KR102580636B1 - manufacturing method of vegetarian hamburger patty used natural colorant - Google Patents

manufacturing method of vegetarian hamburger patty used natural colorant Download PDF

Info

Publication number
KR102580636B1
KR102580636B1 KR1020200182081A KR20200182081A KR102580636B1 KR 102580636 B1 KR102580636 B1 KR 102580636B1 KR 1020200182081 A KR1020200182081 A KR 1020200182081A KR 20200182081 A KR20200182081 A KR 20200182081A KR 102580636 B1 KR102580636 B1 KR 102580636B1
Authority
KR
South Korea
Prior art keywords
vegetable
weight
parts
comparative example
hamburger patty
Prior art date
Application number
KR1020200182081A
Other languages
Korean (ko)
Other versions
KR20220091659A (en
Inventor
장혜원
최윤상
전기홍
홍정선
유민희
Original Assignee
한국식품연구원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 한국식품연구원 filed Critical 한국식품연구원
Priority to KR1020200182081A priority Critical patent/KR102580636B1/en
Publication of KR20220091659A publication Critical patent/KR20220091659A/en
Application granted granted Critical
Publication of KR102580636B1 publication Critical patent/KR102580636B1/en

Links

Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00Working-up of proteins for foodstuffs
    • A23J3/22Working-up of proteins for foodstuffs by texturising
    • A23J3/225Texturised simulated foods with high protein content
    • A23J3/227Meat-like textured foods
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23DEDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
    • A23D9/00Other edible oils or fats, e.g. shortenings, cooking oils
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/006Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from vegetable materials
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/14Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00Working-up of proteins for foodstuffs
    • A23J3/14Vegetable proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/40Meat products; Meat meal; Preparation or treatment thereof containing additives
    • A23L13/42Additives other than enzymes or microorganisms in meat products or meat meals
    • A23L13/426Addition of proteins, carbohydrates or fibrous material from vegetable origin other than sugars or sugar alcohols
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/10Natural spices, flavouring agents or condiments; Extracts thereof
    • A23L27/105Natural spices, flavouring agents or condiments; Extracts thereof obtained from liliaceae, e.g. onions, garlic
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23PSHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00Shaping or working of foodstuffs characterised by the products
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00Function of food ingredients
    • A23V2200/04Colour
    • A23V2200/044Colouring
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
    • A23V2250/50Polysaccharides, gums
    • A23V2250/502Gums
    • A23V2250/5026Alginate
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
    • A23V2250/50Polysaccharides, gums
    • A23V2250/502Gums
    • A23V2250/5036Carrageenan
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
    • A23V2250/76Yeasts
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00Processes
    • A23V2300/24Heat, thermal treatment

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Nutrition Science (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Seeds, Soups, And Other Foods (AREA)
  • Meat, Egg Or Seafood Products (AREA)

Abstract

본 발명은 식물성 햄버거 패티의 제조방법에 관한 것으로, 홍국균 색소와 비트색소 또는 락색소를 이용한 식물성 햄버거 패티는, 고온으로 조리시에도 남녀노소 누구나 거부감없이 애용할 수 있도록 특유의 육류의 식감과 색감을 제공할 수 있다는 장점이 있다.The present invention relates to a method of manufacturing a vegetable hamburger patty, and the vegetable hamburger patty using Hong Guk-Gyun coloring and beet coloring or rock coloring has a unique meat texture and color that can be used by anyone regardless of age or gender even when cooked at high temperature. There is an advantage in being able to provide it.

Description

식물성 햄버거 패티의 제조방법{manufacturing method of vegetarian hamburger patty used natural colorant}Manufacturing method of vegetarian hamburger patty {manufacturing method of vegetarian hamburger patty used natural colorant}

본 발명은 식물성 햄버거 패티의 제조방법에 관한 것으로, 원래의 햄버거 패티가 갖는 독특한 향과 색이 살아있는 식물성 햄버거 패티의 제조방법에 관한 것이다.The present invention relates to a method of manufacturing a plant-based hamburger patty, and to a method of manufacturing a plant-based hamburger patty that retains the unique flavor and color of the original hamburger patty.

최근, 서구문명의 현대인들은 육류 음식이 과체중, 비만 등 성인병을 유발하는 요인으로 인식됨에 따라 가급적 육류 음식을 피하고 다이어트 식품으로서 식물성 위주의 음식을 먹으려고 하고 있으나 대부분의 사람들은 육류의 음식에 익숙해져 있어 육류에 대한 유혹을 버릴 수 없는 것이 현실이다. 이에 따라 육류의 식감을 살리면서도 다이어트 식품으로 각광받는 육류 대체식품에 대한 관심이 높아지고 있다. 따라서, 육류 대체식품을 제조하기 위한 새로운 식품소재의 개발 및 생산 기술개발이 요구되고 있는 실정이다.Recently, modern people in Western civilization are trying to avoid meat foods as much as possible and eat plant-based foods as diet foods, as meat foods are recognized as a factor that causes adult diseases such as overweight and obesity. However, most people are accustomed to meat foods. The reality is that we cannot give up the temptation to eat meat. Accordingly, interest in meat substitutes that preserve the texture of meat and are popular as diet foods is increasing. Therefore, there is a need for the development of new food materials and production technology for producing meat substitutes.

이러한 육류 대체식품은 대두 단백을 중심으로 개발되어 왔다. 그러나 이러한 종래 기술들에서 사용한 대두 단백은 외형적으로 육류의 형태를 구현하였지만, 조리 후에도 육류 특유의 색감과 형태를 지니는 데에는 한계가 있어 저민 고기 또는 다진 고기 또는 소시지 등의 대용으로만 주로 사용되어 왔고, 대두의 이취가 여전히 잔존하는 단점이 있다. 이를 해결하기 위하여 조리 후에도 육류의 식감과 색감 및 형태를 지닐 수 있는 식물성 패티의 개발이 요구되고 있는 실정이다.These meat substitutes have been developed focusing on soy protein. However, the soy protein used in these prior technologies has the appearance of meat, but has limitations in retaining the unique color and shape of meat even after cooking, so it has been mainly used as a substitute for minced meat, minced meat, or sausage. , there is a disadvantage that the off-flavor of soybeans still remains. To solve this problem, there is a need for the development of vegetable patties that can retain the texture, color, and shape of meat even after cooking.

특허문헌 1. 대한민국 등록특허 제10-2027091호Patent Document 1. Republic of Korea Patent No. 10-2027091

본 발명이 해결하고자 하는 과제는 조리 후에도 육류의 식감과 색감 및 형태를 갖는 식물성 햄버거 패티 제조방법을 제공하는 것이다.The problem to be solved by the present invention is to provide a method for manufacturing plant-based hamburger patties that have the texture, color, and shape of meat even after cooking.

본 발명이 해결하고자 하는 다른 과제는 상기 제조방법으로 제조된 식물성 햄버거 패티를 제공하는 것이다.Another problem to be solved by the present invention is to provide a vegetable hamburger patty manufactured by the above manufacturing method.

본 발명은 상기 과제를 해결하기 위하여, a) 식물성 조직 단백질(TVP), 홍국균 분말 및 증류수를 혼합하여 식물성 조직 단백질을 수화시키는 단계; b) 상기 a) 단계에서 제조된 수화된 식물성 조직 단백질; 비트 색소, 락색소 또는 이들의 혼합물; 및 식물성 오일; 및 c) 상기 b) 단계의 성형한 식물성 햄버거 패티 조성물을 열처리하여 식물성 햄버거 패티를 제조하는 단계;를 포함하는 식물성 햄버거 패티 제조방법을 제공한다.In order to solve the above problem, the present invention includes the steps of: a) mixing vegetable tissue protein (TVP), red yeast rice powder, and distilled water to hydrate the vegetable tissue protein; b) hydrated vegetable tissue protein prepared in step a) above; Beet pigment, lac pigment, or mixtures thereof; and vegetable oil; and c) heat-treating the vegetable hamburger patty composition formed in step b) to produce a vegetable hamburger patty.

상기 a) 단계에서, 식물성 조직 단백질(TVP) 전체 100 중랑부를 기준으로, 상기 홍국균 분말이 0.01 내지 0.03 중량부 혼합되는 것일 수 있다.In step a), 0.01 to 0.03 parts by weight of the red yeast rice powder may be mixed, based on 100 total parts of vegetable tissue protein (TVP).

상기 b) 단계에서, 식물성 조직 단백질(TVP) 전체 100 중랑부를 기준으로, 상기 비트 색소 또는 락색소 0.2 내지 0.6 중량부, 식물성 오일 5 내지 15 중량부 혼합되는 것일 수 있다.In step b), based on a total of 100 parts of vegetable tissue protein (TVP), 0.2 to 0.6 parts by weight of the beet coloring or rock coloring and 5 to 15 parts by weight of vegetable oil may be mixed.

상기 식물성 오일은 대두유, 카놀라 오일, 코코넛 오일, 올리브 오일 및 팜 오일로 이루어진 군으로부터 선택되는 어느 하나 이상일 수 있다.The vegetable oil may be any one or more selected from the group consisting of soybean oil, canola oil, coconut oil, olive oil, and palm oil.

상기 b) 단계에. 상기 식물성 조직 단백질(TVP) 전체 100 중량부를 기준으로, 카라기난 0.5 내지 5 중량부, 알긴산 0.5 내지 5 중량부, 분리대두단백 0.5 내지 5 중량부, 밀식이섬유 0.5 내지 5 중량부, 소금 1 내지 2 중량부, 인산염 0.1 내지 1 중량부, MSG 0.1 내지 0.5 중량부 및 후추 0.001 내지 0.005 중량부를 더 첨가하는 것일 수 있다.In step b) above. Based on 100 parts by weight of the total vegetable tissue protein (TVP), 0.5 to 5 parts by weight of carrageenan, 0.5 to 5 parts by weight of alginic acid, 0.5 to 5 parts by weight of isolated soy protein, 0.5 to 5 parts by weight of wheat dietary fiber, and 1 to 2 parts by weight of salt. Parts by weight, 0.1 to 1 part by weight of phosphate, 0.1 to 0.5 parts by weight of MSG, and 0.001 to 0.005 parts by weight of pepper may be further added.

상기 c) 단계는 100 ℃에서 5 내지 10분간 가열한 것일 수 있다.Step c) may be performed by heating at 100°C for 5 to 10 minutes.

상기 c) 단계 후에, d) 상기 식물성 햄버거 패티의 겉면에 카라멜라이징된 양파를 바르는 단계;를 더 포함하는 것일 수 있다.After step c), d) applying caramelized onions to the outer surface of the vegetable hamburger patty may be further included.

본 발명은 상기 다른 과제를 해결하기 위하여, 상기 제조방법에 따라 제조된 식물성 햄버거 패티를 제공한다.In order to solve the above other problems, the present invention provides a vegetable hamburger patty manufactured according to the above manufacturing method.

본 발명에 따라 제조된, 홍국균 색소와 비트색소 또는 락색소를 이용한 식물성 햄버거 패티는, 고온으로 조리시에도 남녀노소 누구나 거부감없이 애용할 수 있도록 특유의 육류의 식감과 색감을 제공할 수 있다는 장점이 있다.The vegetable hamburger patty using red yeast coloring and beet coloring or rock coloring manufactured according to the present invention has the advantage of providing a unique meat texture and color so that anyone, regardless of age or gender, can use it without resistance even when cooked at high temperature. there is.

