KR20000005403A - Lycopersicon pimpinellifolium as a source of resistance to the plant pathogen phytophthora infestans - Google Patents

Abstract

PURPOSE: Tomato plants(Lycopersicon esculentum) and Lycopersicon pimpinellifolium plants is provided to be resistant to the tomato strain of P. infestans races 0 and 1. CONSTITUTION: After crossing an Lycopersicon pimpinellifolium plants discovered to contain a new allele(s) which confers resistance to P. infestans races 0 and 1 with an Lycopersicon esculentum, the seed is collected and regenerated into plants. The resulting plants are evaluated for resistance to the tomato strain of P. infestans races 0 and 1 and plants demonstrating resistance are identified and selected. These selected resistant plats are backcrossed with other Lycopersicon esculentum lines displaying desirable phenotypes to obtain commercially acceptable varieties which are resistant to the tomato strain of P. infestans races 0 and 1. The plants can also be produced by protoplast fusion.

Description

Lycoppericcon pimfinellifolium as a Source of Resistance to the Plant Pathogen Phytophthora infestans

Since the 1st century, late blight of tomato and potato plants has been of interest to plant breeders. Plague is common on cold, wet climates and can devastate lands during the wet season. Plague is a global problem, but it is particularly prevalent in the eastern United States, Mexico and the Far East.

The late blight is caused by a fungus called Phytophthora infestans ("P. infestans"). Phytophthora infestans can be found in plant debris, potato tubers and eggplant weeds. Fungal spores are typically scattered widely during windy and rainy days, but fungi can be scattered on infected plant tissue debris and fruits. If the spores settle on the leaves, stems and fruits of the tomato plant, the spores can quickly invade the plant tissues, disrupt the tissues and eventually kill the plants.

Currently, there are two physiological varieties of tomatoes described as blood infestans. First is blood infestans 0, which is pathogenic to commercial strains that do not have a resistance gene. The second is P. infestans 1, which is pathogenic to commercial strains with Ph ₁ resistance genes.

In 1952, resistance to blood infestans was reported in Lycopersicon Pimpinellifolium, a type of wild tomato. This resistance was found to be conferred by one dominant gene (Ph ₁ , formerly known as TR ₁ ) that provided resistance to the tomato bacterium P. infestans 0. See Gallegly, ME, "Resistance to the Late Blight Fungus in Tomato", (1960) Campbell Plant Science Seminar, p. 116. In addition, multigene resistance was found in tomato varieties West Virginia 700 (P1204996). This resistance is not complete and confers only resistance to blood infestans 0 and 1. When the experimental conditions favored the development of P. infestans 1, these resistant plants developed a serious disease.

Lycopersicon esculentum, a cultivated tomato, is one of the most important plant crops in the United States and the world, and millions of tonnes are produced annually in the United States alone. The commercial importance of these crops required constant efforts to improve the cultivars. Thus, there is a need for cultivated tomato varieties that exhibit disease resistance to blood infestans 1.

It is an object of the present invention to disclose a novel lycopperic pimplepinfolium strain that can be used as a resistance against the tomato bacterium P. infestans 1. It is another object of the present invention to provide a lycopperic cone esculentum plant that is resistant to the tomato bacteria P. infestans 0 and 1, and maintains resistance to P. infestans 1 even when the disease pressure is high. To provide.

Summary of the Invention

The present invention includes a method for producing a tomato plant (lycopericone esculentum) resistant to the tomato bacterium pi infestans 0 and 1. This plant is produced by hybridizing lycoppericone pimfinellifoliium plants and lycoppericone esculentum which have been found to contain novel allele (s) that confer resistance to blood infestans 0 and 1. After hybridization, seeds are collected and regenerated into plants. The obtained plants were evaluated for resistance to tomato bacteria pi infestans 0 and 1. Identify and select resistant plants. Selected resistant plants are then hybridized with other lycopperic cone esculentum varieties that exhibit the desired phenotype to obtain commercially acceptable strains resistant to the tomato strains P. infestans 0 and 1. This method can also be used to produce seeds that produce tomato plants (lycopericone esculentum) that are resistant to the tomato bacteria P. infestans 0 and 1.

