KR20050037283A - Antibiotics screening composition comprising streptococcus pneumoniae essential gene or its protein product, and antibiotics screening method using said composition - Google Patents

Abstract

The present invention relates to an antibiotic screening composition comprising an essential gene of pneumococcal or a protein expressed therefrom and an antibiotic screening method using the same.

Essential genes and proteins expressed therefrom included in the composition for screening of the present invention perform essential functions for the survival of pneumococci.

Therefore, the screening method using the composition of the present invention can be usefully used for the search and development of antibiotics that can effectively act against pathogenic bacteria, including pneumococci.

Description

Antibiotic screening composition comprising Streptococcus pneumoniae essential gene or its protein product, and antibiotics screening method using said composition}

The present invention relates to an antibiotic screening composition comprising an essential gene of Streptococcus pneumoniae or a protein expressed therefrom and an antibiotic screening method using the same.

The development of new antibiotics is an important field in that it can create enormous added value in the industrial economy, in addition to the clinical importance of the treatment of antibiotic resistant bacterial infections.

However, traditional methods of developing antibiotics, such as randomly searching for antibiotics from natural products or bacteria, or partially modifying the structure of a conventionally synthesized compound, have already faced limitations. There is almost no state.

In recent years, new technologies such as bioinformatics, microbial genomics, proteomics, and high throughput screening (HTS) have been developed to explore new antibiotic targets in pathogenic bacteria and to prevent them. Research is underway to develop materials.

At this time, a representative gene is a representative gene that can be a target gene, the essential gene is a gene essential for the survival or pathogenesis of bacteria. Thus, substances capable of inhibiting the expression mechanism of essential genes or the activity of proteins expressed from essential genes have the potential as ideal novel antibiotics. In addition, the substance inhibits the activity of genes and proteins that are required only for the growth of bacteria, and has the advantage of being safe in human toxicity.

In addition, the discovery of target genes will lead to the development of a new concept of antibiotics, which will provide the basis for genome-based drug development, creating a global new drug market and creating enormous industrial and economic added value.

However, since little information on the essential genes of a particular pathogen is not known so far, it is urgent to invent the discovery of the essential genes and the antibiotic screening method using the same.

In the present invention, by providing a composition for screening comprising the essential gene of pneumococcal, or a protein expressed therefrom and a screening method using the same, it is intended to establish a new antibiotic development system with effective antibacterial activity and human safety.

The present invention provides a composition for screening antibiotics comprising essential genes of pneumococcal.

The present invention, spr0003, spr0047, spr0081, spr0129, spr0131, spr0139, spr0145, spr0156, spr0173, spr0210, spr0243, spr0266, spr0267, spr0268, spr0269, spr03r, spr0338, spr0338 spr0393, spr0395, spr0478, spr0530, spr0585, spr0604, spr0626, spr0646, spr0666, spr0670, spr0671, spr0729, spr0755, spr0756, spr0788, spr0796, spr0797, spr0845, spr0856, 08 spr0978, spr0979, spr0982, spr0992, spr0996, spr1001, spr1024, spr1036, spr1063, spr1064, spr1065, spr1081, spr1111, spr1150, spr1158, spr1282, spr1312, spr1329, spr13r, spr13, 136 spr1411, spr1413, spr1419, spr1427, spr1429, spr1430, spr1432, spr1433, spr1434, spr1439, spr1458, spr1460, spr1468, spr1472, spr1502, spr1510, spr1511, spr1514, spr1515, sp115r spr1580, spr1603, spr1617, spr1632, spr1642, spr1647, spr1666, spr1671, spr1674, spr1696, spr16 97, spr1776, spr1797, spr1804, spr1825, spr1853, spr1856, spr1881, spr1882, spr1910, spr1914, spr1950, spr1963, spr1971, spr1973, spr1987, spr2012, spr2013, spr2027, and spr2034 It provides a composition for screening.

The present invention is SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 223, 225, 227, 229, 231, 233, 235, 237, 239, 241, 243, A nucleotide sequence described in 245, 247, or 249, or a base sequence in which one or more disruptions, deletions, insertions, points, substitutions, nonsenses, missenses, polymorphisms, or rearrangement mutations have occurred in the nucleotide sequences of these SEQ ID NOs; It provides a composition for antibiotic screening comprising at least one kind.

The present invention also provides a composition for screening antibiotics comprising at least one selected from proteins expressed from the genes.

The present invention, SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, A base sequence in which one or more disruptions, deletions, insertions, points, substitutions, nonsense, missense, polymorphisms, or rearrangement mutations have occurred in the amino acid sequence described in 246, 248, 250, or in the base sequences of the essential genes described above It provides a composition for screening antibiotics comprising at least one selected from polypeptide fragments expressed from All.

The present invention also provides an antibiotic screening method using an antibiotic screening composition comprising at least one selected from the essential genes of pneumococcus described above as a target material.

The present invention, after contacting an antibiotic screening composition comprising at least one of the essential genes of pneumococcus described above and the test substance, and confirming the reaction between them, the expression of the gene contained in the composition Provided is an antibiotic screening method comprising determining whether to exhibit inhibitory activity.

The present invention also provides an antibiotic screening method using an antibiotic screening composition comprising at least one selected from proteins expressed from essential genes of pneumococcus as described above.

The present invention comprises contacting an antibiotic screening composition comprising at least one selected from the proteins expressed from the essential genes of pneumococcus described above and the test substance, and then checking the reaction therebetween, wherein the composition comprises Provided is an antibiotic screening method comprising determining whether the protein exhibits activity that inhibits the function of the protein.

The protein expressed from the spr0003 gene is a conserved hypothetical protein whose function is not yet clearly identified.

The protein expressed from the spr0047 gene is amidophosphoribosyl transferase, an enzyme involved in purine biosynthesis, and 5-phospho-beta-D-ribosylamine (5-phospho-beta-D). The reaction of -ribosylamine with diphosphate and L-glutamate catalyzes the production of L-glutamine and 5-phospho-alpha-D-ribose-1-diphosphate.

The protein expressed from the spr0081 gene is ABC transporter membrane-spanning permease, which is involved in carbohydrate transport and metabolism.

The protein expressed from the spr0129 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

The protein expressed from the spr0131 gene is secreted metalloendopeptidase Gcp (secreted metalloendopeptidase Gcp), an enzyme involved in post-translational modification and turnover of the protein.

The protein expressed from the spr0139 gene is UDP-glucose dehydrogenase, which is particularly involved in cell envelope formation through biosynthesis of the cell envelope, and is specific for UDP-glucuronate. It acts as the main enzyme in the synthesis process.

The protein expressed from the spr0145 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

The protein expressed from the spr0156 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

The protein expressed from the spr0173 gene is arsenate reductase, which is involved in inorganic ion transport and metabolism, and catalyzes the conversion of arsenate to arsenite. It is a major enzyme involved in the heavy metal resistance of bacteria.

The protein expressed from the spr0210 gene is adenylate kinase (ATP-AMP transphosphorylase) involved in pyrimidine biosynthesis and catalyzes dephosphorylation mechanism.

The protein expressed from the spr0243 gene is prolyl-tRNA synthetase, which is involved in aminoacyl-tRNA synthesis and amino acid activation, and L-prolyl-tRNA from L-proline and tRNA. Catalyze the resulting reaction.

The protein expressed from the spr0266 gene is a dihydropteroate synthase that is involved in coenzyme metabolism, which is 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydride. Reaction of producing diphosphate and dihydrophteroate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate Catalyzes.

The protein expressed from the spr0267 gene is dihydrofolate synthetase, which is involved in coenzyme metabolism, and catalyzes the reaction of glutamate residues to folates in combination with folylpolyglutamate synthetase.

The protein expressed from the spr0268 gene is GTP cyclohydrolase, which is involved in coenzyme metabolism. It combines GTP and water molecules to formate and 2-amino-4-hydroxy-6- (erythri). Rho-1,2,3-trihydroxypropyl) dihydrophteridine triphosphate (2-amino-4-hydroxy-6- (erythro-1,2,3-trihydroxypropyl) dihydropteridine triphosphate) Catalyzes.

The protein expressed from the spr0269 gene is aldolase-pyrophosphokinase, which is involved in coenzyme metabolism, 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydro Terrydine (2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine) is a 2-amino-7,8-dihydro-4-hydroxy-6- (diphosphooxymethyl) pteridine ( Catalyzes the conversion to 2-amino-7,8-dihydro-4-hydroxy-6- (diphosphooxymethyl) pteridine).

The protein expressed from the spr0304 gene is penicillin-binding protein 2X (penicillin-binding protein 2X), which is essential for bacterial cell wall biosynthesis, cell division, and cell elongation and is the target enzyme of beta lactam antibiotics. One of the proteins (PBPs).

The protein expressed from the spr0338 gene is mevalonate kinase, which is involved in lipid metabolism, and catalyzes the formation of mevalonate 5-phosphate, a major factor in cholesterol biosynthesis, by phosphorylating mevalonic acid. do.

The protein expressed from the spr0356 gene is mannitol PTS EII, which is involved in carbohydrate transport and metabolism, and acts as a transporter in catabolism of polyhydric alcohol-mannitol.

The protein expressed from the spr0359 gene is mannitol-1-phosphate-5-dehydrogenase, which is involved in carbohydrate transport and metabolism, and D-mannitol-1-phosphate Catalyze the reaction to convert to D-fructose-6-phosphate.

The protein expressed from the spr0372 gene is a seryl-tRNA synthetase involved in translation and ribosomal structure biogenesis, which produces L-seryl-tRNA by binding L-serine and tRNA. Catalyze the reaction.

The protein expressed from spr0393 gene is glutamyl tRNA-Gln amidotransferase subunit B, which is involved in the synthesis and amino acid activation of aminoacyl tRNA.

The protein expressed from the spr0395 gene is tRNA-Gln amidotransferase subunit C), which is involved in the synthesis and amino acid activation of aminoacyl tRNAs.

The protein expressed from the spr0478 gene is a transcription elongation factor, which is part of the ribosomal antitermination complex involved in transcription prolongation, and the elongation of RNA polymerase. It is an enzyme that prevents Rho-dependent premature termination by increasing the rate.

The protein expressed from the spr0530 gene is fructose-bisphosphate aldolase, which is involved in carbohydrate transport and metabolism. ) And catalyzes the reaction to produce D-glyceraldehyde 3-phosphate.

The protein expressed from the spr0585 gene is thymidylate synthase, which is involved in nucleotide transport and metabolism, in which 5,10-methylenetetrahydrofolate is converted to dihydrofolate. Catalyzes.

The protein expressed from the spr0604 gene is undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase (undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase) involved in extracellular membrane biosynthesis, UDP-Mur2Ac (oyl- By reaction of L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) with undecaprenyl phosphate Mur2Ac (oyl-L-Ala-gamma-D-Glu-L-Lys- D-Ala-D-Ala) -diphosphoundecaprenol catalyzes the reaction produced.

Proteins expressed from the spr0626 gene are lysyl-tRNA synthetase or lysine-tRNA ligase involved in translation and ribosomal structure biogenesis, and L-lysine and tRNA Catalyzes the reaction by which L-lysyl-tRNA is produced.

The protein expressed from the spr0646 gene is phospho-beta-gluco or galactosidase involved in carbohydrate metabolism and catalyzes the hydrolysis reaction of beta-D-glucose.

Proteins expressed from the spr0666 gene are ABC transporter ATP-binding protein & cell division protein, which are involved in cell division and transport. They are located in the inner membrane and are of the type ATP-binding cassette (ABC). Act as a carrier. The protein forms a dimer complex through binding to FtsX, an integral membrane protein, and is also involved in the transport of salts.

The protein expressed from the spr0670 gene is an ATP-dependent RNA helicase involved in DNA replication, which causes unwinding of double DNA in an ATP-dependent manner.

The protein expressed from the spr0671 gene is S-adenosylmethionine synthetase, which is involved in coenzyme metabolism, and converts L-methionine to S-adenosyl-L-methionine.

The protein expressed from the spr0729 gene is an enzyme involved in coenzyme metabolism. It is a methylenetetrahydrofolate dehydrogenase that converts 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate. It also acts as a function and simultaneously performs the function of methenyltetrahydrofolate cyclohydrolase, which converts 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.

The protein expressed from the spr0755 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

The protein expressed from the spr0756 gene is topoisomerase IV subunit B, which is involved in DNA replication, and is part of an enzyme that releases the double-helix structure of newly created chromosomes.

The protein expressed from the spr0788 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

The protein expressed from the spr0796 gene is 6-phosphofructokinase I, which is involved in carbohydrate transport and metabolism. D-fructose 6-phosphate is converted to D-fructose 1,6-bisphosphate. Catalyze the reaction to convert.

The protein expressed from the spr0797 gene is pyruvate kinase I, which is involved in carbohydrate transport and metabolism, catalyzing the conversion of pyruvate to phosphoenolpyruvic acid.

The protein expressed from the spr0845 gene is a UMP kinase involved in nucleic acid transport and metabolism, and acts as a phosphotransferase during pyrimidine biosynthesis.

The protein expressed from the spr0856 gene is the competence protein CelA (carpetation protein CelA) involved in carbohydrate transport and metabolism, which catalyzes the conversion of protein N-phosphate histidine and sugar into protein histidine and sugar phosphate.

The protein expressed from the spr0861 gene is the translation initiation factor IF-3 (IF-3) involved in the translation process.

The protein expressed from the spr0881 gene is a competence protein CoiA (Copetence Protein CoiA), which is believed to be involved in the DNA uptake process.

The protein expressed from the spr0882 gene is a group B oligopeptidase involved in amino acid transport and metabolism, which is leucyl aminopeptidase.

The protein expressed from the spr0906 gene is a lipoprotein involved in inorganic ion transport and metabolism. It is a major component of the cell's basement membrane and an important protein for the development and maintenance of cell structure.

The protein expressed from the spr0965 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

The protein expressed from the spr0972 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

The protein expressed from the spr0978 gene is a DNA primase involved in DNA replication, and recognizes a specific gene sequence to synthesize a small primer RNA that acts as an initiator at the beginning of replication.

The protein expressed from the spr0979 gene is RNA polymerase sigma factor 70, which is involved in the transcription process, and is one of the subunits constituting the DNA-dependent RNA polymerase.

The protein expressed from the spr0982 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

The protein expressed from the spr0992 gene is a methionine aminopeptidase involved in the translation process and acts as a peptidase of the N-terminal methionine of the resulting protein.

The protein expressed from the spr0996 gene is a DNA repair protein involved in DNA replication and repair. It is a major enzyme that repairs DNA damage that occurs during replication.

The protein expressed from the spr1001 gene is pyridoxal-phosphate dependent aminotransferase and is involved in amino acid transport and metabolism.

The protein expressed from the spr1024 gene is a DNA ligase that is involved in DNA replication, recombination and repair. It is an enzyme that connects the 5- and 3-terminal strands of adjacent DNA strands with phosphodiester bonds. .

The protein expressed from the spr1036 gene is enolase, an enzyme involved in carbohydrate transport and metabolism, which catalyzes the conversion of 2-phospho-D-glycerate to phosphoenolpyruvic acid.

The protein expressed from the spr1063 gene is a histidine-containing phosphocarrier protein involved in carbohydrate transport and metabolism. It is one of the enzymes in the phosphate system. Catalyze the reaction converted to fructose phosphate.

The protein expressed from the spr1064 gene is glutaredoxin-like protein involved in electron transport system for ribonucleotide reductase system NrdEF, which is involved in post-translational modification and protein conversion, and is involved in ribonucleic acid It acts as a reductase of diphosphate or triphosphate.

The protein expressed from the spr1065 gene is ribonucleoside-diphosphate reductase, which is involved in nucleic acid transport and metabolism. And catalyzes the conversion of oxidized thioredoxin to ribonucleoside diphosphate and reduced thioredoxin.

The protein expressed from the spr1081 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

The protein expressed from the spr1111 gene is similar to the N-terminal region of the Dfp protein. The N-terminal portion of the Dfp protein is involved in coenzyme metabolism (R) -4 '. Catalyzes the reaction in which phospho-N-pantothenoylcysteine ((R) -4'-phospho-N-pantothenoylcysteine) is decarboxylated to 4'-phosphopantetheine.

The protein expressed from the spr1150 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

The protein expressed from the spr1158 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

The protein expressed from the spr1282 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

The protein expressed from the spr1312 gene is thioredoxin reductase, which is involved in post-translational modification and protein conversion, and catalyzes the reaction of converting thioredoxin to thioredoxin disulfide.

The protein expressed from the spr1329 gene is a glycyl-tRNA synthetase alpha chain involved in aminoacyl tRNA synthesis, which catalyzes the synthesis of diphosphate and glycyl-tRNA from glycine and tRNA. It is a subunit of enzyme.

The protein expressed from the spr1359 gene is a proton-translocating ATPase (sector, epsilon-subunit) of the F1 portion of the F ₀ F ₁ type proton translocation ATPase, which converts ATP into ADP and phosphate. do.

The protein expressed from the spr1360 gene is a beta subunit of the F ₀ F ₁ type proton translocation ATPase F1 moiety that converts ATP into ADP and phosphate, and is involved in energy production and conversion.

The protein expressed from the spr1361 gene is a gamma subunit of the F ₀ F ₁ type proton translocation ATPase F1 moiety that converts ATP into ADP and phosphate, and is involved in energy production and conversion.

The protein expressed from the spr1362 gene is an alpha subunit of the F ₀ F ₁ type proton translocation ATPase F1 moiety that converts ATP into ADP and phosphate and is involved in energy production and conversion.

The protein expressed from the spr1364 gene is the B subunit of the F ₀ F ₁ type proton translocation ATPase F1 moiety that converts ATP into ADP and phosphate, and is involved in energy production and conversion.

The protein expressed from the spr1409 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

The protein expressed from the spr1410 gene is a P-type ATPase-calcium transporter, an enzyme involved in inorganic ion transport and metabolism, and is an ATP agent involved in calcium transport.

The protein expressed from the spr1411 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

The protein expressed from the spr1413 gene is tRNA nucleotidyltransferase, an enzyme that is involved in ribosomal structure and biosynthesis at the transfer stage.

Proteins expressed from the spr1419 gene are hypothetical proteins that have not yet been clearly identified and conserved.

The protein expressed from the spr1427 gene is an ATP-dependent Clp protease ATP-binding subunit involved in post-translational modification and protein conversion.

The protein expressed from the spr1429 gene is dihydrofolate reductase, which is involved in coenzyme metabolism, and converts 5,6,7,8-tetrahydrofolate into 7,8-dihydrofolate. Catalyzes.

The protein expressed from the spr1430 gene is a hydrogen peroxide resistance protein, an enzyme that is involved in inorganic ion transport and metabolism.

The protein expressed from the spr1432 gene is triose phosphate isomerase, which is involved in carbohydrate transport and metabolism.

The protein expressed from the spr1433 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

The protein expressed from the spr1434 gene is a homoserine O-succinyltransferase involved in amino acid transport and metabolism. Succinyl CoA and L-homoserine are coA and O-succinyl-L-homocysteine. Catalyze the reaction to convert to.

The protein expressed from the spr1439 gene is a transcriptional pleiotropic represso, an enzyme involved in transcriptional regulation.

The protein expressed from the spr1458 gene is ferredoxin, which is involved in energy generation and conversion, and is an iron-sulfur containing protein that acts in the intracellular electron transfer process.

The protein expressed from the spr1460 gene is UDP-glucose 4-epimerase, an enzyme involved in the biosynthesis of the cell envelope, and catalyzes the conversion of UDP-glucose to UDP-galactose.

The protein expressed from the spr1468 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

The protein expressed from the spr1472 gene is threonyl-tRNA synthetase 1, which is involved in aminoacyl-tRNA synthesis during translation and synthesizes L-threonyl-tRNA from L-threonine and tRNA.

The protein expressed from the spr1502 gene is isoleucyl-tRNA synthetase, which is involved in aminoacyl-tRNA synthesis during translation and synthesizes L-isoleucine-tRNA from L-isoleucine and tRNA.

The protein expressed from the spr1510 gene is the cell division protein FtsZ (FtsZ), a GTPase involved in cell division and chromosome partitioning.

The protein expressed from the spr1511 gene is the cell division protein FtsA (FtsA), which is an ATPase involved in cell division and chromosome partitioning.

The protein expressed from the spr1514 gene is UDP-N-acetylmuramoylalanine-D-glutamyl-lysine-D-alanyl-D-alanine ligase (UDP-N-acetylmuramoylalanine-D), an enzyme involved in cell envelope biosynthesis. -glutamyl-lysine-D-alanyl-D-alanine ligase) from UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine and D-alanyl-D-alanine Catalyzes the reaction to synthesize UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine.

The protein expressed from the spr1515 gene is D-alanine-D-alanine ligase, an enzyme involved in cell envelope biosynthesis.

The protein expressed from the spr1535 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

The protein expressed from the spr1540 gene is an alanine racemase involved in cell envelope biosynthesis and catalyzes the conversion of L-alanine to D-alanine.

The protein expressed from the spr1541 gene is an acyl carrier protein synthase involved in fat metabolism and synthesizes holo-ACP.

The protein expressed from the spr1553 gene is phosphoglycerate dehydrogenase, which is involved in amino acid transport and metabolism, and converts 3-phosphoglycerate to 3-phosphohydroxypyruvate. Catalyzes.

The protein expressed from the spr1555 gene is a primosome component, which is one of the components of the protein complex involved in the synthesis of a primer during DNA replication.

The protein expressed from the spr1571 gene is 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase, which is involved in fat metabolism. Synthesize 3-methylglutaryl coenzyme A.

The protein expressed from the spr1580 gene is methionyl-tRNA formyltransferase, which is involved in translation, converting L-methionyl-tRNA into tetrahydrofolate and N-formylethionyl-tRNA. To catalyze the reaction.

The protein expressed from the spr1603 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

The protein expressed from the spr1617 gene is sucrose-6-phosphate hydrolase involved in carbohydrate transport and metabolism.

Proteins expressed from the spr1632 gene are tryptophan synthase beta chains that are involved in amino acid transport and metabolism. L-serine and 1- (indol-3-yl) glycerol 3-phosphate (1- (indol-) Catalyzes the conversion of 3-yl) glycerol 3-phosphate) to L-tryptophan and glyceraldehyde 3-phosphate.

The protein expressed from the spr1642 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

The protein expressed from the spr1647 gene is UDP-glucose 4-epimerase involved in cell envelope biosynthesis, which catalyzes the conversion of UDP-glucose to UDP-galactose.

The protein expressed from the spr1666 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

The protein expressed from the spr1671 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

Proteins expressed from the spr1674 gene are hypothetical proteins that have not yet been clearly identified and conserved.

The protein expressed from the spr1696 gene is glutamate racemase involved in cell envelope biosynthesis and catalyzes the conversion of L-glutamate to D-glutamate.

The protein expressed from the spr1697 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

The protein expressed from the spr1776 gene is a DNA-dependent RNA polymerase involved in transcription, an enzyme that makes diphosphate and RNA from nucleoside triphosphate.

Proteins expressed from the spr1797 gene are pentose-5-phosphate-3-epimerase, an enzyme that functions in the pentose phosphate pathway, which is involved in carbohydrate transport and metabolism.

The protein expressed from the spr1804 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

The protein expressed from the spr1825 gene is glyceraldehyde 3-phosphate dehydrogenase, which is involved in carbohydrate transport and metabolism. D-glyceraldehyde 3-phosphate is substituted with Catalyze the reaction to convert to.

The protein expressed from the spr1853 gene is a ribonuclease P-protein component involved in translation, which is a 5'-extra-nucleotide from a tRNA precursor. Remove

The protein expressed from the spr1856 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

The protein expressed from the spr1881 gene is glutamyl-tRNA synthetase, which is involved in aminoacyl-tRNA synthesis during translation and synthesizes L-glutamyl-tRNA from L-glutamate and tRNA.

The protein expressed from the spr1882 gene is glucose-6-phosphate isomerase involved in carbohydrate transport and metabolism.

The protein expressed from the spr1910 gene is tyrosyl-tRNA synthetase 1, which is involved in aminoacyl-tRNA synthesis during translation and synthesizes L-tyrosyl-tRNA from L-tyrosine and tRNA.

The protein expressed from the spr1914 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

The protein expressed from the spr1950 gene is a hypothetical protein that has not yet been clearly identified and has been conserved.

Proteins expressed from the spr1963 gene are probable alcohol dehydrogenases that are involved in energy production and conversion, and break down alcohol.

The protein expressed from the spr1971 gene is a fucose pathway protein (function unknown), involved in carbohydrate transport and metabolism.

The protein expressed from the spr1973 gene is a fucose kinase, which is involved in carbohydrate transport and metabolism.

The protein expressed from the spr1987 gene is a truncation of a hypothetical protein that has not yet been clarified in function.

The protein expressed from the spr2012 gene is a competence factor ComFC (competence factor ComFC), an enzyme involved in transformation, and acts as a competence factor to accept external DNA.

The protein expressed from the spr2013 gene is a competence factor ComFA, an enzyme involved in transformation, and acts as a competence factor that accepts external DNA and acts as an ATP-dependent RNA / DNA helicase. ) Also affects the function.

The protein expressed from the spr2027 gene is phosphotidylglycerophosphate synthase, which is involved in fat metabolism. It is 3- (3-phosphatidyl from CDP-diacylglycerol and glycerol 3-phosphate. Catalyze the reaction to synthesize) -glycerol 1-phosphate.

The protein expressed from the spr2034 gene is tryptophanyl-tRNA synthetase, which is involved in aminoacyl-tRNA synthesis during translation and synthesizes L-tryptophanyl-tRNA from L-tryptophan and tRNA.

The necessity of these genes for pneumococcus is described below.

In order to confirm that the genes are essential for the growth of pneumococcus, two PCRs are first performed on each gene to prepare a cassette in which the gene is deleted (FIG. 1).

In the primary PCR, a total of three primary PCR products are obtained by amplifying the other genes to replace each gene, the flanking region and the right side region of each gene.

Genes to replace each gene in the present invention using a marker gene that allows you to obtain a trait that was not present in the original pneumococcal, it is possible to confirm on the selection medium whether the substituted gene in the subsequent process is properly inserted into the genome of the transformed strain Make sure In the present invention, the aphIII gene (hereinafter referred to as 'KanR'), which has resistance to kanamycin, is used.

Of the primers used in the present invention, the reverse primers used for PCR of the left side region of each gene and the forward primers used for PCR of the right side region respectively represent the 5 'end portion of KanR and the 3' end portion of KanR, respectively. It is included.

In the secondary PCR, PCR reactions are performed on the primary PCR products using the forward primer used for the PCR on the left side and the reverse primer used for the PCR on the right side. Obtain nucleotides.

The finally obtained polynucleotide has KanR inserted into each original gene region, and the gene is deleted, and the left and right portions thereof are preserved as it is, and then used as a cassette to be inserted into the genome of the strain.

In order to delete each gene in the strain genome using the cassette prepared above, the cassette is used to transform pneumococci. At this time, the selective medium for culturing the transformed strain is prepared by the addition of kanamycin.

When transformation occurs, as shown in Figure 2, each gene existing on the genome of the bacteria is replaced by KanR in the cassette introduced from the outside by the homologous recombination mechanism, consequently the target gene on the genome Is knocked out.

If the deleted gene is an essential gene, the transformed strain cannot form a colony in the selection medium because it is impossible to grow (FIG. 3). On the other hand, if the deleted gene is a non-essential gene, the transformed strain is able to survive without the target gene, thereby forming a colony in the selection medium (FIG. 3).

Transgenic strains each of which the genes of the present invention were deleted do not grow in kanamycin-containing selective medium. That is, since the genes of the present invention are essential genes necessary for the growth of pneumococcal, if any one of the genes is deleted or the function of the protein expressed therefrom becomes fatal, the growth of pneumococcal is severely affected.

Using this principle, in the present invention, antibiotics can be screened using the composition of the present invention including the essential gene of pneumococcal or a protein expressed therefrom as a target material.

The screening method of the present invention comprises contacting the composition with a test substance, and then confirming a reaction therebetween to determine whether to inhibit the expression of the gene or to inhibit the function of the protein.

In the screening method of the present invention, confirmation of the reaction between the composition containing each essential gene and the test substance may use conventional methods used to confirm the reaction between DNA-DNA, DNA-RNA, and DNA-protein.

For example, a hybridization test for confirming the binding between the gene and the test substance in vitro, a method of measuring the expression rate of the gene through Northern analysis after reacting pneumococcal cells and the test substance, Alternatively, the reporter gene may be linked to the gene, introduced into the bacterial cell, reacted with the test substance, and a method of measuring the expression rate of the reporter protein may be used.

In this case, the composition of the present invention may include distilled water or a buffer to stably maintain the structure of the nucleic acid in addition to the essential gene.

In the screening method of the present invention, for confirming the reaction between the composition containing the protein expressed from the essential gene and the test substance, conventional methods used to confirm the reaction between the protein and the protein may be used.

For example, a method of measuring enzyme activity after reacting the protein with a test substance, or a yeast double hybrid method (yeast two-hybird) may be used.

In this case, the composition of the present invention may include a buffer or a reaction solution that stably maintains the structure or physiological activity of the protein, in addition to the protein expressed from the essential gene. In addition, the composition of the present invention may include pneumococcal pneumoniae expressing the protein, or pneumococci containing the plasmid expressing the protein under a promoter capable of controlling transcription rate for in vivo experiments.

In the screening method of the present invention, the test substance may be individual nucleic acids, proteins, other extracts, or natural products that are assumed to have antibiotic potential or randomly selected according to a conventional selection method. Chemical libraries are used to enable mass retrieval in a short time.

The chemical library is a combination of thousands and tens of thousands of chemicals having various structures on a solid chip. When the composition of the present invention is reacted with a chemical library, the chemical screening method is a conventional screening method of only 1: 1 confirmation. Compared with the antimicrobial candidates, it is possible to detect candidates that have an antibiotic effect much faster and more effectively.

The candidate material obtained by searching the chemical library acts as a leading compound in the future development of antibiotics, so that the inhibitory effect of the gene of the present invention or the protein expressed therefrom may be expressed on the leading material. By modifying and optimizing its structure, new antibiotics can be developed.

The substance thus obtained shows a partial or complete activity inhibitory effect on the essential gene of pneumococcal pneumoniae or a protein expressed therefrom, so that the growth of pneumococci can be suppressed. In addition, pneumococcal and other pathogenic bacteria similar in their gene expression structure may have a growth inhibitory effect.

Therefore, the composition of the present invention and the screening method using the same can be usefully used for the search and development of antibiotics against pathogenic bacteria, including pneumococci.

Hereinafter, the present invention will be described in more detail, but the scope of the present invention is not limited to the following examples.

EXAMPLES Confirmation of Essentials of Genes Used in the Present Invention

In order to confirm the necessity of the genes used in the present invention, the deletion cassettes of the genes were prepared as follows. After deletion of the genes of the present invention in pneumococci, the effects were observed.

1) Preparation of deletion cassette of gene of the present invention

A deletion cassette of the gene of the present invention and a deletion cassette of the non-essential gene spr0476 to be used as a comparative group were prepared as follows.

For cassette preparation, KanR having kanamycin resistance, and primers capable of amplifying the left side region and the right side region of the gene of interest were prepared.

The base sequences of the primers used for the PCR of KanR were prepared as SEQ ID NOs: 251 and 252.

The primers used for each gene of pneumococcal are forward primer (LF) and reverse primer (LR) for amplification of the left side region, and forward primer (RF) and reverse primer (RR) for amplification of the right side region. The total of four was made. In this case, the reverse primer (LR) of the left side region includes 21 nucleotide sequences (5'-GACGAACTCCAATTCACTGTT-3 ') complementary to the promoter region of the 5' side of KanR, and the forward primer (RF) of the right side region. ) Was constructed to include 21 nucleotide sequences (5'-AGATTTAGATGTCTAAAAAGC-3 ') complementary to the 3' end of KanR.

As described above, the primers used for each gene are SEQ ID NOs 253, 254, 255 and 256 for spr0003 (the order is LF, LR, RF, RR in the following order), and for spr0047 257, 258, 259, 260, 261, 262, 263, 264 for spr0081, 265, 266, 267, 268 for spr0129, 269, 270, 271, 272 for spr0131, 273, 274, 275, 276 for spr0139, 277, 278 for spr0145 , 279, 280, 281, 282, 283, 284 for spr0156, 285, 286, 287, 288 for spr0173, 289, 290, 291, 292 for spr0210, 293, 294, 295, 296, spr0266 297, 298, 299, 300, 301, 302, 303, 304 for spr0267, 305, 306, 307, 308 for spr0268, 309, 310, 311, 312 for spr0269, 313, 314, for spr0304 315, 316, spr0338 317, 318, 319, 320, spr0356 321, 322, 323, 324, spr0359 325, 326, 327, 328, spr0372 329, 330, 331, 332, spr0393 Case 333, 334, 335, 336, 337, 338, 339, 340 for spr0395 341, 342 for spr0478 , 343, 344, spr0530 345, 346, 347, 348, spr0585 349, 350, 351, 352, spr0604 353, 354, 355, 356, spr0626 357, 358, 359, 360, spr0646 For 361, 362, 363, 364, for spr0666 365, 366, 367, 368, for spr0670 369, 370, 371, 372, for spr0671 373, 374, 375, 376, for spr0729 377, 378, 379, 380, 381, 382, 383, 384 for spr0755, 385, 386, 387, 388 for spr0756, 389, 390, 391, 392, 393, 394, 395, 396, spr0797 for spr0788 Case 397, 398, 399, 400, spr0845 401, 402, 403, 404, spr0856 405, 406, 407, 408, spr0861 409, 410, 411, 412, spr0881 413, 414, 415 , 416, spr0882 417, 418, 419, 420, spr0906 421, 422, 423, 424, spr0965 425, 426, 427, 428, spr0972 429, 430, 431, 432, spr0978 433, 434, 435, 436, spr0979 437, 438, 439, 440, spr0982 441, 442, 443, 444, spr0992 445, 446, 447, 448, 449, 450, 451, 452 for spr0996, 453, 454, 455, 456 for spr1001, 457, 458, 459, 460 for spr1024, 461, 462, 463, 464 for spr1036, 465, 466 for spr1063 , 467, 468, spr1064 469, 470, 471, 472, spr1065 473, 474, 475, 476, spr1081 477, 478, 479, 480, spr1111 481, 482, 483, 484, spr1150 For 485, 486, 487, 488, For spr1158 489, 490, 491, 492, For spr1282 493, 494, 495, 496, For spr1312 497, 498, 499, 500, For spr1329 501, 502, 503, 504, spr1359 505, 506, 507, 508, spr1360 509, 510, 511, 512, spr1361 513, 514, 515, 516, spr1362 517, 518, 519, 520, spr1364 Case 521, 522, 523, 524, spr1409 525, 526, 527, 528, spr1410 529, 530, 531, 532, spr1411 533, 534, 535, 536, spr1413 537, 538, 539 , 540, 542, 543, 544 for spr1419, 545, 546, 547, 548 for spr1427, 549, 550, 551, 552, spr1430 for spr1429 553, 554, 555, 556, 557, 558, 559, 560, spr1433 561, 562, 563, 564, spr1434 565, 566, 567, 568, spr1439 569, 570, 571 , 572, 573, 574, 575, 576 for spr1458, 577, 578, 579, 580 for spr1460, 581, 582, 583, 584, 583, 584 for spr1468, 585, 586, 587, 588, spr1502 589, 590, 591, 592, 593, 594, 595, 596, spr1511, 597, 598, 599, 600, spr1511, 601, 602, 603, 604, spr1515 605, 606, 607, 608, 610, 610, 611, 612 for spr1535, 613, 614, 615, 616 for spr1540 617, 618, 619, 620 for spr1541 621, 622, 623, 624 for spr1553 and 625 for spr1555 , 626, 627, 628, 629, 630, 631, 632, 633, 634, 635, 636, spr1603, 637, 638, 639, 640, spr1617 641, 642, 643, 644 for spr1571 , 645, 646, 647, 648 for spr1632, 649, 650, 651, 652 for spr1642, 653, 654, 655, 656 for spr1647, 657, 6 for spr1666 58, 659, 660, 661, 662, 663, 664 for spr1671, 665, 666, 667, 668, 669, 670, 671, 672 for spr1696, 673, 674, 675, 676 for spr1697 677, 678, 679, 680, spr1797 for spr1776 681, 682, 683, 684, 685, 686, 687, 688 for spr1804, 689, 690, 691, 692 for spr1825, 693, 694 for spr1853 , 695, 696, spr1856 697, 698, 699, 700, spr1881 701, 702, 703, 704, spr1882 705, 706, 707, 708, spr1910 709, 710, 711, 712, spr1914 713, 714, 715, 716, 717, 718, 719, 720, spr1950 721, 722, 723, 724, spr1971 725, 726, 727, 728, spr1973 729, 730, 731, 732, spr1987 733, 734, 735, 736, spr2012 737, 738, 739, 740, spr2013 741, 742, 743, 744, spr2027 745, 746, 747, 748, spr2034 In the case of 749, 750, 751, 752 was prepared to have the base sequence.

Among the primers, Kan F and Kan R were first used, and KanR was amplified using chromosomal DNA of Staphylococcus aureus as a template. And the left side region was amplified using LF primer and LR primer for each gene, and the right side region was amplified using RF primer and RR primer.

The PCR reaction was repeated 30 times under conditions of 1 minute at 94 ° C, 1 minute at 55 ° C, and 1 minute at 72 ° C.

In order to connect the three kinds of primary PCR products thus obtained into one polynucleotide, secondary PCR was performed.

Since 21 nucleotide sequences of the 3 'terminal region of the left region PCR product of each gene are complementary to the 5' terminal region of KanR, complementary strands can be synthesized by PCR. In addition, since 21 nucleotide sequences of the 5 'terminal region of the right region PCR product of each gene are complementary to the 3' terminal region of KanR, complementary strands can be synthesized by PCR.

Using this principle, secondary PCR was performed using L-F primers and R-R primers on three types of PCR products obtained in the first PCR reaction. The secondary PCR reaction was repeated 30 times under conditions of 1 minute at 94 ° C, 1 minute at 55 ° C, and 2 minutes at 72 ° C.

As a result, KanR was inserted into the original gene region to delete the original gene, and finally the deletion cassette was obtained in which the left and right portions of the original gene were preserved as they are.

For the deletion cassette for spr0476 to be used as a control, amplify the left flank of spr0476 using spr0476 LF (SEQ ID NO: 753) and spr0476 LR (SEQ ID NO: 754), and spr0476 RF (SEQ ID NO: 755) and spr0476 RR 756) was used to amplify the right flank of spr0476. Secondary PCR was performed on these primary PCR products in the same manner as described above, and the deletion cassette of the spr0476 gene in which KanR was inserted into the original spr0476 gene region, the target gene was deleted, and the left and right regions of the spr0476 gene were preserved as it is. Finally obtained.

2) Knockout of Target Gene Using Gene Deletion Cassette

Each of the genes of interest was knocked out by transforming pneumococci using the gene deletion cassette prepared in 1) as follows.

In this example, pneumococcal D39 strain and R6 strain were selected as strains to be used for transformation.

The strains, which were stored frozen, were inoculated in Todd-Hewitt agar medium containing 0.5% yeast extract, and the bacteria were cultured in an incubator maintained at 5-10% CO ₂ . Each strain was incubated overnight in 10 ml agar medium, and then 5 ml of this culture solution was added to 45 ml fresh agar medium and incubated at 37 ° C until the absorbance at 600 nm was 0.25. At this time, the incubation time was about 4-5 hours. Glycerol (glycerol) was added to the bacterial culture to a final concentration of 10%, and each aliquot was aliquoted and frozen in a mixture of dry ice and ethanol, and stored at -80 ° C until the transformation experiment.

Pneumococcal transformation was performed as follows.

First, 1 μg of DNA and 200 μl of transformed cells were diluted in a ratio of 1:10 in a transformation medium (Infant-Hewitt medium containing 0.5% yeast extract, 0.2% BSA, and 0.01% calcium chloride). It was. After diluting the solution for about 1 hour at 37 ° C., 100 ng / ml peptide pheromone Csp-1 (EMRLSKFFRDFILQRKK) was added. Thereafter, incubated at 37 ° C. for about 3 hours, plated on a selective medium containing 400 μg / ml kanamycin, and incubated at 37 ° C. for 48 hours.

3) Confirmation of the essentiality of the gene of the present invention

In order to confirm the essentiality of the gene of the present invention, it was observed whether the transforming strains of 2) grow in the kanamycin-containing medium.

For example, in FIG. 4, the group in which the spr0003 gene was deleted (left) is compared with the control group (right). As shown in Figure 4, in the case of the group of the spr0003 gene deletion of the present invention, unlike the control group, no colonies were produced. The rest of the genes showed the same result.

Thus, it can be seen that the genes of the present invention are essential genes necessary for the survival of pneumococcal.

In order to confirm that the substitution of KanR instead of spr0476 occurred in the genome of these colonies at random, several colonies were randomly selected and confirmed to be knocked out. As a result, KanR was inserted in both the transformed pneumococcal D39 and R6 strains. It could be confirmed (Fig. 5).

Therefore, the method of using the genes of the present invention as a target may be a good method for the development of new antibiotics.

Essential genes contained in the composition for screening of the present invention and proteins expressed therefrom are essential for the survival of pneumococci.

Therefore, the screening method of the present invention can be usefully used for the search and development of effective antibiotics against pneumococci and, moreover, pathogenic bacteria.

1 is a diagram showing the manufacturing process of the cassette for deleting the essential gene of pneumococcal.

Figure 2 is a diagram showing the process of deleting the essential gene of pneumococci through transformation.

Figure 3 is a diagram showing the process of confirming the deletion of essential genes by culturing the pneumococcal transforming strains in which a specific gene is deleted in a selective medium.

Figure 4 is a diagram confirming the growth in the selection medium of the pneumococcal transgenic strains deleted the essential gene of the present invention.

Figure 5 is a diagram confirming the insertion of the deletion cassette in the pneumococcal transforming strain, which deleted the non-essential gene spr0476 gene.

