KR102660622B1 - Carbon dioxide concentration control system for maintaining environment of hyperbaric oxygen chamber for cell experiment - Google Patents

Abstract

A carbon dioxide concentration control system for maintaining the environment of a high-pressure oxygen chamber for cell experiments according to an embodiment of the present application includes an input unit that receives a set concentration of carbon dioxide for cell experiments, and a sensor that measures the measured concentration of carbon dioxide inside the high-pressure oxygen chamber. and a control unit that automatically controls the carbon dioxide concentration inside the hyperbaric oxygen chamber according to the set concentration and the measured concentration, wherein the control unit presents the set concentration as a target value and sets the measured concentration as a measured value. Proportional integral calculation is performed, and the proportional control valve can be controlled to automatically open and close using the control value derived through the proportional integral calculation.

Description

Carbon dioxide concentration control system for maintaining environment of hyperbaric oxygen chamber for cell experiment}

This application relates to a carbon dioxide concentration control system for maintaining the environment of a high-pressure oxygen chamber for cell experiments.

Hyperbaric oxygen therapy is a treatment that supplies 100% oxygen to the patient at a pressure higher than atmospheric pressure and delivers oxygen to the patient's tissues. Specifically, general hyperbaric oxygen therapy maintains a high-pressure state within a special capsule with increased air pressure, allowing high-purity oxygen to be inhaled, and the dissolved oxygen obtained from this increases the oxygen concentration in the human body and improves hypoxia. It refers to the treatment that is given.

In other words, it generates oxygen in an artificial environment with a pressure of 2 to 4 atmospheres, which is higher than the atmospheric pressure of 1 atmosphere, and inhales it, causing the oxygen to dissolve in the blood of the body and supplying high-purity oxygen to various parts of the human body through capillaries. It is a cure.

When high-concentration oxygen is inhaled at a pressure higher than atmospheric pressure through hyperbaric oxygen therapy, oxygen at a higher concentration than the plasma dissolved oxygen concentration at normal atmospheric pressure is delivered to the plasma, making it easier to deliver oxygen to tissues.

Therefore, it helps form a collagen matrix essential for blood vessel formation and is effective in promoting wound healing. Hyperbaric oxygen therapy is effective in treating decompression sickness, air embolism, carbon monoxide poisoning, diabetic foot, and necrotic soft tissue infection, and is widely used as a medical treatment device to treat these diseases.

In addition, it is also used for various therapeutic purposes for rapid cell activation and rapid recovery after surgery, and has the potential for a wide range of treatments regarding cell function.

If these effects of hyperbaric oxygen treatment on cells are utilized, good results can be expected in terms of future cell therapy and tissue engineering. However, compared to the hyperbaric oxygen chamber for animals, it is more important to maintain the concentration of carbon dioxide in the hyperbaric oxygen chamber for cells to maintain an environment suitable for cell culture.

The technology behind this application is disclosed in Korean Patent Publication No. 10-1896564.

The purpose of this application is to solve the problems of the prior art described above, and to provide a carbon dioxide concentration control system that can automatically control carbon dioxide inside a hyperbaric oxygen chamber for cells using PI control.

However, the technical challenges sought to be achieved by the embodiments of the present application are not limited to the technical challenges described above, and other technical challenges may exist.

As a technical means for achieving the above-described technical problem, a carbon dioxide concentration control system for maintaining the environment of a high-pressure oxygen chamber for cell experiments according to an embodiment of the present application includes an input unit that receives the set concentration of carbon dioxide for cell experiments, and the high pressure A sensor unit that measures the measured concentration of carbon dioxide inside the oxygen chamber, and a control unit that automatically controls the carbon dioxide concentration inside the hyperbaric oxygen chamber according to the set concentration and the measured concentration, wherein the control unit targets the set concentration. It is presented as a value, the measured concentration is presented as a measured value, a proportional integral operation is performed, and the proportional control valve can be controlled to automatically open and close using the control value derived through the proportional integral operation.

According to an embodiment of the present application, the carbon dioxide concentration control system further includes a carbon dioxide tank for storing carbon dioxide and an oxygen tank for storing oxygen, and the proportional control valve is a connection portion between the carbon dioxide tank and the high-pressure oxygen chamber. It may include a carbon dioxide valve provided in and an oxygen valve provided at a connection portion between the oxygen tank and the high-pressure oxygen chamber.

