KR20010070763A - 3 omitted - Google Patents

Abstract

PURPOSE: A method for the treatment of third degree burns to human skin by extracting a burn-treating component from a vacuole in an eggplant cell and applying the obtained extract to the burn area is provided. Whereby, the extract has excellent effect on treating a burn. CONSTITUTION: Vac ga2001 as a burn treating component is obtained by extracting a vacuole in an eggplant cell by electrophoresis and distilling. The obtained extract is effective in treating third degree burns to human skin.

Description

Third-degree burn treatment using electrolysis of intracellular vacuoles of eggplants

The present invention relates to a third degree burn treatment using the electrolysis of intracellular vacuoles of eggplant.

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The present invention is a 3rd degree burn treatment using the electrolysis of intracellular vacuoles of eggplant

1. Efficacy of Eggplant

Eggplant cools the remaining heat on the skin.

2.plant plant cells

The cell solution contains various salts, sugars, organic acids, tannins, alkaloids, and pigments.

Among these, inorganic salts have the highest concentrations, and sugar and organic acids have the highest concentrations. In particular, the anthocyanin pigments are dissolved in the vacuoles of plant cells, which make the leaves and many flowers of maple trees unique.

It is much closer to the fluid and much less viscous than the cytoplasm.

However, due to the high concentration of lysate, it has a high osmotic value, and when it comes into contact with a low osmotic aqueous solution with a semipermeable vacuole or plasma membrane in between, the cell fluid has a high osmotic pressure, thereby maintaining a tensioned state due to swelling in the cell. Done.

On the contrary, when contacted with an aqueous solution with a higher osmotic value than the cell fluid, the water in the cell fluid escapes, causing the cells to lose swelling and cause wilting or protoplast separation.

Since the cell solution of a plant contains oxalic acid, acetic acid, malic acid, tartaric acid, citric acid, etc., it has a weak acidity of pH 5.0-6.0. Fruits contain anthocyanins, glucose and fructose. Plasma separation occurs because the plasma membrane is semipermeable and therefore cannot be seen in dead cells. Therefore, it is commonly used to determine the life and death of plant cells. In addition, the osmotic pressure of the cells can be determined by investigating the concentration of the external fluid in which the plasma separation just begins. The isolated plasma form is sometimes formed into an iron form or a concave form according to the cell type or the composition of the external fluid. From this, the adhesion between the cell wall and the plasma membrane and the surface tension of the plasma membrane can be estimated. . In addition, since animal cells do not have a cell wall, the whole cell contracts in the solution of high osmotic pressure, and the separation of the plasma is not observed. It is also called an operation potential. The ion composition inside and outside the cell is different due to the action of various ion pumps such as sodium and potassium present in the cell membrane. The difference in ion composition results in a negative potential (stopping potential) of 60 to 90 mV inside the cell membrane. When excitable cells such as nerves and muscles are excited, polarity inside and outside the cell membrane is changed, and the cells are translocated to 30-40 mV. This potential change is called the spike potential because it recovers in as fast as a few m / s and is distinguished from the late potential, a slow change seen in the recovery phase. The local current is generated by this potential change, and excitement is transmitted at a speed of 1 to 100 m / s. This potential change is as follows. Activity potentials are also seen in plant cells, such as sticky spatula and waterweed.

After plasma separation, the cells are deprived of moisture, and the electrolyte ions in the plasma become higher than the surrounding environment, and the osmotic pressure in the cytoplasm is increased. As a result, the relatively low concentration of the surrounding environment becomes a stock solution, resulting in partial protoplasma, in which water is reintroduced into the cell. Cells in which protoplasts have been isolated tend to increase cell permeability and reduce semipermeability to attract moisture, thereby helping to maintain protoplasma. In addition, faster protoplasma recovery occurs when cells that have undergone protoplast separation have been moved or moved to low concentration environments.

3. Third-degree Burn Treatment Using Electrolysis of Branched Cell Vacuoles

1. Fat analysis using Soxhlet extraction.

Low-boiling solvents such as petroleum ether and diethyl ether determine the fat extraction → drying → weight loss → fat content in the sample.

Analytical Procedure

1) Sample pretreatment: Drying, grinding, Na2SO4 dehydration, Cu (OH) 2 precipitation → Extraction → Solvent evaporation → Drying → Crude fat content until determined.

