KR20020066060A - Composition of microbicide for disinfecting a toothbrush, Method for manufacturing the composition, and Method for disinfecting a toothbrush using the composition - Google Patents

Abstract

PURPOSE: A disinfection composition capable of sanitarily storing a toothbrush with quick and sustained sterilizing power by simply spraying 2 to 3 times or dipping in the disinfection composition or diluted solution thereof is provided which prevents infectious disease such as a cold as well as oral disease such as peridontal disease and bad breath. CONSTITUTION: This disinfection composition contains: 20 to 70% by weight of ethanol for quick-acting disinfection; 0.01 to 2.0% by weight of trichloro acid for slow-acting disinfection; 0.01 to 1.0% by weight of sodium benzoate for reinforcing the disinfecting action; additionally 1.0 to 30.0% by weight of concentrated glycerin for sustainedly reinforcing the disinfecting action and preventing bursting during the winter; and 0.01 to 1.0% by weight of a perfume, 0.0001 to 0.1% by weight of a dye and 0.001 to 0.5% by weight of a stabilizer such as 2Na-EDTA.

Description

Composition of microbicide for disinfecting a toothbrush, Method for manufacturing the composition, and Method for disinfecting a toothbrush using the composition}

The present invention relates to a disinfectant composition, a method for producing the same, and a method for sterilizing a toothbrush using the same. More specifically, the present invention relates to a disinfectant composition for sterilization of a toothbrush and a method for producing the same.

Until now, little efforts have been made to keep the toothbrush, a personal hygiene product used by everyone for the health of the oral cavity, free of germs. Investigating how the average toothbrush user cares for their toothbrush shows that most users rinse with water after brushing and store it with several toothbrushes in a container such as a cup. there was. However, in general, about 300 kinds of bacteria exist in the oral cavity of a person. For example, the causative agents of periodontal diseases such as Treponema denticola, Fusobacterium, Selenomona, Peptococcus, and the like, bad breath causing bacteria such as Porphyromonas, Prevotella, Haemophilus, and Veillonella . In addition, infectious bacteria or pathogenic microorganisms may be present in the oral cavity. When these bacteria and the like adhere to the toothbrush during brushing, and if several toothbrushes are stored together in one container such as a cup, there is a high possibility that the bacteria cross-contaminate each other between the toothbrushes. Therefore, due to the wrong storage method of the toothbrush, not only oral diseases such as periodontal disease, bad breath, but also the risk of transmission of infectious diseases such as cold.

In order to solve the above problems, at home, the toothbrush is boiled in boiling water, or used to sterilize in the sun. However, since the toothbrush is contaminated with bacteria again with one use, it was practically impossible to perform the above method every time after brushing.

In addition, in order to keep the toothbrush hygienic, an electric device such as an ozone generating toothbrush sterilizer or a UV generating toothbrush sterilizer was connected to a power source. However, these were expensive and were a big burden for the general consumer to use. In addition, there is a risk of electric shock because electrical appliances must be installed in a humid bathroom.

The present invention is to solve the above problems, the object of the present invention is to rinse the toothbrush in water after brushing, simply by spraying two or three times a fungicide composition harmless to the human body or simply immersed in the fungicide composition or diluent thereof Providing a disinfectant composition, a method of manufacturing the same, and a method of disinfecting a toothbrush using the same, which prevents the spread of infectious diseases such as periodontal disease, bad breath, or other diseases such as periodontal disease, bad breath, and the like by storing the toothbrush hygienically with fast and continuous sterilization It is.

1a and 1b are photographs showing the results of treatment of 25 μl and 50 μl of the fungicide composition according to Example 1 of the present invention to culture of S. aureus .

2A and 2B are photographs showing the results of treatment of 25 μl and 50 μl of the fungicide composition according to Example 1 of the present invention to the culture of E. coli .

3a and 3b are photographs showing the results of treatment of 25 μl and 50 μl of the fungicide composition according to Example 1 of the present invention to the culture of Pseudomonas .

4A and 4B are photographs showing the results of treatment of 25 μl and 50 μl of the fungicide composition according to Example 1 of the present invention to culture of C. albicans .

5A and 5B are photographs showing the results of treatment of 25 μl and 50 μl of the fungicide composition according to Example 1 of the present invention to the culture of A. niger .

6a and 6b are photographs showing the results of treatment of 25 μl and 50 μl of the fungicide composition according to Example 2 of the present invention to the culture of S. aureus .

7a and 7b are photographs showing the results of treatment of 25 μl and 50 μl of the fungicide composition according to Example 2 of the present invention to the culture of E. coli .

