KR20030010176A

KR20030010176A - Asthma treatment of ginkgo fruit extrat and walnut fruit extrat

Info

Publication number: KR20030010176A
Application number: KR1020010044997A
Authority: KR
Inventors: 이병두
Original assignee: 이병두
Priority date: 2001-07-25
Filing date: 2001-07-25
Publication date: 2003-02-05

Abstract

PURPOSE: A therapeutic agent for asthma containing simultaneously an extract of ginkgo nut and walnut is provided which has a synergic therapeutic effect on asthma. CONSTITUTION: A ginkgo nut and walnut are extracted in alcohol or an organic solvent selected from the group consisting of hexane, ether, ethyl acetate, chloroform and alcohol. The oral administration of an extract of ginkgo nut and walnut in an amount of 0.1mg/ml into a patient who is suffering from asthma two times per a day for 5 days offers a good treating action on asthma.

Description

Asthma treatment of ginkgo fruit extrat and walnut fruit extrat}

본 발명은 은행 열매와 호도 열매의 추출물을 이용한 천식 치료에 관한 것이다.The present invention relates to the treatment of asthma using extracts of ginkgo biloba and hawthorn fruit.

일반적으로 천식이란 갑자기 발작적으로 기관지 수축이 일어나 호흡곤란이 발생하는 질환으로 가역적 기도 폐색,기도의 염증 증가, 기조 반응성 증가 등의 병태생리학적 특징을 나타낸다. 이러한 천식은 기관지 염증에 관여하는 Eosinophil, 비만세포라 불리는 Mast cell 등의 염증세포의 과민 반응을 억제 시키고 이들이 분비하는 염증 매개체인 Histamine, Leukotriene등의 과다 분비를 억제시켜 체내 염증매개반응이 필용이상으로 발생치 못하도록 함이 근본치료 방법이다.In general, asthma is a disease that causes bronchial contraction due to sudden seizures, which causes pathophysiological characteristics such as reversible airway obstruction, increased airway inflammation, and increased basal reactivity. Such asthma inhibits hypersensitivity reactions of inflammatory cells such as Eosinophil and mast cells called mast cells, and inhibits excessive secretion of inflammatory mediators such as Histamine and Leukotriene. Preventing it is a fundamental treatment.

본 발명은 동물실험에서 대조군과 약물실험군간의 현저한 호흡 양상의 변화를 보였고 천식 환자에 대한 투약에서도 유효성을 나타냈다.The present invention showed a significant change in breathing pattern between the control group and the drug test group in the animal experiments and also showed efficacy in the administration to asthma patients.

은행과 호도는 고래로 한약재와 식품으로 널리 사용되어 왔으나 은행과 호도의 익지 않은 열매는 사용되지 않았었다. 본 발명은 지금까지 사용하지 않았던 그 열매를 이용한 천식 치료제 개발이다.Ginkgo and Hodo have been widely used as herbal medicine and food as whales, but the raw fruits of Ginkgo and Hodo have not been used. The present invention is to develop an asthma treatment using the fruit that has not been used until now.

본 발명은 은행 열매 추출물과 호도 열매 추출물을 동시 또는 각각 사용하였을 때 천식에 탁월한 효과가 있음을 발견하고 그 추출 방법과 유효한 천식 치료제 개발을 목적으로 한다.The present invention has been found to have an excellent effect on asthma when the ginkgo fruit extract and the hoedo fruit extract are used simultaneously or separately, and the object of the present invention is to develop the method and effective treatment for asthma.

본 발명은 은행 열매 추출물과 호도 열매 추출물을 동시에 또는 각각 사용하여 천식 치료제를 개발함을 그 특징으로 한다.The present invention is characterized in that ginkgo biloba extract and fenugreek fruit extract are used simultaneously or separately to develop an asthma treatment.

본 개발에 사용된 은행과 호도는 과거 그 씨앗이 한약재와 식품으로 널리 사용되어 왔으나 본 개발에서 사용된 것은 그 씨앗을 사용하기 이전인 4월경부터 8월경에 채집하여 이를 각각, 또는 동시에 반량씩 섞어 이를 적당량으로 분쇄 후 알코올로 추출하거나 알코올추출물에 증류수로 현탁하고 유기용매인 헥산, 에테르, 에틸아세테이트, 클로로포름, 알코올을 사용하여 추출 할 수 있다. 또는 은행 열매나 호도 열매를 물로 환류 추출하여 그 여액을 감압농축하여 사용할 수 있다.In the past, the ginseng and hodo used in this development have been widely used as herbs and foods in the past, but in this development, they were collected from April to August, before the seeds were used, and then mixed in half or at the same time. This may be crushed to an appropriate amount and extracted with alcohol or suspended in distilled water in alcohol extracts and extracted using organic solvents hexane, ether, ethyl acetate, chloroform and alcohol. Alternatively, ginkgo biloba or hawthorn fruit can be extracted under reflux with water, and the filtrate can be concentrated under reduced pressure.

