KR20040001551A - An enzyme extracting method of skin extract including hesperidin and the skin extract thereby, an extracting method of mineral extract from chenopodiaceae and the mineral extract thereby, an composition including the skin extract and the mineral extract, and an using method of the skin extract, the mineral extract or the composition for food addition - Google Patents

Abstract

PURPOSE: Provided are an enzyme extracting method of fruit skin including hesperidin and a fruit skin extract thereby, an extracting method of mineral components from Salicornia Herbacea L. and a mineral extract thereby, a mixed composition including the fruit skin extract and the mineral extract, and a using method of the fruit skin extract, the mineral extract or the mixed composition as food additives. CONSTITUTION: An enzyme extracting method of fruit skin including hesperidin comprises the steps of: preparing citron skin and mixing it with saccharide; fermenting the mixture and filtering it; and obtaining a fruit skin extract from the filtrate. A extracting method of mineral components from Salicornia Herbacea L. comprises the steps of: preparing Salicornia Herbacea L. and making it to charcoal; re-heating the charcoal and pulverizing it; mixing the pulverized charcoal and water and precipitating them; and separating a clean water layer and evaporating it to give a mineral extract.

Description

Enzyme extraction method of the skin extract containing hesperidin, the skin extract extracted by the extraction method, the method of extracting mineral components from the seaweed and the minera extract obtained by the extraction method, the extract and the mineral extract TECHNICAL EXTRACTING METHOD OF SKIN EXTRACT INCLUDING HESPERIDIN AND THE SKIN EXTRACT THEREBY, AN EXTRACTING METHOD OF MINERAL EXTRACT FROM CHENOPODIAL ATRA AND THE THE THE MITH COMPOSITION INCLUDING THE SKIN EXTRACT AND THE MINERAL EXTRACT, AND AN USING METHOD OF THE SKIN EXTRACT, THE MINERAL EXTRACT OR THE COMPOSITION FOR FOOD ADDITION}

The present invention relates to a composition having a component effective for body fat decomposition and prevention of osteoporosis, and a method for preparing the composition, and more specifically, to a method for extracting active ingredients including hesperdine extracted from citrus peel and by the extraction method Method of extracting the mineral components from the extracted skin extract, seaweed and the mineral extract extracted by the extraction method, the compound composition made by combining the hesperdine-containing skin extract and the mineral extract in a certain compounding ratio and the skin extract, mineral extract Or it relates to a method of using the blend composition as a food additive.

With the rapid development and modernization of the industry, the modern people's living culture and environment are rapidly changing, and as the mental and physical activities increase, the stress increases, resulting in indigestion and incompatibility of the human body, Various types of adult diseases are appearing. In addition, due to the busy life as described above, as the dietary culture changes, the amount of exercise decreases and the intake of instant foods and high-fat foods increases, resulting in constipation and obesity. In other words, while traditional vegetarian-oriented dietary changes are starting to change due to economic development and the development of the food industry, consuming more fat and calories than necessary, the amount of exercise continues to decrease, and high blood pressure directly induced by high-fat diets. As circulatory disease increases, obesity is expanding into a whole social problem.

Therefore, in recent years, research and development has been made in various angles to solve the above problems, and as a result, Korean Patent Publication No. 1998-002707 discloses a cardiovascular system including citrus peel extract, hesperdine or naringin purified therefrom. A disease prevention and treatment composition is disclosed. The invention disclosed in Patent Publication No. 1998-002707 discloses a pharmacologically active substance from a material reported to have a cardiovascular prevention effect [Jean Carper, Food Pharmacy, Bontam Books (1989)]. Intensive extraction attempts resulted in significant reductions in blood cholesterol and triglycerides due to HMG-CoA reductase inhibitory activity of citrus, orange peel citrus extracts, and purified hesperidin and naringin. It can be made. However, when the hydrolysis method using ethanol is used to extract hesperdine from the citrus peel as in the invention disclosed in Korean Patent Publication No. 1998-002707, it is possible to exclude the extraction of other active ingredients contained in the citrus peel. There was a problem.

In addition, various health supplements for preventing and treating obesity, such as dietary supplement composition of Korean Patent Publication No. 180452 and dietary fiber-containing dietary composition of Korean Patent Publication No. 2001-0103065 have been known, but such health supplement Foods did not provide a complete solution to obesity.

