KR20040072193A - Korean mistletoe extract having anti-hypertensive activity and the extraction method of its active substances - Google Patents

Abstract

PURPOSE: An extract of Korean mistletoe having anti-hypertensive activity and a preparation method thereof are provided, thereby preventing and treating hypertension by oral administration of the Korean mistletoe extract, so that it can be added into functional food and medicines. CONSTITUTION: The method for preparing extract of Korean mistletoe having anti-hypertensive activity, HKM-110, comprises the steps of: washing Korean mistletoe with water and cutting it; adding water into Korean mistletoe and boiling the mixture for 2 hours; centrifuging the boiled mixture to separate the supernatant; filtering the supernatant with 0.45 micrometer filter paper; and freeze-drying the filtered solution.

Description

Korean mistletoe extract having anti-hypertensive activity and the extraction method of its active substances}

The present invention relates to an extract for antihypertensive which is extracted from Korean mistletoe by hot water. More specifically, the present invention relates to an antihypertensive extract which is further extracted with an organic solvent after hot water extraction by cutting the Korean mistletoe. In another aspect, the present invention is a pharmaceutical composition for antihypertensive containing Korean mistletoe extract as an active ingredient. The present invention is a functional food or food additive for antihypertensive using Korean mistletoe extract as an active ingredient.

In the modern society, the incidence and confirmation rate of various diseases are greatly improved due to environmental pollution, the increase of the elderly population, and the development of diagnostic technology, and as a result, the severity of the diseases that were considered as inevitable diseases caused by the past There is a great deal of research into the understanding and prevention and treatment of these diseases. Among these diseases, especially the increase in the incidence of adult diseases and degenerative diseases that occur frequently from the mid 40s is very high. In Korea, a single disease has the highest mortality rate (approximately 25%) of cancers from multiple sites, but stroke, hyperlipidemia, hypertension, arteriosclerosis, diabetes, and degenerative arthritis are associated with cardiovascular abnormalities. The back is a serious disease group that follows cancer.

Recently, the interest in the health of individuals has increased rapidly in Korea due to the improvement of economic life. Especially in the management of adult diseases, various health maintenance methods, sports therapy, health foods and supplements, preventive medicines, etc. Many methods for treatment are preferred. However, among the adult diseases, hypertension, arteriosclerosis, heart disease, stroke, and the like, which are representative circulatory diseases, have been developed so far, although many drugs have been developed so far, there is no drug that can expect complete treatment. In particular, cardiovascular diseases often cause complications rather than directly cause death, so the management of these diseases can be a very important factor in maintaining health after the middle age.

Hypertension is a phenomenon in which the pressure in blood vessels (particularly arteries) is high.Blood pressure increases with age and causes primary hypertension (or primary hypertension) of unknown cause, and when the cause can be clearly identified, secondary hypertension ( Or symptomatic hypertension). The cause of hypertension was brain death (44%), cardiac death (23%), and kidney death (5%). In addition, hypertension renal failure and cerebral hemorrhage have recently decreased significantly due to the development of blood pressure lowering agents, but instead atherosclerosis. Complications such as cerebral infarction and myocardial infarction are on the rise. The prognosis of hypertension is influenced by regulatory factors such as blood pressure height, age and sex, and separation period, and risk factors such as hyperlipidemia, diabetes, smoking and obesity, but the presence and severity of complications play a decisive role. Therefore, the prevention of these complications, that is, proper blood pressure lowering therapy for the control of hypertension, is the most preferable method for improving and preventing the prognosis of hypertension.

In general, the causes of hypertension include genetic background, personality, obesity, excessive salt intake, mental stress, smoking, drinking, kidney disease, or other underlying diseases. Less than 10%. Therefore, as a primary preventive measure of hypertension, it is important to be aware of individual risk factors, environmental factors, or promoters and to improve lifestyles such as diet and exercise.

Current treatments for hypertension include angiotensin converting enzyme (ACE) inhibitors, angiotensin antagonists, calcium antagonists, α-adrenoceptor antagonists, hydralazine, minoxidil, sodium nitroprusside, and diuretics and central nervous system agents. However, these drugs have the disadvantage that the blood pressure rises again when the administration is stopped. Therefore, in hypertension, ingestion naturally in the same way as a meal in everyday life, the development of functional foods that have an effective effect on the prevention and treatment can be said to be urgent.

The present inventors have found that the Korean mistletoe extract (HKM-110) exhibits an excellent effect of lowering blood pressure, thereby confirming that the HKM-110 can be used for the prevention and treatment of hypertension, and completed the present invention.

The present invention provides a Korean mistletoe extract and a method of preparing the bulbous sphere having antihypertensive activity, and the present invention can be widely used in general and health foods, and can also be administered as a pharmaceutical composition through oral administration, thereby treating hypertension and Another purpose is to provide raw materials such as health supplements.

1 is a diagram showing the blood pressure lowering effect of oral administration of 30 mg of HKM-110 for 3 days in hypertension rats (SHR).

Figure 2 is a diagram showing the blood pressure lowering effect of oral administration of 10 mg of HKM-110 for 3 days in hypertension rats (SHR).

Figure 3 is a diagram showing the blood pressure-lowering effect of the negative HKM-110 solution in hypertension rats (SHR).

