KR20040035336A - A concentration-liquid production method and food by using of the deer-meat and deer-bone - Google Patents

Abstract

PURPOSE: A production of making an extract containing ganglioside from venison and its bone without the antler tips of a deer is provided. The extract is made into the shape of soft gelatin capsules, hard gelatin capsules, granules, tablets, pills and drinks. CONSTITUTION: Venison as the flesh of a deer and its bone are extracted to give an extract containing ganglioside. To make a test liquid, venison and bone are added with a Folch solution, homogenized for 10min, shaken with distilled water and filtered. Then, the filtrate is concentrated in a vacuum evaporator, added with a minimum amount of a solvent and stored in a frozen state to give a sample. The sample and a standard liquid are used to measure the amount of ganglioside contained in the flesh and bone of the deer.

Description

A concentration-liquid production method and food by using of the deer-meat and deer-bone}

The present invention extracts the ganglioside (Ganglioside) from the stag bone and produce it as a concentrate, and extract the gangloside from the deer meat (and stag bone) to make it as a powder to be used as food foods and bones It relates to a method for producing a concentrate using and foods using the same.

In general, the utility value of deer is mostly used as antler, which is a representative health food material, and deer meat is partially used, but there is no development of products using bones and by-products. to be.

Normally, antlers are freshly grown horns of bucks and processed and dried. Deer antlers start to drop off old horns and sprout new horns from mid-March each year. This is called.

Deer antler can be divided into manok, plum, siberian, and new zealand, and reindeer according to the mountain and deer. It is a noble herbal medicine widely used as the best blood tonic in oriental medicine.

Recently, animal experiments and clinical trials using deer antler have been found to have growth-promoting effects, aging inhibitory effects, hematopoietic function, heart function improvement, anti-muscular fatigue effect and anti- analgesic effect in children.

Hypoxanthine, a kind of nucleic acid metabolite known to be one of the aging inhibitors in antler, inhibits the activity of monoamineoxidase B, whose activity increases with aging as a result of animal experiments. It is known to act to inhibit aging.

In addition, Ganglioside, a type of glycolipid among the fat-soluble components of antler, is a glycolipid of polymers accumulated in the gray matter of kidney, liver, spleen, and brain. It is known to be valid.

As can be seen from the above animal and clinical test results, antler is an animal herbal medicine of high value as a medicine and a therapeutic agent containing a large amount of various ingredients effective for the human body, and contains a substance called gangliosides, and thus many people wanting health food. People are seeking antler, but there is a limit in supplying such antler and the antler is expensive and is not widely supplied to the general public.

The gangliosides have been recognized as a defense against infection of breast milk and milk products using such gangliosides have been produced by milk product companies.

However, the gangliosides included in the deer's horns are generally recognized to be included only in the deer's horns, so it is difficult to obtain expensive antlers for the general public, and thus they are not widely used.

The present invention is to solve the above conventional problems, the object of the present invention, in order to improve the drawbacks of the above-described conventional or conventional techniques, extract ganglioside from deer meat and bone in a short time as a result of long-term research The gangliosides from the deer bone to produce a concentrate, and the gangliosides from the deer meat (and stag bone) to produce a powder to be used as food to produce a concentrate using deer meat and bone and To provide food.

That is, the present invention does not extract the gangliosides recognized as contained only in the antler, but extracts from the deer antler or bone and extracts from the deer meat or bone to develop them as food and provide them to the public. To provide used food.

Hereinafter, an embodiment of the present invention will be described in detail with reference to the accompanying drawings.

Deer antler is a representative herbal medicine of rejuvenation and soy sauce, and ganglioside is an indicator of such antler quality and is known to have a high correlation with its content.

Traditional soups made with meat have the potential to serve as a new foodservice industry in our country's unique diet, which can be a subject of considerable interest.

Prepare the sample solution by dividing the deer meat and bone into parts and crushing 5g, add 200 ml of folch (CHC ?? ₃ : MeOH = 2: 1) solution, homogenize (10 minutes, 2 times) Filter into a funnel, add 20% (40 ml) of distilled water to the solvent, shake, and separate the layers for a long time.

