KR20010083600A - Process for Preparing Pullulan Employing Waste Materials Containing High Concentration of Sugars from Food-processing Industry - Google Patents
Process for Preparing Pullulan Employing Waste Materials Containing High Concentration of Sugars from Food-processing Industry Download PDFInfo
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
- C12P19/10—Pullulan
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12N2500/34—Sugars
Abstract
Description
본 발명은 식품가공 부산물을 이용한 고분자 중합체 풀루란의 제조방법에 관한 것이다. 좀 더 구체적으로, 본 발명은 탄소원으로서 고농도 당성분을 포함하는 식품가공 부산물인 밥당화폐액, 엿기름 당화폐액 또는 사과박을 배지에 첨가하는 공정을 포함하는 고분자 중합체 풀루란의 제조방법에 관한 것이다.The present invention relates to a method for producing a polymeric polymer pullulan using food processing by-products. More specifically, the present invention relates to a method for producing a high molecular polymer pullulan comprising the step of adding to the medium, rice saccharin liquid, malt saccharin liquid or apple foil as a food processing by-product containing a high concentration of sugar components as a carbon source.
대부분의 미생물들은 전분 또는 포도당과 같은 탄소원과 효모 추출액, 염화암모늄, 폐당밀 등의 질소원을 포함하는 영양원과 적당한 환경이 조성될 경우, 생육을 시작하며, 그 중에는 우리 생활에 필요한 고분자 중합체를 생산하는 미생물들도 있다. 미생물이 생산하는 고분자 중합체로는 풀루란(pullulan), 잔탄(xanthan), 커드란(curdlan), 젤란(gellan) 등의 교질화제, 유화제, 안정제 및 젤이 이에 속하며, 식품산업 및 화공산업에서 널리 사용되고 있다. 이와 같은 고분자 중합체는 국내에서 생산되지 않고, 전량을 외국에서 수입하고 있는데, 1997년의 수입액은 약 800억원 정도이었으며, 식품의 질을 향상시키기 위하여 필수 불가결한 물질이므로 수입량은 계속 증가할 것이다.Most microorganisms start growing when a suitable environment and a nutrient source including a carbon source such as starch or glucose and a nitrogen source such as yeast extract, ammonium chloride and waste molasses are created, among which are produced polymer polymers necessary for our lives. There are also microbes. Polymers produced by microorganisms include gelling agents, emulsifiers, stabilizers and gels such as pullulan, xanthan, curdlan and gellan, and are widely used in the food and chemical industries. It is used. These polymers are not produced in Korea, and the total amount is imported from foreign countries. In 1997, the amount of import was about 80 billion won, and the amount of imports will continue to increase as it is indispensable to improve the quality of food.
특히, 식품 및 화학공업에 필요한 풀루란은 미국 및 일본에서 전량 수입하여 사용하고 있는데, 이는 풀루란의 생산비용이 외국에서 수입하는 비용보다 비싸기 때문이다. 따라서, 풀루란을 생산하는 기술을 획기적으로 개발하여 생산단가를 보다 더 낮추거나, 보다 저렴한 배지를 사용하여 직접 생산비를 낮추어야 할 것이나, 이에 대한 국내의 기술은 전무한 실정이다.In particular, the full amount of pullulan required for the food and chemical industry is imported and used in the United States and Japan, because the production cost of pullulan is higher than that imported from abroad. Therefore, it is necessary to develop the technology to produce pullulan significantly and lower the production cost or lower the production cost by using a cheaper medium, but there is no domestic technology for this.