4 is a diagram showing the activity inhibitory effect of the enzyme ACE (angiotensin converting enzyme) involved in the induction of hypertension.

The present invention relates to an extract for antihypertensive which is extracted from Korean mistletoe by hot water. The present invention is an antihypertensive extract which is further extracted with an organic solvent after the hot water extract by cutting the Korean mistletoe. In another aspect, the present invention is a pharmaceutical composition for antihypertensive containing Korean mistletoe extract as an active ingredient. The present invention is a functional food or food additive for antihypertensive using Korean mistletoe extract as an active ingredient.

It is an object of the present invention to provide a method for preparing Korean mistletoe extract and an inhibitory effect of hypertension by the extract.

According to the present invention, the Korean mistletoe is washed with water, dehydrated naturally, and then chopped, and 5 times distilled water is added to the weight, followed by boiling for 2 hours, followed by heat extraction. The supernatant obtained by centrifugation is filtered with filter paper (0.45 μm filter paper) and frozen It provides dried sterile Korean mistletoe heat extract (HKM-110).

Hereinafter, the present invention will be described in detail with reference to examples. The following examples are provided to explain the present invention, but the present invention is not limited thereto.

Example 1 Antihypertensive Activity by Continuous Oral Administration of 30 mg of HKM-110

Antihypertensive activity by HKM-110 was examined in animal experiments using SHR, which was born with high blood pressure. HKM-110 dissolved in physiological saline in SHR was orally administered 30 mg once a day for 3 days per animal, and physiological saline was administered orally in the control group. Blood pressures of control and treatment groups were measured on a Pulse Amplifier (Model 29, USA). As a result, a significant drop in blood pressure was observed from day 2 after the start of oral administration of HKM-110 at 30 mg / day, and the effect of blood pressure drop by oral administration of HKM-110 lasted until day 7 after the start of administration. (See Figure 1).

Example 2 Antihypertensive Activity by 10 mg HKM-110 Continuous Oral Administration

In order to investigate the change of antihypertensive activity according to the dose of HKM-110, the dose was decreased compared to that of Example 1 and orally administered to rats, and then the effect of lowering blood pressure was measured. HKM-110 dissolved in physiological saline was orally administered 10 mg once a day for 3 days per animal and blood pressure was measured at an interval of 1 day, even when HKM-110 was orally administered at 10 mg / day. A significant drop in blood pressure was observed from day 1, and it was confirmed that the effect of lowering blood pressure by HKM-110 administration lasted until 6 days after administration (see FIG. 2). From the results of Examples 1 and 2, oral administration of HKM-110 induces an antihypertensive effect, and antihypertensive activity by HKM-110 was confirmed to be effective even at a dose of 10 mg / day.

Example 3 Negative Antihypertensive Activity of HKM-110 Solution

In order to investigate whether antihypertensive activity is induced even by continuous intake of HKM-110, HKM-110 was dissolved in water and measured freely in SHR so as to be ingested 20 mg per day. Blood pressure was measured using a Pulse Amplifier (Model 29, USA) for 35 days at 7-day intervals from the rats of the HKM-110 solution-negative and water-only control mice. As a result, a significant drop in blood pressure was observed in the HKM-110-negative rats, and it was confirmed that the blood pressure-lowering effect by HKM-110 continued for a period of negative numbers (35 days) (see FIG. 3).

Example 4 ACE Inhibitory Activity

As an onset of hypertension, angiotensin-I is cleaved at the C-terminus of Angiotensin I by the action of angiotensin converting enzyme (ACE). It has been found that blood pressure is elevated by promoting resorption of ions. In the present invention, the blood pressure lowering effect of HKM-110 was analyzed in terms of ACE inhibitory activity. After adding the ACE coenzyme solution to the substrate solution (having the Hip-His-Leu structure) for ACE, the reactant produced by the ACE degradation reaction was measured by absorbance at 228 nm. As a result of investigating the inhibitory activity of ACE by reacting various concentrations of HKM-110 with the ACE substrate solution, HKM-110 exhibited concentration-dependent ACE inhibitory activity (see FIG. 4). From these results, it was confirmed that the mechanism of action of antihypertensive activity by HKM-110 is related to the inhibition of ACE enzyme activity.

As described above, the present invention relates to antihypertensive activity by the Korean mistletoe extract, the inhibitory effect against hypertension according to the present invention is due to the hydrothermal extract of Korean mistletoe. It was confirmed that the antihypertensive activity of the Korean mistletoe extract was expressed not only by oral administration once a day but also by continuous intake through negative drinking water. In addition, the mechanism of action of antihypertensive activity by HKM-110 was associated with inhibition of ACE activity. In addition, since the antihypertensive activity of Korean mistletoe extract was induced by oral administration, it can be used as a functional food or food additive, and especially as an injection that can be used for the prevention and treatment of hypertension, including a pharmaceutical composition by oral administration. Application is possible.

Claims (4)

Antihypertensive extract, characterized in that the hot water is extracted by cutting the Korean mistletoe. The antihypertensive extract according to claim 1, wherein the Korean mistletoe is chopped and extracted with hot water, followed by further extraction with an organic solvent. A pharmaceutical composition for antihypertension, comprising the Korean mistletoe extract described in claim 1 as an active ingredient. Functional food or food additives for antihypertensive, characterized in that using the Korean mistletoe extract described in claim 1 as an active ingredient.