KR102584680B1 - Method for manufacturing tea of Lentinula edodes tea improved physiological activities and nutrition components - Google Patents

Method for manufacturing tea of Lentinula edodes tea improved physiological activities and nutrition components Download PDF

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KR102584680B1
KR102584680B1 KR1020200177649A KR20200177649A KR102584680B1 KR 102584680 B1 KR102584680 B1 KR 102584680B1 KR 1020200177649 A KR1020200177649 A KR 1020200177649A KR 20200177649 A KR20200177649 A KR 20200177649A KR 102584680 B1 KR102584680 B1 KR 102584680B1
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shiitake
shiitake mushroom
tea
mushroom tea
mushrooms
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KR20220087185A (en
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안기홍
조재한
한재구
김옥태
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대한민국
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/06Treating tea before extraction; Preparations produced thereby
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/06Treating tea before extraction; Preparations produced thereby
    • A23F3/14Tea preparations, e.g. using additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/34Tea substitutes, e.g. matè; Extracts or infusions thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/40Tea flavour; Tea oil; Flavouring of tea or tea extract
    • A23F3/405Flavouring with flavours other than natural tea flavour or tea oil
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D85/00Containers, packaging elements or packages, specially adapted for particular articles or materials
    • B65D85/70Containers, packaging elements or packages, specially adapted for particular articles or materials for materials not otherwise provided for
    • B65D85/804Disposable containers or packages with contents which are mixed, infused or dissolved in situ, i.e. without having been previously removed from the package
    • B65D85/808Disposable containers or packages with contents which are mixed, infused or dissolved in situ, i.e. without having been previously removed from the package for immersion in the liquid to release part or all of their contents, e.g. tea bags
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F2200/00Special features
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00Processes
    • A23V2300/10Drying, dehydrating
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00Processes
    • A23V2300/24Heat, thermal treatment
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00Processes
    • A23V2300/31Mechanical treatment

Abstract

본 발명은 생리활성 및 영양성분이 증가된 표고버섯차 제조방법에 관한 것으로, 본 발명의 표고버섯차는 표고버섯 및 곡물류를 혼합하여 이들의 조성 및 배합비에 따른 항산화 활성, 아질산염 소거능, 폴리페놀 함량, 베타글루칸 함량, 아미노산 함량을 확인함으로써, 생리활성 및 영양성분이 증진된 최적 조성 및 배합비를 도출하였으며, 이를 통해 제조된 표고버섯차는 생리활성 및 영양성분이 증진된 효과를 가진다.The present invention relates to a method for producing shiitake mushroom tea with increased physiological activity and nutritional components. The shiitake mushroom tea of the present invention is made by mixing shiitake mushrooms and grains, and according to their composition and mixing ratio, the antioxidant activity, nitrite scavenging ability, polyphenol content, By checking the beta-glucan content and amino acid content, the optimal composition and mixing ratio with improved physiological activity and nutrients were derived, and the shiitake mushroom tea prepared through this has the effect of improving physiological activity and nutritional ingredients.

Description

생리활성 및 영양성분이 증가된 표고버섯차 제조방법 {Method for manufacturing tea of Lentinula edodes tea improved physiological activities and nutrition components}Method for manufacturing shiitake mushroom tea with improved physiological activities and nutritional components {Method for manufacturing tea of Lentinula edodes tea improved physiological activities and nutrition components}

본 발명은 생리활성 및 영양성분이 증가된 표고버섯차 제조방법에 관한 것이다.The present invention relates to a method for producing shiitake mushroom tea with increased physiological activity and nutritional components.

버섯은 독특한 맛과 향을 지니고 있으며 영양학적으로 필수 아미노산, 단백질, 무기질과 같은 중요한 영양성분을 다량 함유하고 있다. 더 나아가 약리적으로 노화, 암 등의 원인이 되는 활성산소의 산화반응 및 라디컬의 반응성을 억제하여 산화 스트레스로부터 인체를 보호하는 항산화 물질을 비롯하여 면역활성체의 기능 및 항균, 항바이러스, 항종양 효과가 있는 다량의 베타글루칸이 함유하고 있다고 보고되고 있다 (Cho et al., 2014; Choi et al., 2010; Barros et al., 2007; Han et al., 2015; Manzi et al., 2001; Qi et al., 2013). 이처럼 최근 생활수준과 소득수준의 향상과 더불어 삶의 질 향상에 관한 관심이 꾸준히 증가됨에 따라 버섯의 다양한 생리활성 성분의 이용과 관심이 점차 증가되고 있는 실정이다 (Park et al., 1995; Song et al., 2003).Mushrooms have a unique taste and aroma and contain a large amount of nutritionally important nutrients such as essential amino acids, proteins, and minerals. Furthermore, it contains antioxidant substances that protect the body from oxidative stress by pharmacologically inhibiting the oxidation reaction of oxygen radicals and the reactivity of radicals that cause aging, cancer, etc., as well as the function of immune activators and antibacterial, antiviral, and antitumor effects. It is reported to contain a large amount of beta-glucan (Cho et al., 2014; Choi et al., 2010; Barros et al., 2007; Han et al., 2015; Manzi et al., 2001; Qi et al., 2013). As interest in improving the quality of life has steadily increased along with recent improvements in living standards and income levels, the use and interest in various bioactive components of mushrooms is gradually increasing (Park et al., 1995; Song et al., 1995). al., 2003).

국내 버섯산업은 생산량이 수요와 공급에 비하여 많아지고 수출둔화에 따른 가격하락과 더불어 내수시장이 상당한 수준 충족되어 포화상태에 이르렀으며 그로 인하여 농가수익성은 점차 악화되고 있는 실정이다 (Chang, 2008; Lee and Seo, 2005). 더 나아가 국내에서 소비되고 있는 버섯 중 90% 이상이 큰느타리, 느타리, 팽이, 양송이, 표고가 차지하고 있어 품목편중 현상이 심화되고 있다 (Lee et al., 2017). 버섯산업의 현황과 개선과제에 대한 분석을 행한 Yeom et al. (2019)의 보고에 의하면 계층적 분석방법 (AHP) 결과, 버섯산업의 부문별 우선순위는 수출 및 마케팅부문이 1위, 가공부문이 2위로 분석되었으며, 소비자 선호 조사를 통한 기능성 버섯품종 개발 등 다양한 품종의 개발 및 생산과 국내외 소비자 특성과 트랜드에 맞는 신상품 제품개발의 필요성을 강조하였다. 또한 Lee and Seo, (2005)는 버섯산업의 발전방향으로 내수시장이 형성되어 수요를 충족시켜 고부가가치 상품을 개발하고 수출하여 단순한 버섯만이 아니라 버섯을 중심으로 한 전후방 산업까지 함께 발전하여야 한다고 하였다. 이처럼 국내 버섯산업 불황의 악순환을 극복하기 위해서는 생버섯 시장 이외에도 소비자들의 소비 욕구에 맞는 버섯품종 개발과 함께 다양한 가공제품의 개발이 절실하다.The domestic mushroom industry has reached a saturation point as production volume has increased compared to supply and demand, prices have fallen due to export slowdown, and the domestic market has been met to a significant level. As a result, farm profitability is gradually worsening (Chang, 2008; Lee) and Seo, 2005). Furthermore, more than 90% of the mushrooms consumed in Korea are large oysters, oysters, enokis, button mushrooms, and shiitake mushrooms, which is worsening the product concentration phenomenon (Lee et al., 2017). Yeom et al. analyzed the current status and improvement tasks of the mushroom industry. (2019), as a result of the Hierarchical Analysis Method (AHP), the priorities for each sector of the mushroom industry were analyzed to be 1st in the export and marketing sector and 2nd in the processing sector, and development of functional mushroom varieties through consumer preference surveys, etc. The need to develop and produce various varieties and develop new products that fit domestic and international consumer characteristics and trends was emphasized. In addition, Lee and Seo, (2005) stated that the development direction of the mushroom industry should be to form a domestic market to meet demand, develop and export high value-added products, and develop not only mushrooms but also forward and backward industries centered on mushrooms. . In order to overcome this vicious cycle of recession in the domestic mushroom industry, the development of various processed products as well as the development of mushroom varieties that meet the consumption needs of consumers in addition to the fresh mushroom market is urgently needed.

