KR20010110272A - Glucose Electrode Containing Pt metal and its efficiency - Google Patents

Glucose Electrode Containing Pt metal and its efficiency Download PDF

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Publication number
KR20010110272A
KR20010110272A KR1020010072488A KR20010072488A KR20010110272A KR 20010110272 A KR20010110272 A KR 20010110272A KR 1020010072488 A KR1020010072488 A KR 1020010072488A KR 20010072488 A KR20010072488 A KR 20010072488A KR 20010110272 A KR20010110272 A KR 20010110272A
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South Korea
Prior art keywords
electrode
glucose
ink
analyzing
platinum metal
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KR1020010072488A
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Korean (ko)
Inventor
이동훈
이기자
송지혜
정홍식
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홍영표
(주)바이오첵
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Priority to KR1020010072488A priority Critical patent/KR20010110272A/en
Publication of KR20010110272A publication Critical patent/KR20010110272A/en
Priority to KR10-2002-0034937A priority patent/KR100453483B1/en
Priority to KR2020020028684U priority patent/KR200297594Y1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers

Abstract

PURPOSE: An electrode for analyzing glucose containing platinum metal is provided to improve analyzing performance by forming electrode through platinum metal ink by simple screen printing method and to detect improved electrode performance caused by change of curing temperature. CONSTITUTION: An electrode for analyzing glucose forms an ink containing platinum metal by a screen-printing method. The electrode is formed by mixing carbon ink into a platinum metal ink to increase safety and analyzing performance of the glucose of the electrode. The glucose is analyzed by using an electric chemical method through ferment response. The glucose-analyzing test is operated by regular voltage of 400mV. Moreover, the glucose-analyzing test uses Ag/AgCl as a reference electrode and carbon as relative electrode. The reference and relative electrode is formed by ink of the screen-printing method. The carbon ink is mixed into the platinum ink to prevent noise despite dropping analyzing performance of the glucose than the electrode of the platinum metal ink. Thereby, the electrode for analyzing the glucose improves analyzing performance by forming the electrode through platinum metal ink by simple screen-printing method.

Description

Pt 금속을 함유한 글루코스 분석용 전극과 성능{Glucose Electrode Containing Pt metal and its efficiency}Glucose Electrode Containing Pt metal and its efficiency

본 발명은 Pt 금속 잉크를 이용하여 글루코스 분석을 효과적으로 할 수 있는 전극의 성능에 관한 것이다.The present invention relates to the performance of an electrode capable of effective glucose analysis using Pt metal ink.

기존의 글루코스 분석은 Pt 금속을 가공하여 전극으로 직접 사용하는 방법이 널리 이용되어 왔다. 하지만 Pt 금속 전극 자체는 내구성이 있지만 가격이 너무 비싸고 가공이 어렵기 때문에 바이오센서나 여러 가지 응용을 위한 공정에는 어려움이 있었다.Conventional glucose analysis has been widely used to process Pt metal and use it directly as an electrode. However, because Pt metal electrodes themselves are durable, they are too expensive and difficult to process, making them difficult for biosensors and other applications.

본 발명의 목적은 가공이 간편한 스크린 프린팅 방법을 통하여 Pt 금속 잉크로 전극을 만들어 글루코스 분석 성능을 높이고자 한다. 따라서 curing 온도 변화에 기인된 전극 성능의 향상을 알아보고 또한 전기적으로 안정한 탄소(C) 잉크와 Pt 금속 잉크를 일정한 비율로 섞어 만든 전극의 글루코스 분석 성능을 알아보고자 한다.An object of the present invention is to improve the glucose analysis performance by making an electrode with Pt metal ink through a screen printing method that is easy to process. Therefore, the purpose of this study is to investigate the improvement of electrode performance due to the change of curing temperature and the glucose analysis performance of the electrode made by mixing a certain ratio of electrically stable carbon (C) ink and Pt metal ink.

도 1는 Pt 금속 잉크와 탄소(C)잉크의 조성 비율에 따라 각각 만들어진 전극의 글루코스에 의한 전류변화값을 나타낸 그래프이다.1 is a graph showing a current change value due to glucose of an electrode made according to a composition ratio of a Pt metal ink and a carbon (C) ink.

도 2은 전극의 curing 온도에 의한 글루코스에 의한 전류변화값을 나타낸 그래프이다.Figure 2 is a graph showing the current change value due to glucose by the curing temperature of the electrode.