또한, 채식을 선호하는 사람들에게 유해성 논란이 계속되고 있는 색소를 사용하지 않은 기존 식물성 고기의 풍미에 뒤떨어지지 않으며 건강에 유익한 식물성 햄버거 패티를 제공할 수 있다는 장점도 있다.In addition, it has the advantage of providing a healthy plant-based hamburger patty that is comparable to the flavor of existing plant-based meat without coloring, which is still controversial for its harmfulness, to those who prefer vegetarianism.

도 1은 본 발명에 따른 식물성 햄버거 패티를 제조하는 각 단계를 촬영한 사진이다.
도 2는 퍼플그레이프색소, 비트색소, 적양배추색소, 액상적양배추, 포도과피추출색소, 락색소, 홍국색소, 적색 40호, 아스타잔틴색소 및 홍국적색소를 30 ℃에서 1 시간동안 방치한 후, 촬영한 도면이다.
도 3은 퍼플그레이프색소, 비트색소, 적양배추색소, 액상적양배추, 포도과피추출색소, 락색소, 홍국색소, 적색 40호, 아스타잔틴색소 및 홍국적색소를 60 ℃에서 1 시간동안 방치한 후, 촬영한 도면이다.
도 4는 퍼플그레이프색소, 비트색소, 적양배추색소, 액상적양배추, 포도과피추출색소, 락색소, 홍국색소, 적색 40호, 아스타잔틴색소 및 홍국적색소를 100 ℃에서 1 시간동안 방치한 후, 촬영한 도면이다.
도 5는 실시예 2 내지 6, 비교예 1 내지 12 및 비교예 22 내지 23에 따라 식물성 햄버거 패티를 제조하고, 이를 가열한 모습을 촬영한 사진이다.
도 6은 실시예 1 내지 실시예 2, 비교예 7, 비교예 8, 비교예 13, 비교예 16, 비교예 17, 비교예 18, 비교예 19, 비교예 20, 비교예 21에 따라 제조된 식물성 햄버거 패티의 사진이다.
도 7은 색소 첨가방법에 따른 식물성 햄버거 패티(실시예 7, 비교예 24 내지 27)의 제조과정을 촬영한 사진이다.
도 8은 가열하기 전, 실시예 7 및 비교예 24 내지 27의 식물성 햄버거 패티를 촬영한 사진이다.
Figure 1 is a photograph of each step of manufacturing a vegetable hamburger patty according to the present invention.
Figure 2 shows purple grape coloring, beet coloring, red cabbage coloring, liquid red cabbage, grape skin extract coloring, lacquering coloring, red yeast coloring, red No. 40, astaxanthin coloring and red yeast coloring being left at 30°C for 1 hour. This is a drawing taken afterward.
Figure 3 shows purple grape coloring, beet coloring, red cabbage coloring, liquid red cabbage, grape skin extract coloring, lacquering coloring, red yeast coloring, red No. 40, astaxanthin coloring and red yeast coloring being left at 60°C for 1 hour. This is a drawing taken afterward.
Figure 4 shows purple grape color, beet color, red cabbage color, liquid red cabbage, grape skin extract color, rock color, red yeast color, red No. 40, astaxanthin color, and red yeast red color were left at 100°C for 1 hour. This is a drawing taken afterward.
Figure 5 is a photograph of plant-based hamburger patties manufactured and heated according to Examples 2 to 6, Comparative Examples 1 to 12, and Comparative Examples 22 to 23.
6 shows samples prepared according to Examples 1 to 2, Comparative Example 7, Comparative Example 8, Comparative Example 13, Comparative Example 16, Comparative Example 17, Comparative Example 18, Comparative Example 19, Comparative Example 20, and Comparative Example 21. This is a photo of a plant-based hamburger patty.
Figure 7 is a photograph of the manufacturing process of vegetable hamburger patties (Example 7, Comparative Examples 24 to 27) according to the coloring method.
Figure 8 is a photograph taken of the vegetable hamburger patties of Example 7 and Comparative Examples 24 to 27 before heating.

이하, 본 명세서에 대하여 상세히 설명한다.Hereinafter, this specification will be described in detail.

본 발명의 일 측면은 하기 단계를 포함하는 식물성 햄버거 패티 제조방법에 관한 것이다.One aspect of the present invention relates to a method for producing plant-based hamburger patties comprising the following steps.

a) 식물성 조직 단백질(TVP), 홍국균 분말 및 증류수를 혼합하여 식물성 조직 단백질을 수화시키는 단계; a) mixing vegetable tissue protein (TVP), red yeast rice powder, and distilled water to hydrate the vegetable tissue protein;

b) 상기 a) 단계에서 제조된 수화된 식물성 조직 단백질; 비트 색소, 락색소 또는 이들의 혼합물; 및 식물성 오일;을 혼합하여 반죽 및 성형하는 단계; 및b) hydrated vegetable tissue protein prepared in step a) above; Beet pigment, lac pigment, or mixtures thereof; and vegetable oil; kneading and molding the mixture; and

c) 상기 b) 단계의 성형한 식물성 햄버거 패티 조성물을 열처리하여 식물성 햄버거 패티를 제조하는 단계.c) producing a vegetable hamburger patty by heat-treating the vegetable hamburger patty composition formed in step b).

먼저, a) 식물성 조직 단백질(TVP), 홍국균 분말 및 증류수를 혼합하여 식물성 조직 단백질을 수화시킨다.First, a) hydrate the vegetable tissue protein by mixing vegetable tissue protein (TVP), red yeast rice powder, and distilled water.

식물성 조직 단백질(TVP, textured or texturized vegetable protein)은 식물성 오일의 부생성물(by-product)인 지방이 제거된 대두 등의 원료 가루로부터 제조되는 육류 대체물이다. 본 발명의 식물성 조직 단백질은 특별히 이에 제한되지 않으나, 바람직하게는 조직밀단백질, 조직대두단백질일 수 있고, 조직대두단백질(Textured Soy Protein)은 신속한 요리가 가능하며 높은 단백질 함량과 낮은 지방 함량이 특징이다. 조직대두단백질은 압출 쿠킹(extrusion cooking)으로 알려진 공정을 통해 제조될 수 있다. 예를 들어, 고(high) PDI (Protein Dispersibility Index, 단백질 분산지수)의 탈지 대두가루 및 물을 혼합 실린더와 같은 장치를 이용하여 혼합하여 반죽을 제조하고, 이를 스크류 타입의 압출기의 원통을 통과시켜 제조할 수 있다. Vegetable tissue protein (TVP, textured or texturized vegetable protein) is a meat substitute manufactured from raw material powder such as soybeans from which fat, which is a by-product of vegetable oil, has been removed. The vegetable tissue protein of the present invention is not particularly limited thereto, but is preferably a textured wheat protein or a textured soy protein. Textured soy protein can be cooked quickly and is characterized by high protein content and low fat content. am. Tissue soy protein can be manufactured through a process known as extrusion cooking. For example, dough is prepared by mixing high PDI (Protein Dispersibility Index) defatted soybean powder and water using a device such as a mixing cylinder, and then passing it through the cylinder of a screw-type extruder. It can be manufactured.

상기, a) 단계는 식물성 조직 단백질을 수화하는 과정으로, 식물성 조직 단백질(TVP)와 홍국균 분말 및 증류수를 넣고, 30 분 내지 1 시간동안 수화시키는 것일 수 있다.Step a) is a process of hydrating vegetable tissue protein, and may include adding vegetable tissue protein (TVP), red yeast rice powder, and distilled water, and hydrating for 30 minutes to 1 hour.

이때, 식물성 조직 단백질(TVP) 전체 100 중랑부를 기준으로, 상기 홍국균 분말이 0.01 내지 0.03 중량부 혼합되는 것일 수 있고, 상기 홍국균 분말이 0.01 중량부 미만으로 혼합되거나, 0.03 중량부를 초과하여 혼합되는 경우, 동물성 햄버거 패티와 색감이나 향이 전혀 유사하지 않을 뿐만 아니라, 원료에서 기인하는 특유의 이질적 색감 및 이취가 나타나므로, 소비자들의 선호도를 만족시키지 못하는 문제가 발생할 수 있다.At this time, based on 100 parts by weight of the total vegetable tissue protein (TVP), 0.01 to 0.03 parts by weight of the Hong Yeast fungus powder may be mixed, and if the Hong Yeast fungus powder is mixed in less than 0.01 parts by weight or more than 0.03 parts by weight. , not only is it not at all similar in color or flavor to animal hamburger patties, but also has unique, heterogeneous colors and off-flavors resulting from the raw materials, which may lead to problems of not satisfying consumers' preferences.

상기 증류수는 식물성 조직 단백질(TVP) 중량대비 1.0 내지 5배 혼합되는 것일 수 있다.The distilled water may be mixed at 1.0 to 5 times the weight of vegetable tissue protein (TVP).

다음으로, 상기 a) 단계에서 제조된 수화된 식물성 조직 단백질(TVP), 비트 색소 또는 락색소 및 식물성 오일을 혼합하여 반죽 및 성형한다.Next, the hydrated vegetable tissue protein (TVP) prepared in step a), beet pigment or lac pigment, and vegetable oil are mixed, kneaded, and molded.

상기 a) 단계를 통해 수화된 식물성 조직 단백질(TVP)를 믹서에 넣고, 비트 색소 또는 락색소 및 식물성 오일을 넣은 후, 1 내지 30분간 혼합한다. Put the vegetable tissue protein (TVP) hydrated through step a) into a mixer, add beet color or lac color and vegetable oil, and mix for 1 to 30 minutes.

본 단계에서, 수화된 식물성 조직 단백질(TVP)에 비트 색소 또는 락색소를 분말 상태로 단독으로 혼합할 경우, 색감이 탁해지거나, 균일하게 패티 내, 외부에 퍼지지 않아, 식물성 조직 단백 원료 특유의 색감과 조직감이 노출되는 문제가 발생할 수 있다. In this step, when beet pigment or lac pigment is mixed alone in powder form with hydrated vegetable tissue protein (TVP), the color becomes cloudy or does not spread uniformly inside and outside the patty, resulting in a color unique to the vegetable tissue protein raw material. Problems with over-texture exposure may occur.

상술한 문제가 발생한 경우에는, 색소를 처리하기 이전이나 이후 식물성 오일을 혼합하는 단계를 추가한다고 하더라도 회복되지 못하고, 오히려 식물성 조직 단백질의 결착력만 저하시켜, 패티 성형이 불가능해지는 문제가 발생할 수 있다.When the above-mentioned problem occurs, even if the step of mixing vegetable oil is added before or after coloring treatment, it cannot be recovered. Rather, the binding force of the vegetable tissue protein is reduced, making patty forming impossible.

