KR19980081816A - Peripheral, sulfur-free, cheongsimwon composition containing no musk - Google Patents

Abstract

The present invention relates to a volatile sulphate cheongsimwon composition having a new prescription that does not contain musk and exhibits the same pharmacological effect as that of sulphate cheongsim. More specifically, the present invention is an active ingredient in powdered medicine 190-370, licorice 140-270, white ginseng 60-130, turmeric 70-130, Singok 70-130, soybean yellow soybean 40-100, cinnamon 40-100, glue 40- 100, Peony 30-90, Macmun-dong 30-90, Golden 30-90, Dongguk 30-90, Windproof 30-90, Baekchul 30-90, Shiho 30-70, Gilkyung 30-70, Pedestrian 30-70, Bokyeong 30- 70, Cheongung 30-70, Sulfur 10-20, Nutrient 20-50, Cerebrum 20-60, Whiteworm 15-45, Health 15-45, Camphor 10-30 and Seokchangpo 300-700, and Benzoin 20-60 as needed The present invention relates to a new sulfur-free blue cheongsimwon composition of the new formulation mixed with a weight ratio of.

Description

Peripheral, sulfur-free, cheongsimwon composition containing no musk

The present invention relates to a new formulation of the sulfur-free cheongsimwon composition (hereinafter referred to as the periphery of the sulfur sulfur cheongwon composition) of the new prescription. More specifically, the present invention relates to a composition of the periphery of the wet sulfur blue water reconstituted to exhibit the same pharmacological effect as the far-wool blue cheongwon by combining the camphor and quarry instead of the musk in the composition of the existing far-woong cheongsimwon.

Woohwang Cheongsimwon is a representative herbal medicine that has been used as a first-aid medicine for stroke, arteriosclerosis, hypertension, palpitations, dyspnea, mental anxiety, angina pectoris, acute paralysis, autonomic ataxia, and inability to die for hundreds of years.

However, the recent entry into force of the Washington Convention on the International Trade of Wild Animals has banned the international trade of musk, an important medicinal ingredient of whey, which makes it virtually impossible to use musk ingredients.

Musk is a musk gland secretion of the musk deer ( Moschus moschiferus L.) belonging to the family Cervidae, or other related animal males. It is used for consciousness disorders of the city, convulsions, strokes, mental disorders, inability to greet, impaired perception, mental anxiety, and cranial nerves. Therefore, if the musk component is not included in the whey, the natural pharmacological effect expected from the whey will be lost or reduced.

As such, various studies are being conducted in preparation for the case where musk is removed from the womb cheongsimwon. These studies are largely progressed in two ways: by replacing musk with a new herbal medicine that can replace musk's pharmacological action, and by replacing other medicines. However, if the musk is replaced with other medicines not included in the original Wowang Cheong Shim's original prescription, it is strict to decide which herbal medicine to use because it may cause serious changes in Whwang Cheong Shim's original pharmacological action. It must be determined through experimentation.

Accordingly, the present inventors have found various herbal medicines in order to find a composition that can exhibit the same pharmacological effect as the existing sulfur sulfur cheongwon while preventing the change in pharmacological action that may occur when the musk is replaced with other medicines not included in the original prescription of the sulfur sulfur cheongsimwon. Intensive studies were conducted by combining the agents. As a result, the composition containing the musk from the ingredients contained in the existing prescription of the Whangcheongsimsimwon, instead of the camphor and stone Changpo, which has a similar pharmacological effect to the musk, does not change any unwanted pharmacological effect, It was confirmed that the same pharmacological effect can be achieved to complete the present invention.

Figure 1 is a graph showing the effect of the sulfur-free blue heart composition of the present invention on the blood pressure in the spontaneous hypertension rat (SHR) compared to the effect of the sulfur-containing green heart composition (composition) containing musk [-◆-control (physiological saline), -●-positive control group (captopril),-▲-comparative composition pretreatment group,-■-test composition pretreatment group].

FIG. 2 is a graph showing the effect of the whey of blue milk composition of the present invention on the coagulation time of the mouse compared with the effect of the bovine sulfur blue heart composition (composition) containing musk.

