KR102553803B1 - Feed additive composition for livestock or fish comprising fermented product of bass and blue gill as effective component and uses thereof - Google Patents

Abstract

The present invention relates to a feed additive composition for livestock or fish containing a fermented product of bass and bluegill as an active ingredient and a use thereof. It can improve the utilization of livestock and fish, and can be used in the livestock and fisheries industries by increasing the productivity and economy of livestock and fish, or used as fishing tackle and bait.

Description

Feed additive composition for livestock or fish comprising fermented product of bass and blue gill as an effective component and uses thereof {Feed additive composition for livestock or fish comprising fermented product of bass and blue gill as effective component and uses thereof}

The present invention relates to a feed additive composition for livestock or fish comprising fermented products of bass and bluegill as an active ingredient and uses thereof.

Conventional feed additives have been prepared to contain antibiotics or antibacterial agents, which are synthetic chemicals, for the purpose of improving the growth rate of livestock or fish, increasing the efficiency of feed, and preventing diseases. However, when these feed additives are used in combination with livestock or fish feed, immunity to diseases is reduced and resistance to antibiotics is increased. In addition, since drugs such as antibiotics remain in the body of livestock or fish, they may affect the health of people who consume them. Accordingly, research and development and efficacy studies of candidate substances with secured stability that can replace antibiotics and antimicrobials for both consumers and producers in the animal industry and fish aquaculture industry are being conducted.

Bass and blue gill are representative exotic species that destroy the ecosystem of lakes and rivers in Korea, and are the first ecosystem disturbing species designated by the Ministry of Environment in 1998. Bass and bluegill have no upper predators in nature, and their populations have continued to increase because they are not used for food due to the recognition that they are noxious species and their unique smell. As foreign introduced fish species such as bass and bluegill are introduced into large dam lakes and rivers, the aquatic ecosystem is frequently disturbed due to the predation of native fish by them.

The government has developed an alien species ecological risk assessment system, established an alien species management database, related interdisciplinary exchanges, developed a preventive management system through public awareness and public awareness development, and established a cooperative system for managing ecosystem disturbing fish species in which all ministries jointly participate. Although integrated management of fish species disturbing the ecosystem is proposed and intensive management is being carried out, damage to fish farms and farmers is increasing due to ecosystem destruction, water pollution and economic problems in places where management is not yet managed. In addition, the high value-added industry using actually caught bass and bluegill is still incomplete, and it is necessary to establish and take measures to utilize it.

Accordingly, the present inventors have developed an economical feed additive containing a fermented product of bass and bluegill fermented with lactic acid bacteria and yeast to increase the efficiency of amino acids in order to eradicate bass and bluegill, which are ecosystem-disturbing species, and to solve the problem of feed cost for livestock farmers and fish farmers. developed.

On the other hand, Korean Patent Publication No. 2021-0088817 discloses an unfermented 'fish feed using bass and bluegill and a method for manufacturing the same', and Korean Patent No. 1923553 discloses a 'bath manufactured through smoke drying and low temperature drying'. 'Powder and method for producing the same' is disclosed, but 'feed additive composition for livestock or fish containing fermented product of bass and bluegill as an active ingredient and use thereof' of the present invention prepared through the process of inoculating and fermenting lactic acid bacteria and yeast ' Nothing is mentioned about it.

The present invention has been derived from the above needs, and the present inventors have found that the powder of bass and bluegill is Bacillus subtilis , Bacillus licheniformis , Lactobacillus plantarum, Lactobacillus plantarum , Lactobacillus casei ( Lactobacillus casei ), Lactobacillus acidophilus ( Lactobacillus acidophilus ) and Saccharomyces genus ( Saccharomyces sp. ) yeast were added and cultured to prepare a fermented product of bath and bluegill, and then drying the fermented product and pulverized to obtain a powder. The present invention was completed by confirming that, as a result of feeding companion animals and ornamental fish with the powder of the fermented bass and bluegill, there was an effect of improving the intestinal environment of companion animals and increasing the productivity of ornamental fish.

In order to solve the above problems, the present invention provides a feed additive composition for livestock or fish comprising fermented products of bass and bluegill as an active ingredient.

In addition, the present invention provides a feed composition for livestock or fish comprising the feed additive composition.

In addition, the present invention provides a fishing catch composition comprising a fermented product of bass and bluegill as an active ingredient.

