KR102620922B1 - Bio support of three dimensional structure using electrospinning apparatus - Google Patents

Abstract

The present invention relates to a three-dimensional bio scaffold using an electrospinning device that can simultaneously provide anti-adhesion and wound coverage by forming an anti-adhesion layer and a wound coating layer using an electrospinning device. To this end, the present invention may include a first nanofiber layer manufactured by electrospinning and having a first porosity, and a second nanofiber layer electrospun on the first nanofiber layer and having a second porosity smaller than the first porosity.

Description

Bio support with 3D structure using electrospinning device {BIO SUPPORT OF THREE DIMENSIONAL STRUCTURE USING ELECTROSPINNING APPARATUS}

The present invention relates to a three-dimensional bio support using an electrospinning device, and more specifically, to an electrospinning device that can simultaneously provide anti-adhesion and wound coverage by forming an anti-adhesion layer and a wound coating layer using an electrospinning device. This relates to a bio-support with a three-dimensional structure using .

Technologies for manufacturing nanofibers include composite spinning, melt blow spinning, flash spinning, and electrospinning. Among these nanofiber manufacturing technologies, nanofiber manufacturing technology by electrospinning is recognized as the most promising technology in terms of polymer diversity, simplicity of manufacturing process, commercialization potential, and applicability to various technologies.

Nanofiber manufacturing technology by electrospinning is a spinning method in which a high voltage is applied to a polymer solution, the polymer solution is ejected, the solvent is volatilized, and a fiber with a nanoscale diameter is obtained.

Nanofibers manufactured using electrospinning technology are known to have high specific surface area, porosity, high aspect ratio, and flexibility, and that it is easy to control the fiber diameter by adjusting the spinning conditions of the electrospinning device.

Recently, research on medical materials such as wound dressings, drug delivery systems (DDS), and scaffolds for tissue engineering using electrospun nanofibers has been in the spotlight.

In particular, among the electrospun nanofiber scaffolds for tissue engineering, wound coating agents can have a structure very similar to the extracellular matrix (ECM) in vivo, so they can attach to the scaffold for cells and provide an environment for growth on the scaffold. You can.

The cell growth environment in these nanofiber scaffolds must have high porosity to enable high density cell adhesion, and the porosity and pore shape must be adjustable depending on the characteristics of the tissue being cultured.

In addition, among the electrospun nanofiber scaffolds for tissue engineering, the anti-adhesion film or barrier film can provide an anti-adhesion environment for a long period of time due to the cells not adhering to the scaffold.

In order to provide an efficient cell growth environment for these nanofiber supports or to provide an anti-adhesion environment to prevent long-term adhesion, it must be possible to control the pore size of the nanofibers and the hydrophobic and hydrophilic properties of the nanofibers depending on the characteristics of the tissue.

In order to provide the various functions described above to electrospun nanofiber supports, various studies are being conducted on three-dimensional electrospun nanofiber supports.

However, conventional 3D electrospun nanofiber supports are manufactured for wound coating or for preventing adhesion, so there are limitations in simultaneously performing wound covering and adhesion prevention.

Republic of Korea Patent Publication No. 10-1997966 (2019.07.02, registered)

Therefore, the present invention was derived to solve the above-mentioned problems. The present invention is a three-dimensional electrospinning device that can provide both adhesion prevention and wound coverage by forming an anti-adhesion layer and a wound coating layer using an electrospinning device. The purpose is to provide a bio-support structure.

In addition, the present invention provides a three-dimensional bio scaffold using an electrospinning device in which the porosity of the anti-adhesion layer and the porosity of the wound coating layer are adjusted by electrospinning to suit the environment for cell attachment or non-attachment and the cell proliferation environment. There is.

Another object of the present invention is to provide a three-dimensional bio-support using an electrospinning device in which the hydrophilic and hydrophobic properties of the anti-adhesion layer and the hydrophilic and hydrophobic properties of the wound coating layer are controlled.

In addition, the present invention provides a three-dimensional structure using an electrospinning device in which the porosity of the wound coating layer is adjusted differently in the depth direction or the porosity of the wound coating layer is patterned to promote wound healing by maximizing tissue growth in the area in contact with cells. There is another purpose in providing a bio-support.

