KR20030078489A - Direct Urea Breath Test System For Detection of Helicobacter Pylori. - Google Patents

Direct Urea Breath Test System For Detection of Helicobacter Pylori. Download PDF

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KR20030078489A
KR20030078489A KR1020020017554A KR20020017554A KR20030078489A KR 20030078489 A KR20030078489 A KR 20030078489A KR 1020020017554 A KR1020020017554 A KR 1020020017554A KR 20020017554 A KR20020017554 A KR 20020017554A KR 20030078489 A KR20030078489 A KR 20030078489A
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박성한
김진철
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(주)위드교역
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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    • G01N33/497—Physical analysis of biological material of gaseous biological material, e.g. breath
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/08—Detecting, measuring or recording devices for evaluating the respiratory organs
    • A61B5/082—Evaluation by breath analysis, e.g. determination of the chemical composition of exhaled breath
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/08—Detecting, measuring or recording devices for evaluating the respiratory organs
    • A61B5/097—Devices for facilitating collection of breath or for directing breath into or through measuring devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/60—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances involving radioactive labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/62—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving urea

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Abstract

PURPOSE: A direct urea breath test system for detection of Helicobacter pylori is provided, thereby easily and cheaply diagnosing the infection of Helicobacter pylori. CONSTITUTION: A direct urea breath test method for detection of Helicobacter pylori comprises the steps of: orally administering radioisotope-labeled urine into a patient; and directly blowing to a direct counting gas flow typed detector to detect radiation gas in the breath, wherein the radioisotope is 14-C and the radiation gas is 14CO2; and the direct counting gas flow typed detector is a GM(Geiger-Muller) counter or a proportional counter.

Description

헬리코박터 파이로리의 요소호흡 직접검사법{Direct Urea Breath Test System For Detection of Helicobacter Pylori.}Direct Urea Breath Test System For Detection of Helicobacter Pylori.

본 발명은 헬리코박터 파이로리(Helicobacter pylori, 이하 H. pylori 라 함)를 검사하기 위한 기술로서, 방사성동위원소인 C-14를 표지한 요소를 섭취한 후 반응을 알기위해 호기(입)에 검출기를 직접 밀착하여 호기 검사하는 방법에 관한 것이다.The present invention is a technique for examining Helicobacter pylori (hereinafter referred to as H. pylori), the detector is placed directly in the exhalation (mouth) to know the reaction after ingesting a radioactive isotope C-14 labeled element The present invention relates to a method of inspecting exhalation closely.

H. pylori는 1983년 Dr. Warren과 Dr. Marshall에 의해 처음으로 발견되었으며, 나선형 몸통과 4～8개의 편모를 가지고 있는 그람음성세균으로서 위에 감염되어 다양한 위장관 질환(위염, 위궤양, 위암 등)과 십이지장, 췌장에도 영향을 끼치는 원인균으로 알려져 있다.H. pylori was published in 1983 by Dr. Warren and Dr. It was first discovered by Marshall and is a Gram-negative bacterium with a spiral trunk and 4 to 8 flagellae. It is known as a causative agent that affects various gastrointestinal diseases (gastritis, gastric ulcers, gastric cancer, etc.), duodenum and pancreas.

H. pylori의 진단방법은 체내에 기구를 삽입하는 침습법과 삽입하지 않는 비침습법이 있다. 침습법에는 내시경검사 후 위조직을 생검하여 균 유무를 조사하는 조직검사방법, 생검 후 균을 배양하는 균배양방법, 체내조직 내부에서 우레아제의 활성도를 측정하는 RUT (Rapid Urease Test)방법 등이 있으며, 비침습법은 환자의 혈액 등을 채취하여 검사하는 혈청학적 검사방법과 환자에게 요소를 투여한 후 호흡기로 배출되는 가스를 측정하는 요소호기검사(Urea Breath Test, 이하 UBT)방법이 있다.The diagnostic methods of H. pylori include invasive methods of inserting instruments into the body and noninvasive methods of not inserting. Invasive methods include biopsy of gastric tissue after endoscopy to examine the presence of bacteria, culture of bacteria to culture bacteria after biopsy, and rapid urease test (RUT) to measure the activity of urease inside the tissues. For example, non-invasive methods include a serological test method for collecting and testing a patient's blood and a urea breath test (UBT) method for measuring a gas discharged to the respirator after urea is administered to the patient.

