KR102660008B1 - Liquid Suction Device for Lateral Flow Assay Using Movable Button with Absorbent Paper - Google Patents
Liquid Suction Device for Lateral Flow Assay Using Movable Button with Absorbent Paper Download PDFInfo
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- KR102660008B1 KR102660008B1 KR1020210133785A KR20210133785A KR102660008B1 KR 102660008 B1 KR102660008 B1 KR 102660008B1 KR 1020210133785 A KR1020210133785 A KR 1020210133785A KR 20210133785 A KR20210133785 A KR 20210133785A KR 102660008 B1 KR102660008 B1 KR 102660008B1
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- movable button
- blood
- solution
- buffer
- suction device
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- 230000002745 absorbent Effects 0.000 title claims abstract description 17
- 239000002250 absorbent Substances 0.000 title claims abstract description 17
- 239000007788 liquid Substances 0.000 title claims abstract description 16
- 238000003556 assay Methods 0.000 title description 2
- 239000008280 blood Substances 0.000 claims abstract description 19
- 210000004369 blood Anatomy 0.000 claims abstract description 19
- 239000000872 buffer Substances 0.000 claims abstract description 19
- 239000000758 substrate Substances 0.000 claims abstract description 16
- 238000005206 flow analysis Methods 0.000 claims abstract description 14
- 238000010521 absorption reaction Methods 0.000 claims description 6
- 239000000243 solution Substances 0.000 description 10
- 239000012528 membrane Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0663—Whole sensors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/069—Absorbents; Gels to retain a fluid
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0887—Laminated structure
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
본 발명은 이동식 버튼과 흡습지를 체결을 통한 측방유동분석 액체 흡입장치에 관한 것으로, LFA에 적용하기 위한 버퍼, 혈액, 용액을 투입하는 투입구와 이동식 버튼을 수동으로 슬라이딩할 수 있도록 형성한 홈을 포함하는 상판; 상기 상판과 결합하여 기판을 지지하는 하판; 상기 상판과 하판 사이에 위치하며, 상면 일측에 버퍼, 혈액, 용액을 분석하기 위한 전기화학센서를 포함하는 기판; 및 상기 상판의 홈을 따라 슬라이딩되는 이동식 버튼;을 포함하는 것을 특징으로 한다.The present invention relates to a liquid suction device for lateral flow analysis by fastening a movable button and moisture absorbent paper, and includes an inlet for inserting buffer, blood, and solution for application to LFA, and a groove formed to enable manual sliding of the movable button. top plate; A lower plate coupled to the upper plate to support the substrate; A substrate located between the upper plate and the lower plate and including an electrochemical sensor for analyzing buffer, blood, and solution on one side of the upper surface; and a movable button that slides along the groove of the top plate.
Description
본 발명은 이동식 버튼과 흡습지를 체결을 통한 측방유동분석 액체 흡입장치에 관한 것으로, 더욱 상세하게는 흡습지를 체결한 이동식 버튼을 슬라이딩을 통해 센서 표면의 용액 흡수를 조절할 수 있는 측방유동분석 액체 흡입장치에 관한 것이다.The present invention relates to a lateral flow analysis liquid suction device by fastening a movable button and a hygroscopic paper. More specifically, a lateral flow analysis liquid suction device that can control the absorption of solution on the sensor surface by sliding a movable button fastened to the hygroscopic paper. It's about.
측방유동분석(lateral flow assay; LFA)은 간단한 현장 진단법 중 하나이다. 이에 단백질, 항생제, 유독 물질 등을 검출하기 위한 방법으로 응용되어 왔다. Lateral flow assay (LFA) is one of the simple point-of-care diagnostic methods. Accordingly, it has been applied as a method to detect proteins, antibiotics, toxic substances, etc.
이러한, LFA는 특이적으로 결합할 수 있는 생물학적 또는 화학적 물질을 나이트로셀룰로오스 멤브레인(Nitrocellulose membrane)에 미리 고정시킴으로써, 액체 시료가 모세관 현상에 의해 흘러가면서 테스트와 컨트롤 라인(Control line)에 검출 신호를 형성하여 목표 물질을 검출하는 방법으로 알려져 있다.This LFA fixes biological or chemical substances that can specifically bind to a nitrocellulose membrane in advance, thereby sending a detection signal to the test and control lines as the liquid sample flows through capillary action. It is known as a method of detecting a target substance by forming it.
