KR102629108B1 - Planting Medium Antimicrobial Composition - Google Patents
Planting Medium Antimicrobial Composition Download PDFInfo
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- KR102629108B1 KR102629108B1 KR1020230116333A KR20230116333A KR102629108B1 KR 102629108 B1 KR102629108 B1 KR 102629108B1 KR 1020230116333 A KR1020230116333 A KR 1020230116333A KR 20230116333 A KR20230116333 A KR 20230116333A KR 102629108 B1 KR102629108 B1 KR 102629108B1
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- plant
- medium
- isothiazolin
- methyl
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- 230000000845 anti-microbial effect Effects 0.000 title claims abstract description 72
- 239000000203 mixture Substances 0.000 title claims abstract description 67
- 235000015097 nutrients Nutrition 0.000 claims abstract description 35
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 26
- 238000004161 plant tissue culture Methods 0.000 claims abstract description 22
- 239000002609 medium Substances 0.000 claims description 75
- QELSKZZBTMNZEB-UHFFFAOYSA-N propylparaben Chemical compound CCCOC(=O)C1=CC=C(O)C=C1 QELSKZZBTMNZEB-UHFFFAOYSA-N 0.000 claims description 58
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 claims description 52
- DHNRXBZYEKSXIM-UHFFFAOYSA-N chloromethylisothiazolinone Chemical compound CN1SC(Cl)=CC1=O DHNRXBZYEKSXIM-UHFFFAOYSA-N 0.000 claims description 32
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 claims description 29
- 239000004302 potassium sorbate Substances 0.000 claims description 29
- 229940069338 potassium sorbate Drugs 0.000 claims description 29
- 235000010241 potassium sorbate Nutrition 0.000 claims description 29
- 239000004405 propyl p-hydroxybenzoate Substances 0.000 claims description 29
- 235000010232 propyl p-hydroxybenzoate Nutrition 0.000 claims description 29
- 229960003415 propylparaben Drugs 0.000 claims description 29
- 239000004292 methyl p-hydroxybenzoate Substances 0.000 claims description 26
- 235000010270 methyl p-hydroxybenzoate Nutrition 0.000 claims description 26
- 229940100555 2-methyl-4-isothiazolin-3-one Drugs 0.000 claims description 23
- BEGLCMHJXHIJLR-UHFFFAOYSA-N methylisothiazolinone Chemical compound CN1SC=CC1=O BEGLCMHJXHIJLR-UHFFFAOYSA-N 0.000 claims description 23
- 229940100484 5-chloro-2-methyl-4-isothiazolin-3-one Drugs 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 229960002216 methylparaben Drugs 0.000 claims description 17
- 229960003191 potassium methylparaben Drugs 0.000 claims description 9
- 239000003104 tissue culture media Substances 0.000 claims description 9
- 241000123650 Botrytis cinerea Species 0.000 claims description 6
- 241000191967 Staphylococcus aureus Species 0.000 claims description 6
- 238000011109 contamination Methods 0.000 abstract description 21
- 230000001954 sterilising effect Effects 0.000 abstract description 14
- 238000000034 method Methods 0.000 abstract description 12
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 12
- 230000000813 microbial effect Effects 0.000 abstract description 7
- 230000008569 process Effects 0.000 abstract description 6
- 239000004599 antimicrobial Substances 0.000 abstract description 3
- 239000004480 active ingredient Substances 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 73
- 239000000243 solution Substances 0.000 description 32
- 244000005700 microbiome Species 0.000 description 24
- 230000000843 anti-fungal effect Effects 0.000 description 17
- 230000002195 synergetic effect Effects 0.000 description 17
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 10
- 239000003242 anti bacterial agent Substances 0.000 description 10
- 230000012010 growth Effects 0.000 description 10
- 238000002360 preparation method Methods 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 7
- 229940121375 antifungal agent Drugs 0.000 description 7
- 231100000674 Phytotoxicity Toxicity 0.000 description 6
- 239000003429 antifungal agent Substances 0.000 description 6
- 238000012790 confirmation Methods 0.000 description 6
- 229940088710 antibiotic agent Drugs 0.000 description 5
- 229940056360 penicillin g Drugs 0.000 description 5
- 229960003669 carbenicillin Drugs 0.000 description 4
- FPPNZSSZRUTDAP-UWFZAAFLSA-N carbenicillin Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)C(C(O)=O)C1=CC=CC=C1 FPPNZSSZRUTDAP-UWFZAAFLSA-N 0.000 description 4
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 4
- 229930027917 kanamycin Natural products 0.000 description 4
- 229960000318 kanamycin Drugs 0.000 description 4
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 4
- 229930182823 kanamycin A Natural products 0.000 description 4
- 230000035755 proliferation Effects 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- 241001312221 Anthurium Species 0.000 description 3
- 240000008067 Cucumis sativus Species 0.000 description 3
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 3
- 244000115658 Dahlia pinnata Species 0.000 description 3
- 235000012040 Dahlia pinnata Nutrition 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 230000003115 biocidal effect Effects 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- 231100000419 toxicity Toxicity 0.000 description 3
- 230000001988 toxicity Effects 0.000 description 3
- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 2
- JSTFROSQSCXFPA-UHFFFAOYSA-N 2-methyl-3h-1,2-thiazole Chemical compound CN1CC=CS1 JSTFROSQSCXFPA-UHFFFAOYSA-N 0.000 description 2
- 241000194108 Bacillus licheniformis Species 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 2
- 150000001447 alkali salts Chemical class 0.000 description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000006185 dispersion Substances 0.000 description 2
- 239000003349 gelling agent Substances 0.000 description 2
- 239000003617 indole-3-acetic acid Substances 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000007492 two-way ANOVA Methods 0.000 description 2
- HYZJCKYKOHLVJF-UHFFFAOYSA-N 1H-benzimidazole Chemical compound C1=CC=C2NC=NC2=C1 HYZJCKYKOHLVJF-UHFFFAOYSA-N 0.000 description 1
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 1
- WZFUQSJFWNHZHM-UHFFFAOYSA-N 2-[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperazin-1-yl]-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethanone Chemical group C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)N1CCN(CC1)CC(=O)N1CC2=C(CC1)NN=N2 WZFUQSJFWNHZHM-UHFFFAOYSA-N 0.000 description 1
- FJKROLUGYXJWQN-UHFFFAOYSA-N 4-hydroxybenzoic acid Chemical compound OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 229920002148 Gellan gum Polymers 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- KWKVAGQCDSHWFK-VNKDHWASSA-N Methyl sorbate Chemical compound COC(=O)\C=C\C=C\C KWKVAGQCDSHWFK-VNKDHWASSA-N 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000216 gellan gum Substances 0.000 description 1
- 235000010492 gellan gum Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- MGIYRDNGCNKGJU-UHFFFAOYSA-N isothiazolinone Chemical compound O=C1C=CSN1 MGIYRDNGCNKGJU-UHFFFAOYSA-N 0.000 description 1
- 239000003120 macrolide antibiotic agent Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000001375 methyl (2E,4E)-hexa-2,4-dienoate Substances 0.000 description 1
- 230000000116 mitigating effect Effects 0.000 description 1
- 150000002960 penicillins Chemical class 0.000 description 1
- 231100000208 phytotoxic Toxicity 0.000 description 1
- 230000000885 phytotoxic effect Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 150000007660 quinolones Chemical class 0.000 description 1
- 230000033458 reproduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 229940124530 sulfonamide Drugs 0.000 description 1
- 150000003456 sulfonamides Chemical class 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/08—Alkali metal chlorides; Alkaline earth metal chlorides
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Forests & Forestry (AREA)
- Ecology (AREA)
- Chemical & Material Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Botany (AREA)
- Toxicology (AREA)
- Inorganic Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
Abstract
본 발명은 식물 배지 항미생물용 조성물에 관한 것으로, 물리화학적 안정성이 높아 고온고압멸균 과정에서 유효성분이 파괴되지 않고 잔존하여 우수한 항균 효과를 나타낼 수 있을 뿐만 아니라, 식물 배지에 간편하게 첨가할 수 있어 사용 편의성이 높으므로, 식물 조직배양용 배지나 수경재배용 양액에 대한 미생물의 오염을 방지하기 위해 효과적으로 활용될 수 있다.The present invention relates to an antimicrobial composition for plant media. It has high physical and chemical stability, so the active ingredients remain without being destroyed during the high-temperature and high-pressure sterilization process, showing excellent antibacterial effects, and can be easily added to plant media, providing convenience of use. Because this is high, it can be effectively used to prevent microbial contamination of medium for plant tissue culture or nutrient solution for hydroponic cultivation.