Therefore, the above known techniques not only provide an extraction method that enhances the pharmacological effect of hesperdine, but also have not been used to directly inhibit hesperdine in obesity, but also prevent the osteoporosis by using the pharmacological effect of hesperdine. Since there was no technology for this at all, there was a high demand in the art for a new composition to solve various problems such as preventing the development of adult diseases caused by obesity, osteoporosis prevention.

The present invention provides an enzyme extraction method for more effectively extracting the active ingredient including hesperdine from citrus peel through a new method different from the invention disclosed in the Patent Publication No. 1998-002707 to solve the above problems It aims to do it.

The present invention provides a skin extract containing the active ingredients including hesperidin extracted by the above method.

Another object of the present invention is to provide a method for effectively extracting various mineral components in an ionic state that are easily absorbed into the body from seaweed.

Still another object of the present invention is to provide an ionic mineral extract extracted by the above method.

Still another object of the present invention is to provide a compound composition in which the pharmacological effect of the extracts is increased by combining the extract of hesperdine and the extract of minerals and mineral extracts by the above methods in a predetermined ratio.

It is also an object of the present invention to provide an extract or a combination composition that can be used as a food additive that lowers harmful cholesterol levels in the body and is effective in preventing and treating osteoporosis.

In order to achieve the object of the present invention as described above, the present invention comprises the steps of preparing a citrus peel; Combining the prepared citrus peel and sugar; Fermenting the blended citrus peel and sugar; Filtering the fermentation broth; And it provides a skin extract enzyme extraction method comprising the step of obtaining a skin extract from the filtrate.

The present invention also provides a method for extracting the skin extract enzyme comprising the step of preparing the citrus peels in the shade of the citrus peel and crushing the shaded citrus peel.

The present invention also provides a method for extracting the skin extract enzyme, characterized in that the step of combining the prepared citrus peel and sugar in a weight ratio of 1: 2-1: 10.

The present invention also provides a method for extracting the extract of fruit juice, wherein the sugar used in the step of combining the prepared citrus peel and sugar is fructose.

The present invention also provides a method for extracting the skin extract enzyme comprising the step of fermenting for 1-4 months at 15-25 ℃.

The present invention also provides a method for extracting the skin extract enzyme comprising the step of evaporating the filtrate in a water of 55-65 ℃ the step of obtaining the skin extract.

The present invention provides a skin extract prepared by any one of the above methods.

The present invention also provides a skin extract including the hesperidin, rutin, pectin, etc., wherein the extract is an effective ingredient for cholesterol degradation.

The present invention comprises the steps of preparing a seaweed; Making seaweed charcoal from the prepared seaweed; Reheating the seaweed charcoal; Pulverizing the reheat treated seaweed charcoal; combining the pulverized seaweed charcoal powder and water to make charcoal; Sinking the charcoal for a period of time to separate only the clear water above; And it provides a mineral extract extraction method comprising the step of obtaining the mineral extract by evaporating the separated water.

The present invention also provides a method for extracting the mineral extract, characterized in that the step of making the charcoal combines the pulverized seaweed charcoal powder and water in a weight ratio of 1: 5-1: 10.

The present invention also provides a mineral extract extracted by the extraction method.

The present invention also provides a mineral extract containing the Na, Ca, K, Mg, Zn, Fe, Cu, Ni, Mn.

The present invention provides a blended composition comprising the skin extract and the mineral extract in a weight ratio of 10: 1-10: 5.

The present invention also provides a blend composition wherein the blend composition comprises hesperdine, rutin, pectin and various mineral components.

In another aspect, the present invention provides a method for using the skin extract, mineral extracts, blended composition as a food additive.

A method of extracting mineral components from the extracts of the skin and the extract of the skin through the new enzyme extraction method that can be extracted from citrus peels according to the present invention from flaky nodule components present in the skin other than hesperdine and extracted by the extraction method A new blend composition comprising mineral extracts in a certain blending ratio and a method of using the extract or blend composition as a food additive, and explaining the function of these components in the extract or blend composition.