한편, 필요한 산물의 생산에 부가적으로 생산되는 환경 오염성 폐기물 및 부산물의 이용에 대한 연구는 환경 및 자원의 재활용적 측면에서 많은 연구자들에 의하여 진행되어 왔다. 전기 식품가공 부산물 중, 고농도 당성분을 포함하는 것으로서 전통음료 중의 하나인 식혜와 사과쥬스의 대량 생산과정에서 필수 불가결하게 발생하는 고농도 당성분을 포함하는 부산물 즉, 밥당화폐액, 엿기름 당화폐액 및 사과박이 있다. 식혜 및 사과쥬스의 대량생산 공정에서 생산규모에 따라 1일에 10 내지 20톤 이상 발생되는 전기 고농도 당성분을 포함하는 부산물의 전체 당 농도는 약 20%가 되기 때문에, 일반적인 정화시설 및 방법으로는 정화가 불가능하여 특수 정화업체에 의뢰하고 있는 실정이다. 따라서, 식품가공산업의 폐기물 및 부산물을미생물의 영양원으로 개발하여 고분자 중합체를 생산할 수 있다면, 이는 환경오염의 원천적인 방지와 이를 이용한 고부가가치의 산물을 생산한다는 측면에서 매우 중요한 의미를 지닌다고 할 것이다.On the other hand, the use of environmental pollutants and by-products produced in addition to the production of the required product has been conducted by many researchers in terms of recycling of the environment and resources. By-products containing high concentrations of sugar components, including by-products containing high concentrations of sugar, which are indispensable in the mass production of Sikhye and apple juice, which are one of the traditional beverages. There is Park. In the mass production process of Sikhye and apple juice, the total sugar concentration of by-products including the electric high concentration sugar component that is generated more than 10 to 20 tons per day is about 20%. As it is impossible to purify, it is requested by a special purifier. Therefore, if the waste and by-products of the food processing industry can be developed as a microbial nutrient source to produce a polymer, this is very important in terms of preventing the source of environmental pollution and producing high value products using the same.
이에, 본 발명자들은 경제적으로 풀루란을 제조할 수 있는 방법을 개발하고자 예의 연구노력한 결과, 식품가공 부산물로서 전통식품 중의 하나인 식혜의 대량생산과정과 사과를 착즙하여 사과쥬스를 생산하는 공정에서 필수 불가결하게 발생하는 고농도 당성분을 포함하는 부산물 즉, 밥당화폐액, 엿기름 당화폐액 및 사과박을 탄소원으로 사용하여, 경제적으로 풀루란을 제조할 수 있음을 확인하고, 본 발명을 완성하게 되었다.Accordingly, the present inventors have made intensive studies to develop a method for producing pullulan economically, and as a result of food processing by-products, it is essential in the mass production process of Sikhye, one of the traditional foods, and in the process of producing apple juice by extracting apples By using a by-product containing a high concentration of sugar components that is indispensable, that is, rice saccharin, malt saccharin and apple foil as a carbon source, it was confirmed that the pullulan can be economically produced, to complete the present invention.
결국, 본 발명의 주된 목적은 고농도 당성분을 포함하는 식품가공 부산물을 이용한 고분자 중합체 풀루란의 제조방법을 제공하는 것이다.After all, the main object of the present invention is to provide a method for producing a polymeric polymer pullulan using a food processing by-product containing a high concentration of sugar components.
도 1은 본 발명의 풀루란의 제조방법을 도식적으로 나타낸 공정도이다.1 is a process diagram schematically showing a method for producing pullulan of the present invention.
도 2는 탄소원에 따른 본 발명의 풀루란의 생산 수율을 나타낸 그래프이다.2 is a graph showing the yield of the pullulan of the present invention according to the carbon source.