It was found to have novel resistance to P. infestans 0 and 1, and the selected lycopperic pimfinellifolium used for hybridization with L. esculentum was then designated LA 2533. This is also called the Hope 84 by the inventors.

Plants of the invention can also be produced by protoplast fusion. In order to produce plants by protoplast fusion, protoplasts of lycoppericcon pimfinellifoliium plants resistant to the tomato bacterium pi infestans 0 and 1 and protoplasts of lycoppericcone esculentum are obtained. The protoplasts are then fused using standard protoplast fusion procedures well known in the art. As a result, allogeneic cells are obtained and regenerated into plants evaluated to be resistant to the tomato bacterium P. infestans 0 and 1. Identify and select resistant plants.

Lycopericon esculentum plants produced according to the method of the present invention are resistant to the tomato bacteria P. infestans 0 and 1, and tolerate to P. infestans 1 even in the field with high pressure of disease.

The present invention also includes tomato plants containing allele (s) conferring resistance to the tomato bacterium pi infestans 0 and 1 and seeds produced by the tomato plants.

The present invention relates to the production of tomato plants (lycopericone esculentum) resistant to the tomato bacterium P. infestans. If a plant shows no symptoms of disease when exposed to the tomato bacterium P. infestans, or a significantly reduced symptom than a less resistant plant, the plant is said to be disease resistant. The plant of the present invention is new and novel because it is resistant to the tomato bacterium pi infestans 0 and 1.

The inventors of the present invention have found a lycoppericcon pimpinellifoliium plant containing allele (s) conferring resistance to the tomato bacterium pi infestans 0 and 1. This lycoppericum pimfinellifolium plant can be used to produce tomato plants (lycopericone esculentum) that are resistant to the tomato bacterium P. infestans 0 and 1. For example, the lycoppericum pimpinifolium plants, designated LA 2533, were used to produce the plants of the present invention. The inventors also called the lycoppericicon pimfinellifolium plant, designated LA 2533, as hop 84. Seeds from LA 2533 (Hoff 84) were deposited with the American Type Culture Collection (ATCC), 12301 Parkron Drive 12301, Maryland, in accordance with the provisions of the Budapest Treaty. Seeds were deposited on December 14, 1995 and were assigned ATCC accession no. 97382. In addition, LA 2533 (Hope 84) is available upon request from the Rick Center of the University of California-Davis, Davis, CA.

We found that the LA 2533 plant was resistant to the tomato bacterium P. infestans 0 and 1. Prior to this discovery, resistance to blood infestans 0 and 1 in plants named LA 2533 was unknown. LycoPericicon piffininifolium plants, designated LA 2533, contain allele (s) conferring resistance to the tomato bacterium P. infestans 0 and 1. However, one of ordinary skill in the art will appreciate that lycopericone pimpinifolium, which contains the allele (s) and is resistant to the tomato bacterium P. infestans 0 and 1, may be used in the present invention.

The plants of the present invention can be produced by traditional breeding techniques. For example, one may use seeds of the lycophericone pimpinifolium plant designated LA 2533. Seeds of LA2533 are planted in greenhouses or outdoors. The obtained plant is exposed to the tomato bacteria pi infestans 0 and 1. After sufficient disease pressure, the plants with the best resistance are selected. Resistance to P. infestans 0 and 1 can be determined in two ways. One way of determining resistance to blood infestans involves the use of the so-called "greenhouse screen" method. In greenhouse screening, tomato seedlings are grown until five sesame leaves emerge. At this time, the plants are sprayed with a suspension of blood infestans 1 spore sac (concentration is 10,000 spore sacs per ml of solution). After inoculation, the plants are placed in a chamber at 100% humidity for 24 hours. The plant is then removed from the humidity chamber and transferred to a greenhouse bench for disease to occur. Within 7 days resistant plants are identified and selected. The plant may then be secondary sprayed with a suspension of blood infestans 0 in the same manner. However, plants do not need to be tested for resistance to P. infestans 0 multiple times, as those skilled in the art generally know that tomato plants that are resistant to P. infestans 1 are necessarily resistant to P. infestans 0. Because.