Hesperidin contained in the skin of citrus fruits extracted by the enzyme extraction method according to the present invention corresponds to vitamin P among water-soluble vitamins, which is provitamin, and in 1936, Gyorgy used only vitamin C to treat rat scurvy. Insufficient and lemon juice is more effective, so this active ingredient is called vitamin P. In other words, the deficiency of this vitamin increases the osmoticity of blood vessels, causing purple spots on the skin, which is called P in the sense of capillary permeability factor. Vitamin P is thus a factor that controls the osmoticity of capillaries and prevents bleeding with vitamin C. Vitamin P is a pale yellow water-soluble pigment that combines flavonoids with a disaccharide rutinose, a glycoside, and currently has the action of vitamin P. Hesperidin, eriodictin, rutin ) Is known. Vitamin P is widely distributed in leafy vegetables, especially citrus peels and buckwheat. The daily requirement for vitamin P is not clear, but about 40-50 mg is needed. This vitamin P is a water-soluble vitamin, soluble in water, and the excess is excreted in the urine. If the deficiency in the body immediately manifests symptoms of deficiency, you need to be fed every day to prevent illness.

Hamcho (meaning salty grass) grows in coastal wetland and absorbs seawater at high tide and receives sunlight at low tide, and the photosynthesis evaporates the moisture of leaves and stems, leaving only the active ingredients in the seawater. It has the physiology that it is, and it is characteristic that it retains all the ingredients in sea water even though it is six seconds. In other words, it contains various minerals except for the bitterness of seawater in the leaves and stems, and it can be said that the seaweed is ideally reducing the nutrients that were taken from the land to the sea. In addition, the seaweed contains various minerals and other necessary ingredients for the human body. In other words, the seaweed is an excellent sea food. In addition, the salt and fiber contained in the seaweed increases the peristalsis of the intestines, so it plays a role in discharging even the remaining stool in the intestine. The seaweed contains a large amount of minerals. Iron is 15 to 44 times more than sea horses, seaweed, seaweed, and kelp, which are said to have the highest iron content among seaweeds. In addition, as a result of long experiments, the seaweed is expected to contain all 94 kinds of minerals dissolved in seawater. It contains a very small amount of various elements that cannot be detected by current analytical techniques, and modern science has not yet revealed the nutritional or biochemical role of these elements in the human body. Unlike seaweeds such as kelp and seaweed that grow in seawater, seaweed absorbs and grows in seawater, but is a plant that performs photosynthesis by directing sunlight and air on the ground. Therefore, it is assumed that the seaweed has a special physiology, composition, and many biochemical secrets that are different from algae that grow in the sea and other plants that grow in the sea. So far, the ingredients of seagrass and its biochemical mechanisms cannot explain these experimental results. For this reason, we can only think that there is a huge biochemical secret hidden in Hamcho.

Hamcho is also the heaviest grass on earth. This is also a characteristic not found in other plants. The most noticeable effect on the effect of seaweed on the human body is the action of enzymes and minerals contained in the seaweed. Since the enzymes in the seaweed are enzymes that break down fats and proteins, they not only break down the stool in the small intestine, a triglyceride, but also break down the body's blood, blood vessels, and unnecessary fat that is deposited in each organ. In addition, the rich minerals contained in the seaweed quickly eliminate the health obstacles that modern people have to eat mineral-deficient food due to chemical farming methods.

As described above, the present invention provides the fact that the skin extract obtained using the new enzyme extraction method, which is not known at all, is more effective than the conventional hesperdine and the mineral extraction method of the present invention, ie, seaweed, for body fat decomposition, especially cholesterol decomposition and osteoporosis prevention. It is based on an extraction method for extracting various mineral components which are extremely easy to absorb in the body. In addition, the present invention in the osmotic action in the process of controlling the osmoticity of the capillaries in the function of hesperidin known as vitamin P extracted by the enzyme fermentation in citrus peel by the enzyme extraction method and prevent bleeding with vitamin C The addition of closely related minerals (sodium, potassium, iron ..) help to absorb the necessary components in the body and at the same time maximize the role of osmotic action when the osteoporosis symptoms caused by the lack of minerals necessary for the body It is based on the synergistic effect of prophylactic prevention and treatment, as well as the significant reduction of body fat and blood cholesterol, that is, the skin extract containing hesperidin and the mineral extract extracted from seaweed.

First, in the present invention to prepare a peel of citrus fruits grown by the organic method to extract the peel extract from citrus peel. Conventional ethanol extraction method (Patent Publication No. 1998-027074) has a problem that can not be excluded that beneficial components such as ERIODICTIN, rutin (RUTIN) and the like contained in the citrus peel Since the present invention, by using only citrus peels grown by the organic method possible fermented fructose to exclude the pesticide component. In other words, when a shell is used to detect pesticides, even if a catalyst other than ethanol is used, a flavonoid-based component such as hesperdine cannot be detected as a heavy metal of the pesticide component.

