KR20040043775A - Composition comprising the extract of angelica purpuraefolia chung having potent anticancer activity - Google Patents

Abstract

PURPOSE: Provided is a composition which comprises the extract of Angelica purpuraefolia Chung having potent anticancer activity, thereby inhibiting the growth of cancer cells, and inducing the inhibition activity of the growth of a solid tumor cell. Therefore, it is useful for remedies or health foods useful for prevention and treating various kinds of cancers, such as skin cancer, pancreas cancer, blood cancer and the like. CONSTITUTION: A crude extract of Angelica purpuraefolia Chung has prevention and treatment effects on cancers. It is obtained by using the polar solvent selected from water, methanol or their mixture, or the non-polar solvent selected from hexane, chloroform, ethyl acetate as an extraction solvent. A pharmaceutical composition contains the crude extract of Angelica purpuraefolia Chung as an active ingredient, pharmaceutically acceptable carrier.

Description

Geological activity extract having anticancer activity and composition comprising same {Composition comprising the extract of Angelica purpuraefolia Chung having potent anticancer activity}

The present invention relates to crude extracts of Angelica purpuraefolia Chung, anti-polar solvent soluble extracts having anticancer activity and compositions containing them.

Cancer is one of the incurable diseases to be solved by humans, and huge capital is being invested in the development to cure it all over the world, and it is the number one cause of disease death in our country. About 60,000 people are dead. Carcinogens that cause cancer include smoking, ultraviolet rays, chemicals, foods, and other environmental factors, but the causes of various cancers are difficult to develop, as well as the effects of treatments vary depending on the site of occurrence. The substances currently used as therapeutic agents are highly toxic and cannot selectively remove only cancer cells, suggesting that cancer prevention (chemoprevention) conquers cancer rather than countermeasures after cancer. It would be a much more efficient way under the premise.

Research methods of anticancer agents for treating cancer have recently been investigated, such as searching for a direct cytotoxic substance to cancer cells, searching for a substance that modulates the immune ability of the living body, searching for a substance that inhibits the metastasis of cancer cells, and recently Various methods are in progress, such as the search for a substance that inhibits angiogenesis, and in order to prevent cancer, various mechanisms of cancer development are effectively blocked, thereby preventing normal cells from transferring to cancer cells or carcinogenic mechanisms. Substances that can delay or recover the process of the drug may be used, which should be free of short or long term toxicity, be easy to take and inexpensive. The incidence of cancer is known to be very complex and progresses through several stages. Understanding the mechanism of action of carcinogenesis enables the development of drugs for the prevention and treatment of cancer.

At present, there is an urgent need for the development of a new concept of cancer prevention strategy, a cancer prevention substance, and the development of such a cancer prevention substance can be very useful for inhibiting or treating all diseases as well as cancer. For this reason, as the development of cancer prevention substances is recognized as a new direction of cancer research and attention is focused, the development of cancer prevention substance materials is active not only in the real medicine field but also in the food chemistry field. The situation is made of, and is actually introduced in the food field.

Examples of active substances associated with the prevention of reported cancers are β-carotene (Suda, Carcinogenesis , 7 , pp711-715, 1986), and ceselin type coumarin (Nishino, Carcinogenesis , 11 , pp1557- 1561, 1990), curcumin isolated from Curcuma longa (Rao, Carcinogenesis, 14 , pp2219-2225, 1993), myristicin isolated from parsley (Zheng, Carcinogenesis , 13). , pp1921-1923, 1992) and epigallocatechin-3-gallic acid of green tea (Katiyar, Nutrition and Cancer , 18 , pp73-73, 1992).

