KR20040018633A - The production method of assistant feed containing beet pulp and propylene glycol and thereof assistant feed - Google Patents

The production method of assistant feed containing beet pulp and propylene glycol and thereof assistant feed Download PDF

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KR20040018633A
KR20040018633A KR1020020050036A KR20020050036A KR20040018633A KR 20040018633 A KR20040018633 A KR 20040018633A KR 1020020050036 A KR1020020050036 A KR 1020020050036A KR 20020050036 A KR20020050036 A KR 20020050036A KR 20040018633 A KR20040018633 A KR 20040018633A
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propylene glycol
feed
beetroot
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test
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윤병현
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윤병현
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/111Aromatic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K30/00Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
    • A23K30/20Dehydration
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/174Vitamins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/10Feeding-stuffs specially adapted for particular animals for ruminants
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23NMACHINES OR APPARATUS FOR TREATING HARVESTED FRUIT, VEGETABLES OR FLOWER BULBS IN BULK, NOT OTHERWISE PROVIDED FOR; PEELING VEGETABLES OR FRUIT IN BULK; APPARATUS FOR PREPARING ANIMAL FEEDING- STUFFS
    • A23N17/00Apparatus specially adapted for preparing animal feeding-stuffs
    • A23N17/007Apparatus specially adapted for preparing animal feeding-stuffs for mixing feeding-stuff components

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  • Life Sciences & Earth Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
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  • Animal Husbandry (AREA)
  • Health & Medical Sciences (AREA)
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  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Physiology (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)

Abstract

PURPOSE: Provided are an assistant feed containing beet pulp and propylene glycol and production method thereof, thereby reducing ketone body in blood, urine, milk and the like of a ruminant suffering from ketosis. CONSTITUTION: A production method of an assistant feed is characterized by the steps of: drying 40.0-55.0 wt.% of beet pulp at 50-70 deg.C for 10-15 minutes and mixing with 45.0-60.0 wt.% of 99.5% liquid propylene glycol at 60-80 deg.C for 1.5-2.5 hours in a jacket-drier; and maturing the mixture for 30-40 minutes with decreasing the temperature from 60-80 deg.C to 15-40 deg.C.

Description

사탕무우박과 프로필렌글리콜을 함유한 보조사료의 제조방법 및 상기 방법으로 제조된 보조사료 조성물{THE PRODUCTION METHOD OF ASSISTANT FEED CONTAINING BEET PULP AND PROPYLENE GLYCOL AND THEREOF ASSISTANT FEED}TECHNICAL METHODS OF ASSISTANT FEED CONTAINING BEET PULP AND PROPYLENE GLYCOL AND THEREOF ASSISTANT FEED}

본 발명은 사탕무우박과 프로필렌글리콜을 함유한 보조사료의 제조방법 및 상기 방법으로 제조된 보조사료에 관한 것이다.The present invention relates to a method of preparing a feed supplement containing beetroot and propylene glycol and to a feed prepared by the above method.

일반적으로 포유류 소목에 속하는 반추동물은 되새김동물이라고도 하며, 영양붙이, 오카피, 기린과, 사슴과, 애기사슴과, 소과 동물 등이 속한다.In general, ruminants belonging to mammalian cattle are also called ruminants, and include antelope, okapi, giraffe, deer, baby deer, and bovine.

반추동물은 단위동물과는 달리 제1위(혹위:rumen),제2위(벌집위:reticulum), 제3위(겹주름위:omasum), 제4위(주름위, 진위:abomasum) 등의 다수개의 위를 가지고 있으며, 이중 제1위와 제2위를 반추위라고 한다. 반추위 내에는 여러종류의 미생물이 서식하고 있으며, 반추동물이 섭취한 사료는 반추위 내 미생물에 의해 분해된다. 반추위에서는 반추작용과 미생물에 의한 분해과정이 일어나며 제3위에서는 소량의 수분이 흡수되고 소화내용물을 제4위로 유출시키며 제4위는 단위 동물의 위와 비슷한 기능을 가지고 있어 진위라고도 하며 위산, 펩신, 레닌 등의 소화효소가 분비된다. 반추위 내 미생물은 섬유소 분해 박테리아, 전분박테리아, 암모니아 생성 박테리아 등 10여종에 달한다.Unlike ruminants, ruminants have a plurality of ruminants such as 1st place (rumen), 2nd place (reticulum), 3rd place (omasum) and 4th place (wrinkle, authenticity: abomasum) It has a stomach, of which the first and second positions are called rumen. There are many kinds of microorganisms in the rumen, and the feed consumed by the ruminants is broken down by the microorganisms in the rumen. In the rumen, rumination and microbial decomposition occur, and in the third place, a small amount of water is absorbed and the digestion contents are discharged to the fourth place. The fourth place has the same function as that of the unit animal. Digestive enzymes such as lenin are secreted. Rumen microorganisms include more than 10 species, including fibrinolytic bacteria, starch bacteria, and ammonia-producing bacteria.