본 발명의 식품가공 부산물을 이용한 고분자 중합체 풀루란의 제조방법은 풀루란을 생산하는 미생물을 탄소원으로서 고농도 당성분을 포함하는 식품가공 부산물이 함유된 배지에 접종하여, 20 내지 40℃에서 150 내지 200rpm으로 55 내지 85시간 동안 배양한 종균 배양액을 전기 배지에 2 내지 8%(v/v)로 접종하고, 20 내지40℃에서 150 내지 200rpm으로 3 내지 7일 동안 배양하여 배양액을 수득하는 공정; 전기 수득된 배양액을 원심분리하여 얻은 상등액에 유기용매를 가하여 추출하고, 전기 추출액을 원심분리하여 침전물을 수득하는 공정; 및, 전기 침전물을 증류수에 용해시킨 후, 투석하여 투석 내부분획을 수득하고, 전기 투석 내부분획을 진공 동결건조하는 공정을 포함한다.The method for producing a polymeric polymer pullulan using the food processing by-product of the present invention is inoculated into a medium containing a food processing by-product containing a high concentration of sugar components as a carbon source, the microorganisms producing pullulan, at 150 to 200 rpm at 20 to 40 ℃ Inoculating 2 to 8% (v / v) of the spawn culture medium incubated for 55 to 85 hours in an electric medium, and incubating at 20 to 40 ° C. at 150 to 200 rpm for 3 to 7 days to obtain a culture solution; Extracting by adding an organic solvent to the supernatant obtained by centrifuging the previously obtained culture, and centrifuging the extract to obtain a precipitate; And dissolving the electric precipitate in distilled water, followed by dialysis to obtain an internal dialysis fraction, and vacuum freeze drying the electrodialysis internal fraction.
이하, 본 발명의 식품가공 부산물을 이용한 고분자 중합체 풀루란의 제조방법을 공정별로 나누어 구체적으로 설명하고자 한다.Hereinafter, a method for preparing a polymer polymer pullulan using the food processing by-product of the present invention will be described in detail by process.
제 1공정: 배양액의 수득 First step : obtaining culture
풀루란을 생산하는 미생물을 탄소원으로서 고농도 당성분을 포함하는 식품가공 부산물이 함유된 배지에 접종하여, 20 내지 40℃에서 150 내지 200rpm으로 55 내지 85시간 동안 배양한 종균 배양액을 전기 배지에 2 내지 8%(v/v)로 접종하고, 20 내지 40℃에서 150 내지 200rpm으로 3 내지 7일 동안 배양하여 배양액을 수득한다: 이때, 풀루란을 생산하는 미생물은 당업계에서 통상적으로 사용되는 균주를 사용할 수 있으나, 에어로바시디움 풀루란스(Aerobasidium pullulans)가 바람직하다. 또한, 전기 식품가공 부산물로서는 밥당화폐액, 엿기름 당화폐액 또는 사과박을 전체 배지성분에 대하여 1 내지 10%(w/v 또는 v/v)로 하여 사용하며, 배지는 3 내지8g/L의 K2HPO4,0.01 내지 1g/L의 MgSO4ㆍ7H2O, 0.3 내지 1.5g/L의 (NH4)2SO4, 0.5 내지 3.0g/L의 NaCl 및 1.0 내지 5.0g/L의 효모 추출물이 포함된 것을 사용한다.A microorganism producing pullulan was inoculated into a medium containing a food processing by-product containing a high concentration of sugar as a carbon source, and the seed culture was incubated at 20 to 40 ° C. at 150 to 200 rpm for 55 to 85 hours in an electric medium. Inoculate at 8% (v / v) and incubate at 20 to 40 ° C. for 3 to 7 days at 150 to 200 rpm to obtain a culture solution: wherein the microorganisms producing pullulan are prepared from strains commonly used in the art. Although used, Aerobasidium pullulans is preferred. In addition, as an electric food processing by-product, rice saponification liquid, malt saccharification liquid, or apple foil is used as 1 to 10% (w / v or v / v) with respect to the whole medium component, and the medium is K of 3-8 g / L. 2 HPO 4, 0.01-1 g / L MgSO 4 .7H 2 O, 0.3-1.5 g / L (NH 4 ) 2 SO 4 , 0.5-3.0 g / L NaCl and 1.0-5.0 g / L yeast extract Use this included.