버섯의 여러 가지 유익한 효능들이 알려지면서 건강기능식품으로 활용도가 높다고 할 수 있다. 하지만 버섯 소비의 대부분이 가정과 식당에 국한되어 있는 것으로 보아 아직까지는 생버섯을 이용한 요리에 주로 이용되고 있다고 판단된다 (Jo et al., 2009). 그 이외에 숙취해소음료, 빵, 면류, 비스켓 등의 기능성 버섯이 식품원료 및 일반식품류에 활용되어 여러 특허와 가공제품들이 개발되어 오고 있다 (Park, 2008). 버섯 가공품 개발과정 중 1차 가공에서 생산된 건조버섯 분말을 이용하여 커피, 차, 조미료, 고형 육수첨가물 등에 혼합하여 섭취할 경우 버섯의 기능성 성분들도 함께 섭취할 수 있다.As the various beneficial effects of mushrooms become known, they can be said to be highly utilized as health functional foods. However, since most mushroom consumption appears to be limited to homes and restaurants, it is believed that raw mushrooms are still mainly used in cooking (Jo et al., 2009). In addition, functional mushrooms such as hangover relief drinks, bread, noodles, and biscuits have been used as food ingredients and general foods, and several patents and processed products have been developed (Park, 2008). If you use the dried mushroom powder produced in the first processing during the development of processed mushroom products and mix it with coffee, tea, seasoning, solid broth additives, etc., you can also consume the functional ingredients of the mushroom.

표고버섯 (Lentinula edodes)은 담자균류 주름버섯목 낙엽버섯과에 속하는 버섯으로서, 영양기관인 균사체와 번식기관인 포자를 지닌 자실체로 되어 있고, 뒷면이 하얗고 깨끗한 주름이 있으며, 특별한 향미와 감칠맛이 있어 예로부터 식용으로 널리 이용된 식물이다.Shiitake mushroom ( Lentinula edodes ) is a mushroom belonging to the deciduous mushroom family of the order Basidiomycetes. It consists of a fruiting body with mycelium as a nutritional organ and spores as a reproductive organ. It has white and clean wrinkles on the back, and has a special flavor and umami, so it has been used since ancient times. It is a plant widely used for food.

표고버섯에는 단백질, 지방, 탄수화물 뿐만 아니라 칼슘, 칼륨, 인, 셀레늄, 철분 등의 미네랄, B1, B2 등의 비타민, 식이섬유가 풍부하게 함유되어 있다. 또한, 프로비타민 D(provitamin D)인 에르고스테롤(ergosterol), 혈압 강하와 당뇨병에 효과적인 에리타데닌(erithadenine), 탄수화물ㆍ지방ㆍ단백질 대사 및 세포호흡과 당 분해 작용에 관여하는 나이아신(niacin) 등 생리활성물질이 있어 식용 외에 약재로도 이용되었다.Shiitake mushrooms are rich in not only protein, fat, and carbohydrates, but also minerals such as calcium, potassium, phosphorus, selenium, and iron, vitamins such as B1 and B2, and dietary fiber. In addition, ergosterol, which is provitamin D, eritadenine, which is effective in lowering blood pressure and treating diabetes, and niacin, which is involved in carbohydrate, fat, and protein metabolism, cellular respiration, and glycolysis, etc. Because it contains physiologically active substances, it is used not only as food but also as a medicine.

또한, 표고버섯은 건조 시 레타닌 성분이 함유되어 있어 비장을 강화시켜주며 면역력을 높여주고 암세포가 전이하는 것을 방지해주는 효능이 있으며, 비타민D가 풍부하여 콜레스테롤의 합성을 억제하고 배출하는데 도움이 되기 때문에 고혈압, 고지혈증, 당뇨, 성인병 등 다양한 효능이 있다.In addition, shiitake mushrooms contain retanin when dried, which strengthens the spleen, increases immunity, and prevents cancer cells from metastasizing. They are rich in vitamin D, which helps suppress the synthesis and excretion of cholesterol. Therefore, it has various effects such as high blood pressure, hyperlipidemia, diabetes, and adult diseases.

이에, 본 발명자들은 표고버섯 및 곡물류를 포함하여 맛과 기능성이 우수한 음료를 개발하여 상품화하고자 하였으며, 그 결과 표고버섯 및 곡물류를 특정 조성비로 배합하고, 특정 침지 조건으로 차를 제조하면 생리활성 및 영양성분이 증진되는 것을 확인함으로써 본 발명을 완성하였다.Accordingly, the present inventors attempted to develop and commercialize a beverage containing shiitake mushrooms and grains with excellent taste and functionality. As a result, when shiitake mushrooms and grains were mixed in a specific composition ratio and tea was manufactured under specific steeping conditions, the physiological activity and nutrition were improved. The present invention was completed by confirming that the components were improved.

대한민국 등록특허 제10-1584060호Republic of Korea Patent No. 10-1584060

본 발명의 목적은 생리활성과 영양성분이 증진된 표고버섯차 제조방법을 제공하는 데 있다.The purpose of the present invention is to provide a method for producing shiitake mushroom tea with improved physiological activity and nutritional components.

또한, 본 발명의 다른 목적은 상기 방법으로 제조된 표고버섯차를 제공하는 데 있다.In addition, another object of the present invention is to provide shiitake mushroom tea prepared by the above method.

