KR20010093858A - New ethyl arizidine derivatives and their preparation methods - Google Patents

Abstract

PURPOSE: The ethyl aziridine derivatives and a producing method thereof are provided, thereby producing the intermediates used for synthesizing HIV protease. CONSTITUTION: The ethyl aziridine derivatives are represented by formula(II), in which R3 is selected from H, alkyl, aryl, alalkyl and amino protecting group, wherein the amino protecting group is C5 to C7 single membered hetero ring or C7 to C11 two membered hetero ring; R1 is silyl with the same or different alkyl substituents or alcohol protecting group, wherein the alcohol protecting group is one of alkoxycarbonyl, aryloxycarbonyl, methoxymethyl, tetrahydrofuran, methylethoxymethyl or ethoxyvinylethyl; Z is F, Cl, Br, I or -OSOnR5 wherein n is 0, 1, 2 and R5 is alkyl or aryl; and R7 is phenyl, phenyl having hydroxy, halogen atom, alkyl or alkoxy substituents at para site, phenylthio, pyridyl, piperidyl, cyclohexyl, alkyl or alkenyl having 4 or less of carbon number, or alkenyl with phenyl substituents.

Description

New ethyl arizidine derivatives and their preparation methods

The present invention relates to a novel ethyl aziridine derivative and a method for preparing the same, in particular a novel aziridine compound useful as an important intermediate for the synthesis of oligopeptide-like compounds including human immunodeficiency virus (HIV) protease inhibitor compounds and the preparation thereof It is about a method.

HIV is a retrovirus with genetic information in the form of RNA. Drugs for these viruses include reverse transcriptase inhibitors and HIV protease inhibitors, but most of them only prevent infection of cells, but do not prevent virus replication in already infected cells. This can be seen as strong. In addition, the rapid emergence of viruses that have developed resistance to these compounds is urgently needed to develop new therapeutic drugs.

The HIV protease inhibitor compounds developed so far include Roche's saquinavier (European Patent 432695 A (1991)), Glaxo-Welcome's Amprenavir (US Patent 941982 (1992)), Merck's Indinavir [US Patent 789508 (1991), Abbott's retronavir (US Pat. No. 998114 (1992)) and Agron's Nelpinavir (US Pat. No. 5,484,926 (1996)). These compounds are mainly used for the treatment or prevention of acquired immunodeficiency syndrome (AIDS) caused by HIV infection.

These HIV protease inhibitory compounds are those belonging to a class of inhibitors called hydroxyethylamines. Among them, saquinavier, parinavir, and amprenavier, except Nelpinavier, are characterized by having a benzyl group at the carbon position 2 in the skeleton of hydroxyethylamine (HEA).

As an intermediate for the synthesis of HIV protease inhibitor compounds thus far is (2 R) - [1 ' (S) - azido-2-phenylethyl] oxirane (J. Med Chem 1993, 36, 292-294.. ) And 3 ( S ) -amino-1,2 ( S ) -epoxy-4-phenylbutane. However, these intermediates always have a benzyl group in the skeleton of hydroxyethylamine (HEA) because phenylalanine is the starting material. On the other hand, because nepinavier has a phenylthiomethyl group instead of a benzyl group in the hydroxyethylamine skeleton, other intermediates such as 3 ( S ) -amino-1,2 ( S ) -epoxy-4-phenylthiobutane need.

Thus, the development of existing or new HIV protease inhibitor compounds requires new intermediates capable of introducing phenylthiomethyl and other substituents as well as benzyl groups.

Accordingly, it is an object of the present invention to provide novel ethyl aziridine derivatives which can introduce various substituents.

It is also an object of the present invention to provide important intermediates for the synthesis of HIV proteases.

The present invention relates to a novel ethyl aziridine derivative represented by the following general formula (I) and a method for producing the same. The novel ethyl aziridine derivatives of the present invention synthesize derivatives of ethyl aziridine wherein the amino and hydroxy groups are protected from tartaric acid and the ends are substituted with halogen and alkoxy. These aziridine derivatives can be usefully used as key intermediates for the preparation of HIV protease inhibitory compounds.

Wherein R ₁ and R ₂ are each selected from H, alkyl, allyl, alalkyl and hetero atoms, and the hetero atoms are linked to alkyl, allyl or alalkyl. R ₃ is selected from H, alkyl, allyl, alalkyl and amino protecting groups, wherein the nitrogen atom of the amino protecting group consists of 5-7 monocyclic hetero rings or 7-11 two ring hetero rings. X and Y are each selected from H, C ₁ -C ₄ lower alkyl, alalkyl, allyl or hetero atoms, and the hetero atoms are linked to alkyl, allyl or alalkyl. R ₄ is selected from silyl and alcohol protecting groups having the same or different alkyl substituents, and the alcohol protecting group is alkoxycarbonyl, aryloxycarbonyl, methoxymethyl, tetrahydrofuran, methylethoxymethyl, or ethoxyvinylethyl . Z is selected from F, Cl, Br, I, -OR ₅ . R ₅ is SOnR ₆ , defined as n = 0,1,2, and R ₆ is selected from alkyl or allyl groups.

The synthesis | combining method of the ethyl aziridine derivative of following general stone (1 ') whose R <_1> and R <_2> , X and Y is hydrogen is as follows.

When using D-tartaric acid as starting material

(A) D-tartaric acid is reacted with SOCl ₂ in methanol to form D-methyl tartarate.

(B) The reaction of D-methyl tartrate with 2,2-dimethoxypropene and para-toluene sulfonic acid in dichloromethane yields a 4,5-dimethoxycarbonyl acetonide compound.

(C) 2,3- 0 -isopropylidene-D-thritol is obtained by reducing 4,5-dimethoxycarbonyl acetonide from methanol to sodium borhydride.

(D) 2,3-0-isopropylidene reacted in acetonitrile with the -D- sseureyi tall tree and ethylamine, lithium halides and methane sulfonyl halide butane -2 (S) to the 1,4-dihalo, Prepare 3 ( S ) -diol.