According to an embodiment of the present application, the control unit maintains the internal concentration when the target value and the measured value are the same, and when the target value is greater than the measured value, the control unit adjusts the control value through the proportional integral operation. obtain, open the carbon dioxide valve according to the control value, allow carbon dioxide to flow into the hyperbaric oxygen chamber, and if the target value is less than the measured value, obtain the control value through the proportional integral operation, and adjust the control value to the control value. Accordingly, the oxygen valve is opened to allow oxygen to flow into the high-pressure oxygen chamber to control the internal concentration.

According to an embodiment of the present application, the controller may repeatedly perform the automatic control of the internal concentration for a preset time, and may terminate the automatic control when the preset time is reached.

According to an embodiment of the present application, the input unit includes a display device that displays the set concentration and the measured concentration of carbon dioxide, and the display device displays a change in the open/closed state of the proportional control valve by the automatic control. can do.

According to an embodiment of the present application, the sensor unit includes an oxygen sensor and a carbon dioxide sensor inside the high-pressure oxygen chamber, and may transmit real-time concentrations of oxygen and carbon dioxide to the control unit.

According to an embodiment of the present application, a method of controlling carbon dioxide concentration for maintaining the environment of a high-pressure oxygen chamber for cell experiments includes receiving the set concentration of carbon dioxide for cell experiments, and measuring the measured concentration of carbon dioxide inside the high-pressure oxygen chamber. A step of automatically controlling the carbon dioxide concentration inside the hyperbaric oxygen chamber according to the set concentration and the measured concentration, wherein the step of automatically controlling the carbon dioxide concentration includes presenting the set concentration as a target value, and This may be a step of presenting the measured concentration as a measured value, performing the proportional integral calculation, and controlling the proportional control valve to automatically open and close using the control value derived through the proportional integral calculation.

According to an embodiment of the present application, the step of automatically controlling the carbon dioxide concentration includes performing a maintenance step of maintaining the internal concentration if the target value and the measured value are equal, and if the target value is greater than the measured value. , obtain the control value through the proportional integral operation, open the carbon dioxide valve according to the control value, and perform a carbon dioxide inflow step of introducing carbon dioxide into the hyperbaric oxygen chamber, and when the target value is smaller than the measured value. , obtaining the control value through the proportional integral operation, opening the oxygen valve according to the control value, performing an oxygen introduction step of introducing oxygen into the hyperbaric oxygen chamber, and automatically adjusting the internal concentration for a preset time. Control may be performed repeatedly, and when the preset time is reached, the automatic control may be terminated.

The above-described means of solving the problem are merely illustrative and should not be construed as intended to limit the present application. In addition to the exemplary embodiments described above, additional embodiments may be present in the drawings and detailed description of the invention.

According to the means for solving the problem of the present application described above, it is possible to provide a carbon dioxide concentration control system that can automatically control carbon dioxide inside a high-pressure oxygen chamber for cells using PI control.

However, the effects that can be obtained herein are not limited to the effects described above, and other effects may exist.

1 is a schematic block diagram of a carbon dioxide concentration control system according to an embodiment of the present application.
Figure 2 is a schematic configuration diagram of a carbon dioxide concentration control system according to an embodiment of the present application.
Figure 3 is an operation flowchart of a method for automatically controlling carbon dioxide concentration in a carbon dioxide concentration control system according to an embodiment of the present application.
Figure 4 is a schematic operational flowchart of a carbon dioxide concentration control system according to an embodiment of the present application.

Below, with reference to the attached drawings, embodiments of the present application will be described in detail so that those skilled in the art can easily implement them. However, the present application may be implemented in various different forms and is not limited to the embodiments described herein. In order to clearly explain the present application in the drawings, parts that are not related to the description are omitted, and similar parts are given similar reference numerals throughout the specification.

Throughout this specification, when a part is said to be “connected” to another part, this means not only “directly connected” but also “electrically connected” or “indirectly connected” with another element in between. "Includes cases where it is.