그러나, 기존 LFA는 용액이 멤브레인을 따라 모세관력에 의하여 스스로 이동하므로 추가적인 펌프 및 밸브 등과 같은 장치 없이 이용가능한 장점이 있지만, 용액 제어가 힘들기 때문에 반응시간 조절 및 잔여물 제거 등에 제한적이며, 이로 인해 민감도가 낮은 문제점이 있다. However, the existing LFA has the advantage of being able to be used without additional devices such as pumps and valves because the solution moves by itself through capillary force along the membrane. There is a problem with low sensitivity.
상기와 같은 문제점을 해결하기 위한 본 발명의 목적은 용액 제어를 통하여 LFA의 짧은 반응시간을 길게 만들어 주거나, 센서 표면에 존재하는 잔여물을 효과적으로 제거함으로써 센서 민감도를 증가시킬 수 있는 이동식 버튼과 흡습지를 체결을 통한 측방유동분석 액체 흡입장치를 제공하는 데 있다. The purpose of the present invention to solve the above problems is to provide a movable button and absorbent paper that can increase sensor sensitivity by increasing the short response time of the LFA through solution control or effectively removing residues present on the sensor surface. The purpose is to provide a lateral flow analysis liquid suction device through fastening.
상기와 같은 목적을 달성하기 위한 본 발명의 이동식 버튼과 흡습지를 체결을 통한 측방유동분석 액체 흡입장치는 LFA에 적용하기 위한 버퍼, 혈액, 용액을 투입하는 투입구와 이동식 버튼을 수동으로 슬라이딩할 수 있도록 형성한 홈을 포함하는 상판; 상기 상판과 결합하여 기판을 지지하는 하판; 상기 상판과 하판 사이에 위치하며, 상면 일측에 버퍼, 혈액, 용액을 분석하기 위한 전기화학센서를 포함하는 기판; 및 상기 상판의 홈을 따라 슬라이딩되는 이동식 버튼;을 포함하는 것을 특징으로 한다.To achieve the above object, the lateral flow analysis liquid suction device of the present invention by fastening the movable button and the moisture absorbent paper allows manually sliding the inlet and movable button for inserting buffer, blood, and solution for application to LFA. A top plate including a formed groove; A lower plate coupled to the upper plate to support the substrate; A substrate located between the upper plate and the lower plate and including an electrochemical sensor for analyzing buffer, blood, and solution on one side of the upper surface; and a movable button that slides along the groove of the top plate.
상기 전기화학센서는 기판 자체에 일체화되는 것을 특징으로 한다.The electrochemical sensor is characterized by being integrated into the substrate itself.
상기 이동식 버튼의 하면에는 버퍼, 혈액, 용액을 흡습하는 흡습지가 체결되어, 상기 상판의 홈을 따라 이동식 버튼을 슬라이딩함으로써 전기화학센서 표면의 버퍼, 혈액, 용액을 흡습하는 것을 특징으로 한다.A hygroscopic paper that absorbs the buffer, blood, and solution is fastened to the lower surface of the movable button, and the movable button is slid along the groove of the upper plate to absorb the buffer, blood, and solution on the surface of the electrochemical sensor.
상기 이동식 버튼의 하면에 체결된 흡습지를 상판의 홈을 따라 슬라이딩을 조절하여 전기화학센서 표면의 버퍼, 혈액, 용액 흡수를 조절하는 것을 특징으로 한다.The absorbent paper fastened to the lower surface of the movable button is controlled to slide along the groove of the upper plate to control the absorption of buffer, blood, and solution on the surface of the electrochemical sensor.
이상과 같이, 본 발명에 따르면 흡습지와 이동 가능한 버튼의 체결을 통하여 기존 LFA의 상시 흡습 기능을 개선하여 센서 표면 용액의 흡습 유무의 능동적 조절이 가능하다. As described above, according to the present invention, the constant moisture absorption function of the existing LFA is improved through fastening of the moisture absorbent paper and the movable button, making it possible to actively control the presence or absence of moisture absorption of the sensor surface solution.
도 1은 본 발명에 따른 이동식 버튼과 흡습지 체결을 통한 측방유동분석 액체 흡입장치를 나타낸 분해 사시도이다.
도 2는 본 발명에 따른 이동식 버튼과 흡습지 체결을 통한 측방유동분석 액체 흡입장치를 나타낸 사진이다.Figure 1 is an exploded perspective view showing a lateral flow analysis liquid suction device through fastening a movable button and moisture absorbent paper according to the present invention.