제 2공정: 배양액의 추출 Second Step : Extraction of Culture Solution
전기 수득된 배양액을 원심분리하여 얻은 상등액에 유기용매를 가하여 추출하고, 전기 추출액을 원심분리하여 침전물을 수득한다: 이때, 원심분리는 5000 내지 12000×g의 범위에서 10 내지 30분 동안 실시하고, 유기용매는 에탄올 또는 이소프로판올을 사용한다.The obtained supernatant was extracted by adding an organic solvent to the supernatant obtained by centrifugation, and the extract was centrifuged to obtain a precipitate: the centrifugation was carried out for 10 to 30 minutes in the range of 5000 to 12000 × g, The organic solvent uses ethanol or isopropanol.
제 3공정: 풀루란의 제조 Third Step : Preparation of Pullulan
전기 침전물을 증류수에 용해시킨 후, 투석하여 투석 내부분획을 수득하고, 전기 투석 내부분획을 진공 동결건조한다: 이때, 투석은 배제 분자량이 10,000 내지 15,000인 투석막을 사용하여 1 내지 5일 동안 증류수를 3 내지 7회 교체함으로써 수행한다.The electric precipitate is dissolved in distilled water, followed by dialysis to obtain an internal dialysis fraction, and the electrodialysis internal fraction is vacuum lyophilized: dialysis is performed by distilled water for 1 to 5 days using a dialysis membrane having an exclusion molecular weight of 10,000 to 15,000. This is done by replacing 3-7 times.
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로, 본 발명의 요지에따라 본 발명의 범위가 이들 실시예에 의해 제한되지 않는다는 것은 당업계에서 통상의 지식을 가진 자에 있어서 자명할 것이다.Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. .
실시예 1: 고농도 당성분을 포함하는 식품가공 부산물의 성분분석 Example 1 Component Analysis of Food Processing By-Products Containing High Concentration Sugar
실시예 1-1: 밥당화폐액과 엿기름 당화폐액의 성분분석 Example 1-1 : Component Analysis of Rice Saccharin and Malt Saccharin
전통음료인 식혜의 대량생산 공정에서 발생하는 고농도 당성분을 포함하는 부산물인 밥당화폐액과 엿기름 당화폐액의 성분을 액체 크로마토그래피를 사용하여 분석하였다. 액체 크로마토그래피는 굴절률 검출기(Model 410 refractive index detector, Waters 600E system, U.S.A.)가 장착되고, 겔여과 컬럼(Shodex KB800M, Shodex KM805, Waters, U.S.A.)이 두 개 연결된 것을 사용하였다. 시료의 농도와 주입 양은 각각 5.0mg/㎖와 100㎕이었으며, 시료의 이동속도는 분당 0.5㎖이었다. 액체 크로마토그래피를 이용한 분석 결과, 밥당화폐액과 엿기름 당화폐액의 전체 당농도는 약 20%이었다. 표 1은 밥당화폐액과 엿기름 당화폐액의 구성성분을 나타낸 것이다. 표 1에서 보듯이, 밥당화폐액의 주성분은 덱스트린, 말토트리오스 및 말토오스이었으며, 엿기름 당화폐액의 주성분은 덱스트린과 말토오스이었다.The components of rice saccharin and malt saccharin, which are by-products containing high concentrations of sugar components, in the mass production process of Sikhye, a traditional beverage, were analyzed using liquid chromatography. Liquid chromatography was equipped with a refractive index detector (Model 410 refractive index detector, Waters 600E system, U.S.A.) and two gel filtration columns (Shodex KB800M, Shodex KM805, Waters, U.S.A.) were connected. The concentration and injection amount of the sample were 5.0 mg / ml and 100 µl, respectively, and the moving speed of the sample was 0.5 ml per minute. As a result of analysis using liquid chromatography, the total sugar concentration of the rice saccharin and malt saccharin was about 20%. Table 1 shows the components of the rice sugar and the malt saccharin liquid. As shown in Table 1, the main components of the rice saccharin was dextrin, maltotriose and maltose, and the main components of malt saccharin was dextrin and maltose.