반추동물은 반추위 내의 온도와 수분을 유지하고 미생물과 사료를 혼합하는 등 미생물의 생활여건을 조성해주며 반추위 내 미생물은 반추동물의 소화작용을 돕고 여러가지 영양소를 합성하여 반추동물이 이용할 수 있도록 한다. 반추위 내 미생물의 주요 기능을 살펴보면 섬유소(Cellulose, Hemicellulose)의 분해, 아미노산 합성, 비타민 B군과 K군의 합성, 미생물체 영양소의 공급 등을 들수 있다. 반추위 내 미생물의 기능 가운데 가장 중요한 것이 섬유소의 분해라고 할 수 있다. 반추동물이 섭취하는 사료의 약 75%는 탄수화물이며 목초와 같은 조사료에는 주로 셀룰로오스, 헤미셀룰로오스, 펙틴, 프락탄, 전분형태의 탄수화물이 포함되어 있다. 반추위 내 미생물은 이들 탄수화물을 분해하여 휘발성 지방산이라는 물질을 생성하여 반추동물의 주요 에너지원으로 사용하고 있다. 생산된 휘발성 지방산은 문맥을 통하여 반추위에서 75%가 흡수되고, 제3위와 제4위에서 약 19%, 소장에서 약 5%가 흡수된다.Ruminants maintain the temperature and water in the rumen and mix microorganisms with feed to create living conditions for the microbes. Ruminants help the ruminants to digest and synthesize various nutrients for use by ruminants. The main functions of microorganisms in the rumen include the decomposition of cellulose (Cellulose, Hemicellulose), amino acid synthesis, the synthesis of vitamin B and K groups, and the supply of microbial nutrients. The most important function of the microorganisms in the rumen is the breakdown of fiber. About 75% of the ruminant feed is carbohydrate, and forages such as grasses mainly contain carbohydrates in the form of cellulose, hemicellulose, pectin, fructan, and starch. The rumen microorganisms break down these carbohydrates to produce volatile fatty acids and use them as the main energy source for ruminants. The volatile fatty acids produced are absorbed 75% in the rumen through the portal vein, about 19% in the third and fourth positions and about 5% in the small intestine.

상기와 같은 소화기능을 가진 반추동물은 주로 비유초기에 많은 영양분을 필요로 하여 다음과 같은 이유로 케토시스가 발생한다.Ruminants having such a digestive function require a lot of nutrients in the early stages of non-infancy, causing ketosis for the following reasons.

첫째, 탄수화물을 주성분으로 하는 전분이나 당류를 많이 함유하고 있는 곡류사료를 다량으로 급여하면 반추위에서 발효가 너무 신속하게 진행되므로 우선 반추위 내의 pH가 사료 섭취 후 급격하게 저하되어 제1위내 이상발효에 의해 제1위 점막에서 많은 케톤체가 생성되어 케토시스를 유발한다. 또한 분만전·후 급격한 사료변화로 제1위 내 미생물 총의 이상변화, 제1위 이완증, 제4위 이완과 전위로 케토시스가 발생한다.First, when a large amount of starch or sugar-containing cereals containing carbohydrates is fed in a large amount, fermentation proceeds so quickly in the rumen that the pH in the rumen rapidly decreases after feeding, resulting in abnormal fermentation in the first stomach. Many ketone bodies are produced in the first gastric mucosa, causing ketosis. In addition, rapid changes in feed before and after delivery result in ketosis due to abnormal changes in the total microorganisms in the first place, first place relaxation, and fourth place relaxation and dislocation.

둘째, 반추위 동물 중에서 소의 간은 중간대사에 있어 중심적인 부분을 차지하고 있는데, 소화관에서 흡수된 모든 영양분은 간을 통과해서 대부분은 다른 중간대사물로 변하기도 하고, 소비되거나 축적된다. 비유에 필요한 혈중의 당(glucose)의 대부분은 당 이외의 물질로부터 당신생(Gluconeogenesis)에 의해 간에서 합성된다. 비유를 위해 동원된 지방산은 간세포에서 대사를 하나, 만일 대사부전이 되게 되면 간의 지방변성이 되게 된다. 간세포에 지방축적으로 인해 간기능의 부전을 초래하여 에너지 대사에 지장을 주어 최종적으로는 케토시스를 일으키게 된다. 따라서 케토시스는 당질의 부족을 수반하는 지방산의 대사장애에 의해 체내에 케톤체(aectoacetic acid, β-hydrokin 낙산, 아세톤의 총칭)가 이상적으로 증가하여 경련, 마비 등의 임상증상을 발현한 상태를 말한다. 원래 케톤체는 반추가축에 있어서 가장 중요한 에너지원이며, 생체내에 케톤체가 증가하여도 임상증상을 수반하지 않으면 케토시스라고 말하지 않는다.Secondly, among ruminants, cattle's liver is a central part of intermediate metabolism. All of the nutrients absorbed from the digestive tract pass through the liver, most of which are converted to other intermediate metabolites and consumed or accumulated. Most of the glucose in the blood needed for parables is synthesized in the liver by Gluconeogenesis from substances other than sugars. Fatty acids mobilized for parables metabolize hepatocytes, but if they become metabolized, they become fatty degeneration of the liver. Fat accumulation in hepatocytes results in failure of liver function, disrupting energy metabolism, and eventually causing ketosis. Therefore, ketosis refers to a condition in which ketone bodies (aectoacetic acid, β-hydrokin butyric acid, and acetone collectively) are ideally increased in the body by metabolic disorders of fatty acids accompanied with a deficiency of sugars, thereby causing clinical symptoms such as convulsions and paralysis. Originally, ketone bodies are the most important energy source for the ruminant axis, and they are not referred to as ketosis unless they are accompanied by clinical symptoms even if the ketone bodies increase in vivo.