That is, in the present invention, instead of excluding musk from the existing pharmaceutical ingredients of far sulfur cheongsimwon composition, by mixing the camphor and stone spear as an alternative herbal ingredient for musk, the same pharmacology as the far sulphur cheongwon containing musk as a whole without containing musk. The constipation sulfur sulfur cheongsimwon composition which produces the effect was comprised.

Camphor and Seokchangpo used as an alternative herbal medicine for musk in the present invention as a herbal medicine having a similar medicinal action to musk, instead of excluding musk in the composition of far-woong cheongsimwon caused by the exclusion of musk by combining them in a specific ratio It is possible to almost completely rule out changes in the pharmacological effects inherent in the WWW Cheongsimwon.

Other components of the far-blue sulfur cheongsimwon can maintain the content of the existing ingredients, but the composition ratio can be changed slightly depending on the desired therapeutic effect or individual differences, and the sulfur sulfur cheongwon composition having such a modified composition ratio is also included in the scope of the present invention. .

Specifically, the periphery sulfur sulfur cheongsimwon composition falling within the scope of the present invention has the following components and composition weight ratio: acid powder 190-370, licorice 140-270, white ginseng 60-130, turmeric 70-130, Singok 70-130, soybean Kwon 40-40, Cinnamon 40-100, Glue 40-100, Peony 30-90, McMoon-Dong 30-90, Golden 30-90, Angelica 30-90, Windproof 30-90, Baekchul 30-90, Seaho 30-70, Gil-kyung 30-70, Pedestrian 30-70, Bokryeong 30-70, Cheongung 30-70, Wyrm 10-20, Nutrition angle 20-50, Long-brain 20-60, Whiteworm 15-45, Health 15-45, Camphor 10-30 and Seokchangpo 300-700.

In addition, the composition of the present invention blended in the above ratio may be blended with a benzoate in a weight ratio of 20 to 60, as necessary. Benzoin is a herb that has a similar effect to musk, and by combining it, the pharmacological effect of whey powder can be obtained more completely.

The mutated milk sulfur cheongwon circle composition of the present invention can be prepared in a pill formulation, such as pills or suspensions or gels according to conventional methods in the pharmaceutical field using a pharmaceutically acceptable carrier as needed.

Hereinafter, the present invention will be described in detail based on the preparation and experimental examples.

Preparation Example 1: Suspension Composition

Caesar 14.1g, Licorice 10.1g, White Ginseng 4.85g, Sulfur 5.0g, New Song 5.0g, Soybean Yellow Soybean 3.5g, Cinnamon 3.5g, Peony 3.0g, Macmundong 3.0g, Golden 3.0g, Angelica 3.0g, Windproof 3.0g, Baekchul Approximately 630 ml of water is added to 106.3 g of raw medicines containing 3.0 g, 2.5 g of Shiho, 2.5 g of Gilyeong, 2.5 g, Bokyeong 2.5 g, Cheongung 2.5 g, Nutritional angle 1.75 g, White worm and 1.5 g Health, and 25 g of Seokchangpo. The mixture was first extracted using a heat-circulating extractor at about 80 to 100 ° C. for 3 hours, and then filtered first when hot, and passed through No. 100 to obtain a primary extract. Further, 210 ml of water was added to the residue, followed by secondary extraction at about 80 to 100 ° C. for about 1 hour, and then filtered through cotton. Concentrated under reduced pressure.

Separately, 0.7 g of sulfur was dried well for 4 hours in a silica gel desiccator, and passed through No. 140 as a fine powder. Glue 3.5g was dissolved in 70 ml of purified water boiling water, 2.05 g of medulla, 1 g of camphor, 0.5 g of paraoxybenzoic acid methyl ester, 0.3 g of paraoxybenzoic acid propyl ester, and 5 mg of L-menthol were dissolved in 35 ml of ethanol.

