KR20010089932A - Process for Preparing Chiroinositol Extract from Edible Materials - Google Patents

Abstract

PURPOSE: A process for preparing chiro-inositol from resources for food such as pulses, soybean, soybean germ, defatted soybean, pine needle, pine sprout, pine sawdust or the like is provided. Whereby, the product can be used as an agent for treating a diabetic and material for drink, confectionery, bread, ice and gums. CONSTITUTION: The subjected chiro-inositol is prepared by the process consisting of: obtaining supernatant liquid by crushing resources for food such as pulses, soybean, soybean germ, defatted soybean, bean chaff, soybean oligosaccharide, bean sprout, pine needle, pine sprout and pine sawdust, extracting at 65 to 100deg.C for 10min to 2hr and filtering; obtaining acid-hydrolyzed liquid by removing protein and sugar from the supernatant and adding an acid; neutralizing the acid-hydrolyzed liquid; and obtaining a chiro-inositol fraction using a hydrophobic column.

Description

Process for Preparing Chiroinositol Extract from Edible Materials

The present invention relates to a method for preparing an extract of chiroinositol from an edible source. More specifically, the present invention removes sugars other than protein and chiroinositol from water-soluble fractions of edible resources, and acid-decomposes to remove salts or acids of the acid decomposition solution, and performs a hydrophobic column to obtain a chiroinositol fraction. Next, the present invention relates to a method for preparing chiroinositol extract comprising a step of concentrating filtration or drying of the above fraction and a chiroinositol extract prepared from the above method.

Currently, the prevalence of diabetes in Korea varies according to region and researcher, but it reaches about 10% of the highest population and shows a sharp increase of more than 10 times compared to the last 20 years. This aspect is thought to have played a large role in the environmental factors accompanying rapid economic development over the past two decades, and is common to both developed and other developing countries. Therefore, diabetes is expected to become a serious health disorder in most countries in the 21st century.

Diabetes is acute or chronic, such as eye, kidney, cardiovascular, and neurological disorders due to carbohydrate metabolic disorders and accompanying protein and lipid metabolism disorders caused by insulin secretion and insulin function which are essential hormones to keep blood sugar normal. It can be summarized as a chronic disease resulting in a fatal disorder with health complications.

On the other hand, there are two types of diabetes, type I diabetes is called insulin-dependent diabetes mellitus (IDDM), a disease caused by genetic factors that occur mainly in childhood. People with prediabetes rarely produce insulin and die if they do not receive insulin injections. Type II diabetes is called non-insulin-dependent diabetes mellitus (NIDDM), which occurs mainly in adults. 10% of Americans age 65 and older have diabetes, and 90% of all diabetics have type II diabetes. Electrodiabetes is caused by a high level of insulin in the blood but a deficiency in the insulin signaling system. In other words, when insulin binds to the insulin receptor on the cell membrane, glycosylphosphatidyl inositol is decomposed by the action of phospholipase C or phospholipase D to inositol phosphoglycan mediator. It is known to produce. Inositol phosphoglycan mediators activate glucose metabolism by activating pyruvate dehydrogenase phosphatase and glycogen synthase phosphatase, which regulate glucose metabolism. In non-dependent diabetes mellitus, the activation pathways of both enzymes have been found to be defective. At this time, the inositol phosphoglycan mediators found in insulin signaling system generally contain chiroinositol and galactosamine. In the case of insulin-independent diabetes patients, there is a deficiency of chiroinositol in the musculoskeletal and urine, the bloodline of the patient also shows a low content of chiroinositol in the urine, and the lack of chiroinositol is directly related to insulin resistance. (Refer to Saltiel, AR, FASEB. J., 8: 1034-1040, 1994; Larner, J., Endocrine J. , 2: 167-171, 1994; Kennington, AS et al., New Eng J. Med. , 323: 373-378, 1990; Pak, Y et al., J. Biol. Chem. , 267: 16904-16910, 1992; Pak, Y et al., Mol. Cells 8: 301- 309, 1998). In addition, it has been known that blood glucose levels can be normalized by externally administering chiroinositol to such insulin-independent diabetic patients (Ortmeyer, HK et al., Endocrinol. , 132: 640-645, 1993; Fonteles et al. , Diabetologia , 39: 731-734, 1996). Against this background, many people are interested in chiroinositol as a substance capable of treating insulin-independent diabetes. In particular, in recent years, chiroinositol is known to be effective in the treatment of obesity, polycystic ovary, etc. (Nestler JE et al., New Eng. J. Med., 340: 1314-1320, 1999).