(E) Dihalobutane-2 ( S ), 3 ( S ) -diol is added to thionyl chloride in carbon tetrachloride and oxidized to rudenium chloride and sodium periodate, or dihalobutane-2 ( S ), 3 ( S ) -diol is reacted with imidazole and sulfuryl chloride in dichloromethane to prepare 1,4-dihalobutane-2 ( S ), 3 ( S ) -diol sulfate.

(F) 1,4-dihalobutane-2 ( S ), 3 ( S ) -diol sulfate and phthalimide potassium salt were reacted in DMF to produce N- [1,4-dihalo-2 ( S ) -hydride. Roxy-3 ( R ) -butyl] phthalimide is prepared.

N- [1,4-dihalo-2 ( S ) -hydroxy-3 ( R ) -butyl] phthalimide in isopropanol with 80% hydrazine monohydrate to remove the phthalimide protecting group The resulting amine was reacted with acid anhydride and triethylamine in tetrahydrofuran (THF) to give 2 ( R )-(alkyloxycarbonyl) amino-1,4-dihalo-3 ( S ) -hydroxybutane. Manufacture.

(A) 2 ( R )-(alkyloxycarbonyl) amino-1,4-dichloro-3 ( S ) -hydroxybutane is reacted with a hydroxy protecting group-providing compound such as silane chloride or carbonyl chloride to give 2 ( S To prepare) -silyl (or carbonyl) oxy-3 ( R ) -alkyloxycarbonylamino-1,4-dihalobutane.

(I) N -alkyloxycarbonyl by reaction of 2 ( S ) -silyl (or carboyl) oxy-3 ( R ) -alkyloxycarbonylamino-1,4-dihalobutane with sodium hydride in THF Prepare -2 ( R )-[1 ( S ) -silyloxy-2-haloethyl] aziridine.

The preparation method is shown below as chemical reaction formula (1-a).

[Scheme 1-a]

When L-tartaric acid is used as a starting material, a compound of the following general formula (I-b) can be prepared by the same synthesis method as described above.

Two isomers of formula (I-a) and formula (I-b) are represented by the following formula (I ').

The present invention also provides novel compounds of the general formula (II).

Wherein R ₃ is selected from H, alkyl, aryl, alalkyl and amino protecting groups, the amino protecting group consisting of 5-7 monocyclic hetero rings or 7-11 bicyclic hetero rings, and R ₄ is Z is selected from silyl and alcohol protecting groups having the same or different alkyl substituents, and the protecting group is an alkoxycarbonyl, aryloxycarbonyl, methoxymethyl, tetrahydrofuran, methylethoxymethyl, or ethoxyvinylethyl, and Z is F, Cl, Br, I, or n = 0,1,2 and R ₅ is an alkyl or aryl -OSOnR _5, R ₇ is phenyl, phenyl having hydroxy, halogen atom, alkyl or alkoxy substituent in the para position , Phenylthio, pyridyl, piperidyl, cyclohexyl, alkyl or alkenyl having 4 or less carbon atoms, alkenyl having a phenyl substituent.

The compound of formula (II) is prepared by the following reaction from the ethyl aridine derivative of formula (I ').

[Chemical Scheme 2]

In the chemical scheme, R ₃ , R ₄ , Z, R ₇ are as described above and M is selected from alkali metal or alkaline earth metal halides.

The present invention also provides novel compounds of the general formula (III).

Wherein R ₃ is selected from H, alkyl, aryl, alalkyl and amino protecting groups, wherein the amino protecting group is composed of 5-7 monocyclic hetero rings or 7-11 bicyclic hetero rings, and R ₇ is Phenyl, phenyl, phenylthio, pyridyl, piperidyl, cyclohexyl, alkyl or alkenyl having up to 4 carbon atoms, alkenyl having phenyl substituents, having a hydroxy, halogen atom, alkyl or alkoxy substituent in the para position .

The compound of formula (III) is prepared by reacting the compound of formula (II) with tetrabutylammonium fluoride (TBAF).

[Chemical Scheme 3]

In addition, the amprenavier and nlpinavir are the following chemical reactions of the following formulas (II) and (III) wherein R ₃ is butyloxycarbonyl and R ₄ is t-butyldimethylsilyl. Prepared by Chemical Scheme 5, respectively. Of the compounds of formulas (II) and (III), R ₇ is a phenyl group, and becomes a raw material for synthesizing amprenavier, and when R ₇ is a phenylthio group, it is a synthetic raw material of nelfinavir.

[Chemical Scheme 4]

[Chemical Scheme 5]

Hereinafter, the present invention will be described in more detail with reference to Examples.

Synthesis of Ethyl Aziridine Derivatives from D-tartaric Acid

Example 1.

Preparation of Dimethyl D-tartarate

19.1 g (127 mmol) of D-tartaric acid was dissolved in anhydrous methanol (60 mL), and 48.3 mL (665 mmol) of thionyl chloride was added at 0 ° C., followed by stirring for 1 hour. After refluxing the reaction solution for 3 hours, hydrogen gas and methanol were removed under reduced pressure. After extraction with ethyl acetate (40 mL * 8) and water (40 mL), the organic layer was dried over anhydrous magnesium sulfate, filtered and concentrated under reduced pressure to give 22.5 g of a pale yellow liquid, dimethyl D-tartarate.

Example 2.

Preparation of Dimethyl 2,3- 0 -isopropylidene-D-tartarate

22.5 g (126 mmol) of dimethyl D-tartarate was dissolved in anhydrous dichloromethane, 12.0 g (63.0 mmol) of p -toluenesulphonic acid and 101 mL (825 mmol) of 2,2-dimethoxypropene were added and refluxed for 4 hours. It was. The solvent was removed under reduced pressure and 40 ml of water was added thereto. Extracted with ethyl acetate 100 ㎖ by drying over anhydrous magnesium sulfate, filtered and concentrated under reduced pressure to a liquid in the red-dimethyl-2,3-0-isopropylidene -D- give the tartrate 24.1 g (124 mmol).