상기와 같은 목적을 달성하기 위해, 본 발명은 표고버섯의 생시료를 칼날회전수 1,100 내지 1,300 rpm/min으로 1분 내지 2분 동안 6 내지 8mm 크기로 세절하는 단계; 온수를 이용한 대류식 열풍건조기를 이용하여 1차 온도조건으로 건조기내 40 내지 50℃, 온수온도 50 내지 60℃의 조건으로 버섯을 25 내지 35시간 동안 건조하는 단계; 2차 온도조건으로 건조기내 55 내지 65℃, 온수온도 65 내지 75℃의 조건으로 버섯을 11 내지 13시간 동안 건조하는 단계; 상기 건조 단계 후 수분함량을 측정하여 수분율 1 내지 7%까지 추가적으로 건조시키는 단계; 로스팅 기기에 상기 추가 건조 단계 후의 건조된 표고버섯을 넣고 75℃에서 드럼을 회전시키는 예열 단계; 90 내지 110℃에서 4 내지 6분간 1차로 로스팅 처리하는 단계; 120 내지 140℃에서 4 내지 6분간 2차로 로스팅 처리하는 단계; 160 내지 180℃에서 4 내지 6분간 3차로 로스팅 처리하는 단계; 로스팅처리가 완료되는 즉시 냉각하여 상온까지 온도를 하강시키는 단계; 현미 또는 귀리를 210 내지 220℃에서 5 분간 로스팅 처리하는 단계; 및 상기 로스팅된 표고버섯과 로스팅된 현미 또는 귀리를 혼합하여 티백 (Tea bag)으로 제조하는 단계;를 포함하는 생리활성과 영양성분이 증진된 표고버섯차 제조방법을 제공한다.In order to achieve the above object, the present invention includes the steps of chopping a raw sample of shiitake mushrooms into pieces of 6 to 8 mm in size for 1 to 2 minutes at a blade rotation speed of 1,100 to 1,300 rpm/min; Drying mushrooms for 25 to 35 hours using a convection hot air dryer using hot water at a primary temperature of 40 to 50°C in the dryer and a hot water temperature of 50 to 60°C; Drying the mushrooms for 11 to 13 hours in a dryer at a secondary temperature of 55 to 65°C and a hot water temperature of 65 to 75°C; After the drying step, measuring the moisture content and additionally drying to a moisture content of 1 to 7%; A preheating step of placing dried shiitake mushrooms after the additional drying step in a roasting device and rotating the drum at 75°C; Primary roasting at 90 to 110°C for 4 to 6 minutes; Secondary roasting at 120 to 140°C for 4 to 6 minutes; Roasting three times at 160 to 180°C for 4 to 6 minutes; Cooling immediately after the roasting process is completed to lower the temperature to room temperature; Roasting brown rice or oats at 210 to 220°C for 5 minutes; and mixing the roasted shiitake mushrooms with roasted brown rice or oats to produce a tea bag. It provides a method for producing shiitake mushroom tea with improved physiological activity and nutritional components, including the step of mixing the roasted shiitake mushrooms with the roasted brown rice or oats.

이어서, 본 발명은 상기 방법으로 제조된 표고버섯차를 제공한다.Next, the present invention provides shiitake mushroom tea prepared by the above method.

본 발명은 표고버섯 및 곡물류를 최적 배합비로 혼합함으로써 생리활성 (항산화 활성 및 아질산염 소거능) 및 영양성분 (폴리페놀, 베타글루칸, 아미노산) 함량이 증진된 표고버섯차에 관한 것으로, 표고버섯 및 곡물류를 혼합하여 이들의 조성 및 배합비에 따른 항산화 활성, 아질산염 소거능, 폴리페놀 함량, 베타글루칸 함량, 아미노산 함량을 확인함으로써, 생리활성 및 영양성분이 증진된 최적 조성 및 배합비를 도출하였으며, 이를 통해 제조된 표고버섯차는 생리활성 및 영양성분이 증진된 효과를 가진다.The present invention relates to shiitake mushroom tea with improved physiological activity (antioxidant activity and nitrite scavenging ability) and nutritional content (polyphenol, beta-glucan, amino acid) by mixing shiitake mushrooms and grains at an optimal mixing ratio. By mixing them and checking their antioxidant activity, nitrite scavenging ability, polyphenol content, beta-glucan content, and amino acid content according to their composition and mixing ratio, the optimal composition and mixing ratio with improved physiological activity and nutrients were derived, and the shiitake prepared through this was derived. Mushroom tea has the effect of enhancing physiological activity and nutrients.

도 1은 곡물첨가 표고버섯차의 추출온도 및 시간별 아질산염 소거능 확인 결과를 나타낸 그래프이다.
도 2는 곡물첨가 표고버섯차의 추출온도 및 시간별 DPPH 라디컬 소거능 확인 결과를 나타낸 그래프이다.
Figure 1 is a graph showing the results of confirming the nitrite scavenging ability of grain-added shiitake mushroom tea by extraction temperature and time.
Figure 2 is a graph showing the results of confirming the DPPH radical scavenging ability of grain-added shiitake mushroom tea by extraction temperature and time.

이하, 첨부된 도면을 참조하여 본 발명의 구현예로 본 발명을 상세히 설명하기로 한다. 다만, 하기 구현 예는 본 발명에 대한 예시로 제시되는 것으로, 당업자에게 주지 저명한 기술 또는 구성에 대한 구체적인 설명이 본 발명의 요지를 불필요하게 흐릴 수 있다고 판단되는 경우에는 그 상세한 설명을 생략할 수 있고, 이에 의해 본 발명이 제한되지는 않는다. 본 발명은 후술하는 특허 청구범위의 기재 및 그로부터 해석되는 균등 범주 내에서 다양한 변형 및 응용이 가능하다.Hereinafter, the present invention will be described in detail through embodiments of the present invention with reference to the attached drawings. However, the following implementation examples are provided as examples of the present invention, and if it is judged that a detailed description of a technology or configuration well known to those skilled in the art may unnecessarily obscure the gist of the present invention, the detailed description may be omitted. , the present invention is not limited thereby. The present invention is capable of various modifications and applications within the description of the patent claims described below and the scope of equivalents interpreted therefrom.

또한, 본 명세서에서 사용되는 용어 (terminology)들은 본 발명의 바람직한 실시 예를 적절히 표현하기 위해 사용된 용어들로서, 이는 사용자, 운용자의 의도 또는 본 발명이 속하는 분야의 관례 등에 따라 달라질 수 있다. 따라서 본 용어들에 대한 정의는 본 명세서 전반에 걸친 내용을 토대로 내려져야 할 것이다. 명세서 전체에서, 어떤 부분이 어떤 구성요소를 “포함”한다고 할 때, 이는 특별히 반대되는 기재가 없는 한 다른 구성요소를 제외하는 것이 아니라 다른 구성 요소를 더 포함할 수 있는 것을 의미한다.In addition, the terminology used in this specification is a term used to appropriately express preferred embodiments of the present invention, and may vary depending on the intention of the user or operator or the customs of the field to which the present invention belongs. Therefore, definitions of these terms should be made based on the content throughout this specification. Throughout the specification, when it is said that a part “includes” a certain element, this means that it may further include other elements rather than excluding other elements, unless specifically stated to the contrary.

본 명세서 전체에 걸쳐, 특정 물질의 농도를 나타내기 위하여 사용되는 '%'는 별도의 언급이 없는 경우, 고체/고체는 (w/w) %, 고체/액체는 (w/v) %, 그리고 액체/액체는 (v/v) %이다.Throughout this specification, '%' used to indicate the concentration of a specific substance means (w/w) % for solid/solid, (w/v) % for solid/liquid, and Liquid/Liquid is (v/v) %.

본 발명은 로스팅 기기에 건조된 표고버섯을 넣고 90 내지 180℃에서 4 내지 6분간 로스팅 처리하는 1단계; 현미 또는 귀리를 210 내지 220℃에서 5분간 로스팅 처리하는 2단계; 및 상기 로스팅 처리된 표고버섯과 로스팅 처리된 현미 또는 귀리를 혼합하여 티백 (Tea bag)으로 제조하는 3단계;를 포함하는 생리활성과 영양성분이 증진된 표고버섯차 제조방법을 제공한다.The present invention includes the first step of placing dried shiitake mushrooms in a roasting machine and roasting them at 90 to 180°C for 4 to 6 minutes; Step 2: roasting brown rice or oats at 210 to 220°C for 5 minutes; and a third step of mixing the roasted shiitake mushrooms with the roasted brown rice or oats to produce a tea bag.

