KR200450418Y1 - Dish for cell or tissue culture - Google Patents

Abstract

The present invention relates to an apparatus for culturing cells or tissues, and more specifically, a cover glass in which cells or tissues are cultured; Petri dishes for placing the cover glass; And a culture dish cover covering the culture dish, wherein the culture dish has one or more grooves formed at a bottom edge thereof.

In addition, another embodiment includes a cover glass in which cells or tissues are cultured; Petri dishes for placing the cover glass; And a culture dish cover covering the culture dish, wherein the culture dish has one or more protrusions formed on a bottom surface thereof.

Cell or tissue culture instruments, cover glass, immunocytochemistry, immunofluorescence

Description

Dish for cell or tissue culture

The present invention relates to an apparatus for culturing cells or tissues, and more particularly, when a cover glass is put into a cell or tissue culture dish and the cells are cultured according to an experimental method or purpose, the cells are mainly cultured on the cover glass. Thus, when performing experiments related to immunocytochemical studies or immunofluorescent studies with primary and secondary antibodies, the cells proliferate and the cells do not have to be attached to the cover glass without removing them from the cells or tissue culture dishes. Alternatively, by performing immunocytochemical staining or immunofluorescent staining in a tissue culture dish, not only the cover glass in which the cells have been grown can be broken, but also the labor of the experiment can be easily saved, and the labor cost can be reduced. There is also a reduction in the experimental cost of the primary or secondary antibody as a reagent. The present invention relates to a cell or tissue culture dish designed so that the cover glass is not broken by allowing the cover glass to be detached from the cell or culture dish very easily when it is to be observed under a microscope after all staining processes are completed.

Cell or tissue culture technology is a very basic and important technology in biological research, including molecular biology, and researches on cancer diagnostic research, biopharmaceutical research including cancer therapeutic substances, gene therapy research, stem cell differentiation research, and characteristic substance research. It is a very basic and basic technology that is being used in so many different research fields.

Cells cultured by various methods according to the above research purpose may perform functional or live cell research of the cells in the state of living cells, or paraform aldehyde, ethanol, methanol Select cells or tissue fixatives such as methanol and acetone to fix them so that they do not degenerate, and then repeat washing, primary antibody reaction, repeated washing, secondary antibody reaction, repeated washing, color reaction, repeated washing, etc. The experiment was performed by completing and encapsulating the immunocytochemical staining or immunofluorescence staining in the process of observing with a microscope suitable for the purpose of experiments such as a light microscope, a fluorescent microscope, and a confocal laser microscope. Results can be quantified, and confidence in the reproducibility of the experiment can be secured.

In this regard, cell culture methods for immunocytochemical or immunofluorescence studies can be largely divided into two methods.

First, after culturing cells in a circular cell or tissue culture dish widely used for cell culture, the cultured cells are treated with trypsin or the like to separate the cells from the cells or tissue culture dish and collect them for histological examination. Attach to glass slide. The cells attached to the histological glass slides are fixed so as not to be denatured, and an antibody suitable for the purpose of the study is selected and encapsulated in a cover glass through the above immunocytostaining or immunofluorescent staining. It is a method to observe the enclosed sample under a microscope suitable for the purpose of research. However, this method is difficult to attach evenly to monolayers, such as cells or cells cultured in a tissue culture dish and proliferated when the cultured cells are attached to the histological glass slide, and in particular, the cells collected from the cells or tissue culture dishes It should be fixed immediately after being placed on the bioslide glass slide to minimize cell degeneration.

However, in this process, the adhesion of the cells is insufficient, there was a problem that many cells are eliminated. It may also be attempted by coating a material that facilitates cell adhesion to the biopsy glass slide, but even though the glass slide for biopsy is coated, a certain amount of time is required for the cells to adhere. Errors in cell-related experimental results were inevitable. Therefore, this method was not used for immunocytochemical studies or immunofluorescence studies, but was limited to only simple forms observation.

