Glu Gly Arg Pro Pro 165 170 175 Leu Ile Thr Gly Arg Asp Ala Thr Arg Ala Leu Lys Val Leu Leu Gly 180 185 190 Val Tyr Glu Ser Ala Ala Thr His Gln Pro Val Ser Leu Ile 195 200 205 <210> 163 <211> 993 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (1) .. (993) <223> RXA07016 <400> 163 atg aca gtt gca cac aaa cga agt ctg aca tgg atc aag aga cta tct 48 Met Thr Val Ala His Lys Arg Ser Leu Thr Trp Ile Lys Arg Leu Ser 1 5 10 15 gcg act aca ttt gca gct ttt ctt ggc att cag ctt gtg acc ccc gct 96 Ala Thr Thr Phe Ala Ala Phe Leu Gly Ile Gln Leu Val Thr Pro Ala 20 25 30 cac tca att gaa aac acg acg cag att cca gaa agc gaa ttg cac aac 144 His Ser Ile Glu Asn Thr Thr Gln Ile Pro Glu Ser Glu Leu His Asn 35 40 45 ctc ggg ctc aca gac gaa gaa att caa gaa ttc aat caa tat ctc att 192 Leu Gly Leu Thr Asp Glu Glu Ile Gln Glu Phe Asn Gln Tyr Leu Ile 50 55 60 gac gag tca ctc ttt caa gag act gta gaa acc tct ccg atc gta gtc 240 Asp Glu Ser Leu Phe Gln Glu Thr Val Glu Thr Ser Pro Ile Val Val 65 70 75 80 agc gac aat gaa gat gca gcc caa gat ccc ggt ttc ggc ctt ttc acc 288 Ser Asp Asn Glu Asp Ala Ala Gln Asp Pro Gly Phe Gly Leu Phe Thr 85 90 95 aca aat cct gtg aaa cac aca gat gag cac att ggc gct cta tac ttc 336 Thr Asn Pro Val Lys His Thr Asp Glu His Ile Gly Ala Leu Tyr Phe 100 105 110 tcc gac cta cca ggt att tcc aat ctt acg tgc act gcc aac tac atc 384 Ser Asp Leu Pro Gly Ile Ser Asn Leu Thr Cys Thr Ala Asn Tyr Ile 115 120 125 ggt gga aaa ttc tgg acc aca gca cat cat tgt gtt gaa ggc cgc agt 432 Gly Gly Lys Phe Trp Thr Thr Ala His His Cys Val Glu Gly Arg Ser 130 135 140 cgc ttc gtc ggc ttc atc gag cag tcg gac gga caa tat gca ggc att 480 Arg Phe Val Gly Phe Ile Glu Gln Ser Asp Gly Gln Tyr Ala Gly Ile 145 150 155 160 gag cat gtt tac aca aag tca agt gac tac gat att gcc ctc att aaa 528 Glu His Val Tyr Thr Lys Ser Ser Asp Tyr Asp Ile Ala Leu Ile Lys 165 170 175 gtg gga tct gga att gac gca gaa cag ttc agc ctc act cga tca cgc 576 Val Gly Ser Gly Ile Asp Ala Glu Gln Phe Ser Leu Thr Arg Ser Arg 180 185 190 cct gcg gtt gga aaa atg ctg gaa gtc gtt ggg ttt gca gtt gtg aac 624 Pro Ala Val Gly Lys Met Leu Glu Val Val Gly Phe Ala Val Val Asn 195 200 205 cca ttc tcc tct aga gca aca atg gaa gtc acc aac tca aac aca gtc 672 Pro Phe Ser Ser Arg Ala Thr Met Glu Val Thr Asn Ser Asn Thr Val 210 215 220 gcg aac tat cct atg agc aac ctg tac tac aaa gac gta ttc tcg gct 720 Ala Asn Tyr Pro Met Ser Asn Leu Tyr Tyr Lys Asp Val Phe Ser Ala 225 230 235 240 aaa ccc gca gga tca ttc cct tat gtc att tcc gga ggt gat tcc ggc 768 Lys Pro Ala Gly Ser Phe Pro Tyr Val Ile Ser Gly Gly Asp Ser Gly 245 250 255 gca gct gtt tgg tcg ggt gaa acc atg tac ggc att gct agt ggc tct 816 Ala Ala Val Trp Ser Gly Glu Thr Met Tyr Gly Ile Ala Ser Gly Ser 260 265 270 aac ggc agc act aaa aac cta gca gca aat gtt gga ccg cac gtc tct 864 Asn Gly Ser Thr Lys Asn Leu Ala Ala Asn Val Gly Pro His Val Ser 275 280 285 tgg atc gaa caa acg atg caa aac aat agc aat tcc tct ccc tat gaa 912 Trp Ile Glu Gln Thr Met Gln Asn Asn Ser Asn Ser Ser Pro Tyr Glu 290 295 300 att ttg caa gcg ttc cga ggc ggc ctt gca acg tct tac ccc aaa tat 960 Ile Leu Gln Ala Phe Arg Gly Gly Leu Ala Thr Ser Tyr Pro Lys Tyr 305 310 315 320 aat caa att aga gac cta gtc gac tca agt agt 993 Asn Gln Ile Arg Asp Leu Val Asp Ser Ser Ser 325 330 <210> 164 <211> 331 <212> PRT <213> Corynebacterium glutamicum <400> 164 Met Thr Val Ala His Lys Arg Ser Leu Thr Trp Ile Lys Arg Leu Ser 1 5 10 15 Ala Thr Thr Phe Ala Ala Phe Leu Gly Ile Gln Leu Val Thr Pro Ala 20 25 30 His Ser Ile Glu Asn Thr Thr Gln Ile Pro Glu Ser Glu Leu His Asn 35 40 45 Leu Gly Leu Thr Asp Glu Glu Ile Gln Glu Phe Asn Gln Tyr Leu Ile 50 55 60 Asp Glu Ser Leu Phe Gln Glu Thr Val Glu Thr Ser Pro Ile Val Val 65 70 75 80 Ser Asp Asn Glu Asp Ala Ala Gln Asp Pro Gly Phe Gly Leu Phe Thr 85 90 95 Thr Asn Pro Val Lys His Thr Asp Glu His Ile Gly Ala Leu Tyr Phe 100 105 110 Ser Asp Leu Pro Gly Ile Ser Asn Leu Thr Cys Thr Ala Asn Tyr Ile 115 120 125 Gly Gly Lys Phe Trp Thr Thr Ala His His Cys Val Glu Gly Arg Ser 130 135 140 Arg Phe Val Gly Phe Ile Glu Gln Ser Asp Gly Gln Tyr Ala Gly Ile 145 150 155 160 Glu His Val Tyr Thr Lys Ser Ser Asp Tyr Asp Ile Ala Leu Ile Lys 165 170 175 Val Gly Ser Gly Ile Asp Ala Glu Gln Phe Ser Leu Thr Arg Ser Arg 180 185 190 Pro Ala Val Gly Lys Met Leu Glu Val Val Gly Phe Ala Val Val Asn 195 200 205 Pro Phe Ser Ser Arg Ala Thr Met Glu Val Thr Asn Ser Asn Thr Val 210 215 220 Ala Asn Tyr Pro Met Ser Asn Leu Tyr Tyr Lys