<110> SONG, Jae Hoon <120> Antibiotics screening composition comprising Streptococcus pneumoniae essential genes or its protein products, and antibiotics screening method using said composition <130> 03P-72 <160> 756 <170> KopatentIn 1.71 <210> 1 <211> 192 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (192) <223> Streptococcus pneumoniae spr0003 gene <400> 1 atgtatcaag ttggaaattt tgttgagatg aaaaaaccac acgcttgtac aatcaagtcg 60 actggtaaaa aggctaatcg ttgggaaatt acacgtgtag gagcagatat caaaataaaa 120 tgcagtaatt gtgagcatgt tgtcatgatg gggcgatatg attttgagcg aaaaatgaat 180 aaaattattg ac 192 <210> 2 <211> 64 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (64) <223> conserved hypothetical protein <400> 2 Met Tyr Gln Val Gly Asn Phe Val Glu Met Lys Lys Pro His Ala Cys 1 5 10 15 Thr Ile Lys Ser Thr Gly Lys Lys Ala Asn Arg Trp Glu Ile Thr Arg 20 25 30 Val Gly Ala Asp Ile Lys Ile Lys Cys Ser Asn Cys Glu His Val Val 35 40 45 Met Met Gly Arg Tyr Asp Phe Glu Arg Lys Met Asn Lys Ile Ile Asp 50 55 60 <210> 3 <211> 1440 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1440) <223> Streptococcus pneumoniae spr0047 gene <400> 3 atgacatacg aagtaaaatc tcttaatgaa gaatgtggtg ttttcggtat ttggggacat 60 ccagatgctg ctaagttgac ctattttgga ctccacagtc ttcaacaccg tggtcaggag 120 ggggcaggaa tcctctccaa tgatcaagga caactgaagc ggcatcgcga tatgggtctt 180 ctatcagaag tcttcagaaa tcctgctaac ttagataaat tgacgggaac tggtgcgatt 240 gggcatgtgc gttacgcgac tgctggcgaa gcttctgtag ataatatcca gcccttcctc 300 ttccgttttc acgatatgca gtttggcttg gctcataatg gaaatctgac caatgcagcc 360 tctctcaaga aagaactgga acaaagagga gcaattttca gcgcgacttc ggactctgaa 420 atcttggctc acctcattcg tcgcagtcat aatcctagcc tgatgggcaa aatcaaggaa 480 gcgctcagcc ttgtcaaagg tggttttgcc tatatcttgc tgtttgagga caagttgatt 540 gcggctcttg acccaaatgg attccgaccg ctttcgattg gtaaaatggc taatggagca 600 gttgttgtat cttctgaaac ctgtgctttt gaggtcattg gtgccgagtg gattcgtgat 660 ttgaagccag gtgagattgt gatcattgat gacgagggca ttcagtatga cagctataca 720 gatgataccc agttggcggt ttgttctatg gagtatatct actttgctcg ccctgattct 780 aatatccacg gtgtcaatgt ccatacggca cgtaagagaa tgggagcgca attggcgcga 840 gaatttaagc atgaggcaga tattgtagtt ggtgtgccca attcttccct aagcgcggct 900 atgggatttg cggaagaagc aggcttacca aatgaaatgg gtctgatcaa aaaccaatac 960 acccagcgaa cttttatcca accgactcaa gaattgcggg agcaaggagt gcggatgaaa 1020 ctgtctgctg tttcgggtgt tgtcaaaggc aaacgtgtgg tcatggtgga tgattccatt 1080 gtacgtggaa caacctctcg tcgtatcgtt cagctcttga aagaagcggg tgcgactgag 1140 gttcacgttg ccattggaag tcctgcacta gcgtatccat gtttctacgg gattgatatc 1200 cagacccgtc aggagctgat tgcagccaat catacggtcg aagaaactcg ccaaatcatt 1260 ggtgcggaca gtatgactta tctttcaatt gatggcttga ttgagtcgat tggtatcgaa 1320 acagatgcgc cgaacggtgg tctctgtgtc gcttactttg acggtgacta cccaacgcct 1380 ctctatgact acgaagaaga ctatcgtaga agtttggaag aaaagaccag tttttacaag 1440 1440 <210> 4 <211> 480 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (480) <223> Amidophosphoribosyl transferase <400> 4 Met Thr Tyr Glu Val Lys Ser Leu Asn Glu Glu Cys Gly Val Phe Gly 1 5 10 15 Ile Trp Gly His Pro Asp Ala Ala Lys Leu Thr Tyr Phe Gly Leu His 20 25 30 Ser Leu Gln His Arg Gly Gln Glu Gly Ala Gly Ile Leu Ser Asn Asp 35 40 45 Gln Gly Gln Leu Lys Arg His Arg Asp Met Gly Leu Leu Ser Glu Val 50 55 60 Phe Arg Asn Pro Ala Asn Leu Asp Lys Leu Thr Gly Thr Gly Ala Ile 65 70 75 80 Gly His Val Arg Tyr Ala Thr Ala Gly Glu Ala Ser Val Asp Asn Ile 85 90 95 Gln Pro Phe Leu Phe Arg Phe His Asp Met Gln Phe Gly Leu Ala His 100 105 110 Asn Gly Asn Leu Thr Asn Ala Ala Ser Leu Lys Lys Glu Leu Glu Gln 115 120 125 Arg Gly Ala Ile Phe Ser Ala Thr Ser Asp Ser Glu Ile Leu Ala His 130 135 140 Leu Ile Arg Arg Ser His Asn Pro Ser Leu Met Gly Lys Ile Lys Glu 145 150 155 160 Ala Leu Ser Leu Val Lys Gly Gly Phe Ala Tyr Ile Leu Leu Phe Glu 165 170 175 Asp Lys Leu Ile Ala Ala Leu Asp Pro Asn Gly Phe Arg Pro Leu Ser 180 185 190 Ile Gly Lys Met Ala Asn Gly Ala Val Val Val Ser Ser Glu Thr Cys 195 200 205 Ala Phe Glu Val Ile Gly Ala Glu Trp Ile Arg Asp Leu Lys Pro Gly 210 215 220 Glu Ile Val Ile Ile Asp Asp Glu Gly Ile Gln Tyr Asp Ser Tyr Thr 225 230 235 240 Asp Asp Thr Gln Leu Ala Val Cys Ser Met Glu Tyr Ile Tyr Phe Ala 245 250 255 Arg Pro Asp Ser Asn Ile His Gly Val Asn Val His Thr Ala Arg Lys 260 265 270 Arg Met Gly Ala Gln Leu Ala Arg Glu Phe Lys His Glu Ala Asp Ile 275 280 285 Val Val Gly Val Pro Asn Ser Ser Leu Ser Ala Ala Met Gly Phe Ala 290 295 300 Glu Glu Ala Gly Leu Pro Asn Glu Met Gly Leu Ile Lys Asn Gln Tyr 305 310 315 320 Thr Gln Arg Thr Phe Ile Gln Pro Thr Gln Glu Leu Arg Glu Gln Gly 325 330 335 Val Arg Met Lys Leu Ser Ala Val Ser Gly Val Val Lys Gly Lys Arg 340 345 350 Val Val Met Val Asp Asp Ser Ile Val Arg Gly Thr Thr Ser Arg Arg 355 360 365 Ile Val Gln Leu Leu Lys Glu Ala Gly Ala Thr Glu Val His Val Ala 370 375 380 Ile Gly Ser Pro Ala Leu Ala Tyr Pro Cys Phe Tyr Gly Ile Asp Ile 385 390 395 400 Gln Thr Arg Gln Glu Leu Ile Ala Ala Asn His Thr Val Val Glu Glu Thr 405 410 415 Arg Gln Ile Ile Gly Ala Asp Ser Met Thr Tyr Leu Ser Ile Asp Gly 420 425 430 Leu Ile Glu Ser Ile Gly Ile Glu Thr Asp Ala Pro Asn Gly Gly Leu 435 440 445 Cys Val Ala Tyr Phe Asp Gly Asp Tyr Pro Thr Pro Leu Tyr Asp Tyr 450 455 460 Glu Glu Asp Tyr Arg Arg Ser Leu Glu Glu Lys Thr Ser Phe Tyr Lys 465 470 475 480 <210> 5 <211> 957 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (957) <223> Streptococcus pneumoniae spr0081 gene <400> 5 atggatatca aaataaaaag ggaggaaatt atgaaaaagt tttcaaaaac attgagagac 60 aactggatct ttctcttgat ggttttgcca ggggcactct ggttgattct attcttttac 120 attccagtat ttgggaacgt ggttgccttc aaagactacc acatgaccag taatggtttc 180 atagatagta tcataaatag taaatgggtc ggactcgata atttcagatt cttatttagt 240 tcaagagacg cctttattat cacacgaaat actgtcctct acaatcttgg ctttatcttt 300 ctaggtttag ttgtatctgt agggattgcc attatcctca gcgagctccg ttctaagaga 360 atggtgaaga tttttcaaac ttctatgttg ttcccttact tcttgtcttg ggttatcatt 420 agtttcttta cagatgcctt cctaaatatt gataaagggg tgttcaatca tctattggaa 480 agtcttggtc tcaaagaagt caatttctac gctgacctgg gcatctggcc ctatctccta 540 cttttcctag gtatttggaa aggctttgga tatagcagtg tcatgtacta tgcgacgatc 600 atgggaattg atccaaccta ctacgaagca gcgacagtgg acggagctag caagtggcaa 660 cgtattcgca acgtaaccat tcctcagttg actccgcttg taactgtatt gaccatcctt 720 gcagtcggaa atatcttccg cgcagacttc ggtctcttct atcaaatccc acacaatgct 780 ggtcagcttt acaatgtaac caacgttttg gacgtatatg tctttaatgg tttgactcag 840 acagcagata tcggtatggc tgcagcagcc ggtctttacc aatccgttgt tggtttgatt 900 ctggttatcc tatcaaactt gcttgcaaga cgagtcgatc caaactcagc tttgttc 957 <210> 6 <211> 319 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (319) <223> ABC transporter membrane-spanning permease <400> 6 Met Asp Ile Lys Ile Lys Arg Glu Glu Ile Met Lys Lys Phe Ser Lys 1 5 10 15 Thr Leu Arg Asp Asn Trp Ile Phe Leu Leu Met Val Leu Pro Gly Ala 20 25 30 Leu Trp Leu Ile Leu Phe Phe Tyr Ile Pro Val Phe Gly Asn Val Val 35 40 45 Ala Phe Lys Asp Tyr His Met Thr Ser Asn Gly Phe Ile Asp Ser Ile 50 55 60 Ile Asn Ser Lys Trp Val Gly Leu Asp Asn Phe Arg Phe Leu Phe Ser 65 70 75 80 Ser Arg Asp Ala Phe Ile Ile Thr Arg Asn Thr Val Leu Tyr Asn Leu 85 90 95 Gly Phe Ile Phe Leu Gly Leu Val Val Ser Val Gly Ile Ala Ile Ile 100 105 110 Leu Ser Glu Leu Arg Ser Lys Arg Met Val Lys Ile Phe Gln Thr Ser 115 120 125 Met Leu Phe Pro Tyr Phe Leu Ser Trp Val Ile Ile Ser Phe Phe Thr 130 135 140 Asp Ala Phe Leu Asn Ile Asp Lys Gly Val Phe Asn His Leu Leu Glu 145 150 155 160 Ser Leu Gly Leu Lys Glu Val Asn Phe Tyr Ala Asp Leu Gly Ile Trp 165 170 175 Pro Tyr Leu Leu Leu Phe Leu Gly Ile Trp Lys Gly Phe Gly Tyr Ser 180 185 190 Ser Val Met Tyr Tyr Ala Thr Ile Met Gly Ile Asp Pro Thr Tyr Tyr 195 200 205 Glu Ala Ala Thr Val Asp Gly Ala Ser Lys Trp Gln Arg Ile Arg Asn 210 215 220 Val Thr Ile Pro Gln Leu Thr Pro Leu Val Thr Val Leu Thr Ile Leu 225 230 235 240 Ala Val Gly Asn Ile Phe Arg Ala Asp Phe Gly Leu Phe Tyr Gln Ile 245 250 255 Pro His Asn Ala Gly Gln Leu Tyr Asn Val Thr Asn Val Leu Asp Val 260 265 270 Tyr Val Phe Asn Gly Leu Thr Gln Thr Ala Asp Ile Gly Met Ala Ala 275 280 285 Ala Ala Gly Leu Tyr Gln Ser Val Val Gly Leu Ile Leu Val Ile Leu 290 295 300 Ser Asn Leu Leu Ala Arg Arg Val Asp Pro Asn Ser Ala Leu Phe 305 310 315 <210> 7 <211> 681 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene <222> (1) .. (681) <223> Streptococcus pneumoniae spr0129 gene <400> 7 atgaaagtat tagcttttga tacgtccagc aaggctcttt ctctcgctat tttagaggat 60 aagcaggttc ttgccgagac gacgattaat attaagaaaa atcacagtat tacccttatg 120 cctgccatag attttttgat ggcaagtttg gattggacac ccaaggactt ggaccgaatc 180 gtggtagctg aagggccggg tagctataca ggcttgcgaa ttgcggtagc aactgctaag 240 accttagctc acaccctgaa catcgagttg gttggtatgt cgagtctctt ggctctggtg 300 ccctaccaac aagaaggttt gtttatcccc ttgatggatg cgcgtcgcaa taatgtttat 360 gcaggatttt atgaaaatgc caaacctgtc atggcagaag cacatctgcc ttttgagcga 420 gtcattgagc taatcaaggg cgctagtcag gtaacctttg tcggagaagt tggccccttt 480 gttgagcaga ttcaaaaaca cttgccaagg actgattaca aagaaacatt gcccaatgca 540 gctaatctag ctcttttggc ctgggacaag gaagcagact ccttgcatga ttttgtgcca 600 aattacctca aacgtgttga ggcagaggaa aactggctca agaaccatac cgagtctggt 660 gagtcctaca ttaaacgcct a 681 <210> 8 <211> 227 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (227) <223> Conserved hypothetical protein <400> 8 Met Lys Val Leu Ala Phe Asp Thr Ser Ser Lys Ala Leu Ser Leu Ala 1 5 10 15 Ile Leu Glu Asp Lys Gln Val Leu Ala Glu Thr Thr Ile Asn Ile Lys 20 25 30 Lys Asn His Ser Ile Thr Leu Met Pro Ala Ile Asp Phe Leu Met Ala 35 40 45 Ser Leu Asp Trp Thr Pro Lys Asp Leu Asp Arg Ile Val Val Ala Glu 50 55 60 Gly Pro Gly Ser Tyr Thr Gly Leu Arg Ile Ala Val Ala Thr Ala Lys 65 70 75 80 Thr Leu Ala His Thr Leu Asn Ile Glu Leu Val Gly Met Ser Ser Leu 85 90 95 Leu Ala Leu Val Pro Tyr Gln Gln Glu Gly Leu Phe Ile Pro Leu Met 100 105 110 Asp Ala Arg Arg Asn Asn Val Tyr Ala Gly Phe Tyr Glu Asn Ala Lys 115 120 125 Pro Val Met Ala Glu Ala His Leu Pro Phe Glu Arg Val Ile Glu Leu 130 135 140 Ile Lys Gly Ala Ser Gln Val Thr Phe Val Gly Glu Val Gly Pro Phe 145 150 155 160 Val Glu Gln Ile Gln Lys His Leu Pro Arg Thr Asp Tyr Lys Glu Thr 165 170 175 Leu Pro Asn Ala Ala Asn Leu Ala Leu Leu Ala Trp Asp Lys Glu Ala 180 185 190 Asp Ser Leu His Asp Phe Val Pro Asn Tyr Leu Lys Arg Val Glu Ala 195 200 205 Glu Glu Asn Trp Leu Lys Asn His Thr Glu Ser Gly Glu Ser Tyr Ile 210 215 220 Lys Arg Leu 225 <210> 9 <211> 1008 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1008) <223> Streptococcus pneumoniae spr0131 gene <400> 9 atgaaggata gatatatttt agcatttgag acatcctgtg atgagaccag tgtcgccgtc 60 ttgaaaaacg acgatgagct cttgtccaat gtcattgcta gtcaaattga gagtcacaaa 120 cgttttggtg gcgtagtgcc cgaagtagcc agtcgtcacc atgtcgaggt cattacagcc 180 tgtatcgagg aggcattggc agaagcaggg attaccgaag aggacgtgac agctgttgcg 240 gttacctacg gaccaggctt ggtcggagcc ttgctagttg gtttgtcagc tgccaaggcc 300 tttgcttggg ctcacggact tccactgatt cctgttaatc acatggctgg gcacctcatg 360 gcagctcaga gtgtggagcc tttggagttt cccttgctag cccttttagt cagtggtggg 420 cacacagagt tggtctatgt ttctgaggct ggcgattaca agattgttgg ggagacacga 480 gacgatgcag ttggggaggc ttatgacaag gtcggtcgtg tcatgggctt gacctatcct 540 gcaggtcgtg agattgacga gctggctcat caggggcagg atatttatga tttcccccgt 600 gccatgatta aggaagataa tctggagttc tccttctcag gtttgaaatc tgcctttatc 660 aatcttcatc acaatgccga gcaaaaggga gaaagcctgt ctacagaaga tttgtgtgct 720 tccttccaag cagcagttat ggacattctc atggcaaaaa ccaagaaggc tttggagaaa 780 tatcctgtta aaaccctagt tgtggcaggt ggtgtggcag ccaataaagg tctcagagaa 840 cgcctagcaa ctgaaatcac agatgtcaat gttatcattc cacctctgcg tctctgcgga 900 gacaatgcag gtatgattgc ttatgccagt gtcagcgagt ggaacaaaga aaactttgca 960 aacttggacc tcaatgccaa accaagtctt gcctttgata ccatggaa 1008 <210> 10 <211> 336 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (336) <223> Secreted metalloendopeptidase Gcp <400> 10 Met Lys Asp Arg Tyr Ile Leu Ala Phe Glu Thr Ser Cys Asp Glu Thr 1 5 10 15 Ser Val Ala Val Leu Lys Asn Asp Asp Glu Leu Leu Ser Asn Val Ile 20 25 30 Ala Ser Gln Ile Glu Ser His Lys Arg Phe Gly Gly Val Val Pro Glu 35 40 45 Val Ala Ser Arg His His Val Glu Val Ile Thr Ala Cys Ile Glu Glu 50 55 60 Ala Leu Ala Glu Ala Gly Ile Thr Glu Glu Asp Val Thr Ala Val Ala 65 70 75 80 Val Thr Tyr Gly Pro Gly Leu Val Gly Ala Leu Leu Val Gly Leu Ser 85 90 95 Ala Ala Lys Ala Phe Ala Trp Ala His Gly Leu Pro Leu Ile Pro Val 100 105 110 Asn His Met Ala Gly His Leu Met Ala Ala Gln Ser Val Glu Pro Leu 115 120 125 Glu Phe Pro Leu Leu Ala Leu Leu Val Ser Gly Gly His Thr Glu Leu 130 135 140 Val Tyr Val Ser Glu Ala Gly Asp Tyr Lys Ile Val Gly Glu Thr Arg 145 150 155 160 Asp Asp Ala Val Gly Glu Ala Tyr Asp Lys Val Gly Arg Val Met Gly 165 170 175 Leu Thr Tyr Pro Ala Gly Arg Glu Ile Asp Glu Leu Ala His Gln Gly 180 185 190 Gln Asp Ile Tyr Asp Phe Pro Arg Ala Met Ile Lys Glu Asp Asn Leu 195 200 205 Glu Phe Ser Phe Ser Gly Leu Lys Ser Ala Phe Ile Asn Leu His His 210 215 220 Asn Ala Glu Gln Lys Gly Glu Ser Leu Ser Thr Glu Asp Leu Cys Ala 225 230 235 240 Ser Phe Gln Ala Ala Val Met Asp Ile Leu Met Ala Lys Thr Lys Lys 245 250 255 Ala Leu Glu Lys Tyr Pro Val Lys Thr Leu Val Val Ala Gly Gly Val 260 265 270 Ala Ala Asn Lys Gly Leu Arg Glu Arg Leu Ala Thr Glu Ile Thr Asp 275 280 285 Val Asn Val Ile Ile Pro Pro Leu Arg Leu Cys Gly Asp Asn Ala Gly 290 295 300 Met Ile Ala Tyr Ala Ser Val Ser Glu Trp Asn Lys Glu Asn Phe Ala 305 310 315 320 Asn Leu Asp Leu Asn Ala Lys Pro Ser Leu Ala Phe Asp Thr Met Glu 325 330 335 <210> 11 <211> 1170 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1170) <223> Streptococcus pneumoniae spr0139 gene <400> 11 atgaatatag cagtaattgg tttggggcat gttgggctgg cctacgcgtt actatttgca 60 tctaaatata aagttgttgc atatgatata gattctgtaa aaataaataa tttaaaaaag 120 ggcattcttc catctaaaaa tgaagagctt atgaagtttt tttgcgagaa taacttaaat 180 attacttttt ttgatacatt ttctgaaatt aaaaataata ttgattatta tattattgcg 240 cttccgacag attatgatga gaaaattggt agttttaata catatgaaat cgaacaaacg 300 gtatcgaaga ttctgagggt aaaacctaat ggaaagatta ttttaaagtc aacagttccg 360 atcggctttt caaacaaatt aaaaaggctg tttgatacaa aaaatatcat ttttgtccct 420 gaatttttga gagaaggttg ttctatatat gataatttat atccaagtcg catagttgtt 480 ggagatgaga cagttgaagg aagaaaaatt gcagagttgt tcctttcgat tagtactcat 540 agtactgcca atattaaaaa tgttatgtta gtttctccta ctgaagcaga agcaattaag 600 cttttttcta acacattctt agctctccgt gttgcttttt ttaatgaact agattctttt 660 gctgagagga gaagtttaaa tgctgaagtt gtaataaaag gtgtttgttt agatccaaga 720 attggaaatt tttataataa tccttctttt ggatttggag gatattgtct tcccaaagat 780 actaaacaat taaaaaaaga atttatagaa ataaatgccc cagtgataga agcgattgat 840 atttcaaaca caaatagaaa acagttcata gttaaacaaa tattggaacg aaagccaaag 900 atagtaggaa tatataaatt ggggatgaaa tataattcag ataattacaa agagtcagct 960 attttaagta taattaatga actattgatt gtcggtataa aaattttagt atatgaacca 1020 aatttaaacg ttagtataga taacgtaatt tttgaaaaga attttgaact atttacaaaa 1080 caaagtgatc taattgttgc aaatagatgg gataggggcc ttgaagctta taaagataaa 1140 gtttacacaa gggggatctg gataagagat 1170 <210> 12 <211> 390 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (390) UDP-glucose dehydrogenase <400> 12 Met Asn Ile Ala Val Ile Gly Leu Gly His Val Gly Leu Ala Tyr Ala 1 5 10 15 Leu Leu Phe Ala Ser Lys Tyr Lys Val Val Ala Tyr Asp Ile Asp Ser 20 25 30 Val Lys Ile Asn Asn Leu Lys Lys Gly Ile Leu Pro Ser Lys Asn Glu 35 40 45 Glu Leu Met Lys Phe Phe Cys Glu Asn Asn Leu Asn Ile Thr Phe Phe 50 55 60 Asp Thr Phe Ser Glu Ile Lys Asn Asn Ile Asp Tyr Tyr Ile Ile Ala 65 70 75 80 Leu Pro Thr Asp Tyr Asp Glu Lys Ile Gly Ser Phe Asn Thr Tyr Glu 85 90 95 Ile Glu Gln Thr Val Ser Lys Ile Leu Arg Val Lys Pro Asn Gly Lys 100 105 110 Ile Ile Leu Lys Ser Thr Val Pro Ile Gly Phe Ser Asn Lys Leu Lys 115 120 125 Arg Leu Phe Asp Thr Lys Asn Ile Ile Phe Val Pro Glu Phe Leu Arg 130 135 140 Glu Gly Cys Ser Ile Tyr Asp Asn Leu Tyr Pro Ser Arg Ile Val Val 145 150 155 160 Gly Asp Glu Thr Val Glu Gly Arg Lys Ile Ala Glu Leu Phe Leu Ser 165 170 175 Ile Ser Thr His Ser Thr Ala Asn Ile Lys Asn Val Met Leu Val Ser 180 185 190 Pro Thr Glu Ala Glu Ala Ile Lys Leu Phe Ser Asn Thr Phe Leu Ala 195 200 205 Leu Arg Val Ala Phe Phe Asn Glu Leu Asp Ser Phe Ala Glu Arg Arg 210 215 220 Ser Leu Asn Ala Glu Val Val Ile Lys Gly Val Cys Leu Asp Pro Arg 225 230 235 240 Ile Gly Asn Phe Tyr Asn Asn Pro Ser Phe Gly Phe Gly Gly Tyr Cys 245 250 255 Leu Pro Lys Asp Thr Lys Gln Leu Lys Lys Glu Phe Ile Glu Ile Asn 260 265 270 Ala Pro Val Ile Glu Ala Ile Asp Ile Ser Asn Thr Asn Arg Lys Gln 275 280 285 Phe Ile Val Lys Gln Ile Leu Glu Arg Lys Pro Lys Ile Val Gly Ile 290 295 300 Tyr Lys Leu Gly Met Lys Tyr Asn Ser Asp Asn Tyr Lys Glu Ser Ala 305 310 315 320 Ile Leu Ser Ile Ile Asn Glu Leu Leu Ile Val Gly Ile Lys Ile Leu 325 330 335 Val Tyr Glu Pro Asn Leu Asn Val Ser Ile Asp Asn Val Ile Phe Glu 340 345 350 Lys Asn Phe Glu Leu Phe Thr Lys Gln Ser Asp Leu Ile Val Ala Asn 355 360 365 Arg Trp Asp Arg Gly Leu Glu Ala Tyr Lys Asp Lys Val Tyr Thr Arg 370 375 380 Gly Ile Trp Ile Arg Asp 385 390 <210> 13 <211> 594 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (594) <223> Streptococcus pneumoniae spr0145 gene <400> 13 atgctatggc tggattcttt tcagtctact tatactcttc gaaactctct tcaaaccacg 60 tcagcttcac cttgccgtag tacggttact gacttcgtca gttctatcca caacctcaaa 120 acggtggttc aagctcctgt ggcagctatt gctatgacgg tttttctaat caacttgcgt 180 ctctttttgt tgagtttaca cgcatcgact tatttccgtc ataccagtct ttggtacaat 240 atcggtatgt ctagtatctt gacagatgag acctatggcg ttttgatggg tgaattggcc 300 catacagaca aggtaaatcc tatgtggatg cacggaaaca atcttaacag ctatgtggct 360 tggtttgtgg ggacagtagt cggaacggct ctgggtggcc tgctaccaaa tccagaaatc 420 tttgccttga ttctttcaag cgtagtgaca ggtaaggttg ggagccttcc tcaaattaaa 480 tggctagact tcttagccgt ctttccaaca gcttgggtag cctttcgcta ccgcaatcta 540 gtcggaacag ttctctttgg agtggtcttg attgccatct tgcgtttggt cttt 594 <210> 14 <211> 198 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (198) <223> Conserved hypothetical protein <400> 14 Met Leu Trp Leu Asp Ser Phe Gln Ser Thr Tyr Thr Leu Arg Asn Ser 1 5 10 15 Leu Gln Thr Thr Ser Ala Ser Pro Cys Arg Ser Thr Val Thr Asp Phe 20 25 30 Val Ser Ser Ile His Asn Leu Lys Thr Val Val Gln Ala Pro Val Ala 35 40 45 Ala Ile Ala Met Thr Val Phe Leu Ile Asn Leu Arg Leu Phe Leu Leu 50 55 60 Ser Leu His Ala Ser Thr Tyr Phe Arg His Thr Ser Leu Trp Tyr Asn 65 70 75 80 Ile Gly Met Ser Ser Ile Leu Thr Asp Glu Thr Tyr Gly Val Leu Met 85 90 95 Gly Glu Leu Ala His Thr Asp Lys Val Asn Pro Met Trp Met His Gly 100 105 110 Asn Asn Leu Asn Ser Tyr Val Ala Trp Phe Val Gly Thr Val Val Gly 115 120 125 Thr Ala Leu Gly Gly Leu Leu Pro Asn Pro Glu Ile Phe Ala Leu Ile 130 135 140 Leu Ser Ser Val Val Thr Gly Lys Val Gly Ser Leu Pro Gln Ile Lys 145 150 155 160 Trp Leu Asp Phe Leu Ala Val Phe Pro Thr Ala Trp Val Ala Phe Arg 165 170 175 Tyr Arg Asn Leu Val Gly Thr Val Leu Phe Gly Val Val Leu Ile Ala 180 185 190 Ile Leu Arg Leu Val Phe 195 <210> 15 <211> 600 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (600) <223> Streptococcus pneumoniae spr0156 gene <400> 15 gtggcgacaa cagaaagcct tggcagaaga agaggaaaca gaagagctta cctaagtatt 60 gacaaaaaag aactatcaag atacaatctt ggtagttgtt ttttgattat agataaaatc 120 atggaggtac atatgaagac aatttcttta gtttatatta gtttgagtgg caacactgaa 180 agttttgtga cgcgcttgaa agactatctc ttgtcccagt acaaaaggat tgaggttcaa 240 aagattcata tcaaggattt ggtcaaggaa ggcaaaaatt tctatgaaat ggaccatccc 300 tatgtcgctt ttttgccgac ctatctcgaa ggtgggaatg gcgtggataa cggagatgtt 360 gagattttga cgacaccggt gggagatttt attgcctatg gtaacaatgc tagtaagtgt 420 tttggtgtgg ttgggtcagg aaatcgtaac tttaataacc aatactgcct aacagccaag 480 caatacagtc aacgctttgg cttccctgta ttggctgact ttgaaatgcg aggcatgctg 540 gaagatatca aacatgtcgc agctattatc gcagatttgt atgagttgga aaaggagaat 600 600 <210> 16 <211> 200 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (200) <223> Conserved hypothetical protein <400> 16 Met Ala Thr Thr Glu Ser Leu Gly Arg Arg Arg Gly Asn Arg Arg Ala 1 5 10 15 Tyr Leu Ser Ile Asp Lys Lys Glu Leu Ser Arg Tyr Asn Leu Gly Ser 20 25 30 Cys Phe Leu Ile Ile Asp Lys Ile Met Glu Val His Met Lys Thr Ile 35 40 45 Ser Leu Val Tyr Ile Ser Leu Ser Gly Asn Thr Glu Ser Phe Val Thr 50 55 60 Arg Leu Lys Asp Tyr Leu Leu Ser Gln Tyr Lys Arg Ile Glu Val Gln 65 70 75 80 Lys Ile His Ile Lys Asp Leu Val Lys Glu Gly Lys Asn Phe Tyr Glu 85 90 95 Met Asp His Pro Tyr Val Ala Phe Leu Pro Thr Tyr Leu Glu Gly Gly 100 105 110 Asn Gly Val Asp Asn Gly Asp Val Glu Ile Leu Thr Thr Pro Val Gly 115 120 125 Asp Phe Ile Ala Tyr Gly Asn Asn Ala Ser Lys Cys Phe Gly Val Val 130 135 140 Gly Ser Gly Asn Arg Asn Phe Asn Asn Gln Tyr Cys Leu Thr Ala Lys 145 150 155 160 Gln Tyr Ser Gln Arg Phe Gly Phe Pro Val Leu Ala Asp Phe Glu Met 165 170 175 Arg Gly Met Leu Glu Asp Ile Lys His Val Ala Ala Ile Ile Ala Asp 180 185 190 Leu Tyr Glu Leu Glu Lys Glu Asn 195 200 <210> 17 <211> 396 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (396) <223> Streptococcus pneumoniae spr0173 gene <400> 17 atgattaaaa tttatacagt ctcaagttgt actagctgta aaaaagcaaa aacctggctc 60 aatgcccacc agttaagtta taaagaacaa aaccttggta aagaaggaat tacgagagaa 120 gaattactgg atattctaac caaaacagat aacggaatag ccagcatcgt ttcgtctaaa 180 aatcgctatg ccaaagccct tggagtggat attgaagatt tgagtgtcaa tgaagttctc 240 aatctgatta tggaaacacc gagaatttta aagagcccaa tccttgtaga tgaaaaacgc 300 ctgcaagttg gctacaagga agacgatatt cgtgccttcc taccacgctc tgtccgtaat 360 gtagaaaatg cagaagcacg tttgcgtgca gctcta 396 <210> 18 <211> 132 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN <222> (1) .. (132) <223> Arsenate reductase <400> 18 Met Ile Lys Ile Tyr Thr Val Ser Ser Cys Thr Ser Cys Lys Lys Ala 1 5 10 15 Lys Thr Trp Leu Asn Ala His Gln Leu Ser Tyr Lys Glu Gln Asn Leu 20 25 30 Gly Lys Glu Gly Ile Thr Arg Glu Glu Leu Leu Asp Ile Leu Thr Lys 35 40 45 Thr Asp Asn Gly Ile Ala Ser Ile Val Ser Ser Lys Asn Arg Tyr Ala 50 55 60 Lys Ala Leu Gly Val Asp Ile Glu Asp Leu Ser Val Asn Glu Val Leu 65 70 75 80 Asn Leu Ile Met Glu Thr Pro Arg Ile Leu Lys Ser Pro Ile Leu Val 85 90 95 Asp Glu Lys Arg Leu Gln Val Gly Tyr Lys Glu Asp Asp Ile Arg Ala 100 105 110 Phe Leu Pro Arg Ser Val Arg Asn Val Glu Asn Ala Glu Ala Arg Leu 115 120 125 Arg Ala Ala Leu 130 <210> 19 <211> 636 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene <222> (1) .. (636) <223> Streptococcus pneumoniae spr0210 gene <400> 19 atgaatcttt tgattatggg cttacctggt gcaggtaagg gaactcaagc agcaaaaatc 60 gtagaacaat tccatgttgc acatatctca acaggtgata tgttccgcgc tgcaatggca 120 aatcaaactg aaatgggtgt tcttgctaag tcatatattg acaagggtga attggttcct 180 gacgaagtta caaatggaat cgtaaaagaa cgcctttcac aagatgatat taaagaaaca 240 ggattcttat tggatggtta cccacgtaca attgaacaag ctcatgcctt ggacaaaaca 300 ttggctgaac ttggcattga actagaaggt attatcaata ttgaagtgaa ccctgacagc 360 ctcttggaac gtttgagtgg ccgtatcatc caccgcgtaa ctggagaaac tttccacaag 420 gtctttaacc caccagttga ctataaagaa gaagattact accaacgtga agatgataag 480 cctgagacag taaaacgtcg tttggatgtt aatattgctc aaggagaacc aatcattgct 540 cactaccgtg ccaaaggttt ggttcatgac atcgaaggta atcaagatat caatgatgtc 600 ttctcagata ttgaaaaagt attgacaaat ttgaaa 636 <210> 20 <211> 212 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (212) Adenylate kinase (ATP-AMP transphosphorylase) <400> 20 Met Asn Leu Leu Ile Met Gly Leu Pro Gly Ala Gly Lys Gly Thr Gln 1 5 10 15 Ala Ala Lys Ile Val Glu Gln Phe His Val Ala His Ile Ser Thr Gly 20 25 30 Asp Met Phe Arg Ala Ala Met Ala Asn Gln Thr Glu Met Gly Val Leu 35 40 45 Ala Lys Ser Tyr Ile Asp Lys Gly Glu Leu Val Pro Asp Glu Val Thr 50 55 60 Asn Gly Ile Val Lys Glu Arg Leu Ser Gln Asp Asp Ile Lys Glu Thr 65 70 75 80 Gly Phe Leu Leu Asp Gly Tyr Pro Arg Thr Ile Glu Gln Ala His Ala 85 90 95 Leu Asp Lys Thr Leu Ala Glu Leu Gly Ile Glu Leu Glu Gly Ile Ile 100 105 110 Asn Ile Glu Val Asn Pro Asp Ser Leu Leu Glu Arg Leu Ser Gly Arg 115 120 125 Ile Ile His Arg Val Thr Gly Glu Thr Phe His Lys Val Phe Asn Pro 130 135 140 Pro Val Asp Tyr Lys Glu Glu Asp Tyr Tyr Gln Arg Glu Asp Asp Lys 145 150 155 160 Pro Glu Thr Val Lys Arg Arg Leu Asp Val Asn Ile Ala Gln Gly Glu 165 170 175 Pro Ile Ile Ala His Tyr Arg Ala Lys Gly Leu Val His Asp Ile Glu 180 185 190 Gly Asn Gln Asp Ile Asn Asp Val Phe Ser Asp Ile Glu Lys Val Leu 195 200 205 Thr Asn Leu Lys 210 <210> 21 <211> 1851 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) ... (1851) <223> Streptococcus pneumoniae spr0243 gene <400> 21 atgaaacaaa gtaaaatgcc tatcccaacg cttcgcgaaa tgccaagcga tgctcaagtt 60 atcagccatg ctcttatgtt gcgtgctggt tatgttcgcc aagtttcagc aggtgtttat 120 tcttatctac cacttgccaa ccgtgtgatt gaaaaagcta aaaacatcat gcgccaagaa 180 ttcgaaaaga ttggtgctgt tgagatgttg gctccagccc ttcttagtgc agaattgtgg 240 cgtgaatcag gtcgttacga aacctatggt gaagaccttt acaaactaaa aaaccgtgaa 300 aaatcagact ttatcttagg tccaactcac gaagaaacct ttacagctat tgtccgtgat 360 tctgttaaat cttacaagca attgccactc aacctttatc aaattcagcc caagtatcgt 420 gatgaaaaac gcccgcgtaa tggacttctt cgtacacgtg agtttatcat gaaggatgct 480 tatagtttcc acgctaacta tgatagtttg gatagtgttt atgatgagta caaagcagcc 540 tatgagcgta ttttcactcg tagtggttta gacttcaagg ctattattgg tgacggtgga 600 gccatgggtg gtaaggatag ccaagaattt atggccatta catctgctcg tacagacctt 660 gaccgctggg ttgtcttgga caagtcagtt gtctcatttg acgaaattcc tgtagaagtg 720 caagaagaaa tcaaggcaga attgctcaaa tggatagtct ctggtgaaga taccattgct 780 tactcaagtg agtctagcta tgcagctaac ttagaaatgg caacaaacga gtacaaacca 840 agcaaccgtg ttgtcgctga agaagaagtt actcgtgttg caacgccaga tgttaaatca 900 attgatgaag ttgcagcctt cctcaatgtt ccagaagaac aaacgattaa aaccctcttc 960 tacattgcag atggtgagct tgttgcagcc cttctagttg gaaatgacca actcaacgaa 1020 gtcaagttga aaaatcactt gggagcagat ttctttgacg ttgctagcga agaagaagtg 1080 gcgaatgttg ttcaagcagg atttggttca cttggaccag ttggtttgcc agagaatatt 1140 aaaattattg cagatcgtaa ggtacaagat gttcgcaatg cagttgtcgg tgctaacgaa 1200 gatggctacc acttgactgg tgtgaaccca ggccgtgatt ttactgcaga atatgtggat 1260 atccgtgaag ttcgtgaggg tgaaatttcc ccagatggac aaggtgtcct taactttgcg 1320 cgtggtattg agatcggtca tattttcaaa ctcggaactc gctattcagc aagcatggga 1380 gcagatgtct tggatgaaaa tggtcgtgct gtgccaatca tcatgggatg ttacggtatc 1440 ggtgtcagcc gtcttctttc agcagtgatg gagcaacacg ctcgcctctt tgttaacaaa 1500 acgccaaaag gtgaataccg ttacgcttgg ggaatcaatt tccctaaaga attggcacca 1560 tttgatgtgc atttgattac tgtcaatgtc aaggatgaag aagcgcaagc cttgacagaa 1620 aaacttgaag caagcttgat gggagctggt tatgaagtct tgacagatga ccgtaacgaa 1680 cgtgtcggtg ttaaatttag cgatagtgac ttgattggtc ttccaatccg tatcactgtt 1740 ggtaaaaaag cggccgatgg cattgtagaa gttaagatca aggcaactgg tgacaccatt 1800 gaggttcatg cagataacgt gcttgaaacg cttgaaatcc taagcaagaa a 1851 <210> 22 <211> 617 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (617) <223> Prolyl-tRNA synthetase <400> 22 Met Lys Gln Ser Lys Met Pro Ile Pro Thr Leu Arg Glu Met Pro Ser 1 5 10 15 Asp Ala Gln Val Ile Ser His Ala Leu Met Leu Arg Ala Gly Tyr Val 20 25 30 Arg Gln Val Ser Ala Gly Val Tyr Ser Tyr Leu Pro Leu Ala Asn Arg 35 40 45 Val Ile Glu Lys Ala Lys Asn Ile Met Arg Gln Glu Phe Glu Lys Ile 50 55 60 Gly Ala Val Glu Met Leu Ala Pro Ala Leu Leu Ser Ala Glu Leu Trp 65 70 75 80 Arg Glu Ser Gly Arg Tyr Glu Thr Tyr Gly Glu Asp Leu Tyr Lys Leu 85 90 95 Lys Asn Arg Glu Lys Ser Asp Phe Ile Leu Gly Pro Thr His Glu Glu 100 105 110 Thr Phe Thr Ala Ile Val Arg Asp Ser Val Lys Ser Tyr Lys Gln Leu 115 120 125 Pro Leu Asn Leu Tyr Gln Ile Gln Pro Lys Tyr Arg Asp Glu Lys Arg 130 135 140 Pro Arg Asn Gly Leu Leu Arg Thr Arg Glu Phe Ile Met Lys Asp Ala 145 150 155 160 Tyr Ser Phe His Ala Asn Tyr Asp Ser Leu Asp Ser Val Tyr Asp Glu 165 170 175 Tyr Lys Ala Ala Tyr Glu Arg Ile Phe Thr Arg Ser Gly Leu Asp Phe 180 185 190 Lys Ala Ile Ile Gly Asp Gly Gly Ala Met Gly Gly Lys Asp Ser Gln 195 200 205 Glu Phe Met Ala Ile Thr Ser Ala Arg Thr Asp Leu Asp Arg Trp Val 210 215 220 Val Leu Asp Lys Ser Val Val Ser Phe Asp Glu Ile Pro Val Glu Val 225 230 235 240 Gln Glu Glu Ile Lys Ala Glu Leu Leu Lys Trp Ile Val Ser Gly Glu 245 250 255 Asp Thr Ile Ala Tyr Ser Ser Glu Ser Ser Tyr Ala Ala Asn Leu Glu 260 265 270 Met Ala Thr Asn Glu Tyr Lys Pro Ser Asn Arg Val Val Ala Glu Glu 275 280 285 Glu Val Thr Arg Val Ala Thr Pro Asp Val Lys Ser Ile Asp Glu Val 290 295 300 Ala Ala Phe Leu Asn Val Pro Glu Glu Gln Thr Ile Lys Thr Leu Phe 305 310 315 320 Tyr Ile Ala Asp Gly Glu Leu Val Ala Ala Leu Leu Val Gly Asn Asp 325 330 335 Gln Leu Asn Glu Val Lys Leu Lys Asn His Leu Gly Ala Asp Phe Phe 340 345 350 Asp Val Ala Ser Glu Glu Glu Val Ala Asn Val Val Gln Ala Gly Phe 355 360 365 Gly Ser Leu Gly Pro Val Gly Leu Pro Glu Asn Ile Lys Ile Ile Ala 370 375 380 Asp Arg Lys Val Gln Asp Val Arg Asn Ala Val Val Gly Ala Asn Glu 385 390 395 400 Asp Gly Tyr His Leu Thr Gly Val Asn Pro Gly Arg Asp Phe Thr Ala 405 410 415 Glu Tyr Val Asp Ile Arg Glu Val Arg Glu Gly Glu Ile Ser Pro Asp 420 425 430 Gly Gln Gly Val Leu Asn Phe Ala Arg Gly Ile Glu Ile Gly His Ile 435 440 445 Phe Lys Leu Gly Thr Arg Tyr Ser Ala Ser Met Gly Ala Asp Val Leu 450 455 460 Asp Glu Asn Gly Arg Ala Val Pro Ile Ile Met Gly Cys Tyr Gly Ile 465 470 475 480 Gly Val Ser Arg Leu Leu Ser Ala Val Met Glu Gln His Ala Arg Leu 485 490 495 Phe Val Asn Lys Thr Pro Lys Gly Glu Tyr Arg Tyr Ala Trp Gly Ile 500 505 510 Asn Phe Pro Lys Glu Leu Ala Pro Phe Asp Val His Leu Ile Thr Val 515 520 525 Asn Val Lys Asp Glu Glu Ala Gln Ala Leu Thr Glu Lys Leu Glu Ala 530 535 540 Ser Leu Met Gly Ala Gly Tyr Glu Val Leu Thr Asp Asp Arg Asn Glu 545 550 555 560 Arg Val Gly Val Lys Phe Ser Asp Ser Asp Leu Ile Gly Leu Pro Ile 565 570 575 Arg Ile Thr Val Gly Lys Lys Ala Ala Asp Gly Ile Val Glu Val Lys 580 585 590 Ile Lys Ala Thr Gly Asp Thr Ile Glu Val His Ala Asp Asn Val Leu 595 600 605 Glu Thr Leu Glu Ile Leu Ser Lys Lys 610 615 <210> 23 <211> 942 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (942) <223> Streptococcus pneumoniae spr0266 gene <400> 23 atgtcaagta aagccaatca tgcaaagaca gttatttgcg gaattatcaa tgtaacccca 60 gactcctttt cggacggtgg tcaatttttt gctcttgagc aggcactcca gcaggctcgt 120 aaattgatag cagaaggagc cagtatgctc gatatcggcg gagaatcgac tcggccgggc 180 agtagctatg ttgagataga agaggaaatc cagcgtgttg ttccagtgat caaagcgatt 240 cgcaaggaaa gtgatgtcct catctctatt gatacttgga agagccaagt agcagaggct 300 gctttggctg ctggtgccga tctagtcaat gatatcactg gtcttatggg tgatgagaaa 360 atgcctcatg tggtagctga agcgagagcg caagtggtca tcatgtttaa tccagttatg 420 gcgcgacctc agcaccctag ctcgctcatc ttccctcatt ttggttttgg tcaagctttt 480 acagaggaag agttagctga ctttgaaaca ttgccaatcg aagaattgat ggaggctttc 540 tttgaacgag cactagcgag agcggcagaa gctggtattg caccagaaaa tatcctgttg 600 gatccaggaa ttggctttgg tctgaccaag aaagaaaatc tgcttctttt acgggacctg 660 gataaactac atcagaaggg ctatccaatc tttctcggag tgtcgcgcaa gcgatttgtc 720 atcaatatcc tagaggagaa tggttttgaa gtcaatcctg agacagagct tggtttccgc 780 aatcgggaca cggcttcggc tcatgtaacc agtatcgctg caagacaggg tgtagaagtg 840 gtgcgcgtgc atgacgtagc tagtcacagg atggcagttg aaattgcctc tgccattcgt 900 ctggctgatg aagcggaaaa tttagattta aaacaatata aa 942 <210> 24 <211> 314 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (314) <223> Dihydropteroate synthase <400> 24 Met Ser Ser Lys Ala Asn His Ala Lys Thr Val Ile Cys Gly Ile Ile 1 5 10 15 Asn Val Thr Pro Asp Ser Phe Ser Asp Gly Gly Gln Phe Phe Ala Leu 20 25 30 Glu Gln Ala Leu Gln Gln Ala Arg Lys Leu Ile Ala Glu Gly Ala Ser 35 40 45 Met Leu Asp Ile Gly Gly Glu Ser Thr Arg Pro Gly Ser Ser Tyr Val 50 55 60 Glu Ile Glu Glu Glu Ile Gln Arg Val Val Pro Val Ile Lys Ala Ile 65 70 75 80 Arg Lys Glu Ser Asp Val Leu Ile Ser Ile Asp Thr Trp Lys Ser Gln 85 90 95 Val Ala Glu Ala Ala Leu Ala Ala Gly Ala Asp Leu Val Asn Asp Ile 100 105 110 Thr Gly Leu Met Gly Asp Glu Lys Met Pro His Val Val Ala Glu Ala 115 120 125 Arg Ala Gln Val Val Ile Met Phe Asn Pro Val Met Ala Arg Pro Gln 130 135 140 His Pro Ser Ser Leu Ile Phe Pro His Phe Gly Phe Gly Gln Ala Phe 145 150 155 160 Thr Glu Glu Glu Leu Ala Asp Phe Glu Thr Leu Pro Ile Glu Glu Leu 165 170 175 Met Glu Ala Phe Phe Glu Arg Ala Leu Ala Arg Ala Ala Glu Ala Gly 180 185 190 Ile Ala Pro Glu Asn Ile Leu Leu Asp Pro Gly Ile Gly Phe Gly Leu 195 200 205 Thr Lys Lys Glu Asn Leu Leu Leu Leu Arg Asp Leu Asp Lys Leu His 210 215 220 Gln Lys Gly Tyr Pro Ile Phe Leu Gly Val Ser Arg Lys Arg Phe Val 225 230 235 240 Ile Asn Ile Leu Glu Glu Asn Gly Phe Glu Val Asn Pro Glu Thr Glu 245 250 255 Leu Gly Phe Arg Asn Arg Asp Thr Ala Ser Ala His Val Thr Ser Ile 260 265 270 Ala Ala Arg Gln Gly Val Glu Val Val Arg Val His Asp Val Ala Ser 275 280 285 His Arg Met Ala Val Glu Ile Ala Ser Ala Ile Arg Leu Ala Asp Glu 290 295 300 Ala Glu Asn Leu Asp Leu Lys Gln Tyr Lys 305 310 <210> 25 <211> 1320 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1320) <223> Streptococcus pneumoniae spr0267 gene <400> 25 atgaaagaaa ttgaaaacaa tcaatggatt gctaactacc ggacggatca accgcatttt 60 ggcttggaac gaatggtgga actgttagct ttgcgtggca atccccatct caaactcaag 120 gtcctccata tcggaggaac taacggcaag ggctcgacta ttgctttttt gaaaaagatg 180 ctagaaaagc tagggctgag agttggcgtg tttagctcgc cctatctcat tcattacaca 240 gaccagatta gcatcaatgg ggaatcgatc tcagaagcga ggctagaagc tctcatggca 300 gactatcagt ctttgctgga gggagaagcg gtcgccaatt tacagggcac aaccgagttt 360 gagattatca cagccctggc ctatgactac tttgcctcag agcaagtaga tgtggccatc 420 atggaagttg gcatgggtgg actttttgat agtaccaatg tctgtcagcc cattttgaca 480 ggaattacaa ctattggctt ggatcatgtg gccttacttg gtgacacctt ggaggccata 540 gcagagcaga aggcaggtat tatcaaacaa gggatgccct tggtaacagg gcgtattgct 600 ccagaagcct tggctgtgat tgaccgcatt gcggaaggga aagatgcgcc gagacttgcc 660 tacgggacag attatcaggt tcgtcatcaa gaaagtgtgg tgacagggga agtctttgac 720 tatacaagtg ctgtcagaca aggtcgcttc cagactagcc tgcttggttt gtaccaaata 780 gagaatgctg ggatggccat agctttactt gatacttttt gtcaagaaga tggtcgagag 840 ctagcaagca atgactttct tggtcaagcc ttggaagaaa caagttggcc agggcgtttg 900 gaaatcgtgt caagagatcc cttgatgatt ttggatggag cccataatcc ccatgctatc 960 aaggccttgt tggtaacctt gcaagaacgt tttgcggatt atcataagga aatcctcttc 1020 acttgtatca aaaccaaagc cttggaggat atgttggact tgctgggagc catgccagat 1080 accgagctta ctctaacaca ttttgcggat agtcgggcga cggatgaaag cgtgctgaaa 1140 gaggcagcta agtctagaaa tctcagctac caagattggc atgattttct agagcagaat 1200 gtgacagata aaaaagaaga gaaacaaaca gttaggattg tcacaggttc cttgtatttc 1260 ttgagccaag tgagggtcta tctgatggag aggaagaacg agaatggata cacaaaagat 1320 1320 <210> 26 <211> 440 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (440) <223> Dihydrofolate synthetase <400> 26 Met Lys Glu Ile Glu Asn Asn Gln Trp Ile Ala Asn Tyr Arg Thr Asp 1 5 10 15 Gln Pro His Phe Gly Leu Glu Arg Met Val Glu Leu Leu Ala Leu Arg 20 25 30 Gly Asn Pro His Leu Lys Leu Lys Val Leu His Ile Gly Gly Thr Asn 35 40 45 Gly Lys Gly Ser Thr Ile Ala Phe Leu Lys Lys Met Leu Glu Lys Leu 50 55 60 Gly Leu Arg Val Gly Val Phe Ser Ser Pro Tyr Leu Ile His Tyr Thr 65 70 75 80 Asp Gln Ile Ser Ile Asn Gly Glu Ser Ile Ser Glu Ala Arg Leu Glu 85 90 95 Ala Leu Met Ala Asp Tyr Gln Ser Leu Leu Glu Gly Glu Ala Val Ala 100 105 110 Asn Leu Gln Gly Thr Thr Glu Phe Glu Ile Ile Thr Ala Leu Ala Tyr 115 120 125 Asp Tyr Phe Ala Ser Glu Gln Val Asp Val Ala Ile Met Glu Val Gly 130 135 140 Met Gly Gly Leu Phe Asp Ser Thr Asn Val Cys Gln Pro Ile Leu Thr 145 150 155 160 Gly Ile Thr Thr Ile Gly Leu Asp His Val Ala Leu Leu Gly Asp Thr 165 170 175 Leu Glu Ala Ile Ala Glu Gln Lys Ala Gly Ile Ile Lys Gln Gly Met 180 185 190 Pro Leu Val Thr Gly Arg Ile Ala Pro Glu Ala Leu Ala Val Ile Asp 195 200 205 Arg Ile Ala Glu Gly Lys Asp Ala Pro Arg Leu Ala Tyr Gly Thr Asp 210 215 220 Tyr Gln Val Arg His Gln Glu Ser Val Val Thr Gly Glu Val Phe Asp 225 230 235 240 Tyr Thr Ser Ala Val Arg Gln Gly Arg Phe Gln Thr Ser Leu Leu Gly 245 250 255 Leu Tyr Gln Ile Glu Asn Ala Gly Met Ala Ile Ala Leu Leu Asp Thr 260 265 270 Phe Cys Gln Glu Asp Gly Arg Glu Leu Ala Ser Asn Asp Phe Leu Gly 275 280 285 Gln Ala Leu Glu Glu Thr Ser Trp Pro Gly Arg Leu Glu Ile Val Ser 290 295 300 Arg Asp Pro Leu Met Ile Leu Asp Gly Ala His Asn Pro His Ala Ile 305 310 315 320 Lys Ala Leu Leu Val Thr Leu Gln Glu Arg Phe Ala Asp Tyr His Lys 325 330 335 Glu Ile Leu Phe Thr Cys Ile Lys Thr Lys Ala Leu Glu Asp Met Leu 340 345 350 Asp Leu Leu Gly Ala Met Pro Asp Thr Glu Leu Thr Leu Thr His Phe 355 360 365 Ala Asp Ser Arg Ala Thr Asp Glu Ser Val Leu Lys Glu Ala Ala Lys 370 375 380 Ser Arg Asn Leu Ser Tyr Gln Asp Trp His Asp Phe Leu Glu Gln Asn 385 390 395 400 Val Thr Asp Lys Lys Glu Glu Lys Gln Thr Val Arg Ile Val Thr Gly 405 410 415 Ser Leu Tyr Phe Leu Ser Gln Val Arg Val Tyr Leu Met Glu Arg Lys 420 425 430 Asn Glu Asn Gly Tyr Thr Lys Asp 435 440 <210> 27 <211> 552 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (552) <223> Streptococcus pneumoniae spr0268 gene <400> 27 atggatacac aaaagattga agcagctgta aaaatgatta tcgaggctgt aggagaggac 60 gctaatcgcg agggtttgca ggaaacacct gctcgtgtag cccgtatgta tcaagagatt 120 ttttcaggtc ttggtcaaac agcagaggaa catttgtcaa aatcctttga aattattgac 180 gataatatgg tggtagaaaa ggatatcttt ttccatacca tgtgtgaaca ccacttcttg 240 ccattttatg gtagagcgca cattgcctac attccagatg gtcgtgtggc aggcttgtct 300 aagctagccc gtacggttga agtttattcg aaaaaaccac aaattcaaga acgtttgaat 360 atcgaagtgg ccgatgcctt gatggactat ctaggtgcta aaggagcctt tgttatcatt 420 gaggcggaac atatgtgtat gagtatgcgt ggtgttagaa aaccaggcac tgcaaccttg 480 acgacagtag ctcgtggtct atttgaaaca gataaggacc tccgcgacca agcttatcgt 540 ttaatggggc ta 552 <210> 28 <211> 184 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (184) GTP cyclohydrolase <400> 28 Met Asp Thr Gln Lys Ile Glu Ala Ala Val Lys Met Ile Ile Glu Ala 1 5 10 15 Val Gly Glu Asp Ala Asn Arg Glu Gly Leu Gln Glu Thr Pro Ala Arg 20 25 30 Val Ala Arg Met Tyr Gln Glu Ile Phe Ser Gly Leu Gly Gln Thr Ala 35 40 45 Glu Glu His Leu Ser Lys Ser Phe Glu Ile Ile Asp Asp Asn Met Val 50 55 60 Val Glu Lys Asp Ile Phe Phe His Thr Met Cys Glu His His Phe Leu 65 70 75 80 Pro Phe Tyr Gly Arg Ala His Ile Ala Tyr Ile Pro Asp Gly Arg Val 85 90 95 Ala Gly Leu Ser Lys Leu Ala Arg Thr Val Glu Val Tyr Ser Lys Lys 100 105 110 Pro Gln Ile Gln Glu Arg Leu Asn Ile Glu Val Ala Asp Ala Leu Met 115 120 125 Asp Tyr Leu Gly Ala Lys Gly Ala Phe Val Ile Glu Ala Glu His 130 135 140 Met Cys Met Ser Met Arg Gly Val Arg Lys Pro Gly Thr Ala Thr Leu 145 150 155 160 Thr Thr Val Ala Arg Gly Leu Phe Glu Thr Asp Lys Asp Leu Arg Asp 165 170 175 Gln Ala Tyr Arg Leu Met Gly Leu 180 <210> 29 <211> 810 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (810) <223> Streptococcus pneumoniae spr0269 gene <400> 29 atggatcaac tgcagattaa ggatttggaa atgtttgcct atcatggtct ttttcctagt 60 gagaaagaat tggggcagaa atttatcgtt tcagccatcc tatcctatga tatgaccaag 120 gcagctacag acttggattt aacagcctct gtccattacg gagaattgtg tcagcagtgg 180 acgacttggt ttcaggaaac gagtgaagat ttgattgaaa cggtagccta taaactggtg 240 gaacgtacct ttgagtctta tcctcttgtc caagaaatga agttggaact gaaaaaacct 300 tgggcaccgg tgcatttgtc actagatact tgctcggtaa ccattcatcg ccgcaagcaa 360 cgagccttta tcgccctagg aagcaatatg ggagataagc aagcaaactt gaagcaagcc 420 attgacaaac tgcgagctcg tggcatccat attctcaaag agtccagtgt cttagcgacg 480 gagccttggg gtggagtgga gcaggatagc tttgccaatc aagtggttga ggtggaaacc 540 tggctaccag cacaagactt gttagaaacc ttgttagcca ttgagtcaga gctgggacgg 600 gtgagagaag tgcattgggg acctcgtttg attgatttgg acttgctctt tgtggaggac 660 cagatccttt atacagacga cctcatattg cctcatcctt acatagcgga acgccttttt 720 gtccttgagt ccttacagga aattgcgcct cattttatcc atccgatatt aaaacaaccg 780 atccgcaact tgtatgatgc tttgaaaaaa 810 <210> 30 <211> 270 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (270) <223> Aldolase-pyrophosphokinase <400> 30 Met Asp Gln Leu Gln Ile Lys Asp Leu Glu Met Phe Ala Tyr His Gly 1 5 10 15 Leu Phe Pro Ser Glu Lys Glu Leu Gly Gln Lys Phe Ile Val Ser Ala 20 25 30 Ile Leu Ser Tyr Asp Met Thr Lys Ala Ala Thr Asp Leu Asp Leu Thr 35 40 45 Ala Ser Val His Tyr Gly Glu Leu Cys Gln Gln Trp Thr Thr Trp Phe 50 55 60 Gln Glu Thr Ser Glu Asp Leu Ile Glu Thr Val Ala Tyr Lys Leu Val 65 70 75 80 Glu Arg Thr Phe Glu Ser Tyr Pro Leu Val Gln Glu Met Lys Leu Glu 85 90 95 Leu Lys Lys Pro Trp Ala Pro Val His Leu Ser Leu Asp Thr Cys Ser 100 105 110 Val Thr Ile His Arg Arg Lys Gln Arg Ala Phe Ile Ala Leu Gly Ser 115 120 125 Asn Met Gly Asp Lys Gln Ala Asn Leu Lys Gln Ala Ile Asp Lys Leu 130 135 140 Arg Ala Arg Gly Ile His Ile Leu Lys Glu Ser Ser Val Leu Ala Thr 145 150 155 160 Glu Pro Trp Gly Gly Val Glu Gln Asp Ser Phe Ala Asn Gln Val Val 165 170 175 Glu Val Glu Thr Trp Leu Pro Ala Gln Asp Leu Leu Glu Thr Leu Leu 180 185 190 Ala Ile Glu Ser Glu Leu Gly Arg Val Arg Glu Val His Trp Gly Pro 195 200 205 Arg Leu Ile Asp Leu Asp Leu Leu Phe Val Glu Asp Gln Ile Leu Tyr 210 215 220 Thr Asp Asp Leu Ile Leu Pro His Pro Tyr Ile Ala Glu Arg Leu Phe 225 230 235 240 Val Leu Glu Ser Leu Gln Glu Ile Ala Pro His Phe Ile His Pro Ile 245 250 255 Leu Lys Gln Pro Ile Arg Asn Leu Tyr Asp Ala Leu Lys Lys 260 265 270 <210> 31 <211> 2250 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (2250) <223> Streptococcus pneumoniae spr0304 gene <400> 31 atgaagtgga caaaaagagt aatccgttat gcgaccaaaa atcggaaatc gccggctgaa 60 aacagacgca gagttggaaa aagtctgagt ttattatctg tctttgtttt tgccattttt 120 ttagtcaatt ttgcggtcat tattgggaca ggcactcgct ttggaacaga tttagcgaag 180 gaagctaaga aggttcatca aaccacccgt acagttcctg ccaaacgtgg gactatttat 240 gaccgaaatg gagtcccgat tgctgaggat gcaacctctt ataatgtcta tgcggtcatt 300 gatgagaact ataagtcagc aacgggtaag attctttacg tagaaaaaac acaatttaac 360 aaggttgcag aggtctttca taagtatctg gacatggaag aatcctatgt aagagagcaa 420 ctctcgcaac ctaatctcaa gcaagtttcc tttggagcaa agggaaatgg gattacctat 480 gccaatatga tgtctatcaa aaaagaattg gaagctgcag aggtcaaggg gattgatttt 540 acaaccagtc ccaatcgtag ttacccaaac ggacaatttg cttctagttt tatcggccta 600 gctcagctcc atgaaaatga agatggaagc aagagcttgc tgggaacctc tggaatggag 660 agttccttga acagtattct tgcagggaca gacggcatta ttacctatga aaaggatcgt 720 ctgggtaata ttgtacccgg aacagaacaa gtttcccaac gaacgatgga cggtaaggat 780 gtttatacaa ccatttccag ccccctccag tcctttatgg aaacccagat ggatgctttt 840 caagagaagg taaaaggaaa gtacatgaca gcgactttgg tcagtgctaa aacaggggaa 900 attctggcaa caacgcaacg accgaccttt gatgcagata caaaagaagg cattacagag 960 gactttgttt ggcgtgatat cctttaccaa agtaactatg agccaggttc cactatgaaa 1020 gtgatgatgt tggctgctgc tattgataat aatacctttc caggaggaga agtctttaat 1080 agtagtgagt taaaaattgc agatgccacg attcgagatt gggacgttaa tgaaggattg 1140 actggtggca gaatgatgac tttttctcaa ggttttgcac actcaagtaa cgttgggatg 1200 accctccttg agcaaaagat gggagatgct acctggcttg attatcttaa tcgttttaaa 1260 tttggtgttc cgacccgttt cggtttgacg gatgagtatg ctggtcagct tcctgcggat 1320 aatattgtca acattgcgca aagctcattt ggacaaggga tttcagtgac ccagacgcaa 1380 atgattcgtg cctttacagc tattgctaat gacggtgtca tgctggagcc taaatttatt 1440 agtgccattt atgatccaaa tgatcaaact gctcggaaat ctcaaaaaga aattgtggga 1500 aatcctgttt ctaaagatgc agctagtcta actcggacta acatggtttt ggtagggacg 1560 gatccggttt atggaaccat gtataaccac agcacaggca agccaactgt aactgttcct 1620 gggcaaaatg tagccctcaa gtctggtacg gctcagattg ctgacgagaa aaatggtggt 1680 tatctagtcg ggttaaccga ctatattttc tcggctgtat cgatgagtcc ggctgaaaat 1740 cctgatttta tcttgtatgt gacggtccaa caacctgaac attattcagg tattcagttg 1800 ggagaatttg ccaatcctat cttggagcgg gcttcagcta tgaaagactc tctcaatctt 1860 caaacaacag ctaaggcttt ggagcaagta agtcaacaaa gtccttatcc tatgcccagt 1920 gtcaaggata tttcacctgg tgatttagca gaagaattgc gtcgcaatct tgtacaaccc 1980 atcgttgtgg gaacaggaac gaagattaaa aacagttctg ctgaagaagg gaagaatctt 2040 gccccgaacc agcaagtcct tatcttatct gataaagcag aggaggttcc agatatgtat 2100 ggttggacaa aggagactgc tgagaccctt gctaagtggc tcaatataga acttgaattt 2160 caaggctcgg gctctactgt gcagaagcaa gatgttcgtg ctaacacagc tatcaaggac 2220 attaaaaaaa ttacattaac tttaggagac 2250 <210> 32 <211> 750 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (750) <223> Penicillin-binding protein 2X <400> 32 Met Lys Trp Thr Lys Arg Val Ile Arg Tyr Ala Thr Lys Asn Arg Lys 1 5 10 15 Ser Pro Ala Glu Asn Arg Arg Arg Val Gly Lys Ser Leu Ser Leu Leu 20 25 30 Ser Val Phe Val Phe Ala Ile Phe Leu Val Asn Phe Ala Val Ile Ile 35 40 45 Gly Thr Gly Thr Arg Phe Gly Thr Asp Leu Ala Lys Glu Ala Lys Lys 50 55 60 Val His Gln Thr Thr Arg Thr Val Pro Ala Lys Arg Gly Thr Ile Tyr 65 70 75 80 Asp Arg Asn Gly Val Pro Ile Ala Glu Asp Ala Thr Ser Tyr Asn Val 85 90 95 Tyr Ala Val Ile Asp Glu Asn Tyr Lys Ser Ala Thr Gly Lys Ile Leu 100 105 110 Tyr Val Glu Lys Thr Gln Phe Asn Lys Val Ala Glu Val Phe His Lys 115 120 125 Tyr Leu Asp Met Glu Glu Ser Tyr Val Arg Glu Gln Leu Ser Gln Pro 130 135 140 Asn Leu Lys Gln Val Ser Phe Gly Ala Lys Gly Asn Gly Ile Thr Tyr 145 150 155 160 Ala Asn Met Met Ser Ile Lys Lys Glu Leu Glu Ala Ala Glu Val Lys 165 170 175 Gly Ile Asp Phe Thr Thr Ser Pro Asn Arg Ser Tyr Pro Asn Gly Gln 180 185 190 Phe Ala Ser Ser Phe Ile Gly Leu Ala Gln Leu His Glu Asn Glu Asp 195 200 205 Gly Ser Lys Ser Leu Leu Gly Thr Ser Gly Met Glu Ser Ser Leu Asn 210 215 220 Ser Ile Leu Ala Gly Thr Asp Gly Ile Ile Thr Tyr Glu Lys Asp Arg 225 230 235 240 Leu Gly Asn Ile Val Pro Gly Thr Glu Gln Val Ser Gln Arg Thr Met 245 250 255 Asp Gly Lys Asp Val Tyr Thr Thr Ile Ser Ser Pro Leu Gln Ser Phe 260 265 270 Met Glu Thr Gln Met Asp Ala Phe Gln Glu Lys Val Lys Gly Lys Tyr 275 280 285 Met Thr Ala Thr Leu Val Ser Ala Lys Thr Gly Glu Ile Leu Ala Thr 290 295 300 Thr Gln Arg Pro Thr Phe Asp Ala Asp Thr Lys Glu Gly Ile Thr Glu 305 310 315 320 Asp Phe Val Trp Arg Asp Ile Leu Tyr Gln Ser Asn Tyr Glu Pro Gly 325 330 335 Ser Thr Met Lys Val Met Met Leu Ala Ala Ala Ile Asp Asn Asn Thr 340 345 350 Phe Pro Gly Gly Glu Val Phe Asn Ser Ser Glu Leu Lys Ile Ala Asp 355 360 365 Ala Thr Ile Arg Asp Trp Asp Val Asn Glu Gly Leu Thr Gly Gly Arg 370 375 380 Met Met Thr Phe Ser Gln Gly Phe Ala His Ser Ser Asn Val Gly Met 385 390 395 400 Thr Leu Leu Glu Gln Lys Met Gly Asp Ala Thr Trp Leu Asp Tyr Leu 405 410 415 Asn Arg Phe Lys Phe Gly Val Pro Thr Arg Phe Gly Leu Thr Asp Glu 420 425 430 Tyr Ala Gly Gln Leu Pro Ala Asp Asn Ile Val Asn Ile Ala Gln Ser 435 440 445 Ser Phe Gly Gln Gly Ile Ser Val Thr Gln Thr Gln Met Ile Arg Ala 450 455 460 Phe Thr Ala Ile Ala Asn Asp Gly Val Met Leu Glu Pro Lys Phe Ile 465 470 475 480 Ser Ala Ile Tyr Asp Pro Asn Asp Gln Thr Ala Arg Lys Ser Gln Lys 485 490 495 Glu Ile Val Gly Asn Pro Val Ser Lys Asp Ala Ala Ser Leu Thr Arg 500 505 510 Thr Asn Met Val Leu Val Gly Thr Asp Pro Val Tyr Gly Thr Met Tyr 515 520 525 Asn His Ser Thr Gly Lys Pro Thr Val Thr Val Pro Gly Gln Asn Val 530 535 540 Ala Leu Lys Ser Gly Thr Ala Gln Ile Ala Asp Glu Lys Asn Gly Gly 545 550 555 560 Tyr Leu Val Gly Leu Thr Asp Tyr Ile Phe Ser Ala Val Ser Met Ser 565 570 575 Pro Ala Glu Asn Pro Asp Phe Ile Leu Tyr Val Thr Val Gln Gln Pro 580 585 590 Glu His Tyr Ser Gly Ile Gln Leu Gly Glu Phe Ala Asn Pro Ile Leu 595 600 605 Glu Arg Ala Ser Ala Met Lys Asp Ser Leu Asn Leu Gln Thr Thr Ala 610 615 620 Lys Ala Leu Glu Gln Val Ser Gln Gln Ser Pro Tyr Pro Met Pro Ser 625 630 635 640 Val Lys Asp Ile Ser Pro Gly Asp Leu Ala Glu Glu Leu Arg Arg Asn 645 650 655 Leu Val Gln Pro Ile Val Val Gly Thr Gly Thr Lys Ile Lys Asn Ser 660 665 670 Ser Ala Glu Glu Gly Lys Asn Leu Ala Pro Asn Gln Gln Val Leu Ile 675 680 685 Leu Ser Asp Lys Ala Glu Glu Val Pro Asp Met Tyr Gly Trp Thr Lys 690 695 700 Glu Thr Ala Glu Thr Leu Ala Lys Trp Leu Asn Ile Glu Leu Glu Phe 705 710 715 720 Gln Gly Ser Gly Ser Thr Val Gln Lys Gln Asp Val Arg Ala Asn Thr 725 730 735 Ala Ile Lys Asp Ile Lys Lys Ile Thr Leu Thr Leu Gly Asp 740 745 750 <210> 33 <211> 876 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (876) <223> Streptococcus pneumoniae spr0338 gene <400> 33 atgacaaaaa aagttggtgt cggtcaggca catagtaaga taattttaat aggggaacat 60 gcggtcgttt acggttatcc tgccatttcc ctgcctcttt tggaagtgga ggtgacctgt 120 aaggtagttc ctgcagagag tccttggcgc ctttatgagg aggatacctt gtccatggcg 180 gtttatgcct cactggagta tttgaatatc acagaagcct gcattcgttg tgagattgac 240 tcggctatcc ctgagaaacg ggggatgggt tcgtcagcgg ctatcagcat agcggccatt 300 cgtgcggtat ttgactacta tcaggctgat ctgcctcatg atgtactaga aatcttggtc 360 aatcgagctg aaatgattgc ccatatgaat cctagtggtt tggatgctaa gacctgtctc 420 agtgaccaac ctattcgctt tatcaagaac gtaggattta cagaacttga gatggattta 480 tccgcctatt tggtgattgc cgatacgggt gtttatggtc atactcgtga agccatccaa 540 gtggttcaaa ataagggcaa ggatgcccta ccgtttttgc atgccttggg agaattaacc 600 cagcaggcag aaattgcgat ttcacaaaaa gatgctgaag ggctgggaca aatcctcagt 660 caagcacatt tacatttaaa agaaattggt gtcagtagcc ttgaggcaga ctctttggtt 720 gaaacagctc ttagtcatgg tgctctgggt gccaagatga gcggtggtgg gctaggaggt 780 tgtatcatag ccttggtaac caatttgaca cacgcacaag aactagcaga aagattagaa 840 gagaaaggag ctgttcagac atggatagag agcctg 876 <210> 34 <211> 292 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (292) <223> Mevalonate kinase <400> 34 Met Thr Lys Lys Val Gly Val Gly Gln Ala His Ser Lys Ile Ile Leu 1 5 10 15 Ile Gly Glu His Ala Val Val Tyr Gly Tyr Pro Ala Ile Ser Leu Pro 20 25 30 Leu Leu Glu Val Glu Val Thr Cys Lys Val Val Pro Ala Glu Ser Pro 35 40 45 Trp Arg Leu Tyr Glu Glu Asp Thr Leu Ser Met Ala Val Tyr Ala Ser 50 55 60 Leu Glu Tyr Leu Asn Ile Thr Glu Ala Cys Ile Arg Cys Glu Ile Asp 65 70 75 80 Ser Ala Ile Pro Glu Lys Arg Gly Met Gly Ser Ser Ala Ala Ile Ser 85 90 95 Ile Ala Ala Ile Arg Ala Val Phe Asp Tyr Tyr Gln Ala Asp Leu Pro 100 105 110 His Asp Val Leu Glu Ile Leu Val Asn Arg Ala Glu Met Ile Ala His 115 120 125 Met Asn Pro Ser Gly Leu Asp Ala Lys Thr Cys Leu Ser Asp Gln Pro 130 135 140 Ile Arg Phe Ile Lys Asn Val Gly Phe Thr Glu Leu Glu Met Asp Leu 145 150 155 160 Ser Ala Tyr Leu Val Ile Ala Asp Thr Gly Val Tyr Gly His Thr Arg 165 170 175 Glu Ala Ile Gln Val Val Gln Asn Lys Gly Lys Asp Ala Leu Pro Phe 180 185 190 Leu His Ala Leu Gly Glu Leu Thr Gln Gln Ala Glu Ile Ala Ile Ser 195 200 205 Gln Lys Asp Ala Glu Gly Leu Gly Gln Ile Leu Ser Gln Ala His Leu 210 215 220 His Leu Lys Glu Ile Gly Val Ser Ser Leu Glu Ala Asp Ser Leu Val 225 230 235 240 Glu Thr Ala Leu Ser His Gly Ala Leu Gly Ala Lys Met Ser Gly Gly 245 250 255 Gly Leu Gly Gly Cys Ile Ile Ala Leu Val Thr Asn Leu Thr His Ala 260 265 270 Gln Glu Leu Ala Glu Arg Leu Glu Glu Lys Gly Ala Val Gln Thr Trp 275 280 285 Ile Glu Ser Leu 290 <210> 35 <211> 1767 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1767) <223> Streptococcus pneumoniae spr0356 gene <400> 35 atggaagaaa aagtatcatt gaaagtcagg gttcaaaaac tagggacatc gctttcaaat 60 atggttatgc ccaatattgg agcatttatt gcttggggag tattgactgc cctctttatc 120 gctgatggct atctgccaaa tgaacagtta gctactgttg ttggtcctat gttaacgtat 180 ttattgccaa tcctgattgg ttacacaggt ggatatatga tccatggcca acgtggtgcc 240 gttgtaggat ctattgctac tgttggtgca atcacaggtt ctagtgttcc tatgtttatc 300 ggagctatgg taatgggccc actgggagga tggactatca agaaatttga tgagaagttc 360 caagaaaaaa ttcgtcccgg atttgaaatg ttagttaata acttctcagc tggtctcgtt 420 ggttttgcat tattgctttt ggctttctac gcaatcggtc cagtcgtatc gactcttact 480 ggagctgttg ggaatggtgt tgaggctatt gtcaatgctc gcctccttcc tatggctaat 540 attatcatcg aaccggctaa agtccttttc ctcaataatg ccctcaatca tggcattttt 600 actcctctgg gagtagaaca ggtagctcaa gctggtaagt caattctctt cctattggaa 660 gctaatcctg gaccaggcct gggaattcta ttagcttatg ctgtattcgg taaaggttct 720 gctaaatctt cttcttgggg ggcaatggtt attcatttct tcggagggat tcatgaaatt 780 tactttcctt atgttatgat gaagcctact ctatttttag ctgctatggc aggaggtatc 840 tctggaactt ttacttttca actcttagac gctggtctta aatctccagc ttcaccaggt 900 tctattattg cgattatggc tacggcgcca aaaggtgttt ggccccatct aaatattctt 960 ttaggtgttt tagtggcagc agttgtttct ttccttatag cagcccttat tcttcatgca 1020 gacaagtcaa ctgaggattc gctcgaagct gctcaggcgg ctacccaagc agctaaggct 1080 cagtctaaag gtcagttagt atcaacttct gttgatgcag ttgtttcgac agactcagtg 1140 gaaaaaatca ttttcgcctg cgatgctggt atgggaagct ctgctatggg agctagtatt 1200 cttcgagata aggttaaaaa agcaggtcta gagcttccag tatctaatca ggcaatctca 1260 aatttgcttg atacaccaaa aacattaatt gttactcagg aagaactgac accaagagct 1320 aaagacaaga gtccaagtgc tattcatgtt tctgttgata atttcttagc gtcccctcgt 1380 tatgatgaaa ttgtagcttc attaacagga gcttctccaa tagcagaaat tgaaggagat 1440 ataccaactt cagcaccagt agatagtcag gaaattgacc ttaaccatat tgatgctgta 1500 gtagttgctt atggtaaagc acagggaact gcaactatgg gctgtgaaac gattcgggct 1560 atctttagaa acaagaatat tcgtattcca gtttctactg ccaaaatttc agaattaggt 1620 gaatttaatt ctaaaaacat aatgattgta acaactattt ctttacaggc agaagtgcag 1680 caagcagcac cgaattctca atttcttatt gtggatagtt tagtaacaac accagaatat 1740 gacaaaatgg ctgctagaat gtacaaa 1767 <210> 36 <211> 589 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (589) <223> Mannitol PTS EII <400> 36 Met Glu Glu Lys Val Ser Leu Lys Val Arg Val Gln Lys Leu Gly Thr 1 5 10 15 Ser Leu Ser Asn Met Val Met Pro Asn Ile Gly Ala Phe Ile Ala Trp 20 25 30 Gly Val Leu Thr Ala Leu Phe Ile Ala Asp Gly Tyr Leu Pro Asn Glu 35 40 45 Gln Leu Ala Thr Val Val Gly Pro Met Leu Thr Tyr Leu Leu Pro Ile 50 55 60 Leu Ile Gly Tyr Thr Gly Gly Tyr Met Ile His Gly Gln Arg Gly Ala 65 70 75 80 Val Val Gly Ser Ile Ala Thr Val Gly Ala Ile Thr Gly Ser Ser Val 85 90 95 Pro Met Phe Ile Gly Ala Met Val Met Gly Pro Leu Gly Gly Trp Thr 100 105 110 Ile Lys Lys Phe Asp Glu Lys Phe Gln Glu Lys Ile Arg Pro Gly Phe 115 120 125 Glu Met Leu Val Asn Asn Phe Ser Ala Gly Leu Val Gly Phe Ala Leu 130 135 140 Leu Leu Leu Ala Phe Tyr Ala Ile Gly Pro Val Val Ser Thr Leu Thr 145 150 155 160 Gly Ala Val Gly Asn Gly Val Glu Ala Ile Val Asn Ala Arg Leu Leu 165 170 175 Pro Met Ala Asn Ile Ile Ile Glu Pro Ala Lys Val Leu Phe Leu Asn 180 185 190 Asn Ala Leu Asn His Gly Ile Phe Thr Pro Leu Gly Val Glu Gln Val 195 200 205 Ala Gln Ala Gly Lys Ser Ile Leu Phe Leu Leu Glu Ala Asn Pro Gly 210 215 220 Pro Gly Leu Gly Ile Leu Leu Ala Tyr Ala Val Phe Gly Lys Gly Ser 225 230 235 240 Ala Lys Ser Ser Ser Trp Gly Ala Met Val Ile His Phe Phe Gly Gly 245 250 255 Ile His Glu Ile Tyr Phe Pro Tyr Val Met Met Lys Pro Thr Leu Phe 260 265 270 Leu Ala Ala Met Ala Gly Gly Ile Ser Gly Thr Phe Thr Phe Gln Leu 275 280 285 Leu Asp Ala Gly Leu Lys Ser Pro Ala Ser Pro Gly Ser Ile Ile Ala 290 295 300 Ile Met Ala Thr Ala Pro Lys Gly Val Trp Pro His Leu Asn Ile Leu 305 310 315 320 Leu Gly Val Leu Val Ala Ala Val Val Ser Phe Leu Ile Ala Ala Leu 325 330 335 Ile Leu His Ala Asp Lys Ser Thr Glu Asp Ser Leu Glu Ala Ala Gln 340 345 350 Ala Ala Thr Gln Ala Ala Lys Ala Gln Ser Lys Gly Gln Leu Val Ser 355 360 365 Thr Ser Val Asp Ala Val Val Ser Thr Asp Ser Val Glu Lys Ile Ile 370 375 380 Phe Ala Cys Asp Ala Gly Met Gly Ser Ser Ala Met Gly Ala Ser Ile 385 390 395 400 Leu Arg Asp Lys Val Lys Lys Ala Gly Leu Glu Leu Pro Val Ser Asn 405 410 415 Gln Ala Ile Ser Asn Leu Leu Asp Thr Pro Lys Thr Leu Ile Val Thr 420 425 430 Gln Glu Glu Leu Thr Pro Arg Ala Lys Asp Lys Ser Pro Ser Ala Ile 435 440 445 His Val Ser Val Asp Asn Phe Leu Ala Ser Pro Arg Tyr Asp Glu Ile 450 455 460 Val Ala Ser Leu Thr Gly Ala Ser Pro Ile Ala Glu Ile Glu Gly Asp 465 470 475 480 Ile Pro Thr Ser Ala Pro Val Asp Ser Gln Glu Ile Asp Leu Asn His 485 490 495 Ile Asp Ala Val Val Val Ala Tyr Gly Lys Ala Gln Gly Thr Ala Thr 500 505 510 Met Gly Cys Glu Thr Ile Arg Ala Ile Phe Arg Asn Lys Asn Ile Arg 515 520 525 Ile Pro Val Ser Thr Ala Lys Ile Ser Glu Leu Gly Glu Phe Asn Ser 530 535 540 Lys Asn Ile Met Ile Val Thr Thr Ile Ser Leu Gln Ala Glu Val Gln 545 550 555 560 Gln Ala Ala Pro Asn Ser Gln Phe Leu Ile Val Asp Ser Leu Val Thr 565 570 575 Thr Pro Glu Tyr Asp Lys Met Ala Ala Arg Met Tyr Lys 580 585 <210> 37 <211> 1134 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1134) <223> Streptococcus pneumoniae spr0359 gene <400> 37 atgaaacatt ctgttcattt tggtgccggt aatatcggtc gtggttttat aggtgaaatt 60 ctatttaaaa atggtttcca tattgatttt gtggatgtca ataatcagat aattcatgct 120 ctaaatgaaa agggcaagta tgaaattgaa attgcacaga aaggacagtc tcgtatagaa 180 gtaactaatg tggctggcat taatagcaaa gaacatcctg agcaagtcat tgaagcgatt 240 caaaagacgg atattattac tactgcaatc ggacctaata tactcccttt tatcgctgaa 300 cttctagcca aaggaatcga agctcgccga gttgcaggga atacacaggt attggatgtt 360 atggcctgtg aaaatatgat tggcgggtct caatttcttt atcaagaagt caagaaatat 420 ttaagtccgg aaggtttgac atttgctgat aactacatag gttttccaaa tgctgcagta 480 gacaggattg ttccagcaca aagtcacgaa gattcccttt ttgttgtggt cgagcccttt 540 aatgaatggg tcgtggaaac caagcgtctt aaaaatccag atttacgtct aaaagatgtg 600 cattatgaag aagatttaga accctttatt gagcgaaaac ttttttcagt caattctgga 660 catgcaactt cagcttacat tggtgcgcat tatggtgcca agacaatttt ggaagctctt 720 caaaatccta atatcaaatc tcggattgaa tctgtattag ctgaaattcg gagtctcttg 780 attgccaaat ggaactttga taaaaaagaa ttggagaatt atcacaaagt cattatagaa 840 cggtttgaaa accctttcat agtggacgaa gttagtcgcg tagctcgtac tccaatccga 900 aaattaggct ataatgaacg attcatccgg ccgatacgtg aattgaaaga actcagtttg 960 tcatataaaa acctacttaa aacagttggc tatgcctttg actatcgcga tgtaaatgat 1020 gaagaaagta ttcgattagg tgaattgttg gctaaacaat tagtcaaaga tgttgttata 1080 caagttacag gtttagacga ccaagaattg attgagcaaa ttgtagagta tatt 1134 <210> 38 <211> 378 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (378) Mannitol-1-phosphate 5-dehydrogenase <400> 38 Met Lys His Ser Val His Phe Gly Ala Gly Asn Ile Gly Arg Gly Phe 1 5 10 15 Ile Gly Glu Ile Leu Phe Lys Asn Gly Phe His Ile Asp Phe Val Asp 20 25 30 Val Asn Asn Gln Ile Ile His Ala Leu Asn Glu Lys Gly Lys Tyr Glu 35 40 45 Ile Glu Ile Ala Gln Lys Gly Gln Ser Arg Ile Glu Val Thr Asn Val 50 55 60 Ala Gly Ile Asn Ser Lys Glu His Pro Glu Gln Val Ile Glu Ala Ile 65 70 75 80 Gln Lys Thr Asp Ile Ile Thr Thr Ala Ile Gly Pro Asn Ile Leu Pro 85 90 95 Phe Ile Ala Glu Leu Leu Ala Lys Gly Ile Glu Ala Arg Arg Val Ala 100 105 110 Gly Asn Thr Gln Val Leu Asp Val Met Ala Cys Glu Asn Met Ile Gly 115 120 125 Gly Ser Gln Phe Leu Tyr Gln Glu Val Lys Lys Tyr Leu Ser Pro Glu 130 135 140 Gly Leu Thr Phe Ala Asp Asn Tyr Ile Gly Phe Pro Asn Ala Ala Val 145 150 155 160 Asp Arg Ile Val Pro Ala Gln Ser His Glu Asp Ser Leu Phe Val Val 165 170 175 Val Glu Pro Phe Asn Glu Trp Val Val Glu Thr Lys Arg Leu Lys Asn 180 185 190 Pro Asp Leu Arg Leu Lys Asp Val His Tyr Glu Glu Asp Leu Glu Pro 195 200 205 Phe Ile Glu Arg Lys Leu Phe Ser Val Asn Ser Gly His Ala Thr Ser 210 215 220 Ala Tyr Ile Gly Ala His Tyr Gly Ala Lys Thr Ile Leu Glu Ala Leu 225 230 235 240 Gln Asn Pro Asn Ile Lys Ser Arg Ile Glu Ser Val Leu Ala Glu Ile 245 250 255 Arg Ser Leu Leu Ile Ala Lys Trp Asn Phe Asp Lys Lys Glu Leu Glu 260 265 270 Asn Tyr His Lys Val Ile Ile Glu Arg Phe Glu Asn Pro Phe Ile Val 275 280 285 Asp Glu Val Ser Arg Val Ala Arg Thr Pro Ile Arg Lys Leu Gly Tyr 290 295 300 Asn Glu Arg Phe Ile Arg Pro Ile Arg Glu Leu Lys Glu Leu Ser Leu 305 310 315 320 Ser Tyr Lys Asn Leu Leu Lys Thr Val Gly Tyr Ala Phe Asp Tyr Arg 325 330 335 Asp Val Asn Asp Glu Glu Ser Ile Arg Leu Gly Glu Leu Leu Ala Lys 340 345 350 Gln Leu Val Lys Asp Val Val Ile Gln Val Thr Gly Leu Asp Asp Gln 355 360 365 Glu Leu Ile Glu Gln Ile Val Glu Tyr Ile 370 375 <210> 39 <211> 1335 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1335) <223> Streptococcus pneumoniae spr0372 gene <400> 39 atggaaccgt ggccctgcca ctccaacgtt ttgtcagggt gcttttttca taaaggagtt 60 cttatgttag atatcaaacg tattcgtaca gattttgaag ctgtcgcaga aaaattagct 120 acacgtggtg tagatgctgc tgtcttgaat gaaatgaaag aaatcgatgc taaacgtcgt 180 aacatcttgg tcaaggttga aactctcaaa gcagaacgta acacagtttc tgctgagatt 240 gcccaagcta agcgcaacaa ggaaaataca gatgacaaga ttgctgccat gcaaaatcta 300 tctgctgagg ttaaagcctt ggatgctgaa ttggcagaaa tcgatgctaa attgacagaa 360 tttacaacga ctcttccaaa tatcccagct gacagcgttc ctgttggggc tgacgaagac 420 gacaatgtgg aagttcgccg ttggggtact ccacgcgagt ttgacttcga acctaaagct 480 cactgggatc tcggtgaaga ccttggtatc cttgactggg aacgcggtgg taaggtaaca 540 ggcgctcgct tcctcttcta taaaggcctc ggtgctcgtt tggaacgtgc tatctacaac 600 tttatgttgg atgaacatgg aaaagaaggc tatactgaag tcatcacacc ttacatagtc 660 aaccatgatt ctatgtttgg tactggtcag tatccaaaat ttaaggaaga tacttttgaa 720 ctcagcgata ccaactttgt cttgattcca actgctgaag ttcctctgac aaactactac 780 cgtgatgaaa tcttagacgg caaagatctt ccaatctact tcactgctat gagtccgtca 840 ttccgttctg aggctggttc tgccggtcgt gatacgcgtg gcttgatccg tttgcaccaa 900 ttccacaagg ttgaaatggt caaatttgcc aaaccagaag aatcttacga agaattggaa 960 aaaatgacag ccaacgctga aaacattctt caaaaactca accttccata ccgtgtcgtt 1020 gctctctcta ctggagatat gggcttctca gctacgaaga cttacgactt ggaagtgtgg 1080 attccagcac aaaacaatta ccgtgaaatc tcaagctgtt caaacacaga agatttccaa 1140 gcccgtcgtg cccaaatccg ttaccgtgat gaagcagatg gcaaggtgaa actccttcat 1200 accttgaacg gttctggact tgcagttgga cgtacagtgg ctgcaattct tgaaaattac 1260 caaaatgaag atggttctgt gaccatccca gaagcacttc gtccatacat gggtggagct 1320 gaagtcatca aacca 1335 <210> 40 <211> 445 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (445) <223> Seryl-tRNA synthetase <400> 40 Met Glu Pro Trp Pro Cys His Ser Asn Val Leu Ser Gly Cys Phe Phe 1 5 10 15 His Lys Gly Val Leu Met Leu Asp Ile Lys Arg Ile Arg Thr Asp Phe 20 25 30 Glu Ala Val Ala Glu Lys Leu Ala Thr Arg Gly Val Asp Ala Ala Val 35 40 45 Leu Asn Glu Met Lys Glu Ile Asp Ala Lys Arg Arg Asn Ile Leu Val 50 55 60 Lys Val Glu Thr Leu Lys Ala Glu Arg Asn Thr Val Ser Ala Glu Ile 65 70 75 80 Ala Gln Ala Lys Arg Asn Lys Glu Asn Thr Asp Asp Lys Ile Ala Ala 85 90 95 Met Gln Asn Leu Ser Ala Glu Val Lys Ala Leu Asp Ala Glu Leu Ala 100 105 110 Glu Ile Asp Ala Lys Leu Thr Glu Phe Thr Thr Thr Leu Pro Asn Ile 115 120 125 Pro Ala Asp Ser Val Pro Val Gly Ala Asp Glu Asp Asp Asn Val Glu 130 135 140 Val Arg Arg Trp Gly Thr Pro Arg Glu Phe Asp Phe Glu Pro Lys Ala 145 150 155 160 His Trp Asp Leu Gly Glu Asp Leu Gly Ile Leu Asp Trp Glu Arg Gly 165 170 175 Gly Lys Val Thr Gly Ala Arg Phe Leu Phe Tyr Lys Gly Leu Gly Ala 180 185 190 Arg Leu Glu Arg Ala Ile Tyr Asn Phe Met Leu Asp Glu His Gly Lys 195 200 205 Glu Gly Tyr Thr Glu Val Ile Thr Pro Tyr Ile Val Asn His Asp Ser 210 215 220 Met Phe Gly Thr Gly Gln Tyr Pro Lys Phe Lys Glu Asp Thr Phe Glu 225 230 235 240 Leu Ser Asp Thr Asn Phe Val Leu Ile Pro Thr Ala Glu Val Pro Leu 245 250 255 Thr Asn Tyr Tyr Arg Asp Glu Ile Leu Asp Gly Lys Asp Leu Pro Ile 260 265 270 Tyr Phe Thr Ala Met Ser Pro Ser Phe Arg Ser Glu Ala Gly Ser Ala 275 280 285 Gly Arg Asp Thr Arg Gly Leu Ile Arg Leu His Gln Phe His Lys Val 290 295 300 Glu Met Val Lys Phe Ala Lys Pro Glu Glu Ser Tyr Glu Glu Leu Glu 305 310 315 320 Lys Met Thr Ala Asn Ala Glu Asn Ile Leu Gln Lys Leu Asn Leu Pro 325 330 335 Tyr Arg Val Val Ala Leu Ser Thr Gly Asp Met Gly Phe Ser Ala Thr 340 345 350 Lys Thr Tyr Asp Leu Glu Val Trp Ile Pro Ala Gln Asn Asn Tyr Arg 355 360 365 Glu Ile Ser Ser Cys Ser Asn Thr Glu Asp Phe Gln Ala Arg Arg Ala 370 375 380 Gln Ile Arg Tyr Arg Asp Glu Ala Asp Gly Lys Val Lys Leu Leu His 385 390 395 400 Thr Leu Asn Gly Ser Gly Leu Ala Val Gly Arg Thr Val Ala Ala Ile 405 410 415 Leu Glu Asn Tyr Gln Asn Glu Asp Gly Ser Val Thr Ile Pro Glu Ala 420 425 430 Leu Arg Pro Tyr Met Gly Gly Ala Glu Val Ile Lys Pro 435 440 445 <210> 41 <211> 1440 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1440) <223> Streptococcus pneumoniae spr0393 gene <400> 41 atgaactttg aaacagtcat tggacttgaa gtccacgtag agctcaacac caattcaaaa 60 atcttctcac ctacttctgc ccacttcgga aatgatcaaa atgccaacac taacgtgatt 120 gactggtctt tcccaggagt tctgccagtt ctcaataaag gggttgtcga tgccggaatc 180 aaggctgctc ttgccctcaa catggacatc cacaaaaaga tgcactttga ccgcaagaac 240 tacttctatc ctgataaccc caaagcctac caaatttctc aatttgatga accaatcgga 300 tataatggct ggattgaagt cgaactagaa gacggtacga ccaagaaaat cggtatcgaa 360 cgtgcccacc tagaggaaga cgctggtaaa aacacccatg gtacagatgg ctactcttat 420 gttgacctca accgccaagg ggttcccttg attgagattg tatctgaggc agatatgcgt 480 tctcctgaag aagcctatgc ttatctgaca gccctcaagg aagttatcca gtacgctggc 540 atttctgacg ttaagatgga ggaaggttcg atgcgtgtgg atgccaacat ctcccttcgt 600 ccttatggtc aagagaaatt cggtaccaag actgaattga agaacctcaa ctccttctca 660 aacgttcgta aaggtcttga atacgaagtc caacgccagg ctgaaattct tcgctcaggt 720 ggtcaaatcc gccaagaaac acgccgttac gatgaagcga ataaagcaac catcctcatg 780 cgtgtcaagg aaggggctgc tgactaccgc tacttcccag aaccagacct acccctcttt 840 gaaatttctg acgagtggat tgaggaaatg cggactgagt tgccagagtt tccaaaagaa 900 cgtcgtgcgc gttatgtatc tgaccttggt ttatcagact acgatgctag tcagttgact 960 gctaataaag tcacttctga cttctttgaa aaagctgttg ccctaggtgg tgatgccaaa 1020 caagtctcta actggctcca aggggaagtc gctcagttct tgaatgctga aggtaaaaca 1080 ctggaacaaa tcgaattgac accagaaaac ttggttgaaa tgattgccat catcgaagac 1140 ggtactattt catctaagat tgccaagaaa gtctttgtcc atctagctaa aaatggcggt 1200 ggcgcgcgtg aatacgtgga aaaagcaggt atggttcaaa tttcagatcc agctatcttg 1260 atcccaatca tccaccaagt ctttgccgat aacgaagctg ctgttgccga cttcaagtca 1320 ggcaaacgta acgccgacaa agcctttaca ggattcctta tgaaggcaac caaaggccaa 1380 gccaacccac aagttgccct taaactactt gcacaggaat tggcgaagtt gaaagaaaac 1440 1440 <210> 42 <211> 480 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (480) Glutamyl tRNA-Gln amidotransferase subunit B <400> 42 Met Asn Phe Glu Thr Val Ile Gly Leu Glu Val His Val Glu Leu Asn 1 5 10 15 Thr Asn Ser Lys Ile Phe Ser Pro Thr Ser Ala His Phe Gly Asn Asp 20 25 30 Gln Asn Ala Asn Thr Asn Val Ile Asp Trp Ser Phe Pro Gly Val Leu 35 40 45 Pro Val Leu Asn Lys Gly Val Val Asp Ala Gly Ile Lys Ala Ala Leu 50 55 60 Ala Leu Asn Met Asp Ile His Lys Lys Met His Phe Asp Arg Lys Asn 65 70 75 80 Tyr Phe Tyr Pro Asp Asn Pro Lys Ala Tyr Gln Ile Ser Gln Phe Asp 85 90 95 Glu Pro Ile Gly Tyr Asn Gly Trp Ile Glu Val Glu Leu Glu Asp Gly 100 105 110 Thr Thr Lys Lys Ile Gly Ile Glu Arg Ala His Leu Glu Glu Asp Ala 115 120 125 Gly Lys Asn Thr His Gly Thr Asp Gly Tyr Ser Tyr Val Asp Leu Asn 130 135 140 Arg Gln Gly Val Pro Leu Ile Glu Ile Val Ser Glu Ala Asp Met Arg 145 150 155 160 Ser Pro Glu Glu Ala Tyr Ala Tyr Leu Thr Ala Leu Lys Glu Val Ile 165 170 175 Gln Tyr Ala Gly Ile Ser Asp Val Lys Met Glu Glu Gly Ser Met Arg 180 185 190 Val Asp Ala Asn Ile Ser Leu Arg Pro Tyr Gly Gln Glu Lys Phe Gly 195 200 205 Thr Lys Thr Glu Leu Lys Asn Leu Asn Ser Phe Ser Asn Val Arg Lys 210 215 220 Gly Leu Glu Tyr Glu Val Gln Arg Gln Ala Glu Ile Leu Arg Ser Gly 225 230 235 240 Gly Gln Ile Arg Gln Glu Thr Arg Arg Tyr Asp Glu Ala Asn Lys Ala 245 250 255 Thr Ile Leu Met Arg Val Lys Glu Gly Ala Ala Asp Tyr Arg Tyr Phe 260 265 270 Pro Glu Pro Asp Leu Pro Leu Phe Glu Ile Ser Asp Glu Trp Ile Glu 275 280 285 Glu Met Arg Thr Glu Leu Pro Glu Phe Pro Lys Glu Arg Arg Ala Arg 290 295 300 Tyr Val Ser Asp Leu Gly Leu Ser Asp Tyr Asp Ala Ser Gln Leu Thr 305 310 315 320 Ala Asn Lys Val Thr Ser Asp Phe Phe Glu Lys Ala Val Ala Leu Gly 325 330 335 Gly Asp Ala Lys Gln Val Ser Asn Trp Leu Gln Gly Glu Val Ala Gln 340 345 350 Phe Leu Asn Ala Glu Gly Lys Thr Leu Glu Gln Ile Glu Leu Thr Pro 355 360 365 Glu Asn Leu Val Glu Met Ile Ala Ile Ile Glu Asp Gly Thr Ile Ser 370 375 380 Ser Lys Ile Ala Lys Lys Val Phe Val His Leu Ala Lys Asn Gly Gly 385 390 395 400 Gly Ala Arg Glu Tyr Val Glu Lys Ala Gly Met Val Gln Ile Ser Asp 405 410 415 Pro Ala Ile Leu Ile Pro Ile Ile His Gln Val Phe Ala Asp Asn Glu 420 425 430 Ala Ala Val Ala Asp Phe Lys Ser Gly Lys Arg Asn Ala Asp Lys Ala 435 440 445 Phe Thr Gly Phe Leu Met Lys Ala Thr Lys Gly Gln Ala Asn Pro Gln 450 455 460 Val Ala Leu Lys Leu Leu Ala Gln Glu Leu Ala Lys Leu Lys Glu Asn 465 470 475 480 <210> 43 <211> 300 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (300) <223> Streptococcus pneumoniae spr0395 gene <400> 43 atgaaaatta cgcaagaaga ggtaacacac gttgccaatc tttcaaaatt aagattctct 60 gaagaagaaa ctgctgcctt tgcgaccacc ttgtctaaga ttgttgacat ggttgaattg 120 ctgggcgaag ttgacacaac tggtgtcgca cctactacga ctatggctga ccgcaagact 180 gtactccgcc ctgatgtggc cgaagaagga acagaccgtg atcgcttgtt taaaaacgta 240 cctgaacaag acaactacta tatcaaggtg ccagctatcc tagacgatgg aggagatgcc 300 300 <210> 44 <211> 100 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (100) <223> Glutamyl tRNA-Gln amidotransferase, subunit C <400> 44 Met Lys Ile Thr Gln Glu Glu Val Thr His Val Ala Asn Leu Ser Lys 1 5 10 15 Leu Arg Phe Ser Glu Glu Glu Thr Ala Ala Phe Ala Thr Thr Leu Ser 20 25 30 Lys Ile Val Asp Met Val Glu Leu Leu Gly Glu Val Asp Thr Thr Gly 35 40 45 Val Ala Pro Thr Thr Thr Met Ala Asp Arg Lys Thr Val Leu Arg Pro 50 55 60 Asp Val Ala Glu Glu Gly Thr Asp Arg Asp Arg Leu Phe Lys Asn Val 65 70 75 80 Pro Glu Gln Asp Asn Tyr Tyr Ile Lys Val Pro Ala Ile Leu Asp Asp 85 90 95 Gly Gly Asp Ala 100 <210> 45 <211> 1134 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1134) <223> Streptococcus pneumoniae spr0478 gene <400> 45 atgagtaaag aaatgctaga ggccttccgc attttggaag aagacaagag aatcaaaaaa 60 gaagatatca tcgacgcagt agtagagtcg cttcgttccg cttatcgcag acgctatggt 120 cagtcagaca gcgtagctat tgacttcaac gaaaaaacag gtgactttac agtttatact 180 gtccgtgaag ttgttgatga agcatttgat agccgtttgg aaatcagctt gaaagatgct 240 cttgccatta attcagctta tgaacttgga gacaaaatca agtttgaaga agcaccagct 300 gagtttggtc gtgtagcagc ccaatctgcc aaacaaacca tcatggaaaa aatgcgcaag 360 caaacacgtg ccatcactta caatacttac aaagaacatg agcaagaaat catgtctggt 420 acagtagaac gctttgacaa ccgctttatc tatgtcaacc ttggtagcat cgaagcccaa 480 ttgtcaaaac aagaccaaat tcctggagaa gtttttgctt ctcatgatcg tatcgaagtt 540 tatgtttaca aggttgaaga caaccctcgt ggtgtgaacg tctttgttag ccgtagccat 600 ccagaaatga tcaaacgttt gatggagcaa gaaattccag aagtttatga tggaactgtt 660 gaaatcatga gcgtggctcg tgaagcaggt gaccgtacga aggttgctgt tcgtagccac 720 aatccaaacg tggatgctat cggtacaatc gttggacgtg gtggtgctaa tatcaagaag 780 attactagca aattccaccc agctcgttac gatgctaaaa atgaccgcat ggtaccaatc 840 gaagaaaata tcgatgttat cgagtgggta gcagatccag ctgaatttat ctacaatgcc 900 atcgctcctg ctgaggttga ccaagttatc tttgatgaaa gcgacagcaa acgtgccttg 960 gtggttgttc cagataacaa gctttctctt gccattggtc gtcgtggaca aaacgtgcgc 1020 ttggcggctc acttgactgg ttaccgtatc gatatcaagt ctgctagcga atttgaagcc 1080 atggaagacg ctgcttcagt agagttggaa gtagaaaacg atactgtaga agaa 1134 <210> 46 <211> 378 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (378) <223> Transcription elongation factor <400> 46 Met Ser Lys Glu Met Leu Glu Ala Phe Arg Ile Leu Glu Glu Asp Lys 1 5 10 15 Arg Ile Lys Lys Glu Asp Ile Ile Asp Ala Val Val Glu Ser Leu Arg 20 25 30 Ser Ala Tyr Arg Arg Arg Tyr Gly Gln Ser Asp Ser Val Ala Ile Asp 35 40 45 Phe Asn Glu Lys Thr Gly Asp Phe Thr Val Tyr Thr Val Arg Glu Val 50 55 60 Val Asp Glu Ala Phe Asp Ser Arg Leu Glu Ile Ser Leu Lys Asp Ala 65 70 75 80 Leu Ala Ile Asn Ser Ala Tyr Glu Leu Gly Asp Lys Ile Lys Phe Glu 85 90 95 Glu Ala Pro Ala Glu Phe Gly Arg Val Ala Ala Gln Ser Ala Lys Gln 100 105 110 Thr Ile Met Glu Lys Met Arg Lys Gln Thr Arg Ala Ile Thr Tyr Asn 115 120 125 Thr Tyr Lys Glu His Glu Gln Glu Ile Met Ser Gly Thr Val Glu Arg 130 135 140 Phe Asp Asn Arg Phe Ile Tyr Val Asn Leu Gly Ser Ile Glu Ala Gln 145 150 155 160 Leu Ser Lys Gln Asp Gln Ile Pro Gly Glu Val Phe Ala Ser His Asp 165 170 175 Arg Ile Glu Val Tyr Val Tyr Lys Val Glu Asp Asn Pro Arg Gly Val 180 185 190 Asn Val Phe Val Ser Arg Ser His Pro Glu Met Ile Lys Arg Leu Met 195 200 205 Glu Gln Glu Ile Pro Glu Val Tyr Asp Gly Thr Val Glu Ile Met Ser 210 215 220 Val Ala Arg Glu Ala Gly Asp Arg Thr Lys Val Ala Val Arg Ser His 225 230 235 240 Asn Pro Asn Val Asp Ala Ile Gly Thr Ile Val Gly Arg Gly Gly Ala 245 250 255 Asn Ile Lys Lys Ile Thr Ser Lys Phe His Pro Ala Arg Tyr Asp Ala 260 265 270 Lys Asn Asp Arg Met Val Pro Ile Glu Glu Asn Ile Asp Val Ile Glu 275 280 285 Trp Val Ala Asp Pro Ala Glu Phe Ile Tyr Asn Ala Ile Ala Pro Ala 290 295 300 Glu Val Asp Gln Val Ile Phe Asp Glu Ser Asp Ser Lys Arg Ala Leu 305 310 315 320 Val Val Val Pro Asp Asn Lys Leu Ser Leu Ala Ile Gly Arg Arg Gly 325 330 335 Gln Asn Val Arg Leu Ala Ala His Leu Thr Gly Tyr Arg Ile Asp Ile 340 345 350 Lys Ser Ala Ser Glu Phe Glu Ala Met Glu Asp Ala Ala Ser Val Glu 355 360 365 Leu Glu Val Glu Asn Asp Thr Val Glu Glu 370 375 <210> 47 <211> 879 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (879) <223> Streptococcus pneumoniae spr0530 gene <400> 47 atggcaatcg tttcagcaga aaaatttgtc caagcagccc gtgacaacgg ttatgcagtt 60 ggtggattta acacaaacaa ccttgagtgg actcaagcta tcttgcgcgc agcagaagct 120 aaaaaagctc cagttttgat ccaaacttca atgggtgctg ctaaatacat gggtggttac 180 aaagttgctc gcaacttgat cgctaacctt gttgaatcaa tgggtatcac tgtaccagta 240 gctatccacc ttgaccacgg tcactacgaa gatgcacttg agtgtatcga agttggttat 300 acttcaatca tgtttgacgg ttcacacctt ccagttgaag aaaaccttaa attggctaaa 360 gaagttgttg aaaaagcaca cgctaaaggt atctcagtag aagctgaagt tggtactatc 420 ggtggtgaag aagacggaat catcggtaaa ggtgaattgg ctccaatcga agacgctaaa 480 gcaatggttg aaactggtat cgacttcttg gcagctggta tcggtaacat ccacggccct 540 tacccagtaa actgggaagg tcttgacctt gaccacttgc aaaaattgac agaagctctt 600 ccaggattcc caatcgtatt gcacggtgga tcaggtattc ctgatgagca aatccaagca 660 gctatcaaac ttggtgttgc caaagttaac gttaacacag aatgccaaat cgcattcgct 720 aacgcaactc gtaaatttgc tcgtgactac gaagcaaacg aagcagaata cgacaagaaa 780 aaactcttcg acccacgtaa attcttggct gacggtgtaa aagctatcca agcatcggtt 840 gaagaacgta tcgacgtatt cggttcagaa ggtaaagca 879 <210> 48 <211> 293 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (293) <223> Fructose-bisphosphate aldolase <400> 48 Met Ala Ile Val Ser Ala Glu Lys Phe Val Gln Ala Ala Arg Asp Asn 1 5 10 15 Gly Tyr Ala Val Gly Gly Phe Asn Thr Asn Asn Leu Glu Trp Thr Gln 20 25 30 Ala Ile Leu Arg Ala Ala Glu Ala Lys Lys Ala Pro Val Leu Ile Gln 35 40 45 Thr Ser Met Gly Ala Ala Lys Tyr Met Gly Gly Tyr Lys Val Ala Arg 50 55 60 Asn Leu Ile Ala Asn Leu Val Glu Ser Met Gly Ile Thr Val Pro Val 65 70 75 80 Ala Ile His Leu Asp His Gly His Tyr Glu Asp Ala Leu Glu Cys Ile 85 90 95 Glu Val Gly Tyr Thr Ser Ile Met Phe Asp Gly Ser His Leu Pro Val 100 105 110 Glu Glu Asn Leu Lys Leu Ala Lys Glu Val Val Glu Lys Ala His Ala 115 120 125 Lys Gly Ile Ser Val Glu Ala Glu Val Gly Thr Ile Gly Gly Glu Glu 130 135 140 Asp Gly Ile Ile Gly Lys Gly Glu Leu Ala Pro Ile Glu Asp