상기 b) 단계에서, 식물성 조직 단백질(TVP) 전체 100 중랑부를 기준으로, 상기 비트 색소 또는 락색소 0.2 내지 0.6 중량부, 식물성 오일 5 내지 15 중량부 혼합될 수 있는데, 상기 비트 색소 또는 락색소와 식물성 오일의 혼합 중량비가 상기 범위 미만이거나 초과하면, 추후 실험예에 언급된 바와 같이 적색 또는 황색 또는 갈색 빛이 강해져, 동물성 패티와 유사한 색감과 식감을 나타낼 수 없게 되고, 소비자로 하여금 식물성 조직단백질 특유의 이질적 색감이 나타나 기호도가 현저히 저하되는 문제가 발생할 수 있다.In step b), based on 100 total parts of vegetable tissue protein (TVP), 0.2 to 0.6 parts by weight of the beet coloring or rock coloring and 5 to 15 parts by weight of vegetable oil may be mixed with the beet coloring or rock coloring. If the mixing weight ratio of vegetable oil is less than or exceeds the above range, as mentioned in later experimental examples, the red, yellow, or brown color becomes stronger, making it impossible to display a color and texture similar to an animal patty, and making consumers feel that the vegetable tissue protein is unique. A problem may occur where heterogeneous colors appear and preference is significantly reduced.

상기 식물성 오일은 대두유, 카놀라 오일, 코코넛 오일, 올리브 오일 및 팜 오일로 이루어진 군으로부터 선택되는 어느 하나 이상일 수 있으나, 본 발명의 식물성 햄버거 패티의 조성에 따른 물성과 경도를 고려하여 코코넛 오일을 사용하는 것이 가장 바람직하다. 즉, 코코넛 오일을 사용하는 것이 식물성 햄버거 패티의 물성과 경도를 최대로 끌어올릴 수 있다.The vegetable oil may be any one or more selected from the group consisting of soybean oil, canola oil, coconut oil, olive oil, and palm oil. However, considering the physical properties and hardness according to the composition of the vegetable hamburger patty of the present invention, coconut oil is used. It is most desirable. In other words, using coconut oil can maximize the physical properties and hardness of plant-based hamburger patties.

상기 b) 단계에, 상기 식물성 조직 단백질(TVP) 전체 100 중량부를 기준으로, 카라기난 0.5 내지 5 중량부, 알긴산 0.5 내지 5 중량부, 분리대두단백 0.5 내지 5 중량부, 밀식이섬유 0.5 내지 5 중량부, 소금 1 내지 2 중량부, 인산염 0.1 내지 1 중량부, MSG 0.1 내지 0.5 중량부 및 후추 0.001 내지 0.005 중량부를 더 첨가할 수 있다. 상기 첨가물의 추가를 통해, 식물성 햄버거 패티 특유의 이미와 이취를 저감함과 동시에 쇠고기 패티와 유사한 관능적인 효과를 나타낼 수 있다.In step b), based on 100 parts by weight of the total vegetable tissue protein (TVP), 0.5 to 5 parts by weight of carrageenan, 0.5 to 5 parts by weight of alginic acid, 0.5 to 5 parts by weight of isolated soy protein, and 0.5 to 5 parts by weight of wheat dietary fiber. parts, 1 to 2 parts by weight of salt, 0.1 to 1 part by weight of phosphate, 0.1 to 0.5 parts by weight of MSG, and 0.001 to 0.005 parts by weight of pepper may be further added. Through the addition of the above additives, the unique taste and off-flavor of vegetable hamburger patties can be reduced and at the same time, a sensory effect similar to that of beef patties can be achieved.

상기 c) 가열처리 단계는, 햄버거 패티를 제조하는 가열조건이라면 특별히 이에 제한되지 않으나, 동물성 햄버거 패티와 유사한 색상을 내기 위해서는 100 ℃에서 5 내지 10분간 가열하는 것이 바람직하다.The heat treatment step c) is not particularly limited as long as it is a heating condition for producing hamburger patties, but it is preferable to heat at 100° C. for 5 to 10 minutes in order to produce a color similar to that of an animal hamburger patty.

본 발명에 따른 식물성 햄버거 패티는 조리 전뿐만 아니라 조리 후에도, 색감과 식감이 동물성 햄버거 패티와 매우 흡사하며, 종래 식물성 햄버거 패티와도 유사한 색도와 우수한 기호도를 나타냄을 확인하였다.It was confirmed that the vegetable hamburger patty according to the present invention has a color and texture very similar to that of an animal-based hamburger patty, not only before cooking but also after cooking, and exhibits a color similar to that of a conventional vegetable hamburger patty and excellent preference.

상기 c) 단계 후에, 상기 식물성 햄버거 패티의 겉면에 동물성 햄버거 패티 특유의 갈색빛을 더하기 위해, d) 상기 식물성 햄버거 패티의 겉면에 카라멜라이징된 양파를 바르는 단계;를 더 포함할 수 있다.After step c), the step d) of applying caramelized onions to the outside of the vegetable hamburger patty may be further included in order to add brown color unique to the animal hamburger patty to the outside of the vegetable hamburger patty.

상기 식물성 햄버거 패티는 육류 및 기타 색조를 포함하지 않음에도 불구하고, 조직감이 우수하고, 이취가 나지 않으면서도 자연스러운 육색을 구현할 수 있어, 종래 동물성 햄버거 패티와 동등한 수준의 외형을 갖는다. 또한, 종래 식물성 햄버거 패티인 임파서블 버거, 비욘드 버거와 동등 수준 이상의 기호도를 갖는 것일 수 있다.Although the plant-based hamburger patty does not contain meat or other colorants, it has an excellent texture and can achieve natural meat color without producing an off-flavor, so it has an appearance equivalent to that of a conventional animal-based hamburger patty. In addition, it may have a preference level at or above the same level as that of the Impossible Burger and Beyond Burger, which are conventional plant-based hamburger patties.

본 발명의 다른 측면은 상기 제조방법으로 제조된 식물성 햄버거 패티를 제공한다.Another aspect of the present invention provides a vegetable hamburger patty produced by the above production method.

본 발명의 또 다른 측면은 상기 식물성 햄버거 패티를 포함하는 식품을 제공한다.Another aspect of the present invention provides a food comprising the plant-based hamburger patty.

이하, 바람직한 실시예를 들어 본 발명을 더욱 상세하게 설명한다. 그러나 이들 실시예는 본 발명을 보다 구체적으로 설명하기 위한 것으로, 본 발명의 범위가 이에 의하여 제한되지 않는다는 것은 당업계의 통상의 지식을 가진 자에게 자명할 것이다.Hereinafter, the present invention will be described in more detail with reference to preferred embodiments. However, these examples are for illustrating the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited thereto.

<실시예 1-6 및 비교예 1-23> 식물성 햄버거 패티의 제조<Example 1-6 and Comparative Example 1-23> Production of vegetable hamburger patties

식물성 햄버거 패티 제조 시, 패티 무게에 따른 비율로 색소를 첨가하였다. 식물성 햄버거 패티는 수화 TVP(texturized vegetable protein) 100 g, 코코넛유 10 g, 카라기난 1 g, 알긴산 1 g, 분리대두단백 1 g, 밀식이섬유 TG 1 g, 소금 1.5 g, 인산염 0.50 g, MSG 0.30 g, 후추 0.002 g으로 제조하였다. 제조한 식물성 햄버거 패티에 하기 표 1에 도시한 천연색소를 첨가하였다.When manufacturing plant-based hamburger patties, coloring was added at a ratio based on the patty weight. The vegetable hamburger patty contains 100 g of hydrated TVP (texturized vegetable protein), 10 g of coconut oil, 1 g of carrageenan, 1 g of alginic acid, 1 g of soy protein isolate, 1 g of wheat dietary fiber TG, 1.5 g of salt, 0.50 g of phosphate, and 0.30 MSG. g, prepared with 0.002 g of pepper. Natural pigments shown in Table 1 below were added to the prepared vegetable hamburger patties.

구분division 패티patty 색소pigment 홍국균Hong Guk-kyun 비트beat 아스타잔틴Astaxanthin 자색고구마Purple sweet potato 파프리카paprika 락색소Rock pigment 대조군control group 100 g100 g -- -- -- -- -- -- 실시예 1Example 1 100 g100 g 0.01 g0.01g 0.2 g0.2g -- -- -- -- 실시예 2Example 2 100 g100g 0.03 g0.03g 0.6 g0.6g -- -- -- -- 실시예 3Example 3 100 g100g 0.01 g0.01g -- -- -- -- 0.2 g0.2g 실시예 4Example 4 100 g100g 0.01 g0.01g -- -- -- -- 0.6 g0.6g 실시예 5Example 5 100 g100 g 0.03 g0.03g -- -- -- -- 0.2 g0.2g 실시예 6Example 6 100 g100g 0.03 g0.03g -- -- -- -- 0.6 g0.6g 비교예 1Comparative Example 1 100 g100g 0.1 g0.1g -- -- -- -- -- 비교예 2Comparative Example 2 100 g100g -- 0.6 g0.6g -- -- -- -- 비교예 3Comparative Example 3 100 g100 g -- -- 0.1 g0.1g -- -- -- 비교예 4Comparative Example 4 100 g100 g -- -- -- 0.1 g0.1g -- -- 비교예 5Comparative Example 5 100 g100g -- -- -- -- 0.6 g0.6g -- 비교예 6Comparative Example 6 100 g100g 0.005 g0.005 g -- -- -- -- -- 비교예 7Comparative Example 7 100 g100g 0.01 g0.01g -- -- -- -- -- 비교예 8Comparative Example 8 100 g100g 0.03 g0.03g -- -- -- -- -- 비교예 9Comparative Example 9 100 g100 g 0.05 g0.05 g -- -- -- -- -- 비교예 10Comparative Example 10 100 g100 g -- -- -- -- -- 0.1 g0.1g 비교예 11Comparative Example 11 100 g100 g -- -- -- -- -- 0.2 g0.2g 비교예 12Comparative Example 12 100 g100 g -- -- -- -- -- 0.6 g0.6g 비교예 13Comparative Example 13 100 g100 g 0.03 g0.03g -- 0.06 g0.06 g -- -- -- 비교예 14Comparative Example 14 100 g100 g 0.03 g0.03g -- -- 0.06 g0.06 g -- -- 비교예 15Comparative Example 15 100 g100 g 0.03 g0.03g -- -- -- 0.6 g0.6g -- 비교예 16Comparative Example 16 100 g100g 0.01 g0.01g -- 0.02 g0.02g -- -- -- 비교예 17Comparative Example 17 100 g100g 0.01 g0.01g -- -- 0.02 g0.02g -- -- 비교예 18Comparative Example 18 100 g100g -- 0.6 g0.6g 0.02 g0.02g -- -- -- 비교예 19Comparative Example 19 100 g100 g -- 0.2 g0.2g -- -- -- -- 비교예 20Comparative Example 20 100 g100 g -- 0.5 g0.5g -- -- -- -- 비교예 21Comparative Example 21 100 g100g -- 0.5 g0.5g -- 0.2 g0.2g -- -- 비교예 22Comparative Example 22 100 g100 g 0.08 g0.08g -- -- -- -- 0.2 g0.2g 비교예 23Comparative Example 23 100 g100g 0.08 g0.08g -- -- -- -- 0.6 g0.6g

<실시예 7 및 비교예 24-27> 제조방법에 따른 식물성 햄버거 패티의 제조.<Example 7 and Comparative Examples 24-27> Production of vegetable hamburger patties according to the manufacturing method.

