KR20040108476A - Making methods of effectively bio-transformed ginsengs using the cultivation of edible and medicinal mushrooms - Google Patents

Abstract

PURPOSE: A method of making a ginseng product by soaking cut pieces of ginseng in the yeast extract, inoculating them with mushroom strains such as Paecilomyces tenuipes, Cordyceps militaris, Cordyceps sinensis and Phellinus Linteus and then culturing them is provided. The product has the increased content of ginsenoside and beta-glucan as well as has enhanced antioxidant effect, antineoplastic effect, antifatigue effect and skin whitening effect. CONSTITUTION: Ginseng is cut into a predetermined size, soaked in 0.1 to 10% of the yeast extract for 2 to 24hr and sterilized at 121deg.C at 1.5atm for 15 to 60min. Mushroom strains such as Paecilomyces tenuipes(KCTC 18063P), Cordyceps militaris(KCTC 6064), Cordyceps sinensis(KCTC 16137) and Phellinus Linteus(KCTC 10055BP) are inoculated into the culture medium and then cultured at 15 to 35deg.C at 100 to 1,000lux for 1 to 4 weeks.

Description

Making methods of effectively bio-transformed ginsengs using the cultivation of edible and medicinal mushrooms

The present invention relates to a method for producing a high functional, high value-added original ginseng product using the biotransformation method of ginseng through edible and medicinal mushroom culture.

Conventional processed ginseng is made from primary processed ginseng (white and red ginseng) and fresh ginseng or primary processed ginseng which are processed by drying as a basic means while maintaining the original shape of Korean ginseng. It can be divided into secondary processed products processed by. However, since the second processed product also uses the first processed ginseng, it starts with two types of basic raw materials: white ginseng and red ginseng. The basic raw material is that the processing method is simply heat treatment.

The research that has been limited to the cultivation and simple processing of ginseng, and the function and formulation of red ginseng has not only caused the export factor of white ginseng to deteriorate the export of white ginseng but also simplifies the processing method. It is true that it has been an obstacle to making world-class variety of Korean ginseng products. Recently, new processed products of Korean ginseng have been released, but these technologies have not been able to change the new functional paradigm that is synergistic with the advantages of two or more raw materials.

In the study of ginseng using microorganisms in relation to the present invention, Korean paper Kim, Sang-Dal (Kim, Sang-Dal. 1989. Production conditions of ginsenoside Rb1 converting enzyme by Rhizopus japonicus . Journal of the Korean Society of Bacteria. 17 (1): 31-34) (Kim Sang-dal, Seo Jung-hoon. 1982. Conversion of ginseng saponin by enzymes produced by Rhizopus sp. Kor. J. Appl. Microbiol. Bioeng . 10 (4) 267-273), but this is done by using a fungus called Rhizopus . It is a study on the conversion enzyme of Rb1 in the side and it is a strain that is completely different from the microorganisms ( Paecilomyces tenuipes, Cordyceps militaris, Cordyceps sinensis, Phellinus linteus ) to be used in the present invention. Can't see

The technology related to the culture of ginseng medium of microorganisms has a manufacturing method of ginseng saponin metabolite of Korean Patent Registration No. 0178863, but because the strains used are different from the present invention and the metabolites produced by microorganisms are also different, cordyceps and situation mushrooms are used. It is completely different from the present invention.

Conventional phosphoric acid processing technology is simply a heat treatment method. Existing red ginseng manufacturing method is produced through cumbersome heat treatment method that repeats 'Ginseng (steaming ginseng with steam) and cooling' nine times, and it is very troublesome and expensive because it goes through 9 times of heat treatment process. This happens. In addition, the processing method is a simple method that relies on heat treatment, so it has been an obstacle to the production of various world-class Korean ginseng products. Therefore, high functional and high value-added products should be produced by diversifying the processing method and researching functionalities of ginseng. In other words, it is necessary to develop a product designed in anticipation of specific physiological activity using pure ingredients, and for this, development of Korean ginseng processing method using bioconversion method is necessary.