In addition, the present invention is an electrospinning method that can differently adjust the porosity of the wound coating layer in contact with the wound area and the wound coating layer in contact with the outer part of the wound area so that cell proliferation is concentrated in the wound area and the wound coating layer does not fall off from the wound area. Another purpose is to provide a bio-support with a three-dimensional structure using a device.

Other objects of the present invention will become clearer through the examples described below.

A three-dimensional bio support using an electrospinning device according to an aspect of the present invention is manufactured by electrospinning and is electrospun on a first nanofiber layer and a first nanofiber layer having a first porosity and a second porosity smaller than the first porosity. It may include a second nanofiber layer with.

In addition, the first nanofiber layer includes an 11th nanofiber layer in contact with the second nanofiber layer, a 13th nanofiber layer located on the outermost side of the first nanofiber layer, and a 12th nanofiber layer located between the 11th nanofiber layer and the 13th nanofiber layer. It includes a fiber layer, and the 11th porosity of the 11th nanofiber layer may be smaller than the 12th porosity of the 12th nanofiber layer, and the 12th porosity of the 12th nanofiber layer may be smaller than the 13th porosity of the 13th nanofiber layer.

Additionally, the 11th porosity may be 83 to 85%, the 12th porosity may be 84 to 86%, and the 13th porosity may be 85 to 87%.

In addition, the first nanofiber layer includes a first nanofiber support portion, a nanofiber pattern portion extending from the first nanofiber support portion, and a second nanofiber support portion extending from the nanofiber pattern portion, and the pattern porosity of the nanofiber pattern portion is The first support porosity of the first nanofiber support may be greater than the second support porosity of the second nanofiber support.

Additionally, the nanofiber pattern portion may be more hydrophilic than the first nanofiber support portion and the second nanofiber support portion.

In addition, it may further include a hydrophobic coating layer coupled to the second nanofiber layer.

A three-dimensional bio-support using an electrospinning device according to an embodiment of the present invention provides the following effects.

The present invention has the effect of providing both anti-adhesion and wound coverage at the same time by forming an anti-adhesion layer and a wound coating layer using an electrospinning device.

The present invention has the effect of adjusting the porosity of the anti-adhesion layer and the porosity of the wound coating layer by electrospinning to suit the environment for cell attachment or non-adhesion and the cell proliferation environment.

The present invention has the effect of controlling the hydrophilic and hydrophobic properties of the anti-adhesion layer and the hydrophilic and hydrophobic properties of the wound coating layer.

The present invention has the effect of controlling the porosity of the wound coating layer differently in the depth direction or patterning the porosity of the wound coating layer to promote wound healing by maximizing tissue growth in the area in contact with cells.

The present invention has the effect of controlling the porosity of the wound coating layer in contact with the wound area and the wound coating layer in contact with the outer part of the wound area differently, so that cell proliferation is concentrated in the wound area and the wound coating layer does not fall off from the wound area.

The effects of the present invention are not limited to those mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the description below.

Figure 1 is a diagram schematically showing a cross section of a three-dimensional bio-support using an electrospinning device according to a first embodiment of the present invention.
Figure 2 is an image for explaining the multilayer structure of a three-dimensional bio support using an electrospinning device according to the first embodiment of the present invention.
Figure 3 is a diagram schematically showing a cross section of a three-dimensional bio-support using an electrospinning device according to a second embodiment of the present invention.
Figure 4 is an image for explaining the nanofiber diameter of the first nanofiber layer of a three-dimensional bio support using an electrospinning device according to the second embodiment of the present invention.
Figure 5 is an image for explaining the nanofiber shape of the second nanofiber layer of the three-dimensional bio support using an electrospinning device according to the second embodiment of the present invention.
Figure 6 is a diagram schematically showing a cross section of a three-dimensional bio-support using an electrospinning device according to a third embodiment of the present invention.
Figure 7 is a diagram schematically showing a cross section of a three-dimensional bio-support using an electrospinning device according to a fourth embodiment of the present invention.
Figure 8 is a diagram schematically showing a cross section of a three-dimensional bio-support using an electrospinning device according to the fifth embodiment of the present invention.

