KR200482473Y1 - Phytoremediation Pot for soils contaminated with explosive compounds - Google Patents

Abstract

According to the present invention, the ground-type port for flora purification of a soil contaminated with the explosive substance is a body made of a biodegradable material having a waterproof function and filled with soil in the upper and lower openings; A mesh provided at a lower portion of the body; A cover provided so as to be detachable to the lower portion of the mesh and to prevent water and air from passing therethrough and preventing the soil from flowing out; And a plant for purifying the explosive substance which is planted in the soil and absorbs and removes the explosive substance into the living body.

Description

BACKGROUND OF THE INVENTION Field of the Invention [0001] The present invention relates to a phytoremediation pot for soils contaminated with explosives,

The present invention relates to a ground-type pot for the purification of soil in a soil contaminated with an explosive substance that can be planted in the field by a plant for the disinfection of an explosive substance in a time-dependent manner even if it is placed on the ground site contaminated with an explosive substance.

Gunpowder substances are frequently detected environmental pollutants in soil, groundwater, sediment and water around gunpowder production / use facilities such as military shooting ranges, gunpowder production plants, and waste disposal plants. TNT (2,4,6-Trinitrotoluene ), RDX (Royal Demolition eXplosive, Hexahydro-1,3,5-trinitro-1,3,5-triazine) and HMX (High Melting Explosive, Octahydro-1,3,5,7-tetranitro- , 7-tetrazocine) are detected with the highest frequency and concentration.

These gunpowder materials are highly oxidized materials and are not only structurally biodegradable materials, but they are toxic and adversely affect humans and ecosystems when released into the natural environment. Ingestion of gunpowder substances in the human body can have adverse effects such as carcinogenesis, neurological disorders, hematopoietic dysfunction, diminished digestive function, and can seriously harm all ecosystems including soil and aquatic microorganisms, invertebrates and plants and animals, Of these, TNT and RDX are classified as Class C carcinogens by the US Environmental Protection Agency (EPA) and are strictly controlled.

In general, incineration or activated carbon adsorption, which is a physicochemical treatment method, has been widely used as a method for purifying soil or ground water contaminated with explosive materials such as TNT and RDX. However, incineration has the problem of not only high treatment cost and loss of soil properties but also formation of secondary pollutants such as gaseous pollutants and bottom ash. In addition, the activated carbon adsorption method has a high treatment cost, and there is a problem that the activated carbon containing the powdered substance after the treatment must be reprocessed.

On the other hand, there is a biological purification method (bioremediation) for purifying the soil or ground water contaminated with the explosive substance using microorganisms. However, when the microorganism decomposing the explosive substance substance is expensive and the microorganism is actually applied to the contaminated site, It is often reported that biomass falls rapidly because of competing food or food conditions and indigenous bacteria that can not effectively degrade pollutants. In addition, there is also a case where the degradation products of explosive substances by microorganisms are highly toxic, and there is a possibility of inducing another secondary pollution.

Recently, as a method for purifying the area contaminated with the explosive substance, research on a flora purification method using a wide green area near the shooting range has been actively carried out. Phytoremediation uses plants to remove pollutants in soil, water, and groundwater by decomposing, absorbing, stabilizing, accumulating, lyophilization, and evapotranspiration, or by reducing the mobility and reducing the risk of pollutants. Green technology is more economical and environmentally friendly than bioremediation.

Particularly, when a flora purification method is applied to purify soil contaminated with organic toxic substances, organic matter has a short ring structure and only when the value of LogKow (log octanol: water partition coefficients) is 0.5 to 3.0, It is absorbed effectively, the gunpowder material belongs to this category. Since removal efficiency is influenced by the physicochemical characteristics of the soil, the absorption and resolution of the plant, and the physiological effects of the plant, it is most important to select the appropriate plant species for the successful flour cleansing method.

On the other hand, the site site contaminated with gunpowder is a dangerous area in which there are unexploded bombs such as a shooting range. Therefore, when planting a gunpowder purification plant on a site contaminated with a gunpowder material, it should be possible to plant it quickly and safely. There is no technology that reflects this.

The present invention has been made to overcome the limitations and problems of the prior art as described above, and it is an object of the present invention to provide a method of disposing a disinfectant for disinfecting a disinfectant, The present invention provides a ground-type port for flora purification of contaminated soil and a flora purification method using the same.