Example 3.

Preparation of 2,3- 0 -isopropylidene-D-thritol

24 g (124 mmol) of dimethyl 2,3- 0 -isopropylidene-D-tartarate was dissolved in 400 ml of methanol, and 23.4 g (618 mmol) of sodium borohydride was added at 0 ° C. and stirred for 4 hours. At this time, the reaction temperature rises to room temperature. The solvent was blown out under reduced pressure, concentrated, and then 400 ml of distilled water was added and extracted with ethyl acetate (400 ml * 4). The solution was dried over anhydrous magnesium sulfate, filtered and sent away the solvent under reduced pressure, the pale yellow liquid 2,3-0-isopropylidene -D- sseureyi tolyl to give the 17.2g (106 mmol).

Example 4.

Preparation of 1,4-dichlorobutane-2 ( S ), 3 ( S ) -diol

2,3 0-isopropylidene -D- sseureyi toll 17.2 g (106 mmol) of acetonitrile was dissolved the reel 300㎖ 34.0 ㎖ (244 mmol) of triethylamine at 0 ℃, lithium chloride 40.0 ㎖ (944 mmol) and 17.3 ml (224 mmol) of methanesulfonyl chloride was added and stirred for 30 minutes. The reaction solution was refluxed overnight and then concentrated under reduced pressure. The concentrate was neutralized with sodium bicarbonate and extracted with ethyl acetate (500 mL * 2). The solution was dried over anhydrous magnesium sulfate, filtered and the solvent was blown off under reduced pressure to give a dark brown liquid. Treatment with distilled water (200 mL) and n -hexane (200 mL) removed the non-polar dark brown impurities. The organic layer was extracted with distilled water (200 mL) and ethyl acetate (200 mL * 4), dried over anhydrous magnesium sulfate, filtered and the solvent was removed under reduced pressure to give a white solid of 1,4-dichlorobutane-2 ( S ), 8.35 g (52.5 mmol) of 3 ( S ) -diol were obtained.

Example 5.

Preparation of 1,4-dichlorobutane-2 ( S ), 3 ( S ) -diol sulfate

Method A

159 mg (1.0 mmol) of 1,4-dichlorobutane-2 ( S ), 3 ( S ) -diol was dissolved in 1 mL of carbon tetrachloride, and 88 μl (1.2 mmol) of thionyl chloride was added and refluxed for 30 minutes. The solvent was removed under reduced pressure, and 3 ml of cold carbon tetrachloride and 3 ml of acetonitrile were added, and 4.5 ml of cold distilled water was added while cooling in an ice-bath. 1.15 mg (0.01 mmol) of ruthenium chloride hydrate and 0.428 g (2.0 mmol) of sodium periodate were added all at once and stirred vigorously at 0 ° C. After stirring for 1 hour, 6 ml of ethyl ether was added thereto, and the aqueous layer was extracted with ethyl ether (2.5 ml * 3). The organic layer was washed with brine (3 mL), dried over anhydrous magnesium sulfate, filtered and the solvent was removed under reduced pressure to obtain 206.4 mg of a white solid. Ethyl acetate / n -hexane = 1/3 was used as the eluent to separate silica gel column chromatography to obtain 1,4-dichlorobutane-2 ( S ), 3 ( S ) -diol sulfate as a white solid.

Method B.

0.10 mL (1.258) of sulfuryl chloride in 2.1 mL of dichloromethane solution containing 100.3 mg (0.631 mmol) of 1,4-dichlorobutane-2 ( S ), 3 ( S ) -diol and imidazole (85.8 mg (1.26 mmol)) mmol) was dropped dropwise at 27 ° C. A precipitate formed as the reaction proceeded, and the reaction mixture was continued to stir at 27 ° C. When the reaction was completed, 2.5 ml of dichloromethane was added, followed by 0.75 sulfuric acid aqueous solution 0.75. ML was added and the organic layer was separated, and the separated organic layer was washed with 0.75 mL of saturated aqueous sodium bicarbonate solution and saturated brine, dried over anhydrous magnesium sulfate, and distilled under reduced pressure to obtain 98.1 mg (70.3%) of the product.

Example 6.

Preparation of N- [1,4-dichloro-2 ( S ) -hydroxy-3 ( R ) -butyl] phthalimide

57.2 mg (0.26 nnol) of 1,4-dichlorobutane-2 ( S ), 3 ( S ) -diol sulfate and 51.4 mg (0.27 mmol) of phthalimide potassium salt were dissolved in 1.3 ml of anhydrous DMF and charged at room temperature under nitrogen gas filling. Stir for 1 hour. The solvent was blown off under reduced pressure and dissolved in 3 mL of anhydrous THF. Pour into 13 μl sulfuric acid and 5 μl of distilled water and stirred for 30 minutes. Excess sodium bicarbonate (˜52 mg) was added and stirred for 30 minutes. Filter through celite and silica gel beds and blow off the solvent under reduced pressure to yield 77.8 mg of a colorless liquid N- [1,4-dichloro-2 ( S ) -hydroxy-3 ( R ) -butyl] phthalimide. Got it.

Example 7.

Preparation of 2 ( R )-( t -butyloxycarbonyl) amino-1,4-dichloro-3 ( S ) -hydroxybutane

48.6 mg (0.17 mmol) of N- [1,4-dichloro-2 ( S ) -hydroxy-3 ( R ) -butyl] phthalimide was dissolved in isopropanol (1 mL) and 80% hydrazine under 0 ° C. nitrogen stream. 11.3 μl (0.19 mmol) of the monohydrate was added thereto, followed by stirring for 16 hours. The solvent was blown off under reduced pressure to give a solid. The solid was dissolved in methanol (1 mL), 35% HCl solution (21.0 μl, 0.24 mmol) was added and stirred at room temperature for 16 hours. The solvent was blown off under reduced pressure, and the residue was dissolved in THF (0.5 mL) and distilled water (0.5 mL), and 46.5 mg (0.213 mmol) of trichloroamine and 49.5 μl (0.355 mmol) of triethylamine were stirred at 0 ° C. for 16 hours. . The reaction solution was diluted with 1 mL of distilled water and extracted with ethyl acetate (2 mL * 3). The organic layer was washed with 1 ml of brine, dried over anhydrous magnesium sulfate, filtered and the solvent was blown off under reduced pressure. Ethyl acetate: n -hexane = 1: 4 eluent, isolated by silica gel column chromatography, white solid 2 ( R )-( t -butyloxycarbonyl) amino-1,4-dichloro-3 ( S ) Gave 32.7 mg of hydroxybutane

Yield: 75%

Melting Point: 142∼145 ℃

Example 8.