Ala Lys 145 150 155 160 Ala Met Val Glu Thr Gly Ile Asp Phe Leu Ala Ala Gly Ile Gly Asn 165 170 175 Ile His Gly Pro Tyr Pro Val Asn Trp Glu Gly Leu Asp Leu Asp His 180 185 190 Leu Gln Lys Leu Thr Glu Ala Leu Pro Gly Phe Pro Ile Val Leu His 195 200 205 Gly Gly Ser Gly Ile Pro Asp Glu Gln Ile Gln Ala Ala Ile Lys Leu 210 215 220 Gly Val Ala Lys Val Asn Val Asn Thr Glu Cys Gln Ile Ala Phe Ala 225 230 235 240 Asn Ala Thr Arg Lys Phe Ala Arg Asp Tyr Glu Ala Asn Glu Ala Glu 245 250 255 Tyr Asp Lys Lys Lys Leu Phe Asp Pro Arg Lys Phe Leu Ala Asp Gly 260 265 270 Val Lys Ala Ile Gln Ala Ser Val Glu Glu Arg Ile Asp Val Phe Gly 275 280 285 Ser Glu Gly Lys Ala 290 <210> 49 <211> 837 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (837) <223> Streptococcus pneumoniae spr0585 gene <400> 49 atgacaaaag cagatacgat ttttaaagag aatattgaac gcattctaaa ggaaggtgtt 60 ttctcagagc aggctcgtcc taagtacaag gatgggactg ttgctaattc caagtatgta 120 actggtgcct tttccgagta cgacttatct aaaggagaat ttcctatcac aactttgcgt 180 cccattgcaa tcaaatccgc catcaaagaa gttctctgga tttaccaaga ccagtcaaat 240 agcttagaag tgcttaatga caagtacaat gttcactact ggaatgactg ggaagttgga 300 gacacgggaa ccattggtga gcgctatggt gccgttgtta agaaacacga cattatcaat 360 aagcttctca aacagttgga aaccaatcct tggaaccgcc gcaatattat ttcgctctgg 420 gattaccaag ctttcgaaga aacagatggg ctgctcccgt gcgcctttca gaccatgttt 480 gatgttcggc gtgttgatgg ggaaatctat ctggatgcga ccttgaccca gcgctccaat 540 gatatgctgg tggcccacca catcaacgct atgcagtatg tggctttgca gatgatgatt 600 gccaaacatt ttggctggaa ggttgggaag ttcttctact tcatcaacaa cctccatatc 660 tatgataatc aatttgaaca agctcaggaa ttgctccgtc gggagccgtc aaactgccaa 720 ccacgcttgg ttttaaatgt tcctgatggg actaatttct ttgatatcaa agcagaagat 780 tttgagttgg tggattatga ccctgttaag ccacagttga agtttgacct agctatt 837 <210> 50 <211> 279 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) ... (279) <223> Thymidylate synthase <400> 50 Met Thr Lys Ala Asp Thr Ile Phe Lys Glu Asn Ile Glu Arg Ile Leu 1 5 10 15 Lys Glu Gly Val Phe Ser Glu Gln Ala Arg Pro Lys Tyr Lys Asp Gly 20 25 30 Thr Val Ala Asn Ser Lys Tyr Val Thr Gly Ala Phe Ser Glu Tyr Asp 35 40 45 Leu Ser Lys Gly Glu Phe Pro Ile Thr Thr Leu Arg Pro Ile Ala Ile 50 55 60 Lys Ser Ala Ile Lys Glu Val Leu Trp Ile Tyr Gln Asp Gln Ser Asn 65 70 75 80 Ser Leu Glu Val Leu Asn Asp Lys Tyr Asn Val His Tyr Trp Asn Asp 85 90 95 Trp Glu Val Gly Asp Thr Gly Thr Ile Gly Glu Arg Tyr Gly Ala Val 100 105 110 Val Lys Lys His Asp Ile Ile Asn Lys Leu Leu Lys Gln Leu Glu Thr 115 120 125 Asn Pro Trp Asn Arg Arg Asn Ile Ile Ser Leu Trp Asp Tyr Gln Ala 130 135 140 Phe Glu Glu Thr Asp Gly Leu Leu Pro Cys Ala Phe Gln Thr Met Phe 145 150 155 160 Asp Val Arg Arg Val Asp Gly Glu Ile Tyr Leu Asp Ala Thr Leu Thr 165 170 175 Gln Arg Ser Asn Asp Met Leu Val Ala His His Ile Asn Ala Met Gln 180 185 190 Tyr Val Ala Leu Gln Met Met Ile Ala Lys His Phe Gly Trp Lys Val 195 200 205 Gly Lys Phe Phe Tyr Phe Ile Asn Asn Leu His Ile Tyr Asp Asn Gln 210 215 220 Phe Glu Gln Ala Gln Glu Leu Leu Arg Arg Glu Pro Ser Asn Cys Gln 225 230 235 240 Pro Arg Leu Val Leu Asn Val Pro Asp Gly Thr Asn Phe Phe Asp Ile 245 250 255 Lys Ala Glu Asp Phe Glu Leu Val Asp Tyr Asp Pro Val Lys Pro Gln 260 265 270 Leu Lys Phe Asp Leu Ala Ile 275 <210> 51 <211> 1056 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1056) <223> Streptococcus pneumoniae spr0604 gene <400> 51 atgaaaaaaa ttgtctttac aggtgggggg acggttggac acgttaccct caatcttttg 60 ttaatgccca agttcatcga agatggttgg gaagtccact atatcgggga caagcgtggt 120 atcgaacacc aagaaatcct taagtcaggt ttggatgtca ctttccactc cattgcgact 180 gggaaattgc gtcgctattt ctcttggcaa aatatgctgg acgtcttcaa agttggctgg 240 ggaatcgtcc aatcgctctt tatcatgttg cgacttcgtc cacagaccct tttttcaaag 300 gggggctttg tctcagtacc gcctgttatc gcagcgcgtg tgtcaggagt gcctgtcttt 360 attcacgaat ctgacctgtc tatgggcttg gccaataaaa tcgcctataa atttgcgact 420 aagatgtatt caacctttga gcaagcttcg agtttgtcta aggttgagca tgtgggagca 480 gtgaccaagg tttcagatca aaaaaatcca gaaccagatg aattggtgga tattcaaacc 540 cactttaatc ataaattgcc gactgtattg tttgttggcg gttctgcagg tgctcgtgtc 600 tttaaccaat tggtgacaga ccataagaaa gaactaacag agcgctacaa tattatcaat 660 ctaactggag attctagtct gaacgagttg agccaaaatc tttttcgtgt tgactatgtg 720 accgatctct atcaaccctt gatggaattg gctgatattg ttgtgacacg aggtggtgcc 780 aatacgattt ttgagctctt ggcgatagca aaattgcatg tcattgtgcc gcttggtcgt 840 gaagctagtc gtggtgacca gattgaaaat gcagcttact ttgtaaaaaa aggctatgca 900 gaagaccttc aagaaagcga tttgaccttg gatagtttgg aagagaagct ttctcactta 960 ctaagtcaca aggaagatta ccaagctaag atgaaagctt ctaaggaatt gaaatctcta 1020 gcagattttt atcaattgtt gaaaaaagat ttatca 1056 <210> 52 <211> 352 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (352) <223> Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase <400> 52 Met Lys Lys Ile Val Phe Thr Gly Gly Gly Thr Val Gly His Val Thr 1 5 10 15 Leu Asn Leu Leu Leu Met Pro Lys Phe Ile Glu Asp Gly Trp Glu Val 20 25 30 His Tyr Ile Gly Asp Lys Arg Gly Ile Glu His Gln Glu Ile Leu Lys 35 40 45 Ser Gly Leu Asp Val Thr Phe His Ser Ile Ala Thr Gly Lys Leu Arg 50 55 60 Arg Tyr Phe Ser Trp Gln Asn Met Leu Asp Val Phe Lys Val Gly Trp 65 70 75 80 Gly Ile Val Gln Ser Leu Phe Ile Met Leu Arg Leu Arg Pro Gln Thr 85 90 95 Leu Phe Ser Lys Gly Gly Phe Val Ser Val Pro Pro Val Ile Ala Ala 100 105 110 Arg Val Ser Gly Val Pro Val Phe Ile His Glu Ser Asp Leu Ser Met 115 120 125 Gly Leu Ala Asn Lys Ile Ala Tyr Lys Phe Ala Thr Lys Met Tyr Ser 130 135 140 Thr Phe Glu Gln Ala Ser Ser Leu Ser Lys Val Glu His Val Gly Ala 145 150 155 160 Val Thr Lys Val Ser Asp Gln Lys Asn Pro Glu Pro Asp Glu Leu Val 165 170 175 Asp Ile Gln Thr His Phe Asn His Lys Leu Pro Thr Val Leu Phe Val 180 185 190 Gly Gly Ser Ala Gly Ala Arg Val Phe Asn Gln Leu Val Thr Asp His 195 200 205 Lys Lys Glu Leu Thr Glu Arg Tyr Asn Ile Ile Asn Leu Thr Gly Asp 210 215 220 Ser Ser Leu Asn Glu Leu Ser Gln Asn Leu Phe Arg Val Asp Tyr Val 225 230 235 240 Thr Asp Leu Tyr Gln Pro Leu Met Glu Leu Ala Asp Ile Val Val Thr 245 250 255 Arg Gly Gly Ala Asn Thr Ile Phe Glu Leu Leu Ala Ile Ala Lys Leu 260 265 270 His Val Ile Val Pro Leu Gly Arg Glu Ala Ser Arg Gly Asp Gln Ile 275 280 285 Glu Asn Ala Ala Tyr Phe Val Lys Lys Gly Tyr Ala Glu Asp Leu Gln 290 295 300 Glu Ser Asp Leu Thr Leu Asp Ser Leu Glu Glu Lys Leu Ser His Leu 305 310 315 320 Leu Ser His Lys Glu Asp Tyr Gln Ala Lys Met Lys Ala Ser Lys Glu 325 330 335 Leu Lys Ser Leu Ala Asp Phe Tyr Gln Leu Leu Lys Lys Lys Asp Leu Ser 340 345 350 <210> 53 <211> 1488 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1488) <223> Streptococcus pneumoniae spr0626 gene <400> 53 atgtctacag aacatatgga agaactaaat gaccagcaga tcgttcgccg tgaaaaaatg 60 gctgcgctcc gcgaacaagg aatcgatcct ttcggaaaac gttttgaacg tactgcaaat 120 tcacaagaat taaaagataa atatgccaac ctcgataaag aacaattaca cgataaaaac 180 gaaacagcta ctatcgcagg acgcttggta accaaacgtg gtaaaggaaa agttggtttt 240 gcccaccttc aagaccgcga aggccagatt cagatctatg ttcgtaagga tgctgtcggt 300 gaagaaaact acgaaatctt caaaaaagca gaccttggtg acttccttgg tgtcgaaggt 360 gaagtgatgc gtacggatat gggagaactc tctatcaagg caacccacat cacacacttg 420 tctaaggctc ttcgtcctct tcctgagaaa ttccatggtt tgacagacgt tgaaacaatt 480 taccgtaaac gttaccttga cttgatttct aatcgtgaaa gctttgaacg ctttgtcact 540 cgttcaaaaa tcatctctga aatccgtcgt taccttgacc aaaaaggatt ccttgaagtg 600 gaaacacctg ttcttcataa tgaagccggt ggagctgctg cccgtccatt tatcacccac 660 cacaatgccc aaaacattga catggtgctt cgtatcgcga ctgagcttca cttaaaacgc 720 cttatcgtgg gtggtatgga acgtgtctat gaaattggcc gtatcttccg taacgaagga 780 atggatgcta ctcataaccc tgagttcact tctatcgaag tttaccaagc ctatgcagac 840 ttccaagata tcatggactt gacggaaggc attatccaac atgctgctaa atcagtcaaa 900 ggtgatggcc cagtcaacta ccaaggtact gaaatcaaga ttaacgaacc atttaagcgt 960 gttcatatgg tggatgctat cagagaaatt actggtgtcg atttctggca agacatgact 1020 ttggaagaag ctaaagctat cgctgctgag aagaaagttc cagttgagaa acactacact 1080 gaggttggtc acatcatcaa tgccttcttt gaagagtttg ttgaagaaac tttaatccaa 1140 ccaacctttg tctatggaca tccagtagct gtatctccac tcgctaagaa aaatcctgaa 1200 gaccaacgct ttactgaccg tttcgagctc tttatcatga ctaaggagta cggtaatgcc 1260 tttactgagc tcaacgaccc aatcgaccaa cttagccgtt ttgaagccca agctaaagcc 1320 aaagaacttg gtgatgatga agcgacagga atcgactatg actacattga agcccttgaa 1380 tacggtatgc caccaacagg tggtttggga atcggtatcg accgtctctg catgctcctc 1440 actgatacaa caactatccg tgatgtattg ctcttcccaa caatgaaa 1488 <210> 54 <211> 496 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (496) Lysyl-tRNA synthetase (lysine-tRNA ligase) <400> 54 Met Ser Thr Glu His Met Glu Glu Leu Asn Asp Gln Gln Ile Val Arg 1 5 10 15 Arg Glu Lys Met Ala Ala Leu Arg Glu Gln Gly Ile Asp Pro Phe Gly 20 25 30 Lys Arg Phe Glu Arg Thr Ala Asn Ser Gln Glu Leu Lys Asp Lys Tyr 35 40 45 Ala Asn Leu Asp Lys Glu Gln Leu His Asp Lys Asn Glu Thr Ala Thr 50 55 60 Ile Ala Gly Arg Leu Val Thr Lys Arg Gly Lys Gly Lys Val Gly Phe 65 70 75 80 Ala His Leu Gln Asp Arg Glu Gly Gln Ile Gln Ile Tyr Val Arg Lys 85 90 95 Asp Ala Val Gly Glu Glu Asn Tyr Glu Ile Phe Lys Lys Ala Asp Leu 100 105 110 Gly Asp Phe Leu Gly Val Glu Gly Glu Val Met Arg Thr Asp Met Gly 115 120 125 Glu Leu Ser Ile Lys Ala Thr His Ile Thr His Leu Ser Lys Ala Leu 130 135 140 Arg Pro Leu Pro Glu Lys Phe His Gly Leu Thr Asp Val Glu Thr Ile 145 150 155 160 Tyr Arg Lys Arg Tyr Leu Asp Leu Ile Ser Asn Arg Glu Ser Phe Glu 165 170 175 Arg Phe Val Thr Arg Ser Lys Ile Ile Ser Glu Ile Arg Arg Tyr Leu 180 185 190 Asp Gln Lys Gly Phe Leu Glu Val Glu Thr Pro Val Leu His Asn Glu 195 200 205 Ala Gly Gly Ala Ala Ala Arg Pro Phe Ile Thr His His Asn Ala Gln 210 215 220 Asn Ile Asp Met Val Leu Arg Ile Ala Thr Glu Leu His Leu Lys Arg 225 230 235 240 Leu Ile Val Gly Gly Met Glu Arg Val Tyr Glu Ile Gly Arg Ile Phe 245 250 255 Arg Asn Glu Gly Met Asp Ala Thr His Asn Pro Glu Phe Thr Ser Ile 260 265 270 Glu Val Tyr Gln Ala Tyr Ala Asp Phe Gln Asp Ile Met Asp Leu Thr 275 280 285 Glu Gly Ile Ile Gln His Ala Ala Lys Ser Val Lys Gly Asp Gly Pro 290 295 300 Val Asn Tyr Gln Gly Thr Glu Ile Lys Ile Asn Glu Pro Phe Lys Arg 305 310 315 320 Val His Met Val Asp Ala Ile Arg Glu Ile Thr Gly Val Asp Phe Trp 325 330 335 Gln Asp Met Thr Leu Glu Glu Ala Lys Ala Ile Ala Ala Glu Lys Lys 340 345 350 Val Pro Val Glu Lys His Tyr Thr Glu Val Gly His Ile Ile Asn Ala 355 360 365 Phe Phe Glu Glu Phe Val Glu Glu Thr Leu Ile Gln Pro Thr Phe Val 370 375 380 Tyr Gly His Pro Val Ala Val Ser Pro Leu Ala Lys Lys Asn Pro Glu 385 390 395 400 Asp Gln Arg Phe Thr Asp Arg Phe Glu Leu Phe Ile Met Thr Lys Glu 405 410 415 Tyr Gly Asn Ala Phe Thr Glu Leu Asn Asp Pro Ile Asp Gln Leu Ser 420 425 430 Arg Phe Glu Ala Gln Ala Lys Ala Lys Glu Leu Gly Asp Asp Glu Ala 435 440 445 Thr Gly Ile Asp Tyr Asp Tyr Ile Glu Ala Leu Glu Tyr Gly Met Pro 450 455 460 Pro Thr Gly Gly Leu Gly Ile Gly Ile Asp Arg Leu Cys Met Leu Leu 465 470 475 480 Thr Asp Thr Thr Thr Ile Arg Asp Val Leu Leu Phe Pro Thr Met Lys 485 490 495 <210> 55 <211> 294 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) ... (294) <223> Streptococcus pneumoniae spr0646 gene <400> 55 atggatacac cggatgagaa tggttatgta gcagatgact atcggattac ttacttagag 60 gcccacatca aggccatgcg agatgccatt tacaaagacg gggttgactt gcttggttat 120 acgacatggg gctgtatcga ttcggtttca gctggcacag gggaaatgaa taagcgctat 180 ggctttatct atgtagatcg agataatgta ggaaacggag ctctcaaacg tagcaagaag 240 aagtccttct attggtacaa ggatgtcatt gatagcaatg gtgtaagcat cgag 294 <210> 56 <211> 98 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (98) <223> Phospho-beta-gluco or galactosidase <400> 56 Met Asp Thr Pro Asp Glu Asn Gly Tyr Val Ala Asp Asp Tyr Arg Ile 1 5 10 15 Thr Tyr Leu Glu Ala His Ile Lys Ala Met Arg Asp Ala Ile Tyr Lys 20 25 30 Asp Gly Val Asp Leu Leu Gly Tyr Thr Thr Trp Gly Cys Ile Asp Ser 35 40 45 Val Ser Ala Gly Thr Gly Glu Met Asn Lys Arg Tyr Gly Phe Ile Tyr 50 55 60 Val Asp Arg Asp Asn Val Gly Asn Gly Ala Leu Lys Arg Ser Lys Lys 65 70 75 80 Lys Ser Phe Tyr Trp Tyr Lys Asp Val Ile Asp Ser Asn Gly Val Ser 85 90 95 Ile glu <210> 57 <211> 690 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (690) <223> Streptococcus pneumoniae spr0666 gene <400> 57 atgtcaatta ttgaaatgag agatgtcgtt aaaaaatacg acaacggaac gactgctcta 60 cgcggtgttt cggttagcgt tcaaccgggg gaatttgctt acatcgtagg accttcagga 120 gcagggaagt caacttttat tcgttctctg tatcgtgaag taaaaatcga taaaggaagt 180 ctatcagttg ctggttttaa tctggttaag atcaaaaaga aagatgtccc gcttctacgt 240 cgtagtgttg gggttgtctt ccaagattat aaattgttac caaagaaaac tgtctatgaa 300 aatattgctt acgctatgga agtaatcggg gaaaatcgcc gtaatatcaa aagacgagtg 360 atggaagttt tggacttggt tggattgaag cataaggttc gttctttccc aaatgaactc 420 tcaggtgggg agcaacagcg gattgcgatt gcgcgtgcaa ttgtaaataa tcccaaagta 480 ttgatagctg atgagccaac aggaaatctg gatccggata attcatggga aattatgaat 540 ctcttggaac ggattaacct acaaggaaca actattttga tggcgactca taatagccag 600 attgtaaata ccttgcgcca ccgtgtcatt gccattgaaa atggccgtgt cgttcgtgac 660 gaatcaaaag gagagtatgg atacgatgat 690 <210> 58 <211> 230 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (230) <223> ABC transporter ATP-binding protein & cell division protein <400> 58 Met Ser Ile Ile Glu Met Arg Asp Val Val Lys Lys Tyr Asp Asn Gly 1 5 10 15 Thr Thr Ala Leu Arg Gly Val Ser Val Ser Val Gln Pro Gly Glu Phe 20 25 30 Ala Tyr Ile Val Gly Pro Ser Gly Ala Gly Lys Ser Thr Phe Ile Arg 35 40 45 Ser Leu Tyr Arg Glu Val Lys Ile Asp Lys Gly Ser Leu Ser Val Ala 50 55 60 Gly Phe Asn Leu Val Lys Ile Lys Lys Lys Asp Val Pro Leu Leu Arg 65 70 75 80 Arg Ser Val Gly Val Val Phe Gln Asp Tyr Lys Leu Leu Pro Lys Lys 85 90 95 Thr Val Tyr Glu Asn Ile Ala Tyr Ala Met Glu Val Ile Gly Glu Asn 100 105 110 Arg Arg Asn Ile Lys Arg Arg Val Met Glu Val Leu Asp Leu Val Gly 115 120 125 Leu Lys His Lys Val Arg Ser Phe Pro Asn Glu Leu Ser Gly Gly Glu 130 135 140 Gln Gln Arg Ile Ala Ile Ala Arg Ala Ile Val Asn Asn Pro Lys Val 145 150 155 160 Leu Ile Ala Asp Glu Pro Thr Gly Asn Leu Asp Pro Asp Asn Ser Trp 165 170 175 Glu Ile Met Asn Leu Leu Glu Arg Ile Asn Leu Gln Gly Thr Thr Ile 180 185 190 Leu Met Ala Thr His Asn Ser Gln Ile Val Asn Thr Leu Arg His Arg 195 200 205 Val Ile Ala Ile Glu Asn Gly Arg Val Val Arg Asp Glu Ser Lys Gly 210 215 220 Glu Tyr Gly Tyr Asp Asp 225 230 <210> 59 <211> 1341 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1341) <223> Streptococcus pneumoniae spr0670 gene <400> 59 atgtcattta cgaaatttca atttaaaaac tatattagag aagccttgaa ggagttaaaa 60 tttacaactc caacagaggt gcaagacaag ttgattccta ttgttttggc aggtcgtgac 120 ctagtaggag aatcaaaaac aggttcaggt aagactcaca ctttcttgtt accgattttc 180 cagcaattag atgaagctag cgatagtgta caagcagtga ttactgcacc gagtcgtgag 240 ttggctactc aaatttacca agtagcgcgt cagatttcag ctcactcaga tgtcgaagtt 300 cgtgtggtta attatgtggg tggtacggat aaggctcgcc agattgagaa attggaaagc 360 aatcagcctc atattgttat tggaacacca ggccgtatct acgacttggt taaatctggt 420 gatttagcta ttcataaagc caagacattt gttgttgatg aagcagatat gaccttggat 480 atgggattct tggaaactgt tgataagatt gctggcagtc ttccaaaaga cttgcaattc 540 atggtcttct cagcgactat cccacaaaaa ctgcaaccat tcttgaaaaa atacttatca 600 aatcctgtta tggagaaaat taagactaaa acggttattt ctgacaccat tgataattgg 660 ttgatttcga ccaagggaca tgataagaat gctcaaattt accagttgac tcagttgatg 720 cagccgtatt tggcaatgat ttttgttaac actaaaacgc gtgctgatga attgcattca 780 tatctgactg ctcaaggctt gaaggttgca aaaatccatg gcgatattgc ccctcgtgaa 840 cgcaagcgaa tcatgaatca ggtgcaaaat ctggattttg agtatattgt cgcaacagat 900 ttagcagcgc gtgggattga cattgaaggt gtcagccatg tcatcaatga tgccattccg 960 caagacttat ctttttttgt tcaccgtgtt ggtcgtactg gacgaaatgg cctaccaggt 1020 acagctatta ccctttatca gccaagtgat gactcggata tccgtgagtt ggagaaattg 1080 ggaatcaagt ttagtcctaa gatggtcaaa gatggggaat ttcaagatac ctatgaccgt 1140 gatcgtcgtg ccaaccgtga gaaaaaacaa gataaacttg atatcgaaat gattggtttg 1200 gttaaaaaga aaaagaaaaa agtcaaaccg ggttataaga agaaaattca atgggcggtt 1260 gatgaaaagc gccgtaaaac taagcgtgct gaaaatcgcg ctcgcggtcg tgcagagcgt 1320 aaagctaaac gccaaacatt t 1341 <210> 60 <211> 447 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (447) <223> ATP-dependent RNA helicase <400> 60 Met Ser Phe Thr Lys Phe Gln Phe Lys Asn Tyr Ile Arg Glu Ala Leu 1 5 10 15 Lys Glu Leu Lys Phe Thr Thr Pro Thr Glu Val Gln Asp Lys Leu Ile 20 25 30 Pro Ile Val Leu Ala Gly Arg Asp Leu Val Gly Glu Ser Lys Thr Gly 35 40 45 Ser Gly Lys Thr His Thr Phe Leu Leu Pro Ile Phe Gln Gln Leu Asp 50 55 60 Glu Ala Ser Asp Ser Val Gln Ala Val Ile Thr Ala Pro Ser Arg Glu 65 70 75 80 Leu Ala Thr Gln Ile Tyr Gln Val Ala Arg Gln Ile Ser Ala His Ser 85 90 95 Asp Val Glu Val Arg Val Val Asn Tyr Val Gly Gly Thr Asp Lys Ala 100 105 110 Arg Gln Ile Glu Lys Leu Glu Ser Asn Gln Pro His Ile Val Ile Gly 115 120 125 Thr Pro Gly Arg Ile Tyr Asp Leu Val Lys Ser Gly Asp Leu Ala Ile 130 135 140 His Lys Ala Lys Thr Phe Val Val Asp Glu Ala Asp Met Thr Leu Asp 145 150 155 160 Met Gly Phe Leu Glu Thr Val Asp Lys Ile Ala Gly Ser Leu Pro Lys 165 170 175 Asp Leu Gln Phe Met Val Phe Ser Ala Thr Ile Pro Gln Lys Leu Gln 180 185 190 Pro Phe Leu Lys Lys Tyr Leu Ser Asn Pro Val Met Glu Lys Ile Lys 195 200 205 Thr Lys Thr Val Ile Ser Asp Thr Ile Asp Asn Trp Leu Ile Ser Thr 210 215 220 Lys Gly His Asp Lys Asn Ala Gln Ile Tyr Gln Leu Thr Gln Leu Met 225 230 235 240 Gln Pro Tyr Leu Ala Met Ile Phe Val Asn Thr Lys Thr Arg Ala Asp 245 250 255 Glu Leu His Ser Tyr Leu Thr Ala Gln Gly Leu Lys Val Ala Lys Ile 260 265 270 His Gly Asp Ile Ala Pro Arg Glu Arg Lys Arg Ile Met Asn Gln Val 275 280 285 Gln Asn Leu Asp Phe Glu Tyr Ile Val Ala Thr Asp Leu Ala Ala Arg 290 295 300 Gly Ile Asp Ile Glu Gly Val Ser His Val Ile Asn Asp Ala Ile Pro 305 310 315 320 Gln Asp Leu Ser Phe Phe Val His Arg Val Gly Arg Thr Gly Arg Asn 325 330 335 Gly Leu Pro Gly Thr Ala Ile Thr Leu Tyr Gln Pro Ser Asp Asp Ser 340 345 350 Asp Ile Arg Glu Leu Glu Lys Leu Gly Ile Lys Phe Ser Pro Lys Met 355 360 365 Val Lys Asp Gly Glu Phe Gln Asp Thr Tyr Asp Arg Asp Arg Arg Ala 370 375 380 Asn Arg Glu Lys Lys Lys Gln Asp Lys Leu Asp Ile Glu Met Ile Gly Leu 385 390 395 400 Val Lys Lys Lys Lys Lys Lys Val Lys Pro Gly Tyr Lys Lys Lys Ile 405 410 415 Gln Trp Ala Val Asp Glu Lys Arg Arg Lys Thr Lys Arg Ala Glu Asn 420 425 430 Arg Ala Arg Gly Arg Ala Glu Arg Lys Ala Lys Arg Gln Thr Phe 435 440 445 <210> 61 <211> 1188 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1188) <223> Streptococcus pneumoniae spr0671 gene <400> 61 atgtcagaac gtaaattatt cacgtctgaa tctgtatctg aggggcatcc ggataagatt 60 gcagaccaaa tttcagatgc gattttggat gctattttag caaaggatcc agaggcgcac 120 gttgctgctg aaacagctgt atatactggt tctgtccacg tttttggtga aatttctaca 180 aatgcctatg tggatattaa ccgtgtggtt cgtgatacca ttgcagagat tggttatacc 240 aatacagaat atggattttc tgctgagacg gtgggagtac acccatcttt ggtggaacaa 300 tctcctgaca tcgctcaagg tgttaacgaa gccttggagg ttcgcggaaa tgctgatcaa 360 gatccactgg acttgattgg agcaggtgac caagggctca tgtttggatt tgcagtagat 420 gaaacagaag agcttatgcc attgccaatt gcactcagtc ataaattggt tcgtcgtctg 480 gcagaacttc gtaagtctgg agaaattagc tatctccgtc cagatgcaaa atcacaagtt 540 acagttgagt acgatgaaaa tgaccgtccg gtacgtgtag atacagtcgt tatttctact 600 cagcatgatc cagaggccac taatgaacaa atccatcaag atgtgattga caaggtcatc 660 aaagaagtta ttccatcttc ttatcttgat gataagacaa aattctttat caatccgaca 720 ggtcgttttg taatcggtgg tcctcaaggg gactcaggtt tgactggtcg taagattatt 780 gtagatactt atggtggcta ctctcgtcat ggtggtggtg ccttctctgg taaagatgcg 840 actaaggtgg atcgttcagc ctcttatgcg gctcgctata ttgccaagaa tatcgttgca 900 gcaggccttg ctaagaaggc agaagtgcag ttggcctatg ctatcggtgt tgcgcaacct 960 gtttctgttc gtatcgatac tttcggtaca ggaacagtag ctgaaagtca acttgaaaaa 1020 gcggctcgtc aaatctttga ccttcgccct gcagggatta tccaaatgct ggacctcaag 1080 cgtccaattt accgtcaaac atcggcttac ggtcacatgg gacgtacaga tattgatctt 1140 ccatgggaac gtttggataa ggtagatgct ttgaaagaag cagtaaaa 1188 <210> 62 <211> 396 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (396) <223> S-adenosylmethionine synthetase <400> 62 Met Ser Glu Arg Lys Leu Phe Thr Ser Glu Ser Val Ser Glu Gly His 1 5 10 15 Pro Asp Lys Ile Ala Asp Gln Ile Ser Asp Ala Ile Leu Asp Ala Ile 20 25 30 Leu Ala Lys Asp Pro Glu Ala His Val Ala Ala Glu Thr Ala Val Tyr 35 40 45 Thr Gly Ser Val His Val Phe Gly Glu Ile Ser Thr Asn Ala Tyr Val 50 55 60 Asp Ile Asn Arg Val Val Arg Asp Thr Ile Ala Glu Ile Gly Tyr Thr 65 70 75 80 Asn Thr Glu Tyr Gly Phe Ser Ala Glu Thr Val Gly Val His Pro Ser 85 90 95 Leu Val Glu Gln Ser Pro Asp Ile Ala Gln Gly Val Asn Glu Ala Leu 100 105 110 Glu Val Arg Gly Asn Ala Asp Gln Asp Pro Leu Asp Leu Ile Gly Ala 115 120 125 Gly Asp Gln Gly Leu Met Phe Gly Phe Ala Val Asp Glu Thr Glu Glu 130 135 140 Leu Met Pro Leu Pro Ile Ala Leu Ser His Lys Leu Val Arg Arg Leu 145 150 155 160 Ala Glu Leu Arg Lys Ser Gly Glu Ile Ser Tyr Leu Arg Pro Asp Ala 165 170 175 Lys Ser Gln Val Thr Val Glu Tyr Asp Glu Asn Asp Arg Pro Val Arg 180 185 190 Val Asp Thr Val Val Ile Ser Thr Gln His Asp Pro Glu Ala Thr Asn 195 200 205 Glu Gln Ile His Gln Asp Val Ile Asp Lys Val Ile Lys Glu Val Ile 210 215 220 Pro Ser Ser Tyr Leu Asp Asp Lys Thr Lys Phe Phe Ile Asn Pro Thr 225 230 235 240 Gly Arg Phe Val Ile Gly Gly Pro Gln Gly Asp Ser Gly Leu Thr Gly 245 250 255 Arg Lys Ile Ile Val Asp Thr Tyr Gly Gly Tyr Ser Arg His Gly Gly 260 265 270 Gly Ala Phe Ser Gly Lys Asp Ala Thr Lys Val Asp Arg Ser Ala Ser 275 280 285 Tyr Ala Ala Arg Tyr Ile Ala Lys Asn Ile Val Ala Ala Gly Leu Ala 290 295 300 Lys Lys Ala Glu Val Gln Leu Ala Tyr Ala Ile Gly Val Ala Gln Pro 305 310 315 320 Val Ser Val Arg Ile Asp Thr Phe Gly Thr Gly Thr Val Ala Glu Ser 325 330 335 Gln Leu Glu Lys Ala Ala Arg Gln Ile Phe Asp Leu Arg Pro Ala Gly 340 345 350 Ile Ile Gln Met Leu Asp Leu Lys Arg Pro Ile Tyr Arg Gln Thr Ser 355 360 365 Ala Tyr Gly His Met Gly Arg Thr Asp Ile Asp Leu Pro Trp Glu Arg 370 375 380 Leu Asp Lys Val Asp Ala Leu Lys Glu Ala Val Lys 385 390 395 <210> 63 <211> 918 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (918) <223> Streptococcus pneumoniae spr0729 gene <400> 63 gtgttagagg gcactagcag tttttggtat aatggaagat atttgaaaga aaagagaagt 60 gatatgacac agattattga tgggaaagct ttagcggcca aattgcaggg gcagttggct 120 gaaaagactg caaaattaaa ggaagaaaca gctctagtgc ctggtttggt agtgattttg 180 gttggggata atccagccag ccaagtctac gttcgcaaca aggagaggtc agctcttgcg 240 gctggttccc gtagcgaagt agtgcgagtt ccagagacca ttactcaaga ggaattgtta 300 gacctgattg ctaaatataa tcaggatcca gcttggcatg ggattttggt ccagttgcca 360 ttaccaaaac atatcgatga agaggcggtt ttattagcca ttgacccaga aaaggatgtg 420 gatggtttcc atcccctaaa catgggacgt ctttggtctg gacatccagt catgattccc 480 tcgacacctg caggaattat ggaaatgttt catgaatatg ggattgactt ggaaggtaaa 540 aatgcggtcg taatcggtcg ttcaaatatc gttggaaaac ctatggctca gcttcttttg 600 gctaaaaatg cgacagtaac cttgacccac tcacgaactc ataatcttgc caaggtggct 660 gctaaagcag atattcttgt agtcgcaatc ggccgcgcca agtttgtgac tgctgacttt 720 gtcaaaccag gtgcggttgt cattgacgtt gggatgaatc gagatgaaaa tgggaagctc 780 tgtggagatg ttgattatga ggcagttgca ccacttgcta gccatatcac gccagtccct 840 ggaggtgtcg gtcctatgac cattaccatg ctgatggagc aaacttatca agcagcactt 900 cggacattgg atagaaaa 918 <210> 64 <211> 306 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (306) <223> methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrolase <400> 64 Met Leu Glu Gly Thr Ser Ser Phe Trp Tyr Asn Gly Arg Tyr Leu Lys 1 5 10 15 Glu Lys Arg Ser Asp Met Thr Gln Ile Ile Asp Gly Lys Ala Leu Ala 20 25 30 Ala Lys Leu Gln Gly Gln Leu Ala Glu Lys Thr Ala Lys Leu Lys Glu 35 40 45 Glu Thr Ala Leu Val Pro Gly Leu Val Val Ile Leu Val Gly Asp Asn 50 55 60 Pro Ala Ser Gln Val Tyr Val Arg Asn Lys Glu Arg Ser Ala Leu Ala 65 70 75 80 Ala Gly Ser Arg Ser Glu Val Val Arg Val Pro Glu Thr Ile Thr Gln 85 90 95 Glu Glu Leu Leu Asp Leu Ile Ala Lys Tyr Asn Gln Asp Pro Ala Trp 100 105 110 His Gly Ile Leu Val Gln Leu Pro Leu Pro Lys His Ile Asp Glu Glu 115 120 125 Ala Val Leu Leu Ala Ile Asp Pro Glu Lys Asp Val Asp Gly Phe His 130 135 140 Pro Leu Asn Met Gly Arg Leu Trp Ser Gly His Pro Val Met Ile Pro 145 150 155 160 Ser Thr Pro Ala Gly Ile Met Glu Met Phe His Glu Tyr Gly Ile Asp 165 170 175 Leu Glu Gly Lys Asn Ala Val Val Ile Gly Arg Ser Asn Ile Val Gly 180 185 190 Lys Pro Met Ala Gln Leu Leu Leu Ala Lys Asn Ala Thr Val Thr Leu 195 200 205 Thr His Ser Arg Thr His Asn Leu Ala Lys Val Ala Ala Lys Ala Asp 210 215 220 Ile Leu Val Val Ala Ile Gly Arg Ala Lys Phe Val Thr Ala Asp Phe 225 230 235 240 Val Lys Pro Gly Ala Val Val Ile Asp Val Gly Met Asn Arg Asp Glu 245 250 255 Asn Gly Lys Leu Cys Gly Asp Val Asp Tyr Glu Ala Val Ala Pro Leu 260 265 270 Ala Ser His Ile Thr Pro Val Pro Gly Gly Val Gly Pro Met Thr Ile 275 280 285 Thr Met Leu Met Glu Gln Thr Tyr Gln Ala Ala Leu Arg Thr Leu Asp 290 295 300 Arg lys 305 <210> 65 <211> 639 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (639) <223> Streptococcus pneumoniae spr0755 gene <400> 65 atgattacaa tagttttatt aatcctagcc tatctgctgg gttcgattcc atctggtctc 60 tggattggac aagtattctt tcaaatcaat ctacgcgagc atggttctgg taacactgga 120 acgaccaaca ccttccgcat tttaggtaag aaagctggta tggcaacctt tgtgattgac 180 tttttcaaag gaaccctagc aacgctgctt ccgattattt ttcatctaca aggcgtttct 240 cctctcatct ttggactttt ggctgttatc ggccatacct tccctatctt tgcaggattt 300 aaaggtggta aggctgtcgc aaccagtgct ggagtgattt tcggatttgc gcctatcttc 360 tgtctctacc ttgcgattat cttctttgga gctctctatc ttggcagtat gatttcactg 420 tctagtgtca cagcatcgat cgcggctgtt atcggggttc tgctctttcc actttttggt 480 tttatcctga gtaactatga ctctctcttc atcgctatta tcttagcact tgctagtttg 540 attatcattc gtcataagga caatatagct cgtatcaaaa ataaaactga aaatttggtc 600 ccttggggat tgaacctaac ccatcaagat cctaaaaaa 639 <210> 66 <211> 213 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (213) <223> Conserved hypothetical protein <400> 66 Met Ile Thr Ile Val Leu Leu Ile Leu Ala Tyr Leu Leu Gly Ser Ile 1 5 10 15 Pro Ser Gly Leu Trp Ile Gly Gln Val Phe Phe Gln Ile Asn Leu Arg 20 25 30 Glu His Gly Ser Gly Asn Thr Gly Thr Thr Asn Thr Phe Arg Ile Leu 35 40 45 Gly Lys Lys Ala Gly Met Ala Thr Phe Val Ile Asp Phe Phe Lys Gly 50 55 60 Thr Leu Ala Thr Leu Leu Pro Ile Ile Phe His Leu Gln Gly Val Ser 65 70 75 80 Pro Leu Ile Phe Gly Leu Leu Ala Val Ile Gly His Thr Phe Pro Ile 85 90 95 Phe Ala Gly Phe Lys Gly Gly Lys Ala Val Ala Thr Ser Ala Gly Val 100 105 110 Ile Phe Gly Phe Ala Pro Ile Phe Cys Leu Tyr Leu Ala Ile Ile Phe 115 120 125 Phe Gly Ala Leu Tyr Leu Gly Ser Met Ile Ser Leu Ser Ser Val Thr 130 135 140 Ala Ser Ile Ala Ala Val Ile Gly Val Leu Leu Phe Pro Leu Phe Gly 145 150 155 160 Phe Ile Leu Ser Asn Tyr Asp Ser Leu Phe Ile Ala Ile Ile Leu Ala 165 170 175 Leu Ala Ser Leu Ile Ile Ile Arg His Lys Asp Asn Ile Ala Arg Ile 180 185 190 Lys Asn Lys Thr Glu Asn Leu Val Pro Trp Gly Leu Asn Leu Thr His 195 200 205 Gln Asp Pro Lys Lys 210 <210> 67 <211> 1941 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1941) <223> Streptococcus pneumoniae spr0756 gene <400> 67 gtgtcaaaaa aggaaatcaa tattaacaat tataatgatg atgctattca ggtgctagaa 60 gggttggatg cggtccgaaa acgtccaggg atgtatattg gatcgaccga tggcgctggt 120 cttcatcacc tagtttggga aatcgttgat aatgcagtcg atgaagcctt gtctgggttt 180 ggtgatcgta ttgatgtaac tatcaataaa gacggtagtc taacggttca agaccatgga 240 cgtgggatgc cgacaggtat gcacgctatg ggaattccaa ctgttgaggt tatctttacc 300 attcttcatg ccggagggaa attcggtcaa ggtggctata agacatcagg tggacttcac 360 ggagtgggtt cttccgttgt taacgccctt tctagctggt tagaagttga aattacccgt 420 gatggcgcag tttacaagca acgttttgaa aatggtggaa aacctgtcac gactttgaag 480 aaaatcggta cagcactcaa gtctaaaaca ggcaccaaag ttacttttat gcctgacgcg 540 actatctttt ctacgacaga tttcaagtac aataccattt cagagcgcct taatgaatca 600 gcctttctct tgaaaaatgt gaccttgtct ttaacggaca agcgaacaga tgaagcgatt 660 gagttccact atgagaatgg agtacaagat tttgtttctt atctcaacga agataaggaa 720 atcttgacgc cagttcttta ctttgaaggg gaagacaatg gttttcaagt ggaagtagcc 780 ctccagtaca atgacggatt ctcagataac attctatcct ttgtcaataa cgttcgcacc 840 aaggacggtg gaacgcacga gacaggactc aagtctgcca ttaccaaggt catgaatgac 900 tatgcacgta aaacaggtct tctcaaggaa aaagataaaa accttgaagg ttcagactat 960 cgtgagggac tagcggccgt tctttctatc ttagttcctg aagaacactt gcagtttgaa 1020 ggacagacca aggataaact aggaagcccc ctagctcgcc cagttgtgga tggaatagtg 1080 gctgataagt tgaccttttt ccttatggaa aatggggaat tagcttctaa cctcatccgc 1140 aaggctatca aggcccgtga tgctcgtgaa gcagcacgta aggcgcgtga tgagagccga 1200 aatgggaaga aaaacaagaa agataagggc ttgttgtctg ggaaattgac cccagcccaa 1260 tctaagaatc ctgctaagaa tgaactctat ctagttgagg gggactctgc cggtggttct 1320 gccaaacaag gtcgtgaccg caagttccag gctattctac ctcttcgtgg taaggttatc 1380 aatacagcca aggccaagat ggcggatatc ctcaaaaatg aagagatcaa taccatgatt 1440 tataccattg gtgcgggtgt tggagcagac ttctctattg aagatgccaa ctatgataag 1500 atcattatca tgaccgatgc ggataccgac ggtgcccata tccagacctt gctcttgaca 1560 tttttctacc gttacatgcg tccgctagtc gaggcaggtc atgtctatat tgccctccca 1620 cctctttaca agatgtccaa aggtaaaggc aagaaagaag aagtggccta cgcttggacg 1680 gacggagaac tagaagaact ccgtaaacag ttcggtaaag gcgctaccct ccaacgatac 1740 aaaggacttg gtgagatgaa tgcggaccag ctctgggaaa caaccatgaa cccagaaaca 1800 cgtaccctca tccgtgtcac aattgaagat ttagcgcgcg ccgaacgccg cgtcaatgtt 1860 ctcatgggag ataaggtaga accacgccgt aaatggattg aagataatgt caagtttacg 1920 ctagaagaag cgacagtgtt t 1941 <210> 68 <211> 647 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (647) <223> Topoisomerase IV subunit B <400> 68 Met Ser Lys Lys Glu Ile Asn Ile Asn Asn Tyr Asn Asp Asp Ala Ile 1 5 10 15 Gln Val Leu Glu Gly Leu Asp Ala Val Arg Lys Arg Pro Gly Met Tyr 20 25 30 Ile Gly Ser Thr Asp Gly Ala Gly Leu His His Leu Val Trp Glu Ile 35 40 45 Val Asp Asn Ala Val Asp Glu Ala Leu Ser Gly Phe Gly Asp Arg Ile 50 55 60 Asp Val Thr Ile Asn Lys Asp Gly Ser Leu Thr Val Gln Asp His Gly 65 70 75 80 Arg Gly Met Pro Thr Gly Met His Ala Met Gly Ile Pro Thr Val Glu 85 90 95 Val Ile Phe Thr Ile Leu His Ala Gly Gly Lys Phe Gly Gln Gly Gly 100 105 110 Tyr Lys Thr Ser Gly Gly Leu His Gly Val Gly Ser Ser Val Val Asn 115 120 125 Ala Leu Ser Ser Trp Leu Glu Val Glu Ile Thr Arg Asp Gly Ala Val 130 135 140 Tyr Lys Gln Arg Phe Glu Asn Gly Gly Lys Pro Val Thr Thr Leu Lys 145 150 155 160 Lys Ile Gly Thr Ala Leu Lys Ser Lys Thr Gly Thr Lys Val Thr Phe 165 170 175 Met Pro Asp Ala Thr Ile Phe Ser Thr Thr Asp Phe Lys Tyr Asn Thr 180 185 190 Ile Ser Glu Arg Leu Asn Glu Ser Ala Phe Leu Leu Lys Asn Val Thr 195 200 205 Leu Ser Leu Thr Asp Lys Arg Thr Asp Glu Ala Ile Glu Phe His Tyr 210 215 220 Glu Asn Gly Val Gln Asp Phe Val Ser Tyr Leu Asn Glu Asp Lys Glu 225 230 235 240 Ile Leu Thr Pro Val Leu Tyr Phe Glu Gly Glu Asp Asn Gly Phe Gln 245 250 255 Val Glu Val Ala Leu Gln Tyr Asn Asp Gly Phe Ser Asp Asn Ile Leu 260 265 270 Ser Phe Val Asn Asn Val Arg Thr Lys Asp Gly Gly Thr His Glu Thr 275 280 285 Gly Leu Lys Ser Ala Ile Thr Lys Val Met Asn Asp Tyr Ala Arg Lys 290 295 300 Thr Gly Leu Leu Lys Glu Lys Asp Lys Asn Leu Glu Gly Ser Asp Tyr 305 310 315 320 Arg Glu Gly Leu Ala Ala Val Leu Ser Ile Leu Val Pro Glu Glu His 325 330 335 Leu Gln Phe Glu Gly Gln Thr Lys Asp Lys Leu Gly Ser Pro Leu Ala 340 345 350 Arg Pro Val Val Asp Gly Ile Val Ala Asp Lys Leu Thr Phe Phe Leu 355 360 365 Met Glu Asn Gly Glu Leu Ala Ser Asn Leu Ile Arg Lys Ala Ile Lys 370 375 380 Ala Arg Asp Ala Arg Glu Ala Ala Arg Lys Ala Arg Asp Glu Ser Arg 385 390 395 400 Asn Gly Lys Lys Asn Lys Lys Asp Lys Gly Leu Leu Ser Gly Lys Leu 405 410 415 Thr Pro Ala Gln Ser Lys Asn Pro Ala Lys Asn Glu Leu Tyr Leu Val 420 425 430 Glu Gly Asp Ser Ala Gly Gly Ser Ala Lys Gln Gly Arg Asp Arg Lys 435 440 445 Phe Gln Ala Ile Leu Pro Leu Arg Gly Lys Val Ile Asn Thr Ala Lys 450 455 460 Ala Lys Met Ala Asp Ile Leu Lys Asn Glu Glu Ile Asn Thr Met Ile 465 470 475 480 Tyr Thr Ile Gly Ala Gly Val Gly Ala Asp Phe Ser Ile Glu Asp Ala 485 490 495 Asn Tyr Asp Lys Ile Ile Ile Met Thr Asp Ala Asp Thr Asp Gly Ala 500 505 510 His Ile Gln Thr Leu Leu Leu Thr Phe Phe Tyr Arg Tyr Met Arg Pro 515 520 525 Leu Val Glu Ala Gly His Val Tyr Ile Ala Leu Pro Pro Leu Tyr Lys 530 535 540 Met Ser Lys Gly Lys Gly Lys Lys Glu Glu Val Ala Tyr Ala Trp Thr 545 550 555 560 Asp Gly Glu Leu Glu Glu Leu Arg Lys Gln Phe Gly Lys Gly Ala Thr 565 570 575 Leu Gln Arg Tyr Lys Gly Leu Gly Glu Met Asn Ala Asp Gln Leu Trp 580 585 590 Glu Thr Thr Met Asn Pro Glu Thr Arg Thr Leu Ile Arg Val Thr Ile 595 600 605 Glu Asp Leu Ala Arg Ala Glu Arg Arg Val Asn Val Leu Met Gly Asp 610 615 620 Lys Val Glu Pro Arg Arg Lys Trp Ile Glu Asp Asn Val Lys Phe Thr 625 630 635 640 Leu Glu Glu Ala Thr Val Phe 645 <210> 69 <211> 405 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (405) <223> Streptococcus pneumoniae spr0788 gene <400> 69 atgatgacga caggttgctc tatgggagcc tatcatgcac tcaatttctt cctccagcat 60 ccagatgtct ttaccaaagt gattgctctc agtggtgttt acgacgcacg tttctttgtc 120 ggtgattact acaacgatga tgctatttac caaaactcgc cagtagatta tatttggaac 180 caaaacgacg gctggtttat tgaccgttac cgtcaggcag agattgtgct gtgtacgggg 240 cttggagcct gggaacaaga tggtttgcca tccttttaca agctcaaaga agcctttgac 300 aagaaacaaa ttccagcctg gtttgctgaa tggggacatg atgtcgccca tgactgggaa 360 tggtggcgta aacaaatgcc ttatttcctc ggtaatctct attta 405 <210> 70 <211> 135 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (135) <223> Conserved hypothetical protein <400> 70 Met Met Thr Thr Gly Cys Ser Met Gly Ala Tyr His Ala Leu Asn Phe 1 5 10 15 Phe Leu Gln His Pro Asp Val Phe Thr Lys Val Ile Ala Leu Ser Gly 20 25 30 Val Tyr Asp Ala Arg Phe Phe Val Gly Asp Tyr Tyr Asn Asp Asp Ala 35 40 45 Ile Tyr Gln Asn Ser Pro Val Asp Tyr Ile Trp Asn Gln Asn Asp Gly 50 55 60 Trp Phe Ile Asp Arg Tyr Arg Gln Ala Glu Ile Val Leu Cys Thr Gly 65 70 75 80 Leu Gly Ala Trp Glu Gln Asp Gly Leu Pro Ser Phe Tyr Lys Leu Lys 85 90 95 Glu Ala Phe Asp Lys Lys Gln Ile Pro Ala Trp Phe Ala Glu Trp Gly 100 105 110 His Asp Val Ala His Asp Trp Glu Trp Trp Arg Lys Gln Met Pro Tyr 115 120 125 Phe Leu Gly Asn Leu Tyr Leu 130 135 <210> 71 <211> 1005 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1005) <223> Streptococcus pneumoniae spr0796 gene <400> 71 atgaaacgta ttgctgtttt gactagtggc ggagacgcac ctggtatgaa cgctgccatc 60 cgtgcagttg ttcgtcaagc aatttcagaa ggaatggaag tgtttggtat ctatgacgga 120 tatgctggta tggttgccgg tgaaattcat cccctagatg cagcttcagt aggggacatc 180 atttctcgtg gtggtacttt tcttcactca gctcgttatc cagagttcgc tcaacttgaa 240 gggcaactta aagggattga gcaattgaaa aaacacggaa ttgaaggtgt agttgttatc 300 ggtggtgacg gatcttacca cggcgctatg cgtttgactg aacatggctt cccagctatt 360 ggtcttccag gtacaatcga taacgatatc gttggtactg actttacaat cggttttgac 420 acagcggtta ctactgccat ggacgctatc gataagattc gtgatacatc atcaagtcac 480 cgtcgtactt ttgtaatcga agttatggga cgtaacgctg gtgatatcgc tctttgggct 540 ggtattgcaa ctggtgctga tgaaatcatc atccctgaag caggcttcaa gatggaagat 600 atcgtagcaa gcatcaaagc tggttatgaa tgtggtaaaa aacacaatat catcgtctta 660 gctgaaggtg tgatgtcagc ggctgaattt ggtcaaaaac ttaaagaagc tggagatata 720 agcgaccttc gtgtaacaga acttggacat attcaacgtg gtggttctcc aactccgcgt 780 gaccgtgttt tggcgtcacg tatgggtgca catgctgtta aacttcttaa agaaggtatc 840 ggtggtgttg cggttggtat tcgtaacgaa aaaatggttg aaaatccaat tcttggtact 900 gcagaagaag gggcattgtt tagccttact gcagaaggta agattgtggt taacaaccca 960 cacgaagccg atattgagct atctagcttg aataagagct tgtca 1005 <210> 72 <211> 335 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (335) <223> 6-phosphofructokinase I <400> 72 Met Lys Arg Ile Ala Val Leu Thr Ser Gly Gly Asp Ala Pro Gly Met 1 5 10 15 Asn Ala Ala Ile Arg Ala Val Val Arg Gln Ala Ile Ser Glu Gly Met 20 25 30 Glu Val Phe Gly Ile Tyr Asp Gly Tyr Ala Gly Met Val Ala Gly Glu 35 40 45 Ile His Pro Leu Asp Ala Ala Ser Val Gly Asp Ile Ile Ser Arg Gly 50 55 60 Gly Thr Phe Leu His Ser Ala Arg Tyr Pro Glu Phe Ala Gln Leu Glu 65 70 75 80 Gly Gln Leu Lys Gly Ile Glu Gln Leu Lys Lys His Gly Ile Glu Gly 85 90 95 Val Val Val Ile Gly Gly Asp Gly Ser Tyr His Gly Ala Met Arg Leu 100 105 110 Thr Glu His Gly Phe Pro Ala Ile Gly Leu Pro Gly Thr Ile Asp Asn 115 120 125 Asp Ile Val Gly Thr Asp Phe Thr Ile Gly Phe Asp Thr Ala Val Thr 130 135 140 Thr Ala Met Asp Ala Ile Asp Lys Ile Arg Asp Thr Ser Ser Ser His 145 150 155 160 Arg Arg Thr Phe Val Ile Glu Val Met Gly Arg Asn Ala Gly Asp Ile 165 170 175 Ala Leu Trp Ala Gly Ile Ala Thr Gly Ala Asp Glu Ile Ile Ile Pro 180 185 190 Glu Ala Gly Phe Lys Met Glu Asp Ile Val Ala Ser Ile Lys Ala Gly 195 200 205 Tyr Glu Cys Gly Lys Lys His Asn Ile Ile Val Leu Ala Glu Gly Val 210 215 220 Met Ser Ala Ala Glu Phe Gly Gln Lys Leu Lys Glu Ala Gly Asp Ile 225 230 235 240 Ser Asp Leu Arg Val Thr Glu Leu Gly His Ile Gln Arg Gly Gly Ser 245 250 255 Pro Thr Pro Arg Asp Arg Val Leu Ala Ser Arg Met Gly Ala His Ala 260 265 270 Val Lys Leu Leu Lys Glu Gly Ile Gly Gly Val Ala Val Gly Ile Arg 275 280 285 Asn Glu Lys Met Val Glu Asn Pro Ile Leu Gly Thr Ala Glu Glu Gly 290 295 300 Ala Leu Phe Ser Leu Thr Ala Glu Gly Lys Ile Val Val Asn Asn Pro 305 310 315 320 His Glu Ala Asp Ile Glu Leu Ser Ser Leu Asn Lys Ser Leu Ser 325 330 335 <210> 73 <211> 1503 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1503) <223> Streptococcus pneumoniae spr0797 gene <400> 73 atgaacaaac gtgtaaaaat cgttgcaact ttgggtcctg cggtagaaat ccgtggtggt 60 aaaaaattcg gtgaggacgg atactggggt gaaaaacttg atgttgaagc ttcagctaaa 120 aacattgcta aattgattga agctggtgct aacacattcc gattcaactt ctcacacggc 180 gaccaccaag aacaaggtga gcgtatggca actgttaaac ttgcggaaaa aattgcaggt 240 aaaaaagttg gtttccttct tgatacaaaa ggacctgaaa tccgtacaga attgttcgaa 300 ggtgaagcta aagaatattc atacaaaact ggtgaaaaaa ttcgtgttgc aactaaacaa 360 ggaatcaaat caactcgtga agtgattgcg ttgaacgttg ctggtgctct tgatatctat 420 gatgatgttg aagttggtcg tcaagttttg gttgacgatg gtaaacttgg tcttcgtgtg 480 gttgctaaag atgatgcaac tcgtgaattt gaagttgaag ttgaaaacga tggtatcatc 540 gctaaacaaa aaggtgtgaa catccctaac actaaaattc ctttcccagc tcttgctgaa 600 cgcgataacg acgatatccg tttcggtctt gaacaaggta tcaacttcat cgcaatttca 660 ttcgtacgta ctgcaaaaga tgtgaacgaa gttcgtgcaa tctgtgaaga aactggaaac 720 ggacatgttc aattgttcgc taaaatcgaa aaccaacaag gtatcgataa cttagacgaa 780 atcatcgaag cagctgatgg tattatgatt gctcgtggtg atatgggtat cgaagtaccg 840 ttcgaaatgg ttccagttta tcaaaaaatg attatcaaga aagtcaatgc tgcaggtaaa 900 gttgttatca ctgcaacaaa catgcttgaa acaatgactg aaaaaccacg tgcaactcgt 960 tcagaagtat cagacgtatt caacgctgtt atcgacggaa ctgacgctac aatgctgtca 1020 ggcgagtctg caaacggtaa atacccactc gagtcagtaa ctacaatggc tacaatcgac 1080 aagaacgctc aagctcttct taatgaatac ggacgtcttg attcagattc atttgagcgt 1140 aactctaaga cagaagtaat ggcttctgct gttaaagatg ctactagctc aatggatatc 1200 aaattggttg taactcttac taagacaggt catactgcac gtttgatttc taaataccgt 1260 ccaaatgctg acatcttagc attgacattt gacgaattga cagaacgtgg cttgatgttg 1320 aactggggtg ttatcccaat gttgacagat gctccatctt caactgacga tatgttcgaa 1380 atcgctgaac gtaaagcggt agaagcaggt ctcgttgagt caggcgatga tatcgttatc 1440 gttgctggtg tgccagtagg agaagctgtt cgcacaaaca caatgcgtat ccgcacagta 1500 cgt 1503 <210> 74 <211> 501 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (501) <223> Pyruvate kinase I <400> 74 Met Asn Lys Arg Val Lys Ile Val Ala Thr Leu Gly Pro Ala Val Glu 1 5 10 15 Ile Arg Gly Gly Lys Lys Phe Gly Glu Asp Gly Tyr Trp Gly Glu Lys 20 25 30 Leu Asp Val Glu Ala Ser Ala Lys Asn Ile Ala Lys Leu Ile Glu Ala 35 40 45 Gly Ala Asn Thr Phe Arg Phe Asn Phe Ser His Gly Asp His Gln Glu 50 55 60 Gln Gly Glu Arg Met Ala Thr Val Lys Leu Ala Glu Lys Ile Ala Gly 65 70 75 80 Lys Lys Val Gly Phe Leu Leu Asp Thr Lys Gly Pro Glu Ile Arg Thr 85 90 95 Glu Leu Phe Glu Gly Glu Ala Lys Glu Tyr Ser Tyr Lys Thr Gly Glu 100 105 110 Lys Ile Arg Val Ala Thr Lys Gln Gly Ile Lys Ser Thr Arg Glu Val 115 120 125 Ile Ala Leu Asn Val Ala Gly Ala Leu Asp Ile Tyr Asp Asp Val Glu 130 135 140 Val Gly Arg Gln Val Leu Val Asp Asp Gly Lys Leu Gly Leu Arg Val 145 150 155 160 Val Ala Lys Asp Asp Ala Thr Arg Glu Phe Glu Val Glu Val Glu Asn 165 170 175 Asp Gly Ile Ile Ala Lys Gln Lys Gly Val Asn Ile Pro Asn Thr Lys 180 185 190 Ile Pro Phe Pro Ala Leu Ala Glu Arg Asp Asn Asp Asp Ile Arg Phe 195 200 205 Gly Leu Glu Gln Gly Ile Asn Phe Ile Ala Ile Ser Phe Val Arg Thr 210 215 220 Ala Lys Asp Val Asn Glu Val Arg Ala Ile Cys Glu Glu Thr Gly Asn 225 230 235 240 Gly His Val Gln Leu Phe Ala Lys Ile Glu Asn Gln Gln Gly Ile Asp 245 250 255 Asn Leu Asp Glu Ile Ile Glu Ala Ala Asp Gly Ile Met Ile Ala Arg 260 265 270 Gly Asp Met Gly Ile Glu Val Pro Phe Glu Met Val Pro Val Tyr Gln 275 280 285 Lys Met Ile Ile Lys Lys Val Asn Ala Ala Gly Lys Val Val Ile Thr 290 295 300 Ala Thr Asn Met Leu Glu Thr Met Thr Glu Lys Pro Arg Ala Thr Arg 305 310 315 320 Ser Glu Val Ser Asp Val Phe Asn Ala Val Ile Asp Gly Thr Asp Ala 325 330 335 Thr Met Leu Ser Gly Glu Ser Ala Asn Gly Lys Tyr Pro Leu Glu Ser 340 345 350 Val Thr Thr Met Ala Thr Ile Asp Lys Asn Ala Gln Ala Leu Leu Asn 355 360 365 Glu Tyr Gly Arg Leu Asp Ser Asp Ser Phe Glu Arg Asn Ser Lys Thr 370 375 380 Glu Val Met Ala Ser Ala Val Lys Asp Ala Thr Ser Ser Met Asp Ile 385 390 395 400 Lys Leu Val Val Thr Leu Thr Lys Thr Gly His Thr Ala Arg Leu Ile 405 410 415 Ser Lys Tyr Arg Pro Asn Ala Asp Ile Leu Ala Leu Thr Phe Asp Glu 420 425 430 Leu Thr Glu Arg Gly Leu Met Leu Asn Trp Gly Val Ile Pro Met Leu 435 440 445 Thr Asp Ala Pro Ser Ser Thr Asp Asp Met Phe Glu Ile Ala Glu Arg 450 455 460 Lys Ala Val Glu Ala Gly Leu Val Glu Ser Gly Asp Asp Ile Val Ile 465 470 475 480 Val Ala Gly Val Pro Val Gly Glu Ala Val Arg Thr Asn Thr Met Arg 485 490 495 Ile Arg Thr Val Arg 500 <210> 75 <211> 741 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (741) <223> Streptococcus pneumoniae spr0845 gene <400> 75 gtgaaaatgg cgaatcccaa gtataaacgt attttaatca agttatcagg tgaagccctt 60 gccggtgaac gtggcgtagg gattgatatc caaacagttc aaacaatcgc aaaagagatt 120 caagaagttc atagcttagg tatcgaaatt gcccttgtta ttggtggagg aaatctttgg 180 cgtggagaac ctgcagcaga agcaggtatg gaccgtgttc aggcagatta cactggaatg 240 cttgggactg ttatgaatgc tcttgtgatg gcagattcat tgcaacaagt tggggttgat 300 acgcgtgtac aaacagctat tgctatgcaa caagtggcag agccttatgt ccgtggacgt 360 gcccttcgtc accttgaaaa aggccgtatc gttatctttg gtgctggaat tggttcacca 420 tacttctcga cagatacaac agcggccctt cgtgcagctg aaatcgaagc agatgccatc 480 ctcatggcta aaaatggcgt cgatggtgtg tacaatgccg atcctaagaa ggacaagaca 540 gccgttaagt ttgaagaatt gacccaccgt gatgttatca acaaaggtct tcgtatcatg 600 gactcaacag cctcaaccct ctcaatggac aacgacattg acttggttgt cttcaacatg 660 aaccaatcag gcaacatcaa acgtgtcgta tttggtgaaa atatcggaac aacagtttca 720 aataatatcg aagaaaagga a 741 <210> 76 <211> 396 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (396) <223> UMP kinase <400> 76 Met Ser Glu Arg Lys Leu Phe Thr Ser Glu Ser Val Ser Glu Gly His 1 5 10 15 Pro Asp Lys Ile Ala Asp Gln Ile Ser Asp Ala Ile Leu Asp Ala Ile 20 25 30 Leu Ala Lys Asp Pro Glu Ala His Val Ala Ala Glu Thr Ala Val Tyr 35 40 45 Thr Gly Ser Val His Val Phe Gly Glu Ile Ser Thr Asn Ala Tyr Val 50 55 60 Asp Ile Asn Arg Val Val Arg Asp Thr Ile Ala Glu Ile Gly Tyr Thr 65 70 75 80 Asn Thr Glu Tyr Gly Phe Ser Ala Glu Thr Val Gly Val His Pro Ser 85 90 95 Leu Val Glu Gln Ser Pro Asp Ile Ala Gln Gly Val Asn Glu Ala Leu 100 105 110 Glu Val Arg Gly Asn Ala Asp Gln Asp Pro Leu Asp Leu Ile Gly Ala 115 120 125 Gly Asp Gln Gly Leu Met Phe Gly Phe Ala Val Asp Glu Thr Glu Glu 130 135 140 Leu Met Pro Leu Pro Ile Ala Leu Ser His Lys Leu Val Arg Arg Leu 145 150 155 160 Ala Glu Leu Arg Lys Ser Gly Glu Ile Ser Tyr Leu Arg Pro Asp Ala 165 170 175 Lys Ser Gln Val Thr Val Glu Tyr Asp Glu Asn Asp Arg Pro Val Arg 180 185 190 Val Asp Thr Val Val Ile Ser Thr Gln His Asp Pro Glu Ala Thr Asn 195 200 205 Glu Gln Ile His Gln Asp Val Ile Asp Lys Val Ile Lys Glu Val Ile 210 215 220 Pro Ser Ser Tyr Leu Asp Asp Lys Thr Lys Phe Phe Ile Asn Pro Thr 225 230 235 240 Gly Arg Phe Val Ile Gly Gly Pro Gln Gly Asp Ser Gly Leu Thr Gly 245 250 255 Arg Lys Ile Ile Val Asp Thr Tyr Gly Gly Tyr Ser Arg His Gly Gly 260 265 270 Gly Ala Phe Ser Gly Lys Asp Ala Thr Lys Val Asp Arg Ser Ala Ser 275 280 285 Tyr Ala Ala Arg Tyr Ile Ala Lys Asn Ile Val Ala Ala Gly Leu Ala 290 295 300 Lys Lys Ala Glu Val Gln Leu Ala Tyr Ala Ile Gly Val Ala Gln Pro 305 310 315 320 Val Ser Val Arg Ile Asp Thr Phe Gly Thr Gly Thr Val Ala Glu Ser 325 330 335 Gln Leu Glu Lys Ala Ala Arg Gln Ile Phe Asp Leu Arg Pro Ala Gly 340 345 350 Ile Ile Gln Met Leu Asp Leu Lys Arg Pro Ile Tyr Arg Gln Thr Ser 355 360 365 Ala Tyr Gly His Met Gly Arg Thr Asp Ile Asp Leu Pro Trp Glu Arg 370 375 380 Leu Asp Lys Val Asp Ala Leu Lys Glu Ala Val Lys 385 390 395 <210> 77 <211> 648 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (648) <223> Streptococcus pneumoniae spr0856 gene <400> 77 atggaagcaa ttatcgagaa aatcaaagag tataaaatca tcgtcatctg tactggtctg 60 ggcttgcttg taggaggatt tttcctgcta aaaccagctc cacaaacacc tgtcaaagag 120 acgaatttgc aggctgaagt cgcagctgtt tccaaggatt tggtatccga aaaggaagtg 180 aacaaggaag aaaaggaaga accccttgaa caagatctaa tcacagtaga tgtcaaaggt 240 gctgtcaaat cgccagggat ttatgacttg cctgtaggta gtcgagtcaa tgatgctgtt 300 cagaaggctg gtggcttgac agagcaagca gacagcaagt cgctcaatct agctcagaaa 360 gttagtgatg aggctctggt ttacgttcct actaagggag aagaagcagt tagccaacag 420 actggtttgg ggacagcttc ttcaataagc aaggaaaaga aggtcaatct caacaaggcc 480 agtctggaag aactcaagca ggtcaaggga ctgggaggaa aacgagctca ggacattatc 540 gaccatcgtg aggcaaatgg caagttcaag tcagtagacg agctcaagaa ggtctctggc 600 attggtggca aaacaataga aaagcttaaa gactatgtta cagtggat 648 <210> 78 <211> 216 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (216) <223> Competence protein CelA <400> 78 Met Glu Ala Ile Ile Glu Lys Ile Lys Glu Tyr Lys Ile Ile Val Ile 1 5 10 15 Cys Thr Gly Leu Gly Leu Leu Val Gly Gly Phe Phe Leu Leu Lys Pro 20 25 30 Ala Pro Gln Thr Pro Val Lys Glu Thr Asn Leu Gln Ala Glu Val Ala 35 40 45 Ala Val Ser Lys Asp Leu Val Ser Glu Lys Glu Val Asn Lys Glu Glu 50 55 60 Lys Glu Glu Pro Leu Glu Gln Asp Leu Ile Thr Val Asp Val Lys Gly 65 70 75 80 Ala Val Lys Ser Pro Gly Ile Tyr Asp Leu Pro Val Gly Ser Arg Val 85 90 95 Asn Asp Ala Val Gln Lys Ala Gly Gly Leu Thr Glu Gln Ala Asp Ser 100 105 110 Lys Ser Leu Asn Leu Ala Gln Lys Val Ser Asp Glu Ala Leu Val Tyr 115 120 125 Val Pro Thr Lys Gly Glu Glu Ala Val Ser Gln Gln Thr Gly Leu Gly 130 135 140 Thr Ala Ser Ser Ile Ser Lys Glu Lys Lys Val Asn Leu Asn Lys Ala 145 150 155 160 Ser Leu Glu Glu Leu Lys Gln Val Lys Gly Leu Gly Lys Arg Ala 165 170 175 Gln Asp Ile Ile Asp His Arg Glu Ala Asn Gly Lys Phe Lys Ser Val 180 185 190 Asp Glu Leu Lys Lys Val Ser Gly Ile Gly Gly Lys Thr Ile Glu Lys 195 200 205 Leu Lys Asp Tyr Val Thr Val Asp 210 215 <210> 79 <211> 585 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (585) <223> Streptococcus pneumoniae spr0861 gene <400> 79 gtgcgggctt gtatatttac gagtccgcta ttgtttttct ctaataaaac aaaagaggtg 60 aaaaccatag caaagcaaga cttattcatc aatgatgaga ttcgtgtacg tgaagttcgc 120 ttgattggtc ttgagggaga acagctaggc atcaagccac tcagtgaagc gcaagctttg 180 gctgataacg ctaatgttga cctagtattg attcaacccc aagccaaacc gcctgttgca 240 aaaattatgg actacggtaa gttcaaattt gagtaccaga agaagcaaaa agaacaacgt 300 aaaaaacaaa gcgttgttac tgtgaaagaa gttcgtctaa gtccaactat tgacaagggt 360 gactttgata caaaacttcg taatgcacgt aagttccttg aaaaaggaaa taaagttaag 420 gtatctattc gctttaaggg tcgtatgatt acccataaag agattggtgc aaaagtttta 480 gccgagtttg ctgaagcaac tcaagatatt gccatcatcg aacaacgtgc taaaatggat 540 ggacgtcaaa tgttcatgca attggcgcca gcgactgaca aaaaa 585 <210> 80 <211> 195 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (195) <223> Translation initiation factor IF-3 <400> 80 Met Arg Ala Cys Ile Phe Thr Ser Pro Leu Leu Phe Phe Ser Asn Lys 1 5 10 15 Thr Lys Glu Val Lys Thr Ile Ala Lys Gln Asp Leu Phe Ile Asn Asp 20 25 30 Glu Ile Arg Val Arg Glu Val Arg Leu Ile Gly Leu Glu Gly Glu Gln 35 40 45 Leu Gly Ile Lys Pro Leu Ser Glu Ala Gln Ala Leu Ala Asp Asn Ala 50 55 60 Asn Val Asp Leu Val Leu Ile Gln Pro Gln Ala Lys Pro Pro Val Ala 65 70 75 80 Lys Ile Met Asp Tyr Gly Lys Phe Lys Phe Glu Tyr Gln Lys Lys Gln 85 90 95 Lys Glu Gln Arg Lys Lys Gln Ser Val Val Thr Val Lys Glu Val Arg 100 105 110 Leu Ser Pro Thr Ile Asp Lys Gly Asp Phe Asp Thr Lys Leu Arg Asn 115 120 125 Ala Arg Lys Phe Leu Glu Lys Gly Asn Lys Val Lys Val Ser Ile Arg 130 135 140 Phe Lys Gly Arg Met Ile Thr His Lys Glu Ile Gly Ala Lys Val Leu 145 150 155 160 Ala Glu Phe Ala Glu Ala Thr Gln Asp Ile Ala Ile Ile Glu Gln Arg 165 170 175 Ala Lys Met Asp Gly Arg Gln Met Phe Met Gln Leu Ala Pro Ala Thr 180 185 190 Asp Lys Lys 195 <210> 81 <211> 951 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (951) <223> Streptococcus pneumoniae spr0881 gene <400> 81 atgtttgttg cgagagatgc taggggagaa ttggtaaatg tgttagagga taaacttgag 60 aagcaagcat acacctgccc agcttgtgga ggccagctcc atttgcgtca aggaccaagt 120 gtacggacgc attttgccca taaatcttta aaagactgtg attttttctt tgaaaatgaa 180 agtccagaac acctggccaa taaggaatcc ctctatcact ggttgaaaaa agagacaaag 240 gttcaattag agtacccgct ttcagaactt aaacagattg cggatgtatt tgtaaatggc 300 aatctagctc tagaagttca gtgtagtccc ttgcctcaga aagtccttaa agagcgaagt 360 gagggctatc gtagtcaggg ttaccaagta ctgtggttgc tgggtcaaaa actgtggctc 420 aaggagcgtt tgactcgtct acagcaaggt tttctttatt tcagtcaaaa catgggcttt 480 tatgtttggg aattagacaa gggaaaacaa gttttaagac tcaaatacct gatttaccag 540 gatctccgcg gtaaactcca ttatcaaatc aaggaatttt cctatggcca aggtagttta 600 ttggaaatat tgcgtcttcc ctataagaaa caaaaaatat ctcattttac agtttctgag 660 gacaaggaca tctgtcgcta tatccggcaa caactttatt atcaaaatct cttttggatg 720 aaagaacaag cagaagccta tcaaaaggga gaaaatatcc tgacttatgg actgaaagaa 780 tggtatccac aaattcgacc aatagtgggc aaatttttcc agattgaaca agacttgact 840 agctattatc agcactttta tacctattac caaaaaaatc ctcaaaatga ttggcaaaag 900 ctttatccac cagcctttta tcagcaatat ttcttgaaaa atatggtaga a 951 <210> 82 <211> 317 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (317) <223> Competence protein CoiA <400> 82 Met Phe Val Ala Arg Asp Ala Arg Gly Glu Leu Val Asn Val Leu Glu 1 5 10 15 Asp Lys Leu Glu Lys Gln Ala Tyr Thr Cys Pro Ala Cys Gly Gly Gln 20 25 30 Leu His Leu Arg Gln Gly Pro Ser Val Arg Thr His Phe Ala His Lys 35 40 45 Ser Leu Lys Asp Cys Asp Phe Phe Phe Glu Asn Glu Ser Pro Glu His 50 55 60 Leu Ala Asn Lys Glu Ser Leu Tyr His Trp Leu Lys Lys Glu Thr Lys 65 70 75 80 Val Gln Leu Glu Tyr Pro Leu Ser Glu Leu Lys Gln Ile Ala Asp Val 85 90 95 Phe Val Asn Gly Asn Leu Ala Leu Glu Val Gln Cys Ser Pro Leu Pro 100 105 110 Gln Lys Val Leu Lys Glu Arg Ser Glu Gly Tyr Arg Ser Gln Gly Tyr 115 120 125 Gln Val Leu Trp Leu Leu Gly Gln Lys Leu Trp Leu Lys Glu Arg Leu 130 135 140 Thr Arg Leu Gln Gln Gly Phe Leu Tyr Phe Ser Gln Asn Met Gly Phe 145 150 155 160 Tyr Val Trp Glu Leu Asp Lys Gly Lys Gln Val Leu Arg Leu Lys Tyr 165 170 175 Leu Ile Tyr Gln Asp Leu Arg Gly Lys Leu His Tyr Gln Ile Lys Glu 180 185 190 Phe Ser Tyr Gly Gln Gly Ser Leu Leu Glu Ile Leu Arg Leu Pro Tyr 195 200 205 Lys Lys Gln Lys Ile Ser His Phe Thr Val Ser Glu Asp Lys Asp Ile 210 215 220 Cys Arg Tyr Ile Arg Gln Gln Leu Tyr Tyr Gln Asn Leu Phe Trp Met 225 230 235 240 Lys Glu Gln Ala Glu Ala Tyr Gln Lys Gly Glu Asn Ile Leu Thr Tyr 245 250 255 Gly Leu Lys Glu Trp Tyr Pro Gln Ile Arg Pro Ile Val Gly Lys Phe 260 265 270 Phe Gln Ile Glu Gln Asp Leu Thr Ser Tyr Tyr Gln His Phe Tyr Thr 275 280 285 Tyr Tyr Gln Lys Asn Pro Gln Asn Asp Trp Gln Lys Leu Tyr Pro Pro 290 295 300 Ala Phe Tyr Gln Gln Tyr Phe Leu Lys Asn Met Val Glu 305 310 315 <210> 83 <211> 1800 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1800) <223> Streptococcus pneumoniae spr0882 gene <400> 83 atggtattac aaagaaatga aataaatgaa aaagatacat gggatctatc aacgatctac 60 ccaactgacc aggcttggga agaagcctta aaagatttaa cagaacaatt ggagacagta 120 gcccagtatg aaggccatct cttggatagt gcggataacc tactagaaat cactgaattt 180 tctcttgaaa tggaacgcca gatagagaag ctttacgttt atgctcatat gaagaatgac 240 caggatacac gtgaagctaa gtatcaagag tactatgcca aggccatgac actctacagc 300 cagttagacc aagccttttc attctatgag cctgaattta tggagattag cgaaaagcag 360 tatgctgact ttttagaagc tcaaccaaag ctgcaggttt atcaacacta ttttgacaag 420 ctcttgcaag gcaaggatca cgttctttca caacgtgaag aagaattatt ggctggagct 480 ggagaaatct ttggttcagc aagtgaaacc ttcgctatct tggacaatgc ggatattgtg 540 ttcccttatg tcctagacga tgatggtaaa gaagttcagc tatctcatgg gacttacaca 600 cgtttgatgg agtctaaaaa acgtgaggtt cgccgtggtg cctatcaagc tctttatgcg 660 acttacgaac aattccaaca cacctatgcc aaaaccttgc aaaccaatgt taaggtgcaa 720 aattaccgtg ctaaagttcg taactacaag agtgctcgtc atgcagccct cgcagcgaat 780 tttgttccag aaagtgttta tgacaatttg gtagcagcag ttcgcaagca tttgccactc 840 ttacatcgct atcttgagct tcgttcaaaa atcttgggga tttcagatct caagatgtac 900 gatgtctaca caccgctttc atctgttgaa tacagtttta cctaccaaga agccttgaaa 960 aaagcagaag atgctttggc agtcttgggt gaggattact tgagccgtgt taaacgtgcc 1020 ttcagcgagc gttggattga tgtttacgaa aatcaaggca agcgttcagg tgcctactct 1080 ggtggttctt acgataccaa tgcctttatg cttctcaact ggcaggacaa tctggacaat 1140 ctctttactc ttgttcatga aacaggtcac agtatgcatt caagctatac tcgtgaaact 1200 cagccttatg tttacggaga ttactctatc tttttggctg agattgcctc aactaccaat 1260 gaaaatatct tgacggagaa attattggaa gaagtggaag acgacgcaac acgctttgct 1320 attctcaata acttcctaga tggtttccgt ggaacagttt tccgccaaac tcaatttgct 1380 gagtttgaac acgccattca ccaagcagat caaaatgggg aggtcttgac aagcgatttc 1440 ctaaataaac tctacgcaga cttgaaccaa gagtattatg gtttgagtaa ggaagacaat 1500 cctgaaatcc aatacgagtg ggctcgcatt ccacacttct actataacta ctatgtatat 1560 caatattcaa ctggctttgc ggccgcctca gccttggctg aaaaaattgt ccatggtagt 1620 caagaagacc gtgaccgcta tatcgactac ctcaaggcag gtaagtcgga ctatccactt 1680 aatgtcatga gaaaagctgg tgttgatatg gagaaggaag actacctcaa cgatgccttt 1740 gcagtctttg aacgccgttt aaatgagttt gaagcccttg ttgaaaaatt aggattggca 1800 1800 <210> 84 <211> 600 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (600) <223> Group B oligopeptidase <400> 84 Met Val Leu Gln Arg Asn Glu Ile Asn Glu Lys Asp Thr Trp Asp Leu 1 5 10 15 Ser Thr Ile Tyr Pro Thr Asp Gln Ala Trp Glu Glu Ala Leu Lys Asp 20 25 30 Leu Thr Glu Gln Leu Glu Thr Val Ala Gln Tyr Glu Gly His Leu Leu 35 40 45 Asp Ser Ala Asp Asn Leu Leu Glu Ile Thr Glu Phe Ser Leu Glu Met 50 55 60 Glu Arg Gln Ile Glu Lys Leu Tyr Val Tyr Ala His Met Lys Asn Asp 65 70 75 80 Gln Asp Thr Arg Glu Ala Lys Tyr Gln Glu Tyr Tyr Ala Lys Ala Met 85 90 95 Thr Leu Tyr Ser Gln Leu Asp Gln Ala Phe Ser Phe Tyr Glu Pro Glu 100 105 110 Phe Met Glu Ile Ser Glu Lys Gln Tyr Ala Asp Phe Leu Glu Ala Gln 115 120 125 Pro Lys Leu Gln Val Tyr Gln His Tyr Phe Asp Lys Leu Leu Gln Gly 130 135 140 Lys Asp His Val Leu Ser Gln Arg Glu Glu Glu Leu Leu Ala Gly Ala 145 150 155 160 Gly Glu Ile Phe Gly Ser Ala Ser Glu Thr Phe Ala Ile Leu Asp Asn 165 170 175 Ala Asp Ile Val Phe Pro Tyr Val Leu Asp Asp Asp Gly Lys Glu Val 180 185 190 Gln Leu Ser His Gly Thr Tyr Thr Arg Leu Met Glu Ser Lys Lys Arg 195 200 205 Glu Val Arg Arg Gly Ala Tyr Gln Ala Leu Tyr Ala Thr Tyr Glu Gln 210 215 220 Phe Gln His Thr Tyr Ala Lys Thr Leu Gln Thr Asn Val Lys Val Gln 225 230 235 240 Asn Tyr Arg Ala Lys Val Arg Asn Tyr Lys Ser Ala Arg His Ala Ala 245 250 255 Leu Ala Ala Asn Phe Val Pro Glu Ser Val Tyr Asp Asn Leu Val Ala 260 265 270 Ala Val Arg Lys His Leu Pro Leu Leu His Arg Tyr Leu Glu Leu Arg 275 280 285 Ser Lys Ile Leu Gly Ile Ser Asp Leu Lys Met Tyr Asp Val Tyr Thr 290 295 300 Pro Leu Ser Ser Val Glu Tyr Ser Phe Thr Tyr Gln Glu Ala Leu Lys 305 310 315 320 Lys Ala Glu Asp Ala Leu Ala Val Leu Gly Glu Asp Tyr Leu Ser Arg 325 330 335 Val Lys Arg Ala Phe Ser Glu Arg Trp Ile Asp Val Tyr Glu Asn Gln 340 345 350 Gly Lys Arg Ser Gly Ala Tyr Ser Gly Gly Ser Tyr Asp Thr Asn Ala 355 360 365 Phe Met Leu Leu Asn Trp Gln Asp Asn Leu Asp Asn Leu Phe Thr Leu 370 375 380 Val His Glu Thr Gly His Ser Met His Ser Ser Tyr Thr Arg Glu Thr 385 390 395 400 Gln Pro Tyr Val Tyr Gly Asp Tyr Ser Ile Phe Leu Ala Glu Ile Ala 405 410 415 Ser Thr Thr Asn Glu Asn Ile Leu Thr Glu Lys Leu Leu Glu Glu Val 420 425 430 Glu Asp Asp Ala Thr Arg Phe Ala Ile Leu Asn Asn Phe Leu Asp Gly 435 440 445 Phe Arg Gly Thr Val Phe Arg Gln Thr Gln Phe Ala Glu Phe Glu His 450 455 460 Ala Ile His Gln Ala Asp Gln Asn Gly Glu Val Leu Thr Ser Asp Phe 465 470 475 480 Leu Asn Lys Leu Tyr Ala Asp Leu Asn Gln Glu Tyr Tyr Gly Leu Ser 485 490 495 Lys Glu Asp Asn Pro Glu Ile Gln Tyr Glu Trp Ala Arg Ile Pro His 500 505 510 Phe Tyr Tyr Asn Tyr Tyr Val Tyr Gln Tyr Ser Thr Gly Phe Ala Ala 515 520 525 Ala Ser Ala Leu Ala Glu Lys Ile Val His Gly Ser Gln Glu Asp Arg 530 535 540 Asp Arg Tyr Ile Asp Tyr Leu Lys Ala Gly Lys Ser Asp Tyr Pro Leu 545 550 555 560 Asn Val Met Arg Lys Ala Gly Val Asp Met Glu Lys Glu Asp Tyr Leu 565 570 575 Asn Asp Ala Phe Ala Val Phe Glu Arg Arg Leu Asn Glu Phe Glu Ala 580 585 590 Leu Val Glu Lys Leu Gly Leu Ala 595 600 <210> 85 <211> 933 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (933) <223> Streptococcus pneumoniae spr0906 gene <400> 85 atggttaagg agttgttcat gaagaaacaa aatttatttt tagtcctgtt aagtgtcttt 60 cttttatgct tgggggcttg tggtcaaaag gaaagtcaga caggaaaggg gatgaaaatt 120 gtgaccagtt tttatcctat ctacgctatg gttaaggaag tatctggtga cttgaatgat 180 gttcggatga ttcagtcaag tagtggtatt cactcctttg aaccttcggc aaatgatatc 240 gcagccatct atgatgcaga tgtctttgtt taccattctc atacactcga atcttgggca 300 ggaagtctgg atccaaatct aaaaaaatcc aaagtgaagg tcttagaggc ttctgaggga 360 atgaccttgg aacgtgtccc tggactagag gatgtggaag caggggatgg agttgatgaa 420 aaaacgctct atgaccctca cacatggcta gatcctgaaa aagctggaga agaagcccaa 480 attatcgctg ataaactttc agaggtggat agtgagcata aagagactta tcaaaaaaat 540 gcgcaagcct ttatcaaaaa agctcaggaa ttgactaaga aattccaacc aaaatttgaa 600 aaagcgactc agaaaacatt tgtaacacaa catacagcct tttcttatct agcgaagaga 660 tttgggctta atcaacttgg tattgcaggt atctctcctg aacaagaacc aagtccacga 720 caactaacag aaattcagga atttgttaag acctataagg ttaaaacgat ttttacagaa 780 agtaacgctt cttcaaaagt agctgaaact cttgtcaaat caacaggtgt gggtcttaaa 840 actctgaatc ctttagagtc agacccacaa aatgacaaga cctatttaga aaatcttgaa 900 gaaaatatga gtattctagc agaagaatta aag 933 <210> 86 <211> 311 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (311) <223> Lipoprotein <400> 86 Met Val Lys Glu Leu Phe Met Lys Lys Gln Asn Leu Phe Leu Val Leu 1 5 10 15 Leu Ser Val Phe Leu Leu Cys Leu Gly Ala Cys Gly Gln Lys Glu Ser 20 25 30 Gln Thr Gly Lys Gly Met Lys Ile Val Thr Ser Phe Tyr Pro Ile Tyr 35 40 45 Ala Met Val Lys Glu Val Ser Gly Asp Leu Asn Asp Val Arg Met Ile 50 55 60 Gln Ser Ser Ser Gly Ile His Ser Phe Glu Pro Ser Ala Asn Asp Ile 65 70 75 80 Ala Ala Ile Tyr Asp Ala Asp Val Phe Val Tyr His Ser His Thr Leu 85 90 95 Glu Ser Trp Ala Gly Ser Leu Asp Pro Asn Leu Lys Lys Ser Lys Val 100 105 110 Lys Val Leu Glu Ala Ser Glu Gly Met Thr Leu Glu Arg Val Pro Gly 115 120 125 Leu Glu Asp Val Glu Ala Gly Asp Gly Val Asp Glu Lys Thr Leu Tyr 130 135 140 Asp Pro His Thr Trp Leu Asp Pro Glu Lys Ala Gly Glu Glu Ala Gln 145 150 155 160 Ile Ile Ala Asp Lys Leu Ser Glu Val Asp Ser Glu His Lys Glu Thr 165 170 175 Tyr Gln Lys Asn Ala Gln Ala Phe Ile Lys Lys Ala Gln Glu Leu Thr 180 185 190 Lys Lys Phe Gln Pro Lys Phe Glu Lys Ala Thr Gln Lys Thr Phe Val 195 200 205 Thr Gln His Thr Ala Phe Ser Tyr Leu Ala Lys Arg Phe Gly Leu Asn 210 215 220 Gln Leu Gly Ile Ala Gly Ile Ser Pro Glu Gln Glu Pro Ser Pro Arg 225 230 235 240 Gln Leu Thr Glu Ile Gln Glu Phe Val Lys Thr Tyr Lys Val Lys Thr 245 250 255 Ile Phe Thr Glu Ser Asn Ala Ser Ser Lys Val Ala Glu Thr Leu Val 260 265 270 Lys Ser Thr Gly Val Gly Leu Lys Thr Leu Asn Pro Leu Glu Ser Asp 275 280 285 Pro Gln Asn Asp Lys Thr Tyr Leu Glu Asn Leu Glu Glu Asn Met Ser 290 295 300 Ile Leu Ala Glu Glu Leu Lys 305 310 <210> 87 <211> 1104 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1104) <223> Streptococcus pneumoniae spr0965 gene <400> 87 atggaatcaa aaatttcaat tttagataga ctatattcat ggatttatat taagaaatta 60 aaaaaactag gtgcaattat agaagagaat gttgtgattt gctttggtgc aaagcttttt 120 ttcaatgaag aatgcttaat tcagaaggat acggtcatcg gtagatttgt actaatagaa 180 gcaaatagga taacaattgg gaataattgc ttatttttcc cacgaacttt aatttattcc 240 aaggaaacat ttagtttggg aacacgagga aaaattagta aagattgtat atttagggcg 300 aataaaataa atattggaag agaattttgg tgcaatgagg cagtaagaat tggagaaggc 360 ggttggaatc aaaaaagtgc aaatattaaa attggtgatt atcaattcat aggaccacga 420 gctcaaatta atgtaagtga ttctgtggaa ctcatgggat atggtggtct aggaatcgag 480 acgatgatct ttacgcatgg tgcgggacat ggccaatcag caactgatgg attttatgct 540 gaacaaaata aagttattat tcaaaaaaat gtctcaattt taacaagagc tattattcta 600 ccaggtgtta tagtcagtca aggcaccact gtagcggcaa atgctatcgt aaccaagagt 660 tttccaaagc actcgttaat aggaggagta ccggctaggt atattggtca gagtaataaa 720 gaaatttctg tgaaagagca aaaaaatatt attgttgaca tattaaaaga aggattagga 780 acagaaccag tgataaaaaa caatagcttt tgttttgaga aatttaatga aaatattact 840 tttcaatatg atttagaaaa aattgaatca acagacaata ttagtcaaag agatattatt 900 atattttatc aaggtacaaa taaatgtcat aaaaattata gcacctgtat tgatttgaag 960 tctaaaacaa tttcaggtag agcaagtaag gcttcagaat ttttaagaga taaatttaga 1020 agaaaaggaa taatcttaaa ttataaaaat tatagtccat tttcattgaa ttatgattat 1080 ttaataatta ataagattga agtt 1104 <210> 88 <211> 368 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (368) <223> Conserved hypothetical protein <400> 88 Met Glu Ser Lys Ile Ser Ile Leu Asp Arg Leu Tyr Ser Trp Ile Tyr 1 5 10 15 Ile Lys Lys Leu Lys Lys Leu Gly Ala Ile Ile Glu Glu Asn Val Val 20 25 30 Ile Cys Phe Gly Ala Lys Leu Phe Phe Asn Glu Glu Cys Leu Ile Gln 35 40 45 Lys Asp Thr Val Ile Gly Arg Phe Val Leu Ile Glu Ala Asn Arg Ile 50 55 60 Thr Ile Gly Asn Asn Cys Leu Phe Phe Pro Arg Thr Leu Ile Tyr Ser 65 70 75 80 Lys Glu Thr Phe Ser Leu Gly Thr Arg Gly Lys Ile Ser Lys Asp Cys 85 90 95 Ile Phe Arg Ala Asn Lys Ile Asn Ile Gly Arg Glu Phe Trp Cys Asn 100 105 110 Glu Ala Val Arg Ile Gly Glu Gly Gly Trp Asn Gln Lys Ser Ala Asn 115 120 125 Ile Lys Ile Gly Asp Tyr Gln Phe Ile Gly Pro Arg Ala Gln Ile Asn 130 135 140 Val Ser Asp Ser Val Glu Leu Met Gly Tyr Gly Gly Leu Gly Ile Glu 145 150 155 160 Thr Met Ile Phe Thr His Gly Ala Gly His Gly Gln Ser Ala Thr Asp 165 170 175 Gly Phe Tyr Ala Glu Gln Asn Lys Val Ile Ile Gln Lys Asn Val Ser 180 185 190 Ile Leu Thr Arg Ala Ile Ile Leu Pro Gly Val Ile Val Ser Gln Gly 195 200 205 Thr Thr Val Ala Ala Asn Ala Ile Val Thr Lys Ser Phe Pro Lys His 210 215 220 Ser Leu Ile Gly Gly Val Pro Ala Arg Tyr Ile Gly Gln Ser Asn Lys 225 230 235 240 Glu Ile Ser Val Lys Glu Gln Lys Asn Ile Val Asp Ile Leu Lys 245 250 255 Glu Gly Leu Gly Thr Glu Pro Val Ile Lys Asn Asn Ser Phe Cys Phe 260 265 270 Glu Lys Phe Asn Glu Asn Ile Thr Phe Gln Tyr Asp Leu Glu Lys Ile 275 280 285 Glu Ser Thr Asp Asn Ile Ser Gln Arg Asp Ile Ile Ile Phe Tyr Gln 290 295 300 Gly Thr Asn Lys Cys His Lys Asn Tyr Ser Thr Cys Ile Asp Leu Lys 305 310 315 320 Ser Lys Thr Ile Ser Gly Arg Ala Ser Lys Ala Ser Glu Phe Leu Arg 325 330 335 Asp Lys Phe Arg Arg Lys Gly Ile Ile Leu Asn Tyr Lys Asn Tyr Ser 340 345 350 Pro Phe Ser Leu Asn Tyr Asp Tyr Leu Ile Ile Asn Lys Ile Glu Val 355 360 365 <210> 89 <211> 219 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (219) <223> Streptococcus pneumoniae spr0972 gene <400> 89 atgtacagac gtttgagaga tttgagggaa gatcacgatt tgacccaaaa acaaatagct 60 aaaatacttt cgtttacaaa ctcagcttat gctaaaattg aacgaggtga gcatacgtta 120 acagcagatg tattggtaac tctctcaaac ttttacgatg tcagtacaga ctacctattg 180 gaattgacag attttcctga taaaattcgc tttagaaaa 219 <210> 90 <211> 73 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (73) <223> Conserved hypothetical protein <400> 90 Met Tyr Arg Arg Leu Arg Asp Leu Arg Glu Asp His Asp Leu Thr Gln 1 5 10 15 Lys Gln Ile Ala Lys Ile Leu Ser Phe Thr Asn Ser Ala Tyr Ala Lys 20 25 30 Ile Glu Arg Gly Glu His Thr Leu Thr Ala Asp Val Leu Val Thr Leu 35 40 45 Ser Asn Phe Tyr Asp Val Ser Thr Asp Tyr Leu Leu Glu Leu Thr Asp 50 55 60 Phe Pro Asp Lys Ile Arg Phe Arg Lys 65 70 <210> 91 <211> 1773 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1773) <223> Streptococcus pneumoniae spr0978 gene <400> 91 atggaggtat tgtgtatggt tgacaaacaa gtcattgaag aaatcaaaaa caatgccaac 60 attgtggaag tcataggaga tgtgatttct ttacaaaagg caggacggaa ctatctaggg 120 ctctgtcctt ttcatggtga aaaaacacct tctttcagcg ttgtagagga caagcagttt 180 taccactgtt ttggttgtgg tcgctcaggt gatgtcttta aattcatcga ggagtaccaa 240 ggggttacct ttatggaggc tgtccaaatc ttaggtcagc gtgtcgggat tgaggttgaa 300 aaaccgcttt atagtgaaca gaagccagcc tcgcctcacc aagctcttta tgatatgcac 360 gaagatgcgg ctaaatttta ccatgctatt ctcatgacaa cgactatggg cgaagaggcc 420 agaaattacc tttatcagcg gggtttgaca gatgaagtgc ttaaacattt ttggattggt 480 ttagcacctc cagaacgaaa ctatctctat caacgtttgt ctgatcagta tcgtgaagag 540 gatttactgg attcaggcct gttttatctt tcggatgcca atcaatttgt agacaccttt 600 cacaatcgca ttatgtttcc cctgacaaat gaccaaggaa aggtcattgc cttctcaggt 660 cgtatctggc aaaaaacgga ttcacaaact tctaagtata aaaacagccg atcgactgta 720 atttttaaca aaagttacga attatatcat atggataggg caaaaagatc ttctggaaaa 780 gctagtgaga tttacctgat ggaaggattc atggatgtta ttgcagccta tcgggctgga 840 atcgaaaatg ctgtggcgtc gatgggaacg gccttgagtc gagagcatgt tgagcatctg 900 aaaaggttaa ccaagaaatt ggttcttgtt tacgatggag ataaggctgg gcaagccgcg 960 acattgaaag cattggatga aattggtgat atgcctgtgc aaatcgtcag catgcctgat 1020 aacttggatc ctgatgaata tctacaaaaa aatggtccag aagacttggc ctatctatta 1080 acgaaaactc gtattagtcc gattgagttc tacattcatc agtacaaacc tgaaaacggt 1140 gaaaatctgc aggctcagat tgagtttctt gaaaaaatag ctcccttgat tgttcaagaa 1200 aagtccatcg ctgctcaaaa cagctatatt catattttag ctgacagtct ggcgtccttt 1260 gattataccc agattgagca gattgttaat gagagtcgtc aggtgcaaag gcagaatcgc 1320 atggaaagaa tttccagacc gacgccaatc accatgcctg tcaccaagca gttatcggct 1380 attatgaggg cagaagccca tctactctat cggatgatgg aatcccctct tgttttgaac 1440 gattaccgtt tgcgagaaga ctttgcattt gctacacctg aatttcaggt cttacatgac 1500 ttgcttggcc agtatggaaa tcttcctcca gaagttttag cagagcagac agaggaagtt 1560 gaaagagctt ggtaccaagt tttagctcag gatttgcctg ctgagatatc gccgcaggaa 1620 cttagtgaag tagagatgac tcgaaacaag gctctcttga atcaggacaa tatgagaatc 1680 aaaaagaagg tgcaggaagc tagccatgta ggagatacag atacagccct agaagaattg 1740 gaacgtttaa tttcccaaaa gagaagaatg gag 1773 <210> 92 <211> 591 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (591) <223> DNA primase <400> 92 Met Glu Val Leu Cys Met Val Asp Lys Gln Val Ile Glu Glu Ile Lys 1 5 10 15 Asn Asn Ala Asn Ile Val Glu Val Ile Gly Asp Val Ile Ser Leu Gln 20 25 30 Lys Ala Gly Arg Asn Tyr Leu Gly Leu Cys Pro Phe His Gly Glu Lys 35 40 45 Thr Pro Ser Phe Ser Val Val Glu Asp Lys Gln Phe Tyr His Cys Phe 50 55 60 Gly Cys Gly Arg Ser Gly Asp Val Phe Lys Phe Ile Glu Glu Tyr Gln 65 70 75 80 Gly Val Thr Phe Met Glu Ala Val Gln Ile Leu Gly Gln Arg Val Gly 85 90 95 Ile Glu Val Glu Lys Pro Leu Tyr Ser Glu Gln Lys Pro Ala Ser Pro 100 105 110 His Gln Ala Leu Tyr Asp Met His Glu Asp Ala Ala Lys Phe Tyr His 115 120 125 Ala Ile Leu Met Thr Thr Thr Met Gly Glu Glu Ala Arg Asn Tyr Leu 130 135 140 Tyr Gln Arg Gly Leu Thr Asp Glu Val Leu Lys His Phe Trp Ile Gly 145 150 155 160 Leu Ala Pro Pro Glu Arg Asn Tyr Leu Tyr Gln Arg Leu Ser Asp Gln 165 170 175 Tyr Arg Glu Glu Asp Leu Leu Asp Ser Gly Leu Phe Tyr Leu Ser Asp 180 185 190 Ala Asn Gln Phe Val Asp Thr Phe His Asn Arg Ile Met Phe Pro Leu 195 200 205 Thr Asn Asp Gln Gly Lys Val Ile Ala Phe Ser Gly Arg Ile Trp Gln 210 215 220 Lys Thr Asp Ser Gln Thr Ser Lys Tyr Lys Asn Ser Arg Ser Thr Val 225 230 235 240 Ile Phe Asn Lys Ser Tyr Glu Leu Tyr His Met Asp Arg Ala Lys Arg 245 250 255 Ser Ser Gly Lys Ala Ser Glu Ile Tyr Leu Met Glu Gly Phe Met Asp 260 265 270 Val Ile Ala Ala Tyr Arg Ala Gly Ile Glu Asn Ala Val Ala Ser Met 275 280 285 Gly Thr Ala Leu Ser Arg Glu His Val Glu His Leu Lys Arg Leu Thr 290 295 300 Lys Lys Leu Val Leu Val Tyr Asp Gly Asp Lys Ala Gly Gln Ala Ala 305 310 315 320 Thr Leu Lys Ala Leu Asp Glu Ile Gly Asp Met Pro Val Gln Ile Val 325 330 335 Ser Met Pro Asp Asn Leu Asp Pro Asp Glu Tyr Leu Gln Lys Asn Gly 340 345 350 Pro Glu Asp Leu Ala Tyr Leu Leu Thr Lys Thr Arg Ile Ser Pro Ile 355 360 365 Glu Phe Tyr Ile His Gln Tyr Lys Pro Glu Asn Gly Glu Asn Leu Gln 370 375 380 Ala Gln Ile Glu Phe Leu Glu Lys Ile Ala Pro Leu Ile Val Gln Glu 385 390 395 400 Lys Ser Ile Ala Ala Gln Asn Ser Tyr Ile His Ile Leu Ala Asp Ser 405 410 415 Leu Ala Ser Phe Asp Tyr Thr Gln Ile Glu Gln Ile Val Asn Glu Ser 420 425 430 Arg Gln Val Gln Arg Gln Asn Arg Met Glu Arg Ile Ser Arg Pro Thr 435 440 445 Pro Ile Thr Met Pro Val Thr Lys Gln Leu Ser Ala Ile Met Arg Ala 450 455 460 Glu Ala His Leu Leu Tyr Arg Met Met Glu Ser Pro Leu Val Leu Asn 465 470 475 480 Asp Tyr Arg Leu Arg Glu Asp Phe Ala Phe Ala Thr Pro Glu Phe Gln 485 490 495 Val Leu His Asp Leu Leu Gly Gln Tyr Gly Asn Leu Pro Pro Glu Val 500 505 510 Leu Ala Glu Gln Thr Glu Glu Val Glu Arg Ala Trp Tyr Gln Val Leu 515 520 525 Ala Gln Asp Leu Pro Ala Glu Ile Ser Pro Gln Glu Leu Ser Glu Val 530 535 540 Glu Met Thr Arg Asn Lys Ala Leu Leu Asn Gln Asp Asn Met Arg Ile 545 550 555 560 Lys Lys Lys Val Gln Glu Ala Ser His Val Gly Asp Thr Asp Thr Ala 565 570 575 Leu Glu Glu Leu Glu Arg Leu Ile Ser Gln Lys Arg Arg Met Glu 580 585 590 <210> 93 <211> 1107 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1107) <223> Streptococcus pneumoniae spr0979 gene <400> 93 atggcaacaa aacaaaaaga agtaacaaca tttgacgtac aggtagcaga atttatccgt 60 aatcataagc aaaaagggac agcaacagat gatgaaatca atgcaagtct ggttattcct 120 tttaccttgg acgctgatgg gattgaagat ctcttgcaac ggattcagga tgcagggatt 180 tctatcacag ataacgaagg aaatccaagt gcgcgtgttc ttagcaatga agaagaacca 240 gaactcagcg atgaggactt gattgggtca acttctgcta aggtcaatga ccctgtccgt 300 atgtacttga aagaaatagg ggtcgttcct ctcttgacca atgaagagga gaaagagttg 360 gcactggctg ttgaagctgg tgatatcgaa gccaaacaac gtcttgcgga agccaatctt 420 cgtttggttg tttccattgc caaacgctat gtcggtcgtg gtatgcagtt ccttgacttg 480 attcaagaag gaaatatggg cttgatgaag gcggttgaca agtttgacta ttctaaaggg 540 ttcaagtttt caacttatgc aacttggtgg attcgtcagg ctatcactcg tgctattgcg 600 gaccaagctc gtaccatccg tatcccagtt cacatggttg aaactatcaa taaattggtt 660 cgtgaacagc ggaatctcct tcaagaattg gggcaagatc cgacaccaga acagattgct 720 gaacgaatgg atatgacacc tgataaggtt cgtgaaatct tgaagattgc ccaagaacca 780 gtatctcttg aaactcctat cggtgaagag gacgatagcc accttggaga ctttatcgaa 840 gatgaagtga ttgaaaatcc agtggattat acgactcgta tcgtcttgcg tgagcaattg 900 gatgaaatct tagatactct tacagaccgt gaagaaaatg ttctgcgtct acgttttgga 960 ctagatgatg gaaaaatgcg cacacttgaa gatgtgggga aagtctttaa cgtaactcgt 1020 gagcgtatcc gtcagattga agcaaaggct ttgagaaaac tacgccaacc aagtcgtagc 1080 aaaccgcttc gtgattttat tgaagac 1107 <210> 94 <211> 369 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (369) RNA polymerase sigma factor 70 <400> 94 Met Ala Thr Lys Gln Lys Glu Val Thr Thr Phe Asp Val Gln Val Ala 1 5 10 15 Glu Phe Ile Arg Asn His Lys Gln Lys Gly Thr Ala Thr Asp Asp Glu 20 25 30 Ile Asn Ala Ser Leu Val Ile Pro Phe Thr Leu Asp Ala Asp Gly Ile 35 40 45 Glu Asp Leu Leu Gln Arg Ile Gln Asp Ala Gly Ile Ser Ile Thr Asp 50 55 60 Asn Glu Gly Asn Pro Ser Ala Arg Val Leu Ser Asn Glu Glu Glu Pro 65 70 75 80 Glu Leu Ser Asp Glu Asp Leu Ile Gly Ser Thr Ser Ala Lys Val Asn 85 90 95 Asp Pro Val Arg Met Tyr Leu Lys Glu Ile Gly Val Val Pro Leu Leu 100 105 110 Thr Asn Glu Glu Glu Lys Glu Leu Ala Leu Ala Val Glu Ala Gly Asp 115 120 125 Ile Glu Ala Lys Gln Arg Leu Ala Glu Ala Asn Leu Arg Leu Val Val 130 135 140 Ser Ile Ala Lys Arg Tyr Val Gly Arg Gly Met Gln Phe Leu Asp Leu 145 150 155 160 Ile Gln Glu Gly Asn Met Gly Leu Met Lys Ala Val Asp Lys Phe Asp 165 170 175 Tyr Ser Lys Gly Phe Lys Phe Ser Thr Tyr Ala Thr Trp Trp Ile Arg 180 185 190 Gln Ala Ile Thr Arg Ala Ile Ala Asp Gln Ala Arg Thr Ile Arg Ile 195 200 205 Pro Val His Met Val Glu Thr Ile Asn Lys Leu Val Arg Glu Gln Arg 210 215 220 Asn Leu Leu Gln Glu Leu Gly Gln Asp Pro Thr Pro Glu Gln Ile Ala 225 230 235 240 Glu Arg Met Asp Met Thr Pro Asp Lys Val Arg Glu Ile Leu Lys Ile 245 250 255 Ala Gln Glu Pro Val Ser Leu Glu Thr Pro Ile Gly Glu Glu Asp Asp 260 265 270 Ser His Leu Gly Asp Phe Ile Glu Asp Glu Val Ile Glu Asn Pro Val 275 280 285 Asp Tyr Thr Thr Arg Ile Val Leu Arg Glu Gln Leu Asp Glu Ile Leu 290 295 300 Asp Thr Leu Thr Asp Arg Glu Glu Asn Val Leu Arg Leu Arg Phe Gly 305 310 315 320 Leu Asp Asp Gly Lys Met Arg Thr Leu Glu Asp Val Gly Lys Val Phe 325 330 335 Asn Val Thr Arg Glu Arg Ile Arg Gln Ile Glu Ala Lys Ala Leu Arg 340 345 350 Lys Leu Arg Gln Pro Ser Arg Ser Lys Pro Leu Arg Asp Phe Ile Glu 355 360 365 Asp <210> 95 <211> 1323 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1323) <223> Streptococcus pneumoniae spr0982 gene <400> 95 atgcgaattg gtttatttac agatacctat tttcctcagg tttctggtgt tgcgaccagt 60 attcgaacct tgaaaacaga acttgaaaag cagggacatg ctgtttttat ctttacgacg 120 acagataagg atgtcaatcg ctacgaagat tggcaaatta tccgcattcc aagtgttcct 180 ttctttgctt ttaaggatcg tcgctttgcc taccgaggtt ttagcaaggc acttgaaatt 240 gctaaacagt atcagctaga tattatccat actcagacag aattttctct tggcctgttg 300 gggatttgga ttgcgcgtga attgaaaatt ccagtcatcc atacctatca cacccagtat 360 gaagactatg tccattatat tgctaagggg atgttgatcc ggccgagtat ggtcaagtat 420 ctggttagag gtttcctgca tgatgtggat ggggttattt gccctagtga gattgtccgt 480 gacttgctat ctgattataa ggtcaaggtt gaaaaacggg tcattcctac tgggattgaa 540 ttagccaagt ttgagcgtcc ggaaatcaag caggaaaatt tgaaagaact gcgtagtaaa 600 ctagggattc aagatggtga aaagacgttg cttagtcttt cgagaatctc ctatgaaaaa 660 aatattcaag cagttttagt agcctttgct gatgttctga aagaggaaga caaggttaaa 720 ctggtagtag ctggggatgg cccttatctg aatgacctca aagagcaagc ccagaaccta 780 gagattcaag actcagtcat ctttacaggg atgattgctc ctagtgagac ggctctttac 840 tataaagcgg cggatttctt catttcggca tcgacaagcg aaacgcaagg tttgacctac 900 ttggaaagct tagccagtgg aacacctgtc attgctcacg gaaatcctta tttgaacaac 960 ctcatcagtg ataaaatgtt tggagccttg tactatggag aacatgattt ggctggtgct 1020 attttggaag ccctgattgc aacaccagac atgaatgagc ataccttatc agagaaattg 1080 tatgagattt cagctgagaa ctttgggaaa cgagtgcatg agttttatct ggatgccatt 1140 atttcaaata acttccagaa