As described above, research is being conducted to effectively produce chiroinositol having important physiological activity and use it as a therapeutic agent and a therapeutic aid. Methods for preparing chiroinositol include a method of preparing by extracting and purifying from kasugamycin obtained by fermenting Streptomyces kasugaensis (see US Pat. No. 5,091,596) and a method of preparing by chemical synthesis (see US Patent No. 5,091,596). US Patent No. 5,602,263; US Patent No. 5,516,950) is known.

However, chiroinositol prepared by these methods has a problem in safety due to the residual of toxic starting materials, synthetic precursors and reaction by-products during purification during chemical synthesis to use as a dietary supplement for insulin-independent diabetes patients. The disadvantage is that the production price is high. Therefore, if you develop a method for preparing chiroinositol extract, which is safe from natural edible resources and can lower production costs, it will be very important.

Accordingly, the present inventors have made intensive research efforts to develop a method for preparing chiroinositol extract that can solve the problems of safety and production cost, and have selected raw materials having a high content of chiroinositol by searching for various edible resources that are commonly used as food. From this, it was confirmed that cairoinositol can be manufactured, and this invention was completed.

After all, the main object of the present invention is to provide a method for preparing chiroinositol extract from edible resources.

Another object of the present invention is to provide a chiroinositol extract extracted by the above method.

Method for producing a chiroinositol extract from the edible resources of the present invention is a step of crushing the edible resources and extracted by adding 2 to 6 times the weight of water, and then obtaining a supernatant by centrifugation; Removing the protein by performing a solvent precipitation method or an acid precipitation method on the supernatant, and performing an adsorption column to remove sugars excluding chiroinositol, and then acid-decomposing the acid to obtain an acid decomposition solution; A step of removing the salt or the acid by using caustic soda or an ion exchange resin for the electric acid decomposition solution and obtaining a solution from which the salt or the acid is removed; And performing a hydrophobic column of the liquid from which the electric salt or the acid has been removed to obtain a Cairoinositol fraction, and then concentrated filtration or drying the fraction.

Hereinafter, the method for preparing the chiroinositol extract from the edible resource of the present invention will be described in detail by the process.

Step 1 : Extraction of Edible Resources

The edible resources are crushed and extracted by adding 2-6 times water by weight, and then the supernatant is obtained by centrifugation or filtration: wherein the edible resources are weak beans, soy sauce, soybeans, soybean leaves, soybean germ, skim soybeans, and tofu. Pure water, soy oligosaccharide, soybean pods, bean sprouts, pine needles, pine needles, pine bark or pine sawdust may be used, crushed to pass through a sieve of 10 to 200 mesh, extraction is heated for 10 minutes while heating to 65 to 100 ℃ To 2 hours, and centrifugation is performed at 8,000 to 12,000 rpm for 10 minutes to 1 hour. If the edible resource is a liquid, the crushing process is skipped and the next step after concentration is carried out.

Second Step : Removal of Proteins and Sugars from Extracts

The supernatant was subjected to solvent precipitation or acid precipitation to remove proteins, and an adsorption column was carried out to remove sugars except for chiroinositol, and then acid-decomposed by addition of acid to obtain an acid decomposition solution. The protein is precipitated using silver ethanol, methanol or isopropanol, and the acid precipitation method adjusts the pH to 3.0 to 5.0 by adding acid, and coagulates the protein by heating to 60 to 100 ° C. The electrocoagulated protein is separated using an adsorption column, which is subjected to a column filled with activated carbon or anion exchange resin to obtain a fraction. Acid decomposition is performed at 75 to 120 ° C. for 5 to 48 hours with 2 to 6 N hydrochloric acid.

Third Process : Neutralization

The acidic acid decomposition solution is removed using sodium hydroxide or an ion exchange resin to remove salts or acids and neutralizes. In this case, when neutralized with sodium hydroxide, the cation exchange resin and anion exchange resin column are passed through. Alternatively, the salt may be removed by electrodialysis and neutralized by using an ion exchange resin column, followed by passing through an anion exchange resin and a cation exchange resin column to remove the salt.

Fourth Step : Hydrophobic Column

The electroless salt or acid-free solution is applied to a hydrophobic column to obtain a chiroinositol fraction, and then the electric fraction is concentrated filtered or dried to prepare a chiroinositol: wherein the hydrophobic column is performed by filling a hydrophobic ion exchange resin, Concentrated filtration is concentrated under reduced pressure at 50 to 70 ℃, it is carried out using a filter membrane having a pore size of 0.25 to 0.55㎛. The chiroinositol extract thus prepared varies depending on the type of edible resources, but contains approximately 2.0% or more and 50% or less of chiroinositol.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. .