실시예 1-2: 사과박의 성분분석 Example 1-2 : Component Analysis of Apple Foil
사과쥬스의 대량생산 공정에서 발생하는 고농도 당성분을 포함하는 부산물인 사과박의 성분을 검정된 식품분석법(식품분석, 김경삼 외, 효일문화사, 1999)에 의하여 분석하였다. 사과를 착즙하여 사과쥬스를 생산한 후, 부산물로 생성되는 사과박의 수분 함량은 5.8%이었으며, 각각의 구성성분은 표 2에 나타내었다.The components of apple gourd, which is a by-product containing high concentration of sugar components, which were produced in the mass production process of apple juice, were analyzed by a validated food analysis method (food analysis, Kim Kyung-sam et al., Hyoil Cultural History, 1999). After the apples were juiced to produce apple juice, the moisture content of the apple juice produced as a by-product was 5.8%, and each component is shown in Table 2.
실시예 2: 탄소원으로서 밥당화폐액을 이용한 풀루란의 제조 Example 2 Preparation of Pullulan Using Rice Glucose Solution as Carbon Source
풀루란을 생산하기 위한 배지의 조성은 5g/L의 K2HPO4, 0.2g/L의 MgSO4ㆍ7H2O, 0.6g/L (NH4)2SO4, 1.0g/L의 NaCl 및 2.5g/L 효모추출물로 구성되었고, 탄소원으로 밥당화폐액을 멸균한 후 멸균된 배지에 무균적으로 3%(v/v)으로 혼합하여 사용하였다. 전배양은 고체 배지에서 일정시간 배양한 에어로바시디움 풀루란스(Aerobasidium pullulans, ATCC 42023)을 한 백금니 취하여 250㎖ 용량의 플라스크에 멸균하여 준비된 50㎖의 배지에 접종한 후, 30℃에서 180rpm의 진탕 속도로 72시간 진탕배양하였다. 본배양은 전배양한 배양액을 500㎖ 용량의 플라스크에 멸균되어 준비된 150㎖의 동일 배지에 5%(v/v)를 접종하여 전배양과 동일한 방법으로 5일간 배양하였다. 전기 배양액을 8000×g에서 20분간 원심분리하여 침점물을 제거한 상등액을 회수하였다. 전기 회수한 상등액에 2배 용량의 95% 에탄올을 첨가하고, 잘 혼합하여 4℃에서 하룻밤 동안 방치하였다. 이 용액을 8000×g에서 20분간 원심분리하고 상등액을 제거한 후, 침전물을 95% 에탄올로 2 내지 3회 세척하였다. 세척한 침전물을 적당한 양의 증류수에 용해시킨 다음, 2 내지 3일 동안에 4내지 5회 증류수를 바뀌어 주면서 투석하여 염성분을 포함한 저분자 물질을 제거하였다. 사용된 투석막은 배제 분자량 12,000∼14,000인 투석막을 사용하였으며, 투석 후 풀루란을 진공 동결건조하여 건조물을 회수하였다.The composition of the medium for producing pullulan was 5 g / L K 2 HPO 4 , 0.2 g / L MgSO 4 .7H 2 O, 0.6 g / L (NH 4 ) 2 SO 4 , 1.0 g / L NaCl and It was composed of 2.5g / L yeast extract, sterilized rice glycosylated liquor with a carbon source and used aseptically mixed 3% (v / v) in the sterilized medium. The preculture was inoculated in a 50 ml medium prepared by sterilizing a 250 ml volumetric flask with Aerobasidium pullulans (ATCC 42023) incubated in a solid medium for a predetermined time, and then inoculating it into a 50 ml medium prepared at 30 ° C for 180 rpm. 72 hours shaking culture at shaking speed. The main culture was inoculated 5% (v / v) in the same medium of 150ml prepared by sterilizing the culture medium pre-cultured in a 500ml flask was incubated for 5 days in the same manner as the preculture. The supernatant was removed by centrifugation of the culture medium at 8000 x g for 20 minutes to remove the deposits. Two times of 95% ethanol was added to the recovered supernatant, mixed well, and left overnight at 4 ° C. The solution was centrifuged at 8000 × g for 20 minutes and the supernatant was removed, then the precipitate was washed 2-3 times with 95% ethanol. The washed precipitate was dissolved in an appropriate amount of distilled water, and then dialyzed with changing distilled water four to five times in 2-3 days to remove low molecular weight substances including salt components. The dialysis membrane used was a dialysis membrane having an exclusion molecular weight of 12,000 to 14,000. After dialysis, the pullulan was lyophilized in vacuo to recover the dried product.