In addition, according to the International Reports of Medicinal Chemistry, 19 out of 31 newly approved anticancer drugs from 1983 to 1994, about 61% of drugs were developed separately from natural products or were derived from semisynthetic derivatives or natural models. Among the 93 anticancer drugs that have been developed and used until 1994, six drugs from biological sources such as interferon and interleukin-2 are known as paclitaxol, vincristine and vin. 15 drugs from natural sources such as vinblastin, doxorubicin, 25 semisynthetic derivatives of natural products such as etoposide, irinotecan hydrochloride, cisplatin , 33 pure compounds like amsacrine, cytosine arabinoside, fluorouracil There were 14 drugs that were synthetic but originated from natural models.

As described above, substances with cancer prevention or therapeutic activity have already been reported from various plant extracts, and thus, a substance with a cancer prevention agent is highly likely to be searched for from plant extracts, and plant extracts are toxic by long-term use. In the anti-cancer activity detection of plant extraction in the development of cancer prevention and treatment, and the isolation and activity screening of pure substances according to the low risk, the easy production of raw materials, and the preservation state are relatively stable to the external environment. This approach has many advantages, such as high efficiency.

As used in the present invention, Angelica purpuraefolia Chung is a perennial plant of the genus Minamidae (Umbelliferae), called Namgang bow, canopy ear, and Namdang ear , and inhabits a lot at altitude of 1500m above sea level of Jiri, and is used as a medicinal plant as a poisonous plant. In some cases, it is used to treat dermatitis and inflammation.

However, despite much research and interest in natural substances exhibiting cancer prevention efficacy, the geological activity extract of the present invention has no known efficacy of research, treatment and prophylaxis for cancer diseases, and targets various kinds of natural products. In the process of searching for plants that are expected to be useful for cancer prevention and treatment, geological activity showed a significant inhibitory effect on cancer cell lines.

Accordingly, the present inventors have completed the present invention by confirming that geological activity extract has excellent cancer prevention and treatment effect by inhibiting the growth of cancer cells while continuing to study the separation and purification of plant components.

The present invention is to provide a pharmaceutical composition and health supplement for the treatment and prevention of cancer diseases containing geographic activity crude extract, a polar or non-polar solvent soluble extract having anticancer activity.

1 is a chromatogram of HPLC analysis of constituent extracts of geographic activity chloroform soluble extract of the present invention,

2 is a diagram showing the solid cancer cell line inhibitory effect of the untreated group, the positive control group treated with the anti-cancer drug adriamycin, geography active extract administration group (125, 250mg / ㎏) in the animal model.

In accordance with the above object, the present invention provides a crude extract of Angelica purpuraefolia Chung, a polar or nonpolar solvent soluble extract effective for the prevention and treatment of cancer diseases.

The crude extract means an extract available in a polar solvent selected from water, methanol, butanol or a mixed solvent thereof.

The polar solvent soluble extract means an extract soluble in a polar solvent selected from water, methanol, butanol or a mixed solvent thereof, and the non-polar solvent soluble extract means an extract soluble in a nonpolar solvent such as ethyl acetate, chloroform or hexane.

The method of separating the crude extract, polar or nonpolar solvent soluble extract from the geographic activity of the present invention is as follows.

Hereinafter, the present invention will be described in detail.

Geographically active extract of the present invention is about 5 to 25 times, preferably about 10 to 15 times the water, methanol or ethanol lower alcohol or about 1: 1 of the underground weight (kg) of gelatinous ribs cut in the shade. Mixed solvents thereof having a mixing ratio (kg / L) of 0.1 to 1:10, preferably 1: 0.2 to 1: 5, still more preferably 5 to 80 ° C, preferably 30 to 90 to 100% methanol Soluble extract according to water, lower alcohol or a mixed solvent thereof by an extraction method such as solvent extraction, singular hydrothermal extraction or ultrasonic extraction for about 15 minutes to 2 days, preferably 30 minutes to 12 hours at an extraction temperature of 55 ° C. Phosphorous crude extract can be obtained by extraction, concentration under reduced pressure and lyophilization.

The present invention provides a composition for the prevention and treatment of cancer diseases, including crude extracts obtained in the extraction process.

