KR20000061656A - Chinese medicine for cancer treatment - Google Patents

Abstract

PURPOSE: A Chinese medicinal composition for treating cancer is provided, which increases existing rate; decreases size of cancer; inhibits metastasis of cancer; which removes cancer cells by activating the immune system of living body not by attacking cancer cells directly. CONSTITUTION: A process for the preparation of Chinese medicinal composition for treating cancer comprises of: preparing herb medicines, and cutting finely; extracting the dried herb medicines using hot water; leaving the herb medicines in distilled water 1000ml at room temperature, boiling for 30minutes, and separating the extract and residue using filter sheath; adding distilled water 1000ml to the residue, boiling again for 30minutes, and filtering again; centrifuging the two extracts for 15minutes at 8000*g to get the supernatant; concentrating the extract under decompression, and freeze-drying to get the brown extract 13.4g. The composition contains 6-24wt.% of the AnJoongSan preparation, 25-100wt.% of the Bowhawhan preparation, 1-6wt.% of Sepia esculenta, Gallus domesticus, Scolopendra subspinipes, Rhus verniciflua, Scutellaria bardata, Anemarrhena asphodeloides each, and 0.25-1wt.% of Aucklandia lappa.

Description

Chinese medicine composition for cancer treatment {Chinese medicine for cancer treatment}

The present invention relates to a herbal composition for cancer treatment, comprising a combination of Anjungsan and Bohwahwan formulations, and adding herbal such as a checkpoint to a herbal composition for cancer treatment. It is about. The herbal composition of the present invention shows a more significant synergistic effect when mixed with Agaricus mushroom extract.

Cancer is the number one cause of death in the late 1990s and is on the rise. 30% of the 50s and 60s deaths in Korea are dying of cancer, and among the cancers, the most frequent deaths are gastric cancer, liver cancer and lung cancer. In Western medicine, early cancers can be treated with surgery, radiation, or chemotherapy, but late stage cancers or metastatic cancers require a life-long life, and side effects of radiation therapy and chemotherapy are also a major problem. It is becoming. Many cancer patients are suffering from side effects of radiation and chemotherapy, and are losing their lives. Herbal medicine can be applied to many cases that can not be repaired by Western medicine and can be said to have fewer side effects than Western medical therapy can improve the quality of life of cancer patients.

Attempts have been made to develop anticancer drugs in natural products with few side effects, but many patents have been filed and disclosed, but most plants (Korea Patent Publication No. 96-028914, etc.) and mushrooms (Korean Patent Publication No. 97-021287) It is about the development of anti-cancer drugs using the ingredients of Etc.), and it is very rare that the prescription is made based on the principle of oriental medicine.

Some published herbal anticancer drugs also lacked a test of scientific efficacy. (Korean Patent Publication No. 95-165, Korean Patent Publication No. 94-13527, Korean Patent Publication No. 97-73600).

In addition, Agaricus Blazei Murill has grown naturally in tropical mountains and has been successfully grown in Korea and Japan, and has been able to supply large quantities.Agaricus Blazei Murill can be supplied in large quantities. It contains a large amount of beneficial ingredients, and its anticancer effect is widely known. As a domestic patent for Agaricus mushroom, a case related to a health beverage manufactured by adding Agaricus mushroom extract together with a beverage adjuvant such as sweetener and citric acid glucose is disclosed (Korean Publication No. 98-75714).

Accordingly, an object of the present invention is to provide a herbal composition for treating cancer that can improve the quality of life of cancer patients without side effects by activating the biological immune system based on the principles of Chinese medicine and clinical symptoms to remove cancer.

Figure 1 compares the lungs of the control group mice with the herbal composition-administered group of the present invention in lung metastasis of B16 melanoma cancer cells.

An object of the present invention described above is 6-24 parts by weight of an intermediate acid formulation; 25-100 parts by weight of a boron ring preparation; And it is achieved by the herbal composition for cancer treatment which consists of 1-6 parts by weight of each of the mark, the inner gold, five holes, dried lacquer, ring lotus, hair, and 0.25-1 parts by weight of neck.

Hereinafter, this herbal composition is called kamiboa-tang (加味 保 我 湯).

In addition, when the agaricus mushroom extract is mixed with the herbal composition of the present invention, a synergistic effect is shown in cancer treatment. The composition of the agaricus mushroom is named Bogari-tang.

Although the name of stomach cancer does not appear in the literature of Chinese medicine, the symptoms of stomach cancer, gastric pain, deep heart attack, and severe symptoms are similar to those of gastric cancer. In summary, this leads to pain in the stomach and crisis and food buildup caused by the stomach's discomfort.