실시예 3: 탄소원으로서 엿기름 당화폐액을 이용한 풀루란의 제조 Example 3 Preparation of Pullulan Using Malt Saccharification Waste as Carbon Source
탄소원으로서 엿기름 당화폐액을 이용한다는 것을 제외하고는, 실시예 1의 제조방법과 동일한 방법으로 풀루란을 제조하였다.Pullulan was prepared in the same manner as in Preparation Example 1, except that malt saccharification liquid was used as the carbon source.
실시예 4: 탄소원으로서 사과박을 이용한 풀루란의 제조 Example 4 Preparation of Pullulan Using Apple Foil as a Carbon Source
탄소원으로서 사과박을 이용한다는 것을 제외하고는, 실시예 1의 제조방법과 동일한 방법으로 풀루란을 제조하였다.Pullulan was prepared in the same manner as in Preparation Example 1, except that apple foil was used as the carbon source.
비교실시예 1: 탄소원으로서 포도당을 이용한 풀루란의 제조 Comparative Example 1 : Preparation of pullulan using glucose as a carbon source
탄소원으로서 포도당을 이용한다는 것을 제외하고는, 실시예 1의 제조방법과 동일한 방법으로 풀루란을 제조하였다.Pullulan was prepared in the same manner as in Preparation Example 1, except that glucose was used as the carbon source.
실시예 5: 제조된 풀루란의 분석 Example 5 Analysis of Prepared Pullulan
실시예 2, 3, 4 및 비교실시예 1에서 제조된 풀루란을 박막 크로마토그래피(thin layer chromatography, TLC)와 가스 크로마토그래피(gaschromatography, GC)를 사용하여 분석하였다. 전기 실시예 및 비교실시예에서 제조한 풀루란을 1N HCl을 사용하여 100℃에서 2시간 가수분해시킨 후, 박막 크로마토그래피를 실시하였으며, 시판되는 풀루란(Sigma Co., U.S.A.)을 대조구로 사용하였다. 박막 크로마토그래피의 전개용매는 부탄올, 피리딘 및 증류수를 6:4:3의 비율(v/v/v)로 혼합한 용액을 사용하였으며, 아닐린(aniline)과 디페닐아민(diphenylamine)을 각각 1%(v/v)의 농도로 아세톤에 만든 용액(5㎖)과 85%(v/v) 인산용액(1㎖)을 혼합하여 만든 용액을 발색시약으로 사용하여 전개된 풀루란을 확인하였다. 아울러, 가스 크로마토그래피(GC Model 5890, Hewlett-Packard, U.S.A.)는 헬륨을 이동상으로 사용하고, 주입속도는 1.2㎖/분, 압력은 16.5기압으로 진행하였다. 분석은 시료 주입시 200℃로 3분 동안 유지시키고, 분당 8℃씩 증가시켜 280℃에서 실시하였으며, 시판되고 있는 풀루란을 대조구로 사용하여 확인하였다.Pullulan prepared in Examples 2, 3, 4 and Comparative Example 1 was analyzed using thin layer chromatography (TLC) and gas chromatography (gas chromatography). The pullulan prepared in the above Examples and Comparative Examples were hydrolyzed at 100 ° C. for 2 hours using 1N HCl, followed by thin layer chromatography, and commercially available pullulan (Sigma Co., USA) was used as a control. It was. Thin-film chromatography was used as a solvent in which butanol, pyridine and distilled water were mixed at a ratio of 6: 4: 3 (v / v / v), and 1% of aniline and diphenylamine, respectively. The developed pullulan was identified using a solution prepared by mixing acetone (5 ml) and 85% (v / v) phosphate solution (1 ml) at a concentration of (v / v) as a color developing reagent. In addition, gas chromatography (GC Model 5890, Hewlett-Packard, U.S.A.) used helium as a mobile phase, the injection rate was 1.2ml / min, the pressure was 16.5 atm. The analysis was maintained at 200 ° C. for 3 minutes at the time of sample injection, and was performed at 280 ° C. by 8 ° C. per minute, and confirmed using commercial pullulan as a control.