The present invention can improve the utilization of ecosystem disrupting species by using bass and bluegill, which are alien species that destroy the ecosystem, and will be very useful in the livestock and fisheries industries by increasing the productivity and economy of livestock and fish.

In order to achieve the object of the present invention, the present invention provides a feed additive composition for livestock or fish comprising fermented products of bass and bluegill as an active ingredient.

In the feed additive composition of the present invention, the livestock may be any one selected from the group consisting of dogs, pigs, cows, chickens, horses, goats, ducks, geese, cats and rabbits, preferably dogs and cats. It may be a companion animal, more preferably a dog, but is not particularly limited thereto. In addition, the fish are carp, mandarin fish, halibut, flounder, bass, grouper, black sea bream, red sea bream, stone sea bream, other sea bream, croaker, yellowtail, puffer fish, rockfish, other rockfish, mackerel, yellowtail, trout, It may be mullet, salmon, conger eel, horse mackerel, trout, clam, rodent, sturgeon, eel, catfish, tropical fish, etc., preferably an ornamental fish that can be raised for appreciation or pet among the above fish, It is not particularly limited thereto.

In the method according to one embodiment of the present invention, the feed additive is used to inoculate bass and bluegill bodies with a mixture of strains of the genus Bacillus , strains of the genus Lactobacillus and yeast strains of the genus Saccharomyces It may be prepared by adding an amino acid, fermenting, and then drying. Preferably, a strain of the genus Bacillus , a strain of the genus Lactobacillus , and a yeast strain of the genus Saccharomyces are used in bath and bluegill bodies. After inoculating the mixture and adding amino acids to fermentation, 2-4 hours at 13-17℃, 2-4 hours at 23-27℃, 11-13 hours at 35-39℃ and 11-13 hours at 53-57℃. It may be prepared by sequentially drying for a period of time, and more preferably, a mixture of a Bacillus genus strain, a Lactobacillus genus strain, and a Saccharobacillus genus yeast strain is inoculated into bass and bluegill bodies, and amino acids are added to 28 to 37 ° C. After fermentation for 46~50 hours at 13~17℃ for 2~4 hours, 23~27℃ for 2~4 hours, 35~39℃ for 11~13 hours and 53~57℃ for 11~13 hours It may be prepared by sequentially drying, and more preferably, bass and bluegill fish are mixed in equal amounts, and a mixture of Bacillus strains, Lactobacillus strains and Saccharomyces yeast strains based on the total weight of the fish is inoculated. 1% amino acid was added and incubated at 28-37 ° C for 48 hours, followed by sequential drying at 15 ° C for 3 hours, 25 ° C for 3 hours, 37 ° C for 12 hours and 55 ° C for 12 hours. It may be, but is not limited thereto.

In the feed additive composition of the present invention, the inoculation of the strain mixture is performed by inoculating a strain of the genus Bacillus, a strain of the genus Lactobacillus and a yeast strain of the genus Saccharomyces at a concentration of 1 × 10 ⁵ to 1 × 10 ⁶ cfu / ml, respectively. After culturing, it may be mixed and inoculated so that the total culture of each strain is 8 to 12% based on the total weight of bass and bluegill fish, preferably Bacillus genus strain, Lactobacillus genus strain and Saccharomyces genus yeast The strains may be inoculated and cultured at a concentration of 1.5×10 ⁵ cfu/ml, and then mixed and inoculated so that the total culture of each strain is 10% based on the total weight of the bass and bluegill fish, but is not limited thereto.

In the feed additive composition of the present invention, the strains of the genus Bacillus are Bacillus subtilis and Bacillus licheniformis , and the strains of the genus Lactobacillus are Lactobacillus plantarum , lactobacillus Bacillus casei ( Lactobacillus casei ), Lactobacillus acidophilus ( Lactobacillus acidophilus ), and the yeast of the genus Saccharomyces may be Saccharomyces cerevisiae , but is not limited thereto.

In addition, in the feed additive composition of the present invention, the amino acids are 20 naturally occurring amino acids, preferably proline, lysine, methionine, valine, serine, Cysteine, Arginine, Asparagine, Aspartic acid, Alanine, Isoleucine, Leucine, Threonine, Tyrosine, Glutamine ), glutamic acid, glycine, histidine, phenylalanine, or tryptophan, and most preferably proline, but is not limited thereto.