침습법은 내시경검사 및 생검으로 인한 환자의 고통으로 인하여 현재는 그 사용이 감소하고 있는 추세이며, 비침습법의 혈청학적 검사방법 또한 민감도와 특이도 등의 검사 정확도가 떨어지며 조기판정이 불가능한 점 등으로 보편화되지 못하고 있다. 따라서 UBT법은 환자에게 불쾌감을 유발하지 않으면서 민감도와 특이도가 우수하며 신뢰성이 있는 점 등으로 최근 사용자가 급증하고 있으며 또한 H. pylori 감염의 진단뿐만 아니라 제균요법 후 치료의 효과를 판정하는데 가장 뛰어난 방법으로 선진국에서 보편화된 검사법이다.Invasive methods are currently being used due to the pain of patients due to endoscopy and biopsy, and the non-invasive serological test method also lacks sensitivity and specificity, and early detection is impossible. It is not universal. Therefore, the UBT method has been rapidly increasing in users due to its excellent sensitivity, specificity, and reliability without causing discomfort to patients, and is most effective in determining the effectiveness of treatment after disinfection therapy as well as diagnosis of H. pylori infection. It is an excellent method that is common in developed countries.

UBT법은 요소표지 동위원소의 종류에 따라 C-13 UBT와 C-14 UBT로 나누어지며 사용비율은 6:4정도로 C-13 UBT가 근소한 우세를 보이고 있으며, 그 이유는 C-14 UBT의 경우 저렴한 검사장비에도 불구하고 표지 동위원소인 C-14가 방사성동위원소로 안전관리 측면에서 다소간의 규제를 받고 있기 때문이다. 그러나 C-13 UBT는 검사장비가 고가이므로 선진국을 제외하고는 보편화되지 못하고 있다.The UBT method is divided into C-13 UBT and C-14 UBT according to the type of element-labeled isotopes. The ratio of use is 6: 4, and C-13 UBT shows a slight advantage, because of C-14 UBT. Despite the inexpensive inspection equipment, the labeling isotope, C-14, is a radioisotope that is somewhat regulated in terms of safety management. However, C-13 UBT is not common except in developed countries because of the high cost of inspection equipment.

상기 기재된 방법의 문제점들에 대한 개선방법으로 C-13 UBT의 경우는 중저가의 검사장비를 개발하려는 연구가 활발히 진행되고 있지만 그마저 C-14 UBT 검사장비와의 가격격차는 여전히 큰 차이를 보이고 있어 검사비용 측면에서 C-14 UBT의 활용방안이 최근 대두되고 있는 실정으로 C-14 UBT의 문제점인 경구 투여시 요소에 표지된 방사성동위원소의 량을 규제 면제량 이하로 감소시키기 위해서 저 방사선량 측정이 가능한 측정방법의 개선이 필요하게 되었다.In the case of the C-13 UBT, researches are being actively conducted to develop low and low cost inspection equipment as an improvement method for the problems described above, but the price gap with the C-14 UBT inspection equipment still shows a big difference. In view of the cost of examination, the use of C-14 UBT has recently emerged. In order to reduce the amount of radioisotope labeled in urea to below the regulatory exemption level during oral administration, a problem of C-14 UBT, There is a need for improvement of this possible measurement method.