Asp Val Phe Ser Ala 225 230 235 240 Lys Pro Ala Gly Ser Phe Pro Tyr Val Ile Ser Gly Gly Asp Ser Gly 245 250 255 Ala Ala Val Trp Ser Gly Glu Thr Met Tyr Gly Ile Ala Ser Gly Ser 260 265 270 Asn Gly Ser Thr Lys Asn Leu Ala Ala Asn Val Gly Pro His Val Ser 275 280 285 Trp Ile Glu Gln Thr Met Gln Asn Asn Ser Asn Ser Ser Pro Tyr Glu 290 295 300 Ile Leu Gln Ala Phe Arg Gly Gly Leu Ala Thr Ser Tyr Pro Lys Tyr 305 310 315 320 Asn Gln Ile Arg Asp Leu Val Asp Ser Ser Ser 325 330 <210> 165 <211> 3885 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (1) .. (3885) <223> RXA07025 <400> 165 gtg gca ttg agc agt gtt cca gca cag ttc ctg aga tcc gcc cag gcg 48 Val Ala Leu Ser Ser Val Pro Ala Gln Phe Leu Arg Ser Ala Gln Ala 1 5 10 15 ccc ccg aag cgt act ttg tgg gac gtc tta gaa tcc gtc gcc tct act 96 Pro Pro Lys Arg Thr Leu Trp Asp Val Leu Glu Ser Val Ala Ser Thr 20 25 30 tat cct gag gca gca gct att gac gat ggc cag gtg ttg acc tac gca 144 Tyr Pro Glu Ala Ala Ala Ile Asp Asp Gly Gln Val Leu Thr Tyr Ala 35 40 45 gag ttg atg gaa gaa gtc acc gcg ttg gct gat tcc att cat gca cag 192 Glu Leu Met Glu Glu Val Thr Ala Leu Ala Asp Ser Ile His Ala Gln 50 55 60 ggc att cgc cgt ggt gat cgc atc ggt att cgc atg ccg tct ggt acg 240 Gly Ile Arg Arg Gly Asp Arg Ile Gly Ile Arg Met Pro Ser Gly Thr 65 70 75 80 cgt gac ctt tac atc gct att ttg gcc act ctc gct gct ggt gct gct 288 Arg Asp Leu Tyr Ile Ala Ile Leu Ala Thr Leu Ala Ala Gly Ala Ala 85 90 95 tac gtg cca gtt gat gca gat gat cct gaa gag cgc gcc gag atg gtg 336 Tyr Val Pro Val Asp Ala Asp Asp Pro Glu Glu Arg Ala Glu Met Val 100 105 110 ttt ggt gaa gca aat att aat gcg ctt ttc gac gcc acc ggc ttc cat 384 Phe Gly Glu Ala Asn Ile Asn Ala Leu Phe Asp Ala Thr Gly Phe His 115 120 125 atg ctt cgc ccg acc gcg ggc ggc gat acc cgt aga cca cgc ttg gat 432 Met Leu Arg Pro Thr Ala Gly Gly Asp Thr Arg Arg Pro Arg Leu Asp 130 135 140 gat acg gcg tgg att atc ttt act tcc ggt tcc acc ggc aag cct aag 480 Asp Thr Ala Trp Ile Ile Phe Thr Ser Gly Ser Thr Gly Lys Pro Lys 145 150 155 160 ggt gtg gct gtg tcc cac cgt tca gct gcg gct ttc gtg gat gcc gaa 528 Gly Val Ala Val Ser His Arg Ser Ala Ala Ala Phe Val Asp Ala Glu 165 170 175 gca caa atg ttc ctt gtc gat cac cct tcc ggc ccc ctt ggc cca gaa 576 Ala Gln Met Phe Leu Val Asp His Pro Ser Gly Pro Leu Gly Pro Glu 180 185 190 gac cga gtc ctt gcg gga ttg tct gta gcc ttt gac gca tct tgt gag 624 Asp Arg Val Leu Ala Gly Leu Ser Val Ala Phe Asp Ala Ser Cys Glu 195 200 205 gaa atg tgg ttg gcc tgg ggc cac ggc gcc tgc ttg gtg cca gca cca 672 Glu Met Trp Leu Ala Trp Gly His Gly Ala Cys Leu Val Pro Ala Pro 210 215 220 cgc tcc cta gtc cgt tcc ggt atg gac ttg ggc cca tgg ctg att cgc 720 Arg Ser Leu Val Arg Ser Gly Met Asp Leu Gly Pro Trp Leu Ile Arg 225 230 235 240 cgc gac atc agt gtc gtc tcc acc gtc cca act ctg gct ggt ctg tgg 768 Arg Asp Ile Ser Val Val Ser Thr Val Pro Thr Leu Ala Gly Leu Trp 245 250 255 cca gca gaa gca ttg tca cag gtc cgc ttg ctc atc gtc ggc ggc gag 816 Pro Ala Glu Ala Leu Ser Gln Val Arg Leu Leu Ile Val Gly Gly Glu 260 265 270 gct tgc tcg cag gag ctc gtt gaa cgc tta tcg acg cct gac cgc gag 864 Ala Cys Ser Gln Glu Leu Val Glu Arg Leu Ser Thr Pro Asp Arg Glu 275 280 285 gtg tgg aac act tac ggc ccc acc gaa gca acg gtg gtt gcc tgt ggc 912 Val Trp Asn Thr Tyr Gly Pro Thr Glu Ala Thr Val Val Ala Cys Gly 290 295 300 act caa ctc tat gct ggt cag cca gtg ggc att ggt ttg cca ctt gct 960 Thr Gln Leu Tyr Ala Gly Gln Pro Val Gly Ile Gly Leu Pro Leu Ala 305 310 315 320 ggt tgg gat ctt gtt gtt gtc gac gat gcc ggc gaa cct gtc gga atc 1008 Gly Trp Asp Leu Val Val Val Asp Asp Ala Gly Glu Pro Val Gly Ile 325 330 335 ggc gag gtc ggc gaa ttg gtc atc ggt ggt gtg ggt ctt gca cgc tac 1056 Gly Glu Val Gly Glu Leu Val Ile Gly Gly Val Gly Leu Ala Arg Tyr 340 345 350 ctt gat cca gaa aaa gac cgc gag aag tat gcg cca ctg aag tct gtt 1104 Leu Asp Pro Glu Lys Asp Arg Glu Lys Tyr Ala Pro Leu Lys Ser Val 355 360 365 ggt tgg acc cgc gct tat cgt tcc ggt gac cac gtt cgt ctg gaa gaa 1152 Gly Trp Thr Arg Ala Tyr Arg Ser Gly Asp His Val Arg Leu Glu Glu 370 375 380 gat ggc ctc tac ttt gtg ggc cgc gtt gat gat cag gtg aaa atc ggc 1200 Asp Gly Leu Tyr Phe Val Gly Arg Val Asp Asp Gln Val Lys Ile Gly 385 390 395 400 ggt cga cgc atc gag ctc ggt gaa gtt gat gcc aat gtg gca gcg ctt 1248 Gly Arg Arg Ile Glu Leu Gly Glu Val Asp Ala Asn Val Ala Ala Leu 405 410 415 tcc aac gtt cgt tcc tcc gca gtg gtt gtt cag acc act ggt gcg gat 1296 Ser Asn Val Arg Ser Ser Ala Val