The second method of determining resistance to infestans 0 and 1 is the field screen method. Field screening involves transplanting seedlings into field strips where climatic conditions are beneficial for natural infections and disease outbreaks. After the disease occurs, almost when the plants begin to bear fruit, the resistant plants are identified and selected.

The inventors of the present invention devised a disease assessment grade to assess whether the plants produced by the method of the present invention are resistant or weakly resistant to blood infestans 0 and 1. Disease ratings are based on the numbers 1 to 5, which are more specifically described as follows: 1 corresponds to no or small lesion on the leaf; 2 corresponds to no or small lesions on the leaves, enlarged leaf lesions, and small lesions on the leaf spigot (diameter 2 mm); 3 indicates no or small lesions on the leaves, enlarged leaf lesions, small lesions on the leaf pacifier (diameter 2 mm), and enlarged lesions on the pacifiers; 4, no or small in leaf, enlarged leaf lesion, small leaflet (2 mm diameter) on leaf tip, focal enlargement on leaf tip, small lesion (2 mm diameter) on stem; 5 has no or small lesions on the leaves, enlarged leaf lesions, small lesions on the pacifier (2 mm in diameter), enlarged lesions on the pacifiers, small lesions (2 mm on the stem), and enlarged lesions on stems or dead plants It is equivalent to. Plants rated at disease ratings less than 3 are considered resistant, and plants rated at disease rating 3 or higher are considered to be less resistant.

Traditionally, most plant breeders do not evaluate, identify, and screen tomatoes that are resistant to blood infestans. Most breeders do not study blood infestans because there is no concern that the fungus will kill the entire crop. Instead, most breeders control blood infestans by spraying fungicides to prevent the fungus from invading crops.

The pollen of the plant with the highest resistance is placed in the pistil of another plant to hybridize the plant to the commercial tomato plant (El Esculentum). After the tomato fruit opens, collect seeds. Seeds are grown into plants and assessed for disease resistance. Resistant plants may be selected and hybridized with commercial tomato plants with desired phenotypic characteristics such as plant type (inflorescence finite or gunshot), fruit size, morphology, aroma, color and long shelf life. The hybridization may be continued until a strain with the desired phenotypic characteristics and resistant to the tomato strains P. infestans 0 and 1 is obtained. The obtained plant is stable against P. infestans 1 even in the open air with high disease pressure.

It is interesting to note that Lycopethic Esculentum is abolished fertility. Therefore, there is a high possibility that cross-pollination occurs between lycophericone pimfinellifolium and lycoppericone esculentum without human technical intervention in the wild. In addition, there is no possibility that identification, selection and backcrossing of allele (s) conferring resistance to blood infestans 0 and 1 will occur in nature.

Protoplast fusions can be used to produce the plants of the invention. For example, a first protoplast is obtained from lycophericone pimpinifolium, designated LA 2533, and a second protoplast is obtained from lycophericone esculentum. The protoplasts are then fused using traditional protoplast fusion procedures well known in the art.

For example, protoplast fusion can be achieved using polyethylene glycol (PEG), which causes a deadlock in the presence of a fusion buffer (ie, a high pH solution that allows cell membranes to fuse). Such somatic hybridization can also be performed under conditions for producing interspecies hybrids or variants thereof, as disclosed in Sundberg et al., Plant Science, 43, (1986) 155, which is incorporated herein by reference. However, those skilled in the art will appreciate that protoplast fusion can be performed in a manner other than the use of polyethylene glycol (PEG). For example, protoplasts are described in Koop et al., “Electric Field-Induced Fusion and Cell Reconstruction with Preselected Single Protoplasts and Subprotoplasts of Higher Plants,” Electroporation and Electrofusion in Cell Biology, Numan et al. Fusing using the field inductive fusion technique described in Edit, pp. 355-365. Protoplast fusion is also described in Hauptmann et al., “Carrot × Tobacco Somatic Cell Hybrids Selected by Amino Acid Analog Resistance Complementation,” 6th International Protoplast Symposium, Basel, Aug. 12, 1983. 1-16 days], using dextran and polyvinyl alcohol.