Description
본 발명은 식물 배지 항미생물용 조성물에 관한 것이다.The present invention relates to a composition for antimicrobial use in plant media.
본 발명은 창업진흥원, "2023년도 생애최초 청년창업" 지원사업의 과제번호 20135745, "화훼 농업인과 희귀식물 가드너를 위한 식물 조직배양 기술 기반 고부가가치 화훼류 배양 키트" 과제(2023.05.01~2023.12.31)에 따른 중소벤처기업부의 지원을 받아 이루어졌다.This invention is a project number 20135745 of the Korea Entrepreneurship & Entrepreneurship Promotion Agency's "First Youth Entrepreneurship in 2023" support project, "High value-added flower culture kit based on plant tissue culture technology for flower farmers and rare plant gardeners" project (2023.05.01 ~ 2023.12.31) ) was supported by the Ministry of SMEs and Startups.
일반적으로 식물 조직의 배양에는 아가로스나 젤란검 등의 겔화제가 배양액에 첨가된 겔상 혹은 고체상의 배지, 또는 겔화제를 포함하지 않는 액체상의 배지가 이용된다. 배지는 배양 용기에 수용된 상태로 멸균 처리되고, 그 후 무균 환경 하에서 배지에 식물 조직이 치상(置床)되며, 배양실 내에서 식물 조직의 배양이 이루어지게 된다. 이와 같이 식물의 조직배양 전 배지를 멸균하는 것은 배양 용기 내에 미생물의 혼입에 의한 미생물의 번식 및 이에 따른 식물 조직의 생육 억제를 방지하기 위함이다.In general, for culturing plant tissues, a gel or solid medium to which a gelling agent such as agarose or gellan gum is added to the culture medium, or a liquid medium without a gelling agent is used. The medium is sterilized while contained in a culture vessel, and then plant tissue is placed on the medium under a sterile environment, and the plant tissue is cultured in the culture chamber. In this way, the purpose of sterilizing the medium before culturing plant tissue is to prevent the proliferation of microorganisms due to their incorporation into the culture vessel and the resulting inhibition of the growth of plant tissue.
배지 및 배양 용기의 멸균 처리를 위하여, 미세소공 필터를 이용해 여과하여 멸균하는 여과 멸균 방법 또는 오토클레이브의 고온고압 조건 하에서 멸균하는 고온고압멸균 방식이 일반적으로 수행되고 있다. 다만, 멸균 처리 후 배지 및 배양 용기를 이동시키거나 작업하는 도중 예기치 않게 공기, 도구, 작업자의 신체 등을 통하여 배양 용기 내에 미생물의 포자가 유입되거나 식물체에 잔존하는 미생물이 유입됨으로 인하여 배양 용기 내부가 미생물에 의하여 오염될 수 있다.To sterilize media and culture vessels, a filtration sterilization method that sterilizes by filtration using a micropore filter or a high-temperature and high-pressure sterilization method that sterilizes under high-temperature and high-pressure conditions in an autoclave are generally used. However, while moving or working with the medium and culture vessel after sterilization, microbial spores unexpectedly enter the culture vessel through the air, tools, or the worker's body, or microorganisms remaining in the plant enter the culture vessel, causing the inside of the culture vessel to become damaged. May be contaminated by microorganisms.
따라서, 관행적으로 미생물의 오염을 방지하기 위하여 페니실린 G, 스트렙토마이신 등의 항생제 및/또는 벤지미다졸 등의 항진균제를 배지 제조 시 첨가하고 멸균 과정을 수행하게 되는데, 여과 멸균 방식은 수행 과정이 매우 번거롭고, 고온고압멸균 방식은 항생제의 분자 구조를 파괴하여 항균 효력을 저감시킬 수 있어 문제된다. 또한, 항생제는 식물 독성(phytotoxicity)을 나타내므로, 배양 효율이 낮아진다는 단점이 존재한다.Therefore, in order to prevent microbial contamination, antibiotics such as penicillin G and streptomycin and/or antifungal agents such as benzimidazole are customarily added when preparing the medium and the sterilization process is performed. The filtration sterilization method is a very laborious process. It is cumbersome, and the high-temperature and high-pressure sterilization method is problematic because it can destroy the molecular structure of antibiotics and reduce their antibacterial effectiveness. In addition, since antibiotics exhibit phytotoxicity, there is a disadvantage in that culture efficiency is lowered.
한편, 식물 생산 과정에서 식물 조직배양 외에도 대표적으로 배지가 사용되는 경우로는 수경재배가 있는데, 폐쇄되고 고립된 환경에서 양액을 사용하여 식물의 재배가 이루어지는 수경재배의 특성상, 양액의 오염은 양액재배에서 큰 문제로 작용한다. 따라서, 일반적으로 자외선(UV)이나 오존 발생기 등을 이용하여 양액을 살균하거나 양액을 지속적으로 순환시켜 양액에서의 미생물 번식을 억제하는 등의 방식으로 양액 내 미생물의 증식을 억제하고 있다. 다만, 이와 같은 방식은 대규모 시설에 대한 투자가 필요하며, 자외선 살균법과 같은 일부 방법의 경우에는 충분한 살균 효과를 나타내지 못하고 있는 실정이다.Meanwhile, in the plant production process, in addition to plant tissue culture, a representative case in which a medium is used is hydroponic cultivation. Due to the nature of hydroponic cultivation, in which plants are cultivated using nutrient solution in a closed and isolated environment, contamination of the nutrient solution can occur during nutrient solution cultivation. acts as a big problem. Therefore, the growth of microorganisms in the nutrient solution is generally suppressed by sterilizing the nutrient solution using ultraviolet rays (UV) or an ozone generator, or by continuously circulating the nutrient solution to suppress the growth of microorganisms in the nutrient solution. However, this method requires investment in large-scale facilities, and some methods, such as ultraviolet sterilization, do not show sufficient sterilization effect.
이에, 본 발명자들은 식물 배지에 간편하게 첨가하여 쉽게 사용할 수 있으면서도 식물 배지 내 미생물 증식 억제 효과가 우수한 항미생물용 조성물을 개발하기 위한 연구를 수행하여 본 발명을 완성하였다.Accordingly, the present inventors conducted research to develop an antimicrobial composition that can be easily used by simply adding to a plant medium and has an excellent effect of inhibiting the growth of microorganisms in the plant medium, and completed the present invention.
본 발명의 하나의 목적은 5-클로로-2-메틸-4-이소티아졸린-3-온(5-chloro-2-methyl-4-isothiazolin-3-one), 2-메틸-4-이소티아졸린-3-온(2-methyl-4-isothiazolin-3-one), 소브산칼륨(potassium sorbate), 메틸파라벤(methylparaben) 및 프로필파라벤(propylparaben)을 포함하는 식물 배지 항미생물용 조성물을 제공하는 것이다.One object of the present invention is 5-chloro-2-methyl-4-isothiazolin-3-one, 2-methyl-4-isothiazolin-3-one Providing a composition for antimicrobial plant media containing 2-methyl-4-isothiazolin-3-one, potassium sorbate, methylparaben and propylparaben. will be.
본 발명의 일 양상은 5-클로로-2-메틸-4-이소티아졸린-3-온(5-chloro-2-methyl-4-isothiazolin-3-one), 2-메틸-4-이소티아졸린-3-온(2-methyl-4-isothiazolin-3-one), 소브산칼륨(potassium sorbate), 메틸파라벤(methylparaben) 및 프로필파라벤(propylparaben)을 포함하는 식물 배지 항미생물용 조성물을 제공한다.One aspect of the present invention is 5-chloro-2-methyl-4-isothiazolin-3-one, 2-methyl-4-isothiazolin Provided is a plant medium antimicrobial composition containing -3-one (2-methyl-4-isothiazolin-3-one), potassium sorbate, methylparaben, and propylparaben.
본 발명에서 사용되는 용어, "식물 배지"는 식물 생산시 사용되는 배지를 말하며, 식물 조직 또는 캘러스(Callus)의 증식, 생장 및 분화 유도 등에 적용되는 조직배양 배지, 식물 개체가 증식, 생장 및 생식 활동을 하는데 필요한 수분 및 양분 등의 환경을 식물의 뿌리를 매개하여 제공하는 액상 또는 고상의 배지, 및 식물의 뿌리가 물리적으로 지지할 수 있도록 기계적 강도를 지원하는 배지를 포함한다. 본 발명의 식물 배지는 예를 들어, 식물 조직배양용 배지 및 식물 수경재배용 양액일 수 있다.As used in the present invention, the term "plant medium" refers to a medium used during plant production, a tissue culture medium applied to the proliferation, growth and differentiation induction of plant tissue or callus, and the proliferation, growth and reproduction of plant individuals. It includes a liquid or solid medium that provides an environment such as moisture and nutrients necessary for activity through the plant roots, and a medium that supports mechanical strength so that the plant roots can physically support it. The plant medium of the present invention may be, for example, a medium for plant tissue culture and a nutrient solution for hydroponic plant cultivation.