구체적으로, 상기 생리활성과 영양성분이 증진된 표고버섯차는Specifically, shiitake mushroom tea with improved physiological activity and nutritional components

a) 표고버섯의 생시료를 바람직하게는 칼날회전수 1,100 내지 1,300 rpm/min으로 1분 내지 2분 동안 6 내지 8mm 크기, 가장 바람직하게는 칼날회전수 1,200 rpm/min 으로 2분 동안 7mm 크기로 세절하는 단계;a) Raw samples of shiitake mushrooms are preferably cut to a size of 6 to 8 mm for 1 to 2 minutes at a blade rotation speed of 1,100 to 1,300 rpm/min, and most preferably to a size of 7 mm for 2 minutes at a blade rotation speed of 1,200 rpm/min. Chopping step;

b) 온수를 이용한 대류식 열풍건조기를 이용하여 바람직하게는 1차 온도조건으로 건조기내 40 내지 50℃, 온수온도 50 내지 60℃의 조건으로 25 내지 35시간 동안, 가장 바람직하게는 1차 온도조건으로 건조기내 45°C, 온수온도 55°C의 조건으로 30시간 동안 버섯을 건조하는 단계;b) Using a convection hot air dryer using hot water, preferably at a primary temperature of 40 to 50°C in the dryer and a hot water temperature of 50 to 60°C for 25 to 35 hours, most preferably as a primary temperature condition. Drying the mushrooms for 30 hours at 45°C in the dryer and 55°C hot water temperature;

c) 바람직하게는 2차 온도조건으로 건조기내 55 내지 65℃, 온수온도 65 내지 75℃의 조건으로 11 내지 13시간 동안, 가장 바람직하게는 2차 온도조건으로 건조기내 60°C, 온수온도 70°C의 조건으로 12시간 동안 버섯을 건조하는 단계;c) Preferably the secondary temperature conditions are 55 to 65°C in the dryer and the hot water temperature is 65 to 75°C for 11 to 13 hours, most preferably the secondary temperature conditions are 60°C in the dryer and the hot water temperature is 70°C. Drying the mushrooms for 12 hours at °C;

d) 상기 c)단계 후에 수분함량을 측정하여 수분율 1 내지 7%까지 추가적으로 건조시키는 단계;d) measuring the moisture content after step c) and additionally drying to a moisture content of 1 to 7%;

e) 로스팅 기기에 상기 d) 단계 후의 건조된 표고버섯을 넣고 바람직하게는 70 내지 80℃, 가장 바람직하게는 75℃에서 드럼을 회전시키는 예열 단계;e) a preheating step of putting the dried shiitake mushrooms from step d) into the roasting machine and rotating the drum at preferably 70 to 80°C, most preferably 75°C;

f) 바람직하게는 90 내지 110℃에서 4 내지 6분간, 가장 바람직하게는 100°C에서 5분간 1차로 로스팅 처리하는 단계; f) primary roasting, preferably at 90 to 110°C for 4 to 6 minutes, most preferably at 100°C for 5 minutes;

g) 바람직하게는 120 내지 140℃에서 4 내지 6분간, 가장 바람직하게는 130°C에서 5분간 2차로 로스팅 처리하는 단계; g) secondary roasting, preferably at 120 to 140°C for 4 to 6 minutes, most preferably at 130°C for 5 minutes;

h) 바람직하게는 160 내지 180℃에서 4 내지 6분간, 가장 바람직하게는 170°C에서 5분간 3차로 로스팅 처리하는 단계; h) third roasting, preferably at 160 to 180°C for 4 to 6 minutes, most preferably at 170°C for 5 minutes;

i) 로스팅처리가 완료되는 즉시 냉각하여 상온까지 온도를 하강시키는 단계;i) cooling immediately after the roasting process is completed to lower the temperature to room temperature;

k) 현미 또는 귀리를 바람직하게는 210 내지 220℃에서 4 내지 6분간, 가장 바람직하게는 200℃에서 5분간 로스팅 처리하는 단계; 및k) roasting brown rice or oats, preferably at 210 to 220° C. for 4 to 6 minutes, most preferably at 200° C. for 5 minutes; and

j) 상기 i) 단계의 표고버섯과 k) 단계의 현미 또는 귀리를 혼합하여 티백 (Tea bag)으로 제조하는 단계;를 거쳐 제조된 것일 수 있다.j) mixing the shiitake mushrooms in step i) and the brown rice or oats in step k) to produce a tea bag.

이어서, 본 발명은 상기 방법으로 제조된 표고버섯차를 제공한다.Next, the present invention provides shiitake mushroom tea prepared by the above method.

또한, 상기 표고버섯차는 DPPH 라디컬 소거활성이 증가된 것일 수 있다.Additionally, the shiitake mushroom tea may have increased DPPH radical scavenging activity.

또한, 상기 표고버섯차는 아질산염 소거능이 증가된 것일 수 있다.Additionally, the shiitake mushroom tea may have increased nitrite scavenging ability.

또한, 상기 표고버섯차는 총 폴리페놀 함량이 증가된 것일 수 있다.Additionally, the shiitake mushroom tea may have an increased total polyphenol content.

또한, 상기 표고버섯차는 베타글루칸 함량이 증가된 것일 수 있다.Additionally, the shiitake mushroom tea may have an increased beta-glucan content.

또한, 상기 표고버섯차는 아미노산 및 필수아미노산 함량이 증가된 것일 수 있다.Additionally, the shiitake mushroom tea may have increased amino acid and essential amino acid content.

이하, 본 발명의 실시예를 첨부된 도면을 참고하여 보다 상세하게 설명하도록 한다. 그러나, 하기의 실시예는 본 발명의 내용을 구체화하기 위한 것일 뿐, 이에 의해 본 발명이 한정되는 것은 아닐 것이다.Hereinafter, embodiments of the present invention will be described in more detail with reference to the attached drawings. However, the following examples are only intended to embody the content of the present invention, and the present invention is not limited thereto.

<제조예 1> 로스팅된 표고버섯 시료의 준비<Preparation Example 1> Preparation of roasted shiitake mushroom samples

표고버섯 시료는 a) 표고버섯의 생시료 l,500g을 칼날회전수 1,200 rpm/min으로 7 mm 크기로 2분간 세절하는 단계; b) 온수를 이용한 대류식 열풍건조기를 이용하여 1차 온도조건으로 건조 기내 45°C, 온수온도 55°C, 버섯 건조시간 30시간 유지하는 단계; c) 2차 온도조건으로 건조기내 60°C, 온수온도 70°C, 건조시간 12시간 건조하는 단계; d) 수분함량을 측정하여 수분율 7%이하로 건조하는 단계; e) 로스팅 조건으로 로스팅기기는 예열 75°C에서 건조된 버섯을 넣고 드럼을 회전시키는 단계;를 거쳐 준비하였다.The shiitake mushroom sample is a) chopping 1,500 g of raw shiitake mushroom sample into 7 mm pieces for 2 minutes at a blade rotation speed of 1,200 rpm/min; b) Using a convection hot air dryer using hot water, maintaining the primary temperature conditions of 45°C in the drying machine, 55°C hot water temperature, and 30 hours of mushroom drying time; c) Drying in the dryer at 60°C, hot water temperature 70°C, and drying time 12 hours as secondary temperature conditions; d) measuring the moisture content and drying it to a moisture content of 7% or less; e) For roasting conditions, the roasting device was prepared by preheating to 75°C, adding dried mushrooms and rotating the drum.

이어서, f) 1차 100°C에서 5분간 로스팅 처리하는 단계; g) 2차 130°C에서 5분간 로스팅처리 하는 단계; h) 3차 170°C에서 5분간 로스팅 처리하는 단계; I) 로스팅처리가 완료되는 즉시 냉각하여 상온까지 온도를 하강시키는 단계;를 통해 로스팅된 표고버섯 시료를 수득하였다.Subsequently, f) first roasting process at 100°C for 5 minutes; g) Second roasting at 130°C for 5 minutes; h) Third roasting step at 170°C for 5 minutes; A roasted shiitake mushroom sample was obtained through the step of: I) cooling immediately after the roasting process was completed and lowering the temperature to room temperature.