Bio-converted ginseng produced through the present invention exhibits the efficacy of single and doubled unique bioactive substances such as ginsenosides and betaglucans. Therefore, an object of the present invention is to create a high functional, high value-added original ginseng product by using the biotransformation method of ginseng through edible and medicinal mushroom culture.

1 is a front view of ginseng cultured Snow Cordyceps sinensis

Figure 2 TLC development diagram of ginsenoside analysis of biotransformation ginseng

Control: Rb, Rd, Re and Rg from bottom up

G: Ginseng

PT-G: Snow Transformed Ginseng

CS-G: Synensis Cordyceps Sinensis Ginseng

The strain used in the present invention was used by subcultured with Paecilomyces tenuipes KCTC 18063P, chrysalis Cordyceps sinensis ( Cordyceps militaris KCTC 6064), Synyces cordyceps sinensis KCTC 26137 and Pellinus linteus KCTC 10055BP. Ginseng used Grade 1 dry ginseng sold by the National Agricultural Cooperative Federation.

The present invention is explained in more detail with reference to the following examples. This embodiment shows an example of the present invention and does not limit the technical scope of the present invention.

Example 1

Preparation of Biotransformed Ginseng Using Cordyceps Sinensis and Sacred Mushroom Culture

The dried ginseng raw material is cut into appropriate sizes, and a solution for adding a microbial culture such as yeast extract and soyton (0.1-10%) is prepared. The additive solution is sufficiently absorbed into Korean ginseng for 2-24 hours and then sterilized. Inoculate (0.1-20%) strains of Snow Cordyceps, Pupa Cordyceps, Sinensis cordyceps, and S. mushroom strains prepared by cultivating Korean ginseng prepared by sterilization. Inoculated Korean ginseng is incubated for about 1 week at an appropriate temperature (15-35 degrees) to grow mycelia. After further incubation for 1-2 weeks in the set environment (light at a low temperature of 5 degrees and 100-1,000 lux), the expression of physiological characteristics according to the strains is confirmed. In the case of Cordyceps sinensis, the orange pigmentation and the fruiting body are produced, and in the case of the situation mushroom, the yellowish pigment production is confirmed). Hot-air dried at a temperature of 50-105 degrees to produce Cordyceps sinensis and S. mushroom ginseng.

1 shows an example of a live ginseng Cordyceps sinensis ginseng.

Example 2

Preparation of Hydrothermal and Solvent Extracts of Raw Ginseng

Hot water and solvent extracts were prepared from each of the bio-converted ginseng for the functional food and pharmacological efficacy assay. In the case of hot water extracts, each of the raw conversion ginseng is placed in a heating circulation extractor, added to water three times the weight (v / w), extracted three times at about 100 degrees for 2-6 hours, filtered, and lyophilized. The final extract was obtained. In the case of the solvent extract, each of the live-converted ginseng was put in a heating circulation extraction tank, and 50-70% ethanol or methanol was added three times the weight (v / w) and extracted three times at 75-85 ° C. for 2-6 hours. Filtration, concentration under reduced pressure, and lyophilization gave a final extract.

Example 3

Analysis of Ginsenosides of Biotransformed Ginseng by TLC

Extracts extracted by solvent extraction of fresh ginseng were fractionated through organic solvent fractions according to the polarity, and then each unique component was compared and confirmed by standard TLC analysis of each fraction. The developing solvent and analysis method were used in the method of the paper (Kwak, Sung-Sung, Mi-Ju Kim, Eun-Hee Kim, Young-Ae Kim, 1997. Rapid extraction of ginseng saponin mixture.Kor. J. Food.Sci. The result is shown in FIG.

Newly formed brown spots in the upper part of G-Re have been identified in the Ginseng Cordyceps and Ginseng mutant ginseng, and Ginsenosides, which are assumed to be G-Rd, have been amplified.