In 100 ml of purified water, 350 g of D-sorbitol solution (70%), 50 g of concentrated glycerin, 50 g of sugar and 100 g of honey were added and dissolved by heating at 95 to 100 ° C. for about 15 minutes, where 0.2 g of sodium benzoate and sodium sheet were added. After adding the rate amount, stirring and dissolving it, dissolving pectin 8.75g and xanthan gum with slowly stirring and dissolving the solution was cooled to 50 DEG C or lower, while slowly stirring 10 g of cyclodextrin and 2.0 g of sodium carboxymethylcellulose. Dissolved. Here, 150 ml of the herbal extract obtained above and 0.7 g of sulfur powder, 0.2 g of silicon resin, a solution of 3.5 g of glue in hot water (about 45 ° C. or less), 2.05 g of lychee, dissolved in 35 ml of ethanol, 1 g of camphor, 0.5 g of paraoxybenzoic acid methyl ester, 0.3 g of paraoxybenzoic acid propyl ester and 5 mg of L-menthol were added and stirred for about 20 minutes, and distilled water was added to adjust the final volume to 1 L, followed by stirring for about 20 minutes. Filling a 20ml volume of the bottle with gentle stirring to prepare a periphery sulfur sulfur cheongsimwon suspension according to the present invention. The suspending agent prepared here has the same composition as Composition 1 described in Table 1 below.

Preparation Example 2 Suspending Agent Composition

Caesar 14.1g, Licorice 10.1g, White Ginseng 4.85g, Sulfur 5.0g, New Song 5.0g, Soybean Yellow Soybean 3.5g, Cinnamon 3.5g, Peony 3.0g, Macmundong 3.0g, Golden 3.0g, Angelica 3.0g, Windproof 3.0g, Baekchul Approximately 630 ml of water is added to 106.3 g of raw medicines containing 3.0 g, 2.5 g of Shiho, 2.5 g of Gilyeong, 2.5 g, Bokyeong 2.5 g, Cheongung 2.5 g, Nutritional angle 1.75 g, White worm and 1.5 g Health, and 25 g of Seokchangpo. The mixture was first extracted using a heat-circulating extractor at about 80 to 100 ° C. for 3 hours, and then filtered first when hot, and passed through No. 100 to obtain a primary extract. Further, 210 ml of water was added to the residue, followed by secondary extraction at about 80 to 100 ° C. for about 1 hour, and then filtered through cotton. Concentrated under reduced pressure.

Separately, 0.7 g of sulfur was dried well for 4 hours in a silica gel desiccator, and passed through No. 140 as a fine powder. Glue 3.5g was dissolved in 70 ml of purified water boiling water, 2.05 g of medulla, 1 g of camphor, 2 g of benzoate, 0.5 g of paraoxybenzoic acid methyl ester, 0.3 g of paraoxybenzoic acid propyl ester and 5 mg of L-menthol were dissolved in 35 ml of ethanol. .

In 100 ml of purified water, 350 g of D-sorbitol solution (70%), 50 g of concentrated glycerin, 50 g of sugar and 100 g of honey were added and dissolved by heating at 95 to 100 ° C. for about 15 minutes, where 0.2 g of sodium benzoate and sodium sheet were added. After adding the rate amount, stirring and dissolving it, dissolving pectin 8.75g and xanthan gum with slowly stirring and dissolving the solution was cooled to 50 DEG C or lower, while slowly stirring 10 g of cyclodextrin and 2.0 g of sodium carboxymethylcellulose. Dissolved. Here, 150 ml of the herbal extract obtained above and 0.7 g of sulfur powder, 0.2 g of silicon resin, a solution of 3.5 g of glue in hot water (about 45 ° C. or less), 2.05 g of lychee, dissolved in 35 ml of ethanol, 1 g of camphor, A solution of 2 g of benzoate, 0.5 g of paraoxybenzoic acid methyl ester, 0.3 g of paraoxybenzoic acid propyl ester and 5 mg of L-menthol was added and stirred for about 20 minutes, and distilled water was added to adjust the final volume to 1 L and stirred for about 20 minutes. Afterwards, a 20 ml volume vial was slowly added with stirring to prepare a milky blue suspending agent in accordance with the present invention. The suspending agent prepared here has the same composition as Composition 2 described in Table 1 below.