본 발명에 있어서, "항미생물" 또는 "항미생물 활성"이란, 세균이나 곰팡이와 같은 미생물에 대해 저항하는 성질을 의미하며, 보다 상세하게는 항생물질 등이 미생물의 성장 또는 증식을 억제하는 특성을 의미한다.In the present invention, "antimicrobial" or "antimicrobial activity" refers to the property of resisting microorganisms such as bacteria and mold, and more specifically, the property of antibiotics, etc. to inhibit the growth or proliferation of microorganisms. it means.
본 발명에 있어서, "항미생물용 조성물"은 세균이나 곰팡이와 같은 미생물의 생육을 저해하는 활성을 가진 조성물을 의미한다. 본 발명에 있어서, 상기 항미생물용 조성물은 구성요소인 5-클로로-2-메틸-4-이소티아졸린-3-온 및 2-메틸-4-이소티아졸린-3-온을 주원료로 하여 세균이나 곰팡이와 같은 미생물의 생육을 저해하는 활성을 가진 조성물을 의미한다.In the present invention, “antimicrobial composition” refers to a composition that has the activity of inhibiting the growth of microorganisms such as bacteria and mold. In the present invention, the antimicrobial composition is mainly composed of the components 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one, and is used to kill bacteria. It refers to a composition that has the activity of inhibiting the growth of microorganisms such as mold or mold.
건전한 식물체는 일반적으로 내부에 미생물이 존재하지 않지만 이병된 것이나 식물체의 구조상 뿌리나 지상부의 상처를 통하여 감염될 수 있다. 접종되는 조직 속에 단 하나의 세균이라도 감염되어 있으면 배양 중에 오염이 발생할 가능성이 있다. 식물체에서 내부감염은 흔히 간상세균에 의한 것으로 특히 Bacillus licheniformis 나 Bacillus subtilis에 의한 감염이 많으며, 이들의 포자는 열, 건조, 저온 등의 불량환경과 자외선 등에도 견딜 수 있다.Healthy plants generally do not have microorganisms inside, but diseased plants can be infected through wounds in the roots or above-ground parts due to the structure of the plant. If even a single bacterium is infected in the tissue being inoculated, contamination may occur during culture. Internal infections in plants are often caused by rod bacteria, especially Bacillus licheniformis and Bacillus subtilis , and their spores can withstand adverse environments such as heat, dryness, and low temperature, as well as ultraviolet rays.
본 발명의 항미생물용 조성물은 Bacillus licheniformis 및 Bacillus subtilis와 같은 식물 내부 감염균에 대해서도 항균효과가 우수하므로, 식물 내부 감염균에 의한 멸균 배지의 오염을 효과적으로 방지할 수 있다. 그 외에도 본 발명의 항미생물용 조성물은 황색포도상구균(Staphylococcus aureus) 및 잿빛 곰팡이병을 유발하는 진균인 보트리티스 시네레아(Botrytis cinerea)와 같은 식물 배지에서 문제되는 미생물에 대해서도 항균 활성이 우수하므로, 다양한 외부 미생물에 의한 멸균 배지의 오염을 효과적으로 방지할 수 있다.The antimicrobial composition of the present invention has an excellent antibacterial effect even against bacteria that infect plants inside plants, such as Bacillus licheniformis and Bacillus subtilis , and thus can effectively prevent contamination of the sterilization medium by bacteria that infect inside plants. In addition, the antimicrobial composition of the present invention has excellent antibacterial activity against problematic microorganisms in plant media such as Staphylococcus aureus and Botrytis cinerea , a fungus that causes gray mold disease. , contamination of the sterilization medium by various external microorganisms can be effectively prevented.
또한, 본 발명의 항미생물용 조성물은 기존의 항균제 및/또는 항진균제를 추가로 포함하여 사용될 수 있으며, 예를 들어, 페니실린 계열, 퀴놀론(quinolone) 계열, 테트라사이클린(tetracycline) 계열, 설파(sulfonamide) 계열, 마크로라이드(macrolide) 계열의 항균제/항진균제를 더 포함할 수 있으나, 이에 한정되는 것은 아니다.In addition, the antimicrobial composition of the present invention can be used by additionally containing existing antibacterial and/or antifungal agents, for example, penicillin series, quinolone series, tetracycline series, and sulfonamide. It may further include antibacterial/antifungal agents of the macrolide series, but is not limited thereto.
본 발명의 항미생물용 조성물에 기존의 항균제 및/또는 항진균제를 추가하여 사용할 경우, 서로 다른 기전의 항균/항진균 작용으로 인하여 보다 다양한 종류의 미생물에 대하여 항미생물 활성을 나타낼 수 있을 뿐만 아니라, 항미생물 활성이 더욱 증진될 수 있으며, 기존 항균/항진균제를 단독으로 사용하는 경우 대비 적은 사용량만으로도 충분한 항미생물 활성을 나타낼 수 있으므로, 기존 항균/항진균제 단독 사용에 따른 식물 독성 문제를 완화 또는 해소할 수 있다.When using the antimicrobial composition of the present invention by adding existing antibacterial and/or antifungal agents, not only can it exhibit antimicrobial activity against a wider variety of microorganisms due to the antibacterial/antifungal action of different mechanisms, but also antimicrobial activity. Activity can be further improved, and sufficient antimicrobial activity can be achieved with a small amount compared to using existing antibacterial/antifungal agents alone, thereby mitigating or resolving the problem of plant toxicity caused by the sole use of existing antibacterial/antifungal agents.
본 발명에 따른 항미생물용 조성물은 예를 들어, 직접 분사가능한 용액, 분말 및 현탁액의 형태 또는 고농축 수성, 유성 또는 다른 현탁액, 분산액, 에멀젼, 유성 분산액, 페이스트, 분진, 흩뿌림 물질 또는 과립제로 제조할 수 있으나, 이에 한정되는 것은 아니다.The antimicrobial composition according to the invention may be prepared, for example, in the form of directly sprayable solutions, powders and suspensions or as highly concentrated aqueous, oily or other suspensions, dispersions, emulsions, oily dispersions, pastes, dusts, sprinklers or granules. It can be done, but it is not limited to this.
본 발명의 일 구체예에 따르면, 5-클로로-2-메틸-4-이소티아졸린-3-온 및 2-메틸-4-이소티아졸린-3-온의 중량비는 1:1 내지 5:1일 수 있다.According to one embodiment of the present invention, the weight ratio of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one is 1:1 to 5:1. It can be.
2-메틸-4-이소티아졸린-3-온 1중량에 대하여 5-클로로-2-메틸-4-이소티아졸린-3-온이 1중량 미만으로 포함되거나, 2-메틸-4-이소티아졸린-3-온 1중량에 대하여 5-클로로-2-메틸-4-이소티아졸린-3-온이 5중량 초과로 포함될 경우, 조성물의 항균력 및 항진균력이 충분하지 않을 수 있고, 소브산칼륨, 메틸파라벤 및/또는 프로필파라벤과의 상승효과가 충분하지 않을 수 있다. 따라서, 5-클로로-2-메틸-4-이소티아졸린-3-온 및 2-메틸-4-이소티아졸린-3-온의 중량비는 1:1 내지 5:1인 것이 바람직하고, 2:1 내지 4:1인 것이 더욱 바람직하며, 3:1인 것이 가장 바람직하다.Less than 1 weight of 5-chloro-2-methyl-4-isothiazolin-3-one is contained per weight of 2-methyl-4-isothiazolin-3-one, or 2-methyl-4-isothiazolin If more than 5 weight of 5-chloro-2-methyl-4-isothiazolin-3-one is included relative to 1 weight of zolin-3-one, the antibacterial and antifungal effects of the composition may not be sufficient, and potassium sorbate , the synergistic effect with methylparaben and/or propylparaben may not be sufficient. Therefore, the weight ratio of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one is preferably 1:1 to 5:1, and 2: More preferably 1 to 4:1, most preferably 3:1.