8a and 8b are photographs showing the results of treatment of 25 μl and 50 μl of the fungicide composition according to Example 2 of the present invention to the culture of Pseudomonas .

9a and 9b are photographs showing the results of treatment of 25 μl and 50 μl of the fungicide composition according to Example 2 of the present invention to culture of C. albicans .

10A and 10B are photographs showing the results of treatment of 25 μl and 50 μl of the fungicide composition according to Example 2 of the present invention to the culture of A. niger .

11A to 14C are photographs showing the degree of contamination by microorganisms after the toothbrushes were treated with the fungicide composition according to the present invention after 5 minutes, 4 hours and 8 hours, respectively, after brushing teeth.

In order to achieve the above object, the disinfectant composition according to the present invention is 20 to 70% by weight of ethanol for fast-acting sterilization as volatile; 0.01 to 2.0% by weight of trichloroacid for sustained sterilization; And 0.01 to 1.0% by weight sodium benzoate to enhance the bactericidal action.

The fungicide composition is characterized in that it further comprises 1.0 to 30.0% by weight of concentrated glycerin to continuously enhance the bactericidal action and prevent freezing.

The fungicide composition is characterized in that it further comprises 0.01 to 1.0% by weight of fragrance, 0.0001 to 0.1% by weight dye and 0.001 to 0.5% by weight stabilizer.

In the fungicide composition, the stabilizer is characterized in that 2Na-EDTA.

In addition, in order to achieve the above object, the preparation method of the disinfectant composition according to the present invention, 20 to 70% by weight of ethanol and 0.01 to 1.0% by weight of sodium benzoate in a first melting tank mixing step of mixing; A second mixing step of adding 0.01 to 2.0% by weight of trichloro acid together with water into a second melting tank and mixing and stirring; And a third mixing step of mixing and stirring the mixture formed in the first mixing step and the mixture formed in the second mixing step.

In the method for producing a disinfectant composition, the second mixing step is characterized in that the mixture is stirred by adding 1.0 to 30.0% by weight of concentrated glycerin to the second dissolution tank.

In the method for preparing the disinfectant composition, the second mixing step, 0.01 to 1.0% by weight of the fragrance, 0.0001 to 0.1% by weight of the dye and 0.001 to 0.5% by weight of the stabilizer is characterized in that the mixture is further mixed. do.

In addition, in order to solve the above problems, the toothbrush sterilization method according to the present invention is characterized in that, after brushing, spraying the disinfectant composition according to the present invention, the toothbrush.

In addition, the toothbrush sterilization method according to the present invention is characterized in that after brushing, the toothbrush is immersed in the disinfectant composition according to the present invention.

In the method of sterilizing the toothbrush according to the present invention, the disinfectant composition is characterized in that it is diluted on a separate container immediately before being used in the toothbrush after brushing.

Hereinafter, the sterilant composition according to the present invention, a manufacturing method thereof, and a method of sterilizing a toothbrush using the same will be described in detail.

The fungicide composition according to the present invention contains ethanol and trichloro acid as main disinfectants. Ethanol is volatile and has a fast-acting bactericidal effect. Therefore, it acts on microorganisms in a short time and shows a wide range of bactericidal effects. For this reason, ethanol is widely used as a fungicide in other fields. Among the ethanol, the most sterilizing power is known to be about 75% ethanol. However, since the fungicide of the present invention is used in a toothbrush directly applied to the mouth, it has an ethanol content lower than 75% in order to reduce the peculiar smell of alcohol, but may exhibit excellent sterilizing power even with a low ethanol content. In order to achieve the object of the present invention, the ethanol is preferably fermented ethanol.

Another main bactericide contained in the fungicide composition of the present invention is trichloro acid. Trichloro acid here refers to "2, 4, 4-trichloro-2-hydroxy- diphenyl ether". Unlike ethanol, it has a sustained bactericidal effect. Trichloric acid is a function that maintains sterilization power continuously until the next toothbrush use, because even after the bacteria are removed by the instantaneous strong sterilization effect caused by ethanol, the bacteria are likely to be infected again from the surrounding toothbrush or the air. Do it.

In addition to the fungicides, the fungicide composition of the present invention contains sodium benzoate. Sodium benzoate has the effect of enhancing the bactericidal power of the ethanol and trichloro acid. Sodium benzoate is excellent for human safety and is therefore suitable for disinfecting toothbrushes that come in direct contact with the mouth.