실시예 6: 풀루란의 생산성 비교 Example 6 Productivity Comparison of Pullulan
실시예 2, 3, 4 및 비교실시예 1에서 제조된 풀루란의 생산성 결과를 표 3과 도 2에 나타내었다. 표 3 및 도 2에서 보듯이, 밥당화폐액은 종래의 풀루란 생산 배지의 탄소원인 포도당의 경우보다 높은 생산성을 나타내었다. 이는 전통음료인 식혜의 대량생산 공정에서 필수 불가결하게 발생하는 환경오염성 부산물인 밥당화폐액이 풀루란 생합성의 최적 탄소원으로 사용할 수 있다는 것이며, 이와 같은 결과는 풀루란을 생산하기 위한 직접 생산비를 50% 이상 절감할 수 있음을 의미한다. 또한, 풀루란의 생산성 증가에 따른 생산 설비와 간접 생산비의 절약 및 환경 오염물질의 정화에 소요되는 비용의 감소까지를 고려한다면, 대체할 수 있는 포도당의 원가 이상의 생산비를 절감하는 결과이다. 아울러, 풀루란 생합성을 위하여, 탄소원으로 사용한 엿기름 당화폐액과 사과박의 경우, 풀루란의 생산성은 포도당을 사용하였을 때 보다 다소 낮지만, 포도당의 원가와 생산성을 동시에 고려해보면, 비교적 우수한 탄소원으로 사용할 수 있다는 결론을 얻었으므로 이 역시, 직접 생산비의 절감과 환경오염성 물질의 완전한 제거라는 효과를 기대할 수 있다.The productivity results of the pullulan prepared in Examples 2, 3, 4 and Comparative Example 1 are shown in Table 3 and FIG. As shown in Table 3 and Figure 2, the rice sugar liquid showed a higher productivity than the case of glucose which is a carbon source of the conventional pullulan production medium. This means that rice sugar, an environmental pollutant by-product that is indispensable in the mass production process of Sikhye, a traditional beverage, can be used as an optimal carbon source for pullulan biosynthesis, and this result is 50% of the direct production cost for producing pullulan. That means you can save more. In addition, considering the reduction in production equipment and indirect production costs and the cost of purifying environmental pollutants due to the increase in the productivity of pullulan, it is a result of reducing the production cost more than the cost of replaceable glucose. In addition, for the production of pullulan biosynthesis, in the case of malt saccharification liquid and apple peel, which are used as carbon sources, the productivity of pullulan is slightly lower than that of using glucose, but considering the cost and productivity of glucose simultaneously, it can be used as a relatively excellent carbon source. It is also possible to expect the effect of direct reduction of production costs and complete elimination of environmental pollutants, since it can be concluded.