Figure 2 is a photograph showing a lateral flow analysis liquid suction device through fastening a movable button and moisture absorbent paper according to the present invention.
아래에서는 첨부한 도면을 참조하여 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자가 용이하게 실시할 수 있도록 본 발명의 실시 예를 상세히 설명한다. 그러나 본 발명은 여러 가지 상이한 형태로 구현될 수 있으며 여기에서 설명하는 실시 예에 한정되지 않는다. Below, with reference to the attached drawings, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement the present invention. However, the present invention may be implemented in many different forms and is not limited to the embodiments described herein.
그러면 본 발명에 따른 이동식 버튼과 흡습지 체결을 통한 측방유동분석 액체 흡입장치의 바람직한 실시예에 대하여 상세히 설명하기로 한다.Then, a preferred embodiment of the lateral flow analysis liquid suction device by fastening the movable button and the moisture absorbent paper according to the present invention will be described in detail.
도 1은 본 발명에 따른 이동식 버튼과 흡습지 체결을 통한 측방유동분석 액체 흡입장치를 나타낸 분해 사시도이며, 도 2는 본 발명에 따른 이동식 버튼과 흡습지 체결을 통한 측방유동분석 액체 흡입장치를 나타낸 사진이다.Figure 1 is an exploded perspective view showing a lateral flow analysis liquid suction device through fastening a movable button and moisture absorbent paper according to the present invention, and Figure 2 is a photograph showing a lateral flow analysis liquid suction device through fastening a movable button and moisture absorbent paper according to the present invention. .
도 1 및 도 2를 참조하면, 본 발명의 이동식 버튼과 흡습지 체결을 통한 측방유동분석 액체 흡입장치는 상판(10), 하판(20), 및 기판(30)을 포함하여 구성된다. Referring to Figures 1 and 2, the liquid suction device for lateral flow analysis by fastening a movable button and moisture absorbent paper of the present invention is comprised of an upper plate (10), a lower plate (20), and a substrate (30).
상기 상판(10)은 LFA에 적용하기 위한 버퍼, 혈액, 용액을 투입하는 투입구(11)와, 이동식 버튼(40)을 수동으로 슬라이딩할 수 있도록 형성한 홈(12)을 포함할 수 있다. The upper plate 10 may include an inlet 11 through which buffers, blood, and solutions for application to the LFA are introduced, and a groove 12 formed so that the movable button 40 can be manually slid.
상기 하판(20)은 상판(10)과 결합하여 기판(30)을 지지할 수 있다. The lower plate 20 may be combined with the upper plate 10 to support the substrate 30.
상기 기판(30)은 상판(10)과 하판(20) 사이에 위치하며, 기판(30)의 상면 일측에는 버퍼, 혈액, 용액을 분석하기 위한 전기화학센서(31)를 포함할 수 있다. 여기서, 전기화학센서(31)는 기판(30) 자체에 일체화될 수 있다. The substrate 30 is located between the upper plate 10 and the lower plate 20, and may include an electrochemical sensor 31 for analyzing buffer, blood, and solution on one side of the upper surface of the substrate 30. Here, the electrochemical sensor 31 may be integrated into the substrate 30 itself.
상기 이동식 버튼(40) 하면에는 버퍼, 혈액, 용액을 흡습하는 흡습지(41)가 체결되어 상판(10)의 홈(12)을 따라 이동식 버튼(40)을 슬라이딩함으로써 전기화학센서(31) 표면의 버퍼, 혈액, 용액을 흡습할 수 있다. A moisture absorbing paper 41 that absorbs buffer, blood, and solution is fastened to the bottom of the movable button 40, and the surface of the electrochemical sensor 31 is removed by sliding the movable button 40 along the groove 12 of the upper plate 10. Can absorb moisture from buffers, blood, and solutions.
즉, 상기 이동식 버튼(40) 하면에 체결된 흡습지(41)를 상판(10)의 홈(12)을 따라 슬라이딩을 조절하여 전기화학센서(31) 표면의 버퍼, 혈액, 용액 흡수를 조절할 수 있다. In other words, the absorbent paper 41 fastened to the bottom of the movable button 40 can be adjusted to slide along the groove 12 of the upper plate 10 to control the absorption of buffer, blood, and solution on the surface of the electrochemical sensor 31. .
여기서, 상기 이동식 버튼(40)은 LFA와 쉽게 체결이 가능하며 손가락으로 조절이 가능한 수동식 버튼이다.Here, the movable button 40 is a manual button that can be easily connected to the LFA and can be controlled with a finger.
