KR20040060497A - Granule tea of Sorbus commixta and manufacturing method thereof - Google Patents

Abstract

PURPOSE: A method of making granular tea containing a Sorbus commixta Hedlund extract as an effective ingredient is provided. It permits a consumer to easily take a useful component contained in Sorbus commixta Hedlund in tea form. CONSTITUTION: Sorbus commixta Hedlund is washed with water, ground and concentrated. The extract containing 3.22 to 9.22% by weight of a Sorbus commixta Hedlund concentrate with a Brix from 40 to 45 is mixed with an optional ingredient and granulated. The optional ingredient contains 17.23% by weight of lactose, 61.75 to 67,75% by weight of purified glucose, 8.7% by weight of sorbitol, 2.26% by weight of a jujube concentrate and 0.84% by weight of flavoring materials.

Description

Granule tea of Sorbus commixta and manufacturing method

The present invention relates to rowan granule tea and a preparation method thereof.

Sorbus commixta is a deciduous arborescent belonging to the Rosaceae family, reaching 4 to 6 m in height and distributed natively between 500 and 1,200 m above sea level in various parts of Korea. Rowan grows in relatively high deciduous forests, and its distribution is not recognized in summer, but during the red leaf period of October-November, there are fruits of shipping color and yellow-yellow foliage.

The leaves of the rowan are alternate, the upper right lobes are 13-15 small leaves, lanceolate, and sawtoothed. Rowan's winter snow has sticky mucus. The flowers of rowan are white in June, the fruits are 8-10mm in diameter, and ripen red in October.

The components in rowan tree vary according to the following types.

(1) Fruits: Fresh herbs contain vitamin C, flavonoids, catechol, carotene, β-carotene epoxide, citric acid, malic acid, succinic acid, etc., and sugars include glucose, fructose, sucrose, and sorbitol. It is contained in ursolic acid as a triterpenoid. Contains amino acids, caffeine, clogenic acid, neoclogenic acid, isocclogenic acid, coumarin, etc.

(2) Leaves: contains vitamin C, flavone derivatives, isoquecetin-3-sophoroside, istragalin, aspartalin, camphorol, quercerine-3-phosphoside, etc. .

(3) White matter (白 木質): Lignan-xiloside, β-sitosterol, methoxyaucuparin, aucuparin is contained.

(4) Flowers: contains L-leucine, L-varin, isobutylamine, etc. In addition, when the fruit matures, the content of aliphatic acid decreases, while the content of aromatic acids rises. In the chloroform extract fraction of fruit, ursolic acid precipitates as white, amorphous crystals. On the other hand, water-soluble pectin is contained when the fruit ripens, and when it is green, it exists as protopectin.

On the other hand, the leaves, stems and berries of rowan are known to contain the general and mineral components such as moisture, protein, carbohydrates, ash as shown in Tables 1 and 2 below.

Table 1. Measurement of general components of leaves, stems and berries of rowan (unit:%)

division moisture protein Geology carbohydrate Ash Sugar fibrin rowan leaf 10.4 10.9 5.7 51.8 13.6 7.6 stem 9.0 3.6 0.3 43.1 42.0 2.0 Fruit 24.7 5.8 4.2 47.4 14.6 3.3

Table 2. Mineral Measurements of Leaves, Stems and Berries of Rowan (unit: mg / 100g)

division P K Ca Fe Na rowan leaf 127.9 1798.4 1093.8 - - stem 104.9 383.6 502.8 - - Fruit 375.3 1103.9 435.1 8.5 5.3

As above, the subject tree has a useful ingredient in each area. In particular, the fruit extract of Asters lowers the body's liver lipid and lowers the serum cholesterol level. In addition, it is effective in scurvy due to its high content of vitamin C, and flavonoids contained in Asters prevent the weakening of blood vessel walls, and sorbitol can be used as a diuretic because of its strong osmotic diuretic effect.

Although the acanthus containing the useful components as described above can be used in various fields, there are almost no examples of using them in industry. This may be due to the fact that aquifoliaceae is not a common plant, and also because it grows in high places and is not readily available.

Meanwhile, as related art of the present invention, there is a manufacturing method of a tea using the fruit of the Japanese Laid-Open Patent No. 2001-0055831 as a prior art using an apple, but its technical configuration is different from the present invention.

