KR102549206B1 - Detecting apparatus for dna or rna, kit comprising same, and sensing method for dna or rna - Google Patents

Abstract

Board; one or more chambers formed on a surface of the substrate; a plurality of nanostructures fixed to an inner surface of the one or more chambers; one or more light sources providing incident light to the one or more chambers; And it provides a detection device for detecting at least one of DNA and RNA including a light receiving unit for detecting a light signal emitted from the nanostructure.

Description

DNA or RNA detection device, kit including the detection device, and DNA or RNA detection method

It relates to a DNA or RNA detection device, a kit including the detection device, and a DNA or RNA detection method.

To elute intracellular DNA, mechanical force using a vortex mixer, or a method of treating chemicals such as lysozyme, EDTA (Ethylene Diamine Tetr-Acetate), SDS (Sodium dodecyl sulfate) is used. .

DNA extracted from cells is amplified by polymerase chain reaction (PCR) and used for analysis. It is a key skill.

A typical polymerase chain reaction is to separate (denaturate) a DNA double-strand into a single-strand at about 95 ° C, lower the temperature, and a primer having a base sequence complementary to the substrate of the DNA single-strand binds (Annealing), By raising the temperature again, DNA polymerase polymerizes dNTPs complementary to the DNA single strand from the 3'-OH end of the primer bound to the DNA single strand to synthesize a DNA double helix. The DNA repeats the separation-binding-polymerization cycle a certain number of times, and the synthesized DNA double helix is used as a substrate for the next cycle to amplify the DNA.

Recently, amplified DNA is quantified by a real-time PCR method that can quantify the amplified DNA in each cycle in real time by adding a fluorescent substance as a marker during the polymerase chain reaction, and real-time PCR is performed according to the experimental method. It can be measured by directly measuring the amount of amplified DNA or by using a Taqman method that quantifies the fluorescence generated by using a dye that binds to DNA and probe DNA.

In order to perform a series of processes of extracting and amplifying DNA by lysing the cells, various physical and chemical means, complex and precise large equipment such as a thermo-cycler are required, and quantitative measurement of the amplified DNA is required. In order to do this, more independent detection means are needed.

One embodiment provides a device for detecting at least one of DNA and RNA capable of isolating, amplifying, and detecting at least one of DNA and RNA on a single substrate.

Another embodiment provides a kit comprising the device.

Another embodiment provides a method for detecting at least one of DNA and RNA using the above device.

One embodiment is a substrate; one or more chambers formed over the substrate; a plurality of nanostructures fixed to an inner surface of the one or more chambers; one or more light sources providing incident light to the one or more chambers; And it provides a detection device for detecting at least one of DNA and RNA including a light receiving unit for detecting a light signal emitted from the nanostructure.

The one or more chambers may include one or more first heating chambers and one or more detection chambers.

The one or more chambers further include one or more sample chambers, one or more fluid channels connecting between the one or more sample chambers and the one or more first heating chambers, the one or more first heating chambers and the one or more detection chambers. It may further include one or more fluid channels connecting therebetween.

The one or more chambers may further include one or more reagent chambers, and may further include one or more fluid channels connecting between the one or more reagent chambers and the one or more first heating chambers.

The substrate may be a transparent glass or polymer having a refractive index of 1.3 to 1.9.

The glass includes any one selected from SiO ₂ , BK7, SF10, SF11, N-LASF46A, and combinations thereof, and the polymer is a polystyrene-based polymer, a polymethylmethacrylate polymer, a polycarbonate-based polymer, and a cyclic olefin copolymers, or combinations thereof.

The nanostructure may be a spherical metal nanoparticle, a core-shell nanoparticle having a dielectric core and a metal shell, or a combination thereof.

In the spherical metal nanoparticles, the metal may be gold (Au), silver (Ag), copper (Cu), aluminum (Al), or a combination thereof.

In the core-shell nanoparticles in which the core is a dielectric and the shell is a metal, the dielectric is SiO ₂ , BK7, SF10, SF11, N-LASF46A, a polystyrene-based polymer, a polymethylmethacrylate polymer, a polycarbonate-based polymer, A cyclic olefin copolymer, or a combination thereof, and the metal may include gold (Au), silver (Ag), copper (Cu), aluminum (Al), or a combination thereof.

The nanostructure may have a size of 1 nm to 1000 nm.

An inner surface of the one or more first heating chambers may be coated with a reflection coating.

The one or more detection chambers may include one or more thermal coolers.

At least one of the one or more light sources may emit light having a wavelength ranging from 10 nm to 10 μm.

The at least one light source may independently induce a photothermal phenomenon of the nanostructure to increase the temperature of the surrounding medium, light having a wavelength that increases the temperature of the surrounding medium, plasmon resonance absorption of the nanostructure, maximization of scattering efficiency, or both. Light having , or light having a wavelength that induces a photothermal phenomenon of the nanostructure to increase the temperature of the surrounding medium while absorbing plasmon resonance of the nanostructure, maximizing scattering efficiency, or performing both may be irradiated.

The at least one light source may independently emit monochromatic light or multi-color light.

One or more polarizers, color filters, or a combination thereof may be further included between the substrate and the one or more light sources.

One or more light receivers may be present on the same surface of the substrate as the one or more light sources, or may be located on a surface opposite to the one or more light sources with respect to the substrate.

The detection device for one or more of the DNA and RNA may further include a control unit for adjusting the wavelength of light incident to the one or more chambers and the on/off cycle of the one or more light sources.