agatttggct aaagatgata cggtcagtca gcgtatcttt 1200 aagacagttt tgtatcttcc gcaacaggtg gttgctgtac ctgtaaaagg atctagacgc 1260 atgttgaagg cttcaaaaac acagttgatc agtatgagag actattggaa agaccatgaa 1320 gaa 1323 <210> 96 <211> 441 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (441) <223> Conserved Hypothetical protein <400> 96 Met Arg Ile Gly Leu Phe Thr Asp Thr Tyr Phe Pro Gln Val Ser Gly 1 5 10 15 Val Ala Thr Ser Ile Arg Thr Leu Lys Thr Glu Leu Glu Lys Gln Gly 20 25 30 His Ala Val Phe Ile Phe Thr Thr Thr Asp Lys Asp Val Asn Arg Tyr 35 40 45 Glu Asp Trp Gln Ile Ile Arg Ile Pro Ser Val Pro Phe Phe Ala Phe 50 55 60 Lys Asp Arg Arg Phe Ala Tyr Arg Gly Phe Ser Lys Ala Leu Glu Ile 65 70 75 80 Ala Lys Gln Tyr Gln Leu Asp Ile Ile His Thr Gln Thr Glu Phe Ser 85 90 95 Leu Gly Leu Leu Gly Ile Trp Ile Ala Arg Glu Leu Lys Ile Pro Val 100 105 110 Ile His Thr Tyr His Thr Gln Tyr Glu Asp Tyr Val His Tyr Ile Ala 115 120 125 Lys Gly Met Leu Ile Arg Pro Ser Met Val Lys Tyr Leu Val Arg Gly 130 135 140 Phe Leu His Asp Val Asp Gly Val Ile Cys Pro Ser Glu Ile Val Arg 145 150 155 160 Asp Leu Leu Ser Asp Tyr Lys Val Lys Val Glu Lys Arg Val Ile Pro 165 170 175 Thr Gly Ile Glu Leu Ala Lys Phe Glu Arg Pro Glu Ile Lys Gln Glu 180 185 190 Asn Leu Lys Glu Leu Arg Ser Lys Leu Gly Ile Gln Asp Gly Glu Lys 195 200 205 Thr Leu Leu Ser Leu Ser Arg Ile Ser Tyr Glu Lys Asn Ile Gln Ala 210 215 220 Val Leu Val Ala Phe Ala Asp Val Leu Lys Glu Glu Asp Lys Val Lys 225 230 235 240 Leu Val Val Ala Gly Asp Gly Pro Tyr Leu Asn Asp Leu Lys Glu Gln 245 250 255 Ala Gln Asn Leu Glu Ile Gln Asp Ser Val Ile Phe Thr Gly Met Ile 260 265 270 Ala Pro Ser Glu Thr Ala Leu Tyr Tyr Lys Ala Ala Asp Phe Phe Ile 275 280 285 Ser Ala Ser Thr Ser Glu Thr Gln Gly Leu Thr Tyr Leu Glu Ser Leu 290 295 300 Ala Ser Gly Thr Pro Val Ile Ala His Gly Asn Pro Tyr Leu Asn Asn 305 310 315 320 Leu Ile Ser Asp Lys Met Phe Gly Ala Leu Tyr Tyr Gly Glu His Asp 325 330 335 Leu Ala Gly Ala Ile Leu Glu Ala Leu Ile Ala Thr Pro Asp Met Asn 340 345 350 Glu His Thr Leu Ser Glu Lys Leu Tyr Glu Ile Ser Ala Glu Asn Phe 355 360 365 Gly Lys Arg Val His Glu Phe Tyr Leu Asp Ala Ile Ile Ser Asn Asn 370 375 380 Phe Gln Lys Asp Leu Ala Lys Asp Asp Thr Val Ser Gln Arg Ile Phe 385 390 395 400 Lys Thr Val Leu Tyr Leu Pro Gln Gln Val Val Ala Val Pro Val Lys 405 410 415 Gly Ser Arg Arg Met Leu Lys Ala Ser Lys Thr Gln Leu Ile Ser Met 420 425 430 Arg Asp Tyr Trp Lys Asp His Glu Glu 435 440 <210> 97 <211> 858 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (858) <223> Streptococcus pneumoniae spr0992 gene <400> 97 atgataacat taaaatcagc tcgtgaaatc gaagctatgg acaaggctgg tgattttcta 60 gcaagtattc atataggctt acgtgatttg attaagccag gcgtagatat gtgggaagtt 120 gaagaatatg tccgccgtcg ttgtaaagaa gaaaatttcc ttccacttca gattggggtt 180 gacggtgcca tgatggacta tccttatgct acctgttgct ctcttaacga tgaagtggct 240 cacgctttcc ctcgtcatta tatcttgaaa gatggtgatt tgctcaaagt tgatatggtt 300 ttgggaggtc ccattgctaa atctgaccta aatgtctcaa aattaaactt caacaatgtt 360 gaacaaatga aaaaatacac tcagagctat tctggtggtt tagcagactc atgttgggct 420 tatgctgttg gtacaccgtc cgaagaagtc aaaaacttga tggatgtaac caaagaagct 480 atgtacaagg gtattgagca agctgttgtt ggaaatcgta tcggtgatat cggtgcggct 540 attcaagaat acgctgaaag tcgtggttac ggtgtagtgc gtgatttggt tggtcatggt 600 gttggcccaa ctatgcacga agaaccaatg gttcctaact atggtattgc aggtcgtgga 660 ctccgtcttc gtgaaggaat ggtcttaacc attgaaccaa tgatcaatac aggcgattgg 720 gaaattgata cagatatgaa aactggttgg gcgcataaga ccattgacgg tggattgtca 780 tgtcagtatg aacaccaatt tgtcattacg aaagatggac ctgttatctt gactagccaa 840 ggtgaagaag gaacttat 858 <210> 98 <211> 286 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (286) <223> Methionine aminopeptidase <400> 98 Met Ile Thr Leu Lys Ser Ala Arg Glu Ile Glu Ala Met Asp Lys Ala 1 5 10 15 Gly Asp Phe Leu Ala Ser Ile His Ile Gly Leu Arg Asp Leu Ile Lys 20 25 30 Pro Gly Val Asp Met Trp Glu Val Glu Glu Tyr Val Arg Arg Arg Cys 35 40 45 Lys Glu Glu Asn Phe Leu Pro Leu Gln Ile Gly Val Asp Gly Ala Met 50 55 60 Met Asp Tyr Pro Tyr Ala Thr Cys Cys Ser Leu Asn Asp Glu Val Ala 65 70 75 80 His Ala Phe Pro Arg His Tyr Ile Leu Lys Asp Gly Asp Leu Leu Lys 85 90 95 Val Asp Met Val Leu Gly Gly Pro Ile Ala Lys Ser Asp Leu Asn Val 100 105 110 Ser Lys Leu Asn Phe Asn Asn Val Glu Gln Met Lys Lys Tyr Thr Gln 115 120 125 Ser Tyr Ser Gly Gly Leu Ala Asp Ser Cys Trp Ala Tyr Ala Val Gly 130 135 140 Thr Pro Ser Glu Glu Val Lys Asn Leu Met Asp Val Thr Lys Glu Ala 145 150 155 160 Met Tyr Lys Gly Ile Glu Gln Ala Val Val Gly Asn Arg Ile Gly Asp 165 170 175 Ile Gly Ala Ala Ile Gln Glu Tyr Ala Glu Ser Arg Gly Tyr Gly Val 180 185 190 Val Arg Asp Leu Val Gly His Gly Val Gly Pro Thr Met His Glu Glu 195 200 205 Pro Met Val Pro Asn Tyr Gly Ile Ala Gly Arg Gly Leu Arg Leu Arg 210 215 220 Glu Gly Met Val Leu Thr Ile Glu Pro Met Ile Asn Thr Gly Asp Trp 225 230 235 240 Glu Ile Asp Thr Asp Met Lys Thr Gly Trp Ala His Lys Thr Ile Asp 245 250 255 Gly Gly Leu Ser Cys Gln Tyr Glu His Gln Phe Val Ile Thr Lys Asp 260 265 270 Gly Pro Val Ile Leu Thr Ser Gln Gly Glu Glu Gly Thr Tyr 275 280 285 <210> 99 <211> 693 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (693) <223> Streptococcus pneumoniae spr0996 gene <400> 99 gtgaggagga tttttatgta cagtatttca ttccaagaag attcactatt accaagagaa 60 aggctggcca aggaaggagt tgaagcgctt agtaaccaag agttgctagc tattttactc 120 aggacaggaa cacgtcaagc tagcgttttt gaaattgccc aaaaagtctt gaacaatctt 180 tcaagcctaa cggatttgaa aaaaatgacc ctgcaggaat tgcagagttt gtctggtatt 240 gggcgtgtta aggccataga attacaagct atgattgaac tggggcatcg tattcacaaa 300 cacgagactc ttgaaatgga aagtattctc agcagtcaaa agttggccaa gaagatgcag 360 caggaattag gggataaaaa acaagagcac ctggtggcac tctatctcaa tactcaaaat 420 caaatcatcc atcagcagac catttttatc gggtctgtaa ctcgtagtat cgctgaaccg 480 cgagagattc ttcactatgc aatcaagcat atggcgactt ctcttgtctt ggtccacaat 540 catccttcag gagcggtagc gcctagccaa aatgatgatc atgtcactaa acttgttaaa 600 gaagcctgcg aactgatggg gattgttctc ttggaccatt tgattgtctc tcattctaat 660 tactttagtt atcgtgaaaa gacagattta atc 693 <210> 100 <211> 231 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (231) <223> DNA repair protein <400> 100 Met Arg Arg Ile Phe Met Tyr Ser Ile Ser Phe Gln Glu Asp Ser Leu 1 5 10 15 Leu Pro Arg Glu Arg Leu Ala Lys Glu Gly Val Glu Ala Leu Ser Asn 20 25 30 Gln Glu Leu Leu Ala Ile Leu Leu Arg Thr Gly Thr Arg Gln Ala Ser 35 40 45 Val Phe Glu Ile Ala Gln Lys Val Leu Asn Asn Leu Ser Ser Leu Thr 50 55 60 Asp Leu Lys Lys Met Thr Leu Gln Glu Leu Gln Ser Leu Ser Gly Ile 65 70 75 80 Gly Arg Val Lys Ala Ile Glu Leu Gln Ala Met Ile Glu Leu Gly His 85 90 95 Arg Ile His Lys His Glu Thr Leu Glu Met Glu Ser Ile Leu Ser Ser 100 105 110 Gln Lys Leu Ala Lys Lys Met Gln Gln Glu Leu Gly Asp Lys Lys Gln 115 120 125 Glu His Leu Val Ala Leu Tyr Leu Asn Thr Gln Asn Gln Ile Ile His 130 135 140 Gln Gln Thr Ile Phe Ile Gly Ser Val Thr Arg Ser Ile Ala Glu Pro 145 150 155 160 Arg Glu Ile Leu His Tyr Ala Ile Lys His Met Ala Thr Ser Leu Val 165 170 175 Leu Val His Asn His Pro Ser Gly Ala Val Ala Pro Ser Gln Asn Asp 180 185 190 Asp His Val Thr Lys Leu Val Lys Glu Ala Cys Glu Leu Met Gly Ile 195 200 205 Val Leu Leu Asp His Leu Ile Val Ser His Ser Asn Tyr Phe Ser Tyr 210 215 220 Arg Glu Lys Thr Asp Leu Ile 225 230 <210> 101 <211> 1113 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1001) <223> Streptococcus pneumoniae spr1001 gene <400> 101 ttgatttatt tggacaatgc tgcaacgact cctatgtcag cagttgctat ttcagctatg 60 accaaggtta tgcaagaaac ccacggaaat ccttctagta ttcatggtca tggtcgtcaa 120 gctggcaaac tcttgcgaga agcccgtcag gaactagccc agttactaag gacaaaacct 180 caacatatct ttttcacttc tggtgggact gaagccaata atactaccat cattggctac 240 tgtcttcgtc accaagaaca aggaaaacat atcatcacaa ctgccatcga gcaccatgct 300 gtccttgaaa caattgatta cttggttcaa cactttgggt ttgaagcaac cattatccag 360 ccagaaaatc aagaaatcac agcccagcaa attcaaaagg ctttacgtga cgatacgatt 420 ttggtttcta ccatgtttgc caataatgag acaggaaacc tactgcccat cgctgaaatt 480 ggccaaatac tcaagcaaca ccctgctgcc tatcatgttg atgcagttca ggctattggt 540 aaaatcccaa ttcattcaga agaattgggc attgattttc tcactgcttc tgcccacaaa 600 ttccatggtc ctaagggaat cggttttctc tacgcatcta gcatggactt tgattcctat 660 ctacatggcg gagaccagga acagaaaaaa cgtgcaggaa ctgaaaatct gcctgccatc 720 gtaggcatgg ttgcagccct aaaagaaaac ctagaaaaac aagaagaaca ttttcaacat 780 gtacaaaatc tagaaactgc ctttctggca gagctggagg gcattcagta ttacttgaat 840 agaggaaaac atcatctccc ttatgttctc aatattggat ttcctggtca gaaaaatgac 900 ctcttactcc ttcggctaga tttagctgga atttcaatct ctactggctc agcctgtact 960 gcaggcgttg tccaatccag ccatgttctt gaagccatgt atggcgcaaa ttcagaacgc 1020 ttgaaggaat cccttcgcat cagtttgtcg ccacaaaata ccgttgaaga cctacaaacc 1080 ctcgcaaaaa ccttaaaaga aattatcgga ggt 1113 <210> 102 <211> 371 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (371) Pyridoxal-phosphate dependent aminotransferase <400> 102 Met Ile Tyr Leu Asp Asn Ala Ala Thr Thr Pro Met Ser Ala Val Ala 1 5 10 15 Ile Ser Ala Met Thr Lys Val Met Gln Glu Thr His Gly Asn Pro Ser 20 25 30 Ser Ile His Gly His Gly Arg Gln Ala Gly Lys Leu Leu Arg Glu Ala 35 40 45 Arg Gln Glu Leu Ala Gln Leu Leu Arg Thr Lys Pro Gln His Ile Phe 50 55 60 Phe Thr Ser Gly Gly Thr Glu Ala Asn Asn Thr Thr Ile Ily Gly Tyr 65 70 75 80 Cys Leu Arg His Gln Glu Gln Gly Lys His Ile Ile Thr Thr Ala Ile 85 90 95 Glu His His Ala Val Leu Glu Thr Ile Asp Tyr Leu Val Gln His Phe 100 105 110 Gly Phe Glu Ala Thr Ile Ile Gln Pro Glu Asn Gln Glu Ile Thr Ala 115 120 125 Gln Gln Ile Gln Lys Ala Leu Arg Asp Asp Thr Ile Leu Val Ser Thr 130 135 140 Met Phe Ala Asn Asn Glu Thr Gly Asn Leu Leu Pro Ile Ala Glu Ile 145 150 155 160 Gly Gln Ile Leu Lys Gln His Pro Ala Ala Tyr His Val Asp Ala Val 165 170 175 Gln Ala Ile Gly Lys Ile Pro Ile His Ser Glu Glu Leu Gly Ile Asp 180 185 190 Phe Leu Thr Ala Ser Ala His Lys Phe His Gly Pro Lys Gly Ile Gly 195 200 205 Phe Leu Tyr Ala Ser Ser Met Asp Phe Asp Ser Tyr Leu His Gly Gly 210 215 220 Asp Gln Glu Gln Lys Lys Arg Ala Gly Thr Glu Asn Leu Pro Ala Ile 225 230 235 240 Val Gly Met Val Ala Ala Leu Lys Glu Asn Leu Glu Lys Gln Glu Glu 245 250 255 His Phe Gln His Val Gln Asn Leu Glu Thr Ala Phe Leu Ala Glu Leu 260 265 270 Glu Gly Ile Gln Tyr Tyr Leu Asn Arg Gly Lys His His Leu Pro Tyr 275 280 285 Val Leu Asn Ile Gly Phe Pro Gly Gln Lys Asn Asp Leu Leu Leu Leu 290 295 300 Arg Leu Asp Leu Ala Gly Ile Ser Ile Ser Thr Gly Ser Ala Cys Thr 305 310 315 320 Ala Gly Val Val Gln Ser Ser His Val Leu Glu Ala Met Tyr Gly Ala 325 330 335 Asn Ser Glu Arg Leu Lys Glu Ser Leu Arg Ile Ser Leu Ser Pro Gln 340 345 350 Asn Thr Val Glu Asp Leu Gln Thr Leu Ala Lys Thr Leu Lys Glu Ile 355 360 365 Ile Gly Gly 370 <210> 103 <211> 1956 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1956) <223> Streptococcus pneumoniae spr1024 gene <400> 103 atgaataaaa gaatgaatga gttagtcgct ttgctcaatc gctatgcgac tgagtactat 60 accagcgata atccctcggt ttcagacagt gagtatgacc gcctttaccg tgagttggtc 120 gagttagaaa ctgcttatcc agagcaagtg ctagcagaca gtccgactca tcgtgttggt 180 ggcaaggttt tagatggttt tgaaaaatac agtcatcagt atcctcttta tagtttgcag 240 gatgcttttt cacgtgagga gctagatgct tttgatgcgc gtgttcgtaa ggaagtggct 300 catccgacct atatttgtga gctgaaaatc gatggcttat ctatctcgct gacttatgaa 360 aaggggattt tggttgctgg ggtaacacgt ggagatggtt caattggtga aaatatcaca 420 gaaaacctca agcgtgttaa ggacatccct ttgactttgc cagaagaact agatatcaca 480 gttcgtgggg aatgttacat gccacgcgct tcctttgacc aagttaacca agcgcgccaa 540 gaaaatggag agcctgaatt tgctaatcct cgtaatgcgg cagcaggaac tctgcgtcag 600 ttggatacag cagtagttgc caagcgtaat cttgcaacgt ttctctatca agaagccagc 660 ccttcaactc gtgatagcca agaaaagggt ttgaagtacc tagaacaact aggttttgtg 720 gtcaatccta agcgaatctt ggctgaaaac atagatgaaa tctggaattt tatccaagaa 780 gtaggacagg aacgggaaaa tctgccttac gatattgatg gagtggtaat caaggtcaac 840 gacctagcaa gtcaagaaga acttggtttt accgttaagg ctccaaagtg ggcagtagcc 900 tacaagttcc ctgctgaaga aaaagaagct caactcttat cagttgactg gacagttggc 960 cgtaccggtg ttgtaactcc aactgctaat ctaacaccag tacaacttgc cggtacgact 1020 gttagccgtg cgaccctgca caatgtagat tatattgctg aaaaagatat ccgaaaagac 1080 gatacggtca ttgtatataa ggctggtgac atcatccctg ccgttttacg tgtggtagag 1140 tccaaacggg tttctgaaga aaaactagat atccctacaa actgtccaag ttgtaactct 1200 gacttgttgc actttgaaga tgaagtggcc ctacgttgta tcaatccgcg ttgccctgct 1260 caaatcatgg aaggcttgat tcactttgct tctcgtgatg ctatgaatat tacaggcctt 1320 ggtccatcta ttgttgagaa gctttttgct gctaatttag tcaaggatgt ggcggatatt 1380 tatcgtttgc aagaagagga tttcctcctt ttagaggggg ttaaggaaaa gtccgctgct 1440 aaactgtatc aggctatcca agcatcaaag gaaaattctg ccgagaagct cttatttggt 1500 ttgggaattc gtcatgtcgg aagcaaggct agtcagcttt tacttcaata tttccattca 1560 attgaaaatc tgtatcaggc agattcagag gaagtggcta gtattgaaag tctaggtggc 1620 gtgattgcca aaagtcttca gacttatttt gcggcagaag gctctgaaat tctgctcaga 1680 gaattgaaag aaactggggt caatctggac tataaaggac agacggtagt agcggatgcg 1740 gccttgtcag gtttgaccgt ggtattgaca ggaaaattgg aacgactcaa gcgctcagaa 1800 gctaaaagta aactcgaaag tctgggtgcc aaagtgacag gtagtgtttc taaaaagacc 1860 gacctcgtcg tggtaggtgc agacgctgga agtaaactgc aaaaagcaca agaacttggt 1920 atccaggtca gagatgaggc atggctagaa agtttg 1956 <210> 104 <211> 652 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (652) <223> DNA ligase <400> 104 Met Asn Lys Arg Met Asn Glu Leu Val Ala Leu Leu Asn Arg Tyr Ala 1 5 10 15 Thr Glu Tyr Tyr Thr Ser Asp Asn Pro Ser Val Ser Asp Ser Glu Tyr 20 25 30 Asp Arg Leu Tyr Arg Glu Leu Val Glu Leu Glu Thr Ala Tyr Pro Glu 35 40 45 Gln Val Leu Ala Asp Ser Pro Thr His Arg Val Gly Gly Lys Val Leu 50 55 60 Asp Gly Phe Glu Lys Tyr Ser His Gln Tyr Pro Leu Tyr Ser Leu Gln 65 70 75 80 Asp Ala Phe Ser Arg Glu Glu Leu Asp Ala Phe Asp Ala Arg Val Arg 85 90 95 Lys Glu Val Ala His Pro Thr Tyr Ile Cys Glu Leu Lys Ile Asp Gly 100 105 110 Leu Ser Ile Ser Leu Thr Tyr Glu Lys Gly Ile Leu Val Ala Gly Val 115 120 125 Thr Arg Gly Asp Gly Ser Ile Gly Glu Asn Ile Thr Glu Asn Leu Lys 130 135 140 Arg Val Lys Asp Ile Pro Leu Thr Leu Pro Glu Glu Leu Asp Ile Thr 145 150 155 160 Val Arg Gly Glu Cys Tyr Met Pro Arg Ala Ser Phe Asp Gln Val Asn 165 170 175 Gln Ala Arg Gln Glu Asn Gly Glu Pro Glu Phe Ala Asn Pro Arg Asn 180 185 190 Ala Ala Ala Gly Thr Leu Arg Gln Leu Asp Thr Ala Val Val Ala Lys 195 200 205 Arg Asn Leu Ala Thr Phe Leu Tyr Gln Glu Ala Ser Pro Ser Thr Arg 210 215 220 Asp Ser Gln Glu Lys Gly Leu Lys Tyr Leu Glu Gln Leu Gly Phe Val 225 230 235 240 Val Asn Pro Lys Arg Ile Leu Ala Glu Asn Ile Asp Glu Ile Trp Asn 245 250 255 Phe Ile Gln Glu Val Gly Gln Glu Arg Glu Asn Leu Pro Tyr Asp Ile 260 265 270 Asp Gly Val Val Ile Lys Val Asn Asp Leu Ala Ser Gln Glu Glu Leu 275 280 285 Gly Phe Thr Val Lys Ala Pro Lys Trp Ala Val Ala Tyr Lys Phe Pro 290 295 300 Ala Glu Glu Lys Glu Ala Gln Leu Leu Ser Val Asp Trp Thr Val Gly 305 310 315 320 Arg Thr Gly Val Val Thr Pro Thr Ala Asn Leu Thr Pro Val Gln Leu 325 330 335 Ala Gly Thr Thr Val Ser Arg Ala Thr Leu His Asn Val Asp Tyr Ile 340 345 350 Ala Glu Lys Asp Ile Arg Lys Asp Asp Thr Val Ile Val Tyr Lys Ala 355 360 365 Gly Asp Ile Ile Pro Ala Val Leu Arg Val Val Glu Ser Lys Arg Val 370 375 380 Ser Glu Glu Lys Leu Asp Ile Pro Thr Asn Cys Pro Ser Cys Asn Ser 385 390 395 400 Asp Leu Leu His Phe Glu Asp Glu Val Ala Leu Arg Cys Ile Asn Pro 405 410 415 Arg Cys Pro Ala Gln Ile Met Glu Gly Leu Ile His Phe Ala Ser Arg 420 425 430 Asp Ala Met Asn Ile Thr Gly Leu Gly Pro Ser Ile Val Glu Lys Leu 435 440 445 Phe Ala Ala Asn Leu Val Lys Asp Val Ala Asp Ile Tyr Arg Leu Gln 450 455 460 Glu Glu Asp Phe Leu Leu Leu Glu Gly Val Lys Glu Lys Ser Ala Ala 465 470 475 480 Lys Leu Tyr Gln Ala Ile Gln Ala Ser Lys Glu Asn Ser Ala Glu Lys 485 490 495 Leu Leu Phe Gly Leu Gly Ile Arg His Val Gly Ser Lys Ala Ser Gln 500 505 510 Leu Leu Leu Gln Tyr Phe His Ser Ile Glu Asn Leu Tyr Gln Ala Asp 515 520 525 Ser Glu Glu Val Ala Ser Ile Glu Ser Leu Gly Gly Val Ile Ala Lys 530 535 540 Ser Leu Gln Thr Tyr Phe Ala Ala Glu Gly Ser Glu Ile Leu Leu Arg 545 550 555 560 Glu Leu Lys Glu Thr Gly Val Asn Leu Asp Tyr Lys Gly Gln Thr Val 565 570 575 Val Ala Asp Ala Ala Leu Ser Gly Leu Thr Val Val Leu Thr Gly Lys 580 585 590 Leu Glu Arg Leu Lys Arg Ser Glu Ala Lys Ser Lys Leu Glu Ser Leu 595 600 605 Gly Ala Lys Val Thr Gly Ser Val Ser Lys Lys Thr Asp Leu Val Val 610 615 620 Val Gly Ala Asp Ala Gly Ser Lys Leu Gln Lys Ala Gln Glu Leu Gly 625 630 635 640 Ile Gln Val Arg Asp Glu Ala Trp Leu Glu Ser Leu 645 650 <210> 105 <211> 1302 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene <222> (1) .. (1301) <223> Streptococcus pneumoniae spr1036 gene <400> 105 atgtcaatta ttactgatgt ttacgctcgc gaagtcctag actcacgcgg taacccaaca 60 cttgaagtag aagtttacac tgaatcaggt gctttcggac gtggtatggt tccatcagga 120 gcttctactg gtgaacacga agcagttgaa cttcgcgacg gtgacaaatc tcgttacggt 180 ggtcttggta cacaaaaagc tgttgacaac gtaaacaaca tcattgctga agctatcatt 240 ggctacgatg tacgtgatca acaagctatt gaccgtgcta tgatcgcact tgacggtact 300 cctaacaaag gtaaattggg tgcgaatgca atcctcggtg tgtctatcgc tgtagctcgt 360 gctgctgctg actaccttga aatcccactt tacagctacc ttggtggatt caacactaaa 420 gttcttccaa ctccaatgat gaacatcatc aacggtggtt ctcactctga cgctccaatc 480 gctttccaag agttcatgat cttgccagtt ggtgcgccaa catttaaaga agcccttcgt 540 tacggtgctg aaatcttcca cgctcttaag aaaatcctta aatcacgtgg tttggaaact 600 gccgtaggtg acgaaggtgg attcgctcct cgtttcgaag gaactgaaga tggtgttgaa 660 actatccttg ctgcgattga agctgctgga tatgtaccag gtaaagacgt atttctcgga 720 tttgactgtg cttcatcaga attctacgat aaagaacgta aagtttacga ctacactaaa 780 tttgaaggcg aaggtgctgc tgttcgtaca tctgcagaac aaatcgacta ccttgaagaa 840 ttggttaaca aatacccaat catcactatt gaagatggta tggatgaaaa cgactgggat 900 ggttggaaag ctcttactga acgtcttggt aagaaagtac aacttgttgg tgacgacttc 960 ttcgtaacaa acactgacta ccttgcacgt ggtatccaag aaggtgctgc taactcaatc 1020 cttatcaaag ttaaccaaat cggtactctt actgaaactt ttgaagctat cgaaatggct 1080 aaagaagctg gttacactgc tgttgtatca caccgttcag gtgaaactga agattcaaca 1140 atcgctgata tcgcagttgc aactaacgca ggacaaatca agactggttc actttcacgt 1200 acagaccgta tcgctaaata caaccaattg cttcgtatcg aagaccaact tggtgaagta 1260 gctgaatatc gtggattgaa atcattctac aaccttaaaa aa 1302 <210> 106 <211> 434 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (434) <223> Enolase <400> 106 Met Ser Ile Ile Thr Asp Val Tyr Ala Arg Glu Val Leu Asp Ser Arg 1 5 10 15 Gly Asn Pro Thr Leu Glu Val Glu Val Tyr Thr Glu Ser Gly Ala Phe 20 25 30 Gly Arg Gly Met Val Pro Ser Gly Ala Ser Thr Gly Glu His Glu Ala 35 40 45 Val Glu Leu Arg Asp Gly Asp Lys Ser Arg Tyr Gly Gly Leu Gly Thr 50 55 60 Gln Lys Ala Val Asp Asn Val Asn Asn Ile Ile Ala Glu Ala Ile Ile 65 70 75 80 Gly Tyr Asp Val Arg Asp Gln Gln Ala Ile Asp Arg Ala Met Ile Ala 85 90 95 Leu Asp Gly Thr Pro Asn Lys Gly Lys Leu Gly Ala Asn Ala Ile Leu 100 105 110 Gly Val Ser Ile Ala Val Ala Arg Ala Ala Ala Asp Tyr Leu Glu Ile 115 120 125 Pro Leu Tyr Ser Tyr Leu Gly Gly Phe Asn Thr Lys Val Leu Pro Thr 130 135 140 Pro Met Met Asn Ile Ile Asn Gly Gly Ser His Ser Asp Ala Pro Ile 145 150 155 160 Ala Phe Gln Glu Phe Met Ile Leu Pro Val Gly Ala Pro Thr Phe Lys 165 170 175 Glu Ala Leu Arg Tyr Gly Ala Glu Ile Phe His Ala Leu Lys Lys Ile 180 185 190 Leu Lys Ser Arg Gly Leu Glu Thr Ala Val Gly Asp Glu Gly Gly Phe 195 200 205 Ala Pro Arg Phe Glu Gly Thr Glu Asp Gly Val Glu Thr Ile Leu Ala 210 215 220 Ala Ile Glu Ala Ala Gly Tyr Val Pro Gly Lys Asp Val Phe Leu Gly 225 230 235 240 Phe Asp Cys Ala Ser Ser Glu Phe Tyr Asp Lys Glu Arg Lys Val Tyr 245 250 255 Asp Tyr Thr Lys Phe Glu Gly Glu Gly Ala Ala Val Arg Thr Ser Ala 260 265 270 Glu Gln Ile Asp Tyr Leu Glu Glu Leu Val Asn Lys Tyr Pro Ile Ile 275 280 285 Thr Ile Glu Asp Gly Met Asp Glu Asn Asp Trp Asp Gly Trp Lys Ala 290 295 300 Leu Thr Glu Arg Leu Gly Lys Lys Val Gln Leu Val Gly Asp Asp Phe 305 310 315 320 Phe Val Thr Asn Thr Asp Tyr Leu Ala Arg Gly Ile Gln Glu Gly Ala 325 330 335 Ala Asn Ser Ile Leu Ile Lys Val Asn Gln Ile Gly Thr Leu Thr Glu 340 345 350 Thr Phe Glu Ala Ile Glu Met Ala Lys Glu Ala Gly Tyr Thr Ala Val 355 360 365 Val Ser His Arg Ser Gly Glu Thr Glu Asp Ser Thr Ile Ala Asp Ile 370 375 380 Ala Val Ala Thr Asn Ala Gly Gln Ile Lys Thr Gly Ser Leu Ser Arg 385 390 395 400 Thr Asp Arg Ile Ala Lys Tyr Asn Gln Leu Leu Arg Ile Glu Asp Gln 405 410 415 Leu Gly Glu Val Ala Glu Tyr Arg Gly Leu Lys Ser Phe Tyr Asn Leu 420 425 430 Lys Lys <210> 107 <211> 261 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (261) <223> Streptococcus pneumoniae spr1063 gene <400> 107 atggcttcta aagatttcca cgtagtggca gaaacaggta ttcacgcacg tccagcaaca 60 ttgttggtac aaactgctag caaatttgct tcagatatca ctcttgagta caaaggtaaa 120 tcagttaacc ttaaatcaat tatgggtgtt atgagtcttg gtgttggcca aggtgctgac 180 gtaactatct cagctgaagg tgcagatgca gatgacgcta tcgctgcaat ctcagaaaca 240 atggaaaaag aaggattggc a 261 <210> 108 <211> 87 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (87) <223> Histidine-containing phosphocarrier protein <400> 108 Met Ala Ser Lys Asp Phe His Val Val Ala Glu Thr Gly Ile His Ala 1 5 10 15 Arg Pro Ala Thr Leu Leu Val Gln Thr Ala Ser Lys Phe Ala Ser Asp 20 25 30 Ile Thr Leu Glu Tyr Lys Gly Lys Ser Val Asn Leu Lys Ser Ile Met 35 40 45 Gly Val Met Ser Leu Gly Val Gly Gln Gly Ala Asp Val Thr Ile Ser 50 55 60 Ala Glu Gly Ala Asp Ala Asp Asp Ala Ile Ala Ala Ile Ser Glu Thr 65 70 75 80 Met Glu Lys Glu Gly Leu Ala 85 <210> 109 <211> 234 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (234) <223> Streptococcus pneumoniae spr1064 gene <400> 109 ttgaagagga gttacacaat ggtaaccgtt tattctaaaa acaattgtgt ccaatgtaaa 60 atgaccaagc gtttcttgga cagtaataat gtctcttatc gtgaaatcaa tcttgatgag 120 caacctgagt acgtcgatca agttaaagag ctcggtttta gcgcagctcc tgttatccaa 180 acaccaactg aagtcttttc aggtttccaa ccaggaaaac tgaaacaatt agca 234 <210> 110 <211> 78 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (78) <223> Glutaredoxin-like protein involved in electron transport system for ribonucleotide reductase system NrdEF <400> 110 Met Lys Arg Ser Tyr Thr Met Val Thr Val Tyr Ser Lys Asn Asn Cys 1 5 10 15 Val Gln Cys Lys Met Thr Lys Arg Phe Leu Asp Ser Asn Asn Val Ser 20 25 30 Tyr Arg Glu Ile Asn Leu Asp Glu Gln Pro Glu Tyr Val Asp Gln Val 35 40 45 Lys Glu Leu Gly Phe Ser Ala Ala Pro Val Ile Gln Thr Pro Thr Glu 50 55 60 Val Phe Ser Gly Phe Gln Pro Gly Lys Leu Lys Gln Leu Ala 65 70 75 <210> 111 <211> 2157 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (2157) <223> Streptococcus pneumoniae spr1065 gene <400> 111 atgggattaa aacatcttga agacgtgact tacttccgtc ttaataacga aattaaccgt 60 cctgttaatg gacaaatcat gcttcataaa gacaaggaag ccttggatgc tttctttaaa 120 gaaaatgtag ttccaaacac tatggttttt gattcaatca aagacaaaat caattacctc 180 attgaacaca actacatcga aacagccttt atcaagaaat accgtccaga gttcttggaa 240 gaattagctc aatttatcaa agaccaaaac ttccaattca agtcattcat ggcagcttat 300 aaattttaca atcaatatgc cttaaagact aatgacggtg aatactatct tgaaaatatg 360 gaagaccgtg tcttcttcaa cgccctttat ttcgctgatg ggaatgaagc tgttgcaatc 420 gatattgcca atgaaatcat ccaccaacgc taccaacctg ctactccttc cttcttgaat 480 gctggacgtg ctcgccgtgg agagttggta tcttgtttcc taatccaagt gacggatgat 540 atgaactcta tcggacgttc tatcaactca gctcttcaac tttcacgtat tggtggtggt 600 gtgggaatta ccctcagcaa ccttcgtgaa gctggtgcac ctatcaaagg ctatgaagga 660 gctgcttcag gtgtcgttcc tgttatgaag ctttttgaag acagcttctc ttactccaac 720 caattggggc aacgtcaagg tgccggtgtt gtctacctca acgtctttca cccagatatc 780 atcgctttcc tttcaactaa gaaagaaaac gctgatgaaa aagtacgtgt caagactcta 840 tcacttggtg ttgtagtacc tgataaattc tacgaattgg ctcgtaaaaa tgaagaaatg 900 tacctcttca gcccttactc tgtagaaaaa gaatacggtg tgccattcaa ttacatcgac 960 attactgaaa aatacgatga attggtcgca aatccaaata ttcgcaagac aaaaatcaag 1020 gcgcgggatt tggaaactga aatctctaaa ttgcaacaag agtctggcta tccttatgta 1080 gtcaacatcg atacggctaa ccgtgcaaat cctgttgatg gaaagattat catgagtaac 1140 ttgtgttctg agattcttca agttcaagaa ccaagcttga tcaacgatgc tcaagaattc 1200 cttcaaatgg gaacagatgt ttcatgtaac ctgggttcaa ccaacgtggt taacatgatg 1260 acttcacctg attttggtcg ttctatccgt gctatggttc gtgcccttac tttcgttaca 1320 gatagttcac atatcgtagc tgtaccaacg attgaccatg gaaatagcca agcccacacc 1380 tttggtctcg gtgccatggg actgcacagc taccttgccc aacaactcat tgaatatggt 1440 tcacctgagt ctgttgaatt tacaagcatc tactttatgc ttatgaacta ctggaccttg 1500 gtagagtcaa acaatattgc gcgtgaacgt ggtattacct tccacaactt tgaaaaatca 1560 gactatgcta acggaagtta ctttgacaag tatgtaactg gtgaatttgt tccaacatca 1620 gaccgtgtta aagaactctt caaaaatgtc tttatccctg gtgttgctga ttgggctgaa 1680 cttcgcgaca aggttcaaga agatggtctt taccaccaaa atcgccttgc tgtagcgcca 1740 aatggttcta tcagctatat caacgacgtt tctgcttcta tccacccgat tacgcaacgt 1800 atcgaagaac gccaagagaa gaaaattggt aaaatctact accctgctgc tggcttgtct 1860 acagaaacca ttccttacta cacttctgcc tacgatatgg atatgcgtaa agtcatcgat 1920 gtttacgctg ctgcgactga acacgtggat caaggacttt cactcaccct cttcatgcgt 1980 agtgacattc caaaaggcct ttacgaatgg aagagggaaa ataaacaaac gacacgtgac 2040 ttgtccatcc ttcgtaacta tgcctttaac aagggaatca agtctatcta ctacgtccgt 2100 acctttacag atgacggtgg agaagtcggc gccaaccaat gtgaaagctg tgtgatt 2157 <210> 112 <211> 719 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (719) <223> Ribonucleoside-diphosphate reductase <400> 112 Met Gly Leu Lys His Leu Glu Asp Val Thr Tyr Phe Arg Leu Asn Asn 1 5 10 15 Glu Ile Asn Arg Pro Val Asn Gly Gln Ile Met Leu His Lys Asp Lys 20 25 30 Glu Ala Leu Asp Ala Phe Phe Lys Glu Asn Val Val Pro Asn Thr Met 35 40 45 Val Phe Asp Ser Ile Lys Asp Lys Ile Asn Tyr Leu Ile Glu His Asn 50 55 60 Tyr Ile Glu Thr Ala Phe Ile Lys Lys Tyr Arg Pro Glu Phe Leu Glu 65 70 75 80 Glu Leu Ala Gln Phe Ile Lys Asp Gln Asn Phe Gln Phe Lys Ser Phe 85 90 95 Met Ala Ala Tyr Lys Phe Tyr Asn Gln Tyr Ala Leu Lys Thr Asn Asp 100 105 110 Gly Glu Tyr Tyr Leu Glu Asn Met Glu Asp Arg Val Phe Phe Asn Ala 115 120 125 Leu Tyr Phe Ala Asp Gly Asn Glu Ala Val Ala Ile Asp Ile Ala Asn 130 135 140 Glu Ile Ile His Gln Arg Tyr Gln Pro Ala Thr Pro Ser Phe Leu Asn 145 150 155 160 Ala Gly Arg Ala Arg Arg Gly Glu Leu Val Ser Cys Phe Leu Ile Gln 165 170 175 Val Thr Asp Asp Met Asn Ser Ile Gly Arg Ser Ile Asn Ser Ala Leu 180 185 190 Gln Leu Ser Arg Ile Gly Gly Gly Val Gly Ile Thr Leu Ser Asn Leu 195 200 205 Arg Glu Ala Gly Ala Pro Ile Lys Gly Tyr Glu Gly Ala Ala Ser Gly 210 215 220 Val Val Pro Val Met Lys Leu Phe Glu Asp Ser Phe Ser Tyr Ser Asn 225 230 235 240 Gln Leu Gly Gln Arg Gln Gly Ala Gly Val Val Tyr Leu Asn Val Phe 245 250 255 His Pro Asp Ile Ile Ala Phe Leu Ser Thr Lys Lys Glu Asn Ala Asp 260 265 270 Glu Lys Val Arg Val Lys Thr Leu Ser Leu Gly Val Val Val Pro Asp 275 280 285 Lys Phe Tyr Glu Leu Ala Arg Lys Asn Glu Glu Met Tyr Leu Phe Ser 290 295 300 Pro Tyr Ser Val Glu Lys Glu Tyr Gly Val Pro Phe Asn Tyr Ile Asp 305 310 315 320 Ile Thr Glu Lys Tyr Asp Glu Leu Val Ala Asn Pro Asn Ile Arg Lys 325 330 335 Thr Lys Ile Lys Ala Arg Asp Leu Glu Thr Glu Ile Ser Lys Leu Gln 340 345 350 Gln Glu Ser Gly Tyr Pro Tyr Val Val Asn Ile Asp Thr Ala Asn Arg 355 360 365 Ala Asn Pro Val Asp Gly Lys Ile Ile Met Ser Asn Leu Cys Ser Glu 370 375 380 Ile Leu Gln Val Gln Glu Pro Ser Leu Ile Asn Asp Ala Gln Glu Phe 385 390 395 400 Leu Gln Met Gly Thr Asp Val Ser Cys Asn Leu Gly Ser Thr Asn Val 405 410 415 Val Asn Met Met Thr Ser Pro Asp Phe Gly Arg Ser Ile Arg Ala Met 420 425 430 Val Arg Ala Leu Thr Phe Val Thr Asp Ser Ser His Ile Val Ala Val 435 440 445 Pro Thr Ile Asp His Gly Asn Ser Gln Ala His Thr Phe Gly Leu Gly 450 455 460 Ala Met Gly Leu His Ser Tyr Leu Ala Gln Gln Leu Ile Glu Tyr Gly 465 470 475 480 Ser Pro Glu Ser Val Glu Phe Thr Ser Ile Tyr Phe Met Leu Met Asn 485 490 495 Tyr Trp Thr Leu Val Glu Ser Asn Asn Ila Ala Arg Glu Arg Gly Ile 500 505 510 Thr Phe His Asn Phe Glu Lys Ser Asp Tyr Ala Asn Gly Ser Tyr Phe 515 520 525 Asp Lys Tyr Val Thr Gly Glu Phe Val Pro Thr Ser Asp Arg Val Lys 530 535 540 Glu Leu Phe Lys Asn Val Phe Ile Pro Gly Val Ala Asp Trp Ala Glu 545 550 555 560 Leu Arg Asp Lys Val Gln Glu Asp Gly Leu Tyr His Gln Asn Arg Leu 565 570 575 Ala Val Ala Pro Asn Gly Ser Ile Ser Tyr Ile Asn Asp Val Ser Ala 580 585 590 Ser Ile His Pro Ile Thr Gln Arg Ile Glu Glu Arg Gln Glu Lys Lys 595 600 605 Ile Gly Lys Ile Tyr Tyr Pro Ala Ala Gly Leu Ser Thr Glu Thr Ile 610 615 620 Pro Tyr Tyr Thr Ser Ala Tyr Asp Met Asp Met Arg Lys Val Ile Asp 625 630 635 640 Val Tyr Ala Ala Ala Thr Glu His Val Asp Gln Gly Leu Ser Leu Thr 645 650 655 Leu Phe Met Arg Ser Asp Ile Pro Lys Gly Leu Tyr Glu Trp Lys Arg 660 665 670 Glu Asn Lys Gln Thr Thr Arg Asp Leu Ser Ile Leu Arg Asn Tyr Ala 675 680 685 Phe Asn Lys Gly Ile Lys Ser Ile Tyr Tyr Val Arg Thr Phe Thr Asp 690 695 700 Asp Gly Gly Glu Val Gly Ala Asn Gln Cys Glu Ser Cys Val Ile 705 710 715 <210> 113 <211> 270 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (270) <223> Streptococcus pneumoniae spr1081 gene <400> 113 atgattcctc acacagatat tgttcataat ctagctgaga aagtggtggt tgttcgatta 60 gagaaaccgg tgactttcca taatatgatt gcgccaggta aggaagtaga agtatccttg 120 ctcttcttta tcatcaataa ttcaagttca agtcaaacga acattcttgc tcagttgatg 180 gactttttca caggaaatgg acatcttgaa gacctatcaa aaatttccga accagaaaaa 240 ctttatgctt atatagatga agcaatcgct 270 <210> 114 <211> 90 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (90) <223> Conserved hypothetical protein <400> 114 Met Ile Pro His Thr Asp Ile Val His Asn Leu Ala Glu Lys Val Val 1 5 10 15 Val Val Arg Leu Glu Lys Pro Val Thr Phe His Asn Met Ile Ala Pro 20 25 30 Gly Lys Glu Val Glu Val Ser Leu Leu Phe Phe Ile Ile Asn Asn Ser 35 40 45 Ser Ser Ser Gln Thr Asn Ile Leu Ala Gln Leu Met Asp Phe Phe Thr 50 55 60 Gly Asn Gly His Leu Glu Asp Leu Ser Lys Ile Ser Glu Pro Glu Lys 65 70 75 80 Leu Tyr Ala Tyr Ile Asp Glu Ala Ile Ala 85 90 <210> 115 <211> 549 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (549) <223> Streptococcus pneumoniae spr1111 gene <400> 115 atggcaaaca ttctcttggc tgtaacgggt tcaatcgcct cttataagtc ggcagattta 60 gtcagttctc taaaaaaaca aggccatcaa gtcactgtct taatgactca ggctgctaca 120 gagtttatcc aacctttgac actacaggta ctctcacaga atcctgtcca cttggatgtc 180 atgaaggaac cctatcctga tcaggtcaat catatcgaac ttggaaaaaa agcagattta 240 tttatcgtgg tacctgcaac tgctaacact attgcaaaac tagctcacgg atttgcggac 300 aacatggtaa ccagtacagc tctagcccta ccaagtcata ttcccaaact aatagctcct 360 gctatgaata caaaaatgta tgaccatcca gtaactcaga ataatctgaa aacattagaa 420 acctacggct atcagctgat tgctcctaag gaatccctac tagcttgtgg agaccacgga 480 cgaggagctt tagctgacct cacaattatt ttagaaagaa taaaggaaac tatcgatgaa 540 aaaacgctc 549 <210> 116 <211> 183 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (183) <223> Similar to N-terminal region of the Dfp protein <400> 116 Met Ala Asn Ile Leu Leu Ala Val Thr Gly Ser Ile Ala Ser Tyr Lys 1 5 10 15 Ser Ala Asp Leu Val Ser Ser Leu Lys Lys Gln Gly His Gln Val Thr 20 25 30 Val Leu Met Thr Gln Ala Ala Thr Glu Phe Ile Gln Pro Leu Thr Leu 35 40 45 Gln Val Leu Ser Gln Asn Pro Val His Leu Asp Val Met Lys Glu Pro 50 55 60 Tyr Pro Asp Gln Val Asn His Ile Glu Leu Gly Lys Lys Ala Asp Leu 65 70 75 80 Phe Ile Val Val Pro Ala Thr Ala Asn Thr Ile Ala Lys Leu Ala His 85 90 95 Gly Phe Ala Asp Asn Met Val Thr Ser Thr Ala Leu Ala Leu Pro Ser 100 105 110 His Ile Pro Lys Leu Ile Ala Pro Ala Met Asn Thr Lys Met Tyr Asp 115 120 125 His Pro Val Thr Gln Asn Asn Leu Lys Thr Leu Glu Thr Tyr Gly Tyr 130 135 140 Gln Leu Ile Ala Pro Lys Glu Ser Leu Leu Ala Cys Gly Asp His Gly 145 150 155 160 Arg Gly Ala Leu Ala Asp Leu Thr Ile Ile Leu Glu Arg Ile Lys Glu 165 170 175 Thr Ile Asp Glu Lys Thr Leu 180 <210> 117 <211> 1485 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1485) <223> Streptococcus pneumoniae spr1150 gene <400> 117 atgaaaagta taaaattaaa tgctctatct tacatgggaa ttcgtgtctt gaatattatt 60 tttcccatcc taactggaac ctatgtcgcg cgtgtcttgg accgaactga ctatggttac 120 ttcaactcag tcgacactat tttgtcattt ttcttgccct ttgcaactta tggtgtctat 180 aactacggtt taagggctat cagtaatgtc aaggataaca aaaaagatct taacagaacc 240 ttttctagtc ttttttattt gtgcatcgct tgtacgattt tgaccactgc tgtctatatc 300 ctagcctatc ctctcttctt tactgataat ccaatcgtca aaaaggtcta ccttgttatg 360 gggattcaac tcattgccca gattttttca atcgaatggg tcaatgaagc tctggaaaat 420 tacagttttc tcttttacaa aactgccttc atccgtatcc tgatgctggt ctctattttc 480 ttatttgtta aaaatgaaca cgatattgtt gtctatacac ttgtgatgag tttatcgacg 540 ctgattaact acctgattag ttatttttgg attaaaagag acatcaaact tgttaaaatt 600 cacctaagtg attttaaacc actctttctc cctctgacag ccatgttagt ctttgccaat 660 gccaatatgc tcttcacttt tttagatcgc ctcttcctcg ttaaaacagg gattgatgtc 720 aacgttagtt actataccat agctcagcga attgtgaccg ttatagctgg ggttgtaaca 780 ggtgcaattg gagtgagtgt gcctcgtctc agttactatc tggggaaagg agacaaagaa 840 gcctatgttt ctctggttaa tagaggtagt cgaatcttta acttctttat cattccactg 900 agttttggac tcatggtttt aggaccaaat gccatcctac tttacggtag tgaaaaatat 960 atcggaggcg gcatcttgac ctctctcttc gcttttcgta cgattatcct ggccttagat 1020 accattcttg gttcccaaat tctctttacc aatggctatg aaaaacgtat cacagtctat 1080 acagtctttg ctgggctact caatttgggc ttgaatagtc tccttttttt caaccatatc 1140 gtggctcctg aatactactt actgacaact atgctatcag agacttctct acttgttttc 1200 tatatcattt tcatccatag aaaacaactc atccacttgg gacatatctt tagctatact 1260 gttcgatact ctctcttttc actttccttt gtagcaattt atttcctgat taatttcgtg 1320 tatcctgtag atatggtcat taatttgcca tttttgatta atactggttt gattgtcttg 1380 ctatcagcta tctcttatat tagtctactt gtcttcacaa aagatagcat tttctatgaa 1440 tttttaaacc atgtcctagc cttaaaaaat aaatttaaaa aatca 1485 <210> 118 <211> 495 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (495) <223> Conserved hypothetical protein <400> 118 Met Lys Ser Ile Lys Leu Asn Ala Leu Ser Tyr Met Gly Ile Arg Val 1 5 10 15 Leu Asn Ile Ile Phe Pro Ile Leu Thr Gly Thr Tyr Val Ala Arg Val 20 25 30 Leu Asp Arg Thr Asp Tyr Gly Tyr Phe Asn Ser Val Asp Thr Ile Leu 35 40 45 Ser Phe Phe Leu Pro Phe Ala Thr Tyr Gly Val Tyr Asn Tyr Gly Leu 50 55 60 Arg Ala Ile Ser Asn Val Lys Asp Asn Lys Lys Asp Leu Asn Arg Thr 65 70 75 80 Phe Ser Ser Leu Phe Tyr Leu Cys Ile Ala Cys Thr Ile Leu Thr Thr 85 90 95 Ala Val Tyr Ile Leu Ala Tyr Pro Leu Phe Phe Thr Asp Asn Pro Ile 100 105 110 Val Lys Lys Val Tyr Leu Val Met Gly Ile Gln Leu Ile Ala Gln Ile 115 120 125 Phe Ser Ile Glu Trp Val Asn Glu Ala Leu Glu Asn Tyr Ser Phe Leu 130 135 140 Phe Tyr Lys Thr Ala Phe Ile Arg Ile Leu Met Leu Val Ser Ile Phe 145 150 155 160 Leu Phe Val Lys Asn Glu His Asp Ile Val Val Tyr Thr Leu Val Met 165 170 175 Ser Leu Ser Thr Leu Ile Asn Tyr Leu Ile Ser Tyr Phe Trp Ile Lys 180 185 190 Arg Asp Ile Lys Leu Val Lys Ile His Leu Ser Asp Phe Lys Pro Leu 195 200 205 Phe Leu Pro Leu Thr Ala Met Leu Val Phe Ala Asn Ala Asn Met Leu 210 215 220 Phe Thr Phe Leu Asp Arg Leu Phe Leu Val Lys Thr Gly Ile Asp Val 225 230 235 240 Asn Val Ser Tyr Tyr Thr Ile Ala Gln Arg Ile Val Thr Val Ile Ala 245 250 255 Gly Val Val Thr Gly Ala Ile Gly Val Ser Val Pro Arg Leu Ser Tyr 260 265 270 Tyr Leu Gly Lys Gly Asp Lys Glu Ala Tyr Val Ser Leu Val Asn Arg 275 280 285 Gly Ser Arg Ile Phe Asn Phe Phe Ile Ile Pro Leu Ser Phe Gly Leu 290 295 300 Met Val Leu Gly Pro Asn Ala Ile Leu Leu Tyr Gly Ser Glu Lys Tyr 305 310 315 320 Ile Gly Gly Gly Ile Leu Thr Ser Leu Phe Ala Phe Arg Thr Ile Ile 325 330 335 Leu Ala Leu Asp Thr Ile Leu Gly Ser Gln Ile Leu Phe Thr Asn Gly 340 345 350 Tyr Glu Lys Arg Ile Thr Val Tyr Thr Val Phe Ala Gly Leu Leu Asn 355 360 365 Leu Gly Leu Asn Ser Leu Leu Phe Phe Asn His Ile Val Ala Pro Glu 370 375 380 Tyr Tyr Leu Leu Thr Thr Met Metu Ser Glu Thr Ser Leu Leu Val Phe 385 390 395 400 Tyr Ile Ile Phe Ile His Arg Lys Gln Leu Ile His Leu Gly His Ile 405 410 415 Phe Ser Tyr Thr Val Arg Tyr Ser Leu Phe Ser Leu Ser Phe Val Ala 420 425 430 Ile Tyr Phe Leu Ile Asn Phe Val Tyr Pro Val Asp Met Val Ile Asn 435 440 445 Leu Pro Phe Leu Ile Asn Thr Gly Leu Ile Val Leu Leu Ser Ala Ile 450 455 460 Ser Tyr Ile Ser Leu Leu Val Phe Thr Lys Asp Ser Ile Phe Tyr Glu 465 470 475 480 Phe Leu Asn His Val Leu Ala Leu Lys Asn Lys Phe Lys Lys Ser 485 490 495 <210> 119 <211> 540 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (540) <223> Streptococcus pneumoniae spr1158 gene <400> 119 atgaaattaa atattcaaga aattcgtaag cagtctgaag gtttgaactt tgaacaaacg 60 ttagatttag ttgatgacct gcgtgcacgt aatcaagaaa ttttagatgt aaaagatatc 120 cttgcagttg ggaaagtaca atatgaagac cgtatgtatt tcttagatta tcaactatct 180 tataccattg ttcttgcttc gagtcgcagt atggagccag ttgagttagt tgaatcttat 240 ccagtcacgg aagttttcat ggaaggcgca actaaccagc tagatcaaga agttttagat 300 gatgacttgg tcttgcccat cgaaaatggg gagcttgacc ttgctgagag tgtatcagac 360 aatatcctgc taaacattcc tatcaaggtc ttgacggctg aagaagaagc tggtcaagga 420 tttatctcag gaaatgactg gcaaatcatg acagaggaag aataccaagc tcaaaaagca 480 gtaaagaaag aagaaaacag tccttttgct ggcttacaag gactatttga cggagatgaa 540 540 <210> 120 <211> 180 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (180) <223> Conserved hypothetical protein <400> 120 Met Lys Leu Asn Ile Gln Glu Ile Arg Lys Gln Ser Glu Gly Leu Asn 1 5 10 15 Phe Glu Gln Thr Leu Asp Leu Val Asp Asp Leu Arg Ala Arg Asn Gln 20 25 30 Glu Ile Leu Asp Val Lys Asp Ile Leu Ala Val Gly Lys Val Gln Tyr 35 40 45 Glu Asp Arg Met Tyr Phe Leu Asp Tyr Gln Leu Ser Tyr Thr Ile Val 50 55 60 Leu Ala Ser Ser Arg Ser Met Glu Pro Val Glu Leu Val Glu Ser Tyr 65 70 75 80 Pro Val Thr Glu Val Phe Met Glu Gly Ala Thr Asn Gln Leu Asp Gln 85 90 95 Glu Val Leu Asp Asp Asp Leu Val Leu Pro Ile Glu Asn Gly Glu Leu 100 105 110 Asp Leu Ala Glu Ser Val Ser Asp Asn Ile Leu Leu Asn Ile Pro Ile 115 120 125 Lys Val Leu Thr Ala Glu Glu Glu Ala Gly Gln Gly Phe Ile Ser Gly 130 135 140 Asn Asp Trp Gln Ile Met Thr Glu Glu Glu Tyr Gln Ala Gln Lys Ala 145 150 155 160 Val Lys Lys Glu Glu Asn Ser Pro Phe Ala Gly Leu Gln Gly Leu Phe 165 170 175 Asp Gly Asp Glu 180 <210> 121 <211> 723 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (723) <223> Streptococcus pneumoniae spr1282 gene <400> 121 atgcgtgatg ccaaccgtgg tggatgttct cagtcatgcc gttggaagta cgacctttac 60 gatatgccat ttgggaaaga acgtaagagt ttgcagggtg agattccaga agaattttca 120 atgtcagctg ttgatatgtc tatgattgac catatctcag atatgattga aaatggtgtg 180 gacagtctaa aaatcgaagg acgtatggag tctattcact atgtattaac agtaaccaac 240 tgctacaagg cggctgtgga tgcctatctt gaaagtcctg aaaagtttga agctatcaaa 300 caagacttgg tggacgagat gtggaaggtt gcccaacgtg aactggctac aggattttac 360 tatggtacac catctgaaaa tgagcagttg tttggtgctc gtcgtaaaat ccctgagtac 420 aagtttgtcg ctgaagtggt ttcttatgat gatgcggcac aaacagcaac tattcgtcaa 480 cgaaacgtca ttaacgaagg ggaccaagtt gagttttatg gtccaggttt ccgtcatttt 540 gaaacctata ttgaagattt gcatgatgcc aaaggcaata aaatcgaccg cgctccaaat 600 ccaatggaac tattgactat taaggtgcct caacctgttc aagctggaga catggttcgt 660 gcattaaaag aaggactcat caatctttat aaggaagatg gaaccagcgt cacagttcgt 720 gct 723 <210> 122 <211> 241 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (241) <223> Conserved hypothetical protein <400> 122 Met Arg Asp Ala Asn Arg Gly Gly Cys Ser Gln Ser Cys Arg Trp Lys 1 5 10 15 Tyr Asp Leu Tyr Asp Met Pro Phe Gly Lys Glu Arg Lys Ser Leu Gln 20 25 30 Gly Glu Ile Pro Glu Glu Phe Ser Met Ser Ala Val Asp Met Ser Met 35 40 45 Ile Asp His Ile Ser Asp Met Ile Glu Asn Gly Val Asp Ser Leu Lys 50 55 60 Ile Glu Gly Arg Met Glu Ser Ile His Tyr Val Leu Thr Val Thr Asn 65 70 75 80 Cys Tyr Lys Ala Ala Val Asp Ala Tyr Leu Glu Ser Pro Glu Lys Phe 85 90 95 Glu Ala Ile Lys Gln Asp Leu Val Asp Glu Met Trp Lys Val Ala Gln 100 105 110 Arg Glu Leu Ala Thr Gly Phe Tyr Tyr Gly Thr Pro Ser Glu Asn Glu 115 120 125 Gln Leu Phe Gly Ala Arg Arg Lys Ile Pro Glu Tyr Lys Phe Val Ala 130 135 140 Glu Val Val Ser Tyr Asp Asp Ala Ala Gln Thr Ala Thr Ile Arg Gln 145 150 155 160 Arg Asn Val Ile Asn Glu Gly Asp Gln Val Glu Phe Tyr Gly Pro Gly 165 170 175 Phe Arg His Phe Glu Thr Tyr Ile Glu Asp Leu His Asp Ala Lys Gly 180 185 190 Asn Lys Ile Asp Arg Ala Pro Asn Pro Met Glu Leu Leu Thr Ile Lys 195 200 205 Val Pro Gln Pro Val Gln Ala Gly Asp Met Val Arg Ala Leu Lys Glu 210 215 220 Gly Leu Ile Asn Leu Tyr Lys Glu Asp Gly Thr Ser Val Thr Val Arg 225 230 235 240 Ala <210> 123 <211> 909 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (909) <223> Streptococcus pneumoniae spr1312 gene <400> 123 atgtacgata ctattattat cggtgctgga cctgcaggga tgactgcggc cttgtatgct 60 gctcgaagca atctaaaagt agctttgatt gaaggtggtc tgccaggtgg tcagatgaat 120 aatacatctg atatcgaaaa ttacccagga tacgctaata ttagtggacc tgaattggct 180 gaaaagatgt ttgaaccact tgaaaatctt ggtgttgagc acatttatgg ttatgttgaa 240 aatgtcgaag accatggtga ttttaagaaa gtgatgactg atgaccaaac atatgaaaca 300 cgtacagtta tcgtagcaac tggttctaaa caccgtcctt tgggagtacc tggagaagaa 360 gaactgaaca gtcgtggtgt ttcttactgt gctgtgtgtg atggtgcttt cttccgtgac 420 caagatttat tggtagttgg tggtggagat tcagctgttg aagaagccct cttcttgact 480 cgttttgcta agactgttac cattgttcac cgtcgtgacc aacttcgtgc ccaaaaggtt 540 ttacaagatc gcgcctttgc gaatgaaaaa atcagcttta tctgggattc tgtagtgaag 600 gaaatcaagg gtgaaaacag agtagaatct gtcgtttttg aaaatgtgaa aacaggtcaa 660 gtgacagagc aagccttcgg tggtgtcttt atctatgttg gtttggaccc tcttagcgat 720 tttgttaaag aattgaatat ccaagatcag gcaggctgga ttgtgacaga tagccacatg 780 aagactgcag tcgacggtat ctttgcagtt ggagatgttc gcttgaaaga ccttcgccaa 840 gtaacaacag cagttggaga tggagctatc gctggtcaag aagcctacaa gtttatcaca 900 gaacatagt 909 <210> 124 <211> 303 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (303) <223> Thioredoxin reductase <400> 124 Met Tyr Asp Thr Ile Ile Gly Ala Gly Pro Ala Gly Met Thr Ala 1 5 10 15 Ala Leu Tyr Ala Ala Arg Ser Asn Leu Lys Val Ala Leu Ile Glu Gly 20 25 30 Gly Leu Pro Gly Gly Gln Met Asn Asn Thr Ser Asp Ile Glu Asn Tyr 35 40 45 Pro Gly Tyr Ala Asn Ile Ser Gly Pro Glu Leu Ala Glu Lys Met Phe 50 55 60 Glu Pro Leu Glu Asn Leu Gly Val Glu His Ile Tyr Gly Tyr Val Glu 65 70 75 80 Asn Val Glu Asp His Gly Asp Phe Lys Lys Val Met Thr Asp Asp Gln 85 90 95 Thr Tyr Glu Thr Arg Thr Val Ile Val Ala Thr Gly Ser Lys His Arg 100 105 110 Pro Leu Gly Val Pro Gly Glu Glu Glu Leu Asn Ser Arg Gly Val Ser 115 120 125 Tyr Cys Ala Val Cys Asp Gly Ala Phe Phe Arg Asp Gln Asp Leu Leu 130 135 140 Val Val Gly Gly Gly Asp Ser Ala Val Glu Glu Ala Leu Phe Leu Thr 145 150 155 160 Arg Phe Ala Lys Thr Val Thr Ile Val His Arg Arg Asp Gln Leu Arg 165 170 175 Ala Gln Lys Val Leu Gln Asp Arg Ala Phe Ala Asn Glu Lys Ile Ser 180 185 190 Phe Ile Trp Asp Ser Val Val Lys Glu Ile Lys Gly Glu Asn Arg Val 195 200 205 Glu Ser Val Val Phe Glu Asn Val Lys Thr Gly Gln Val Thr Glu Gln 210 215 220 Ala Phe Gly Gly Val Phe Ile Tyr Val Gly Leu Asp Pro Leu Ser Asp 225 230 235 240 Phe Val Lys Glu Leu Asn Ile Gln Asp Gln Ala Gly Trp Ile Val Thr 245 250 255 Asp Ser His Met Lys Thr Ala Val Asp Gly Ile Phe Ala Val Gly Asp 260 265 270 Val Arg Leu Lys Asp Leu Arg Gln Val Thr Thr Ala Val Gly Asp Gly 275 280 285 Ala Ile Ala Gly Gln Glu Ala Tyr Lys Phe Ile Thr Glu His Ser 290 295 300 <210> 125 <211> 915 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (915) <223> Streptococcus pneumoniae spr1329 gene <400> 125 atgtctaaga aattgacttt ccaggaaatc atccttactt tgcaacaatt ttggaatgac 60 caaggttgta tgcttatgca ggcttatgat aatgaaaaag gtgcgggaac aatgagtcct 120 tacactttcc ttcgtgctat cggacctgag ccatggaatg cagcttatgt agagccatca 180 cgtcgtcctg ctgacggtcg ttatggggaa aaccctaacc gtctctacca acaccaccaa 240 ttccaggtgg tcatgaagcc ttctccatca aatatccaag aactttacct tgagtctttg 300 gaaaaattgg gaatcaatcc tttggagcac gatattcgtt ttgttgagga caactgggaa 360 aacccatcaa ctggttcagc tggtcttggt tgggaagttt ggcttgacgg aatggaaatc 420 actcagttca cttatttcca acaagtcggt ggattggcaa ctggccctgt gactgcggaa 480 gttacctatg gtttggagcg cttggcttct tacattcaag aagtagactc tatctatgat 540 atcgagtggg ctgatggtgt aaaatacgga gaaatcttta tccagcctga gtatgagcac 600 tcaaaatatt catttgaaat ttcggaccaa gaaatgttgc ttgaaaactt tgataagttt 660 gaaaaagaag ctggtcgtgc attagaagaa ggcttggtac accctgccta tgactatgtt 720 ctcaaatgtt cacatacctt taatctgctt gacgcgcgtg gtgccgtatc tgtaacagag 780 cgtgcaggct atatcgctcg tatccgtaac ttggcccgtg ttgtagccaa aacctttgtt 840 gcagaacgca aacgcctagg ttacccactt ttggatgaag aaacacgagt taaactttta 900 gcagaagacg cagaa 915 <210> 126 <211> 305 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (305) <223> Glycyl-tRNA synthetase alpha chain <400> 126 Met Ser Lys Lys Leu Thr Phe Gln Glu Ile Ile Leu Thr Leu Gln Gln 1 5 10 15 Phe Trp Asn Asp Gln Gly Cys Met Leu Met Gln Ala Tyr Asp Asn Glu 20 25 30 Lys Gly Ala Gly Thr Met Ser Pro Tyr Thr Phe Leu Arg Ala Ile Gly 35 40 45 Pro Glu Pro Trp Asn Ala Ala Tyr Val Glu Pro Ser Arg Arg Pro Ala 50 55 60 Asp Gly Arg Tyr Gly Glu Asn Pro Asn Arg Leu Tyr Gln His His Gln 65 70 75 80 Phe Gln Val Val Met Lys Pro Ser Pro Ser Asn Ile Gln Glu Leu Tyr 85 90 95 Leu Glu Ser Leu Glu Lys Leu Gly Ile Asn Pro Leu Glu His Asp Ile 100 105 110 Arg Phe Val Glu Asp Asn Trp Glu Asn Pro Ser Thr Gly Ser Ala Gly 115 120 125 Leu Gly Trp Glu Val Trp Leu Asp Gly Met Glu Ile Thr Gln Phe Thr 130 135 140 Tyr Phe Gln Gln Val Gly Gly Leu Ala Thr Gly Pro Val Thr Ala Glu 145 150 155 160 Val Thr Tyr Gly Leu Glu Arg Leu Ala Ser Tyr Ile Gln Glu Val Asp 165 170 175 Ser Ile Tyr Asp Ile Glu Trp Ala Asp Gly Val Lys Tyr Gly Glu Ile 180 185 190 Phe Ile Gln Pro Glu Tyr Glu His Ser Lys Tyr Ser Phe Glu Ile Ser 195 200 205 Asp Gln Glu Met Leu Leu Glu Asn Phe Asp Lys Phe Glu Lys Glu Ala 210 215 220 Gly Arg Ala Leu Glu Glu Gly Leu Val His Pro Ala Tyr Asp Tyr Val 225 230 235 240 Leu Lys Cys Ser His Thr Phe Asn Leu Leu Asp Ala Arg Gly Ala Val 245 250 255 Ser Val Thr Glu Arg Ala Gly Tyr Ile Ala Arg Ile Arg Asn Leu Ala 260 265 270 Arg Val Val Ala Lys Thr Phe Val Ala Glu Arg Lys Arg Leu Gly Tyr 275 280 285 Pro Leu Leu Asp Glu Glu Thr Arg Val Lys Leu Leu Ala Glu Asp Ala 290 295 300 Glu 305 <210> 127 <211> 417 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (417) <223> Streptococcus pneumoniae spr1359 gene <400> 127 atggctcagt taactgtcca gatcgtgaca ccagatggtc tcgtctatga tcaccatgcc 60 agctatgtat cggttcgaac tctggatggt gagatgggga tcttgccacg acatgaaaat 120 atgattgcgg ttttagcagt tgatgaagta aaggtaaaac gtatcgatga taaagatcac 180 gtgaactgga ttgcagtaaa cggtggcgtt attgaaattg ccaatgatat gatcacaatc 240 gtcgctgact ctgcagaacg tgctcgtgat atcgatatca gtcgtgcaga acgtgccaaa 300 cttcgtgcag aacgtgcaat tgaagaagca caagacaaac atttgattga ccaagaacgt 360 cgtgctaaga ttgctttgca acgtgctatt aaccgtatta atgtcggaaa tagacta 417 <210> 128 <211> 139 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (139) <223> Proton-translocating ATPase, F1 sector, epsilon-subunit <400> 128 Met Ala Gln Leu Thr Val Gln Ile Val Thr Pro Asp Gly Leu Val Tyr 1 5 10 15 Asp His His Ala Ser Tyr Val Ser Val Arg Thr Leu Asp Gly Glu Met 20 25 30 Gly Ile Leu Pro Arg His Glu Asn Met Ile Ala Val Leu Ala Val Asp 35 40 45 Glu Val Lys Val Lys Arg Ile Asp Asp Lys Asp His Val Asn Trp Ile 50 55 60 Ala Val Asn Gly Gly Val Ile Glu Ile Ala Asn Asp Met Ile Thr Ile 65 70 75 80 Val Ala Asp Ser Ala Glu Arg Ala Arg Asp Ile Asp Ile Ser Arg Ala 85 90 95 Glu Arg Ala Lys Leu Arg Ala Glu Arg Ala Ile Glu Glu Ala Gln Asp 100 105 110 Lys His Leu Ile Asp Gln Glu Arg Arg Ala Lys I 115 120 125 Ala Ile Asn Arg Ile Asn Val Gly Asn Arg Leu 130 135 <210> 129 <211> 1404 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (1) .. (1404) <223> Streptococcus pneumoniae spr1360 gene <400> 129 atgagttcag gtaaaattgc tcaggttatc ggtcccgttg tagacgtttt gtttgcagca 60 ggggaaaaac ttcctgagat taacaatgca cttgtcgtct acaaaaatga cgaaagaaaa 120 acaaaaatcg tccttgaagt agccttggag ttaggagatg gtatggttcg tactatcgcc 180 atggaatcaa cagatgggtt gactcgtgga atggaagtat tggacacagg tcgtccaatc 240 tctgtaccag taggtaaaga aactttggga cgtgtcttca acgttttggg agataccatt 300 gacttggaag ctccttttac agaagacgca gagcgtcagc caattcataa aaaagctcca 360 acttttgatg agttgtctac ctcttctgaa atccttgaaa cagggatcaa ggttattgac 420 cttcttgccc cttaccttaa aggtggtaaa gttggacttt tcggtggtgc cggagttggt 480 aaaactgtct taatccaaga attgattcac aacattgccc aagagcacgg tggtatttca 540 gtatttgctg gtgttgggga acgtactcgt gaggggaatg acctttactg ggaaatgaaa 600 gaatcaggcg ttatcgagaa aacagccatg gtctttggtc agatgaatga gccaccagga 660 gcacgtatgc gtgttgccct tactggtttg acaatcgctg aatacttccg tgatgtggaa 720 ggccaagacg tgcttctctt tatcgataat atcttccgtt tcactcaggc tggttcagaa 780 gtatctgccc ttttgggtcg tatgccatca gccgttggtt accaaccaac acttgctacg 840 gaaatgggtc aattgcaaga acgtatcaca tcaaccaaga agggttctgt aacctctatc 900 caggctatct atgtgccagc ggatgactat actgacccag cgccagcaac agccttcgct 960 cacttggatt caacaacaaa cttggaacgt aagttggtac aattgggtat ctacccagcc 1020 gttgacccac ttgcttcaag ctcacgtgcc ttggcacctg aaatcgttgg agaagagcac 1080 tatgcagttg ctgctgaagt aaaacgtgtc cttcaacgtt accatgaatt gcaagatatc 1140 attgctatcc ttggtatgga tgagctttct gatgaagaaa agaccttggt tgctcgcgcc 1200 cgtcgtatcc agttcttctt gtcacaaaac ttcaacgttg cggaacaatt tactggtcag 1260 ccaggttctt atgttccagt tgctgaaact gtacgtggct ttaaggaaat ccttgatggt 1320 aaatacgacc acttgccaga agatgccttc cgtggtgtag gttctatcga agatgtgatt 1380 gcaaaagctg aaaaaatggg attt 1404 <210> 130 <211> 468 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (468) <223> Proton-translocating ATPase, F1 sector, beta-subunit <400> 130 Met Ser Ser Gly Lys Ile Ala Gln Val Ile Gly Pro Val Val Asp Val 1 5 10 15 Leu Phe Ala Ala Gly Glu Lys Leu Pro Glu Ile Asn Asn Ala Leu Val 20 25 30 Val Tyr Lys Asn Asp Glu Arg Lys Thr Lys Ile Val Leu Glu Val Ala 35 40 45 Leu Glu Leu Gly Asp Gly Met Val Arg Thr Ile Ala Met Glu Ser Thr 50 55 60 Asp Gly Leu Thr Arg Gly Met Glu Val Leu Asp Thr Gly Arg Pro Ile 65 70 75 80 Ser Val Pro Val Gly Lys Glu Thr Leu Gly Arg Val Phe Asn Val Leu 85 90 95 Gly Asp Thr Ile Asp Leu Glu Ala Pro Phe Thr Glu Asp Ala Glu Arg 100 105 110 Gln Pro Ile His Lys Lys Ala Pro Thr Phe Asp Glu Leu Ser Thr Ser 115 120 125 Ser Glu Ile Leu Glu Thr Gly Ile Lys Val Ile Asp Leu Leu Ala Pro 130 135 140 Tyr Leu Lys Gly Gly Lys Val Gly Leu Phe Gly Gly Ala Gly Val Gly 145 150 155 160 Lys Thr Val Leu Ile Gln Glu Leu Ile His Asn Ile Ala Gln Glu His 165 170 175 Gly Gly Ile Ser Val Phe Ala Gly Val Gly Glu Arg Thr Arg Glu Gly 180 185 190 Asn Asp Leu Tyr Trp Glu Met Lys Glu Ser Gly Val Ile Glu Lys Thr 195 200 205 Ala Met Val Phe Gly Gln Met Asn Glu Pro Pro Gly Ala Arg Met Arg 210 215 220 Val Ala Leu Thr Gly Leu Thr Ile Ala Glu Tyr Phe Arg Asp Val Glu 225 230 235 240 Gly Gln Asp Val Leu Leu Phe Ile Asp Asn Ile Phe Arg Phe Thr Gln 245 250 255 Ala Gly Ser Glu Val Ser Ala Leu Leu Gly Arg Met Pro Ser Ala Val 260 265 270 Gly Tyr Gln Pro Thr Leu Ala Thr Glu Met Gly Gln Leu Gln Glu Arg 275 280 285 Ile Thr Ser Thr Lys Lys Gly Ser Val Thr Ser Ile Gln Ala Ile Tyr 290 295 300 Val Pro Ala Asp Asp Tyr Thr Asp Pro Ala Pro Ala Thr Ala Phe Ala 305 310 315 320 His Leu Asp Ser Thr Thr Asn Leu Glu Arg Lys Leu Val Gln Leu Gly 325 330 335 Ile Tyr Pro Ala Val Asp Pro Leu Ala Ser Ser Ser Arg Ala Leu Ala 340 345 350 Pro Glu Ile Val Gly Glu Glu His Tyr Ala Val Ala Ala Glu Val Lys 355 360 365 Arg Val Leu Gln Arg Tyr His Glu Leu Gln Asp Ile Ila Ala Ile Leu 370 375 380 Gly Met Asp Glu Leu Ser Asp Glu Glu Lys Thr Leu Val Ala Arg Ala 385 390 395 400 Arg Arg Ile Gln Phe Phe Leu Ser Gln Asn Phe Asn Val Ala Glu Gln 405 410 415 Phe Thr Gly Gln Pro Gly Ser Tyr Val Pro Val Ala Glu Thr Val Arg 420 425 430 Gly Phe Lys Glu Ile Leu Asp Gly Lys Tyr Asp His Leu Pro Glu Asp 435 440 445 Ala Phe Arg Gly Val Gly Ser Ile Glu Asp Val Ile Ala Lys Ala Glu 450 455 460 Lys Met Gly Phe 465 <210> 131 <211> 876 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (876) <223> Streptococcus pneumoniae spr1361 gene <400> 131 atggcagtat ctctaaatga tattaaaaca aaaatcgcct caacaaaaaa tacgagtcaa 60 atcactaatg ccatgcaaat ggtatcggct gctaagctag gtcgttctga agaagctgct 120 cgcaacttcc aagtttacgc tcagaaagtg cgtaaacttt tgacagatat ccttcatggt 180 aatggagctg gtgcttcaac taatccgatg ttgattagcc gttctgtgaa gaagacaggc 240 tatatcgtta tcacttcaga ccgcggtttg gttggaggtt ataattcctc tattttgaaa 300 gctgttatgg agttgaaaga agaataccac ccagacggta aaggttttga aatgatctgt 360 atcggtggga tgggagctga tttctttaag gctcgcggta ttcaaccact ttatgaatta 420 cgtggcttgt cagaccaacc tagctttgat caagttcgta agattatttc aaaaactgtt 480 gaaatgtacc aaaatgaact ctttgatgag ctttatgttt gctacaacca ccatgtcaat 540 acgctaacca gtcaaatgcg tgtggaacaa atgcttccga ttgttgactt ggatccaaat 600 gaagcggatg aagagtacag cttgactttt gaattggaaa ccagccgaga agaaattctg 660 gagcagttgt tgcctcagtt tgcagaaagt atgatttacg gtgccattat cgatgccaag 720 acagctgaga atgctgcggg catgacagcc atgcaaacag cgacagataa tgctaagaaa 780 gtcatcaatg atttgacaat tcagtataac cgtgccagac aggcggcgat tacacaagaa 840 attacagaaa tcgtagcagg tgctagtgcc ttagaa 876 <210> 132 <211> 292 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (292) <223> Proton-translocating ATPase, F1 sector, gamma-subunit <400> 132 Met Ala Val Ser Leu Asn Asp Ile Lys Thr Lys Ile Ala Ser Thr Lys 1 5 10 15 Asn Thr Ser Gln Ile Thr Asn Ala Met Gln Met Val Ser Ala Ala Lys 20 25 30 Leu Gly Arg Ser Glu Glu Ala Ala Arg Asn Phe Gln Val Tyr Ala Gln 35 40 45 Lys Val Arg Lys Leu Leu Thr Asp Ile Leu His Gly Asn Gly Ala Gly 50 55 60 Ala Ser Thr Asn Pro Met Leu Ile Ser Arg Ser Val Lys Lys Thr Gly 65 70 75 80 Tyr Ile Val Ile Thr Ser Asp Arg Gly Leu Val Gly Gly Tyr Asn Ser 85 90 95 Ser Ile Leu Lys Ala Val Met Glu Leu Lys Glu Glu Tyr His Pro Asp 100 105 110 Gly Lys Gly Phe Glu Met Ile Cys Ile Gly Gly Met Gly Ala Asp Phe 115 120 125 Phe Lys Ala Arg Gly Ile Gln Pro Leu Tyr Glu Leu Arg Gly Leu Ser 130 135 140 Asp Gln Pro Ser Phe Asp Gln Val Arg Lys Ile Ile Ser Lys Thr Val 145 150 155 160 Glu Met Tyr Gln Asn Glu Leu Phe Asp Glu Leu Tyr Val Cys Tyr Asn 165 170 175 His His Val Asn Thr Leu Thr Ser Gln Met Arg Val Glu Gln Met Leu 180 185 190 Pro Ile Val Asp Leu Asp Pro Asn Glu Ala Asp Glu Glu Tyr Ser Leu 195 200 205 Thr Phe Glu Leu Glu Thr Ser Arg Glu Glu Ile Leu Glu Gln Leu Leu 210 215 220 Pro Gln Phe Ala Glu Ser Met Ile Tyr Gly Ala Ile Ile Asp Ala Lys 225 230 235 240 Thr Ala Glu Asn Ala Ala Gly Met Thr Ala Met Gln Thr Ala Thr Asp 245 250 255 Asn Ala Lys Lys Val Ile Asn Asp Leu Thr Ile Gln Tyr Asn Arg Ala 260 265 270 Arg Gln Ala Ala Ile Thr Gln Glu Ile Thr Glu Ile Val Ala Gly Ala 275 280 285 Ser Ala Leu Glu 290 <210> 133 <211> 1503 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1503) <223> Streptococcus pneumoniae spr1362 gene <133> 133 ttggcaatta acgcacaaga aatcagcgct ttaattaagc aacaaattga aaatttcaaa 60 cccaattttg atgtgactga aacaggtgtt gtaacctata tcggggacgg tatcgcgcgt 120 gctcacggcc ttgaaaatgt catgagtgga gagttgttga attttgaaaa cggctcttat 180 ggtatggctc aaaacttgga gtcaacagac gttggtatta tcatcctagg tgactttaca 240 gatatccgtg aaggcgatac aatccgccgt acagggaaaa tcatggaagt ccctgtaggt 300 gaaagtctga ttggtcgtgt tgtggatccg cttggtcgtc cagttgacgg tcttggagaa 360 atccacactg ataaaactcg tccagtagaa gcaccagctc ctggtgttat gcaacgtaag 420 tctgtttcag aaccattgca aactggtttg aaagctattg acgcccttgt accgattggt 480 cgtggtcaac gtgagttgat tatcggtgac cgtcagacag ggaaaacaac cattgcgatt 540 gatacaatct tgaaccaaaa agatcaagat atgatctgta tctacgtcgc gattggacaa 600 aaagaatcaa cagttcgtac gcaagtagaa acacttcgtc agtacggtgc cttggactac 660 acaatcgttg tgacagcctc tgcttcacaa ccatctccat tgctcttcct agctccttat 720 gctggggttg ctatggcgga agaatttatg tatcaaggta agcatgtttt gattgtatac 780 gatgatctat caaaacaagc ggtagcttat cgtgaactgt cgctcttgct tcgtcgtcct 840 ccaggtcgtg aagccttccc aggggatgtt ttctatctcc acagccgttt gcttgagcgc 900 tcagctaaag tttctgatga acttggtggt ggatcaatta cagccctacc atttatcgag 960 acacaagcag gagatatctc agcctatatc gcaaccaacg tgatttctat cactgatgga 1020 caaatcttcc ttggcgatgg cctcttcaat gcaggtattc gtccagccat cgatgcgggt 1080 tcatctgtat ctcgtgtagg tggttctgca caaatcaaag ccatgaagaa ggttgctggt 1140 acacttcgta tcgaccttgc ttcataccgt gagttggaag cctttactaa gtttggttct 1200 gacttggacg cagcaacaca ggctaagttg aaccgtggac gtcgtaccgt tgaggtcttg 1260 aaacaacctg ttcacaaacc attacctgtt gagaaacaag taaccattct ttatgctttg 1320 acacatggtt tcttggatac tgttccagta gatgatattg ttcgtttcga ggaagagttc 1380 catgccttct ttgatgctca acatccagag attttggaaa ccattcgtga tacaaaagac 1440 ttgccagaag aagcagtctt ggatgctgcg attacagagt ttctcaatca atctagcttc 1500 caa 1503 <210> 134 <211> 501 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (501) <223> Proton-translocating ATPase, F1 sector, alpha-subunit <400> 134 Met Ala Ile Asn Ala Gln Glu Ile Ser Ala Leu Ile Lys Gln Gln Ile 1 5 10 15 Glu Asn Phe Lys Pro Asn Phe Asp Val Thr Glu Thr Gly Val Val Thr 20 25 30 Tyr Ile Gly Asp Gly Ile Ala Arg Ala His Gly Leu Glu Asn Val Met 35 40 45 Ser Gly Glu Leu Leu Asn Phe Glu Asn Gly Ser Tyr Gly Met Ala Gln 50 55 60 Asn Leu Glu Ser Thr Asp Val Gly Ile Ile Leu Gly Asp Phe Thr 65 70 75 80 Asp Ile Arg Glu Gly Asp Thr Ile Arg Arg Thr Gly Lys Ile Met Glu 85 90 95 Val Pro Val Gly Glu Ser Leu Ile Gly Arg Val Val Asp Pro Leu Gly 100 105 110 Arg Pro Val Asp Gly Leu Gly Glu Ile His Thr Asp Lys Thr Arg Pro 115 120 125 Val Glu Ala Pro Ala Pro Gly Val Met Gln Arg Lys Ser Val Ser Glu 130 135 140 Pro Leu Gln Thr Gly Leu Lys Ala Ile Asp Ala Leu Val Pro Ile Gly 145 150 155 160 Arg Gly Gln Arg Glu Leu Ile Ile Gly Asp Arg Gln Thr Gly Lys Thr 165 170 175 Thr Ile Ala Ile Asp Thr Ile Leu Asn Gln Lys Asp Gln Asp Met Ile 180 185 190 Cys Ile Tyr Val Ala Ile Gly Gln Lys Glu Ser Thr Val Arg Thr Gln 195 200 205 Val Glu Thr Leu Arg Gln Tyr Gly Ala Leu Asp Tyr Thr Ile Val Val 210 215 220 Thr Ala Ser Ala Ser Gln Pro Ser Pro Leu Leu Phe Leu Ala Pro Tyr 225 230 235 240 Ala Gly Val Ala Met Ala Glu Glu Phe Met Tyr Gln Gly Lys His Val 245 250 255 Leu Ile Val Tyr Asp Asp Leu Ser Lys Gln Ala Val Ala Tyr Arg Glu 260 265 270 Leu Ser Leu Leu Leu Arg Arg Pro Pro Gly Arg Glu Ala Phe Pro Gly 275 280 285 Asp Val Phe Tyr Leu His Ser Arg Leu Leu Glu Arg Ser Ala Lys Val 290 295 300 Ser Asp Glu Leu Gly Gly Gly Ser Ile Thr Ala Leu Pro Phe Ile Glu 305 310 315 320 Thr Gln Ala Gly Asp Ile Ser Ala Tyr Ile Ala Thr Asn Val Ile Ser 325 330 335 Ile Thr Asp Gly Gln Ile Phe Leu Gly Asp Gly Leu Phe Asn Ala Gly 340 345 350 Ile Arg Pro Ala Ile Asp Ala Gly Ser Ser Val Ser Arg Val Gly Gly 355 360 365 Ser Ala Gln Ile Lys Ala Met Lys Lys Val Ala Gly Thr Leu Arg Ile 370 375 380 Asp Leu Ala Ser Tyr Arg Glu Leu Glu Ala Phe Thr Lys Phe Gly Ser 385 390 395 400 Asp Leu Asp Ala Ala Thr Gln Ala Lys Leu Asn Arg Gly Arg Arg Thr 405 410 415 Val Glu Val Leu Lys Gln Pro Val His Lys Pro Leu Pro Val Glu Lys 420 425 430 Gln Val Thr Ile Leu Tyr Ala Leu Thr His Gly Phe Leu Asp Thr Val 435 440 445 Pro Val Asp Asp Ile Val Arg Phe Glu Glu Glu Phe His Ala Phe Phe 450 455 460 Asp Ala Gln His Pro Glu Ile Leu Glu Thr Ile Arg Asp Thr Lys Asp 465 470 475 480 Leu Pro Glu Glu Ala Val Leu Asp Ala Ala Ile Thr Glu Phe Leu Asn 485 490 495 Gln Ser Ser Phe Gln 500 <210> 135 <211> 492 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (492) <223> Streptococcus pneumoniae spr1364 gene <400> 135 atgcacgtaa cagtaggtga attaattggt aattttattt taatcactgg ctcttttatt 60 cttttgctag tcttgattaa aaaatttgca tggtctaata ttacaggcat tttcgaagaa 120 agagctgaaa aaattgcttc agatattgac agagctgaag aagcccgtca aaaagcagaa 180 gtattggctc aaaaacgcga agatgaattg gctggtagcc gtaaagaagc taagacaatc 240 attgaaaatg caaaggaaac agctgagcaa agtaaggcta atatcttagc agatgctaaa 300 ctagaagcgg gacacttaaa agaaaaagcc aatcaagaaa ttgctcaaaa taaagtagaa 360 gctttacaga gtgttaaggg tgaggtcgca gatttgacca tcagcttagc tggtaaaatc 420 atctcacaaa accttgacag tcatgcccat aaagcactca ttgatcagta tatcgatcag 480 ctaggagaag ct 492 <210> 136 <211> 164 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (164) <223> Proton-translocating ATPase, F0 sector, subunit b <400> 136 Met His Val Thr Val Gly Glu Leu Ile Gly Asn Phe Ile Leu Ile Thr 1 5 10 15 Gly Ser Phe Ile Leu Leu Leu Val Leu Ile Lys Lys Phe Ala Trp Ser 20 25 30 Asn Ile Thr Gly Ile Phe Glu Glu Arg Ala Glu Lys Ile Ala Ser Asp 35 40 45 Ile Asp Arg Ala Glu Glu Ala Arg Gln Lys Ala Glu Val Leu Ala Gln 50 55 60 Lys Arg Glu Asp Glu Leu Ala Gly Ser Arg Lys Glu Ala Lys Thr Ile 65 70 75 80 Ile Glu Asn Ala Lys Glu Thr Ala Glu Gln Ser Lys Ala Asn Ile Leu 85 90 95 Ala Asp Ala Lys Leu Glu Ala Gly His Leu Lys Glu Lys Ala Asn Gln 100 105 110 Glu Ile Ala Gln Asn Lys Val Glu Ala Leu Gln Ser Val Lys Gly Glu 115 120 125 Val Ala Asp Leu Thr Ile Ser Leu Ala Gly Lys Ile Ile Ser Gln Asn 130 135 140 Leu Asp Ser His Ala His Lys Ala Leu Ile Asp Gln Tyr Ile Asp Gln 145 150 155 160 Leu Gly Glu Ala <210> 137 <211> 789 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (789) <223> Streptococcus pneumoniae spr1409 gene <400> 137 atgtcagcat atcaattacc gaccgtatgg caggatgaag ctagtaatca aggagctttt 60 acggggctaa acagaccaac agcaggtgcc cgtttcgaac aaaacttgcc aaaaggagaa 120 caagcttttc agctttattc actgggaaca ccaaatggtg tgaaggttac tatcttattg 180 gaagaattac tagaagctgg ttttaaggaa gcggcttacg acttgtataa gattgctatc 240 atggatgggg atcaattcgg atcagacttt gtgaagctca atccaaattc caagattcca 300 gccttattgg accagtcagg tactgaagat gtaagagtct ttgagtctgc tcatattctt 360 ctttaccttg ctgagaaatt tggagccttt ttaccaagta atcctgtgga aaaggtagaa 420 gttttgaatt ggctattctg gcaagcaggt gcagcacctt ttctaggtgg gggatttgga 480 catttcttca attatgctcc tgaaaaattg gaatatccta ttaaccgttt tacgatggaa 540 gtgaaacgtc agttggattt attggataag gaattggctc agaaacctta tattgcaggc 600 aatgactata cgattgcaga tattgctatc tggtcttggt atggacagtt agttcaagga 660 aatctttacc aaggttctgc aaaattcttg gatgcctcaa gttatcaaaa tctagtaaaa 720 tgggcagaaa aaattgccaa tcgtccagct gttaagcgtg gcttggaagt aacttataca 780 gaaattaaa 789 <210> 138 <211> 263 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (263) <223> Conserved hypothetical protein <400> 138 Met Ser Ala Tyr Gln Leu Pro Thr Val Trp Gln Asp Glu Ala Ser Asn 1 5 10 15 Gln Gly Ala Phe Thr Gly Leu Asn Arg Pro Thr Ala Gly Ala Arg Phe 20 25 30 Glu Gln Asn Leu Pro Lys Gly Glu Gln Ala Phe Gln Leu Tyr Ser Leu 35 40 45 Gly Thr Pro Asn Gly Val Lys Val Thr Ile Leu Leu Glu Glu Leu Leu 50 55 60 Glu Ala Gly Phe Lys Glu Ala Ala Tyr Asp Leu Tyr Lys Ile Ala Ile 65 70 75 80 Met Asp Gly Asp Gln Phe Gly Ser Asp Phe Val Lys Leu Asn Pro Asn 85 90 95 Ser Lys Ile Pro Ala Leu Leu Asp Gln Ser Gly Thr Glu Asp Val Arg 100 105 110 Val Phe Glu Ser Ala His Ile Leu Leu Tyr Leu Ala Glu Lys Phe Gly 115 120 125 Ala Phe Leu Pro Ser Asn Pro Val Glu Lys Val Glu Val Leu Asn Trp 130 135 140 Leu Phe Trp Gln Ala Gly Ala Ala Pro Phe Leu Gly Gly Gly Phe Gly 145 150 155 160 His Phe Phe Asn Tyr Ala Pro Glu Lys Leu Glu Tyr Pro Ile Asn Arg 165 170 175 Phe Thr Met Glu Val Lys Arg Gln Leu Asp Leu Leu Asp Lys Glu Leu 180 185 190 Ala Gln Lys Pro Tyr Ile Ala Gly Asn Asp Tyr Thr Ile Ala Asp Ile 195 200 205 Ala Ile Trp Ser Trp Tyr Gly Gln Leu Val Gln Gly Asn Leu Tyr Gln 210 215 220 Gly Ser Ala Lys Phe Leu Asp Ala Ser Ser Tyr Gln Asn Leu Val Lys 225 230 235 240 Trp Ala Glu Lys Ile Ala Asn Arg Pro Ala Val Lys Arg Gly Leu Glu 245 250 255 Val Thr Tyr Thr Glu Ile Lys 260 <139> <211> 2694 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (2694) <223> Streptococcus pneumoniae spr1410 gene <400> 139 atgtcaaaag aacaaaaacg ccaagcgttt tatactcaga gccctgaaga ggtcttgcag 60 gctgtggatg cgaccgagca aggtttgtca tcaagtgagg cggaaaagcg ccttgccgaa 120 tttgggcaca atgaactcga agaaggcgag aaacgatcaa tcttggtcaa attcatcgag 180 caatttaagg atttgatgat tatcatccta gttgcggcag caatcttgtc agtcgtgact 240 tctggtgggg aagatatcgc agatgccatt atcatcctag ctgtggttat catcaacgct 300 gcctttggtg tttaccaaga aggaaaagct gaagaagcta ttgaagccct caaatccatg 360 tctagtccag ttgcccgcgt tcttcgtgat ggacacatgg cagagattga ctctaaagaa 420 ttggtaccag gcgatatcgt tgcccttgaa gcaggtgacg tggtaccagc ggacctacgt 480 ttgatagaag ccaactctct taaaattgaa gaagcagcct tgacaggtga atctgtacca 540 gtcgaaaaag acttgtcagt cgatcttgcg acagatgctg gcattggtga ccgtgtcaac 600 atggccttcc aaaactcaaa cgtaacctat ggtcgtggga tgggtgttgt tgtcaataca 660 ggtatgtata ctgaagttgg tcatattgct ggtatgcttc aagatgcgga tgagactgat 720 acaccactca aacaaaactt gaacaacctt tctaaggtct tgacctatgc tatcttggtc 780 attgcccttg ttacttttgt agtgggtgtc ttcattcaag ggaaaaatcc acttggtgag 840 ttgttgactt ctgttgccct tgctgttgca gccattccag aaggacttcc tgctatcgtt 900 accatcgttc tttctcttgg tactcaagtt ttggccaaac gacattccat cgttcgtaag 960 ttgccagcag ttgaaacact tggttcaact gaaatcatcg cttctgataa gactggtacg 1020 ctgactatga acaagatgac agtcgaaaaa gtcttctacg atgcggttct acatgactca 1080 gctgatgata ttgaactagg tcttgaaatg ccactacttc gttcagttgt cttggccaat 1140 gatacgaaaa tcgatgtgga aggtaacttg attggtgacc caaccgaaac agcctttatc 1200 caatatgcct tggacaaggg ctatgatgtc aaaggtttct tagagaaata tcctcgtgta 1260 gctgaattgc catttgactc tgaccgtaag ctcatgtcaa cagttcaccc attgccagat 1320 agtcgtttcc ttgtagcagt caagggtgcg ccagaccaac tcttaaaacg ttgtcttctt 1380 cgtgataagg ctggggatat tgctccgatt gatgagaagg ttacaaatct cattcataca 1440 aacaattctg aaatggctca tcaagccttg cgtgtccttg caggtgctta taagattatc 1500 gatagtattc cagaaaatct cacttctgaa gagcttgaaa atgatttaat ttttactggt 1560 ttgattggga tgattgaccc tgaacgtcct gaagccgctg aggctgttcg tgtggctaag 1620 gaagcgggaa tccgtccaat tatgattaca ggtgaccacc aagacacagc ggaagccatt 1680 gccaaacgtt tgggaatcat tgacgcaaac gatacagaag gtcacgtttt aactggtgct 1740 gaactcaatg aactgtcaga tgaagaattt gaaaaagttg ttggtcaata ctctgtttat 1800 gcccgtgtgt ctccagaaca caaggttcgt atcgtcaagg cttggcaaaa acaaggtaaa 1860 gtcgttgcca tgacaggtga cggtgtcaat gacgcgccag ctctgaaaac agccgatatt 1920 ggtatcggta tgggaatcac tggtacagag gtttctaagg gggcttctga tatgattctt 1980 gcagatgata actttgcgac tattatcgtc gcagtggaag aaggacgtaa ggtcttctca 2040 aacattcaaa agactattca gtacctactt tctgctaata ctgctgaagt attaaccatc 2100 ttcctatcaa ccttgtttgg ttgggatgtc ttgcagccgg ttcatctttt gtggatcaac 2160 ttggtaacgg ataccttccc agctatcgct cttggtgttg aacctgcgga acctggtgtc 2220 atgaatcata aaccacgtgg acgcaaggca agcttcttct caggtggtgt tttgagttct 2280 atcatttatc aaggtgtact ccaagcagct cttgttatga gtgtttatgg ccttgcgatt 2340 gcttacccag ttcatgtggg tgacaatcat gctattcatg cagatgccct aacgatggcc 2400 tttgcaaccc ttggtttgat tcagctcttc catgcctaca atgtcaagtc tgtctaccaa 2460 tccatcttga cagttggccc attcaagtct aagaccttta actggtccat cttggtatcc 2520 tttatccttc tcatggcaac aatcgttgta gaaccgcttg aaggaatctt ccacgtaacc 2580 aaactagact tgtcacaatg gggaattgtt atggctggaa gcttctcaat gattatcatc 2640 gtcgaaatcg ttaagtttat tcaacgcaaa ctcggttttg acaagaatgc gatt 2694 <210> 140 <211> 898 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (898) <223> P-type ATPase-calcium transporter <400> 140 Met Ser Lys Glu Gln Lys Arg Gln Ala Phe Tyr Thr Gln Ser Pro Glu 1 5 10 15 Glu Val Leu Gln Ala Val Asp Ala Thr Glu Gln Gly Leu Ser Ser Ser 20 25 30 Glu Ala Glu Lys Arg Leu Ala Glu Phe Gly His Asn Glu Leu Glu Glu 35 40 45 Gly Glu Lys Arg Ser Ile Leu Val Lys Phe Ile Glu Gln Phe Lys Asp 50 55 60 Leu Met Ile Ile Ile Leu Val Ala Ala Ala Ile Leu Ser Val Val Thr 65 70 75 80 Ser Gly Gly Glu Asp Ile Ala Asp Ala Ile Ile Ile Leu Ala Val Val 85 90 95 Ile Ile Asn Ala Ala Phe Gly Val Tyr Gln Glu Gly Lys Ala Glu Glu 100 105 110 Ala Ile Glu Ala Leu Lys Ser Met Ser Ser Pro Val Ala Arg Val Leu 115 120 125 Arg Asp Gly His Met Ala Glu Ile Asp Ser Lys Glu Leu Val Pro Gly 130 135 140 Asp Ile Val Ala Leu Glu Ala Gly Asp Val Val Pro Ala Asp Leu Arg 145 150 155 160 Leu Ile Glu Ala Asn Ser Leu Lys Ile Glu Glu Ala Ala Leu Thr Gly 165 170 175 Glu Ser Val Pro Val Glu Lys Asp Leu Ser Val Asp Leu Ala Thr Asp 180 185 190 Ala Gly Ile Gly Asp Arg Val Asn Met Ala Phe Gln Asn Ser Asn Val 195 200 205 Thr Tyr Gly Arg Gly Met Gly Val Val Asn Thr Gly Met Tyr Thr 210 215 220 Glu Val Gly His Ile Ala Gly Met Leu Gln Asp Ala Asp Glu Thr Asp 225 230 235 240 Thr Pro Leu Lys Gln Asn Leu Asn Asn Leu Ser Lys Val Leu Thr Tyr 245 250 255 Ala Ile Leu Val Ile Ala Leu Val Thr Phe Val Val Gly Val Phe Ile 260 265 270 Gln Gly Lys Asn Pro Leu Gly Glu Leu Leu Thr Ser Val Ala Leu Ala 275 280 285 Val Ala Ala Ile Pro Glu Gly Leu Pro Ala Ile Val Thr Ile Val Leu 290 295 300 Ser Leu Gly Thr Gln Val Leu Ala Lys Arg His Ser Ile Val Arg Lys 305 310 315 320 Leu Pro Ala Val Glu Thr Leu Gly Ser Thr Glu Ile Ile Ala Ser Asp 325 330 335 Lys Thr Gly Thr Leu Thr Met Asn Lys Met Thr Val Glu Lys Val Phe 340 345 350 Tyr Asp Ala Val Leu His Asp Ser Ala Asp Asp Ile Glu Leu Gly Leu 355 360 365 Glu Met Pro Leu Leu Arg Ser Val Val Leu Ala Asn Asp Thr Lys Ile 370 375 380 Asp Val Glu Gly Asn Leu Ile Gly Asp Pro Thr Glu Thr Ala Phe Ile 385 390 395 400 Gln Tyr Ala Leu Asp Lys Gly Tyr Asp Val Lys Gly Phe Leu Glu Lys 405 410 415 Tyr Pro Arg Val Ala Glu Leu Pro Phe Asp Ser Asp Arg Lys Leu Met 420 425 430 Ser Thr Val His Pro Leu Pro Asp Ser Arg Phe Leu Val Ala Val Lys 435 440 445 Gly Ala Pro Asp Gln Leu Leu Lys Arg Cys Leu Leu Arg Asp Lys Ala 450 455 460 Gly Asp Ile Ala Pro Ile Asp Glu Lys Val Thr Asn Leu Ile His Thr 465 470 475 480 Asn Asn Ser Glu Met Ala His Gln Ala Leu Arg Val Leu Ala Gly Ala 485 490 495 Tyr Lys Ile Ile Asp Ser Ile Pro Glu Asn Leu Thr Ser Glu Glu Leu 500 505 510 Glu Asn Asp Leu Ile Phe Thr Gly Leu Ile Gly Met Ile Asp Pro Glu 515 520 525 Arg Pro Glu Ala Ala Glu Ala Val Arg Val Ala Lys Glu Ala Gly Ile 530 535 540 Arg Pro Ile Met Ile Thr Gly Asp His Gln Asp Thr Ala Glu Ala Ile 545 550 555 560 Ala Lys Arg Leu Gly Ile Ile Asp Ala Asn Asp Thr Glu Gly His Val 565 570 575 Leu Thr Gly Ala Glu Leu Asn Glu Leu Ser Asp Glu Glu Phe Glu Lys 580 585 590 Val Val Gly Gln Tyr Ser Val Tyr Ala Arg Val Ser Pro Glu His Lys 595 600 605 Val Arg Ile Val Lys Ala Trp Gln Lys Gln Gly Lys Val Val Ala Met 610 615 620 Thr Gly Asp Gly Val Asn Asp Ala Pro Ala Leu Lys Thr Ala Asp Ile 625 630 635 640 Gly Ile Gly Met Gly Ile Thr Gly Thr Glu Val Ser Lys Gly Ala Ser 645 650 655 Asp Met Ile Leu Ala Asp Asp Asn Phe Ala Thr Ile Ile Val Ala Val 660 665 670 Glu Glu Gly Arg Lys Val Phe Ser Asn Ile Gln Lys Thr Ile Gln Tyr 675 680 685 Leu Leu Ser Ala Asn Thr Ala Glu Val Leu Thr Ile Phe Leu Ser Thr 690 695 700 Leu Phe Gly Trp Asp Val Leu Gln Pro Val His Leu Leu Trp Ile Asn 705 710 715 720 Leu Val Thr Asp Thr Phe Pro Ala Ile Ala Leu Gly Val Glu Pro Ala 725 730 735 Glu Pro Gly Val Met Asn His Lys Pro Arg Gly Arg Lys Ala Ser Phe 740 745 750 Phe Ser Gly Gly Val Leu Ser Ser Ile Tyr Gln Gly Val Leu Gln 755 760 765 Ala Ala Leu Val Met Ser Val Tyr Gly Leu Ala Ile Ala Tyr Pro Val 770 775 780 His Val Gly Asp Asn His Ala Ile His Ala Asp Ala Leu Thr Met Ala 785 790 795 800 Phe Ala Thr Leu Gly Leu Ile Gln Leu Phe His Ala Tyr Asn Val Lys 805 810 815 Ser Val Tyr Gln Ser Ile Leu Thr Val Gly Pro Phe Lys Ser Lys Thr 820 825 830 Phe Asn Trp Ser Ile Leu Val Ser Phe Ile Leu Leu Met Ala Thr Ile 835 840 845 Val Val Glu Pro Leu Glu Gly Ile Phe His Val Thr Lys Leu Asp Leu 850 855 860 Ser Gln Trp Gly Ile Val Met Ala Gly Ser Phe Ser Met Ile Ile Ile 865 870 875 880 Val Glu Ile Val Lys Phe Ile Gln Arg Lys Leu Gly Phe Asp Lys Asn 885 890 895 Ala ile <210> 141 <211> 1182 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1182) <223> Streptococcus pneumoniae spr1411 gene <400> 141 atgaagcaat ctatctcaaa tctcaagtta gctgagcgtg gagccattat cagtatttcg 60 acctatttga tcttgtctgc agccaaatta gcagctggtc atctccttca ttcatccagt 120 ttggtggccg atggttttaa taacgtatcg gacatcattg gaaatgtggc cctcttaatc 180 gggattcgga tggcgcgcca gcctgcagac cgtgaccacc gttttggtca ttggaagatt 240 gaagatttgg caagcttgat cacttctatc atcatgttct atgtcggttt cgatgttcta 300 agagatacca ttcaaaagat tctcagtcgg gaagaatcgg tcattgatcc tcttggtgca 360 actctaggaa tcatttctgc agcgattatg tttgtggtct atctctacaa tactcgcctc 420 agtaagaaat ccaactccaa agcgctgaag gcagctgcta aggacaatct ttctgacgct 480 gttacctcac ttggaaccgc cattgccatc ctagctagta gtttcaatta tccgattgtg 540 gataaactgg ttgctatcat catcactttc tttatcttga agactgccta tgatatcttc 600 atcgagtctt cctttagtct ttcagatggc tttgacgacc gcctgctcga ggactaccaa 660 aaggctatca tggaaattcc caaaatcagc aaggtcaaat cgcaaagagg tcgcacctac 720 ggtagcaaca tctacctgga tattacacta gagatgaatc ctgacttgtc tgtttttgaa 780 agccatgaaa tcgcggatca ggtcgagtct atgctggaga atcgttttgg cgtctttgat 840 accgatgtcc atatcgaacc agcacctatc cctgaggatg aaattttaga caatgtctat 900 aaaaaattgc ttatgcgtga acaattgatc gaccaaggaa accaactaga agaactcttg 960 actgatgatt ttgtctatat tcgccaagat ggagagcaga tggataaaga ggcttataag 1020 accaaaaaag agttaaattc tgctatcaag gacattcaaa ttacttccat cagtcaaaaa 1080 accaaactta tctgctatga gttagatggt atcatccata ccagtatctg gcgtcgccac 1140 gaaacctggc aaaatatctt tcatcaagaa accaaaaaag aa 1182 <210> 142 <211> 394 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) ... (394) <223> Conserved hypothetical protein <400> 142 Met Lys Gln Ser Ile Ser Asn Leu Lys Leu Ala Glu Arg Gly Ala Ile 1 5 10 15 Ile Ser Ile Ser Thr Tyr Leu Ile Leu Ser Ala Ala Lys Leu Ala Ala 20 25 30 Gly His Leu Leu His Ser Ser Ser Leu Val Ala Asp Gly Phe Asn Asn 35 40 45 Val Ser Asp Ile Ile Gly Asn Val Ala Leu Leu Ile Gly Ile Arg Met 50 55 60 Ala Arg Gln Pro Ala Asp Arg Asp His Arg Phe Gly His Trp Lys Ile 65 70 75 80 Glu Asp Leu Ala Ser Leu Ile Thr Ser Ile Met Phe Tyr Val Gly 85 90 95 Phe Asp Val Leu Arg Asp Thr Ile Gln Lys Ile Leu Ser Arg Glu Glu 100 105 110 Ser Val Ile Asp Pro Leu Gly Ala Thr Leu Gly Ile Ile Ser Ala Ala 115 120 125 Ile Met Phe Val Val Tyr Leu Tyr Asn Thr Arg Leu Ser Lys Lys Ser 130 135 140 Asn Ser Lys Ala Leu Lys Ala Ala Ala Lys Asp Asn Leu Ser Asp Ala 145 150 155 160 Val Thr Ser Leu Gly Thr Ala Ile Ala Ile Leu Ala Ser Ser Phe Asn 165 170 175 Tyr Pro Ile Val Asp Lys Leu Val Ala Ile Ile Ile Thr Phe Phe Ile 180 185 190 Leu Lys Thr Ala Tyr Asp Ile Phe Ile Glu Ser Ser Phe Ser Leu Ser 195 200 205 Asp Gly Phe Asp Asp Arg Leu Leu Glu Asp Tyr Gln Lys Ala Ile Met 210 215 220 Glu Ile Pro Lys Ile Ser Lys Val Lys Ser Gln Arg Gly Arg Thr Tyr 225 230 235 240 Gly Ser Asn Ile Tyr Leu Asp Ile Thr Leu Glu Met Asn Pro Asp Leu 245 250 255 Ser Val Phe Glu Ser His Glu Ile Ala Asp Gln Val Glu Ser Met Leu 260 265 270 Glu Asn Arg Phe Gly Val Phe Asp Thr Asp Val His Ile Glu Pro Ala 275 280 285 Pro Ile Pro Glu Asp Glu Ile Leu Asp Asn Val Tyr Lys Lys Leu Leu 290 295 300 Met Arg Glu Gln Leu Ile Asp Gln Gly Asn Gln Leu Glu Glu Leu Leu 305 310 315 320 Thr Asp Asp Phe Val Tyr Ile Arg Gln Asp Gly Glu Gln Met Asp Lys 325 330 335 Glu Ala