Example 1 : Selection of high edible resources of Cairoinositol

In order to secure a high content of chiroinositol from various foods and foods, 190 species were analyzed. As a result, most of the samples showed low chiroinositol content of less than 1mg / g sample, and the types of foods containing relatively high amounts of chiroinositol are shown in Table 1.

Chiroinositol content (mg / g dry sample) of representative foods and edible substances Raw material name Content (mg / g) Raw material name Content (mg / g) rice 0.50 wheat 0.17 Sokcheong 1.60 Pasta 0.11 Medicinal beans 2.02 Ginseng 1.05 Soybeans (domestic) 4.36 burdock 0.34 Soybeans (U.S.) 6.75 Sunflower seeds 0.02 Soybeans (Chinese) 4.82 칡 2.32 Bean sprouts 5.20 to 0.45 Bean leaves 8.25 tomato 0.02 Pods 2.59 An ass 0.85 Soybean embryo 7.05 onion 0.09 Tofu 20.00 grape 0.51 Skim soybean 6.45 lemon 0.61 Soy oligosaccharides 17.43 apricot 0.05 Pine bark 4.29 Lime 0.19 pine needles 17.68 honey 0.18 Solsun 17.24 Orange 1.57 Pine sawdust 3.89 leaven 0.12 Pine nuts 1.74 ship 0.05 chicken 0.02 Western plum 0.05 silkworm 1.02 garlic 0.03

Analysis of Cairoinositol was largely divided into four steps: commercially available samples were acid-degraded at 110 ° C. for 48 hours by treating the stock solution with hydrochloric acid so that the final concentration was 6N hydrochloric acid solution; Samples digested with acid were dried using lyophilization or spray drying; As a step of removing impurities contained in the sample and increasing the purity of the chiroinositol, the powdered sample is dissolved in 2 to 5 times distilled water, and centrifuged to take only the supernatant which is a water-soluble component. In order to remove the ions, the cation and anion exchange resins are passed through a carbon-18 Sep-Pak cartridge, and the hydrophobic impurities are adsorbed and removed. The chiroinositol-containing solution passed through the Sepak cartridge was lyophilized to re-powder, suspended in an appropriate amount of distilled water, and then quantified the chiroinositol contained in each sample by HPLC. In order to analyze the electro Cairoinositol by HPLC, each sample was first passed through a 0.2 μm nylon membrane (nylon membrane, 0.2 μm, Costar Spin-x 8169, Cambridge, Mass.). The column used for HPLC was Dionex Carbopak MA-1 (Dionex Carbopak MA-1, U.S.A.) and confirmed by a pulsed electrochemical detector. The buffer used was 60 mM NaOH and eluted for 26 minutes at 0.4 ml per minute. The standard material was injected with 25 μl of myoinositol and chiroinositol prepared at a concentration of 0.018 mg / g. The electrical material had a retention time of a certain time zone and typically maintained a residence time of 3 to 4 minutes. Analytical quantities of all samples were expressed as the average of three experiments.

Example 2 Preparation of Cairoinositol Using Defatted Soybeans

Example 2-1 Preparation of Cairoinositol Using Defatted Soybeans (I)

Degreasing soybeans were pulverized and 800 ml of water was added to 200 g of the powder passed through 20 meshes, followed by hot water extraction at 80 ° C. for 30 minutes. The extract was centrifuged at 10,000 rpm to give 605 ml (8.3% solids, w / v) in defatted soybean solution, and concentrated to 68 ml at 68 ° C. using a vacuum rotary concentrator. 134 g of 12 N hydrochloric acid was added thereto, followed by acid decomposition at 110 ° C. for 48 hours, and 42 ml of 96% NaOH was added to neutralize the acid, which was 236 ml. This was passed through 2.5 L of H ⁺ -Cation Exchange Resin (SK1B, Samyang) in a volume of 60 ml per hour and washed with 5 L of water to obtain 5.2 L of a solution. Subsequently, after passing 2.5 L of OH ^- anion exchange resin (SA20AP, Samyang), washing with 2 L of water gave 7.5 L. 1.4 g of activated carbon and 1 g of diatomaceous earth were added thereto, decolorized and deodorized with stirring at 80 ° C. for 30 minutes, and filtered using a filter press. The filtrate was concentrated under reduced pressure at 60 ° C. to prepare a concentrated solution of 500 ml, frozen at −70 ° C. and freeze-dried to prepare 47 g of a dried product containing 2.8% of chiroinositol.