In order to achieve the above object, the ground-type pot for purifying flora of a soil contaminated with the explosive substance according to the present invention comprises: (1) a body made of a biodegradable material which is opened upward and downward and filled with soil in an inner space, (2) a mesh provided at a lower portion of the body, (3) a cover which is detachably attached to the lower portion of the mesh, and which allows water and air to pass therethrough but prevents the soil from flowing out, (4) And a plant for purifying explosive substances that absorbs and removes the explosive substance in vivo.

At this time, the body is made of paper coated with an oily material, and the cover is preferably made of a nonwoven fabric.

In addition, the body may be formed in a tapered shape whose lower portion is wider than the upper portion.

Particularly, it is preferable that the plant for purifying the explosive substance is Galp or Urea.

On the other hand, a flora remediation method for a soil contaminated with explosive materials includes (1) a body made of a biodegradable material opening up and down and having a waterproof function, a mesh provided at a lower portion of the body, A first step of planting a plant for purifying a gunpowder material, which is filled with soil in a body of a pot each of which is equipped with a cover for preventing water and air from passing through and a cover for preventing soil from flowing out, And a second step of removing the cover from the port and disposing the port in a zone contaminated with the explosive material.

The ground-type pot for the flora purification of the soil contaminated with the explosive substance material according to the present invention and the flora purification method using the same are arranged in the field site contaminated with the explosive substance such as a shooting range, Not only is the plant planted on site, but also because the body is made of biodegradable material so that it is naturally decomposed with time after being placed on the site, soil contamination by the port itself can be prevented.

Also, according to the present invention, the ground-type pot for the flora purification of the soil contaminated with the explosive substance and the flora purification method using the same have a cover detachable at the lower part of the mesh, so that the plant for purifying the explosive substance- It is only necessary to dispose the port on site with the cover removed, so that plants for the disinfection of the explosive substances can be planted quickly and safely in the contaminated site.

Particularly, by using the ground-type port for flora purification and the flora purification method using the domestic flora of the domestic native grassland according to the present invention of planting gall pillow or kelp, it is possible to absorb the powdered substance into the plant, Since the explosive substance absorbed into the plant is reduced by the biological conversion process by the enzymatic system in the plant, the pollutant can be removed continuously and stably even at a relatively high concentration.

In other words, Gallup and Urea are perennial plants, adapted from domestic climate and soil, and are indigenous plants inhabited in Korean mountains and waterfronts. Therefore, it is possible to prevent damage or disturbance of natural ecosystem that may occur when foreign plants are introduced. In addition, Gallup and Urea are perennial high biomass plants, and when they are planted once, they grow into rhizomes and form communities. So it is easy to cultivate single species and absorbs a large amount of water, so that it can absorb a large amount of explosive materials, especially RDX and HMX, The removal of explosive substances per unit biomass is very advantageous. Therefore, by using the ground-type port for flora purification and the flora purification method using the polluted material with the gunpowder or cormorant according to the present invention, it is possible to remove the explosive substance in the process of growing the plant, It can protect the ecosystem around the area.