Since the present invention can be modified in various ways and can have various embodiments, specific embodiments will be illustrated in the drawings and described in detail in the detailed description. However, this is not intended to limit the present invention to specific embodiments, and should be understood to include all transformations, equivalents, and substitutes included in the spirit and technical scope of the present invention. In describing the present invention, if it is determined that a detailed description of related known technologies may obscure the gist of the present invention, the detailed description will be omitted.

The terms used in this application are only used to describe specific embodiments and are not intended to limit the invention. Singular expressions include plural expressions unless the context clearly dictates otherwise. In this application, terms such as “comprise” or “have” are intended to designate the presence of features, numbers, steps, operations, components, parts, or combinations thereof described in the specification, but are not intended to indicate the presence of one or more other features. It should be understood that this does not exclude in advance the possibility of the existence or addition of elements, numbers, steps, operations, components, parts, or combinations thereof.

Terms such as first, second, etc. may be used to describe various components, but the components should not be limited by the terms. The above terms are used only for the purpose of distinguishing one component from another.

Hereinafter, embodiments according to the present invention will be described in detail with reference to the accompanying drawings. In the description with reference to the accompanying drawings, identical or corresponding components will be assigned the same reference numbers regardless of the reference numerals, and duplicates thereof will be provided. Any necessary explanation will be omitted.

Hereinafter, a three-dimensional bio-support using an electrospinning device according to embodiments of the present invention will be described in detail with reference to FIGS. 1 to 8.

Figure 1 is a diagram schematically showing a cross section of a three-dimensional bio-support using an electrospinning device according to a first embodiment of the present invention.

As shown in FIG. 1, the three-dimensional bio support 100 using the electrospinning device according to this embodiment may include a first nanofiber layer 110 and a second nanofiber layer 120.

The first nanofiber layer 110 is a wound coating layer and may include PGA (Polyglycolic Acid), which has high hydrophilic properties. The first nanofiber layer 110 contains PGA material, which has greater hydrophilic properties than hydrophobic properties, so it is easily biodegradable and adheres well to cells, which can be advantageous for cell deposition.

The first nanofiber layer 110 is manufactured by electrospinning and may have a first porosity. The diameter of the nanofibers, the density of the nanofibers, and the first porosity of the first nanofiber layer 110 can be adjusted by electrospinning.

The first porosity is a value expressed as a ratio of the pore volume between nanofibers to the volume of the entire nanofiber, and can have a larger value as the pores become larger. The first porosity may be set to 80% or more to favor cell attachment and proliferation.

Here, the porosity of the nanofiber layer can affect the cell proliferation rate in the nanofiber layer. In detail, increasing the porosity of the nanofiber layer can facilitate the deposition of cells in the enlarged pores of the nanofiber layer, thereby promoting cell proliferation in the nanofiber layer. On the other hand, if the porosity of the nanofiber layer is reduced, it becomes difficult to deposit cells in the pores of the nanofiber layer, which may inhibit cell proliferation in the nanofiber layer.

In addition, by decreasing or increasing the hydrophilicity of the nanofiber layer, the time for cells to be deposited in the pores of the nanofiber layer can be adjusted. Therefore, by adjusting the hydrophilic nature of the nanofiber layer along with the porosity of the nanofiber layer, the cell proliferation rate and direction of cell proliferation can be controlled. do.

Therefore, according to this embodiment, by adjusting the first porosity and hydrophilic properties of the first nanofiber layer 110, the cell proliferation rate and direction of proliferation in the first nanofiber layer 110 can be adjusted.

The first nanofiber layer 110 may be a highly degradable/highly hydrophilic biomaterial combined with PGA. Highly degradable/highly hydrophilic biomaterials may include synthetic materials such as PEO (Polyethylene Oxide), PEG (Polyethylene Glycol), PDO (Polydioxanone), PVP (Polyvinylpyrrolidone), PVA (Polyvinyl alcohol), etc., and natural materials such as collagen, It may contain chitosan, gelatin, algin, hyaluronic acid (HA), etc.

