KR102627089B1 - Novel fungus Talaromyces gwangjuensis, Talaromyces koreana and Talaromyces teleomorha, and use thereof - Google Patents

Abstract

The present invention relates to new strains of Talaromyces gwangjuensis, Talaromyces Koreana and Talaromyces teleomorpha and their uses. Talaromyces gwangjuensis, Talaromyces Koreana and Tala according to the present invention Romayces teleomorpha is a strain that can produce various types of metabolites, and its culture or culture filtrate can inhibit or enhance specific activities that occur within cells, and has an excellent ability to produce specific compounds, so it is used in pharmaceutical, It can be used in compositions in various fields such as food and beauty.

Description

Novel fungus Talaromyces gwangjuensis, Talaromyces koreana and Talaromyces teleomorha, and use thereof}

The present invention relates to new strains of Talaromyces gwangjuensis, Talaromyces Koreana and Talaromyces teleomorpha and uses thereof.

In nature, various microorganisms such as bacteria, mold, yeast, and algae exist, and research using them continues. The fields of use of microorganisms are very diverse, and representative examples include therapeutic agents in the medical field, health functional foods in the food field, and cosmetic compositions in the beauty field.

Hundreds of thousands of species of microorganisms have been discovered and reported to date, but new strains are continuously being reported, and the discovery of new strains can lead to the invention of new uses using them. For example, Lactobacillus, a lactic acid bacterium, is a representative microorganism used in various fields and has many uses, such as being used in feed in Korea Patent No. 10-1795499 and as a treatment for obesity in Korea Patent No. 10-1736033. Varies.

Among these, fungi living in freshwater are strains that play an important role in the ecological environment and provide nutrients to other aquatic organisms. They are known to be a natural source of secondary metabolites with diverse structures and promising biological activities, and there are about 150 of them on Earth. Although it is estimated that more than 10,000 species exist, freshwater fungi, especially the Talaromyces genus, have rarely been reported in our country.

Diseases caused by fungi represent a major threat to important crops. Although applying synthetic antifungals is one of the cheapest and most effective approaches to control plant diseases, the use of synthetic antifungals not only causes disease in animals, including humans, but also has a significant impact on agricultural ecosystems. It is known. Therefore, secondary metabolites with antifungal activity are emerging as an alternative to synthetic antifungal agents to control various pathogenic molds (fungi).

The present invention is intended to expand the category of fungi living in freshwater in Korea, and sought to discover three new Talaromyces species living in freshwater in Korea and identify their functions.

European Patent Publication No. 2896681 (2015.07.22).

Yilmaz N et al., Polyphasic taxonomy of the genus Talaromyces, Studies in Mycology 78:175-341 (2014.09.22).

The present invention sought to provide new strains of Talaromyces gwangjuensis, Talaromyces Koreana, and Talaromyces teleomorpha isolated from freshwater, and compositions containing the same.

The present invention relates to Talaromyces gwangjuensis CNUFC WT19-1 (Accession number: KCTC14671BP); Talaromyces koreana CNUFC YJW2-13 (Accession number: KCTC14672BP); Alternatively, Talaromyces teleomorha CNUFC YJW2-5 (accession number: KCTC14670BP) is provided.

The present invention relates to Talaromyces gwangjuensis CNUFC WT19-1 (accession number: KCTC14671BP), Talaromyces koreana CNUFC YJW2-13 (accession number: KCTC14672BP) and Talaromyces teleomo At least one strain selected from the group consisting of Talaromyces teleomorha CNUFC YJW2-5 (Accession number: KCTC14670BP); Culture medium of the above strain; It provides a pharmaceutical composition comprising as an active ingredient at least one selected from the group consisting of culture filtrate of the above strain.

The present invention relates to Talaromyces gwangjuensis CNUFC WT19-1 (accession number: KCTC14671BP), Talaromyces koreana CNUFC YJW2-13 (accession number: KCTC14672BP) and Talaromyces teleomo At least one strain selected from the group consisting of Talaromyces teleomorha CNUFC YJW2-5 (Accession number: KCTC14670BP); Culture medium of the above strain; It provides a health functional food containing as an active ingredient at least one selected from the group consisting of culture filtrate of the above strain.

The present invention relates to Talaromyces gwangjuensis CNUFC WT19-1 (accession number: KCTC14671BP), Talaromyces koreana CNUFC YJW2-13 (accession number: KCTC14672BP) and Talaromyces teleomo At least one strain selected from the group consisting of Talaromyces teleomorha CNUFC YJW2-5 (Accession number: KCTC14670BP); Culture medium of the above strain; It provides a cosmetic composition comprising as an active ingredient at least one selected from the group consisting of culture filtrate of the above-described strain.

The present invention relates to Talaromyces gwangjuensis CNUFC WT19-1 (accession number: KCTC14671BP), Talaromyces koreana CNUFC YJW2-13 (accession number: KCTC14672BP) and Talaromyces teleomo At least one strain selected from the group consisting of Talaromyces teleomorha CNUFC YJW2-5 (Accession number: KCTC14670BP); Culture medium of the above strain; It provides an antibacterial or antifungal microbial preparation comprising as an active ingredient at least one selected from the group consisting of culture filtrate of the above strain.

In one example according to the present invention, the antifungal target of the microbial preparation is Alternaria alternata , Alternaria mali , Aspergillus fumigatus , and Aspergillus duck. Aspergillus oryzae , Basidiobolus ranarum , Botrytis cinerea, Colletotrichum coccodes , Colletotrichum gloeosporioides ), Colletotrichum orbiculare , Cylindrocarpon destructans , Fusarium oxysporum f. sp. Lycopersici ( Fusarium oxysporum f. sp. lycopersici ), Fusarium proliferatum ( Lichtheimia corymbifera ), Magnaporthe oryzae ( Magnaporthe oryzae ), Mucor zanseni ( Mucor janssenii ), Pyricularia oryzae , Rhizomucor pusillus , Rhizoctonia solani, Rhizoctonia solani var. It may be selected from the group consisting of Rhizopus stolonifer var. stolonifer .

The present invention relates to Talaromyces gwangjuensis CNUFC WT19-1 (accession number: KCTC14671BP), Talaromyces koreana CNUFC YJW2-13 (accession number: KCTC14672BP), and It provides one or more strains selected from the group consisting of Talaromyces teleomorha CNUFC YJW2-5 (accession number: KCTC14670BP).