It is preferable that glycerin is further included in addition to the said sodium benzoate. Glycerin is also a safe substance. Glycerin has a relatively low volatility compared to ethanol, the main disinfectant, thus providing a continuous sterilization function. In addition, it enables freeze protection at low temperatures. In order to satisfy the function of freezing prevention, it is usually preferred to use at least about 4% by weight of the total composition. In order to achieve the object of the present invention, the glycerol is preferably about 95.0% or more concentrated glycerol.

In order to sterilize a toothbrush according to the present invention, it is preferable to spray the sterilant composition according to the present invention to the toothbrush or to immerse the sterilant composition. To spray on a toothbrush, the disinfectant composition according to the present invention is stored in a transparent or opaque container of 5 to 300 ml of a mist pump or a spray gun type. The stored fungicide composition can be seen by excellent spraying effect by simply spraying when used after brushing. Otherwise, the toothbrush may be sterilized by direct soaking in the fungicide composition according to the present invention. After brushing the disinfectant composition stored in a 5 to 300 ml transparent or opaque container, the toothbrush may be directly immersed in a small amount, or diluted slightly in a separate container, and then immersed in the diluent. At this time, it is preferable that the amount to be removed once used is 1 to 20ml.

Hereinafter, the inventors conducted the following experiment to prove the effect of the fungicide composition according to the present invention.

Example

Example 1

45% by weight of fermented ethanol and 0.5% by weight of sodium benzoate were added to the first melting tank, followed by mixing and stirring at a strength of 300 rpm using a stand AGI MIXER. 54.079% by weight of purified water, 0.2% by weight of trichloroic acid, 0.2% by weight of peppermint, 0.001% by weight of dye and 0.02% by weight of 2Na-EDTA were sequentially added to a second melting bath, which is separate from the dissolution tank, and then stands. The mixture was stirred at an intensity of 300 rpm using an AGI MIXER. Thereafter, the contents of the second dissolution tank were introduced into the first dissolution tank, followed by stirring for 10 minutes at a strength of 300 rpm with a stand AGI MIXER to obtain a sterilizing agent composition according to the present invention.

In order to demonstrate the bactericidal effect of the fungicide composition according to the present invention obtained in Example 1, a hollow zone test was performed on the test specimen OXO0109-GA in the following manner.

1. Strains to be tested

(1) Bacteria: Staphylococcus aureus KCTC 1916

Escherichia coli KCTC 2571

Pseudomonas KCTC 2513

(2) Yeast: Candida albicans KCTC 7965

(3) Mold: Aspergillus niger (black mold) KCTC 6960

2. Experiment Method

According to KS K 0692-1984 (Hallow zone Test) according to the antimicrobial test of the antimicrobial processed fabrics, the antimicrobial and bactericidal tests were carried out in the following steps.

1) Staphylococcus aureus KCTC 1916, Escherichia coli KCTC 2571, Pseudomonas KCTC 2513 and Candida albicans KCTC 7965 were incubated overnight in a liquid medium and counted with a hemocytometer. The number was set to 10 ⁵ .

2) After culturing Aspergillus niger (black mold) KCTC 6960 in a solid medium, the spores were scraped and placed in sterile distilled water, filtered with gauze and counted by the method of 1) to obtain a spore solution of 10 ⁵ / ml. Prepared.

3) The bacteria, yeast and mold were evenly spread on a solid medium using a sterile swab. The number of microorganisms inoculated was as follows.

Staphylococcus aureus KCTC 1916: 11.0 × 10 ⁵ cfu / ml

Escherichia coli KCTC 2571: 1.68 × 10 ⁶ cfu / ml

Pseudomonas KCTC 2513: 9.65 × 10 ⁵ cfu / ml

Candida albicans KCTC 7965: 6.73 × 10 ⁵ cfu / ml

Aspergillus niger (black mold) KCTC 6960: 7.00 × 10 ⁵ cfu / ml

4) A test piece, a 0.8 mm diameter paper disc, is placed in the center of the Petri-Dish containing the solid medium coated with bacteria, yeast and mold, and 25 μl and 50 μl, respectively, of the fungicide composition solution according to the present invention on the paper disc. Was treated.

5) After the treatment, the size of the halo formed by inhibiting the growth of the bacteria, yeast and mold by the fungicide composition according to the present invention was measured. The size of growth-lowering ring was measured as follows.

W = T-D / 2

W: Part of the actual germ, yeast and mold growth (actual growth zone) (mm)

T: diameter of the entire growth-lowering ring including the test piece (mm)

D: diameter of test piece (mm)

the results are as follow.

