KR102695644B1 - Paste composition for companion animals and method thereof - Google Patents

Abstract

The present invention relates to a paste composition for companion animals, which comprises both water-soluble and fat-soluble functional ingredients while minimizing the use of surfactants and ensuring physicochemical stability, and a method for producing the same, the paste composition comprising an aqueous phase comprising a water-soluble functional ingredient, an oil phase comprising a fat-soluble functional ingredient, a surfactant, saturated fatty oil, and protein, and the surfactant may be included in an amount of 0.1 to 5 wt% based on the total amount of the composition.

Description

{Paste composition for companion animals and method thereof}

The present invention relates to a paste composition for companion animals and a method for producing the same, and more specifically, to a paste composition for companion animals which includes both water-soluble and fat-soluble functional ingredients while minimizing the use of surfactants and ensuring physicochemical stability, and a method for producing the same.

Recently, due to the increase in single-person households, low birth rates, and aging population, the number of households living with pets has been continuously increasing, and the tendency to recognize pets as members of the family is growing stronger.

Accordingly, as interest in the well-care of companion animals grows, various pet foods including feed, supplementary feed, snacks such as gum, beverages, and nutritional supplements containing various functional ingredients are being released.

In particular, most pet nutritional supplements containing functional ingredients are manufactured in tablet form, which makes it difficult to administer them directly to pets. Even if they are mixed with feed and administered, this is also cumbersome and the mixing with the feed is not done well, resulting in low efficiency. Considering these problems, a nutritional supplement in the form of a paste can be proposed as a way to improve the convenience of administration.

However, when manufacturing a paste-type composition containing various functional ingredients, there are various factors that harm the physicochemical stability of the active ingredients and the paste itself, and solutions for these must be sought.

For example, if a paste composition is intended to contain both water-soluble and fat-soluble components as functional ingredients, the dispersion media for each component may separate from each other over time. In addition, if the physical stability of the functional ingredients is not secured, phase separation, precipitation, and coagulation may occur during the storage period, and problems such as chemical decomposition may occur, resulting in a decrease in the content.

Meanwhile, a large amount of surfactant can be used to prevent phase separation of functional ingredients and dissolve poorly dissolved ingredients, but the more surfactant is used, the more side effects in the gastrointestinal tract and skin diseases may occur after ingestion, and the characteristic bitter taste may have the adverse effect of reducing palatability.

The purpose of the present invention is to provide a paste composition for companion animals which includes both water solubility and fat solubility as a functional ingredient, while minimizing the use of a surfactant and ensuring physicochemical stability, and a method for producing the same.

The pet paste composition according to the present invention for solving the above-described technical problem comprises an aqueous phase including a water-soluble functional ingredient, an oil phase including a fat-soluble functional ingredient, a surfactant, saturated fatty oil and protein, and the surfactant may be included in an amount of 0.1 to 5 wt% based on the total amount of the composition.

In addition, the water-soluble functional ingredient may include glucosamine, collagen, and water-soluble vitamins, and the fat-soluble functional ingredient may include lutein and fat-soluble vitamins.

In addition, the water phase may be included in an amount of 1 to 5 wt%, the oil phase in an amount of 10 to 60 wt%, the saturated fatty oil in an amount of 5 to 35 wt%, and the protein in an amount of 10 to 40 wt%, based on the total amount of the composition.

Additionally, it may further comprise one or more additives selected from the group consisting of sweeteners, flavoring agents and preservatives.

In addition, the method for manufacturing a paste composition for companion animals according to the present invention may include: (1) a step of preparing an oil phase in which vegetable oil and a fat-soluble functional ingredient are mixed, and an aqueous phase in which water and a water-soluble functional ingredient are mixed; (2) a step of mixing saturated fatty oil into the oil phase prepared in step (1); (3) a step of mixing a surfactant into the oil phase prepared in step (2); (4) a step of mixing and dispersing vegetable protein into the oil phase prepared in step (3); (5) a step of mixing and dispersing the aqueous phase prepared in step (1) into the oil phase prepared in step (4); and (6) a step of homogenizing the composition prepared in step (5).

