KR19980076652A - Epithelial extract with antimicrobial activity on food - Google Patents

Abstract

The present invention adds an epithelial extract obtained by extracting mulberry bark (epithelius) with organic solvents such as ethanol, chloroform, ethyl acetate, butanol, etc. to improve the shelf life of foods that provide antimicrobial activity against microorganisms causing food poisoning. There is a characteristic. In the distribution of refrigerated foods requiring freshness of food, high temperature sterilization, freezing, chemical synthetic additives and the like are used as a means for extending the preservation of food.

However, the epidermal extract of the present invention aims to provide a natural antimicrobial active substance that can extend the preservation of food as a natural ingredient.

Description

Epithelial extract with antimicrobial activity on food

The present invention relates to a water-soluble extract of mulberry bark (hereinafter referred to as epidermis) having an antimicrobial activity effect of food and a method for producing the same.

As the income level develops, foods are becoming more advanced and diversified. In order to improve shelf life in processed foods, methods of sterilizing at high temperatures or adding synthetic preservatives have become common, but they have been a factor deteriorating the commerciality.

The sterilization method at high temperature causes the deterioration of the nutritional and trace ingredients of the food itself due to excessive heat treatment.

In addition, synthetic preservatives are harmful to the human body, the amount of use is strictly regulated by the Food Sanitation Law, but there is a disadvantage that does not meet the needs of consumers who prefer natural foods.

Frozen sterilization can completely prevent microbial contamination, but deterioration of quality due to freezing is a big problem.

The present invention has been developed a method for extending the shelf life of food without impairing the taste and function of the food by adding the epithelial extract having an antimicrobial activity to the refrigerated food.

Epithelium has been used as an herbal medicine because of its glycemic effect, but has not been used in the antimicrobial effect.

Conventional food sterilization or preservation as a means of extending the high temperature sterilization, freezing, the addition of synthetic preservatives, but there was a disadvantage that can not maintain the intrinsic commerciality of food.

Psychrotrophs are commonly known as food poisoning microorganisms that can be grown at low temperature below 10 ° C when food is distributed in the market. These are known and they all grow at low temperatures, causing food poisoning.

Therefore, thorough temperature control and shelf life compliance to prevent food poisoning by microorganisms in refrigerated foods is important, and various efforts have been made to use natural products without using synthetic preservatives to improve the commerciality of food. The present invention is expected to be able to improve the shelf life and commerciality of food as a natural extract and a method for producing the same that can solve the above problems.

1) Extraction of Active Ingredients

Mix 5 times 75% ethanol with the ground epithelium sample ground with a grinder, and place it in a flask equipped with a reflux condenser. Heat extraction and filtration for 3 hours in an 85 ℃ water bath to evaporate ethanol in a rotary vacuum evaporator to obtain an extract. Solid portion was taken as 1 ml of concentrate and dried at 105 ° C. to obtain the evaporation residue.

2) fraction of extract

The extract was partitioned sequentially using chloroform, ethyl acetate, butanol, and water using Separating Funnel. That is, 10 ml of crude extract was added to 40 ml of chloroform, shaken for 10 minutes, and left for 30 minutes to collect a chloroform layer. The tissue was repeatedly collected twice to remove the chloroform layer, and the remaining material was dissolved in water.

In this way, ethyl acetate, butanol, and water-soluble substances were separated.

3) Determination of antimicrobial activity

Each test strain incubated in Disk Diffusion Method Slant was inoculated into 10 ml tryptic soy broth, incubated at 32 ° C for 24 hours to activate, and 0.1 ml of the culture solution was dropped on a dry tryptic soy agar plate overnight at room temperature and then bent. The glass rod was applied uniformly. A 0.65mm filter disc was placed on the plate inoculated with each test organism, and the antimicrobial activity was compared by the diameter of the clear zone (mm) around the disc after 48 hours of incubation at 32 ° C.

Table 1. Antimicrobial Activities of Epithelial Extracts Extracted with 75% Ethanol Solution

1) Soluble soild (mg) / disk

2) Clear zone (mm)

As shown in Table 1, the 75% ethanol epithelial extract showed high antimicrobial activity by forming more than 15mm clear zone for all 5 L monocytogenes, and especially the highest antimicrobial activity by forming a 21mm clearzone against ATCC 19113 strain. It was. Their results show similar antimicrobial activity to L. monocytogenes of epithelial extracts in a similar trend to previous results.

Example 1

100 g of epithelial sample was placed in 500 ml of 75% ethanol, extracted by heating for 3 hours in a flask attached to a reflux condenser on an 85 ° C. water bath, filtered, and evaporated to ethanol in a rotary vacuum evaporator to obtain a crude extract.

The extract was added with 10 ml of crude extract in 40 ml of chloroform and Separating Funnel, shaken for 10 minutes, and left for 30 minutes to collect the chloroform layer. This operation was repeated twice to remove the collected chloroform layer, and the remaining substance was dissolved in water. An extract was obtained.

Example 2

The crude extract was obtained in the same manner as in Example 1, and the extract was obtained using ethyl acetate as a solvent. Antimicrobial activity of the extract was as in Example 1.

Example 3

A crude extract was obtained as in Example 1, and an extract was obtained using butanol as a solvent.

Example 4

In order to measure the antimicrobial activity of the extracts obtained in Examples 1, 2 and 3, each test strain cultured in Slant by disk diffusion method ATCC 19111, ATCC 19112, ATCC 19113, ATCC 15313 (prepared by Listeria monocytogenes National Institute of Health)

1 platinum was inoculated on tryptic soy broth and incubated for 24 hours at 32 ° C. to activate the solution, and 0.1 ml of the culture solution was dropped on a tryptic soy agar plate dried overnight at room temperature, and then uniformly applied with a bent glass rod. 0.65mm filterdisc absorbed extract was placed on the plate inoculated with each test organism, and the antimicrobial activity was compared by the diameter of the clear zone (mm) around the disc after 48 hours incubation at 32 ℃.

Example 5

Epithelium was extracted from 75% ethanol solution and added to 2000ppm of pasteurized paste which was sterilized by membrane filter. After 24 hours, the number of bacteria was significantly decreased compared to the initial number.

Table 2. Antimicrobial Activities of Epithelial Extracts on Food Poisoning Microorganisms

No content.

Claims (2)

Epithelial extract, characterized in that the extraction of the epithelium with an organic solvent has an antimicrobial activity in food The epithelial extract of claim 1, wherein the organic solvent is ethanol, chloroform, ethyl acetate, butanol.