In addition, the polar or non-polar solvent soluble extract purified from the active ingredient of the present invention, after suspending the crude extract in water, it is about 1 to 100 times, preferably about 1 to 5 times the volume of n -hexane, chloroform or By adding a nonpolar solvent such as ethyl acetate, the aqueous layer and the nonpolar solvent soluble layer can be obtained by fractionation of 1 to 10 times, preferably 2 to 5 times, and may also be carried out in a conventional fractionation process (Harborne). JB Phytochemical methods: A guide to modern techniques of plant analysis . 3rd Ed. Pp 6-7, 1998).

Hereinafter, the separation process of the polar solvent and the non-polar solvent soluble extract of geographic activity,

For example, a first step of cold extraction of a geographic activity with a polar solvent such as water, methanol or butanol, followed by filtration and concentration under reduced pressure and lyophilization to obtain a crude extract soluble in a polar solvent;

The crude extract is suspended in water, dissolved in a non-polar solvent such as hexane, chloroform, ethyl acetate, and then suspended. The crude extract is fractionated and filtered with a separatory funnel to obtain a non-polar solvent soluble extract by sequential fractionation according to the polarity of the solvent. Two steps;

A third step of sequentially fractionating and filtering the aqueous layer using a polar solvent such as butanol or water to obtain a butanol soluble extract and a water soluble extract;

And a fourth step of concentrating and drying the chloroform soluble extract, ethyl acetate soluble extract, butanol soluble extract, and water soluble extract under reduced pressure.

Crude extracts, chloroform soluble extracts, ethyl acetate soluble extracts, butanol soluble extracts and water soluble extracts obtained by the above method were tested for cancer cell growth inhibitory activity and solid cancer cell inhibition-inducing effect in vitro, and thus showed significant inhibitory activity. These can be usefully used for the prevention and treatment of various cancer diseases, particularly skin cancer, pancreatic cancer, blood cancer, etc. related to the cancer cell inhibitory activity.

The present invention provides crude extracts, polar and non-polar solvent soluble extracts obtained by the above-mentioned preparation and effective for the prevention and treatment of cancer diseases.

In another aspect, the present invention provides a pharmaceutical composition containing the above geological activation crude extract, a polar or non-polar solvent soluble extract as an active ingredient, and effective in the prevention and treatment of cancer diseases, including a pharmaceutically acceptable carrier.

The composition for preventing and treating cancer diseases of the present invention comprises 0.1 to 20% by weight of the crude extract, the polar solvent or the nonpolar solvent soluble extract based on the total weight of the composition, the daily dosage of the composition is 125 to 1 kg body weight Provide 250 mg of pharmaceutical composition.

The cancer may include lung cancer, bone cancer, pancreatic cancer, skin cancer, blood cancer, head or neck cancer, skin cancer, uterine cancer, ovarian cancer, rectal cancer, gastric cancer, colon cancer, breast cancer, vulvar carcinoma, esophageal cancer, small intestine cancer or one or more combinations of these cancers. It means to include.

The present invention also provides an adjuvant for cancer treatment, which has a geological activation crude extract, a polar or non-polar solvent soluble extract as an active ingredient, and inhibits cancer cell development.

Compositions containing geological activity extract of the present invention may further comprise suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions.

Compositions comprising extracts according to the invention are formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral preparations, suppositories and sterile injectable solutions, respectively, according to conventional methods. Carriers, excipients and diluents which may be used in combination with the extract, and which may be included in the composition comprising the extract include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin , Calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and such solid preparations include at least one excipient such as starch, calcium carbonate, and sucrose in the gelatin extract. (sucrose), lactose (lactose), gelatin, etc. are mixed and prepared. In addition to simple excipients, lubricants such as magnesium styrate talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The amount of the extract of the present invention may vary depending on the age, sex, and weight of the patient, but in general, an amount of 1 to 500 mg / kg, preferably 100 to 300 mg / kg, may be divided once or three times daily. Can be. In addition, the dosage of the extract may be increased or decreased depending on the route of administration, the degree of disease, sex, weight, age, health status, diet, administration time, excretion rate, severity of the disease, etc. It does not limit the scope of.