현재 전세계적으로 반추동물의 제1위인 반추위 내용물의 이상발효와 간세포내 지방의 축적 등으로 특히 비유초기(분만 후 1∼2주)에 케토시스가 빈번히 발생하고 있다.At present, ketosis frequently occurs in the early lactation period (1 to 2 weeks after delivery) due to abnormal fermentation of ruminant contents, which are the first place of ruminants, and accumulation of fat in hepatocytes.

그러나 이러한 케토시스를 미연에 방지하기 위한 사료의 개발 및 탄수화물을 주성분으로 하는 사료 등의 품질저하를 방지하거나 효용을 증가시키는 보조사료의 개발은 미진한 편이다.However, the development of feed to prevent such ketosis and the development of supplementary feed to prevent the degradation or increase the utility of carbohydrate-based feed, etc. is insufficient.

상기 전술한 문제점을 해결하기 위한 본 발명의 목적은 사탕무우박과 프로필렌글리콜을 혼합하고 건조시켜 제조하여 사탕무우박을 함유한 보조사료 제조방법 및 상기 방법으로 생산된 보조사료를 제공하는데 특징이 있다.An object of the present invention for solving the above-described problems is characterized by providing a method of manufacturing a feed supplement containing beetroot and beets produced by mixing and drying beetroot and propylene glycol.

또한 본 발명은 상기 사탕무우박과 프로필렌글리콜에 나이아신을 더 포함하는 것을 특징으로 하는 사탕무우박을 함유한 보조사료 제조방법 및 상기 방법으로 생산된 보조사료를 제공하는데 특징이 있다.In another aspect, the present invention is characterized in that it provides a supplemental feed production method containing beetroot and the auxiliary feed produced by the method characterized in that it further comprises niacin in the beet and propylene glycol.

도 1은 본 발명에 따른 사탕무우박과 프로필렌글리콜을 함유한 보조사료의 제조공정도이다.1 is a manufacturing process of the supplementary feed containing beet hail and propylene glycol according to the present invention.

도 2는 본 발명에 사용된 리본형 프로펠러 건조기(Jacket-Dryer)의 외부도이다.2 is an external view of a ribbon propeller dryer (Jacket-Dryer) used in the present invention.

도 3은 본 발명에 사용된 리본형 프로펠러 건조기(Jacket-Dryer)의 내부도이다.3 is an internal view of a ribbon propeller dryer (Jacket-Dryer) used in the present invention.

상기 전술한 기술적 과제를 해결하기 위한 본 발명은 사탕무우박과 프로필렌글리콜을 혼합하고 건조시켜 제조한 사탕무우박과 프로필렌글리콜을 함유한 보조사료의 제조방법 및 상기 방법으로 제조된 보조사료에 관한 것이다.The present invention for solving the above-described technical problem relates to a method of preparing a feed supplement containing beet propylene and propylene glycol prepared by mixing and drying beetroot and propylene glycol and the auxiliary feed prepared by the above method.

이하 본 발명의 사탕무우박과 프로필렌글리콜을 함유한 보조사료의 제조방법을 도 1에 나타낸 바와 같이 제조공정별로 설명하면 다음과 같다.Hereinafter, the manufacturing method of the auxiliary feed containing the beetroot and propylene glycol of the present invention will be described by the manufacturing process as shown in FIG.

제1공정: 사탕무우박의 건조공정Step 1: drying the beetroot

사탕무우박(Beet pulp) 40.0∼55.0중량%를 도 2 또는 도 3에 나타낸 리본형 프로펠러 건조기(Jacket-Dryer)에 넣고 건조기의 내부온도 50∼70℃에서 10∼15분간 건조시킨다.40.0-55.0 weight% of beet pulp is put into the ribbon-type propeller drier shown by FIG. 2 or FIG. 3, and it dries for 10 to 15 minutes at 50-70 degreeC internal temperature of a drier.

제2공정: 사탕무우박과 프로필렌글리콜의 혼합공정Second Step: Mixing Beet Hail with Propylene Glycol

상기 제1공정에 의해 건조된 사탕무우박과 99.5% 용액 프로필렌글리콜 45.0∼60.0중량%를 60∼80℃에서 1시간 30분∼2시간 30분간 혼합교반하며, 이 과정에서 사탕무우박과 99.5% 용액 프로필렌글리콜 혼합물의 습기가 제거되면서 건조기의 프로펠러에 의해 부드러운 소립자가 된다.The beetroot and 99.5% solution propylene glycol 45.0 to 60.0% by weight of the first step were mixed and stirred at 60 to 80 ° C. for 1 hour 30 minutes to 2 hours and 30 minutes. As the moisture in the glycol mixture is removed, the propeller in the dryer makes it soft particles.