In the following, examples of the present invention are provided, which are for illustrative purposes and do not limit the present invention.

Example 1

Several different plants were evaluated for resistance to blood infestans. The following seeds were obtained: El Pimfinellipoliium, designated LA 373, El Esculentum Bar, designated LA 1338. L. esculentum var.cerasiforme, El Pimfinellipoliium, labeled LA 1375, L.hirsutum, labeled LA 2650, and El Pimfinellipoli, labeled LA 2533 (Hoff 84). cellar. All of these seeds were obtained from Rick Center, University of California-David, Davis, CA. Hope 33 is a tomato variety that is resistant to pi infestans 0 and 1. Celebrity and Fandango are commercial varieties that show no resistance to blood infestans.

Seeds were sown in a greenhouse. Tomato seeds were sown in soil (50% moss: 50% sand), placed on benches in the greenhouse (75 ° F. weekly and 70 ° F. at night) and watered as needed. When five seedlings of tomato seedlings were inoculated, they were inoculated with blood infestans 1. Seedlings were sprayed at a concentration of 10,000 spore sacs per ml of solution until all of the suspension of blood infestans spore sacs was depleted. After inoculation, the plants were placed in a chamber at 100% humidity for 24 hours. The plant was then taken out to cause disease. Approximately 7 days after inoculation, resistant plants were identified and selected using the following disease classes. These same varieties described above were evaluated for P. infestans O in the same manner.

Disease grade

1: no leaves on the leaves or small

2: no leaf on the leaf, small leaf lesion enlarged, small leaf lesion (2 mm diameter) on leaf tip

3: leaf no lesion or small, leaf lesion enlarged, leaf lesion small lesion (diameter 2mm), leaf lesion enlarged

4: no or small on the leaf, enlarged leaf lesion, small on the pacifier (diameter 2mm), enlarged on the pacifier, small on the stem (2mm diameter)

5: no or small lesions on the leaves, enlarged leaf lesions, small lesions (2 mm in diameter) on leafstalks, enlarged lesions on scapes, small lesions (2 mm in diameter) on stems, enlarged lesions on stems or dead plants It was.

Disease grading results are shown in Table 1 below:

kind Disease grade Number of plants Infestans 1 Infestans 0 Hope 33 3.0 1.0 9 Celebrity 5.0 2.3 9 Judgment 5.0 2.3 9 LA 373 L. Pimfinellipoliium 5.0 3.0 6 LA 1338 L. Esculentum Bar. Cerashiform 5.0 1.3 6 LA 1375 L. Pimfinellipoliium 4.3 1.0 6 LA 2650 L. Hirtsumum f.Thippikum 4.3 1.2 6 LA 2533 L. Pimfinellipoliium 1.3 1.0 6

The table above shows that the elpimfinellifoliium plants, represented by LA 2533, have a high level of resistance to piinfestans 0 and 1, while the elpimfinellifoliium strains, represented by LA 373 and 1375, have a high level of resistance. It does not show.

Example 2

A. El Pimfinellifolium plants, designated LA 2533 and also referred to as Hope 84, were hybridized with tomato (L. Esculentum) plants, designated Fla 7065. The plant Fla 7065 is a fresh, allogeneic breeding fruit with a finite, fruity, even green color, obtained by Dr. Jay Scott's breeding program at the University of Florida, Florida, USA. . Seeds obtained from these hybrids (F1) were sown in soil, grown in greenhouses and inoculated with blood infestans 1 as described in Example 1. When using the same disease class as described in Example 1, all seeds obtained above had a grade of 1 or 2, demonstrating that they are resistant to blood infestans 0 and 1. This suggests that the gene (s) involved in resistance are dominantly inherited.