The pharmaceutical composition of the present invention can be administered to mammals such as rats, mice, livestock, humans by various routes. All modes of administration can be expected, for example by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or intracerebroventricular injection.

The present invention provides a dietary supplement comprising a crude extract of geological activity and a food-acceptable food supplement additive exhibiting a preventive effect of cancer diseases.

In addition, the present invention provides a dietary supplement comprising a polar solvent or a non-polar solvent soluble extract of geological activity that exhibits a prophylactic effect of cancer disease, and a food acceptable additive.

It provides a health supplement beverage comprising an active extract of Angelica koreana MAX in the geologically active crude extract, polar or non-polar solvent soluble extract.

Compositions comprising the extracts of the present invention can be used in a variety of drugs, food and beverages for the prevention of cancer diseases. Examples of the food to which the crude extract, polar solvent and non-polar solvent soluble extract of the present geographic activity can be added include, for example, various foods, meat, chocolate, confectionery, pizza, ramen, other noodles, ice cream, alcoholic beverages, beverages. , Gums, teas, vitamin complexes, dietary supplements, and the like, and may be used in the form of powders, granules, tablets, capsules, or beverages.

Toxicity and side effects of geological active crude extract, polar solvent and non-polar solvent soluble extract itself of the present invention at a certain concentration or higher in animal experiments, for example, crude extracts appear at 350 to 400 mg / kg or more, so they are used for the prevention of cancer. It can be used at the right concentration.

The extract of the present invention may be added to food or beverages for the purpose of preventing cancer diseases. At this time, the amount of the extract in the food or beverage is generally added to the health food composition of the present invention 0.01 to 15% by weight of the total food weight, the health beverage composition is 0.02 to 10 g, preferably based on 100 ml Can be added in a ratio of 0.3 to 1 g.

The health beverage composition of the present invention has no particular limitation on the liquid component except for containing the extract as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks. Examples of the above-mentioned natural carbohydrates are conventional monosaccharides such as disaccharides such as glucose and fructose, such as maltose, sucrose and the like, and polysaccharides such as dextrin, cyclodextrin and the like. Sugars and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of natural carbohydrates is generally about 1-20 g, preferably about 5-12 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and salts thereof. , Organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. The compositions of the present invention may also contain pulp for the production of natural fruit juices and fruit juice beverages and vegetable beverages. These components can be used independently or in combination. The proportion of such additives is not so critical but is generally selected from the range of 0 to about 20 parts by weight per 100 parts by weight of the composition of the present invention.

The present invention is described in more detail based on the following examples, although the present invention is not limited thereto.

Example 1 Method of Producing Geographically Active Crude Extracts

After removing the foreign geological ligaments indigenous to the various fields in Korea used in the present invention, foreign substances were removed, and 83 g of geographic limbs were cut into pieces, and 1 liter of 100% methanol was added and cold-extracted at room temperature. The supernatant was filtered to remove the residues . The extract was filtered on filter paper and concentrated under reduced pressure with a vacuum condenser (EYLA, Japan) to obtain 5.4 g and use it as a sample.

Example 2 Method for Producing Geographically Active Polar Solvents and Nonpolar Solvent Soluble Extracts

After 5.4 g of the gelatinous active crude extract prepared in Example 1 was suspended in 300 ml of water, 600 ml of chloroform was added to the separatory funnel, and fractionated into a chloroform insoluble layer (upper layer) and a chloroform soluble layer (lower layer) to form chloroform. Soluble parts were collected. The same volume of chloroform solvent as in the upper layer (water layer) was repeated five times in the same manner as described above to obtain a substance that can be dissolved in the chloroform layer as much as possible until the color of the solution became light. Then, 3.04 g of chloroform extract of geological activity was added. Obtained and used as a sample, ethyl acetate was fractionated in the same manner as in the above chloroform to obtain 0.96 g of ethyl acetate soluble extract was used as a sample. Butanol and water were sequentially added to the aqueous layer and fractionated to obtain 0.43 g of butanol extract and 0.47 g of water extract, which was used as a polar solvent soluble extract sample of geographic activity.