Example 4

Analysis of Immune Activity Beta-glucans of Biotransformed Ginseng by Ethanol Precipitation

Ethanol precipitation was used to determine the content of beta glucan, a representative immune-enhancing polysaccharide of Cordyceps sinensis, S. mushroom and Ginseng. For detailed analytical methods, see the method of the paper (Sim, JS, HR Chang, EG Min, YH Kim and YH Han. 2001. Cytotoxicity of Paecilomyces tenuipes against human carcinoma cells, HepG2 and MCF-7 in vitro.Mycobiol . 29 (3) 170 -172) was used, and the results are shown in Table 1.

In the case of snow ginseng and herbaceous live ginseng, the content of beta glucan, a representative immune-enhancing polysaccharide, was about 44% higher than that of the control ginseng.

Table 1

Beta-Glucan Contents of Raw Ginseng and Ginseng Cordyceps Sinensis

Sample Beta Glucan Content (%) Control group (Ginseng) 13.2 Snow Cordyceps Sinensis Transforming Ginseng 19.0

Example 5

Antioxidant Measurement of Biotransformed Ginseng Using Riboflavin Oxidation

In order to measure the antioxidant power of biotransformed ginseng, we used the method of the paper to measure the free radical scavenging effect generated by the oxidation of riboflavin by light. The results are shown in Table 2 (Winterborn, CC, RE Hawkins, M. Brian, and RW Carrell. 1975. The estimation of red cell superoxide dismutase activity.J. Lab. Clin. Med . 85: 337.

When comparing the antioxidant effects of 1% hydrothermal extracts with riboflavin, the antioxidant powers of 14% and 60% increased, respectively, compared to those of the control group Dried Ginseng and Red Ginseng.

TABLE 2

Determination of Antioxidant Effect of Biotransformed Ginseng Using Riboflavin Oxidation

Sample (1% hydrothermal extract) Antioxidative effect(%) Control group (Ginseng) 85.1 Control group (red ginseng) 60.2 Snow Cordyceps Sinensis Live Ginseng 96.7

Example 6

Antitumor Effect of Fresh Ginseng Using MTT Method

In order to measure the anti-tumor effect of biotransformed ginseng, the MTT method, which measures the concentration of MTT-formazan reacting with cancer cells, was measured using the method of the paper. The results are shown in Table 3 (Sim, JS, HR Chang, EG Min, YH Kim and YH Han. 2001. Cytotoxicity of Paecilomyces tenuipes against human carcinoma cells, HepG2 and MCF-7 in vitro.Mycobiol . 29 (3) 170-172.)

Compared to ginseng and red acid used as control, ginseng Cordyceps sinensis-transformed ginseng showed superior antitumor activity against HeLa cancer cell line.

Table 3

Antitumor Effect of Fresh Ginseng Using MTT Method

Sample (hot water extract 25 mg / ml) Tumor cell survival rate (HeLa cell,%) Control group (Ginseng) 75.9 Control group (red ginseng) 79.4 Snow Cordyceps Sinensis Transforming Ginseng 60.0

Example 7

Anti-fatigue Effect of Raw Ginseng

Anti-fatigue effect of freshly transformed ginseng was examined by forced swimming test using ICR crab 8-week-old mice in the tank (Sim Jin Young et al., 2000. Drug Activity of Snow Cordyceps. Kor. J. Pharmacogn . .) Was used, and the results are shown in Table 4.

In the case of the lively transformed ginseng of the Cordyceps sinensis, anti-fatigue effect of more than about 60-70% was observed when compared with the control group of the Snow Cordyceps sinensis alone, which was 2.6 times higher than the control group.