Further, after separating the layers, the lower layer was collected and concentrated in a vacuum evaporator at 40 ° C., a minimum amount of solvent was added to dissolve the concentrated sample.

Meanwhile, 10 μl of the standard solution and each sample were added to Kieselgel 60F ₂₅₄ HPTLC-plate (10 cm × 10 cm) using LINOMAT to develop a developing solvent (CHCl 3: MeOH: Water = 65: 25: 4) developed by inserting a plate in a saturated chamber (chamber), evaporating the solvent to develop 10% sulfuric acid, heated in a 105 ℃ oven (20 minutes) Analyze using HPTLC Scanner.

In addition, a standard calibration curve was prepared from peak areas of Rf 0.42 and 0.44 obtained by dissolving MeOH in ganglioside standard by dissolving MeOH and performing experiment according to HPTLC method and scanning at wavelength of 360 nm with TLC scanner. do.

On the other hand, the sample solution of each sample was dissolved by adding MeOH, tested according to the HPTLC method, and ganglioside in the sample was quantified from the peak areas of Rf 0.42 and 0.44 obtained by scanning at a wavelength of 360 nm with a TLC scanner.

Of the ganglioside standards isolated from bovine brains, gangliosides representing Rf 0.42 and Rf 0.44 were used as standard products, and calibration curves were prepared in the area of 2 mg / ml using a TLC scanner. 957.7x + 162.5 (r = 0.9952), y = 1173.4x + 276.4 for Rf 0.44

(r = 0.9952).

When the sample is analyzed, quantification is possible in all samples, and the quantitative results are shown in Table 1 below.

The present invention, as described above, extract the ganglioside from the stag bone and stag meat, so that the ganglioside from the stag bone can be prepared as a concentrate and used as food, and the gangle from stag meat (and stag bone) Rioside furnace is made of powder so that it can be used as food.

In other words, when prepared with the concentrate can be easily carried while carrying, and when prepared with the powder can be eaten together with the stock solution such as food.

The form of preparation of the health food may be soft capsules, hard capsules, granules, tablets, pills and beverage products.

The present invention as described in detail above extracts gangliosides from deer bones and venison and extracts gangliosides, which were previously recognized to be included only in the deer's horns, by extracting gangliosides from deer bones and meat. After processing, there is an effect that can be provided as a food to be easily edible to the public.

Claims (3)

5 g of venison and bone are divided into parts and pulverized. Then, 200 ml of fold solution is added, homogenized twice for 10 minutes, filtered through a separatory funnel, and 20% (40 ml) of distilled water is added to the solvent. Separating first step: The second step of receiving the lower filtrate and concentrating in a vacuum evaporator at 40 ℃, adding a minimum amount of solvent to dissolve the concentrated sample, quantitated to 10ml and stored as a frozen sample. 10 μl each of the standard solution and the sample were added to the Kieselgel 60F ₂₅₄ HPTLC-plate by using Renomet, and then placed in a dipped plate in a chamber saturated with a developing solvent, followed by volatilization of the solvent and color development with 10% sulfuric acid. Third step of heating in oven for 20 minutes: The fourth step of preparing a calibration curve for the standard from peak areas of Rf 0.42 and 0.44 obtained by adding MeOH to the ganglioside standard by dissolving it and scanning at a wavelength of 360 nm with a TLC scanner: The sample solution of each sample was dissolved by adding MeOH, and tested according to the HPTLC method, followed by the fifth step of quantifying the gangliosides in the sample from the peak areas of Rf 0.42 and 0.44 obtained by scanning at a wavelength of 360 nm with a TLC scanner. Method for producing a concentrate using deer meat and bone characterized in that. The food using deer meat and bone according to claim 1, wherein the deer meat and the ganglioside extracted from the deer bone are made of powder. The food using deer meat and bone according to claim 2, wherein the ganglioside extracted from the deer bone is prepared as a concentrate.