Talaromyces gwangjuensis, Talaromyces Koreana, and Talaromyces teleomorpha according to the present invention are strains capable of producing various types of metabolites, and their culture medium or culture filtrate shows specific activities occurring within the cells. , for example, it can inhibit or improve antifungal activity, and has an excellent ability to produce specific compounds, so it can be used in compositions in various fields such as pharmacy, food, and beauty.

Figure 1 shows the results of maximum likelihood analysis (ML) through combining the ITS, BenA, CaM, and RPB2 sequence data of Talaromyces. The value is 100% ML BS, and the branch with 1 PP is the thickness of the branch. The shape was highlighted and indicated, and branches with values greater than 70% ML BS and greater than 95% PP were indicated above or below the branch. At this time , Trichocoma paradoxa CBS 788.83 was used as an outgroup.
Figure 2 shows the results of maximum likelihood analysis by combining ITS, BenA, CaM, and RPB2 sequence data for species classified in the Purpurei section of Talaromyces, where Trichocoma tenuis ( Trichocoma tenuis ) CBS 141840 was used as an outgroup.
Figure 3 shows the results of maximum likelihood analysis by combining ITS, BenA, CaM, and RPB2 sequence data for species classified in the Helici section of Talaromyces, where Trichocoma tenuis ( Trichocoma tenuis ) CBS 141840 was used as an outgroup.
Figure 4 shows the results regarding the morphology of Talaromyces gwangjuensis CNUFC WT19-1, a, e colonies on Czapeck yeast autolysate agar (CYA). b, f Malt extract agar (MEA). c Yeast extract sucrose agar (YES). d Oatmeal agar (OA). g Dichloran 18% glycerol agar (DG 18). h Creatine sucrose agar (CREA) (a - d, g, h: front view, e, f: back view). il, nq Condiophores. m, r Conidia (im: LM, nr: SEM). Scale bar: represents i - m = 20 μm, nq = 10 μm, r = 5 μm.
Figure 5 shows the results regarding the morphology of Talaromyces Koreana CNUFC YJW2-13, a, e colonies on Czapeck yeast autolysate agar (CYA). b, f Malt extract agar (MEA). c Yeast extract sucrose agar (YES). d Oatmeal agar (OA). g Dichloran 18% glycerol agar (DG 18). h Creatine sucrose agar (CREA) (a - d, g, h: front view, e, f: back view). il, nq Condiophores. m, r Conidia (im: LM, nr: SEM). Scale bars: represent i = 100 μm, j - l = 20 μm, m, n, q = 10 μm, n - p = 25 μm, r = 2 μm.
Figure 6 shows the results regarding the morphology of Talaromyces teleomorpha CNUFC YJW2-5, a, e colonies on Czapeck yeast autolysate agar (CYA). b, f Malt extract agar (MEA). c Yeast extract sucrose agar (YES). d Oatmeal agar (OA). g Dichloran 18% glycerol agar (DG 18). h Creatine sucrose agar (CREA) (a - d, g, h: front view, e, f: back view). i, j Ascomata. kp Asci and ascospores. (i, j: stereomicroscope, km: LM, np: SEM). Scale bars: represent j, j = 1 mm, k, l = 20 μm, m = 10 μm, n - p = 5 μm.
Figure 7 shows the results regarding the antifungal activity of Talaromyces gwangjuensis CNUFC WT19-1 against pathogenic molds (fungi), A) Botrytis cinerea , B) Fusarium proli Fusarium proliferatum , C) Colletotrichum gloeosporioides , D) Pyricularia oryzae , E) Alternaria alternata , F) Lychee Lichtheimia corymbifera, G) Mucor janssenii , H) Rhizomucor pusillus , I), Basidiobolus ranarum , and J) It represents Aspergillus fumigatus .
Figure 8 shows the antifungal activity of Talaromyces teleomorha CNUFC YJW2-5 (AD) and Talaromyces koreana CNUFC YJW2-13 (E) against pathogenic molds (fungi). As a result, A) Pyricularia oryzae , B) Alternaria alternata , C) Fusarium proliferatum , D) Rhizomucor pusillus ) and E) Pyricularia oryzae .

The present invention will be described in detail below with reference to the attached tables and drawings.

When drawings are described, they are provided as examples so that the idea of the present invention can be sufficiently conveyed to those skilled in the art. Accordingly, the present invention is not limited to the presented drawings and may be embodied in other forms, and the drawings may be exaggerated to clarify the spirit of the present invention.

Terms such as first, second, etc. may be used to describe various components, but the components should not be limited by the terms. The above terms are used only for the purpose of distinguishing one component from another. For example, a first component may be referred to as a second component, and similarly, the second component may be referred to as a first component without departing from the scope of the present invention.

At this time, if there is no other definition in the technical and scientific terms used, they have the meaning commonly understood by those skilled in the art to which this invention pertains, and the gist of the present invention is summarized in the following description and accompanying drawings. Descriptions of known functions and configurations that may be unnecessarily obscure are omitted.

Additionally, as used herein, the singular forms “a,” “an,” and “the” are intended to also include the plural forms, unless the context clearly dictates otherwise.

In addition, units used without special mention in the specification of the present invention are based on weight, and as an example, units of % or ratio mean weight % or weight ratio.

In addition, in the specification of the present invention, the expression “comprises” is an open description that has the same meaning as expressions such as “comprising,” “contains,” “has,” or “characterized by” and is additionally listed. It does not exclude elements, materials or processes that are not present. Also, “substantially… The expression “consists of” means that other elements, materials or processes not listed together with the specified elements, materials or processes do not have an unacceptably significant effect on at least one basic and novel technical idea of the invention. It means something that can exist in quantity. Additionally, the expression “consists of” means that only the elements, materials, or processes described are present.

In the present invention, “sample” or “sample” refers to an object for analysis and is used with the same meaning throughout the specification.

Hereinafter, the Talaromyces strains isolated and identified in the present invention and their uses will be described in detail.

The present invention relates to Talaromyces gwangjuensis CNUFC WT19-1 (Accession number: KCTC14671BP); Talaromyces koreana CNUFC YJW2-13 (Accession number: KCTC14672BP); Alternatively, Talaromyces teleomorha CNUFC YJW2-5 (accession number: KCTC14670BP) is provided.