Preparation of 2 ( S ) -t -butyldimethylsilyloxy-3 ( R )-( t -butyloxycarbonyl) amino-1,4-dichlorobutane

100 mg (0.389 mmol) of 2 ( R )-( t -butyloxycarbonyl) amino-1,4-dichloro-3 ( S ) -hydroxybutane were dissolved in 2.5 mL of anhydrous DMF and 79.4 mg of imidazole in a nitrogen stream. 1.67 mmol), 4.8 mg (0.039 mmol) of DMAP, and 176 mg (1.167 mmol) of TBSCl were added thereto, and the resultant was stirred at 50 ° C. for 12 hours. The reaction solution was diluted with 30 ml of ethyl acetate and washed sequentially with 5 ml of 0.1 N citric acid solution, 5 ml of saturated sodium bicarbonate solution, 5 ml of distilled water and 5 ml of brine. The organic layer was dried over anhydrous magnesium sulfate, filtered and the solvent was blown off under reduced pressure. Ethyl acetate: n -hexane = 1: 10 eluent separated by silica gel column chromatography to give a colorless solid 2 ( S ) -t-butyldimethylsilyloxy-3 ( R )-( t -butyloxycarba Bonyl) amino-1,4-dichlorobutane 144.6 mg was obtained

Yield: 99%

Example 9

Preparation of 2 ( S ) -t -butyldimethylsilyloxy-2 ( R )-[1 ( S ) -t -butyldimethylsilyloxy-2-chloroethyl] aziridine

36.0 mg (0.107 mmol) of 2 ( S ) -t -butyldimethylsilyloxy-3 ( R )-( t -butyloxycarbonyl) amino-1,4-dichlorobutane was dissolved in 10 ml of THF, and 0 ° C, Sodium hydride was slowly added to the nitrogen stream and stirred for 5 hours. 40 ml of saturated ammonium aqueous solution and 20 g of ice were added to the reaction mixture to stop the reaction, and the mixture was extracted with ethyl acetate (60 ml * 4). The organic layer was washed with 50 ml of brine, dried over anhydrous magnesium sulfate, filtered and the solvent was blown off under reduced pressure. Ethyl Acetate: 2 ( S ) -t -butyldimethylsilyloxy-2 ( R )-[1 ( S )-, a colorless liquid, separated by silica gel column chromatography with n -hexane = 1: 10 eluent. 339.5 mg of t -butyldimethylsilyloxy-2-chloroethyl] aziridine were obtained

Yield: 99%

Example 10.

Preparation of 2 ( S ) -t -butyldimethylsilyloxy-3 ( S )-( t -butyloxycarbonyl) amino-1-chloro-4-phenylbutane

Dissolve 36.0 mg (0.107 mmol) of 2 ( S ) -t -butyldimethylsilyloxy-2 ( R )-[1 ( S ) -t -butyldimethylsilyloxy-2-chloroethyl] aziridine in toluene 11.0 mg (0.054 mmol) of copper bromide dimethyl sulfide complex was added to the air stream. The reaction temperature was lowered to −78 ° C., 0.54 mL (1.072 mmol, 2 M solution in THF) of phenylmagnesium chloride was added, and stirred at −20 ° C. overnight. 4 ml of saturated ammonium chloride was added to the reaction mixture, followed by extraction with ethyl acetate (5 ml * 3). The organic layer was washed with 50 ml of brine and dried over anhydrous magnesium sulfate. The solid was filtered off and the solvent was blown off under reduced pressure. Ethyl acetate: n -hexane = 3: 10 Eluent separated by silica gel column chromatography to give a colorless liquid 2 ( S ) -t -butyldimethylsilyloxy-3 ( S )-( t -butyloxycarba 33.5 mg of carbonyl) amino-1-chloro-4-phenylbutane was obtained

Yield: 75%

Example 11.

Preparation of 3 ( S )-( t -butyloxycarbonyl) amino-1,2 ( S ) -epoxy-4-phenylbutane

30 mg (0.073 mmol) of 2 ( S ) -t -butyldimethylsilyloxy-3 ( S )-( t -butyloxycarbonyl) amino-1-chloro-4-phenylbutane was dissolved in 0.5 ml of THF and 0 ° C. In TBAF 0.11 mL (0.109 mmol, 1.0 M solution in THF) was added and stirred at room temperature for 40 minutes. 0.5 ml of saturated ammonium chloride was added to the reaction mixture, diluted with 2 ml of distilled water, and extracted with ethyl acetate (3 ml * 3). The organic layer was washed with 3 ml of brine and dried over anhydrous magnesium sulfate. The solid was filtered off and the solvent was blown off under reduced pressure. The residue was dissolved in 0.5 mL of methanol and treated with KOH at 0 ° C. After stirring for 2 hours at room temperature, the solvent was blown off under reduced pressure. The residue was diluted with 2 mL of distilled water and extracted with ethyl acetate (3 mL * 3). The organic layer was washed with 3 ml of brine and dried over anhydrous magnesium sulfate. The solid was filtered off and the solvent was blown off under reduced pressure. Ethyl Acetate: 3 ( S )-( t -butyloxycarbonyl) amino-1,2 ( S ) -epoxy- as a white solid, separated by silica gel column chromatography with n -hexane = 1: 10 eluent. 14.4 mg of 4-phenylbutane were obtained.