Ingredient pharmaceutical Composition Content Composition 1 (mg / 20ml) Composition 2 (mg / 20ml) Comparative composition (mg / 20ml) Medicine 282 282 282 licorice 202 202 202 White ginseng 97 97 97 Huang 100 100 100 new song 100 100 100 Soybean yellow 70 70 70 cinnamon 70 70 70 glue 70 70 70 Peony 60 60 60 Macmundong 60 60 60 Gold 60 60 60 Donkey 60 60 60 Windproof 60 60 60 Whiteness 60 60 60 Shiho 50 50 50 Street view 50 50 50 Passerby 50 50 50 Ghost 50 50 50 Cheongung 50 50 50 Sulfur 14 14 14 Nutrition 35 35 35 Brain 41 41 41 Whiteness 30 30 30 health 30 30 30 musk - - 5 camphor 20 20 - Seokchangpo 500 500 - Benzoin - 40 -

Preparation Example 3: Pill Composition

140.00 g of sulfur and 705 g of powdered acid were put into a grinder and ground (crude powder 1). Powder powder 2.115 ㎏, mixture licorice powder (2.02 ㎏) and nutrient cube powder (0.35 ㎏) 2.37 ㎏, white ginseng powder 970.00 g, mixture of new grain powder (1.0 ㎏) and pulmonary powder (0.6 ㎏) 1.6 kg, cinnamon powder 700.00 g , Peony Powder 600.00g, Soybean Yellow Soybean Powder 700.00g, Golden Powder 600.00g, Angelica Powder 600.00g, Windproof Powder 600.00g, Siho Powder 500.00g, Gilkyung Powder 500.00g, Baekchul Powder 600.00g, Bokryeong Powder 500.00g, Cheongung Powder 500.00 g, white crushed powder 300.00g, healthy powder 300.00g, stone powder powder 5kg, brain powder 410.00g, camphor powder 200.00g, almond powder 500.00g, turmeric powder 1.00kg, glue powder 700.00g obtained crude powder 1 above 22.71 kg of crude drug powder mixture was obtained by mixing, which was filtered through a 32 mesh sieve and then mixed into a U-type mixer.

As a binder, 15.85 kg of honey, 4.50 kg of concentrated glycerin and 40.00 g of sodium benzoate were placed in a stainless container, heated to 90 to 100 ° C. to dissolve, and impurities were removed from the upper portion to obtain a binding liquid. The crude powders were combined while adding the obtained binding liquid in small amounts to the crude powder mixture obtained above. The obtained binder was put into a ring cycle heated to a heater temperature of 85 to 95 ° C. and molded, and the molded column was switched to a ring of a constant size using a switch, then put into a pan, rotated at 18 rpm, and covered with gold leaf. To obtain 10000 of the desired mutated whey of blue cheongsimwon. The pills prepared here have the same composition as in Composition 3 described in Table 2 below.

Preparation Example 4: Pill Composition

140.00 g of sulfur and 705 g of powdered acid were put into a grinder and ground (crude powder 1). Powder powder 2115g, licorice powder (2.02㎏) and nutrient cube powder (0.35㎏) mixture 2.37㎏, white ginseng powder 970.00g, mixture of singok powder (1.0㎏) and macmundong powder (0.6㎏) 1.6kg, cinnamon powder 700.00g, Peony Powder 600.00g, Soybean Yellow Soybean Powder 700.00g, Golden Powder 600.00g, Angelica Powder 600.00g, Windproof Powder 600.00g, Siho Powder 500.00g, Gilkyung Powder 500.00g, Baekchul Powder 600.00g, Bokryeong Powder 500.00g, Cheongung Powder 500.00g , Pleurotus powder 300.00g, Healthy powder 300.00g, Seokchangpo powder 5kg, Brain powder 410.00g, Camphor powder 200.00g, Almond powder 500.00g, Benzoin powder 400.00g, Sulfur powder 1.00kg, Glue powder 700.00g obtained above The crude drug powder 1 was mixed to obtain 23.11 kg of the crude drug powder mixture, which was filtered through a 32 mesh sieve and then mixed into a U-type mixer.