본 발명의 일 구체예에 따르면, 상기 조성물은 물을 더 포함하고, 상기 물의 총 중량을 기준으로, 5-클로로-2-메틸-4-이소티아졸린-3-온 0.075 내지 0.1875중량%; 2-메틸-4-이소티아졸린-3-온 0.025 내지 0.0625중량%; 소브산칼륨 0.45 내지 0.60중량%; 메틸파라벤 0.30 내지 0.15중량%; 및 프로필파라벤 0.30 내지 0.15중량%를 포함할 수 있다.According to one embodiment of the present invention, the composition further includes water, and based on the total weight of the water, 0.075 to 0.1875% by weight of 5-chloro-2-methyl-4-isothiazolin-3-one; 0.025 to 0.0625% by weight of 2-methyl-4-isothiazolin-3-one; 0.45 to 0.60% by weight of potassium sorbate; 0.30 to 0.15% by weight of methylparaben; and 0.30 to 0.15% by weight of propylparaben.
물의 총 중량을 기준으로 5-클로로-2-메틸-4-이소티아졸린-3-온, 2-메틸-4-이소티아졸린-3-온 및 메틸파라벤이 각각 0.0375중량%, 0.0125중량% 및 0.35중량%일 경우, 소브산칼륨 0.40 내지 0.60중량% 및 프로필파라벤 0.35 내지 0.15중량% 범위에서는 항균력 및 항진균력에 대한 상승효과가 나타나지 않는다. 물의 총 중량을 기준으로 5-클로로-2-메틸-4-이소티아졸린-3-온, 2-메틸-4-이소티아졸린-3-온 및 메틸파라벤이 각각 0.0750중량%, 0.0250중량% 및 0.30중량%일 경우, 소브산칼륨 0.40 내지 0.55중량% 및 프로필파라벤 0.35 내지 0.20중량% 범위에서는 항균력 및 항진균력에 대한 상승효과가 나타나지 않는다.물의 총 중량을 기준으로 5-클로로-2-메틸-4-이소티아졸린-3-온, 2-메틸-4-이소티아졸린-3-온 및 메틸파라벤이 각각 0.1125중량%, 0.0375중량% 및 0.25중량%일 경우, 소브산칼륨 0.40 내지 0.45중량% 및 프로필파라벤 0.35 내지 0.30중량% 범위에서는 항균력 및 항진균력에 대한 상승효과가 나타나지 않는다. 물의 총 중량을 기준으로 5-클로로-2-메틸-4-이소티아졸린-3-온, 2-메틸-4-이소티아졸린-3-온 및 메틸파라벤이 각각 0.1500중량%, 0.0500중량% 및 0.20중량%일 경우, 소브산칼륨 0.40중량% 및 프로필파라벤 0.35중량%, 및 소브산칼륨 0.60중량% 및 프로필파라벤 0.15중량%에서는 항균력 및 항진균력에 대한 상승효과가 나타나지 않는다. 물의 총 중량을 기준으로 5-클로로-2-메틸-4-이소티아졸린-3-온, 2-메틸-4-이소티아졸린-3-온 및 메틸파라벤이 각각 0.1875중량%, 0.0625중량% 및 0.15중량%일 경우, 소브산칼륨 0.40중량% 및 프로필파라벤 0.35중량%, 및 소브산칼륨 0.55 내지 0.60중량% 및 프로필파라벤 0.20 내지 0.15중량% 범위에서는 항균력 및 항진균력에 대한 상승효과가 나타나지 않는다.Based on the total weight of water, 5-chloro-2-methyl-4-isothiazolin-3-one, 2-methyl-4-isothiazolin-3-one, and methylparaben are respectively 0.0375% by weight, 0.0125% by weight, and In the case of 0.35% by weight, there is no synergistic effect on antibacterial and antifungal activity in the range of 0.40 to 0.60% by weight of potassium sorbate and 0.35 to 0.15% by weight of propylparaben. Based on the total weight of water, 5-chloro-2-methyl-4-isothiazolin-3-one, 2-methyl-4-isothiazolin-3-one, and methylparaben are respectively 0.0750% by weight, 0.0250% by weight, and In the case of 0.30% by weight, in the range of 0.40 to 0.55% by weight of potassium sorbate and 0.35 to 0.20% by weight of propylparaben, there is no synergistic effect on antibacterial activity and antifungal activity. Based on the total weight of water, 5-chloro-2-methyl- When 4-isothiazolin-3-one, 2-methyl-4-isothiazolin-3-one and methylparaben are 0.1125%, 0.0375% and 0.25% by weight, respectively, potassium sorbate is 0.40 to 0.45% by weight. And in the range of 0.35 to 0.30% by weight of propylparaben, there is no synergistic effect on antibacterial and antifungal properties. Based on the total weight of water, 5-chloro-2-methyl-4-isothiazolin-3-one, 2-methyl-4-isothiazolin-3-one, and methylparaben are respectively 0.1500% by weight, 0.0500% by weight, and In the case of 0.20% by weight, 0.40% by weight of potassium sorbate and 0.35% by weight of propylparaben, and 0.60% by weight of potassium sorbate and 0.15% by weight of propylparaben, there is no synergistic effect on antibacterial and antifungal activities. Based on the total weight of water, 5-chloro-2-methyl-4-isothiazolin-3-one, 2-methyl-4-isothiazolin-3-one, and methylparaben are respectively 0.1875% by weight, 0.0625% by weight, and In the case of 0.15% by weight, in the range of 0.40% by weight of potassium sorbate and 0.35% by weight of propylparaben, and in the range of 0.55 to 0.60% by weight of potassium sorbate and 0.20 to 0.15% by weight of propylparaben, there is no synergistic effect on antibacterial activity and antifungal activity.
한편, 물의 총 중량을 기준으로 5-클로로-2-메틸-4-이소티아졸린-3-온, 2-메틸-4-이소티아졸린-3-온 및 메틸파라벤이 각각 0.0750중량%, 0.0250중량% 및 0.30중량%일 경우, 소브산칼륨 0.60중량% 및 프로필파라벤 0.15중량%에서 항균력 및 항진균력에 대한 상승효과가 나타난다. 물의 총 중량을 기준으로 5-클로로-2-메틸-4-이소티아졸린-3-온, 2-메틸-4-이소티아졸린-3-온 및 메틸파라벤이 각각 0.1125중량%, 0.0375중량% 및 0.25중량%일 경우, 소브산칼륨 0.50 내지 0.60중량% 및 프로필파라벤 0.25 내지 0.15중량%에서 항균력 및 항진균력에 대한 상승효과가 나타난다. 물의 총 중량을 기준으로 5-클로로-2-메틸-4-이소티아졸린-3-온, 2-메틸-4-이소티아졸린-3-온 및 메틸파라벤이 각각 0.1500중량%, 0.0500중량% 및 0.20중량%일 경우, 소브산칼륨 0.45 내지 0.55중량% 및 프로필파라벤 0.30 내지 0.20중량%에서 항균력 및 항진균력에 대한 상승효과가 나타난다. 물의 총 중량을 기준으로 5-클로로-2-메틸-4-이소티아졸린-3-온, 2-메틸-4-이소티아졸린-3-온 및 메틸파라벤이 각각 0.1875중량%, 0.0625중량% 및 0.15중량%일 경우, 소브산칼륨 0.45 내지 0.50중량% 및 프로필파라벤 0.30 내지 0.25중량%에서 항균력 및 항진균력에 대한 상승효과가 나타난다.Meanwhile, based on the total weight of water, 5-chloro-2-methyl-4-isothiazolin-3-one, 2-methyl-4-isothiazolin-3-one, and methylparaben are 0.0750% by weight and 0.0250% by weight, respectively. % and 0.30% by weight, a synergistic effect on antibacterial and antifungal activity appears at 0.60% by weight of potassium sorbate and 0.15% by weight of propylparaben. Based on the total weight of water, 5-chloro-2-methyl-4-isothiazolin-3-one, 2-methyl-4-isothiazolin-3-one, and methylparaben are respectively 0.1125% by weight, 0.0375% by weight, and In the case of 0.25% by weight, a synergistic effect on antibacterial and antifungal activity appears at 0.50 to 0.60% by weight of potassium sorbate and 0.25 to 0.15% by weight of propylparaben. Based on the total weight of water, 5-chloro-2-methyl-4-isothiazolin-3-one, 2-methyl-4-isothiazolin-3-one, and methylparaben are respectively 0.1500% by weight, 0.0500% by weight, and In the case of 0.20% by weight, a synergistic effect on antibacterial and antifungal activity appears at 0.45 to 0.55% by weight of potassium sorbate and 0.30 to 0.20% by weight of propylparaben. Based on the total weight of water, 5-chloro-2-methyl-4-isothiazolin-3-one, 2-methyl-4-isothiazolin-3-one, and methylparaben are respectively 0.1875% by weight, 0.0625% by weight, and In the case of 0.15% by weight, a synergistic effect on antibacterial and antifungal activity appears at 0.45 to 0.50% by weight of potassium sorbate and 0.30 to 0.25% by weight of propylparaben.