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a top view of a ground-type port for flora purification of a soil contaminated with a gunpowder material according to one embodiment of the present invention.
FIG. 2 is a photograph of a gallop planted on a shooting range in Gyeonggi-do in Feb. 23, 2013.
FIG. 3 is a photograph of a gallop planted on a shooting range in Gyeonggi-do in three months from March 23, 2013.
Fig. 4 is a photograph of the cormorant planted at the shooting range of Gyeonggi-do on March 4, 2013.
FIG. 5 is a photograph of the cormorant planted on the shooting range of Gyeonggi-do, Korea, on March 4, 2013, about three months after.
Fig. 6 is a graph showing the biomass of the treated grouping gall and the dry weight thereof after the ending of the galliform growth.
FIG. 7 is a graph showing the change in ground level of gallphil during the period of growth of gallophora.
FIG. 8 is a graph showing soil / plant explosive substance concentrations of TNT and RDX treatments by Gallup.
Figure 9 is a graph showing the mass balance of TNT and RDX for a galloping plant.
FIG. 10 is a graph showing acute toxicity of early and final soils in trout farms.
Fig. 11 is a graph showing the biomass and the dry weight of the seagrass cormorant after the end of cultivation of the cormorant.
Fig. 12 is a graph showing the change in the ground level of the hawthorn wheat during the period of growing the marrow.
13 is a graph showing soil / plant explosive substance concentrations of TNT and RDX treatments due to cormorant;
Fig. 14 is a graph showing the mass balance of TNT and RDX for a wheatgrass plant.
15 is a graph showing the acute toxicity of the initial and final soils in the fieldbore plant.
FIG. 16 is a photograph of a gallop planted in a ground-type port for flora purification according to an embodiment of the present invention as seen from a greenhouse.
FIG. 17 is a photograph of a ground-type port for flora purification according to an embodiment of the present invention in which a gallop disposed at the 2013. 9. 28. A shots is disposed.
FIG. 18 is a photograph of a ground-type port for flora purification according to an embodiment of the present invention in which a gallop disposed at a landing site of A in the 2013. 9. 28. month is planted about two months later.
FIG. 19 is a photograph of a ground-type port for flora purification according to an embodiment of the present invention in which a gallop disposed at the A-shooting range is located on the 2013. 9. 28. place.
FIG. 20 is a photograph of a ground-type port for flora purification according to an embodiment of the present invention in which a gallop disposed at a landing site of A, A, F,

The above objects and advantages of the present invention will become more apparent from the following detailed description in conjunction with the accompanying drawings, and it is believed that those skilled in the art will readily understand the technical idea of the present invention . In the following description of the present invention, a detailed description of known technologies related to the present invention will be omitted when it is determined that the gist of the present invention may unnecessarily obscure the present invention.

Furthermore, terms used herein are for the purpose of illustrating embodiments and are not intended to limit the present invention. In this specification, the singular forms include plural forms as the case may be, unless the context clearly indicates otherwise. &Quot; comprises "and / or" comprising "used in the specification do not exclude the presence or addition of one or more other elements other than the stated element. Unless otherwise defined, all terms used herein may be used in a sense that is commonly understood by one of ordinary skill in the art to which this invention belongs. In addition, commonly used predefined terms are not ideally or excessively interpreted unless explicitly defined otherwise.

Hereinafter, preferred embodiments according to the present invention will be described in detail with reference to the accompanying drawings.

1 shows a ground type port 100 for flora purification of a soil contaminated with an explosive substance according to one embodiment of the present invention.

1, the ground type port 100 for flora purification of a soil contaminated with the explosive substance material according to one embodiment of the present invention is a pot containing a plant for purifying the explosive substance which absorbs and removes the explosive substance in vivo, The body 10, the mesh 20, the cover 30, and the plant 40 for treating the explosive substance.

Specifically, the body 10 has an inner space and is vertically opened. The inner space is filled with the soil 11 so that the plant 40 is planted. Particularly, the body 10 is preferably made of a biodegradable material. This allows the body 10 to be disassembled even in a natural state over time when the ground-type port 100 for flora purification according to an embodiment of the present invention is disposed at the site where the explosive material is contaminated, In order to prevent contamination of the site at the site.

Particularly, the body 10 is preferably made of a biodegradable material having a waterproof function. This is to prevent the body 10 from being disassembled or deformed by the water supplied in the course of cultivating the plant 40 for treating the explosive substance purifying plant 40 so that the function as a case is not deteriorated. Specifically, the body 10 is made of papers (for example, oil type or coated corrugated cardboard) coated with an oily material such as vegetable oil having excellent waterproofing properties and biodegradability, biodegradable plastic, biodegradable resin, etc. . At this time, the body 10 can more safely cultivate the plant 40 for pottery purification, and the ground-type port 100 for flora purification according to one embodiment of the present invention can be well settled on the ground contaminated with the explosive substance It is preferable that the lower portion is formed in a tapered shape wider than the upper portion.