According to this embodiment, when a highly degradable/highly hydrophilic biomaterial is combined with the PGA of the first nanofiber layer 110, it becomes easier to deposit cells in the pores of the first nanofiber layer 110, so that the first nanofiber layer 110 Cell proliferation may be promoted.

In one embodiment, the first nanofiber layer 110 may include a protein that improves wound healing, such as Epidermal Growth Factor (EGF). If a wound healing enhancing protein is injected between the pores of the first nanofiber layer 110, a wound healing effect can be obtained during cell deposition.

In another embodiment, the first nanofiber layer 110 may contain antibiotics and antibacterial substances such as chitosan, lactoferrin, vitamins, and silver. When antibiotics and antibacterial substances are introduced into the pores of the first nanofiber layer 110, antibiotic and antibacterial effects can be obtained during cell deposition.

In another embodiment, the first nanofiber layer 110 may include hemostatic factors such as thrombin, fibrin, and calcium ions. If a hemostatic factor is injected between the pores of the first nanofiber layer 110, hemostasis and coagulation effects can be achieved during cell deposition.

The second nanofiber layer 120 is an anti-adhesion layer and may include polylactic acid (PLA) and polylactic-co-glycolic acid (PLGA), which have high hydrophobic properties. The second nanofiber layer 120 contains PLA and PLGA materials, which have greater hydrophobic properties than hydrophilic properties, so they are not easily biodegraded, and thus remain between tissues for a long time, preventing adhesion between tissues.

The second nanofiber layer 120 is electrospun on the first nanofiber layer 110 and may have a second porosity that is smaller than the first porosity. The diameter of the nanofibers, the density of the nanofibers, and the second porosity of the second nanofiber layer 120 can be adjusted by electrospinning.

The second porosity is a value expressed as a ratio of the pore volume between nanofibers to the volume of the entire nanofiber, and can have a larger value as the pores become larger. The secondary porosity can be close to 0% to make cell deposition difficult.

In one embodiment, the second nanofiber layer 120 may be bonded to the first nanofiber layer 110 using an electric iron or ultrasonic welder.

The first nanofiber layer 110 and the second nanofiber layer 120 are made of nanofibers and can maintain mechanical properties, thereby increasing the stability of the nanofibers and keeping the pores and shape constant even when manufactured in the form of non-woven fabric. and can sufficiently withstand the pressure of the wound area.

Here, the thickness of the nanofibers can be adjusted according to the diameter of the inlet of the electrospinning device where the mixture is spun, the speed at which the mixture is spun, voltage and electric field, the nature of the added polymer, and the composition and concentration of the mixture. The thickness of the nanofiber is preferably 0.001 to 10 ㎛, but is not limited thereto.

The bio-scaffold 100 with a three-dimensional structure using an electrospinning device according to this embodiment has a first nanofiber layer 110 in contact with the wound area to promote cell deposition and a first nanofiber layer 110 in contact between tissues to prevent adhesion between tissues. By forming the second nanofiber layer 120 integrally, it is possible to provide a wound covering function and an adhesion prevention function at the same time.

In addition, the three-dimensional bio-support 100 using an electrospinning device according to this embodiment is a first nanofiber layer 110 using electrospinning to be suitable for the cell attachment or non-attachment environment and the cell proliferation environment. The porosity and the second porosity of the second nanofiber layer 120 can be adjusted to promote cell proliferation in the nanofiber layer or to inhibit cell proliferation in the nanofiber layer.

In other words, the larger the porosity, the easier it is to deposit cells in the pores of the nanofiber layer, thus promoting cell proliferation in the nanofiber layer. The smaller the porosity, the more difficult it is to deposit cells in the pores of the nanofiber layer, so cell proliferation in the nanofiber layer may be suppressed. .

Figure 2 is an image to explain the multi-layer structure of the three-dimensional bio support 100 using an electrospinning device.

As a result of the test, it was confirmed that the cross-sectional thickness of the three-dimensional bio support 100 using an electrospinning device was 947 μm to 1093 μm (micrometer), and that it could be formed into a multilayer structure with an average of 1014 μm.

In order to maintain a uniform cross-sectional thickness of the three-dimensional bio support 100 using an electrospinning device, the spinning time and process conditions of the electrospinning device can be adjusted.