Kamiboa-tang was founded on the oriental medical stage and clinical symptoms of gastric cancer, and is listed in the Taepyeong Hyemin-gukbang National Defense Center (安 中 散). And Bohwahwan of 『Goldi Feeling (古 금)』, which consisted of a number of sweeteners considering the stage of gastric cancer.

The indication of Ahn Jung-san is based on the non-parallel, oxal water, and cold-air fluids, according to the Taepyeong Hyeminjeung National Defense. ), Static bloating, swelling of the thorax, celiac strait, wicked heart zone, cotton sulphate and dysfunction of the stomach It can be said that it is used for pain and general gastrointestinal symptoms caused by indigestion and instrumental depression. Its constituents are broiler, health, Goyanggang, Hyun-ho-ri-colored, rice, licorice, fennel

Bohwa-hwan is a food tablet according to Kogumgam-eui (今 醫 監).腐 厭食) and stool relief (大 便 不 調) are indications that treat the gastrointestinal damage caused by food and when used in the absence of food (飮 食 積滯) is a prescription that brings a communication effect. Can be. Its medicinal herbs are Baekchul, Dermis, Banja, Fuling, Singok, Sansa, Fellowship, Hyangbuja, Hubak, Nabojak, Golden, Jisil, Malt, Husband.

Therefore, the basic cubic principle of Kamiboa-tang (入 方 原理) focuses on improving the general symptoms of gastric cancer and restoring gastrointestinal function. , Gas, food, and phlegm, and summarized by the principle of treatment, Bowi, ego, news, and expectoration It can be called rough.

The specific control composition of kamiboa-tang is the combination of Ahnji-san and Bohwa-hwan, with the scents of Igiji-tong and Jojung-hwa in order to enhance the indication. It uses the strong antagonism of the antacid pain, and it uses the in-situ money, the detoxification, and the blue-green stalks, and the reddish odor. 건 (련 解), other detoxification, firefish, soybean smelting with small potency, sodam, lubricating thyme, and It is the effect of the neck with the effect of the cumulative year wall (治 積年 ?? 癖).

Gamiboa-tang was established based on the oriental medical stage and clinical symptoms of gastric cancer. Cytotoxicity studies on cancer cells and cancer survival prolongation experiments, cancer size reduction experiments, cancer metastasis suppression experiments and immunity were performed to confirm anticancer effects. Augmentation experiments were performed. Hereinafter, the present invention will be described in detail with reference to Examples. However, the present invention is not limited only to this embodiment.

Example 1

<Production of kamiboa bath>

The composition of Kamiboa-tang is shown in Table 1. Herbal medicine was purchased at Gyeongdong market, and after removing miscellaneous goods, it was cut and used for experiments. The well-dried herbs were chopped and extracted using hot water. 1000 ml of distilled water was added to herbal medicine, which was allowed to stand at room temperature for 24 hours, and then boiled for 30 minutes, and the extract and the medicinal herb were separated using filter paper. 1000 ml of distilled water was poured into the medicine, re-heated for 30 minutes, and filtered again to obtain an extract. The two extracts were combined and centrifuged at 8,000 × g for 15 minutes to extract only the supernatant. The supernatant was concentrated under reduced pressure in a 60 ° C. water bath using a rotary evaporator (Buchi RE 121, Switzerland) and lyophilized with a lyophilizer to obtain 13.4 g of a dark brown extract. This was dissolved in an appropriate concentration in phosphate buffered saline (administration) and administered to cancer cells and experimental animals.

[Table 1] Composition of kamiboa-tang

Example 2

<Cytotoxicity to Cancer Cell Lines>

Human gastric carcinoma (SNU-1), a Korean-derived gastric cancer cell line, was measured using an MTT assay (Moseman, T., J. Immunol. Methods, 65: 5510, 1983). The absorbance values of the experimental group finally obtained in this experiment represent the amount of MTT reduced by the enzyme of surviving cells, which is proportional to the number of living cells in each well. The concentration of the sample (IC ₅₀ ), which reduces the survival rate of cells, expressed by absorbance, to 50%, was used as an index of anticancer effect. As a result, Gamiboa-tang showed no direct cytotoxicity against gastric cancer cell line with IC ₅₀ value of 200µm / ml or more.

Example 3

〈Cancer survival extension experiment〉

ICR mice were divided into 10 groups in the control and experimental groups. Sarcoma-180 cancer cells were injected intraperitoneally with 2 × 10 ⁵ cells per mouse. (increase of life span: ILS) was obtained. The control group received the same volume of phosphate buffer solution.

T: Mean survival time of herbal group

C: mean survival time of control group

In this experiment, the control group was found to have died from 17 days to 32 days and the average life span was 21.0. In contrast, Gamiboa-tang started to die from 20 days and survived until 40 days, and the average survival time was 29.5, indicating an excellent anticancer effect with ILS (%) 140 (Table 2).