제3공정: 숙성공정Third Process: Aging Process

상기 제2공정에 의한 소립자를 건조기의 내부온도를 60∼80℃에서 15∼40℃로 감소시키면서 30∼40분간 숙성시킨다. 이 공정에서 나이아신(niacin)을 더 첨가할 수도 있다.The small particles obtained by the second step are aged for 30 to 40 minutes while the internal temperature of the dryer is reduced from 60 to 80 ° C to 15 to 40 ° C. Niacin may be added in this process.

또한 사탕무우박과 99.5% 용액 프로필렌글리콜과 나이아신을 함유한 보조사료의 제조방법은 사탕무우박 43.0∼45.0중량%, 프로필렌글리콜 52.0∼56.0중량%의 혼합물의 소립자의 숙성단계에서 나이아신 1.0∼3.0중량%를 첨가하는 것외에는 사탕무우박과 프로필렌글리콜을 함유한 보조사료의 제조방법과 동일하게 수행되었다.In addition, the method of preparing auxiliary feed containing beetroot, 99.5% solution of propylene glycol and niacin was 1.0-3.0% by weight of niacin in the ripening step of the small particles of a mixture of beetroot 43.0-45.0% by weight, propylene glycol 52.0-56.0% by weight. Aside from the addition, it was carried out in the same manner as in the preparation of the supplementary feed containing beetroot and propylene glycol.

이하 실시예 및 비교예를 통하여 본 발명을 더욱 상세히 설명하기로 한다. 이들 실시예, 비교예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로, 본 발명의 범위가 이들 실시예, 비교예에 국한되지 않는다는 것은 당업계에서 통상의 지식을 가진 자에게 있어서 자명할 것이다.Hereinafter, the present invention will be described in more detail with reference to Examples and Comparative Examples. These Examples and Comparative Examples are only for illustrating the present invention more specifically, and it will be apparent to those skilled in the art that the scope of the present invention is not limited to these Examples and Comparative Examples.

실시예 1Example 1

사탕무우박(Beet pulp) 40.0중량%를 리본형 프로펠러 건조기(Jacket-Dryer)에 넣고 건조기의 내부온도 60℃에서 10분간 건조하여 건조된 사탕무우박과 99.5% 용액 프로필렌글리콜 60.0중량%를 70℃에서 2시간 30분간 혼합교반하며, 이 과정에서 사탕무우박과 프로필렌글리콜 혼합물의 습기가 제거되면서 부드러운 소립자가 된다. 상기 소립자를 건조기의 내부온도가 70℃에서 30℃가 될 때까지 30분간 숙성시킨다.Beet pulp 40.0% by weight in a ribbon-type propeller dryer (Jacket-Dryer) and dried for 10 minutes at an internal temperature of 60 ℃ dried beetroot and 99.5% solution propylene glycol 60.0% by weight at 70 ℃ 2 The mixture is stirred for 30 minutes and, in the process, the moisture of the beetroot and propylene glycol mixture is removed to become soft small particles. The small particles are aged for 30 minutes until the internal temperature of the dryer is 70 ℃ to 30 ℃.

실시예 2Example 2

상기 사탕무우박 45.0중량%, 99.5% 용액 프로필렌글리콜 55.0중량%를 혼합하는 것 외에 실시예 1과 모두 동일하게 수행하였다.45.0% by weight of the beetroot, 99.5% solution was carried out in the same manner as in Example 1 except for mixing 55.0% by weight of propylene glycol.

실시예 3Example 3

상기 사탕무우박 50.0중량%, 99.5% 용액 프로필렌글리콜 50.0중량%를 혼합하는 것 외에 실시예 1과 모두 동일하게 수행하였다.50.0% by weight of the beetroot, 59.5% by weight of 99.5% solution propylene glycol was carried out in the same manner as in Example 1.

실시예 4Example 4

상기 사탕무우박 55.0중량%, 99.5% 용액 프로필렌글리콜 45.0중량%를 혼합하는 것 외에 실시예 1과 모두 동일하게 수행하였다.Except for mixing 55.0% by weight of the beetroot, 45.0% by weight of 99.5% solution propylene glycol was carried out in the same manner as in Example 1.

실시예 5Example 5

상기 사탕무우박 43.0중량%, 99.5% 용액 프로필렌글리콜 56.0중량%를 혼합하여 숙성단계에서 나이아신 1.0중량%를 첨가하는 것 외에 실시예 1과 모두 동일하게 수행하였다.The beetroot was mixed with 43.0% by weight, 99.5% solution propylene glycol 56.0% by weight, except that 1.0% by weight of niacin in the aging step was performed in the same manner as in Example 1.