Example 2-2 Preparation of Cairoinositol Using Defatted Soybean (II)

150 g of pulverized skim soybeans were passed through 20 mesh sieves, and 600 ml of water was added thereto, followed by complete suspension and hydrothermal extraction at 90 ° C. for 20 minutes. 450 ml of the supernatant obtained by centrifugation of the extract was adjusted to pH 4.0 with concentrated hydrochloric acid, and the protein was precipitated while stirring for 10 minutes. 0.9 g of activated carbon and 0.5 g of diatomaceous earth were added thereto, followed by warming at 80 ° C. for 30 minutes. It filtered using the filter press. 310 ml of an electric filtrate (solid content 5.9%, w / v) was concentrated under reduced pressure from 60 ° C. to 73 ml, and 68.4 ml of 35% hydrochloric acid was added thereto to prepare 4.5 N hydrochloric acid solution, followed by acid decomposition at 100 ° C. for 40 hours. I was. The acid-decomposed solution was centrifuged at a speed of 10,000 rpm to remove insoluble foreign matter, that is, precipitates generated after acid decomposition. The supernatant from which the precipitate was removed was passed through 800 mL of OH ^- anion exchange resin (SA20AP, Samyang), and the column was washed with 1.5 L of water to elute the chiroinositol. The eluate was passed through 800 ml of H ⁺ -cation exchange resin (SK1B, Samyang), and the column was washed with 1.5 L of water to elute the chiroinositol. 3,080 ml of this purified solution was passed through 1 L of hydrophobic ion exchange resin (HP20, Samyang) to remove hydrophobic substances in aqueous solution. Thereafter, 3 g of activated carbon and 1.5 g of diatomaceous earth were added thereto, decolorized and deodorized at 80 ° C. for 30 minutes, and filtered using a filter press. The filtrate was concentrated under reduced pressure with a rotary vacuum concentrator at 60 ° C. to make 308 g of a concentrate, frozen at −70 ° C., and freeze-dried to obtain 10.7 g of dried product containing 8.9% of chiroinositol.

Example 2-3 Preparation of Cairoinositol Using Defatted Soybean (III)

120 g of ground skim soybeans were added to 500 ml of water and completely suspended, and then hot water extracted at 90 ° C. for 20 minutes. This was centrifuged to obtain an extract. 360 ml of the extract was adjusted to pH 4.0 using concentrated hydrochloric acid, and the protein was precipitated while stirring for 10 minutes. 0.9 g of activated carbon and 0.5 g of diatomaceous earth were added thereto, warmed at 80 ° C. for 30 minutes, and then filtered using a filter press. . 250 ml of this filtrate (solid content 5.9%, w / v) was concentrated under reduced pressure using a rotary pressure reducer from 60 to 100 ml, and 93.5 ml of 35% hydrochloric acid was added to make 4.5N hydrochloric acid solution. Acid decomposition. The acid-decomposed solution was centrifuged at a speed of 10,000 rpm to remove insoluble foreign matter generated after acid decomposition. The supernatant was neutralized by adding 32.7 g of caustic soda and desalted using an electrodialysis machine. 147 ml of the desalted solution was passed through 50 ml of hydrophobic ion exchange resin (HP20, Samyang) to remove hydrophobic substances in the aqueous solution. Then, 0.2 g of activated carbon and 0.1 g of diatomaceous earth were added, decolorized and deodorized at 80 ° C. for 30 minutes, filtered using a filter press, frozen at −70 ° C., and freeze-dried to obtain 8.7 g of a dried product containing 8.4% of chiroinositol. .