The mesh 20 is a plate having a plurality of holes and is provided at a lower portion of the body 10 to primarily support the earth 11 filled in the body 10. Accordingly, the mesh 20 is preferably made of a material having a higher toughness than the body 10, and may be made of, for example, a plastic material. In addition, the mesh 20 acts to allow the roots of the plant 40 to be well settled in the field. That is, when the ground-type port 100 for flora cleaning according to an embodiment of the present invention is disposed at the site where the explosive material is contaminated, a plurality of perforated meshes 20 are provided at the bottom of the body 10 The plant 40 for purifying the explosive substance is settled by its roots growing well into the ground through a plurality of holes of the mesh 20. [

Since the mesh 20 has a plurality of openings, when the ground-type port 100 for flora purification according to an embodiment of the present invention is moved, the soil 11 may flow out through the mesh 20 have. Accordingly, the ground type port 100 for flora purification according to an embodiment of the present invention includes a cover 30 provided at a lower portion of the mesh 20. That is, the cover 30 prevents the soil 11 from flowing out of the mesh 20 while secondarily supporting the soil 11 filled in the body 10. At the same time, it is preferable that the cover 30 is made of a material that allows water and air to pass through well so that the roots of the explosive substance purifying plant 40 are not decayed in the process of growing the explosive substance purifying plant 40. For example, the cover 30 may be made of a material such as a nonwoven fabric which is ventilated and has a high toughness. Particularly, in order to prevent contamination of the ground site by the cover 30, the cover 30 is removed before the ground-type port 100 for flora cleaning according to an embodiment of the present invention is disposed on the site contaminated with the explosive substance Be able to. Accordingly, it is preferable that the cover 30 is configured to be detachable from the lower portion of the mesh 20.

On the other hand, the site site contaminated with gunpowder is a dangerous area in which there are unexploded bombs such as a shooting range. Therefore, when planting a gunpowder purification plant (40) on a site contaminated with a gunpowder material, do. In the case of using the ground-type port 100 for flora purification according to an embodiment of the present invention having the cover 30 detachably attachable to the lower portion of the mesh 20, It is only necessary to dispose the port 100 in the field without removing the cover 30, so that the user can quickly and safely plant the explosive substance purifying plant 40 in the contamination site.

A plant 40 for purifying the explosive substance is a plant which absorbs and removes explosive substance in vivo, and is planted and cultivated in the soil 11 of the body 10. In the present invention, the explosive substance to be purified is an aromatic compound containing a nitro group, preferably TNT (2,4,6-Trinitrotoluene), RDX (Royal Demolition eXplosive, Hexahydro-1,3,5-trinitro -1,3,5-triazine) and HMX (High Melting Explosive, Octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine). Specifically, the plant 40 for purifying the explosive substance may be an eagle or a red mullet, but it is preferably a gall pill or a mulberry. This is because it is a plant that is optimized for the following conditions that can perform the flora purification method for gunpowder material on the site where the gallop and the marl are contaminated with gunpowder.

- Compliance condition

(i) It should be a plant that is adaptable to domestic soil and climate and is capable of growing in any area, and is particularly applicable to field applications that are contaminated with explosives materials.

(ii) It is a perennial plant that grows over a long period of time; it should be a plant that grows rhizomes rather than seeds.

(iii) A plant that can decompose after absorption, not merely absorbing the explosive substance, and incorporating the conversion product into the plant.

(iv) Plants should be capable of decomposing explosive materials by activating microorganisms by organic matter emitted from the rhizosphere of the plant.

Fig. 2 is a photograph of a gallop planted near the shooting area of Gyeonggi-do on March 23, 2013, and Fig. 3 is a photograph of a gallop that was planted as shown in Fig. 2 after three months. FIG. 4 is a photograph of the sea grass planted near the shooting area of Gyeonggi-do in Jan. 4, 2013, and FIG. 4 is a photograph of the three months after the planting of the seaweed as shown in FIG.

With respect to the compliance condition (i) above, the plant 40 for potash remediation should be well adapted to the satellites, climatic and terrain conditions of the application area. In particular, sites such as shooting ranges contaminated with gunpowder are barren areas where nutrients are insufficient, so the potash material purification plant (40) must have field applicability that can be applied in the field of barren soils.