본 발명의 일 구체예에 따르면, 5-클로로-2-메틸-4-이소티아졸린-3-온, 2-메틸-4-이소티아졸린-3-온, 소브산칼륨, 메틸파라벤 및 프로필파라벤은 물의 총 중량을 기준으로 1.15중량%일 수 있다.According to one embodiment of the present invention, 5-chloro-2-methyl-4-isothiazolin-3-one, 2-methyl-4-isothiazolin-3-one, potassium sorbate, methylparaben and propylparaben It may be 1.15% by weight based on the total weight of water.
본 발명의 일 구체예에 따르면, 상기 식물 배지는 식물조직배양용 배지일 수 있다.According to one embodiment of the present invention, the plant medium may be a medium for plant tissue culture.
본 발명의 일 구체예에 따르면, 상기 조성물은 상기 식물조직배양용 배지 1L를 기준으로 0.5 내지 10.0㎖로 포함될 수 있다.According to one embodiment of the present invention, the composition may be included in an amount of 0.5 to 10.0 ml based on 1 L of the medium for plant tissue culture.
식물조직배양용 배지 1L를 기준으로 본 발명의 식물 배지 항미생물용 조성물이 0.5㎖ 미만으로 사용될 경우 배지 내 미생물에 대한 항미생물 활성이 충분하지 않을 수 있고, 10.0㎖ 초과로 포함될 경우 10.0㎖ 포함되는 경우와 대비하여 유의미하게 항미생물 활성이 증가하지는 않는다는 점에서, 본 발명의 항미생물 조성물은 식물조직배양용 배지 1L를 기준으로 0.5 내지 10.0㎖ 포함되는 것이 바람직하고, 1 내지 4㎖ 포함되는 것이 더욱 바람직하다.If the antimicrobial composition for plant media of the present invention is used in an amount of less than 0.5 ml based on 1 L of the medium for plant tissue culture, the antimicrobial activity against microorganisms in the medium may not be sufficient, and if it is used in an amount of more than 10.0 ml, 10.0 ml is contained. Since the antimicrobial activity does not significantly increase compared to the case, the antimicrobial composition of the present invention is preferably contained in an amount of 0.5 to 10.0 ml, and more preferably in an amount of 1 to 4 ml, based on 1 L of the medium for plant tissue culture. desirable.
본 발명의 일 구체예에 따르면, 상기 식물 배지는 수경재배용 양액일 수 있다.According to one embodiment of the present invention, the plant medium may be a nutrient solution for hydroponic cultivation.
본 발명의 일 구체예에 따르면, 상기 조성물은 상기 수경재배용 양액 총 중량을 기준으로 0.1 내지 1.5중량%로 포함될 수 있다.According to one embodiment of the present invention, the composition may be included in an amount of 0.1 to 1.5% by weight based on the total weight of the nutrient solution for hydroponic cultivation.
수경재배용 양액 총 중량을 기준으로 본 발명의 식물 배지 항미생물용 조성물이 0.1중량% 미만으로 사용될 경우 양액 내 미생물에 대한 항미생물 활성이 충분하지 않을 수 있고, 1.5중량% 초과로 포함될 경우 1.5중량% 포함되는 경우와 대비하여 유의미하게 항미생물 활성이 증가하지는 않는다는 점에서, 본 발명의 식물 배지 항미생물 조성물은 수경재배용 양액 총 중량을 기준으로 0.1 내지 1.5중량% 포함되는 것이 바람직하고, 0.15 내지 0.50중량% 포함되는 것이 더욱 바람직하다.If the antimicrobial composition for plant media of the present invention is used in an amount of less than 0.1% by weight based on the total weight of the nutrient solution for hydroponic cultivation, the antimicrobial activity against microorganisms in the nutrient solution may not be sufficient, and if it is used in an amount of more than 1.5% by weight, it may be 1.5% by weight. Since the antimicrobial activity does not significantly increase compared to the case where it is included, the plant medium antimicrobial composition of the present invention is preferably contained in an amount of 0.1 to 1.5% by weight, and 0.15 to 0.50% by weight based on the total weight of the nutrient solution for hydroponic cultivation. It is more preferable that % is included.
식물 배지 항미생물용 조성물에 따르면, 물리화학적 안정성이 높아 고온고압멸균 과정에서 유효성분이 파괴되지 않고 잔존하여 우수한 항미생물 효과를 나타낼 수 있을 뿐만 아니라, 식물 독성이 없고, 식물 배지에 간편하게 첨가할 수 있어 사용 편의성이 높으므로, 식물 조직배양용 배지나 수경재배용 양액에 대한 미생물의 오염을 방지하기 위해 효과적으로 활용될 수 있다.According to the plant medium antimicrobial composition, it has high physical and chemical stability, so the active ingredients remain without being destroyed during the high-temperature and high-pressure sterilization process, showing excellent antimicrobial effects. In addition, it is not phytotoxic and can be easily added to the plant medium. Because it is easy to use, it can be effectively used to prevent microbial contamination of media for plant tissue culture or nutrient solutions for hydroponic cultivation.
도 1은 본 발명의 일 구체예에 따른 식물 배지 항미생물용 조성물의 농도별 처리 후 14일째에 미생물 의한 식물조직배양용 배지 오염을 나타낸 사진이다.Figure 1 is a photograph showing contamination of a plant tissue culture medium by microorganisms 14 days after treatment of the plant medium antimicrobial composition at different concentrations according to an embodiment of the present invention.
이하 본 발명을 하나 이상의 실시예를 통하여 보다 상세하게 설명한다. 그러나, 이들 실시예는 본 발명을 예시적으로 설명하기 위한 것으로 본 발명의 범위가 이들 실시예에 한정되는 것은 아니다.Hereinafter, the present invention will be described in more detail through one or more examples. However, these examples are for illustrative purposes only and the scope of the present invention is not limited to these examples.
실시예 1. 5-클로로-2-메틸-4-이소티아졸린-3-온/2-메틸-4-이소티아졸린-3-온 3:1 혼합제제와 상승효과를 나타내는 소브산칼륨, 메틸파라벤 및 프로필파라벤의 혼합 비율 확인Example 1. Potassium sorbate, methyl sorbate showing synergistic effect with 5-chloro-2-methyl-4-isothiazolin-3-one/2-methyl-4-isothiazolin-3-one 3:1 mixed preparation Check the mixing ratio of paraben and propylparaben
1-1. 상승효과가 나타나는 각 성분별 혼합 비율 확인1-1. Check the mixing ratio of each ingredient that shows synergistic effects
5-클로로-2-메틸-4-이소티아졸린-3-온/2-메틸-4-이소티아졸린-3-온(MCI/MI) 3:1 혼합제제와 상승효과를 나타내는 소브산칼륨, 메틸파라벤 및 프로필파라벤의 혼합 비율을 분석하였다. Potassium sorbate, which has a synergistic effect with 5-chloro-2-methyl-4-isothiazolin-3-one/2-methyl-4-isothiazolin-3-one (MCI/MI) 3:1 mixed preparation, The mixing ratio of methylparaben and propylparaben was analyzed.