Example 2-4 Preparation of Cairoinositol Using Defatted Soybeans (IV)

Degreased soybeans were pulverized and passed through a 20-mesh sieve to obtain 150 g, which was then added to 600 ml of 80% ethanol solution and evenly suspended, and extracted with sugar components including chiroinositol while stirring at room temperature for 40 minutes. Centrifugation was carried out at 10,000 rpm to separate the extract and the precipitate to obtain 450 ml of supernatant. The precipitate was further added to 600 ml of 80% ethanol solution and evenly suspended, and the sugar components were extracted in the same manner as above. The solution was centrifuged at 10,000 rpm to obtain 585 mL of the supernatant, and the first and second 80% ethanol extracts were mixed. The mixture was ethanol removed using a vacuum concentrator at 60 ° C. to prepare a concentrated solution of 200 ml. Concentrated hydrochloric acid was added to the concentrate to adjust to pH 4.0, and protein was precipitated with stirring for 30 minutes. 0.5 g of activated carbon and 0.3 g of diatomaceous earth were added thereto, warmed with stirring at 80 ° C. for 20 minutes, and filtered through a filter press to remove aggregated protein. 110 mL of concentrated hydrochloric acid was added to 150 mL of the filtrate (solid content 9.5%, w / v), and adjusted to 4 N hydrochloric acid solution, followed by acid decomposition for 38 hours. The acid decomposition solution was filtered using a filter press, followed by passing 1.5 l of OH ^-- anion exchange resin (SA20AP, Samyang) and 1 l of H ⁺ -cation exchange resin (SK1B, Samyang), followed by washing with 3 l of water. A solution containing chiranoinositol was obtained. 2,080 ml of this purified solution was passed through 1.2 L of hydrophobic ion exchange resin (HP20, Samyang) to remove hydrophobic substances. 4 g of activated carbon and 2 g of diatomaceous earth were added to the solution passed through the hydrophobic column, stirred at 80 ° C. for 30 minutes, and filtered using a filter press. The solution was concentrated under reduced pressure at 60 ° C. or lower using a vacuum rotary condenser to make 203 ml of concentrated solution, and then frozen at −70 ° C. and freeze-dried to obtain 7.5 g of dried product containing 12.5% of chiroinositol.

Example 3 Preparation of Cairoinositol Using Pure Tofu

Concentrated under reduced pressure until 3,000 ml of tofu pure water (liquid containing water-soluble components of red bean produced by pressing and coagulant during tofu production) became 250 ml (30% of solid content, w / v) at 60 ℃. Then, concentrated hydrochloric acid was added to adjust pH to 4.0, and then warmed at 80 ° C. for 10 minutes to aggregate the protein. 0.5 g of activated carbon and 0.2 g of diatomaceous earth were added to the solution, and the mixture was stirred for 30 minutes while being heated to 80 ° C. and filtered through a filter press. 220 ml (29% solids, w / v) of the electric filtrate was passed through a column containing 110 g of activated carbon and washed with 500 ml of water, thereby obtaining 647 ml of a liquid obtained by partially adsorbing sugars other than Cairoinositol. 540 ml of 35% hydrochloric acid was added to the solution, followed by acid decomposition at 90 캜 for 40 hours. The acid-decomposed liquid was filtered using a filter press, and then 5 L of OH ^-- anion exchange resin (SA20AP, Samyang) and 3 L of H ⁺ -cation exchange resin (SK1B, Samyang) were sequentially passed. Thus, 10.6 L of the purified solution through the ion exchange resin was passed through 2.5 L of hydrophobic ion exchange resin (HP20, Samyang) to remove hydrophobic substances. 20 g of activated carbon and 10 g of diatomaceous earth were added thereto, decolorized and deodorized with stirring at 80 ° C. for 30 minutes, and filtered by a filter press. The filtered 12.8 L was spray dried at a blowing temperature of 167 ° C. and a blowing air temperature of 90 ° C. to obtain 51 g of an extract containing 2.5% of chiroinositol.

Example 4 Preparation of Chiroinositol Using Pine Needle

1,000 g of pine needles were washed with water, and then crushed using Chopper. 4 liters of water was added thereto, and hot water was extracted for 1 hour with stirring at 60 ° C. Got. 5.2 g of activated carbon and 2.7 g of diatomaceous earth were added thereto, followed by warming at 80 ° C. for 20 minutes, followed by centrifugation at 10,000 rpm to obtain 2,010 ml of a supernatant (1.2% solids, w / v). This supernatant was passed through 5 liters of anion exchange resin (SA20-OH, Samyang) together with 5 liters of water, and 6,840 ml of sugar was removed except for chiroinositol. It was. 412 mL of 35% hydrochloric acid was added to the concentrate, and adjusted to 6N, followed by acid decomposition at 110 ° C for 48 hours. The acid-decomposed liquid was filtered using a filter press, and the filtrate was passed through 5.5 L of OH ^-- anion exchange resin (SA20AP, Samyang) and 3 L of H ⁺ -cation exchange resin (SK1B, Samyang). The column was washed with 10 liters of water to recover the solution containing chiranoinositol. 10.2 L of the electrolytic solution was passed through 3 L of hydrophobic ion exchange resin (HP20, Samyang) to remove the hydrophobic material in the aqueous solution. 20 g of activated carbon and 10 g of diatomaceous earth were added thereto, decolorized and deodorized with stirring at 80 ° C. for 30 minutes, and filtered through a filter press. 9,840 ml of the electric filtrate was spray-dried at a blowing temperature of 165 캜 and an air blowing temperature of 87 캜 to obtain 41 g of an extract containing 8.2% of chiroinositol.

