KR20010003706A - Recycling Technology by Mixed Enzyme - Google Patents

Abstract

PURPOSE: A regeneration method of corrugated cardboard waste is developed to efficiently improve the strength and the dehydration property of the cardboard by using a suitable compound enzyme composition with a synergy effect. CONSTITUTION: A method for regenerating corrugated cardboard waste is characterized in that it uses a compound enzyme containing at least two or more of the enzymes selected from cellulase, hemicellulase and amylase based enzymes. The compound enzyme is contained at 0.01-0.1wt.% based on total dry weight of the corrugated cardboard waste. The compound enzyme activates at 40-70°C and under the pH values of 4.0-8.0 to increase the strength and the dehydrating load and minimize white water contamination to result in the improved quality of regenerated product and productivity.

Description

Recycling Method of Corrugated Cardboard by Combined Enzyme Treatment {Recycling Technology by Mixed Enzyme}

The present invention relates to a method for regenerating corrugated cardboard by combination enzyme treatment, and more particularly, to produce regenerated cardboard by regenerating from corrugated cardboard, that is, enzymes of different mechanisms, that is, cellulase-based enzymes, hemicellulase-based enzymes, and amylases. By properly formulating the system enzyme, it is possible to restore the flexibility of regenerated fiber by synergistic effect between enzymes, improve the strength, improve the dehydration load by starch used as fine fibers, foreign substances and adhesives, and minimize white water pollution. The present invention relates to a method for regenerating corrugated cardboard by compounding enzyme treatment that can be expected to improve productivity as well as to improve productivity.

As paper production and consumption are rapidly increased due to economic development and improved living standards, depletion of forest resources is a serious problem. As a result, many researches are being conducted in various angles in order to regenerate and use highland paper, which has been considered only as waste, and to reproduce high quality paper using highland as a raw material.

However, regenerated fiber deteriorates during its use as a paper and exfoliates through several mechanical treatments and drying processes, resulting in inferior swelling and flexibility and a large amount of microfibers. It also causes problems.

Therefore, conventional chemical enhancers and dehydration accelerators have been used for the purpose of improving the strength required for high quality paper and improving dehydration for smooth operation. However, with the growing awareness of environmental pollution, microbiological enzymes have been widely used in recent years to improve the strength and dehydration load of paper by using the hydrolysis characteristics of enzymes, biochemicals, without affecting the environment. As a result, research on this is being actively conducted.

In the microbiological enzyme application method, cellulase-based and hemicellulase-based enzymes generally attack the surface of the fiber, resulting in increased strength and piling effect due to fibrillation of the fiber Increasing has already been reported (JCPommier et al., Tappi Journal, 72 (6), 1989), and conventionally discloses a method of simply applying one of the enzymes described above for treatment.

However, in the case of corrugated cardboard, which is the lowest grade, only one type of enzyme is applied because of severe keratinization degree and severe dehydration load caused by a large amount of microfibers and foreign substances, especially corn starch, which is used as an adhesive when manufacturing cardboard. Existing enzyme application method is difficult to expect effective strength and dehydration improvement.

The inventors of the present invention, as a result of research efforts to solve the problems of the prior art, noticed that the enzyme-specific mechanism of action is different because the enzyme has a substrate specificity. Accordingly, an object of the present invention is to provide a new method for regenerating corrugated cardboard which can effectively improve dehydration and strength of regenerated pulp due to synergistic effect between two or three enzymes.

Figure 1 shows a triangular diagram for obtaining the optimal mixing ratio between the cellulase enzyme, hemicellulase enzyme and amylase enzyme.

The present invention relates to a method for regenerating corrugated cardboard, wherein a mixture of two or more enzymes selected from cellulase-based enzymes, hemicellulase-based enzymes and amylase-based enzymes is added at 0.01 to 0.1% by weight relative to the total weight of the corrugated cardboard. It is characterized by.

Referring to the present invention in more detail as follows.

The present invention is an improved method for treating a single enzyme, which is a mixture of two or more enzymes selected from enzymes of different mechanisms, that is, cellulase-based enzymes, hemicellulase-based enzymes, and amylase-based enzymes, more preferably an enzyme mixture of three components. It is a method of regenerating the corrugated cardboard by properly mixing according to the three-component design method.

First, each of these enzymes will be described in more detail.

The cellulase-based enzyme is an endo-cellulase-based enzyme produced from Trichoderma Longibrachiatum, and an endo-cellulase produced from such Trichoderma Longibrachiatum. -cellulase) enzymes exhibit optimal temperature and pH activity in the range of 40 to 75 ℃, pH 4.0 to 8.0.