Strain Size of growth zone (mm) 25μl fungicide 50μl fungicide One Staphylococcus aureus (Staphylococcus aureus) 25.2 31.8 2 Escherichia coli (E. coli) 13.2 13.8 3 Pseudomonas 3.80 4.00 4 Candida albicans 1.80 7.70 5 Aspergillus niger (black mold) 6.50 7.00

As shown in Table 1, the fungicide composition according to the present invention was found to have an excellent bactericidal effect against bacteria, particularly Staphylococcus aureus ( E. coli ) and Escherichia coli (E. coli). In addition, Candida albicans (Candida) and Aspergillus niger (black mold) were found to have an excellent bactericidal effect. Therefore, the bactericidal composition of the present invention has been proved to have an excellent bactericidal effect on not only bacteria but also yeast and mold. The bactericidal effect has been shown to rise with increasing concentration of the bactericide composition. In all the strains, the bactericidal effect was better when the concentration of the bactericide was 50 µl than when the concentration of the bactericide was 25 µl. The results of the first embodiment are also shown in the photographs of Figs. 1 to 5 show a round ring formed by killing and not growing microorganisms around the test piece treated with the sterilizing agent.

Example 2

40% by weight of fermented ethanol and 0.5% by weight of sodium benzoate were added to the first melting tank, followed by mixing and stirring at a strength of 300 rpm using a stand AGI MIXER. And 48.779% by weight of purified water, 10.0% by weight of concentrated glycerin (95.0%), 0.5% by weight of trichloroic acid, 0.2% by weight of peppermint fragrance, 0.001% by weight of dye and 0.02% by weight of 2Na-EDTA and another separate from the dissolution tank The solution was sequentially added to a second melting tank, followed by stirring at a strength of 300 rpm using a stand AGI MIXER. Thereafter, the contents of the second dissolution tank were introduced into the first dissolution tank, followed by stirring for 10 minutes at a strength of 300 rpm with a stand AGI MIXER to obtain a sterilizing agent composition according to the present invention.

With the obtained disinfectant composition according to the present invention, a hollow zone test was performed on the experimental specimen OXO0109-GB in the same manner as in Example 1.

The results were as follows.

Strain Size of growth zone (mm) 25μl fungicide 50μl fungicide One Staphylococcus aureus (Staphylococcus aureus) 26.0 34.3 2 Escherichia coli (E. coli) 15.7 17.7 3 Pseudomonas 4.20 5.00 4 Candida albicans 6.50 8.50 5 Aspergillus niger (black mold) 6.50 8.50

As shown in Table 2, the fungicide composition prepared according to Example 2 was found to have an excellent bactericidal effect against bacteria, in particular Staphylococcus aureus ( E. coli ) and Escherichia coli (E. coli). In addition, Candida albicans (Candida) and Aspergillus niger (black mold) were found to have an excellent bactericidal effect. Therefore, the bactericidal composition of the present invention has been proved to have an excellent bactericidal effect on not only bacteria but also yeast and mold. The bactericidal effect has been shown to rise with increasing concentration of the bactericide composition. In all the strains, the bactericidal effect was better when the concentration of the bactericide was 50 µl than when the concentration of the bactericide was 25 µl.

The results of the second embodiment are also shown in the photographs of Figs. These pictures also show a round ring formed by killing microorganisms without killing the test specimen treated with the fungicide composition according to the present invention.

In addition, it can be seen that the composition of Example 2 has a better bactericidal effect on all the microorganisms than the composition of Example 1 above. As described above, Example 2 shows about 5 wt% less fermentation ethanol than Example 1, which shows a better effect: an increase of 0.3 wt% of trichloroic acid and 10 wt% of concentrated glycerin (95.0 wt%). It can be seen that the result is the addition of%. Therefore, the fungicide composition according to the present invention was found to include the most preferable fermentation ethanol, trichloro acid and glycerin.

In addition to the above experiments, the present inventors performed the following experiments in order to prove the effect of applying the fungicide composition prepared in Example 2 to the toothbrush actually used.