According to the companion animal paste composition according to the present invention, physicochemical stability can be secured, and side effects that may be caused by minimizing the amount of surfactant used can be reduced, while at the same time providing high palatability as a nutritional supplement for companion animals.

Figure 1 is a flow chart showing a method for manufacturing a paste composition for companion animals according to an embodiment of the present invention.
Figure 2 is a photograph showing the properties of a paste composition immediately after preparation according to an experimental example of the present invention and after storage for 5 months at 40°C.

Each description and embodiment disclosed in this application can also be applied to each other description and embodiment. That is, all combinations of various elements disclosed in this application fall within the scope of the present invention. In addition, the scope of the present invention cannot be said to be limited by the specific description described below.

Furthermore, those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described in this application. Such equivalents are also intended to be encompassed by the present invention.

Additionally, throughout the specification of this application, when a part is said to "include" a certain component, this does not mean that other components are excluded, but rather that other components may be included, unless otherwise specifically stated.

Hereinafter, the present invention will be described in more detail.

The pet paste composition according to the present invention may include an aqueous phase containing a water-soluble functional ingredient, an oil phase containing a fat-soluble functional ingredient, an emulsifier or surfactant, saturated fatty oil, and protein, and may further include one or more additives selected from the group consisting of a sweetener, a flavoring agent, and a preservative.

In specific content, the oil phase may be included in an amount of 10 to 60 wt%, the surfactant in an amount of 0.1 to 5 wt%, the water phase in an amount of 1 to 5 wt%, the saturated fatty oil in an amount of 5 to 35 wt%, the protein in an amount of 10 to 40 wt%, the sweetener or flavoring agent in an amount of 0.01 to 0.1 wt%, and the preservative in an amount of 0.01 to 0.1 wt%, based on the total amount of the composition.

Functional ingredients can be divided into water-soluble active ingredients and fat-soluble active ingredients. Water-soluble active ingredients can include glucosamine, collagen, and water-soluble vitamins (B1_thiamine, B2_riboflavin, B3_nicotinamide, B5_pantothenic acid, B6_pyridoxine, B7_biotin, B9_folic acid, B12_cyanocobalimin, C_ascorbic acid), and fat-soluble active ingredients can include lutein and fat-soluble vitamins (A_retinol, D_cholicalciferol, E_tocopherol).

As for the efficacy of functional ingredients, glucosamine prevents joint diseases such as arthritis and osteoarthritis, strengthens the bones, muscles, and cartilage of companion animals, and prevents patellar dislocation. In addition, collagen is a substance that functions as the main protein of all connective tissues in the body such as skin, blood vessels, and bones, and can prevent immune deficiency, skin diseases, and hair loss that often occur in companion animals that mainly live indoors. In addition, lutein is a substance that is good for preventing macular degeneration and cataracts, and helps maintain healthy eyes by protecting eye cells, and helps protect the eyes from oxidative stress and high-energy photons of blue light.

Since companion animals cannot produce essential fatty acids such as omega 3 and omega 6 in their bodies, they must ingest them through their diet. To supplement this, vegetable oil was selected as the composition of the oil phase. The vegetable oil may be selected from olive oil, canola oil, linseed oil, corn oil, sunflower seed oil, grape seed oil, walnut oil, cottonseed oil, avocado oil, Brazil nut oil, and almond oil, but is not limited thereto. Preferably, the vegetable oil may be included in an amount of 10 to 60 wt% based on the total amount of the composition. If the content exceeds this range, phase separation may occur after paste manufacture or during storage.