Throughout this specification, when a member is said to be located “on”, “above”, “at the top”, “below”, “at the bottom”, or “at the bottom” of another member, this means that a member is located on another member. This includes not only cases where they are in contact, but also cases where another member exists between two members.

Throughout the specification of the present application, when a part "includes" a certain component, this means that it may further include other components rather than excluding other components unless specifically stated to the contrary.

Hyperbaric oxygen therapy is a treatment that administers high concentration of oxygen to the patient under high pressure. It uses 100% oxygen and a pressure of at least 1.4 atm or up to 6 atm.

When hyperbaric oxygen treatment is performed, oxygen at a much higher concentration than the normal blood oxygen concentration dissolves in the blood, transporting a lot of oxygen to tissues and organs. Therefore, the therapeutic effect is achieved by supplying enough oxygen to continuously maintain basic metabolic functions even in a state where hemoglobin is completely lost due to the increased oxygen partial pressure. Because of these therapeutic effects, hyperbaric oxygen therapy is widely used for patients with divers, air and gas embolism, diabetes, and carbon monoxide poisoning.

Over the past 20 years, scientific evidence and results regarding the positive therapeutic effects of hyperbaric oxygen therapy have been accumulated and published through animal experiment results and many clinical treatment cases. Currently, hyperbaric oxygen therapy has been introduced and used in many hospitals around the world. there is.

However, although experiments on hyperbaric oxygen therapy are conducted on people using multi-person or single-person chambers, experiments on cells are not being conducted in Korea.

Therefore, the present application seeks to provide a device and method for controlling a high-pressure oxygen chamber for cell experiments.

Specifically, the carbon dioxide concentration control system according to an embodiment of the present application can maintain the carbon dioxide concentration inside the high-pressure oxygen chamber at a concentration appropriate for cell experiment or culture through opening and closing of the proportional control valve based on proportional integral control.

1 is a schematic block diagram of a carbon dioxide concentration control system according to an embodiment of the present application.

Referring to FIG. 1, the carbon dioxide concentration control system 100 may include an input unit 110, a sensor unit 120, and a control unit 130, but the configuration of the carbon dioxide concentration control system 100 is not limited thereto. .

According to one embodiment of the present application, the carbon dioxide concentration control system 100 may include an input unit 110. The input unit 110 can receive a set concentration of carbon dioxide for cell experiments.

According to an embodiment of the present application, the input unit 110 includes a display device 111 that displays the set concentration and measured concentration of carbon dioxide, and the display device 111 opens and closes the proportional control valve 160 by automatic control. Status changes can be displayed. For example, the input unit 110 may be an LCD touch screen including the display device 111, but is not limited thereto.

In addition, the input unit 110 can receive not only the set concentration of carbon dioxide but also the set pressure inside the high-pressure oxygen chamber 170, and the display device 111 can receive not only the set concentration and measured concentration of carbon dioxide, but also the set pressure inside the high-pressure oxygen chamber 170. (170) The internal set pressure and measured pressure can be displayed, but are not limited to this.

In addition, the display device 111 can display changes in the open/closed state of the proportional control valve 160 by automatic control, and more specifically, the time during which each proportional control valve 160 was open and the corresponding open/closed state. Changes in carbon dioxide concentration and oxygen pressure inside the high-pressure oxygen chamber 170 due to changes may be displayed, but are not limited to this.

According to an embodiment of the present application, the carbon dioxide concentration control system 100 may include a sensor unit 120. The sensor unit 120 can measure a specific concentration of carbon dioxide inside the high-pressure oxygen chamber 170.

According to an embodiment of the present application, the sensor unit 120 includes an oxygen sensor and a carbon dioxide sensor inside the high-pressure oxygen chamber 170, and can transmit real-time oxygen and carbon dioxide concentrations to the control unit 130.

According to an embodiment of the present application, the sensor unit 120 includes not only a carbon dioxide sensor and an oxygen sensor, but also a pressure sensor, a temperature sensor, a humidity sensor, a flow sensor, etc. for measuring the oxygen pressure in the high-pressure oxygen chamber 170. It can be included.

Additionally, according to an embodiment of the present application, the display device 111 may display output values measured in real time from the above-described sensors. That is, the display device 111 can display the concentrations of oxygen and carbon dioxide inside the high-pressure oxygen chamber 170, and can display the temperature, humidity, and oxygen pressure inside the high-pressure oxygen chamber 170. It is not limited.