As a binder, 15.85 kg of honey, 4.50 kg of concentrated glycerin and 40.00 g of sodium benzoate were placed in a stainless container, heated to 90 to 100 ° C. to dissolve, and impurities were removed from the upper portion to obtain a binding liquid. The crude powders were combined while adding the obtained binding liquid in small amounts to the crude powder mixture obtained above. The obtained binder was put into a ring cycle heated to a heater temperature of 85 to 95 ° C. and molded, and the molded column was switched to a ring of a constant size using a switch, then put into a pan, rotated at 18 rpm, and covered with gold leaf. To obtain 10000 of the desired WHwang Cheong Shim Won pills. The pills prepared here have the same composition as Composition 4 described in Table 2 below.

Ingredient pharmaceutical Composition Content Composition 3 (mg / 1 ring) Composition 4 (mg / 1 ring) Comparative composition (mg / 20ml) Medicine 282 282 282 licorice 202 202 202 White ginseng 97 97 97 Huang 100 100 100 new song 100 100 100 Soybean yellow 70 70 70 cinnamon 70 70 70 glue 70 70 70 Peony 60 60 60 Macmundong 60 60 60 Gold 60 60 60 Donkey 60 60 60 Windproof 60 60 60 Whiteness 60 60 60 Shiho 50 50 50 Street view 50 50 50 Passerby 50 50 50 Ghost 50 50 50 Cheongung 50 50 50 Sulfur 14 14 14 Nutrition 35 35 35 Brain 41 41 41 Whiteness 30 30 30 health 30 30 30 musk - - 5 camphor 20 20 - Seokchangpo 500 500 - Benzoin - 40 -

Unlike the composition of the existing sulfur sulfur cheongsimwon of the present invention, the Woobang Cheongsimwon composition of the present invention was prepared by adding the camphor and Seokchangpo, and Benzoin as needed as an alternative herbal medicine for the musk, the musk which is the main component of the sulfur sulfur simwon Although excluded, it shows the same pharmacological effect as Wu Hwang Cheong-Shim.

Such a whey of the present invention, the sulfur-free blue heart composition of the present invention exhibits significant pharmacological effects that can be used in place of the sulfur-free blue heart composition for the prevention and treatment of stroke, hypertension, palpitations, dyspnea, mental anxiety, acute gusts and autonomic ataxia. In order to confirm, the following experiment was performed. In the following experiments, the test composition means a periphery sulfur sulfur cheongsimwon composition (composition 2 of Table 1) according to the present invention prepared by the method described in Preparation Example 2, the comparative composition means a comparative composition described in Table 1.

Experimental Example 1 Effects of the Peripheral Wuhuang Cheongsimwon Composition on the Central nervous System

In order to confirm the effect of the ovarian wheys in a circle composition according to the present invention on mental anxiety or acute gusts, the existing wheys in the urine containing musk as an action on the central nervous system after oral administration of each sample to the mouse. Anticonvulsant activity against stimuli by chemicals such as, pentylenetetrazole and strikinin was measured.

In the following experiments, ICR-type male mice weighing about 25 g of body weight obtained from Korean animals were acclimated to the laboratory environment for 2 weeks under the same conditions while supplying sufficient commercial feed (Samyang oil and small animals) and water. Only healthy animals were selected and used.

The test animals and the comparative composition were orally administered to the test animals at a dose of 20.0 ml / kg, respectively, and after 30 minutes, 85.0 mg / kg of pentylenetetrazole and 1.5 mg / kg of strikinin were injected subcutaneously as convulsants. Animals with convulsions and dead animals were observed. The negative control group was orally administered with the same amount of saline, and the positive control group was orally administered phenobarbital. The measured results are shown in Tables 3 and 4, respectively.

Effect of Peripheral Sulfur Cheongsimwon Composition on Pentylenetetrazole-induced Spasm sample Dose (ml / kg, oral) The number of animals Spasms % Inhibition Saline solution 20.0 8 8 0.0 yellow jacket 48.0 mg / kg 8 1 ^** 77.5 Comparative composition 20.0 8 6 25.0 Test composition 20.0 8 3 ^** 62.5 Note) Chi-square test was used as a statistical method (** P0.01).