Surfactants or emulsifiers are used to reduce the surface tension between two substances that are difficult to mix among the substances added to the paste by utilizing the property of emulsifying oil and water, and to evenly disperse them. Available emulsifiers include sorbitan fatty acid ester, glycerin fatty acid ester, sucrose fatty acid ester, propylene glycol fatty acid ester, polysorbate (polyoxyethylene sorbitan fatty acid ester, tween 60 or 80), lecithin, saponin, etc. The daily allowable standards for emulsifiers are 0 to 125 mg/kg for glycerin fatty acid esters, 0 to 30 mg/kg for sucrose fatty acid esters, 0 to 20 mg/kg for propylene glycol fatty acid esters, and 0 to 25 mg/kg for polyoxyethylene sorbitan fatty acid esters (polysorbates). If ingested in amounts exceeding a certain amount, these emulsifiers can remain in the body for more than 5 days and can cause skin diseases, eye diseases, heart diseases, neuropsychiatric diseases, etc. In order to reduce these problems and improve physical stability, plant-based emulsifiers were included, and 0.1 to 5 wt% was included in the total amount of the composition, which is a small amount compared to the emulsifier content in general health functional foods.

Saturated fat oil is used to improve the physical stability of the paste, such as viscosity and sedimentation, and to increase the palatability of companion animals. Available saturated fat oils include palm oil, shea butter, coconut oil, argan oil, and soybean oil. Preferably, the saturated fat oil can be mixed in an amount of 5 to 35 wt% based on the total amount of the composition, and if the content of the saturated fat oil is out of the above numerical range, it is difficult to secure physical stability, such as phase separation, sedimentation, and coagulation.

The protein is a protein powder processed from soybeans, black soybeans, kidney beans, etc., and is used for the purpose of improving physical stability such as phase separation, precipitation, and coagulation, and increasing the nutritional supply to companion animals through protein supply. Available proteins include soy protein isolate ISP processed from beans, Supro 661, and defatted soybean powder. Preferably, the vegetable proteins may be included in an amount of 10 to 40 wt% based on the total amount of the composition. If the protein content is lower than that specified above, phase separation may occur and physical stability may deteriorate.

The paste composition according to the present invention may further comprise acceptable additives. For example, at least one selected from the group consisting of a sweetener, a flavoring agent, and a preservative may be included.

The above flavoring agent is an additive that allows a sense of flavor and can improve convenience. For example, one or more substances selected from meat flavor, smoked flavor, various fruit flavors, nut flavors, etc. can be used. The flavoring agent can be included in an amount of 0.01 to 0.1 wt%, preferably 0.01 to 0.05 wt%, based on the total amount of the composition, but is not limited thereto.

The above preservative is an additive capable of inhibiting the growth of microorganisms during storage of the composition, and for example, one or more substances selected from potassium sorbate, sodium benzoate, methyl paraoxybenzoate, propyl paraoxybenzoate, etc. may be used. The preservative may be used in an amount of 0.01 to 1 weight part (%), preferably 0.01 to 0.1%, based on the total amount of the composition.

A paste composition for companion animals according to an embodiment of the present invention can be manufactured by the following method.

Referring to Figure 1, a method for manufacturing a paste composition for companion animals is provided.

(a) a step of preparing an oil phase by mixing vegetable oil and fat-soluble components, and an aqueous phase by mixing water and water-soluble components;

(b) a step of mixing the mixed oil phase in (a) by including saturated fat in it;

(c) a step of mixing an emulsifier or surfactant into the mixed oil phase in (b);

(d) a step of dispersing vegetable protein into the mixed oil phase in the above (c);

(e) a step of dispersing the aqueous phase prepared in (a) into the mixed phase in (d);

(f) It may include a step of homogenizing the mixed composition in (e) using a homogenizer, mixer, etc.

Hereinafter, embodiments of the present invention will be described in more detail. However, these embodiments are intended to exemplify the present invention, and the scope of the present invention is not limited by these embodiments, as will be apparent to those skilled in the art to which the present invention pertains.

Example: Preparation of paste composition for companion animals

<Examples 1 to 20>

Companion animal paste compositions of Examples 1 to 20 were prepared with the compositions and contents shown in Tables 1 and 2 below.