In addition, the degree of cell deposition and inhibition can be controlled by adjusting the porosity of each layer constituting the three-dimensional bio scaffold 100 using an electrospinning device.

Hereinafter, various examples of a three-dimensional bio-support formed in a multi-layer structure will be described in detail.

Figure 3 is a diagram schematically showing a cross section of a three-dimensional bio-support using an electrospinning device according to a second embodiment of the present invention.

As shown in FIG. 3, the three-dimensional bio support 200 using the electrospinning device according to this embodiment may include a first nanofiber layer 210 and a second nanofiber layer 220.

The first nanofiber layer 210 and the second nanofiber layer 220 according to this embodiment are the same as the configuration of the first nanofiber layer 110 and the second nanofiber layer 120 described in FIG. 1, so only for different configurations. Explain.

The first nanofiber layer 210 may include an 11th nanofiber layer 211, a 12th nanofiber layer 212, and a 13th nanofiber layer 213. Here, the first nanofiber layer 210 may be divided into an 11th nanofiber layer 211, a 12th nanofiber layer 212, and a 13th nanofiber layer 213 in the depth direction.

The 11th nanofiber layer 211 may be in contact with the second nanofiber layer 220. The 11th nanofiber layer 211 may have an 11th porosity 211a. For example, the 11th porosity 211a may be 83 to 85%.

The twelfth nanofiber layer 212 may be located between the eleventh nanofiber layer 211 and the thirteenth nanofiber layer 213. The twelfth nanofiber layer 212 may have a twelfth porosity 212a. For example, the twelfth porosity 212a may be 84 to 86%.

The 13th nanofiber layer 213 may be located at the outermost part of the first nanofiber layer 210. The thirteenth nanofiber layer 213 may have a thirteenth porosity 213a. For example, the thirteenth porosity 213a may be 85 to 87%.

According to the three-dimensional bio support 200 using an electrospinning device according to this embodiment, the 11th porosity 211a of the 11th nanofiber layer 211 is the 12th porosity 212a of the 12th nanofiber layer 212. The twelfth porosity 212a of the twelfth nanofiber layer 212 may be smaller than the thirteenth porosity 213a of the thirteenth nanofiber layer 213.

That is, according to this embodiment, the nanofibers of the three-dimensional bio scaffold 200 are patterned to have the largest porosity 213a in the 13th nanofiber layer 213 in contact with the wound area, and The nanofibers may be patterned to have a 12th porosity 212a and an 11th porosity 211a in each of the 12th nanofiber layer 212 and the 11th nanofiber layer 211 so that the porosity decreases in the direction away from.

Meanwhile, in Figure 3, the thickness of the 11th nanofiber layer 211, the 12th nanofiber layer 212, and the 13th nanofiber layer 213 is shown to be different, but they are not limited to this and can also be formed with the same thickness.

Figure 4 is an image for explaining the nanofiber diameter of the first nanofiber layer 210 of the three-dimensional bio support 200 using an electrospinning device according to the second embodiment of the present invention.

Figure 4 (a) is an enlarged view of the nanofibers of the 11th nanofiber layer 211, and may have a nanofiber diameter of 5 mm to 10 mm (millimeter). Figure 4 (b) is an enlarged view of the nanofibers of the twelfth nanofiber layer 212, and may have a nanofiber diameter of 800 μm to 5 mm (millimeter). Figure 4 (c) is an enlarged view of the nanofibers of the 13th nanofiber layer 213, and may have a nanofiber diameter of 200 μm to 600 μm (micrometer).

According to Figure 4, the porosity of the first nanofiber layer 210 can be adjusted depending on the diameter of the nanofiber. That is, the porosity of the first nanofiber layer 210 may decrease as the diameter of the nanofibers increases as the density increases, and as the diameter of the nanofibers decreases, the density decreases, so the porosity may increase.

Accordingly, the 12th porosity 212a of the 12th nanofiber layer 212 is greater than the 11th porosity 211a of the 11th nanofiber layer 211, and the 12th porosity 212a of the 12th nanofiber layer 212 is greater than the 11th porosity 211a of the 11th nanofiber layer 211. 13 The porosity 213a of the nanofiber layer 213 may be large.