As can be seen from the results shown in Table 3, the negative control group exhibited a seizure suppression rate of 0.0% for the pentylenetetrazole-induced seizure, whereas a positive control group administered with phenobarbital showed 77.5% seizure inhibition rate. In addition, it was found that the test composition according to the present invention (periodical sulfur sulfur cheongsimwon) administration group exhibited a 62.5% seizure inhibition rate, which was significantly superior to the comparative composition administration group (25.0% seizure inhibition rate).

Influence of Peripheral Wheys on the Strikinin-induced Spasm sample Dose (ml / kg, oral) The number of animals Dead animals % Inhibition Saline solution 20.0 8 6 25.0 yellow jacket 48.0 mg / kg 8 0 ^** 100.0 Comparative composition 20.0 8 4 50.0 Test composition 20.0 8 4 50.0 Note) Chi-square test was used as a statistical method (** P0.01).

As can be seen from the results shown in Table 4, the negative control group treated with physiological saline for strikinin-induced convulsions showed a seizure inhibition rate of 25.0%, whereas the positive control group treated with phenobarbital had 100.0% seizure. Inhibition rate was shown, and both the test composition (negative sulphate cheongsimwon) and the comparative composition according to the present invention showed a seizure inhibition rate of 50.0%, indicating that there was no significant difference between the effects of the two compositions.

Taken together, the results of the present invention suggest that the periphery sulfur sulfur cheongsimwon composition according to the present invention exhibits an anticonvulsive action equivalent to or higher than that of the sulfur sulfur cheongsimwon (composition) containing musk. Therefore, the mutated whey of the present invention composition of the present invention can be effectively used as an alternative to the sulfur of the blue won in treating symptoms such as palpitations, respiratory distress, mental anxiety, acute gusts and autonomic ataxia.

Experimental Example 2 Effect of Peripheral Whey Cheongsimwon Composition on SHR Hypertension

In order to evaluate the high blood pressure therapeutic effect of the mutated milk sulfur cheongwon circle composition of the present invention, spontaneously hypertensive rat (SHR) as a hypertension model was performed as follows.

The experimental animals were adapted to the laboratory environment by supplying male Spontaneous hypertension rats (SHR) of 200-250g body weight (SHR) and water. Rats that were no longer increased to about 350 g body weight were adapted to blood pressure training for 4 weeks, and then divided into 4 groups. As a sample, 4 ml / kg of saline was orally administered to the control group (9 animals), and 0.4 mg / kg of captopril was administered orally to the positive control group (6 animals). The test composition of the invention (test composition pretreatment group: number of test animals 9) and the comparative composition (comparison composition pretreatment group: number of test animals 9) were orally administered at a dose of 4 ml / kg. Blood pressure was measured at 30 minutes, 1 hour, 2 hours, 4 hours, and 8 hours before and after administration of the sample, respectively. The blood pressure was measured using a tail cuff method for measuring blood pressure in the tail artery of rats.Then, it was connected to a transducer and then connected to a polygraph (grass model 7J, USA) and an amplifier (amplifier, sphygmomanometer). EKG-Pulse Preamplifier 7P8K, Grass Polygraph DC Driver Amplifier, USA) was recorded and the results obtained were analyzed via Maclab, ML / 8e, USA. The blood pressure change was calculated by calculating the measured blood pressure value according to the following equation.

The results are shown in FIG. As can be seen from the results shown in FIG. 1, in the spontaneous hypertension rats, the periphery of the sulfur-free blue core composition according to the present invention showed a more significant blood pressure lowering effect than the comparative composition, and this effect was 6 hours after 1 hour of administration. It showed a tendency to last. Therefore, it can be seen that the composition of the present invention can be used as an effective surrogate for bovine sulfur cheongsimwon against high blood pressure.