Specifically, vegetable oil, lutein raw material in oil form, and fat-soluble vitamins (A, D, E) were added to a beaker and stirred, and saturated fat and an emulsifier or surfactant were added and stirred for 15 minutes to mix. Additionally, proteins were added and stirred to disperse the proteins, and an aqueous phase (water) containing glucosamine, collagen, water-soluble vitamins, flavoring, and preservatives was added and mixed. To homogenize the compositions, a paste formulation was prepared by homogenizing at 15,000 rpm for 10 minutes using a homogenizer (IKA Homogenizer, Germany).

Composition (%) Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 Example 9 Example 10 Canola oil 35 - - 36 - - 45 - - 50 Grapeseed oil - 35 - - 45 - - 50 - - olive oil - - 30 - - 49 - - 40 - lecithin 2 2 2 - - 5 2 2 - - Glycerin fatty acid ester 0.1 0.1 1 0.5 0.5 - 1 - 2 3 Polysorbate 80 - - - 2 2 - - 0.1 - - Coconut oil 32 32 35 15 23 10 10 15 20 15 Soup 661 20 20 20 35 20 25 - - - 20 Isolated soy protein ISP - - - - - - 30 20 25 - water 2.5 2.5 2.5 2.5 2.5 2.5 2.5 2.5 2.5 2.5 glycerin 5 5 5 5 5 5 5 5 5 5 Spices 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Glucosamine hydrochloride 3.35 - - 3.35 - - 3.35 3.35 3.35 - Lutein Oil - 0.02 - - - 0.02 - - - 0.02 low molecular collagen - - 0.15 - 0.15 - - - - - Vitamin B1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Vitamin B2 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 Vitamin B3 0.00009 0.00009 0.00009 0.00009 0.00009 0.00009 0.00009 0.00009 0.00009 0.00009 Vitamin B5 0.024 0.024 0.024 0.024 0.024 0.024 0.024 0.024 0.024 0.024 Vitamin B6 0.0029 0.0029 0.0029 0.0029 0.0029 0.0029 0.0029 0.0029 0.0029 0.0029 Vitamin B7 0.00021 0.00021 0.00021 0.00021 0.00021 0.00021 0.00021 0.00021 0.00021 0.00021 Vitamin B9 0.00045 0.00045 0.00045 0.00045 0.00045 0.00045 0.00045 0.00045 0.00045 0.00045 Vitamin B12 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 Vitamin C 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 sodium selenite 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 Vitamin A 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 Vitamin D 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 Vitamin E 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Potassium Sorbate 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Total(%) 100 100 100 100 100 100 100 100 100 100

Composition (%) Example 11 Example 12 Example 13 Example 14 Example 15 Example 16 Example 17 Example 18 Example 19 Example 20 Canola oil 45 - - - 50 45 35 35 - - Grapeseed oil - - 38 - - - - - 35 - olive oil - 55 - 46 - - - - - 35 lecithin 2 2 2 2 2 2 2 2 2 2 Glycerin fatty acid ester 1 1 - 2 3 1 0.1 0.1 - - Polysorbate 80 - - 0.1 - - - - - 0.1 0.1 Coconut oil 20 5 20 15 5 15 32 32 32 32 Soup 661 23 25 30 - - 15 20 22.5 10 10 Isolated soy protein ISP - - - 25 30 13 5 - 12.5 12.5 water 1 3 2.5 1.5 5 1 2.5 2.5 2.5 2.5 glycerin 5 5 5 5 5 5 - 2.5 2.5 2.5 Spices 0.05 0.05 - - 0.05 0.05 0.05 0.05 0.05 0.05 Glucosamine hydrochloride - - - - - 3.35 3.35 - - - Lutein Oil 0.02 0.02 - - - - - 0.02 0.02 - low molecular collagen - - 0.15 0.15 0.15 - - - - 0.15 Vitamin B1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Vitamin B2 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 Vitamin B3 0.00009 0.00009 0.00009 0.00009 0.00009 0.00009 0.00009 0.00009 0.00009 0.00009 Vitamin B5 0.024 0.024 0.024 0.024 0.024 0.024 0.024 0.024 0.024 0.024 Vitamin B6 0.0029 0.0029 0.0029 0.0029 0.0029 0.0029 0.0029 0.0029 0.0029 0.0029 Vitamin B7 0.00021 0.00021 0.00021 0.00021 0.00021 0.00021 0.00021 0.00021 0.00021 0.00021 Vitamin B9 0.00045 0.00045 0.00045 0.00045 0.00045 0.00045 0.00045 0.00045 0.00045 0.00045 Vitamin B12 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 Vitamin C 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 sodium selenite 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 Vitamin A 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 Vitamin D 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 Vitamin E 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Potassium Sorbate 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Total(%) 100 100 100 100 100 100 100 100 100 100