The second method compensates for the problems related to the number of adherent cells or the possibility of denaturation. The cover glass, which is a support to which cells can be attached at all, is grown from poly-L-lysin or gelatin. (gelatin) and then cultured the cells in a conventional cell or tissue culture dish of widely used cells, and when the cell culture is complete, remove the cover glass from the cell or tissue culture dish to immunocytochemical staining or immunofluorescence How to proceed dyeing.

For reference, the dictionary meaning of the cover glass is 'the cover glass of the slide specimen, the protective glass of the projection film slide', and the use in biology or medicine is attached to the histological glass slide microscope It is a very thin transparent glass around 0.1 mm, covering the tissue specimen with encapsulant before observation.

The method using such a cover glass will be described in detail with reference to the accompanying drawings.

First, FIG. 1 is a view showing a cover glass in a cell or tissue culture apparatus according to the prior art.

Referring to FIG. 1, a cell or tissue culture apparatus 10 according to the prior art includes a culture plate 12 and a cover 11 that covers the culture plate 12. At this time, when the cells and the culture medium is put in the culture dish 12 and the cells are cultured according to the culture conditions, the cells proliferate on the inner surface of the bottom of the culture dish 12, to observe the state of proliferation of the cells with an inverted microscope (inverted microscope) In order to have good light transmission, the culture dish 12 and the lid 11 are very transparent, and the thickness of the bottom of the culture dish 12 is generally about 1 mm.

For immunocytochemical or immunofluorescence studies, a cover glass 13 serving as a support for attaching cells to the culture dish 12 is cultured and the cells are cultured according to the culture conditions. The cells adhere to the inner surface of the bottom and proliferate, but the cells also proliferate on the upper surface of the cover glass 13.

When the cell proliferation is completed, the cover 11 is opened, the cover glass 13 is taken out with tweezers, the cells attached to the cover glass 13 are fixed, and the immunocytological staining using a specific antibody ( An immunocytochemical stain or immunofluorescent stain is performed.

Immunohistochemical staining and immunofluorescence staining differ in content, but there is a similarity in the overall process of the experimental procedure. In other words, fixation to prevent denaturation of cells, multiple repeated washings, primary antibody application, constant time reaction, multiple repeated washings, secondary antibody application, constant time reaction, multiple repeated washings, colorant application, constant time reaction After repeated washings and attaching the cover glass, which has been dyed several times, to the biopsy glass slide with the sealant (encapsulation opposite to the normal seal), the general optical microscope, the fluorescence microscope, and the confocal laser Select and observe a microscope suitable for the purpose of the research, such as a microscope.

However, in the case of culturing the cells by putting the cover glass 13 in the culture dish 12 according to the prior art described above, there was no culture dish 12 similar to the size or shape of the cover glass 13, so that the cover The conventional circular culture dish 12 larger than the glass 13 was used.

In this case, however, immunocytochemical staining or immunofluorescence staining may be performed without leaving the cover glass 13 in the culture dish 12, but primary and primary reagents for immunocytochemical staining or immunofluorescence staining may be used. Expensive experimental reagents such as secondary antibodies and color reagents were wasted. In order to reduce these expensive reagents, the cover glass 13, which is very thin to 0.1 mm, is taken out with tweezers or the like and subjected to the above immunocytochemical staining or immunofluorescence staining, which is composed of reactions and washing processes. There was no.

In addition, as described above, after the cell culture is performed by the cell culture method according to the related art, the cover glass 13 is used by using tweezers or the like to proceed with the experiment process of the next step. The cover glass 13 is very thin (0.1 mm), and the cell culture fluid penetrates between the cover glass 13 and the culture dish 12 so that a membrane is formed. It was very difficult to separate the cover glass 13 from the culture dish 12 using a similar mechanism.

 In particular, since the cover glass 13 is made of glass, it is easy to be damaged by tweezers or the like in the separating process.

In addition, after the cover glass 13 is removed from the culture dish 12 in order to proceed with the experiment process of the next step after proliferating the cells on the cover glass 13 by the cell culture method according to the prior art, Since the process of picking up and staining the cover glass 13 with tweezers or the like is long and repeated, not only does it require a lot of labor, but also the cover glass 13 is easily broken in several repeated reactions or washing processes. In many cases, the thin and transparent cover glass 13 had chaos in the cell-attached and unattached surfaces.