2) Weight after drying (g) × 100 / Weight of sample (g) Advantages and disadvantages of the Soxhlet method-No need for hydrolysis-Saves time-Uses low-cost equipment-Wide range of applications-Inappropriate when the protein or carbohydrate content is high- The result depends on the type of solvent and the extraction time.-Extracts other lipophilic substances together.-Phospholipids are partially extracted.-Large amount of solvent, difficult to apply.

3) B-811 Buchi Soxhlet Features-Simultaneous 4 sample extraction-Automatic extraction, washing and drying process-Storage up to 50 various operation programs-Minimizing solvent loss and odor with optimal heat transfer and perfect sealing system Prevents spills-Prevents oxidation of extracts by inert gas injection, quick drying-Equipped with two powerful heaters per container, high boiling point solvent and mixed solvents up to 150 ° C-PAK (hexan extract in soil) analysis, rapid extraction Time Acid Hydrolysis The Need for Hydrolysis Soxhlet Direct Extraction Does Not Extract All Fat Components-When Carbohydrates and Proteins Surround Fat-When Wrapped in Plant Cells or Starch-membrane-Floating Colloids Surround Fat Inexpensive (milk, cheese, cream)-dairy products, cereals, bread, eggs, fish, meat, tofu-acid digestion procedure-4M HCl added to decompose matrix components of sample-filtration It determines the subsequent extraction, drying fat content determined from the weight loss - more than fat separation - hot water washing - drying.

4) Acid Decomposition Features-Simultaneous Degradation of Four Samples-Fast and Efficient Filtration-Easy and Odorless Operation-Perfectly Harmonized with Buchi B-811 Extraction System New Fat Method Phospho-, di- and monoglycerides are three fatty acids It has lower calories than triglycerides. Each fat has a mixture of phospholipids. Phospholipids are classified as fats, but they are partially lost or difficult to extract completely. Extraction with solvents with high boiling point → acidification with KOH → acid Add solution → convert to fatty acid → determine fat content

5) Buchi B-815 / B-820 Composition and B-815 Extraction unit: Fat extraction and saponification B-820 Fat Determination unit: Determination of fat content based on fatty acid using gas chromatography technique Analyze and automate analysis to save analysis time, use non-polluting solvents, use methods recognized by AOAC, include phospholipids in final results, make simple, reliable, rapid analysis, and simple but rapid determination of butyric acid.

6) Operation Procedure

(One). Extracts fat

(2). KOH saponification.

(3). React with acid solution.

(Layer separation)

(4). Take supernatant and analyze using GC principle.

(5). Calculate total fat content based on fatty acids.

4.Electrophoresis of branch intracellular vacuoles

1) Definition and principle

Electrophoresis refers to the movement of charged materials in a fluidized medium under the influence of an electric field. Charged components include molecules such as proteins, cells, or charged particles. But what is covered here is limited to charged molecules (ions). Liquids or gases are used as the flowable medium, but liquids are mainly used in biological systems. What is covered in electrolysis is the mobility of molecules due to the influence of electric fields, not the reaction of electrodes in electrochemical techniques or the interaction of oppositely charged ions in ion exchange. The speed of charged molecules (particles) in the electric field is controlled by the coupling of retarding forces and the acceleration force (electric field). The charged molecule will thus reach and maintain its final velocity in a manner similar to the fall of any particle under the influence of gravity. The force F on a molecule is given by F = QE, where Q is the charge of the molecule and E is the electric field (potential difference across the electrode divided by the distance). The main stopping force Fs is due to friction and can be quantified by Stoke's equation as follows. Fs = 6 πγηυ where γ is the molecule (particle) radius, η is the viscosity of the liquid, and υ is the molecular velocity.

Other negative blocking forces are caused by other ions (counter ions and buffer ions) in the solution. Distortion occurs because ions of different mobility are distributed around the charged molecule to be analyzed.

Neglecting this distortion, charged molecules reach their final velocity when acceleration and stopping forces are equal. F = Fs or QE = 6 πγηυυ = QE / 6 πγη Molecules of different charges or sizes move at different rates, which is the basis of electrolysis. If there is an interaction between charged molecules and the supporting material, the situation becomes more complicated. Supporting materials are used to reduce other effects such as convection phenomena, which may form discontinuous bands and impede the movement of components. These effects are described in the techniques of electrolysis.