<제조예 2> 귀리 시료 및 현미 시료의 준비<Preparation Example 2> Preparation of oat samples and brown rice samples

귀리 시료 및 현미 시료는 귀리 및 현미 각각 1kg을 220℃에서 5분간 로스팅 처리하여 제조하였다.Oat samples and brown rice samples were prepared by roasting 1 kg each of oats and brown rice at 220°C for 5 minutes.

<실시예1 내지 6> 표고버섯 및 곡물류가 혼합된 표고버섯차의 제조 <Examples 1 to 6> Preparation of shiitake mushroom tea mixed with shiitake mushrooms and grains

본 발명자들은 곡물류 첨가 혼합비율별 영양성분 (베타글루칸, 아미노산, 필수아미노산) 함량 차이를 확인하기 위해, 하기 표 1의 조성으로 제조예 1의 로스팅된 표고버섯 시료 및 제조예 2의 귀리 또는 현미를 혼합하여 티백 (tea-bag)을 제조하고, 상기 티백 (tea-bag)을 70 ℃의 물에 10분 동안 침지하여 표고버섯차를 제조하였다.The present inventors used the roasted shiitake mushroom sample of Preparation Example 1 and the oats or brown rice of Preparation Example 2 with the composition shown in Table 1 below to confirm the difference in nutritional content (beta-glucan, amino acid, essential amino acid) according to the mixing ratio of grain addition. A tea-bag was prepared by mixing, and the tea-bag was immersed in water at 70° C. for 10 minutes to prepare shiitake mushroom tea.

표고버섯 (g)Shiitake mushrooms (g) 귀리 (g)Oats (g) 현미 (g)Brown rice (g) 실시예1Example 1 6060 6060 -- 실시예2Example 2 3030 9090 -- 실시예3Example 3 9090 3030 -- 실시예4Example 4 6060 -- 6060 실시예5Example 5 3030 -- 9090 실시예6Example 6 9090 -- 3030

<실시예 1-1 내지 6-4> 침지 조건별 표고버섯 및 곡물류가 혼합된 표고버섯차의 제조<Examples 1-1 to 6-4> Preparation of shiitake mushroom tea mixed with shiitake mushrooms and grains according to immersion conditions

이어서, 본 발명자들은 침지 조건별 표고버섯차의 생리활성 (항산화 활성 및 아질산염소거능) 및 폴리페놀 함량 차이를 확인하기 위해, 하기 표 2의 조성으로 제조예 1의 로스팅된 표고버섯 시료 및 제조예 2의 귀리 또는 현미를 혼합하여 티백 (tea-bag)을 제조하고, 상기 티백 (tea-bag)을 하기 표 2의 침지 조건으로 70℃ 또는 100℃의 물에 3분 또는 10분 동안 침지하여 표고버섯차를 제조하였다.Next, the present inventors used roasted shiitake mushroom samples of Preparation Example 1 and Preparation Example 2 with the compositions in Table 2 below to confirm differences in physiological activity (antioxidant activity and nitrite scavenging ability) and polyphenol content of shiitake mushroom tea according to immersion conditions. Prepare a tea bag by mixing oats or brown rice, and immerse the tea bag in water at 70°C or 100°C for 3 or 10 minutes under the immersion conditions shown in Table 2 below to produce shiitake mushrooms. Tea was manufactured.

표고버섯 (g)Shiitake mushrooms (g) 귀리 (g)Oats (g) 현미 (g)Brown rice (g) 온도 (℃)Temperature (℃) 시간 (분)time (minutes) 실시예1-1Example 1-1 6060 6060 -- 7070 1010 실시예1-2Example 1-2 6060 6060 -- 7070 33 실시예1-3Example 1-3 6060 6060 -- 100100 1010 실시예1-4Example 1-4 6060 6060 -- 100100 33 실시예2-1Example 2-1 3030 9090 -- 7070 1010 실시예2-2Example 2-2 3030 9090 -- 7070 33 실시예2-3Example 2-3 3030 9090 -- 100100 1010 실시예2-4Example 2-4 3030 9090 -- 100100 33 실시예3-1Example 3-1 9090 3030 -- 7070 1010 실시예3-2Example 3-2 9090 3030 -- 7070 33 실시예3-3Example 3-3 9090 3030 -- 100100 1010 실시예3-4Example 3-4 9090 3030 -- 100100 33 실시예4-1Example 4-1 6060 -- 6060 7070 1010 실시예4-2Example 4-2 6060 -- 6060 7070 33 실시예4-3Example 4-3 6060 -- 6060 100100 1010 실시예4-4Example 4-4 6060 -- 6060 100100 33 실시예5-1Example 5-1 3030 -- 9090 7070 1010 실시예5-2Example 5-2 3030 -- 9090 7070 33 실시예5-3Example 5-3 3030 -- 9090 100100 1010 실시예5-4Example 5-4 3030 -- 9090 100100 33 실시예6-1Example 6-1 9090 -- 3030 7070 1010 실시예6-2Example 6-2 9090 -- 3030 7070 33 실시예6-3Example 6-3 9090 -- 3030 100100 1010 실시예6-4Example 6-4 9090 -- 3030 100100 33

<실험예 1> 곡물 혼합비율별 표고버섯차의 베타글루칸 함량 확인<Experimental Example 1> Confirmation of beta-glucan content of shiitake mushroom tea according to grain mixing ratio

상기 실시예 1 내지 6의 곡물 혼합비율별 표고버섯차의 베타글루칸 함량을 확인하였다. 실시예 1 내지 3은 표고버섯 및 귀리를 1:1, 1:3 또는 3:1의 비율로 혼합한 것이며, 실시예 4 내지 6은 표고버섯 및 현미를 1:1, 1:3 또는 3:1의 비율로 혼합한 것이다.The beta-glucan content of the shiitake mushroom tea according to the grain mixing ratio of Examples 1 to 6 was confirmed. Examples 1 to 3 are shiitake mushrooms and oats mixed at a ratio of 1:1, 1:3 or 3:1, and Examples 4 to 6 are shiitake mushrooms and brown rice mixed at a ratio of 1:1, 1:3 or 3:1. It is mixed in a ratio of 1.

각 표고버섯차의 베타글루칸 함량 확인 결과는 표 3에 나타내었다.The results of confirming the beta-glucan content of each shiitake mushroom tea are shown in Table 3.

표 3에 나타낸 바와 같이, 표고버섯 및 현미가 혼합된 표고버섯차 중 표고버섯 및 현미를 3:1로 혼합하여 제조된 실시예 6의 베타글루칸 함량은 34.36 % (w/w)로 다른 실시예들과 비교하여도 베타글루칸 함량이 현저히 높은 것을 확인하였다.As shown in Table 3, among the shiitake mushroom tea mixed with shiitake mushrooms and brown rice, the beta-glucan content of Example 6, prepared by mixing shiitake mushrooms and brown rice at a ratio of 3:1, was 34.36% (w/w), compared to other examples. It was confirmed that the beta-glucan content was significantly higher compared to the others.