Val Val Gln Thr Thr Gly Ala Asp 420 425 430 caa aaa gtt ctg gtt gca tac gtt tct ttg gaa gat gct gca gct gga 1344 Gln Lys Val Leu Val Ala Tyr Val Ser Leu Glu Asp Ala Ala Ala Gly 435 440 445 ttt gat cac aac gtc gcg act gcc cga ctc acc gaa acc atg cct gct 1392 Phe Asp His Asn Val Ala Thr Ala Arg Leu Thr Glu Thr Met Pro Ala 450 455 460 gct ttg gtt ccg cgc att cac gtg atg gat gat ctg cct gtc acc acc 1440 Ala Leu Val Pro Arg Ile His Val Met Asp Asp Leu Pro Val Thr Thr 465 470 475 480 tcc ggc aag gtt gat aag aag tct ttg ccg tgg cct ctt cct ggc acc 1488 Ser Gly Lys Val Asp Lys Lys Ser Leu Pro Trp Pro Leu Pro Gly Thr 485 490 495 gtg gtg gaa gct aat gac ctc agc gca acg gaa gcc tgg att gct cag 1536 Val Val Glu Ala Asn Asp Leu Ser Ala Thr Glu Ala Trp Ile Ala Gln 500 505 510 gaa tgg gtc gat atc ctc ggc act tct gtg agc agc aaa gac gcc gac 1584 Glu Trp Val Asp Ile Leu Gly Thr Ser Val Ser Ser Lys Asp Ala Asp 515 520 525 ttc ttc tcc ctt ggc ggt acc tct ctc gcg gct gcg act ttg gtt ggc 1632 Phe Phe Ser Leu Gly Gly Thr Ser Leu Ala Ala Ala Thr Leu Val Gly 530 535 540 cgg gta cgt gca aag gtt ccc acc gct gcg gtg cgt gat ctt tac gat 1680 Arg Val Arg Ala Lys Val Pro Thr Ala Ala Val Arg Asp Leu Tyr Asp 545 550 555 560 cac cct cgc ttg gag aaa ttc gcc gag cgt gtc gag gct atc gcc gcc 1728 His Pro Arg Leu Glu Lys Phe Ala Glu Arg Val Glu Ala Ile Ala Ala 565 570 575 gac act ggc att tct ttg gag gcg cca aac cag gtg gag gag cgc gtc 1776 Asp Thr Gly Ile Ser Leu Glu Ala Pro Asn Gln Val Glu Glu Arg Val 580 585 590 gtc aag cct gtt tct ttt ggc act cgt gtg atg cag acc ctc atc cag 1824 Val Lys Pro Val Ser Phe Gly Thr Arg Val Met Gln Thr Leu Ile Gln 595 600 605 att ccg atc atg acg ctg caa gca gca cag tgg att gca tgg ttg ctg 1872 Ile Pro Ile Met Thr Leu Gln Ala Ala Gln Trp Ile Ala Trp Leu Leu 610 615 620 ttg ggc aac aac atc atg gca gcg ctt gat ttc gat tgg gct gtt cat 1920 Leu Gly Asn Asn Ile Met Ala Ala Leu Asp Phe Asp Trp Ala Val His 625 630 635 640 gtc tcc tgg tgg ctt gtc atc ggc atg att ttg gtg ttc gct acc ccg 1968 Val Ser Trp Trp Leu Val Ile Gly Met Ile Leu Val Phe Ala Thr Pro 645 650 655 att ggt cgc ttg ccg atc ggc ggt tgg ggc gcc cgc atc atc acc cgt 2016 Ile Gly Arg Leu Pro Ile Gly Gly Trp Gly Ala Arg Ile Ile Thr Arg 660 665 670 ggc ata act cct ggc tcc tac cct cgt ggc ggt tcc act cac ctg cgc 2064 Gly Ile Thr Pro Gly Ser Tyr Pro Arg Gly Gly Ser Thr His Leu Arg 675 680 685 att tgg tcc gcc gag cgc ctt gct gat gcc tct ggc tct cgc aat att 2112 Ile Trp Ser Ala Glu Arg Leu Ala Asp Ala Ser Gly Ser Arg Asn Ile 690 695 700 tct ggc gca acc tgg gtg aac tac ttc gcg cgt tcc ctg ggt gtg aag 2160 Ser Gly Ala Thr Trp Val Asn Tyr Phe Ala Arg Ser Leu Gly Val Lys 705 710 715 720 atg ggc aag ggc gtg gat ctt cac tcc ctg cca cca atc act ggc ctt 2208 Met Gly Lys Gly Val Asp Leu His Ser Leu Pro Pro Ile Thr Gly Leu 725 730 735 ttg acc ttg ggc aac aat gtt tcc atc gag caa gaa gtt gac ctt cgt 2256 Leu Thr Leu Gly Asn Asn Val Ser Ile Glu Gln Glu Val Asp Leu Arg 740 745 750 ggc tac tgg ctc gac ggc gat atc ctg cgt gta ggc acc att gag gtc 2304 Gly Tyr Trp Leu Asp Gly Asp Ile Leu Arg Val Gly Thr Ile Glu Val 755 760 765 cat gac aac gct cgt atc ggc gct cgt tcc acc ctg ctt ccc ggc acc 2352 His Asp Asn Ala Arg Ile Gly Ala Arg Ser Thr Leu Leu Pro Gly Thr 770 775 780 gtg gtg ggc acc ggc gct cac ctg ctg cct ggt tca aca gtg act ggt 2400 Val Val Gly Thr Gly Ala His Leu Leu Pro Gly Ser Thr Val Thr Gly 785 790 795 800 gat aag acc atc aag cct ggt tct cgt tgg gct ggc tcc cct gca caa 2448 Asp Lys Thr Ile Lys Pro Gly Ser Arg Trp Ala Gly Ser Pro Ala Gln 805 810 815 aag gtg ggt cgt gca aag cac cgc ttc cca acc tcc cat cct cca cgc 2496 Lys Val Gly Arg Ala Lys His Arg Phe Pro Thr Ser His Pro Pro Arg 820 825 830 agg tcc cgg tgg gtt ccg gtg ttc ggc gcg acc tcc atc gtg ttg tcg 2544 Arg Ser Arg Trp Val Pro Val Phe Gly Ala Thr Ser Ile Val Leu Ser 835 840 845 ctg ctg cca ctt cag gct ctc gct att ggc gct gct atc acc ttg tgg 2592 Leu Leu Pro Leu Gln Ala Leu Ala Ile Gly Ala Ala Ile Thr Leu Trp 850 855 860 ctg gcc acg att agc ccg ctt cca ctg atc tgg ggt gtg ctg gtt ttt 2640 Leu Ala Thr Ile Ser Pro Leu Pro Leu Ile Trp Gly Val Leu Val Phe 865 870 875 880 gct acc gtc ggc gcg ttg gct gcg ttc ttt gct tac acc gtg acc atc 2688 Ala Thr Val Gly Ala Leu Ala Ala Phe Phe Ala Tyr Thr Val Thr Ile 885 890 895 tgg gtg ctt gtc cgt ttg atc cag atc ggc atc aag ggc ggc acc gca 2736 Trp Val Leu Val Arg Leu Ile Gln Ile Gly Ile Lys Gly Gly Thr Ala 900 905 910 cca gtg agg tcc