It is an object of the present invention to provide a rowan granule tea comprising the mountain ash extract as an active ingredient and a method for preparing the same, so that useful components contained in rowan tree can be easily consumed in the form of tea.

The present invention includes a rowan granule tea containing the mountain ash extract as an active ingredient and a preparation method thereof.

In the rowan granule tea of the present invention, the rowan extract is preferably added 3.22 to 9.22% by weight. If added to less than 3.22% by weight is not enough to exhibit the functionality of the rowan, when it exceeds 9.22% by weight because the characteristic of rowan is strong and may not be desirable in terms of palatability. However, since the invention is not impossible even if somewhat out of the above range, the present invention includes equivalent areas within these ranges.

In a preferred embodiment of the present invention, the rowan extract is provided in the form of a concentrate of about 44 Brix. When the concentrated liquid is extracted from the rowan, hot water is added about 5 to 10 times the weight of the rowan and extracted several times for 5 to 12 hours at about 70 ° C to 90 ° C, and the extracted rowan is compressed to remove the residue. The extract can be obtained by concentrating approximately 40-45 Brix. The present invention is carried out to concentrate the rowan extract to about 44 Brix, but this is only a preferred embodiment of the present invention to the extent that does not mean that the implementation of the mountain ash concentration slightly beyond this.

The method for producing granules granule tea of the present invention

Washing and drying the rowan, and then grinding

Extracting and concentrating the ground rowan,

Mixing the concentrated rowan extract and subsidiary material,

Granulating the mixture of concentrated rowan extract and submaterial.

In the rowan granule tea of the present invention, the rowan extract as the main material can be extracted from any one selected from the leaves, stems, roots or from a mixture of two or more uniformly mixed.

Rowan extract of the present invention is sufficient to extract using a known extraction method and does not require a special limitation. Therefore, known hot water extraction or extraction using an organic solvent, for example, alcohols and the like may all correspond to this. On the other hand, in order to improve the extraction effect of useful components contained in rowan may be carried out in parallel with the extraction of organic solvent using hot water extraction and alcohols.

In the present invention, rowan extract is concentrated to about 40 to 45 Brix, and the concentrate is 3.22 to 9.22% by weight. And the like. Also, flavoring may be added to improve tea functionality. Such fragrances can generally use known fragrance ingredients which can improve tea functionality.

The addition amount of the submaterial is a matter that can be appropriately selected by those skilled in the art and does not require any particular limitation. For example, in the present invention, 17.23 wt% of lactose, 61.75 to 67.75 wt% of refined glucose, 8.7 wt% of sorbitol, 2.26 wt% of jujube concentrate, and 0.84 wt% of fragrance may be added.

Hereinafter, the present invention will be described by the following experimental examples, examples and test examples. However, these are only examples of the present invention, and the scope of the present invention is not limited thereto.

Experimental Example 1 Measurement of Cytotoxicity and Mutagenesis of Rowan

Normal cytotoxicity against leaves, stems and fruits of rowan was measured by SRB-assay, which measured cell proliferation and toxicity by staining cellular proteins, and mutagenesis was measured by Rec-assay. In summary.

Human normal liver cells (WRL-68) were used in the cytotoxicity measurement for normal cells. The basal medium used for cell culture was cultured by adding 10% in RPMI 1640 (GIBCO, USA). The initial concentration of the cells was adjusted to a concentration of 4 × 10⁴ cells / ml was used to inoculate 100 ㎕ / well in 96 well tissue culture microplate.

After adjusting the cells to a concentration of 2-3 × 10 μs cells / ml, incubate the medium containing 100 μl of cells into each well of the 96 well tissue culture micrqplate and incubate in 37 ° C. and 5% CO₂ incubator for 24 hours. Then, the leaves, stems and berries of rowan were added in 100% each of the extract of the hot water extract in a conventional manner and incubated for 48 hours. After incubation and staining (SRB), the absorbance of each well was measured using a microplate reader at 540 nm, and the average is shown in Table 3 below.

Mutagenesis measurements were performed as follows.

Bacillus subtilis PB 1652 rec + (repair proficient) and PB 1791 rec- (repair deficient), which were distributed from Franca Zani, Italy by the Rec-assay method, were scrubbed in cleanbenches. Inoculated in a Petri dish solidified nutrient agar (nutrient agar) was incubated for 24 to 48 hours. When the incubation was completed, platinum was added to sterile TSB liquid medium and incubated at 37 ° C. for 24 hours to use in the experiment.