Talaromyces gwangjuensis CNUFC WT19-1 (accession number: KCTC14671BP) of the present invention; Talaromyces koreana CNUFC YJW2-13 (Accession number: KCTC14672BP); and Talaromyces teleomorha CNUFC YJW2-5 (Accession number: KCTC14670BP) can be manufactured in a form used in various fields such as pharmaceutical compositions, health functional foods, cosmetics, feed, and antibiotics.

The present invention relates to Talaromyces gwangjuensis CNUFC WT19-1 (Accession number: KCTC14671BP); Talaromyces koreana CNUFC YJW2-13 (Accession number: KCTC14672BP); And any one or more strains selected from the group consisting of Talaromyces teleomorha CNUFC YJW2-5 (Accession number: KCTC14670BP); Culture medium of the above strain; and a culture filtrate of the above strain; and provides a pharmaceutical composition comprising as an active ingredient at least one selected from the group consisting of.

In addition to containing the active ingredients, the pharmaceutical composition may further include appropriate carriers, excipients, and diluents commonly used in pharmaceutical compositions.

Carriers, excipients and diluents that may be included in the pharmaceutical composition include, but are not limited to, lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, These include cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxy benzoate, propylhydroxy benzoate, talc, magnesium stearate and mineral oil. When formulating the composition, it can be prepared using diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants.

The pharmaceutical composition can be formulated and used in various forms according to conventional methods. Suitable dosage forms include oral dosage forms such as tablets, pills, powders, granules, dragees, hard or soft capsules, solutions, suspensions or emulsions, injections, and aerosols. Among these, granules and powder pellets are preferred. This is most desirable. Other dosage forms include external preparations, suppositories, and sterile injection solutions, but are not limited thereto.

The pharmaceutical composition can be prepared in a suitable formulation using a pharmaceutically inert organic or inorganic carrier. That is, if the dosage form is a tablet, coated tablet, dragee, or hard capsule, it may contain lactose, sucrose, starch or a derivative thereof, talc, calcium carbonate, gelatin, stearic acid, or a salt thereof. Additionally, when the dosage form is a soft capsule, it may contain vegetable oil, wax, fat, semi-solid and liquid polyol. Additionally, when the formulation is in the form of a solution or syrup, it may include water, polyol, glycerol, and vegetable oil.

In addition to the carrier, the pharmaceutical composition may further include preservatives, stabilizers, wetting agents, emulsifiers, solubilizers, sweeteners, colorants, osmotic pressure regulators, antioxidants, etc.

The present invention relates to Talaromyces gwangjuensis CNUFC WT19-1 (Accession number: KCTC14671BP); Talaromyces koreana CNUFC YJW2-13 (Accession number: KCTC14672BP); And any one or more strains selected from the group consisting of Talaromyces teleomorha CNUFC YJW2-5 (Accession number: KCTC14670BP); Culture medium of the above strain; It provides a health functional food containing as an active ingredient at least one selected from the group consisting of culture filtrate of the above strain.

The term “food” or “food composition” in the present invention refers to meat, sausages, bread, chocolate, candies, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, It includes drinks, alcoholic beverages, vitamin complexes, health functional foods, and health foods, and includes all foods in the conventional sense.

The above-mentioned health functional food (health functional food) is the same term as food for special health use (FoSHU), and is a medical and medical effect processed to efficiently exhibit bioregulatory functions in addition to nutritional supply. It means high food. Here, “function” means adjusting nutrients to the structure and function of the human body or obtaining useful effects for health purposes, such as physiological effects. The food of the present invention can be manufactured by methods commonly used in the industry, and can be manufactured by adding raw materials and ingredients commonly added in the industry. In addition, the formulation of the food can be manufactured without limitation as long as it is a formulation recognized as a food, and the health functional food according to the present invention may be in the form of powder, granules, tablets, capsules, or beverages.

The above-mentioned health food refers to food that has a more active health maintenance or promotion effect compared to general food, and health supplement food refers to food for the purpose of health supplementation. In some cases, the terms health functional food, health food, and health supplement are used interchangeably.

The food composition may further include a physiologically acceptable carrier. The type of carrier is not particularly limited and any carrier commonly used in the art can be used.

Additionally, the food composition may contain additional ingredients that are commonly used in food compositions to improve smell, taste, vision, etc. For example, it may include vitamins A, C, D, E, B1, B2, B6, B12, niacin, biotin, folate, panthotenic acid, etc. In addition, minerals such as zinc (Zn), iron (Fe), calcium (Ca), chromium (Cr), magnesium (Mg), manganese (Mn), copper (Cu), chromium (Cr), and rubidium (Rb). It can be included. Additionally, it may contain amino acids such as lysine, tryptophan, cysteine, and valine.

In addition, the food composition contains preservatives (potassium sorbate, sodium benzoate, salicylic acid, sodium dehydroacetate, etc.), disinfectants (bleaching powder, high bleaching powder, sodium hypochlorite, etc.), antioxidants (butylhydroxyanisole (BHA), butylhydroxide) roxitoluene (BHT), etc.), colorants (tar color, etc.), coloring agents (sodium nitrite, sodium nitrite, etc.), bleach (sodium sulfite), seasoning (MSG, etc.), sweeteners (dulcine, cyclemate, saccharin, sodium) food additives (e.g.), flavorings (vanillin, lactones, etc.), leavening agents (alum, D-potassium hydrogen tartrate, etc.), strengthening agents, emulsifiers, thickeners (grease), coating agents, gum base agents, foam suppressants, solvents, improvers, etc. may contain food additives). The additives can be selected depending on the type of food and used in an appropriate amount.

The food composition of the present invention may further include a foodologically acceptable food auxiliary additive, may be used together with other foods or food ingredients, and may be used appropriately according to conventional methods. The mixing amount of the active ingredient can be appropriately determined depending on the purpose of use (prevention, health, or therapeutic treatment).

The present invention relates to Talaromyces gwangjuensis CNUFC WT19-1 (Accession number: KCTC14671BP); Talaromyces koreana CNUFC YJW2-13 (Accession number: KCTC14672BP); And any one or more strains selected from the group consisting of Talaromyces teleomorha CNUFC YJW2-5 (Accession number: KCTC14670BP); Culture medium of the above strain; It provides a cosmetic composition comprising as an active ingredient at least one selected from the group consisting of culture filtrate of the above-described strain.

Cosmetic compositions according to preferred embodiments of the present invention include solutions, external ointments, creams, foams, nutritional lotions, softening lotions, packs, softening waters, emulsions, makeup bases, essences, soaps, liquid cleansers, bath salts, sunscreen creams, and sun oils. , suspensions, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansers, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays, It is not limited to this.