식물성 햄버거 패티를 제조하는 방법에 따라, 색감과 기호도를 평가하기 위하여, 다음과 같이 다양하게 색소를 첨가하여 식물성 햄버거 패티를 제조하였다.According to the method of producing vegetable hamburger patties, in order to evaluate color and preference, vegetable hamburger patties were manufactured by adding various pigments as follows.

먼저, 조직화된 대두단백(TVP) 100 g을 ㈜호경테크로부터 구입한 후, 물에 담궈 10분씩 3회로 나누어 물기를 짠 후, 30분간 제1 색소를 혼합하였다. 이렇게 수화 TVP를 다시 물에 4번 짠 후 믹서기로 세절한 후, 여기에 제2 색소 10 g를 혼합하였다. 상기 혼합물에 카라기난 1 g, 알긴산 1 g, 분리대두단백 1 g, 밀식이섬유 TG 1 g, 소금 1.5 g, 인산염 0.5 g, MSG 0.3 g, 후추 0.002 g을 첨가한 후, 반죽하여 식물성 햄버거 패티를 제조하였다. First, 100 g of organized soy protein (TVP) was purchased from Hokyung Tech Co., Ltd., soaked in water, divided into three times for 10 minutes each, squeezed out the water, and then mixed with the first pigment for 30 minutes. The hydrated TVP was squeezed in water again four times, cut into pieces in a blender, and then 10 g of the second dye was mixed with it. Add 1 g of carrageenan, 1 g of alginic acid, 1 g of soy protein isolate, 1 g of wheat dietary fiber TG, 1.5 g of salt, 0.5 g of phosphate, 0.3 g of MSG, and 0.002 g of pepper to the mixture, then knead to make a vegetable hamburger patty. Manufactured.

이때, 비교예 24는 분말상태의 색소를 바로 처리하여 수화 TVP를 제조하였고, 이를 제외하고는 모두 실시예 7과 동일하게 제조하였다.At this time, in Comparative Example 24, hydrated TVP was manufactured by directly processing the powdered pigment, and was manufactured in the same manner as Example 7 except for this.

본 발명에서 홍국균 분말은 다음과 같이 제조한 것을 사용하였다. 홍국균 표준균주인 모나스커스 앙카(Monascus anka, KCTC6121)를 한국생명공학연구원 유전자원센터 유전자은행에서 분양받은 후, 이를 PDA배지를 사용하여 30℃에서 4일간 배양한 후, 액체배양에 사용하였으며, PDA에서 agar를 뺀 PDB 액체배지 50㎖에 상기 PDA배지로 배양한 균주를 접종하여 30℃에서 10일간 진탕배양한 후, 이를 50 ℃에서 5 시간동안 열풍건조하고 후드믹서로 분쇄한 다음 100 mesh 체를 통과시켜 홍국균 분말을 제조하였다(도 1).In the present invention, red yeast powder was used prepared as follows. Monascus anka (KCTC6121), a standard strain of Hong Guk-bun, was purchased from the gene bank of the Korea Research Institute of Bioscience and Biotechnology, Genetic Resources Center, and cultured for 4 days at 30°C using PDA medium, then used for liquid culture. The strain cultured with the PDA medium was inoculated into 50 ml of PDB liquid medium minus the agar, cultured with shaking at 30°C for 10 days, dried with hot air at 50°C for 5 hours, pulverized with a hood mixer, and sieved through a 100 mesh sieve. Red yeast powder was prepared by passage (Figure 1).

구분division 제1 색소primary pigment 제2 색소secondary pigment 색소pigment water 코코넛 오일coconut oil 색소pigment 실시예 7Example 7 홍국균 분말
0.06 g
Red yeast powder
0.06 g
100 mL100mL 42.6 g42.6 g 비트분말 0.3 g0.3 g of beet powder
비교예 24Comparative Example 24 홍국균 분말
0.06 g
Red yeast powder
0.06 g
-- -- 비트분말 0.3 g0.3 g of beet powder
비교예 25Comparative Example 25 비트분말
0.3 g
beet powder
0.3g
100 mL100mL -- 홍국균 분말 0.06 gRed yeast powder 0.06 g
비교예 26Comparative Example 26 홍국균 분말
0.06 g
Red yeast powder
0.06 g
100 mL100mL -- 비트분말 0.3 g0.3 g of beet powder
비교예 27Comparative Example 27 비트분말
0.3 g
beet powder
0.3g
100 mL100mL 42.6 g42.6 g 홍국균 분말 0.06 gRed yeast powder 0.06 g
비교예 28Comparative Example 28 홍국균 분말 0.06 g
+
비트분말 0.3 g
Red yeast powder 0.06 g
+
0.3 g of beet powder
100 mL100mL 42.6 g42.6 g --
비교예 29Comparative Example 29 -- 100 mL100mL 42.6 g42.6 g 홍국균 분말 0.06 g
+
비트분말 0.3 g
Red yeast powder 0.06 g
+
0.3 g of beet powder

<실시예 8-11 및 비교예 30-31> 홍국균색소와 락색소를 첨가하여 제조된 식물성 햄버거 패티의 제조.<Example 8-11 and Comparative Example 30-31> Preparation of a vegetable hamburger patty prepared by adding red yeast color and lac pigment.

실시예 7에서 홍국균 분말과 비트분말 대신, 홍국균색소와 락색소를 제1 색소와 제2 색소로써 하기 표 3에 따른 다양한 조성으로 첨가한 것을 제외하고는 모두 실시예 7과 동일하게 하여 식물성 햄버거 패티를 제조하였다. Vegetable hamburger patties were prepared in the same manner as in Example 7, except that instead of the red yeast powder and beet powder, red yeast color and lacquer pigment were added as first and second colorants in various compositions according to Table 3 below. was manufactured.

구분division 제1 색소primary pigment 제2 색소secondary pigment 색소pigment water 코코넛 오일coconut oil 색소pigment 실시예 8Example 8 홍국균 분말
0.001 g
Red yeast powder
0.001 g
100 mL100mL 42.6 g42.6 g 락색소 분말
0.2 g
Rock pigment powder
0.2g
실시예 9Example 9 홍국균 분말
0.001 g
Red yeast powder
0.001 g
100 mL100mL 42.6 g42.6 g 락색소 분말
0.6 g
Rock pigment powder
0.6g
실시예 10Example 10 홍국균 분말
0.003 g
Red yeast powder
0.003 g
100 mL100mL 42.6 g42.6 g 락색소 분말
0.2 g
Rock pigment powder
0.2g
실시예 11Example 11 홍국균 분말
0.003 g
Red yeast powder
0.003 g
100 mL100mL 42.6 g42.6 g 락색소 분말
0.6 g
Rock pigment powder
0.6g
비교예 28Comparative Example 28 홍국균 분말
0.008 g
Red yeast powder
0.008 g
100 mL100mL 42.6 g42.6 g 락색소 분말
0.2 g
Rock pigment powder
0.2g
비교예 29Comparative Example 29 홍국균 분말
0.008 g
Red yeast powder
0.008 g
100 mL100mL 42.6 g42.6 g 락색소 분말
0.6 g
Rock pigment powder
0.6g

<실험예 1> 열에 의한 천연색소의 안정성 평가<Experimental Example 1> Evaluation of the stability of natural pigments by heat

퍼플그레이프색소, 비트색소, 적양배추색소, 액상 적양배추색소, 포도과피추출색소, 락색소, 적색 40호, 아스타잔틴색소, 홍국적색소를 ES식품원료로부터 구입하였다.Purple grape color, beet color, red cabbage color, liquid red cabbage color, grape skin extract color, lac color, red No. 40, astaxanthin color, and red yeast color were purchased from ES Food Ingredients.

홍국균은 홍국균 표준균주인 모나스커스 앙카(Monascus anka, KCTC6121)를 한국생명공학연구원 유전자원센터 유전자은행에서 분양받은 후, 이를 PDA배지를 사용하여 30 ℃에서 4 일간 배양한 후, 액체배양에 사용하였으며, PDA에서 agar를 뺀 PDB 액체배지 50㎖에 상기 PDA배지로 배양한 균주를 접종하여 30 ℃에서 10 일간 진탕배양한 후, 이를 50 ℃에서 5 시간동안 열풍건조하고 후드믹서로 분쇄한 다음 100 mesh 체를 통과시켜 홍국균 분말을 제조한 것을 색소분말로 사용하였다.For Hong Guk-Guk, Monascus anka (KCTC6121), a standard strain of Hong Guk-Guk, was purchased from the Gene Bank of the Korea Research Institute of Bioscience and Biotechnology, Genetic Resource Center, and cultured for 4 days at 30°C using PDA medium before being used in liquid culture. , 50 ml of PDB liquid medium minus the agar from PDA was inoculated with the strain cultured with the PDA medium, cultured with shaking at 30 ℃ for 10 days, dried with hot air at 50 ℃ for 5 hours, pulverized with a hood mixer, and then mixed with 100 mesh. Red yeast powder was prepared by passing it through a sieve and used as a pigment powder.

상기 천연색소에 대한 열에 의한 안정성을 평가하고자 하였다. 각 색소의 수용액(0.01-2.0%)을 조제한 후, 30 ℃, 60 ℃, 100 ℃에서 300 분간 저장하면서 60 분 간격으로 각 색소들의 흡광도를 분광광도계(spectrophotometer)로 측정하였다.We sought to evaluate the thermal stability of the natural pigment. After preparing an aqueous solution (0.01-2.0%) of each pigment, it was stored at 30°C, 60°C, and 100°C for 300 minutes, and the absorbance of each pigment was measured with a spectrophotometer at 60-minute intervals.

도 2 내지 4는 퍼플그레이프색소, 비트색소, 적양배추색소, 액상적양배추, 포도과피추출색소, 락색소, 홍국색소, 적색 40호, 아스타잔틴색소 및 홍국적색소를 30 ℃, 60 ℃, 100 ℃에서 1 시간동안 방치한 후, 촬영한 도면이다.Figures 2 to 4 show purple grape coloring, beet coloring, red cabbage coloring, liquid red cabbage, grape skin extract coloring, lacquering coloring, red yeast coloring, red No. 40, astaxanthin coloring and red yeast coloring at 30°C, 60°C, This is a picture taken after being left at 100°C for 1 hour.

도 2 내지 4에 따르면, 퍼플그레이프색소는 가열온도가 높아질수록 자주색에서 갈색 자색으로, 비트색소는 붉은색에서 탁한 갈색으로 변하는 것을 확인하였다. 홍국색소는 색이 연해지기는 하였으나, 비교적 안정함을 확인하였다.According to Figures 2 to 4, it was confirmed that the purple grape pigment changed from purple to brown-purple as the heating temperature increased, and the beet pigment changed from red to cloudy brown. Although the red yeast pigment became lighter in color, it was confirmed to be relatively stable.