<Comparative examples 1 to 7>

Comparative examples 1 to 10 of the pet paste compositions are prepared with the compositions and contents shown in Table 3 below.

The manufacturing method is similar to the manufacturing method of Examples 1 to 20 above.

Composition (%) Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Comparative Example 9 Comparative Example 10 Canola oil 45 - - 60 - - 35 35 28 - Grapeseed oil - 45 - - 55 - - - - - olive oil - - 20 - - 20 - - - - lecithin 2 2 2 - - 1 2 2 2 - Glycerin fatty acid ester 0.1 0.1 1 0.5 0.5 - 1 1 1 1 Polysorbate 80 - - - 2 2 - - - - 2 Coconut oil - 2.5 50 25 25 20 - - - 35 Soup 661 40 40 15 10 5 50 - 50 50 45 Isolated soy protein ISP - - - - - - 50 - - - water 2.5 2.5 2.5 2.5 2.5 2.5 2.5 5 10 10 glycerin 5 5 5 5 5 5 5 5 5 - Spices 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Glucosamine hydrochloride 3.35 - - 3.35 - - 3.35 - - 3.35 Lutein Oil - 0.02 - - 0.02 - - 0.02 - - low molecular collagen - - 0.15 - - 0.15 - - 0.15 - Vitamin B1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Vitamin B2 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 Vitamin B3 0.00009 0.00009 0.00009 0.00009 0.00009 0.00009 0.00009 0.00009 0.00009 0.00009 Vitamin B5 0.024 0.024 0.024 0.024 0.024 0.024 0.024 0.024 0.024 0.024 Vitamin B6 0.0029 0.0029 0.0029 0.0029 0.0029 0.0029 0.0029 0.0029 0.0029 0.0029 Vitamin B7 0.00021 0.00021 0.00021 0.00021 0.00021 0.00021 0.00021 0.00021 0.00021 0.00021 Vitamin B9 0.00045 0.00045 0.00045 0.00045 0.00045 0.00045 0.00045 0.00045 0.00045 0.00045 Vitamin B12 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 Vitamin C 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 sodium selenite 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 0.0005 Vitamin A 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 Vitamin D 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 Vitamin E 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Potassium Sorbate 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Total(%) 100 100 100 100 100 100 100 100 100 100

Experimental Example 1: Observation of physical stability of paste composition for companion animals

Each of the compositions of Examples 1 to 20 and Comparative Examples 1 to 10 was stored at 40°C for 5 months, and then visually evaluated for phase separation, precipitation, coagulation, and foreign matter formation using the following test method.

1) Take out the sample at 40℃ and mix evenly.

2) Store the solution in 1) at room temperature for 30 minutes.

3) Visually observe the liquid in 2) to visually evaluate whether there is phase separation, precipitation, coagulation, or formation of foreign substances.

The results are shown in Table 4 and Fig. 2. Fig. 2 representatively shows the properties immediately after manufacturing of Examples 1, 2, and 3 and Comparative Examples 1, 2, and 3 (A) and the properties after storage for 5 months at 40°C (B).