Such research related to cell culture using the conventional cover glass 13 has been continued for several decades, but the technology for solving the above problems has not been developed, and of course, the product has not been realized.

Therefore, as a method of supplementing the disadvantages of the prior art of culturing cells by placing the cover glass 13 in a general culture dish 12, 1) the cover glass to the culture dish in order to facilitate the experiment process after cell culture By carrying out the immunocytochemical or immunofluorescence staining without leaving it out, it is possible to reduce the reagent cost of expensive primary or secondary antibodies, and 2) hold the cover glass with tweezers or similar instruments and take several steps. It saves the labor of carrying out the configured immunocytochemical staining or immunofluorescence staining, and 3) the thin cover glass does not break during several stages of the experiment, and 4) the cover glass is eventually taken out at the end of the experiment. At this time, the cover glass and the cell that can easily separate the culture dish or Development of tissue culture apparatus was urgently needed.

The present invention was devised to solve the above-mentioned problems, and a first object of the present invention is to provide a cell or tissue culture apparatus that can reduce the reagent cost of expensive primary or secondary antibodies in cell or tissue culture.

In addition, the immunocytochemical staining or immunofluorescence staining process after the cell or tissue culture according to the prior art can be easily performed in the cell or tissue culture dish without removing the cover glass, thereby reducing labor and research time, and improving the efficiency of experiments. Another object is to provide a tissue culture apparatus.

A third object is to provide a cell or tissue culture apparatus capable of preventing breakage of a cover glass made of very thin glass.

In addition, a fourth object of the present invention is to provide a cell or tissue culture apparatus that can easily and easily separate the cover glass from the culture dish.

The present invention for achieving the above object, the cover glass cultured cells or tissues; Petri dishes for placing the cover glass; And a culture dish cover covering the culture dish, wherein the culture dish has one or more grooves formed at a bottom edge thereof.

Preferably, one side or both sides of the groove is formed with a protruding pillar for preventing the cover glass from covering the groove.

Preferably, the culture dish has a side portion protruding outward to form an extension space.

Preferably, the groove is formed on the extension space, and extends toward the inner bottom surface of the culture dish to form a gap in the bottom surface of the cover glass.

On the other hand, as another means for achieving the above object, a cover glass cultured cells or tissues; Petri dishes for placing the cover glass; And a culture dish cover covering the culture dish, wherein the culture dish has one or more protrusions formed on a bottom surface thereof.

Preferably, the cover glass, the culture dish and the culture dish cover all have the same shape.

The present invention has the following excellent effects.

First, for the experimental process after cell culture, the immunocytochemical staining or immunofluorescence staining can be performed without leaving the cover glass in the culture dish. By simplifying the procedure of holding the process with tweezers, the efficiency of the experiment can be increased by reducing labor and research time.

In addition, it is possible to reduce the cost of reagents such as expensive primary or secondary antibodies by minimizing unnecessary internal areas irrelevant to cell or tissue culture.

In addition, it is possible to prevent the cover glass made of very thin glass from being broken during the multi-step experimental process.

In addition, there is an excellent effect that can easily and easily separate the cover glass from the culture dish.

The terminology used in the present invention is a general term that is widely used at present. However, in some cases, the term is arbitrarily selected by the applicant. In this case, the meaning used or described in the detailed contents for carrying out the present invention is not merely a name of the term. Considering this, the meaning should be grasped.

Hereinafter, with reference to the preferred embodiments shown in the accompanying drawings will be described in detail the technical configuration of the present invention.

First, Figure 2 is an overall configuration of the cell or tissue culture apparatus according to the first embodiment of the present invention.

Referring to Figure 2 (a), the cell or tissue culture apparatus according to the first embodiment of the present invention consists of a cover glass 110, a culture dish 120 and a culture dish cover 130.

The cover glass 110 is a part in which cells or tissues are cultured. After the cell or tissue is cultured, the cover glass 110 is placed on a glass slide for biopsy, attached to a tissue sample, stained, and then encapsulated.