실시예 6Example 6

상기 사탕무우박 44.0중량%, 99.5% 용액 프로필렌글리콜 54.0중량%를 혼합하여 숙성단계에서 나이아신 2.0중량%를 첨가하는 것 외에 실시예 1과 모두 동일하게 수행하였다.44.0% by weight of the beetroot, 54.0% by weight of 99.5% solution propylene glycol was mixed in the same manner as in Example 1 except adding 2.0% by weight of niacin in the aging step.

실시예 7Example 7

상기 사탕무우박 45.0중량%, 99.5% 용액 프로필렌글리콜 52.0중량%를 혼합하여 숙성단계에서 나이아신 3.0중량%를 첨가하는 것 외에 실시예 1과 모두 동일하게 수행하였다.45.0 wt% of the beetroot, 52.0 wt% of 99.5% solution propylene glycol was mixed and the same as in Example 1 except for the addition of 3.0% by weight of niacin in the aging step.

상기 실시예 5, 6, 7에 나이아신을 첨가함은 제1위내 미생물체 단백질의 합성량을 증가시켜 산유량 및 유단백 함량을 높이고, 특히 비유초기(분만 1∼2주)에 에너지 대사에 작용하여 체지방의 과도한 분해를 억제시키고 혈액내 포도당 수준을 높여 케토시스를 치료하여 산유량을 증가시키기 위함이다.The addition of niacin to Examples 5, 6, and 7 increases the amount of microorganism protein in the first stomach to increase acid yield and milk protein content, and in particular, in metabolic phase (1 to 2 weeks) to act on energy metabolism, This is to suppress excessive degradation and to increase blood glucose level by treating ketosis by increasing blood glucose level.

본 발명자는 상기 실시예 5에 의해 제조된 보조사료를 과학기술분석센타에 의뢰하여 조단백질 3.97%, 조섬유 7.52%, 조회분 3.07%, 프로필렌글리콜 55.69%라는 시험(검사)성적서를 받았다.The present inventors commissioned the auxiliary feed prepared according to Example 5 to the Science and Technology Analysis Center, and received a test (test) report of 3.97% crude protein, 7.52% crude fiber, 3.07% crude ash, and 55.69% propylene glycol.

시험예 1Test Example 1

상기 실시예 2의 방법으로 제조된 사탕무우박과 프로필렌글리콜을 함유한 보조사료를 제조하여 케토시스 증상이 있는 젖소 20마리를 각각 시험구 및 대조구로 하여, 상기 시험구에 시중에서 판매하는 사료((주)제일사료)에 본 발명을 1일 500g씩 첨가하여 8주간 급여하였고, 상기 대조구에는 사료((주)제일사료)만을 급여하여본 발명이 케토시스에 미치는 정도를 측정하였다.Supplementary feeds containing beetroot and propylene glycol prepared by the method of Example 2 were prepared, and 20 cows with ketosis symptoms were used as test and control, respectively. First feed) was added to the present invention 500g per day for 8 weeks, the control was fed only feed (primary feed Co.) was measured the extent of the present invention to ketosis.

일반적으로 케톤체가 축적되는 혈액, 뇨, 젖 중에 함유된 케톤체의 함량을 측정하였다.In general, the contents of ketone bodies contained in blood, urine, and milk where ketone bodies accumulate were measured.

본 시험예의 시험구 결과를 표 1에 나타내었고, 대조구 결과를 표 2에 나타내었다.The test result of this test example is shown in Table 1, and the control result is shown in Table 2.

본 발명을 함유한 사료를 급여한 시험구의 혈액, 뇨, 젖 내 케톤체의 양(㎎/㎗)Amount of ketone bodies in blood, urine, and milk of the test group fed the feed containing the present invention (mg / dl) 혈액blood Urinary milk 아세톤Acetone 아세토아세틱산Acetoacetic acid BHBBHB 아세톤뇨Acetoneuria 아세토아세틱산Acetoacetic acid BHBBHB 아세톤유Acetone oil 아세토아세틱산Acetoacetic acid BHBBHB 1주1 week 18.218.2 6.16.1 31.631.6 25.625.6 36.936.9 29.029.0 18.218.2 2.32.3 9.99.9 2주2 weeks 17.917.9 5.95.9 28.628.6 23.923.9 32.132.1 28.128.1 15.915.9 2.32.3 8.38.3 3주3 weeks 12.412.4 4.14.1 26.926.9 22.022.0 27.027.0 23.723.7 13.213.2 2.02.0 8.08.0 4주4 Weeks 10.810.8 3.93.9 20.820.8 16.516.5 20.820.8 19.519.5 11.711.7 1.81.8 7.57.5 5주5 Weeks 6.76.7 2.12.1 17.117.1 13.313.3 15.715.7 16.916.9 7.47.4 1.01.0 7.17.1 6주6 Weeks 3.43.4 1.01.0 14.814.8 8.88.8 9.49.4 14.014.0 4.14.1 0.70.7 6.06.0 7주Week 7 1.91.9 0.50.5 13.013.0 4.64.6 6.76.7 11.511.5 2.62.6 0.30.3 5.55.5 8주8 Weeks 0.80.8 0.30.3 12.312.3 1.81.8 4.64.6 10.010.0 1.31.3 0.30.3 5.15.1 *아세톤: Acetone, 아세토아세틱산: Acetoacetic acidBHB: 베타-하이드록시부티릭산(β-hydroxybutyric acid)Acetone: Acetone, Acetoacetic acid: Acetoacetic acid BHB: Beta-hydroxybutyric acid