Example 3

The F1 plants described in Example 2 were self-pollinated. Seeds from this F2 were sown in soil and grown in greenhouses. After several weeks, the seeds were transferred to the field and water was supplied by dropping irrigation and grown to ripen. Before flowering, it was cut, transferred to soil and grown in a greenhouse. Two weeks after cleavage, blood infestans 1 (concentration: 10,000 spore sacs per ml) was inoculated and placed in a room with 100% humidity for 24 hours. After 24 hours, the plants were removed and the disease progressed. After 14 days the cuts were graded 1-5. The disease grade used here is as follows:

1: no leaves on the leaves or small

2: no leaf on the leaf, small leaf lesion enlarged, small leaf lesion (2 mm diameter) on leaf tip

3: leaf no lesion or small, leaf lesion enlarged, leaf lesion small lesion (diameter 2mm), leaf lesion enlarged

4: no or small on the leaf, enlarged leaf lesion, small on the pacifier (diameter 2mm), enlarged on the pacifier, small on the stem (2mm diameter)

5: no or small lesions on the leaves, enlarged leaf lesions, small lesions (2 mm in diameter) on leafstalks, enlarged lesions on scapes, small lesions (2 mm in diameter) on stems, enlarged lesions on stems or dead plants It was.

The grades for the F2 plants are shown in Tables 2a to 2d below.

Breeds with a disease grade of 2.9 or lower were considered resistant, while varieties with a disease grade of 3.0 or higher were considered to be weak (71 varieties were resistant and 24 varieties were weak). The data in Table 2 shows that the ratio of resistant varieties: less resistant varieties is 3: 1, which is expected when the resistant locus is dominant. However, many varieties have disease grades 2.0 to 2.8. The inventors believe that these varieties may be heterozygous. If this is true, this suggests that resistance is partially dominant inheritance when homozygous plants have a higher level of resistance than heterozygous plants.

Example 4

The F2 plants described in Example 3 were self-pollinated. Seeds from this F3 were sown in soil and grown in greenhouses. After several weeks, the seeds were transferred to fields with weather conditions suitable to cause natural infection and disease progression of blood infestans 1. Serious disease symptoms became apparent when the less resistant control plants (celebrities) died. The disease was graded from 1 to 5 when the plants began to bear fruit. The disease grade used here is as follows:

1: no leaves on the leaves or small

2: no leaf on the leaf, small leaf lesion enlarged, small leaf lesion (2 mm diameter) on leaf tip

3: leaf no lesion or small, leaf lesion enlarged, leaf lesion small lesion (diameter 2mm), leaf lesion enlarged

4: no or small on the leaf, enlarged leaf lesion, small on the pacifier (diameter 2mm), enlarged on the pacifier, small on the stem (2mm diameter)

5: no or small lesions on the leaves, enlarged leaf lesions, small lesions (2 mm in diameter) on leafstalks, enlarged lesions on scapes, small lesions (2 mm in diameter) on stems, enlarged lesions on stems or dead plants It was.

Disease graded results are shown in Tables 3A and 3B below.

The results show the results from the F2 plants described in Example 3. Plants with homozygous resistance identified in F2 plants (94FH98, 94FH99, 94FH114, 94FH120, 94FH121, 94FH148, 94FH162, 94FH170) and less resistant plants (94FH95, 94FH110, 94FH116, 94FH128, 94FH129, 94FH155, 94F188) Showed the same disease class in F3 plants. If resistance is conferred from numerous genes (multigenic complex), it will not be isolated in a 3: 1 ratio and homozygous resistant plants will not be identified as easily and quickly as demonstrated.