cgt ctt ggt tgg cag gtc tgg gca gtt caa cgc ctc 2784 Pro Val Arg Ser Arg Leu Gly Trp Gln Val Trp Ala Val Gln Arg Leu 915 920 925 atg gac gat gcc cgc acc tat ctc ttc ccg ctc tac gca tcc caa ctg 2832 Met Asp Asp Ala Arg Thr Tyr Leu Phe Pro Leu Tyr Ala Ser Gln Leu 930 935 940 acc cca ctg tgg ttc cgc agc ttg ggc gcg aag atc ggc aag gat gtt 2880 Thr Pro Leu Trp Phe Arg Ser Leu Gly Ala Lys Ile Gly Lys Asp Val 945 950 955 960 gag atc tcc acc gcg gtg atg gtt cct aaa ctg gct gat atc cgc gaa 2928 Glu Ile Ser Thr Ala Val Met Val Pro Lys Leu Ala Asp Ile Arg Glu 965 970 975 ggc gca ttc ctg gcc gat gac acc ctc atc ggt ggc tat gag ctg ggt 2976 Gly Ala Phe Leu Ala Asp Asp Thr Leu Ile Gly Gly Tyr Glu Leu Gly 980 985 990 aat ggt tgg ctg ctc agt ggt gaa acc cgc gtg ggt aag cgt tcc ttc 3024 Asn Gly Trp Leu Leu Ser Gly Glu Thr Arg Val Gly Lys Arg Ser Phe 995 1000 1005 att ggt aac tct ggc atc gca gga cct gag cgc aag ctc gct aag aac 3072 Ile Gly Asn Ser Gly Ile Ala Gly Pro Glu Arg Lys Leu Ala Lys Asn 1010 1015 1020 tcc ctg gtt gca gtg ctc tcc tcc acc ccg aag aag gct aag gcc aac 3120 Ser Leu Val Ala Val Leu Ser Ser Thr Pro Lys Lys Ala Lys Ala Asn 1025 1030 1035 1040 tcc aac tgg tgg ggt tcc cct cca gag cgc atg cgt cgt gtc act gtc 3168 Ser Asn Trp Trp Gly Ser Pro Pro Glu Arg Met Arg Arg Val Thr Val 1045 1050 1055 gaa gtt gat gag ggc gaa gca aag acc tac agc cct ggc ttt ggt gtg 3216 Glu Val Asp Glu Gly Glu Ala Lys Thr Tyr Ser Pro Gly Phe Gly Val 1060 1065 1070 aag ttt gca cgt ggc gcg gtg gaa acc gca cgt ctg ctt gct cca ata 3264 Lys Phe Ala Arg Gly Ala Val Glu Thr Ala Arg Leu Leu Ala Pro Ile 1075 1080 1085 acc tct ggt gtg ttg gct gcg ctg tca ctg ctg ctc atg cag tac ctg 3312 Thr Ser Gly Val Leu Ala Ala Leu Ser Leu Leu Leu Met Gln Tyr Leu 1090 1095 1100 ctc act gag ttc aac atg tgg atc acc tgg ttg ctt ggc gga ctg atc 3360 Leu Thr Glu Phe Asn Met Trp Ile Thr Trp Leu Leu Gly Gly Leu Ile 1105 1110 1115 1120 ctc atg acg gtt ggt gtg ctc gcc atg ggc att acg gtt gtg atg aag 3408 Leu Met Thr Val Gly Val Leu Ala Met Gly Ile Thr Val Val Met Lys 1125 1130 1135 tgg gtt tgc gtc ggc aag cat aag ccg tct gag cac cct ctc ttc agc 3456 Trp Val Cys Val Gly Lys His Lys Pro Ser Glu His Pro Leu Phe Ser 1140 1145 1150 cgc ttt gtg tgg ctg aat gag ctg caa gat gcg ttc gtg gaa tcc gtg 3504 Arg Phe Val Trp Leu Asn Glu Leu Gln Asp Ala Phe Val Glu Ser Val 1155 1160 1165 gct ggc cca tgg ttc ctc gtg ccc aac ctg ggc acc ggc gcg ctg aac 3552 Ala Gly Pro Trp Phe Leu Val Pro Asn Leu Gly Thr Gly Ala Leu Asn 1170 1175 1180 gcc ggc atg agc gcg ctt ggc gca cac atc ggc cgt ggc gca tgg atc 3600 Ala Gly Met Ser Ala Leu Gly Ala His Ile Gly Arg Gly Ala Trp Ile 1185 1190 1195 1200 gaa tcc tac tgg ctg ccg gaa acc gac ctc tgc tac atc ggc aag ggc 3648 Glu Ser Tyr Trp Leu Pro Glu Thr Asp Leu Cys Tyr Ile Gly Lys Gly 1205 1210 1215 gca acc gtg ggc cct ggc gtg gtc gtg cag acc cac ctc ttc cag gac 3696 Ala Thr Val Gly Pro Gly Val Val Val Gln Thr His Leu Phe Gln Asp 1220 1225 1230 cgc gtg atg agc cta gat acg gtg acc gtc gct gac ggc gcc acc cta 3744 Arg Val Met Ser Leu Asp Thr Val Thr Val Ala Asp Gly Ala Thr Leu 1235 1240 1245 gcg gac cac tcc gtt gcc ctt cct gct tcg ctt atc gac gcc tcc gcc 3792 Ala Asp His Ser Val Ala Leu Pro Ala Ser Leu Ile Asp Ala Ser Ala 1250 1255 1260 acc atc ggc cca ggc tcg ctg gtg atg cgc ggc gac aag gta cca gcg 3840 Thr Ile Gly Pro Gly Ser Leu Val Met Arg Gly Asp Lys Val Pro Ala 1265 1270 1275 1280 cat acc cgc tgg caa ggc aac cca att gag ccg tgg agc aac tct 3885 His Thr Arg Trp Gln Gly Asn Pro Ile Glu Pro Trp Ser Asn Ser 1285 1290 1295 <210> 166 <211> 1295 <212> PRT <213> Corynebacterium glutamicum <400> 166 Val Ala Leu Ser Ser Val Pro Ala Gln Phe Leu Arg Ser Ala Gln Ala 1 5 10 15 Pro Pro Lys Arg Thr Leu Trp Asp Val Leu Glu Ser Val Ala Ser Thr 20 25 30 Tyr Pro Glu Ala Ala Ala Ile Asp Asp Gly Gln Val Leu Thr Tyr Ala 35 40 45 Glu Leu Met Glu Glu Val Thr Ala Leu Ala Asp Ser Ile His Ala Gln 50 55 60 Gly Ile Arg Arg Gly Asp Arg Ile Gly Ile Arg Met Pro Ser Gly Thr 65 70 75 80 Arg Asp Leu Tyr Ile Ala Ile Leu Ala Thr Leu Ala Ala Gly Ala Ala 85 90 95 Tyr Val Pro Val Asp Ala Asp Asp Pro Glu Glu Arg Ala Glu Met Val 100 105 110 Phe Gly Glu Ala Asn Ile Asn Ala Leu Phe Asp Ala Thr Gly Phe His 115 120 125 Met Leu Arg Pro Thr Ala Gly Gly Asp Thr Arg Arg Pro Arg Leu Asp 130 135 140 Asp Thr Ala Trp Ile Ile Phe Thr Ser Gly Ser Thr Gly Lys Pro Lys 145 150 155 160 Gly Val Ala Val Ser His Arg Ser Ala Ala Ala Phe Val Asp Ala Glu 165 170 175 