구체적으로, 식물조직배양용 배지는 Murashing&Skoog 배지 기본 염에 수크로오스를 20g/L로 첨가하고 pH는 5.7로 조정하여 배양 용기에 담아 준비하였다. 그 후, MCI/MI 3:1 혼합제제, 소브산칼륨, 메틸파라벤 및 프로필파라벤 혼합물을 물의 총 중량을 기준으로 1.15중량%가 되도록 표 1의 비율에서 선택되는 조합으로 혼합하여 식물 배지 항미생물용 조성물을 준비하였다. 그 후, 준비된 식물 배지 항미생물용 조성물을 상기 식물조직배양용 배지에 1㎖/L의 농도로 첨가하고 고온고압멸균한 다음, 배양 용기의 마개를 제거하여 공기를 통한 미생물의 유입을 허용하였으며, 배지의 오염 정도를 14일차에 육안으로 측정하여 항미생물 효과를 평가하였다. -는 오염이 육안상으로 관찰되지 않는 상태이며, +는 오염이 육안상으로 관찰된 상태이다.Specifically, the medium for plant tissue culture was prepared by adding 20 g/L of sucrose to the basic salt of Murashing & Skoog medium and adjusting the pH to 5.7 and placing it in a culture container. Afterwards, the MCI/MI 3:1 mixed preparation, potassium sorbate, methylparaben, and propylparaben mixture were mixed in a combination selected from the ratios in Table 1 to make 1.15% by weight based on the total weight of water to prepare a plant medium for antimicrobial use. A composition was prepared. Afterwards, the prepared plant medium antimicrobial composition was added to the plant tissue culture medium at a concentration of 1 mL/L and sterilized at high temperature and pressure, and then the stopper of the culture vessel was removed to allow the introduction of microorganisms through the air. The degree of contamination of the medium was visually measured on day 14 to evaluate the antimicrobial effect. - is a state in which contamination is not observed with the naked eye, and + is a state in which contamination is observed with the naked eye.
그 결과, 표 2와 같이 각 성분의 혼합 비율별 상승효과가 확인되었다.As a result, a synergistic effect was confirmed for each mixing ratio of each component, as shown in Table 2.
(중량%)Addition concentration of MCI/MI 3:1 mixed agent
(weight%)
(중량%)Methylparaben addition concentration
(weight%)
이와 같은 결과를 통하여, 물 총 중량을 기준으로 MCI/MI 3:1 혼합제제 0.10 내지 0.25중량% 범위에서 소브산칼륨 0.45 내지 0.6중량%, 메틸파라벤 0.30 내지 0.15중량% 및 프로필파라벤 0.30 내지 0.15중량%를 혼합하여 식물조직배양용 배지에 1㎖/L의 농도로 첨가할 경우, 14일 동안의 식물 조직배양 과정에서 미생물에 의한 배지의 오염을 방지하는데 상승효과를 나타낼 수 있음을 확인하였다.Through these results, based on the total weight of water, in the range of 0.10 to 0.25% by weight of the MCI/MI 3:1 mixed preparation, 0.45 to 0.6% by weight of potassium sorbate, 0.30 to 0.15% by weight of methylparaben, and 0.30 to 0.15% by weight of propylparaben. It was confirmed that when mixed and added to the plant tissue culture medium at a concentration of 1 ㎖/L, it can have a synergistic effect in preventing contamination of the medium by microorganisms during the 14-day plant tissue culture process.
1-2. 식물 배지 항미생물용 조성물의 처리 농도별 항미생물 활성 확인1-2. Confirmation of antimicrobial activity of plant medium antimicrobial composition according to treatment concentration
식물조직배양용 배지에 대한 본 발명의 식물 배지 항미생물용 조성물의 처리 농도에 따른 항미생물 활성을 분석하였다.The antimicrobial activity of the plant tissue culture medium according to the treatment concentration of the antimicrobial composition for plant medium of the present invention was analyzed.
구체적으로, 실시예 1-1에서 상승효과가 확인된 혼합 비율로써, 물 총 중량을 기준으로 MCI/MI 3:1 혼합제제, 소브산칼륨, 메틸파라벤 및 프로필파라벤이 각각 0.15중량%, 0.50중량%, 0.25중량% 및 0.25중량%로 혼합된 식물 배지 항미생물용 조성물을 식물조직배양용 배지에 0, 1, 2 또는 4㎖/L의 농도로 각각 처리한 것을 제외하면, 실시예 1-1과 동일한 방법으로 항미생물 활성을 분석하였다.Specifically, as the mixing ratio in which the synergistic effect was confirmed in Example 1-1, the MCI/MI 3:1 mixed preparation, potassium sorbate, methylparaben, and propylparaben were 0.15% by weight and 0.50% by weight, respectively, based on the total weight of water. Example 1-1, except that the plant tissue culture medium was treated with an antimicrobial composition for plant medium mixed at %, 0.25% by weight, and 0.25% by weight at a concentration of 0, 1, 2, or 4 mL/L, respectively. Antimicrobial activity was analyzed in the same manner as above.
그 결과, 무처리군에서 7일차부터 미생물에 의한 오염이 관찰된 반면, 본 발명의 식물 배지 항미생물용 조성물 처리군에서는 모든 농도에서 14일차까지 미생물에 의한 오염이 관찰되지 않았다(도 1).As a result, while contamination by microorganisms was observed from the 7th day in the untreated group, contamination by microorganisms was not observed until the 14th day at all concentrations in the group treated with the antimicrobial composition for plant medium of the present invention (Figure 1).
실시예 2. 사과 왜성대목 조직배양 시 식물 배지 항미생물용 조성물의 우수한 항미생물 활성 및 낮은 식물독성 확인Example 2. Confirmation of excellent antimicrobial activity and low phytotoxicity of plant medium antimicrobial composition during tissue culture of apple dwarf rootstock
실시예 1에서 상승효과가 나타나는 것으로 확인된 식물 배지 항미생물용 조성물의 우수한 항미생물 활성 및 낮은 식물독성을 확인하기 위하여, 사과 대목 품종인 M.26의 조직배양에 적용하였다.In order to confirm the excellent antimicrobial activity and low phytotoxicity of the plant medium antimicrobial composition, which was confirmed to have a synergistic effect in Example 1, it was applied to tissue culture of M.26, an apple rootstock variety.
구체적으로, 사과 왜성대목 품종인 M.26의 액아(Axillary bud)로부터 생장점을 채취하고, 채취한 생장점을 BAP(6-benzylamino purine) 1mg/L, IAA(Indole acetic acid) 0.5mg/L, 및 수크로스(sucrose) 30g/L를 포함하는 LS(Linsmaier and Skoog, 1965) 배지에 치상하여 90일 동안 신초를 생육시켰으며, 90일이 경과한 시점에서 신초수, 신초장, 오염율 및 고사율 등 기내(in vitro) 생육 특성을 조사하였다. 이때, 상기 식물 배지 항미생물용 조성물은 MCI/MI 3:1 혼합제제, 소브산칼륨, 메틸파라벤 및 프로필파라벤을 물의 총 중량을 기준으로 각각 0.15중량%, 0.50중량%, 0.25중량% 및 0.25중량%로 혼합하여 준비하였으며, 상기 LS 배지에 준비한 식물 배지 항미생물용 조성물을 0, 0.5, 1, 2 또는 4㎖/L의 농도로 각각 처리하였다. 또한, 비교군으로써, 페니실린 G(penicillin G), 카르베니실린(carbenicillin) 또는 카나마이신(kanamycin)을 하기 표 3의 농도로 각각 상기 배지에 처리하였다. 각 항생제별 처리 농도는 Antibiotics in Plant Tissue Culture Protocol(Merck)를 참고하여 설정하였다.Specifically, growing points were collected from the axillary buds of M.26, an apple dwarf rootstock variety, and the collected growing points were mixed with 1 mg/L of BAP (6-benzylamino purine), 0.5 mg/L of IAA (Indole acetic acid), and Shoots were grown for 90 days by planting on LS (Linsmaier and Skoog, 1965) medium containing 30 g/L of sucrose, and after 90 days, shoot number, shoot length, contamination rate, and mortality rate were measured. In vitro growth characteristics were investigated. At this time, the plant medium antimicrobial composition contains MCI/MI 3:1 mixed preparation, potassium sorbate, methylparaben, and propylparaben in amounts of 0.15% by weight, 0.50% by weight, 0.25% by weight, and 0.25% by weight, respectively, based on the total weight of water. It was prepared by mixing in %, and the plant medium antimicrobial composition prepared in the LS medium was treated at a concentration of 0, 0.5, 1, 2, or 4 mL/L. Additionally, as a comparison group, the medium was treated with penicillin G, carbenicillin, or kanamycin at the concentrations shown in Table 3 below. The treatment concentration for each antibiotic was set by referring to the Antibiotics in Plant Tissue Culture Protocol (Merck).