In order to confirm the applicability of the galloping to the field, as shown in FIG. 2, a gallop was planted near the pit area of the shooting range of Gyeonggi A, which is currently operated as a military shooting range, to observe growth. As a result, as shown in Fig. 3, it was confirmed that the gallop grows well near the pullout area without artificial management. In order to confirm the applicability to the field, it was observed whether or not the seedlings were grown in the vicinity of the pit area of Gyeonggi-do, which is currently operated as a military shooting range, as shown in Fig. 4 . As a result, as shown in FIG. 5, it was confirmed that the galloping grew well without man-made maintenance near the pull-out area. In other words, the gall bladder and the vermiculata are adaptable to the domestic soil and climate and are capable of growing in any area, and particularly applicable to the field contaminated with the explosive substance, which satisfies the above-mentioned conformity condition (i) .

Regarding the above-mentioned compliance condition (ii), the plant 40 for purifying the explosive substance is economical for a perennial plant that can survive for a long time in one plant. In the late spring and early autumn, the grasses and marsh grasses grew at the end of September and the seedlings grew at the end of September, while the grasshoppers and mullet grew in April, early spring, and faded at the end of October. It is a fast perennial plant. Therefore, gallops and cormorants are able to remove gunpowder substances for a longer period of time than goosebumps and velvet. Especially, in the field such as shooting range contaminated with explosives, there is a risk that the worker 's life may be seriously harmed due to the unexplainable explosion in the shooting training. Therefore, the explosive exploration must be carried out before the plant material. However, perennial plants such as Euphorbiaceae and Lepidoptera, for example, have a burden of exploiting the unexploded ordnance of planting each year, seriously undermining economic efficiency, one of the greatest advantages of flora purification technology. On the contrary, gallops and gabbros are perennial plants, which are cost-effective as compared to annual plants, since only one exploration is required for early planting. In other words, Gallup and Mulberry are perennial plants that grow for a long period of time and are high in economic efficiency, and thus satisfy the above-mentioned condition (ii).

On the other hand, in order for the explosive substance purifying plant 40 to substantially purify the explosive substance, the above-mentioned conditions (iii) to (iv) must be satisfied. Soil port experiments were carried out to confirm the purification ability of gunpowder and crustacean powder. At the same time, mass balance and removal rate of pollutants were calculated.

First, the soil port experiment will be described in detail for the gallop, but it should be understood that the present invention is not limited to the following embodiments.

The soil pot pot experiment of Gallup

Since the solubility of the explosive substance in water is relatively low, acetone, which is highly soluble, is used as an artificial pollution solvent. TNT or RDX was dissolved in acetone, sprayed evenly on un-contaminated soil, mixed and removed by volatilization of acetone in a cool, shady place. The addition of 30% of mulberry japonica to artificially contaminated soils was uniformly mixed to ensure permeability and permeability necessary for plant growth. 2 kg of powdered artificial soil material was contained in the pot reaction tank, and the oiled plants germinated on the plant mat were planted and cultivated in a greenhouse (30 ° C) for 134 days. All experiments were carried out in quadruplicate, and control was also run in the control plot planted in non - contaminated soil. After the cultivation was completed, the biomass and dry weight of the Galilei were counted. The results are shown in FIG. 6, and the length growth (ground height) of the Galilei within the growing period was measured and the results are shown in FIG.

As shown in FIG. 6, the growth and dry weight of the gall bladder planted in the soil contaminated with explosive substance-contaminated soil were significantly lower than those of the non-contaminated soil. Biomass above the control was 12.2% for TNT treatment and 26.1% for RDX treatment. The surface dry weight was 15.6% for TNT treatment and 28.4% for RDX treatment compared to the control. In addition, as shown in Fig. 7, the ground level of 100 days of cultivation was 65.6% for TNT treatment and 72.7% for RDX treatment compared to the control, and there was also a difference in plant growth.

After the cultivation in the pot was completed, the explosive substance in the soil was extracted by the same method as SW-846 method 8330B developed by the US Environmental Protection Agency to analyze the explosive substance in soil and plant body. And analyzed by HPLC. In addition, the plant explosive materials were extracted and analyzed in the same manner as above, and the concentrations of the powdery materials in the soil / plant of TNT and RDX treated by gallstones are shown in FIG.