The invention described and claimed herein is not limited to the specific embodiments disclosed herein, because these embodiments illustrate several aspects of the invention. Any equivalent embodiment would fall within the scope of the present invention. Indeed, it will be apparent to those skilled in the art that various modifications may be made to the present invention from the foregoing description, in addition to those described herein. Such modifications are also considered to be within the scope of the appended claims.

Claims (21)

a. Providing a lycophericone pimfinellifolium plant that is resistant to the tomato bacterium Phytophthora infestans races 0 and 1; b. Hybridizing the Lycopersicon pimpinellifolium plant obtained in step a with the Lycopersicon esculentum plant; c. Collecting seeds from the hybrid plants obtained in step b; d. Regenerating the seed on a plant; e. Evaluating the d plant for resistance to phytophthora infestans 0 and 1; And f. Identifying and selecting plants resistant to phytophthora infestans 0 and 1 A method of producing a tomato plant having resistance to the tomato bacteria, phytophthora infestans 0 and 1 comprising. The method of claim 1, backcrossing the plant selected in step f with a lycopperic esculentum plant having the desired phenotypic trait; Harvesting seeds from the hybrid plants; Regenerating the seed to plants evaluated to be resistant to the tomato bacterium Phytophthora infestans 0 and 1; And identifying and selecting plants that are resistant to phytophthora infestans 0 and 1. The method of claim 1, How plants are evaluated using the following disease grade criteria from 1 to 5: 1: no leaves on the leaves or small 2: no leaf on the leaf, small leaf lesion enlarged, small leaf lesion (2 mm diameter) on leaf tip 3: leaf no lesion or small, leaf lesion enlarged, leaf lesion small lesion (diameter 2mm), leaf lesion enlarged 4: no or small on the leaf, enlarged leaf lesion, small on the pacifier (diameter 2mm), enlarged on the pacifier, small on the stem (2mm diameter) 5: no or small lesions on the leaves, enlarged leaf lesions, small lesions (2 mm in diameter) on leafstalks, enlarged lesions on scapes, small lesions (2 mm in diameter) on stems, enlarged lesions on stems or dead plants It was. The method of claim 1, The method of lycopperic pim finellifoliium plants is LA 2533. The method of claim 4, wherein The lycophericum pimpinifolium plant is LA 2533 with ATCC Accession No. 97382. a. Hybridizing the lycophericone pimpinifolium plant, designated LA 2533 with ATCC Accession No. 97382, with the lycophericon esculentum plant; b. Collecting seeds from the hybrid plants obtained in step a; c. Regenerating the seed on a plant; d. Evaluating the plant of step c for its phytoptophera infestans 0 and 1; And e. Identifying and selecting plants resistant to phytophthora infestans 0 and 1 A method of producing a tomato plant having resistance to the tomato bacteria, phytophthora infestans 0 and 1 comprising. The method of claim 5, hybridizing the plant selected in step e with a lycopperic esculentum plant having a desired phenotypic trait; Harvesting seeds from the hybrid plants; Regenerating the seed on a plant and evaluating resistance to the tomato strain Phytophthora infestans 0 and 1 to the plant; And identifying and selecting plants that are resistant to phytophthora infestans 0 and 1. The method of claim 6, How plants are evaluated using the following disease grade criteria from 1 to 5: 1: no leaves on the leaves or small 2: no leaf on the leaf, small leaf lesion enlarged, small leaf lesion (2 mm diameter) on leaf tip 3: leaf no lesion or small, leaf lesion enlarged, leaf lesion small lesion (diameter 2mm), leaf lesion enlarged 4: no or small on the leaf, enlarged leaf lesion, small on the pacifier (diameter 2mm), enlarged on the pacifier, small on the stem (2mm diameter) 5: no or small lesions on the leaves, enlarged leaf lesions, small lesions (2 mm in diameter) on leafstalks, enlarged lesions on scapes, small lesions (2 mm in diameter) on stems, enlarged lesions on stems or dead plants It