As a result, Examples 1 to 20 of the various paste compositions manufactured according to the present invention all had the active ingredient and other ingredients evenly or uniformly dispersed, and maintained the even dispersion even under storage conditions of 40° C. and storage conditions for 5 months.

On the other hand, some of the compositions of Comparative Examples 1 to 10 were found to have properties in which the components were not evenly dispersed or coagulated immediately after manufacturing, and were all found to be phase separated and coagulated under storage conditions at 40°C for 5 months, indicating that they were unsuitable for formulation design.

Properties immediately after manufacturing Characteristics after 5 months of storage at 40℃ Example 1 Evenly distributed Evenly distributed Example 2 Evenly distributed Evenly distributed Example 3 Evenly distributed Evenly distributed Example 4 Evenly distributed Evenly distributed Example 5 Evenly distributed Evenly distributed Example 6 Evenly distributed Evenly distributed Example 7 Evenly distributed Evenly distributed Example 8 Evenly distributed Evenly distributed Example 9 Evenly distributed Evenly distributed Example 10 Evenly distributed Evenly distributed Example 11 Evenly distributed Evenly distributed Example 12 Evenly distributed Evenly distributed Example 13 Evenly distributed Evenly distributed Example 14 Evenly distributed Evenly distributed Example 15 Evenly distributed Evenly distributed Example 16 Evenly distributed Evenly distributed Example 17 Evenly distributed Evenly distributed Example 18 Evenly distributed Evenly distributed Example 19 Evenly distributed Evenly distributed Example 20 Evenly distributed Evenly distributed Comparative Example 1 Phase separated (incompatible) Phase separated (incompatible) Comparative Example 2 Phase separated (incompatible) Phase separated (incompatible) Comparative Example 3 Evenly distributed Phase separated (incompatible) Comparative Example 4 Evenly distributed Phase separated (incompatible) Comparative Example 5 Evenly distributed Phase separated (incompatible) Comparative Example 6 Ingredients are clumped (unsuitable) Ingredients are clumped (unsuitable) Comparative Example 7 Evenly distributed Phase separated (incompatible) Comparative Example 8 Evenly distributed Phase separated (incompatible) Comparative Example 9 Evenly distributed Phase separated (incompatible) Comparative Example 10 Ingredients are clumped (unsuitable) Ingredients are clumped (unsuitable)

Experimental Example 2: Observation of chemical stability of paste composition for companion animals

After preparing the compositions of Examples 1, 2, and 3, they were stored at 40°C for 5 months to evaluate the chemical stability of changes or decreases in content.

The content of glucosamine and lutein was analyzed by HPLC immediately after manufacturing, and the same sample was analyzed 5 months later to evaluate any change or decrease in the content, and the results are shown in Table 5.

Specifically, the HPLC analysis conditions used for the analysis are as follows.

1) Glucosamine analysis method

Device used: Shimadzu UFLC XR HPLC

Detector: UV spectrophotometer (measurement wavelength: 184 nm)

Column: Fortic C18 column, 250x4.6mm, 5 um particle size

Mobile phase: Ammonium dihydrogen phophate: Acetonitrile = 95:5

Flow rate: 0.5 ml/min

2) Lutein analysis method

Device used: Shimadzu UFLC XR HPLC

Detector: UV spectrophotometer (measurement wavelength: 447 nm)

Column: C18, 250 mm x 4.6 mm, 5 um particle size

Mobile phase: Acetonitrile:methanol:tetrahydrofuran = 50:35:15

Flow rate: 0.7 ml/min

Results of content evaluation immediately after manufacturing Results of age evaluation after 5 months of storage at 40℃ Glucosamine content Lutein content Glucosamine content Lutein content Example 1 105 ± 3.0 (fit) 99 ± 1.1 (good) 99 ± 4.5 (fit) 95 ± 3.8 (fit) Example 2 98 ± 1.8 (fit) 99 ± 2.5 (fit) 97 ± 0.7 (good fit) 97 ± 1.0 (fit) Example 3 101 ± 3.2 (fit) 97 ± 3.0 (fit) 99 ± 1.1 (good) 97 ± 2.7 (fit)

As a result of the evaluation, the chemical stability of Examples 1, 2, and 3 of the paste composition manufactured according to the present invention was evaluated, and there was no decrease in content, and the functional ingredients maintained a stable state under accelerated conditions.