Tyr Lys Thr Lys Lys Glu Leu Asn Ser Ala Ile Lys Asp Ile 340 345 350 Gln Ile Thr Ser Ile Ser Gln Lys Thr Lys Leu Ile Cys Tyr Glu Leu 355 360 365 Asp Gly Ile Ile His Thr Ser Ile Trp Arg Arg His Glu Thr Trp Gln 370 375 380 Asn Ile Phe His Gln Glu Thr Lys Lys Glu 385 390 <210> 143 <211> 1197 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1197) <223> Streptococcus pneumoniae spr1413 gene <400> 143 atgagattaa cgcaaatgcc ttctgaattt cagaaggctt taccagtatt agaaaaaatt 60 aaagaagcag gctttgaggc ttattttgtt gggggctctg ttcgagatgc ccttctccat 120 agccctatcc acgatgtgga tattgcgacg tcttcttatc cagaagagac caagcagatt 180 tttccgcgaa cagccgatat cggaatcgag catggaaccg tcttggtctt agatggggat 240 gaggagtatg aggtaacaac ctttcggaca gaggatgtct atgtggacta tcgcagaccc 300 agtgcggttt cctttgtacg atcgctagaa gaagacctca aacgccgtga tttcacagtc 360 aacgcctttg ccttggatga gacaggagaa atcgttgact tgttccatgg tttagaagat 420 ttggaaaagc aagtcttgcg agcagttgga gtggctagtg agcgtttcaa cgaagatgct 480 ttacggatta tgcgtggttt ccgttttcag gctagtcttg gttttgcact tgagccagaa 540 acatttaaag ctatgaagac cttgacgccg cttttggaga aaatttctgt agagcgtacc 600 ttcgtcgagt ttgataaact cttgctggct ccattttgga gaaggggctt ggcttccatg 660 attgagagtc aagcttatga ctatctccct gatatggcat ctagccagga caagctcaac 720 agactgtttg atttggagac tgattttact tttgaatcct ctgaacaagc ctgggcggct 780 ttactatggg ctttggagat tgaaaatgcg cagtcatttt tgaaatcttg gaagacctca 840 cgccagtttg ccaagcaagt tcaggatttg ctgattattt tggctctgcg tgaaaatgga 900 gaattgagca agcgagattg ttatcgcttt gacatagatt tgcttttaca ggctgaaaat 960 cttcgtcaag ctcaaggaaa agaagtcaac ccacaagcca tcacagaaaa ataccaaagc 1020 ttgactatcc atgacaagaa agagattcag attaatggcg gtattttgat caaggaatat 1080 ggctatcagc caggcccaga cttgggagag attttaacag agattgagtt tgccattgtc 1140 gatggagaat tggagaataa tcgtgaagcc atccatgctt acctgaggga gaaaaaa 1197 <210> 144 <211> 399 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (399) <223> tRNA nucleotidyltransferase <400> 144 Met Arg Leu Thr Gln Met Pro Ser Glu Phe Gln Lys Ala Leu Pro Val 1 5 10 15 Leu Glu Lys Ile Lys Glu Ala Gly Phe Glu Ala Tyr Phe Val Gly Gly 20 25 30 Ser Val Arg Asp Ala Leu Leu His Ser Pro Ile His Asp Val Asp Ile 35 40 45 Ala Thr Ser Ser Tyr Pro Glu Glu Thr Lys Gln Ile Phe Pro Arg Thr 50 55 60 Ala Asp Ile Gly Ile Glu His Gly Thr Val Leu Val Leu Asp Gly Asp 65 70 75 80 Glu Glu Tyr Glu Val Thr Thr Phe Arg Thr Glu Asp Val Tyr Val Asp 85 90 95 Tyr Arg Arg Pro Ser Ala Val Ser Phe Val Arg Ser Leu Glu Glu Asp 100 105 110 Leu Lys Arg Arg Asp Phe Thr Val Asn Ala Phe Ala Leu Asp Glu Thr 115 120 125 Gly Glu Ile Val Asp Leu Phe His Gly Leu Glu Asp Leu Glu Lys Gln 130 135 140 Val Leu Arg Ala Val Gly Val Ala Ser Glu Arg Phe Asn Glu Asp Ala 145 150 155 160 Leu Arg Ile Met Arg Gly Phe Arg Phe Gln Ala Ser Leu Gly Phe Ala 165 170 175 Leu Glu Pro Glu Thr Phe Lys Ala Met Lys Thr Leu Thr Pro Leu Leu 180 185 190 Glu Lys Ile Ser Val Glu Arg Thr Phe Val Glu Phe Asp Lys Leu Leu 195 200 205 Leu Ala Pro Phe Trp Arg Arg Gly Leu Ala Ser Met Ile Glu Ser Gln 210 215 220 Ala Tyr Asp Tyr Leu Pro Asp Met Ala Ser Ser Gln Asp Lys Leu Asn 225 230 235 240 Arg Leu Phe Asp Leu Glu Thr Asp Phe Thr Phe Glu Ser Ser Glu Gln 245 250 255 Ala Trp Ala Ala Leu Leu Trp Ala Leu Glu Ile Glu Asn Ala Gln Ser 260 265 270 Phe Leu Lys Ser Trp Lys Thr Ser Arg Gln Phe Ala Lys Gln Val Gln 275 280 285 Asp Leu Leu Ile Ile Leu Ala Leu Arg Glu Asn Gly Glu Leu Ser Lys 290 295 300 Arg Asp Cys Tyr Arg Phe Asp Ile Asp Leu Leu Leu Gln Ala Glu Asn 305 310 315 320 Leu Arg Gln Ala Gln Gly Lys Glu Val Asn Pro Gln Ala Ile Thr Glu 325 330 335 Lys Tyr Gln Ser Leu Thr Ile His Asp Lys Lys Glu Ile Gln Ile Asn 340 345 350 Gly Gly Ile Leu Ile Lys Glu Tyr Gly Tyr Gln Pro Gly Pro Asp Leu 355 360 365 Gly Glu Ile Leu Thr Glu Ile Glu Phe Ala Ile Val Asp Gly Glu Leu 370 375 380 Glu Asn Asn Arg Glu Ala Ile His Ala Tyr Leu Arg Glu Lys Lys 385 390 395 <210> 145 <211> 876 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (876) <223> Streptococcus pneumoniae spr1419 gene <400> 145 ttgataggga ggatatcggg gatgaatttt caacaattat ccaatctgca atattggacc 60 agtttgtttg caagtccatg gacgatagct atcaatctga ttgatatttt gattgttgct 120 tatattttat accattttac aaaagctatt gcaggaacca agattatgat tttggtacgt 180 ggagttttgg tgtttatttt agcccaaatc cttgcaaata tgattggttt gactacgatt 240 tcttggttaa tcaatcaaat tattacttat ggggttattg cggcggttgt tatcttctct 300 ccagagattc ggactggttt ggaacgtttg ggaagagcga cagatttctt ttccaatgcc 360 cctattagtg ctgaggaaca gatgattcgt gcctttgtta agtctgttga atacatgagt 420 cctcgtaaaa tcggggcctt ggttgctatt cagcgtgtac gtaccttgca ggagtatatt 480 tcgacaggaa ttcccttgga tgctaagatt tctgcagaac ttctcattaa catttttatt 540 cccaacactc ccctacatga tggtgcggtg attatcaaag aagaacgtat cgctgtgacg 600 tctgcctatc tgcccttgac aaaaaacaca gggatttcca aggaatttgg gaccagacac 660 cgggcggcta tcggtttatc agaagtctca gatgccttga cttttgtcgt atcagaggaa 720 acgggaggaa tttcgataac ctataatgga aggtttaagc acaacctaac acttgatgaa 780 tttgaaacag aattacgtga aatcttactt ccaaaagagg aagtgggtct tagttttaaa 840 gaacgattgc taggaggatg gaaacatgaa aaaaaa 876 <210> 146 <211> 292 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (292) <223> Conserved hypothetical protein <400> 146 Met Ile Gly Arg Ile Ser Gly Met Asn Phe Gln Gln Leu Ser Asn Leu 1 5 10 15 Gln Tyr Trp Thr Ser Leu Phe Ala Ser Pro Trp Thr Ile Ala Ile Asn 20 25 30 Leu Ile Asp Ile Leu Ile Val Ala Tyr Ile Leu Tyr His Phe Thr Lys 35 40 45 Ala Ile Ala Gly Thr Lys Ile Met Ile Leu Val Arg Gly Val Leu Val 50 55 60 Phe Ile Leu Ala Gln Ile Leu Ala Asn Met Ile Gly Leu Thr Thr Ile 65 70 75 80 Ser Trp Leu Ile Asn Gln Ile Ile Thr Tyr Gly Val Ile Ala Ala Val 85 90 95 Val Ile Phe Ser Pro Glu Ile Arg Thr Gly Leu Glu Arg Leu Gly Arg 100 105 110 Ala Thr Asp Phe Phe Ser Asn Ala Pro Ile Ser Ala Glu Glu Gln Met 115 120 125 Ile Arg Ala Phe Val Lys Ser Val Glu Tyr Met Ser Pro Arg Lys Ile 130 135 140 Gly Ala Leu Val Ala Ile Gln Arg Val Arg Thr Leu Gln Glu Tyr Ile 145 150 155 160 Ser Thr Gly Ile Pro Leu Asp Ala Lys Ile Ser Ala Glu Leu Leu Ile 165 170 175 Asn Ile Phe Ile Pro Asn Thr Pro Leu His Asp Gly Ala Val Ile Ile 180 185 190 Lys Glu Glu Arg Ile Ala Val Thr Ser Ala Tyr Leu Pro Leu Thr Lys 195 200 205 Asn Thr Gly Ile Ser Lys Glu Phe Gly Thr Arg His Arg Ala Ala Ile 210 215 220 Gly Leu Ser Glu Val Ser Asp Ala Leu Thr Phe Val Val Ser Glu Glu 225 230 235 240 Thr Gly Gly Ile Ser Ile Thr Tyr Asn Gly Arg Phe Lys His Asn Leu 245 250 255 Thr Leu Asp Glu Phe Glu Thr Glu Leu Arg Glu Ile Leu Leu Pro Lys 260 265 270 Glu Glu Val Gly Leu Ser Phe Lys Glu Arg Leu Leu Gly Gly Trp Lys 275 280 285 His Glu Lys Lys 290 <210> 147 <211> 1230 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1230) <223> Streptococcus pneumoniae spr1427 gene <400> 147 atgtctacaa atagaaaaaa tgatatgatg gtttattgct cattttgtgg caaaaaccaa 60 gaagaagtac aaaaaataat tgctggcaac aatgctttta tttgtaatga atgcgtggag 120 ttagctcagg aaatcattcg agaagaattg gttgaggaag tcttggcaga cttgtctgag 180 gtgccaaaac caattgaact cctccatatc ttgaaccact atgtaattgg tcaagatcgt 240 gccaagcgtg ccttggcagt ggcggtttat aaccactaca aacgcatcaa tttccacgat 300 acacgcgaag agtcagaaga tgtggatttg cagaagtcaa acattttgat gattggccca 360 actggttcag ggaaaacttt ccttgcccag accttggcta agagcttgaa tgtgcctttt 420 gctattgcgg atgcgacagc tctgacggag gctggttatg tgggtgagga cgtggaaaat 480 atcctcctca aactcttgca ggttgctgac tttaacatcg aacgtgcaga gcgtggcatt 540 atctatgtgg atgaaattga caagattgcc aagaagagtg agaatgtgtc tatcacacgt 600 gatgtttctg gtgaaggggt gcaacaagcc cttctcaaga ttatcgaggg aactgttgct 660 agcgtaccgc ctcaaggtgg acgcaaacat ccacaacaag agatgattca agtggataca 720 aaaaatatcc tcttcatcgt gggtggtgct tttgatggta ttgaagaaat tgtcaaacaa 780 cgtctgggtg aaaaagtcat cggatttggt caaaacaata aggcgattga cgaaaacagc 840 tcatacatgc aagaaatcat cgctgaagac attcaaaaat ttggtattat ccctgagttg 900 attggacgct tgcctgtttt tgcggctctt gagcaattga ccgttgatga cttggttcgc 960 atcttgaaag agccaagaaa tgccttggtg aaacagtacc aaaccttgct ttcttatgat 1020 gatgttgagt tggaatttga cgacgaagcc cttcaagaga ttgctaataa agcaatcgaa 1080 cggaagacag gggcgcgtgg acttcgctcc atcatcgaag aaaccatgct agatgttatg 1140 tttgaggtgc cgagtcagga aaatgtgaaa ttggttcgca tcactaaaga aactgtcgat 1200 ggaacggata aaccgatcct agaaacagcc 1230 <210> 148 <211> 410 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (410) <223> ATP-dependent Clp protease ATP-binding subunit <400> 148 Met Ser Thr Asn Arg Lys Asn Asp Met Met Val Tyr Cys Ser Phe Cys 1 5 10 15 Gly Lys Asn Gln Glu Glu Val Gln Lys Ile Ile Ala Gly Asn Asn Ala 20 25 30 Phe Ile Cys Asn Glu Cys Val Glu Leu Ala Gln Glu Ile Ile Arg Glu 35 40 45 Glu Leu Val Glu Glu Val Leu Ala Asp Leu Ser Glu Val Pro Lys Pro 50 55 60 Ile Glu Leu Leu His Ile Leu Asn His Tyr Val Ile Gly Gln Asp Arg 65 70 75 80 Ala Lys Arg Ala Leu Ala Val Ala Val Tyr Asn His Tyr Lys Arg Ile 85 90 95 Asn Phe His Asp Thr Arg Glu Glu Ser Glu Asp Val Asp Leu Gln Lys 100 105 110 Ser Asn Ile Leu Met Ile Gly Pro Thr Gly Ser Gly Lys Thr Phe Leu 115 120 125 Ala Gln Thr Leu Ala Lys Ser Leu Asn Val Pro Phe Ala Ile Ala Asp 130 135 140 Ala Thr Ala Leu Thr Glu Ala Gly Tyr Val Gly Glu Asp Val Glu Asn 145 150 155 160 Ile Leu Leu Lys Leu Leu Gln Val Ala Asp Phe Asn Ile Glu Arg Ala 165 170 175 Glu Arg Gly Ile Ile Tyr Val Asp Glu Ile Asp Lys Ile Ala Lys Lys 180 185 190 Ser Glu Asn Val Ser Ile Thr Arg Asp Val Ser Gly Glu Gly Val Gln 195 200 205 Gln Ala Leu Leu Lys Ile Ile Glu Gly Thr Val Ala Ser Val Pro Pro 210 215 220 Gln Gly Gly Arg Lys His Pro Gln Gln Glu Met Ile Gln Val Asp Thr 225 230 235 240 Lys Asn Ile Leu Phe Ile Val Gly Gly Ala Phe Asp Gly Ile Glu Glu 245 250 255 Ile Val Lys Gln Arg Leu Gly Glu Lys Val Ile Gly Phe Gly Gln Asn 260 265 270 Asn Lys Ala Ile Asp Glu Asn Ser Ser Tyr Met Gln Glu Ile Ile Ala 275 280 285 Glu Asp Ile Gln Lys Phe Gly Ile Ile Pro Glu Leu Ile Gly Arg Leu 290 295 300 Pro Val Phe Ala Ala Leu Glu Gln Leu Thr Val Asp Asp Leu Val Arg 305 310 315 320 Ile Leu Lys Glu Pro Arg Asn Ala Leu Val Lys Gln Tyr Gln Thr Leu 325 330 335 Leu Ser Tyr Asp Asp Val Glu Leu Glu Phe Asp Asp Glu Ala Leu Gln 340 345 350 Glu Ile Ala Asn Lys Ala Ile Glu Arg Lys Thr Gly Ala Arg Gly Leu 355 360 365 Arg Ser Ile Ile Glu Glu Thr Met Leu Asp Val Met Phe Glu Val Pro 370 375 380 Ser Gln Glu Asn Val Lys Leu Val Arg Ile Thr Lys Glu Thr Val Asp 385 390 395 400 Gly Thr Asp Lys Pro Ile Leu Glu Thr Ala 405 410 <210> 149 <211> 504 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (504) <223> Streptococcus pneumoniae spr1429 gene <400> 149 atgactaaga aaatcgtagc tatttgggcc caggatgaag agggtttgat tggtaaggaa 60 aatcgtctgc cttggcattt gccagcagaa ttgcagcact ttaaagaaac aactctgaat 120 catgctatct tgatggggcg tgtgaccttt gatgggatgg ggcgtcgctt gcttccaaaa 180 cgggaaaccc tgattttgac gcgtaatccg gaagaaaaga tagatggggt tgctactttt 240 caggacgtcc agtctgttct tgactggtat caggatcaag aaaagaatct ctacattatc 300 ggtgggaagc aaatttttca ggcttttgaa ccttaccttg atgaagtgat tgtcactcac 360 attcatgctc gggtggaagg agatacctat ttccctgaag agcttgactt gtctcttttt 420 gagacagttt caagcaaatt ttacgccaaa gatgagaaga atccttatga ttttaccatc 480 caataccgca agagaaagga agtc 504 <210> 150 <211> 168 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (168) <223> Dihydrofolate reductase <400> 150 Met Thr Lys Lys Ile Val Ala Ile Trp Ala Gln Asp Glu Glu Gly Leu 1 5 10 15 Ile Gly Lys Glu Asn Arg Leu Pro Trp His Leu Pro Ala Glu Leu Gln 20 25 30 His Phe Lys Glu Thr Thr Leu Asn His Ala Ile Leu Met Gly Arg Val 35 40 45 Thr Phe Asp Gly Met Gly Arg Arg Leu Leu Pro Lys Arg Glu Thr Leu 50 55 60 Ile Leu Thr Arg Asn Pro Glu Glu Lys Ile Asp Gly Val Ala Thr Phe 65 70 75 80 Gln Asp Val Gln Ser Val Leu Asp Trp Tyr Gln Asp Gln Glu Lys Asn 85 90 95 Leu Tyr Ile Ile Gly Gly Lys Gln Ile Phe Gln Ala Phe Glu Pro Tyr 100 105 110 Leu Asp Glu Val Ile Val Thr His Ile His Ala Arg Val Glu Gly Asp 115 120 125 Thr Tyr Phe Pro Glu Glu Leu Asp Leu Ser Leu Phe Glu Thr Val Ser 130 135 140 Ser Lys Phe Tyr Ala Lys Asp Glu Lys Asn Pro Tyr Asp Phe Thr Ile 145 150 155 160 Gln Tyr Arg Lys Arg Lys Glu Val 165 <210> 151 <211> 534 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (534) <223> Streptococcus pneumoniae spr1430 gene <400> 151 atgaatgagg taaagaaaat ggtagaattg aaaaaagaag cagtaaaaga cgtaacatca 60 ttgacaaaag cagcgccagt agcattggca aaaacaaagg aagtcttgaa ccaagctgtt 120 gctgatttgt atgtagctca cgttgctttg caccaagtgc actggtatat gcatggtcgt 180 ggtttccttg tatggcatcc aaaaatggat gagtacatgg aagctcttga cggtcaattg 240 gatgaaatca gcgaacgctt gattacactc ggtggaagcc cattctctac attgacagag 300 ttccttcaaa atagtgaaat cgaagaagaa gctggtgaat accgtaatgt tgaagaaagc 360 ttggaacgtg ttcttgttat ctaccgttac ttgtcagaac ttttccaaaa aggtttggat 420 gtcactgatg aagaaggtga cgatgtgaca aacggtatct ttgtaggcgc taaaactgaa 480 acagataaaa caatttggat gcttgcagcc gaacttggac aagcacctgg tttg 534 <210> 152 <211> 178 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (178) <223> peroxide resistance protein <400> 152 Met Asn Glu Val Lys Lys Met Val Glu Leu Lys Lys Glu Ala Val Lys 1 5 10 15 Asp Val Thr Ser Leu Thr Lys Ala Ala Pro Val Ala Leu Ala Lys Thr 20 25 30 Lys Glu Val Leu Asn Gln Ala Val Ala Asp Leu Tyr Val Ala His Val 35 40 45 Ala Leu His Gln Val His Trp Tyr Met His Gly Arg Gly Phe Leu Val 50 55 60 Trp His Pro Lys Met Asp Glu Tyr Met Glu Ala Leu Asp Gly Gln Leu 65 70 75 80 Asp Glu Ile Ser Glu Arg Leu Ile Thr Leu Gly Gly Ser Pro Phe Ser 85 90 95 Thr Leu Thr Glu Phe Leu Gln Asn Ser Glu Ile Glu Glu Glu Ala Gly 100 105 110 Glu Tyr Arg Asn Val Glu Glu Ser Leu Glu Arg Val Leu Val Ile Tyr 115 120 125 Arg Tyr Leu Ser Glu Leu Phe Gln Lys Gly Leu Asp Val Thr Asp Glu 130 135 140 Glu Gly Asp Asp Val Thr Asn Gly Ile Phe Val Gly Ala Lys Thr Glu 145 150 155 160 Thr Asp Lys Thr Ile Trp Met Leu Ala Ala Glu Leu Gly Gln Ala Pro 165 170 175 Gly leu <210> 153 <211> 777 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (777) <223> Streptococcus pneumoniae spr1432 gene <400> 153 ttgaaaaaag aggtatgtga aatgtcacgt aaaccattta tcgctggtaa ctggaaaatg 60 aacaaaaatc cagaagaagc taaagcattc gttgaagcag ttgcatcaaa acttccttca 120 tcagatcttg ttgaagcagg tatcgctgct ccagctcttg atttgacaac tgttcttgct 180 gttgcaaaag gctcaaacct taaagttgct gctcaaaact gctactttga aaatgcaggt 240 gctttcactg gtgaaactag cccacaagtt ttgaaagaaa tcggtactga ctacgttgtt 300 atcggtcact cagaacgccg tgactacttc catgaaactg acgaagatat caacaaaaaa 360 gcaaaagcaa tctttgcgaa cggtatgctt ccaatcatct gttgtggtga atcacttgaa 420 acttacgaag ctggtaaagc tgctgaattc gtaggtgctc aagtatctgc tgcattggct 480 ggattgactg ctgaacaagt tgctgcctca gttatcgctt atgagccaat ctgggctatc 540 ggtactggta aatcagcttc acaagacgat gcacaaaaaa tgtgtaaagt tgttcgtgac 600 gttgtagctg ctgactttgg tcaagaagtc gcagacaaag ttcgtgttca atacggtggt 660 tctgttaaac ctgaaaatgt tgcttcatac atggcttgcc cagacgttga cggtgccctt 720 gtaggtggtg cgtcacttga agctgaaagc ttcttggctt tgcttgactt tgtaaaa 777 <210> 154 <211> 259 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (259) <223> Triose phosphate isomerase <400> 154 Met Lys Lys Glu Val Cys Glu Met Ser Arg Lys Pro Phe Ile Ala Gly 1 5 10 15 Asn Trp Lys Met Asn Lys Asn Pro Glu Glu Ala Lys Ala Phe Val Glu 20 25 30 Ala Val Ala Ser Lys Leu Pro Ser Ser Asp Leu Val Glu Ala Gly Ile 35 40 45 Ala Ala Pro Ala Leu Asp Leu Thr Thr Val Val Leu Ala Val Ala Lys Gly 50 55 60 Ser Asn Leu Lys Val Ala Ala Gln Asn Cys Tyr Phe Glu Asn Ala Gly 65 70 75 80 Ala Phe Thr Gly Glu Thr Ser Pro Gln Val Leu Lys Glu Ile Gly Thr 85 90 95 Asp Tyr Val Val Ile Gly His Ser Glu Arg Arg Asp Tyr Phe His Glu 100 105 110 Thr Asp Glu Asp Ile Asn Lys Lys Ala Lys Ala Ile Phe Ala Asn Gly 115 120 125 Met Leu Pro Ile Ile Cys Cys Gly Glu Ser Leu Glu Thr Tyr Glu Ala 130 135 140 Gly Lys Ala Ala Glu Phe Val Gly Ala Gln Val Ser Ala Ala Leu Ala 145 150 155 160 Gly Leu Thr Ala Glu Gln Val Ala Ala Ser Val Ile Ala Tyr Glu Pro 165 170 175 Ile Trp Ala Ile Gly Thr Gly Lys Ser Ala Ser Gln Asp Asp Ala Gln 180 185 190 Lys Met Cys Lys Val Val Arg Asp Val Val Ala Ala Asp Phe Gly Gln 195 200 205 Glu Val Ala Asp Lys Val Arg Val Gln Tyr Gly Gly Ser Val Lys Pro 210 215 220 Glu Asn Val Ala Ser Tyr Met Ala Cys Pro Asp Val Asp Gly Ala Leu 225 230 235 240 Val Gly Gly Ala Ser Leu Glu Ala Glu Ser Phe Leu Ala Leu Leu Asp 245 250 255 Phe Val Lys <210> 155 <211> 675 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (675) <223> Streptococcus pneumoniae spr1433 gene <400> 155 atgacatatt tagacgcttt taaatcaggt accttggttt taccgagtgc cctgctcttg 60 cattttaagg aactctttcc ttctagcgac gattttctgg tttggcaatt tttctatttg 120 caaaatacga caggcttaga agaaatgtcg ccaagccaga ttgctgaaag gattggcaag 180 gaaatttcgg atgtcaacca gtccatttct aatctgacgg aaaggggact gctccagtat 240 cgtactatcg aattaaatgg cgaaattgaa ttgctctttg atgctagttt ggccttggaa 300 cgtttggatg acctgtttgg agcagttcat tcaagttcag accagctaac atctcaaaac 360 cagctcaagg atttggtgga aaccttccag caggagttgg gacgattgtt gacgcctttt 420 gagattgagg atttgaccaa gacactaaag gaagatggaa ccagtgctga cttgattaag 480 gaggctcttc gtgaagctgt tttgaatgga aaaccaaact ggaagtacat tcaggcgatt 540 ttgagaaact ggcgccatga aggaatcaag agtgtggctc aaattgaggc caagagagca 600 gaaagagaag caagcaatcc tcagttgaca caggtatctg cagatttcag aaatgccatg 660 gatctctgga aggat 675 <210> 156 <211> 225 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (225) <223> Conserved hypothetical protein <400> 156 Met Thr Tyr Leu Asp Ala Phe Lys Ser Gly Thr Leu Val Leu Pro Ser 1 5 10 15 Ala Leu Leu Leu His Phe Lys Glu Leu Phe Pro Ser Ser Asp Asp Phe 20 25 30 Leu Val Trp Gln Phe Phe Tyr Leu Gln Asn Thr Thr Gly Leu Glu Glu 35 40 45 Met Ser Pro Ser Gln Ile Ala Glu Arg Ile Gly Lys Glu Ile Ser Asp 50 55 60 Val Asn Gln Ser Ile Ser Asn Leu Thr Glu Arg Gly Leu Leu Gln Tyr 65 70 75 80 Arg Thr Ile Glu Leu Asn Gly Glu Ile Glu Leu Leu Phe Asp Ala Ser 85 90 95 Leu Ala Leu Glu Arg Leu Asp Asp Leu Phe Gly Ala Val His Ser Ser 100 105 110 Ser Asp Gln Leu Thr Ser Gln Asn Gln Leu Lys Asp Leu Val Glu Thr 115 120 125 Phe Gln Gln Glu Leu Gly Arg Leu Leu Thr Pro Phe Glu Ile Glu Asp 130 135 140 Leu Thr Lys Thr Leu Lys Glu Asp Gly Thr Ser Ala Asp Leu Ile Lys 145 150 155 160 Glu Ala Leu Arg Glu Ala Val Leu Asn Gly Lys Pro Asn Trp Lys Tyr 165 170 175 Ile Gln Ala Ile Leu Arg Asn Trp Arg His Glu Gly Ile Lys Ser Val 180 185 190 Ala Gln Ile Glu Ala Lys Arg Ala Glu Arg Glu Ala Ser Asn Pro Gln 195 200 205 Leu Thr Gln Val Ser Ala Asp Phe Arg Asn Ala Met Asp Leu Trp Lys 210 215 220 Asp 225 <210> 157 <211> 942 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (942) <223> Streptococcus pneumoniae spr1434 gene <400> 157 atgccgattc gaattgataa aaaattgcca gctgttgaga ttttacggac agagaatatc 60 tttgtcatgg atgatcaacg tgctgcccac caagatatcc gtcctttgaa gattttaatt 120 ttaaatctca tgccacagaa aatggtcaca gagacccagt tgttgcgcca cttggctaat 180 acacccctac aactggatat tgattttctc tatatggaga gccaccgttc taaaacaact 240 cgttcagagc acatggagac cttctataaa acttttcctg aagtcaagga tgagtatttt 300 gatgggatga tcatcacggg tgctccagtt gagcatttac catttgagga agtggactat 360 tgggaggaat ttagacagat gcttgagtgg tctaagactc atgtctattc gacccttcat 420 atctgttggg gggctcaggc tgggctttat ctgcgctatg gtgtagaaaa ataccagatg 480 gacagtaagc tatcaggtat ttatcctcag gacaccctaa aagagggtca ccttctattt 540 agaggctttg atgatagcta tgtatcccct cattcacggc acacggagat ttctaaggaa 600 gaggtcttaa acaagaccaa tctcgagatt ttatcagaag gacctcaggt tggggtttct 660 attttggcca gtcgtgattt acgagaaatt tatagttttg gtcatttgga gtatgaccgt 720 gataccttgg caaaagagta ttttcgagat cgtgatgcag gtttcgaccc acatattcca 780 gaaaattact ttaaagatga tgatgttaat caggtacctt gtctttgttg gtcttcatct 840 gcagccctct ttttcagtaa ttgggtaaac catgcggtct atcaggagac gccttttgat 900 tggagaaaga tagaagatga tgcatctgca tatgggtatt ta 942 <210> 158 <211> 314 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (314) <223> Homoserine O-succinyltransferase <400> 158 Met Pro Ile Arg Ile Asp Lys Lys Leu Pro Ala Val Glu Ile Leu Arg 1 5 10 15 Thr Glu Asn Ile Phe Val Met Asp Asp Gln Arg Ala Ala His Gln Asp 20 25 30 Ile Arg Pro Leu Lys Ile Leu Ile Leu Asn Leu Met Pro Gln Lys Met 35 40 45 Val Thr Glu Thr Gln Leu Leu Arg His Leu Ala Asn Thr Pro Leu Gln 50 55 60 Leu Asp Ile Asp Phe Leu Tyr Met Glu Ser His Arg Ser Lys Thr Thr 65 70 75 80 Arg Ser Glu His Met Glu Thr Phe Tyr Lys Thr Phe Pro Glu Val Lys 85 90 95 Asp Glu Tyr Phe Asp Gly Met Ile Ile Thr Gly Ala Pro Val Glu His 100 105 110 Leu Pro Phe Glu Glu Val Asp Tyr Trp Glu Glu Phe Arg Gln Met Leu 115 120 125 Glu Trp Ser Lys Thr His Val Tyr Ser Thr Leu His Ile Cys Trp Gly 130 135 140 Ala Gln Ala Gly Leu Tyr Leu Arg Tyr Gly Val Glu Lys Tyr Gln Met 145 150 155 160 Asp Ser Lys Leu Ser Gly Ile Tyr Pro Gln Asp Thr Leu Lys Glu Gly 165 170 175 His Leu Leu Phe Arg Gly Phe Asp Asp Ser Tyr Val Ser Pro His Ser 180 185 190 Arg His Thr Glu Ile Ser Lys Glu Glu Val Leu Asn Lys Thr Asn Leu 195 200 205 Glu Ile Leu Ser Glu Gly Pro Gln Val Gly Val Ser Ile Leu Ala Ser 210 215 220 Arg Asp Leu Arg Glu Ile Tyr Ser Phe Gly His Leu Glu Tyr Asp Arg 225 230 235 240 Asp Thr Leu Ala Lys Glu Tyr Phe Arg Asp Arg Asp Ala Gly Phe Asp 245 250 255 Pro His Ile Pro Glu Asn Tyr Phe Lys Asp Asp Asp Val Asn Gln Val 260 265 270 Pro Cys Leu Cys Trp Ser Ser Ser Ala Ala Leu Phe Phe Ser Asn Trp 275 280 285 Val Asn His Ala Val Tyr Gln Glu Thr Pro Phe Asp Trp Arg Lys Ile 290 295 300 Glu Asp Asp Ala Ser Ala Tyr Gly Tyr Leu 305 310 <210> 159 <211> 786 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (786) <223> Streptococcus pneumoniae spr1439 gene <400> 159 atggcacatt tattagaaaa aactagaaaa attacatcaa ttttgaaacg ctcagaggag 60 cagttgcagg atgagctccc ttacaacgct attacgcgtc agttagcgga tattattcat 120 tgcaatgcct gcattatcaa tagtaaggga cgtctgcttg gctattttat gcgttataaa 180 acaaatacag atcgcgtaga gcaattcttc caaactaaga ttttcccaga tgactacgtt 240 caaggggcta atatgattta cgaaacagaa gcaaacttgc ctgttgagca tgatatgagt 300 attttccctg ttgagagtag agatgatttt ccagatggct tgacgactat tgcaccgatt 360 catgtatcgg ggattcgcct tggttctttg attatttggc gtaatgataa aaaattcgaa 420 gatgaggact tggttcttgt tgagattgcc agtaccgttg ttgggattca gcttcttaac 480 ttccaacgtg aagaagatga gaaaaatatt cgtcgtcgta ctgctgtcac catggcggtc 540 aatacccttt cttactccga actccgtgct gtttcagcaa ttttagggga attaaatgga 600 aatgaagggc agttgactgc gtcagtgatt gcagatcgta tcggaatcac tcgctctgtg 660 attgtcaatg ctcttcgtaa acttgagtct gcggggatta ttgaaagtcg ctcacttgga 720 atgaagggaa cctatcttaa ggtcttgatt tcagatattt ttgaagaagt gaagaaaaga 780 gattac 786 <210> 160 <211> 262 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (262) <223> Transcriptional pleiotropic repressor <400> 160 Met Ala His Leu Leu Glu Lys Thr Arg Lys Ile Thr Ser Ile Leu Lys 1 5 10 15 Arg Ser Glu Glu Gln Leu Gln Asp Glu Leu Pro Tyr Asn Ala Ile Thr 20 25 30 Arg Gln Leu Ala Asp Ile Ile His Cys Asn Ala Cys Ile Ile Asn Ser 35 40 45 Lys Gly Arg Leu Leu Gly Tyr Phe Met Arg Tyr Lys Thr Asn Thr Asp 50 55 60 Arg Val Glu Gln Phe Phe Gln Thr Lys Ile Phe Pro Asp Asp Tyr Val 65 70 75 80 Gln Gly Ala Asn Met Ile Tyr Glu Thr Glu Ala Asn Leu Pro Val Glu 85 90 95 His Asp Met Ser Ile Phe Pro Val Glu Ser Arg Asp Asp Phe Pro Asp 100 105 110 Gly Leu Thr Thr Ile Ala Pro Ile His Val Ser Gly Ile Arg Leu Gly 115 120 125 Ser Leu Ile Ile Trp Arg Asn Asp Lys Lys Phe Glu Asp Glu Asp Leu 130 135 140 Val Leu Val Glu Ile Ala Ser Thr Val Val Gly Ile Gln Leu Leu Asn 145 150 155 160 Phe Gln Arg Glu Glu Asp Glu Lys Asn Ile Arg Arg Arg Thr Ala Val 165 170 175 Thr Met Ala Val Asn Thr Leu Ser Tyr Ser Glu Leu Arg Ala Val Ser 180 185 190 Ala Ile Leu Gly Glu Leu Asn Gly Asn Glu Gly Gln Leu Thr Ala Ser 195 200 205 Val Ile Ala Asp Arg Ile Gly Ile Thr Arg Ser Val Ile Val Asn Ala 210 215 220 Leu Arg Lys Leu Glu Ser Ala Gly Ile Ile Glu Ser Arg Ser Leu Gly 225 230 235 240 Met Lys Gly Thr Tyr Leu Lys Val Leu Ile Ser Asp Ile Phe Glu Glu 245 250 255 Val Lys Lys Arg Asp Tyr 260 <210> 161 <211> 276 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (276) <223> Streptococcus pneumoniae spr1458 gene <400> 161 ttgccacggt tcttttgcca taccttactc cttgtttttt tttacttttc ttattacaat 60 ataaatatga acatgaaaat cacacttata cctgaacgat gtatcgcctg tggactttgc 120 caaacttatt ctgatttatt tgattaccac gataatggaa tcgtgcgttt ttacgatgac 180 cctgaccaac tggaaaaaga aatttctcct agtcaggatg tcttagaggc tgttaaaaat 240 tgcccaactc gcgccctgat tggaaaccag gaagcc 276 <210> 162 <211> 92 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (92) <223> Ferredoxin <400> 162 Met Pro Arg Phe Phe Cys His Thr Leu Leu Leu Val Phe Phe Tyr Phe 1 5 10 15 Ser Tyr Tyr Asn Ile Asn Met Asn Met Lys Ile Thr Leu Ile Pro Glu 20 25 30 Arg Cys Ile Ala Cys Gly Leu Cys Gln Thr Tyr Ser Asp Leu Phe Asp 35 40 45 Tyr His Asp Asn Gly Ile Val Arg Phe Tyr Asp Asp Pro Asp Gln Leu 50 55 60 Glu Lys Glu Ile Ser Pro Ser Gln Asp Val Leu Glu Ala Val Lys Asn 65 70 75 80 Cys Pro Thr Arg Ala Leu Ile Gly Asn Gln Glu Ala 85 90 <210> 163 <211> 1017 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene <222> (1) .. (1017) <223> Streptococcus pneumoniae spr1460 gene <400> 163 atgcaagaaa agattttggt aactggtggt gccggtttta ttggaaccca cactgttatt 60 gaattgatcc aagcaggtca tcaagttgtt gtggtggata atcttgtcaa tagcaatcgt 120 aagagtttag aagttgttga aagaatcaca ggagttgaga ttcctttcta tgaggcagat 180 attcgtgaca cagataccct cagagatatt ttcaagcagg aagaaccgac tggtgtcatt 240 cactttgctg gtttgaaggc tgttggcgaa tcaacacgta tccctcttgc ctactatgac 300 aacaatatcg ctggaactgt cagccttttg aaagccatgg aagaaaacaa ctgtaaaaac 360 atcatcttca gttcttctgc gacagtttac ggggatccgc acacagtgcc catcttggaa 420 gatttcccac tttcagtgac caacccatac ggtcgtacta agctcatgct agaggaaatt 480 ttgactgata tttacaaagc agactcagaa tggaatgttg tcttgcttcg ttactttaac 540 ccaatcggag cccatgagag tggtgatttg ggagaaaatc caaacggtat tccaaacaat 600 ctcttgccat atgtgactca agtagccgtt ggaaaattag agcaagtgca agtgtttgga 660 gacgattacg atacggaaga tggaacaggt gttcgtgact atatccacgt tgtcgatttg 720 gctaagggtc acgttgcagc tttgaaaaaa atccaaaaag gttcaggact aaacgtttat 780 aaccttggaa ctggtaaagg ttactcagtt cttgaaatta tccaaaacat ggaaaaagcg 840 gtgggatgtc ctattcctta ccgcatcgta gaacgtcgcc caggtgatat cgctgcctgc 900 tactcagacc cagcaaaggc taaagcagaa ctcggttggg aagcagaact cgacatcacc 960 caaatgtgtg aagacgcatg gcgttggcag agcaagcatc caaatggatt tgaagac 1017 <210> 164 <211> 339 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (339) UDP-glucose 4-epimerase <400> 164 Met Gln Glu Lys Ile Leu Val Thr Gly Gly Ala Gly Phe Ile Gly Thr 1 5 10 15 His Thr Val Ile Glu Leu Ile Gln Ala Gly His Gln Val Val Val Val 20 25 30 Asp Asn Leu Val Asn Ser Asn Arg Lys Ser Leu Glu Val Val Glu Arg 35 40 45 Ile Thr Gly Val Glu Ile Pro Phe Tyr Glu Ala Asp Ile Arg Asp Thr 50 55 60 Asp Thr Leu Arg Asp Ile Phe Lys Gln Glu Glu Pro Thr Gly Val Ile 65 70 75 80 His Phe Ala Gly Leu Lys Ala Val Gly Glu Ser Thr Arg Ile Pro Leu 85 90 95 Ala Tyr Tyr Asp Asn Asn Ile Ala Gly Thr Val Ser Leu Leu Lys Ala 100 105 110 Met Glu Glu Asn Asn Cys Lys Asn Ile Ile Phe Ser Ser Ser Ala Thr 115 120 125 Val Tyr Gly Asp Pro His Thr Val Pro Ile Leu Glu Asp Phe Pro Leu 130 135 140 Ser Val Thr Asn Pro Tyr Gly Arg Thr Lys Leu Met Leu Glu Glu Ile 145 150 155 160 Leu Thr Asp Ile Tyr Lys Ala Asp Ser Glu Trp Asn Val Val Leu Leu 165 170 175 Arg Tyr Phe Asn Pro Ile Gly Ala His Glu Ser Gly Asp Leu Gly Glu 180 185 190 Asn Pro Asn Gly Ile Pro Asn Asn Leu Leu Pro Tyr Val Thr Gln Val 195 200 205 Ala Val Gly Lys Leu Glu Gln Val Gln Val Phe Gly Asp Asp Tyr Asp 210 215 220 Thr Glu Asp Gly Thr Gly Val Arg Asp Tyr Ile His Val Val Asp Leu 225 230 235 240 Ala Lys Gly His Val Ala Ala Leu Lys Lys Ile Gln Lys Gly Ser Gly 245 250 255 Leu Asn Val Tyr Asn Leu Gly Thr Gly Lys Gly Tyr Ser Val Leu Glu 260 265 270 Ile Ile Gln Asn Met Glu Lys Ala Val Gly Cys Pro Ile Pro Tyr Arg 275 280 285 Ile Val Glu Arg Arg Pro Gly Asp Ile Ala Ala Cys Tyr Ser Asp Pro 290 295 300 Ala Lys Ala Lys Ala Glu Leu Gly Trp Glu Ala Glu Leu Asp Ile Thr 305 310 315 320 Gln Met Cys Glu Asp Ala Trp Arg Trp Gln Ser Lys His Pro Asn Gly 325 330 335 Phe Glu Asp <210> 165 <211> 627 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene <222> (1) .. (627) <223> Streptococcus pneumoniae spr1468 gene <400> 165 atgaaactag cagttattgc agcaaatgga caagtgggga aagcaattgt tgaagaagca 60 gtcaaacgag gacatgaagt aacggctatt gttcgttctg aaaacaaaag tcaagctgag 120 tctattatta aaaaagattt atttgagttg acaaaagatg atcttactgg atttgatgct 180 gttatttcag cgtttggtgc ttatacacca gatacgctac cgctccatag caaatcaatt 240 gaactattta atcagcttct tgctggaaca cagacacgtt ttctagttgt agggggagca 300 ggaagtcttt atatagatga aacaaaaacg acacgacttt tagatacgcc tgattttcct 360 gaagaattta agtcactagc aaaagctcaa gcggatgaat tggatcttct tcgtacaaaa 420 aataatctca actggacttt tgtcagtcct gctgtagatt ttattcccga tggtgaaaaa 480 acaggaaact acattctagc aggagaaatt tttactacta atgaaaaagg aataagccag 540 attagttatg cagattatgc tattggattg gtagatgaac ttgaaaaagg tcatcatata 600 aaagaacgta tctctttact tgaaaaa 627 <210> 166 <211> 209 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (209) <223> Conserved hypothetical protein <400> 166 Met Lys Leu Ala Val Ile Ala Ala Asn Gly Gln Val Gly Lys Ala Ile 1 5 10 15 Val Glu Glu Ala Val Lys Arg Gly His Glu Val Thr Ala Ile Val Arg 20 25 30 Ser Glu Asn Lys Ser Gln Ala Glu Ser Ile Ile Lys Lys Asp Leu Phe 35 40 45 Glu Leu Thr Lys Asp Asp Leu Thr Gly Phe Asp Ala Val Ile Ser Ala 50 55 60 Phe Gly Ala Tyr Thr Pro Asp Thr Leu Pro Leu His Ser Lys Ser Ile 65 70 75 80 Glu Leu Phe Asn Gln Leu Leu Ala Gly Thr Gln Thr Arg Phe Leu Val 85 90 95 Val Gly Gly Ala Gly Ser Leu Tyr Ile Asp Glu Thr Lys Thr Thr Arg 100 105 110 Leu Leu Asp Thr Pro Asp Phe Pro Glu Glu Phe Lys Ser Leu Ala Lys 115 120 125 Ala Gln Ala Asp Glu Leu Asp Leu Leu Arg Thr Lys Asn Asn Leu Asn 130 135 140 Trp Thr Phe Val Ser Pro Ala Val Asp Phe Ile Pro Asp Gly Glu Lys 145 150 155 160 Thr Gly Asn Tyr Ile Leu Ala Gly Glu Ile Phe Thr Thr Asn Glu Lys 165 170 175 Gly Ile Ser Gln Ile Ser Tyr Ala Asp Tyr Ala Ile Gly Leu Val Asp 180 185 190 Glu Leu Glu Lys Gly His His Ile Lys Glu Arg Ile Ser Leu Leu Glu 195 200 205 Lys <210> 167 <211> 1980 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1980) <223> Streptococcus pneumoniae spr1472 gene <400> 167 atgcttagaa actatgaaat aataaaaaag gagaacatca tgattaacat tactttccca 60 gatggcgctg ttcgtgaatt cgaatctggc gtaacaactt ttgaaattgc ccaatctatc 120 agcaattccc tagctaaaaa agccttggct ggtaaattca acggcaaact catcgacact 180 actcgcgcta tcactgaaga tggaagcatc gaaattgtga cacctgatca cgaagatgcc 240 cttccaatct tgcgtcactc agcaactcac ttgttcgccc aagcagctcg tcgtcttttc 300 ccagacattc acttgggagt tggtccagcc atcgaagatg gtttctacta cgatactgac 360 cacacagctg gtcaaatctc taacgaagac cttcctcgta tcgaagaaga aatgcaaaaa 420 atcgtcaaag aaaacttccc atctattcgt gaagaagtga ctaaagacga ggcacgtgaa 480 atcttcaaaa atgaccctta caagttggaa ttgattgaag aacactcaga agacgaaggc 540 ggtttgacta tctatcgtca gggtgaatat gtagacctct gccgtggacc tcacgttcca 600 tcaacaggtc gtatccaaat cttccacctt ctccatgtag ctggtgcgta ctggcgtgga 660 aacagcgaca acgctatgat gcaacgtatc tacggtacag cttggtttga caagaaagac 720 ttgaaaaact accttcaaat gcgtgaagaa gctaaggaac gtgaccaccg taaacttggt 780 aaagagcttg acctctttat gatttcacaa gaagtgggac aaggtttgcc attctggttg 840 ccaaatggtg cgactatccg tcgtgaattg gaacgctaca tcgtaaacaa agagttggtt 900 tctggctacc aacacgtcta cactccacca cttgcttctg ttgagcttta caagacttct 960 ggtcactggg atcattacca agaagacatg ttcccaacca tggacatggg tgacggggaa 1020 gaatttgtcc ttcgtccaat gaactgtccg caccacatcc aagttttcaa acaccatgtt 1080 cactcttacc gtgaattgcc aatccgtatc gctgaaatcg gtatgatgca ccgttacgaa 1140 aaatctggtg ccctcactgg ccttcaacgt gtacgtgaaa tgtcactcaa cgacggtcac 1200 ctattcgtta ctccagaaca aatccaagaa gaattccaac gtgcccttca gttgattatc 1260 gatgtttatg aagacttcaa cttgactgac taccgcttcc gcctctctct tcgtgaccct 1320 caagatactc ataagtactt tgataacgat gagatgtggg aaaatgccca aaccatgctt 1380 cgtgcagctc ttgatgaaat gggcgtggac tactttgaag ccgaaggtga agcagccttc 1440 tacggaccaa aattggatat ccagattaaa actgcccttg gaaaagaaga aaccctttct 1500 actatccaac ttgatttctt gttgccagaa cgcttcgacc tcaaatacat cggagctgat 1560 ggcgaagatc accgtccagt catgatccac cgtggggtta tctcaactat ggaacgcttc 1620 acagctatct tgattgagaa ctacaagggg gccttcccaa catggctggc accacaccaa 1680 gtaaccctca tcccagtatc taacgaaaaa cacgtggact acgcttggga agtggccaag 1740 aaactccgtg accgcggtgt ccgtgcagac gtagatgagc gcaatgaaaa aatgcagttc 1800 aagatccgtg cttcacaaac cagcaagatt ccttaccaat taattgttgg agacaaagaa 1860 atggaagacg aaacagtcaa cgttcgtcgc tacggccaaa aagaaacaca aactgtctca 1920 gttgataatt ttgttcaagc tatcctagct gatatcgcca acaaatcacg cgttgagaaa 1980 1980 <210> 168 <211> 660 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (660) Threonyl-tRNA synthetase 1 <400> 168 Met Leu Arg Asn Tyr Glu Ile Ile Lys Lys Glu Asn Ile Met Ile Asn 1 5 10 15 Ile Thr Phe Pro Asp Gly Ala Val Arg Glu Phe Glu Ser Gly Val Thr 20 25 30 Thr Phe Glu Ile Ala Gln Ser Ile Ser Asn Ser Leu Ala Lys Lys Ala 35 40 45 Leu Ala Gly Lys Phe Asn Gly Lys Leu Ile Asp Thr Thr Arg Ala Ile 50 55 60 Thr Glu Asp Gly Ser Ile Glu Ile Val Thr Pro Asp His Glu Asp Ala 65 70 75 80 Leu Pro Ile Leu Arg His Ser Ala Thr His Leu Phe Ala Gln Ala Ala 85 90 95 Arg Arg Leu Phe Pro Asp Ile His Leu Gly Val Gly Pro Ala Ile Glu 100 105 110 Asp Gly Phe Tyr Tyr Asp Thr Asp His Thr Ala Gly Gln Ile Ser Asn 115 120 125 Glu Asp Leu Pro Arg Ile Glu Glu Glu Met Gln Lys Ile Val Lys Glu 130 135 140 Asn Phe Pro Ser Ile Arg Glu Glu Val Thr Lys Asp Glu Ala Arg Glu 145 150 155 160 Ile Phe Lys Asn Asp Pro Tyr Lys Leu Glu Leu Ile Glu Glu His Ser 165 170 175 Glu Asp Glu Gly Gly Leu Thr Ile Tyr Arg Gln Gly Glu Tyr Val Asp 180 185 190 Leu Cys Arg Gly Pro His Val Pro Ser Thr Gly Arg Ile Gln Ile Phe 195 200 205 His Leu Leu His Val Ala Gly Ala Tyr Trp Arg Gly Asn Ser Asp Asn 210 215 220 Ala Met Met Gln Arg Ile Tyr Gly Thr Ala Trp Phe Asp Lys Lys Asp 225 230 235 240 Leu Lys Asn Tyr Leu Gln Met Arg Glu Glu Ala Lys Glu Arg Asp His 245 250 255 Arg Lys Leu Gly Lys Glu Leu Asp Leu Phe Met Ile Ser Gln Glu Val 260 265 270 Gly Gln Gly Leu Pro Phe Trp Leu Pro Asn Gly Ala Thr Ile Arg Arg 275 280 285 Glu Leu Glu Arg Tyr Ile Val Asn Lys Glu Leu Val Ser Gly Tyr Gln 290 295 300 His Val Tyr Thr Pro Pro Leu Ala Ser Val Glu Leu Tyr Lys Thr Ser 305 310 315 320 Gly His Trp Asp His Tyr Gln Glu Asp Met Phe Pro Thr Met Asp Met 325 330 335 Gly Asp Gly Glu Glu Phe Val Leu Arg Pro Met Asn Cys Pro His His 340 345 350 Ile Gln Val Phe Lys His His Val His Ser Tyr Arg Glu Leu Pro Ile 355 360 365 Arg Ile Ala Glu Ile Gly Met Met His Arg Tyr Glu Lys Ser Gly Ala 370 375 380 Leu Thr Gly Leu Gln Arg Val Arg Glu Met Ser Leu Asn Asp Gly His 385 390 395 400 Leu Phe Val Thr Pro Glu Gln Ile Gln Glu Glu Phe Gln Arg Ala Leu 405 410 415 Gln Leu Ile Ile Asp Val Tyr Glu Asp Phe Asn Leu Thr Asp Tyr Arg 420 425 430 Phe Arg Leu Ser Leu Arg Asp Pro Gln Asp Thr His Lys Tyr Phe Asp 435 440 445 Asn Asp Glu Met Trp Glu Asn Ala Gln Thr Met Leu Arg Ala Ala Leu 450 455 460 Asp Glu Met Gly Val Asp Tyr Phe Glu Ala Glu Gly Glu Ala Ala Phe 465 470 475 480 Tyr Gly Pro Lys Leu Asp Ile Gln Ile Lys Thr Ala Leu Gly Lys Glu 485 490 495 Glu Thr Leu Ser Thr Ile Gln Leu Asp Phe Leu Leu Pro Glu Arg Phe 500 505 510 Asp Leu Lys Tyr Ile Gly Ala Asp Gly Glu Asp His Arg Pro Val Met 515 520 525 Ile His Arg Gly Val Ile Ser Thr Met Glu Arg Phe Thr Ala Ile Leu 530 535 540 Ile Glu Asn Tyr Lys Gly Ala Phe Pro Thr Trp Leu Ala Pro His Gln 545 550 555 560 Val Thr Leu Ile Pro Val Ser Asn Glu Lys His Val Asp Tyr Ala Trp 565 570 575 Glu Val Ala Lys Lys Leu Arg Asp Arg Gly Val Arg Ala Asp Val Asp 580 585 590 Glu Arg Asn Glu Lys Met Gln Phe Lys Ile Arg Ala Ser Gln Thr Ser 595 600 605 Lys Ile Pro Tyr Gln Leu Ile Val Gly Asp Lys Glu Met Glu Asp Glu 610 615 620 Thr Val Asn Val Arg Arg Tyr Gly Gln Lys Glu Thr Gln Thr Val Ser 625 630 635 640 Val Asp Asn Phe Val Gln Ala Ile Leu Ala Asp Ile Ala Asn Lys Ser 645 650 655 Arg Val Glu Lys 660 <210> 169 <211> 2790 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (2790) <223> Streptococcus pneumoniae spr1502 gene <400> 169 atgaaactca aagacaccct taatcttggg aaaactgaat tcccaatgcg tgcaggcctt 60 cctaccaaag agccagtttg gcaaaaggaa tgggaatatg caaaacttta tcaacgtcgt 120 caagaattga accaaggaaa acctcatttc accttgcatg atggccctcc atacgctaac 180 ggaaatatcc acgttggaca tgctatgaac aagatttcaa aagatatcat tgttcgttct 240 aagtctatgt caggatttta cgcaccattt attcctggtt gggatactca tggtctgcca 300 atcgagcaag tcttgtcaaa acaaggtgtc aaacgtaaag aaatggactt ggttgagtac 360 ttgaaacttt gccgtgagta cgctctttct caagtagata aacaacgtga agattttaaa 420 cgtttgggtg tttctggtga ctgggaaaat ccatatgtga ccttgactcc tgactatgaa 480 gcagctcaaa ttcgtgtatt tggtgagatg gctaataagg gttatatcta ccgtggtgct 540 aagccagttt actggtcatg gtcatctgag tcagcccttg ctgaagcaga gattgaatac 600 catgacttgg tttcaacttc cctttactat gccaacaagg taaaagatgg caaaggagtt 660 ctagatacag atacttatat cgttgtctgg acaacgactc catttactat cacagcctct 720 cgtggtttga cggttggtgc agatattgat tacgttttgg ttcaacctgc tggtgaagct 780 cgtaagtttg tcgttgctgc tgaattattg actagcttgt ctgagaaatt tggctgggct 840 gatgttcaag ttttggaaac ttaccgtggc caagaactca accacatcgt aacagaacac 900 ccatgggata cagctgtaga agagttggta attcttggtg accacgttac gactgactct 960 ggtacaggta ttgtccatac agcccctggt tttggtgagg acgattacaa tgttggtatt 1020 gctaataatc ttgaagtcgc agtgactgtt gatgaacgtg gtatcatgat gaagaatgct 1080 ggtcctgaat ttgaaggtca attctatgaa aaggtagttc caactgttat tgaaaaactt 1140 ggtaacctcc ttcttgccca agaagaaatc tctcactcat atccatttga ctggcgtact 1200 aagaaaccaa tcatctggcg tgcagttcca caatggtttg cctcagtttc taaattccgt 1260 caagaaatct tggacgaaat tgaaaaagtg aaattccact cagaatgggg taaagtccgt 1320 ctttacaata tgatccgtga ccgtggtgac tgggttatct ctcgtcaacg tgcttggggt 1380 gttccacttc ctatcttcta cgctgaagat ggtacagcta tcatggtagc tgaaactatt 1440 gaacacgtag ctcaactttt tgaagaacat ggttcaagca tttggtggga acgtgatgcc 1500 aaagacctct tgccagaagg atttactcat ccaggttcac caaacggcga gttcaaaaaa 1560 gaaactgata tcatggacgt ttggtttgac tcaggttcat catggaatgg agtggtggta 1620 aaccgtcctg aattgactta cccagccgac ctttacctag aaggttctga ccaataccgt 1680 ggttggttta actcatcact tatcacatct gttgccaacc atggcgtagc accttacaaa 1740 caaatcttgt cacaaggttt tgcccttgat ggtaaaggtg agaagatgtc taaatctctt 1800 ggaaatacta ttgctccaag cgatgttgaa aaacaattcg gtgctgaaat cttgcgtctc 1860 tgggtaacaa gtgttgactc aagtaatgac gtgcgtatct ctatggatat cttgagccaa 1920 gtttctgaaa cttaccgtaa gattcgtaac actcttcgtt tcttgattgc caatacatct 1980 gactttaacc cagctcaaga tacagtcgct tacgatgagc ttcgttcagt tgataagtac 2040 atgacgattc gctttaacca gcttgtcaag accattcgtg atgcctatgc agactttgaa 2100 ttcttgacga tctacaaggc cttggtgaac tttatcaacg ttgacttgtc agccttctac 2160 cttgattttg ccaaagatgt tgtttacatt gaaggtgcca aatcactgga acgccgtcaa 2220 atgcagactg tcttctatga cattcttgtc aaaatcacca aactcttgac accaatcctt 2280 cctcacactg cggaagaaat ctggtcatat cttgagtttg aaacagaaga cttcgtccaa 2340 ttgtcagaat taccagaagt tcaaactttt gctaaccaag aagaaatctt ggatacatgg 2400 gcagccttca tggactttcg tggacaagca caaaaagcct tggaagaagc tcgtaatgca 2460 aaagttatcg gtaaatcact tgaagcacac ttgacagttt atccaaatga agttgtgaaa 2520 actctactcg aagcagtaaa cagcaatgta gcacaacttt tgatcgtgtc tgagttgacc 2580 atcgcagaag gaccagctcc ggaagctgcc cttagcttcg aagatgtagc cttcacagtt 2640 gaacgtgcta ctggtgaagt atgtgaccgt tgccgtcgta tcgacccaac aacagcagaa 2700 cgcagctacc aggcagttat ctgtgaccac tgtgcaagca tcgtagaaga aaactttgcg 2760 gaagcagtcg cagaaggatt tgaagagaaa 2790 <210> 170 <211> 930 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (930) <223> Isoleucyl-tRNA synthetase <400> 170 Met Lys Leu Lys Asp Thr Leu Asn Leu Gly Lys Thr Glu Phe Pro Met 1 5 10 15 Arg Ala Gly Leu Pro Thr Lys Glu Pro Val Trp Gln Lys Glu Trp Glu 20 25 30 Tyr Ala Lys Leu Tyr Gln Arg Arg Gln Glu Leu Asn Gln Gly Lys Pro 35 40 45 His Phe Thr Leu His Asp Gly Pro Pro Tyr Ala Asn Gly Asn Ile His 50 55 60 Val Gly His Ala Met Asn Lys Ile Ser Lys Asp Ile Ile Val Arg Ser 65 70 75 80 Lys Ser Met Ser Gly Phe Tyr Ala Pro Phe Ile Pro Gly Trp Asp Thr 85 90 95 His Gly Leu Pro Ile Glu Gln Val Leu Ser Lys Gln Gly Val Lys Arg 100 105 110 Lys Glu Met Asp Leu Val Glu Tyr Leu Lys Leu Cys Arg Glu Tyr Ala 115 120 125 Leu Ser Gln Val Asp Lys Gln Arg Glu Asp Phe Lys Arg Leu Gly Val 130 135 140 Ser Gly Asp Trp Glu Asn Pro Tyr Val Thr Leu Thr Pro Asp Tyr Glu 145 150 155 160 Ala Ala Gln Ile Arg Val Phe Gly Glu Met Ala Asn Lys Gly Tyr Ile 165 170 175 Tyr Arg Gly Ala Lys Pro Val Tyr Trp Ser Trp Ser Ser Glu Ser Ala 180 185 190 Leu Ala Glu Ala Glu Ile Glu Tyr His Asp Leu Val Ser Thr Ser Leu 195 200 205 Tyr Tyr Ala Asn Lys Val Lys Asp Gly Lys Gly Val Leu Asp Thr Asp 210 215 220 Thr Tyr Ile Val Val Trp Thr Thr Thr Pro Phe Thr Ile Thr Ala Ser 225 230 235 240 Arg Gly Leu Thr Val Gly Ala Asp Ile Asp Tyr Val Leu Val Gln Pro 245 250 255 Ala Gly Glu Ala Arg Lys Phe Val Val Ala Ala Glu Leu Leu Thr Ser 260 265 270 Leu Ser Glu Lys Phe Gly Trp Ala Asp Val Gln Val Leu Glu Thr Tyr 275 280 285 Arg Gly Gln Glu Leu Asn His Ile Val Thr Glu His Pro Trp Asp Thr 290 295 300 Ala Val Glu Glu Leu Val Ile Leu Gly Asp His Val Thr Thr Asp Ser 305 310 315 320 Gly Thr Gly Ile Val His Thr Ala Pro Gly Phe Gly Glu Asp Asp Tyr 325 330 335 Asn Val Gly Ile Ala Asn Asn Leu Glu Val Ala Val Thr Val Asp Glu 340 345 350 Arg Gly Ile Met Met Lys Asn Ala Gly Pro Glu Phe Glu Gly Gln Phe 355 360 365 Tyr Glu Lys Val Val Pro Thr Val Ile Glu Lys Leu Gly Asn Leu Leu 370 375 380 Leu Ala Gln Glu Glu Ile Ser His Ser Tyr Pro Phe Asp Trp Arg Thr 385 390 395 400 Lys Lys Pro Ile Ile Trp Arg Ala Val Pro Gln Trp Phe Ala Ser Val 405 410 415 Ser Lys Phe Arg Gln Glu Ile Leu Asp Glu Ile Glu Lys Val Lys Phe 420 425 430 His Ser Glu Trp Gly Lys Val Arg Leu Tyr Asn Met Ile Arg Asp Arg 435 440 445 Gly Asp Trp Val Ile Ser Arg Gln Arg Ala Trp Gly Val Pro Leu Pro 450 455 460 Ile Phe Tyr Ala Glu Asp Gly Thr Ala Ile Met Val Ala Glu Thr Ile 465 470 475 480 Glu His Val Ala Gln Leu Phe Glu Glu His Gly Ser Ser Ile Trp Trp 485 490 495 Glu Arg Asp Ala Lys Asp Leu Leu Pro Glu Gly Phe Thr His Pro Gly 500 505 510 Ser Pro Asn Gly Glu Phe Lys Lys Glu Thr Asp Ile Met Asp Val Trp 515 520 525 Phe Asp Ser Gly Ser Ser Trp Asn Gly Val Val Val Asn Arg Pro Glu 530 535 540 Leu Thr Tyr Pro Ala Asp Leu Tyr Leu Glu Gly Ser Asp Gln Tyr Arg 545 550 555 560 Gly Trp Phe Asn Ser Ser Leu Ile Thr Ser Val Ala Asn His Gly Val 565 570 575 Ala Pro Tyr Lys Gln Ile Leu Ser Gln Gly Phe Ala Leu Asp Gly Lys 580 585 590 Gly Glu Lys Met Ser Lys Ser Leu Gly Asn Thr Ile Ala Pro Ser Asp 595 600 605 Val Glu Lys Gln Phe Gly Ala Glu Ile Leu Arg Leu Trp Val Thr Ser 610 615 620 Val Asp Ser Ser Asn Asp Val Arg Ile Ser Met Asp Ile Leu Ser Gln 625 630 635 640 Val Ser Glu Thr Tyr Arg Lys Ile Arg Asn Thr Leu Arg Phe Leu Ile 645 650 655 Ala Asn Thr Ser Asp Phe Asn Pro Ala Gln Asp Thr Val Ala Tyr Asp 660 665 670 Glu Leu Arg Ser Val Asp Lys Tyr Met Thr Ile Arg Phe Asn Gln Leu 675 680 685 Val Lys Thr Ile Arg Asp Ala Tyr Ala Asp Phe Glu Phe Leu Thr Ile 690 695 700 Tyr Lys Ala Leu Val Asn Phe Ile Asn Val Asp Leu Ser Ala Phe Tyr 705 710 715 720 Leu Asp Phe Ala Lys Asp Val Val Tyr Ile Glu Gly Ala Lys Ser Leu 725 730 735 Glu Arg Arg Gln Met Gln Thr Val Phe Tyr Asp Ile Leu Val Lys Ile 740 745 750 Thr Lys Leu Leu Thr Pro Ile Leu Pro His Thr Ala Glu Glu Ile Trp 755 760 765 Ser Tyr Leu Glu Phe Glu Thr Glu Asp Phe Val Gln Leu Ser Glu Leu 770 775 780 Pro Glu Val Gln Thr Phe Ala Asn Gln Glu Glu Ile Leu Asp Thr Trp 785 790 795 800 Ala Ala Phe Met Asp Phe Arg Gly Gln Ala Gln Lys Ala Leu Glu Glu 805 810 815 Ala Arg Asn Ala Lys Val Ile Gly Lys Ser Leu Glu Ala His Leu Thr 820 825 830 Val Tyr Pro Asn Glu Val Val Lys Thr Leu Leu Glu Ala Val Asn Ser 835 840 845 Asn Val Ala Gln Leu Leu Ile Val Ser Glu Leu Thr Ile Ala Glu Gly 850 855 860 Pro Ala Pro Glu Ala Ala Leu Ser Phe Glu Asp Val Ala Phe Thr Val 865 870 875 880 Glu Arg Ala Thr Gly Glu Val Cys Asp Arg Cys Arg Arg Ile Asp Pro 885 890 895 Thr Thr Ala Glu Arg Ser Tyr Gln Ala Val Ile Cys Asp His Cys Ala 900 905 910 Ser Ile Val Glu Glu Asn Phe Ala Glu Ala Val Ala Glu Gly Phe Glu 915 920 925 Glu lys 930 <210> 171 <211> 1257 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1257) <223> Streptococcus pneumoniae spr1510 gene <400> 171 atgacatttt catttgatac agctgctgct caaggggcag tgattaaagt aattggtgtc 60 ggtggaggtg gtggcaatgc catcaaccgt atggtcgacg aaggtgttac aggcgtagaa 120 tttatcgcag caaacacaga tgtacaagca ttgagtagta caaaagctga gactgttatt 180 cagttgggac ctaaattgac tcgtggtttg ggtgcaggag gtcaacctga ggttggtcgt 240 aaagccgctg aagaaagcga agaaacactg acggaagcta ttagtggtgc cgatatggtc 300 ttcatcactg ctggtatggg aggaggctct ggaactggag ctgctcctgt tattgctcgt 360 atcgccaaag atttaggtgc gcttacagtt ggtgttgtaa cacgtccctt tggttttgaa 420 ggaagtaagc gtggacaatt tgctgtagaa ggaatcaatc aacttcgtga gcatgtagac 480 actctattga ttatctcaaa caacaatttg cttgaaattg ttgataagaa aacaccgctt 540 ttggaggctc ttagcgaagc ggataacgtt cttcgtcaag gtgttcaagg gattaccgat 600 ttgattacca atccaggatt gattaacctt gactttgccg atgtgaaaac ggtaatggca 660 aacaaaggga atgctcttat gggtattggt atcggtagtg gagaagaacg tgtggtagaa 720 gcggcacgta aggcaatcta ttcaccactt cttgaaacaa ctattgacgg tgctgaggat 780 gttatcgtca acgttactgg tggtcttgac ttaaccttga ttgaggcaga agaggcttca 840 caaattgtga accaggcagc aggtcaagga gtgaacatct ggctcggtac ttcaattgat 900 gaaagtatgc gtgatgaaat tcgtgtaaca gttgtcgcaa cgggtgttcg tcaagaccgc 960 gtagaaaagg ttgtggctcc acaagctaga tctgctacta actaccgtga gacagtgaaa 1020 ccagctcatt cacatggctt tgatcgtcat tttgatatgg cagaaacagt tgaattgcca 1080 aaacaaaatc cacgtcgttt ggaaccaact caggcatctg cttttggtga ttgggatctt 1140 cgccgtgaat cgattgttcg tacaacagat tcagtcgttt ctccagtcga gcgctttgaa 1200 gccccaattt cacaagatga agatgaattg gatacacctc catttttcaa aaatcgt 1257 <210> 172 <211> 419 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (419) <223> Cell division protein FtsZ <400> 172 Met Thr Phe Ser Phe Asp Thr Ala Ala Ala Gln Gly Ala Val Ile Lys 1 5 10 15 Val Ile Gly Val Gly Gly Gly Gly Gly Asn Ala Ile Asn Arg Met Val 20 25 30 Asp Glu Gly Val Thr Gly Val Glu Phe Ile Ala Ala Asn Thr Asp Val 35 40 45 Gln Ala Leu Ser Ser Thr Lys Ala Glu Thr Val Ile Gln Leu Gly Pro 50 55 60 Lys Leu Thr Arg Gly Leu Gly Ala Gly Gly Gln Pro Glu Val Gly Arg 65 70 75 80 Lys Ala Ala Glu Glu Ser Glu Glu Thr Leu Thr Glu Ala Ile Ser Gly 85 90 95 Ala Asp Met Val Phe Ile Thr Ala Gly Met Gly Gly Gly Ser Gly Thr 100 105 110 Gly Ala Ala Pro Val Ile Ala Arg Ile Ala Lys Asp Leu Gly Ala Leu 115 120 125 Thr Val Gly Val Val Thr Arg Pro Phe Gly Phe Glu Gly Ser Lys Arg 130 135 140 Gly Gln Phe Ala Val Glu Gly Ile Asn Gln Leu Arg Glu His Val Asp 145 150 155 160 Thr Leu Leu Ile Ile Ser Asn Asn Asn Leu Leu Glu Ile Val Asp Lys 165 170 175 Lys Thr Pro Leu Leu Glu Ala Leu Ser Glu Ala Asp Asn Val Leu Arg 180 185 190 Gln Gly Val Gln Gly Ile Thr Asp Leu Ile Thr Asn Pro Gly Leu Ile 195 200 205 Asn Leu Asp Phe Ala Asp Val Lys Thr Val Met Ala Asn Lys Gly Asn 210 215 220 Ala Leu Met Gly Ile Gly Ile Gly Ser Gly Glu Glu Arg Val Val Glu 225 230 235 240 Ala Ala Arg Lys Ala Ile Tyr Ser Pro Leu Leu Glu Thr Thr Ile Asp 245 250 255 Gly Ala Glu Asp Val Ile Val Asn Val Thr Gly Gly Leu Asp Leu Thr 260 265 270 Leu Ile Glu Ala Glu Glu Ala Ser Gln Ile Val Asn Gln Ala Ala Gly 275 280 285 Gln Gly Val Asn Ile Trp Leu Gly Thr Ser Ile Asp Glu Ser Met Arg 290 295 300 Asp Glu Ile Arg Val Thr Val Val Ala Thr Gly Val Arg Gln Asp Arg 305 310 315 320 Val Glu Lys Val Val Ala Pro Gln Ala Arg Ser Ala Thr Asn Tyr Arg 325 330 335 Glu Thr Val Lys Pro Ala His Ser His Gly Phe Asp Arg His Phe Asp 340 345 350 Met Ala Glu Thr Val Glu Leu Pro Lys Gln Asn Pro Arg Arg Leu Glu 355 360 365 Pro Thr Gln Ala Ser Ala Phe Gly Asp Trp Asp Leu Arg Arg Glu Ser 370 375 380 Ile Val Arg Thr Thr Asp Ser Val Val Ser Pro Val Glu Arg Phe Glu 385 390 395 400 Ala Pro Ile Ser Gln Asp Glu Asp Glu Leu Asp Thr Pro Pro Phe Phe 405 410 415 Lys Asn Arg <210> 173 <211> 1371 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1371) <223> Streptococcus pneumoniae spr1511 gene <400> 173 atggctagag aaggcttttt tacaggtcta gatattggaa caagctctgt caaggtgctt 60 gtggccgagc agagaaatgg tgaattaaat gtaattggcg tgagtaatgc caaaagtaaa 120 ggtgtaaagg atggaattat tgttgatatt gatgcagcag caactgctat caagtcagcc 180 atttcccaag cggaagaaaa ggcaggcatt tcgattaaat cagtgaatgt cggcttgcct 240 ggtaatcttt tgcaggtaga accaactcag gggatgattc cagtaacatc tgatactaag 300 gaaattacgg atcaagatgt tgaaaatgtt gtcaaatcag ctttgacaaa gagtatgaca 360 cctgaccgtg aagtcattac ctttattcct gaagaattta ttgtggatgg tttccaaggg 420 attcgtgacc cacgtggcat gatgggggtt cgccttgaaa tgcgtggttt gctttataca 480 ggacctcgta ctatcttgca caatttgcgt aagacggttg agcgtgcagg tgttcaggtt 540 gaaaatgtta tcatttcacc actagcaatg gttcagtctg ttttgaacga aggggaacgt 600 gaatttggtg ctacagtgat tgatatgggg gcaggtcaaa cgactgtcgc tacaatccgt 660 aatcaagaac tccagttcac acatattctc caagaaggtg gagattatgt aactaaagat 720 atctccaagg ttttgaaaac ctctcgcaaa ttagcggaag gcttaaaact gaattacggg 780 gaagcctatc cgcctcttgc aagcaaagaa accttccaag tagaggttat tggagaagta 840 gaagcagtcg aagtgacgga agcctacttg tcagaaatta tttctgcacg aatcaagcac 900 atccttgaac aaatcaagca agaattagat agaaggcgtc tattggacct ccctggtggt 960 attgtcttaa tcggtgggaa tgccatttta ccaggtatgg ttgagcttgc tcaggaagtc 1020 tttggcgtcc gtgtcaagct ttatgttcca aatcaagttg gtatccgtaa tccagccttt 1080 gcgcatgtga ttagtttatc agaatttgcg ggtcaattaa cagaagttaa tcttttggct 1140 cagggagcga taaaaggtga gaatgactta agtcatcagc caattagttt tggtgggatg 1200 ctgcaaaaaa cagctcagtt tgtacaatca acgcctgttc aaccagctcc tgctccagaa 1260 gtagagccgg tggcgcctac agaaccaatg gcggatttcc aacaagcttc acaaaataaa 1320 ccgaaattag cagatcgttt ccgtggcttg atcggaagca tgtttgacga a 1371 <210> 174 <211> 457 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (457) <223> Cell division protein FtsA <400> 174 Met Ala Arg Glu Gly Phe Phe Thr Gly Leu Asp Ile Gly Thr Ser Ser 1 5 10 15 Val Lys Val Leu Val Ala Glu Gln Arg Asn Gly Glu Leu Asn Val Ile 20 25 30 Gly Val Ser Asn Ala Lys Ser Lys Gly Val Lys Asp Gly Ile Ile Val 35 40 45 Asp Ile Asp Ala Ala Ala Thr Ala Ile Lys Ser Ala Ile Ser Gln Ala 50 55 60 Glu Glu Lys Ala Gly Ile Ser Ile Lys Ser Val Asn Val Gly Leu Pro 65 70 75 80 Gly Asn Leu Leu Gln Val Glu Pro Thr Gln Gly Met Ile Pro Val Thr 85 90 95 Ser Asp Thr Lys Glu Ile Thr Asp Gln Asp Val Glu Asn Val Val Lys 100 105 110 Ser Ala Leu Thr Lys Ser Met Thr Pro Asp Arg Glu Val Ile Thr Phe 115 120 125 Ile Pro Glu Glu Phe Ile Val Asp Gly Phe Gln Gly Ile Arg Asp Pro 130 135 140 Arg Gly Met Met Gly Val Arg Leu Glu Met Arg Gly Leu Leu Tyr Thr 145 150 155 160 Gly Pro Arg Thr Ile Leu His Asn Leu Arg Lys Thr Val Glu Arg Ala 165 170 175 Gly Val Gln Val Glu Asn Val Ile Ile Ser Pro Leu Ala Met Val Gln 180 185 190 Ser Val Leu Asn Glu Gly Glu Arg Glu Phe Gly Ala Thr Val Ile Asp 195 200 205 Met Gly Ala Gly Gln Thr Thr Val Ala Thr Ile Arg Asn Gln Glu Leu 210 215 220 Gln Phe Thr His Ile Leu Gln Glu Gly Gly Asp Tyr Val Thr Lys Asp 225 230 235 240 Ile Ser Lys Val Leu Lys Thr Ser Arg Lys Leu Ala Glu Gly Leu Lys 245 250 255 Leu Asn Tyr Gly Glu Ala Tyr Pro Pro Leu Ala Ser Lys Glu Thr Phe 260 265 270 Gln Val Glu Val Ile Gly Glu Val Glu Ala Val Glu Val Thr Glu Ala 275 280 285 Tyr Leu Ser Glu Ile Ile Ser Ala Arg Ile Lys His Ile Leu Glu Gln 290 295 300 Ile Lys Gln Glu Leu Asp Arg Arg Arg Leg Leu Asp Leu Pro Gly Gly 305 310 315 320 Ile Val Leu Ile Gly Gly Asn Ala Ile Leu Pro Gly Met Val Glu Leu 325 330 335 Ala Gln Glu Val Phe Gly Val Arg Val Lys Leu Tyr Val Pro Asn Gln 340 345 350 Val Gly Ile Arg Asn Pro Ala Phe Ala His Val Ile Ser Leu Ser Glu 355 360 365 Phe Ala Gly Gln Leu Thr Glu Val Asn Leu Leu Ala Gln Gly Ala Ile 370 375 380 Lys Gly Glu Asn Asp Leu Ser His Gln Pro Ile Ser Phe Gly Gly Met 385 390 395 400 Leu Gln Lys Thr Ala Gln Phe Val Gln Ser Thr Pro Val Gln Pro Ala 405 410 415 Pro Ala Pro Glu Val Glu Pro Val Ala Pro Thr Glu Pro Met Ala Asp 420 425 430 Phe Gln Gln Ala Ser Gln Asn Lys Pro Lys Leu Ala Asp Arg Phe Arg 435 440 445 Gly Leu Ile Gly Ser Met Phe Asp Glu 450 455 <175> 175 <211> 1371 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1371) <223> Streptococcus pneumoniae spr1514 gene <400> 175 atgaaattaa caatccatga aattgcccaa gttgttggag ccaaaaatga tatcagtatc 60 tttgaggaca ctcagttaga aaaagctgag tttgatagtc gtttgattgg aactggagat 120 ttatttgtgc cacttaaagg tgcgcgtgat ggccatgact ttattgaaac agcctttgaa 180 aatggtgcag cagtaacctt gtctgagaaa gaggtctcaa atcatcctta cattttagta 240 gatgatgttt tgacagcctt tcaatcccta gcatcctact atcttgaaaa aacgactgtt 300 gatgtctttg ctgttacagg ttcaaatggc aagacaacga ctaaggatat gttggcgcat 360 ttactgtcaa caagatacaa gacctacaaa acacaaggca attacaataa tgagattggc 420 cttccttaca cagttcttca tatgcctgaa ggaacagaaa agttggtttt ggagatggga 480 caggatcact tgggagatat tcatctcttg tctgaattgg ctcgtccaaa aacagccatc 540 gtgaccttgg ttggagaagc tcatttggcc tttttcaaag atcgttctga gattgctaaa 600 ggaaaaatgc aaattgcaga cggaatggct tcaggttcct tgcttttagc gccggctgac 660 cctatcgtag aggactattt gccaattgat aaaaaggtgg ttcgttttgg tcaaggagca 720 gagctggaaa ttactgactt ggttgagcgt aaggacagtc tgacctttaa ggctaatttc 780 ttggaacaag cccttgattt gccagtaact ggcaagtaca atgcgacaaa tgctatgatt 840 gcatcctatg ttgccttgca agaaggagtt tcagaggagc aaattcgttt ggcattccaa 900 catcttgaat tgacgcgtaa ccgtaccgag tggaagaaag cagccaatgg agcagatatc 960 ctgtcagatg tttacaatgc caatccaact gctatgaaac tgattttaga gactttctct 1020 gccattccag ccaatgaagg tggcaagaaa attgcagtgt tggcggatat gaaggagctt 1080 ggtgaccagt ctgttcaact tcataatcag atgattttga gcctctcccc agatgtgctt 1140 gatatcgtta ttttctatgg agaagatatt gctcagttgg ctcaattggc cagtcaaatg 1200 ttcccaatcg gccacgttta ctacttcaag aaaacagaag accaggatca atttgaagac 1260 ctagtcaagc aggtcaagga aagccttgga gcccatgacc aaatcctgct caaaggctct 1320 aactctatga atctagccaa gttggtagaa agtttagaaa atgaagacaa g 1371 <210> 176 <211> 457 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (457) UDP-N-acetylmuramoylalanine-D-glutamyl-lysine-D-alanyl-D-alanine ligase <400> 176 Met Lys Leu Thr Ile His Glu Ile Ala Gln Val Val Gly Ala Lys Asn 1 5 10 15 Asp Ile Ser Ile Phe Glu Asp Thr Gln Leu Glu Lys Ala Glu Phe Asp 20 25 30 Ser Arg Leu Ile Gly Thr Gly Asp Leu Phe Val Pro Leu Lys Gly Ala 35 40 45 Arg Asp Gly His Asp Phe Ile Glu Thr Ala Phe Glu Asn Gly Ala Ala 50 55 60 Val Thr Leu Ser Glu Lys Glu Val Ser Asn His Pro Tyr Ile Leu Val 65 70 75 80 Asp Asp Val Leu Thr Ala Phe Gln Ser Leu Ala Ser Tyr Tyr Leu Glu 85 90 95 Lys Thr Thr Val Asp Val Phe Ala Val Thr Gly Ser Asn Gly Lys Thr 100 105 110 Thr Thr Lys Asp Met Leu Ala His Leu Leu Ser Thr Arg Tyr Lys Thr 115 120 125 Tyr Lys Thr Gln Gly Asn Tyr Asn Asn Glu Ile Gly Leu Pro Tyr Thr 130 135 140 Val Leu His Met Pro Glu Gly Thr Glu Lys Leu Val Leu Glu Met Gly 145 150 155 160 Gln Asp His Leu Gly Asp Ile His Leu Leu Ser Glu Leu Ala Arg Pro 165 170 175 Lys Thr Ala Ile Val Thr Leu Val Gly Glu Ala His Leu Ala Phe Phe 180 185 190 Lys Asp Arg Ser Glu Ile Ala Lys Gly Lys Met Gln Ile Ala Asp Gly 195 200 205 Met Ala Ser Gly Ser Leu Leu Leu Ala Pro Ala Asp Pro Ile Val Glu 210 215 220 Asp Tyr Leu Pro Ile Asp Lys Lys Val Val Arg Phe Gly Gln Gly Ala 225 230 235 240 Glu Leu Glu Ile Thr Asp Leu Val Glu Arg Lys Asp Ser Leu Thr Phe 245 250 255 Lys Ala Asn Phe Leu Glu Gln Ala Leu Asp Leu Pro Val Thr Gly Lys 260 265 270 Tyr Asn Ala Thr Asn Ala Met Ile Ala Ser Tyr Val Ala Leu Gln Glu 275 280 285 Gly Val Ser Glu Glu Gln Ile Arg Leu Ala Phe Gln His Leu Glu Leu 290 295 300 Thr Arg Asn Arg Thr Glu Trp Lys Lys Ala Ala Asn Gly Ala Asp Ile 305 310 315 320 Leu Ser Asp Val Tyr Asn Ala Asn Pro Thr Ala Met Lys Leu Ile Leu 325 330 335 Glu Thr Phe Ser Ala Ile Pro Ala Asn Glu Gly Gly Lys Lys Ile Ala 340 345 350 Val Leu Ala Asp Met Lys Glu Leu Gly Asp Gln Ser Val Gln Leu His 355 360 365 Asn Gln Met Ile Leu Ser Leu Ser Pro Asp Val Leu Asp Ile Val Ile 370 375 380 Phe Tyr Gly Glu Asp Ile Ala Gln Leu Ala Gln Leu Ala Ser Gln Met 385 390 395 400 Phe Pro Ile Gly His Val Tyr Tyr Phe Lys Lys Thr Glu Asp Gln Asp 405 410 415 Gln Phe Glu Asp Leu Val Lys Gln Val Lys Glu Ser Leu Gly Ala His 420 425 430 Asp Gln Ile Leu Leu Lys Gly Ser Asn Ser Met Asn Leu Ala Lys Leu 435 440 445 Val Glu Ser Leu Glu Asn Glu Asp Lys 450 455 <210> 177 <211> 1041 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1041) <223> Streptococcus pneumoniae spr1515 gene <400> 177 atgaaacaaa cgattattct tttatatggt ggacggagtg cggaacgcga agtctctgtc 60 ctttcagctg agagtgtcat gcgtgcggtc gattacgacc gtttcacagt caagactttc 120 tttatcagtc agtcaggtga ctttatcaaa acacaggaat ttagtcatgc tccggggcaa 180 gaagaccgtc tcatgaccaa tgaaaccatt gattgggata agaaagttgc accaagtgct 240 atctacgaag aaggtgcagt ggtctttcca gtccttcacg ggccaatggg agaagatggc 300 tctgttcaag gattcttgga agttttgaaa atgccttacg ttggttgcaa cattttgtca 360 tcaagtcttg ccatggataa aatcacgact aagcgtgttc tggaatctgc tggtattgcc 420 caagttcctt atgtggctat cgttgaaggc gatgatgtga ctgctaaaat cgctgaagtg 480 gaagaaaaat tggcttatcc agtcttcact aagccgtcaa acatggggtc tagtgtcggt 540 atttctaagt ctgaaaacca agaagaactc cgtcaagcct taaaacttgc cttccgatat 600 gacagccgtg tcttggttga gcaaggagtg aatgcccgtg aaattgaggt tggcctcttg 660 ggtaactacg atgtcaagag cacgctacct ggagaagttg tcaaggacgt tgccttttat 720 gactacgatg ccaagtatat tgataacaag gttactatgg atattcctgc caaaatcagt 780 gatgatgtgg tggctgtcat gcgtcaaaat gcagaaacag ccttccgtgc cattggtggc 840 cttggtctat ctcgttgcga tttcttctat acagataagg gagagatttt tctcaacgag 900 ctcaatacca tgccaggttt cacccagtgg tctatgtacc cactactttg ggaaaatatg 960 gggatcagct acccagaact aatcgagcgt ttggttgacc ttgccaagga aagttttgac 1020 aagcgcgaag cgcatttgat a 1041 <210> 178 <211> 347 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (347) <223> D-alanine-D-alanine ligase <400> 178 Met Lys Gln Thr Ile Ile Leu Leu Tyr Gly Gly Arg Ser Ala Glu Arg 1 5 10 15 Glu Val Ser Val Leu Ser Ala Glu Ser Val Met Arg Ala Val Asp Tyr 20 25 30 Asp Arg Phe Thr Val Lys Thr Phe Ile Ser Gln Ser Gly Asp Phe 35 40 45 Ile Lys Thr Gln Glu Phe Ser His Ala Pro Gly Gln Glu Asp Arg Leu 50 55 60 Met Thr Asn Glu Thr Ile Asp Trp Asp Lys Lys Val Ala Pro Ser Ala 65 70 75 80 Ile Tyr Glu Glu Gly Ala Val Val Phe Pro Val Leu His Gly Pro Met 85 90 95 Gly Glu Asp Gly Ser Val Gln Gly Phe Leu Glu Val Leu Lys Met Pro 100 105 110 Tyr Val Gly Cys Asn Ile Leu Ser Ser Ser Leu Ala Met Asp Lys Ile 115 120 125 Thr Thr Lys Arg Val Leu Glu Ser Ala Gly Ile Ala Gln Val Pro Tyr 130 135 140 Val Ala Ile Val Glu Gly Asp Asp Val Thr Ala Lys Ile Ala Glu Val 145 150 155 160 Glu Glu Lys Leu Ala Tyr Pro Val Phe Thr Lys Pro Ser Asn Met Gly 165 170 175 Ser Ser Val Gly Ile Ser Lys Ser Glu Asn Gln Glu Glu Leu Arg Gln 180 185 190 Ala Leu Lys Leu Ala Phe Arg Tyr Asp Ser Arg Val Leu Val Glu Gln 195 200 205 Gly Val Asn Ala Arg Glu Ile Glu Val Gly Leu Leu Gly Asn Tyr Asp 210 215 220 Val Lys Ser Thr Leu Pro Gly Glu Val Val Lys Asp Val Ala Phe Tyr 225 230 235 240 Asp Tyr Asp Ala Lys Tyr Ile Asp Asn Lys Val Thr Met Asp Ile Pro 245 250 255 Ala Lys Ile Ser Asp Asp Val Val Ala Val Met Arg Gln Asn Ala Glu 260 265 270 Thr Ala Phe Arg Ala Ile Gly Gly Leu Gly Leu Ser Arg Cys Asp Phe 275 280 285 Phe Tyr Thr Asp Lys Gly Glu Ile Phe Leu Asn Glu Leu Asn Thr Met 290 295 300 Pro Gly Phe Thr Gln Trp Ser Met Tyr Pro Leu Leu Trp Glu Asn Met 305 310 315 320 Gly Ile Ser Tyr Pro Glu Leu Ile Glu Arg Leu Val Asp Leu Ala Lys 325 330 335 Glu Ser Phe Asp Lys Arg Glu Ala His Leu Ile 340 345 <210> 179 <211> 450 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (450) <223> Streptococcus pneumoniae spr1535 gene <400> 179 atgattatta caaaaattag ccgtttagga acttatgtgg gagtaaatcc acattttgca 60 acattaatag attttctaga aaaaacagga ctagaaaatt taacagaagg ttcgattgct 120 atcgatggta atcgattgtt tgggaattgc tttacttatc tagcagatgg tcaagcaggg 180 gctttctttg aaacacacca aaaatatttg gatattcatt tagttttgga aaacgaagaa 240 gccatggctg ttacatcgcc ggaaaatgta agcgttaccc aagaatatga tgaagagaaa 300 gatattgaat tatacacagg gaaagtggaa cagttggttc atttgagagc tggtgaatgc 360 ctcatcactt ttccagaaga tttacatcaa cccaaggttc gtataaatga tgaacctgtg 420 aaaaaagttg tctttaaagt tgcgatttct 450 <210> 180 <211> 150 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (150) <223> Conserved hypothetical protein <400> 180 Met Ile Ile Thr Lys Ile Ser Arg Leu Gly Thr Tyr Val Gly Val Asn 1 5 10 15 Pro His Phe Ala Thr Leu Ile Asp Phe Leu Glu Lys Thr Gly Leu Glu 20 25 30 Asn Leu Thr Glu Gly Ser Ile Ala Ile Asp Gly Asn Arg Leu Phe Gly 35 40 45 Asn Cys Phe Thr Tyr Leu Ala Asp Gly Gln Ala Gly Ala Phe Phe Glu 50 55 60 Thr His Gln Lys Tyr Leu Asp Ile His Leu Val Leu Glu Asn Glu Glu 65 70 75 80 Ala Met Ala Val Thr Ser Pro Glu Asn Val Ser Val Thr Gln Glu Tyr 85 90 95 Asp Glu Glu Lys Asp Ile Glu Leu Tyr Thr Gly Lys Val Glu Gln Leu 100 105 110 Val His Leu Arg Ala Gly Glu Cys Leu Ile Thr Phe Pro Glu Asp Leu 115 120 125 His Gln Pro Lys Val Arg Ile Asn Asp Glu Pro Val Lys Lys Val Val 130 135 140 Phe Lys Val Ala Ile Ser 145 150 <210> 181 <211> 1122 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1122) <223> Streptococcus pneumoniae spr1540 gene <400> 181 gtgtcatttt ggaggaaaat catgaaagct agtccacata gaccaaccaa ggctctgatt 60 catctgggag ctattcgaca aaatattcag caaatggggg ctcatatccc tcaaggaacg 120 ctcaagttgg ctgtggttaa ggccaatgct tatggtcatg gagctgttgc cgttgccaag 180 gcaattcaag atgatgttga tggcttttgc gtttccaata tcgatgaagc cattgaactc 240 agacaagctg gactcagcaa gccaatcctc attttaggag tttctgaaat cgaagctgtt 300 gctctagcta aagaatatga cttcaccttg acagtggctg gactggagtg gattcaagca 360 ctcttagata aggaagtgga cctaactgga ttgacagtcc acctcaagat tgattcaggg 420 atgggacgga ttggttttag agaggcaagt gaggttgagc aggctcaaga tttgctccaa 480 caacacggtg tttgtgttga aggaatcttt acccactttg ctactgctga tgaggaatca 540 gatgactatt ttaatgccca gttagaacgg tttaaaacta ttttagctag tatgaaggaa 600 gttccagagc tggttcatgc tagcaattct gcaacgactc tttggcatgt agagactatt 660 ttcaatgcgg ttcgtatggg agatgccatg tatggtctca atccaagtgg agcggtcttg 720 gatttgcctt atgatttgat accggccttg accttggagt ctgctctggt tcatgtcaag 780 acagttccag ctggagcttg catgggctat ggagcaactt atcaagcgga tagcgagcaa 840 gtcatcgcga ccgtgccaat cgggtatgca gatggatgga caagagacat gcaaaatttc 900 tctgtcttgg tagatggcca agcttgccca attgtcggca gggtttcgat ggaccaaatc 960 actattcgat tgcctaagct ttatccgcta ggaaccaagg taaccttgat tggctccaat 1020 ggggataagg aaatcactgc aactcaggta gcgacctacc gcgtaaccat taactatgag 1080 gtggtttgcc tcctcagcga ccgtattccg agagaatatt at 1122 <210> 182 <211> 374 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (374) Alanine racemase <400> 182 Met Ser Phe Trp Arg Lys Ile Met Lys Ala Ser Pro His Arg Pro Thr 1 5 10 15 Lys Ala Leu Ile His Leu Gly Ala Ile Arg Gln Asn Ile Gln Gln Met 20 25 30 Gly Ala His Ile Pro Gln Gly Thr Leu Lys Leu Ala Val Val Lys Ala 35 40 45 Asn Ala Tyr Gly His Gly Ala Val Ala Val Ala Lys Ala Ile Gln Asp 50 55 60 Asp Val Asp Gly Phe Cys Val Ser Asn Ile Asp Glu Ala Ile Glu Leu 65 70 75 80 Arg Gln Ala Gly Leu Ser Lys Pro Ile Leu Ile Leu Gly Val Ser Glu 85 90 95 Ile Glu Ala Val Ala Leu Ala Lys Glu Tyr Asp Phe Thr Leu Thr Val 100 105 110 Ala Gly Leu Glu Trp Ile Gln Ala Leu Leu Asp Lys Glu Val Asp Leu 115 120 125 Thr Gly Leu Thr Val His Leu Lys Ile Asp Ser Gly Met Gly Arg Ile 130 135 140 Gly Phe Arg Glu Ala Ser Glu Val Glu Gln Ala Gln Asp Leu Leu Gln 145 150 155 160 Gln His Gly Val Cys Val Glu Gly Ile Phe Thr His Phe Ala Thr Ala 165 170 175 Asp Glu Glu Ser Asp Asp Tyr Phe Asn Ala Gln Leu Glu Arg Phe Lys 180 185 190 Thr Ile Leu Ala Ser Met Lys Glu Val Pro Glu Leu Val His Ala Ser 195 200 205 Asn Ser Ala Thr Thr Leu Trp His Val Glu Thr Ile Phe Asn Ala Val 210 215 220 Arg Met Gly Asp Ala Met Tyr Gly Leu Asn Pro Ser Gly Ala Val Leu 225 230 235 240 Asp Leu Pro Tyr Asp Leu Ile Pro Ala Leu Thr Leu Glu Ser Ala Leu 245 250 255 Val His Val Lys Thr Val Pro Ala Gly Ala Cys Met Gly Tyr Gly Ala 260 265 270 Thr Tyr Gln Ala Asp Ser Glu Gln Val Ile Ala Thr Val Pro Ile Gly 275 280 285 Tyr Ala Asp Gly Trp Thr Arg Asp Met Gln Asn Phe Ser Val Leu Val 290 295 300 Asp Gly Gln Ala Cys Pro Ile Val Gly Arg Val Ser Met Asp Gln Ile 305 310 315 320 Thr Ile Arg Leu Pro Lys Leu Tyr Pro Leu Gly Thr Lys Val Thr Leu 325 330 335 Ile Gly Ser Asn Gly Asp Lys Glu Ile Thr Ala Thr Gln Val Ala Thr 340 345 350 Tyr Arg Val Thr Ile Asn Tyr Glu Val Val Cys Leu Leu Ser Asp Arg 355 360 365 Ile Pro Arg Glu Tyr Tyr 370 <210> 183 <211> 360 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (360) <223> Streptococcus pneumoniae spr1541 gene <400> 183 atgatagttg gacacggaat tgacatcgaa gaattggctt cgatagaaag cgcagttaca 60 cgacatgaag gatttgctaa gcgtgtactg accgctcagg aaatggagcg cttcaccagt 120 ctcaaaggac gcaggcaaat agaatattta gctggtcgct ggtcggctaa ggaggccttt 180 tccaaggcta tgggaacggg cattagcaag ctcggttttc aggatttgga agtcttgaac 240 aatgaacgtg gggcgcctta ttttagtcag gcaccatttt caggaaagat ttggctgtct 300 atcagccaca ccgatcagtt tgtgacagcc agtgtcattt tggaggaaaa tcatgaaagc 360 360 <210> 184 <211> 120 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN <222> (1) .. (120) <223> Acyl Carrier protein synthase <400> 184 Met Ile Val Gly His Gly Ile Asp Ile Glu Glu Leu Ala Ser Ile Glu 1 5 10 15 Ser Ala Val Thr Arg His Glu Gly Phe Ala Lys Arg Val Leu Thr Ala 20 25 30 Gln Glu Met Glu Arg Phe Thr Ser Leu Lys Gly Arg Arg Gln Ile Glu 35 40 45 Tyr Leu Ala Gly Arg Trp Ser Ala Lys Glu Ala Phe Ser Lys Ala Met 50 55 60 Gly Thr Gly Ile Ser Lys Leu Gly Phe Gln Asp Leu Glu Val Leu Asn 65 70 75 80 Asn Glu Arg Gly Ala Pro Tyr Phe Ser Gln Ala Pro Phe Ser Gly Lys 85 90 95 Ile Trp Leu Ser Ile Ser His Thr Asp Gln Phe Val Thr Ala Ser Val 100 105 110 Ile Leu Glu Glu Asn His Glu Ser 115 120 <210> 185 <211> 1308 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (1) .. (1308) <223> Streptococcus pneumoniae spr1553 gene <400> 185 atggccctac caactattgc cattgtagga cgtcccaatg ttgggaaatc aaccctattt 60 aatcggatcg ctggtgagcg aatctccatt gtagaagatg tcgaaggagt gacacgtgac 120 cgtatttatg caacgggtga gtggctcaat cgttctttta gcatgattga tacaggagga 180 attgatgatg tcgatgctcc tttcatggaa caaatcaagc accaggcaga aattgccatg 240 gaagaagcag atgttatcgt ctttgtcgtg tccggtaagg aaggaattac cgatgcagac 300 gaatacgtag cccgtaagct ttataagacc cacaaaccag ttatcctcgc agttaacaag 360 gtggacaacc ctgagatgcg aaatgatatc tatgatttct atgccctcgg tttgggtgaa 420 ccactgccta tctcatctgt ccatggtatc ggtacagggg atgtactaga tgcgattgtg 480 gaaaaccttc caaatgaata tgaagaagaa aatccagatg tcattaagtt tagcttgatt 540 ggtcgtccta acgttggaaa atcaagcttg attaatgcta tcttgggaga agaccgtgtc 600 attgccagtc ctgttgctgg aacaactcgt gacgctattg atacccactt tacagataca 660 gatggtcaag agtttaccat gattgatacg gctggtatgc gtaagtctgg taaggtttat 720 gaaaatactg agaagtactc tgtcatgcgt gccatgcgtg ctattgaccg ttcagatgtg 780 gtcttaatgg tcatcaatgc ggaagagggg attcgtgaat acgacaagcg tatcgctgga 840 tttgctcatg aagctggtaa agggatgatt atcgtggtca acaagtggga tacgcttgaa 900 aaagataacc acactatgaa aaactgggaa gaagatatcc gtgagcagtt ccaatacctg 960 ccttacgcac cgattatctt tgtatcagct ttaaccaagc aacgtctcca caaacttcct 1020 gagatgatta agcaaatcag cgaaagtcaa aatacacgta ttccatcagc tgtcttgaac 1080 gatgtcatca tggatgccat tgccatcaac ccaacaccga cagacaaagg aaaacgtctc 1140 aagattttct atgcgaccca agtggcaacc aaaccaccaa cctttgtcat ctttgtcaat 1200 gaagaagaac tcatgcactt ttcttacctg cgtttcttgg aaaatcaaat ccgcaaggcc 1260 tttgtttttg agggaacacc gattcatctc atcgcaagaa aacgcaaa 1308 <210> 186 <211> 436 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (436) <223> Phosphoglycerate dehydrogenase <400> 186 Met Ala Leu Pro Thr Ile Ala Ile Val Gly Arg Pro Asn Val Gly Lys 1 5 10 15 Ser Thr Leu Phe Asn Arg Ile Ala Gly Glu Arg Ile Ser Ile Val Glu 20 25 30 Asp Val Glu Gly Val Thr Arg Asp Arg Ile Tyr Ala Thr Gly Glu Trp 35 40 45 Leu Asn Arg Ser Phe Ser Met Ile Asp Thr Gly Gly Ile Asp Asp Val 50 55 60 Asp Ala Pro Phe Met Glu Gln Ile Lys His Gln Ala Glu Ile Ala Met 65 70 75 80 Glu Glu Ala Asp Val Ile Val Phe Val Val Ser Gly Lys Glu Gly Ile 85 90 95 Thr Asp Ala Asp Glu Tyr Val Ala Arg Lys Leu Tyr Lys Thr His Lys 100 105 110 Pro Val Ile Leu Ala Val Asn Lys Val Asp Asn Pro Glu Met Arg Asn 115 120 125 Asp Ile Tyr Asp Phe Tyr Ala Leu Gly Leu Gly Glu Pro Leu Pro Ile 130 135 140 Ser Ser Val His Gly Ile Gly Thr Gly Asp Val Leu Asp Ala Ile Val 145 150 155 160 Glu Asn Leu Pro Asn Glu Tyr Glu Glu Glu Asn Pro Asp Val Ile Lys 165 170 175 Phe Ser Leu Ile Gly Arg Pro Asn Val Gly Lys Ser Ser Leu Ile Asn 180 185 190 Ala Ile Leu Gly Glu Asp Arg Val Ile Ala Ser Pro Val Ala Gly Thr 195 200 205 Thr Arg Asp Ala Ile Asp Thr His Phe Thr Asp Thr Asp Gly Gln Glu 210 215 220 Phe Thr Met Ile Asp Thr Ala Gly Met Arg Lys Ser Gly Lys Val Tyr 225 230 235 240 Glu Asn Thr Glu Lys Tyr Ser Val Met Arg Ala Met Arg Ala Ile Asp 245 250 255 Arg Ser Asp Val Val Leu Met Val Ile Asn Ala Glu Glu Gly Ile Arg 260 265 270 Glu Tyr Asp Lys Arg Ile Ala Gly Phe Ala His Glu Ala Gly Lys Gly 275 280 285 Met Ile Ile Val Val Asn Lys Trp Asp Thr Leu Glu Lys Asp Asn His 290 295 300 Thr Met Lys Asn Trp Glu Glu Asp Ile Arg Glu Gln Phe Gln Tyr Leu 305 310 315 320 Pro Tyr Ala Pro Ile Ile Phe Val Ser Ala Leu Thr Lys Gln Arg Leu 325 330 335 His Lys Leu Pro Glu Met Ile Lys Gln Ile Ser Glu Ser Gln Asn Thr 340 345 350 Arg Ile Pro Ser Ala Val Leu Asn Asp Val Ile Met Asp Ala Ile Ala 355 360 365 Ile Asn Pro Thr Pro Thr Asp Lys Gly Lys Arg Leu Lys Ile Phe Tyr 370 375 380 Ala Thr Gln Val Ala Thr Lys Pro Pro Thr Phe Val Ile Phe Val Asn 385 390 395 400 Glu Glu Glu Leu Met His Phe Ser Tyr Leu Arg Phe Leu Glu Asn Gln 405 410 415 Ile Arg Lys Ala Phe Val Phe Glu Gly Thr Pro Ile His Leu Ile Ala 420 425 430 Arg Lys Arg Lys 435 <210> 187 <211> 894 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (894) <223> Streptococcus pneumoniae spr1555 gene <400> 187 atggaaagtg tcggagatgt actcaaacgt caaccaagtc gttttcacta tcaagattta 60 gttcagaaaa tcatgaagga tcctgatgtt gcggcctttg tccagcaaga atccctcaat 120 caggacgagt tgaaccgtag tatttccaag tttaaccaat acatcactga gagagataaa 180 ttcctccgag gagatacaga ttatattgcc aaaggctaca agcctatatt agtcatgaac 240 catggctatg cggatgtttc ttatgaagaa actcctgaac taatcgcagc tgaaaaagaa 300 gcggctatca agaaacgtct caacttaatt aattttccat ctagcctgaa aaatgtcagt 360 tttttagatg tttatcgtga tgatgttcag cgcttaactg ttctaaaaag aatgatagaa 420 tttgttaacg attaccccaa taatttgaaa ggtctttact tatatggaga ctttggtgtg 480 ggtaaaagtt tcatggtggc ggccttagct catgatttat cagaaaaacg tggtgtttca 540 tcaactctcc tccactatcc tagctttgtc attgatgtca aaaatgctat cagtgatggc 600 aatgttaaga ccttggtgga tgagattaag ctgtctgagg tcctgatttt agatgatatt 660 ggcgccgagc aatcaaccgt ttgggtgcgt gacgaaatcc tgcaggtcat tctccaatat 720 cggatgcagg aaaatttacc gacctttttc acatccaact tcaactttga agatttggag 780 aagcattttg cgaaagtgaa acatggaaat gacgaaacct gggaagccag acgggtcatg 840 gaacgcatcc gttatttggc tgaggagact cgtttagaag gagtaaaccg tcga 894 <210> 188 <211> 298 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (298) <223> Primosome component <400> 188 Met Glu Ser Val Gly Asp Val Leu Lys Arg Gln Pro Ser Arg Phe His 1 5 10 15 Tyr Gln Asp Leu Val Gln Lys Ile Met Lys Asp Pro Asp Val Ala Ala 20 25 30 Phe Val Gln Gln Glu Ser Leu Asn Gln Asp Glu Leu Asn Arg Ser Ile 35 40 45 Ser Lys Phe Asn Gln Tyr Ile Thr Glu Arg Asp Lys Phe Leu Arg Gly 50 55 60 Asp Thr Asp Tyr Ile Ala Lys Gly Tyr Lys Pro Ile Leu Val Met Asn 65 70 75 80 His Gly Tyr Ala Asp Val Ser Tyr Glu Glu Thr Pro Glu Leu Ile Ala 85 90 95 Ala Glu Lys Glu Ala Ala Ile Lys Lys Arg Leu Asn Leu Ile Asn Phe 100 105 110 Pro Ser Ser Leu Lys Asn Val Ser Phe Leu Asp Val Tyr Arg Asp Asp 115 120 125 Val Gln Arg Leu Thr Val Leu Lys Arg Met Ile Glu Phe Val Asn Asp 130 135 140 Tyr Pro Asn Asn Leu Lys Gly Leu Tyr Leu Tyr Gly Asp Phe Gly Val 145 150 155 160 Gly Lys Ser Phe Met Val Ala Ala Leu Ala His Asp Leu Ser Glu Lys 165 170 175 Arg Gly Val Ser Ser Thr Leu Leu His Tyr Pro Ser Phe Val Ile Asp 180 185 190 Val Lys Asn Ala Ile Ser Asp Gly Asn Val Lys Thr Leu Val Asp Glu 195 200 205 Ile Lys Leu Ser Glu Val Leu Ile Leu Asp Asp Ile Gly Ala Glu Gln 210 215 220 Ser Thr Val Trp Val Arg Asp Glu Ile Leu Gln Val Ile Leu Gln Tyr 225 230 235 240 Arg Met Gln Glu Asn Leu Pro Thr Phe Phe Thr Ser Asn Phe Asn Phe 245 250 255 Glu Asp Leu Glu Lys His Phe Ala Lys Val Lys His Gly Asn Asp Glu 260 265 270 Thr Trp Glu Ala Arg Arg Val Met Glu Arg Ile Arg Tyr Leu Ala Glu 275 280 285 Glu Thr Arg Leu Glu Gly Val Asn Arg Arg 290 295 <210> 189 <211> 1194 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1194) <223> Streptococcus pneumoniae spr1571 gene <400> 189 atgaatgata aaacagaggt aaatatgaca atcggtattg ataagattgg ttttgcgacc 60 agtcaatatg tcttgaaatt acaagactta gcagaagcga ggggaattga ccctgaaaaa 120 ttaagtaaag gactcttact caaggaattg agtattgcgc ccctaactga ggacatcgtg 180 accttggcgg ccagtgctag tgactctatt ttaactgagc aagaaagaca agaagttgac 240 atggtcattg tggctaccga gtcaggaatt gaccagagta aggctgcggc cgtctttgtg 300 catggcttgc tgggcatcca gccctttgct cgtagtttcg agattaaaga agcctgctat 360 ggggcgacag ctgccctcca ttatgccaaa ttgcatgtgg aaaattctcc ggagtccaag 420 gtcttggtca ttgccagtga tattgccaaa tatggtattg aaactccggg agaaccaact 480 caaggtgctg gaagtgtagc tatgttgatt acacaaaatc cacgcatgat ggcctttaat 540 aatgacaatg tagctcagac ccgtgacatc atggatttct ggcgaccaaa ttactcgaca 600 actccttatg taaatggtgt ctattctacc caacaatact tggatagttt gaaaacgact 660 tggcttgaat atcaaaaacg ctaccagctt actttggatg attttgcggc tgtttgtttc 720 cacttgcctt atcctaaatt agcgctaaaa ggcttgaaaa aaatcatgga taagagcctg 780 cctcaagaga aaaaagacct cttacaaaag cattttgacc agtctattct ctacagtcaa 840 aaggtgggga atatctacac aggttcactt ttccttggac ttttgtctct cttggaaaat 900 acagatagct tgaaagctgg ggataaaatc gtcctttata gttacggaag tggagctgtg 960 gctgagttct tcagtggtga attggttgaa ggatatgaag cttatttgga taaagaccgc 1020 ttgaacaagc tcaaccaacg aactgcccta tccgttgcag actatgaaaa ggtctttttt 1080 gaggaagtaa acttggatga aacaaactct gcccagtttg ctggctatga aaatcaagat 1140 tttgccttgg ttgaaattct tgaccaccaa cgccgttata gcaaggttga aaaa 1194 <210> 190 <211> 398 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (398) 3-223-3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase <400> 190 Met Asn Asp Lys Thr Glu Val Asn Met Thr Ile Gly Ile Asp Lys Ile 1 5 10 15 Gly Phe Ala Thr Ser Gln Tyr Val Leu Lys Leu Gln Asp Leu Ala Glu 20 25 30 Ala Arg Gly Ile Asp Pro Glu Lys Leu Ser Lys Gly Leu Leu Leu