Ala Gln Met Phe Leu Val Asp His Pro Ser Gly Pro Leu Gly Pro Glu 180 185 190 Asp Arg Val Leu Ala Gly Leu Ser Val Ala Phe Asp Ala Ser Cys Glu 195 200 205 Glu Met Trp Leu Ala Trp Gly His Gly Ala Cys Leu Val Pro Ala Pro 210 215 220 Arg Ser Leu Val Arg Ser Gly Met Asp Leu Gly Pro Trp Leu Ile Arg 225 230 235 240 Arg Asp Ile Ser Val Val Ser Thr Val Pro Thr Leu Ala Gly Leu Trp 245 250 255 Pro Ala Glu Ala Leu Ser Gln Val Arg Leu Leu Ile Val Gly Gly Glu 260 265 270 Ala Cys Ser Gln Glu Leu Val Glu Arg Leu Ser Thr Pro Asp Arg Glu 275 280 285 Val Trp Asn Thr Tyr Gly Pro Thr Glu Ala Thr Val Val Ala Cys Gly 290 295 300 Thr Gln Leu Tyr Ala Gly Gln Pro Val Gly Ile Gly Leu Pro Leu Ala 305 310 315 320 Gly Trp Asp Leu Val Val Val Asp Asp Ala Gly Glu Pro Val Gly Ile 325 330 335 Gly Glu Val Gly Glu Leu Val Ile Gly Gly Val Gly Leu Ala Arg Tyr 340 345 350 Leu Asp Pro Glu Lys Asp Arg Glu Lys Tyr Ala Pro Leu Lys Ser Val 355 360 365 Gly Trp Thr Arg Ala Tyr Arg Ser Gly Asp His Val Arg Leu Glu Glu 370 375 380 Asp Gly Leu Tyr Phe Val Gly Arg Val Asp Asp Gln Val Lys Ile Gly 385 390 395 400 Gly Arg Arg Ile Glu Leu Gly Glu Val Asp Ala Asn Val Ala Ala Leu 405 410 415 Ser Asn Val Arg Ser Ser Ala Val Val Val Gln Thr Thr Gly Ala Asp 420 425 430 Gln Lys Val Leu Val Ala Tyr Val Ser Leu Glu Asp Ala Ala Ala Gly 435 440 445 Phe Asp His Asn Val Ala Thr Ala Arg Leu Thr Glu Thr Met Pro Ala 450 455 460 Ala Leu Val Pro Arg Ile His Val Met Asp Asp Leu Pro Val Thr Thr 465 470 475 480 Ser Gly Lys Val Asp Lys Lys Ser Leu Pro Trp Pro Leu Pro Gly Thr 485 490 495 Val Val Glu Ala Asn Asp Leu Ser Ala Thr Glu Ala Trp Ile Ala Gln 500 505 510 Glu Trp Val Asp Ile Leu Gly Thr Ser Val Ser Ser Lys Asp Ala Asp 515 520 525 Phe Phe Ser Leu Gly Gly Thr Ser Leu Ala Ala Ala Thr Leu Val Gly 530 535 540 Arg Val Arg Ala Lys Val Pro Thr Ala Ala Val Arg Asp Leu Tyr Asp 545 550 555 560 His Pro Arg Leu Glu Lys Phe Ala Glu Arg Val Glu Ala Ile Ala Ala 565 570 575 Asp Thr Gly Ile Ser Leu Glu Ala Pro Asn Gln Val Glu Glu Arg Val 580 585 590 Val Lys Pro Val Ser Phe Gly Thr Arg Val Met Gln Thr Leu Ile Gln 595 600 605 Ile Pro Ile Met Thr Leu Gln Ala Ala Gln Trp Ile Ala Trp Leu Leu 610 615 620 Leu Gly Asn Asn Ile Met Ala Ala Leu Asp Phe Asp Trp Ala Val His 625 630 635 640 Val Ser Trp Trp Leu Val Ile Gly Met Ile Leu Val Phe Ala Thr Pro 645 650 655 Ile Gly Arg Leu Pro Ile Gly Gly Trp Gly Ala Arg Ile Ile Thr Arg 660 665 670 Gly Ile Thr Pro Gly Ser Tyr Pro Arg Gly Gly Ser Thr His Leu Arg 675 680 685 Ile Trp Ser Ala Glu Arg Leu Ala Asp Ala Ser Gly Ser Arg Asn Ile 690 695 700 Ser Gly Ala Thr Trp Val Asn Tyr Phe Ala Arg Ser Leu Gly Val Lys 705 710 715 720 Met Gly Lys Gly Val Asp Leu His Ser Leu Pro Pro Ile Thr Gly Leu 725 730 735 Leu Thr Leu Gly Asn Asn Val Ser Ile Glu Gln Glu Val Asp Leu Arg 740 745 750 Gly Tyr Trp Leu Asp Gly Asp Ile Leu Arg Val Gly Thr Ile Glu Val 755 760 765 His Asp Asn Ala Arg Ile Gly Ala Arg Ser Thr Leu Leu Pro Gly Thr 770 775 780 Val Val Gly Thr Gly Ala His Leu Leu Pro Gly Ser Thr Val Thr Gly 785 790 795 800 Asp Lys Thr Ile Lys Pro Gly Ser Arg Trp Ala Gly Ser Pro Ala Gln 805 810 815 Lys Val Gly Arg Ala Lys His Arg Phe Pro Thr Ser His Pro Pro Arg 820 825 830 Arg Ser Arg Trp Val Pro Val Phe Gly Ala Thr Ser Ile Val Leu Ser 835 840 845 Leu Leu Pro Leu Gln Ala Leu Ala Ile Gly Ala Ala Ile Thr Leu Trp 850 855 860 Leu Ala Thr Ile Ser Pro Leu Pro Leu Ile Trp Gly Val Leu Val Phe 865 870 875 880 Ala Thr Val Gly Ala Leu Ala Ala Phe Phe Ala Tyr Thr Val Thr Ile 885 890 895 Trp Val Leu Val Arg Leu Ile Gln Ile Gly Ile Lys Gly Gly Thr Ala 900 905 910 Pro Val Arg Ser Arg Leu Gly Trp Gln Val Trp Ala Val Gln Arg Leu 915 920 925 Met Asp Asp Ala Arg Thr Tyr Leu Phe Pro Leu Tyr Ala Ser Gln Leu 930 935 940 Thr Pro Leu Trp Phe Arg Ser Leu Gly Ala Lys Ile Gly Lys Asp Val 945 950 955 960 Glu Ile Ser Thr Ala Val Met Val Pro Lys Leu Ala Asp Ile Arg Glu 965 970 975 Gly Ala Phe Leu Ala Asp Asp Thr Leu Ile Gly Gly Tyr Glu Leu Gly 980 985 990 Asn Gly Trp Leu Leu Ser Gly Glu Thr Arg Val Gly Lys Arg Ser Phe 995 1000 1005 Ile Gly Asn Ser Gly Ile Ala Gly Pro Glu Arg Lys Leu Ala Lys Asn 1010 1015 1020 Ser Leu Val Ala Val Leu Ser Ser Thr Pro Lys Lys Ala Lys Ala Asn 1025 1030 1035 1040 Ser Asn Trp Trp Gly Ser Pro Pro Glu Arg Met Arg Arg Val Thr Val 1045 1050 1055 Glu Val Asp Glu Gly Glu Ala Lys Thr Tyr Ser Pro Gly Phe Gly Val 1060 1065 1070 Lys Phe Ala Arg Gly Ala Val Glu Thr Ala Arg Leu Leu Ala Pro Ile 1075 1080 1085 Thr Ser Gly Val Leu Ala Ala Leu Ser Leu