본발명을 함유한 사료를 급여하지 않은 대조구의 혈액, 뇨, 젖 내 케톤체의 양(㎎/㎗)Amount of ketone body in blood, urine and milk of control group that did not receive feed containing the present invention (mg / dl) 혈액blood Urinary milk 아세톤Acetone 아세토아세틱산Acetoacetic acid BHBBHB 아세톤뇨Acetoneuria 아세토아세틱산Acetoacetic acid BHBBHB 아세톤유Acetone oil 아세토아세틱산Acetoacetic acid BHBBHB 1주1 week 16.716.7 4.94.9 29.029.0 22.322.3 38.238.2 25.925.9 16.416.4 1.61.6 8.08.0 2주2 weeks 16.916.9 5.65.6 28.428.4 21.521.5 37.937.9 25.725.7 15.915.9 1.71.7 7.97.9 3주3 weeks 15.715.7 6.26.2 27.927.9 24.624.6 38.038.0 26.826.8 16.216.2 1.51.5 8.18.1 4주4 Weeks 17.617.6 5.85.8 28.128.1 23.923.9 39.439.4 27.027.0 16.716.7 1.81.8 8.08.0 5주5 Weeks 16.016.0 7.17.1 29.329.3 22.722.7 37.137.1 26.926.9 16.516.5 1.71.7 7.87.8 6주6 Weeks 18.418.4 6.36.3 28.728.7 23.823.8 38.838.8 28.128.1 17.217.2 1.61.6 7.77.7 7주Week 7 16.916.9 6.66.6 28.528.5 24.124.1 39.939.9 27.727.7 16.516.5 1.71.7 8.28.2 8주8 Weeks 17.017.0 6.46.4 29.429.4 24.424.4 39.039.0 28.528.5 17.417.4 1.81.8 8.38.3 *아세톤: Acetone, 아세토아세틱산: Acetoacetic acidBHB: 베타-하이드록시부티릭산(β-hydroxybutyric acid)Acetone: Acetone, Acetoacetic acid: Acetoacetic acid BHB: Beta-hydroxybutyric acid

표 1, 2에 나타낸 바와 같이 시험구의 케톤체(ketone body; acetone, acetoacetic acid, β-hydroxybutyric acid)함량은 대조구에 비해 크게 감소하였다. 정상 젖소의 혈중 아세톤의 함량은 0㎎/㎗이고, 아세토아세틱산의 함량은 0㎎/㎗이고, BHB의 함량은 10.7㎎/㎗이다. 본 발명을 함유한 사료를 급여한 시험구에서 상기 아세톤, 아세토아세틱산 및 BHB가 시간이 경과함에 따라 감소하였다. 8주째의 아세톤, 아세토아세틱산 및 BHB함량이 정상치는 아니었으나, 유의하게 감소하였으며 정상치에 근접하였다. 그러나 표 2에 나타낸 바와 같이 본 발명을 첨가하지 않은 사료를 급여한 대조구의 아세톤, 아세토아세틱산 및 BHB은 시간에 따라 비례하지는 않았으나 정상치 이상의 함량을 유지하였다.As shown in Tables 1 and 2, the ketone body (acetone, acetoacetic acid, β-hydroxybutyric acid) content of the test was significantly reduced compared to the control. Normal cow's blood acetone content is 0mg / dl, acetoacetic acid content is 0mg / dl, and BHB content is 10.7mg / dl. In the test diet fed the feed containing the present invention, the acetone, acetoacetic acid and BHB decreased with time. Acetone, acetoacetic acid and BHB contents at 8 weeks were not normal, but significantly decreased and approached normal. However, as shown in Table 2, the acetone, acetoacetic acid and BHB of the control group fed the feed without the present invention maintained a content above the normal value although not proportional to time.

또한 정상 뇨중 아세톤 뇨의 함량은 1.0㎎/㎗, 아세토아세틱산은 3.4㎎/㎗, BHB는 11.7㎎/㎗이다. 표 1에 나타낸 바와 같이 시험구의 아세톤 뇨, 아세토아세틱산, BHB는 시간이 경과함에 따라 유의하게 감소함을 나타내었다. 그러나 표 2에 나타낸 바와 같이 대조구의 경우 시간에 비례하여 증가하지는 않았으나, 시험 시작 1주와 시험 종결 8주를 비교해 보면 1주에 비해 8주째에 증가한 것으로 나타났다.In addition, the content of acetone urine in normal urine was 1.0 mg / dl, acetoacetic acid was 3.4 mg / dl, and BHB was 11.7 mg / dl. As shown in Table 1, acetone urine, acetoacetic acid, and BHB of the test plots were significantly decreased over time. However, as shown in Table 2, the control did not increase in proportion to time, but when compared with the 1 week of the start of the test and the 8 weeks of the end of the test it was found that the increase in the 8th week compared to the 1 week.