본 발명의 목적은 C-14 UBT법의 문제점인 요소에 표지된 C-14의 량을 규제 면제량 이하(면제량의 1/2 수준)로 유지해야 할 필요가 있으며, 따라서 기존보다 적은량이 경구 투여됨에 따라 호흡기를 통해 배출하는 가스중의 C-14 방사능 값이 상대적으로 작을 것이므로 이를 효과적으로 포집하고 포집된 가스를 고효율로 측정하는 검사장비의 개발이 필요하게 되었으므로 본 발명 장비인 호기에 검출기를 직접 밀착하여 검사하는 C-14 UBT 장비를 개발하려 한다. 이 방법은 직접측정 방법이므로 기존방법이 간접측정 방법으로 전처리 및 측정절차가 복잡하여 숙련된 검사자가 필요하였지만 이러한 단점을 모두 해결할 수 있다는 측면에서 기술적 가치가 크다.An object of the present invention is to maintain the amount of C-14 labeled on the urea, which is a problem of the C-14 UBT law, to be below the exemption level (half the level of the exemption), so that the amount less than the conventional Since the C-14 radiation value in the gas discharged through the respiratory system will be relatively small as it is administered, it is necessary to develop a test equipment that effectively captures the collected gas and measures the collected gas with high efficiency. We are going to develop a C-14 UBT device that will inspect closely. Since this method is a direct measurement method, the existing method is an indirect measurement method, which requires a skilled inspector due to the complicated pretreatment and measurement procedures, but it has great technical value in that it can solve all these disadvantages.

제1도는 본 발명의 헬리코박터 파이로리(Helicobacter pylori) 요소호흡 직접 검사를 위한 검출장치를 나타내는 도이다.1 is a diagram showing a detection device for the direct examination of Helicobacter pylori urea breath of the present invention.

제2도는 기존에 사용되고 있는 C-14 UBT 검사법의 순서도이다.2 is a flow chart of the C-14 UBT test method used in the past.

제3도는 본 발명의 직접 검사법으로 인해 제2도의 복잡한 검출단계가 생략된 도이다.3 is a diagram in which the complicated detection step of FIG. 2 is omitted due to the direct inspection method of the present invention.

제4도는 C-14 UBT검사의 결과로서, 기존의 PY test법에 의한 LSC 측정값과 본 발명의 직접검출법에 의한 Scaler 측정값을 서로 비교한 도이다.4 is a result of the C-14 UBT test, a comparison of the LSC measurement value by the conventional PY test method and the scaler measurement value by the direct detection method of the present invention.

본 발명의 검사절차는 우선 방사성동위원소인 C-14가 표지된 요소를 경구투여한 후 호기 배출까지의 과정은 기존의 C-14 UBT법과 동일하다. 즉 C-14가 표지된 요소를 경구 투여하면 위내의 H. pylori가 분비하는 우레아제와 가수분해하여14CO2가 발생되어 이를 호기를 통해 배출하며, 반면에 H. pylori 비 보균자의 경우는 우레아제가 분비되지 않으므로 경구 투여된 요소는 거의 대부분 소화기로 흡수된 후 배설되어 호기에14CO2가 발생되지 않는다.In the inspection procedure of the present invention, the procedure of orally administering a radioactive isotope C-14 labeled element first to aerobic discharge is the same as that of the conventional C-14 UBT method. In other words, oral administration of C-14-labeled urea hydrolyzes with the urease secreted by H. pylori in the stomach to produce 14 CO 2, which is excreted through exhalation, whereas in non-H. Pylori carriers, urease Since it is not secreted, almost all orally administered urea is absorbed by the digestive system and then excreted to produce 14 CO 2 during expiration.

따라서 호기로 배출되는14CO2중의 방사성동위원소인 C-14를 방사선카운터로 측정하여 방사선량의 기준치 초과 유무로 H. pylori 보균 여부를 판단하는 것으로 호기 배출 이후 절차 및 측정방법이 기존의 방법과 큰 차이를 보인다.Therefore, C-14, a radioisotope in 14 CO 2 discharged to aerosols, is measured with a radiation counter to determine whether H. pylori is present with or without the radiation dose threshold. It makes a big difference.