Figure 2 is a schematic configuration diagram of a carbon dioxide concentration control system according to an embodiment of the present application.

Referring to FIG. 2, the carbon dioxide concentration control system 100 further includes a carbon dioxide tank 140 for storing carbon dioxide and an oxygen tank 150 for storing oxygen, and the proportional control valve 160 is a carbon dioxide tank ( It may include a carbon dioxide valve 161 provided at a connection portion between the oxygen tank 140 and the high-pressure oxygen chamber 170, and an oxygen valve 162 provided at a connection portion between the oxygen tank 150 and the high-pressure oxygen chamber 171.

According to an embodiment of the present application, carbon dioxide and oxygen stored in the carbon dioxide tank 140 and the oxygen tank 150 are stored in the high-pressure oxygen chamber 170 by opening and closing the carbon dioxide valve 161 and the oxygen valve 161, respectively. may flow into.

Additionally, the proportional control valve 160 may also be installed at the gas outlet of the high-pressure oxygen chamber 170. The gas concentration inside the high-pressure oxygen chamber 170 can be controlled by opening and closing the carbon dioxide valve 161, the oxygen valve 162, and the proportional control valve of the gas outlet.

According to an embodiment of the present application, the carbon dioxide concentration control system 100 may include a control unit 130. The control unit 130 can automatically control the carbon dioxide concentration inside the high-pressure oxygen chamber according to the set concentration and the measured concentration.

According to an embodiment of the present application, the control unit 130 presents the set concentration as the target value, presents the measured concentration as the measured value, performs proportional integral calculation, and performs proportional control using the control value derived through the proportional integral calculation. The valve 160 can be controlled to open and close automatically.

That is, the control unit 130 presents the set concentration as the target value, presents the measured concentration as the measured value, and controls the proportional control valve 160 to automatically open and close based on this, thereby automatically adjusting the carbon dioxide concentration inside the high-pressure oxygen chamber. You can control it.

Specifically, according to an embodiment of the present application, proportional integral control (PI control), which performs proportional integral calculation, is adopted and utilized as an automatic control system to control the carbon dioxide concentration inside the high-pressure oxygen chamber 170. It can be expanded to control (PID control).

The proportional integral control system measures the output value of the object to be controlled, calculates the error by comparing the output value with the target value to be reached, and sets the derived error value to an appropriate proportionality constant ( ) and constant of integration ( ) can be determined and the control value can be derived through calculation as shown in [Equation 1] and [Equation 2] below.

[Equation 1]

[Equation 2]

In [Equation 1] and [Equation 2], is the error value, is the target value, is the measurement value, and means control value.

Below, we will look at the specific control flow for the above-described automatic control, that is, proportional integral control.

Figure 3 is an operation flowchart of a method for automatically controlling carbon dioxide concentration in a carbon dioxide concentration control system according to an embodiment of the present application.

Referring to FIG. 3, the control unit 130 can maintain the internal concentration if the target value and the measured value are the same.

Specifically, the proportional integral control system of the carbon dioxide concentration control system 100 compares the target value and the measured value, and when the target value and the measured value match, the carbon dioxide concentration inside the high-pressure oxygen chamber 170 is the concentration desired by the user. Since this means the same as, the carbon dioxide valve 161 and the oxygen valve 162 can be left blocked for a time preset by the user to maintain the carbon dioxide concentration.

Referring to FIG. 3, when the target value is greater than the measured value, the control unit 130 obtains a control value through proportional integral calculation, and opens the carbon dioxide valve 161 according to the control value to release carbon dioxide into the high-pressure oxygen chamber 170. By introducing carbon dioxide into the high-pressure oxygen chamber 170, the carbon dioxide concentration can be controlled.

Specifically, as a result of comparing the target value and the measured value, if the target value is greater than the measured value, it means that the carbon dioxide concentration inside the high-pressure oxygen chamber 170 is lower than the concentration desired by the user, so control through proportional integral calculation By opening the carbon dioxide valve 161 according to the value and allowing carbon dioxide to flow from the carbon dioxide tank 140 into the high-pressure oxygen chamber 170, the carbon dioxide concentration in the high-pressure oxygen chamber 170 can be increased.