또한 정상 유중 아세톤유의 함량은 0㎎/㎗, 아세토아세틱산은 0㎎/㎗, BHB는 4.9㎎/㎗이다. 표 1에 나타낸 바와 같이 시험구의 아세톤유, 아세토아세틱산, BHB는 시간이 경과함에 따라 유의하게 감소함을 나타내었다. 그러나 표 2에 나타낸 바와 같이 대조구의 경우 시간에 비례하여 증가하지는 않았으나, 시험 시작 1주와 시험 종결 8주를 비교해 보면 1주에 비해 8주째에 증가한 것으로 나타났다.The content of acetone oil in normal oil is 0 mg / dl, acetoacetic acid is 0 mg / dl, and BHB is 4.9 mg / dl. As shown in Table 1, the acetone oil, acetoacetic acid, and BHB of the test plots were significantly decreased over time. However, as shown in Table 2, the control did not increase in proportion to time, but when compared with the 1 week of the start of the test and the 8 weeks of the end of the test it was found that the increase in the 8th week compared to the 1 week.

또한 본 시험예의 시험구는 케톤체 함량이 시간에 비례하여 감소하였으며, 이러한 결과는 본 발명이 탄수화물의 공급원으로 사탕무우박과 지방의 공급원으로 프로필렌글리콜을 균형있게 공급함으로써 케톤체가 감소한 것으로 유추된다.In addition, the test zone of this test example, the content of ketone body decreased in proportion to time, and this result is inferred that the present invention reduced the ketone body by supplying propylene glycol as a source of sugar beet and fat as a source of carbohydrates.

시험예 2Test Example 2

임신한 젖소 20마리를 각각 시험구, 대조구를 대상으로 분만 2주전, 분만 후 2주간, 분만 후 2∼6주간의 기간에 시중에 판매되는 사료((주)제일사료)에 본 발명을 1일 50g, 100g, 150g, 200g, 250g, 300g씩 첨가하여 8주간 급여하였고,첨가하여 상기 각 기간에 따른 산유량에 미치는 본 발명의 정도 및 적정량을 측정하였다.Twenty pregnant cows were tested on the test and control groups, respectively, for two weeks before delivery, two weeks after delivery, and two to six weeks after delivery. 50g, 100g, 150g, 200g, 250g, 300g each was added for 8 weeks, and was added to measure the degree and the appropriate amount of the present invention on the oil flow according to each period.

본 발명이 산유량에 미치는 영향Effect of the present invention on acid flow 50g50 g 100g100 g 150g150 g 200g200 g 250g250 g 300g300 g 분만2주전2 weeks before delivery 대조구Control 175.1175.1 180.4180.4 176.9176.9 188.6188.6 186.9186.9 187.0187.0 시험구Test 198.7198.7 216.9216.9 218.1218.1 226.7226.7 220.4220.4 214.9214.9 분만2주후2 weeks after delivery 대조구Control 215.3215.3 228.4228.4 223.8223.8 221.1221.1 224.7224.7 223.2223.2 시험구Test 217.9217.9 229.6229.6 235.7235.7 246.8246.8 243.4243.4 245.9245.9 분만2∼6주후2-6 weeks after delivery 대조구Control 227.4227.4 218.9218.9 221.3221.3 225.7225.7 227.9227.9 226.8226.8 시험구Test 236.4236.4 253.2253.2 259.7259.7 254.6254.6 249.1249.1 247.5247.5 * 산유량은 1주일 산유량의 평균량으로 나타냄.* The oil production rate is expressed as the average amount of oil production per week.

표 3에 나타낸 바와 같이 시험구의 산유량은 대조구에 비해서 유의하게 증가하였다. 젖소의 개별적 능력에 따라 차이가 있지만 통계상 비임신 젖소의 1주일 평균 산유량은 175∼210㎏으로 본 실시예에 의한 결과는 대조구에 비해 시험구의 산유량이 증가함을 나타내었다.As shown in Table 3, the yield of the test group was significantly increased compared to the control group. Although there is a difference depending on the cow's individual ability, the average weekly milk yield of non-pregnant cows is 175-210 kg, and the results of this example showed that the milk yield of the test was increased compared to the control.

또한 본 발명이 산유량을 증가시키는 적정량은 분만 2주전인 경우 본 발명 100∼200g을 일반사료에 첨가하여 일일 급여하였을 때 산유량이 증가하였고, 분만 2주후인 경우 본 발명 200∼300g을 일반사료에 첨가하여 일일 급여하였을 때 산유량이 증가하였고, 분만 2∼6주 후인 경우 본 발명 200∼300g을 일반사료에 첨가하여 일일 급여하였을 때 산유량이 증가하였다.In addition, the appropriate amount of the present invention to increase the amount of acid is increased 2 weeks before delivery when the daily feed by adding 100 ~ 200g of the present invention to the general feed, and when 2 weeks after delivery of the present invention 200 ~ 300g added to the general feed When the daily feed was increased, the milk yield increased, and when 2-6 weeks after delivery, 200-300 g of the present invention was added to the general feed when the daily feed increased.