본 발명의 호기 배출과정 이후의 절차는 기존 C-14 UBT법에서 채택하고 있는 풍선 또는 필터를 이용한14CO2가스의 포집 및 액화 등의 전처리 과정이 생략되며 곧바로 호기 배출14CO2가스를 검출기에 불어넣어 주고, 검출기 내부로 들어온14CO2가스중 방사성동위원소인 C-14에서 방출되는 방사선이 검출기 내부에 별도로 주입되는 계수용 기체와 상호작용을 일으켜 펄스가 얻어지며, 고전압의 인가전압을 조절하여 펄스 파고를 선별한 후 계수장치에서 유효 펄스를 계수함으로서 측정이 종료된다.Aerobic process after the discharge process of the present invention to an existing C-14 capture and are a pre-processing such as liquefaction is omitted detector a straight exhalation discharge 14 CO 2 gas in the 14 CO 2 gas using a balloon or a filter that is employed in the UBT method Blowing in, the radiation emitted from C-14, a radioisotope of 14 CO 2 gas entering the detector, interacts with the counting gas injected into the detector separately, and a pulse is obtained. After the pulse crest is sorted, the measurement is completed by counting the effective pulses in the counter.

본 발명은 「도면1」에서 보는 바와 같이 C-14 UBT에서 호기 배출되는14CO2를 검출하는 검출장치(검출기)로서, 주요 구성은 연속 플로우형GM(Geiger-Mueller) 또는 비례계수기형의 검출기를 응용한 것으로 호기로 배출되는14CO2가스의 직접 검출을 위해 호기(입)에 검출기를 직접 물고14CO2가스를 불어넣는 부분(1),14CO2가스 주입 때에만 개방되고 그 외의 때에는 계수용 가스를 주입하는 압력에 의해 막혀 있도록 만든 부분(2), 계수용 가스 및14CO2가스의 배기를 위한 스위치로서 스위치를 누르면 배기구가 열려 가스가 배출되며 아울러 계수기의 타이머와 연동되어 계수가 시작되도록 만든 부분(3)으로 구성되어 있으며, 이때 스위치를 놓으면 계수가 중단되도록 되어 있다.The present invention is a detector (detector) for detecting 14 CO 2 discharged from aerobic C-14 UBT as shown in Figure 1, the main configuration is a detector of a continuous flow type GM (Geiger-Mueller) or proportional counter type a by biting a detector in the exhalation (I) for the direct detection of the 14 CO 2 gas discharged from the exhalation directly and only open part (1), when the 14 CO 2 gas inlet of blowing the 14 CO 2 gas at that time other than the application A switch for exhausting the part 2, the counting gas and the 14 CO 2 gas, which is blocked by the pressure to inject the counting gas, and when the switch is pressed, the exhaust port is opened to exhaust the gas, and the counting is started in conjunction with the counter of the counter. It is composed of the part (3) made, and the count is stopped when the switch is released.

그 외 주요구성은 기존의 가스 플로우형 비례계수기에 적용되는 방법을 응용한 것으로 계수용 가스의 저장을 위한 부분(4)과 계수용 가스를 검출기 내부로 주입하기 위한 압력조절 부분(5)이 있으며 사용되는 계수용 가스는 불활성 기체인 아르곤을 주성분으로 검출기 내부의 전자눈사태 등을 방지하기 위한 소멸(Quenching)목적으로 염소를 소량 첨가시켜 만든 혼합가스(아르곤 95%, 염소 5%)를 사용한다.The other main components are applied to the existing gas flow type proportional counter, and there is a part 4 for storing the counting gas and a pressure adjusting part 5 for injecting the counting gas into the detector. The counting gas uses a mixed gas (argon 95%, chlorine 5%) made by adding a small amount of chlorine for the purpose of quenching to prevent electron avalanches in the detector, mainly based on argon, which is an inert gas.