Referring to FIG. 3, when the target value is smaller than the measured value, the control unit 130 obtains a control value through proportional integral calculation, opens the oxygen valve 162 according to the control value, and supplies oxygen into the high-pressure oxygen chamber 170. By introducing it, the carbon dioxide concentration inside the high-pressure oxygen chamber 170 can be controlled.

Specifically, as a result of comparing the target value and the measured value, if the target value is smaller than the measured value, it means that the carbon dioxide concentration inside the high-pressure oxygen chamber 170 is higher than the concentration desired by the user, so control through proportional integral calculation. By opening the oxygen valve 162 according to the value and allowing oxygen to flow from the oxygen tank 150 into the high-pressure oxygen chamber 170, the carbon dioxide concentration in the high-pressure oxygen chamber 170 can be lowered.

At this time, the carbon dioxide valve 161 and the oxygen valve 162 may be opened for a certain period of time and then blocked, or may be blocked when the target value is equal to or smaller than the measured value during repetition of automatic control. However, it is not limited to this.

Additionally, referring to FIG. 3, the control unit 130 repeatedly performs proportional integral control of the carbon dioxide concentration inside the high-pressure oxygen chamber 170 for a preset time, and when the preset time is reached, performs the above-described proportional integral control. You can quit.

Specifically, when the above-described carbon dioxide inflow or oxygen inflow is performed, if the preset time is not reached, the target value and the measured value are again compared, proportional integral control is repeated, and the inside of the high pressure oxygen chamber 170 The carbon dioxide concentration can be controlled to be the same as the target value and maintained at the same concentration as the target value.

On the other hand, when the above-described carbon dioxide inflow or oxygen inflow is performed and a preset time is reached, the automatic control can be completed by terminating the above-described proportional integral control. At this time, the preset time may be the time while the cell experiment is in progress, but is not limited thereto.

For example, for culturing animal cells, the pH concentration of the cells must be maintained at about 7.3. At this time, the required concentration of carbon dioxide is 5%, and if the user sets the carbon dioxide concentration of 5%, the target value in the above-described proportional integral control may be 5%, and the current carbon dioxide inside the high-pressure oxygen chamber 170 When the concentration is 7%, the measured value in the above-described proportional integral control may be 7%. In this case, since the target value is smaller than the measured value, the carbon dioxide concentration control system 100 can automatically control the oxygen valve 162 to open and lower the carbon dioxide concentration inside the high-pressure oxygen chamber 170. It is not limited to this.

According to another embodiment of the present application, the automatic control system using the above-described proportional integral control (PI control) and proportional integral derivative control (PID control) not only controls the concentration of carbon dioxide, but also controls the concentration of oxygen in the high-pressure oxygen chamber 170. It can also be used to control pressure.

Specifically, the carbon dioxide concentration control system 100 sets the pressure value input from the user as the pressure target value and sets the pressure value input through the pressure sensor in the high-pressure oxygen chamber 170 as the pressure measurement value to automatically control the pressure. can be performed.

For example, when the pressurization section starts, if the pressure target value is greater than the pressure measurement value, the pressure is increased by introducing mixed gas into the high-pressure oxygen chamber 170, and the pressure target value is less than or equal to the pressure measurement value. In this case, the inflow of mixed gas can be prevented.

In addition, when the decompression section starts, the pressure target value and the pressure measurement value are compared, and if the pressure target value is smaller than the pressure measurement value, a control value is obtained through proportional integral calculation, and the gas inside the high pressure oxygen chamber 170 is By exhausting, the pressure can be lowered.

At this time, if the pressure target value is less than 1 atm, it enters the vacuum section. The pressure target value and the pressure measurement value are compared. If the pressure target value is less than the pressure measurement value, the vacuum function valve is opened and the high pressure oxygen chamber is opened. (170) The internal pressure can be lowered to the set pressure target value, and if the pressure target value is greater than or equal to the pressure measurement value, the vacuum function valve can be blocked.

In addition, the automatic control for maintaining the pressure may be terminated when a preset time is reached, but is not limited to this.

According to one embodiment of the present application, the high-pressure oxygen chamber 170 may include a camera (not shown) or a microscope (not shown) for monitoring cells undergoing cell experiments.