상기와 같은 결과는 케토시스가 젖소의 체내에서 탄수화물대사와 지방대사 이상과 간기능 저하 등을 일으켜 케톤체가 축적되어 식욕부진, 체중감소, 산유량 감소 등의 증상이 나타나는데 본 발명은 탄수화물의 공급원으로 사탕무우박과 지방의 공급원으로 프로필렌글리콜이 적정한 비율로 함유되어 식욕과 체중을 증가시켜 산유량을 증가시키는 것으로 유추되었다.These results indicate that ketosis causes carbohydrate metabolism and fat metabolism abnormality and liver function deterioration in the cow's body, resulting in ketone body accumulation, resulting in loss of appetite, weight loss, and decreased milk yield. It has been inferred that propylene glycol is the proper source of excess fat, increasing appetite and body weight, increasing acid output.

본 발명은 상술한 특정의 실시예, 시험예, 도표 및 도면에 기재된 내용에 기술적 사상이 한정되지 아니하며, 청구범위에서 청구하는 본 발명의 요지를 벗어남이 없이 당해 발명이 속하는 기술분야에서 통상의 지식을 가진 자라면 누구든지 다양한 변형의 실시가 가능한 것은 물론이고, 그와 같은 변경은 청구범위 기재의 범위 내에 있게 된다.The present invention is not limited to the technical spirit described in the specific embodiments, test examples, diagrams and drawings described above, and the general knowledge in the art to which the invention belongs without departing from the spirit of the invention claimed in the claims. Of course, any person having a variety of modifications can be made, and such changes are within the scope of the claims.

상기에서 상세히 설명하고 입증한 바와 같이 본 발명은 사탕무우박과 프로필렌글리콜을 주성분으로 보조사료를 제조함으로써 충분한 탄수화물과 지방을 공급하여 영양적으로 균형을 이루므로 케톤체의 생성이 저하되는 효과가 있다.As described and demonstrated in detail above, the present invention has a nutritional balance by supplying sufficient carbohydrates and fats by preparing auxiliary feeds based on beetroot and propylene glycol, thereby reducing the production of ketone bodies.

또한 본 발명은 상기 케톤체의 생성을 저하시켜 식욕 및 체중을 증가시켜 젖소의 산유량을 증가시키는 효과가 있다.In addition, the present invention has the effect of reducing the production of the ketone body to increase the appetite and weight to increase the milk yield of cows.

Claims (3)

건조기에서 사탕무우박 40.0∼55.0중량%를 50∼70℃에서 10∼15분간 건조시켜 99.5%용액 프로필렌글리콜 45.0∼60.0중량%와 60∼80℃에서 1시간 30분∼2시간 30분간 혼합하여 습기가 제거된 후 사탕무우박과 프로필렌글리콜 혼합물의 소립자를 60∼80℃에서 15∼40℃가 되도록 30∼40분간 숙성시키는 것을 특징으로 하는 사탕무우박과 프로필렌글리콜을 함유한 보조사료의 제조방법.In the dryer, 40.0-55.0 wt% of beetroot was dried for 10-15 minutes at 50-70 ℃, and 45.0-60.0 wt% of 99.5% solution propylene glycol was mixed with 60-80 ℃ for 1 hour 30 minutes to 2 hours 30 minutes. After removing the small particles of the beet propylene and propylene glycol mixture for 30 to 40 minutes at 60 to 80 ℃ 15 to 40 ℃ characterized in that the production method of auxiliary feed containing beet propylene and propylene glycol. 제 1항에 있어서,The method of claim 1, 상기 사탕무우박과 99.5%용액 프로필렌글리콜 혼합물의 숙성단계에서 나이아신 1.0∼3.0중량%를 더 첨가하는 것을 특징으로 하는 사탕무우박과 프로필렌글리콜을 함유한 보조사료의 제조방법.Method of producing a supplementary feed containing beet propylene and propylene glycol, characterized in that further adding 1.0 to 3.0% by weight of niacin in the aging step of the beetroot and 99.5% solution propylene glycol mixture. 제 1항 또는 제 2항의 방법으로 생산된 사탕무우박과 프로필렌글리콜을 함유한 보조사료 조성물.A feed composition comprising beetroot and propylene glycol produced by the method of claim 1 or 2.
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US4371558A (en) * 1981-02-11 1983-02-01 Liggett Group Inc. Semi-moist dog food preparation
US4600586A (en) * 1983-08-08 1986-07-15 Green Milton L Livestock feed lot adaptation composition and method
KR950026379A (en) * 1994-03-23 1995-10-16 이삼우 Lysine protected against ruminant degradation of ruminant, its manufacturing method and animal feed containing same
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KR100832741B1 (en) * 2006-04-13 2008-06-09 (주)이엘티사이언스 The liquid energy composition of propylene glycol for animal and a Method thereof

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