In addition, the cosmetic composition according to the present invention may further include one or more types of cosmetically acceptable carriers mixed with general skin cosmetics in addition to the above-mentioned functional substances, and common ingredients include, for example, oil, water, Surfactants, humectants, lower alcohols, thickeners, chelating agents, colorants, preservatives, fragrances, etc. can be appropriately mixed, but are not limited to this.

At this time, the cosmetically acceptable carrier included in the cosmetic composition of the present invention may be selected in various ways depending on the formulation of the cosmetic composition. For example, when the formulation of the present invention is an ointment, paste, cream, or gel, the carrier component Animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. can be used, but are not limited to these, and they can be used alone or 2 More than one species can be mixed and used.

In addition, when the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent, or emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, and benzyl benzoate. ate, propylene glycol, 1,3-butyl glycol oil, etc. can be used, especially cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan. Fatty acid esters may be used, but are not limited thereto, and they may be used alone or in a mixture of two or more types.

In addition, when the formulation of the present invention is a suspension, the carrier component includes water, a liquid diluent such as ethanol or propylene glycol, and a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester. , microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant, etc. may be used, but are not limited thereto, and they may be used alone or in a mixture of two or more types.

In addition, when the formulation of the present invention is soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolysates, isethionates, lanolin derivatives, fatty alcohols, vegetable oils, glycerol, sugars, etc. are used as carrier components. may be used, but are not limited to this, and may be used alone or in combination of two or more types.

The present invention relates to Talaromyces gwangjuensis CNUFC WT19-1 (Accession number: KCTC14671BP); Talaromyces koreana CNUFC YJW2-13 (Accession number: KCTC14672BP); And any one or more strains selected from the group consisting of Talaromyces teleomorha CNUFC YJW2-5 (Accession number: KCTC14670BP); Culture medium of the above strain; It provides an antibacterial or antifungal microbial preparation containing as an active ingredient at least one selected from the group consisting of; and a culture filtrate of the above strain.

The antifungal target of the microbial preparation is not particularly limited, and the antifungal target of the microbial preparation is Alternaria alternata , Alternaria mali , Aspergillus fumigatus , Aspergillus oryzae , Basidiobolus ranarum , Botrytis cinerea, Colletotrichum coccodes , Colletotrichum groeosporioi Des ( Colletotrichum gloeosporioides ), Colletotrichum orbiculare , Cylindrocarpon destructans ( Cylindrocarpon destructans ), Fusarium oxysporum f. sp. Lycopersici ( Fusarium oxysporum f. sp. lycopersici ), Fusarium proliferatum ( Lichtheimia corymbifera ), Magnaporthe oryzae ( Magnaporthe oryzae ), Mucor zanseni ( Mucor janssenii ), Pyricularia oryzae , Rhizomucor pusillus , Rhizoctonia solani, Rhizoctonia solani var. It may be selected from the group consisting of Rhizopus stolonifer var. stolonifer , and of course, all targets that exhibit antifungal effects through the microbial agent of the present invention may be included.

The present invention relates to Talaromyces gwangjuensis CNUFC WT19-1 (Accession Number: KCTC14671BP), which has excellent metabolite production activity; Talaromyces koreana CNUFC YJW2-13 (Accession number: KCTC14672BP); and Talaromyces teleomorha CNUFC YJW2-5 (accession number: KCTC14670BP).

The type of the metabolic product is not particularly limited, but depending on the action of the biological metabolic pathway of the strain according to the present invention, for example, apiculide, pentacecilide, tyrandolide, and penicil. Lead, perpactin, vermixocin, etc., as well as mitorubin, botriyodiplodine, rugulovacin and luteoskirin, rubratoxin, austin, austinol, mitorubrin, mitorubry Nol, mitorubrinol acetate, mitorubrinic acid, furfactin; and primary or secondary metabolites such as alternariol, bacillisporin, helicusin, rugulo-vasine A, and talarodercin. More specific examples include cycloleucomerone ( It was confirmed to have excellent production activity for cycloleucomelone, gregatin A, purpactin A, and helicusin.

Talaromyces koreana effectively produced cycloleucomelone, gregatin A, and purpactin A, and Talaromyces teleomorha produced It was confirmed that helicusin was produced effectively. Through this, it was confirmed that the new species isolated and identified through the present invention has the ability to produce a wide range of metabolites. Through this, the strain according to the present invention can be used in the form of a biofactory for the production of useful metabolites. , it is expected to be useful in the food industry, biotechnology industry, etc.

Hereinafter, the contents of the present invention will be described in more detail through examples. The examples are only for illustrating the present invention in more detail, and the scope of the present invention is not limited thereto.

[Example 1]

Identification of fungi

Example 1-1. Sampling and isolation for identification of new fungi

Freshwater samples were collected from Wonhyo Valley in Mudeungsan Mountain, Gwangju, and Jukrim Reservoir in Yeosu in January and May 2017. Samples Samples were transported in sterilized 50 mL conical tubes and stored at 4°C.

Before culturing, all samples were diluted with sterilized distilled water to reduce density and improve strain recovery. Specifically, each sample was shaken at room temperature for 15 minutes, and a 100 uL aliquot of each sample was mixed with 9 mL of sterile distilled water.

Next, the mixture was successively diluted 10-fold from 10 ^-1 to 10 ^-4 to prepare step-by-step dilutions. The antibiotic streptomycin (final concentration 50 ppm; Sigma-Aldrich, St. Louis, MO, USA) was added, and 39 g potato dextrose agar (PDA; Becton, Dickinson, and Co., Sparks) in 1 L deionized water. MD, USA), a 100 uL aliquot of each dilution was applied to a Petri dish plate containing medium.

Each plate was cultured at 25°C for 5 to 10 days.

Afterwards, individual colonies of various shapes were selected, transferred to a plate, and subcultured until a pure culture was obtained, thereby obtaining pure isolates.

The ex-type biological culture was stored in the fungal herbarium (Fungarium) of the Chonnam National University Environmental Microbiology Laboratory (CNUFC), and the dried culture was stored in the herbarium (Herbarium) of Chonnam National University.

Example 1-2. Morphological analysis

For detailed morphological studies, the isolated strains were cultured on Czapek yeast autolysate agar (CYA) medium, malt extract agar (MEA) medium, and yeast extract sucrose agar (YES) medium. , oatmeal agar (OA) medium, dichloran 18% glycerol (DG18) agar medium, CYA supplemented with 5% NaCl (CYAS), creatine sucrose agar (CREA) Each of these smeared Petri dishes was inoculated in a 3-point format.