Lys 35 40 45 Glu Leu Ser Ile Ala Pro Leu Thr Glu Asp Ile Val Thr Leu Ala Ala 50 55 60 Ser Ala Ser Asp Ser Ile Leu Thr Glu Gln Glu Arg Gln Glu Val Asp 65 70 75 80 Met Val Ile Val Ala Thr Glu Ser Gly Ile Asp Gln Ser Lys Ala Ala 85 90 95 Ala Val Phe Val His Gly Leu Leu Gly Ile Gln Pro Phe Ala Arg Ser 100 105 110 Phe Glu Ile Lys Glu Ala Cys Tyr Gly Ala Thr Ala Ala Leu His Tyr 115 120 125 Ala Lys Leu His Val Glu Asn Ser Pro Glu Ser Lys Val Leu Val Ile 130 135 140 Ala Ser Asp Ile Ala Lys Tyr Gly Ile Glu Thr Pro Gly Glu Pro Thr 145 150 155 160 Gln Gly Ala Gly Ser Val Ala Met Leu Ile Thr Gln Asn Pro Arg Met 165 170 175 Met Ala Phe Asn Asn Asp Asn Val Ala Gln Thr Arg Asp Ile Met Asp 180 185 190 Phe Trp Arg Pro Asn Tyr Ser Thr Thr Pro Tyr Val Asn Gly Val Tyr 195 200 205 Ser Thr Gln Gln Tyr Leu Asp Ser Leu Lys Thr Thr Trp Leu Glu Tyr 210 215 220 Gln Lys Arg Tyr Gln Leu Thr Leu Asp Asp Phe Ala Ala Val Cys Phe 225 230 235 240 His Leu Pro Tyr Pro Lys Leu Ala Leu Lys Gly Leu Lys Lys Ile Met 245 250 255 Asp Lys Ser Leu Pro Gln Glu Lys Lys Asp Leu Leu Gln Lys His Phe 260 265 270 Asp Gln Ser Ile Leu Tyr Ser Gln Lys Val Gly Asn Ile Tyr Thr Gly 275 280 285 Ser Leu Phe Leu Gly Leu Leu Ser Leu Leu Glu Asn Thr Asp Ser Leu 290 295 300 Lys Ala Gly Asp Lys Ile Val Leu Tyr Ser Tyr Gly Ser Gly Ala Val 305 310 315 320 Ala Glu Phe Phe Ser Gly Glu Leu Val Glu Gly Tyr Glu Ala Tyr Leu 325 330 335 Asp Lys Asp Arg Leu Asn Lys Leu Asn Gln Arg Thr Ala Leu Ser Val 340 345 350 Ala Asp Tyr Glu Lys Val Phe Phe Glu Glu Val Asn Leu Asp Glu Thr 355 360 365 Asn Ser Ala Gln Phe Ala Gly Tyr Glu Asn Gln Asp Phe Ala Leu Val 370 375 380 Glu Ile Leu Asp His Gln Arg Arg Tyr Ser Lys Val Glu Lys 385 390 395 <210> 191 <211> 933 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (933) <223> Streptococcus pneumoniae spr1580 gene <400> 191 atgacaaaac taatctttat ggggaccccc gacttttcag caacagtctt aaaaggactt 60 ttgacagatg accgttacga aattctagcc gttgtgaccc agccagaccg tgctgttggt 120 cgtaaaaaag ttatccaaga aaccccagtc aagcaggctg ccaaggaagc aggattatct 180 atctaccaac ctgaaaaatt atctggaagt ccagagatgg aagatcttat gaagctagga 240 gcagatggaa ttgtgactgc tgcttttggg cagtttctcc caagcaaact ccttgatagc 300 atggactttg ctgtcaacgt tcatgcctcc ctccttccta gacaccgtgg tggtgcgcct 360 atccattatg ccttgattca aggggatgag gaagctggtg tgaccatcat ggaaatggtt 420 aaggaaatgg atgcaggaga tatgatttct cgtcgcagca ttccgatcac agatgaggac 480 aatgttggca ccttgtttga aaaattggcg ctagttggtc gtgatttgct tttggacact 540 ctgcctgcct atattgctgg tgatatcaaa cctgaaccgc aggatacgag tcaggttacc 600 ttctctccaa atataaagtc agaggaagaa aaactgaact ggaacaaaac caatcgtcaa 660 ctctttaacc aaattcgtgg aatgaacccc tggcctgttg cccatacttt ccttaagggc 720 gaccgcttta agatttatga agccctacca gtagaaggtc agggaaatcc aggtgagatt 780 ctctctatcg gcaagaaaga attgattgtc gcaacggctg aaggggctct atccctcaaa 840 caagtgcagc cagctggtaa gcctaagatg gacattgctt ccttcctcaa cggagttgga 900 cgtacattga ctgtaggaga acgatttggt gac 933 <210> 192 <211> 311 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (311) <223> Methionyl-tRNA formyltransferase <400> 192 Met Thr Lys Leu Ile Phe Met Gly Thr Pro Asp Phe Ser Ala Thr Val 1 5 10 15 Leu Lys Gly Leu Leu Thr Asp Asp Arg Tyr Glu Ile Leu Ala Val Val 20 25 30 Thr Gln Pro Asp Arg Ala Val Gly Arg Lys Lys Val Ile Gln Glu Thr 35 40 45 Pro Val Lys Gln Ala Ala Lys Glu Ala Gly Leu Ser Ile Tyr Gln Pro 50 55 60 Glu Lys Leu Ser Gly Ser Pro Glu Met Glu Asp Leu Met Lys Leu Gly 65 70 75 80 Ala Asp Gly Ile Val Thr Ala Ala Phe Gly Gln Phe Leu Pro Ser Lys 85 90 95 Leu Leu Asp Ser Met Asp Phe Ala Val Asn Val His Ala Ser Leu Leu 100 105 110 Pro Arg His Arg Gly Gly Ala Pro Ile His Tyr Ala Leu Ile Gln Gly 115 120 125 Asp Glu Glu Ala Gly Val Thr Ile Met Glu Met Val Lys Glu Met Asp 130 135 140 Ala Gly Asp Met Ile Ser Arg Arg Ser Ile Pro Ile Thr Asp Glu Asp 145 150 155 160 Asn Val Gly Thr Leu Phe Glu Lys Leu Ala Leu Val Gly Arg Asp Leu 165 170 175 Leu Leu Asp Thr Leu Pro Ala Tyr Ile Ala Gly Asp Ile Lys Pro Glu 180 185 190 Pro Gln Asp Thr Ser Gln Val Thr Phe Ser Pro Asn Ile Lys Ser Glu 195 200 205 Glu Glu Lys Leu Asn Trp Asn Lys Thr Asn Arg Gln Leu Phe Asn Gln 210 215 220 Ile Arg Gly Met Asn Pro Trp Pro Val Ala His Thr Phe Leu Lys Gly 225 230 235 240 Asp Arg Phe Lys Ile Tyr Glu Ala Leu Pro Val Glu Gly Gln Gly Asn 245 250 255 Pro Gly Glu Ile Leu Ser Ile Gly Lys Lys Glu Leu Ile Val Ala Thr 260 265 270 Ala Glu Gly Ala Leu Ser Leu Lys Gln Val Gln Pro Ala Gly Lys Pro 275 280 285 Lys Met Asp Ile Ala Ser Phe Leu Asn Gly Val Gly Arg Thr Leu Thr 290 295 300 Val Gly Glu Arg Phe Gly Asp 305 310 <210> 193 <211> 570 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (570) <223> Streptococcus pneumoniae spr1603 gene <400> 193 ttgcgtgctc tgaacatttc ccattttgga aggagtacta tgaaacatca atcagccttg 60 gtcgtcttta gtggcggtca agattcaaca acctgccttt tctggaccat gcaacactat 120 gaaacagtcg aagctgtcac ctttgcctac ggccaacatc atcacctcga aattcaaatt 180 actagagaaa tcgctaagga acagggcatt cgtcaccata tcctcgatat gtctctgctg 240 ggacaaatca ctgctcagcc agactttgcg acgattcata tttcctacat tcctgacaag 300 ctctgtgtcg agtcaaaatc cctcaaacta tatctattta gctaccgaaa ccacggagat 360 ttccacgaaa actgtatcaa caccatcggg aaagacttgg tcaacttgct agaccctcgc 420 tatttagaag tctggggaaa attcactccg cgcggtggca tttcaatcga cccctactac 480 aactacggta agcaaggaac taagtatgag ggcttggcag aacaacgcct cttccaacac 540 gacctttatc cagagaaaat tgacaaccgc 570 <210> 194 <211> 190 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (190) <223> Conserved hypothetical protein <400> 194 Met Arg Ala Leu Asn Ile Ser His Phe Gly Arg Ser Thr Met Lys His 1 5 10 15 Gln Ser Ala Leu Val Val Phe Ser Gly Gly Gln Asp Ser Thr Thr Cys 20 25 30 Leu Phe Trp Thr Met Gln His Tyr Glu Thr Val Glu Ala Val Thr Phe 35 40 45 Ala Tyr Gly Gln His His His Leu Glu Ile Gln Ile Thr Arg Glu Ile 50 55 60 Ala Lys Glu Gln Gly Ile Arg His His Ile Leu Asp Met Ser Leu Leu 65 70 75 80 Gly Gln Ile Thr Ala Gln Pro Asp Phe Ala Thr Ile His Ile Ser Tyr 85 90 95 Ile Pro Asp Lys Leu Cys Val Glu Ser Lys Ser Leu Lys Leu Tyr Leu 100 105 110 Phe Ser Tyr Arg Asn His Gly Asp Phe His Glu Asn Cys Ile Asn Thr 115 120 125 Ile Gly Lys Asp Leu Val Asn Leu Leu Asp Pro Arg Tyr Leu Glu Val 130 135 140 Trp Gly Lys Phe Thr Pro Arg Gly Gly Ile Ser Ile Asp Pro Tyr Tyr 145 150 155 160 Asn Tyr Gly Lys Gln Gly Thr Lys Tyr Glu Gly Leu Ala Glu Gln Arg 165 170 175 Leu Phe Gln His Asp Leu Tyr Pro Glu Lys Ile Asp Asn Arg 180 185 190 <210> 195 <211> 1317 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1317) <223> Streptococcus pneumoniae spr1617 gene <400> 195 atgataaata aaacatttac agtagaaaaa gccaatcgtt ttatagcaga aaataaacat 60 ctcgttaata ctcaatataa gcctgaagaa catttttcag ctgagattgg ttggatcaat 120 gatccaaatg gatttgtcta ttttcgtgga gaataccatc tcttttatca attctatcca 180 tatgatagtg tttgggggcc tatgcactgg ggacatgcta aaagtaagga cttggtgact 240 tgggagcact tgccagtggc acttgctcct gatcaagatt atgaccgaaa tggttgtttc 300 tcaggctctg ccattgtcaa ggatgatcgc ctctggctca tgtacactgg gcatatcgaa 360 gaaaaaaccg gtgtccgcca agtgcaaaat atggcatttt cagatgacgg gattcacttt 420 gaaaagattt cccaaaatcc agttgcaact ggatcagact tgccagatga gttgattgct 480 gctgatttcc gtgatccaaa actctttgaa aaagatggac gctattactc cgtagtagct 540 gccaaacaca agaataatgt gggctgtatc gttctactag ggtccgataa cctagtagaa 600 tggcagttcg aatccatctt tttaaaaggg gtagaacacc aaggctttat gtgggaatgc 660 ccagattact tcgagttaga tgggaaagat tgccttatta tgtcacccat gcgttatcag 720 cgtgagggag actcatatca taacatcaac tcatcgcttt tgttcacggg taaggtagat 780 tggggagaaa aatgttttat cccagaatca gttcaagaaa ttgatcatgg ccaagacttc 840 tatgcgcctc aaacattgtt ggacgatcaa aatcgtcgta tcctgattgc ttggatgcag 900 acatgggggc gtacccttcc aacccatgac caagaacaca agtgggcatg tgccatgact 960 ttacctagaa ttctaagatt ggaagatggc aaactaagac aattccctgt taaaaaaggc 1020 caatatcaaa tccaaataga taaagattgt cattaccact taggaaatga tacagattat 1080 cttgaatttg gttatgacag taatgtgcag caagtttaca ttgatcgtag ccatcttatt 1140 caaaaaattc taggtgaaga agaacaggac actagtcgac ggtatgtaga tattgaagct 1200 aaagaattgg aagttgttct agataaaaat tccatcgaga tttttgttaa tcaaggtgaa 1260 gcaagcttga ctgcaactta ttacttaacg gtgccagctg agctatcacg aattgat 1317 <210> 196 <211> 439 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (439) <223> Sucrose-6-phosphate hydrolase <400> 196 Met Ile Asn Lys Thr Phe Thr Val Glu Lys Ala Asn Arg Phe Ile Ala 1 5 10 15 Glu Asn Lys His Leu Val Asn Thr Gln Tyr Lys Pro Glu Glu His Phe 20 25 30 Ser Ala Glu Ile Gly Trp Ile Asn Asp Pro Asn Gly Phe Val Tyr Phe 35 40 45 Arg Gly Glu Tyr His Leu Phe Tyr Gln Phe Tyr Pro Tyr Asp Ser Val 50 55 60 Trp Gly Pro Met His Trp Gly His Ala Lys Ser Lys Asp Leu Val Thr 65 70 75 80 Trp Glu His Leu Pro Val Ala Leu Ala Pro Asp Gln Asp Tyr Asp Arg 85 90 95 Asn Gly Cys Phe Ser Gly Ser Ala Ile Val Lys Asp Asp Arg Leu Trp 100 105 110 Leu Met Tyr Thr Gly His Ile Glu Glu Lys Thr Gly Val Arg Gln Val 115 120 125 Gln Asn Met Ala Phe Ser Asp Asp Gly Ile His Phe Glu Lys Ile Ser 130 135 140 Gln Asn Pro Val Ala Thr Gly Ser Asp Leu Pro Asp Glu Leu Ile Ala 145 150 155 160 Ala Asp Phe Arg Asp Pro Lys Leu Phe Glu Lys Asp Gly Arg Tyr Tyr 165 170 175 Ser Val Val Ala Ala Lys His Lys Asn Asn Val Gly Cys Ile Val Leu 180 185 190 Leu Gly Ser Asp Asn Leu Val Glu Trp Gln Phe Glu Ser Ile Phe Leu 195 200 205 Lys Gly Val Glu His Gln Gly Phe Met Trp Glu Cys Pro Asp Tyr Phe 210 215 220 Glu Leu Asp Gly Lys Asp Cys Leu Ile Met Ser Pro Met Arg Tyr Gln 225 230 235 240 Arg Glu Gly Asp Ser Tyr His Asn Ile Asn Ser Ser Leu Leu Phe Thr 245 250 255 Gly Lys Val Asp Trp Gly Glu Lys Cys Phe Ile Pro Glu Ser Val Gln 260 265 270 Glu Ile Asp His Gly Gln Asp Phe Tyr Ala Pro Gln Thr Leu Leu Asp 275 280 285 Asp Gln Asn Arg Arg Ile Leu Ile Ala Trp Met Gln Thr Trp Gly Arg 290 295 300 Thr Leu Pro Thr His Asp Gln Glu His Lys Trp Ala Cys Ala Met Thr 305 310 315 320 Leu Pro Arg Ile Leu Arg Leu Glu Asp Gly Lys Leu Arg Gln Phe Pro 325 330 335 Val Lys Lys Gly Gln Tyr Gln Ile Gln Ile Asp Lys Asp Cys His Tyr 340 345 350 His Leu Gly Asn Asp Thr Asp Tyr Leu Glu Phe Gly Tyr Asp Ser Asn 355 360 365 Val Gln Gln Val Tyr Ile Asp Arg Ser His Leu Ile Gln Lys Ile Leu 370 375 380 Gly Glu Glu Glu Gln Asp Thr Ser Arg Arg Tyr Val Asp Ile Glu Ala 385 390 395 400 Lys Glu Leu Glu Val Val Leu Asp Lys Asn Ser Ile Glu Ile Phe Val 405 410 415 Asn Gln Gly Glu Ala Ser Leu Thr Ala Thr Tyr Tyr Leu Thr Val Pro 420 425 430 Ala Glu Leu Ser Arg Ile Asp 435 <210> 197 <211> 1221 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) ... (1221) <223> Streptococcus pneumoniae spr1632 gene <400> 197 atggcatatc aagaaccaaa taaagatgga ttttacggaa aatttggcgg acgttttgtc 60 ccagaaacat tgatgacagc agttttggag ttggagaagg cctaccgtga aagtcaggca 120 gacccaagtt tccaagagga attaaaccaa cttttgcgcc agtatgtggg acgtgaaact 180 cccctttact acgcaaaaaa cttgacccag catatcggcg gagccaagat ttatctcaaa 240 cgggaagacc ttaaccatac aggagcccac aagattaaca atgccttagg acaagtttgg 300 cttgccaaac gcatgggtaa aaagaaaatt atcgcagaaa cgggtgctgg tcagcacggt 360 gtggcaactg caactgctgc agccctcttt aatatggaat gtatcatcta catgggtgag 420 gaagatgtca aacgccaagc tctcaatgtc ttccgtatgg agcttttggg agctaaggtc 480 gaggccgtga cagatggttc gcgcgtgctc aaggatgcgg tcaatgcagc ccttcgttca 540 tgggtagcta atatcgatga tacccactat atccttggtt ctaccttggg gcctcatcca 600 tttccagaaa tcgttcgtga cttccaaagt gtcatcggtc gagaggctaa acaacagtac 660 cgtgacttga caggacaaaa tctgccagat gccctagtag cctgtgttgg tggtgggtcg 720 aatgccatcg gtctcttcca tccctttgta gaagatgagt ctgtagctat gtatggggct 780 gaagcagctg gacttggtgt ggatacggag caccacgcag ctaccttgac caagggtcgt 840 ccaggtgtcc ttcacggttc cctcatggat gtgctccaag atgcccatgg tcaaattctt 900 gaagccttct ctatctcagc aggtttggac tatcctggta tcggtccaga gcattctcac 960 taccacgata ttaaacgtgc cagctatgtc cctgtgacag acgaagaagc cttggaagga 1020 ttccaactct tgtctcgtgt ggaagggatt atcccagcct tggaatctag ccatgctatc 1080 gcttttgcgg tgaaattggc caaagaactt ggaccagaaa agtctatgat tgtctgccta 1140 tcaggtcgtg gggacaagga tgtggttcaa gtcaaagacc gcttggaagc agatgcagca 1200 aagaagggag aagctcatgc c 1221 <210> 198 <211> 407 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (407) <223> Tryptophan synthase beta chain <400> 198 Met Ala Tyr Gln Glu Pro Asn Lys Asp Gly Phe Tyr Gly Lys Phe Gly 1 5 10 15 Gly Arg Phe Val Pro Glu Thr Leu Met Thr Ala Val Leu Glu Leu Glu 20 25 30 Lys Ala Tyr Arg Glu Ser Gln Ala Asp Pro Ser Phe Gln Glu Glu Leu 35 40 45 Asn Gln Leu Leu Arg Gln Tyr Val Gly Arg Glu Thr Pro Leu Tyr Tyr 50 55 60 Ala Lys Asn Leu Thr Gln His Ile Gly Gly Ala Lys Ile Tyr Leu Lys 65 70 75 80 Arg Glu Asp Leu Asn His Thr Gly Ala His Lys Ile Asn Asn Ala Leu 85 90 95 Gly Gln Val Trp Leu Ala Lys Arg Met Gly Lys Lys Lys Ile Ila Ala 100 105 110 Glu Thr Gly Ala Gly Gln His Gly Val Ala Thr Ala Thr Ala Ala Ala 115 120 125 Leu Phe Asn Met Glu Cys Ile Ile Tyr Met Gly Glu Glu Asp Val Lys 130 135 140 Arg Gln Ala Leu Asn Val Phe Arg Met Glu Leu Leu Gly Ala Lys Val 145 150 155 160 Glu Ala Val Thr Asp Gly Ser Arg Val Leu Lys Asp Ala Val Asn Ala 165 170 175 Ala Leu Arg Ser Trp Val Ala Asn Ile Asp Asp Thr His Tyr Ile Leu 180 185 190 Gly Ser Thr Leu Gly Pro His Pro Phe Pro Glu Ile Val Arg Asp Phe 195 200 205 Gln Ser Val Ile Gly Arg Glu Ala Lys Gln Gln Tyr Arg Asp Leu Thr 210 215 220 Gly Gln Asn Leu Pro Asp Ala Leu Val Ala Cys Val Gly Gly Gly Ser 225 230 235 240 Asn Ala Ile Gly Leu Phe His Pro Phe Val Glu Asp Glu Ser Val Ala 245 250 255 Met Tyr Gly Ala Glu Ala Ala Gly Leu Gly Val Asp Thr Glu His His 260 265 270 Ala Ala Thr Leu Thr Lys Gly Arg Pro Gly Val Leu His Gly Ser Leu 275 280 285 Met Asp Val Leu Gln Asp Ala His Gly Gln Ile Leu Glu Ala Phe Ser 290 295 300 Ile Ser Ala Gly Leu Asp Tyr Pro Gly Ile Gly Pro Glu His Ser His 305 310 315 320 Tyr His Asp Ile Lys Arg Ala Ser Tyr Val Pro Val Thr Asp Glu Glu 325 330 335 Ala Leu Glu Gly Phe Gln Leu Leu Ser Arg Val Glu Gly Ile Ile Pro 340 345 350 Ala Leu Glu Ser Ser His Ala Ile Ala Phe Ala Val Lys Leu Ala Lys 355 360 365 Glu Leu Gly Pro Glu Lys Ser Met Ile Val Cys Leu Ser Gly Arg Gly 370 375 380 Asp Lys Asp Val Val Gln Val Lys Asp Arg Leu Glu Ala Asp Ala Ala 385 390 395 400 Lys Lys Gly Glu Ala His Ala 405 <210> 199 <211> 153 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (153) <223> Streptococcus pneumoniae spr1642 gene <400> 199 atgcctgcag gctgggattt gctccctctc tctaaaccaa ttatactacc aatgactact 60 gataaaacaa tcctgacaac tatttcaata tttgataacc caagactagt ggctgtcatg 120 attatttcct tactttacgc cccggtcttt gtg 153 <210> 200 <211> 51 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (51) <223> Conserved hypothetical protein <400> 200 Met Pro Ala Gly Trp Asp Leu Leu Pro Leu Ser Lys Pro Ile Ile Leu 1 5 10 15 Pro Met Thr Thr Asp Lys Thr Ile Leu Thr Thr Ile Ser Ile Phe Asp 20 25 30 Asn Pro Arg Leu Val Ala Val Met Ile Ile Ser Leu Leu Tyr Ala Pro 35 40 45 Val Phe Val 50 <210> 201 <211> 978 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (978) <223> Streptococcus pneumoniae spr1647 gene <400> 201 atggcaatat tggtaacagg cggagctggt tatattggta gccataccgt agtagaatta 60 ctaaatttag gaaaggaagt catcattgtc gacaaccttt cgaactctag catcttggtg 120 ctagaccgta ttgaagcaat tacaggaata cgtcccgtgt tttacgaatt agatgtttgt 180 gataaaccag cattgagaaa ggtatttgaa caagaatcga ttgatgctgc aattcatttt 240 gcaggttata aagctgtcgg agaatccgtg caaaagcctg tgatgtacta caaaaataat 300 attatgagta cattggcact tgttgaagtg atgtcagaat ttaatgttaa aaagattgtc 360 ttttcttcaa gtgcgactgt atatggaatt aacaatcagt cacctctaat tgagactatg 420 caaacaagtg cgacaaatcc ttatgggtat acgaaagtga tgcttgagca aattttaaaa 480 gatgttcatg tggcagattc agaatggagt attgcgttgc ttcgttattt caatccaatt 540 ggtgctcatg agtctggttt gattggggaa gatccctcag gaattcctaa caacttgatg 600 ccctatattg cacaagtagc ggtaggtaag ctgtcagaac taagtgtgtt cggaaatgat 660 tatgatacgc ttgacggtac tggtgtgcgc gattatatcc atgtagtaga tttagcaata 720 ggacatataa aagctttaga aaaagtatct gaaaaaacag atgtttatat ctataaccta 780 ggctcgggag aaggcactag tgtattacaa cttgtaaata catttgaaag tgttaataag 840 atccctattc cttataaaat agttccaaga cgttcaggag acgttgcaac ttgttatgca 900 aatgcagaca aagcatataa ggaattaaat tggaggacaa caaaatcgat tgaagacatg 960 tgtagagata catggaat 978 <210> 202 <211> 326 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (326) UDP-glucose 4-epimerase <400> 202 Met Ala Ile Leu Val Thr Gly Gly Ala Gly Tyr Ile Gly Ser His Thr 1 5 10 15 Val Val Glu Leu Leu Asn Leu Gly Lys Glu Val Ile Ile Val Asp Asn 20 25 30 Leu Ser Asn Ser Ser Ile Leu Val Leu Asp Arg Ile Glu Ala Ile Thr 35 40 45 Gly Ile Arg Pro Val Phe Tyr Glu Leu Asp Val Cys Asp Lys Pro Ala 50 55 60 Leu Arg Lys Val Phe Glu Gln Glu Ser Ile Asp Ala Ala Ile His Phe 65 70 75 80 Ala Gly Tyr Lys Ala Val Gly Glu Ser Val Gln Lys Pro Val Met Tyr 85 90 95 Tyr Lys Asn Asn Ile Met Ser Thr Leu Ala Leu Val Glu Val Met Ser 100 105 110 Glu Phe Asn Val Lys Lys Ile Val Phe Ser Ser Ser Ala Thr Val Tyr 115 120 125 Gly Ile Asn Asn Gln Ser Pro Leu Ile Glu Thr Met Gln Thr Ser Ala 130 135 140 Thr Asn Pro Tyr Gly Tyr Thr Lys Val Met Leu Glu Gln Ile Leu Lys 145 150 155 160 Asp Val His Val Ala Asp Ser Glu Trp Ser Ile Ala Leu Leu Arg Tyr 165 170 175 Phe Asn Pro Ile Gly Ala His Glu Ser Gly Leu Ile Gly Glu Asp Pro 180 185 190 Ser Gly Ile Pro Asn Asn Leu Met Pro Tyr Ile Ala Gln Val Ala Val 195 200 205 Gly Lys Leu Ser Glu Leu Ser Val Phe Gly Asn Asp Tyr Asp Thr Leu 210 215 220 Asp Gly Thr Gly Val Arg Asp Tyr Ile His Val Val Asp Leu Ala Ile 225 230 235 240 Gly His Ile Lys Ala Leu Glu Lys Val Ser Glu Lys Thr Asp Val Tyr 245 250 255 Ile Tyr Asn Leu Gly Ser Gly Glu Gly Thr Ser Val Leu Gln Leu Val 260 265 270 Asn Thr Phe Glu Ser Val Asn Lys Ile Pro Ile Pro Tyr Lys Ile Val 275 280 285 Pro Arg Arg Ser Gly Asp Val Ala Thr Cys Tyr Ala Asn Ala Asp Lys 290 295 300 Ala Tyr Lys Glu Leu Asn Trp Arg Thr Thr Lys Ser Ile Glu Asp Met 305 310 315 320 Cys Arg Asp Thr Trp Asn 325 <210> 203 <211> 423 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene <222> (1) .. (423) <223> Streptococcus pneumoniae spr1666 gene <400> 203 ttgaaatatc aggaggaaac gatgaaagac tttcattttg acgctatatc tgcctttgaa 60 aattacgaaa ttgaaaaaat gagagatggt cacgttgtgg tgacgaccaa agtagtgaac 120 tcatcgctca actactatgg caatgcccat ggtggctatc tattcacgct atgtgaccaa 180 atcagtggtt tggtggttat ctcgctggga cttgatgggg tgacactcca atcctctatc 240 aactacctta aggcaggaaa actcgacgat gtattgacca ttaaaggaga atgtgtccat 300 caaggtcgta caacctgtgt gatggatgtg gatatcacca atcaagaggg cagaaatgtc 360 tgtaaggcaa cctttaccat gtttgtcaca ggccaaaggt ctgaggaaag acgggtaagt 420 ata 423 <210> 204 <211> 141 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (141) <223> Conserved hypothetical protein <400> 204 Met Lys Tyr Gln Glu Glu Thr Met Lys Asp Phe His Phe Asp Ala Ile 1 5 10 15 Ser Ala Phe Glu Asn Tyr Glu Ile Glu Lys Met Arg Asp Gly His Val 20 25 30 Val Val Thr Thr Lys Val Val Asn Ser Ser Leu Asn Tyr Tyr Gly Asn 35 40 45 Ala His Gly Gly Tyr Leu Phe Thr Leu Cys Asp Gln Ile Ser Gly Leu 50 55 60 Val Val Ile Ser Leu Gly Leu Asp Gly Val Thr Leu Gln Ser Ser Ile 65 70 75 80 Asn Tyr Leu Lys Ala Gly Lys Leu Asp Asp Val Leu Thr Ile Lys Gly 85 90 95 Glu Cys Val His Gln Gly Arg Thr Thr Cys Val Met Asp Val Asp Ile 100 105 110 Thr Asn Gln Glu Gly Arg Asn Val Cys Lys Ala Thr Phe Thr Met Phe 115 120 125 Val Thr Gly Gln Arg Ser Glu Glu Arg Arg Val Ser Ile 130 135 140 <210> 205 <211> 351 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (351) <223> Streptococcus pneumoniae spr1671 gene <400> 205 gtgaatatta aatctgccag tgatttgttg ggaatttcag cggatacgat tcggtattat 60 gaacgggttg gtcttgtgcc accgattact cgtactgcta ctgggattcg tgattttcaa 120 gatcaggata tcgaagcgct ggaatttatt aagtgttttc gttcggcggg tgtctctgta 180 gatagtttag ttgactatat gtcgctctac caaaagggag atgaaacgag agaggagagg 240 cttggtattt tagaagagga aaagcaaaaa ttagaggagc gcttgtctca gctacagaca 300 gctttaaatc gtttaaatct caaaattaaa ctttataagg aaggaaaatt t 351 <206> 206 <211> 117 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (117) <223> Conserved hypothetical protein <400> 206 Met Asn Ile Lys Ser Ala Ser Asp Leu Leu Gly Ile Ser Ala Asp Thr 1 5 10 15 Ile Arg Tyr Tyr Glu Arg Val Gly Leu Val Pro Pro Ile Thr Arg Thr 20 25 30 Ala Thr Gly Ile Arg Asp Phe Gln Asp Gln Asp Ile Glu Ala Leu Glu 35 40 45 Phe Ile Lys Cys Phe Arg Ser Ala Gly Val Ser Val Asp Ser Leu Val 50 55 60 Asp Tyr Met Ser Leu Tyr Gln Lys Gly Asp Glu Thr Arg Glu Glu Arg 65 70 75 80 Leu Gly Ile Leu Glu Glu Glu Lys Gln Lys Leu Glu Glu Arg Leu Ser 85 90 95 Gln Leu Gln Thr Ala Leu Asn Arg Leu Asn Leu Lys Ile Lys Leu Tyr 100 105 110 Lys Glu Gly Lys Phe 115 <210> 207 <211> 807 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (807) <223> Streptococcus pneumoniae spr1674 gene <400> 207 atgatgcata cttatttgca aaagaaaatt gaaaatatca aaacaaccct aggtgaaatg 60 tcaggtggtt accgtcgtat ggttgcggct atggctgatt taggattttc aggaactatg 120 aaggctatct gggatgacct ctttgcccat cgtagttttg cccagtggat ttatttgctg 180 gttttaggaa gttttcctct ctggctggag ttggtttacg aacatcgtat tgttgactgg 240 attgggatga tttgtagctt gacagggatt atctgtgtaa tctttgtatc ggaaggtcga 300 gcaagtaatt atctttttgg cttgattaac tctgttattt accttatttt ggccctacag 360 aaaggctttt atggtgaggt gctgacgaca ctttacttca cagtcatgca gccaattgga 420 cttctagttt ggatttatca ggcacagttt aagaaggaaa agcaggagtt tgtcgcgcgt 480 aaactggacg gcaagggctg gacaaagtat ctttccatta gtgtgctttg gtggttggcc 540 tttggcttca tttatcagtc tattggtgcc aatcgtccct atcgtgattc aatcacagat 600 gcaaccaatg gggtagggca aatcctcatg acagctgttt accgtgaaca gtggatattc 660 tgggcggcta ccaatgtctt ttcaatctat ctctggtggg gagaaagcct gcaaattcaa 720 gggaaatatc taatttatct cattaacagt ctagttggtt ggtatcaatg gagcaaggca 780 gctaagcaga atactgattt acttaac 807 <210> 208 <211> 269 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (269) <223> Conserved hypothetical protein <400> 208 Met Met His Thr Tyr Leu Gln Lys Lys Ile Glu Asn Ile Lys Thr Thr 1 5 10 15 Leu Gly Glu Met Ser Gly Gly Tyr Arg Arg Met Val Ala Ala Met Ala 20 25 30 Asp Leu Gly Phe Ser Gly Thr Met Lys Ala Ile Trp Asp Asp Leu Phe 35 40 45 Ala His Arg Ser Phe Ala Gln Trp Ile Tyr Leu Leu Val Leu Gly Ser 50 55 60 Phe Pro Leu Trp Leu Glu Leu Val Tyr Glu His Arg Ile Val Asp Trp 65 70 75 80 Ile Gly Met Ile Cys Ser Leu Thr Gly Ile Ile Cys Val Ile Phe Val 85 90 95 Ser Glu Gly Arg Ala Ser Asn Tyr Leu Phe Gly Leu Ile Asn Ser Val 100 105 110 Ile Tyr Leu Ile Leu Ala Leu Gln Lys Gly Phe Tyr Gly Glu Val Leu 115 120 125 Thr Thr Leu Tyr Phe Thr Val Met Gln Pro Ile Gly Leu Leu Val Trp 130 135 140 Ile Tyr Gln Ala Gln Phe Lys Lys Glu Lys Gln Glu Phe Val Ala Arg 145 150 155 160 Lys Leu Asp Gly Lys Gly Trp Thr Lys Tyr Leu Ser Ile Ser Val Leu 165 170 175 Trp Trp Leu Ala Phe Gly Phe Ile Tyr Gln Ser Ile Gly Ala Asn Arg 180 185 190 Pro Tyr Arg Asp Ser Ile Thr Asp Ala Thr Asn Gly Val Gly Gln Ile 195 200 205 Leu Met Thr Ala Val Tyr Arg Glu Gln Trp Ile Phe Trp Ala Ala Thr 210 215 220 Asn Val Phe Ser Ile Tyr Leu Trp Trp Gly Glu Ser Leu Gln Ile Gln 225 230 235 240 Gly Lys Tyr Leu Ile Tyr Leu Ile Asn Ser Leu Val Gly Trp Tyr Gln 245 250 255 Trp Ser Lys Ala Ala Lys Gln Asn Thr Asp Leu Leu Asn 260 265 <210> 209 <211> 792 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (792) <223> Streptococcus pneumoniae spr1696 gene <400> 209 atggataatc gaccaattgg ttttttggat tcgggtgtcg ggggcttgac cgttgtgcgc 60 gagctcatgc gccagcttcc ccatgaagaa atcgtctata ttggagattc ggcgcgggcg 120 ccctatggcc cccgtcctgc tgagcaaatt cgtgaatata cttggcagct ggtcaacttt 180 ctcttgacca aggatgtcaa aatgattgtc attgcttgta acactgcgac tgcggttgtc 240 tgggaagaaa tcaaggctca actagatatt cctgtcttgg gtgtaatttt gccaggagct 300 tcggcagcca tcaagtccag tcaaggtggg aaaatcggag tgattggaac gcccatgacg 360 gtacaatcag acatataccg tcagaaaatc catgatctgg atcccgactt acaggtggag 420 agcttggcct gtcccaagtt tgctcccttg gttgagtcag gtgccctgtc aaccagtgtt 480 accaagaagg tggtctatga aaccctgcgt cccttggttg gaaaggtgga tagcctgatt 540 ttgggctgta ctcattatcc actccttcgc cctattatcc aaaatgtgat ggggccaaag 600 gttcagctca tcgatagtgg ggcagagtgc gtacgggata tttcagtctt actcaattat 660 tttgaaatca atcgtggtcg cgatgctgga ccactccatc accgttttta cacaacagcc 720 agtagccaaa gttttgcaca aattggtgaa gaatggctgg aaaaagagat tcatgtggag 780 catgtagaat ta 792 <210> 210 <211> 264 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN <222> (1) .. (264) <223> Glutamate racemase <400> 210 Met Asp Asn Arg Pro Ile Gly Phe Leu Asp Ser Gly Val Gly Gly Leu 1 5 10 15 Thr Val Val Arg Glu Leu Met Arg Gln Leu Pro His Glu Glu Ile Val 20 25 30 Tyr Ile Gly Asp Ser Ala Arg Ala Pro Tyr Gly Pro Arg Pro Ala Glu 35 40 45 Gln Ile Arg Glu Tyr Thr Trp Gln Leu Val Asn Phe Leu Leu Thr Lys 50 55 60 Asp Val Lys Met Ile Val Ile Ala Cys Asn Thr Ala Thr Ala Val Val 65 70 75 80 Trp Glu Glu Ile Lys Ala Gln Leu Asp Ile Pro Val Leu Gly Val Ile 85 90 95 Leu Pro Gly Ala Ser Ala Ala Ile Lys Ser Ser Gln Gly Gly Lys Ile 100 105 110 Gly Val Ile Gly Thr Pro Met Thr Val Gln Ser Asp Ile Tyr Arg Gln 115 120 125 Lys Ile His Asp Leu Asp Pro Asp Leu Gln Val Glu Ser Leu Ala Cys 130 135 140 Pro Lys Phe Ala Pro Leu Val Glu Ser Gly Ala Leu Ser Thr Ser Val 145 150 155 160 Thr Lys Lys Val Val Tyr Glu Thr Leu Arg Pro Leu Val Gly Lys Val 165 170 175 Asp Ser Leu Ile Leu Gly Cys Thr His Tyr Pro Leu Leu Arg Pro Ile 180 185 190 Ile Gln Asn Val Met Gly Pro Lys Val Gln Leu Ile Asp Ser Gly Ala 195 200 205 Glu Cys Val Arg Asp Ile Ser Val Leu Leu Asn Tyr Phe Glu Ile Asn 210 215 220 Arg Gly Arg Asp Ala Gly Pro Leu His His Arg Phe Tyr Thr Thr Ala 225 230 235 240 Ser Ser Gln Ser Phe Ala Gln Ile Gly Glu Glu Trp Leu Glu Lys Glu 245 250 255 Ile His Val Glu His Val Glu Leu 260 <210> 211 <211> 300 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (300) <223> Streptococcus pneumoniae spr1697 gene <400> 211 gtggctagtt tgtggtataa tgaaagggac tcaaaaagaa acaggagaaa aatgatggat 60 ttacttttag caattgtatt gattgtgcta gcttttctag gaggagctct tggaggaatg 120 tacttggttc gtaagcaaat cgaaaaagaa tttgctgaca acccacgttt gaatgctgaa 180 gcagttcgta ctcttttgag tgcaaatggt caaaaaccaa gcgaagctaa ggtacaacaa 240 gtttaccacc aaatcatccg ccaacaaaag gcagcccttg ctaacaataa aaagaaaaaa 300 300 <210> 212 <211> 100 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (100) <223> Conserved hypothetical protein <400> 212 Met Ala Ser Leu Trp Tyr Asn Glu Arg Asp Ser Lys Arg Asn Arg Arg 1 5 10 15 Lys Met Met Asp Leu Leu Leu Ala Ile Val Leu Ile Val Leu Ala Phe 20 25 30 Leu Gly Gly Ala Leu Gly Gly Met Tyr Leu Val Arg Lys Gln Ile Glu 35 40 45 Lys Glu Phe Ala Asp Asn Pro Arg Leu Asn Ala Glu Ala Val Arg Thr 50 55 60 Leu Leu Ser Ala Asn Gly Gln Lys Pro Ser Glu Ala Lys Val Gln Gln 65 70 75 80 Val Tyr His Gln Ile Ile Arg Gln Gln Lys Ala Ala Leu Ala Asn Asn 85 90 95 Lys Lys Lys Lys 100 <210> 213 <211> 3675 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (3675) <223> Streptococcus pneumoniae spr1776 gene <400> 213 gtggttgatg taaatcgttt taaaagtatg caaatcaccc tagcttctcc aagtaaagtc 60 cgttcatggt cttatggaga agtcaaaaaa cctgaaacaa tcaattatcg taccttgaaa 120 ccagaacgtg aaggactctt tgatgaagtg atctttggtc ctacaaaaga ctgggaatgt 180 gcttgtggta agtacaaacg cattcgttac agaggaattg tttgtgaccg ctgtggggtt 240 gaagtaacgc gtacgaaagt tcgtcgtgag cgtatgggac atatcgaatt gaaagctcct 300 gtatctcaca tctggtactt caaggggatt ccaagccgta tgggcttgac ccttgatatg 360 agccctcgtg ccctcgagga agttatctac tttgcggctt atgtggtgat tgatcctaag 420 gatacaccac ttgagcacaa gtctatcatg acagagcgcg aataccgaga gcgcttgcgc 480 gaatatggtt atggttcatt tgttgctaag atgggtgcgg aagccatcca agaccttttg 540 aagcaagtag atcttgaaaa agaaattgct gaactcaaag aagaattgaa aactgctact 600 ggacaaaaac gtgtcaaagc catccgtcgt ttggatgttt tggatgcctt ttacaagtct 660 ggaaacaaac ctgaatggat gattcttaac atccttccgg ttatcccacc agatcttcgt 720 ccaatgttgc agttggatgg tggccgtttt gcctcatctg acttgaatga cctttaccgc 780 cgtgttatca accgtaacaa ccgtttggct cgtttgcttg agttaaatgc accaggtatc 840 atcgttcaaa atgagaagcg tatgcttcaa gaagcagttg acgctttgat tgacaatggt 900 cgtcgtggtc gtccaatcac aggaccaggt agccgtccat tgaaatcatt gagccacatg 960 cttaaaggta aacaaggacg cttccgtcaa aacttgctcg gtaaacgtgt tgacttctca 1020 ggacgttccg ttatcgccgt tggtccaact cttaagatgt accaatgtgg tgtgccacgt 1080 gaaatggcga ttgaactctt taaaccattt gtcatgcgtg aaatcgttgc ccgtgatatc 1140 gtgcaaaacg tcaaagcagc taaacgcttg gtggaacgcg gagatgagcg tatctgggat 1200 atccttgaag aagtgattaa agaacaccca gtgcttttga accgcgcacc gacccttcac 1260 cgtttgggta tccaagcctt cgagccagtc ttgattgatg gtaaggctct tcgcttgcac 1320 ccacttgtct gtgaagccta caatgctgac tttgacgggg accaaatggc catccacgta 1380 ccactttcag aagaagcaca agcagaagct cgtatcctca tgctagctgc tgagcacatc 1440 ttgaacccga aagatgggaa accggtagtt actccatctc aggacatggt tttgggtaac 1500 tactacttga ccatggaaga agctggtcgc gaaggtgaag gaatggtctt caaagaccgt 1560 gacgaagcgg ttatggctta ccgcaatggt tatgttcacc tccactcacg tgttggtatc 1620 gcaacagaca gcctcaacaa gccttggaca gaagagcaaa gacataaggt cttgcttaca 1680 acagttggta aaattctctt caacgatatc atgccagagg ggctaccata cttgcaagaa 1740 ccaaacaatg ccaacttgac agaaggtgtt ccagctaaat acttcttgcc acttggtgga 1800 gctatcaagg aagctatcag caatcttgag ctcaaccctc cattcaagaa gaaaaacctc 1860 ggaaatatca tcgctgaaat cttcaaacgt ttccgtacga cagaaacttc tgccctactt 1920 gaccgcatga agaacctcgg ttaccaccac tcaactcttg caggattgac agtgggtatt 1980 gccgatatcc cagtcgttga tgacaaggct gaaatcattg aagaatcaca caaacgtgtc 2040 gaacaaatca ccaaacaatt ccgtcgtggt atgatcacag acgacgagcg ttacaatgct 2100 gttacagctg aatggcgtgc tgcccgtgaa aaacttgaga aacgcttgat tgctaatcaa 2160 gatcctaaga acccaatcgt tatgatgatg gactctggag cccgtggtaa catctcaaac 2220 ttctcacagc ttgccggtat gcgtggtctg atggctgctc cgaatggacg tatcatggaa 2280 ttgccaatcc tttcaaactt ccgcgaaggt ttgtcggtat tggaaatgtt cttctcaact 2340 cacggtgctc gtaaaggtat gaccgatacg gcccttaaga cagccgactc aggttacttg 2400 actcgtcgtt tggtcgacgt tgcccaagac gttatcatcc gtgaggacga ctgtggaact 2460 gaccgtggtc tcttgatccg ttctatcgca gaaggaaaag agatgatcga gtctctcgaa 2520 gagcgtctca acggtcgtta cactaagaaa actgttaaac atccagaaac tggtgcagtg 2580 attattggtc caaatgaatt gattacagaa gacaaggcgc gtgaaattgt caatgctggt 2640 gtggaagaag tgactatccg ttctgtattt acatgtaaca ctcgtcgcgg tgtctgccgt 2700 cactgttacg gtatcaactt ggcgactggt gatgcggttg aagttggtga agcagttggt 2760 acaatcgctg cccaatctat cggggaacct ggtacacagc ttacaatgcg taccttccac 2820 acaggtgggg ttgcctcaaa taccgatatc actcagggtc ttcctcgtgt ccaagaaatc 2880 tttgaagccc gcaatcctaa aggggaagcg gttattacag aggttaaagg acaagttact 2940 gctatcgaag aagatgcatc aactcgtacc aagaaagtct ttgttaaggg tgaaactggc 3000 gaaggtgaat atgtcgttcc atttacagct cgtatgcgtg tcgaagttgg gggccaagta 3060 gcgcgtggtg ctgctctgac agaaggttct atccaaccaa aacgtctcct tgcagttcgt 3120 gatgtcttgt cagttgaaac gtaccttctc ggtgaagtac aaaaagttta ccgtagccaa 3180 ggggtagaaa tcggtgacaa acacatcgag gtaatggttc gtcaaatgat ccgtaaagtc 3240 cgtgtcatgg atccaggtga tacagatctt ctcatgggta ccctcatgga tatcaatgac 3300 tttacagatg ccaacaaaga tgtccttatc gcaggtggag ttccagcgac aggtcgccca 3360 gtccttatgg gaattaccaa agcctcactt gaaacaaaca gtttcttgtc agcggcttcc 3420 ttccaggaaa caactcgtgt ccttactgac gcagctatcc gtggtaagaa agaccatctc 3480 cttggactta aagaaaatgt tatcatcggt aagatcatcc cagctggtac tggtatggcc 3540 cgttaccgta accttgaacc acatgctgtc aacgaagaag aataccttaa tcctccagta 3600 gaggaagaag gaaatgaaga aacaacagaa gtagttgtgg atactgccgt tgaaactgtg 3660 gaagaaacag tagaa 3675 <210> 214 <211> 1225 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (1225) <223> DNA-dependent RNA polymerase <400> 214 Met Val Asp Val Asn Arg Phe Lys Ser Met Gln Ile Thr Leu Ala Ser 1 5 10 15 Pro Ser Lys Val Arg Ser Trp Ser Tyr Gly Glu Val Lys Lys Pro Glu 20 25 30 Thr Ile Asn Tyr Arg Thr Leu Lys Pro Glu Arg Glu Gly Leu Phe Asp 35 40 45 Glu Val Ile Phe Gly Pro Thr Lys Asp Trp Glu Cys Ala Cys Gly Lys 50 55 60 Tyr Lys Arg Ile Arg Tyr Arg Gly Ile Val Cys Asp Arg Cys Gly Val 65 70 75 80 Glu Val Thr Arg Thr Lys Val Arg Arg Glu Arg Met Gly His Ile Glu 85 90 95 Leu Lys Ala Pro Val Ser His Ile Trp Tyr Phe Lys Gly Ile Pro Ser 100 105 110 Arg Met Gly Leu Thr Leu Asp Met Ser Pro Arg Ala Leu Glu Glu Val 115 120 125 Ile Tyr Phe Ala Ala Tyr Val Val Ile Asp Pro Lys Asp Thr Pro Leu 130 135 140 Glu His Lys Ser Ile Met Thr Glu Arg Glu Tyr Arg Glu Arg Leu Arg 145 150 155 160 Glu Tyr Gly Tyr Gly Ser Phe Val Ala Lys Met Gly Ala Glu Ala Ile 165 170 175 Gln Asp Leu Leu Lys Gln Val Asp Leu Glu Lys Glu Ile Ala Glu Leu 180 185 190 Lys Glu Glu Leu Lys Thr Ala Thr Gly Gln Lys Arg Val Lys Ala Ile 195 200 205 Arg Arg Leu Asp Val Leu Asp Ala Phe Tyr Lys Ser Gly Asn Lys Pro 210 215 220 Glu Trp Met Ile Leu Asn Ile Leu Pro Val Ile Pro Pro Asp Leu Arg 225 230 235 240 Pro Met Leu Gln Leu Asp Gly Gly Arg Phe Ala Ser Ser Asp Leu Asn 245 250 255 Asp Leu Tyr Arg Arg Val Ile Asn Arg Asn Asn Arg Leu Ala Arg Leu 260 265 270 Leu Glu Leu Asn Ala Pro Gly Ile Ile Val Gln Asn Glu Lys Arg Met 275 280 285 Leu Gln Glu Ala Val Asp Ala Leu Ile Asp Asn Gly Arg Arg Gly Arg 290 295 300 Pro Ile Thr Gly Pro Gly Ser Arg Pro Leu Lys Ser Leu Ser His Met 305 310 315 320 Leu Lys Gly Lys Gln Gly Arg Phe Arg Gln Asn Leu Leu Gly Lys Arg 325 330 335 Val Asp Phe Ser Gly Arg Ser Val Ile Ala Val Gly Pro Thr Leu Lys 340 345 350 Met Tyr Gln Cys Gly Val Pro Arg Glu Met Ala Ile Glu Leu Phe Lys 355 360 365 Pro Phe Val Met Arg Glu Ile Val Ala Arg Asp Ile Val Gln Asn Val 370 375 380 Lys Ala Ala Lys Arg Leu Val Glu Arg Gly Asp Glu Arg Ile Trp Asp 385 390 395 400 Ile Leu Glu Glu Val Ile Lys Glu His Pro Val Leu Leu Asn Arg Ala 405 410 415 Pro Thr Leu His Arg Leu Gly Ile Gln Ala Phe Glu Pro Val Leu Ile 420 425 430 Asp Gly Lys Ala Leu Arg Leu His Pro Leu Val Cys Glu Ala Tyr Asn 435 440 445 Ala Asp Phe Asp Gly Asp Gln Met Ala Ile His Val Pro Leu Ser Glu 450 455 460 Glu Ala Gln Ala Glu Ala Arg Ile Leu Met Leu Ala Ala Glu His Ile 465 470 475 480 Leu Asn Pro Lys Asp Gly Lys Pro Val Val Thr Pro Ser Gln Asp Met 485 490 495 Val Leu Gly Asn Tyr Tyr Leu Thr Met Glu Glu Ala Gly Arg Glu Gly 500 505 510 Glu Gly Met Val Phe Lys Asp Arg Asp Glu Ala Val Met Ala Tyr Arg 515 520 525 Asn Gly Tyr Val His Leu His Ser Arg Val Gly Ile Ala Thr Asp Ser 530 535 540 Leu Asn Lys Pro Trp Thr Glu Glu Gln Arg His Lys Val Leu Leu Thr 545 550 555 560 Thr Val Gly Lys Ile Leu Phe Asn Asp Ile Met Pro Glu Gly Leu Pro 565 570 575 Tyr Leu Gln Glu Pro Asn Asn Ala Asn Leu Thr Glu Gly Val Pro Ala 580 585 590 Lys Tyr Phe Leu Pro Leu Gly Gly Ala Ile Lys Glu Ala Ile Ser Asn 595 600 605 Leu Glu Leu Asn Pro Pro Phe Lys Lys Lys Asn Leu Gly Asn Ile Ile 610 615 620 Ala Glu Ile Phe Lys Arg Phe Arg Thr Thr Glu Thr Ser Ala Leu Leu 625 630 635 640 Asp Arg Met Lys Asn Leu Gly Tyr His His Ser Thr Leu Ala Gly Leu 645 650 655 Thr Val Gly Ile Ala Asp Ile Pro Val Val Asp Asp Lys Ala Glu Ile 660 665 670 Ile Glu Glu Ser His Lys Arg Val Glu Gln Ile Thr Lys Gln Phe Arg 675 680 685 Arg Gly Met Ile Thr Asp Asp Glu Arg Tyr Asn Ala Val Thr Ala Glu 690 695 700 Trp Arg Ala Ala Arg Glu Lys Leu Glu Lys Arg Leu Ile Ala Asn Gln 705 710 715 720 Asp Pro Lys Asn Pro Ile Val Met Met Met Asp Ser Gly Ala Arg Gly 725 730 735 Asn Ile Ser Asn Phe Ser Gln Leu Ala Gly Met Arg Gly Leu Met Ala 740 745 750 Ala Pro Asn Gly Arg Ile Met Glu Leu Pro Ile Leu Ser Asn Phe Arg 755 760 765 Glu Gly Leu Ser Val Leu Glu Met Phe Phe Ser Thr His Gly Ala Arg 770 775 780 Lys Gly Met Thr Asp Thr Ala Leu Lys Thr Ala Asp Ser Gly Tyr Leu 785 790 795 800 Thr Arg Arg Leu Val Asp Val Ala Gln Asp Val Ile Arle Glu Asp 805 810 815 Asp Cys Gly Thr Asp Arg Gly Leu Leu Ile Arg Ser Ile Ala Glu Gly 820 825 830 Lys Glu Met Ile Glu Ser Leu Glu Glu Arg Leu Asn Gly Arg Tyr Thr 835 840 845 Lys Lys Thr Val Lys His Pro Glu Thr Gly Ala Val Ile Gly Pro 850 855 860 Asn Glu Leu Ile Thr Glu Asp Lys Ala Arg Glu Ile Val Asn Ala Gly 865 870 875 880 Val Glu Glu Val Thr Ile Arg Ser Val Phe Thr Cys Asn Thr Arg Arg 885 890 895 Gly Val Cys Arg His Cys Tyr Gly Ile Asn Leu Ala Thr Gly Asp Ala 900 905 910 Val Glu Val Gly Glu Ala Val Gly Thr Ile Ala Ala Gln Ser Ile Gly 915 920 925 Glu Pro Gly Thr Gln Leu Thr Met Arg Thr Phe His Thr Gly Gly Val 930 935 940 Ala Ser Asn Thr Asp Ile Thr Gln Gly Leu Pro Arg Val Gln Glu Ile 945 950 955 960 Phe Glu Ala Arg Asn Pro Lys Gly Glu Ala Val Ile Thr Glu Val Lys 965 970 975 Gly Gln Val Thr Ala Ile Glu Glu Asp Ala Ser Thr Arg Thr Lys Lys 980 985 990 Val Phe Val Lys Gly Glu Thr Gly Glu Gly Glu Tyr Val Val Pro Phe 995 1000 1005 Thr Ala Arg Met Arg Val Glu Val Gly Gly Gln Val Ala Arg Gly Ala 1010 1015 1020 Ala Leu Thr Glu Gly Ser Ile Gln Pro Lys Arg Leu Leu Ala Val Arg 1025 1030 1035 1040 Asp Val Leu Ser Val Glu Thr Tyr Leu Leu Gly Glu Val Gln Lys Val 1045 1050 1055 Tyr Arg Ser Gln Gly Val Glu Ile Gly Asp Lys His Ile Glu Val Met 1060 1065 1070 Val Arg Gln Met Ile Arg Lys Val Arg Val Met Asp Pro Gly Asp Thr 1075 1080 1085 Asp Leu Leu Met Gly Thr Leu Met Asp Ile Asn Asp Phe Thr Asp Ala 1090 1095 1100 Asn Lys Asp Val Leu Ile Ala Gly Gly Val Pro Ala Thr Gly Arg Pro 1105 1110 1115 1120 Val Leu Met Gly Ile Thr Lys Ala Ser Leu Glu Thr Asn Ser Phe Leu 1125 1130 1135 Ser Ala Ala Ser Phe Gln Glu Thr Thr Arg Val Leu Thr Asp Ala Ala 1140 1145 1150 Ile Arg Gly Lys Lys Asp His Leu Leu Gly Leu Lys Glu Asn Val Ile 1155 1160 1165 Ile Gly Lys Ile Ile Pro Ala Gly Thr Gly Met Ala Arg Tyr Arg Asn 1170 1175 1180 Leu Glu Pro His Ala Val Asn Glu Glu Glu Tyr Leu Asn Pro Pro Val 1185 1190 1195 1200 Glu Glu Glu Gly Asn Glu Glu Thr Thr Glu Val Val Val Asp Thr Ala 1205 1210 1215 Val Glu Thr Val Glu Glu Thr Val Glu 1220 1225 <210> 215 <211> 654 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (654) <223> Streptococcus pneumoniae spr1797 gene <400> 215 atgtctcaat acaagattgc tccgtcaatt ctggcagcag attatgccaa ctttgaacgt 60 gaaatcaaac gtctagaagc aactggggca gaatatgccc atatcgatat catggacagt 120 cattttgtac cgcaaatcag ttttggtgca ggtgtggtcg agagccttcg tcctcatagt 180 aagatggttt tcgattgcca cttgatggtg tcaaaccctg agcatcatct ggaagatttt 240 gcgcgtgcag gtgcagacat catcagtatc catgtagaag caacacctca tattcatggc 300 gccctccaaa aaattcgttc actcggagtt aagccttcag tcgttatcaa tcctggcaca 360 ccagttgaag ccatcaagca cgtccttcat ctagttgacc aagttttagt catgacggtt 420 aatccaggtt ttggtgggca agcctttctg ccagaaacca tggataaggt tcgtgagttg 480 gttgctcttc gtgaggaaaa aggtttgaac tttgaaatcg aagtggatgg tgggattgat 540 gaccaaacta ttgctcaagc caaagaagct ggtgcgactg tttttgtagc aggttcctat 600 gtctttaagg gagaagtcaa tgagcgagta caaactctca gaaaacaact ggac 654 <210> 216 <211> 218 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (218) <223> Pentose-5-phosphate-3-epimerase <400> 216 Met Ser Gln Tyr Lys Ile Ala Pro Ser Ile Leu Ala Ala Asp Tyr Ala 1 5 10 15 Asn Phe Glu Arg Glu Ile Lys Arg Leu Glu Ala Thr Gly Ala Glu Tyr 20 25 30 Ala His Ile Asp Ile Met Asp Ser His Phe Val Pro Gln Ile Ser Phe 35 40 45 Gly Ala Gly Val Val Glu Ser Leu Arg Pro His Ser Lys Met Val Phe 50 55 60 Asp Cys His Leu Met Val Ser Asn Pro Glu His His Leu Glu Asp Phe 65 70 75 80 Ala Arg Ala Gly Ala Asp Ile Ile Ser Ile His Val Glu Ala Thr Pro 85 90 95 His Ile His Gly Ala Leu Gln Lys Ile Arg Ser Leu Gly Val Lys Pro 100 105 110 Ser Val Val Ile Asn Pro Gly Thr Pro Val Glu Ala Ile Lys His Val 115 120 125 Leu His Leu Val Asp Gln Val Leu Val Met Thr Val Asn Pro Gly Phe 130 135 140 Gly Gly Gln Ala Phe Leu Pro Glu Thr Met Asp Lys Val Arg Glu Leu 145 150 155 160 Val Ala Leu Arg Glu Glu Lys Gly Leu Asn Phe Glu Ile Glu Val Asp 165 170 175 Gly Gly Ile Asp Asp Gln Thr Ile Ala Gln Ala Lys Glu Ala Gly Ala 180 185 190 Thr Val Phe Val Ala Gly Ser Tyr Val Phe Lys Gly Glu Val Asn Glu 195 200 205 Arg Val Gln Thr Leu Arg Lys Gln Leu Asp 210 215 <210> 217 <211> 558 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (558) <223> Streptococcus pneumoniae spr1804 gene <400> 217 atgaaagaga gaatttccca agttatcgtg gttgaaggtc gtgatgatac ggtcaatctc 60 aagcgttatt tcgatgtgga gacctatgag actcgaggtt ctgccatcaa tgaccaggac 120 atagagcgga ttcagcgtct acaccaacgt catggagtca ttgtctttac agacccagat 180 tttaatgggg agcggattcg gcgcatgatt atgacggcca ttccaacagt tcagcatgcc 240 tttctcaagc gagatgaagc tgttcccaag tccaagacca aggggcgttc tctgggaatt 300 gagcatgcca gctatgaaga cctgaaaacg gctctagctc aggtgacaga acaatttgaa 360 catgagagtc agtttgacat tagtcgtagc gatttgattc gccttggttt tctagcaggg 420 gcagacagcc gtaagcgtag agaatatctc ggagagactc tccgaatcgg ctattctaac 480 ggcaagcaac tcctcaaacg cctagagttg tttggggtta ctttggcaga agtggaagaa 540 gctatgaaat cttatgag 558 <210> 218 <211> 186 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (186) <223> Conserved hypothetical protein <400> 218 Met Lys Glu Arg Ile Ser Gln Val Ile Val Val Glu Gly Arg Asp Asp 1 5 10 15 Thr Val Asn Leu Lys Arg Tyr Phe Asp Val Glu Thr Tyr Glu Thr Arg 20 25 30 Gly Ser Ala Ile Asn Asp Gln Asp Ile Glu Arg Ile Gln Arg Leu His 35 40 45 Gln Arg His Gly Val Ile Val Phe Thr Asp Pro Asp Phe Asn Gly Glu 50 55 60 Arg Ile Arg Arg Met Ile Met Thr Ala Ile Pro Thr Val Gln His Ala 65 70 75 80 Phe Leu Lys Arg Asp Glu Ala Val Pro Lys Ser Lys Thr Lys Gly Arg 85 90 95 Ser Leu Gly Ile Glu His Ala Ser Tyr Glu Asp Leu Lys Thr Ala Leu 100 105 110 Ala Gln Val Thr Glu Gln Phe Glu His Glu Ser Gln Phe Asp Ile Ser 115 120 125 Arg Ser Asp Leu Ile Arg Leu Gly Phe Leu Ala Gly Ala Asp Ser Arg 130 135 140 Lys Arg Arg Glu Tyr Leu Gly Glu Thr Leu Arg Ile Gly Tyr Ser Asn 145 150 155 160 Gly Lys Gln Leu Leu Lys Arg Leu Glu Leu Phe Gly Val Thr Leu Ala 165 170 175 Glu Val Glu Glu Ala Met Lys Ser Tyr Glu 180 185 <210> 219 <211> 1077 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1077) <223> Streptococcus pneumoniae spr1825 gene <400> 219 gtgatttgta gtgattcaag ttactctttt cacaataaaa atttcatgat tttcataagg 60 aggaaatcac taatggtagt taaagttggt attaacggtt tcggacgtat cggtcgtctt 120 gctttccgtc gtatccaaaa cgtagaaggt gttgaagtta cacgcatcaa cgaccttaca 180 gatccagtta tgcttgcaca cttgttgaaa tacgacacaa ctcaaggtcg tttcgacggt 240 actgttgaag ttaaagaagg tggatttgaa gttaacggta aattcatcaa agtttctgct 300 gaacgtgatc cagaacaaat cgactgggct actgacggtg tagaaatcgt tcttgaagct 360 actggtttct ttgctaagaa agaagcagct gaaaaacacc ttaaaggtgg agctaaaaaa 420 gttgttatca ctgctcctgg tggaaacgac gttaaaacag ttgtattcaa cactaaccac 480 gacgttcttg acggtactga aacagttatc tcaggtgctt catgtactac aaactgcttg 540 gctccaatgg ctaaagctct tcaagacaac tttggtgttg ttgaaggatt gatgactact 600 atccacgctt acactggtga ccaaatgatc cttgacggac cacaccgtgg tggtgacctt 660 cgccgtgctc gcgctggtgc tgcaaacatc gttcctaact caactggtgc tgcaaaagct 720 atcggtcttg taatcccaga attgaatggt aaacttgatg gatctgcaca acgcgttcca 780 actccaactg gatcagttac tgaattggta gcagttcttg aaaagaacgt tactgttgat 840 gaagtgaacg cagctatgaa agcagcttca aacgaatcat acggttacac agaagatcca 900 atcgtatctt cagatatcgt aggtatgtct tacggttcat tgtttgacgc aactcaaact 960 aaagttcttg acgttgacgg taaacaattg gttaaagttg tatcatggta cgacaacgaa 1020 atgtcataca ctgcacaact tgttcgtact cttgaatact tcgcaaagat tgctaaa 1077 <210> 220 <211> 359 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (359) Glyceraldehyde 3-phosphate dehydrogenase <400> 220 Met Ile Cys Ser Asp Ser Ser Tyr Ser Phe His Asn Lys Asn Phe Met 1 5 10 15 Ile Phe Ile Arg Arg Lys Ser Leu Met Val Val Lys Val Gly Ile Asn 20 25 30 Gly Phe Gly Arg Ile Gly Arg Leu Ala Phe Arg Arg Ile Gln Asn Val 35 40 45 Glu Gly Val Glu Val Thr Arg Ile Asn Asp Leu Thr Asp Pro Val Met 50 55 60 Leu Ala His Leu Leu Lys Tyr Asp Thr Thr Gln Gly Arg Phe Asp Gly 65 70 75 80 Thr Val Glu Val Lys Glu Gly Gly Phe Glu Val Asn Gly Lys Phe Ile 85 90 95 Lys Val Ser Ala Glu Arg Asp Pro Glu Gln Ile Asp Trp Ala Thr Asp 100 105 110 Gly Val Glu Ile Val Leu Glu Ala Thr Gly Phe Phe Ala Lys Lys Glu 115 120 125 Ala Ala Glu Lys His Leu Lys Gly Gly Ala Lys Lys Val Val Ile Thr 130 135 140 Ala Pro Gly Gly Asn Asp Val Lys Thr Val Val Phe Asn Thr Asn His 145 150 155 160 Asp Val Leu Asp Gly Thr Glu Thr Val Ile Ser Gly Ala Ser Cys Thr 165 170 175 Thr Asn Cys Leu Ala Pro Met Ala Lys Ala Leu Gln Asp Asn Phe Gly 180 185 190 Val Val Glu Gly Leu Met Thr Thr Ile His Ala Tyr Thr Gly Asp Gln 195 200 205 Met Ile Leu Asp Gly Pro His Arg Gly Gly Asp Leu Arg Arg Ala Arg 210 215 220 Ala Gly Ala Ala Asn Ile Val Pro Asn Ser Thr Gly Ala Ala Lys Ala 225 230 235 240 Ile Gly Leu Val Ile Pro Glu Leu Asn Gly Lys Leu Asp Gly Ser Ala 245 250 255 Gln Arg Val Pro Thr Pro Thr Gly Ser Val Thr Glu Leu Val Ala Val 260 265 270 Leu Glu Lys Asn Val Thr Val Asp Glu Val Asn Ala Ala Met Lys Ala 275 280 285 Ala Ser Asn Glu Ser Tyr Gly Tyr Thr Glu Asp Pro Ile Val Ser Ser 290 295 300 Asp Ile Val Gly Met Ser Tyr Gly Ser Leu Phe Asp Ala Thr Gln Thr 305 310 315 320 Lys Val Leu Asp Val Asp Gly Lys Gln Leu Val Lys Val Val Ser Trp 325 330 335 Tyr Asp Asn Glu Met Ser Tyr Thr Ala Gln Leu Val Arg Thr Leu Glu 340 345 350 Tyr Phe Ala Lys Ile Ala Lys 355 <210> 221 <211> 369 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (369) <223> Streptococcus pneumoniae spr1853 gene <400> 221 ttgaagaaaa actttcgtgt aaaaagagag aaagatttta aggcgatttt caaggagggg 60 acaagttttg ctaatcgcaa atttgtggtc taccaattag aaaaccagaa aaaccatttt 120 cgagtaggtc tatcagttag caaaaaactg gggaatgccg tcactagaaa tcaaattaag 180 cgacggattc ggcatattat ccagaatgca aaagggagtc tggtagaaga tgtcgacttt 240 gttgtcattg ctcgaaaagg agtcgaaacc ttgggatacg cagagatgga gaaaaatcta 300 ctccatgtat taaaattatc aaagatttac cgggaaggaa atgggagtga aaaagaaact 360 aaagttgac 369 <210> 222 <211> 123 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (123) <223> Ribonuclease P-protein component <400> 222 Met Lys Lys Asn Phe Arg Val Lys Arg Glu Lys Asp Phe Lys Ala Ile 1 5 10 15 Phe Lys Glu Gly Thr Ser Phe Ala Asn Arg Lys Phe Val Val Tyr Gln 20 25 30 Leu Glu Asn Gln Lys Asn His Phe Arg Val Gly Leu Ser Val Ser Lys 35 40 45 Lys Leu Gly Asn Ala Val Thr Arg Asn Gln Ile Lys Arg Arg Ile Arg 50 55 60 His Ile Ile Gln Asn Ala Lys Gly Ser Leu Val Glu Asp Val Asp Phe 65 70 75 80 Val Val Ile Ala Arg Lys Gly Val Glu Thr Leu Gly Tyr Ala Glu Met 85 90 95 Glu Lys Asn Leu Leu His Val Leu Lys Leu Ser Lys Ile Tyr Arg Glu 100 105 110 Gly Asn Gly Ser Glu Lys Glu Thr Lys Val Asp 115 120 <210> 223 <211> 378 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (378) <223> Streptococcus pneumoniae spr1856 gene <400> 223 atgttggagc aagctcgttt gaaagtggag cagcaagcaa tcaagaatat ccagtttttg 60 gagcaagatt taccgaaaaa tcccttggag aaagagtttg attgccttgc tgttagtcgg 120 gttcttcatc atatgcctga tttggatgcg gctctctcac tgtttcatca acatttgaag 180 gaagatggga aactcatcat tgctgatttt accaagacag aagctaatca tcatggattt 240 gatttagctg aactggaaaa caagctaatt gagcatggtt tttcatctgt gcatagtcag 300 attctctata gtgctgaaga cctgtttcaa ggaaatcact cagaattctt tttaatagta 360 gcccaaaaat cactcgcc 378 <210> 224 <211> 126 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (126) <223> Conserved hypothetical protein <400> 224 Met Leu Glu Gln Ala Arg Leu Lys Val Glu Gln Gln Ala Ile Lys Asn 1 5 10 15 Ile Gln Phe Leu Glu Gln Asp Leu Pro Lys Asn Pro Leu Glu Lys Glu 20 25 30 Phe Asp Cys Leu Ala Val Ser Arg Val Leu His His Met Pro Asp Leu 35 40 45 Asp Ala Ala Leu Ser Leu Phe His Gln His Leu Lys Glu Asp Gly Lys 50 55 60 Leu Ile Ile Ala Asp Phe Thr Lys Thr Glu Ala Asn His His Gly Phe 65 70 75 80 Asp Leu Ala Glu Leu Glu Asn Lys Leu Ile Glu His Gly Phe Ser Ser 85 90 95 Val His Ser Gln Ile Leu Tyr Ser Ala Glu Asp Leu Phe Gln Gly Asn 100 105 110 His Ser Glu Phe Phe Leu Ile Val Ala Gln Lys Ser Leu Ala 115 120 125 <210> 225 <211> 1458 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1458) <223> Streptococcus pneumoniae spr1881 gene <400> 225 atgtcaaaag atatccgcgt acgttacgca ccaagtccaa caggactact acacatcgga 60 aatgctcgta cagcattgtt taattacttg tatgcgcgcc atcatggtgg aacatttctc 120 atccgtatcg aagatactga ccgtaaacgc catgtcgagg atggtgaacg ttcacaactt 180 gaaaaccttc gctggttagg catggattgg gatgaaagtc cagaatcaca tgagaattat 240 cgccagtctg agcgtttgga cttgtatcaa aaatacattg accaactatt agctgaagga 300 aaagcctata aatcttacgt tacagaagaa gagttggcag ctgaacgcga acgccaagaa 360 gtagctggcg aaacaccacg ctacatcaat gaataccttg gtatgagtga agaagaaaaa 420 gcagcttaca tcgcagaacg tgaagcagca gggatcatcc caactgttcg tttggctgtc 480 aatgagtcag gtatctacaa gtggcatgat atggtcaaag gcgatatcga atttgaaggt 540 ggcaatatcg gtggtgactg gattatccaa aagaaagacg gttacccaac ttacaacttt 600 gccgttgcca tcgatgacca tgatatgcaa atctctcatg ttatccgtgg agacgaccat 660 attgctaaca caccaaaaca gctcatggtt tatgaagcac ttggttggga agctccagag 720 tttggtcaca tgaccttgat tatcaactct gagactggta agaagttatc taaacgtgat 780 actaatacac ttcaatttat cgaagattac cgaaagaaag gttaccttcc agaagcagtc 840 tttaacttta ttgctcttct tggttggaac ccaggtggtg aagatgagat tttctctcgt 900 gaagaactca ttaaactttt tgatgaaaac cgcctcagca agtcaccagc agcctttgat 960 cagaaaaaac tagactggat gagcaatgat tatatcaaga atgcagacct tgaaaccatc 1020 tttgaaatgg caaaaccatt cttagaggaa gcaggccgtt tgactgacaa ggctgaaaaa 1080 ttagttgagc tctataaacc acaaatgaaa tcagtagatg agattatccc attgacagat 1140 cttttcttct cagatttccc agaattgaca gaagcagagc gcgaagtcat gacgggtgaa 1200 acagttccaa cagttcttga agcattcaaa gcaaaacttg aagcgatgac agatgataaa 1260 tttgtgacag aaaatatctt cccacaaatt aaagcagttc aaaaagaaac aggtattaaa 1320 gggaaaaatc ttttcatgcc tattcgtatc gcagtttcag gcgaaatgca tgggccagaa 1380 ttaccagata caattttctt gcttggacgt gaaaaatcaa ttcagcatat cgaaaacatg 1440 ctaaaagaaa tctctaaa 1458 <210> 226 <211> 486 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (486) <223> Glutamyl-tRNA synthetase <400> 226 Met Ser Lys Asp Ile Arg Val Arg Tyr Ala Pro Ser Pro Thr Gly Leu 1 5 10 15 Leu His Ile Gly Asn Ala Arg Thr Ala Leu Phe Asn Tyr Leu Tyr Ala 20 25 30 Arg His His Gly Gly Thr Phe Leu Ile Arg Ile Glu Asp Thr Asp Arg 35 40 45 Lys Arg His Val Glu Asp Gly Glu Arg Ser Gln Leu Glu Asn Leu Arg 50 55 60 Trp Leu Gly Met Asp Trp Asp Glu Ser Pro Glu Ser His Glu Asn Tyr 65 70 75 80 Arg Gln Ser Glu Arg Leu Asp Leu Tyr Gln Lys Tyr Ile Asp Gln Leu 85 90 95 Leu Ala Glu Gly Lys Ala Tyr Lys Ser Tyr Val Thr Glu Glu Glu Leu 100 105 110 Ala Ala Glu Arg Glu Arg Gln Glu Val Ala Gly Glu Thr Pro Arg Tyr 115 120 125 Ile Asn Glu Tyr Leu Gly Met Ser Glu Glu Glu Lys Ala Ala Tyr Ile 130 135 140 Ala Glu Arg Glu Ala Ala Gly Ile Ile Pro Thr Val Arg Leu Ala Val 145 150 155 160 Asn Glu Ser Gly Ile Tyr Lys Trp His Asp Met Val Lys Gly Asp Ile 165 170 175 Glu Phe Glu Gly Gly Asn Ile Gly Gly Asp Trp Ile Ile Gln Lys Lys 180 185 190 Asp Gly Tyr Pro Thr Tyr Asn Phe Ala Val Ala Ile Asp Asp His Asp 195 200 205 Met Gln Ile Ser His Val Ile Arg Gly Asp Asp His Ile Ala Asn Thr 210 215 220 Pro Lys Gln Leu Met Val Tyr Glu Ala Leu Gly Trp Glu Ala Pro Glu 225 230 235 240 Phe Gly His Met Thr Leu Ile Ile Asn Ser Glu Thr Gly Lys Lys Leu 245 250 255 Ser Lys Arg Asp Thr Asn Thr Leu Gln Phe Ile Glu Asp Tyr Arg Lys 260 265 270 Lys Gly Tyr Leu Pro Glu Ala Val Phe Asn Phe Ile Ala Leu Leu Gly 275 280 285 Trp Asn Pro Gly Gly Glu Asp Glu Ile Phe Ser Arg Glu Glu Leu Ile 290 295 300 Lys Leu Phe Asp Glu Asn Arg Leu Ser Lys Ser Pro Ala Ala Phe Asp 305 310 315 320 Gln Lys Lys Leu Asp Trp Met Ser Asn Asp Tyr Ile Lys Asn Ala Asp 325 330 335 Leu Glu Thr Ile Phe Glu Met Ala Lys Pro Phe Leu Glu Glu Ala Gly 340 345 350 Arg Leu Thr Asp Lys Ala Glu Lys Leu Val Glu Leu Tyr Lys Pro Gln 355 360 365 Met Lys Ser Val Asp Glu Ile Ile Pro Leu Thr Asp Leu Phe Phe Ser 370 375 380 Asp Phe Pro Glu Leu Thr Glu Ala Glu Arg Glu Val Met Thr Gly Glu 385 390 395 400 Thr Val Pro Thr Val Leu Glu Ala Phe Lys Ala Lys Leu Glu Ala Met 405 410 415 Thr Asp Asp Lys Phe Val Thr Glu Asn Ile Phe Pro Gln Ile Lys Ala 420 425 430 Val Gln Lys Glu Thr Gly Ile Lys Gly Lys Asn Leu Phe Met Pro Ile 435 440 445 Arg Ile Ala Val Ser Gly Glu Met His Gly Pro Glu Leu Pro Asp Thr 450 455 460 Ile Phe Leu Leu Gly Arg Glu Lys Ser Ile Gln His Ile Glu Asn Met 465 470 475 480 Leu Lys Glu Ile Ser Lys 485 <210> 227 <211> 1347 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1347) <223> Streptococcus pneumoniae spr1882 gene <400> 227 atgtcacata ttaaatttga ttattcaaaa gttttagaca aatttgttgc accacatgaa 60 gtggaataca tgcaatcaca agtaacagca gcagatgaat tgatccgtaa aggaactggt 120 gctggtagcg acttcttggg atggttggac cttcctgaaa aatacgaccg cgaagaattt 180 gaccgcatct tgaaagctgc tgagcaaatc aagtcagaca gcgatgtttt ggttgtgatc 240 ggtattggtg gatcttacct tggtgcaaaa gcagcaatcg acttcttgaa tcaccatttt 300 gctaacttgc aaacaaaaga agaacgcaaa gctccacaaa tcctttacgc aggaaactca 360 atctcatcta cttaccttgc tgacttggta gagtatgttg cagacaaaga cttctcagta 420 aacgtaattt ctaaatcagg tacaacaact gaaccagcga ttgctttccg tgtctttaaa 480 gaactcttgg ttaagaaata cggtcaagaa gaagctaaca aacgtatcta tgcaacaact 540 gaccgccaaa agggtgctgt taaggttgaa gcagacgcta acggttgggg aacatttgtt 600 gttccagatg atatcggtgg acgcttctca gtattgacag ccgttggttt gcttccaatc 660 gcagcatcag gagctgacat caaagctctt atggaaggtg cgaatgcagc tcgcaaagac 720 tacacttcag acaaaatctc tgaaaacgaa gcttaccaat acgcagctgt tcgtaacatc 780 ctttatcgta aaggctatgc aactgagatc ttggtaaact atgagccatc acttcaatac 840 ttctcagaat ggtggaaaca attggctggt gaatcagaag gaaaagacca aaaaggtatc 900 tacccaactt cagccaactt ctcaactgac ttgcactcac ttggtcaatt tatccaagaa 960 ggaactcgta tcatgtttga aacagttgtc cgtgttgaca aacctcgtaa aaacgtgctt 1020 attcctactt tggaagaaga ccttgacgga cttggttacc ttcaaggaaa agacgttgac 1080 tttgtaaaca aaaaagcaac tgacggtgtt cttcttgccc acacagatgg tgatgtacca 1140 aacatgtatg tgactcttcc agagcaagac gctttcactc ttggttacac tatctacttc 1200 ttcgaattgg caattgccct ttcaggttac ttgaatgcta tcaacccatt tgaccaacca 1260 ggtgttgaag cttataaacg taacatgttt gcccttcttg gaaaaccagg atttgaagaa 1320 ttgagcaaag aacttaacgc acgtcta 1347 <210> 228 <211> 449 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN <222> (1) .. (449) <223> Glucose-6-phosphate isomerase <400> 228 Met Ser His Ile Lys Phe Asp Tyr Ser Lys Val Leu Asp Lys Phe Val 1 5 10 15 Ala Pro His Glu Val Glu Tyr Met Gln Ser Gln Val Thr Ala Ala Asp 20 25 30 Glu Leu Ile Arg Lys Gly Thr Gly Ala Gly Ser Asp Phe Leu Gly Trp 35 40 45 Leu Asp Leu Pro Glu Lys Tyr Asp Arg Glu Glu Phe Asp Arg Ile Leu 50 55 60 Lys Ala Ala Glu Gln Ile Lys Ser Asp Ser Asp Val Leu Val Val Ile 65 70 75 80 Gly Ile Gly Gly Ser Tyr Leu Gly Ala Lys Ala Ala Ile Asp Phe Leu 85 90 95 Asn His His Phe Ala Asn Leu Gln Thr Lys Glu Glu Arg Lys Ala Pro 100 105 110 Gln Ile Leu Tyr Ala Gly Asn Ser Ile Ser Ser Thr Tyr Leu Ala Asp 115 120 125 Leu Val Glu Tyr Val Ala Asp Lys Asp Phe Ser Val Asn Val Ile Ser 130 135 140 Lys Ser Gly Thr Thr Thr Glu Pro Ala Ile Ala Phe Arg Val Phe Lys 145 150 155 160 Glu Leu Leu Val Lys Lys Tyr Gly Gln Glu Glu Ala Asn Lys Arg Ile 165 170 175 Tyr Ala Thr Thr Asp Arg Gln Lys Gly Ala Val Lys Val Glu Ala Asp 180 185 190 Ala Asn Gly Trp Gly Thr Phe Val Val Pro Asp Asp Ile Gly Gly Arg 195 200 205 Phe Ser Val Leu Thr Ala Val Gly Leu Leu Pro Ile Ala Ala Ser Gly 210 215 220 Ala Asp Ile Lys Ala Leu Met Glu Gly Ala Asn Ala Ala Arg Lys Asp 225 230 235 240 Tyr Thr Ser Asp Lys Ile Ser Glu Asn Glu Ala Tyr Gln Tyr Ala Ala 245 250 255 Val Arg Asn Ile Leu Tyr Arg Lys Gly Tyr Ala Thr Glu Ile Leu Val 260 265 270 Asn Tyr Glu Pro Ser Leu Gln Tyr Phe Ser Glu Trp Trp Lys Gln Leu 275 280 285 Ala Gly Glu Ser Glu Gly Lys Asp Gln Lys Gly Ile Tyr Pro Thr Ser 290 295 300 Ala Asn Phe Ser Thr Asp Leu His Ser Leu Gly Gln Phe Ile Gln Glu 305 310 315 320 Gly Thr Arg Ile Met Phe Glu Thr Val Val Arg Val Asp Lys Pro Arg 325 330 335 Lys Asn Val Leu Ile Pro Thr Leu Glu Glu Asp Leu Asp Gly Leu Gly 340 345 350 Tyr Leu Gln Gly Lys Asp Val Asp Phe Val Asn Lys Lys Ala Thr Asp 355 360 365 Gly Val Leu Leu Ala His Thr Asp Gly Asp Val Pro Asn Met Tyr Val 370 375 380 Thr Leu Pro Glu Gln Asp Ala Phe Thr Leu Gly Tyr Thr Ile Tyr Phe 385 390 395 400 Phe Glu Leu Ala Ile Ala Leu Ser Gly Tyr Leu Asn Ala Ile Asn Pro 405 410 415 Phe Asp Gln Pro Gly Val Glu Ala Tyr Lys Arg Asn Met Phe Ala Leu 420 425 430 Leu Gly Lys Pro Gly Phe Glu Glu Leu Ser Lys Glu Leu Asn Ala Arg 435 440 445 Leu <210> 229 <211> 1254 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1254) <223> Streptococcus pneumoniae spr1910 gene <400> 229 atgcacattt ttgatgagct aaaagagcgt ggtttgatat ttcaaacgac tgatgaagaa 60 gctttgcgta aagccctaga agaaggtcaa gtttcttatt atactggcta cgatccaact 120 gctgacagcc ttcacctagg ccaccttgtc gcaatcttga caagtcgtcg cttgcaacta 180 gcaggtcaca aaccttatgc gctcgttggc ggtgctacag gtctcatcgg agatccgtcc 240 ttcaaagatg ctgaacgtag tctccaaaca aaagacacag tagatggctg ggtcaagtct 300 atccaaggac aactttctcg ttttcttgac tttgaaaatg gcgaaaacaa ggctgtcatg 360 gtcaacaact acgactggtt tggcagcatc agcttcattg acttcctccg tgatattgga 420 aaatacttca cggtcaacta catgatgagt aaggaatctg ttaaaaaacg gatcgaaaca 480 ggaatttctt acactgagtt cgcttaccaa atcatgcaag ggtatgactt cttcgtcctt 540 aaccaagacc ataatgtcac tcttcaaatc ggtggttctg accagtgggg aaatatgaca 600 gctggtaccg aattgcttcg tcgtaaggcg gacaagactg gtcacgttat cactgttcca 660 ctaatcacag atgcaactgg taagaaattt ggtaaatcag aaggaaatgc cgtctggctc 720 aatcccgaaa agacttctcc atacgaaatg taccaattct ggatgaacgt gatggacgct 780 gacgctgttc gcttcttgaa aatctttact ttcttgtcac ttgatgagat tgaagatatt 840 cgtaaacaat ttgaagcagc gccacacgaa cgcttggctc aaaaagtctt ggctcgtgaa 900 gttgttacac ttgttcacgg agaagaagcc tacaaagaag cacttaacat cactgagcaa 960 ctctttgcag gaaacatcaa aaacctttct gtcaaagagc tcaaacaagg acttcgtggt 1020 gtgccaaact accaagtaca ggcagacgaa aacaacaata tcgtggaact gctcgtctca 1080 tctggtatag ttaactcaaa acgccaagcc cgtgaagacg tccaaaacgg agccatctac 1140 gtaaacggcg accgcatcca agagcttgac tatgtcttga gtgacgctga taagttagag 1200 aatgaactga ctgttatccg tcgtgggaag aaaaaatact ttgtattgac ttac 1254 <210> 230 <211> 418 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (418) <223> Tyrosyl-tRNA synthetase 1 <400> 230 Met His Ile Phe Asp Glu Leu Lys Glu Arg Gly Leu Ile Phe Gln Thr 1 5 10 15 Thr Asp Glu Glu Ala Leu Arg Lys Ala Leu Glu Glu Gly Gln Val Ser 20 25 30 Tyr Tyr Thr Gly Tyr Asp Pro Thr Ala Asp Ser Leu His Leu Gly His 35 40 45 Leu Val Ala Ile Leu Thr Ser Arg Arg Leu Gln Leu Ala Gly His Lys 50 55 60 Pro Tyr Ala Leu Val Gly Gly Ala Thr Gly Leu Ile Gly Asp Pro Ser 65 70 75 80 Phe Lys Asp Ala Glu Arg Ser Leu Gln Thr Lys Asp Thr Val Asp Gly 85 90 95 Trp Val Lys Ser Ile Gln Gly Gln Leu Ser Arg Phe Leu Asp Phe Glu 100 105 110 Asn Gly Glu Asn Lys Ala Val Met Val Asn Asn Tyr Asp Trp Phe Gly 115 120 125 Ser Ile Ser Phe Ile Asp Phe Leu Arg Asp Ile Gly Lys Tyr Phe Thr 130 135 140 Val Asn Tyr Met Met Ser Lys Glu Ser Val Lys Lys Arg Ile Glu Thr 145 150 155 160 Gly Ile Ser Tyr Thr Glu Phe Ala Tyr Gln Ile Met Gln Gly Tyr Asp 165 170 175 Phe Phe Val Leu Asn Gln Asp His Asn Val Thr Leu Gln Ile Gly Gly 180 185 190 Ser Asp Gln Trp Gly Asn Met Thr Ala Gly Thr Glu Leu Leu Arg Arg 195 200 205 Lys Ala Asp Lys Thr Gly His Val Ile Thr Val Pro Leu Ile Thr Asp 210 215 220 Ala Thr Gly Lys Lys Phe Gly Lys Ser Glu Gly Asn Ala Val Trp Leu 225 230 235 240 Asn Pro Glu Lys Thr Ser Pro Tyr Glu Met Tyr Gln Phe Trp Met Asn 245 250 255 Val Met Asp Ala Asp Ala Val Arg Phe Leu Lys Ile Phe Thr Phe Leu 260 265 270 Ser Leu Asp Glu Ile Glu Asp Ile Arg Lys Gln Phe Glu Ala Ala Pro 275 280 285 His Glu Arg Leu Ala Gln Lys Val Leu Ala Arg Glu Val Val Thr Leu 290 295 300 Val His Gly Glu Glu Ala Tyr Lys Glu Ala Leu Asn Ile Thr Glu Gln 305 310 315 320 Leu Phe Ala Gly Asn Ile Lys Asn Leu Ser Val Lys Glu Leu Lys Gln 325 330 335 Gly Leu Arg Gly Val Pro Asn Tyr Gln Val Gln Ala Asp Glu Asn Asn 340 345 350 Asn Ile Val Glu Leu Leu Val Ser Ser Gly Ile Val Asn Ser Lys Arg 355 360 365 Gln Ala Arg Glu Asp Val Gln Asn Gly Ala Ile Tyr Val Asn Gly Asp 370 375 380 Arg Ile Gln Glu Leu Asp Tyr Val Leu Ser Asp Ala Asp Lys Leu Glu 385 390 395 400 Asn Glu Leu Thr Val Ile Arg Arg Gly Lys Lys Lys Tyr Phe Val Leu 405 410 415 Thr Tyr <210> 231 <211> 204 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (204) <223> Streptococcus pneumoniae spr1914 gene <400> 231 atgttaaaaa aatatttttc aaaatataaa tggacggatt tattttggat tttatttgtt 60 attttgacct gcctctatat tggtaaccat gatttgttta ctctcaatca tcaagaattc 120 tcttttcgtg gtagcgtttg gggtctggta ctggccttat atcacttatt gttcattgat 180 aagtttgtaa tttcgaatcg aaaa 204 <210> 232 <211> 68 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (68) <223> Conserved hypothetical protein <400> 232 Met Leu Lys Lys Tyr Phe Ser Lys Tyr Lys Trp Thr Asp Leu Phe Trp 1 5 10 15 Ile Leu Phe Val Ile Leu Thr Cys Leu Tyr Ile Gly Asn His Asp Leu 20 25 30 Phe Thr Leu Asn His Gln Glu Phe Ser Phe Arg Gly Ser Val Trp Gly 35 40 45 Leu Val Leu Ala Leu Tyr His Leu Leu Phe Ile Asp Lys Phe Val Ile 50 55 60 Ser Asn Arg Lys 65 <210> 233 <211> 879 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (879) <223> Streptococcus pneumoniae spr1950 gene <400> 233 atggtaaaaa caatgaccat tgcaacgatt gatatcggag ggactgggat taagtttgcc 60 agtctgactc ctgatgggaa aatactggat aagacaagta tttcaacgcc tgaaaacttg 120 gaggatttac tagcgtggct agatcaacgc ttgtcagaac aggattacag tgggattgct 180 atgagtgttc caggtgcagt caatcaagag acaggtgtga ttgatggctt cagtgcggtg 240 ccctacatcc atggcttttc ttggtatgag gcgcttatct cttatcagct acctgtccat 300 ttagaaaatg atgccaactg cgttggactc agtgaactac tagctcatcc agagcttgaa 360 aatgcagcct gtgtcgtgat tgggacaggg attggcggag ccatgattat caatggtaga 420 cttcatcgag gttgccacgg tctgggtgga gaatttggct acatgacaac ccttgcccct 480 gctgaaaaac ttaataactg gtcgcaacta gcatcaactg ggaatatggt acgatacgtg 540 attgaaaaat ctggtcatac tgattgggac ggtcgcaaga tttaccaaga ggccgcagct 600 ggtaatgctc tttgtcaaga agccattgag cgcatgaacc gcaatctggc gcaaggcttg 660 ctcaatatcc agtatctgat cgatccagat gtcatcagtc tgggtggctc tatcagtcaa 720 aatccagatt ttatccaagg tgtcaagaag gctgttgaag actttgtcga tgcctacgaa 780 gaatacacgg tcgcaccagt tatccaggcc tgcacctatc acgcagatgc caatctctac 840 ggtgctcttg tcaactggct acaggaggaa aagcaatgg 879 <210> 234 <211> 293 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (293) <223> Conserved hypothetical protein <400> 234 Met Val Lys Thr Met Thr Ile Ala Thr Ile Asp Ile Gly Gly Thr Gly 1 5 10 15 Ile Lys Phe Ala Ser Leu Thr Pro Asp Gly Lys Ile Leu Asp Lys Thr 20 25 30 Ser Ile Ser Thr Pro Glu Asn Leu Glu Asp Leu Leu Ala Trp Leu Asp 35 40 45 Gln Arg Leu Ser Glu Gln Asp Tyr Ser Gly Ile Ala Met Ser Val Pro 50 55 60 Gly Ala Val Asn Gln Glu Thr Gly Val Ile Asp Gly Phe Ser Ala Val 65 70 75 80 Pro Tyr Ile His Gly Phe Ser Trp Tyr Glu Ala Leu Ile Ser Tyr Gln 85 90 95 Leu Pro Val His Leu Glu Asn Asp Ala Asn Cys Val Gly Leu Ser Glu 100 105 110 Leu Leu Ala His Pro Glu Leu Glu Asn Ala Ala Cys Val Val Ile Gly 115 120 125 Thr Gly Ile Gly Gly Ala Met Ile Ile Asn Gly Arg Leu His Arg Gly 130 135 140 Cys His Gly Leu Gly Gly Glu Phe Gly Tyr Met Thr Thr Leu Ala Pro 145 150 155 160 Ala Glu Lys Leu Asn Asn Trp Ser Gln Leu Ala Ser Thr Gly Asn Met 165 170 175 Val Arg Tyr Val Ile Glu Lys Ser Gly His Thr Asp Trp Asp Gly Arg 180 185 190 Lys Ile Tyr Gln Glu Ala Ala Ala Gly Asn Ala Leu Cys Gln Glu Ala 195 200 205 Ile Glu Arg Met Asn Arg Asn Leu Ala Gln Gly Leu Leu Asn Ile Gln 210 215 220 Tyr Leu Ile Asp Pro Asp Val Ile Ser Leu Gly Gly Ser Ile Ser Gln 225 230 235 240 Asn Pro Asp Phe Ile Gln Gly Val Lys Lys Ala Val Glu Asp Phe Val 245 250 255 Asp Ala Tyr Glu Glu Tyr Thr Val Ala Pro Val Ile Gln Ala Cys Thr 260 265 270 Tyr His Ala Asp Ala Asn Leu Tyr Gly Ala Leu Val Asn Trp Leu Gln 275 280 285 Glu Glu Lys Gln Trp 290 <210> 235 <211> 1149 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1149) <223> Streptococcus pneumoniae spr 1963 gene <400> 235 atggctacat tttatgttcc ggcagtcaac cttattggaa aaggtgttgt aaatgaagtg 60 ggtccttata tcaaggaact tggctataaa aaggcacttt tggtgacaga taagtacatc 120 gaaggcagtg atattttacc taagacttta aaaccactgg atacagaagg aatcgaatat 180 gtcatcttta gcgatgtaga gccaaaccct acttgtaaga atgtcacaga tggggtagct 240 gctttgcaag aacatggatg tgattttatc atcagtcttg gcgggggttc tccacaggat 300 gcagctagtt gtatttctat catggctaca aatggtggaa aaccacagga ttatgaaggg 360 cttcataagt ctgctaaaaa aggcttgcca gttgtggcta tcaatacaac ggcaggaact 420 tctgcagaaa tcactattaa ctatgtgatt actgatgaag aacgcaaggt taagatggta 480 atggttgaca agaatagcct tgccttgatt tctgttaatg acccggaact catgctttcc 540 aaacctaaag gcctgactgc tgctactggt atggatgctc tgactcacgc tgttgaagct 600 ttggtaacac ctggtgctta tgatgtaacc aagaaactgt ctattggtgc tattgagctt 660 atcaaggaat atcttcctcg tgctgtagca aacggacatg atattgaagc gcgtgaaggt 720 atggtcaatg ccatcttcct tggtggtatg agctttaata atgctggtct tggttatgtt 780 cactcaatgg ctcaccaact cggtgcagta tataatttgc cacatggtgt ttgctgtgcc 840 atgttgctac cagttataga acgtgaaaat gctaaacgtg taccagaagc tttccgcaat 900 gttgccaaag ccttgggact tcatgtagaa ggtaaatcag atcaagagtg tgccgattat 960 gcgattgctg agattgagaa gctttctgag acagtaggta ttcctaagaa attgactgaa 1020 cttggtattg aagaaaaaga tttcgacttt gaataccttt ctaagaatgc cttgattgat 1080 gcctgcgcac caggaaatcc atttatgcca accttagaag aaacgattgc cttttataaa 1140 gagttattt 1149 <210> 236 <211> 383 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (383) <223> Probable alcohol dehydrogenase <400> 236 Met Ala Thr Phe Tyr Val Pro Ala Val Asn Leu Ile Gly Lys Gly Val 1 5 10 15 Val Asn Glu Val Gly Pro Tyr Ile Lys Glu Leu Gly Tyr Lys Lys Ala 20 25 30 Leu Leu Val Thr Asp Lys Tyr Ile Glu Gly Ser Asp Ile Leu Pro Lys 35 40 45 Thr Leu Lys Pro Leu Asp Thr Glu Gly Ile Glu Tyr Val Ile Phe Ser 50 55 60 Asp Val Glu Pro Asn Pro Thr Cys Lys Asn Val Thr Asp Gly Val Ala 65 70 75 80 Ala Leu Gln Glu His Gly Cys Asp Phe Ile Ile Ser Leu Gly Gly Gly 85 90 95 Ser Pro Gln Asp Ala Ala Ser Cys Ile Ser Ile Met Ala Thr Asn Gly 100 105 110 Gly Lys Pro Gln Asp Tyr Glu Gly Leu His Lys Ser Ala Lys Lys Gly 115 120 125 Leu Pro Val Val Ala Ile Asn Thr Thr Ala Gly Thr Ser Ala Glu Ile 130 135 140 Thr Ile Asn Tyr Val Ile Thr Asp Glu Glu Arg Lys Val Lys Met Val 145 150 155 160 Met Val Asp Lys Asn Ser Leu Ala Leu Ile Ser Val Asn Asp Pro Glu 165 170 175 Leu Met Leu Ser Lys Pro Lys Gly Leu Thr Ala Ala Thr Gly Met Asp 180 185 190 Ala Leu Thr His Ala Val Glu Ala Leu Val Thr Pro Gly Ala Tyr Asp 195 200 205 Val Thr Lys Lys Leu Ser Ile Gly Ala Ile Glu Leu Ile Lys Glu Tyr 210 215 220 Leu Pro Arg Ala Val Ala Asn Gly His Asp Ile Glu Ala Arg Glu Gly 225 230 235 240 Met Ser Asn Ala Ile Phe Leu Gly Met Ser Phe Asn Asn Ala Gly 245 250 255 Leu Gly Tyr Val His Ser Met Ala His Gln Leu Gly Ala Val Tyr Asn 260 265 270 Leu Pro His Gly Val Cys Cys Ala Met Leu Leu Pro Val Ile Glu Arg 275 280 285 Glu Asn Ala Lys Arg Val Pro Glu Ala Phe Arg Asn Val Ala Lys Ala 290 295 300 Leu Gly Leu His Val Glu Gly Lys Ser Asp Gln Glu Cys Ala Asp Tyr 305 310 315 320 Ala Ile Ala Glu Ile Glu Lys Leu Ser Glu Thr Val Gly Ile Pro Lys 325 330 335 Lys Leu Thr Glu Leu Gly Ile Glu Glu Lys Asp Phe Asp Phe Glu Tyr 340 345 350 Leu Ser Lys Asn Ala Leu Ile Asp Ala Cys Ala Pro Gly Asn Pro Phe 355 360 365 Met Pro Thr Leu Glu Glu Thr Ile Ala Phe Tyr Lys Glu Leu Phe 370 375 380 <210> 237 <211> 438 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (438) <223> Streptococcus pneumoniae spr1971 gene <400> 237 atgttaaaac atataccgaa aaatatttct ccagatttat tgaagacttt aatggaaatg 60 ggacatggag atgaaatagt attagctgac gcgaattatc cttctgcctc atgtgcaaat 120 aagctaattc gttgtgatgg tgtaaatatt ccagaattat tagattccat tctgtattta 180 atgccattag atagttacgt cgatagttca attcagttta tgaacgttgt ttcgggtgat 240 gatattccta agatatgggg tacctataga cagatgattg aaggtcatgg tacagatctt 300 aaaacgatta cttatcttag aagagaagac ttttatgaac gtagtaagaa agcttatgct 360 attgttgcta caggagaaac ttcactttat gctaatatta tccttaagaa aggagtagtt 420 gttgaaagag aaaatgtt 438 <210> 238 <211> 146 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (146) <223> Fucose pathway protein, function unknown <400> 238 Met Leu Lys His Ile Pro Lys Asn Ile Ser Pro Asp Leu Leu Lys Thr 1 5 10 15 Leu Met Glu Met Gly His Gly Asp Glu Ile Val Leu Ala Asp Ala Asn 20 25 30 Tyr Pro Ser Ala Ser Cys Ala Asn Lys Leu Ile Arg Cys Asp Gly Val 35 40 45 Asn Ile Pro Glu Leu Leu Asp Ser Ile Leu Tyr Leu Met Pro Leu Asp 50 55 60 Ser Tyr Val Asp Ser Ser Ile Gln Phe Met Asn Val Val Ser Gly Asp 65 70 75 80 Asp Ile Pro Lys Ile Trp Gly Thr Tyr Arg Gln Met Ile Glu Gly His 85 90 95 Gly Thr Asp Leu Lys Thr Ile Thr Tyr Leu Arg Arg Glu Asp Phe Tyr 100 105 110 Glu Arg Ser Lys Lys Ala Tyr Ala Ile Val Ala Thr Gly Glu Thr Ser 115 120 125 Leu Tyr Ala Asn Ile Ile Leu Lys Lys Gly Val Val Val Glu Arg Glu 130 135 140 Asn val 145 <210> 239 <211> 1446 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1446) <223> Streptococcus pneumoniae spr1973 gene <400> 239 gtggggatta taataaagtt aatcaaggac gaagagagaa gaaaaatgga agcggtttta 60 gcaatagatt taggtgcgac ttctggaaga gcaatcgttg gttacctttc tgaaaataaa 120 ctagtaatgg aagaaataaa tcgcttttct aatctaccta ttagagtaaa agggcattta 180 tcttgggata ttgactttct actagctaaa attcttgaaa gtatccgctt ggctaatact 240 agttacaaga ttttatctat cggtattgac acatggggag ttgattttgg actgattgat 300 aatgaaggta agctgttatt acaacctgtt cattatcgtg atgaaagaac aaagggagtg 360 ttaaaggaaa tatctgaaat gactgaatta gaaaaactgt attcagagac aggaaatcag 420 attatggaga taaatacctt gtttcaactc tttaaggcac gtcaagaatc tcctgactct 480 ttctataaga ccaataagat tcttttaatg ccagatttgt ttaattatct cttgacaggt 540 aagtttgcta cagaaaaaag cattgcttca acaactcaat tatttgatcc taggagtcaa 600 aattggaatc agaatatctt aaaactattt gaattggatt catctttact tcctgaaatt 660 gtttcagagg gaaatgttct tggaaggata aaagaggagt atggtttagg cgatattcct 720 gttgtgaatg tttgtagtca tgatacagca agcgcgattg tctcagtacc taagacagaa 780 ggtagtttat ttatttcatc aggtacttgg tctttggttg gagtggaact tacttcaccg 840 attcttacta ccgaatcctt cagttatgga tttacaaatg aagtcggtaa agatggagtg 900 attacatttc tgaagaattg tacagggttg tggatcatag aggaactaag acgttcattt 960 gaacgaagag ggaaagccta ttcttttgat gatattagga caatggtgga gaaagaaaaa 1020 gaaaatcttc ctctgattga tactgaatca actgaatttg caacagaatc tgatatgcac 1080 aagactttga cagaatatct agcttatcat catgaaacta gagagtggac agatggacaa 1140 ctatttaaga ttgtttatga aagcctagct gaaacgtata ggaaagcgat agagttacta 1200 gaagaactaa ctcataaggt ttataagagg atatatgtga ttggaggagg tgctagagcc 1260 agttacttta accaaatgat tgctgataga actggtaaag aggttcttac aggttcgact 1320 gagggtacag ctgtggggaa tattgttgtg cagctcatag ctatgggaca attaaaaggg 1380 atggaagagg ctcaccatgt tattgaggag tttctacaat tagagagtta ttactcccaa 1440 aagaat 1446 <210> 240 <211> 482 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (482) <223> Fucose kinase <400> 240 Met Gly Ile Ile Ile Lys Leu Ile Lys Asp Glu Glu Arg Arg Lys Met 1 5 10 15 Glu Ala Val Leu Ala Ile Asp Leu Gly Ala Thr Ser Gly Arg Ala Ile 20 25 30 Val Gly Tyr Leu Ser Glu Asn Lys Leu Val Met Glu Glu Ile Asn Arg 35 40 45 Phe Ser Asn Leu Pro Ile Arg Val Lys Gly His Leu Ser Trp Asp Ile 50 55 60 Asp Phe Leu Leu Ala Lys Ile Leu Glu Ser Ile Arg Leu Ala Asn Thr 65 70 75 80 Ser Tyr Lys Ile Leu Ser Ile Gly Ile Asp Thr Trp Gly Val Asp Phe 85 90 95 Gly Leu Ile Asp Asn Glu Gly Lys Leu Leu Leu Gln Pro Val His Tyr 100 105 110 Arg Asp Glu Arg Thr Lys Gly Val Leu Lys Glu Ile Ser Glu Met Thr 115 120 125 Glu Leu Glu Lys Leu Tyr Ser Glu Thr Gly Asn Gln Ile Met Glu Ile 130 135 140 Asn Thr Leu Phe Gln Leu Phe Lys Ala Arg Gln Glu Ser Pro Asp Ser 145 150 155 160 Phe Tyr Lys Thr Asn Lys Ile Leu Leu Met Pro Asp Leu Phe Asn Tyr 165 170 175 Leu Leu Thr Gly Lys Phe Ala Thr Glu Lys Ser Ile Ala Ser Thr Thr 180 185 190 Gln Leu Phe Asp Pro Arg Ser Gln Asn Trp Asn Gln Asn Ile Leu Lys 195 200 205 Leu Phe Glu Leu Asp Ser Ser Leu Leu Pro Glu Ile Val Ser Glu Gly 210 215 220 Asn Val Leu Gly Arg Ile Lys Glu Glu Tyr Gly Leu Gly Asp Ile Pro 225 230 235 240 Val Val Asn Val Cys Ser His Asp Thr Ala Ser Ala Ile Val Ser Val 245 250 255 Pro Lys Thr Glu Gly Ser Leu Phe Ile Ser Ser Gly Thr Trp Ser Leu 260 265 270 Val Gly Val Glu Leu Thr Ser Pro Ile Leu Thr Thr Glu Ser Phe Ser 275 280 285 Tyr Gly Phe Thr Asn Glu Val Gly Lys Asp Gly Val Ile Thr Phe Leu 290 295 300 Lys Asn Cys Thr Gly Leu Trp Ile Ile Glu Glu Leu Arg Arg Ser Phe 305 310 315 320 Glu Arg Arg Gly Lys Ala Tyr Ser Phe Asp Asp Ile Arg Thr Met Val 325 330 335 Glu Lys Glu Lys Glu Asn Leu Pro Leu Ile Asp Thr Glu Ser Thr Glu 340 345 350 Phe Ala Thr Glu Ser Asp Met His Lys Thr Leu Thr Glu Tyr Leu Ala 355 360 365 Tyr His His Glu Thr Arg Glu Trp Thr Asp Gly Gln Leu Phe Lys Ile 370 375 380 Val Tyr Glu Ser Leu Ala Glu Thr Tyr Arg Lys Ala Ile Glu Leu Leu 385 390 395 400 Glu Glu Leu Thr His Lys Val Tyr Lys Arg Ile Tyr Val Ile Gly Gly 405 410 415 Gly Ala Arg Ala Ser Tyr Phe Asn Gln Met Ile Ala Asp Arg Thr Gly 420 425 430 Lys Glu Val Leu Thr Gly Ser Thr Glu Gly Thr Ala Val Gly Asn Ile 435 440 445 Val Val Gln Leu Ile Ala Met Gly Gln Leu Lys Gly Met Glu Glu Ala 450 455 460 His His Val Ile Glu Glu Phe Leu Gln Leu Glu Ser Tyr Tyr Ser Gln 465 470 475 480 Lys asn <210> 241 <211> 201 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (201) <223> Streptococcus pneumoniae spr1987 gene <400> 241 gtgccgaaat ggtcaaatcc tgattatgtc aacgaattag acccaaaaat cgttgatatg 60 ctagtagaat ttcacaagtc acaaggcact ttggaaactc ccgaggcgca agcagaaatc 120 gcccaaaaac gtgaagaaat cgagcaaagg agagctgagc ttgagggtaa aaaacaagag 180 cttttgaacc gcttgaacaa a 201 <210> 242 <211> 67 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (67) <223> Hypothetical protein, truncation <400> 242 Met Pro Lys Trp Ser Asn Pro Asp Tyr Val Asn Glu Leu Asp Pro Lys 1 5 10 15 Ile Val Asp Met Leu Val Glu Phe His Lys Ser Gln Gly Thr Leu Glu 20 25 30 Thr Pro Glu Ala Gln Ala Glu Ile Ala Gln Lys Arg Glu Glu Ile Glu 35 40 45 Gln Arg Arg Ala Glu Leu Glu Gly Lys Lys Gln Glu Leu Leu Asn Arg 50 55 60 Leu Asn Lys 65 <210> 243 <211> 660 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (660) <223> Streptococcus pneumoniae spr2012 gene <400> 243 atgaagtgct tgttatgtgg gcagactatg aagactgttt taacttttag tagtctctta 60 cttctgagga atgatgactc ttgtctttgt tcagactgtg attctacttt tgaaagaatt 120 ggggaagaga actgtccaaa ttgtatgaaa acagagttgt caacaaagtg tcaagattgt 180 caactttggt gtaaagaagg agttggagtc agtcatagag cgatttttac ttacaatcaa 240 gctatgaagg attttttcag tcggtataag tttgatggag acttcctgtt aagaaaagtt 300 ttcgcttcat ttttaagtga ggagttgaaa aagtacaaag agtatcaatt tgttgtaatt 360 cccctaagtc ctgatagata tgctaataga ggatttaatc aggttgaggg cttggtagag 420 gcagcaggct ttgagtatct agatttatta gagaaaagag aagagagagc cagttcttct 480 aaaaatcgtt cagagcgctt ggggacagaa cttcctttct ttattaaaag tggagtcact 540 attcctaaaa aaatcctact tatagatgat atctatacta caggagcaac tataaatcgt 600 gttaagaaac tgttggaaga agccggtgct aaggatgtaa aaacattttc ccttgtaaga 660 660 <210> 244 <211> 220 