그 결과, 페니실린 G, 카르베니실린 또는 카나마이신 처리군 대비 본 발명의 식물 배지 항미생물용 조성물을 처리한 군에서는 유의적으로 신초수 및 신초장이 증가하였고, 고사율이 낮은 것으로 확인되었다. 또한, 미생물에 의한 오염율 역시 유의적으로 낮게 확인되었으며, 4㎖/L 처리군에서는 오염이 전혀 나타나지 않는 것으로 확인되었다. 특히, 이원분산분석에 따르면, 본 발명의 식물 배지 항미생물용 조성물과 이의 처리 농도에 따른 상호작용 효과가 신초장, 오염율 및 고사율에 유의미한 영향을 나타내는 것으로 확인되었다(표 4).As a result, compared to the group treated with penicillin G, carbenicillin or kanamycin, the group treated with the plant medium antimicrobial composition of the present invention significantly increased the number and length of shoots, and the mortality rate was confirmed to be low. In addition, the contamination rate due to microorganisms was also confirmed to be significantly low, and it was confirmed that no contamination occurred at all in the 4 ㎖/L treatment group. In particular, according to two-way analysis of variance, it was confirmed that the interaction effect depending on the plant medium antimicrobial composition of the present invention and its treatment concentration had a significant effect on shoot growth, contamination rate, and mortality rate (Table 4).
(개/절편)Shinchosu
(piece/section)
(mm)new plant
(mm)
(%)pollution rate
(%)
(%)mortality rate
(%)
항미생물용 조성물plant badge
Composition for antimicrobial use
NS,*,**Nonsignificant or significant at P≤0.05 or 0.01, respectively. a~d Mean separation within columns by Duncan's multiple range test at P=0.05.
NS,*,** Nonsignificant or significant at P≤0.05 or 0.01, respectively.
실시예 3. 다양한 식물의 조직배양 시 식물 배지 항미생물용 조성물의 낮은 식물독성 확인Example 3. Confirmation of low phytotoxicity of plant medium antimicrobial composition during tissue culture of various plants
다양한 식물의 조직배양 시에도 본 발명의 식물 배지 항미생물용 조성물이 낮은 식물독성을 나타내는지 추가적으로 분석하였다.It was further analyzed whether the plant medium antimicrobial composition of the present invention exhibits low phytotoxicity even during tissue culture of various plants.
구체적으로, MCI/MI 3:1 혼합제제, 소브산칼륨, 메틸파라벤 및 프로필파라벤을 물의 총 중량을 기준으로 각각 0.15중량%, 0.50중량%, 0.25중량% 및 0.25중량%로 혼합하여 식물 배지 항미생물용 조성물을 준비하였다. 식물조직배양용 배지는 Murashing&Skoog 배지 기본 염에 수크로오스 20g/L 및 식물생장조절제인 2,4-D 1.0mg/L를 첨가하고 pH는 5.7로 조정하여 배양 용기에 담아 준비하였다. 그 후, 식물조직배양용 배지 총 중량을 기준으로 상기 식물 배지 항미생물용 조성물을 0.05중량%부터 0.05중량% 단위로 0.50중량%까지 식물조직배양용 배지에 각각 첨가하고 고온고압멸균하였다. 잎 절편(leaf disk)은 오이, 안스리움 및 다알리아의 잎을 1cm×1cm 크기로 잘라 식물별로 20개씩 준비하였으며, 멸균된 배지에 잎 절편을 치상한 다음 절편 당 신초 줄기(shoot)의 분화율을 측정하여 식물 독성을 평가하였다. 분화율은 각 절편 당 발생한 신초의 개수를 절편 개수로 나누어 계산하였다.Specifically, MCI/MI 3:1 mixed preparation, potassium sorbate, methylparaben, and propylparaben were mixed at 0.15% by weight, 0.50% by weight, 0.25% by weight, and 0.25% by weight, respectively, based on the total weight of water, and added to the plant medium section. A composition for microorganisms was prepared. The medium for plant tissue culture was prepared by adding 20 g/L of sucrose and 1.0 mg/L of 2,4-D, a plant growth regulator, to the basic salt of Murashing & Skoog medium, adjusting the pH to 5.7, and placing it in a culture container. Thereafter, based on the total weight of the plant tissue culture medium, the plant medium antimicrobial composition was added to the plant tissue culture medium from 0.05% by weight to 0.50% by weight in 0.05% by weight units and sterilized at high temperature and pressure. Leaf disks were prepared by cutting the leaves of cucumber, anthurium, and dahlia into 1cm and evaluated plant toxicity. The differentiation rate was calculated by dividing the number of shoots generated in each segment by the number of segments.
그 결과, 모든 첨가 농도에서, 오이, 안스리움 및 다알리아의 조직배양 과정 중 식물 독성이 나타나지 않는 것으로 확인되었다(표 5).As a result, it was confirmed that no phytotoxicity occurred during tissue culture of cucumber, anthurium, and dahlia at all added concentrations (Table 5).
이와 같은 결과를 통하여, 본 발명의 식물 배지 항미생물용 조성물은 다양한 식물의 조직배양 과정 중 발생할 수 있는 배지의 미생물 오염을 식물 독성 없이 효과적으로 억제하는데 효과적으로 사용될 수 있음을 확인하였다.Through these results, it was confirmed that the antimicrobial composition for plant media of the present invention can be effectively used to effectively suppress microbial contamination of media that may occur during the tissue culture process of various plants without plant toxicity.
실시예 4. 수경재배용 양액에서 식물 배지 항미생물용 조성물의 항미생물 활성 확인Example 4. Confirmation of antimicrobial activity of plant medium antimicrobial composition in nutrient solution for hydroponic cultivation
4-1. 수경재배용 양액에서 황색포도상구균에 대한 식물 배지 항미생물용 조성물의 항균 활성 확인4-1. Confirmation of antibacterial activity of plant medium antimicrobial composition against Staphylococcus aureus in nutrient solution for hydroponic cultivation
수경재배용 양액에서 황색포도상구균(Staphylococcus aureus)에 대한 본 발명의 식물 배지 항미생물용 조성물의 첨가 농도별 항미생물 활성을 분석하였다.The antimicrobial activity of the plant medium antimicrobial composition of the present invention against Staphylococcus aureus in a nutrient solution for hydroponic cultivation was analyzed at each concentration.
구체적으로, MCI/MI 3:1 혼합제제, 소브산칼륨, 메틸파라벤 및 프로필파라벤을 물의 총 중량을 기준으로 각각 0.15중량%, 0.50중량%, 0.25중량% 및 0.25중량%로 혼합하여 식물 배지 항미생물용 조성물을 준비하였다. 수경재배용 양액은 Yamazaki 표준액 1L에 황색포도상구균을 3.2×105CFU/㎖ 농도로 접종하여 준비하였다. 접종한 직후, 수경재배용 양액 총 중량을 기준으로 상기 식물 배지 항미생물용 조성물을 0.05중량%부터 0.05중량% 단위로 0.50중량%까지 수경재배용 양액에 각각 첨가하고 25℃에서 30분 동안 방치한 후, 정균감소율 계산법에 따라 미생물 군집의 감소율을 측정하여 항균력을 평가하였다.Specifically, MCI/MI 3:1 mixed preparation, potassium sorbate, methylparaben, and propylparaben were mixed at 0.15% by weight, 0.50% by weight, 0.25% by weight, and 0.25% by weight, respectively, based on the total weight of water, and added to the plant medium section. A composition for microorganisms was prepared. Nutrient solution for hydroponic cultivation was prepared by inoculating 1L of Yamazaki standard solution with Staphylococcus aureus at a concentration of 3.2×10 5 CFU/ml. Immediately after inoculation, based on the total weight of the hydroponic nutrient solution, the plant medium antimicrobial composition is added to the hydroponic nutrient solution in an amount from 0.05% by weight to 0.50% by weight in 0.05% by weight units, and left at 25°C for 30 minutes, Antibacterial activity was evaluated by measuring the reduction rate of the microbial community according to the bacteriostatic reduction rate calculation method.
그 결과, 수경재배용 양액에 본 발명의 식물 배지 항미생물용 조성물이 0.25중량% 이상 첨가될 경우 92% 이상의 항균력을 나타내는 것으로 확인되었으며, 0.40중량% 이상 첨가될 경우 99.99%의 항균력을 나타내는 것으로 확인되었다(표 6).As a result, it was confirmed that when more than 0.25% by weight of the plant medium antimicrobial composition of the present invention was added to the nutrient solution for hydroponic cultivation, it exhibited an antibacterial activity of more than 92%, and when it was added at least 0.40% by weight, it was confirmed that it exhibited an antibacterial activity of 99.99%. (Table 6).
이와 같은 결과를 통하여, 수경재배용 양액 총 중량을 기준으로 0.40중량% 이상의 농도로 본 발명의 식물 배지 항미생물용 조성물을 첨가할 경우, 수경재배 중 황색포도상구균에 의한 양액의 오염을 방지할 수 있음을 확인하였다.Through these results, when the plant medium antimicrobial composition of the present invention is added at a concentration of 0.40% by weight or more based on the total weight of the nutrient solution for hydroponic cultivation, contamination of the nutrient solution by Staphylococcus aureus during hydroponic cultivation can be prevented. was confirmed.