Each plate was incubated at 5, 10, 20, 25, 30, and 37°C for 7 days.

The medium preparation, inoculation, and culture were performed according to the method disclosed in Yilmaz et al.

The characteristics of each colony were recorded after 7 days.

After culturing, the strain was mounted in lactic acid solution (Junsei Chemical Co. Ltd., Tokyo, Japan), and excess conidia were removed with 96% ethanol.

Then, digital images were obtained using an Olympus BX51 microscope equipped with DIC optics (Olympus, Tokyo, Japan).

The microhomogeneous structure was observed by scanning electron microscopy (SEM) (Hitachi S4700; Hitachi, Tokyo, Japan). The process for the SEM analysis was performed according to the method disclosed in Nguyen et al.

Example 1-3. Morphological analysis results

Talaromyces gwangjuensis (Accession number: KCTC14671BP) and Talaromyces koreana (Accession number: KCTC14672BP) newly isolated and identified in the present invention; and Talaromyces teleomorha (Accession number: KCTC14670BP) and adjacent strains are summarized in Tables 1 to 3 below.

1) Talaromyces gwangjuensis : Fungus index IF554801

[Table 1]

Talaromyces Gwangjuensis (ex-type organism standard, ex-type) was confirmed to contain austin, austinol, other austins, mitorubrin, and mitoru as a result of extracellular culture confirmation. It produced mitorubrinol, mitorubrinol acetate, mitorubrinic acid, and purpactin.

Talalomyces radimirici formed the same group as Talalomyces radimirici, but Talalomyces radimirici formed smaller colonies in CYA and YEA media at 25°C. They differ morphologically from each other in that there is a greater number of pialids per Metulae and Merulae compared to Myces radimyrichs.

In addition, Talaromyces Gwangjuensis produced spherical conidia (globose conidia), unlike the ellipsoid conidia of Talaromyces radimyrich, and Talaromyces radimyrich grew at 37°C. Although it was possible, Talaromyces Gwangjuensis showed such a difference that growth was impossible.

2) Talaromyces koreana : Fungus index IF554802

[Table 2]

As a result of extracellular culture, Talaromyces Koreana (ex-type) produced cycloleucomelone, gregatin A, and purpactin A.

Talaromyces coreana is phylogenetically closely related to Talaromyces boninensis and Talaromyces reverso-olivaceus .

Morphologically, Talaromyces Koreana has more pialids per Metulae compared to Talaromyces bonenensis and Talaromyces Reverso-olivaceus, and Metulae has greater characteristics than the above strains. There is.

Additionally, Talaromyces Koreana produced smaller conidia than the above strain. Talaromyces bonenensis and Talaromyces reverso-olibaceus have maximum reported colony diameters of 30 and 34-37 mm when cultured in MEA at 25°C for 7 days, while Talaromyces coreana There was a large difference between 41 and 45 mm.

3) Talaromyces teleomorha : fungus index IF554803

[Table 3]

As a result of extracellular culture, Talaromyces teleomorpha produced helicusin, which was previously discovered in Talaromyces helicus .

Talaromyces teleomorpha grew rapidly to 26-29 mm, 29-33 mm, and 45-48 mm in CYA, YES, and MEA media at 25°C for 7 days, respectively, and through this, the related species Talaromyces helicus It can be easily distinguished from.

In particular, in MEA at 25°C, the asci of Talaromyces helicus are smaller than those of Talaromyces teleomorpha, and Talaromyces teleomorpha resembles the asexual form present in Talaromyces helicus. did not create

Example 1-4. DNA extraction, PCR and sequencing

The isolated and stored strains were cultured on PDA covered with cellophane for 5-7 days at 25°C.

Afterwards, genomic DNA was extracted from the fungal mycelium using the Solg ^TM Genome DNA prep kit (Solgent, Daejeon, Korea).

Protocols for genes and amplification regions were used according to the information disclosed in the following literature.

The internal transcribed spacer (ITS) region of rDNA was amplified using the primer pair ITS1 and ITS4 (White TJ, Bruns T, Lee S, et al. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In : PCR protocols: a guideto methods and applications (Innis MA, Gelfand DH, Shinsky TJ, White TJ, eds). Academic Press Inc, New York: 315-32).

β-tubulin (BenA) was amplified using the primer pair T10 and Bt2b (Glass, NL; Donaldson, GC Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl. Environ. Microbiol . 1995 , 6 , 1323-1330).

Calmodulin (CaM) gene was amplified using primer pairs CMD5/CMD6 and CF1/CF4 (Peterson, SW; Vega, F.; Posada, F.; Nagai, C. Penicillium coffeae , a new endophytic species isolated from a coffee plant and its phylogenetic relationship to P. fellutanum , P. thiersii and P. brocae based on parsimony analysis of multilocus DNA sequences. Mycologia . 2005 , 97 , 659-666., Hong, SB; Cho, HS; Shin, HD; Frisvad , JC; Samson, RA Novel Neosartorya species isolated from soil in Korea. Int. J. Syst. Evol . Microbiol . 2006 , 56 , 477-486.).

The primer pair RPB2-5F and RPB2-7cR were used to amplify the RPB2 gene region (Liu, YJ; Whelen, S.; Hall, BD Phylogenetic relationships among ascomycetes: evidence from an RNA polymerase II subunit. Mol. Biol. Evol . 1999 , 16 , 1799-1808.).

PCR amplification was performed as described by Houbraken and Samson and Yilmaz et al. It was performed according to the information disclosed in the literature (Houbraken J.; Samson RA Phylogeny of Penicillium and the segregation of Trichocomaceae into three families. Stud. Mycol . 2011 , 70 , 1-51., Yilmaz, N.; Visagie, CM; Houbraken , J.; Frisvad, JC; Samson, R.A. Polyphasic taxonomy of the genus Talaromyces . Stud. Mycol . 2014 , 78 , 175-341).

The PCR amplification mixture (total volume, 20 μl) contained a fungal DNA template, 5 pmol/μl each primer, and Accupower PCR Premix (Bioneer, Daejeon, Korea). PCR products were purified using Accuprep PCR PurificationKit (Bioneer Corp., Daejeon, Korea) according to the manufacturer's instructions. DNA sequencing was performed using an ABI 3700 automated DNA sequencer (Applied Biosystems Inc., Foster City, CA, USA).