또한 검출기 내부에서는14CO2가스와 계수용 가스가 상호작용하여 이온화된 양이온과 전자의 이온쌍을 형성시키는 전리작용을 일으키며, 이때 전자가 양극으로 이동하여 출력펄스를 형성시키는 양극도선 부분(6), 형성된 출력펄스를 고전압 전원 및 계수장치와 연결시키는 부분(7)으로 구성되어 있다.In addition, inside the detector, 14 CO 2 gas and the counting gas interact with each other to cause ionization to form ionized cations and electron ion pairs, where the electrons move to the anode to form an output pulse (6), It consists of a part 7 for connecting the formed output pulses with a high voltage power supply and a counting device.

본 발명의 C-14 UBT 장치의 특징은 「도면 2」와 「도면3」에서 보는바와 같이 기존의 간접 측정법을 직접 측정법으로 변경 설계한 것으로 기존의 간접측정은 제한된 량의 시료를 사용하는 것과 번거로운 전처리 절차를 거치는 등의 문제점이 있었으나 본 발명의 직접측정은 호기로 배출되는14CO2가스를 검출기 내부로 직접 불어넣어 연속적으로 많은 량을 직접측정 할 수 있으며, 검출장치의 형식이 4π가스 플로우형 GM 또는 비례계수기로서 검출기 내부로 들어오는14CO2가스는 거의 대부분 계수되어 높은 계수효율을 가진다.The characteristics of the C-14 UBT device of the present invention are designed by changing the existing indirect measurement method to a direct measurement method as shown in Figs. 2 and 3, and the conventional indirect measurement is cumbersome to use a limited amount of samples. Although there was a problem such as going through a pretreatment procedure, the direct measurement of the present invention can directly measure a large amount continuously by directly blowing 14 CO 2 gas discharged into the detector into the detector, and the type of detection device is a 4π gas flow type. The 14 CO 2 gas entering the detector as a GM or proportional counter is almost always counted and has a high counting efficiency.

따라서 높은 계수효율로 인해 비방사능이 낮은 물질에서도 효과적이므로 검사를 위해 경구투여 하는 요소의 량을 적게 할 수 있으며 아울러 요소에 표지하는 방사성동위원소인 C-14의 방사능량도 규제 면제치의 1/2 수준 이하로 낮추어 표지 할 수 있다. 즉 기존의 검출장치 형식인 액체섬광계수기(LSC : Liquid Scintillation Counter)와 계수효율 등의 성능을 비교하였을 때 조금도 뒤떨어지지 않으며, 반면 방사선안전 문제의 해소와 검사절차의 간소화를 이룰 수 있으며 검출장치의 가격은 기존 액체섬광계수기와 비교하여 1/8～1/10 수준으로 아주 저렴하다는 점이 큰 장점이다.Therefore, due to the high counting efficiency, it is effective in substances with low specific radioactivity, so that the amount of orally administered urea for testing can be reduced, and the radioactive amount of C-14, the radioisotope labeled on the element, is also 1/2 of the exemption level. Can be lowered below the label level. That is, when comparing the performance of the liquid scintillation counter (LSC), which is a conventional detection device type, with the counting efficiency, it does not lag behind.However, it is possible to solve the radiation safety problem and simplify the inspection procedure. Compared with the existing liquid scintillation counter, the price is very low (1/8 to 1/10).

그러나 검출기가 가스 플로우형으로 측정을 위해서는14CO2가스를 별도 계수용 가스와 함께 연속적으로 검출기 내부로 흘려주어야 하므로 기존 방법에 비해 긴시간(대략 7초 정도로 5회 반복) 동안 호기로14CO2가스를 배출하여 검출기 내부에 불어 주어야 하는 불편함이 있다.However, because the detector need to shed the 14 CO 2 gas in order to measure a gas flow type as a detector inside a row with a separate gyesuyong gas in the exhalation for long periods of time (repeated five times to approximately 7 seconds) compared to the conventional method 14 CO 2 gas There is an inconvenience to be discharged to blow the inside of the detector.