Reference Example 1. Instrument Analysis

For analysis of cancer cell growth inhibitory components, reversed phase high performance liquid chromatography (Reversed-phase HPLC, SCL10A, Shimadzu Co., Japan) was used.

As a starting point, the solvent was a mixture of methanol and water in the same ratio as the mobile phase condition of HPLC, and the time was 20 minutes until the ratio of methanol and water became 9: 1, and the time of 30 minutes until methanol became 100%. Put it. At this time, the flow rate of the mobile phase passing through the reverse phase column was set to a flow rate of 2ml / min at room temperature, and reconfirmed by performing thin layer chromatography (TLC) together.

Geologically active chloroform soluble extract prepared in Example 2 was carried out in the same manner as above to analyze the components (see FIG. 1).

Experimental Example 1. Test of cancer cell growth inhibition effect in vitro

In order to determine the cancer cell growth inhibitory effect of the crude extract, polar or non-polar solvent soluble extract of geographic activity prepared in Examples 1 and 2 was carried out as follows.

After culturing the mouse pancreatic cell line MS 1 (ATCC, USA) and human blood cancer cells HL60 cell line (ATCC, USA), 0.5% dimethylsulfoxide (DMSO) as a negative control, and a positive control Camptothecin (final concentration 0.5 μg / ml) was treated and subject test substances were treated by concentration. After 48 hours of incubation, the solution was dissolved in lysis buffer, and the reaction mixture containing MTT was added thereto and reacted in an incubator at 37 ° C. for 50 minutes, and then the absorbance was measured at 610 nm using a microplate reader ( Scott, G, J. Clin.Microbial . , 36 , pp 362-366, 1998).

As a result, geological activation crude extract showed 50% cell growth inhibition of MS 1, which is a mouse pancreatic cell line, at 62µg / ml, and 50% cell growth inhibition rate of HL60 cell line of human blood cancer cells at 10µg / ml. Chloroform soluble extract showed a 50% cell growth inhibition rate at 8 μg / ml concentration against HL60 cell line of human blood cancer cells, showing an excellent cancer cell growth inhibitory effect (see Table 1).

Cancer cell Sample processing group 50% cell growth inhibitory concentration (㎍ / ㎖) MS-1 Crude extract 62 HL60 Crude extract 10 HL60 Chloroform Soluble Extract 8 Anticancer drug (camptothecin) Crude extract 0.36

Experimental Example 2. Anticancer Activity Concentration Test in Mice

The geological activation crude extract of Example 1 was administered to females and males of healthy mice to observe changes in appearance or histopathological organs occurring within 24 hours (Toxicology Laboratory, Korea Chemical Research Institute, Korea Chemical Research Institute). Laboratory Toxicity Assay, pp 125-128, 1996).

As a result of observation of changes in histological or histopathological organs occurring within 24 hours by administering the general herbal test concentrations of 1g, 500mg and 250mg / kg, the 250mg dose did not interfere with survival. Histopathological observations showed no problems. As a result, the anticancer activity test was carried out at concentrations of 250 and 125 mg / kg (see Table 2).

Dosage Survival rate (death / test subject) Geologically active crude extract 1g 5/5 Geologically active crude extract 500mg 5/5 Geologically active crude extract 250mg 0/5 Geologically active crude extract 125mg 0/5

Experimental Example 3. Test of the inhibition effect of solid cancer cell line in mouse model of geological activation extract

The following experiment was performed to investigate the inhibitory effect of solid cancer in the real animal model of geological activity extract.