Examples 1-5. Phylogenetic analysis

Each generated sequence was checked for the presence of ambiguous bases and assembled using the Lasergene SeqMan (DNASTAR, Inc., Madison, WI, USA) program.

The edited sequences were subjected to a BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi) search against the nucleic acid database of NCBI GenBank to search for the closest relatives.

Sequences of all registered Talaromyces species were downloaded from Genbank and used. Each sequence was aligned using MAFFT (http://mafft.cbrc.jp/alignment/server), and the alignment result was trimmed using trimAI and then combined with MEGA 7.

Each data in FASTA format was converted to nexus and phylip format using the online program Align-ment Transformation Environment (https://sing.ei.uvigo.es/ALTER/).

Phylogenetic reconstruction using maximum likelihood analysis (ML) was performed using RAxML-HPC2 in XSEDE on the CIPRES portal (https://www.phylo.org/portal2) with 1000 bootstrap replicates, and GTR + The GAMMA nucleotide evolution model was used.

Bayesian Inference Analysis was performed using MrBayes 3.2.2 using the Markov Chain Monte Carlo (MCMC) algorithm. The sample frequency was set to 100, and the first 25% of the tree was removed as burn-in.

Phylogenetic analysis results were obtained from FigTree ver. Visualization was performed using 1.3.1. At this time, the auxiliary values are ML bootstrap support (BS) and BI posterior probability (PP), which are provided to the branches of the tree.

At this time, Talaromyces tenuis CBS 141840 was selected as an outgroup in the Helici and Purpurei sections, and Trichocoma paradoxa CBS 788.83 was selected as an outgroup in Talaromyces. It was selected as an outgroup in the integrated phylogenetic tree of the species.

The sequences used for phylogenetic analysis are listed in Table 1 below.

Among these, the newly obtained sequence through the present invention was deposited in the NCBI GenBank database and was assigned the accession numbers shown in Table 4 below.

GenBank accession numbers of fungi used for phylogenetic analysis Taxon name Strain no. GenBank accession no. ITS BenA CaM RPB2 T. aerugineus CBS 350.66 ^T AY753346 KJ865736 KJ885285 JN121502 T. apiculatus CBS 312.59 ^T JN899375 KF741916 KF741950 KM023287 T. atricola CBS 255.31 ^T KF984859 KF984566 KF984719 KF984948 T. atroroseus CBS ^133442T KF114747 KF114789 KJ775418 KM023288 T. austrocalifornicus CBS 644.95 ^T JN899357 KJ865732 KJ885261 MN969147 T. bacillisporus CBS 296.48 ^T KM066182 AY753368 KJ885262 JF417425 T. bohemicus CBS 545.86 ^T JN899400 KJ865719 KJ885286 JN121532 T. boninensis CBS ^650.95T JN899356 KJ865721 KJ885263 KM023276 T. borbonicus CBS ^141340T MG827091 MG855687 MG855688 MG855689 T. brunneosporus FMR ^16566T LT962487 LT962483 LT962488 LT962485 T. cecidicola CBS ^101419T AY787844 FJ753295 KJ885287 KM023309 T. cinnabarinus CBS 267.72 ^T JN899376 AY753377 KJ885256 JN121477 T. cinnabarinus CBS 357.72 - KM066134 - - T. chlamydosporus CBS ^140635T KU866648 KU866836 KU866732 KU866992 T. chlorolomus DAOM 241016 ^T FJ160273 GU385736 KJ885265 KM023304 T. chlorolomus DTO 180-F4 - FJ753294 - - T. chlorolomus DTO 182-A5 - JX091597 - - T. cnidii KACC ^46617T KF183639 KF183641 KJ885266 KM023299 T. cinnabarinus CBS 267.72 ^T JN899376 AY753377 KJ885256 JN121477 T. cinnabarinus CBS 357.72 - KM066134 - - T. coalescens CBS ^103.83T JN899366 JX091390 KJ885267 KM023277 T. columbinus NRRL ^58811T KJ865739 KF196843 KJ885288 KM023270 T. dendriticus CBS 660.80 ^T JN899339 JX091391 KF741965 KM023286 T. dendriticus DAOM 226674 - FJ753293 - - T. dendriticus DAOM 233861 - FJ753294 - - T. derxii CBS 412.89 ^T JN899327 JX494306 KF741959 KM023282 T. diversiformis CBS ^141931T KX961215 KX961216 KX961259 KX961274 T. diversus CBS 320.48 ^T KJ865740 KJ865723 KJ885268 KM023285 T. duclauxii CBS 322.48 ^T JN899342 JX091384 KF741955 JN121491 T. emodensis CBS ^100536T JN899337 KJ865724 KJ885269 JF417445 T. erythromellis CBS 644.80 ^T JN899383 HQ156945 KJ885270 KM023290 T. euchlorocarpius DTO 176-I3 ^T AB176617 KJ865733 KJ885271 KM023303 T. flavus CBS 310.38 ^T JN899360 JX494302 KF741949 JF417426 T. fusiformis CBS ^140637T KU866656 KU866843 KU866740 KU867000 T. georgeiensis ^DI16-145T LT558967 LT559084 - LT795606 T. gwangjuensis CNUFC WT19-1 ^T MK766233 MZ318448 - MK912174 T. gwangjuensis CNUFC WT19-2 MK766234 MZ318449 - MK912175 T. helicus CBS 335.48 ^T JN899359 KJ865725 KJ885289 KM023273 T. helicus CBS 134.67 - KM066133 - - T. iowaense NRRL ^66822T MH281565 MH282578 MH282579 MH282577 T. islandicus CBS 338.48 ^T KF984885 KF984655 KF984780 KF985018 T. korena CNUFC YJW2-13 ^T MZ315100 MZ318450 MZ332529 MZ332533 T. korena CNUFC YJW2-14 MZ315101 MZ318451 MZ332530 MZ332534 T. mimosinus CBS 659.80 ^T JN899338 KJ865726 KJ885272 MN969149 T. minioluteus CBS 642.68 ^T JN899346 MN969409 KJ885273 JF417443 T. palmae CBS 442.88 ^T JN899396 HQ156947 KJ885291 KM023300 T. piceus CBS 361.48 ^T KF984792 KF984668 KF984680 KF984899 T. pigmentosus CBS ^142805T MF278330 LT855562 LT855565 LT855568 T. pittii CBS 139.84 ^T JN899325 KJ865728 KJ885275 KM023297 T. proteolyticus CBS 303.67 ^T JN899387 KJ865729 KJ885276 KM023301 T. pseudostromaticus CBS 470.70 ^T JN899371 HQ156950 KJ885277 KM023298 T. ptychoconidius DAOM 241017 ^T FJ160266 GU385733 JX140701 KM023278 T. ptychoconidius DTO 180-E9 - GU385734 - - T. ptychoconidius DTO 180-F1 - GU385735 - - T. purpureogenus CBS 286.36 ^T JN899372 JX315639 KF741947 JX315709 T. purpureus CBS 475.71 ^T JN899328 GU385739 KJ885292 JN121522 T. rademirici CBS 140.84 ^T JN899386 KJ865734 KM023302 T. radicus CBS 100489 ^T KF984878 KF984599 KF984773 KF985013 T. ramulosus DAOM ^241660T EU795706 FJ753290 JX140711 KM023281 T. ramulosus DTO 182-A6 - JX091631 - - T. ramulosus DTO 181-E3 - JX091626 - - T. ramulosus DTO 182-A3 - JX091630 - - T. reverso-olivaceus CBS ^140672T KU866646 KU866834 KU866730 KU866990 T. rotundus CBS 369.48 ^T JN899353 KJ865730 KJ885278 KM023275 T. rugulosus CBS 371.48 ^T KF984834 KF984575 KF984702 KF984925 T. ryukyuensis NHL 2917 ^T AB176628 - - - T. stipitatus CBS 375.48 ^T JN899348 KM111288 KF741957 KM023280 T. subinflatus CBS 652.95 ^T JN899397 MK450890 KJ885280 KM023308 T. tabacinus NRRL ^66727T MG182613 MG182627 MG182606 MG182620 T. tardifaciens CBS 250.94 ^T JN899361 KF984560 KF984682 KF984908 T. teleomorpha CNUFC YJW2-5 ^T MZ315102 MZ318452 MZ332531 MZ332535 T. teleomorpha CNUFC YJW2-6 MZ315103 MZ318453 MZ332532 MZ332536 T. tenuis CBS 141840 ^T MN864275 MN863344 MN863321 MN863333 T. trachyspermus CBS 373.48 ^T JN899354 KF114803 KJ885281 JF417432 T. tratensis CBS ^133146T KF984891 KF984559 KF984690 KF984911 T. ucrainicus CBS 162.67 ^T JN899394 KF114771 KJ885282 KM023289 T. unicus CBS ^100535T JN899336 KJ865735 KJ885283 MN969150 T. varians CBS 386.48 ^T JN899368 KJ865731 KJ885284 KM023274 T. verruculosus NRRL ^1050T KF741994 KF741928 KF741944 KM023306 T. viridulus CBS 252.87 ^T JN899314 JX091385 KF741943 JF417422 Trichocoma paradoxa CBS 788.83 ^T JN899398 KF984556 KF984670 JN121550