Therefore, in the three-dimensional bio-support 200 according to this embodiment, the movement of cells is guided to the pores formed in the 13th nanofiber layer 213 having the largest porosity 213a, and the most cells are deposited. , the movement of cells is guided to the pores formed in the 12th nanofiber layer 212 having the middle 12th porosity 212a, so that fewer cells are deposited than the 13th nanofiber layer 213, and the smallest 11th porosity 211a The movement of cells is guided by the pores formed in the 11th nanofiber layer 211, so that cells can be deposited in a state in which there are fewer or almost no cells than in the 12th nanofiber layer 212.

Referring to [Table 1] below, the porosity test results of the nanofiber layer showed that the smaller the density, the larger the porosity. Accordingly, test product name 1 with the largest porosity may be the 13th nanofiber layer (213), test product name 2 with a medium porosity may be the 12th nanofiber layer (212), and test product name 3 with the smallest porosity may be the 11th nanofiber layer. It may be a fibrous layer (211).

Test product name Analysis items unit Analysis One Apparent density g/mL 0.10 porosity % 86.4 2 Apparent density g/mL 0.11 porosity % 83.4 3 Apparent density g/mL 0.13 porosity % 83.0

Figure 5 is an enlarged view of the nanofibers of the second nanofiber layer 220. The second nanofiber layer 220 is thicker than the nanofiber diameter of the first nanofiber layer 210 in Figure 4, resulting in increased density and decreased porosity. You can see that

That is, the second nanofiber layer 220 may have a porosity close to 0%, and thus cell proliferation may be suppressed.

According to this embodiment, the movement path and speed of cell deposition vary based on the pattern of the nanofibers according to the porosity of the nanofiber layer, and depending on the movement path and speed of cell deposition, cell proliferation is promoted in the nanofiber layer or in the nanofiber layer. Cell proliferation can be inhibited.

Figure 6 is a diagram schematically showing a cross section of a three-dimensional bio support 300 using an electrospinning device according to a third embodiment of the present invention.

As shown in FIG. 6, the three-dimensional bio support 300 using the electrospinning device according to this embodiment may include a first nanofiber layer 310 and a second nanofiber layer 320.

The first nanofiber layer 310 and the second nanofiber layer 320 according to this embodiment are the same as the configuration of the first nanofiber layer 110 and the second nanofiber layer 120 described in FIG. 1, so only for different configurations. Explain.

The first nanofiber layer 310 may include a first nanofiber support part 311, a second nanofiber support part 312, and a nanofiber pattern part 313. Here, the first nanofiber layer 310 may be divided into a first nanofiber support part 311, a second nanofiber support part 312, and a nanofiber pattern part 313 in the direction of the contact surface of the wound area.

The first nanofiber support 311 is located on the outer portion of the wound area and may have a first support porosity 311a. The first support porosity 311a may be smaller than the pattern porosity 313a of the nanofiber pattern portion 313, and thus cell deposition may be suppressed in the first nanofiber support portion 311 rather than in the nanofiber pattern portion 313. You can.

In other words, the first nanofiber support part 311 may play a role in suppressing cell deposition and supporting the nanofiber pattern part 313 located corresponding to the wound area to prevent it from falling off the wound area. The first nanofiber support 311 may include a PGA material with excellent adhesion and high hydrophilic properties.

The second nanofiber support 312 is located on the outer portion of the wound area and may have a second support porosity 312a. The second support porosity 312a may be smaller than the pattern porosity 313a of the nanofiber pattern portion 313, and thus cell deposition may be suppressed in the second nanofiber support portion 312 rather than in the nanofiber pattern portion 313. You can.

In other words, the second nanofiber support part 312 may play a role in suppressing cell deposition and preventing the nanofiber pattern part 313 located corresponding to the wound area from falling off the wound area. The second nanofiber support portion 312 may include a PGA material with excellent adhesion and high hydrophilic properties.

Meanwhile, the first support porosity 311a of the first nanofiber support 311 and the second support porosity 312a of the second nanofiber support 312 may have the same porosity, but are not intended to be limited thereto.