Animal models were composed of 4 groups of 5 to each group using 3 to 4 week old C57bl / 6 male mice (13-17 g, Korea Research Institute of Bioscience and Biotechnology). After one week of adaptation, the back hairs were removed to expose the skin with an area of 2 × 2 cm ² , and the mouse passage of Lewis lung carcinoma (ATCC, USA) cell passage as a cancer initiator was approximately 10,000. The cancer cells of dogs / ml were suspended and transplanted into the left inguinal part of healthy mice by 0.1ml each to induce solid cancer.The negative control group was untreated group with 1ml of physiological saline and the positive control group was anticancer agent. Adriamycin 3mg / kg, experimental group was treated with geological activation crude extract 125mg / kg, 250mg / kg, respectively.

Tumors were measured by measuring the length of the long and short radius of the tumor and calculating the volume of the tumor at intervals of 3 days from the 10th day from the day of significant occurrence. The decision was made on the 28th day after transplanting the cancer cells, the mice were killed and the resulting solid cancer was taken out and weighed to obtain the average tumor weight to calculate the percentage of the percentage of mass-sustaining inhibition (Yatsunami, J., Int. J. Oncol. , 17, pp 1151-1156, 2000).

As a result of confirming the inhibitory effect of GRI-activated crude extracts on solid tumors through the mouse model that induced tumors with Lewis lung lung epithelial cancer cells, Adriamycin, an anticancer drug treated with positive control, showed 92.1% inhibition effect. It was confirmed that 250mg / kg showed a solid cancer suppression effect of 52.1% at 84.9%, 125mg / kg (see Fig. 2 and Table 3).

Dosage Solid cancer inhibition rate (%) Untreated group (physiological saline) 0 Geological Activity Methanol Extract 125mg 52.1 Geological Activity Methanol Extract 250mg 84.9 Anticancer drug (campotetin) 92.1

Experimental Example 4. Toxicity Test of Geological Activity Extract

Animal experiments were conducted to confirm the toxicity of the geologically active crude extracts, polar and nonpolar solvent soluble extracts of Examples 1 and 2.

ICR-based BALB mice (Korea Institute of Bioscience and Biotechnology Genetic Genetic Center) 20 to 25 g each of 10 males divided into 4 groups and then the geological activation crude extracts of the present invention were dosed at 125, 250, 500 and 1000 mg / kg, respectively. Oral administration. After oral administration, toxicity was observed for 2 weeks, and none of the 125 and 250 mg / kg groups died. All 500 and 1000 mg / kg groups died. The autopsy revealed that histologically no symptoms were found.

The extract of geographic activity of the present invention can be administered in the following formulations, the formulation examples below are merely to illustrate the invention, whereby the content of the invention is not limited.

1. Preparation of powder

Chloroform Soluble Extract of Geological Activity 100mg

Corn Starch 100mg

Lactose 100mg

Talc 10mg

The above ingredients are mixed and filled in airtight cloth to prepare a powder.

2. Preparation of Tablets

Chloroform Soluble Extract of Geological Activity 100mg

Corn Starch 100mg

Lactose 100mg

2 mg magnesium stearate

After mixing the above components and tableting according to the manufacturing method of the conventional tablet to prepare a tablet.

3. Preparation of Capsule

Chloroform Soluble Extract of Geological Activity 100mg

Lactose 50mg

1 mg magnesium stearate

The above ingredients are mixed and compressed into tablets according to a conventional method for preparing capsules to fill gelatin capsules.

4. Manufacture of liquid

Chloroform Soluble Extract of Geological Activity 100mg

10 g of isomerized sugar

10g per book

Lemon flavor

Purified water

According to the conventional method for preparing a liquid solution, each component is added to the purified water, dissolved, and lemon flavor is added, and then purified water is added to adjust the total amount to 100 ml, and then filled into a brown bottle to prepare a liquid solution.