was. a. Fusing the protoplasts from the lycoppericcon pimpinifolium plant resistant to the tomato bacterium phytoptotora infestans 0 and 1 with the protoplasts from the lycoppericcone esculentum plant; b. Regenerating the allogeneic cells thus obtained into lycoppercone esculentum plants that were evaluated to be resistant to tomato strains of Phytophthora infestans 0 and 1; c. Identifying and selecting plants resistant to phytophthora infestans 0 and 1 A method for producing a tomato plant having resistance to phytoptotora infestans 0 and 1, comprising. The method of claim 9, The lycophericone pimfinellipoliium is LA 2533. The method of claim 10, The lycophericum pimpinifolium plant is LA 2533 with ATCC Accession No. 97382. A tomato plant which is resistant to the phytophthora infestans 0 and 1, which is a tomato bacterium, produced by the method of claim 1, 6 or 9. Seed produced by the plant of claim 12. A tomato plant containing alleles resistant to the phytophthora infestans 0 and 1, which are tomato bacteria, prepared by the method according to claim 1, 6 or 9. Seed produced by the plant of claim 14. a. Providing a lycoppericcon pimpinelliporium plant resistant to the tomato strain Phytophthora infestans 0 and 1; b. Hybridizing the lycoppericcon pimfinellifolium plant obtained in step a with the lycoppericcone esculentum plant; c. Collecting seeds from the hybrid plants obtained in step b; d. Regenerating the seed on a plant; e. Evaluating the plant of step d for its resistance to phytophthora infestans 0 and 1; f. Identifying and selecting plants resistant to phytophthora infestans 0 and 1; g. f) hybridizing the plant of step f with a lycopericone esculentum plant having a desired phenotypic trait; And h. collecting seeds from g stage hybrid plants to produce tomato plants resistant to the tomato bacterium Phytophthora infestans 0 and 1 Produced by the method comprising, tomato plants having resistance to phytoptotora infestans 0 and 1. a. Fusing the protoplasts from the lycophericone pimpinelifolium plant resistant to the tomato bacterium phytoptotora infestans 0 and 1 and the protoplasts from the lycopericone esculentum plant; b. Regenerating the allogeneic cells thus obtained into lycopperic cone esculentum plants evaluated to be resistant to the tomato bacterium Phytophthora infestans 0 and 1; c. Identifying and selecting plants resistant to phytophthora infestans 0 and 1 Tomato plants produced by the method comprising a resistance to phytoptotora infestans 0 and 1. a. Providing a lycoppericcon pimpinelliporium plant resistant to the tomato strain Phytophthora infestans 0 and 1; b. hybridizing the lycoppericcon pimfinellifolium provided in step a with the lycoppericcone esculentum plant; And c. collecting seeds from the hybrid plants produced in step b to produce tomato plants resistant to the tomato bacteria Phytoptotora infestans 0 and 1 Method for producing a seed to produce a tomato plant resistant to the tomato bacterium Phytophthora infestans 0 and 1. a. Providing a lycoppericcon pimpinelliporium plant resistant to the tomato strain Phytophthora infestans 0 and 1; b. Hybridizing the lycoppericcon pimfinellifolium plant obtained in step a with the lycoppericcone esculentum plant; c. Collecting seeds from the hybrid plants obtained in step b; d. Regenerating the seed on a plant; e. Evaluating the d plant for resistance to phytophthora infestans 0 and 1; f. Identifying and selecting plants resistant to phytophthora infestans 0 and 1; g. f) hybridizing the plant of step f with a lycopericone esculentum plant having a desired phenotypic trait; And h. collecting seeds from g stage hybrid plants to produce tomato plants resistant to the tomato bacterium Phytophthora infestans 0 and 1 A method of producing a seed for producing a tomato plant resistant to the tomato bacteria phytophthora infestans 0 and 1 comprising. The method according to any one of claims 16 to 19, The method of lycopperic pim finellifoliium plants is LA 2533. The method of claim 20, The method of lycopperic pim finellifoliium plants is LA 2533 with ATCC accession number 97382.