The feed additive composition of the present invention can improve the intestinal environment of livestock by increasing intestinal lactic acid bacteria and reducing intestinal E. coli, hydrogen sulfide and ammonia, and increasing the growth rate, feed efficiency and nutrient digestibility of fish, thereby increasing fish productivity can be improved.

The present invention also provides a feed composition for livestock or fish comprising the feed additive composition.

In the present invention, the term "feed" refers to any natural or artificial diet, one meal, etc., or a component of the one meal meal, for or suitable for eating, ingesting, and digesting by animals (livestock) or fish.

The feed composition of the present invention may be prepared in the form of conventional feed compositions such as powder and pellets, but is not limited thereto, and may be prepared in various types of feed known in the art.

The feed composition may include components commonly added to feed such as carbohydrates, proteins, fats, minerals, vitamins, minerals, and water. There is no particular restriction on the type of each of these components, and all commonly used ones in the art can be used.

The present invention also provides a fishing catch composition comprising a fermented product of bass and bluegill as an active ingredient.

In the fishing catch composition of the present invention, the fermented product of the bass and bluegill is as described above.

In the case of preparing the fishing catch composition of the present invention as a freshwater catch fish, the water content may be less than about 13%, and animal fish meal (pellet form) circulated in the surroundings may be separately added and used. In the case of manufacturing, the water content may be less than about 40%, and it may be prepared by mixing grains, by-products, and animal fish meal such as krill, but is not limited thereto.

Hereinafter, the present invention will be described in detail by examples. However, the following examples are only to illustrate the present invention, and the content of the present invention is not limited to the following examples.

Preparation Example 1. Preparation of bath and bluegill fermented products

In the present invention, the amino acid content was measured by preparing dry powders of fermented bath and bluegill in various ways. First, the fermenting bacteria are Bacillus subtilis ( Bacillus subtilis ), Bacillus licheniformis ( B. licheniformis ), Lactobacillus plantarum ( Lactobacillus plantarum ), Lactobacillus casei ( L. casei ), Lactobacillus acidophilus ( L. . _ All of the above strains were purchased and used from Biolix, and all strains were inoculated and cultured at a concentration of 1.5×10 ⁵ cfu/g, respectively.

Then, after mixing and crushing the bass and bluegill fish in equal amounts, mixing and inoculating so that the total culture of each strain is 10% (v / w) with respect to the total weight of the fish, and amino acid (proline) is 1% (w /w) to prepare bath and bluegill fermented products by culturing at 28-37 ° C for 48 hours, followed by 2-4 hours at 15 ° C, 3 hours at 25 ° C, 12 hours at 37 ° C, and 12 hours at 55 ° C. During low-temperature drying (Racelle dryer, DY-2230HR), when the moisture content fell below 13%, low-temperature dried powders of fermented bass and bluegill were prepared using pin wheat and corn mill mills.

In addition, the fermented bath and bluegill prepared in the same manner as above were hot-air dried at 55 ° C. for 24 hours and pulverized to prepare a hot-air dried powder of the fermented bath and bluegill, and freeze-dried the fermented bath and bluegill (Freeze Dryer, FDU-1200) to prepare a lyophilized powder of bath and bluegill fermentation.

As a result of measuring the amino acid content in the low-temperature dried powder, hot air-dried powder and freeze-dried powder of the fermented bath and bluegill prepared above (HF-21, Yamato Scientific Co., Ltd., Japan), the low-temperature dried powder of the fermented bath and bluegill Aspartic acid (Asp), Threonine (Threonine, Thr), Glutamic acid (Glu), Cysteine (Cys), Valine (Val), Methionine ( Methionine (Met), Isoleucine (Ile), Leucine (Leu), Tyrosine (Tyr), Phenylalanine (Phe), Lysine (Lys), and Histidine (His) It was confirmed that it contained more (Table 1). Through this, it was found that the low-temperature dried powder of the fermented bath and bluegill containing various amino acids can be usefully used as a raw material for feed additives.