도 3은 삼상 전극계과 효소 반응식을 나타낸 그림이다3 is a diagram showing a three-phase electrode system and the enzyme reaction scheme

글루코스 분석을 위한 전극은 Pt 금속이 함유된 잉크를 스크린 프린팅 방법을 통해 만들 수 있었다. 전극의 글루코스 분석 성능과 안정도를 높이고자 Pt 금속 잉크에 탄소(C)잉크를 일정량을 섞어 각각의 비율대로 전극을 만들었다. 효소 반응을 이용한 전기화학적 방법을 사용하여 글루코스의 분석 성능을 실험하였다. 실험은 일정 전압 400mV를 사용하여 진행되었으며 삼상 전극계로 기준 전극으로는 Ag/AgCl을, 상대전극으로는 탄소(C)을 사용하였다. 기준 전극과 상대전극 역시 잉크를 가지고 스크린 프린팅으로 만든 전극을 사용하였다.Electrodes for glucose analysis could be made by screen printing ink containing Pt metal. In order to improve the glucose analysis performance and stability of the electrode, a certain amount of carbon (C) ink was mixed with Pt metal ink to make electrodes in each ratio. The analytical performance of glucose was tested using an electrochemical method using an enzymatic reaction. The experiment was conducted using a constant voltage of 400mV. Ag / AgCl was used as the reference electrode and carbon (C) was used as the reference electrode for the three-phase electrode system. The reference electrode and the counter electrode also used an electrode made of screen printing with ink.

Pt 금속 잉크에 탄소(C)잉크를 혼합함으로서 얻는 장점은 Pt 금속 잉크 자체의 전극보다 글루코스 분석 성능은 저하되겠지만 noise 문제 즉 글루코스 분석시 나오는 출력전류값의 안정성과 경제적인 측면이라고 할 수 있겠다.The advantage of mixing carbon (C) ink with Pt metal ink is that the performance of glucose analysis will be lower than that of the electrode of Pt metal ink itself.

이하 첨부 도면과 실시예에 의해 본 발명을 상세히 설명하며, 이들 도면 및 실시예는 오로지 본 발명을 설명하기 위한 것으로 이들에 의해 본 발명의 범위가 한정되지 않는 것은 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자에게는 자명할 것이다.DETAILED DESCRIPTION Hereinafter, the present invention will be described in detail with reference to the accompanying drawings and examples, and these drawings and examples are only for explaining the present invention, and the scope of the present invention is not limited thereto. It will be obvious to those who have knowledge.

도 1는 Pt 금속 잉크와 탄소(C)잉크의 조성 비율에 따른 글루코스에 의한 전류변화값을 나타낸 그래프이다.1 is a graph showing a current change value by glucose according to a composition ratio of Pt metal ink and carbon (C) ink.

도 2은 curing 온도에 의한 글루코스에 의한 전류변화값을 나타낸 그래프이다.2 is a graph showing the current change value due to glucose by curing temperature.

도 3은 삼상 전극계과 효소 반응식을 나타낸 그림이다.3 is a diagram showing a three-phase electrode system and the enzyme reaction scheme.

〈실시예 1〉전극의 글루코스 분석 성능과 noise, curing 시간Example 1 Glucose Analysis Performance, Noise, and Curing Time of an Electrode

표 1은 Pt 금속 잉크와 탄소(C) 잉크의 조성 비율에 따라 측정된 글루코스의 전류변화값과 전극의 안정도이다. 일정전위공급기(Potentiostat)를 사용하여 일정양의 글루코스가 GOD (Glucose Oxidase)와 반응하여 발생하는 과산화수소에 의한 전류변화를 보다 효과적으로 감지하는 전극을 보여주고 있다. 또한 전극의 안정도를 background 전류의 noise 크기로 나타내고 있다. Pt/C 95/5가 가장 좋은 글루코스 분석 성능을 보여주고 있다. 또한 탄소(C) 잉크가 포함됨으로써 출력전류값의 안정성이 향상되었다.Table 1 shows the current variation of glucose and the stability of the electrode measured according to the composition ratio of Pt metal ink and carbon (C) ink. Using a potentiostat, an electrode is used to more effectively detect the current change caused by hydrogen peroxide caused by a certain amount of glucose in response to GOD (Glucose Oxidase). In addition, the stability of the electrode is represented by the noise level of the background current. Pt / C 95/5 shows the best glucose assay performance. In addition, the inclusion of carbon (C) ink improved the stability of the output current value.

표 2는 본 발명에서 사용한 전극을 바이오 센서로서 이용할 때 글루코스와 같이 작용하는 간섭 물질 즉 ascorbic acid(AA), acetamidophenol(AP), uric acid(UA)을 첨가할 때 나오는 전류값을 글루코스에 의한 전류변화값으로 나누어 백분율(%)로 나타낸 것이다.Table 2 shows the current value of glucose when the electrode used in the present invention is added as an interfering substance that acts like glucose, namely ascorbic acid (AA), acetamidophenol (AP), and uric acid (UA). Divided by the change value expressed as a percentage.

표 3은 전극의 전처리에 필요한 curing 시간이 글루코스 분석 성능에 미치는 영향을 알아보았다. Pt 금속 잉크와 탄소(C) 잉크가 동량 섞인 전극으로 실험한 값이다. curing 온도는 130℃이다.Table 3 examines the effect of curing time required on electrode pretreatment on glucose assay performance. It is the value tested by the electrode which mixed same amount of Pt metal ink and carbon (C) ink. The curing temperature is 130 ° C.