Yield: 75%

Synthesis of Amprenavir from Ethyl Aziridine Derivatives

Example 12

Preparation of 2 ( S ) -hydroxy-3 ( S )-( t -butyloxycarbonyl) amino-1-isobutylamino-4-phenylbutane

200 mg (0.76 mmol) of 3 ( S )-( t -butyloxycarbonyl) amino-1,2 ( S ) -epoxy-4-phenylbutane are dissolved in 2 ml of anhydrous isopropanol and 0.38 ml (3.8 mmol) of isobutylamine. The mixture was stirred for 5 hours at 50 ℃. The solvent was blown off and dried under reduced pressure to obtain 230 mg of 2 ( S ) -hydroxy-3 ( S )-( t -butyloxycarbonyl) amino-1-isobutylamino-4-phenylbutane.

Yield: 90%

Example 13.

Preparation of 2 ( S ) -hydroxy-3 ( S )-( t -butyloxycarbonyl) amino-1- (4-nitrobenzenesulfonyl) -1-isobutylamino-4-phenylbutane

64 mg (0.19 mmol) of 2 ( S ) -hydroxy-3 ( S )-( t -butyloxycarbonyl) amino-1-isobutylamino-4-phenylbutane was dissolved in 1 ml of anhydrous dichloromethane and 30 μl (0.23 mmol) of ethylamine and 55 mg (0.25 mmol) of 4-nitrobenzenesulfonyl chloride were added thereto, stirred for 30 minutes, and stirred at room temperature for 12 hours. The reaction solution was poured into 2 ml of saturated sodium bicarbonate and extracted with 10 ml of diethyl ether. The organic layer was dried over anhydrous magnesium sulfate, the solid was filtered off, and the solvent was blown off under reduced pressure. Separation by silica gel column chromatography 2 ( S ) -hydroxy-3 ( S )-( t -butyloxycarbonyl) amino-1- (4-nitrobenzenesulfonyl) -1-isobutylamino-4- 82.0 mg of phenylbutane was obtained

Yield: 88%

Example 14.

2 ( S ) -hydroxy-3 ( S )-[(S) -tetrahydrofuran-3-yloxycarbonyl] amino-1- (4-nitrobenzenesulfonyl) -1-isobutylamino-4- Preparation of Phenylbutane

2 ( S ) -hydroxy-3 ( S )-( t -butyloxycarbonyl) amino-1- (4-nitrobenzenesulfonyl) -1-isobutylamino-4-phenylbutane 93 mg (0.19 mmol) Was dissolved in 1 ml of anhydrous dichloromethane and anhydrous hydrochloric acid gas was flowed for 10 minutes. The chlorine gas was blown off by bubbling and stirred for 1 hour. The solvent was blown off under reduced pressure and the residue was taken up in 1 ml of dichloromethane. 30 µl (0.23 mmol) of triethylamine and 44 mg (0.19 mmol) of N -succinimidyl ( S )-(+)-3-hydroxytetrahydrofurancarbonate were added to the reaction solution and stirred at room temperature for 4 hours. The reaction solution was poured into 2 ml of brine and extracted with 10 ml of ethyl acetate. The organic layer was dried over anhydrous magnesium sulfate, the solid was filtered off, and the solvent was blown off under reduced pressure. The residue was recrystallized from ethyl acetate to give 2 ( S ) -hydroxy-3 ( S )-[(S) -tetrahydrofuran-3-yloxycarbonyl] amino-1- (4-nitrobenzenesulfonyl) -1 86 mg of isobutylamino-4-phenylbutane were obtained.

Yield: 85%

Example 15.

2 ( S ) -hydroxy-3 ( S )-[( S ) -tetrahydrofuran-3-yloxycarbonyl] amino-1- (4-aminobenzenesulfonyl) -1-isobutylamino-4- Preparation of Phenylbutane

2 ( S ) -hydroxy-3 ( S )-[( S ) -tetrahydrofuran-3-yloxycarbonyl] amino-1- (4-nitrobenzenesulfonyl) -1-isobutylamino-4- 36 mg (0.067 mmol) of phenylbutane and 77 mg (0.34 mmol) of SnCl 2 .H 2 O were refluxed at 70 ° C. in 1 ml of ethyl acetate for 1 hour. The reaction solution was cooled to room temperature, poured into 3 ml of saturated sodium bicarbonate solution, and extracted with 10 ml of ethyl acetate. The organic layer was dried over anhydrous magnesium sulfate and the solvent was blown off under reduced pressure to give 2 ( S ) -hydroxy-3 ( S )-[( S ) -tetrahydrofuran-3-yloxycarbonyl] amino-1- (4-amino 30 mg of benzenesulfonyl) -1-isobutylamino-4-phenylbutane were obtained.

Yield: 90%

Synthesis of Ethyl Aziridine Derivatives from L-tartaric Acid

Example 16.

Preparation of Dimethyl L-tartarate

19.1 g (127 mmol) of L-tartaric acid was dissolved in anhydrous methanol (60 mL), and 48.3 mL (665 mmol) of thionyl chloride was added at 0 ° C., followed by stirring for 1 hour. After refluxing the reaction solution for 3 hours, hydrogen gas and methanol were removed under reduced pressure. After extracting with ethyl acetate (40 mL * 8) and water (40 mL), the organic layer was dried over anhydrous magnesium sulfate, filtered and concentrated under reduced pressure to give 22.5 g of dimethyl L-tartarate as a pale yellow liquid.

Example 17.

Preparation of Dimethyl 2,3- 0 -isopropylidene-L-tartarate

22.5 g (126 mmol) of dimethyl L-tartarate was dissolved in anhydrous dichloromethane, 12.0 g (63.0 mmol) of p -toluenesulphonic acid and 101 mL (825 mmol) of 2,2-dimethoxypropene were added and refluxed for 4 hours. It was. The solvent was removed under reduced pressure and 40 ml of water was added thereto. Extracted with ethyl acetate 100 ㎖ by drying over anhydrous magnesium sulfate, filtered and concentrated under reduced pressure to a liquid in the red-dimethyl-2,3-0-isopropylidene -L- give the tartrate 24.1 g (124 mmol).