Amino Acid Content (%) in Powder of Bass and Bluegill amino acid low temperature drying hot air dry freeze drying Asp 9.81 8.88 8.90 Thr 4.50 4.26 4.23 Glu 14.24 12.99 13.07 Cys 1.64 1.60 1.60 Val 4.62 4.01 4.06 Met 3.14 2.62 2.68 Ile 3.75 3.01 3.14 Leu 7.05 6.06 6.21 Tyr 4.06 3.19 3.28 Phe 4.13 3.34 3.40 Lys 8.07 6.92 7.10 His 2.27 1.86 1.86

Preparation Example 2. Preparation of feed containing bass and bluegill fermentation

As shown in Table 2 below, the low-temperature dried powder of fermented bass and bluegill was 50% by weight relative to the total 100% by weight of basic feed (control group, Di-Ref) to which the low-temperature dried powder of fermented bass and bluegill was not added. A feed (Di-BBFM-50) containing 30% by weight of low-temperature dried powder of fermented bass and bluegill based on 100% by weight of the total basal feed (Di-BBFM-30) was prepared.

feed composition Di-Ref Di-BBFM-50 Di-BBFM-30 Ingredients (%) Anchovy-Chile 63 19 37 Dehulled soybeans 5 5 5 wheat flour 18.3 17.8 18.3 blue gill,
largemouth bass fish meal - 50 30 Potato-starch 5 5 5 Soybean oil 5.5 - 1.5 Vitamin premix1 One One One Mineral premix2 One One One Vitamin C (50%) 0.3 0.3 0.3 Vitamin E (50%) 0.2 0.2 0.2 Choline (50%) 0.2 0.2 0.2 Cr ₂ O ₃ 0.5 0.5 0.5 Nutrient contents (dry matter basis) Dry matter (%) 92.9 93.0 95.2 Crude protein (%) 50.6 51.2 50.9 Crude lipid (%) 12.0 12.1 17.8 Ash (%) 12.4 10.8 10.0 NFE ³ (%) 25.0 25.9 21.3 Gross energy (kcal/g) 5.8 6.9 7.0

Example 1. Ornamental fish specification experiment using fermented bath and bluegill

The effect of feeding of feed containing low-temperature dried powder of bass and bluegill fermentation on ornamental fish was analyzed (Table 3).

Ornamental Fish Specification Experiment test species - ornamental fish Experiment period - A total of 56 days (8 weeks) experiment tank - 18 water tanks with a diameter of 1 m
- Breeding water per day circulation filtration
- 20 ornamental fish for each experimental tank experimental group - Ref (basic feed group, low-temperature dried powder of fermented bass and bluegill untreated)
- Di-BBFM-50 (feed feeding group containing 50% by weight of low-temperature dried powder of fermented bass and bluegill based on the total weight of basic feed)
- Di-BBFM-30 (Feeding group containing 30% by weight of low-temperature dried powder of fermented bass and bluegill based on the total weight of basic feed) breeding and instrumentation - All tanks are fed the same amount of feed twice a day
- At the time of final measurement, surviving objects are identified to measure the survival rate and the total weight of surviving objects

1-1. production analysis

As shown in Table 4 below, the growth rate (WG%), feed efficiency (FE%) and daily protein intake (DPI%) of ornamental fish were compared to the control group (basic feed), and the low-temperature drying of the fermented bass and bluegill of the present invention It was confirmed that the experimental group fed the feed containing powder (Di-BBFM-50) significantly increased.

1-2. Digestibility assay

The chromium oxide (Cr ₂ O ₃ ) method was used to measure the apparent digestibility coefficient (ADC) of feed, and was calculated by the method of CG Hutchins (Aquaculture, 1998, 161, 187-199). First, chromium oxide (indicator substance) was added to feed and fed to ornamental fish, and the amount of protein or chromium oxide in feed and feces was measured and calculated using the following formula.

ADC of dry matter (%)=(100−(dietary Cr ₂ O ₃ /feces Cr ₂ O ₃ )×100)

ADC of nutrients or energy (%) = {1-(dietary Cr ₂ O ₃ /feces Cr ₂ O ₃ )×(feces nutrient or energy/dietary nutrient or energy)}×100

ADC of test ingredient (%)=100/30×(ADC in test diet-0.7 ADC in reference diet).

As a result of measuring the nutrient digestibility of the feed, the experimental group (Di-BBFM-50) fed a feed containing 50% low-temperature dried powder of bass and bluegill fermentation in the basal feed, and the control group (Di-BBFM-50) fed only the basal feed -Ref) and the experimental group (Di-BBFM-30) fed a feed containing 30% low-temperature dried powder of bass and bluegill fermentation in the basic feed, it was confirmed that the digestibility of dry matter, protein, lipid and energy increased. (Table 5).