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (220) <223> competence factor ComFC <400> 244 Met Lys Cys Leu Leu Cys Gly Gln Thr Met Lys Thr Val Leu Thr Phe 1 5 10 15 Ser Ser Leu Leu Leu Leu Arg Asn Asp Asp Ser Cys Leu Cys Ser Asp 20 25 30 Cys Asp Ser Thr Phe Glu Arg Ile Gly Glu Glu Asn Cys Pro Asn Cys 35 40 45 Met Lys Thr Glu Leu Ser Thr Lys Cys Gln Asp Cys Gln Leu Trp Cys 50 55 60 Lys Glu Gly Val Gly Val Ser His Arg Ala Ile Phe Thr Tyr Asn Gln 65 70 75 80 Ala Met Lys Asp Phe Phe Ser Arg Tyr Lys Phe Asp Gly Asp Phe Leu 85 90 95 Leu Arg Lys Val Phe Ala Ser Phe Leu Ser Glu Glu Leu Lys Lys Tyr 100 105 110 Lys Glu Tyr Gln Phe Val Val Ile Pro Leu Ser Pro Asp Arg Tyr Ala 115 120 125 Asn Arg Gly Phe Asn Gln Val Glu Gly Leu Val Glu Ala Ala Gly Phe 130 135 140 Glu Tyr Leu Asp Leu Leu Glu Lys Arg Glu Glu Arg Ala Ser Ser Ser 145 150 155 160 Lys Asn Arg Ser Glu Arg Leu Gly Thr Glu Leu Pro Phe Phe Ile Lys 165 170 175 Ser Gly Val Thr Ile Pro Lys Lys Ile Leu Leu Ile Asp Asp Ile Tyr 180 185 190 Thr Thr Gly Ala Thr Ile Asn Arg Val Lys Lys Leu Leu Glu Glu Ala 195 200 205 Gly Ala Lys Asp Val Lys Thr Phe Ser Leu Val Arg 210 215 220 <210> 245 <211> 1296 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1296) <223> Streptococcus pneumoniae spr 2013 gene <400> 245 atgaaagtaa atttagatta tctcggtcgt ttatttactg agaatgaatt aacagaagaa 60 gaacgtcagt tggcggagaa acttccagca atgagaaagg agaaggggaa acttttctgt 120 caacgttgta atagtactat tctagaagaa tggtatttgc ccatcggtgc ttactattgt 180 cgagagtgct tgctgatgaa gcgagtcaga agtgatcaaa ctttatacta ttttccgcag 240 gaggattttc cgaagcaaga tgttctcaaa tggcgcggcc aattaactcc ttttcaagag 300 aaggtgtcag agggactgct tcaagcagta gacaagcaaa agccaacctt agttcatgcg 360 gtaacaggag ctggaaagac agaaatgatt tatcaagtag tggctaaagt gatcaatgcg 420 ggtggtgcag tgtgtttggc tagtcctcgc atagatgttt gtttggagct gtacaagcgc 480 ctgcaacagg atttttcttg cggaatagct ttgctacatg gagaatcgga accttatttt 540 cgaacaccac tagttgttgc aacaacccat cagttattga agttttatca agcttttgat 600 ttgctgatag tggatgaagt agatgctttt ccttatgttg ataatcccat gctttaccac 660 gctgtcaaga atagtgtaaa ggagaatgga ttgagaatct ttttaacagc gacttcgacc 720 aatgagttag ataaaaaggt ccgtttagga gaactaaaaa gactgaattt accgagacgg 780 tttcatggaa atccgttgat tattccaaaa ccaatttggt tatcggattt taatcgctac 840 ttagacaaga atcgtttgtc accaaagtta aagtcctata ttgagaagca gagaaagaca 900 gcttatccgt tactcatttt tgcttcagaa attaagaaag gggagcagtt agcagaaatc 960 ttacaggagc aatttccaaa tgagaaaatt ggctttgtat cttctgtaac agaggatcga 1020 ttagagcaag tacaagcttt tcgagatgga gaactgacaa tacttatcag tacgacaatc 1080 ttggagcgtg gagttacctt cccttgtgtg gatgttttcg tagtagaggc caatcatcgt 1140 ttgtttacca agtctagttt gattcagatt ggtggacgag ttggacgaag catggataga 1200 ccgacaggag atttgctttt cttccatgat gggttaaatg cttcaatcaa gaaggcgatt 1260 aaggaaattc agatgatgaa taaggaggct ggtcta 1296 <210> 246 <211> 432 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (432) <223> competence factor ComFA <400> 246 Met Lys Val Asn Leu Asp Tyr Leu Gly Arg Leu Phe Thr Glu Asn Glu 1 5 10 15 Leu Thr Glu Glu Glu Arg Gln Leu Ala Glu Lys Leu Pro Ala Met Arg 20 25 30 Lys Glu Lys Gly Lys Leu Phe Cys Gln Arg Cys Asn Ser Thr Ile Leu 35 40 45 Glu Glu Trp Tyr Leu Pro Ile Gly Ala Tyr Tyr Cys Arg Glu Cys Leu 50 55 60 Leu Met Lys Arg Val Arg Ser Asp Gln Thr Leu Tyr Tyr Phe Pro Gln 65 70 75 80 Glu Asp Phe Pro Lys Gln Asp Val Leu Lys Trp Arg Gly Gln Leu Thr 85 90 95 Pro Phe Gln Glu Lys Val Ser Glu Gly Leu Leu Gln Ala Val Asp Lys 100 105 110 Gln Lys Pro Thr Leu Val His Ala Val Thr Gly Ala Gly Lys Thr Glu 115 120 125 Met Ile Tyr Gln Val Val Ala Lys Val Ile Asn Ala Gly Gly Ala Val 130 135 140 Cys Leu Ala Ser Pro Arg Ile Asp Val Cys Leu Glu Leu Tyr Lys Arg 145 150 155 160 Leu Gln Gln Asp Phe Ser Cys Gly Ile Ala Leu Leu His Gly Glu Ser 165 170 175 Glu Pro Tyr Phe Arg Thr Pro Leu Val Val Ala Thr Thr His Gln Leu 180 185 190 Leu Lys Phe Tyr Gln Ala Phe Asp Leu Leu Ile Val Asp Glu Val Asp 195 200 205 Ala Phe Pro Tyr Val Asp Asn Pro Met Leu Tyr His Ala Val Lys Asn 210 215 220 Ser Val Lys Glu Asn Gly Leu Arg Ile Phe Leu Thr Ala Thr Ser Thr 225 230 235 240 Asn Glu Leu Asp Lys Lys Val Arg Leu Gly Glu Leu Lys Arg Leu Asn 245 250 255 Leu Pro Arg Arg Phe His Gly Asn Pro Leu Ile Ile Pro Lys Pro Ile 260 265 270 Trp Leu Ser Asp Phe Asn Arg Tyr Leu Asp Lys Asn Arg Leu Ser Pro 275 280 285 Lys Leu Lys Ser Tyr Ile Glu Lys Gln Arg Lys Thr Ala Tyr Pro Leu 290 295 300 Leu Ile Phe Ala Ser Glu Ile Lys Lys Gly Glu Gln Leu Ala Glu Ile 305 310 315 320 Leu Gln Glu Gln Phe Pro Asn Glu Lys Ile Gly Phe Val Ser Ser Val 325 330 335 Thr Glu Asp Arg Leu Glu Gln Val Gln Ala Phe Arg Asp Gly Glu Leu 340 345 350 Thr Ile Leu Ile Ser Thr Thr Ile Leu Glu Arg Gly Val Thr Phe Pro 355 360 365 Cys Val Asp Val Phe Val Val Glu Ala Asn His Arg Leu Phe Thr Lys 370 375 380 Ser Ser Leu Ile Gln Ile Gly Gly Arg Val Gly Arg Ser Met Asp Arg 385 390 395 400 Pro Thr Gly Asp Leu Leu Phe Phe His Asp Gly Leu Asn Ala Ser Ile 405 410 415 Lys Lys Ala Ile Lys Glu Ile Gln Met Met Asn Lys Glu Ala Gly Leu 420 425 430 <210> 247 <211> 543 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (543) <223> Streptococcus pneumoniae spr2027 gene <400> 247 atgaaaaaag aacaaattcc caatctctta acaataggtc gaattctctt tatacctatt 60 tttatcttta ttttaacgat aggaaattcg atagagagtc atatagttgc agctattatc 120 tttgctgttg ccagtattac cgactattta gatggatatt tagctcgtaa atggaatgtg 180 gtcagtaatt ttggtaaatt tgcagatcct atggcggata agttactagt tatgtcggct 240 tttattatgt tgattgagtt aggtatggct ccggcttgga ttgttgcagt gattatctgt 300 cgtgagttag ctgtgacagg tttaaggctt ttattggttg aaactggtgg aacaatttta 360 gcagcagcaa tgcctggaaa aattaaaact tttagtcaga tgtttgctat tattttcttg 420 ctattacatt ggactttgct tggtcaagtt ctactttatg tagccttatt tttcactatc 480 tactctggct atgactattt caagggtagt gcctatgtat ttaaagggac atttggttcg 540 aaa 543 <210> 248 <211> 181 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (181) <223> Phosphotidylglycerophosphate synthase <400> 248 Met Lys Lys Glu Gln Ile Pro Asn Leu Leu Thr Ile Gly Arg Ile Leu 1 5 10 15 Phe Ile Pro Ile Phe Ile Phe Ile Leu Thr Ile Gly Asn Ser Ile Glu 20 25 30 Ser His Ile Val Ala Ala Ile Ile Phe Ala Val Ala Ser Ile Thr Asp 35 40 45 Tyr Leu Asp Gly Tyr Leu Ala Arg Lys Trp Asn Val Val Ser Asn Phe 50 55 60 Gly Lys Phe Ala Asp Pro Met Ala Asp Lys Leu Leu Val Met Ser Ala 65 70 75 80 Phe Ile Met Leu Ile Glu Leu Gly Met Ala Pro Ala Trp Ile Val Ala 85 90 95 Val Ile Ile Cys Arg Glu Leu Ala Val Thr Gly Leu Arg Leu Leu Leu 100 105 110 Val Glu Thr Gly Gly Thr Ile Leu Ala Ala Ala Met Pro Gly Lys Ile 115 120 125 Lys Thr Phe Ser Gln Met Phe Ala Ile Ile Phe Leu Leu Leu His Trp 130 135 140 Thr Leu Leu Gly Gln Val Leu Leu Tyr Val Ala Leu Phe Phe Thr Ile 145 150 155 160 Tyr Ser Gly Tyr Asp Tyr Phe Lys Gly Ser Ala Tyr Val Phe Lys Gly 165 170 175 Thr Phe Gly Ser Lys 180 <210> 249 <211> 1023 <212> DNA <213> Streptococcus pneumoniae <220> <221> gene (222) (1) .. (1023) <223> Streptococcus pneumoniae spr2034 gene <400> 249 atgactaaac ccattatttt aacaggagac cgtccaacag gaaaattgca tattggacat 60 tatgttggaa gtctcaaaaa tcgagtatta ttacaggaag aggataagta tgatatgttt 120 gtgttcttgg ctgaccaaca agccttgaca gatcatgcca aagatcctca aaccattgta 180 gagtctatcg gaaatgtggc tttggattat cttgcagttg gattggatcc aaataagtca 240 actattttta ttcaaagcca gattccagag ttggctgagt tgtctatgta ttatatgaat 300 ctagtttcgt tagcacgttt ggagcgaaat ccaacagtca agacagagat ttctcagaaa 360 ggatttggag aaagcattcc gacaggattc ttggtctatc caatcgctca agcagctgat 420 atcacagctt tcaaggctaa ttatgttcct gttgggacag atcagaaacc aatgattgag 480 caaactcgtg aaattgttcg ttcttttaac aatgcatata actgtgatgt cttggtagag 540 ccggaaggta tttatccaga aaatgagaga gcagggcgtt tgcctggttt agatggaaat 600 gctaaaatgt ctaaatcact aaataatggt atttatttag ctgatgatgc ggatactttg 660 cgtaaaaaag taatgagtat gtatacagat ccagatcata tccgcgttga ggatccaggt 720 aagattgagg gaaatatggt tttccattat ctagatgttt ttggtcgtcc agaagatgct 780 caagaaattg ctgatatgaa agaacgttat caacgaggtg gtcttggtga tgtgaagacc 840 aagcgttatc tacttgaaat attagaacgt gaactgggtc ctattcgtga gcgccgtatt 900 gaatttgcta aggatatggg agaagtttat aatatgattc aaaaaggtag tgaaagagcg 960 cgtgaagttg cgggtcaaac cctatctgag gtaaaagggg caatgggact tcattacttt 1020 aac 1023 <210> 250 <211> 341 <212> PRT <213> Streptococcus pneumoniae <220> <221> CHAIN (222) (1) .. (341) <223> Tryptophanyl-tRNA synthetase <400> 250 Met Thr Lys Pro Ile Ile Leu Thr Gly Asp Arg Pro Thr Gly Lys Leu 1 5 10 15 His Ile Gly His Tyr Val Gly Ser Leu Lys Asn Arg Val Leu Leu Gln 20 25 30 Glu Glu Asp Lys Tyr Asp Met Phe Val Phe Leu Ala Asp Gln Gln Ala 35 40 45 Leu Thr Asp His Ala Lys Asp Pro Gln Thr Ile Val Glu Ser Ile Gly 50 55 60 Asn Val Ala Leu Asp Tyr Leu Ala Val Gly Leu Asp Pro Asn Lys Ser 65 70 75 80 Thr Ile Phe Ile Gln Ser Gln Ile Pro Glu Leu Ala Glu Leu Ser Met 85 90 95 Tyr Tyr Met Asn Leu Val Ser Leu Ala Arg Leu Glu Arg Asn Pro Thr 100 105 110 Val Lys Thr Glu Ile Ser Gln Lys Gly Phe Gly Glu Ser Ile Pro Thr 115 120 125 Gly Phe Leu Val Tyr Pro Ile Ala Gln Ala Ala Asp Ile Thr Ala Phe 130 135 140 Lys Ala Asn Tyr Val Pro Val Gly Thr Asp Gln Lys Pro Met Ile Glu 145 150 155 160 Gln Thr Arg Glu Ile Val Arg Ser Phe Asn Asn Ala Tyr Asn Cys Asp 165 170 175 Val Leu Val Glu Pro Glu Gly Ile Tyr Pro Glu Asn Glu Arg Ala Gly 180 185 190 Arg Leu Pro Gly Leu Asp Gly Asn Ala Lys Met Ser Lys Ser Leu Asn 195 200 205 Asn Gly Ile Tyr Leu Ala Asp Asp Ala Asp Thr Leu Arg Lys Lys Val 210 215 220 Met Ser Met Tyr Thr Asp Pro Asp His Ile Arg Val Glu Asp Pro Gly 225 230 235 240 Lys Ile Glu Gly Asn Met Val Phe His Tyr Leu Asp Val Phe Gly Arg 245 250 255 Pro Glu Asp Ala Gln Glu Ile Ala Asp Met Lys Glu Arg Tyr Gln Arg 260 265 270 Gly Gly Leu Gly Asp Val Lys Thr Lys Arg Tyr Leu Leu Glu Ile Leu 275 280 285 Glu Arg Glu Leu Gly Pro Ile Arg Glu Arg Arg Ile Glu Phe Ala Lys 290 295 300 Asp Met Gly Glu Val Tyr Asn Met Ile Gln Lys Gly Ser Glu Arg Ala 305 310 315 320 Arg Glu Val Ala Gly Gln Thr Leu Ser Glu Val Lys Gly Ala Met Gly 325 330 335 Leu His Tyr Phe Asn 340 <210> 251 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying KanR <400> 251 aacagtgaat tggagttcgt cttgtt 26 <210> 252 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0603 gene <400> 252 gctttttaga catctaaatc taggta 26 <210> 253 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0003 gene <400> 253 cgaacaatat ccacgaatcc aag 23 <210> 254 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0003 gene <400> 254 gacgaactcc aattcactgt taacttgata catactactc tcct 44 <210> 255 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0003 gene <400> 255 agatttagat gtctaaaaag ctgactgaga acccttagtt agag 44 <210> 256 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0003 gene <400> 256 gctgcacgcg tcaacttatc tac 23 <210> 257 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0047 gene <400> 257 tgcggatgaa ccagatggtt cag 23 <210> 258 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0047 gene <400> 258 gacgaactcc aattcactgt tgtcaactta gcagcatctg gatg 44 <210> 259 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0047 gene <400> 259 agatttagat gtctaaaaag cgagattgtg atcattgatg acga 44 <210> 260 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0047 gene <400> 260 catgacacct gcacgctccg tac 23 <210> 261 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0081 gene <400> 261 gcagatgata gtttgacaga gca 23 <210> 262 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0081 gene <400> 262 gacgaactcc aattcactgt tgtcatgtgg tagtctttga aggc 44 <210> 263 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0081 gene <400> 263 agatttagat gtctaaaaag ccgaagcagc gacagtggac ggag 44 <210> 264 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0081 gene <400> 264 gtcaccatga tataacctgg tac 23 <210> 265 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0129 gene <400> 265 cgatgacaaa ccaagttctt gtg 23 <210> 266 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0129 gene <400> 266 gacgaactcc aattcactgt tgcgagagaa agagccttgc tgga 44 <210> 267 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0129 gene <400> 267 agatttagat gtctaaaaag ctaccgagtc tggtgagtcc taca 44 <210> 268 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0129 gene <400> 268 cacactctga gctgccatga ggt 23 <210> 269 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0131 gene <400> 269 taccgagtct ggtgagtcct aca 23 <210> 270 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0131 gene <400> 270 gacgaactcc aattcactgt tcacactctg agctgccatg aggt 44 <210> 271 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0131 gene <400> 271 agatttagat gtctaaaaag cgtctcagag aacgcctagc aact 44 <210> 272 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0131 gene <400> 272 tctgagccga gctatgaacc gag 23 <210> 273 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0139 gene <400> 273 aagacaggtt agcttgtgaa cgt 23 <210> 274 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0139 gene <400> 274 gacgaactcc aattcactgt tctcatcata atctgtcgga agcg 44 <210> 275 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0139 gene <400> 275 agatttagat gtctaaaaag cactgaagca gaagcaatta agct 44 <210> 276 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0139 gene <400> 276 gaccgagcct tgtacaatac aga 23 <210> 277 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0145 gene <400> 277 actatcaaga aggactctct gta 23 <210> 278 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0145 gene <400> 278 gacgaactcc aattcactgt tctacggcaa ggtgaagctg acgt 44 <210> 279 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0145 gene <400> 279 agatttagat gtctaaaaag ccgcaatcta gtcggaacag ttct 44 <210> 280 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0145 gene <400> 280 cgtacggttg ttggtcgctt gga 23 <210> 281 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0156 gene <400> 281 gaattgtgag tcccttgcct agt 23 <210> 282 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0156 gene <400> 282 gacgaactcc aattcactgt tttgtcttca tatgtacctc catg 44 <210> 283 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0156 gene <400> 283 agatttagat gtctaaaaag ccatgtcgca gctattatcg caga 44 <210> 284 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0156 gene <400> 284 agcactgatt cagtatccta cag 23 <210> 285 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0173 gene <400> 285 tggacttcgg ctgtctctat gac 23 <210> 286 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0173 gene <400> 286 gacgaactcc aattcactgt tcagattgag aacttcattg acac 44 <210> 287 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0173 gene <400> 287 agatttagat gtctaaaaag ctgcgtgcag ctctataaac atca 44 <210> 288 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0173 gene <400> 288 tctccactca atacgtaacc tac 23 <210> 289 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0210 gene <400> 289 gtagccgtat cacttcatct atc 23 <210> 290 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0210 gene <400> 290 gacgaactcc aattcactgt tgtgcaacat ggaattgttc tacg 44 <210> 291 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0210 gene <400> 291 agatttagat gtctaaaaag ccgtgaagat gataagcctg agac 44 <210> 292 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0210 gene <400> 292 ctgatgtaag atcacgtaca cga 23 <210> 293 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0243 gene <400> 293 tcagagatgt tgataccaat cag 23 <210> 294 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0243 gene <400> 294 gacgaactcc aattcactgt tgagcatggc tgataacttg agca 44 <210> 295 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0243 gene <400> 295 agatttagat gtctaaaaag ctgtcggtgc taacgaagat ggct 44 <210> 296 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0243 gene <400> 296 cttaccgtca ccagctacac gtc 23 <210> 297 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0266 gene <400> 297 tgttgaatcg tgcaggctgg att 23 <210> 298 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0266 gene <400> 298 gacgaactcc aattcactgt tgctggagtg cctgctcaag agca 44 <210> 299 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0266 gene <400> 299 agatttagat gtctaaaaag ctacagagga agagttagct gact 44 <210> 300 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0266 gene <400> 300 catgatggcc acatctactt gct 23 <210> 301 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0267 gene <400> 301 tgctcttgag caggcactcc agc 23 <210> 302 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0267 gene <400> 302 gacgaactcc aattcactgt tgttgatccg tccggtagtt agca 44 <210> 303 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0267 gene <400> 303 agatttagat gtctaaaaag cagcaagtag atgtggccat catg 44 <210> 304 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0267 gene <400> 304 agcgtcctct cctacagcct cga 23 <210> 305 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0268 gene <400> 305 agcaagtaga tgtggccatc atg 23 <210> 306 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0268 gene <400> 306 gacgaactcc aattcactgt tagcgtcctc tcctacagcc tcga 44 <210> 307 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0268 gene <400> 307 agatttagat gtctaaaaag ccactgcaac cttgacgaca gtag 44 <210> 308 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0268 gene <400> 308 gcgttccgct atgtaaggat gag 23 <210> 309 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0269 gene <400> 309 tcgaggctgt aggagaggac gct 23 <210> 310 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0269 gene <400> 310 gacgaactcc aattcactgt tacgttccac cagtttatag gcta 44 <210> 311 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0269 gene <400> 311 agatttagat gtctaaaaag cctcatcctt acatagcgga acgc 44 <210> 312 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0269 gene <400> 312 cgtcaactac gtaccattta cgt 23 <210> 313 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0304 gene <400> 313 tgagacaacg actgagttag cag 23 <210> 314 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0304 gene <400> 314 gacgaactcc aattcactgt ttcatatctt actccgctat tcta 44 <210> 315 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0304 gene <400> 315 agatttagat gtctaaaaag cagatgttcg tgctaacaca gcta 44 <210> 316 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0304 gene <400> 316 cattcctggt ggagagccat aga 23 <210> 317 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0338 gene <400> 317 tggccttcag gagaagtggt act 23 <210> 318 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0338 gene <400> 318 gacgaactcc aattcactgt ttcttactat gtgcctgacc gaca 44 <210> 319 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0338 gene <400> 319 agatttagat gtctaaaaag ctcgtgaagc catccaagtg gttc 44 <210> 320 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0338 gene <400> 320 tctagcacca acataatcat agc 23 <210> 321 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0356 gene <400> 321 aggatggcat tctccgctct cag 23 <210> 322 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0356 gene <400> 322 gacgaactcc aattcactgt ttgctccaat attgggcata acca 44 <210> 323 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0356 gene <400> 323 agatttagat gtctaaaaag cagtaacaac accagaatat gaca 44 <210> 324 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0356 gene <400> 324 tgtgcagtct gcttagagta agt 23 <210> 325 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0359 gene <400> 325 atgtgagtga cagagattac cta 23 <210> 326 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0359 gene <400> 326 gacgaactcc aattcactgt tcacgaccga tattaccggc acca 44 <210> 327 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0359 gene <400> 327 agatttagat gtctaaaaag ctgctgcagt agacaggatt gttc 44 <210> 328 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0359 gene <400> 328 gataagactg acggagtcag ctca 24 <210> 329 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0372 gene <400> 329 acgacaggca cttcctacct ctc 23 <210> 330 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0372 gene <400> 330 gacgaactcc aattcactgt ttccacattg tcgtcttcgt cagc 44 <210> 331 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0372 gene <400> 331 agatttagat gtctaaaaag cgtcgtgata cgcgtggctt gatc 44 <210> 332 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0372 gene <400> 332 gttcgagtag gaggaaccag cct 23 <210> 333 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0393 gene <400> 333 tcttgctcaa cgctattgcc agc 23 <210> 334 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0393 gene <400> 334 gacgaactcc aattcactgt tgagctctac gtggacttca agtc 44 <210> 335 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0393 gene <400> 335 agatttagat gtctaaaaag cacgaagctg ctgttgccga cttc 44 <210> 336 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0393 gene <400> 336 tcaattccca tagaatgacc tct 23 <210> 337 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0395 gene <400> 337 acgttggtta tagaggtcat aca 23 <210> 338 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0395 gene <400> 338 gacgaactcc aattcactgt tgattggcaa cgtgtgttac ctct 44 <210> 339 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0395 gene <400> 339 agatttagat gtctaaaaag cgagttgcac aatctccttg tctc 44 <210> 340 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0395 gene <400> 340 gctgacttct tcgacgatag cac 23 <210> 341 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0478 gene <400> 341 caagacggat aaccgtggct tgt 23 <210> 342 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0478 gene <400> 342 gacgaactcc aattcactgt ttgtctgact gaccatagcg tctg 44 <210> 343 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0478 gene <400> 343 agatttagat gtctaaaaag ccatgagcgt ggctcgtgaa gcag 44 <210> 344 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0478 gene <400> 344 caattcttca cccgatatga tgc 23 <210> 345 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0530 gene <400> 345 atgagagaga atatcggtcg tta 23 <210> 346 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0530 gene <400> 346 gacgaactcc aattcactgt tagcttctgc tgcgcgcaag atag 44 <210> 347 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0530 gene <400> 347 agatttagat gtctaaaaag cgtatcgact tcttggcagc tggt 44 <210> 348 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0530 gene <400> 348 actccatcta atcgacagta cga 23 <210> 349 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0585 gene <400> 349 cttgactcgt cgctatgccg atg 23 <210> 350 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0585 gene <400> 350 gacgaactcc aattcactgt tacggcacca tagcgctcac caat 44 <210> 351 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0585 gene <400> 351 agatttagat gtctaaaaag cacatcaacg ctatgcagta tgtg 44 <352> 352 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0585 gene <400> 352 tggctagctt gccacggctg tga 23 <210> 353 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0604 gene <400> 353 atagcatcag ataaggacac gct 23 <210> 354 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0604 gene <400> 354 gacgaactcc aattcactgt taccatcttc gatgaacttg ggca 44 <210> 355 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0604 gene <400> 355 agatttagat gtctaaaaag catcgcagcg cgtgtgtcag gagt 44 <210> 356 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0604 gene <400> 356 agctagagcc acctcttctt cag 23 <210> 357 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0626 gene <400> 357 caatggcgca acaagttatt cct 23 <210> 358 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0626 gene <400> 358 gacgaactcc aattcactgt tctccaccgg cttcattatg aaga 44 <210> 359 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0626 gene <400> 359 agatttagat gtctaaaaag cagacatgac tttggaagaa gcta 44 <210> 360 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0626 gene <400> 360 gacaccagaa catcctctaa tag 23 <210> 361 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0646 gene <400> 361 atgtagacct actcctattg gag 23 <210> 362 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0646 gene <400> 362 gacgaactcc aattcactgt ttgtatccat agcaccgagt ccat 44 <210> 363 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0646 gene <400> 363 agatttagat gtctaaaaag ctgtaagcat cgagtagagc acat 44 <210> 364 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0646 gene <400> 364 gagtggaacg tagatcatct gct 23 <210> 365 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0666 gene <400> 365 tgatgaactg gtagcgcagt tag 23 <210> 366 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0666 gene <400> 366 gacgaactcc aattcactgt tcgtagagca gtcgttccgt tgtc 44 <210> 367 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0666 gene <400> 367 agatttagat gtctaaaaag cacagcggat tgcgattgcg cgtg 44 <210> 368 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0666 gene <400> 368 ttagctccga ccaagcgcat gat 23 <210> 369 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0670 gene <400> 369 tgccagtgtt catctctctt ggt 23 <210> 370 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0670 gene <400> 370 gacgaactcc aattcactgt tcacacgaac ttcgacatct gagt 44 <210> 371 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0670 gene <400> 371 agatttagat gtctaaaaag caccagttga ctcagttgat gcag 44 <210> 372 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0670 gene <400> 372 ctcagataca gattcagacg tga 23 <210> 373 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0671 gene <400> 373 accagttgac tcagttgatg cag 23 <210> 374 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0671 gene <400> 374 gacgaactcc aattcactgt tctcagatac agattcagac gtga 44 <210> 375 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0671 gene <400> 375 agatttagat gtctaaaaag catgctgatc aagatccact ggac 44 <210> 376 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0671 gene <400> 376 agaacagtat cccagtatag tta 23 <210> 377 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0729 gene <400> 377 gagaagttgt cgttatcatc ggt 23 <210> 378 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0729 gene <400> 378 gacgaactcc aattcactgt ttcaataatc tgtgtcatat cact 44 <210> 379 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0729 gene <400> 379 agatttagat gtctaaaaag ctggtctgga catccagtca tgat 44 <210> 380 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0729 gene <400> 380 gtaccaccaa gcagcagcag acg 23 <210> 381 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0755 gene <400> 381 gctgattcat taaggcgctc tga 23 <210> 382 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0755 gene <400> 382 gacgaactcc aattcactgt tacttgtcca atccagagac caga 44 <210> 383 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0755 gene <400> 383 agatttagat gtctaaaaag ctgtctagtg tcacagcatc gatc 44 <210> 384 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0755 gene <400> 384 gaacagtgct acacagatgt gac 23 <210> 385 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0756 gene <400> 385 acttgtccaa tccagagacc aga 23 <210> 386 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0756 gene <400> 386 gacgaactcc aattcactgt tgctgattca ttaaggcgct ctga 44 <210> 387 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0756 gene <400> 387 agatttagat gtctaaaaag cctatcaagg cccgtgatgc tcgt 44 <210> 388 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0756 gene <400> 388 gacgcggcgt tcggcgcgcg cta 23 <210> 389 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0788 gene <400> 389 atcgcctgac cagcgcatct tca 23 <210> 390 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0788 gene <400> 390 gacgaactcc aattcactgt ttcatcgttg tagtaatcac cgac 44 <210> 391 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0788 gene <400> 391 agatttagat gtctaaaaag ctgaccgtta ccgtcaggca gaga 44 <210> 392 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0788 gene <400> 392 agtgattgat atcgtcttct gtc 23 <210> 393 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0796 gene <400> 393 ggccttctta caggcagatg ata 23 <210> 394 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0796 gene <400> 394 gacgaactcc aattcactgt tacaactaca ccttcaattc cgtg 44 <210> 395 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0796 gene <400> 395 agatttagat gtctaaaaag ccttagctga aggtgtgatg tcag 44 <210> 396 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0796 gene <400> 396 aacgcaatca cttcacgagt tga 23 <210> 397 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0797 gene <400> 397 cttagctgaa ggtgtgatgt cag 23 <210> 398 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0797 gene <400> 398 gacgaactcc aattcactgt taacgcaatc acttcacgag ttga 44 <210> 399 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0797 gene <400> 399 agatttagat gtctaaaaag cggtatcgaa gtaccgttcg aaat 44 <210> 400 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0797 gene <400> 400 ggttgtagat agaactgacg aag 23 <210> 401 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0845 gene <400> 401 tggtcttgag tatccggacg act 23 <210> 402 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0845 gene <400> 402 gacgaactcc aattcactgt tcggcaaggg cttcacctga taac 44 <210> 403 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0845 gene <400> 403 agatttagat gtctaaaaag ctctttggcg tggagaacct gcag 44 <210> 404 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0845 gene <400> 404 aacacgcgct tctggaatcg taa 23 <210> 405 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0856 gene <400> 405 caagctctaa tggagggaaa cgt 23 <210> 406 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0856 gene <400> 406 gacgaactcc aattcactgt taatcctcct acaagcaagc ccag 44 <210> 407 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0856 gene <400> 407 agatttagat gtctaaaaag ccagtagatg tcaaaggtgc tgtc 44 <210> 408 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0856 gene <400> 408 tgaccttaac agtgtcaggc aga 23 <210> 409 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0861 gene <400> 409 gtcttctctt ggagttggag tgg 23 <210> 410 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0861 gene <400> 410 gacgaactcc aattcactgt tgcggactcg taaatataca agcc 44 <210> 411 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0861 gene <400> 411 agatttagat gtctaaaaag cacagctagg catcaagcca ctca 44 <210> 412 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0861 gene <400> 412 gacgtttgcg tgatacaacg cca 23 <210> 413 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0881 gene <400> 413 agtggaagct gccacagatg atg 23 <210> 414 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0881 gene <400> 414 gacgaactcc aattcactgt tcaggtgtat gcttgcttct caag 44 <210> 415 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0881 gene <400> 415 agatttagat gtctaaaaag ctctgaggac aaggacatct gtcg 44 <210> 416 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0881 gene <400> 416 cgtgatcctt gccttgcaag agc 23 <210> 417 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0882 gene <400> 417 tctgaggaca aggacatctg tcg 23 <210> 418 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0882 gene <400> 418 gacgaactcc aattcactgt tcgtgatcct tgccttgcaa gagc 44 <210> 419 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0882 gene <400> 419 agatttagat gtctaaaaag ccgtgccttc agcgagcgtt ggat 44 <210> 420 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0882 gene <400> 420 gacgctgacg catcaagtct aca 23 <210> 421 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0906 gene <400> 421 acgtgacggc ttagtgcctg ctg 23 <210> 422 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0906 gene <400> 422 gacgaactcc aattcactgt ttctgcatca tagatggctg cgat 44 <210> 423 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0906 gene <400> 423 agatttagat gtctaaaaag cctctcctga acaagaacca agtc 44 <210> 424 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0906 gene <400> 424 tgcagcaaca gcattatctg ctc 23 <210> 425 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0965 gene <400> 425 gtaggcaatc agctacaatc gct 23 <210> 426 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0965 gene <400> 426 gacgaactcc aattcactgt tctaccgatg accgtatcct tctg 44 <210> 427 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0965 gene <400> 427 agatttagat gtctaaaaag ctggtgcaat gaggcagtaa gaat 44 <210> 428 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0965 gene <400> 428 tccgacaatt ccatatctca cct 23 <210> 429 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0972 gene <400> 429 atcgctaata ttcagcacgc tat 23 <210> 430 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0972 gene <400> 430 gacgaactcc aattcactgt ttctcaaacg tctgtacatc tagc 44 <210> 431 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0972 gene <400> 431 agatttagat gtctaaaaag ccgatgtcag tacagactac ctat 44 <210> 432 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0972 gene <400> 432 agttgcttca atccattgac ctc 23 <210> 433 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0978 gene <400> 433 cagagaagcg cgctaagtat ctg 23 <210> 434 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0978 gene <400> 434 gacgaactcc aattcactgt tactgcttgt cctctacaac gctg 44 <210> 435 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0978 gene <400> 435 agatttagat gtctaaaaag ctccagaacg aaactatctc tatc 44 <210> 436 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0978 gene <400> 436 cgactctcat taacaatctg ctc 23 <210> 437 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0979 gene <400> 437 gagcagattg ttaatgagag tcg 23 <210> 438 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0979 gene <400> 438 gacgaactcc aattcactgt ttcattgacc ttagcagaag ttga 44 <210> 439 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0979 gene <400> 439 agatttagat gtctaaaaag ctcgtcaggc tatcactcgt gcta 44 <210> 440 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0979 gene <400> 440 tctggaacct ctatcatagc atc 23 <210> 441 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0982 gene <400> 441 cgtctggctg aggaattgcc tca 23 <210> 442 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0982 gene <400> 442 gacgaactcc aattcactgt ttctgagtat ggataatatc tagc 44 <210> 443 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0982 gene <400> 443 agatttagat gtctaaaaag ctagtgagat tgtccgtgac ttgc 44 <210> 444 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0982 gene <400> 444 gatagtcgga tccatatcca gat 23 <210> 445 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0992 gene <400> 445 taccgtcgcg accatgatca ata 23 <210> 446 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0992 gene <400> 446 gacgaactcc aattcactgt taccagcctt gtccatagct tcga 44 <210> 447 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0992 gene <400> 447 agatttagat gtctaaaaag ctgttgctct cttaacgatg aagt 44 <210> 448 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0992 gene <400> 448 tagtatatag gaggagagcc ctg 23 <210> 449 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0996 gene <400> 449 acatcagaag tgaccttgat gac 23 <210> 450 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0996 gene <400> 450 gacgaactcc aattcactgt tactcttggt tactaagcgc ttca 44 <210> 451 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0996 gene <400> 451 agatttagat gtctaaaaag cacaagagca cctggtggca ctct 44 <210> 452 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0996 gene <400> 452 tgatagcgat gattatcacc ttc 23 <210> 453 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1001 gene <400> 453 ggattgcttg gttcagatgt tgt 23 <210> 454 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1001 gene <400> 454 gacgaactcc aattcactgt tgacagtagc caatgatggt agta 44 <210> 455 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1001 gene <400> 455 agatttagat gtctaaaaag ccatgttgat gcagttcagg ctat 44 <210> 456 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1001 gene <400> 456 gagttggaag ccttcaccgc tgt 23 <210> 457 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1024 gene <400> 457 gtagatatca aggatgggtt aca 23 <210> 458 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1024 gene <400> 458 gacgaactcc aattcactgt tactcagtcg catagcgatt gagc 44 <210> 459 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1024 gene <400> 459 agatttagat gtctaaaaag ctgcggcagc aggaactctg cgtc 44 <210> 460 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1024 gene <400> 460 gcaacgcgga ttgatacaac gta 23 <210> 461 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1036 gene <400> 461 tgctgtgtca atatctacat atc 23 <210> 462 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1036 gene <400> 462 gacgaactcc aattcactgt tgattggagc gtcagagtga gaac 44 <210> 463 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1036 gene <400> 463 agatttagat gtctaaaaag cgctgctgtt cgtacatctg caga 44 <210> 464 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1036 gene <400> 464 tcagacacac cactattagc atc 23 <210> 465 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1063 gene <400> 465 agtaagtcac gtcttcaaga tgt 23 <210> 466 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1063 gene <400> 466 gacgaactcc aattcactgt ttgctggacg tgcgtgaata cctg 44 <210> 467 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1063 gene <400> 467 agatttagat gtctaaaaag cgctgacgta actatctcag ctga 44 <210> 468 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1063 gene <400> 468 caagtgtacg tgccatgata gct 23 <210> 469 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1064 gene <400> 469 tcagctgaga tagttacgtc agc 23 <210> 470 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1064 gene <400> 470 gacgaactcc aattcactgt tacgtactca ggttgctcat caag 44 <210> 471 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1064 gene <400> 471 agatttagat gtctaaaaag ccttgatgag caacctgagt acgt 44 <210> 472 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1064 gene <400> 472 gaagagctga gttgatagaa cgt 23 <210> 473 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1065 gene <400> 473 tgctggacgt gcgtgaatac ctg 23 <210> 474 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1065 gene <400> 474 gacgaactcc aattcactgt tagtaagtca cgtcttcaag atgt 44 <210> 475 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1065 gene <400> 475 agatttagat gtctaaaaag cacgttctat caactcagct cttc 44 <210> 476 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1065 gene <400> 476 gcacgaacca tagcacggat aga 23 <210> 477 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1081 gene <400> 477 cacaagattg tcgctcgtac tga 23 <210> 478 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1081 gene <400> 478 gacgaactcc aattcactgt tgctagatta tgaacaatat ctgt 44 <210> 479 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1081 gene <400> 479 agatttagat gtctaaaaag cacattcttg ctcagttgat ggac 44 <210> 480 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1081 gene <400> 480 gtgtcctcag aactgagagg agt 23 <210> 481 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1111 gene <400> 481 agctatcgat agcgtccgct cta 23 <210> 482 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1111 gene <400> 482 gacgaactcc aattcactgt ttgagtcatt aagacagtga cttg 44 <210> 483 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1111 gene <400> 483 agatttagat gtctaaaaag catggtaacc agtacagctc tagc 44 <210> 484 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1111 gene <400> 484 tgtagtcgtg gaacaatggc tag 23 <210> 485 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1150 gene <400> 485 atattgtcct gaatcatgcg aag 23 <210> 486 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1150 gene <400> 486 gacgaactcc aattcactgt tactgagttg aagtaaccat agtc 44 <210> 487 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1150 gene <400> 487 agatttagat gtctaaaaag cacttgtgat gagtttatcg acgc 44 <210> 488 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1150 gene <400> 488 agagagagta tcgaacagta tag 23 <210> 489 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1158 gene <400> 489 agctctactt tacctagtag agc 23 <210> 490 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1158 gene <400> 490 gacgaactcc aattcactgt ttgattacgt gcacgcaggt catc 44 <210> 491 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1158 gene <400> 491 agatttagat gtctaaaaag cctggtcaag gatttatctc agga 44 <210> 492 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1158 gene <400> 492 atcgcgctcc ttgcctgcct cacg 24 <210> 493 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1282 gene <400> 493 gtggtttctt atgatgatgc ggt 23 <210> 494 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1282 gene <400> 494 gacgaactcc aattcactgt ttccaacggc atgactgaga acat 44 <210> 495 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1282 gene <400> 495 agatttagat gtctaaaaag ctgagtacaa gtttgtcgct gaag 44 <210> 496 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1282 gene <400> 496 catcaacgta ctcttaccaa ctc 23 <210> 497 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1312 gene <400> 497 tgaccaagaa gcgactaaga ttg 23 <210> 498 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1312 gene <400> 498 gacgaactcc aattcactgt tcagcataca aggccgcagt catc 44 <210> 499 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1312 gene <400> 499 agatttagat gtctaaaaag ctactgtgct gtgtgtgatg gtgc 44 <210> 500 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1312 gene <400> 500 gatatgaacg gtactccttg cca 23 <210> 501 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1329 gene <400> 501 actcttatat ctggttcgaa gag 23 <210> 502 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1329 gene <400> 502 gacgaactcc aattcactgt ttgatggaga aggcttcatg acca 44 <210> 503 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1329 gene <400> 503 agatttagat gtctaaaaag cacgcagaat agtgagtaat actg 44 <210> 504 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1329 gene <400> 504 ctcttgctca tccaagagaa cag 23 <210> 505 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1359 gene <400> 505 tgaatacttc cgtgatgtgg aag 23 <210> 506 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1359 gene <400> 506 gacgaactcc aattcactgt tagctggcat ggtgatcata gacg 44 <210> 507 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1359 gene <400> 507 agatttagat gtctaaaaag ctcgatatca gtcgtgcaga acgt 44 <210> 508 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1359 gene <400> 508 gaccactatc tagtgacctg cga 23 <210> 509 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1360 gene <400> 509 acgtggcttg tcagaccaac cta 23 <210> 510 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1360 gene <400> 510 gacgaactcc aattcactgt tacagagatt ggacgacctg tgtc 44 <210> 511 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1360 gene <400> 511 agatttagat gtctaaaaag ctgaatactt ccgtgatgtg gaag 44 <210> 512 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1360 gene <400> 512 agctggcatg gtgatcatag acg 23 <210> 513 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1361 gene <400> 513 agcttatcgt gaactgtcgc tct 23 <210> 514 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1361 gene <400> 514 gacgaactcc aattcactgt tacgacctag cttagcagcc gata 44 <210> 515 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1361 gene <400> 515 agatttagat gtctaaaaag cacgtggctt gtcagaccaa ccta 44 <210> 516 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1361 gene <400> 516 acagagattg gacgacctgt gtc 23 <210> 517 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1362 gene <400> 517 agtggcagta gacgagtcgg ata 23 <210> 518 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1362 gene <400> 518 gacgaactcc aattcactgt tagctggtgc ttctactgga cgag 44 <210> 519 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1362 gene <400> 519 agatttagat gtctaaaaag cagcttatcg tgaactgtcg ctct 44 <210> 520 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1362 gene <400> 520 acgacctagc ttagcagccg ata 23 <210> 521 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1364 gene <400> 521 atcagtagca cgccaaccag ata 23 <210> 522 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1364 gene <400> 522 gacgaactcc aattcactgt tactgttacg tgcatcagtt actc 44 <210> 523 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1364 gene <400> 523 agatttagat gtctaaaaag ctgatcagta tatcgatcag ctag 44 <210> 524 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1364 gene <400> 524 acggatatct gtaaagtcac cta 23 <210> 525 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1409 gene <400> 525 tatcggtatg ggaatcactg gta 23 <210> 526 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1409 gene <400> 526 gacgaactcc aattcactgt ttagcttcat cctgccatac ggtc 44 <210> 527 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1409 gene <400> 527 agatttagat gtctaaaaag ctattgcagg caatgactat acga 44 <210> 528 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1409 gene <400> 528 agatatacca ggtcgttgcg ata 23 <210> 529 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1410 gene <400> 529 accgacatag aacatgatga tag 23 <210> 530 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1410 gene <400> 530 gacgaactcc aattcactgt ttgatgataa ccacagctag gatg 44 <210> 531 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1410 gene <400> 531 agatttagat gtctaaaaag ctatcggtat gggaatcact ggta 44 <210> 532 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1410 gene <400> 532 tagcttcatc ctgccatacg gtc 23 <210> 533 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1411 gene <400> 533 tgatgataac cacagctagg atg 23 <210> 534 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1411 gene <400> 534 gacgaactcc aattcactgt taccgacata gaacatgatg atag 44 <210> 535 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1411 gene <400> 535 agatttagat gtctaaaaag ctacactaga gatgaatcct gact 44 <210> 536 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1411 gene <400> 536 agtggtcaag gttcgagaag aca 23 <210> 537 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1413 gene <400> 537 tgctccagta gttggaacga ctg 23 <210> 538 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1413 gene <400> 538 gacgaactcc aattcactgt tagtcaacga tttctcctgt ctca 44 <210> 539 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1413 gene <400> 539 agatttagat gtctaaaaag cgagaattga gcaagcgaga ttgt 44 <540> 540 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1413 gene <540> 540 taggagtaga taagtcttga atg 23 <210> 541 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1419 gene <400> 541 cttaccagcg tattgctgaa tgt 23 <210> 542 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1419 gene <400> 542 gacgaactcc aattcactgt taccagtccg aatctctgga gaga 44 <210> 543 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1419 gene <400> 543 agatttagat gtctaaaaag ctatcagaag tctcagatgc cttg 44 <210> 544 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1419 gene <400> 544 tctatccaat gtctcttggt cac 23 <210> 545 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1427 gene <400> 545 tgtaagctat tccaaaccag tct 23 <210> 546 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1427 gene <400> 546 gacgaactcc aattcactgt tctgagctaa ctccacgcat tcat 44 <210> 547 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1427 gene <400> 547 agatttagat gtctaaaaag cgagaatgtg tctatcacac gtga 44 <210> 548 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1427 gene <400> 548 gattcttacg gttcaacata gtg 23 <210> 549 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1429 gene <400> 549 agtagtatac taaggtagta atc 23 <210> 550 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1429 gene <400> 550 gacgaactcc aattcactgt tatcaagata gcatgattca gagt 44 <210> 551 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1429 gene <400> 551 agatttagat gtctaaaaag cagtgattgt cactcacatt catg 44 <210> 552 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1429 gene <400> 552 gagctgtcgc atccgcaata gca 23 <210> 553 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1430 gene <400> 553 tagctatcgt agtttattac aga 23 <210> 554 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1430 gene <400> 554 gacgaactcc aattcactgt ttgcatatac cagtgcactt ggtg 44 <210> 555 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1430 gene <400> 555 agatttagat gtctaaaaag catctaccgt tacttgtcag aact 44 <210> 556 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1430 gene <400> 556 attctatact gtcattctat cta 23 <210> 557 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1432 gene <400> 557 atgtcgccaa gccagattgc tga 23 <210> 558 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1432 gene <400> 558 gacgaactcc aattcactgt tatcaagagc tggagcagcg atac 44 <210> 559 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1432 gene <400> 559 agatttagat gtctaaaaag catcagcttc acaagacgat gcac 44 <210> 560 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1432 gene <400> 560 agctggatca tagagccatt cat 23 <210> 561 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1433 gene <400> 561 tagacagatg cttgagtggt cta 23 <210> 562 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1433 gene <400> 562 gacgaactcc aattcactgt ttcagcaatc tggcttggcg acat 44 <210> 563 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1433 gene <400> 563 agatttagat gtctaaaaag caccagtgct gacttgatta agga 44 <210> 564 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1433 gene <400> 564 gtgcatcgtc ttgtgaagct gat 23 <210> 565 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1434 gene <400> 565 atgcttatag ctacgctgtt cgt 23 <210> 566 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1434 gene <400> 566 gacgaactcc aattcactgt ttgtattagc caagtggcgc aaca 44 <210> 567 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1434 gene <400> 567 agatttagat gtctaaaaag ctagacagat gcttgagtgg tcta 44 <210> 568 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1434 gene <400> 568 tcagcaatct ggcttggcga cat 23 <210> 569 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1439 gene <400> 569 gatgctcgca aattggctgc tga 23 <210> 570 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1439 gene <400> 570 gacgaactcc aattcactgt tactattgat aatgcaggca ttgc 44 <210> 571 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1439 gene <400> 571 agatttagat gtctaaaaag cagtaccgtt gttgggattc agct 44 <210> 572 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1439 gene <400> 572 agattatagg catctatagc tgt 23 <210> 573 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1458 gene <400> 573 ctggtctcca gtttctgaac gtc 23 <210> 574 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1458 gene <400> 574 gacgaactcc aattcactgt tcacaggcga tacatcgttc aggt 44 <210> 575 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1458 gene <400> 575 agatttagat gtctaaaaag ctagtcagga tgtcttagag gctg 44 <210> 576 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1458 gene <400> 576 atgcaggctt gcaatatgcg aca 23 <210> 577 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1460 gene <400> 577 aggacaacta cgagattatc agc 23 <210> 578 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1460 gene <400> 578 gacgaactcc aattcactgt tacaacttga tgacctgctt ggat 44 <210> 579 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1460 gene <400> 579 agatttagat gtctaaaaag cacggtcgta ctaagctcat gcta 44 <210> 580 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1460 gene <400> 580 tgtcgcatat tgcaagcctg cat 23 <210> 581 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1468 gene <400> 581 tgatgctaat accagaatcc atg 23 <210> 582 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1468 gene <400> 582 gacgaactcc aattcactgt ttagcgtatc tggtgtataa gcac 44 <210> 583 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1468 gene <400> 583 agatttagat gtctaaaaag catgcagatt atgctattgg attg 44 <210> 584 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1468 gene <400> 584 tgtgctcatc gaacactgtg tca 23 <210> 585 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1472 gene <400> 585 catggattct ggtattagca tca 23 <210> 586 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1472 gene <400> 586 gacgaactcc aattcactgt tatcttcagt gatagcgcga gtag 44 <210> 587 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1472 gene <400> 587 agatttagat gtctaaaaag ccttgatgaa atgggcgtgg acta 44 <210> 588 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1472 gene <400> 588 agtacaccaa ggaaggtggt ctg 23 <210> 589 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1502 gene <400> 589 acacgtcagt tctctcaagc aga 23 <210> 590 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1502 gene <400> 590 gacgaactcc aattcactgt ttgctcgatt ggcagaccat gagt 44 <210> 591 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1502 gene <400> 591 agatttagat gtctaaaaag ctcaagatac agtcgcttac gatg 44 <210> 592 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1502 gene <400> 592 tctacgatgc ttgcacagtg gtc 23 <210> 593 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1510 gene <400> 593 accactagca atggttcagt ctg 23 <210> 594 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1510 gene <400> 594 gacgaactcc aattcactgt tcttcgtcga ccatacggtt gatg 44 <210> 595 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1510 gene <400> 595 agatttagat gtctaaaaag cgtagacact ctattgatta tctc 44 <210> 596 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1510 gene <400> 596 agagaccgaa ccactctctc gat 23 <210> 597 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1511 gene <400> 597 gtactcttgc ttcctggtca atc 23 <210> 598 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1511 gene <400> 598 gacgaactcc aattcactgt ttgctgctgc atcaatatca acaa 44 <210> 599 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1511 gene <400> 599 agatttagat gtctaaaaag caccactagc aatggttcag tctg 44 <210> 600 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1511 gene <400> 600 cttcgtcgac catacggttg atg 23 <210> 601 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1514 gene <400> 601 tggcttatcc agtcttcact aag 23 <210> 602 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1514 gene <400> 602 gacgaactcc aattcactgt ttctcagaca aggttactgc tgca 44 <210> 603 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1514 gene <400> 603 agatttagat gtctaaaaag cagccatcgt gaccttggtt ggag 44 <210> 604 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1514 gene <400> 604 agtcttcgta gcgctcacgg tca 23 <210> 605 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1515 gene <400> 605 gttctatctg tactgatccg act 23 <210> 606 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1515 gene <400> 606 gacgaactcc aattcactgt taccactgca ccttcttcgt agat 44 <210> 607 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1515 gene <400> 607 agatttagat gtctaaaaag ctggcttatc cagtcttcac taag 44 <210> 608 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1515 gene <400> 608 tctcagacaa ggttactgct gca 23 <210> 609 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1535 gene <400> 609 actattaacc ttgtcttctc cta 23 <210> 610 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1535 gene <400> 610 gacgaactcc aattcactgt ttaccatcga tagcaatcga acct 44 <210> 611 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1535 gene <400> 611 agatttagat gtctaaaaag cacagttggt tcatttgaga gctg 44 <210> 612 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1535 gene <400> 612 actacgtacg tagaagttca aga 23 <210> 613 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1540 gene <400> 613 gattcgaatt gttcgccaga cct 23 <210> 614 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1540 gene <400> 614 gacgaactcc aattcactgt tcatcttgaa ttgccttggc aacg 44 <210> 615 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1540 gene <400> 615 agatttagat gtctaaaaag cctactgctg atgaggaatc agat 44 <210> 616 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1540 gene <400> 616 ctggacacta gcaggagtca cta 23 <210> 617 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1541 gene <400> 617 agttgcacta ccgcgtgatt aca 23 <210> 618 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1541 gene <400> 618 gacgaactcc aattcactgt tatcgaagcc aattcttcga tgtc 44 <210> 619 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1541 gene <400> 619 agatttagat gtctaaaaag cgtctatcag ccacaccgat cagt 44 <210> 620 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1541 gene <400> 620 cagctggaac tgtcttgaca tga 23 <210> 621 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1553 gene <400> 621 tagaaggtct cttgattagt tc 22 <622> 622 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1553 gene <400> 622 gacgaactcc aattcactgt tctacgtatt cgtctgcatc ggta 44 <210> 623 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1553 gene <400> 623 agatttagat gtctaaaaag cgcagttcca atacctgcct tacg 44 <210> 624 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1553 gene <400> 624 aggtaccata agccacctcc tac 23 <210> 625 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1555 gene <400> 625 acctgtctca ctcaatcaag tct 23 <210> 626 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1555 gene <400> 626 gacgaactcc aattcactgt ttctgaacta aatcttgata gtga 44 <210> 627 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1555 gene <400> 627 agatttagat gtctaaaaag cacttatatg gagactttgg tgtg 44 <210> 628 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1555 gene <400> 628 gaactaatca agagaccttc ta 22 <210> 629 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1571 gene <400> 629 ctatctaagg agagaagcaa ggt 23 <210> 630 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1571 gene <400> 630 gacgaactcc aattcactgt ttagcactgg ccgccaaggt cacg 44 <210> 631 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1571 gene <400> 631 agatttagat gtctaaaaag cgaccagtct attctctaca gtca 44 <210> 632 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1571 gene <400> 632 tatctgctcg acatgcagat cac 23 <210> 633 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1580 gene <400> 633 atccgcagca ctatgctatt cga 23 <210> 634 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1580 gene <400> 634 gacgaactcc aattcactgt ttcaaggcat aatggatagg cgca 44 <210> 635 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1580 gene <400> 635 agatttagat gtctaaaaag catccaggtg agattctctc tatc 44 <210> 636 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1580 gene <400> 636 caggcatcaa gcactcgctc atc 23 <210> 637 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1603 gene <400> 637 tacctatata gatgccagca aga 23 <210> 638 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1603 gene <400> 638 gacgaactcc aattcactgt tagacgacca aggctgattg atgt 44 <210> 639 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1603 gene <400> 639 agatttagat gtctaaaaag cctgacaagc tctgtgtcga gtca 44 <210> 640 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1603 gene <400> 640 caatcgctgg tggagttctt gca 23 <210> 641 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1617 gene <400> 641 agctatgaag ccagaagcgg atg 23 <210> 642 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1617 gene <400> 642 gacgaactcc aattcactgt tatcattgat ccaaccaatc tcag 44 <210> 643 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1617 gene <400> 643 agatttagat gtctaaaaag cgacgctatt actccgtagt agct 44 <210> 644 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1617 gene <400> 644 cgtgatagct cagctggcac cgt 23 <210> 645 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1632 gene <400> 645 cagcaggagc cgactatatc ggt 23 <210> 646 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1632 gene <400> 646 gacgaactcc aattcactgt tgttgcagtt gccacaccgt gctg 44 <210> 647 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1632 gene <400> 647 agatttagat gtctaaaaag ccagccttgg aatctagcca tgct 44 <210> 648 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1632 gene <400> 648 acttgatgaa gttgcgccaa gtg 23 <210> 649 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1642 gene <400> 649 gctgcgacaa cagcaacaat tct 23 <210> 650 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1642 gene <400> 650 gacgaactcc aattcactgt ttgatataac ttgagctcct aatc 44 <210> 651 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1642 gene <400> 651 agatttagat gtctaaaaag ctgaacatct tcgactgatg gaag 44 <210> 652 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1642 gene <400> 652 tggaatatac gacaattcct cta 23 <210> 653 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1647 gene <400> 653 catgtaccaa tggctattac ctc 23 <654> 654 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1647 gene <400> 654 gacgaactcc aattcactgt ttactacggt atggctacca atat 44 <210> 655 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1647 gene <400> 655 agatttagat gtctaaaaag ctgcacaagt agcggtaggt aagc 44 <210> 656 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1647 gene <400> 656 tgtatctaga ggaagattac tgc 23 <210> 657 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1666 gene <400> 657 gctctattct aactcatgat cat 23 <210> 658 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1666 gene <400> 658 gacgaactcc aattcactgt tcgtcaccac aacgtgacca tctc 44 <210> 659 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1666 gene <400> 659 agatttagat gtctaaaaag cagaatgtgt ccatcaaggt cgta 44 <210> 660 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1666 gene <400> 660 ctggatctct aacttgtcca tct 23 <210> 661 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1671 gene <400> 661 actatcaatc tgttagcgag tct 23 <210> 662 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1671 gene <400> 662 gacgaactcc aattcactgt ttaataccga atcgtatccg ctga 44 <210> 663 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1671 gene <400> 663 agatttagat gtctaaaaag cagcgcttgt ctcagctaca gaca 44 <210> 664 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1671 gene <400> 664 tgcgtctgct cctccgccga gga 23 <665> 665 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1674 gene <400> 665 atcattgagc ttgcgaatga cag 23 <210> 666 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1674 gene <400> 666 gacgaactcc aattcactgt tcatacgacg gtaaccacct gaca 44 <210> 667 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1674 gene <400> 667 agatttagat gtctaaaaag ctcatgcagc caattggact tcta 44 <210> 668 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1674 gene <400> 668 agaccatatc accagtctgc tat 23 <210> 669 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1696 gene <400> 669 atgaagatgc agaagtgtct gag 23 <210> 670 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1696 gene <400> 670 gacgaactcc aattcactgt ttgaccagct gccaagtata ttca 44 <210> 671 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1696 gene <400> 671 agatttagat gtctaaaaag ccagtgttac caagaaggtg gtct 44 <210> 672 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1696 gene <400> 672 acgagctgac cagacgcctg gta 23 <210> 673 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1697 gene <400> 673 ctgatcgtca ggaattatcc atg 23 <210> 674 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1697 gene <400> 674 gacgaactcc aattcactgt tctagcacaa tcaatacaat tgct 44 <210> 675 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1697 gene <400> 675 agatttagat gtctaaaaag cagcgaagct aaggtacaac aagt 44 <210> 676 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1697 gene <400> 676 agaccacctt cttggtaaca ctg 23 <210> 677 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1776 gene <400> 677 cgttcagtcg gaccttactc aac 23 <210> 678 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr 1776 gene <400> 678 gacgaactcc aattcactgt ttcatgatag acttgtgctc aagt 44 <210> 679 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1776 gene <400> 679 agatttagat gtctaaaaag cacgtacctt ctcggtgaag taca 44 <210> 680 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1776 gene <400> 680 gactcttctg aatctgaacc atg 23 <210> 681 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1797 gene <400> 681 actatgtgga gagtgatggc cag 23 <210> 682 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1797 gene <400> 682 gacgaactcc aattcactgt tcataatctg ctgccagaat tgac 44 <210> 683 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1797 gene <400> 683 agatttagat gtctaaaaag catcaagcac gtccttcatc tagt 44 <210> 684 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1797 gene <400> 684 gtagtctgaa cgaggttcta gct 23 <210> 685 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1804 gene <400> 685 tacacataca cacttgaatg tag 23 <210> 686 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1804 gene <400> 686 gacgaactcc aattcactgt ttgagattga ccgtatcatc acga 44 <210> 687 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1804 gene <400> 687 agatttagat gtctaaaaag catcggctat tctaacggca agca 44 <210> 688 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1804 gene <400> 688 cacattcctg aaataagagt atc 23 <210> 689 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1825 gene <400> 689 gatgacatgg aggacagcaa cag 23 <210> 690 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1825 gene <400> 690 gacgaactcc aattcactgt tatctgtaag gtcgttgatg cgtg 44 <210> 691 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1825 gene <400> 691 agatttagat gtctaaaaag cagttatctc aggtgcttca tgta 44 <210> 692 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1825 gene <400> 692 acttccttgg ctcattgcaa gct 23 <210> 693 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1853 gene <400> 693 gtcatattgg taacggaggc tca 23 <210> 694 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1853 gene <400> 694 gacgaactcc aattcactgt ttgctaactg atagacctac tcga 44 <210> 695 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1853 gene <400> 695 agatttagat gtctaaaaag catgtcgact ttgttgtcat tgct 44 <210> 696 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1853 gene <400> 696 gataatcttg aatggattat tca 23 <210> 697 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1856 gene <400> 697 aggcaggtgt tctcctctac gca 23 <210> 698 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1856 gene <400> 698 gacgaactcc aattcactgt ttcttgattg cttgctgctc cact 44 <210> 699 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1856 gene <400> 699 agatttagat gtctaaaaag ctctgtgcat agtcagattc tcta 44 <210> 700 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1856 gene <400> 700 atagcgtatc cgtctgactt gag 23 <210> 701 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1881 gene <400> 701 agcttaccaa tacgcagctg ttc 23 <210> 702 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1881 gene <400> 702 gacgaactcc aattcactgt ttcagtatct tcgatacgga tgag 44 <210> 703 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1881 gene <400> 703 agatttagat gtctaaaaag cgtcacatga ccttgattat caac 44 <210> 704 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1881 gene <400> 704 cttctactgc agctacaaca tga 23 <210> 705 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1882 gene <400> 705 gtgatgagtc agttgtacgt gat 23 <210> 706 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1882 gene <400> 706 gacgaactcc aattcactgt ttgctgctgt tacttgtgat tgca 44 <210> 707 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1882 gene <400> 707 agatttagat gtctaaaaag cagcttacca atacgcagct gttc 44 <210> 708 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1882 gene <400> 708 tcagtatctt cgatacggat gag 23 <210> 709 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1910 gene <400> 709 atagaagaga tgtccttgac ctg 23 <210> 710 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1910 gene <400> 710 gacgaactcc aattcactgt tcaatatcac ggaggaagtc aatg 44 <210> 711 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1910 gene <400> 711 agatttagat gtctaaaaag cgtcacttga tgagattgaa gata 44 <210> 712 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1910 gene <400> 712 tgactcagcg gctctaactc tat 23 <210> 713 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1914 gene <400> 713 cgaagtcgtc attgcaatgc tga 23 <210> 714 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1914 gene <400> 714 gacgaactcc aattcactgt ttcatggtta ccaatataga ggca 44 <210> 715 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1914 gene <400> 715 agatttagat gtctaaaaag cagaactatg attgactggc tact 44 <210> 716 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1914 gene <400> 716 catggatatc ctgcttgata tct 23 <210> 717 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1950 gene <400> 717 tctatgaggt ggtgacacga cca 23 <210> 718 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1950 gene <400> 718 gacgaactcc aattcactgt tagcgttgat ctagccacgc tag 43 <210> 719 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1950 gene <400> 719 agatttagat gtctaaaaag ctgtcaagaa gccattgagc gcat 44 <210> 720 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1950 gene <400> 720 tcgccaatga gaagaatgtc ctc 23 <210> 721 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1963 gene <400> 721 tacaggtcaa gctactcgag atg 23 <210> 722 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1963 gene <400> 722 gacgaactcc aattcactgt ttgccttcga tgtacttatc tgtc 44 <210> 723 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1963 gene <400> 723 agatttagat gtctaaaaag ctatgtgatt actgatgaag aacg 44 <210> 724 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1963 gene <400> 724 tgtactcagt gtgactatca cca 23 <210> 725 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1971 gene <400> 725 gatatgagga ggacaaggat gtc 23 <210> 726 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1971 gene <400> 726 gacgaactcc aattcactgt tcacatgagg cagaaggata attc 44 <210> 727 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1971 gene <400> 727 agatttagat gtctaaaaag cagcttatgc tattgttgct acag 44 <210> 728 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1971 gene <400> 728 tgtcaccagt aatgcttgtg tga 23 <210> 729 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1973 gene <400> 729 tagcttggct gagagaagct tca 23 <210> 730 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1973 gene <400> 730 gacgaactcc aattcactgt tcaacgattg ctcttccaga agtc 44 <210> 731 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1973 gene <400> 731 agatttagat gtctaaaaag cagaggagta tggtttaggc gata 44 <210> 732 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1973 gene <400> 732 gacatccttg tcctcctcat atc 23 <210> 733 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr1987 gene <400> 733 agcaatagct caggttggat tgt 23 <210> 734 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr1987 gene <400> 734 gacgaactcc aattcactgt ttcggcacgt tgcttgtagg tgct 44 <210> 735 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr1987 gene <400> 735 agatttagat gtctaaaaag ctatgcttac aaattacttg agca 44 <210> 736 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr1987 gene <400> 736 tgcgaggata atagcttctg tgt 23 <210> 737 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr2012 gene <400> 737 tacatggaga atcggaacct tat 23 <210> 738 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr2012 gene <400> 738 gacgaactcc aattcactgt tagagtcatc attcctcaga agta 44 <210> 739 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr2012 gene <400> 739 agatttagat gtctaaaaag cagtcctgat agatatgcta atag 44 <210> 740 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr2012 gene <400> 740 atcacattgg ttgtctgatc ttc 23 <210> 741 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr 2013 gene <400> 741 tacaggaact tcaacctctc gta 23 <210> 742 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr 2013 gene <400> 742 gacgaactcc aattcactgt tgatcacttc tgactcgctt catc 44 <210> 743 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr 2013 gene <400> 743 agatttagat gtctaaaaag ctacatggag aatcggaacc ttat 44 <210> 744 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr 2013 gene <400> 744 agagtcatca ttcctcagaa gta 23 <210> 745 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr2027 gene <400> 745 tacgattagc tagaatcaat cag 23 <746> 746 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr2027 gene <400> 746 gacgaactcc aattcactgt tatagctgca actatatgac tctc 44 <210> 747 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr2027 gene <400> 747 agatttagat gtctaaaaag ctatctgtcg tgagttagct gtga 44 <210> 748 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr2027 gene <400> 748 tgaccaatac gcgatcactc atg 23 <210> 749 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr2034 gene <400> 749 ctcgtccaag agaagaacat ctg 23 <750> 750 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr2034 gene <400> 750 gacgaactcc aattcactgt tatatcatac ttatcctctt cctg 44 <210> 751 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr2034 gene <400> 751 agatttagat gtctaaaaag ctgtgatgtc ttggtagagc cgga 44 <210> 752 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr2034 gene <400> 752 tgactctctg taactgtgtc cat 23 <210> 753 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying left flanking region of spr0476 gene <400> 753 catcagtgga aggaatggtt gacc 24 <210> 754 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying left flanking region of spr0476 gene <400> 754 gacgaactcc aattcactgt tatctaccca caagagcttg a 41 <210> 755 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> forward primer for amplifying right flanking region of spr0476 gene <400> 755 agatttagat gtctaaaaag ccatgaaaag cgtcgtttga c 41 <210> 756 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for amplifying right flanking region of spr0476 gene <400> 756 gttgcgattg cgtccacctc ctca 24