〈실시예〉<Example>

1. 측정장치 제작 및 준비1. Manufacturing and preparation of measuring devices

H. pylori의 요소호흡 직접검사를 위한 검출장치의 제작은 W.B. Johnson & Associates Inc.의 Detector(Model : GP200)를 「도면1」과 같이 개조 하였고 계수장치는 W.B. Johnson & Associates Inc.의 Scaler(Model : DIG-6)를 그대로 사용하였다.Fabrication of a detection device for the direct urea breath test of H. pylori is described in W.B. Johnson & Associates Inc.'s Detector (Model: GP200) was modified as shown in Figure 1, and the counter was W.B. Johnson & Associates Inc. Scaler (Model: DIG-6) was used as it is.

또한 비교측정을 위한 검출기구는 Ballard사의 PY test kit을 사용하였고, 계수장치는 Beckman사의 LSC(LS 6500)를 사용하였다.In addition, Ballard's PY test kit was used as a detector for comparative measurement, and Beckman's LSC (LS 6500) was used as a counter.

H. pylori의 요소 호흡 검사를 위해 경구 투여할 C-14가 표지된 요소는 Ballard사의 PY test용14C-Urea Capsules을 사용하였다.The C-14-labeled urea to be administered orally for the urea respiration test of H. pylori was used by Ballard's 14 C-Urea Capsules for PY test.

2. 비교측정 방법2. Comparative measuring method

검사 대상자를 8명 선정하여 각각14C-Urea Capsules을 20mL 정도의 물과 함께 경구투여 하고 3분후 다시 20mL 정도의 물을 제차 복용한 후 위 속에서의 반응을 위해 5분정도 기다린 후 먼저 PY test Kit의 Balloon에 2L정도14CO2가스를 불어넣고 봉입한 후 곧바로 직접 검사를 위해 제작한 「도면1」 의 검출장치에 7초동안14CO2가스를 불어넣는 동작을 5회 반복하여 Scaler의 계수를 총 35초 동안 하여 측정값을 얻은 후 분당 계수율(cpm)로 환산하였으며, 봉입한 Balloon내의14CO2가스는 PY test Kit을 사용한 포집 및 액화 등의 과정을 거쳐 LSC로 1분간 계수하여 cpm단위로 측정값을 얻었다.Eight people were selected and 14 C-Urea Capsules were orally administered with 20 mL of water, and after 3 minutes, another 20 mL of water was taken again. After waiting for 5 minutes for reaction in the stomach, PY test Inject 2L of 14 CO 2 gas into the balloon of the kit and immediately insert it into the detector of `` Figure 1 '', which is designed for direct inspection, and blows 14 CO 2 gas for 7 seconds for 5 seconds. After obtaining the measured value for a total of 35 seconds and converted to count per minute (cpm), 14 CO 2 gas in the balloon is counted for 1 minute by LSC through the process of capturing and liquefaction using PY test kit, cpm unit The measured value was obtained.

3. 결과3. Results

「도면4」에서 보는바와 같이 기존방법(LSC)과 직접검사 방법(Scaler)의 관계를 비교 검토한 결과 약간의 차이는 있으나 거의 비례적인 Data값을 얻었다.As shown in Figure 4, the relationship between the existing method (LSC) and the direct inspection method (Scaler) was examined. As a result, the data were almost proportional.

이 결과를 바탕으로 검사 대상자의 H. pylori균의 감염 여부를 판단하는데 직접 검사법을 사용 하여도 충분한 검사 정확도를 얻을 수 있음을 확인하였다. 즉 H. pylori균 감염자는 높은 수치의 계수율을 보인 반면 비감염자는 거의 Background에 가까운 계수율을 보였다.Based on these results, it was confirmed that sufficient test accuracy could be obtained even if the direct test method was used to determine the infection of H. pylori. In other words, those infected with H. pylori showed a high count rate while those who were not infected had almost the count rate.