이상에서 상세히 설명하고 입증하였듯이, 본 발명은 탄소원으로서 고농도 당성분을 포함하는 식품가공 부산물인 밥당화폐액, 엿기름 당화폐액 또는 사과박을 배지에 첨가함으로써 고분자 중합체 풀루란을 경제적으로 제조하는 방법을 제공한다. 본 발명에 의하면, 풀루란의 제조시 탄소원으로서 사용되는 포도당을 식품가공 부산물인 밥당화폐액, 엿기름 당화폐액 또는 사과박으로 대체하여 사용하기 때문에 직접생산비의 50%를 절감할 수 있으며, 특히, 탄소원으로서 밥당화폐액을 사용할 경우에는 풀루란의 생산성 향상을 도모할 수 있다. 뿐만 아니라, 풀루란 제조시 고농도의 당성분을 포함하는 식품가공 부산물을 탄소원으로 사용하기 때문에, 환경오염원의 정화를 위한 처리비용을 절감시킬 수 있는 효과를 얻을 수 있다.As described and demonstrated in detail above, the present invention provides a method for economically manufacturing a polymeric polymer pullulan by adding a food sugar by-product, a maltose saccharin solution, or an apple leaf, which are by-products containing a high concentration of sugar as a carbon source, to the medium. do. According to the present invention, since the glucose used as a carbon source in the production of pullulan is replaced with a food sugar by-product, rice malt, or sake, it is possible to reduce 50% of direct production costs. When the rice sugar liquid is used, the productivity of pullulan can be improved. In addition, since pullulan is used as a carbon source by using a food processing by-product containing a high concentration of sugar components, it is possible to reduce the treatment cost for the purification of environmental pollution sources.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| KR100408320B1 (en) * | 2001-02-09 | 2003-12-01 | (주)케이비피 | Process for Preparing PS-7 (heteropoly- saccharide-7) Employing Waste Materials Containing High Concentration of Sugars from Food-processing Industry |
| KR100414952B1 (en) * | 2001-02-09 | 2004-01-14 | (주)케이비피 | Process for Preparing of Pullulan Employing By-product from Soybean Source Fermentation |
| KR100739022B1 (en) * | 2005-12-12 | 2007-07-12 | 동아대학교 산학협력단 | Method for continuous production of pullulan with feeding of the sucrose |
| US10568839B2 (en) | 2011-01-11 | 2020-02-25 | Capsugel Belgium Nv | Hard capsules |
| US11319566B2 (en) | 2017-04-14 | 2022-05-03 | Capsugel Belgium Nv | Process for making pullulan |
| US11576870B2 (en) | 2017-04-14 | 2023-02-14 | Capsugel Belgium Nv | Pullulan capsules |
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| KR100937489B1 (en) | 2007-10-10 | 2010-01-19 | 신달하 | Preparation method of pullulan using fermented material of soybean curd residue |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100408320B1 (en) * | 2001-02-09 | 2003-12-01 | (주)케이비피 | Process for Preparing PS-7 (heteropoly- saccharide-7) Employing Waste Materials Containing High Concentration of Sugars from Food-processing Industry |
| KR100414952B1 (en) * | 2001-02-09 | 2004-01-14 | (주)케이비피 | Process for Preparing of Pullulan Employing By-product from Soybean Source Fermentation |
| KR100739022B1 (en) * | 2005-12-12 | 2007-07-12 | 동아대학교 산학협력단 | Method for continuous production of pullulan with feeding of the sucrose |
| US10568839B2 (en) | 2011-01-11 | 2020-02-25 | Capsugel Belgium Nv | Hard capsules |
| US11319566B2 (en) | 2017-04-14 | 2022-05-03 | Capsugel Belgium Nv | Process for making pullulan |
| US11576870B2 (en) | 2017-04-14 | 2023-02-14 | Capsugel Belgium Nv | Pullulan capsules |
| US11878079B2 (en) | 2017-04-14 | 2024-01-23 | Capsugel Belgium Nv | Pullulan capsules |
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