In the table above, bold letters indicate the strain and accession number of the present invention, and information on the abbreviations used is as follows.

CBS: Culture collection of the Westerdijk Fungal Biodiversity Institute, The Netherlands.

CNUFC: Chonnam National University Fungal Collection, Gwangju, South Korea;

DAOM: Agriculture Canada and Agri-Food Canada Culture Collection, Ottawa, ON, Canada;

DTO: Internal Culture Collection of the CBS-Fungal Biodiversity Center;

FMR: Facultat de Medicina i Ciencies de la Salut, Reus, Spain;

KACC: Korean Agricultural Culture Collection, Republic of Korea;

NRRL: Agricultural Research Service Culture Collection, Peoria, Illinois, U.S.;

^T : ex-type strain.

Example 1-6. Phylogenetic analysis

Phylogenetic relationships within Talaromyces were derived using concatenated data sets of four loci: ITS, BenA, CaM, and RPB2. The results are shown in Figure 1.

In the above results, 67 taxa were included in the multi-gene analysis, including Trichocoma paradoxa CBS 788.83 as an outgroup.

The concatenated alignment consisted of 2407 characters containing 425, 443, 687, and 852 gaps used in ITS, BenA, CaM, and RPB2, respectively.

According to Figure 1, there are eight major line-ages in Talaromyces , which are as described by Yilmaz et al. and Sun et al. (Sun, BD; Chen, AJ; Houbraken, J.; Frisvad, JC; Wu, WP; Wei, HL; Zhou, YG; Jiang, XZ; Samson, R.A. New section and species in Talaromyces . Mycokeys . 2020 , 68 , 75-113.) is consistent with the sectional classification.

Talaromyces gwangjuensis CNUFC WT19-1 (Accession Number: KCTC14671BP) according to the present invention; Talaromyces koreana CNUFC YJW2-13 (Accession number: KCTC14672BP); and Talaromyces teleomorha CNUFC YJW2-5 (accession number: KCTC14670BP) was confirmed to belong to the Purpurei and Helici families by multiple gene analysis, and is a small genus including T. brunne-osporus highlighted by an asterisk. The clade is not assigned to any known lineage.

In the Purpurei lineage, Talaromyces gwangjuensis was clustered close to, but separate from, Talaromyces rademirici in the monophyletic lineage (BenA, RPB2, ITS) and in the combined lineage ( Figure 2).

In the Helici family, Talaromyces teleomorha is close to Talaromyces helicus, in monophyletic (BenA, RPB2, ITS) and combined phylogenies, but CaM and RPB2 Talaromyces helicus , Talaromyces koreana , Talaromyces reverse-olivaceus and Talaromyces boninensis in the phylogenetic tree. ) was located between (Figure 3).

Additionally, Talaromyces koreana is related to Talaromyces reverse- olivaceus and Talaromyces boninensis in the BenA, CaM, RPB2 and combined lineages. It was confirmed that there was (Figure 3). However, in the ITS phylogenetic analysis , Talaromyces koreana was confirmed to be adjacent only to Talaromyces boninensis .

Example 1-7. Extrolite analysis

Extracellular cultures (extrolites) were extracted from Talaromyces strains cultured for 7 to 10 days in CYA, YES, and MEA media at 25°C.