Additionally, the display device 11 can display in real time the experiment and culture status of cells captured by the above-described camera (not shown). In other words, the user can monitor the status of cells inside the high-pressure oxygen chamber 170 in real time, but is not limited to this.

Additionally, according to an embodiment of the present application, the high-pressure oxygen chamber 170 may accommodate a well plate in which a medium for cell culture is formed, and may include a shelf on which the well plate is provided.

According to an embodiment of the present application, the high-pressure oxygen chamber 170 may be provided with a water jacket (not shown) below the inside of the high-pressure oxygen chamber 170 to maintain constant internal humidity.

Additionally, the high-pressure oxygen chamber 170 may include a humidity sensor therein, and the display device may display the measured value of the humidity sensor. The user can check the humidity inside the high-pressure oxygen chamber 170 during cell experiment or culture in real time, but is not limited to this.

Compared to the hyperbaric oxygen chamber for animals, maintaining a constant temperature is more important in the hyperbaric oxygen chamber for cells. Additionally, in order to maintain the temperature of the high-pressure oxygen chamber, the temperature of the gases flowing into the chamber also needs to be maintained at the same temperature as the temperature inside the chamber.

Therefore, the carbon dioxide concentration control system 100 according to an embodiment of the present application may further include a temperature control device capable of controlling the temperature inside the high-pressure oxygen chamber 170.

Specifically, according to one embodiment of the present application, when the cells to be tested are animal cells, the temperature control device can maintain the temperature inside the high-pressure oxygen chamber 170 at 37.0 ± 0.5°, but is not limited thereto. no.

According to an embodiment of the present application, the temperature control device may include a heater, a cooler, a heating wire, and a thermostat, but is not limited thereto.

Below, we will briefly look at the operation flow of the present application based on the details described above.

Figure 4 is a schematic operational flowchart of a carbon dioxide concentration control system according to an embodiment of the present application.

The step s210 of receiving the set concentration of carbon dioxide for the cell experiment shown in FIG. 4 can be performed by the input unit 110 described above. Therefore, even if the content is omitted below, the content described with respect to the input unit 110 can be equally applied to the description of the step s210 of receiving the set concentration.

Additionally, the step s220 of measuring the concentration of carbon dioxide inside the high-pressure oxygen chamber 170 shown in FIG. 4 may be performed by the sensor unit 120 described above. Therefore, even if the content is omitted below, the content described with respect to the sensor unit 120 can be equally applied to the description of the step (s220) of measuring the measured concentration.

Additionally, the step (s230) of automatically controlling the carbon dioxide concentration inside the high-pressure oxygen chamber 170 according to the set concentration and measured concentration shown in FIG. 4 may be performed by the control unit 130 described above. Therefore, even if the content is omitted below, the content described with respect to the control unit 130 can be equally applied to the description of the step (s230) of automatically controlling the carbon dioxide concentration.

Referring to FIG. 4, in step s210, the input unit 110 may receive a set concentration of carbon dioxide for a cell experiment.

Next, in step s220, the sensor unit 120 may measure the concentration of carbon dioxide inside the high-pressure oxygen chamber 170.

Next, in step s230, the control unit 130 may automatically control the carbon dioxide concentration inside the high-pressure oxygen chamber 170 according to the set concentration and the measured concentration.

Specifically, in the step of automatically controlling the carbon dioxide concentration (S230), the set concentration is presented as the target value, the measured concentration is presented as the measured value, a proportional integral operation is performed, and the control value derived through the proportional integral operation is used. This may be a step of controlling the proportional control valve to automatically open and close.

Referring to FIG. 3, step s230 may include a maintenance step (S233), a carbon dioxide introduction step (S231), and an oxygen introduction step (S232), depending on the comparison result between the target value and the measured value.

Referring to FIG. 3, in step s233, the control unit 130 may maintain the internal concentration if the target value and the measured value are the same.

In addition, in step s231, if the target value is greater than the measured value, the control unit 130 obtains a control value through proportional integral calculation, opens the carbon dioxide valve according to the control value, and allows carbon dioxide to flow into the high-pressure oxygen chamber 170. You can.