10 ml of soft broth agar added with 0.1 ml spore solution was added to the petri dish and solidified. Then, the paper disk was placed and the extracts of rowan leaves, stems and fruits were extracted in the usual manner. 20 μl each was added, and 10 μl of the control MNNG was inoculated and incubated at 37 ° C. for 12 to 24 hours to measure mutagenicity and antimutagenicity.

The mutagenicity was sterilized in 20 µg / paper disc using a strong mutagenic substance MNNG (2 µg / µl) as a DNA-damaging activity control under sterile clean-bench. To the disc, add the leaves, stems and fruits of rowan, and extract the hot water extract in the usual manner to 4-100 μl / paper disc at a concentration of 1%, respectively, by rec (+) and rec (-) bacteria. Mutagenicity was measured by the difference in the suppressed area (cm) formed.

Antimutagenicity was determined by mutagenicity and then mixed with MNNG (10µg / p.disc) and Rowan extract (50, 100µg / p.disc) in 1: 1 (10µl: 10µl). After cultivation by means of the comparison of the inhibitory regions formed by each of the extract to determine the degree of mutagenicity of MNNG mutagenic material, the difference between the rec (+) and rec (-) bacteria (cm) and the difference rate ( sample + MNNG / MNNG). The control was treated only with MNNG. The results are shown in Table 3 below.

Table 3. Normal cytotoxicity and mutagenesis measurements for rowan (% / mg / mL)

division Concentration (%) Normal cytotoxicity Mutagenesis rowan leaf 1.0 18 1 or less stem 1.0 22 1 or less Fruit 1.0 20 1 or less Effect criteria 50% or more 1 or more Test method SRB-assay Rec-assay

As shown in Table 3, the rowan leaves are particularly poor in toxicity to normal cells and excellent in normal cytotoxicity-related properties, it can be seen that there is no significant difference in the leaves, stems and berries of the rowan for mutagenesis.

Experimental Example 2 Measurement of Cancer Cell Inhibition on Rowan

Cancer leaf inhibition rate of the leaves, stems and berries of the rowan was measured by SRB-assay and the results are summarized in Table 4 below.

In the cancer cell inhibition rate experiment, the cancer cells used were breast cancer cells MCF-7, lung cancer cells A549, and liver cancer cells Hep3B.

The initial concentration of each cancer cell was adjusted to a concentration of 4 × 10 μs cells / ml and used to inoculate 100 μl / well in 96 well tissue culture microplate.

After adjusting the cells to a concentration of 2-3 × 10 μs cells / ml, the medium containing 100 μl of cells was dispensed into each well of the 96 well tissue culture micrqplate and incubated for 24 hours in a 37.5 ° C., 5% CO₂ incubator. Then, the leaves, stems and berries of rowan were added to the extract of hot water in a conventional manner, each 100 μl of a concentration of 1% and incubated for 48 hours. After incubation and staining treatment (SRB) and then measured the absorbance of each well using a microplate reader at 540nm and the average is shown in Table 4 below.

Table 4. Cancer cell inhibition rate (% / mg / ml) for rowan

division Concentration (%) Breast Cancer (MCF-7) Lung Cancer (A549) Liver Cancer (Hep3B) rowan leaf 1.0 76 81 65 stem 1.0 80 83 76 Fruit 1.0 84 80 86 Effect criteria 50% or more 50% or more 50% or more Test method SRB-assay SRB-assay SRB-assay

As shown in Table 4, the inhibition rate of breast cancer cells and lung cancer cells has no significant difference in the leaves, stems and fruits of rowan, but the rowan fruit shows the best characteristics in the inhibition rate of liver cancer cells.

Experimental Example 3 Measurement of Adult Disease-Related Characteristics of Rowan

The characteristics of geriatric diseases such as hypoglycemia, hypertension inhibition, hepatic function improvement, immune activity and antioxidant activity according to the addition of leaves, stems and fruits of rowan were measured and the results are shown in Table 5 below.