또한, 적양배추색소, 액상적양배추색소 역시, 온도가 높아짐에 따라 점점 색이 연한 노란색으로 크게 변화하였다. 홍국색소, 홍국적색소, 락색소 및 아스타잔틴색소는 열에 의해 색이 연해지기는 하였으나, 비교적 안정하였다. 비트색소, 자색고구마색소, 복분자색소, 포도과피추출색소는 온도가 높아질수록 연한노란색 또는 짙은 갈색/자주색으로 크게 변화함을 확인하였다.In addition, red cabbage coloring and liquid red cabbage coloring also gradually changed to light yellow as the temperature increased. Red yeast pigment, red yeast pigment, lacquer pigment, and astaxanthin pigment were relatively stable, although their colors became lighter due to heat. It was confirmed that beet pigment, purple sweet potato pigment, bokbunja pigment, and grape skin extract pigment changed significantly to light yellow or dark brown/purple as the temperature increased.

상기 천연색소들을 다양한 pH 조건의 Macllvaine 완충용액에 첨가하였을 때, 각 경우에 대한 400-800 nm 범위에서 흡광도 변화를 측정하여 최대 흡수파장을 분석하였다. 그 결과 홍국색소(490 nm), 적색 40호(460 nm) 및 아스타잔틴색소(450 nm)는 pH 7.0에서 최대 흡수 스펙트럼을 나타내었고, 비트색소는 pH 6.0에서 최대 흡수 스펙트럼(480 nm)을 나타내었다.When the natural pigments were added to Macllvaine buffer solution under various pH conditions, the change in absorbance was measured in the range of 400-800 nm for each case and the maximum absorption wavelength was analyzed. As a result, red yeast pigment (490 nm), red No. 40 (460 nm), and astaxanthin pigment (450 nm) showed the maximum absorption spectrum at pH 7.0, and beet pigment showed the maximum absorption spectrum (480 nm) at pH 6.0. indicated.

<실험예 2> 식물성 햄버거 패티의 색감<Experimental Example 2> Color of plant-based hamburger patty

식물성 햄버거 패티 제조 시, 실험예 1을 통해 pH와 열에 안정한 천연색소인 홍국균, 비트, 아스타잔틴을 선별하고, 식물성 고기의 색소로 자주 사용되는 자색고구마, 파프리카 색소를 선택하였다. 실시예 2, 실시예 3, 실시예 4, 실시예 5, 실시예 6, 비교예 1, 비교예 2, 비교예 3, 비교예 4, 비교예 5, 비교예 6, 비교예 7, 비교예 8, 비교예 9, 비교예 10, 비교예 11, 비교예 12, 비교예 22 및 비교예 23에 따라 제조된 식물성 햄버거 패티의 가열 전 후, 색변화를 촬영하여 육안으로 확인하였다. When manufacturing vegetable hamburger patties, natural pigments stable to pH and heat, such as red yeast rice, beets, and astaxanthin, were selected through Experimental Example 1, and purple sweet potato and paprika pigments, which are frequently used as pigments for vegetable meat, were selected. Example 2, Example 3, Example 4, Example 5, Example 6, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, Comparative Example 5, Comparative Example 6, Comparative Example 7, Comparative Example 8, Comparative Example 9, Comparative Example 10, Comparative Example 11, Comparative Example 12, Comparative Example 22, and Comparative Example 23. The color change of the vegetable hamburger patties prepared according to Comparative Example 22 and Comparative Example 23 was photographed and confirmed with the naked eye before and after heating.

도 5는 실시예 2 내지 6, 비교예 1 내지 12 및 비교예 22 내지 23에 따라 식물성 햄버거 패티를 제조하고, 이를 가열한 모습을 촬영한 사진으로, 이에 따르면 패티 100 중량부를 기준으로 홍국균 색소가 0.1 중량부 이상 첨가한 비교예 1의 식물성 햄버거 패티는 색이 너무 과하게 붉었다. 파프리카색소(비교예 5)는 너무 황색을 띄어 고기 패티 색으로는 적절하지 않은 것을 확인하였다. 아스타잔틴색소(비교예 3)의 경우, 황갈색 빛이 과도하게 강하여, 홍국균 색소와 혼합하더라도 붉은빛을 강하게 가져 제품 적용에 무리가 있는 것으로 확인되었다.Figure 5 is a photograph of vegetable hamburger patties prepared and heated according to Examples 2 to 6, Comparative Examples 1 to 12, and Comparative Examples 22 to 23. According to this, the Hong Guk-kyun pigment is based on 100 parts by weight of the patty. The vegetable hamburger patty of Comparative Example 1 added in an amount of 0.1 part by weight or more was excessively red in color. It was confirmed that the paprika pigment (Comparative Example 5) was too yellow and was not appropriate as a meat patty color. In the case of astaxanthin pigment (Comparative Example 3), the yellow-brown color was excessively strong, and even when mixed with the red yeast pigment, it had a strong red color, making it difficult to apply the product.

색소를 단독으로 관찰할 때와는 달리, 실제 식물성 햄버거 패티에 적용하면, 원래 색소의 본연 색과는 큰 차이를 나타나는 것을 확인하였다. 일반 동물성 햄버거 패티와 가장 유사한 색을 나타내는 것은 실시예 1, 실시예 3-6의 식물성 햄버거 패티임을 확인하였다. Unlike when observing the pigment alone, when applied to an actual plant-based hamburger patty, it was confirmed that there was a significant difference from the original color of the original pigment. It was confirmed that the vegetable hamburger patties of Examples 1 and 3-6 had the most similar color to regular animal hamburger patties.

식물성 햄버거 패티 제조시, 패티 100 중량부를 기준으로 홍국균 색소가 0.1 중량부 미만, 0.005 중량부 이상 첨가되는 것이 바람직함을 확인하였다. 또한 홍국균 색소라도 단독으로는 너무 색감이 나타나지 않거나, 분홍 또는 적색의 이질적인 색감을 나타내는 것을 확인하였다. When manufacturing vegetable hamburger patties, it was confirmed that it is preferable to add less than 0.1 parts by weight and more than 0.005 parts by weight of Honggukgyun pigment based on 100 parts by weight of the patty. In addition, it was confirmed that even the red yeast pigment did not show too much color alone or showed a heterogeneous color of pink or red.

종합하면, 홍국균 색소는 비트색소 또는 락색소와 혼합하여 사용하는 것이 가장 바람직함을 알 수 있다.In summary, it can be seen that it is most desirable to use red yeast coloring mixed with beet coloring or lacing coloring.

<실험예 3> 식물성 햄버거 패티의 색감 분석<Experimental Example 3> Color analysis of plant-based hamburger patties

실시예 1 내지 실시예 2, 비교예 7, 비교예 8, 비교예 13, 비교예 16, 비교예 17, 비교예 18, 비교예 19, 비교예 20, 비교예 21에 따라 제조된 식물성 햄버거 패티를 가열하여, 조리 후 제조된 식물성 햄버거 패티를 얻었다. 상기 제조된 식물성 햄버거 패티의 색변화를 육안으로 확인하였다. Vegetable hamburger patties prepared according to Examples 1 to 2, Comparative Example 7, Comparative Example 8, Comparative Example 13, Comparative Example 16, Comparative Example 17, Comparative Example 18, Comparative Example 19, Comparative Example 20, Comparative Example 21 was heated to obtain a vegetable hamburger patty prepared after cooking. The color change of the prepared vegetable hamburger patty was confirmed with the naked eye.

도 6은 실시예 1 내지 실시예 2, 비교예 7, 비교예 8, 비교예 13, 비교예 16, 비교예 17, 비교예 18, 비교예 19, 비교예 20, 비교예 21에 따라 제조된 식물성 햄버거 패티의 사진이다. 도 6에 따르면, 실시예 1, 2의 식물성 햄버거 패티의 경우, 패티 내부 색이 더 뚜렷하게 나타나고 겉면은 갈색 빛이 돌아, 동물성 햄버거 패티와 가장 유사한 색감을 갖는 것으로 확인되었다.6 shows samples prepared according to Examples 1 to 2, Comparative Example 7, Comparative Example 8, Comparative Example 13, Comparative Example 16, Comparative Example 17, Comparative Example 18, Comparative Example 19, Comparative Example 20, and Comparative Example 21. This is a photo of a plant-based hamburger patty. According to Figure 6, in the case of the vegetable hamburger patties of Examples 1 and 2, the interior color of the patties appeared more clearly and the outside of the patties turned brown, confirming that they had a color most similar to that of animal hamburger patties.

<실험예 4> 식물성 햄버거 패티의 관능성 분석<Experimental Example 4> Sensory analysis of vegetable hamburger patties

실시예 1, 실시예 2, 비교예 7, 비교예 8 및 비교예 16에 따라 제조된 식물성 햄버거 패티에 대한 관능성을 평가하는 실험을 진행하였다(n=25). 이의 실험은 식물성 햄버거 패티에 대한 외관, 겉면, 단면 기호도를 7점 척도법으로 측정하여 확인하였다. 적절성 평가(just-about-right; JAR) 문항으로는 겉면색상, 단면색상을 평가하였다. 적절성 평가는 JAR 척도(1= 매우 많이 약함, 3= 적당함, 5 = 매우많이 강함)을 이용하였다. 측정한 결과는 하기 표 4에 나타내었다. An experiment was conducted to evaluate the sensory properties of vegetable hamburger patties prepared according to Examples 1, 2, Comparative Examples 7, 8, and 16 (n=25). This experiment was confirmed by measuring the appearance, surface, and cross-section preference for vegetable hamburger patties using a 7-point scale. The exterior color and cross-section color were evaluated as just-about-right (JAR) questions. Appropriateness was assessed using the JAR scale (1 = very weak, 3 = moderate, 5 = very strong). The measurement results are shown in Table 4 below.

n=25n=25 대조군control group 실시예 1Example 1 실시예 2Example 2 비교예 7Comparative Example 7 비교예 8Comparative Example 8 비교예
16
Comparative example
16
기호도preference 외관Exterior 4.64c 4.64c 6.00a 6.00a 6.05a 6.05a 5.41b 5.41b 5.18bc 5.18 b.c. 5.55ab 5.55ab 겉면exterior 4.73b 4.73 b 5.91a 5.91a 6.14a 6.14a 5.68a 5.68a 5.77a 5.77a 5.68a 5.68a 단면section 4.09d 4.09d 5.41ab 5.41ab 5.68a 5.68a 4.91bc 4.91 b.c. 4.50cd 4.50cd 5.09abc 5.09 abc JARJAR 겉면색상Surface color 2.48C 2.48C 3.18a 3.18a 3.09ab 3.09ab 2.86b 2.86b 3.32a 3.32a 3.05ab 3.05ab Too littleToo little 50.00%50.00% 9.09%9.09% 13.64%13.64% 45.45%45.45% 4.55%4.55% 18.18%18.18% JARJAR 50.00%50.00% 63.64%63.64% 63.64%63.64% 50.00%50.00% 63.64%63.64% 59.09%59.09% Too muchToo much 9.09%9.09% 27.27%27.27% 22.73%22.73% 4.55%4.55% 31.82%31.82% 22.73%22.73% 단면색상Section color 1.73d 1.73d 3.00ab 3.00ab 2.77bc 2.77 b.c. 2.55c 2.55c 3.32a 3.32a 2.73bc 2.73 b.c. Too littleToo little 90.91%90.91% 18.18%18.18% 40.91%40.91% 59.09%59.09% 31.82%31.82% 50.00%50.00% JARJAR 9.09%9.09% 59.09%59.09% 40.91%40.91% 27.27%27.27% 13.64%13.64% 22.73%22.73% Too muchToo much 0.00%0.00% 22.73%22.73% 18.18%18.18% 13.64%13.64% 54.55%54.55% 27.27%27.27%

표 4에 나타난 바와 같이, 소비자 조사 결과, 외관 기호도는 실시예 1와 실시예 2로부터 제조된 식물성 햄버거 패티가 각각 6.00과 6.05점으로 다른 비교군 대비 우위인 것으로 확인되었다(p<005).As shown in Table 4, as a result of the consumer survey, it was confirmed that the vegetable hamburger patties manufactured in Example 1 and Example 2 were superior to other comparison groups in terms of appearance preference, with 6.00 and 6.05 points, respectively (p<005).