Preparation and analysis of extracts were performed as described previously by Frisvad and Thrand, Nielsen et al. and Houbraken et al. (Frisvad, JC; Thrane, U. Standardized high performance liquid chromatography of 182 mycotoxins and other fungal metabolites based on alkylphenone indices and UV VIS spectra (diode array detection). J. Chromatogr . 1987 , 404 , 195214., Nielsen, K.F.; Mansson, M.; Rank, C.; Frisvad, J.C.; Larsen, TO. Dereplication of microbial natural products by LC-DAD-TOFMS. J. Nat. Prod . 2011 , 74 , 2338 -2348., Houbraken, J.; Wang, L.; Lee, HB; Frisvad, JC New sections in Penicillium containing novel species producing patulin, pyripyropens or other bioactive compounds. Persoonia . 2016 , 36 , 299-314.).

Examples 1-8. Extrolite analysis results

It was confirmed that the new species isolated and identified through the present invention has the ability to produce a wide range of metabolites, and is expected to be useful in the food industry and biotechnology industry.

In particular, through analysis of the extrolite profile, apiculide, pentacecilide, tyrandolide, penicillide, perpactin, vermixocin, etc., as well as mitorubin, botriyodiplodin, rugulovacin, and lutein It was confirmed that the production of theoskirin, rubratoxin, austin, austinol, mitorubrin, mitorubrinol, mitorubrinol acetate, mitorubrinic acid, furfactin, etc. was possible.

In addition, some secondary metabolites such as alternariol, bacillisporin, and helicusin were found in the Helici section, and it was confirmed that rugulo-vasine A, talarodercin, etc. could be produced.

[Example 2]

New Talaromyces Gwangjuensis ( Talaromyces gwangjuensis ) CNUFC WT19-1, Talaromyces Koreana ( Talaromyces koreana ) CNUFC YJW2-13 and Talaromyces teleomorpha ( Talaromyces teleomorha ) Measurement of CNUFC YJW2-5 antifungal activity

Talaromyces gwangjuensis CNUFC WT19-1 (Accession number: KCTC14671BP), Talaromyces koreana CNUFC YJW2-13 (Accession number: KCTC14672BP), and Talaromyces teleomorpha ( Talaromyces teleomorha ) The antifungal activity of CNUFC YJW2-5 (accession number: KCTC14670BP) was measured using a dual culture method in PDA medium.

Fungal pathogens Alternaria alternata , Botrytis cinerea, Colletotrichum gloeosporioides , Fusarium proliferatum , and Piricula Pyricularia oryzae, Mucor janssenii , Lichtheimia corymbifera , Rhizomucor pusillus , Basidiobolus ranarum , and Mycelial plugs of Aspergillus fumigatus ( Aspergillus fumigatus ) cultured for 7 days were placed in the center of each PDA plate, and Talaromyces gwangjuensis ( Talaromyces gwangjuensis ) CNUFC WT19-1, Tala, cultured for 7 days Talaromyces koreana CNUFC YJW2-13 and Talaromyces teleomorha CNUFC YJW2-5 strain cultures were placed 2.5 cm away from the fungal pathogen, respectively.

All plates were incubated in the dark at 25°C for 5-7 days, and each experiment was repeated three times. Control plates contained only hyphal plugs of each fungal pathogen.

Afterwards, antagonistic activity was confirmed by measuring mycelial growth with the control group.

As a result, Talaromyces gwangjuensis CNUFC WT19-1 strain is Alternaria alternata , Botrytis cinerea , and Colletotrichum groeosporioides. gloeosporioides ), Fusarium proliferatum , Pyricularia oryzae, Mucor janssenii , Lichtheimia corymbifera , Rhizomeucor fucilus ( It was confirmed that it inhibits the mycelium growth of 10 pathogens, including Rhizomucor pusillus, Basidiobolus ranarum , and Aspergillus fumigatus (Figure 1).

In addition, Talaromyces teleomorha CNUFC YJW2-5 is Alternaria alternata , Pyricularia oryzae , Fusarium proliferatum , and It inhibited the mycelial growth of four pathogens, including Rhizomucor pusillus (Figure 2A-Figure 2D), and Talaromyces koreana CNUFC YJW2-13 inhibited Pyricularia . oryzae ) was confirmed to inhibit mycelial growth of the pathogen (Figure 2E).

As shown above, through the present invention, three new species were obtained from fungi living in domestic freshwater, Talaromyces gwangjuensis CNUFC WT19-1 (accession number: KCTC14671BP), and Talaromyces Koreana. koreana ) CNUFC YJW2-13 (accession number: KCTC14672BP) and Talaromyces teleomorha ( Talaromyces teleomorha ) CNUFC YJW2-5 (accession number: KCTC14670BP) were isolated and identified, and through phylogenetic analysis with related species, Phylogenetic correlation was identified, and through antifungal activity analysis, it was confirmed that it exhibits antifungal activity against 1 to 10 types of pathogens depending on the strain.

As above, specific parts of the content of the present invention have been described in detail, and for those skilled in the art, it is clear that these specific techniques are merely preferred embodiments and do not limit the scope of the present invention. It will be obvious. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Korea Research Institute of Bioscience and Biotechnology KCTC14671BP 20210824 Korea Research Institute of Bioscience and Biotechnology KCTC14672BP 20210824 Korea Research Institute of Bioscience and Biotechnology KCTC14670BP 20210824

Claims (6)

Talaromyces gwangjuensis CNUFC WT19-1 (Accession number: KCTC14671BP). delete Talaromyces gwangjuensis CNUFC WT19-1 (accession number: KCTC14671BP) strain;
Culture medium of the above strain; and
Culture filtrate of the above strain;
A health functional food containing as an active ingredient one or more selected from the group consisting of. Talaromyces gwangjuensis CNUFC WT19-1 (accession number: KCTC14671BP) strain;
Culture medium of the above strain; and
Culture filtrate of the above strain;
A cosmetic composition containing as an active ingredient one or more selected from the group consisting of. Talaromyces gwangjuensis CNUFC WT19-1 (accession number: KCTC14671BP) strain;
Culture medium of the above strain; and
Culture filtrate of the above strain;
An antifungal microbial preparation containing as an active ingredient at least one selected from the group consisting of. According to clause 5,
Antifungal targets of the microbial preparation include Alternaria alternata , Aspergillus fumigatus , Basidiobolus ranarum , Botrytis cinerea , and Cholesterol. Colletotrichum gloeosporioides , Fusarium proliferatum , Lichtheimia corymbifera , Mucor janssenii , Piricularia duckweed ( An antifungal microbial agent selected from the group consisting of Pyricularia oryzae ) and Rhizomucor pusillus .