본 발명에 따라 요소호흡 직접검사가 가능하게 되어 H. pylori균의 감염 여부를 손쉽게 진단할 수 있게 되었으며, 요소에 표지하는 방사성동위원소의 량을 줄일 수 있어 방사선 안전 문제를 조금이나마 해소하였다.According to the present invention, the urea breathing test was made possible to easily diagnose the infection of H. pylori bacteria, and the amount of radioisotope labeled on the urea could be reduced to solve the radiation safety problem.

또한 검사장비의 가격이 기존장비에 비해 아주 저렴함으로 기존 C-13 UBT, C-14 UBT 방법이 편리성과 뛰어난 신뢰성에도 불구하고 검사장비의 고가로 그 사용이 제한적(대형 종합병원 위주) 이었던 것을 소규모의 내과병원에서도 경제적 부담없이 사용할 수 있게 되었다는 것이다.In addition, the price of inspection equipment is much lower than that of existing equipment, so the existing C-13 UBT and C-14 UBT methods are limited due to the high cost of inspection equipment despite their convenience and excellent reliability. It can be used without a burden on the medical hospital of the hospital.

Claims (4)

C-14가 표지된 요소를 경구투여 한 후 발생되는14CO2가스를 호흡기로 직접 계수가스 플로우형 검출기에 불어넣어 감염여부를 측정하는 방법.Method of measuring the infection by blowing 14 CO 2 gas generated after oral administration of a C-14-labeled element directly to the counting gas flow detector with a respirator. 방사성동위원소 표지물질의 경구투여 후 발생하는 방사성가스를 호흡기로 직접 계수가스 플로우형 검출기에 불어넣어 방사선량을 정량 측정하는 방법.Method of quantitatively measuring radiation dose by blowing radioactive gas generated after oral administration of radioisotope labeling substance directly into the counting gas flow detector with a respirator. 제2항의 계수가스 플로우형 검출기는 GM(Geiger-Mueller) 및 비례계수 방식이며, 그 외 직접 계수를 위한 검출기 방식에 반도체 계수방식과 섬광 계수방식을 포함한다.The counting gas flow detector of claim 2 is a GM (Geiger-Mueller) and a proportional counting method, and the detector method for direct counting includes a semiconductor counting method and a scintillation counting method. 「도면1」의 형태로 된 가스 플로우형 검출장치Gas flow detection device in the form of "Fig. 1"
KR1020020017554A 2002-03-29 2002-03-29 Direct Urea Breath Test System For Detection of Helicobacter Pylori. KR20030078489A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100692959B1 (en) * 2005-02-28 2007-03-14 주식회사 엔바이로코리아 Method for Urea Breath Test Using Liquid Scintillation Counter
CN101231284B (en) * 2007-01-23 2012-09-26 深圳市先亚生物科技有限公司 Urea kit for diagnosing pylorus helicobacterium infestation
CN108333336A (en) * 2018-01-12 2018-07-27 大连交通大学 A kind of novel helicobacter pylori detector
CN111599457A (en) * 2020-06-18 2020-08-28 深圳市人民医院 Self-service noninvasive helicobacter pylori detection system and method

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100692959B1 (en) * 2005-02-28 2007-03-14 주식회사 엔바이로코리아 Method for Urea Breath Test Using Liquid Scintillation Counter
CN101231284B (en) * 2007-01-23 2012-09-26 深圳市先亚生物科技有限公司 Urea kit for diagnosing pylorus helicobacterium infestation
CN108333336A (en) * 2018-01-12 2018-07-27 大连交通大学 A kind of novel helicobacter pylori detector
CN111599457A (en) * 2020-06-18 2020-08-28 深圳市人民医院 Self-service noninvasive helicobacter pylori detection system and method

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