Leu Leu Met Gln Tyr Leu 1090 1095 1100 Leu Thr Glu Phe Asn Met Trp Ile Thr Trp Leu Leu Gly Gly Leu Ile 1105 1110 1115 1120 Leu Met Thr Val Gly Val Leu Ala Met Gly Ile Thr Val Val Met Lys 1125 1130 1135 Trp Val Cys Val Gly Lys His Lys Pro Ser Glu His Pro Leu Phe Ser 1140 1145 1150 Arg Phe Val Trp Leu Asn Glu Leu Gln Asp Ala Phe Val Glu Ser Val 1155 1160 1165 Ala Gly Pro Trp Phe Leu Val Pro Asn Leu Gly Thr Gly Ala Leu Asn 1170 1175 1180 Ala Gly Met Ser Ala Leu Gly Ala His Ile Gly Arg Gly Ala Trp Ile 1185 1190 1195 1200 Glu Ser Tyr Trp Leu Pro Glu Thr Asp Leu Cys Tyr Ile Gly Lys Gly 1205 1210 1215 Ala Thr Val Gly Pro Gly Val Val Val Gln Thr His Leu Phe Gln Asp 1220 1225 1230 Arg Val Met Ser Leu Asp Thr Val Thr Val Ala Asp Gly Ala Thr Leu 1235 1240 1245 Ala Asp His Ser Val Ala Leu Pro Ala Ser Leu Ile Asp Ala Ser Ala 1250 1255 1260 Thr Ile Gly Pro Gly Ser Leu Val Met Arg Gly Asp Lys Val Pro Ala 1265 1270 1275 1280 His Thr Arg Trp Gln Gly Asn Pro Ile Glu Pro Trp Ser Asn Ser 1285 1290 1295 <210> 167 <211> 2385 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS (222) (1) .. (2385) <223> RXA07026 <400> 167 gtg tcc acc acg aat tct ctg aca aag ctc gtt gca tct aca gtc gcc 48 Val Ser Thr Thr Asn Ser Leu Thr Lys Leu Val Ala Ser Thr Val Ala 1 5 10 15 gct ggc gtc ctt ggt gcg ctc gca ctt gtg cct ttc gct agt ctt tct 96 Ala Gly Val Leu Gly Ala Leu Ala Leu Val Pro Phe Ala Ser Leu Ser 20 25 30 ggc gtt gcg gtt gcg cgt acc aat gac acg atg cag acc aac ctt tca 144 Gly Val Ala Val Ala Arg Thr Asn Asp Thr Met Gln Thr Asn Leu Ser 35 40 45 gat ctg acg gat ggt cgc ggg ccg ggc gtc acg acg att act gat tcc 192 Asp Leu Thr Asp Gly Arg Gly Pro Gly Val Thr Thr Ile Thr Asp Ser 50 55 60 act gac cag ccg att gct tat att tat gcg cag cgg cgg ttt gag gtt 240 Thr Asp Gln Pro Ile Ala Tyr Ile Tyr Ala Gln Arg Arg Phe Glu Val 65 70 75 80 ggg ggt gat cag att tct acg tcg atg aag gat gcg atc gtt tcg att 288 Gly Gly Asp Gln Ile Ser Thr Ser Met Lys Asp Ala Ile Val Ser Ile 85 90 95 gag gat cgc agg ttc tat gag cat gat ggt gtg gat ttg cag ggc ttt 336 Glu Asp Arg Arg Phe Tyr Glu His Asp Gly Val Asp Leu Gln Gly Phe 100 105 110 ggt cgt gca atc ctg acg aac ctg gct gcg ggt ggc gtg gag cag ggt 384 Gly Arg Ala Ile Leu Thr Asn Leu Ala Ala Gly Gly Val Glu Gln Gly 115 120 125 gct tcg acg att aac cag cag tat gtg aag aac ttc ttg ctg ttg gtg 432 Ala Ser Thr Ile Asn Gln Gln Tyr Val Lys Asn Phe Leu Leu Leu Val 130 135 140 gaa gct gat gat gag gcg gag cag gct gct gct gtg gaa acc tcc atc 480 Glu Ala Asp Asp Glu Ala Glu Gln Ala Ala Ala Val Glu Thr Ser Ile 145 150 155 160 cct cgt aag ctc cgt gag atg aag atg gcg tct gat ttg gaa aag acg 528 Pro Arg Lys Leu Arg Glu Met Lys Met Ala Ser Asp Leu Glu Lys Thr 165 170 175 ttg tcg aag gat gag att ctg act cgt tat ctc aac att gtt cct ttt 576 Leu Ser Lys Asp Glu Ile Leu Thr Arg Tyr Leu Asn Ile Val Pro Phe 180 185 190 ggt aat ggt gct tat ggt gtt gag gct gcg gcg cgg acg tat ttc ggt 624 Gly Asn Gly Ala Tyr Gly Val Glu Ala Ala Ala Arg Thr Tyr Phe Gly 195 200 205 acg tcg gct gcc gag tta acc att cca cag tct gcg atg ctc gcg ggc 672 Thr Ser Ala Ala Glu Leu Thr Ile Pro Gln Ser Ala Met Leu Ala Gly 210 215 220 att gtg cag tct tcg tct tat ctc aat cca tac acc aat cac gat gct 720 Ile Val Gln Ser Ser Ser Tyr Leu Asn Pro Tyr Thr Asn His Asp Ala 225 230 235 240 gtg ttt gag cgt cgt aat act gtt ttg ggc gct atg gct gat gct ggc 768 Val Phe Glu Arg Arg Asn Thr Val Leu Gly Ala Met Ala Asp Ala Gly 245 250 255 gcg att tcc cca gac gag gct tcg gct ttc cag cag gaa cct ttg ggt 816 Ala Ile Ser Pro Asp Glu Ala Ser Ala Phe Gln Gln Glu Pro Leu Gly 260 265 270 gtc ctg gaa acc ccg caa ggc tta tcc aat ggt tgt atc ggc gct ggc 864 Val Leu Glu Thr Pro Gln Gly Leu Ser Asn Gly Cys Ile Gly Ala Gly 275 280 285 gat cgt ggt ttc ttc tgc gat tac gct ctg caa tat ctt tct gag cag 912 Asp Arg Gly Phe Phe Cys Asp Tyr Ala Leu Gln Tyr Leu Ser Glu Gln 290 295 300 gga atc acc caa gat atg ctg gcg aag gac tcc tac acc atc aaa ttg 960 Gly Ile Thr Gln Asp Met Leu Ala Lys Asp Ser Tyr Thr Ile Lys Leu 305 310 315 320 act ttg gat cca gat gtt cag gat gca gcg cac aat gcg gtg tcc tcc 1008 Thr Leu Asp Pro Asp Val Gln Asp Ala Ala His Asn Ala Val Ser Ser 325 330 335 cac gtt gat cca acc acc cca ggt gtc gct gaa gtt gtg aac gtc att 1056 His Val Asp Pro Thr Thr Pro Gly Val Ala Glu Val Val Asn Val Ile 340 345 350 gag cct ggc gag aac tcc cgc gat att ttg gct att act tct tcc cgc 1104 Glu Pro Gly Glu Asn Ser Arg Asp Ile Leu Ala Ile Thr Ser Ser Arg 355 360 365 aac