겉면 기호도의 경우, 천연색소를 첨가한 식물성 햄버거 패티가 대조군 패티에 비해 모두 높게 나타났으며(p<0.05). 적절성 평가(JAR) 결과, 약 63%의 소비자들이 실시예 1와 실시예 2로부터 제조된 식물성 햄버거 패티의 겉면 색상이 가장 적당하다고 답하였음을 확인하였다. In terms of surface preference, the plant-based hamburger patties with natural coloring were all higher than the control patties (p<0.05). As a result of the suitability assessment (JAR), it was confirmed that about 63% of consumers responded that the outer color of the vegetable hamburger patties prepared in Examples 1 and 2 was the most appropriate.

단면 기호도의 경우, 실시예 1와 실시예 2 및 비교예 16으로부터 제조된 식물성 햄버거 패티가 각각 5.68, 5.41, 5.09 수준으로 다른 비교군에 비해 유의하게 높았으며(p<0.05), 적절성 평가(JAR) 결과 약 59.1%의 소비자들이 실시예 2로부터 제조된 식물성 햄버거 패티의 단면 색상이 가장 적당하다고 평가하였음을 확인하였다. In the case of cross-sectional preference, the plant-based hamburger patties prepared from Examples 1, 2, and Comparative Example 16 were significantly higher than other comparison groups at levels of 5.68, 5.41, and 5.09, respectively (p<0.05), and the suitability evaluation (JAR) ) As a result, it was confirmed that about 59.1% of consumers evaluated the cross-section color of the vegetable hamburger patty prepared in Example 2 as most appropriate.

겉면 색상에 대한 적절성 평가(JAR) 결과, 약 64% 소비자들이 실시예 1와 실시예 2로부터 제조된 식물성 햄버거 패티 색상이 가장 적당하다고 평가하였다.As a result of the suitability assessment (JAR) for the outer color, about 64% of consumers evaluated the color of the vegetable hamburger patties prepared in Examples 1 and 2 as the most appropriate.

특히, 단면 색상에 대한 적절성 평가(JAR) 결과, 약 59% 소비자들이 실시예 2로부터 제조된 식물성 햄버거 패티의 단면 색상이 가장 적당하다고 평가하였다.In particular, as a result of the suitability assessment (JAR) for cross-section color, about 59% of consumers evaluated that the cross-section color of the vegetable hamburger patty prepared in Example 2 was most appropriate.

소비자 25명을 대상으로 천연색소를 첨가한 식물성 햄버거 패티의 소비자 기호도 평가 결과, 실시예 2로부터 제조된 식물성 햄버거 패티(전체 조성물 100 중량부를 기준으로 홍국 0.03 중량부, 비트 0.6 중량부)의 외관(색상)이 가장 소비자 기호성에 부합하는 것을 확인하였다.As a result of the consumer preference evaluation of vegetable hamburger patties with natural coloring added to 25 consumers, the appearance of the vegetable hamburger patty (0.03 parts by weight of red yeast rice, 0.6 parts by weight of beets, based on 100 parts by weight of the total composition) prepared in Example 2 ( It was confirmed that color) most closely matched consumer preferences.

<실험예 5> 제조방법에 따른 식물성 햄버거 패티의 색도 평가<Experimental Example 5> Color evaluation of vegetable hamburger patties according to manufacturing method

천연색소 첨가 방식에 따라 실시예 7 및 비교예 24 내지 27의 식물성 햄버거 패티를 제조하고, 각각에 대한 색도(L(명도), a(적색도), b(황색도))를 색차계를 사용하여 측정하여 표 4에 나타내었다.Vegetable hamburger patties of Example 7 and Comparative Examples 24 to 27 were prepared according to the method of adding natural coloring, and the chromaticity (L (lightness), a (redness), b (yellowness)) for each was measured using a color difference meter. It was measured and shown in Table 4.

실시예 7의 식물성 햄버거 패티는 TVP 팽윤시킬 때 물에 홍국균을 첨가하여 팽윤시킨 뒤 코코넛 오일에 비트색소를 첨가하여 제조한 것이고, 비교예 24의 식물성 햄버거 패티는 홍국균과 비트를 분말형태로 첨가하여 제조한 것이며, 비교예 25는 TVP 팽윤 시킬 때 물에 비트색소를 첨가하여 팽윤 시킨 후 후에 홍국균 분말을 첨가하여 제조한 것이며, 비교예 26은 TVP 팽윤 시킬 때 물에 홍국균을 첨가하여 팽윤 시킨 뒤 코코넛 오일에 비트색소를 넣어 첨가하여 제조한 것이며, 비교예 27은 TVP 팽윤 시킬 때 물에 비트색소를 첨가하여 팽윤 시킨 뒤 코코넛 오일에 홍국균을 넣어 첨가하여 제조한 것이다.The vegetable hamburger patty of Example 7 was prepared by adding red yeast to water during TVP swelling and then adding beet coloring to coconut oil. The vegetable hamburger patty of Comparative Example 24 was prepared by adding red yeast and beets in powder form. Comparative Example 25 was manufactured by adding beet coloring to water to swell the TVP and then adding red yeast powder to it. Comparative Example 26 was manufactured by adding red yeast to water and swelling it with coconut when swelling the TVP. It was manufactured by adding beet coloring to oil, and Comparative Example 27 was manufactured by adding beet coloring to water to swell TVP and then adding red yeast to coconut oil.

도 8은 가열하기 전, 실시예 7 및 비교예 24 내지 27의 식물성 햄버거 패티를 촬영한 사진이다. 1은 비교예 24의 식물성 햄버거 패티이고, 2는 비교예 25의 식물성 햄버거 패티이며, 3은 비교예 26의 식물성 햄버거 패티이며, 4는 비교예 27의 식물성 햄버거 패티이며, 5는 실시예 7의 식물성 햄버거 패티이다.Figure 8 is a photograph taken of the vegetable hamburger patties of Example 7 and Comparative Examples 24 to 27 before heating. 1 is the vegetable hamburger patty of Comparative Example 24, 2 is the vegetable hamburger patty of Comparative Example 25, 3 is the vegetable hamburger patty of Comparative Example 26, 4 is the vegetable hamburger patty of Comparative Example 27, and 5 is the vegetable hamburger patty of Example 7. It is a plant-based hamburger patty.

도 8에 따르면 비교예 24 내지 27의 식물성 햄버거 패티들은 모두 색소가 뭉치고, 생상이 고르게 입혀지지 않음을 확인하였다. 이에 반해 실시예 7의 식물성 햄버거 패티는 색소가 균일한 것을 확인하였다.According to Figure 8, it was confirmed that the pigments of the vegetable hamburger patties of Comparative Examples 24 to 27 were all clumped together and the color was not evenly applied. In contrast, the vegetable hamburger patty of Example 7 was confirmed to have uniform pigmentation.

따라서 실시예 7의 식물성 햄버거 패티 제조방법과 같이, 홍국균 색소를 첨가한 물에 TVP를 수화시키고, 여기에 비트분말을 첨가한 코코넛 오일을 첨가하는 과정이 가장 바람직함을 확인하였다.Therefore, as in the vegetable hamburger patty manufacturing method of Example 7, it was confirmed that the most preferable process was to hydrate TVP in water to which red yeast pigment was added and then add coconut oil to which beet powder was added.

구분division LL aa bb Average±SDAverage±SD RSD(%)RSD(%) Average±SDAverage±SD RSD(%)RSD(%) Average±SDAverage±SD RSD(%)RSD(%) 조리 전 겉면Surface before cooking 비교예 24Comparative Example 24 60.90±3.5760.90±3.57 5.865.86 7.58±0.827.58±0.82 10.7610.76 13.43±1.6813.43±1.68 12.4912.49 비교예 25Comparative Example 25 52.10±3.0752.10±3.07 5.905.90 9.30±0.639.30±0.63 6.776.77 12.20±1.0512.20±1.05 8.598.59 비교예 26Comparative Example 26 51.28±2.6451.28±2.64 5.155.15 14.15±1.7114.15±1.71 12.1112.11 10.80±0.3910.80±0.39 3.573.57 비교예 27Comparative Example 27 59.72±2.7759.72±2.77 4.634.63 6.74±0.506.74±0.50 7.387.38 12.10±0.6712.10±0.67 5.515.51 비교예 28Comparative Example 28 41.25±6.6441.25±6.64 16.1016.10 5.74±1.645.74±1.64 28.5728.57 13.59±0.9713.59±0.97 7.147.14 비교예 29Comparative Example 29 49.55±6.6349.55±6.63 13.3813.38 6.56±1.246.56±1.24 18.9018.90 14.74±1.0714.74±1.07 7.267.26 실시예 7Example 7 53.01±0.8653.01±0.86 1.611.61 10.42±0.2710.42±0.27 2.632.63 10.71±0.2110.71±0.21 1.961.96 조리 후 겉면Outside after cooking 비교예 24Comparative Example 24 32.20±1.6932.20±1.69 5.255.25 13.71±0.1413.71±0.14 1.051.05 13.49±1.1713.49±1.17 8.688.68 비교예 25Comparative Example 25 34.67±1.2634.67±1.26 3.633.63 13.06±1.5013.06±1.50 11.5011.50 13.53±1.6313.53±1.63 12.0412.04 비교예 26Comparative Example 26 38.88±2.2338.88±2.23 5.735.73 12.65±0.7112.65±0.71 5.575.57 16.04±1.2516.04±1.25 7.777.77 비교예 27Comparative Example 27 34.59±2.5434.59±2.54 7.347.34 12.86±0.3812.86±0.38 2.942.94 13.68±1.6413.68±1.64 12.0112.01 비교예 28Comparative Example 28 30.25±4.1130.25±4.11 13.5913.59 14.97±1.7914.97±1.79 11.9611.96 15.41±1.3415.41±1.34 8.708.70 비교예 29Comparative Example 29 31.41±3.8931.41±3.89 12.3812.38 14.89±2.4214.89±2.42 16.2516.25 16.42±1.8716.42±1.87 11.3911.39 실시예 7Example 7 37.42±0.0537.42±0.05 0.140.14 12.74±0.1812.74±0.18 1.431.43 14.89±0.1114.89±0.11 0.710.71 조리 후 단면Cross section after cooking 비교예 24Comparative Example 24 51.40±0.6951.40±0.69 1.341.34 11.72±1.4111.72±1.41 12.0012.00 12.87±0.2912.87±0.29 2.222.22 비교예 25Comparative Example 25 54.33±0.8354.33±0.83 1.521.52 6.56±0.736.56±0.73 11.1411.14 12.66±0.2412.66±0.24 1.911.91 비교예 26Comparative Example 26 52.92±1.9552.92±1.95 3.683.68 10.55±1.3210.55±1.32 12.5512.55 12.96±0.5012.96±0.50 3.893.89 비교예 27Comparative Example 27 56.75±1.2756.75±1.27 2.242.24 5.79±0.315.79±0.31 5.325.32 13.39±0.3713.39±0.37 2.762.76 비교예 28Comparative Example 28 56.34±6.0156.34±6.01 10.6710.67 12.40±2.2112.40±2.21 17.8217.82 14.54±1.1414.54±1.14 7.847.84 비교예 29Comparative Example 29 56.74±5.7856.74±5.78 10.1910.19 11.75±1.8011.75±1.80 15.3215.32 14.70±1.0514.70±1.05 7.147.14 실시예 7Example 7 49.90±0.0749.90±0.07 0.130.13 11.86±0.1711.86±0.17 1.471.47 12.14±0.0912.14±0.09 0.710.71