Additionally, in step s232, if the target value is smaller than the measured value, the control unit 130 obtains a control value through the proportional integral operation and opens the oxygen valve according to the control value to introduce oxygen into the high-pressure oxygen chamber 170. You can.

Additionally, in step s230 (s231, s232, and s233), the control unit 130 may repeatedly perform automatic control of the internal concentration for a preset time, and terminate the automatic control when the preset time is reached.

In the above description, steps S210 to S230 and steps S231 to S233 may be further divided into additional steps or combined into fewer steps, depending on the implementation of the present application. Additionally, some steps may be omitted or the order between steps may be changed as needed.

The carbon dioxide concentration control method according to an embodiment of the present application may be implemented in the form of program instructions that can be executed through various computer means and recorded on a computer-readable medium. The computer-readable medium may include program instructions, data files, data structures, etc., singly or in combination. Program instructions recorded on the medium may be specially designed and constructed for the present invention or may be known and usable by those skilled in the art of computer software. Examples of computer-readable recording media include magnetic media such as hard disks, floppy disks, and magnetic tapes, optical media such as CD-ROMs and DVDs, and magnetic media such as floptical disks. -Includes optical media (magneto-optical media) and hardware devices specifically configured to store and execute program instructions, such as ROM, RAM, flash memory, etc. Examples of program instructions include machine language code, such as that produced by a compiler, as well as high-level language code that can be executed by a computer using an interpreter, etc. The hardware devices described above may be configured to operate as one or more software modules to perform the operations of the present invention, and vice versa.

The description of the present application described above is for illustrative purposes, and those skilled in the art will understand that the present application can be easily modified into other specific forms without changing its technical idea or essential features. Therefore, the embodiments described above should be understood in all respects as illustrative and not restrictive. For example, each component described as single may be implemented in a distributed manner, and similarly, components described as distributed may also be implemented in a combined form.

The scope of the present application is indicated by the claims described below rather than the detailed description above, and all changes or modified forms derived from the meaning and scope of the claims and their equivalent concepts should be construed as being included in the scope of the present application.

100: Carbon dioxide concentration control system
110: input unit
111: display device
120: sensor unit
130: control unit
140: carbon dioxide tank
150: oxygen tank
160: proportional control valve

Claims (8)