Experimental Example 3: Companion Animal Preference Evaluation Test

In order to evaluate the palatability of the paste composition according to the present invention, the palatability of 5 dogs (Pomeranian 1, Pomeranian 2, Shetland Sheepdog 1, Shetland Sheepdog 2, French Bulldog) was evaluated in the presence of experts (2 mg/kg or more). The dog breeds evaluated were medium and small dogs commonly raised in Korea, and the evaluation method was the most commonly used 2 Bowl Test method, and the total test period was 5 days. The information and results of the companion animals in the test are shown in Table 6.

As a result, the palatability of the paste composition (Example 1) manufactured according to the present invention was found to be excellent in all five animals evaluated.

Experiment date Experimental information Pomeranian1 Pomeranian 2 Shetland Sheepdog1 Shetland Sheepdog 2 French Bulldog 20.11.16 <2bowl>
Left: Example 1 (10g)
Woo: My View (10g) Development products V (6ml) V (8ml) V (20ml) V (10ml) Commercially available V (5ml) 20.11.17 <2bowl>
Left: Example 1 (10g)
Woo: My View (10g) Development products V (6ml) V (8ml) V (15ml) V (20ml) V (10ml) Commercially available 20.11.18 <2bowl>
Left: Example 1 (10g)
Woo: My View (10g) Development products V (6ml) V (8ml) V (22ml) V (11ml) Commercially available V (14ml) 20.11.19 <2bowl>
Left: Example 1 (10g)
Woo: My View (10g) Development products V (7ml) V (8ml) V (20ml) V (10ml) Commercially available V (15ml) 20.11.20 <2bowl>
Left: Example 1 (10g)
Woo: My View (10g) Development products V (6ml) V (8ml) V (20ml) V (12ml) Commercially available V (14ml)

Claims (8)

Containing 1 to 5 wt% of an aqueous phase containing a water-soluble functional ingredient, 10 to 60 wt% of an oil phase containing a fat-soluble functional ingredient, a surfactant, saturated fatty oil and protein,
The surfactant is contained in an amount of 0.1 to 5 wt%, the saturated fatty oil in an amount of 5 to 35 wt%, and the protein in an amount of 10 to 40 wt%, based on the total amount of the composition.
A paste composition for pets, characterized in that the water-soluble functional ingredients include glucosamine, collagen, and water-soluble vitamins, and the fat-soluble functional ingredients include lutein and fat-soluble vitamins. delete delete delete delete delete In the first paragraph,
A paste composition for pets, characterized in that it further comprises one or more additives selected from the group consisting of sweeteners, flavoring agents and preservatives. (1) A step for preparing an oil phase mixed with vegetable oil and fat-soluble functional ingredients, and an aqueous phase mixed with water and water-soluble functional ingredients;
(2) a step of mixing saturated fatty oil into the oil phase manufactured in the above step (1);
(3) A step of mixing a surfactant into the oil phase manufactured in the above step (2);
(4) A step of mixing and dispersing vegetable protein into the oil phase manufactured in the above step (3);
(5) a step of dispersing the aqueous phase manufactured in step (1) by mixing it with the aqueous phase manufactured in step (4); and
(6) A step of homogenizing the composition manufactured in step (5) above,
A method for producing a paste composition for companion animals, characterized in that the water-soluble phase comprises 1 to 5 wt%, the oil phase comprises 10 to 60 wt%, the surfactant comprises 0.1 to 5 wt%, the saturated fatty oil comprises 5 to 35 wt%, and the protein comprises 10 to 40 wt%.

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