표 5에 나타난 바와 같이, 조리 전과 조리 후 모두 실시예 7의 식물성 햄버거 패티의 RSD가 안정적임을 알 수 있다. RSD란 상대표준편차로, 분석법의 정밀도를 나타낸다. 종합하면 식물성 햄버거 패티 제조시, 실시예 7과 같이 TVP 팽윤 단계에서 홍국균 분말을 1차 처리하고, 코코넛 오일에 비트색소를 혼합하여, 2차 처리하는 것이 가장 바람직함을 확인하였다. 홍국균과 비트 또는 락색소를 한번에 다 처리할 경우, 순서에 상관없이 색도 뿐만 아니라, 색감의 균일도까지 크게 달라짐을 알 수 있다.As shown in Table 5, it can be seen that the RSD of the vegetable hamburger patty of Example 7 was stable both before and after cooking. RSD is relative standard deviation and indicates the precision of the analysis method. In summary, it was confirmed that when manufacturing vegetable hamburger patties, it is most preferable to first process red yeast rice powder in the TVP swelling step as in Example 7, and then perform secondary treatment by mixing beet coloring with coconut oil. If you process red yeast and beet or rock coloring all at once, you can see that not only the color but also the uniformity of color changes significantly, regardless of the order.

<실험예 6> 시제품과 식물성 햄버거 패티의 색도 평가<Experimental Example 6> Color evaluation of prototype and vegetable hamburger patties

실시예 7로부터 제조된 식물성 햄버거 패티와, 임파서블 버거의 패티, 비욘드 버거의 패티에 대한 색도(L(명도), a(적색도), b(황색도))를 색차계를 사용하여 측정하여 표 5에 나타내었다. 실시예 7의 식물성 햄버거 패티에 카라멜라이징한 양파를 발라준 '카라멜라이징 패티'를 준비하여, 이에 대한 색도도 측정하였다.The chromaticities (L (lightness), a (redness), b (yellowness)) of the vegetable hamburger patties prepared in Example 7, the Impossible Burger patties, and the Beyond Burger patties were measured using a colorimeter and are shown in the table. It is shown in 5. A 'caramelized patty' was prepared by applying caramelized onions to the vegetable hamburger patty of Example 7, and its color was also measured.

조리전 겉면Surface before cooking 조리 후 겉면Outside after cooking 조리 후 단면Cross section after cooking LL aa bb LL aa bb LL aa bb 실시예 7Example 7 53.01±0.8653.01±0.86 10.42±0.2710.42±0.27 10.71±0.4210.71±0.42 37.42±0.0537.42±0.05 12.74±0.1812.74±0.18 14.89±0.1114.89±0.11 49.90±0.0749.90±0.07 11.86±0.1711.86±0.17 12.14±0.0912.14±0.09 카라멜라이징 패티Caramelizing Patty 32.35±2.4832.35±2.48 9.28±0.789.28±0.78 12.17±1.8612.17±1.86 26.28±3.3826.28±3.38 7.91±1.257.91±1.25 9.00±1.849.00±1.84 49.90±0.0749.90±0.07 11.86±0.1711.86±0.17 12.14±0.0912.14±0.09 임파서블버거Impossible Burger 54.55±1.3754.55±1.37 16.11±2.3716.11±2.37 16.00±0.6616.00±0.66 39.60±2.5139.60±2.51 12.11±1.0112.11±1.01 19.90±0.9819.90±0.98 40.61±1.9640.61±1.96 8.12±3.358.12±3.35 19.74±2.9719.74±2.97 비욘드버거Beyond Burger 41.92±0.5041.92±0.50 14.67±0.6714.67±0.67 8.71±0.448.71±0.44 27.85±1.1227.85±1.12 10.85±1.1910.85±1.19 9.85±0.779.85±0.77 35.25±1.9535.25±1.95 19.03±1.3819.03±1.38 11.82±0.6811.82±0.68

표 6에 따르면, 실시예 7의 식물성 햄버거 패티는 겉면의 색도가 임파서블 버거와 유사하였다. 또한, 카라멜라이징한 양파를 바른 실시예 7의 식물성 햄버거 패티는 비욘드 버거와 조리후 겉면이 유사한 색도를 갖는 것을 확인하였다.According to Table 6, the outer color of the vegetable hamburger patty of Example 7 was similar to that of the Impossible Burger. In addition, it was confirmed that the vegetable hamburger patty of Example 7 coated with caramelized onions had a similar color to the Beyond Burger after cooking.

즉, 본 발명에 따른 식물성 햄버거 패티는 종래 상업화에 성공한 '임파서블 버거 및 비욘드 버거'와 색도와 유사한 것을 확인하였다. '임파서블 버거 및 비욘드 버거'는 식물성 햄버거 패티 중에서 맛과 색이 동물성 햄버거 패티와 매우 흡사하여, 전세계적으로 판매되고 있는 제품이다. 따라서 본 발명의 식물성 햄버거 패티 역시 동물성 햄버거 패티와 매우 흡사한 색도를 가짐을 알 수 있다.In other words, it was confirmed that the vegetable hamburger patty according to the present invention was similar in color to the previously commercialized 'Impossible Burger and Beyond Burger'. 'Impossible Burger and Beyond Burger' are plant-based hamburger patties that are very similar in taste and color to animal-based hamburger patties, and are sold worldwide. Therefore, it can be seen that the vegetable hamburger patty of the present invention also has a color very similar to that of the animal hamburger patty.

<실험예 7> 식물성 햄버거 패티의 관능성 평가<Experimental Example 7> Sensory evaluation of plant-based hamburger patties

실시예 7로부터 제조된 식물성 햄버거 패티와, 임파서블 버거의 패티, 비욘드 버거의 패티에 대한 관능성을 평가하였고, 표 7에 나타내었다(n=30). 이의 실험은 식물성 햄버거 패티에 대한 겉면과 단면 기호도를 9점 척도법으로 측정하여 확인하였다. 측정한 결과는 하기 표 7에 나타내었다. The sensory properties of the plant-based hamburger patties prepared in Example 7, the Impossible Burger patties, and the Beyond Burger patties were evaluated and are shown in Table 7 (n=30). This experiment was confirmed by measuring the outer and cross-sectional preferences for vegetable hamburger patties using a 9-point scale. The measurement results are shown in Table 7 below.

조리 전Before cooking 조리 후After cooking 겉면exterior 단면section 겉면exterior 단면section 실시예 7Example 7 5.30±1.88a 5.30± 1.88a 5.87±1.46a 5.87± 1.46a 6.50±1.666.50±1.66 aa 6.57±1.796.57±1.79 aa 임파서블버거Impossible Burger 5.60±1.45a 5.60± 1.45a 5.83±1.26a 5.83± 1.26a 5.10±1.46a 5.10± 1.46a 4.90±1.18a 4.90± 1.18a 비욘드버거Beyond Burger 6.17±1.82a 6.17± 1.82a 5.80±1.77a 5.80± 1.77a 5.30±1.27a 5.30± 1.27a 4.87±1.55b 4.87± 1.55b

표 7에 따르면 식물성 햄버거 패티에 대한 결과를 살펴보면, 조리 전에는 실시예 7의 식물성 햄버거 패티, 임파서블 버거의 패티 및 비욘드 버거의 패티에 대한 기호도 차이가 거의 없었음을 알 수 있다(p<0.05). According to Table 7, looking at the results for the plant-based hamburger patties, it can be seen that there was little difference in preference for the plant-based hamburger patty of Example 7, the Impossible Burger patty, and the Beyond Burger patty before cooking (p<0.05).

이에 반해, 조리 후에는 기호도의 경우, 실시예 7의 식물성 햄버거 패티가 다른 식물성 햄버거 패비 대비 높은 기호도를 갖는 것으로 확인되었다.On the other hand, in terms of preference after cooking, the plant-based hamburger patty of Example 7 was confirmed to have a higher preference compared to other plant-based hamburger patties.

<실험예 8> 홍국균 색소와 락색소를 첨가한 식물성 햄버거 패티의 관능성 평가<Experimental Example 8> Sensory evaluation of vegetable hamburger patties with added Hong Guk-Gyun pigment and lac pigment

실시예 8 내지 11 및 비교예 30, 비교예 31로부터 제조된 식물성 햄버거 패티에 대한 관능성을 평가하였고, 표 8에 나타내었다(n=30). 이의 실험은 식물성 햄버거 패티에 대한 겉면과 단면 기호도를 9점 척도법으로 측정하여 확인하였다. 측정한 결과는 하기 표 8에 나타내었다.The sensory properties of the vegetable hamburger patties prepared from Examples 8 to 11 and Comparative Examples 30 and 31 were evaluated and are shown in Table 8 (n=30). This experiment was confirmed by measuring the outer and cross-sectional preferences for vegetable hamburger patties using a 9-point scale. The measurement results are shown in Table 8 below.

조리 전Before cooking 조리 후After cooking 겉면exterior 단면section 겉면exterior 단면section 실시예 8Example 8 4.59±1.43c 4.59± 1.43c 4.83±1.85c 4.83± 1.85c 6.04±1.40ab 6.04± 1.40ab 5.67±1.49ab 5.67± 1.49ab 실시예 9Example 9 5.72±1.67ab 5.72± 1.67ab 5.83±1.28ab 5.83± 1.28ab 6.19±1.78ab 6.19± 1.78ab 5.96±1.79ab 5.96± 1.79ab 실시예 10Example 10 5.24±1.64bc 5.24±1.64 b.c. 5.07±1.85bc 5.07±1.85 b.c. 5.63±1.52ab 5.63± 1.52ab 5.63±1.45ab 5.63± 1.45ab 실시예 11Example 11 6.21±1.45a 6.21± 1.45a 6.31±1.54a 6.31± 1.54a 6.41±2.02a 6.41± 2.02a 6.30±1.75a 6.30± 1.75a 비교예 30Comparative Example 30 5.52±2.21ab 5.52± 2.21ab 5.48±2.38abc 5.48± 2.38abc 4.67±2.06ab 4.67± 2.06ab 4.63±2.26ab 4.63± 2.26ab 비교예 31Comparative Example 31 5.17±1.26a 5.17± 1.26a 5.41±1.21a 5.41± 1.21a 4.26±1.81b 4.26± 1.81b 4.26±1.48b 4.26± 1.48b

표 8에 따르면 소비자 조사 결과, 실시예 8 내지 11의 식물성 햄버거 패티의 기호도가 유의하게 높았고, 실시에 11의 식물성 햄버거 패티의 기호도가 가장 높은 것으로 확인되었다. According to Table 8, as a result of the consumer survey, the preference for the plant-based hamburger patties of Examples 8 to 11 was significantly high, and the preference for the plant-based hamburger patty of Example 11 was confirmed to be the highest.