[Table 2] Effects of Gamiboa-tang on Cancer Survival

Example 4

<Solid Cancer Size Suppression Experiment>

B16 melanoma cancer cells were cultured and injected into the C57BL / 6 mice subcutaneously at 10 ⁶ cells / horse and 200 mg / Kg of gamiboa-tang was orally administered once a day for 15 days. The control group was orally administered an equal volume of phosphate buffer solution. After 15 days, rats were killed and necropsied to separate solid cancers and weighed with an electronic balance. Experimental results showed a statistically significant decrease in cancer size (Table 3).

[Table 3] Effect of Gamiboa-tang on Reduction of Solid Rock Size

a): mean ± standard deviation

*: Student's T test showed statistically significant decrease compared to control

(p <0.05).

Example 5

<Cancer metastasis suppression experiment>

The animals used for the experiment were 4 weeks old C57BL / 6 female mice at the Korea Experimental Animal Center, which were adapted for 4 weeks in the animal kennel and used for the experiment. 5 × 10 ⁵ B16 melanoma cells were suspended in 0.2 ml of phosphate buffer solution and injected into the caudal vein of C57BL / 6 mice, followed by oral administration once daily after 24 hours. After 14 days, the mouse was lethal and the lungs were removed. Then, the melanoma colony formed on the surface of the lung was observed under a dissecting microscope. As a result, as shown in FIG.

Example 6

〈Immune Boosting Effect Experiment〉

In the anti-cancer experiments using experimental animals, a study was conducted on the effects of kamiboa-tang on the biological immune system with high anti-cancer effects such as increased survival rate, reduced cancer size, and reduced cancer metastasis. Gamiboa-tang showed no direct effect on cancer cells with IC ₅₀ of 200 ㎍ / ml as a result of cancer cell cytotoxicity test. Therefore, the anti-cancer effect of gamiboa-tang is not a direct growth inhibitory effect on cancer cells but an immune function. The experiment was performed to confirm that the immune system is thought to be effective in removing cancer cells.

○ Effect on hemagglutinin

Antigens were stored at 4 ° C using cotton blood cells (SRBC, KOREA MEDIA OORP.), And only those within 1 week of storage were used. Gamiboa-tang and physiological saline were orally administered once a day for 21 days, and then immunized with 0.2 ml injection of cotton wool suspension of 5 × 10 ⁸ cell / ml in the control vein and the experimental group. The mouse was lightly anesthetized with ether, fixed on an anatomical plate, collected about 1 ml from the heart with a disposable syringe (syrintube, shown), left in the greenhouse for 1 hour, and the coagulated blood was stirred several times with a glass rod. The separator was centrifuged at 2,000 rpm for 30 minutes to obtain the supernatant serum. This serum was inactivated at 56 ° C. for 30 minutes and then used for the measurement of hemagglutination titer. To each well of a microtitration plate (Limbro chemical Co., Conn., USA), add 50 µl of 0.5% sheep red blood cell suspension to 25 µl of 2-fold dilution serum with phosphate buffered saline solution, and mix well. 37 ° C 5% C0 ₂ After standing for 18 hours in the incubator, the hemagglutination reaction was observed and read, and the maximum dilution factor of the serum causing the hemagglutination was measured at agglutination titer. Agglutination value of sheep blood cells between gamiboa-tang and control group was measured and calculated as log ₂ value. The control group showed 5.98 ± 2.24, and Gamiboa-tang 50 mg / kg group showed 6.72 ± 3.14. (Table 4).

[Table 4] Effect of Gamiboa-tang on Hemagglutinin Value

a): mean ± standard deviation

○ Measurement of rosette forming cell number

After the blood was collected, the mice were killed with cervical distal bone, and the abdomen was completely applied with alcohol to remove the spleen aseptically. Then, the tissues around the spleen were carefully removed and washed with cold RPMI medium. After finely crushing the prepared spleen and remove the debris and washed twice with RPMI medium. Thereafter, erythrocytes were destroyed with sterile distilled water, washed twice with 10 times Hank's balanced salt solution (HBSS) and once again with RPMI medium, followed by RPMI medium with 10% fetal bovine serum. The spleen lymphocytes were resuspended. The splenocyte suspension was adjusted to a concentration of 1 × 10 ⁷ cells / ml and the surface cell suspension, adjusted to a concentration of 3 × 10 ⁸ cells / ml, by 0.5ml each, and centrifuged at 980 rpm for 5 minutes. After standing at 4 ° C. for 30 minutes, the cells were resuspended with 1 ml of Hank's salt solution, and then the cell suspension was dropped on a hemocytometer (American 0ptica Buffalo, NY, USA) and examined at 450 magnification. The number of 10 ³ rosette-forming cells per 10 ⁶ splenocytes was determined as a rosette forming cell in which 4 or more sheep cells were attached to splenocytes.

