KR20030013530A - Method for generating music by dna a dna base array and providing it's service - Google Patents

Abstract

PURPOSE: A creation of a music by a DNA base arrangement and a method for supplying a service using the same are provided to create a music by mapping genetic information included in a DNA having a characteristic between individuals with music information in accordance with a DNA base arrangement analysis result and to supply a DNA music service of an individual through the Internet using an on-line/off-line technique. CONSTITUTION: In a system related to a base sequence extraction through a DNA analyzer, a DNA base sequence is extracted through a commonly used DNA analyzing method using a conventional DNA analyzer(S1). The DNA base sequence is converted into a music using a conventional music creating tool, and an inherent DNA music file is created according to individuals(S2). The created DNA music file is stored in a database(S3). The music file is distributed to an off-line user(S4), or the music file is transmitted to an on-line user computer on an Internet network through a web server(S5).

Description

Music generation using DNA base sequence and service providing method using same {METHOD FOR GENERATING MUSIC BY DNA A DNA BASE ARRAY AND PROVIDING IT'S SERVICE}

The present invention relates to music generation by DNA nucleotide sequence and a service providing method using the same, in particular, according to the DNA nucleotide sequence analysis results, by mapping the genetic information contained in the DNA having uniqueness between individuals to music information to generate music The present invention relates to a music generation method using a DNA nucleotide sequence that provides an individual DNA music service through the Internet using online / offline technology, and a service providing method using the same.

In general, similar techniques to solve the problem of converting genetic information into music information by mapping the genetic code contained in DNA to music features and to highlight uniqueness among individuals, which is one of the DNA characteristics, have not been attempted yet. There is no prior art.

<Synthesis of Functional Conductive Polymer and DNA Detection Method Using the Same>

① An example of the prior art.

1. Measure the intensity of fluorescent material using opticl method by hybridizing target DNA (or oligonucleotide) labeled with fluorescent material (dye) to fixed probe DNA.

(end). Characterization of DNA hybridization on the optical fiber surface, Colloids and surfaces A: Physicochemical and Engineering Aspects, 2000, 175, 147-152

(I). Fiber-optic genosensor for specific determination of femtomolar DNA oligomers, Analytica Acta, 1997, 350, 51-58

(All). Surface-Enhanced Raman Gene Probes, Anal. Chem. 1994, 66, 3379-3383

2. Hybridize the fixed probe DNA with the target DNA, and react with the indicator again so that the indicator is inserted into the double-stranded DNA.

(end). DNA electrochemical Biosensor for the detection of short DNA sequences related to the human immunodeficiency virus, Anal. Chem., 1996 , 68, 2629-2634

(I). Sequence-Specific Gene Detection with a Gold Electrode Modified with DNA Probes and an Electrochemically Active Dye, Anal. Chem. 1994, 66, 3830-3833

(All). DNA Sensing on a DNA Probe-Modified Electrode Using Ferrocenylnaphthalene Diimide as the Electrochemically Active Ligand, Anal. Chem., 2000, 72, 1334-1341

② another conventional example.

1. Fix the probe DNA on the electrode surface and measure the change in frequency when reacting with the target DNA using QCM.

(end). In-situ measurement of DNA immobilization and hybridization using a 27 MHz quartz crystal microbalance, Colloids and Surfaces B: Biointerfaces, 1998, 10, 199-204

(I). Hybridization of Nucleic Acids Immobilized on a Quartz Crystal Microbalance, J. Am. Chem. Soc., 1992, 114, 8299-8300

2. Fix the probe DNA on the polypyrrole probe and measure the potential shift and current magnitude change of the redox wave of the conductive polymer itself when reacting with the target DNA.

(end). Toward Bioelectronics: Specific DNA Recognition Based on an Oligonucleotide- Functionalized Polypyrrole, J. Am. Chem. Soc., 1997, 119, 7388-7389

(I). Toward intelligent polymers: DNA sensors based on oligonucleotide-functionalized polypyrroles, Synthetic Metals, 1999, 100, 89-94

<DNA detection method>

① DNA hybridization detection method using impedance

▶ DNA is done spirally turned in two of the strands. The hybridization of a single strand of ssDNA with another ssDNA of the same sequence complementary to each other is called hybridization. DNA hybridization detection refers to a technique for screening differences before and after hybridization when a hybridization reaction with a target DNA complementary to a single strand of probe DNA immobilized on a solid substrate surface.