이상에서 본 발명의 실시예에 대하여 상세하게 설명하였지만 본 발명의 권리범위는 이에 한정되는 것은 아니고 다음의 청구범위에서 정의하고 있는 본 발명의 기본 개념을 이용한 당업자의 여러 변형 및 개량 형태 또한 본 발명의 권리범위에 속하는 것이다.Although the embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements made by those skilled in the art using the basic concept of the present invention defined in the following claims are also possible. It falls within the scope of rights.
10: 상판 11: 투입구
12: 홈 20: 하판
30: 기판 31: 센서
40: 이동식 버튼 41: 흡습지 10: Top plate 11: Inlet
12: Home 20: Bottom plate
30: substrate 31: sensor
40: Movable button 41: Absorbent paper
Claims (4)
상기 상판과 결합하여 기판을 지지하는 하판;
상기 상판과 하판 사이에 위치하며, 상면 일측에 버퍼, 혈액, 용액을 분석하기 위한 전기화학센서를 포함하는 기판; 및
상기 상판의 홈을 따라 슬라이딩되는 이동식 버튼;을 포함하고,
상기 이동식 버튼의 하면에는 버퍼, 혈액, 용액을 흡습하는 흡습지가 체결되어, 상기 상판의 홈을 따라 상기 이동식 버튼을 슬라이딩함으로써 상기 전기화학센서 표면의 버퍼, 혈액, 용액을 흡습하는 것을 특징으로 하는 이동식 버튼과 흡습지를 체결을 통한 측방유동분석 액체 흡입장치.A top plate including an inlet for injecting buffer, blood, and solution for application to the LFA, and a groove formed to allow manual sliding of a movable button;
A lower plate coupled to the upper plate to support the substrate;
A substrate located between the upper plate and the lower plate and including an electrochemical sensor for analyzing buffer, blood, and solution on one side of the upper surface; and
Includes a movable button that slides along the groove of the top plate,
A hygroscopic paper that absorbs buffer, blood, and solution is fastened to the lower surface of the movable button, and the movable button absorbs the buffer, blood, and solution on the surface of the electrochemical sensor by sliding the movable button along the groove of the upper plate. Lateral flow analysis liquid suction device by fastening a button and moisture absorbent paper.
상기 전기화학센서는 기판 자체에 일체화되는 것을 특징으로 하는 이동식 버튼과 흡습지를 체결을 통한 측방유동분석 액체 흡입장치.According to paragraph 1,
The electrochemical sensor is a liquid suction device for lateral flow analysis by fastening a movable button and moisture absorbent paper, characterized in that the electrochemical sensor is integrated into the substrate itself.
상기 상판과 결합하여 기판을 지지하는 하판;
상기 상판과 하판 사이에 위치하며, 상면 일측에 버퍼, 혈액, 용액을 분석하기 위한 전기화학센서를 포함하는 기판; 및
상기 상판의 홈을 따라 슬라이딩되는 이동식 버튼;을 포함하고,
상기 이동식 버튼의 하면에 체결된 흡습지를 상기 상판의 홈을 따라 슬라이딩을 조절하여 상기 전기화학센서 표면의 버퍼, 혈액, 용액 흡수를 조절하는 것을 특징으로 하는 이동식 버튼과 흡습지를 체결을 통한 측방유동분석 액체 흡입장치.A top plate including an inlet for injecting buffer, blood, and solution for application to the LFA, and a groove formed to allow manual sliding of a movable button;
A lower plate coupled to the upper plate to support the substrate;
A substrate located between the upper plate and the lower plate and including an electrochemical sensor for analyzing buffer, blood, and solution on one side of the upper surface; and
Includes a movable button that slides along the groove of the top plate,
Lateral flow analysis by fastening the movable button and the hygroscopic paper, characterized in that the absorbent paper fastened to the lower surface of the movable button is controlled to slide along the groove of the upper plate to control the absorption of buffer, blood, and solution on the surface of the electrochemical sensor. Liquid suction device.
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Citations (1)
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| KR101960634B1 (en) * | 2018-04-19 | 2019-07-04 | 고려대학교 세종산학협력단 | Single-channel, hybrid biosensor and sensor system including the same |
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| KR101960634B1 (en) * | 2018-04-19 | 2019-07-04 | 고려대학교 세종산학협력단 | Single-channel, hybrid biosensor and sensor system including the same |
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