2. Effect of pH on Charge The effect of pH on the surface charge of molecules such as proteins depends on the number and type of groups that can be ionized. The effect of these acidic or basic groups is that the protein becomes net zero positive charge at its intrinsic pH (I p). At pH lower than the isoelectric point, the protein exhibits a net positive charge, and as the pH decreases, the net positive charge increases and the transport of protein increases in the electrolysis method. At pH higher than the isoelectric point, the protein will have a net negative charge and the mobility will increase with increasing pH. But in this case the direction is reversed. The effect of pH on molecular charge is also important for electrolysis as in ion exchange. The same is true for acids and bases, with the difference that the polarity of the charge does not change with the change of pH, only the magnitude of the charge changes. For example, at pH significantly lower than pI (at least 2 in pH units), the acid is essentially in ionized form and thus does not contribute to electrolysis. In practice, the interrelationship between electrolytic mobility and charge is more complicated. However, the concept of pI can be used effectively.

2) Electrolysis method on isoelectric connection

Using electrolysis over isoelectric connections, the charged molecules will continue to move as long as the electric field is applied, so the components must stop moving before they reach buffer reservoirs. Another method, isoelectric focusing (IEF), uses charged components (proteins) to focus at specific locations within the support material. A suitable mixture of ampholytes can be electrolyzed to achieve a pH gradient on the support material between the positive and negative electrodes. The most commonly used material is polyamino-polycarboxylic acid with a relative molecular mass of 300 to 600. As a result of the electrolysis, the anode becomes acidic and the cathode becomes basic, creating a pH gradient between the electrodes. When the sample is placed in such a system, the protein will move to a point where there is no net charge, i.e., pI in the same slope. If the protein leaves this point, it will be charged and move towards the anode or cathode to return to the point with zero charge (ie focusing into a very folded band). The resolution of the system is expressed as the minimum deviation between the pI of the required protein and the pI of the protein to be separated and depends on the degree of pH gradient. For preliminary analysis, use a wide range of slopes (low resolution) and use a narrower pH range for more precise analysis. The supporting material for suppressing convective currents in the IEF is concentrated sucrose solution. Recently, however, polyacrylamide or agarose gel is mainly used. Gels are stained after focusing and quantified using another gel technique. The original IEF, like most other electrophoretic techniques, was performed on short tube gels, but slab or thin film forms are increasingly common.

3) Extraction of liquid crystal of antigenic protein in branch intracellular vesicles

Antigen Protein A substance that attracts much attention in the production of heterologous protein using a plant as a production system is an antigen protein. When the antigenic protein is expressed in a plant, the plant itself can serve as a plant for vaccine production. In other words, the plant that produces the antigenic protein can be used as a material for the extraction of recombinant antigenic proteins for use in vaccines, and when the plant is used for food, it exhibits the effect of oral vaccines. Extract the liquid crystal of the antigenic protein.

4. The electrolysis process of intracellular vacuoles is as follows.

1) Matters to note

(1) The time required for experiment can be observed in 10 minutes when the power is set to 10V during electrolysis. If you increase the voltage as needed, it will run faster.

(2) When using U-clamps as clamps, it is better to fix them on both sides than on one side.

(3) When using U-tubes, use U-tubes with branches, otherwise the solution may overflow.

(2) preparations

10% aqueous cell solution, carbon rod, U-tube, switch, wire, power supply, stand, clamp

(3) Results

At the @ (+) pole, sodium ions are drawn toward the (+) pole, leaving electrons at the (+) pole to form a sodium vacuole.

Chlorine ions are drawn from the (-) pole toward the (-) pole, leaving electrons at the (+) pole to form chlorine vacuoles.

(4) Significance of results

As sodium and chlorine ions of intracellular vacuoles are decomposed, only pure vacuole components of eggplant are extracted, and the vacuoles are purified by distillation to extract an extract named vac ga200106, which is effective for third-degree burns.

As described above, according to the present invention, an extract named vac ga200106 is extracted, which is effective for the degree of burns, through a third degree burn treatment method using electrolysis of intracellular vacuoles of eggplant.

Claims (1)

3rd degree burn treatment using electrolysis of intracellular vacuoles of eggplant