조리 전에는 실시예 8의 식물성 햄버거 패티가 가장 기호도가 낮았고, 나머지는 기호가 유사하였으나, 조리 후에 실시예 11의 기호도가 유의적으로 높은 것으로 확인되었다.Before cooking, the plant-based hamburger patty of Example 8 had the lowest preference, and the rest had similar preferences, but after cooking, the preference of Example 11 was confirmed to be significantly higher.

특히, 홍국균 색소와 락색소의 혼합 중량비가 과도하게 높거나, 과도하게 낮은 경우, 적색도가 조리 후에 현저히 낮아지는 문제가 발생함을 확인하였다. 이에 따라 소비자들의 기호도도 조리 후 비교예 30, 31에서 크게 하락함을 알 수 있다.In particular, it was confirmed that when the mixing weight ratio of the red yeast pigment and the lacquer pigment was excessively high or excessively low, a problem occurred in which the redness was significantly lowered after cooking. Accordingly, it can be seen that consumers' preference also decreased significantly in Comparative Examples 30 and 31 after cooking.

Claims (8)

a) 식물성 조직 단백질(TVP), 홍국균 분말 및 증류수를 혼합하여 식물성 조직 단백질을 수화시키는 단계;
b) 상기 a) 단계에서 제조된 수화된 식물성 조직 단백질; 비트 색소 또는 락색소; 및 식물성 오일;을 혼합하여 반죽 및 성형하는 단계; 및
c) 상기 b) 단계의 성형한 식물성 햄버거 패티 조성물을 열처리하여 식물성 햄버거 패티를 제조하는 단계;를 포함하고,
상기 a) 단계에서, 식물성 조직 단백질(TVP) 전체 100 중랑부를 기준으로, 상기 홍국균 분말이 0.01 내지 0.03 중량부 혼합되며,
상기 b) 단계에서, 식물성 조직 단백질(TVP) 전체 100 중랑부를 기준으로, 상기 비트 색소 또는 락색소는 0.2 내지 0.6 중량부, 식물성 오일은 5 내지 15 중량부 혼합되는 것을 특징으로 하는 식물성 햄버거 패티 제조방법.
a) mixing vegetable tissue protein (TVP), red yeast rice powder, and distilled water to hydrate the vegetable tissue protein;
b) hydrated vegetable tissue protein prepared in step a) above; beet pigment or lac pigment; and vegetable oil; kneading and molding the mixture; and
c) heat-treating the vegetable hamburger patty composition formed in step b) to produce a vegetable hamburger patty;
In step a), 0.01 to 0.03 parts by weight of the red yeast fungus powder is mixed, based on 100 total parts of vegetable tissue protein (TVP),
In step b), based on a total of 100 parts of vegetable tissue protein (TVP), 0.2 to 0.6 parts by weight of the beet coloring or rock coloring and 5 to 15 parts by weight of vegetable oil are mixed, producing a vegetable hamburger patty. method.
삭제delete 삭제delete 제1항에 있어서,
상기 식물성 오일은 대두유, 카놀라 오일, 코코넛 오일, 올리브 오일 및 팜 오일로 이루어진 군으로부터 선택되는 어느 하나 이상인 것을 특징으로 하는 식물성 햄버거 패티 제조방법.
According to paragraph 1,
A method for producing a vegetable hamburger patty, wherein the vegetable oil is at least one selected from the group consisting of soybean oil, canola oil, coconut oil, olive oil, and palm oil.
제1항에 있어서,
상기 b) 단계에,
상기 식물성 조직 단백질(TVP) 전체 100 중량부를 기준으로, 카라기난 0.5 내지 5 중량부, 알긴산 0.5 내지 5 중량부, 분리대두단백 0.5 내지 5 중량부, 밀식이섬유 0.5 내지 5 중량부, 소금 1 내지 2 중량부, 인산염 0.1 내지 1 중량부, MSG 0.1 내지 0.5 중량부 및 후추 0.001 내지 0.005 중량부를 더 첨가하는 것을 특징으로 하는 식물성 햄버거 패티 제조방법.
According to paragraph 1,
In step b) above,
Based on 100 parts by weight of the total vegetable tissue protein (TVP), 0.5 to 5 parts by weight of carrageenan, 0.5 to 5 parts by weight of alginic acid, 0.5 to 5 parts by weight of isolated soy protein, 0.5 to 5 parts by weight of wheat dietary fiber, and 1 to 2 parts by weight of salt. Part by weight, a method for producing a vegetable hamburger patty, characterized in that adding 0.1 to 1 part by weight of phosphate, 0.1 to 0.5 part by weight of MSG, and 0.001 to 0.005 part by weight of pepper.
제1항에 있어서,
상기 c) 단계는 100 ℃에서 5 내지 10분간 가열한 것을 특징으로 하는 식물성 햄버거 패티 제조방법.
According to paragraph 1,
Step c) is a method of producing a vegetable hamburger patty, characterized in that heating at 100 ° C. for 5 to 10 minutes.
제1항에 있어서,
상기 c) 단계 후에,
d) 상기 식물성 햄버거 패티의 겉면에 카라멜라이징된 양파를 바르는 단계;를 더 포함하는 것을 특징으로 하는 식물성 햄버거 패티 제조방법.
According to paragraph 1,
After step c) above,
d) applying caramelized onions to the outer surface of the vegetable hamburger patty.
제1항에 따라 제조된 식물성 햄버거 패티.A plant-based hamburger patty manufactured according to claim 1.
KR1020200182081A 2020-12-23 2020-12-23 manufacturing method of vegetarian hamburger patty used natural colorant KR102580636B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020200182081A KR102580636B1 (en) 2020-12-23 2020-12-23 manufacturing method of vegetarian hamburger patty used natural colorant

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020200182081A KR102580636B1 (en) 2020-12-23 2020-12-23 manufacturing method of vegetarian hamburger patty used natural colorant

Publications (2)

Publication Number Publication Date
KR20220091659A KR20220091659A (en) 2022-07-01
KR102580636B1 true KR102580636B1 (en) 2023-09-22

Family

ID=82397203

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020200182081A KR102580636B1 (en) 2020-12-23 2020-12-23 manufacturing method of vegetarian hamburger patty used natural colorant

Country Status (1)

Country Link
KR (1) KR102580636B1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102525119B1 (en) * 2023-02-14 2023-04-25 주식회사 쏘이마루 Method for manufacturing of steak including bean sprout and steak including bean sprout manufactured by the same

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060073261A1 (en) * 2004-10-06 2006-04-06 Mcmindes Matthew K Soy protein containing food product and process for preparing same

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101808534A (en) * 2007-04-05 2010-08-18 索莱有限责任公司 Meat compositions comprising colored structured protein products
KR20200127380A (en) * 2019-05-02 2020-11-11 롯데푸드 주식회사 A manufacturing method of vegetarian hamburger patty reduced unpleasant taste and smell
KR102027091B1 (en) 2019-06-17 2019-10-01 문석우 Process For Preparing A Vegetable Patty Using Outer Leaves Of Greens

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060073261A1 (en) * 2004-10-06 2006-04-06 Mcmindes Matthew K Soy protein containing food product and process for preparing same

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
네이버 블로그(홈메이드 수제버거:육즙가득한 햄버거 패티 만드는 방법과 보관방법, https://blog.naver.com/kjmsos119/222050096793, 2020.08.03.) 사본 1부.*

Also Published As

Publication number Publication date
KR20220091659A (en) 2022-07-01

Similar Documents

Publication Publication Date Title
Olawuyi et al. Quality and antioxidant properties of functional rice muffins enriched with shiitake mushroom and carrot pomace
Çabuk et al. Fortification of traditional egg pasta (erişte) with edible insects: Nutritional quality, cooking properties and sensory characteristics evaluation
Cuj‐Laines et al. Physicochemical properties of ready‐to‐eat extruded nixtamalized maize‐based snacks enriched with grasshopper
KR101349315B1 (en) Bar rice cake containing purple sweet-potato and ginger and manufacturing method thereof
KR102580636B1 (en) manufacturing method of vegetarian hamburger patty used natural colorant
Bashir et al. Preparation of micronutrients fortified Spirulina supplemented Rice‐soy crisps processed through novel supercritical fluid extrusion
Klunklin et al. Addition of defatted green‐lipped mussel powder and mixed spices to wheat–purple rice flour biscuits: Physicochemical, in vitro digestibility and sensory evaluation
Gewehr et al. Nutritional and technological evaluation of bread made with quinoa flakes (Chenopodium quinoa willd)
KR101173517B1 (en) Method of preparing well-being baquette
Meriles et al. Effect of heat‐treated wheat germ on dough properties and crackers quality
Lima et al. Vegetal burgers of cashew fiber and cowpea: formulation, characterization and stability during frozen storage
KR20210048178A (en) Manufacturing method of dumpling skin and dumpling skin manufactured by the same
Ataei Nukabadi et al. Measurement of quercetin and 4‐Hydroxyisoleucine in cakes fortified with nettle and fenugreek seed and study of their physicochemical properties using mixture design approach
Téllez‐Morales et al. The use of tubers in the development of extruded snacks: A review
Conte et al. Italian Dried Pasta: Conventional and Innovative Ingredients and Processing
Khodaei et al. Physicochemical and sensory characteristics of pasta enriched with blue whiting (Micromesistius poutassou) fish protein hydrolysate
US20090162514A1 (en) Production of pasta using rice bran and rice flour
KR101441547B1 (en) Meat chip using less preferred meat parts and meat chip prepared thereby
KR101719207B1 (en) functional cereal foods having component of sweet potato
KR20160113946A (en) A process for the preparation of pizza dough and pizza dough prepared therefrom
KR20170021438A (en) composition for manufacturing fish-shaped bread containing scorched rice made with grains, fish-shaped bread and process for producing the same
Guan et al. Enhancing the nutritional value of bread by the addition of insect powder: a novel class of food protein additives
KR20210069863A (en) A pesto comprising spinach and chamnamul, and a producing method the same
Simanjuntak et al. Sensory and chemical properties of cookies formulated with South Kalimantan’s local comodities
Cho et al. Changes in physical properties of Yukwa (fried rice snack) by soybean extract concentration and incubation time

Legal Events

Date Code Title Description
E902 Notification of reason for refusal
E701 Decision to grant or registration of patent right