FIG. 5a shows a probe (single-strand sequence immobilized on the electrode surface), and the impedance is measured in pH 7.0 phosphate buffer solution containing 0.75 M NaCl from 100 kHz to 10 Hz. The electrode was reacted with a target (single strand sequence to react with a probe) for 30 minutes and the impedance was measured under the same method and conditions. After reacting with the target, the impedance value decreased a lot ((A) of FIG. 5A). This can be easily seen that the difference between before and after hybridization is also represented by another form of admittance (FIG. 5A (B)). In Fig. 5A (A), the resistance value of the y-axis decreases after hybridization, which means that the conductivity is better.

Figure 5b is a graph showing the difference between the logarithmic impedance value before and after hybridization after the hybridization reaction sequence E having a completely different base sequence. In case (A) of FIG. 5B, the difference value is close to zero. This is because no hybridization has occurred, which means that there is no change in impedance after the hybridization reaction. However, when the electrode is hybridized with target sequence B, which is completely complementary, the difference is clearly shown (Figure 4-2b). This means that hybridization has taken place and there is a large difference in impedance values after hybridization. Therefore, it was possible to clearly distinguish between target sequence B and sequence E, which are completely complementary.

Figure 5c shows one base sequence mismatched in the middle (3'-GAGGAC T CCTCTTCAGACG-5 '; sequence C) and two sequences mismatched at the end (3'- CT GGACACCTCTTCAGACG-5'; sequence D) This is a graph showing the difference between the logarithmic impedance values before and after hybridization after hybridization. The results of repeated experiments three times for each sequence are shown. In both cases, the impedance value is slightly changed after the hybridization reaction. However, the magnitude of the difference was not so great compared to the difference in the complete complement sequence (Sequence B). Therefore, the hybridization detection method using impedance can distinguish complete and mismatched sequences.

However, similar conventional technologies include a technique for converting speech media into text media and text media into sound media. However, the present invention is a technique for converting a gene media into a music media and a music media into a gene media. Can't.

However, there is no technology for converting genetic information into sound information by mapping genetic codes included in DNA to music features, and there is a problem of converting genetic media into music media.

The present invention has been proposed to solve the problems of the prior art, and an object of the present invention is to solve the problem of converting genetic information into sound information by mapping the genetic code contained in the DNA to a musical feature and one of the DNA characteristics. By highlighting the uniqueness among individuals, you can give your own unique music and distinguish individuals with your own unique music, and convert DNA sequence into music media to analyze DNA characteristics as music and to express the natural sound of DNA itself. For example, by converting unique DNA characteristics to sound, each individual has their own unique music, which provides music creation by using DNA sequencing to distinguish individuals with unique music, and provides a service providing method using the same. will be.

1 is a block diagram of generating DNA music according to the present invention and providing the result to Internet users.

Figure 2 is a DNA music production tool according to an embodiment of the present invention.

3 is a music file resulting from mapping DNA genetic information to a music file using a DNA music generation tool.

4 is a flowchart illustrating a music generation method using a DNA base sequence and a service providing method using the same.

<Description of Symbols for Main Parts of Drawings>

1: DNA sequence 2: music generator

3: result music file 4: service DB

5: visitor 6: web server

7: your computer

In order to achieve the object of the present invention, the present invention relates to a system for extracting the base sequence through a DNA analyzer: extracting the DNA sequence through a commercial DNA analysis method using a conventional DNA analyzer (S1); Generating a DNA music file unique to each individual by converting the DNA sequence into music using a DNA music generating tool (S2); Storing the generated DNA music file in a database (S3); And distributing the music file to the visited user offline (S4), or transmitting the DNA music file to an online user's computer through the web server to the Internet network (S5). It provides a music generation and service providing method using the same.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

1 is a block diagram of generating DNA music according to the present invention and providing the result to Internet users.

By extracting the DNA sequence (1) unique to each individual of the existing DNA analyzer through a commercialized DNA analysis method by converting the DNA base sequence (1) to a music file through the DNA music generation tool (2) according to the present invention The resulting music file 3 is generated and stored in the service database 4.