특히, 1:1 및 1:3의 혼합비율에서는 표고버섯 및 귀리가 혼합된 표고버섯차(실시예 1 및 2)가 동일한 혼합비율의 표고버섯 및 현미가 혼합된 표고버섯차(실시예 4 및 5)와 비교하여 더 높은 베타글루칸 함량을 보인 반면에, 3:1의 혼합비율에서는 표고버섯 및 현미가 혼합된 표고버섯차(실시예 6)가 더 많은 베타글루칸을 포함하고 있었다.In particular, at mixing ratios of 1:1 and 1:3, shiitake mushroom tea mixed with shiitake mushrooms and oats (Examples 1 and 2) was compared to shiitake mushroom tea mixed with shiitake mushrooms and brown rice at the same mixing ratio (Examples 4 and 2). While it showed a higher beta-glucan content compared to 5), at a mixing ratio of 3:1, shiitake mushroom tea mixed with shiitake mushrooms and brown rice (Example 6) contained more beta-glucan.

혼합비율
(표고+귀리)
mixing ratio
(shiitake + oats)
혼합비율
(표고+현미)
mixing ratio
(Shiitake+brown rice)
베타글루칸 함량
(% w/w)
Beta-glucan content
(%w/w)
실시예1Example 1 1:11:1 27.11 ± 0.1727.11 ± 0.17 실시예2Example 2 1:31:3 18.09 ± 0.6918.09 ± 0.69 실시예3Example 3 3:13:1 29.22 ± 0.1029.22 ± 0.10 실시예4Example 4 1:11:1 20.83 ± 0.3220.83 ± 0.32 실시예5Example 5 1:31:3 15.68 ± 0.3215.68 ± 0.32 실시예6Example 6 3:13:1 34.36 ± 0.0734.36 ± 0.07

<실험예 2> 곡물 혼합비율별 표고버섯차의 아미노산 함량 확인<Experimental Example 2> Confirmation of amino acid content of shiitake mushroom tea according to grain mixing ratio

상기 실시예 1 내지 6의 곡물 혼합비율별 표고버섯차의 아미노산 및 필수아미노산 함량을 확인하였다. 각 표고버섯차의 총 아미노산 및 필수아미노산 함량 확인 과는 표 4에 나타내었다.The amino acid and essential amino acid contents of shiitake mushroom tea were confirmed for each grain mixing ratio in Examples 1 to 6. The total and essential amino acid contents of each shiitake mushroom tea are shown in Table 4.

표 4에 나타낸 바와 같이, 표고버섯 및 현미가 혼합된 표고버섯차 중 표고버섯 및 현미를 3:1로 혼합하여 제조된 실시예 6의 총 아미노산 함량은 1303.6 mg/kg, 필수아미노산 함량은 440.6 mg/kg으로 다른 실시예들과 비교하여도 총 아미노산 및 필수아미노산 함량이 현저히 높은 것을 확인하였다.As shown in Table 4, among the shiitake mushroom teas mixed with shiitake mushrooms and brown rice, the total amino acid content of Example 6 prepared by mixing shiitake mushrooms and brown rice in a ratio of 3:1 was 1303.6 mg/kg, and the essential amino acid content was 440.6 mg. It was confirmed that the total amino acid and essential amino acid content was significantly high compared to other examples at /kg.

또한, 표고버섯 및 현미가 혼합된 표고버섯차 중 표고버섯 및 현미를 1:1 및 3:1의 비율로 혼합하여 제조된 실시예 4 및 6의 표고버섯차의 경우, 표고버섯 및 현미를 1:3의 비율로 혼합하여 제조된 실시예 5의 표고버섯차보다 총 아미노산 및 필수아미노산 함량이 높은 것을 확인하였다. 이러한 결과는 표고버섯보다 현미의 비율이 높아질 경우, 표고버섯차 내 존재하는 총 아미노산 및 필수아미노산의 함량이 적어진다는 것을 의미한다.In addition, in the case of the shiitake mushroom tea of Examples 4 and 6, which was prepared by mixing shiitake mushrooms and brown rice in a ratio of 1:1 and 3:1, the shiitake mushrooms and brown rice were mixed at 1:1. It was confirmed that the total amino acid and essential amino acid content was higher than the shiitake mushroom tea of Example 5 prepared by mixing at a ratio of :3. These results mean that when the ratio of brown rice is higher than that of shiitake mushrooms, the content of total amino acids and essential amino acids present in shiitake mushroom tea decreases.

혼합비율
(표고+귀리)
mixing ratio
(shiitake + oats)
혼합비율
(표고+현미)
mixing ratio
(Shiitake+brown rice)
아미노산 함량 (mg/kg)Amino acid content (mg/kg)
총 아미노산total amino acids 필수아미노산essential amino acids 실시예1Example 1 1:11:1 1093.3 ± 29.11093.3 ± 29.1 374.2 ± 19.7374.2 ± 19.7 실시예2Example 2 1:31:3 1012.2 ± 43.91012.2 ± 43.9 335.6 ± 26.0335.6 ± 26.0 실시예3Example 3 3:13:1 1230.3 ± 40.51230.3 ± 40.5 397.6 ± 24.3397.6 ± 24.3 실시예4Example 4 1:11:1 1177.5 ± 53.81177.5 ± 53.8 380.3 ± 25.9380.3 ± 25.9 실시예5Example 5 1:31:3 951.6 ± 42.7951.6 ± 42.7 326.8 ± 24.8326.8 ± 24.8 실시예6Example 6 3:13:1 1303.6 ± 64.01303.6 ± 64.0 440.6 ± 10.2440.6 ± 10.2

<실험예 3> 침지 조건별 표고버섯차의 총 폴리페놀 함량 확인<Experimental Example 3> Confirmation of total polyphenol content of shiitake mushroom tea according to immersion conditions

상기 실시예 1-1 내지 6-4의 표고버섯차의 곡물 혼합비율 및 침지 조건별 총 폴리페놀 함량을 확인하였다. 각 표고버섯차의 곡물 혼합비율 및 침지 조건별 총 폴리페놀 함량 확인 결과는 표 5에 나타내었다.The total polyphenol content of the shiitake mushroom tea of Examples 1-1 to 6-4 was confirmed by grain mixing ratio and immersion conditions. The results of confirming the total polyphenol content by grain mixing ratio and immersion conditions of each shiitake mushroom tea are shown in Table 5.

표 5에 나타낸 바와 같이, 실시예 4-1 내지 6-4의 표고버섯차 (표 4 오른쪽)는 실시예 1-1 내지 3-4의 표고버섯차 (표 4 왼쪽)와 비교하여 더 많은 폴리페놀을 포함하고 있었다.As shown in Table 5, the shiitake mushroom tea of Examples 4-1 to 6-4 (right side of Table 4) has more poly than the shiitake mushroom tea of Examples 1-1 to 3-4 (left side of Table 4). It contained phenol.

특히, 표고버섯 및 현미를 1:1의 비율로 혼합하고 70℃의 물에 10분 동안 침지하여 제조된 실시예 4-1의 표고버섯차의 경우, 표고버섯 및 귀리가 혼합된 실시예 1-1 내지 3-4의 표고버섯차 뿐만 아니라 다른 실시예들과 비교하여도 표고버섯차에 포함된 총 폴리페놀 함량이 현저히 높은 것을 확인하였다.In particular, in the case of the shiitake mushroom tea of Example 4-1, which was prepared by mixing shiitake mushrooms and brown rice at a ratio of 1:1 and immersing it in water at 70° C. for 10 minutes, Example 1- where shiitake mushrooms and oats were mixed It was confirmed that the total polyphenol content contained in the shiitake mushroom tea was significantly high when compared to the shiitake mushroom teas of 1 to 3-4 as well as other examples.

