KR20040060749A - Cpt-1 유전자의 발현을 특이적으로 전사 단계에서조절하는 화학물질의 스크리닝 방법 및 그 방법으로검색된 제니스테인을 유효 성분으로 포함하는 cpt-1발현 촉진제 - Google Patents
Cpt-1 유전자의 발현을 특이적으로 전사 단계에서조절하는 화학물질의 스크리닝 방법 및 그 방법으로검색된 제니스테인을 유효 성분으로 포함하는 cpt-1발현 촉진제 Download PDFInfo
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- KR20040060749A KR20040060749A KR1020030095226A KR20030095226A KR20040060749A KR 20040060749 A KR20040060749 A KR 20040060749A KR 1020030095226 A KR1020030095226 A KR 1020030095226A KR 20030095226 A KR20030095226 A KR 20030095226A KR 20040060749 A KR20040060749 A KR 20040060749A
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
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Abstract
Description
프라이머 종류 | 서열 번호 | 작용 제한효소 | 서열 (밑줄은 제한효소 인식부위) | |
P1(-29 kb) | 포워드 프라이머 | 1 | Kpn I | 5'GGGGTACCCCGAATGAATGAATGAACGAATG 3' |
리버스 프라이머 | 2 | BamHI | 5'CGGGATCCCGCGTGCGCATAAAATCGC 3' |
Claims (7)
- CPT-1 프로모터와 루시퍼라제를 포함하는 재조합 플라스미드.
- 제 1항에 있어서, 하기와 같은 유전자 지도를 갖는 재조합 플라스미드 pCPT-1P-luc.
- 제 1항의 플라스미드로 형질전환된 Huh7 세포주 (수탁번호: KCLRF-BP-00090).
- 제 3항의 세포를 이용하는 것을 특징으로 하는, CPT-1 유전자의 발현을 특이적으로 전사 단계에서 조절하는 화학물질의 스크리닝 방법.
- 제 4항에 있어서,a) 시험하고자 하는 화합물질과 제 3항의 세포를 접촉시키는 단계;b) 단계 a)에서 발생한 시그널의 양을 정량적으로 측정하는 단계; 및C) 단계 b)에서 측정한 시그널 양을 대조군의 시그널 양과 비교함으로써, 상기 시험 화학물질이 당해 유전자의 발현을 특이적으로 전사단계에서 조절하는지의 여부를 결정하는 단계를 포함하는, CPT-1 유전자의 발현을 특이적으로 전사 단계에서 조절하는 화학물질의 스크리닝 방법.
- 제 4항의 스크리닝 방법으로 검색된 제니스테인을 유효 성분으로 포함하는 CPT-1 발현 촉진제.
- 제 4항의 스크리닝 방법으로 검색된 제니스테인을 유효 성분으로 포함하는 지방산의 베타 산화 촉진제.
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KR20020085064 | 2002-12-27 | ||
KR1020020085064 | 2002-12-27 |
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KR20040060749A true KR20040060749A (ko) | 2004-07-06 |
KR100994632B1 KR100994632B1 (ko) | 2010-11-15 |
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KR1020030095226A KR100994632B1 (ko) | 2002-12-27 | 2003-12-23 | Cpt-1 유전자의 발현을 특이적으로 전사 단계에서조절하는 화학물질의 스크리닝 방법 및 그 방법으로검색된 제니스테인을 유효 성분으로 포함하는 cpt-1발현 촉진제 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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KR100821457B1 (ko) * | 2006-11-24 | 2008-04-11 | (주)아모레퍼시픽 | Idh3a 프로모터 및 루시퍼라제 함유 벡터로형질전환된 세포주, 및 그를 이용한 비만억제 후보물질검색방법 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
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US6544566B1 (en) * | 1999-04-23 | 2003-04-08 | Protein Technologies International, Inc. | Composition containing plant sterol, soy protein and isoflavone for reducing LDL cholesterol |
AU2001253070A1 (en) * | 2000-03-31 | 2001-10-15 | Jonathan Ingram | Isoflavones for treatment of obesity |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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KR100821457B1 (ko) * | 2006-11-24 | 2008-04-11 | (주)아모레퍼시픽 | Idh3a 프로모터 및 루시퍼라제 함유 벡터로형질전환된 세포주, 및 그를 이용한 비만억제 후보물질검색방법 |
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