As shown in FIG. 8, the initial TNT concentration of the contaminated soil was 207.4 mg / kg, but the final concentration was 3.75 mg / kg, and the initial RDX concentration decreased from 290.9 mg / kg to 202.8 mg / kg . The concentrations of TNT were not detected in the upper part of the plant, and 29.3 mg-TNT / kg was detected in the lower part of the plant. The concentrations of 2A-DNT and 4A-DNT in TNT were 50.48 mg / kg and 38.4 mg / It was confirmed that this TNT was absorbed and converted. In the RDX treated area, 1,161.2 mg / kg was detected in the upper part of the gallbladder, and 80.0 mg / kg was detected in the lower part, and it was confirmed that more explosive substance was transferred to the ground part. However, MNX, DNX and TNX, which are the products of conversion of RDX, were not detected at the surface.

The mass balance was calculated based on the analysis results of the above plant and soil explosives. The results are shown in FIG.

As shown in Figure 9, 1.82% of the initial dose remained in the soil at TNT treatment, 0.14% was the 4,4'-azoxy compound, a biological / abiotic conversion product, 0.05% TNT, and 0.14% was present as a conversion product in the ground. In the case of RDX, 68.8% of the initial dose remained in the soil, 0.91% in the ground part and 0.08% in the ground part. Plant root and microbial interactions in soil caused 31.1% to be removed as unknown.

In addition, the toxicological changes caused by gunpowder substances in Soil treated with galloping were analyzed by microtox equivalent method using light emitting microorganisms. Specifically, the soil contaminated with initial and final explosive substances was air-dried and classified into No. 30 sieve to ensure homogeneity of the soil. Then, 5 g of a soil sample and 20 mL of a 2% NaCl solution were added to a 40 mL amber vial, . The mixed sample was stirred with a shaker, the soil particles were settled, the supernatant was filtered with a 0.2 μm PTFE filter, and 0.5 ml of a 2% NaCl solution and a sample to be measured were added to the measurement cuvette. 5 ml of the active solution was added to the freeze-dried fluorescent bacteria and left to be allowed to activate for 15 minutes. After the activation, 0.5 ml of the above-mentioned activated bacteria was added to the measurement cuvette, and the mixture was measured for 15 minutes. The acute toxicity results of the initial and final soil of the planting material are shown in Fig.

As shown in FIG. 10, the initial toxicity of TNT treatment was 74%, but it was greatly reduced to 20% after chlorosis planting, and the inhibition rate of light emission of the RDX treated soil was reduced by 2% from the initial 27% to the final 25%.

As shown above, the domestic native grasshopper, Gallup, was cultivated in soil contaminated with explosive substances, and after 134 days, the initial TNT was 207.4 mg / kg, but the final concentration was decreased to 3.75 mg / kg. Was reduced from 290.9 mg / kg to 202.8 mg / kg. In addition, it was confirmed that 2A-TNT and 4A-DNT, which are TNT conversion products, are detected and can be reduced and eliminated by TNT. As a result of evaluating the acute toxicity to the soil before and after the treatment, the initial inhibition rate of TNT treatment was 74%, but it decreased to 20% after the killer planting, and the inhibition rate of light emission of RDX treated soil was 27% , Respectively.

Therefore, the gallop is not only resistant to the explosive substance in the soil contaminated with the explosive substance but also has excellent ability to absorb, decompose and remove the explosive substance, so that when the gallop is planted in the contaminated soil, Can be removed and then removed to reduce the natural emissions of chemicals and protect the ecosystem around the polluted area. That is, the gall pillels satisfy the above-mentioned conditions (iii) to (iv).

Next, the soil port experiment will be described in detail with respect to the vermiculite. It should be understood that the present invention is not limited to the following embodiments.

Upholstery soil pot experiment

Since the solubility of the explosive substance in water is relatively low, acetone, which is highly soluble, is used as an artificial pollution solvent. TNT or RDX was dissolved in acetone, sprayed evenly on un-contaminated soil, mixed and removed by volatilization of acetone in a cool, shady place. The addition of 30% of mulberry japonica to artificially contaminated soils was uniformly mixed to ensure permeability and permeability necessary for plant growth. 2 kg of powdered artificial soil material was contained in the pot reaction tank, and the oiled plants germinated on the plant mat were planted and cultivated in a greenhouse (30 ° C) for 134 days. All experiments were carried out in quadruplicate, and control was also run in the control plot planted in non - contaminated soil. After the end of cultivation, the biomass and dry weight of the groundwater were measured, and the results are shown in Fig. 11. The length growth (ground height) of the mungbean in the cultivation period was measured and the results are shown in Fig.