Claims (9)

Essential genes of Streptococcus pneumoniae , spr0003, spr0047, spr0081, spr0129, spr0131, spr0139, spr0145, spr0156, spr0173, spr0210, spr0243, spr0266, spr0267, spr0268, spr02r, spr02 spr0393, spr0395, spr0478, spr0530, spr0585, spr0604, spr0626, spr0646, spr0666, spr0670, spr0671, spr0729, spr0755, spr0756, spr0788, spr0796, spr0797, spr0845, spr0856, 08 spr0978, spr0979, spr0982, spr0992, spr0996, spr1001, spr1024, spr1036, spr1063, spr1064, spr1065, spr1081, spr1111, spr1150, spr1158, spr1282, spr1312, spr1329, spr13r, spr13, 136 spr1411, spr1413, spr1419, spr1427, spr1429, spr1430, spr1432, spr1433, spr1434, spr1439, spr1458, spr1460, spr1468, spr1472, spr1502, spr1510, spr1511, spr1514, spr1515, sp115r spr1580, spr1603, spr1617, spr1632, spr1642, spr1647, spr1666, spr1671, spr1674, s spr2027, spr2027, spr2027, spr2027, spr2027, spr2097, spr1697, spr1776, spr1797, spr1804, spr1825, spr1853, spr1856, spr1881, spr1882, spr1910, spr1914, spr1950, spr1963, spr1971, spr1973, spr1987, spr2012, spr2013, and spr2027 Compositions for Screening Materials The method of claim 1, SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 223, 225, 227, 229, 231, 233, 235, 237, 239, 241, Nucleotide sequences described in 243, 245, 247, and 249, or sequences in which one or more disruptions, deletions, insertions, dots, substitutions, nonsense, missense, polymorphisms, or rearrangement mutations have occurred in the nucleotide sequences of these SEQ ID NOs: Antibiotic screening composition comprising one or more selected from Composition for antibiotic screening comprising one or more selected from proteins expressed from essential genes of pneumococcal according to claim 1 or 2 The method of claim 3, wherein SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, One or more disruptions, deletions, insertions, points, substitutions, nonsense, missenses, polymorphisms, or rearrangement mutations in the amino acid sequence of 244, 246, 248, 250, or the nucleotide sequence of the essential genes of claim 2 Composition for screening antibiotics comprising at least one selected from polypeptide fragments expressed from nucleotide sequences An antibiotic screening method using the composition of claim 1 or 2 as a target material The method according to claim 5, wherein the contact with the test substance after contacting the composition and confirming the reaction therebetween, comprising the step of determining whether the composition exhibits the activity of inhibiting the expression of the genes included Screening method An antibiotic screening method using the composition of claim 3 or 4 as a target material 8. The antibiotic substance of claim 7, wherein the composition comprises contacting the test substance with a test substance and confirming a reaction therebetween to determine whether the composition exhibits an activity that inhibits the function of the protein. Screening method 9. The antibiotic screening method of claim 6 or 8, wherein the chemical library is screened.