4-2. 수경재배용 양액에서 보트리티스 시네레아에 대한 식물 배지 항미생물용 조성물의 항진균 활성 확인4-2. Confirmation of antifungal activity of plant medium antimicrobial composition against Botrytis cinerea in nutrient solution for hydroponic cultivation
수경재배용 양액에서 잿빛 곰팡이병을 유발하는 진균인 보트리티스 시네레아(Botrytis cinerea)에 대한 본 발명의 식물 배지 항미생물용 조성물의 첨가 농도별 항진균 활성을 분석하였다.The antifungal activity of the plant medium antimicrobial composition of the present invention against Botrytis cinerea , a fungus that causes gray mold disease in hydroponic nutrient solutions, was analyzed by concentration.
구체적으로, 수경재배용 양액은 Yamazaki 표준액 1L에 보트리티스 시네레아를 3.2×105CFU/㎖ 농도로 접종하여 준비하였다. 접종한 직후, 수경재배용 양액 총 중량을 기준으로 실시예 4-1의 식물 배지 항미생물용 조성물을 0.05중량%부터 0.05중량% 단위로 0.50중량%까지 수경재배용 양액에 각각 첨가하고 25℃에서 30분 동안 방치한 후, 정균감소율 계산법에 따라 미생물 군집의 감소율을 측정하여 항진균력을 평가하였다.Specifically, the nutrient solution for hydroponic cultivation was prepared by inoculating Botrytis cinerea at a concentration of 3.2×10 5 CFU/ml in 1L of Yamazaki standard solution. Immediately after inoculation, the plant medium antimicrobial composition of Example 4-1 was added to the hydroponic nutrient solution in an amount of 0.05% by weight to 0.50% by weight based on the total weight of the hydroponic nutrient solution, and incubated at 25°C for 30 minutes. After being left for a while, the antifungal activity was evaluated by measuring the reduction rate of the microbial community according to the bacteriostatic reduction rate calculation method.
그 결과, 수경재배용 양액에 본 발명의 식물 배지 항미생물용 조성물이 0.05중량% 이상 첨가될 경우 98.41% 이상의 항진균력을 나타내는 것으로 확인되었으며, 0.15중량% 이상 첨가될 경우 99.99%의 항진균력을 나타내는 것으로 확인되었다(표 7).As a result, it was confirmed that when more than 0.05% by weight of the plant medium antimicrobial composition of the present invention was added to the nutrient solution for hydroponic cultivation, it exhibited an antifungal activity of more than 98.41%, and when it was added more than 0.15% by weight, it showed an antifungal activity of 99.99%. confirmed (Table 7).
이와 같은 결과를 통하여, 수경재배용 양액 총 중량을 기준으로 0.15중량% 이상의 농도로 본 발명의 식물 배지 항미생물용 조성물을 첨가할 경우, 수경재배 중 보트리티스 시네레아에 의한 양액의 오염을 방지할 수 있음을 확인하였다.Through these results, when adding the plant medium antimicrobial composition of the present invention at a concentration of 0.15% by weight or more based on the total weight of the nutrient solution for hydroponic cultivation, contamination of the nutrient solution by Botrytis cinerea during hydroponic cultivation can be prevented. It was confirmed that it was possible.
이제까지 본 발명에 대하여 그 실시예들을 중심으로 살펴보았다. 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자는 본 발명이 본 발명의 본질적인 특성에서 벗어나지 않는 범위에서 변형된 형태로 구현될 수 있음을 이해할 수 있을 것이다. 그러므로 개시된 실시예들은 한정적인 관점이 아니라 설명적인 관점에서 고려되어야 한다. 본 발명의 범위는 전술한 설명이 아니라 청구범위에 나타나 있으며, 그와 동등한 범위 내에 있는 모든 차이점은 본 발명에 포함된 것으로 해석되어야 할 것이다.So far, the present invention has been examined focusing on its embodiments. A person skilled in the art to which the present invention pertains will understand that the present invention may be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative rather than a restrictive perspective. The scope of the present invention is indicated in the claims, not the foregoing description, and all differences within the equivalent scope should be construed as being included in the present invention.
Claims (9)
상기 조성물은 물, 5-클로로-2-메틸-4-이소티아졸린-3-온(5-chloro-2-methyl-4-isothiazolin-3-one), 2-메틸-4-이소티아졸린-3-온(2-methyl-4-isothiazolin-3-one), 소브산칼륨(potassium sorbate), 메틸파라벤(methylparaben) 및 프로필파라벤(propylparaben)을 포함하고,
물의 총 중량을 기준으로, 5-클로로-2-메틸-4-이소티아졸린-3-온이 0.1500 내지 0.1875중량%일 때, 2-메틸-4-이소티아졸린-3-온 0.0500 내지 0.0625중량%, 소브산칼륨 0.45 내지 0.50중량%, 메틸파라벤 0.15 내지 0.20중량%, 및 프로필파라벤 0.25 내지 0.30중량%; 또는
물의 총 중량을 기준으로, 5-클로로-2-메틸-4-이소티아졸린-3-온이 0.1125 내지 0.1500중량%일 때, 2-메틸-4-이소티아졸린-3-온 0.0375 내지 0.500중량%, 소브산칼륨 0.50 내지 0.55중량%, 메틸파라벤 0.20 내지 0.25중량%, 및 프로필파라벤 0.20 내지 0.25중량%
인 것인 식물 배지 항미생물용 조성물.
In the composition for plant medium antimicrobial,
The composition includes water, 5-chloro-2-methyl-4-isothiazolin-3-one, 2-methyl-4-isothiazolin- Contains 3-one (2-methyl-4-isothiazolin-3-one), potassium sorbate, methylparaben, and propylparaben,
0.0500 to 0.0625% by weight of 2-methyl-4-isothiazolin-3-one, when 5-chloro-2-methyl-4-isothiazolin-3-one is 0.1500 to 0.1875% by weight, based on the total weight of water. %, 0.45 to 0.50% by weight potassium sorbate, 0.15 to 0.20% by weight methylparaben, and 0.25 to 0.30% by weight propylparaben; or
0.0375 to 0.500% by weight of 2-methyl-4-isothiazolin-3-one, when 5-chloro-2-methyl-4-isothiazolin-3-one is 0.1125 to 0.1500% by weight, based on the total weight of water. %, potassium sorbate 0.50 to 0.55% by weight, methylparaben 0.20 to 0.25% by weight, and propylparaben 0.20 to 0.25% by weight.
A composition for antimicrobial plant media.
The composition for plant medium antimicrobial according to claim 1, wherein the plant medium is a medium for plant tissue culture.
The composition for plant medium antimicrobial according to claim 5, wherein the composition is contained in an amount of 0.5 to 10.0 ml based on 1 L of the plant tissue culture medium.
The composition for plant medium antimicrobial according to claim 1, wherein the plant medium is a nutrient solution for hydroponic cultivation.
The composition for antimicrobial plant media according to claim 7, wherein the composition is contained in an amount of 0.1 to 1.5% by weight based on the total weight of the nutrient solution for hydroponic cultivation.
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| KR940010899A (en) | 1992-11-27 | 1994-06-20 | 한영복 | Media additives for sterilization in plant tissue culture |
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| US20150230457A1 (en) * | 2012-09-18 | 2015-08-20 | Dow Global Technologies Llc | Microbicidal composition |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR940010899A (en) | 1992-11-27 | 1994-06-20 | 한영복 | Media additives for sterilization in plant tissue culture |
| US5750402A (en) * | 1995-06-02 | 1998-05-12 | Plant Cell Technology, Inc. | Compositions and methods to prevent microbial contamination of plant tissue culture media |
| US20040167220A1 (en) * | 2003-02-20 | 2004-08-26 | Horst R. Kenneth | Environmentally safe fungicide and bactericide formulations |
| US20150230457A1 (en) * | 2012-09-18 | 2015-08-20 | Dow Global Technologies Llc | Microbicidal composition |
| JP2016522796A (en) * | 2013-03-15 | 2016-08-04 | ジェニール バイオサーファクタント カンパニー，リミテッド ライアビリティ カンパニーＪｅｎｅｉｌ Ｂｉｏｓｕｒｆａｃｔａｎｔ Ｃｏｍｐａｎｙ，Ｌｌｃ | Antimicrobial compositions and related methods of use |
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