First, four toothbrushes were given to four persons (A, B, D, and D) who did not have any special pathological diseases in the oral cavity. One toothbrush of each of the four toothbrushes of each experimenter used as above was kept untreated as a control. The remaining three toothbrushes were sprayed five times (about 1 cc) with the disinfectant composition prepared in Example 2, and then 5 minutes (Experimental Group 1), 4 hours (Experimental Group 2) and 8 hours (Experimental Group 3) were passed. Thereafter, each of the four toothbrushes of each experimenter was put in a cap tube containing 20 ml of saline solution, and shaken sufficiently. Thereafter, 1 ml of the solution in the cap tube was serially diluted in 9 ml of saline solution, and 0.1 ml of the solution was plated three times in a solid medium in dilution steps and incubated in the incubator for about 24 to about 48 hours. Thereafter, the number of viable cells was counted for the control group and the experimental group 1. The results are shown in Table 3 below.

experimenter Sterilizer treatment Bacteria Mortality end Control 4.1 × 10 ³ 0% Experimental Group 1 0 100% I Control 4.1 × 10 ³ 0% Experimental Group 1 0 100% All Control 4.1 × 10 ³ 0% Experimental Group 1 0 100% la Control 4.1 × 10 ³ 0% Experimental Group 1 0 100%

As shown in Table 3, when the fungicide composition according to the present invention was treated, it was found that in all four people, 100% of the bactericidal effect was shown within 5 minutes after the treatment of the fungicide composition according to Example 2. Of course, there may be some differences depending on the individual, but the infections such as bacteria that occur between normal normal people were able to confirm the excellent effect to prevent 100%. Results for Experimental Group 2 and Experimental Group 3 not shown in Table 3 are shown in the photographs in FIGS. As shown in Figures 11 to 14, it was found that even after 5 minutes, 4 hours, and even 8 hours after treatment with the fungicide composition according to the present invention, the microorganism hardly proliferated. Therefore, the treatment of the fungicide composition according to the present invention was found to have a sterilizing effect lasting more than 8 hours as well as the instant bactericidal power in which 100% of bacteria were sterilized within 5 minutes after the treatment. As such, since the bactericidal effect lasts until the next tooth brushing, it has been proved that a good bactericidal effect can be obtained by spraying or immersing the fungicide composition according to the present invention after brushing the teeth.

By using the disinfectant composition and the method for preparing the same according to the present invention, after brushing the teeth, the toothbrush can be rinsed in water and sanitized with a quick and continuous disinfecting power by simply spraying the disinfectant composition harmless to the human body several times or immersing it in the disinfectant composition or diluent thereof. Because it can be stored as, it is possible to prevent the spread of infectious diseases such as colds, as well as oral diseases such as periodontal disease, bad breath.

Claims (11)

20 to 70% by weight of ethanol for fast-acting sterilization as volatile; 0.01 to 2.0% by weight of trichloroacid for sustained sterilization; And Disinfectant composition for sterilization of the toothbrush, characterized in that it comprises 0.01 to 1.0% by weight sodium benzoate to enhance the bactericidal action. According to claim 1, Sterilizer composition for sterilization of the toothbrush, characterized in that it further comprises 1.0 to 30.0% by weight of concentrated glycerin to continuously enhance the bactericidal action and prevent freezing. The disinfectant composition of claim 1 or 2, further comprising 0.01 to 1.0% by weight of fragrance, 0.0001 to 0.1% by weight of dye, and 0.001 to 0.5% by weight of stabilizer. According to claim 3, wherein the stabilizer is a disinfectant composition for sterilization of the toothbrush, characterized in that 2Na-EDTA. A first mixing step of mixing 20 to 70% by weight of ethanol and 0.01 to 1.0% by weight of sodium benzoate in a first melting tank and mixing and stirring; A second mixing step of adding 0.01 to 2.0% by weight of trichloro acid together with water into a second melting bath and mixing and stirring; And And a third mixing step of mixing and stirring the mixture formed in the first mixing step and the mixture formed in the second mixing step. The method of claim 5, wherein the second mixing step, Method of producing a disinfectant composition for sterilization of the toothbrush, characterized in that the mixture further stirred by adding 1.0 to 30.0% by weight of concentrated glycerin to the second melting tank. The method of claim 6, wherein the second mixing step, 0.01 to 1.0% by weight of the fragrance, 0.0001 to 0.1% by weight of the dye and 0.001 to 0.5% by weight of the stabilizer is added to the method for producing a sterilant composition for the sterilization of the toothbrush. After brushing, the disinfectant composition according to any one of claims 1 to 4 is sprayed onto the toothbrush. After brushing, the toothbrush is sterilized, wherein the toothbrush is immersed in the disinfectant composition according to any one of claims 1 to 4. 10. The method of claim 9, wherein the disinfectant composition is diluted on a separate container immediately after being brushed and used for the toothbrush. After brushing, the disinfectant composition according to any one of claims 1 to 4 is filled into an aerosol-type spray container and sprayed.