Example 18.

Preparation of 2,3- 0 -isopropylidene-L-thritol

2,3-dimethyl-0-isopropylidene -L- tartarate 24 g (124 mmol) of sodium borohydride were dissolved 23.4 g (618 mmol) at 0 dissolved in 400 ℃ ㎖ methanol was stirred for 4 hours. At this time, the reaction temperature rises to room temperature. The solvent was blown off under reduced pressure, concentrated, and then 400 ml of distilled water was added and extracted with ethyl acetate (400 ml * 4). The solution was dried over anhydrous magnesium sulfate, filtered and sent away the solvent under reduced pressure, the pale yellow liquid 2,3-0-isopropylidene -L- sseureyi tolyl to give the 17.2g (106 mmol).

Example 19.

Preparation of 1,4-dichlorobutane-2 ( R ), 3 ( R ) -diol

17.2 g (106 mmol) of 2,3- O -isopropylidene-L-thritol is dissolved in 300 ml of acetonitrile and 34.0 ml (244 mmol) of triethylamine, 40.0 ml (944 mmol) of lithium chloride and methane at 0 ° C. 17.3 ml (224 mmol) of sulfonyl chloride was added and stirred for 30 minutes. The reaction solution was refluxed overnight and then concentrated under reduced pressure. The concentrate was neutralized with sodium bicarbonate and extracted with ethyl acetate (500 mL * 2). The solution was dried over anhydrous magnesium sulfate, filtered and the solvent was blown off under reduced pressure to give a dark brown liquid. Treatment with distilled water (200 mL) and n -hexane (200 mL) removed the non-polar dark brown impurities. The organic layer was extracted with distilled water (200 mL) and ethyl acetate (200 mL * 4), dried over anhydrous magnesium sulfate, filtered and the solvent was removed under reduced pressure to give a white solid of 1,4-dichlorobutane-2 ( R ), 8.35 g (52.5 mmol) of 3 ( R ) -diol were obtained.

Example 20

Preparation of 1,4-dichlorobutane-2 ( R ), 3 ( R ) -diol sulfate

Method A.

159 mg (1.0 mmol) of 1,4-dichlorobutane-2 ( R ), 3 ( R ) -diol was dissolved in 1 ml of carbon tetrachloride, and 88 μl (1.2 mmol) of thionyl chloride was added and refluxed for 30 minutes. After blowing, 3 ml of cold carbon tetrachloride and 3 ml of acetonitrile were added, and 4.5 ml of cold distilled water was added while cooling in an ice-bath. 1.15 mg (0.01 mmol) of ruthenium chloride hydrate and 0.428 g (2.0 mmol) of sodium periodate were added all at once and stirred vigorously at 0 ° C. After stirring for 1 hour, 6 ml of ethyl ether was added thereto, and the aqueous layer was extracted with ethyl ether (2.5 ml * 3). The organic layer was washed with brine (3 mL), dried over anhydrous magnesium sulfate, filtered and the solvent was removed under reduced pressure to obtain 206.4 mg of a white solid. Ethyl acetate / n -hexane = 1/3 was used as the eluent to separate silica gel column chromatography to obtain 1,4-dichlorobutane-2 ( R ), 3 ( R ) -diol sulfate as a white solid.

Method B.

0.10 mL (1.258) of sulfuryl chloride in 2.1 mL of dichloromethane solution containing 100.3 mg (0.631 mmol) of 1,4-dichlorobutane-2 ( R ), 3 ( R ) -diol and imidazole (85.8 mg (1.26 mmol)) mmol) was dropped dropwise at 27 ° C. A precipitate formed as the reaction proceeded, and the reaction mixture was continued to stir at 27 ° C. When the reaction was completed, 2.5 ml of dichloromethane was added, followed by 0.75 sulfuric acid aqueous solution 0.75. ML was added and the organic layer was separated, and the separated organic layer was washed with 0.75 mL of saturated aqueous sodium bicarbonate solution and saturated brine, dried over anhydrous magnesium sulfate, and distilled under reduced pressure to obtain 98.1 mg (70.3%) of the product.

Example 21.

Preparation of N- [1,4-dichloro-2 ( R ) -hydroxy-3 ( S ) -butyl] phthalimide

57.2 mg (0.26 nnol) of 1,4-dichlorobutane-2 ( R ), 3 ( R ) -diol sulfate and 51.4 mg (0.27 mmol) of phthalimide potassium salt were dissolved in 1.3 ml of anhydrous DMF and charged at room temperature under nitrogen gas filling. Stir for 1 hour. The solvent was blown off under reduced pressure and dissolved in 3 mL of anhydrous THF. Pour into 13 μl sulfuric acid and 5 μl of distilled water and stirred for 30 minutes. Excess sodium bicarbonate (˜52 mg) was added and stirred for 30 minutes. Filter through celite and silica gel beds and blow off the solvent under reduced pressure to yield 77.8 mg of a colorless liquid N- [1,4-dichloro-2 ( R ) -hydroxy-3 ( S ) -butyl] phthalimide. Got it.

Example 22.