The generated DNA result music files 3 are distributed to users who have visited the service database 4 offline, or the DNA music is distributed to the online user computer 7 via the web server 6 over the Internet.

In the present invention, a single-stranded probe is immobilized on the electrode surface by using a DNA sequence related to sickle cell disease, and the complementary sequences (sequence B, sequence C, sequence D, and sequence E) are respectively phosphate buffered. The two values were compared by hybridizing at and measuring the impedance before and after hybridization. The difference between the two values was calculated using the logarithmic impedance value taken as the log of the measured impedance before and after hybridization. The target sequence was determined between the completely complementary sequence (3'-GAGGACACCTCTTCA GACG-5 '; sequence B) and the sequence. Mismatch of one nucleotide sequence (3'-GAGGAC T CCTCTTCAGACG-5 '; sequence C), mismatched two sequences at the end (3'- CT GGACACCTCTTCAGACG-5'; sequence D), and nucleotide sequence These completely different ones (3'- CCTAGTCTACAGGTCACTA- 5 '; sequence E) were compared.

<Experimental Result of the Present Invention>

Selectivity is a matter of how to distinguish fully matched and mismatched sequences. In the present invention, the degree of hybridization was shown by comparing the magnitudes of the logarithmic impedance difference values before and after hybridization at 1 kH, and the degree of hybridization showed a selectivity for a specific sequecne. The results of the experiment of the present invention are shown in Table 1 below.

Hybridization amounts between the probe-immobilized electrode and Various 19-mer Oligonucleotide in solution at 1kHz Oligonucleotides Sequence ^b Z | / 10 ⁿ Ohm % hybridization ^c complementary (sequence B) 3-GAG-GAC-ACC-TCT-TCA-GAC-G-5 0.7 ± 0.05 100% One-base mismatch (sequence C) 3'-GAG-GAC-TCC-TCT-TCA-GAC-G-5 ' ^`` 0.1 ± 0.05 14.3% End two-base mismatch (sequence D) 3'-CTG-GAC-ACC-TCT-TCA-GAC-G-5 ' 0.1 ± 0.05 14.3% Noncomplementary (sequence E) 3'-CCT-AGT-CTA-CAG-GTC-ACT-A-5 ' 0.0 ± 0.05 0 %

a) The probe oligonucletide of NH2 -C6 5-CTC-CTG-TGG-AGA-AGT-CTG-C-3 was immoblized on the polyterthiophene modified glassy carbon electrode.

b) Concentration: 110 nmol in 10 ml.

c) Indicates how much of the oligonucleotide in the solution bound to the probe on the modified electrode

<Comparative DATA>

In 1992, Okahata's team used hybridization detection of various 10-mer nucleotides using QCM. In this experiment, the end 2-mer mismatched sequence showed 92% hybridization and the center 1-mer mismatched sequence showed about 30% hybridization (see Table 2). However, in the results of this experiment, hybridization of 14.3% was observed as shown in Table 1. Therefore, it can be seen that hybridization can be selectively discriminated with respect to a target sequence in the case of hybridization detection using impedance.

FIG. 2 shows a DNA music generating tool according to an embodiment of the present invention, and FIG. 3 shows a music file as a result of mapping DNA genetic information to a music file using the DNA music generating tool.

The present invention substitutes codons and characteristics of the nucleotide sequence, which is a component of DNA, to various characteristics of music, thereby converting natural information represented by DNA into music information. Furthermore, the first-converted music is combined with the individual genetic characteristics and the individual macroscopic characteristics of humans and substituted into the musical elements to create individual music. To this end, it creates a tool that can convert DNA sequences into music, and this tool supplies individual music to individuals so that they can have their own music.

Therefore, the present invention is to make a new attempt to convert the genetic information into music and to reversely convert the music into genetic information according to the result.

The DNA nucleotide sequence is represented by four letters, A, C, G, and T, and these four letters are combined into three pairs of one code. T may also be represented as U. Create a basic note by substituting the pitch with the pitch, length, and loudness. At this time, apply inversion or retrograde to prevent pitch and beat progressing equally. In addition, the hydrophilic and hydrophobic properties of peptides determined by genes and -helical and -sheet domains are used as musical bifurcations to make music. Each step is as follows.