As shown in FIG. 11, the individual growth and dry weight of the vermiculite planted in the soil contaminated with explosive materials were significantly lower than those of the non-polluted soil (control). The dry weights of ground and underground were decreased to 29.6% and 35.5% in TNT treatment and 24.6% and 22.5% in RDX treatment, respectively. The top and bottom dry weights of cormorant planted in the control were 2.37g and 5.16g, respectively, and decreased to 0.70g and 1.83g in the TNT plant and 0.58g and 1.16g in the RDX plant, respectively. As described above, the biomass of the ground and underground was decreased due to the extremely high concentration of the explosive substance, but the plant did not die.

After the cultivation in the pot was completed, the explosive substance in the soil was extracted by the same method as SW-846 method 8330B developed by the US Environmental Protection Agency to analyze the explosive substance in soil and plant body. And analyzed by HPLC. In addition, the plant explosive materials were extracted and analyzed in the same manner as above, and the soil / plant explosive substance concentrations of the TNT and RDX treatments due to the germination are shown in FIG.

As shown in FIG. 13, the initial TNT concentration of the contaminated soil was 207.4 mg / kg, but the final concentration was decreased to 12.46 mg / kg, and the initial concentration of RDX treatment decreased from 290.9 mg / kg to 146.69 mg / kg . The concentration of the plant top was not detected in the upper part of the TNT treatment and 61.59 mg-TNT / kg was detected in the lower part. The conversion products of 2A-DNT and 4A-DNT of TNT were detected in 83.72 mg / kg and 71.10 mg / It was confirmed that this TNT was absorbed and converted. In the RDX treatment, 720.0 mg / kg was detected in the upper part of the ground, and 237.17 mg / kg was detected in the lower part, indicating that more RDX was transferred to the upper part of the ground. However, MNX, DNX and TNX, which are the products of conversion of RDX, were not detected at the surface.

The mass balance was calculated based on the analysis results of the above plant and soil explosives, and the results are shown in FIG.

As shown in Fig. 14, 6.04% of the initial dose in the TNT treatment remained in the soil, 0.20% was the 4,4'-azoxy compound, a biological / anabolic conversion product, 0.02% TNT, and 0.08% was present as a conversion product in the ground. In the case of RDX, 49.09% of the initial dose remained in the soil, 0.07% in the ground part and 0.05% in the ground part. 50.78% was removed as undetermined material due to plant root and microbial interactions in the soil.

In addition, toxicological changes caused by gunpowder substances in soils treated with mulberry were analyzed by microtox equivalent method using light emitting microorganisms. Specifically, the soil contaminated with initial and final explosive substances was air-dried and classified into No. 30 sieve to ensure homogeneity of the soil. Then, 5 g of a soil sample and 20 mL of a 2% NaCl solution were added to a 40 mL amber vial, . The mixed sample was stirred with a shaker, the soil particles were settled, the supernatant was filtered with a 0.2 μm PTFE filter, and 0.5 ml of a 2% NaCl solution and a sample to be measured were added to the measurement cuvette. 5 ml of the active solution was added to the lyophilized fluorescent bacteria, allowed to be activated for 15 minutes, and 0.5 ml of the above-mentioned activated bacteria was added to the measurement cuvette after the activation, and the mixture was measured for 15 minutes. The results of acute toxicity of the initial and final soil of the plant material are shown in Fig.

As shown in FIG. 15, the initial toxicity of the TNT treatment was 74%, but it was greatly reduced to 17% after the planting of the wheat, and the inhibition rate of the light emission of the RDX treated soil was reduced by 7% from the initial 27% to the final 20%.

As mentioned above, after cultivating the domesticated domesticated grasshopper, domesticated grassland, in the soil contaminated with explosive materials, the final TNT concentration was 207.4 mg / kg after 134 days, but the final concentration was decreased to 12.5 mg / kg. Decreased from 290.9 mg / kg to 146.7 mg / kg. In addition, it was confirmed that TNT conversion products, 2A-TNT and 4A-DNT, were detected in the underside (root) of the sea urchin, and TNT could be reduced and eliminated. As a result of evaluating the acute toxicity to the soil before and after the treatment, the initial inhibition rate of TNT treatment was 74%, but it decreased to 17% , Respectively.