이하 실시예에 의하여 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail with reference to Examples.

단, 하기 실시예들은 본 발명을 예시하는 것으로 본 발명의 내용이 실시예에 의하여 한정되는 것은 아니다.However, the following examples are illustrative of the present invention, and the content of the present invention is not limited by the examples.

〈실시예1〉 실험동물에서의 호흡변화Example 1 Respiratory Changes in Experimental Animals

실험동물을 체중250g내외의 수컷흰쥐 20마리를 사용하여 2주간 적응기간후 사용하였다.Experimental animals were used after two weeks of adaptation using 20 male rats of about 250 g body weight.

Egg white을 100℃에서 30분간 중탕한 후 정제수에 잘 세정하고 100%알코올에서 48시간동안 탈수한 다음 4*4*10mm의 절편을 만든다. 흰쥐를 3.5% chloral hydrate(35㎎/100g)로 마취한 후 egg white을 복부 피하에 이식시키고 피부를 봉합했다. egg white감작후 3%egg albumin을 기화시켜 10분간 기도를 통하여 천식을 유발시킨다.Egg white was soaked at 100 ° C for 30 minutes, washed well in purified water, dehydrated in 100% alcohol for 48 hours, and then made into 4 * 4 * 10mm sections. Rats were anesthetized with 3.5% chloral hydrate (35mg / 100g), and then egg white was implanted subcutaneously and sutured. After sensitizing egg white, 3% egg albumin is vaporized to induce asthma through airway for 10 minutes.

천식유발 후 실험동물을 대조군, 은행추출군, 호도추출군, 혼합추출군으로 나누고 호흡감지장치가 부착된 마스크를 흰쥐의 두경부에 장착한 다음 physiograph로 호흡변화를 관찰했다. 이때 각각의 추출물은 0.1㎎/㎖를 5㎖경구 투여하였다.After asthma induction, the experimental animals were divided into control group, bank extract group, hoe extract group, and mixed extract group. A mask with a breathing apparatus was attached to the head and neck of the rat, and the change of breath was observed by physiograph. At this time, each extract was administered orally 0.1mg / ㎖ 5ml.

위 실험에서 대조군은 일시적인 호흡의 정지가 나타나거나 호흡의 흡기가 연장되었고 나머지 실험군에서는 모두 자연스러운 호흡상황을 보여주었다.In the above experiments, the control group showed temporary stopping of breathing or prolonged breathing, and the rest of the experiments showed natural breathing conditions.

〈실시예2〉 실험동물기관조직의 호산구 침윤 관찰<Example 2> Observation of eosinophil infiltration of experimental animal organ tissue

실시예1의 실험동물을 전신마취 시킨 후 개흉하여 후두 약2mm아래에서 기관조직을 채취한 다음 10% 포름알린 고정액에 24시간 고정한 조직을 파라핀 포매 과정을 거쳐 8㎛두께로 조직절편을 만들었다. 이 조직절편을 hematocycline and eosin염색을 하고 광학현미경하에서 기관조직내 호산구수를 세었다.After the general anesthesia of the experimental animal of Example 1, the thoracic thorax was collected from about 2 mm below the larynx, and the tissues fixed in 10% formally fixed fixative for 24 hours were paraffin-embedded. The tissue sections were hematocycline and eosin stained and counted in the tracheal eosinophils under light microscopy.

〈실시예3〉 자원자에 대한 투약 예후Example 3 Prognosis of Volunteers

각각의 추출물을0.1㎎/㎖농도로 천식 질환이 있는 자원자에게 100㎖씩 1일 2회 아침, 저녁 공복시 5일간 복용케 후 그 예후를 관찰하였다Each extract was administered to volunteers with asthma disease at a concentration of 0.1mg / ml twice a day, morning and evening, on an empty stomach for 5 days and then the prognosis was observed.

상기에서 살펴본 바와 같이, 은행 열매의 추출물과 호도 열매의 추출물은 우수한 천식치료작용을 나타냈으며 혼합투여시는 그 효과가 더욱 우수하여 천식치료제로 유용허게 사용될 수 있다.As described above, the extract of Ginkgo biloba and the extract of Hodo fruit showed an excellent asthma treatment, and when mixed administration, the effect is more excellent and can be usefully used as an asthma treatment.

Claims

Asthma remedy using extract of ginkgo biloba as active ingredient

Asthma medicines based on extracts of hawthorn berries

Ginkgo biloba extract with anti-asthma effect obtained by extracting ginkgo biloba with alcohol or suspended in distilled water and extracted with one or more organic solvents selected from hexane, ether, ethyl acetate, chloroform and alcohol.

Ginkgo biloba extract with anti-asthma effect obtained by extracting Hodo fruit with alcohol or suspended in distilled water and extracting hexane, ether, ethyl acetate, chloroform and alcohol with one or more organic solvents selected from