The nanofiber pattern portion 313 is located corresponding to the wound area and may be located between the first nanofiber support portion 311 and the second nanofiber support portion 312.

The nanofiber pattern portion 313 may have a pattern porosity 313a. The pattern porosity 313a may be greater than the first support porosity 311a of the first nanofiber support 311 and the second support porosity 312a of the second nanofiber support 312. For example, the pattern porosity 313a may be 85 to 87%.

That is, the nanofiber pattern portion 313 may have greater hydrophilicity than the first nanofiber support portion 311 and the second nanofiber support portion 312.

According to the three-dimensional bio support 300 using an electrospinning device according to this embodiment, nanofibers are patterned with a pattern porosity 313a in the nanofiber pattern portion 313, thereby guiding the movement of cells into the pores. Cell growth may be promoted around the wound area.

In addition, according to the three-dimensional bio scaffold 300 using an electrospinning device according to this embodiment, cell growth is promoted at the wound site according to the pattern porosity 313a of the nanofiber pattern portion 313, which has a large hydrophilic property. On the outskirts of the wound area, the first nanofiber support part 311 and the second nanofiber support part 312, which have less hydrophilic properties, support the nanofiber pattern part 313 and adhere to the cells, forming a nanofiber pattern without falling off from the cells. Part 313 can be continuously positioned at the wound site.

Figure 7 is a diagram schematically showing a cross section of a three-dimensional bio support 400 using an electrospinning device according to a fourth embodiment of the present invention.

As shown in FIG. 4, the three-dimensional bio support 400 using the electrospinning device according to this embodiment may include a first nanofiber layer 410 and a second nanofiber layer 420.

The first nanofiber layer 410 and the second nanofiber layer 420 according to this embodiment are the same as the configuration of the first nanofiber layer 110 and the second nanofiber layer 120 described in FIG. 1, so only for different configurations. Explain.

The first nanofiber layer 410 may include a first nanofiber support portion 411, a second nanofiber support portion 412, and a nanofiber pattern portion 413.

The nanofiber pattern portion 413 may have a pattern porosity 413a to correspond to the wound area. The nanofiber pattern portion 413 may have nanofibers patterned to have a pattern porosity 413a. In the nanofiber pattern portion 413, pores patterned according to the shape of the wound are formed, allowing concentrated cell deposition in the shape of the wound.

The first nanofiber support 411 is located on the outer portion of the wound area and may have a first support porosity 411a. The first support porosity 411a may be smaller than the pattern porosity 413a of the nanofiber pattern portion 413, and thus cell deposition may be suppressed in the first nanofiber support portion 411 rather than in the nanofiber pattern portion 413. You can.

In other words, the first nanofiber support part 411 may play a role in suppressing cell deposition and supporting the nanofiber pattern part 413 located corresponding to the wound area so that it does not fall off the wound area. The first nanofiber support portion 411 may include a PGA material with excellent adhesion and high hydrophilic properties.

The second nanofiber support 412 is located on the outer portion of the wound area and may have a second support porosity 412a. The second support porosity 412a may be smaller than the pattern porosity 413a of the nanofiber pattern portion 413, and thus cell deposition may be suppressed in the second nanofiber support portion 412 rather than in the nanofiber pattern portion 413. You can.

In other words, the second nanofiber support portion 412 may play a role in suppressing cell deposition and preventing the nanofiber pattern portion 413 located corresponding to the wound site from falling off the wound site. The second nanofiber support portion 412 may include a PGA material with excellent adhesion and high hydrophilic properties.

Meanwhile, the first support porosity 411a of the first nanofiber support 411 and the second support porosity 412a of the second nanofiber support 412 may have the same porosity, but are not intended to be limited thereto.

According to the three-dimensional bio scaffold 400 using an electrospinning device according to this embodiment, the nanofibers are patterned to have a patterned porosity 413a in a shape and position corresponding to the wound area, thereby facilitating the movement of cells into the pores. By focusing the guidance, cell growth can be promoted around the wound area.

Figure 8 is a diagram schematically showing a cross section of a three-dimensional bio support 500 using an electrospinning device according to the fifth embodiment of the present invention.