Therefore, it is not only pollution resistance to explosive materials in the soil contaminated with gunpowder, but also the ability to absorb and decompose gunpowder substances. Therefore, when cormorant is planted in contaminated soil, Can be removed and then removed to reduce the natural emissions of chemicals and protect the ecosystem around the polluted area. Namely, the cormulus satisfies the above conditions (iii) to (iv).

Meanwhile, the method for purifying the flora of the soil contaminated with the explosive substance according to one embodiment of the present invention uses the port 100 for flora purification of the explosive substance according to one embodiment of the present invention. That is, a method for flora purification of a soil contaminated with a powdered substance according to an embodiment of the present invention includes: (1) a body 10 made up of a biodegradable material opening up and down and having a waterproof function; And a cover 30 which is detachably attached to the lower portion of the mesh 20 and which is provided with a cover 30 for passing water and air therethrough and preventing the soil 11 from flowing out. A first step (S10) of planting a potash material purifying plant (40) filling the soil (11) of the body (10) of the pot (100) And a second step (S20) of disposing the port (100) in a zone contaminated with the explosive substance after removing the first port (30). At this time, the first step (S10) may further include a step (S11) of planting Galp or cormorant as the flora.

Since the body 10, the mesh 20, the cover 30, and the plant 40 for purifying the explosive substance are the same as those described in the port 100 for the flushing agent of the explosive substance according to one embodiment of the present invention, Is omitted.

In order to confirm the applicability of the present invention to the field, a gallop was planted in the ground-type port 100 for flora purification according to an embodiment of the present invention, and as shown in FIG. 16, For about 3 weeks. Thereafter, as shown in Fig. 17, the ground surface type port 100 for flora purging according to an embodiment of the present invention in which the gallows are planted is placed on the A shots field pile without any other treatment Respectively. At this time, the cover 30 made of a nonwoven fabric was removed before being placed on a piece of land. The ground-type port 100 for flora purging according to an embodiment of the present invention in which the gallows are planted is arranged in the fall in order to confirm whether the gallows have roots in the field before the winter and normally winter and then grow again the following year. As shown in Fig. 18, when the ground trousers were planted in the ground-type port 100 for flora purification according to an embodiment of the present invention, But it was found that the roots grew and reached the soil in the field through the plastic mesh 20. As a result of the observation again on March 29, 2014, the gallop planted in the ground-type port 100 for flora purification according to one embodiment of the present invention was successfully wintered as shown in Fig. 19, Were found to be firmly adhered to the soil in the field. Then, as a result of the observation again on May 17, 2014, as shown in Fig. 20, the sprouts started to grow on the gallows, and the body 10 made of corrugated cardboard, Respectively. As described above, it has been confirmed that the ground-type port 100 for flora purification according to one embodiment of the present invention planting gallop, an indigenous wild grass native to the country, has excellent field applicability applicable to sites contaminated with explosives materials .

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the exemplary embodiments or constructions. It will be apparent to those skilled in the art that various modifications and equivalent arrangements may be made without departing from the scope of the invention. You will understand the point. Accordingly, the true scope of protection of the present invention should be determined only by the appended claims.

10: Body 11: Clay
20: mesh 30: cover
40: Plants for the purification of explosives substances

Claims (1)

A body which is vertically opened and filled with soil in an inner space and is made of papers coated with a biodegradable oily material having a waterproof function and whose lower portion is formed in a tapered shape wider than an upper portion;
A mesh provided at a lower portion of the body and made of a material having a higher toughness than the body;
A cover made of a nonwoven fabric so as to be detachably attached to the lower portion of the mesh and to prevent water and air from passing therethrough, And
A plant for plant protection purification selected from grasshoppers or sea grasses to be planted in the soil and to absorb and remove the explosive substance in vivo,
Wherein the gunpowder material is TNT (2,4,6-Trinitrotoluene) or RDX (Royal Demolition eXplosive, Hexahydro-1,3,5-trinitro-1,3,5-triazine) Of ground surface for flora purification.

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