Table 4

Anti-fatigue effect of freshly transformed ginseng using mouse swimming ability evaluation

Sample % Swimming time relative to control Control group (no administration) 100 Dried ginseng administration group 128 Red ginseng group 146 Snowmobile Cordyceps Biotransformation Ginseng Administration Group 259

Example 8

Whitening Effect of Raw Ginseng

In order to measure the whitening effect of fresh ginseng, the whitening effect was measured by inhibiting the activity of tyrosinase, an enzyme involved in melanin pigment production, and the results are shown in Table 5. (Sim, Mi-hee. 2001. Culture and production of wild ginseng A study on its application, Korean Society of Cosmetic Products, 27 (2) 45-56.)

Experimental results showed that synensis Cordyceps sinensis ginseng showed a superior inhibitory effect on tyrosinase activity compared to ginseng and red ginseng.

Table 5

Inhibition of Tyrosinase Activity of Bio-Converted Ginseng Based on DOPA

Sample Anti tyrosinase effect (%) Dried ginseng administration group 64.8 Red ginseng group 51.8 Sinensis Cordyceps Sinensis Ginseng Administration Group 82.3

Example 9

Manufacture of beverages

Add 0.01-0.5% (w / v) of Snowmobile Cordyceps Sinensis, Pupa Cordyceps Sinensis, Sinensis Cordyceps Sinensis, and Green Mushroom Ginseng Extract, respectively, mix 15% Jujube Extract, 7% Angelica Extract and 7.4% Liquid Fructose, and add purified water. After mixing, filling into a drink bottle or pouch, sterilization and cooling to room temperature to prepare a beverage.

Example 10

Preparation of Herbal Medicines

Add 0.01 to 30% of supplementary ginseng extracts from Snowflower Cordyceps, Pupa Cordyceps Sinensis, Sinensis Cordyceps Sinensis and Situary Mushrooms to supplement nutritional supplements (Vitamin, Acetate, Niacinamide, etc.), oligosaccharides, and 50% alcohol to 5-10% of the composition. And mixed. 10% of sterile purified water was added to the mixture and mixed to form granules having a diameter of 1-2 mm. The molded granules were dried and then passed through a 12-14 mesh to uniformly prepare granules. The granules prepared above may be extruded by appropriate amounts into tablets or powders, or filled into hard capsules to produce hard capsules.

As described above, the at least one hydrothermal extract or solvent extract selected from Snow Cordyceps Sinensis, Chrysalis Cordyceps Sinensis, Sinensis Cordyceps Sinensis, and Situary Mushroom Biotransformation Ginseng may be used to produce ginseng ginsenosides and beta glucans, antioxidant effects, antitumor effects, It has the effect of increasing the antifatigue effect and the whitening effect. Therefore, according to the present invention, by containing the extract in beverages, herbal preparations, etc., it is possible to manufacture a highly functional processed product of increased efficacy compared to conventional ginseng processed products.

Claims (6)

Method of manufacturing functional bio-converted ginseng produced after inoculation and incubation of spawns of edible and medicinal mushrooms such as Snow Cordyceps, Pupa Cordyceps, Sinensis Cordyceps and Situary Mushroom In the preparation of the raw ginseng of claim 1, the step of cutting the dry ginseng raw material to a predetermined size and 0.1-10% yeast extract and Soyton, which is a medium component of the microorganism, is immersed in the dry ginseng raw material to infiltrate the dry ginseng raw Next, the culture method, characterized in that sterilization for 15-60 minutes at 121 degrees, 1.5 atm The bioactive substance according to claim 1, incubated for 1-4 weeks at the incubation temperature of 15-35 degrees after the inoculation, and the growth of hyphae is observed, followed by addition of low temperature (less than 5 degrees at the appropriate temperature) and light of 100-1,000 lux. Culture method characterized by the induction of Functional drink and liquor comprising the bioconversion extract of claim 1 as an active ingredient A pharmaceutical formulation containing the bioconversion extract of claim 1 as an active ingredient and formulated in a pharmaceutically acceptable manner by mixing a pharmaceutically acceptable excipient and adjuvant therein. The formulation according to claim 5, wherein the formulation is a granule or a capsule.