① Determine the pitch

The pitch is determined by the first two letters of the DNA code. The resulting combination is 4x4 = 16, but 12 notes are assigned to 13 chords except TA, TG, and CC for mode change. In the remaining code, put the appropriate code.

② sound intensity determination

The loudness is determined by the last character of the DNA code.

③ Determine Beat

The time signature is also determined by the first two letters of the DNA code. At this time, apply inversion or retrograde to prevent pitch and beat progressing equally.

④ Aftertreatment

The hydrophilic and hydrophobic properties of the peptides determined by the genes, and -helical and -sheet structures are used as musical branching points to change the tune.

⑤ Instrument decision

Musical instruments belong to preferences, allowing the freedom to choose instruments. In some cases, the instrument can be selected using the DNA characteristics of the individual.

4 is a flowchart illustrating a music generation method using a DNA base sequence and a service providing method using the same.

The DNA sequence (1) is extracted through a commercial DNA analysis method using an existing DNA analyzer (step S1), and the music generation tool (2) is used to convert the DNA sequence (1) into music for each individual. A unique DNA result music file 3 is generated (step S2), and the result music files 3 are accumulated and stored in the service database 4 (step S3).

Distribute the DNA music file to the user who visited the service database 4 via offline (step S4), or transmit the DNA music file to the online user's computer 7 via the web server 6 to the Internet network. (Step S5).

Therefore, DNA sequences having unique characteristics among individuals can be converted into music media to analyze DNA characteristics as music, and the natural sound of DNA itself can be heard, and DNA characteristics unique to individuals can be converted to sounds. It allows you to have your own unique music, and you can create a new field of research that shows how each creature responds by playing its own DNA music.

As described above, since the music generation by the DNA nucleotide sequence and the service providing method using the same according to the present invention convert the genetic information into the media, academically, in the genetic analysis, the music analysis method may be applied in addition to the existing genetic analysis method. You can listen to gene-specific music, accumulate these data, analyze similarities with existing music, study the response of living things when you play DNA music, and apply it to individual genes. When you do that, you can own your own music and provide a means to increase your bond by exchanging your own music with each other.

In addition, by converting the DNA sequence having unique characteristics between individuals into music media, the DNA characteristics can be analyzed as music, and the natural sound of DNA itself can be heard, and the individual's own DNA can be converted to sound It has the effect of opening a new field of research that allows people to have their own unique music and how each creature responds by listening to their DNA music.

Claims (4)

In a system related to sequence extraction through a DNA analyzer: Extracting a DNA sequence through a commercially available DNA analysis method using an existing DNA analyzer (S1); Generating a DNA music file unique to each individual by converting the DNA sequence into music using a DNA music generating tool (S2); Storing the generated DNA music file in a database (S3); And Distributing the music file to the user visited via offline (S4), or transmitting the DNA music file to the computer of the online user via the web server to the Internet network (S5) characterized in that it comprises a Music generation and service providing method using the same. The method of claim 1, Generating the DNA music file DNA sequences are represented by four letters, A, C, G, and T, and these four letters are pairs of three, one code, or T may be represented as U. Create a basic note by substituting the note length and intensity, and apply inversion or retrograde to prevent equal pitch and beat progression. Music generation using DNA sequencing, characterized in that to generate music using the domains as a musical branch and a service providing method using the same. The method of claim 2, Since the pitch determination determines pitch by the first two letters of the DNA code, the resultant combination is 4x4 = 16, but 13 codes except TA, TG, and CC for mode change are used. 12 chords are inserted into the remaining chords and the appropriate chords are inserted. The sound intensity determination determines the intensity with the last character of the DNA code, and the time determination determines the beat with the first two letters of the DNA code, such as the beat and the pitch. Music generation by DNA sequencing characterized in that to apply inversion or retrograde to prevent and service providing method using the same. The method of claim 2, The hydrophilicity, hydrophobicity and -helical, -sheet structure of the peptides determined by the DNA genes are used as musical branching points, and the music is changed. Therefore, the music production by the DNA nucleotide sequence, characterized in that the instrument can be selected using the characteristics of the individual DNA and service providing method using the same.