As described and demonstrated in detail above, the present invention searches for a variety of edible resources that are commonly used as foods to select a raw material having a high content of chiroinositol, and provides a method for producing a chiroinositol extract therefrom. The chiroinositol extract prepared by the present invention has no problem in safety because it is prepared by using an edible resource as a raw material, the content of the chiroinositol can be supplied cheaply in terms of cost, a therapeutic agent for diabetics, Dietary supplements, beverages, confectionery, bakery, ice cream, chewing gum and other general food materials can be usefully used.

Claims (13)

(Iii) crushing the edible resources, extracting by adding 2-6 times water by weight, and then obtaining a supernatant by centrifugation or filtration; (Ii) removing the protein by performing the solvent superposition method or the acid precipitation method on the supernatant, and removing the sugar except the chiroinositol by applying the adsorption column, and then acid-decomposing by adding an acid to obtain an acid decomposition solution; (Iii) neutralizing the electric acid decomposition liquid by using caustic soda or ion exchange resin to remove salt or acid; And, (Iii) A method for producing a chiroinositol extract from an edible resource, comprising applying an electric salt or acid-free solution to a hydrophobic column to obtain a chiroinositol fraction, and then concentrating or drying the electric fraction. The method of claim 1, Edible resources include medicinal beans, sokcho, soybean, soybean leaves, soybean germ, skim soybean, Soy oligosaccharides, pods, bean sprouts, pine needles, pine needles, pine bark or pine Characterized by sawdust Method for producing a chiroinositol extract from edible resources. The method of claim 1, Shredding can pass through a sieve of 10 to 200 mesh Pulverizing Method for producing a chiroinositol extract from edible resources. The method of claim 1, Extraction is carried out for 10 minutes to 2 hours with heating to 65-100 ° C. Characterized in that Method for producing a chiroinositol extract from edible resources. The method of claim 1, Solvent precipitation involves the use of ethanol, methanol or isopropanol to Characterized in that settling Method for producing a chiroinositol extract from edible resources. The method of claim 1, In acid precipitation, pH is adjusted to 3.0 to 5.0 by adding acid, and 60 to Characterized in that the protein is solidified by heating to 100 ℃ Method for producing a chiroinositol extract from edible resources. The method of claim 1, Adsorption column is performed by filling activated carbon or anion exchange resin. Characterized Method for producing a chiroinositol extract from edible resources. The method of claim 1, Acid decomposition was performed at 75 to 100 ° C. using 2 to 6 N hydrochloric acid. Characterized in that performed for 48 hours Method for producing a chiroinositol extract from edible resources. The method of claim 1, When neutralizing with caustic soda, cation exchange resin and Passing through the anion exchange resin column in turn or using electrodialysis Characterized by removing salts Method for producing a chiroinositol extract from edible resources. The method of claim 1, In the case of neutralization using an ion exchange resin column, It is characterized by removing the salt by passing through the cation exchange resin column in sequence doing Method for producing a chiroinositol extract from edible resources. The method of claim 1, Hydrophobic columns are filled with hydrophobic ion exchange resins. Characterized Method for producing a chiroinositol extract from edible resources. The method of claim 1, Concentrated filtration is concentrated under reduced pressure at 50 to 70 ℃, pore size 0.25 to Characterized in that it is carried out using a filtration membrane of 0.55㎛ Method for producing a chiroinositol extract from edible resources. Crushed edible resources and extracted by adding 2 to 6 times by weight of water, and then obtained a supernatant by centrifugation or filtration, and then remove the protein by solvent precipitation or acid precipitation, applied to the adsorption column To remove the sugar except for chiroinositol, and then acid-decomposed to give an acid-decomposing solution, to which a salt or acid-free solution obtained by adding caustic soda or using an ion exchange resin was applied to a hydrophobic column. A chiroinositol extract having a content of cairoinositol of 2.0 to 50%, obtained by obtaining a cairoinositol fraction, followed by concentrated filtration or drying of the above fraction.