표고+귀리shiitake + oats 총 폴리페놀 함량
(mg GAE/g)
Total polyphenol content
(mg GAE/g)
표고+현미Shiitake + brown rice 총 폴리페놀 함량
(mg GAE/g)
Total polyphenol content
(mg GAE/g)
실시예1-1Example 1-1 6.70 ± 0.146.70 ± 0.14 실시예4-1Example 4-1 8.84 ± 0.368.84 ± 0.36 실시예1-2Example 1-2 6.86 ± 0.246.86 ± 0.24 실시예4-2Example 4-2 7.57 ± 0.277.57 ± 0.27 실시예1-3Example 1-3 6.22 ± 0.146.22 ± 0.14 실시예4-3Example 4-3 6.78 ± 0.146.78 ± 0.14 실시예1-4Example 1-4 6.70 ± 0.276.70 ± 0.27 실시예4-4Example 4-4 7.25 ± 0.367.25 ± 0.36 실시예2-1Example 2-1 7.17 ± 0.147.17 ± 0.14 실시예5-1Example 5-1 7.97 ± 0.147.97 ± 0.14 실시예2-2Example 2-2 6.70 ± 0.276.70 ± 0.27 실시예5-2Example 5-2 7.41 ± 0.147.41 ± 0.14 실시예2-3Example 2-3 6.22 ± 0.606.22 ± 0.60 실시예5-3Example 5-3 6.22 ± 0.146.22 ± 0.14 실시예2-4Example 2-4 6.38 ± 0.636.38 ± 0.63 실시예5-4Example 5-4 7.49 ± 0.147.49 ± 0.14 실시예3-1Example 3-1 6.94 ± 0.146.94 ± 0.14 실시예6-1Example 6-1 6.14 ± 0.246.14 ± 0.24 실시예3-2Example 3-2 7.25 ± 0.147.25 ± 0.14 실시예6-2Example 6-2 5.98 ± 0.145.98 ± 0.14 실시예3-3Example 3-3 5.51 ± 0.275.51 ± 0.27 실시예6-3Example 6-3 7.73 ± 0.147.73 ± 0.14 실시예3-4Example 3-4 5.27 ± 0.275.27 ± 0.27 실시예6-4Example 6-4 6.30 ± 0.146.30 ± 0.14

<실험예 4> 침지 조건별 표고버섯차의 아질산염 소거능 확인<Experimental Example 4> Confirmation of nitrite scavenging ability of shiitake mushroom tea according to immersion conditions

상기 실시예 1-1 내지 6-4의 표고버섯차의 곡물 혼합비율 및 침지 조건별 아질산염 소거능을 확인하였다. 각 표고버섯차의 곡물 혼합비율 및 침지 조건별 아질산염 소거능 확인 결과는 도 1에 나타내었다.The nitrite scavenging ability of the shiitake mushroom tea of Examples 1-1 to 6-4 was confirmed depending on the grain mixing ratio and immersion conditions. The results of confirming the nitrite scavenging ability of each shiitake mushroom tea by grain mixing ratio and immersion conditions are shown in Figure 1.

도 1은 곡물첨가 표고버섯차의 추출온도 및 시간별 아질산염 소거능 확인 결과를 나타낸 그래프이다. 도 1A는 70℃, 도 1B는 100℃에서 침지한 시간 및 혼합비율별 아질산염 소거능 확인 결과이다. LE는 표고버섯, O는 귀리, B는 현미를 의미한다.Figure 1 is a graph showing the results of confirming the nitrite scavenging ability of grain-added shiitake mushroom tea by extraction temperature and time. Figure 1A shows the results of confirming nitrite scavenging ability by immersion time and mixing ratio at 70°C and Figure 1B at 100°C. LE stands for shiitake mushrooms, O stands for oats, and B stands for brown rice.

도 1에 나타낸 바와 같이, 실시예 1-1 내지 3-4의 표고버섯차 (표고+귀리, 도 1의 LE+O)는 실시예 4-1 내지 6-4의 표고버섯차 (표고+현미, 도 1의 LE+B)와 비교하여 현저히 우수한 아질산염 소거능을 가지는 것을 확인하였다.As shown in Figure 1, the shiitake mushroom tea (shiitake + oats, LE + O in Figure 1) of Examples 1-1 to 3-4 is the shiitake mushroom tea (shiitake + brown rice) of Examples 4-1 to 6-4. , it was confirmed that it had a significantly superior nitrite scavenging ability compared to LE+B in Figure 1).

특히, 표고버섯 및 귀리가 혼합된 표고버섯차 중 표고버섯 및 귀리를 1:1의 비율로 혼합하고 100℃의 물에 3분 동안 침지하여 제조된 실시예 1-4의 표고버섯차의 경우, 표고버섯 및 현미가 혼합된 실시예 4-1 내지 6-4의 표고버섯차 뿐만 아니라 다른 실시예들과 비교하여도 현저히 우수한 아질산염 소거능을 가지는 것을 확인하였다.In particular, in the case of the shiitake mushroom tea of Example 1-4, which was prepared by mixing shiitake mushrooms and oats in a ratio of 1:1 and steeping in water at 100°C for 3 minutes, It was confirmed that the shiitake mushroom tea of Examples 4-1 to 6-4, which was a mixture of shiitake mushrooms and brown rice, had a significantly superior nitrite scavenging ability compared to other examples as well as the shiitake mushroom tea of Examples 4-1 to 6-4.

<실험예 5> 침지 조건별 표고버섯차의 항산화 활성 확인<Experimental Example 5> Confirmation of antioxidant activity of shiitake mushroom tea according to immersion conditions

상기 실시예 1-1 내지 6-4의 표고버섯차의 곡물 혼합비율 및 침지 조건별 항산화 활성을 확인하기 위해, DPPH 라디컬 소거능을 확인하였다. 각 표고버섯차의 곡물 혼합비율 및 침지 조건별 DPPH 라디컬 소거능 확인 결과는 도 2에 나타내었다.In order to confirm the antioxidant activity of the shiitake mushroom tea of Examples 1-1 to 6-4 according to the grain mixing ratio and immersion conditions, the DPPH radical scavenging activity was confirmed. The results of confirming the DPPH radical scavenging ability of each shiitake mushroom tea by grain mixing ratio and immersion conditions are shown in Figure 2.

도 2는 곡물첨가 표고버섯차의 추출온도 및 시간별 DPPH 라디컬 소거능 확인 결과를 나타낸 그래프이다. 도 2A는 70℃, 도 2B는 100℃에서 침지한 시간 및 혼합비율별 DPPH 라디컬 소거능 확인 결과이다. LE는 표고버섯, O는 귀리, B는 현미를 의미한다.Figure 2 is a graph showing the results of confirming the DPPH radical scavenging ability of grain-added shiitake mushroom tea by extraction temperature and time. Figure 2A shows the results of confirming the DPPH radical scavenging ability by immersion time and mixing ratio at 70°C and Figure 2B at 100°C. LE stands for shiitake mushrooms, O stands for oats, and B stands for brown rice.

도 2에 나타낸 바와 같이, 표고버섯 및 귀리가 혼합된 표고버섯차 중 표고버섯 및 귀리를 1:1의 비율로 혼합하고 100℃의 물에 3분 동안 침지하여 제조된 실시예 1-4의 표고버섯차의 경우, 표고버섯 및 현미가 혼합된 실시예 4-1 내지 6-4의 표고버섯차 뿐만 아니라 다른 실시예들과 비교하여도 현저히 우수한 DPPH 라디컬 소거능을 가지는 것을 확인하였다.As shown in Figure 2, the shiitake tea of Example 1-4 was prepared by mixing shiitake mushrooms and oats in a ratio of 1:1 and immersing it in water at 100°C for 3 minutes. In the case of mushroom tea, it was confirmed that it had a significantly superior DPPH radical scavenging ability compared to other examples as well as the shiitake mushroom tea of Examples 4-1 to 6-4, which was a mixture of shiitake mushrooms and brown rice.