(1) Blood sugar drop measurement

The hypoglycemic measurement was performed using α-glucosidase, which plays a decisive role in blood glucose elevation in vivo. First, enzyme was prepared by dissolving the enzyme in 10 mM PIPES buffer, and 10 µl of this enzyme solution, 40 µl of 20 mM maltose and 10 µl of the extract of hot water extracted from rowan leaves, stems, and berries in the usual manner at 1% concentration. The final volume was mixed with 60 μl and incubated at 37 ° C. for 20 minutes. 1 ml DNS reagent was added to 60 µl of the reaction solution, and the reaction was stopped by boiling water treatment (10 minutes) at 100 ° C. water, and then absorbance was measured at 540 nm to quantify the reducing sugar produced by the enzymatic reaction to calculate the enzyme activity inhibition rate.

(2) hypertension inhibitory ability

Since ACE (angiotensin converting enzyme) is an enzyme that induces hypertension, ACE reagent was used to measure the inhibitory activity of this enzyme. Before the experiment, all the reactants were maintained at 37 ° C., 1 vial of ACE reagent was dissolved in 10 ml of 37 ° C. distilled water, and 1 ml each was taken into a tube. 100 μl of 1% extract and ACE Calibrator, extracted with rowan leaves, stems and fruits, were added to each tube in the usual manner, and then reacted at 37 ° C. for 5 minutes to measure absorbance at 340 nm. After another 5 minutes, the absorbance was determined as final A.

As a control, 100 μl of distilled water was added instead of the extract. The ACE (U / L) value of the control was measured by the change in absorbance when nothing was added. The activity calculation of the ACE was calculated according to the following formula and the results are shown in Table 5 below.

ACE (U / L) = (Initial A-Final A) _test / (Initial A-Final A) _control ×

active of ACE reagent (50U / L)

(3) liver function improvement

In order to measure the degree of detoxification, the activity of GST (gultathion-S-transferase), one of the liver's important detoxification mechanisms, was measured. A reaction solution was added to the prepared reaction reagent as a control, and the extract of rowan leaves, stems and berries in the usual manner was added at a concentration of 1% and reacted at 37 ° C. for 5 minutes, followed by 1 as a substrate. After the addition of -chloro-2,4-dinitro benzene it was reacted again at 37 ℃ for 2 minutes. After the reaction, 20% TCA was added to terminate the reaction, and centrifuged. The supernatant was measured for absorbance at 340 nm, and the specific activity and activity ratio of GST were calculated as shown in Table 5 below.

Total activity (units) = (A ₃₄₀ / 9.6) × Dilution factor × (3ml / 0.1) ×

crude extract (ml)

Specific activity (units / ml protein) = total activity / total protein

% Activity = specific activity _test / specific activity _control × 100

(4) immune activity

After inoculating the experimental immune cell line Raji (RPM1-1640 medium) to reach the normal cell growth period (takes about 7 days), 96-well plate (100 μl / well of medium adjusted to 5 × 10 ⁴ Cells / ml) was 37 Incubated for 24 hours at 5 ℃ CO ₂ state. 100 µl samples of 1% concentrations of the extracts of rowan leaves, stems, and fruits were extracted in the usual manner, and then cultured for 48 hours at 37 ° C. with 5% CO ₂ , and then the supernatant was removed and cold 10% ( w / v) 100 μl of trichloroacetic acid (TCA) was added. After standing at 4 ° C. for 1 hour, washing with distilled water 4-5 times to remove TCA and drying the plate at room temperature, 0.4% (w / v) dissolved in 1% (v / v) acetic acid in each well. 100 μl of SRB solution was added and stained at room temperature for 30 minutes.

Unbound SRB dye solution was washed 4-5 times with 1% acetic acid and dried, and then 100 μl of 10mM Tris buffer was added to dissolve the dye solution and absorbance was measured at 540 nm. Cancer cell growth (%) = test / control × 100 is to measure the extent to increase the growth of immune cells Raji suggests that immunity can be increased as cell growth increases. Immune function enhancement effect was verified using B cell (Raji), a human immune cell. The growth of the cells was cultured at 5% CO ₂ , 37 ℃ in RPMI 1640 medium containing 10% FBS, the immune function enhancement effect was measured using the SRB assay and the results are shown in Table 5.