Measurement result of apparent digestibility coefficient Dry matter Protein Lipid Energy Di-Ref 81.6± ^0.2b 96.3± ^0.1b 97.2± ^0.2b 92.3± ^0.1b Di-BBFM-50 82.7± ^0.4c 98.1± ^0.2b 98.3± ^0.4a 94.9± ^0.2a Di-BBFM-30 78.9± ^0.0a 95.4± ^0.2a 97.6± ^0.1b 91.0± ^0.2a

Values (means±SE of three replications) in the same row not having a common superscript are significantly different ( P <0.05).

Example 2. Companion animal feeding experiment using fermented bath and bluegill

In order to analyze the effect of feeding feed containing low-temperature dried powder of bass and bluegill fermentation on companion animals, two small dogs (less than 4 kg), two medium-sized dogs (5-10 kg), and large dogs (more than 10 kg) ) 2 animals were fed a feed containing low-temperature dried powder of bass and bluegill fermentation twice a day. The experiment was conducted over about 14 weeks (22.04.21. ~ 22.07.25), and low-temperature dried powders of bass and bluegill fermentation were added at 3% by weight (w / w) relative to the total weight of the feed.

2-1. Analysis of E. coli and lactobacilli in feces

In order to check the number of microorganisms in feces, the feces on the first day (day 0, D-0 days) and 90 days (day D + 90) of feeding the pet with a feed containing low-temperature dried powder of bass and bluegill fermented animals. was collected and freeze-dried. After adding 1 g of fecal lyophilized powder to 10 ml of sterilized physiological saline, it was sufficiently suspended using a vortexer. After suspension, the mixture was diluted using the decimal dilution method, spread on Mcconkey medium or MRS medium (BD Difco ^TM , USA), and anaerobically cultured or normal cultured at 37°C. Colonies generated after culturing were measured with a measuring instrument to calculate the number of bacteria.

As a result of measuring the number of E. coli, small dog 1 decreased by 52.5% compared to the initial amount from 12,000 CFU/mL on D-0 day to 5,700 CFU/mL on D-90 day, and in the case of small dog 2, from 17,000 CFU/mL on D-0 day D-90 day 10,098 CFU / ㎖, about 40.6% compared to the initial decrease. In the case of medium-sized dog 1 and medium-sized dog 2, from 16,200 CFU/mL on D-0 day to 9,200 CFU/mL and 9,500 CFU/mL on D-90 day, respectively, a decrease of 43.2% and 41.4% compared to the initial period, respectively. In the case of large dog 1, from 27,000 CFU/mL on day D-0 to 23,000 CFU/mL on day D-90, a decrease of 14.8% from the initial level, and for large dog 2, from 28,000 CFU/mL on day D-0 to 18,000 CFU/mL on day D-90 ㎖ decreased by 35.7% compared to the initial period.

In addition, as a result of measuring the number of lactic acid bacteria, in the case of small dog 1, it increased by 77.5% from 1,670,000 CFU/ml on day D-0 to 2,965,000 CFU/ml on day D-90, and in the case of small dog 2, 130,000 CFU/ml on day D-0 In ㎖, D-90 day 134,200 CFU / ㎖ increased by about 3.2% compared to the initial. Medium dog 1 increased from 120,000 CFU/mL on day D-0 to 161,000 CFU/mL on day D-90, a 34.1% increase from the initial level, and for medium-sized dog 2, from 180,000 CFU/mL on day D-0 to 203,000 CFU/mL on day D-90. ㎖ increased by 12.8% compared to the initial period. Large dog 1 increased from 175,000 CFU/mL on day D-0 to 229,000 CFU/mL on day D-90, a 30.9% increase from the initial level, and for large dog 2, from 12,000 CFU/mL on day D-0 to 19,200 CFU/mL on day D-90 ㎖ increased by 60% compared to the initial period. Through this, it was found that the fermented bath and bluegill of the present invention can reduce harmful bacteria in the intestines of companion animals and increase beneficial bacteria in the intestines.

2-2. Analysis of hydrogen sulfide and ammonia in feces

In order to measure hydrogen sulfide in feces of companion animals, it was measured using GV-100S (Gastec, Korea) equipped with a hydrogen sulfide detection tube at a height of about 1 cm from the entrance of the tube containing feces. In addition, the indophenol method was used to measure ammonia in feces. After diluting the supernatant obtained by centrifugation of the suspended feces by 2 times with saline, 2.5 ml of the diluted sample was mixed with 0.1 ml of phenol solution, 0.1 ml of nitroprusside sodium solution and 0.25 ml of oxidizing solution, and incubated at room temperature. After reacting for 1 hour, the absorbance was measured using a spectrophotometer (Spectrophotometer, Biotek, Korea) after the reaction was over.