Preparation of 2 ( S )-( t -butyloxycarbonyl) amino-1,4-dichloro-3 ( R ) -hydroxybutane

48.6 mg (0.17 mmol) of N- [1,4-dichloro-2 ( S ) -hydroxy-3 ( S ) -butyl] phthalimide was dissolved in isopropanol (1 mL) and 80% hydrazine under 0 ° C. nitrogen stream. 11.3 μl (0.19 mmol) of the monohydrate was added thereto, followed by stirring for 16 hours. The solvent was blown off under reduced pressure to give a solid. The solid was dissolved in methanol (1 mL), 35% HCl solution (21.0 μl, 0.24 mmol) was added thereto, and the mixture was stirred at room temperature for 16 hours. The solvent was blown off under reduced pressure, and the residue was dissolved in THF (0.5 mL) and distilled water (0.5 mL), and 46.5 mg (0.213 mmol) of trichloroamine and 49.5 μl (0.355 mmol) of triethylamine were stirred at 0 ° C. for 16 hours. . The reaction solution was diluted with 1 mL of distilled water and extracted with ethyl acetate (2 mL * 3). The organic layer was washed with 1 ml of brine, dried over anhydrous magnesium sulfate, filtered and the solvent was blown off under reduced pressure. Ethyl acetate: n -hexane = 1: 4 eluent separated by silica gel column chromatography, white solid 2 ( S )-( t -butyloxycarbonyl) amino-1,4-dichloro-3 ( R 32.7 mg of hydroxybutane was obtained

Yield: 75%

Melting Point: 142 ~ 145 ℃

Example 23.

Preparation of 2 ( R ) -t -butyldimethylsilyloxy-3 ( S )-( t -butyloxycarbonyl) amino-1,4-dichlorobutane

100 mg (0.389 mmol) of 2 ( S )-( t -butyloxycarbonyl) amino-1,4-dichloro-3 ( R ) -hydroxybutane were dissolved in 2.5 ml of anhydrous DMF and 79.4 mg of imidazole in a nitrogen stream. 1.67 mmol), 4.8 mg (0.039 mmol) of DMAP, and 176 mg (1.167 mmol) of TBSCl were added thereto, and the resultant was stirred at 50 ° C. for 12 hours. The reaction solution was diluted with 30 ml of ethyl acetate and washed sequentially with 5 ml of 0.1 N citric acid solution, 5 ml of saturated sodium bicarbonate solution, 5 ml of distilled water, and 5 ml of brine. The organic layer was dried over anhydrous magnesium sulfate, filtered and the solvent was blown off under reduced pressure. Ethyl acetate: n -hexane = 1: 10 eluent separated by silica gel column chromatography to give a colorless solid 2 ( R ) -t-butyldimethylsilyloxy-3 ( S )-( t -butyloxycar Bonyl) amino-1,4-dichlorobutane 144.6 mg was obtained

Yield: 99%

Example 24.

2 ( R ) -t -butyldimethylsilyloxy-2 ( S )-[1 ( R ) -t -butyldimethylsilyloxy-2-chloroethyl]

Preparation of Aziridine

Dissolve 36.0 mg (0.107 mmol) of 2 ( R ) -t -butyldimethylsilyloxy-3 ( S )-( t -butyloxycarbonyl) amino-1,4-dichlorobutane in 10 ml of THF, and nitrogen at 0 ° C. Sodium hydride was slowly added to the stream and stirred for 5 hours. 40 ml of saturated ammonium aqueous solution and 20 g of ice were added to the reaction mixture to stop the reaction, and the mixture was extracted with ethyl acetate (60 ml * 4). The organic layer was washed with 50 ml of brine, dried over anhydrous magnesium sulfate, filtered and the solvent was blown off under reduced pressure. Ethyl acetate: n -hexane = 1: 10 Eluent separated by silica gel column chromatography to give a colorless liquid 2 ( R ) -t -butyldimethylsilyloxy-2 ( S )-[1 ( R )- 339.5 mg of t -butyldimethylsilyloxy-2-chloroethyl] aziridine were obtained.

Yield: 99%

Example 25.

Preparation of 2 ( R ) -t -butyldimethylsilyloxy-3 ( R )-( t -butyloxycarbonyl) amino-1-chloro-4-phenylbutane

36.0 mg (0.107 mmol) of 2 ( R ) -t -butyldimethylsilyloxy-2 ( S )-[1 ( R ) -t -butyldimethylsilyloxy-2-chloroethyl] aziridine was dissolved in toluene and nitrogen. 11.0 mg (0.054 mmol) of copper bromide dimethyl sulfide complex was added to the air stream. The reaction temperature was lowered to -78 ° C and 0.54 mL (1.072 mmol, 2 M solution in THF) of phenylmagnesium chloride was added and stirred at -20 ° C overnight. 4 ml of saturated ammonium chloride was added to the reaction mixture, followed by extraction with ethyl acetate (5 ml * 3). The organic layer was washed with 50 ml of brine and dried over anhydrous magnesium sulfate. The solid was filtered off and the solvent was blown off under reduced pressure. Ethyl acetate: n -hexane = 3: 10 Eluent separated by silica gel column chromatography to give a colorless liquid 2 ( R ) -t -butyldimethylsilyloxy-3 ( R )-( t -butyloxycarba 33.5 mg of carbonyl) amino-1-chloro-4-phenylbutane was obtained

Yield: 75%

Example 26.

Preparation of 3 ( R )-( t -butyloxycarbonyl) amino-1,2 ( R ) -epoxy-4-phenylbutane

30 mg (0.073 mmol) of 2 ( R ) -t -butyldimethylsilyloxy-3 ( R )-( t -butyloxycarbonyl) amino-1-chloro-4-phenylbutane was dissolved in 0.5 ml of THF and 0 ° C. In TBAF 0.11 mL (0.109 mmol, 1.0 M solution in THF) was added and stirred at room temperature for 40 minutes. 0.5 ml of saturated ammonium chloride was added to the reaction mixture, diluted with 2 ml of distilled water, and extracted with ethyl acetate (3 ml * 3). The organic layer was washed with 3 ml of brine and dried over anhydrous magnesium sulfate. The solid was filtered off and the solvent was blown off under reduced pressure. The residue was dissolved in 0.5 mL of methanol and treated with KOH at 0 ° C. After stirring for 2 hours at room temperature, the solvent was blown off under reduced pressure. The residue was diluted with 2 mL of distilled water and extracted with ethyl acetate (3 mL * 3). The organic layer was washed with 3 ml of brine and dried over anhydrous magnesium sulfate. The solid was filtered off and the solvent was blown off under reduced pressure. Ethyl acetate: n -hexane = 1: 10 eluent separated by silica gel column chromatography to give a white solid 3 ( R )-( t -butyloxycarbonyl) amino-1,2 ( R ) -epoxy- 14.4 mg of 4-phenylbutane were obtained