(5) antioxidant activity

In search of antioxidants, 5 ml of 4.5 × 10 ^-3 linoleic acid aqueous solution was added with 0.5 ml of 1% concentration of the extract of hot water extracted from rowan leaves, stems, and berries in the usual manner, and then incubated at 50 ° C. 1.1 ml of the test tube was collected every four days, and 1 ml of TCA, 2 ml of TBA, 0.1 ml of BHT, and 1 ml of SDS were mixed. Then, the mixture was blown with N ₂ , sealed, incubated in a boiling water bath for 15 minutes, allowed to cool, mixed with 1 ml of acetic acid and 2 ml of chloroform, followed by centrifugation at 2,500 rpm for 10 minutes, and the supernatant was measured for absorbance at 532 nm. Linoleic acid is an essential fatty acid and easily oxidized to polyunsaturated fatty acids. These foods, which exhibit antioxidant properties against linoleic acid, are effective in preventing aging and preventing adult diseases. The search for the antioxidant inhibitor was measured by TBA method and shown in Table 5 below.

Table 5. Gender-Related Characteristics (% / mg / mL) for Rowan

division Concentration (%) Hypoglycemic Hypertension Liver function improvement Immunity Antioxidant activity rowan leaf 1.0 57 57 210 170 52 stem 1.0 65 60 135 200 59 Fruit 1.0 57 49 112 140 60 Effect criteria 50% or more 50% or more More than 100% More than 100% 50% or more Test method α-glucosidase ACE system GST enzyme method SRB-assay TBA

<Example 1>

After washing and draining the dried leaves 1kg was crushed to a size of 0.5cm or less. Water was added 5 times to the weight of the crushed mountain ash, and extracted repeatedly for 5 to 12 hours at about 70 ℃ ~ 90 ℃, and the extracted mountain ash was compressed to obtain a rowan extract concentrated to 44Brix of the residue removed.

Rowan granule tea with 9.22% by weight of rowan extract concentrated as 44Brix as main ingredient, 17.23% by weight lactose, 61.75% by weight refined glucose, 8.7% by weight sorbitol, 2.26% by weight jujube concentrate, 0.84% by weight flavor. Was prepared.

<Example 2>

A rowan granule tea was prepared in the same manner as in Example 1, except that 6.22% by weight of rowan extract concentrated as 44Brix and 64.75% by weight of refined glucose in the submaterial were used as main ingredients.

<Example 3>

A rowan granule tea was prepared in the same manner as in Example 1, except that 3.22% by weight of rowan extract concentrated as 44Brix and 67.75% by weight of refined glucose in subsidiary materials were used as main ingredients.

<Test Example>

Brix, color, solubility, granularity, product yield and sensory test were measured as quality characteristics of the rowan granule tea prepared by the method of Examples 1 to 3 and the results are shown in Table 6 below. .

The sensory test was performed by 30 well-trained sensory testers who measured color, aroma, taste, and overall acceptability on a five-point scale.

Table 6. Characteristics of rowan granule tea

process Brix Chromaticity * Solubility (sec) Granulation degree Product yield (%) Sensory test ** (1 ~ 5) L a b color incense flavor Synthesis Example 1 10.8 53.87 14.71 52.08 4 Good 84 4 4 4 4 Example 2 10.9 52.60 15.77 53.53 4 Good 85 5 4 5 5 Example 3 10.8 65.94 9.48 46.09 4 Good 85 3 3 3 3

* L: (+) white, (-) black, a: (+) Red, (-) Green, b: (+) Yellow, (-) Blue

** 1: Very bad, 2: Bad, 3: Normal, 4: Good, 5: Very good

The present invention can be easily ingested useful components contained in rowan by providing a granular tea containing the mountain ash extract as an active ingredient to make it easy to drink.

Claims (5)

Rowan granules tea comprising rowan extract as an active ingredient. The rowan granule tea according to claim 1, wherein the rowan extract comprises 3.22 to 9.22% by weight of a concentrate of about 40 to 45 Brix. Washing and drying the rowan, and then grinding Extracting and concentrating the ground rowan, Mixing the concentrated rowan extract and subsidiary material, A method for producing rowan granules, characterized in that it comprises the step of granulating a mixture of concentrated mountain ash extract and subsidiary material. The method for preparing rowan granule tea according to claim 3, wherein the rowan extract is extracted from one selected from leaves, stems, and roots or from a mixture of two or more. The ash extract according to claim 3, wherein the extract is about 40-45 Brix, 3.22-9.22% by weight of the main ingredient, 17.23% by weight lactose, 61.75-67.75% by weight of refined glucose, 8.7% by weight sorbitol, 2.26% by weight of jujube , Flavoring method of producing rowan granules tea comprising 0.84% by weight.