Of course, the present invention not only utilizes citrus peels grown by the organic method of eliminating heavy metals in principle, but also by using conventionally grown citrus peels, even though residual pesticide components remain, the correlation between various reaction pathways, ATP Conjugation and energy production with the system, induction and regulation of enzymatic activity, further purification of individual enzymes and enzymatic chemical analysis of its mechanism of action, and co-factors such as thiamine pyrophosphate and NAD (coenzyme I) In addition to the ATP, cultivation is generally carried out by providing an enzyme extraction method in which an enzyme fermentation known to be involved in ions such as magnesium, calcium, potassium, or inorganic phosphate is removed by chemical reactions. You can also use citrus peels.

First, in order to extract an extract containing an active ingredient such as hesperdine from citrus peel, if possible, prepare citrus peel grown by organic method, dry it in the shade, and then dry the dried citrus peel. Drying citrus peels here is to prevent the destruction of vitamin P. The sugar is mixed with dried citrus fruit peel in an appropriate amount that can suppress the citrus inherent flavor and microbial activity during ripening, and fermented for a certain time. Hesperdine-containing skin extract can be extracted and used. Wherein the fermentation is made at room temperature, especially 15-25 ℃ 1-4 months, it is more preferably made in the shade.

In some cases, the citrus peel prepared as raw materials may be used as it is to extract the peel extract containing the active ingredient including hesperdine from the citrus peel.

The extracted skin extracts are hesperidin, eriodictin, rutin, pectin, limonene, isopropenyl toluene, 8-elemenene ( 8-elemenen), d- copaene (d- copaene), contains active ingredients such as erythrodrin.

In addition, in the present invention, in order to extract the mineral extract containing various mineral components including Na, Ca, K, Mg, Zn, Fe, Cu, Ni, Mn from the seaweed, the seaweed is prepared as raw or dry materials, The seaweed charcoal is made according to the charcoal manufacturing process, and the prepared seaweed charcoal is once again heat treated. Finely pulverized the seaweed charcoal produced by the reheating treatment, the powdered chopped seaweed charcoal powder and water are combined in a weight ratio of 1: 5-1: 10 and stirred to make charcoal. The charcoal is allowed to settle for a period of time to separate only the clear water from the top, and the separated water is evaporated to obtain a mineral extract.

Here, in extracting the mineral extract from the seaweed, instead of obtaining the extract directly from the seaweed, extracting the minerals contained in the seaweed by making charcoal may contaminate many heavy metals in seawater, and the seaweed may contain a large amount of harmful heavy metals. Therefore, while making charcoal and reheating, not only the heavy metals that are harmful to the human body are melted away and only the minerals beneficial to the human body remain, but also to obtain high purity and excellent absorption of minerals. That is, because the method used to dry the seaweed used in the prior art by adding the powder is not a method of extracting and using pure minerals because there was a disadvantage that the ionized mineral component can not be obtained.

In the following, examples are described to better describe the unique features of the present invention and how to perform them. However, the present invention should not be considered to be limited by the detailed description of the embodiments.

Example 1

Brown sugar is added to a tangerine peel (chopped unripe skin) in a weight ratio of 1: 2, stirred for about 1 hour, and left in the shade for 2 months at 20 ° C. for fermentation. The fermented material was filtered and discarded, and the filtrate was cooled at room temperature for about 30 minutes, and then the filtrate was stirred for 20 minutes, followed by a hot bath in water at 60 degrees Celsius to obtain a extract.

Example 2

Skin extract was obtained in the same manner as in Example 1 except that the tangerine peel was dried and ground.

Example 3

The seaweed was dried to make charcoal according to a conventional charcoal manufacturing process, and the charcoal produced by reheating at 1500 ° C was finely crushed to about 300 mesh. The crushed charcoal and water were mixed at a weight ratio of 1:10 and stirred for about 5 minutes to make charcoal. The prepared charcoal was left in a well-ventilated shade for 3 days, and the sunken impurities were discarded, and only the upper water was separated to evaporate the separation solution to obtain a mineral extract.

Example 4

From Example 2 to obtain a liquid skin extract with a moisture content of 70%, from Example 3 to obtain a mineral extract in the absence of any moisture to mix the liquid skin extract and the mineral extract in a weight ratio of 10: 1 A blend composition was prepared.