At this time, the glass slide is a transparent rectangle having a thickness of about 1 mm, although the size is not compulsorily standardized, but standardized naturally since the biopsy of the hospital for pathological diagnosis of a patient's disease has been conducted. It is.

Such standardized glass slides have an international trend of about 26 mm in length and about 75 mm in width, and the standardized glass slides are generally used in biological research.

Therefore, in embodiments of the present invention, the cover glass 110 may be made of various sizes, shapes and materials that can be attached to the glass slide.

However, in a preferred embodiment of the present invention, the cover glass 110 is made of a transparent glass plate of a square of 0.1 mm in thickness, the length of the horizontal and vertical up to 24 mm.

This means that when culturing cells or tissue in the cover glass 110 having the size, the cover glass 110 can be attached to the glass slide for one histological examination at the same time, so that the experiment time can be shortened and the experiment through the same. This is because the efficiency can be maximized.

On the other hand, the culture dish 120 is a portion where the cover glass 110 described above, one or more grooves 140 are formed on the bottom edge or corner of the culture dish 120.

In a preferred embodiment of the present invention the groove 140 is provided in one place on the side edge of the culture dish 120.

On the other hand, the groove 140 serves to prevent a portion of the cover glass 110 is in close contact with the bottom surface of the culture dish 120.

Through this, in the step of separating the cover glass 110 into the culture dish 110, it can be easily and easily separated using tweezers or the like.

The culture dish 120 may be formed in various sizes, materials, and shapes in which a space in which the cover glass 110 may be placed is formed.

However, in the preferred embodiment of the present invention, the culture dish 120 is made of a square having a maximum length of 28 mm in length and width.

In addition, the cover glass 110 and the culture dish 120 to maintain the difference between the horizontal and vertical length within 5mm, which is unnecessary space in the bottom surface of the culture dish 120 (the culture dish 120 This is to prevent waste of expensive experimental reagents by minimizing the remaining space of the bottom surface of the bottom surface except for the portion where the cover glass 110 is placed.

On the other hand, the culture dish cover 130 is a portion covering the culture dish 120, it is made of the same material as the culture dish 120 can still check the culture state of the cells or tissues.

On the other hand, Figure 2 (b) is a view showing another embodiment of the present invention, the cover glass 110, the culture dish 120 and the culture dish cover 130 is a cell or tissue culture apparatus made of a circular Since the description of the components has been described fully with reference to FIG. 2 (a), it will be omitted below.

Hereinafter, with reference to Figure 3 which is the overall configuration of the cell or tissue culture apparatus according to a second embodiment of the present invention.

Referring to Figure 3 (a), the cell or tissue culture apparatus according to the second embodiment of the present invention is the same as the first embodiment cover glass 110, the culture dish 120 and the culture dish cover 130 ), And the culture dish 120 has one or more grooves 140 formed at the bottom edge thereof.

At this time, one side or both sides of the groove 140 may be formed with a protruding pillar 150, in a second preferred embodiment of the present invention, the protruding pillar 150 is on both sides of the groove 140 Formed.

The reason for forming the protrusive pillar 150 as described above is that the cover glass 110 is moved by an external force including a vibration applied from the outside of the culture dish 120 to block the groove 140. This is to prevent.

Through this, even if the cover glass 110 by any movement of the cover glass 110 can not cover the groove 140 as a whole, separating the cover glass 110 from the culture dish 120 Clearances can be secured in which tweezers or similar devices can be inserted.

On the other hand, Figure 3 (b) is a view showing a second embodiment made of a circular rather than square, all components except for the protruding pillar 150 will be the same as described above in the first embodiment will be omitted below Do it.

Hereinafter, with reference to Figure 4 which is the overall configuration diagram of a cell or tissue culture apparatus according to a third embodiment of the present invention.

Referring to Figure 4 (a), the cell or tissue culture apparatus according to the fourth embodiment of the present invention is the same as the first embodiment described above cover glass 110, the culture dish 120 and the culture dish cover ( 130, wherein at least one groove 140 is formed at the bottom edge of the culture dish 120.