In the carbon dioxide concentration control system for maintaining the environment of a high-pressure oxygen chamber for cell experiments,
An input unit that receives the set concentration of carbon dioxide and set oxygen pressure for cell experiments;
A sensor unit that measures the concentration of carbon dioxide in the high-pressure oxygen chamber and measures the oxygen pressure in the high-pressure oxygen chamber;
a control unit that automatically controls the carbon dioxide concentration inside the hyperbaric oxygen chamber according to the set concentration and the measured concentration and the oxygen pressure inside the hyperbaric oxygen chamber according to the set oxygen pressure; and
Includes a carbon dioxide tank for storing carbon dioxide and an oxygen tank for storing oxygen,
The proportional control valve is,
It includes a carbon dioxide valve provided at a connection portion between the carbon dioxide tank and the high-pressure oxygen chamber, and an oxygen valve provided at a connection portion between the oxygen tank and the high-pressure oxygen chamber,
The control unit,
The set concentration is presented as a target value, the measured concentration is presented as a measured value, a proportional integral operation is performed, and the proportional control valve is controlled to automatically open and close using the control value derived through the proportional integral operation,
The control unit,
If the target value and the measured value are the same, maintaining the internal concentration,
When the target value is greater than the measured value, the control value is obtained through the proportional integral operation, the carbon dioxide valve is opened according to the control value, and carbon dioxide is introduced into the high-pressure oxygen chamber,
When the target value is less than the measured value, the control value is obtained through the proportional integral operation, the oxygen valve is opened according to the control value, and oxygen is introduced into the high-pressure oxygen chamber to control the internal concentration. ,
The control unit,
When the set oxygen pressure is higher than the pressure measured in the high-pressure oxygen chamber, a mixed gas is introduced into the high-pressure oxygen chamber to increase the pressure in the high-pressure oxygen chamber,
If the set oxygen pressure is lower than the pressure measured in the high-pressure oxygen chamber, the gas inside the high-pressure oxygen chamber is exhausted to reduce the pressure in the high-pressure oxygen chamber. If the set oxygen pressure is less than 1 atmosphere, By opening the vacuum function valve, the pressure inside the high-pressure oxygen chamber is lowered to enter the vacuum section,
The input unit,
A display device that displays the set concentration and the measured concentration of carbon dioxide and displays a change in the open/closed state of the proportional control valve by the automatic control,
The high-pressure oxygen chamber,
Includes a camera or microscope for photographing cells undergoing cell experiment,
The display device is,
The experimental state and culture state of cells photographed with the camera or the microscope are displayed in real time, and the interior of the high pressure oxygen chamber is determined by the time while the proportional control valve was open and the change in the open/closed state of each of the proportional control valves. A carbon dioxide concentration control system, wherein the carbon dioxide concentration control system displays changes in at least one of carbon dioxide concentration and oxygen pressure. delete delete According to paragraph 1,
The control unit,
Repeating the automatic control of the internal concentration for a preset time,
When the preset time is reached, the automatic control is terminated,
Carbon dioxide concentration control system. delete According to paragraph 1,
The sensor unit,
Including an oxygen sensor and a carbon dioxide sensor inside the high-pressure oxygen chamber,
Transmitting real-time oxygen and carbon dioxide concentrations to the control unit,
Carbon dioxide concentration control system. In the method of controlling carbon dioxide concentration to maintain the environment of a high-pressure oxygen chamber for cell experiments,
Step of inputting a set concentration of carbon dioxide and a set oxygen pressure for cell experiment;
Measuring the measured concentration of carbon dioxide inside the high-pressure oxygen chamber and measuring the oxygen pressure within the high-pressure oxygen chamber; and
Automatically controlling the carbon dioxide concentration inside the high-pressure oxygen chamber according to the set concentration and the measured concentration; and
Automatically controlling the oxygen pressure inside the high-pressure oxygen chamber according to the set oxygen pressure,
Including,
Proportional control valve is,
It includes a carbon dioxide valve provided at a connection portion between a carbon dioxide tank storing carbon dioxide and the high-pressure oxygen chamber, and an oxygen valve provided at a connection portion between an oxygen tank storing oxygen and the high-pressure oxygen chamber,
The step of automatically controlling the carbon dioxide concentration is,
Presenting the set concentration as a target value, presenting the measured concentration as a measured value, performing proportional integral calculation, and controlling the proportional control valve to automatically open and close using the control value derived through the proportional integral calculation. ,
The step of automatically controlling the carbon dioxide concentration is,
If the target value and the measured value are the same, performing a maintenance step to maintain the internal concentration,
When the target value is greater than the measured value, the control value is obtained through the proportional integral operation, the carbon dioxide valve is opened according to the control value, and a carbon dioxide inflow step is performed to introduce carbon dioxide into the high-pressure oxygen chamber,
If the target value is less than the measured value, the control value is obtained through the proportional integral operation, the oxygen valve is opened according to the control value, and oxygen is introduced into the high-pressure oxygen chamber. ,
The step of automatically controlling the oxygen pressure is,
When the set oxygen pressure is higher than the pressure measured in the high-pressure oxygen chamber, a mixed gas is introduced into the high-pressure oxygen chamber to increase the pressure in the high-pressure oxygen chamber,
If the set oxygen pressure is lower than the pressure measured in the high-pressure oxygen chamber, the gas inside the high-pressure oxygen chamber is exhausted to reduce the pressure in the high-pressure oxygen chamber, but if the set oxygen pressure is less than 1 atmosphere, By opening the vacuum function valve, the pressure inside the high-pressure oxygen chamber is lowered to enter the vacuum section,
The high-pressure oxygen chamber,
A display device that displays the set concentration and the measured concentration of carbon dioxide and displays a change in the open/closed state of the proportional control valve by the automatic control,
Includes a camera or microscope to photograph cells undergoing cell experiment,
The display device is,
Displays the experimental state and culture state of cells photographed with the camera or the microscope in real time, and displays the inside of the high-pressure oxygen chamber by the time while the proportional control valve was open and the change in the open/closed state of each of the proportional control valves. A method of controlling carbon dioxide concentration, wherein a change in at least one of carbon dioxide concentration and oxygen pressure is displayed.
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