As a result of measuring hydrogen sulfide, in the case of small dog 1, it decreased by 20% from 2.0 ppm on day D-0 to 1.6 ppm on day D-90, and in the case of small dog 2, from 1.3 ppm on day D-0 to 1.0 ppm on day D-90. It decreased by 23.1% compared to the previous year. In the case of medium-sized dog 1, it decreased by 15.4% from 1.3 ppm on day D-0 to 1.1 ppm on day D-90, and in the case of medium-sized dog 2, from 0.2 ppm on day D-0 to 0.1 ppm on day D-90, a decrease of 50% compared to the initial period. . In the case of large dog 1, it decreased by 25% from 0.4 ppm on day D-0 to 0.3 ppm on day D-90, and in the case of large dog 2, from 0.4 ppm on day D-0 to 0.2 ppm on day D-90, a decrease of about 50% .

In addition, as a result of measuring ammonia, in the case of small dog 1, it decreased by 16% from 0.56 ppm on day D-0 to 0.47 ppm on day D-90, and in case of small dog 2, it decreased from 1.33 ppm on day D-0 to 1.07 on day D-90. In ppm, it decreased by 19.6% compared to the initial period. Medium-sized dog 1 decreased by 46.4% from 0.97 ppm on day D-0 to 0.52 ppm on day D-90, and in case of medium-sized dog 2, decreased by 38.1% from 0.84 ppm on day D-0 to 0.52 ppm on day D-90. did In the case of large dog 1, it decreased by 14.3% from 1.4 ppm on day D-0 to 1.2 ppm on day D-90, and in the case of large dog 2, it decreased by 18.8% from 0.64 ppm on day D-0 to 0.52 ppm on day D-90. Through this, it was found that the fermented bath and bluegill of the present invention is effective in improving bowel movements such as enteritis or constipation by improving the intestinal environment of companion animals.

Claims (8)

A feed additive composition for companion animals or ornamental fish containing fermented products of bass and bluegill as an active ingredient,
The fermented product of the bass and bluegill,
(1) After mixing and crushing bass and bluegill fish, Bacillus subtilis , Bacillus licheniformis , Lactobacillus plantarum, Lactobacillus casei Inoculating a mixture of Lactobacillus acidophilus and Saccharomyces cerevisiae strains, and adding proline to ferment; and
(2) The fermented product of the bass and bluegill is 2 to 4 hours at 13 ~ 17 ℃, 2 ~ 4 hours at 23 ~ 27 ℃, 11 ~ 13 hours at 35 ~ 39 ℃ and 11 ~ 13 hours at 53 ~ 57 ℃ Drying step by step during; Companion animal or ornamental fish feed additive composition, characterized in that produced through. delete delete The feed additive composition for companion animals or ornamental fish according to claim 1, wherein the strain mixture is inoculated at 8 to 12% based on the total weight of the bass and bluegill fish. delete The method of claim 1, wherein the feed additive composition has an effect of increasing lactic acid bacteria in the intestines of companion animals and reducing intestinal coliforms, hydrogen sulfide and ammonia, and has an effect of increasing growth rate, feed efficiency and nutrient digestibility of ornamental fish. Feed additive composition for animals or ornamental fish. A feed composition for companion animals or ornamental fish comprising the feed additive composition according to any one of claims 1, 4 and 6. A fishing catch composition comprising fermented product of bass and bluegill as an active ingredient,
The fermented product of the bass and bluegill,
(1) After mixing and crushing bass and bluegill fish, Bacillus subtilis , Bacillus licheniformis , Lactobacillus plantarum, Lactobacillus casei Inoculating a mixture of Lactobacillus acidophilus and Saccharomyces cerevisiae strains, and adding proline to ferment; and
(2) The fermented product of the bass and bluegill is 2 to 4 hours at 13 ~ 17 ℃, 2 ~ 4 hours at 23 ~ 27 ℃, 11 ~ 13 hours at 35 ~ 39 ℃ and 11 ~ 13 hours at 53 ~ 57 ℃ Drying sequentially during the fishing catch composition, characterized in that produced through.