On the other hand, the culture dish 120, as shown in Figure 4 (a) is a portion of the side protrudes in the outward direction is formed an extension space 160, the groove 140 is on the extension space 160 Is formed in, but extends toward the inner bottom surface of the culture dish 120 to form a gap in the lower surface of the cover glass (110).

The extension space 160 is configured to perform the same role as the protrusive pillar 150 according to the second embodiment, regardless of the movement of the cover glass 110 on the lower surface of the cover glass 110. It forms a gap into which tweezers or similar instruments can be inserted.

And, Figure 4 (b) is a view showing a third embodiment made of a circular rather than square, all components except for the overall shape will be the same as described through Figure 4 (a) will be omitted below. .

Hereinafter, with reference to Figure 5 which is the overall configuration of the cell or tissue culture apparatus according to a fourth embodiment of the present invention.

Referring to Figure 5 (a), a cell or tissue culture apparatus according to a fourth embodiment of the present invention includes a cover glass 110, a culture dish 120 and a culture dish cover 130 and the culture One or more protrusions 170 are formed on the bottom surface of the dish 120.

The protrusion 170 serves to prevent the cover glass 110 from being in close contact with the bottom surface of the culture dish 120.

In this way, in removing the cover glass 110, a gap in which a tweezers or other similar mechanism can be inserted is secured, so that the cover glass 110 can be easily and easily separated from the culture dish 120. .

At this time, the protrusion 170 may be formed in a plurality of positions and heights in a plurality, in the fourth preferred embodiment of the present invention is formed to maintain a maximum height of 1 mm in four places on the edge of the culture dish 120 It was.

Hereinafter, the cover glass 110, the culture dish 120, and the culture dish cover 130 are the same as described through the first embodiment, and thus will be omitted below.

On the other hand, Figure 5 (b) is a circular shape unlike the overall shape of Figure 5 (a), except for this all components are the same as described through Figure 5 (a) will be omitted below.

As a result, the immunocytochemical staining or immunofluorescence staining was performed with the cover glass intact without taking out of the culture dish for the experimental procedure after the cell culture through the first to fourth embodiments of the present invention described above. In this way, the efficiency of the experiment can be improved by reducing labor and experiment time, and the unnecessary cost of the culture dish can be minimized to prevent waste of expensive primary or secondary antibodies, thereby reducing reagent costs.

In addition, it is possible to prevent the cover glass made of very thin glass from being broken during the multi-step experimental process.

In addition, there is an excellent effect that can easily and easily separate the cover glass from the culture dish.

As described above, the present invention has been illustrated and described with reference to preferred embodiments, but is not limited to the above-described embodiments, and is provided to those skilled in the art without departing from the spirit of the present invention. Various changes and modifications are possible by this.

1 is a view showing a cover glass in a cell or tissue culture apparatus according to the prior art.

2 is an overall configuration diagram of a cell or tissue culture apparatus according to the first embodiment of the present invention.

3 is an overall configuration diagram of a cell or tissue culture apparatus according to a second embodiment of the present invention.

4 is an overall configuration diagram of a cell or tissue culture apparatus according to a third embodiment of the present invention.

5 is an overall configuration diagram of a cell or tissue culture apparatus according to a fourth embodiment of the present invention.

<Description of the symbols for the main parts of the drawings>

110: Glass cover 120: Culture plate 130: Culture plate cover

140: groove 150: protrusion pillar 160: extension space 170: protrusion

Claims (6)

In a cell or tissue culture apparatus, A cover glass in which a thickness of 0.1 mm to 0.2 mm and a horizontal length of 10 mm to 24 mm are square, and the cells or tissues are cultured; A culture dish in which the cover glass is placed and a portion of the side surface protrudes outward to form an extension space; And And a culture dish cover covering the culture dish, wherein the bottom surface of the extension space includes a groove having a predetermined shape. The method of claim 1, The groove is formed on the extension space, the cell or tissue culture apparatus, characterized in that extending to the inner bottom surface of the culture dish to form a gap in the lower surface of the cover glass. The method of claim 2, The cover glass, the culture dish and the culture dish cover are all cells or tissue culture apparatus, characterized in that the same shape. delete delete delete

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