Experimental Example 3 Effects of Peripheral Whey Cheongsimwon Composition on Blood Clotting Time and Thrombosis

In order to evaluate the prophylactic and therapeutic effect of stroke of the present invention, the composition of the whey of blue urine was observed orally administered to rats, and the effects of clotting time and thrombus were observed. As experimental animals, male mice with a weight of 25g and male rats with a weight of 200g and Sprague-Dawley male rats were supplied with commercial solid feeds (for Samyang oil and small animals) and water, and then adapted to the laboratory environment for 2 weeks. Selected and used.

(1) Effect on clotting time

Using a saline solution (9 animals), a test composition (7 animals), and a comparative composition (9 animals), each sample was orally administered to the experimental animal at a dose of 4 ml / kg and 30 minutes. Later, blood was collected from capillaries (Chase, USA) from the orbital vein of mice, and the time until clots were formed was measured. The measured result is shown in FIG.

From the results shown in FIG. 2, the coagulation time in the control group was 59.9 ± 4.6 seconds, and the coagulation time was delayed to 72.0 ± 5.9 seconds in the comparative composition-treated group, and even in the test composition-administered group of the present invention, 72.0 ± 2.2 seconds. It can be seen that the clotting time was significantly extended (p <0.05).

(2) effects on blood clots

As a sample, each group was orally administered to rats, which are experimental animals, at a dose of 10.0 ml / kg, using physiological saline (normal group, control group), test composition, and comparative composition, and endotoxin 0.1 mg to test animals except the normal group after 30 minutes. / Kg was injected into the tail vein. After 4 hours, blood was collected by cardiac puncture, and platelet count, prothrombin time and fibrinogen amount were measured according to the following method.

Platelet counting

Platelet counting was performed using the Ponio method [Fonio A .; Acta Haematol. 1966, 36: 5, 371-85. 14% magnesium sulfate and blood were mixed to a level of about 3: 1 and plated on slide glass and dried. Fix for 5 minutes with methanol and mix 1 drop of Giemsa solution with 1.0 ml of water, fix dyeing for 1 hour, count red blood cells and platelets in each field with a visual reducer, count up to 1,000 red blood cells and count the number of platelets for 1,000 red blood cells. The platelet count P in 1.0 dl was computed according to the following formula, with the red blood cell number which exists in 1.0 dl of blood as R.

The measured results are shown in Table 5 below.

Influence of Peripheral Whey Blue and Green Circle Composition on Endotoxin-induced Coagulation sample Dose (ml / kg, oral) The number of animals Platelet count (× 10 ⁴ / mm3) Normal 10.0 9 817.9 ± 40.8 ^a) Control 10.0 7 691.4 ± 33.2 Comparative composition 10.0 10 745.2 ± 35.9 Test composition 10.0 9 741.1 ± 29.8 Note) (1) Physiological saline was administered to the normal group and the control group. Normal group was not treated with endotoxin. Control and comparative compositions were treated with endotoxin. (2) a) Mean ± standard error (3) Statistical method used the Student's t- method.

As can be seen from the results described in Table 5, the platelet count of the normal group was 817.9 ± 40.8 (× 10 ⁴ / mm 3), whereas the control group decreased to 691.4 ± 33.2 (× 10 ⁴ / mm 3). On the other hand, in the experimental animals administered the comparative composition as a sample, the platelet count was 745.2 ± 35.9 (× 10 ⁴ / ㎣), and the decrease in platelet count was suppressed compared to the control group, and in the group administered the test composition, 741.1 ± 29.8 (× 10 ^4). / ㎣) significantly reduced the platelet count compared to the control group.

Prothrombin Time Measurement

Prothrombin time was measured using a simplastin kit (General Diagnotics). 0.1 ml of citrate plasma, which is mixed with 1 volume of 3.2% sodium citrate and 9 volumes of blood, is taken in a small test tube and placed in a constant temperature water bath at 37 ° C for about 3 minutes, and 0.2 ml of simplastine kept at the same temperature is added to the stopwatch. After starting and shaking gently, the time until the fibrin precipitated was measured. The measured results are shown in Table 6 below.