tac ggc ctt gac ctg gat gct ggt gaa acg atg ctg cct cag gca 1152 Asn Tyr Gly Leu Asp Leu Asp Ala Gly Glu Thr Met Leu Pro Gln Ala 370 375 380 acg tcc cgt gtg ggt aat ggt gcc ggt tcc att ttc aag atc ttt acc 1200 Thr Ser Arg Val Gly Asn Gly Ala Gly Ser Ile Phe Lys Ile Phe Thr 385 390 395 400 gcc gct gca gcc att cag cag ggc gct ggc cta gac acc atg ttg gat 1248 Ala Ala Ala Ala Ile Gln Gln Gly Ala Gly Leu Asp Thr Met Leu Asp 405 410 415 gtt cct tct cga tat gag gtc aag ggc atg ggc tcc ggc ggt gcc gcg 1296 Val Pro Ser Arg Tyr Glu Val Lys Gly Met Gly Ser Gly Gly Ala Ala 420 425 430 aac tgt ccc gca aat act tac tgc gtg gaa aac gca gga tcc tac gcg 1344 Asn Cys Pro Ala Asn Thr Tyr Cys Val Glu Asn Ala Gly Ser Tyr Ala 435 440 445 cct cgc atg act ctg cag gac gct ctc gcg cag tcc ccc aac act gca 1392 Pro Arg Met Thr Leu Gln Asp Ala Leu Ala Gln Ser Pro Asn Thr Ala 450 455 460 ttc gtt gaa atg atc gag cag gtt ggc gtg gac acc gtt gtg gat ctt 1440 Phe Val Glu Met Ile Glu Gln Val Gly Val Asp Thr Val Val Asp Leu 465 470 475 480 tca gta aag ctg ggc ctg cga agc tac acc gat gaa ggt tcc ttc gac 1488 Ser Val Lys Leu Gly Leu Arg Ser Tyr Thr Asp Glu Gly Ser Phe Asp 485 490 495 ggc gaa agc tca atc gcg gac tac atg aag gac aac aac ctc ggt tct 1536 Gly Glu Ser Ser Ile Ala Asp Tyr Met Lys Asp Asn Asn Leu Gly Ser 500 505 510 tac act ctt gga cct acc gct gtt aac cct ctt gaa ttg tcc aat gtt 1584 Tyr Thr Leu Gly Pro Thr Ala Val Asn Pro Leu Glu Leu Ser Asn Val 515 520 525 gct gca acc att gca tcc ggt ggc atg tgg tgc gaa ccc aat ccc atc 1632 Ala Ala Thr Ile Ala Ser Gly Gly Met Trp Cys Glu Pro Asn Pro Ile 530 535 540 gcc agc gtc cat gac cgt gaa ggc aac gaa gtc tac att gac cgc cct 1680 Ala Ser Val His Asp Arg Glu Gly Asn Glu Val Tyr Ile Asp Arg Pro 545 550 555 560 gca tgt gag cgc gcc atc gat gcc gaa acg gct tca gct ttg gcc gtc 1728 Ala Cys Glu Arg Ala Ile Asp Ala Glu Thr Ala Ser Ala Leu Ala Val 565 570 575 ggc atg agc aag gat acg gtc agc gga act gcg gcc tct gca gcc agc 1776 Gly Met Ser Lys Asp Thr Val Ser Gly Thr Ala Ala Ser Ala Ala Ser 580 585 590 atg tac gga tgg tcc ttg cca acc gca gcg aag acc ggt acc acc gag 1824 Met Tyr Gly Trp Ser Leu Pro Thr Ala Ala Lys Thr Gly Thr Thr Glu 595 600 605 tcc aac cag tcc tca gca ttt atg ggc ttc aac agc aac ttt gcc gca 1872 Ser Asn Gln Ser Ser Ala Phe Met Gly Phe Asn Ser Asn Phe Ala Ala 610 615 620 gct cca tac atc tac aat gac ggc acc tcc acc acc cca ctg tgc agc 1920 Ala Pro Tyr Ile Tyr Asn Asp Gly Thr Ser Thr Thr Pro Leu Cys Ser 625 630 635 640 ggc ccc gtc cgc cag tgc agc agc ggt aac ctc ttc ggc ggt aac gaa 1968 Gly Pro Val Arg Gln Cys Ser Ser Gly Asn Leu Phe Gly Gly Asn Glu 645 650 655 cca gct caa aca tgg ttt aac atg gca agc aac gtc ccc gca gct tcg 2016 Pro Ala Gln Thr Trp Phe Asn Met Ala Ser Asn Val Pro Ala Ala Ser 660 665 670 caa gga aca ctg cca tcc agc agc gat tca ttc cgc ctc ggc act tcc 2064 Gln Gly Thr Leu Pro Ser Ser Ser Asp Ser Phe Arg Leu Gly Thr Ser 675 680 685 ggc gaa ctc ctc aac cag gtt gtc ggc caa agc gaa gcc tcc gct cga 2112 Gly Glu Leu Leu Asn Gln Val Val Gly Gln Ser Glu Ala Ser Ala Arg 690 695 700 cgc acc ctc gaa gcc aaa ggc tac aag gtc acc acg cgt tca gtc tcc 2160 Arg Thr Leu Glu Ala Lys Gly Tyr Lys Val Thr Thr Arg Ser Val Ser 705 710 715 720 ggc gcc ggc agc gcg cgc ggc acc gta gtc agc gca acc cct cag ggt 2208 Gly Ala Gly Ser Ala Arg Gly Thr Val Val Ser Ala Thr Pro Gln Gly 725 730 735 gca gtg ctt atc gac ggt gga acc gtc att ttg gac atc tcc gac ggc 2256 Ala Val Leu Ile Asp Gly Gly Thr Val Ile Leu Asp Ile Ser Asp Gly 740 745 750 aca agc cct gcc ccc gct gcc acc aac aat gat gac agc gac gat gga 2304 Thr Ser Pro Ala Pro Ala Ala Thr Asn Asn Asp Asp Ser Asp Asp Gly 755 760 765 gac acc cct gct cca tca aca aac aac cgc gga aca acc att gaa gac 2352 Asp Thr Pro Ala Pro Ser Thr Asn Asn Arg Gly Thr Thr Ile Glu Asp 770 775 780 gcc atc aat gac gcc atc aac cag ttc ttc cgc 2385 Ala Ile Asn Asp Ala Ile Asn Gln Phe Phe Arg 785 790 795 <210> 168 <211> 795 <212> PRT <213> Corynebacterium glutamicum <400> 168 Val Ser Thr Thr Asn Ser Leu Thr Lys Leu Val Ala Ser Thr Val Ala 1 5 10 15 Ala Gly Val Leu Gly Ala Leu Ala Leu Val Pro Phe Ala Ser Leu Ser 20 25 30 Gly Val Ala Val Ala Arg Thr Asn Asp Thr Met Gln Thr Asn Leu Ser 35 40 45 Asp Leu Thr Asp Gly Arg Gly Pro Gly Val Thr Thr Ile Thr Asp Ser 50 55 60 Thr Asp Gln Pro Ile Ala Tyr Ile Tyr Ala Gln Arg Arg Phe Glu Val 65 70 75 80 Gly Gly Asp Gln Ile Ser Thr Ser Met Lys Asp Ala Ile Val Ser Ile 85 90 95 Glu Asp Arg Arg Phe Tyr Glu His