The skin extracts prepared in Examples 1 and 2 are effective in preventing cholesterol decomposition and osteoporosis harmful to the body by containing flavonoid-based components including hesperdine, and the mineral extracts prepared in Example 3 are natural As a mineral, it is obtained in a state of easy absorption in the body, and the composition of Example 4 is a composition in which the skin extract and the mineral extract can be synergistically compounded to further increase the pharmacological effect of hesperdine to further increase the harmful cholesterol. Since it has a remarkable effect such as degradation and prevention of osteoporosis, the extract or combination composition of Examples 1 to 4 can be used as a food additive effective in a variety of foods for health drinks or health promotion.

The present invention achieves the effect of providing an effective extraction method of the skin extracts including hesperidin and the skin extracts by providing an enzyme extraction method for more effectively extracting other active ingredients including hesperdine from the citrus peel.

The present invention also achieves the effect of effectively extracting various mineral components in an ionic state which are easily absorbed into the body from the seaweed and providing the mineral extract.

The present invention also achieves the effect of providing a blended composition in which the pharmacological effect of the extracts is increased by blending the extract and the mineral extract including hesperidin extracted in a predetermined ratio.

The present invention also achieves the effect of lowering harmful cholesterol levels in the body and providing the extract or combination composition which can be used as a food additive having an effect on the prevention and treatment of osteoporosis.

Claims (25)

Preparing a citrus peel; Combining the prepared citrus peel and sugar; Fermenting the blended citrus peel and sugar; Filtering the fermentation broth; And A method for extracting the skin extract, comprising the step of obtaining the extract from the filtrate. The method of claim 1, wherein the preparing of the citrus peel comprises the step of drying the citrus peel and pulverizing the shaded citrus peel. The method of claim 1, wherein the step of combining the prepared citrus peel and sugar is a method of extracting the extract of the skin extract, characterized in that the citrus peel and sugar is blended in a weight ratio of 1: 2-1: 10. The method of claim 1, wherein the sugar used in the step of blending the prepared citrus peel and sugar is fruit extract extract method characterized in that the fructose. The method of claim 1, wherein the step of fermenting the extract of the skin extract comprising fermenting at 1-25 months at 15-25 ℃. The method of claim 1, wherein the step of obtaining the rind extract comprises a step of evaporating the filtrate by boiling the filtrate in water of 55-65 ℃. Peel extract prepared by the method of any one of claims 1 to 6. 8. The extract of claim 7, wherein the extract contains hesperdine, rutin, pectin, and the like, which are effective ingredients for cholesterol degradation and osteoporosis prevention. Preparing the seaweed, Making the seaweed charcoal from the prepared seaweed, Reheating the seaweed charcoal, Pulverizing reheat treated seaweed charcoal, Mixing charcoal powder with water, and preparing the charcoal; Sinking the charcoal for a period of time to separate only the clear water above; And Mineral extract extraction method comprising the step of obtaining the mineral extract by evaporating the separated water. 10. The method of claim 9, wherein the forming of the charcoal is a method for extracting mineral extracts, characterized in that the blend of the powdered seaweed charcoal powder and water in a weight ratio of 1: 5-1: 10. Claim 9 mineral extract extracted by the extraction method. Mineral extract extracted by the extraction method of claim 10. The mineral extract according to claim 11 or 12, wherein the mineral extract comprises Na, Ca, K, Mg, Zn, Fe, Cu, Ni, Mn, or the like. A blend composition comprising the fruit extract of claim 7 and the mineral extract of claim 11 in a weight ratio of 10: 1 to 10: 5. A blend composition comprising the fruit extract of claim 7 and the mineral extract of claim 12 in a weight ratio of 10: 1 to 10: 5. A blend composition comprising the extract of claim 9 and the extract of claim 11 in a weight ratio of 10: 1 to 10: 5. A blend composition comprising the fruit extract of claim 9 and the mineral extract of claim 12 in a weight ratio of 10: 1 to 10: 5. 18. The blend composition according to any one of claims 14 to 17, wherein the blend composition comprises hesperdine, rutin, pectin and various mineral components. A method of using the fruit extract of claim 7 as a food additive. A method of using the fruit extract of claim 8 as a food additive. A method of using the mineral extract of claim 11 as a food additive. A method of using the mineral extract of claim 12 as a food additive. A method of using the mineral extract of claim 13 as a food additive. A method of using the compounding composition of any one of claims 14 to 17 as a food additive. A method of using the compound composition of claim 18 as a food additive.