Formulation Example 5 Preparation of Healthy Food

Chloroform Soluble Extract of Geological Activity1000 mg

Vitamin mixture

Vitamin A Acetate70 ㎍

Vitamin E1.0 mg

Vitamin B ₁ 0.13 mg

Vitamin B ₂ 0.15 mg

Vitamin B ₆ 0.5 mg

0.2 μg of vitamin B ₁₂

Vitamin C10 mg

Biotin 10 ㎍

Nicotinic Acid Amide1.7 mg

Folic acid 50 ㎍

Calcium Pantothenate 0.5 mg

Mineral mixture

Ferrous Sulfate1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate25.3 mg

Potassium phosphate monobasic 15 mg

Dibasic calcium phosphate55 mg

Potassium Citrate 90 mg

Calcium Carbonate 100 mg

Magnesium chloride24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixtures is mixed with a component suitable for a health food in a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health food manufacturing method. The granules may be prepared and used for preparing a health food composition according to a conventional method.

Formulation Example 6 Preparation of Healthy Drink

Chloroform Soluble Extract of Geological Activity1000 mg

Revitalization extract 1000 mg

Citric Acid1000 mg

Oligosaccharides 100 g

Plum concentrate 2 g

1 g of taurine

Add 900 ml of purified water

After mixing the above components in accordance with a conventional healthy beverage production method, and stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed sterilization and then refrigerated and stored in the present invention For the preparation of healthy beverage compositions.

Although the composition ratio is a composition suitable for a preferred beverage in a preferred embodiment, the composition ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, use purpose.

As described above, the present invention can provide a functional food having anticancer effect by preparing various foods and beverages containing gelatin active extract.

Geologically active crude extract, polar and nonpolar solvent soluble extract of the present invention inhibits the growth of cancer cells in vitro and significantly inhibits solid cancer induced in animal models, and thus can be used as a useful medicine for the treatment and prevention of various cancer diseases. .

Claims (14)

Crude extract of Angelica purpuraefolia Chung, showing the prevention and treatment of cancer diseases. The extract of claim 1 wherein the crude extract is soluble in a polar solvent selected from water, methanol, butanol or a mixed solvent thereof. Polar solvent soluble extract of geographic activity that shows the effect of preventing and treating cancer diseases. The extract of claim 3 wherein the polar solvent is soluble in a solvent selected from water, methanol, butanol or a mixed solvent thereof. Nonpolar solvent soluble extract of geographic activity that shows the effect of preventing and treating cancer diseases. 6. The extract of claim 5, wherein the nonpolar solvent is soluble in a solvent selected from hexane, chloroform, ethyl acetate. A pharmaceutical composition for the treatment and prevention of cancer diseases comprising a geographic activity crude extract as an active ingredient, and a pharmaceutically acceptable carrier. Geologically active polar or nonpolar solvent soluble extract as an active ingredient, a pharmaceutical composition for the treatment and prevention of cancer diseases comprising a pharmaceutically acceptable carrier. The pharmaceutical composition according to claim 7 or 8, wherein the daily dosage of the composition is 125 to 250 mg / kg body weight. According to claim 7 or 8, wherein the cancer disease is cancer of the lung, bone cancer, pancreatic cancer, skin cancer, blood cancer, head or neck cancer, skin cancer, uterine cancer, ovarian cancer, rectal cancer, stomach cancer, colon cancer, breast cancer, Pharmaceutical composition comprising vulvar carcinoma, esophageal cancer, small intestine cancer or one or more combinations of these cancers. A cancer treatment adjuvant having a cancer cell development-inducing activity comprising a geographic activity crude extract, a polar or non-polar solvent soluble extract as an active ingredient. A health supplement comprising a crude extract of geological activity and a food acceptable additive to support the cancer disease. A dietary supplement comprising a polar or non-polar solvent soluble extract of geological activity that exhibits a preventive effect of cancer diseases and a food acceptable additive. The health supplement according to claim 12 or 13, comprising Angelica koreana MAX extract.