As shown in FIG. 8, the three-dimensional bio support 500 using an electrospinning device according to this embodiment includes a first nanofiber layer 510, a second nanofiber layer 520, and a hydrophobic coating layer 530. It can be included.

The first nanofiber layer 510 and the second nanofiber layer 520 according to this embodiment are the same as the first nanofiber layer 110 and the second nanofiber layer 120 described in FIG. 1, so only the different configurations are used. Explain.

The hydrophobic coating layer 530 may be coupled to the second nanofiber layer 520. The hydrophobic coating layer 530 may be in the form of a film and have a porosity of 0%.

The hydrophobic coating layer 530 may include a material with very high hydrophobic properties.

[Table 2] below shows the results of measuring the helium leakage amount of the second nanofiber layer 530 and the helium leakage amount after combining the hydrophobic coating layer 530 to the second nanofiber layer 530.

Referring to [Table 2], it can be seen that the amount of helium leakage was significantly reduced in the second nanofiber layer 530 combined with the hydrophobic coating layer 530 under the same experimental conditions.

Test product name Helium leakage before coating (cm2/min) Helium leakage after coating (cm2/min) One 599 61 2 635 2 3 626 28 4 644 One 5 609 225

According to the three-dimensional bio support 500 using an electrospinning device according to this embodiment, a hydrophobic coating layer 530 with a porosity close to 0% is combined with the second nanofiber layer 530 to block the inflow of air and prevent cells from entering. The proliferation of is suppressed, and thus the anti-adhesion effect can be maximized. Although the above has been described with reference to an embodiment of the present invention, those skilled in the art will understand that the present invention described in the patent claims below It will be understood that the present invention can be modified and changed in various ways without departing from the spirit and scope of the invention.

100, 200, 300, 400, 500: 3D structured bio support using electrospinning device
110, 210, 310, 410, 510: first nanofiber layer
120, 220, 320, 420, 520: second nanofiber layer
211: 11th nanofiber layer 212: 12th nanofiber layer
213: 13th nanofiber layer 311, 411: 1st nanofiber support part
312, 412: second nanofiber support portion 313, 413: nanofiber pattern portion
530: Hydrophobic coating layer

Claims (6)

A first nanofiber layer that is manufactured by electrospinning, has a first porosity, and is a wound coating layer containing polyglycolic acid (PGA); and
Adhesion comprising PLA (Polylactic Acid) and PLGA (Polylactic-co-glycolic Acid), which are electrospun on the first nanofiber layer and have a second porosity smaller than the first porosity and have a slower decomposition rate than the PGA (Polyglycolic acid) It includes a second nanofiber layer that is an anti-inflammatory layer,
The first nanofiber layer is an 11th nanofiber layer in contact with the second nanofiber layer, a 13th nanofiber layer located on the outermost side of the first nanofiber layer, and a 13th nanofiber layer located between the 11th nanofiber layer and the 13th nanofiber layer. It includes a twelfth nanofiber layer,
The 11th porosity of the 11th nanofiber layer is smaller than the 12th porosity of the 12th nanofiber layer, and the 12th porosity of the 12th nanofiber layer is smaller than the 13th porosity of the 13th nanofiber layer. Three-dimensional using an electrospinning device Structural bio-scaffold. delete delete According to paragraph 1,
The first nanofiber layer is,
A first nanofiber support part that contacts the outer part of the wound area and has a first support porosity, a nanofiber pattern part that extends from the first nanofiber support part and contacts the wound part and has a pattern porosity, and the nanofiber pattern part. a second nanofiber support extending and contacting another outer portion of the wound site and having a second support porosity;
The wound is formed in a pattern of a certain shape formed by a plurality of pores in the nanofiber pattern portion having a pattern porosity greater than the first support porosity of the first nanofiber support portion and the second support porosity of the second nanofiber support portion. A three-dimensional bio scaffold using an electrospinning device in which cells in the area are guided and proliferated, and the first nanofiber support part and the second nanofiber support part support the nanofiber pattern part to be positioned at the wound site. delete According to paragraph 1,
A three-dimensional bio support using an electrospinning device further comprising a hydrophobic coating layer bonded to the second nanofiber layer.