Asp Gly Val Asp Leu Gln Gly Phe 100 105 110 Gly Arg Ala Ile Leu Thr Asn Leu Ala Ala Gly Gly Val Glu Gln Gly 115 120 125 Ala Ser Thr Ile Asn Gln Gln Tyr Val Lys Asn Phe Leu Leu Leu Val 130 135 140 Glu Ala Asp Asp Glu Ala Glu Gln Ala Ala Ala Val Glu Thr Ser Ile 145 150 155 160 Pro Arg Lys Leu Arg Glu Met Lys Met Ala Ser Asp Leu Glu Lys Thr 165 170 175 Leu Ser Lys Asp Glu Ile Leu Thr Arg Tyr Leu Asn Ile Val Pro Phe 180 185 190 Gly Asn Gly Ala Tyr Gly Val Glu Ala Ala Ala Arg Thr Tyr Phe Gly 195 200 205 Thr Ser Ala Ala Glu Leu Thr Ile Pro Gln Ser Ala Met Leu Ala Gly 210 215 220 Ile Val Gln Ser Ser Ser Tyr Leu Asn Pro Tyr Thr Asn His Asp Ala 225 230 235 240 Val Phe Glu Arg Arg Asn Thr Val Leu Gly Ala Met Ala Asp Ala Gly 245 250 255 Ala Ile Ser Pro Asp Glu Ala Ser Ala Phe Gln Gln Glu Pro Leu Gly 260 265 270 Val Leu Glu Thr Pro Gln Gly Leu Ser Asn Gly Cys Ile Gly Ala Gly 275 280 285 Asp Arg Gly Phe Phe Cys Asp Tyr Ala Leu Gln Tyr Leu Ser Glu Gln 290 295 300 Gly Ile Thr Gln Asp Met Leu Ala Lys Asp Ser Tyr Thr Ile Lys Leu 305 310 315 320 Thr Leu Asp Pro Asp Val Gln Asp Ala Ala His Asn Ala Val Ser Ser 325 330 335 His Val Asp Pro Thr Thr Pro Gly Val Ala Glu Val Val Asn Val Ile 340 345 350 Glu Pro Gly Glu Asn Ser Arg Asp Ile Leu Ala Ile Thr Ser Ser Arg 355 360 365 Asn Tyr Gly Leu Asp Leu Asp Ala Gly Glu Thr Met Leu Pro Gln Ala 370 375 380 Thr Ser Arg Val Gly Asn Gly Ala Gly Ser Ile Phe Lys Ile Phe Thr 385 390 395 400 Ala Ala Ala Ala Ile Gln Gln Gly Ala Gly Leu Asp Thr Met Leu Asp 405 410 415 Val Pro Ser Arg Tyr Glu Val Lys Gly Met Gly Ser Gly Gly Ala Ala 420 425 430 Asn Cys Pro Ala Asn Thr Tyr Cys Val Glu Asn Ala Gly Ser Tyr Ala 435 440 445 Pro Arg Met Thr Leu Gln Asp Ala Leu Ala Gln Ser Pro Asn Thr Ala 450 455 460 Phe Val Glu Met Ile Glu Gln Val Gly Val Asp Thr Val Val Asp Leu 465 470 475 480 Ser Val Lys Leu Gly Leu Arg Ser Tyr Thr Asp Glu Gly Ser Phe Asp 485 490 495 Gly Glu Ser Ser Ile Ala Asp Tyr Met Lys Asp Asn Asn Leu Gly Ser 500 505 510 Tyr Thr Leu Gly Pro Thr Ala Val Asn Pro Leu Glu Leu Ser Asn Val 515 520 525 Ala Ala Thr Ile Ala Ser Gly Gly Met Trp Cys Glu Pro Asn Pro Ile 530 535 540 Ala Ser Val His Asp Arg Glu Gly Asn Glu Val Tyr Ile Asp Arg Pro 545 550 555 560 Ala Cys Glu Arg Ala Ile Asp Ala Glu Thr Ala Ser Ala Leu Ala Val 565 570 575 Gly Met Ser Lys Asp Thr Val Ser Gly Thr Ala Ala Ser Ala Ala Ser 580 585 590 Met Tyr Gly Trp Ser Leu Pro Thr Ala Ala Lys Thr Gly Thr Thr Glu 595 600 605 Ser Asn Gln Ser Ser Ala Phe Met Gly Phe Asn Ser Asn Phe Ala Ala 610 615 620 Ala Pro Tyr Ile Tyr Asn Asp Gly Thr Ser Thr Thr Pro Leu Cys Ser 625 630 635 640 Gly Pro Val Arg Gln Cys Ser Ser Gly Asn Leu Phe Gly Gly Asn Glu 645 650 655 Pro Ala Gln Thr Trp Phe Asn Met Ala Ser Asn Val Pro Ala Ala Ser 660 665 670 Gln Gly Thr Leu Pro Ser Ser Ser Asp Ser Phe Arg Leu Gly Thr Ser 675 680 685 Gly Glu Leu Leu Asn Gln Val Val Gly Gln Ser Glu Ala Ser Ala Arg 690 695 700 Arg Thr Leu Glu Ala Lys Gly Tyr Lys Val Thr Thr Arg Ser Val Ser 705 710 715 720 Gly Ala Gly Ser Ala Arg Gly Thr Val Val Ser Ala Thr Pro Gln Gly 725 730 735 Ala Val Leu Ile Asp Gly Gly Thr Val Ile Leu Asp Ile Ser Asp Gly 740 745 750 Thr Ser Pro Ala Pro Ala Ala Thr Asn Asn Asp Asp Ser Asp Asp Gly 755 760 765 Asp Thr Pro Ala Pro Ser Thr Asn Asn Arg Gly Thr Thr Ile Glu Asp 770 775 780 Ala Ile Asn Asp Ala Ile Asn Gln Phe Phe Arg 785 790 795

Claims (8)

Each encoding a polypeptide having an amino acid sequence shown in Table 1 / Column 2, wherein the nucleic acid molecule at the amino acid position shown in Table 1 / Column 4 encodes an amino acid different from the specific amino acid shown in the same column of Table 1 / Column 5 Isolated nucleic acid molecule. The isolated nucleic acid molecule of claim 1, wherein the nucleic acid molecule at the amino acid position shown in Table 1 / Column 4 encodes the amino acid shown in the same column of Table 1 / Column 6. A vector comprising at least one nucleic acid sequence as claimed in claim 1. A host cell transfected with at least one vector as claimed in claim 3. The host cell of claim 4, wherein the expression of said nucleic acid molecule regulates the production of fine chemicals from said cell. A method of producing a fine chemical, comprising producing a fine chemical by culturing the cells transfected with at least one vector as claimed in claim 3. The method of claim 6, wherein the fine chemical is an amino acid. 8. The method of claim 7, wherein said amino acid is lysine.