본 발명을 이상에서와 같이 상세히 설명하고 입증하였듯이 스크린 프린팅을 이용한 Pt 금속 잉크 전극은 탄소(C) 잉크가 일정량 함유되었을 때 글루코스 분석성능과 안정도가 좋은 것으로 나타내어졌다. 이는 경제적인 측면에서 유리할뿐만 아니라 간섭물질로부터의 영향이 적어 바이오센서에 적합한 전극으로 응용될 수 있으리라고 생각되어진다.As described above and demonstrated the present invention in detail as described above, the Pt metal ink electrode using screen printing was shown to have good glucose analysis performance and stability when a certain amount of carbon (C) ink is contained. This is not only economically advantageous, but it is thought that it may be applied as an electrode suitable for biosensors due to less influence from interference materials.

Claims (3)

스크린 프린팅 방법으로 Pt 금속 잉크에 탄소(C) 잉크를 섞어 만든 글루코스 분석용 전극의 혼합 비율Mixing ratio of electrode for glucose analysis made by mixing carbon (C) ink with Pt metal ink by screen printing method 제 1항에 있어서 제작된 전극의 최적 curing 시간The optimal curing time of the electrode prepared according to claim 1 글루코스 센서에 응용Application to glucose sensor
KR1020010072488A 2001-10-26 2001-11-20 Glucose Electrode Containing Pt metal and its efficiency KR20010110272A (en)

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KR1020010072488A KR20010110272A (en) 2001-11-20 2001-11-20 Glucose Electrode Containing Pt metal and its efficiency
KR10-2002-0034937A KR100453483B1 (en) 2001-10-26 2002-06-21 Patch for glucose extraction apparatus and manufacturing process for the same
KR2020020028684U KR200297594Y1 (en) 2001-10-26 2002-09-25 Patch for glucose extraction apparatus

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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004029611A1 (en) * 2002-09-24 2004-04-08 Hee-Chan Kim Mesoporous platinum electrode and method for detecting biochemical substrate using the mesoporous platinum electrode
EP1540351A1 (en) * 2002-08-30 2005-06-15 Biochec Co., Ltd. Glucose extraction patch and its manufacturing process
KR20190025354A (en) 2017-09-01 2019-03-11 동우 화인켐 주식회사 Glucose sensor and manufacturing method thereof
KR20190031944A (en) 2017-09-19 2019-03-27 동우 화인켐 주식회사 Glucose sensor
KR20190101203A (en) 2018-02-22 2019-08-30 동우 화인켐 주식회사 Glucose sensor
KR20190125620A (en) 2018-04-30 2019-11-07 동우 화인켐 주식회사 Glucose sensor
KR20200097627A (en) 2019-02-08 2020-08-19 동우 화인켐 주식회사 Bio sensor and method of manufacturing the same
KR20210062982A (en) 2019-11-22 2021-06-01 동우 화인켐 주식회사 Bio sensor
KR20210070720A (en) 2019-12-05 2021-06-15 동우 화인켐 주식회사 Bio sensor
CN114965630A (en) * 2022-06-06 2022-08-30 晨光生物科技集团股份有限公司 Silk-screen printing electrode for detecting antioxidant activity and preparation and detection methods thereof

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1540351A1 (en) * 2002-08-30 2005-06-15 Biochec Co., Ltd. Glucose extraction patch and its manufacturing process
EP1540351A4 (en) * 2002-08-30 2007-06-06 Biochec Co Ltd Glucose extraction patch and its manufacturing process
WO2004029611A1 (en) * 2002-09-24 2004-04-08 Hee-Chan Kim Mesoporous platinum electrode and method for detecting biochemical substrate using the mesoporous platinum electrode
US7892415B2 (en) 2002-09-24 2011-02-22 Cubiq, Inc Mesoporous platinum electrode and method for detecting biochemical substrate using the mesoporous platinum electrode
KR20190025354A (en) 2017-09-01 2019-03-11 동우 화인켐 주식회사 Glucose sensor and manufacturing method thereof
KR20190031944A (en) 2017-09-19 2019-03-27 동우 화인켐 주식회사 Glucose sensor
KR20190101203A (en) 2018-02-22 2019-08-30 동우 화인켐 주식회사 Glucose sensor
KR20190125620A (en) 2018-04-30 2019-11-07 동우 화인켐 주식회사 Glucose sensor
KR20200097627A (en) 2019-02-08 2020-08-19 동우 화인켐 주식회사 Bio sensor and method of manufacturing the same
KR20210062982A (en) 2019-11-22 2021-06-01 동우 화인켐 주식회사 Bio sensor
KR20210070720A (en) 2019-12-05 2021-06-15 동우 화인켐 주식회사 Bio sensor
CN114965630A (en) * 2022-06-06 2022-08-30 晨光生物科技集团股份有限公司 Silk-screen printing electrode for detecting antioxidant activity and preparation and detection methods thereof

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