Yield: 75%

Newly synthesized N - t -butyloxycarbonyl-2 ( R )-[1 ( S ) -t -butyldimethylsilyloxy-2-chloroethyl] aziridine has been described as amprinavir in HIV protease inhibitory compounds and It can be used as an important intermediate for the synthesis of Nelfinavier. In other words, 3 ( S )-( t -butyloxycarbonyl) amino-1,2 ( S ) -epoxy-4-phenyl, which is a key intermediate for the synthesis of amprinavir, is introduced by introducing a phenyl group into a ring opening reaction. Butane may be prepared. In addition, 3 ( S )-( t -butyloxycarbonyl) amino-1,2 ( S ) -epoxy-4-phenylthiobutane can be produced by introducing a phenylthio group instead of a phenyl group. This compound is a key intermediate for the synthesis of nelfinavir. In addition, N - t -butyloxycarbonyl-2 ( S )-[1 ( R ) -t -butyldimethylsilyloxy-2-chloroethyl] aziridine, an isomer of the above compound, has a steric opposite configuration. Synthesis of HIV protease inhibitory compounds with oxyethylamine backbones is possible.

Claims (5)

Compound of the following general formula (II): Wherein R ₃ is selected from H, alkyl, aryl, alalkyl and amino protecting groups, wherein the amino protecting group is composed of 5-7 monocyclic hetero rings or 7-11 two-ring hetero rings, and R ₁ is A silyl group having an identical or different alkyl substituent and an alcohol protecting group, the alcohol protecting group is alkoxycarbonyl, aryloxycarbonyl, methoxymethyl, tetrahydrofuran, methylethoxymethyl, or ethoxyvinylethyl, and Z is F, Cl, Br, I, or n = 0,1,2 and R ₅ is an alkyl or aryl -OSOnR _5, R ₇ is phenyl, phenyl having hydroxy, halogen atom, alkyl or alkoxy substituent in the para position , Phenylthio, pyridyl, piperidyl, cyclohexyl, alkyl or alkenyl having 4 or less carbon atoms, alkenyl having a phenyl substituent. Compounds of the following general formula (I ') are those in which R ₇ is phenyl; And alkenyl having a phenyl substituent and reacting with R ₇ M, wherein M is an alkali metal or an alkaline earth metal halide. Wherein R ₃ is selected from H, alkyl, aryl, alalkyl and amino protecting groups, the amino protecting group consisting of 5-7 monocyclic hetero rings or 7-11 two-ring hetero rings, and R ₄ is A silyl group having an identical or different alkyl substituent and an alcohol protecting group, the alcohol protecting group is alkoxycarbonyl, aryloxycarbonyl, methoxymethyl, tetrahydrofuran, methylethoxymethyl, or ethoxyvinylethyl, and Z is F, Cl, Br, I, or n = 0,1,2 and R ₅ is an alkyl or aryl -OSOnR _5, R ₇ is phenyl, phenyl having hydroxy, halogen atom, alkyl or alkoxy substituent in the para position , Phenylthio, pyridyl, piperidyl, cyclohexyl, alkyl or alkenyl having 4 or less carbon atoms, alkenyl having a phenyl substituent. According to claim 2, wherein the ethyl aziridine derivative of the general formula (I ') (a) reacting 1,4-dihalobutanediol sulfate with phthalimide potassium salt to produce N- [1,4-dihalo-2-hydroxy-3-butyl] phthalimide; (b) The amine formed by removing the phthalimide protecting group of N- [1,4-dihalo-2-hydroxy-3-butyl] phthalimide was reacted with acid anhydride and triethylamine in THF. preparing-( t -alkyloxycarbonyl) amino-1,4-dihalo-3-hydroxybutane; (c) 2-silyl (or carbonyl) oxy-3- by reaction of 2- ( t -alkyloxycarbonyl) amino-1,4-dihalo-3-hydroxybutane with silane chloride or carbonyl chloride Preparing (alkyloxycarbonyl) amino-1,4-dihalobutane; (d) 2- t -silyl (or carbonyl) oxy-3- ( t -alkyloxycarbonyl) amino-1,4-dihalobutane to reduce N - t -alkyloxycarbonyl-2- [1 t -silyl (carbonyl) oxy-2-haloethyl] aziridine. Compound of the following general formula (III): Wherein R ₃ is selected from H, alkyl, aryl, alalkyl and amino protecting groups, wherein the amino protecting group is composed of 5-7 monocyclic hetero rings or 7-11 bicyclic hetero rings, and R ₇ is Phenyl, phenyl, phenylthio, pyridyl, piperidyl, cyclohexyl, alkyl or alkenyl having up to 4 carbon atoms, alkenyl having phenyl substituents, having a hydroxy, halogen atom, alkyl or alkoxy substituent in the para position . A process for producing a compound of formula (III), wherein the compound of formula (II) is reacted with TBAF. Wherein R ₃ is selected from H, alkyl, aryl, alalkyl and amino protecting groups, the amino protecting group consisting of 5-7 monocyclic hetero rings or 7-11 two-ring hetero rings, and R ₄ is A silyl group having an identical or different alkyl substituent and an alcohol protecting group, the alcohol protecting group is alkoxycarbonyl, aryloxycarbonyl, methoxymethyl, tetrahydrofuran, methylethoxymethyl, or ethoxyvinylethyl, and Z is F, Cl, Br, I, or n = 0,1,2 and R ₅ is an alkyl or aryl -OSOnR _5, R ₇ is phenyl, phenyl having hydroxy, halogen atom, alkyl or alkoxy substituent in the para position , Phenylthio, pyridyl, piperidyl, cyclohexyl, alkyl or alkenyl having 4 or less carbon atoms, alkenyl having a phenyl substituent.