상기 실험예 1 내지 5의 결과를 종합해보면, 표고버섯 및 현미를 혼합하여 제조된 표고버섯차의 경우, 현저히 높은 영양성분 (폴리페놀, 베타글루칸 및 아미노산)을 포함하고, 표고버섯 및 귀리를 혼합하여 제조된 표고버섯차의 경우, 현저히 우수한 항산화 활성 및 아질산염 소거능을 가진다는 것을 의미한다.Summarizing the results of Experimental Examples 1 to 5, it can be seen that shiitake mushroom tea prepared by mixing shiitake mushrooms and brown rice contains significantly higher nutrients (polyphenols, beta-glucans, and amino acids), and that shiitake mushrooms and oats are mixed. This means that shiitake mushroom tea produced in this way has significantly excellent antioxidant activity and nitrite scavenging ability.

이상에서 살펴본 바와 같이, 본 발명의 구체적인 실시예를 상세하게 설명되었으나, 본 발명의 사상을 이해하는 당업자는 동일한 사상의 범위 내에서 다른 구성요소를 추가, 변경, 삭제 등을 통하여, 퇴보적인 다른 발명이나 본 발명 사상의 범위 내에 포함되는 다른 실시예를 용이하게 제안할 수 있을 것이다. 그러므로 이상에서 기술한 실시예들은 모든 면에서 예시적인 것이며 한정적이 아닌 것으로 이해해야만 한다. 본 발명의 범위는 상술한 상세한 설명보다는 후술하는 특허청구의 범위에 의하여 나타내어지며, 특허청구의 범위의 의미 및 범위 그리고 그 균등 개념으로부터 도출되는 모든 변경 또는 변형된 형태가 본 발명의 범위에 포함되는 것으로 해석되어야 한다.As discussed above, specific embodiments of the present invention have been described in detail, but those skilled in the art who understand the spirit of the present invention may add, change, delete, etc. other components within the scope of the same spirit, thereby creating other regressive inventions. However, other embodiments that are included within the scope of the present invention can be easily proposed. Therefore, the embodiments described above should be understood in all respects as illustrative and not restrictive. The scope of the present invention is indicated by the scope of the claims described below rather than the detailed description above, and all changes or modified forms derived from the meaning and scope of the claims and their equivalent concepts are included in the scope of the present invention. It should be interpreted as

Claims (8)

로스팅 기기에 건조된 표고버섯을 넣고 90 내지 110℃에서 4 내지 6분간 1차로 로스팅 처리하는 1단계;
120 내지 140℃에서 4 내지 6분간 2차로 로스팅 처리하는 2단계;
160 내지 180℃에서 4 내지 6분간 3차로 로스팅 처리하는 3단계;
현미 또는 귀리를 210 내지 220℃에서 4 내지 6분간 로스팅 처리하는 4단계; 및
상기 로스팅 처리된 표고버섯과 로스팅 처리된 현미 또는 귀리를 혼합하여 티백 (Tea bag)으로 제조하는 5단계;를 포함하는, 생리활성과 영양성분이 증진된 표고버섯차 제조방법에서,
상기 생리활성은 항산화 활성 및 아질산염 소거능이고,
상기 영양성분은 폴리페놀, 베타글루칸 및 아미노산인, 제조방법.
Step 1: Put dried shiitake mushrooms in a roasting machine and roast them at 90 to 110°C for 4 to 6 minutes;
Step 2 of secondary roasting at 120 to 140°C for 4 to 6 minutes;
Step 3 of third roasting at 160 to 180°C for 4 to 6 minutes;
Step 4, roasting brown rice or oats at 210 to 220°C for 4 to 6 minutes; and
In the method for producing shiitake mushroom tea with improved physiological activity and nutritional components, including the 5th step of mixing the roasted shiitake mushrooms and the roasted brown rice or oats to produce a tea bag,
The physiological activities are antioxidant activity and nitrite scavenging activity,
The manufacturing method wherein the nutrients are polyphenol, beta-glucan, and amino acid.
제1항에 있어서,
상기 1단계의 건조된 표고버섯은
a) 표고버섯의 생시료를 6 내지 8mm 크기로 세절하는 단계;
b) 온수를 이용한 대류식 열풍건조기를 이용하여 1차 온도조건으로 건조기내 40 내지 50℃, 온수온도 50 내지 60℃의 조건으로 버섯을 25 내지 35시간 동안 건조하는 단계;
c) 2차 온도조건으로 건조기내 55 내지 65℃, 온수온도 65 내지 75℃의 조건으로 버섯을 11 내지 13시간 동안 건조하는 단계; 및
d) 상기 c)단계 후에 수분함량을 측정하여 수분율 1 내지 7%까지 추가적으로 건조시켜 건조된 표고버섯을 제조하는 단계;를 거쳐 제조된 것을 특징으로 하는, 생리활성과 영양성분이 증진된 표고버섯차 제조방법.
According to paragraph 1,
The dried shiitake mushrooms in step 1 above are
a) Chopping raw samples of shiitake mushrooms into pieces of 6 to 8 mm in size;
b) using a convection hot air dryer using hot water to dry mushrooms for 25 to 35 hours under the primary temperature conditions of 40 to 50°C in the dryer and 50 to 60°C in hot water;
c) drying the mushrooms for 11 to 13 hours in a dryer at a secondary temperature of 55 to 65°C and a hot water temperature of 65 to 75°C; and
d) producing dried shiitake mushrooms by measuring the moisture content after step c) and additionally drying the moisture content to 1 to 7%; Shiitake mushroom tea with enhanced physiological activity and nutritional components Manufacturing method.
제1항 또는 제2항의 방법으로 제조된 표고버섯차.
Shiitake mushroom tea manufactured by the method of paragraph 1 or 2.
제3항에 있어서, 상기 표고버섯차는 DPPH 라디컬 소거활성이 증가된 것을 특징으로 하는, 표고버섯차.
The shiitake mushroom tea according to claim 3, wherein the shiitake mushroom tea has increased DPPH radical scavenging activity.
제3항에 있어서, 상기 표고버섯차는 아질산염 소거능이 증가된 것을 특징으로 하는, 표고버섯차.
The shiitake mushroom tea according to claim 3, wherein the shiitake mushroom tea has an increased nitrite scavenging ability.
제3항에 있어서, 상기 표고버섯차는 총 폴리페놀 함량이 증가된 것을 특징으로 하는, 표고버섯차.
The shiitake mushroom tea according to claim 3, wherein the shiitake mushroom tea has an increased total polyphenol content.
제3항에 있어서, 상기 표고버섯차는 베타글루칸 함량이 증가된 것을 특징으로 하는, 표고버섯차.
The shiitake mushroom tea according to claim 3, wherein the shiitake mushroom tea has an increased beta-glucan content.
제3항에 있어서, 상기 표고버섯차는 아미노산 및 필수아미노산 함량이 증가된 것을 특징으로 하는, 표고버섯차.The shiitake mushroom tea according to claim 3, wherein the shiitake mushroom tea has an increased content of amino acids and essential amino acids.
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