As a result, the control group was 25.03 ± 2.58, while the 50 mg / kg administration group was 49.64 ± 5.72, nearly double the increase compared to the control group, and the significance of P <0.05 was also recognized (Table 5).

Table 5 Effect of Gamiboa-tang Administration on Rosette-forming Cell Number

a): mean ± standard deviation

*: Student T test showed statistically significant increase compared to control

(p <0.05).

○ Lymphocyte proliferation level measurement

Adjust the suspended spleen lymphocytes to a concentration of 1 × 10 ⁶ cells / ml by the above method, and then add Concanavalin-A (Sigma, USA), a T lymphocyte mitosis inducer, at a concentration of 10 μl / ml, and 96 100 μl per well was dispensed into a well culture vessel, and then cultured in a 37 ° C. 5% carbon dioxide incubator for 72 hours. [ ³ H] -thymidine (New England Nuclear, Boston MA, USA) was added followed by further incubation for 18 hours. Thereafter, the cells were collected on a glass fiber filter using an automatic cell collector (SKATRON, Skatron instrument, Norways), dried in a greenhouse, and placed in a radioactive measuring container and dissolved in a 5 ml measuring cocktail solution (5 g PPO, 250 mg P0P0P in 1 L of toluene). After dissolution, the amount of [ ³ H] -thymidine incorporated during DNA synthesis in a β-ray isotope detector (Beckman, LS 3801, USA) was measured by counter per minute (cpm). As a result, the control group was 142.76 ± 24.88 cpm, whereas the Kamigaatang 50 mg / kg administration group was increased to 896.48 ± 54.21 cpm and the significance of P <0.05 was recognized (Table 6).

[Table 6] Effect of Gamiboa-tang on Lymphocyte Proliferation

a): mean ± standard deviation

*: Student T test showed statistically significant increase compared to control

(p <0.05).

○ Effects on Macrophage Activity by Carbon Clearance

Macrophage activity was measured by injecting 16 mg of carbon into the vein of the mouse and collecting 25 μl of blood from the orbit at 1 minute and 5 minutes with a micropipette, and then hemolyzed in 2 ml of 0.1% Na ₂ CO _3. The spectrophotometer (Spectrophotometer, U-2000, Hitachi, Japan) was used to measure the carbon concentration in the microvascular at 675 nm wavelength. The index K was calculated by the following formula.

C ₁ : carbon concentration in sample blood at time T ₁

C ₂ : carbon concentration in sample blood at time T ₂

T ₁ : initial blood collection time

T ₂ : last blood collection time

In order to compare the macrophage activity between the experimental group and the control group, the carbon removal rate was measured by injecting carbon into the vein of the mouse. Increased to 0.00049, the significance of P <0.01 compared to the control was recognized (Table 7).

[Table 7] Effect of Gamiboa-tang on Macrophage Activity

a): mean ± standard deviation

*: Student T test showed statistically significant increase compared to control

(p <0.01).

Example 7

〈Synthesis effect of anti-cancer effect in bogari-tang (BKR) mixed with kamiboa-tang and agaricus>

Four-week-old ICR mice were intraperitoneally injected with 2 x 10 ⁵ Sarcoma-180 cancer cells, and the samples were orally administered once a day for 15 days. Percentage of live mice was observed 30 days after cancer cell injection. As a result, the control group showed a survival rate of 0%, whereas the Gamiboa-tang group showed 10% survival rate and the agaricus group 20% survival rate. When treated with kamiboa-tang and agaricus-based bogari-tang, 50% survival rate was observed, indicating a strong anticancer effect (Table 8).

[Table 8] Antitumor Effect of Bogari-tang (BKR) Mixed with Kamiboa-tang and Agaricus

As can be seen from the above, Gamiboa-tang, which consists of 28 herbal medicines, had no direct growth inhibitory effect on cancer cells, but activated bioimmune functions to increase cancer survival rate, decrease cancer size, and inhibit cancer metastasis. Indicated. Compared to conventional cancer treatments such as radiation therapy and chemotherapy, the side effects of bone marrow and immune system damage have been shown. It is a cancer drug. Also, when mixed with agaricus mushroom, which is widely known as an anticancer effect, gamiboa-tang increased the anti-cancer effect of agaricus mushroom. You can expect the effect.

Claims (2)

6-24 parts by weight of the eye acid preparation; 25-100 parts by weight of a ring-shaped formulation; And herbal compositions for cancer treatment, each consisting of 1-6 parts by weight of shedding point, gynecological intake, five-hole, dried lacquer, jinryum, jimo and 0.25-1 parts by weight The herbal composition for treating cancer according to claim 1, further comprising 35-150 parts by weight of agaricus mushroom.