In addition, the hemicellulase-based enzyme is a xylanase-based enzyme produced from Trichoderma Longibrachiatum, and a xylanase-based enzyme generated from Trichoderma Longibrachiatum. The enzyme exhibits optimum temperature and pH activity in the range of 40-70 ° C., pH 4.0-8.0.

Here, the cellulase-based and hemicellulase-based enzymes are known to increase the degree of freedom due to the strength and filing effect due to the fibrillation of the fiber by attacking the fiber surface as described above.

The amylase enzyme is an alpha-amylase enzyme produced from Bacillis licheniformis, and an alpha-amylase enzyme produced from Bacillis licheniformis. Shows the optimum temperature and pH activity in 40-90 degreeC and pH 4.0-8.0 range.

As described above, the corrugated cardboard, which is generally the lowest grade of recycled pulp, contains a large amount of foreign matter, and natural corn starch is used as an adhesive component in the core portion. As a result, when the starch component remains in the stock in the swollen state after dissociation during the process for recycling them, not only causes serious dehydration load but also increases the amount of contamination and waste of the process water.

Therefore, the present invention can also be used by applying only cellulase-based and hemicellulase-based enzymes, but by combining both enzymes with amylase-based enzymes at the same time, it acts as a dehydration inhibitor when regenerating cardboard. It will be able to have a decomposition ability for the starch.

Accordingly, the present invention has an optimum temperature in the range of 40 to 70 ° C. and optimum pH in the range of 40 to 70 ° C. when used in the form of a mixture of two or more selected from the above-described cellulase enzymes, hemicellulase enzymes and amylase enzymes. It is in the range of 8.0 so that papermaking can be carried out in neutral state without any pH adjustment, and it improves strength due to fibrillation of fibers, improves dehydration load caused by starch used as fine fibers, foreign substances and adhesives, and minimizes white water pollution. It can be expected to improve the quality of the corrugated cardboard, as well as to improve productivity.

On the other hand, the present invention was added to the above three kinds of enzymes at the dissociation of the corrugated cardboard to calculate the optimum enzyme compounding ratio with superior strength and dehydration effect according to the three-component design method, it will be described in detail.

First, the coefficients required for the physical properties calculated according to the proportion of the three enzymes of the enzyme of the three by substituting the measured value indicated in Table 1 burst strength and dehydration rate resulting blend ratio from 1 to 7 times in the following equation 1, b _1, ~ b ₇ was obtained. This means a coefficient corresponding to three vertices, three midpoints of each vertex, and one center point in the triangular diagram shown in FIG. 1. The physical properties in any compounding state were calculated using the coefficients of Equation 1 obtained by this method, and the results are shown as contour lines on triangular coordinates. In addition, the reliability of the assay was evaluated by comparing the actual measured values and calculated values according to the blending ratio of the three enzyme blends measured directly, that is, the blending ratio 8 to 10. The result is shown in a triangular diagram in Figure 1 attached.

In Equation 1;

and _{b 1 = Y 2, b 2} = Y 2 a, b ₃ = Y _{3 and, b 4 = 4Y 12 - 2} (Y 1 + Y 2) _{a, b 5 = 4Y 13 - 2} (Y 1 + Y 3 It is _{12 (Y 12 + Y 13 +} Y 23) +3 (Y 1 + Y 2 + Y 3) -) , _{and, b 6 = 4Y 23 - 2} (Y 2 + Y 3) and, b ₇ = 27Y _123.

As shown in the triangular diagram of FIG. 1, the most optimal compounding ratio was obtained. The method of adding 50:50 of the cellulase-based and amylase-based enzymes improved the strength of the corrugated support paper and accelerated the dehydration rate. Able to know. In addition, in the present invention, the amount of the enzyme mixture mixed at the compounding ratio as described above is added at 0.01 to 0.1% by weight based on the total weight of the total cardboard fat. If the amount of the enzyme mixture is not within the above range, There is a problem in that the significant effect of the invention cannot be obtained. On the other hand, when it is added in excess of the above range, the production cost is increased due to the use more than necessary. This is undesirable because there is a problem that degradation may occur and cause a decrease in strength.

As described above, by appropriately combining the enzymes of different mechanisms, such as cellulase-based enzymes, hemicellulase-based enzymes and amylase-based enzymes, by synergistic effect between the enzymes to restore the flexibility of the regenerated fibers to improve the strength, fine fibers In addition, it improves the dehydration load by starch used as foreign substances and adhesives, and can minimize white water pollution, so that the quality of the corrugated cardboard can be improved and productivity can be expected.

Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited by Examples.

Examples 1-7

Cellulase-based enzymes, hemicellulase-based enzymes and amylase-based enzymes were added to the total weight of the corrugated cardboard 200 g in the mixing ratios as shown in the following Table 2, respectively, and added 0.05% by weight, and the concentration was 3%, temperature 45 ° C, and pH 6.5. After dissociation for 15 minutes using a low concentration pulp under the aging, it was aged for 30 minutes. After concentrating the aged stock with 200 mesh wire, beating under the condition of rotating at 2700 rpm at a temperature of 20 ℃ with PFI-mill and following Tappi standard method T-205 om-88 It was hand papered with a basis weight of 150 g / m 2.

Comparative Example 1

In the same manner as in the above example, the enzyme was not added to 200 g of the total weight of the corrugated cardboard.

Comparative Example 2

In the same manner as in the above example, only 0.05% by weight of the cellulase-based enzyme was added as an enzyme with respect to 200 g of the total weight of the corrugated cardboard.

Comparative Example 3

In the same manner as in the above example, only 0.05% by weight of hemicellulase-based enzyme was added as an enzyme with respect to the total weight of the corrugated cardboard dry matter.

Comparative Example 4

In the same manner as in the above example, only 0.05% by weight of amylase-based enzyme was added as an enzyme with respect to the total weight of the corrugated cardboard total dry weight 200 g.

Experimental Example 1

Dehydration rate at papermaking was measured in Examples 1 to 7 and Comparative Examples 1 to 4, and the burst strength of papermaking was measured in Tappi standard method T-403 om-85, which is a standard measurement method of the American Pulp and Paper Association. It measured accordingly. In addition, the change of the microfiber content was analyzed using a Fiber Quality Analyzer (FQA, OpTest Equipment, Canada). The results are shown in Table 2 below.

In view of the above results, the strength and strength when the appropriate combination of two selected from cellulase-based enzymes, hemicellulase-based enzymes, amylase-based enzymes than the corrugated support paper prepared by simply adding one enzyme previously The dehydration improvement effect was found to be excellent. In addition, the overall fine content was reduced in the case of the enzyme treatment irrespective of the type and the mixing ratio of the enzyme than in the case of not treating the enzyme.

Experimental Example 2

Residual starch when no enzyme was treated (Comparative Example 1), when only 0.05% by weight of amylase-based enzyme was treated (Comparative Example 4) and when 50% and 50% of cellulase and amylase were formulated (Example 2). In order to measure the concentration of each pulp stock, each pulp paper was centrifuged, and then 0.1 ml of iodine indicator (KI + I2) prepared by taking 3 ml of the supernatant was absorbed using an ultraviolet spectrometer (UV spectrometer). absorbance) was measured. The results are shown in Table 3 below.

Looking at the measurement results, in the case of Comparative Example 4 it can be seen that the residual starch is significantly reduced. As a result, the amylase hydrolyzed the starch causing the dehydration load, thereby reducing the amount of residual starch, thereby improving the dehydration rate. Also, in the case of combining amylase with cellulase as in the case of Example 2, starch singer It was found that there was a decomposition effect.

As described above, according to the present invention, by appropriately combining at least two or more kinds of enzymes of different mechanisms, namely, cellulase-based enzymes, hemicellulase-based enzymes, and amylase-based enzymes, unlike conventional ones, The synergistic effect between enzymes improves the strength of regenerated fiber, improves strength, improves dehydration load by starch used as microfibers, foreign substances and adhesives, and minimizes white water contamination. Productivity can be expected to improve.

Claims (4)

In the method of regenerating the corrugated cardboard, the regeneration of the corrugated cardboard, characterized in that the mixture of two or more enzymes selected from cellulase-based enzymes, hemicellulase-based enzymes and amylase-based enzymes is added at 0.01 to 0.1% by weight based on the total weight of the corrugated cardboard. Way. The method of claim 1, wherein the cellulase-based enzyme is an endo-cellulase-based enzyme produced from Trichoderma Longibrachiatum. The method of claim 1, wherein the hemicellulase-based enzyme is a xylanase-based enzyme produced from Trichoderma Longibrachiatum. The method according to claim 1, wherein the amylase-based enzyme is an alpha-amylase (α-amylase) -based enzyme produced from Bacillis licheniformis.