Influence of Peripheral Whey Cheongsimwon Composition on Prothrombin Time sample Dose (ml / kg, oral) The number of animals Prothrombin time in seconds Normal 10.0 9 16.3 ± 0.6 ^a) Control 10.0 7 19.3 ± 0.7 Comparative composition 10.0 10 16.5 ± 0.3 Test composition 10.0 9 16.9 ± 0.3 ^** Note) (1) Physiological saline was administered to the normal group and the control group. The normal group was not treated with endotoxin. (2) a) Mean ± standard error (3) The statistical t-method was used (* P0.05).

As can be seen from the results described in Table 6, the prothrombin time of the normal group was increased to 19.3 ± 0.7 seconds compared to 16.3 ± 0.6 seconds. On the other hand, prothrombin time was 16.5 ± 0.3 seconds in the experimental animals administered the comparative composition as a sample, and prolongation of prothrombin time was suppressed compared to the control group, and the group treated with the test composition was significantly 16.9 ± 0.3 seconds, similar to the control group. Prolongation of prothrombin time was inhibited.

Determination of fibrinogen levels

Fibrinogen levels in plasma were measured using a Fibrinogen test set (Medichem). Test plasma was diluted 10-fold with olein buffer, 0.2 ml of the test tube was warmed for 3 minutes, and then 0.1 ml of room temperature thrombin was added to measure the coagulation time, and the concentration of fibrinogen was determined from the calibration curve. The measured results are shown in Table 7 below.

Influence of Peripheral Sulfur Cheongsimwon Composition on Fibrinogen Amount sample Dose (ml / kg, oral) The number of animals Fibrinogen amount (mg / dl) Normal 10.0 9 273.7 ± 16.4 ^a) Control 10.0 7 231.8 ± 15.9 Comparative composition 10.0 10 244.9 ± 11.8 Test composition 10.0 9 247.3 ± 9.5 Note) (1) Physiological saline was administered to the normal group and the control group. The normal group was not treated with endotoxin. (2) a) Mean ± standard error (3) The statistical t-method was used.

As can be seen from the results shown in Table 7, the amount of fibrinogen in the normal group was 273.7 ± 16.4 mg / dL, whereas the control group decreased to 231.8 ± 15.9 mg / dL. On the other hand, in the experimental animals administered with the comparative composition as a sample, the decrease in the amount of fibrinogen was inhibited as compared with the control group, with the amount of fibrinogen being 244.9 ± 11.8 mg / dl, and in the group treated with the test composition as 247.3 ± 9.5 mg / dl Reduction of the amount of fibrinogen was suppressed to the same extent as.

From the results of the above experiments, the fenestral, sulfur, and benzoic compositions of the present invention, which do not contain musk, but instead contain camphor, changchangpo, and benzoate, prolong blood coagulation time to the same extent as that of the sulphurung containing the musk, and have a significant antithrombotic effect. It can be seen that, therefore, it can be seen that it can be used as an excellent drug that can be substituted for the Wowang Cheongsimwon in the prevention and treatment of stroke caused by blood clots.

Claims (4)

Based on the weight of the active powder, acid powder 190-370, licorice 140-270, white ginseng 60-130, turmeric 70-130, singok 70-130, soybean yellow peach 40-100, cinnamon 40-100, glue 40-100, Peony 30-90, Mcmundong 30-90, Golden 30-90, Angelica 30-90, Windproof 30-90, Baekchul 30-90, Sea Ho 30-70, Gil Gyeong 30-70, Pedestrian 30-70, Bokryeong 30-70, Peripheral sulfur sulfur cheongsimwon composition composed of the ratio of cheongung 30-70, cow sulfur 10-20, nutrition angle 20-50, cephalophyte 20-60, baekryeom 15-45, health 15-45, camphor 10-30 and Seokchangpo 300-700. The method of claim 1, wherein further comprising the benzoate in a ratio of 20-60 by weight based on the weight of the perilla milk sulfur cheongsimwon composition. The method of claim 1 or 2, wherein the periphery milk sulfur cheongwon circle composition is a liquid formulation. The method of claim 1 or 2, wherein the yeonbohwang cheongsimwon composition in the form of a pill.