In another embodiment, a kit including a reagent reacting with a sample containing at least one of DNA and RNA, and a detection device for at least one of DNA and RNA according to one embodiment is provided.

In another embodiment, a sample containing at least one of DNA and RNA to be analyzed, a polymerase, and a base are placed in one or more chambers formed on a substrate and having a plurality of nanostructures fixed to an inner surface thereof; controlling the temperature of the sample by irradiating light into the chamber, thereby causing a polymerization reaction to amplify at least one of DNA and RNA including a specific sequence in at least one of the DNA and RNA; Provided is a method for detecting at least one of DNA and RNA containing a specific sequence, comprising irradiating light to a chamber in which the polymerization reaction is completed and detecting an absorbed or scattered light signal.

A device according to an embodiment separates at least one of DNA and RNA, amplifies, and Provided is an all-optical detection technology for one or more of DNA and RNA capable of performing detection (eg, quantitative detection) on a single substrate.

1 is a plan view schematically illustrating a substrate of a device for detecting at least one of DNA and RNA according to an embodiment of the present invention.
2 is a cross-sectional view of a spherical metal nanoparticle according to an embodiment of the present invention.
3 is a plan view schematically showing that one or more spherical metal nanoparticles are fixed to an inner surface of a chamber of a device according to an embodiment of the present invention.
4 is a cross-sectional view of a core-shell type nanoparticle according to an embodiment of the present invention.
5 is a plan view schematically showing that one or more core-shell nanoparticles are fixed to the inner surface of a chamber of a device according to an embodiment of the present invention.
6 is a plan view schematically illustrating a detection device in which a plurality of chambers are formed on one substrate according to an embodiment of the present invention.
7 is a plan view schematically illustrating a detection device in which a plurality of chambers and one or more fluid channels are formed on one substrate according to an embodiment of the present invention.
FIG. 8 is a cross-sectional view of the substrate of FIG. 7 taken along line A-A′, in which a light source not shown in FIG. 7 and a light receiving unit positioned on the same plane as the light source are added to the detection device according to an embodiment. This is a schematic cross-sectional view.
FIG. 9 is a cross-sectional view of the substrate of FIG. 7 taken along line A-A′, showing a light source not shown in FIG. 7 and a light receiving unit positioned on a plane facing the light source with the substrate interposed therebetween. It is a schematic cross-sectional side view of the detection device according to the embodiment.
10 is a graph showing plasmon resonance absorption efficiency (σ _abs ) of spherical gold nanoparticles in water, which varies depending on the size of the radius of the spherical gold nanoparticles.
11 is a graph showing the temperature change (ΔT) of water according to plasmon resonance absorption of spherical gold nanoparticles in water, which varies according to the size of the radius of the spherical gold nanoparticles.
12 shows the plasmon resonance absorption efficiency (σ _abs ) is the result of theoretical calculation.
13 shows plasmon resonance absorption in water of core-shell nanoparticles in which the core is silica with a radius of 15 nm (a) and the shell is gold (Au), and the core-shell nanoparticles vary depending on the thickness of the shell. It shows the result of theoretically calculating the efficiency (σ _abs ).
FIG. 14 schematically illustrates a method of changing the temperature by controlling the operating time of a light source and a thermal cooler according to time in order to amplify and quantitatively detect at least one of DNA and RNA in at least one chamber.

Advantages and features of the present invention, and methods for achieving them, will become clear with reference to the embodiments described below in detail in conjunction with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below and may be implemented in various different forms, but only the present embodiments make the disclosure of the present invention complete, and the common knowledge in the art to which the present invention belongs It is provided to fully inform the holder of the scope of the invention, and the present invention is only defined by the scope of the claims. Like reference numbers designate like elements throughout the specification.

Terms used in this specification are for describing embodiments and are not intended to limit the present invention. In this specification, singular forms also include plural forms unless specifically stated otherwise in a phrase. As used herein, 'comprises' and/or 'comprising' does not preclude the presence or addition of one or more other elements or steps to a stated element or step.

In addition, the embodiments described in this specification will be described with reference to cross-sectional views, side cross-sectional views, and/or plan views, which are ideal exemplary views of the present invention. In the drawings, the thickness of regions is exaggerated for effective description of technical content. Accordingly, the shape of the illustrative drawings may be modified due to manufacturing techniques and/or tolerances. Therefore, embodiments of the present invention are not limited to the specific shapes shown, but also include changes in shapes generated according to manufacturing processes. For example, an etched region shown at right angles may have a predetermined curvature. Accordingly, the regions illustrated in the drawings have schematic properties, and the shapes of the regions illustrated in the drawings are intended to illustrate a specific shape of a region of a device and are not intended to limit the scope of the invention.

When a part such as a plate, substrate, chamber, cooler, layer, film, region, etc. is said to be "on" another part, this includes not only the case where it is "directly on" the other part, but also the case where there is another part in between. . Conversely, when a part is said to be "directly on" another part, it means that there is no other part in between.

A device according to an embodiment includes one substrate, one or more chambers formed on the substrate, and a plurality of nanostructures fixed to an inner surface of the chamber. The device according to the embodiment can be used to detect and/or quantify various samples, eg, amplify and detect DNA and/or RNA.

Specifically, the detection device according to an embodiment of the present invention determines the size and material of the nanostructure fixed on the inner surface of the chamber, the wavelength of light entering the chamber, or the On/Off cycle of the light source for irradiating light into the chamber. By controlling the photothermal phenomenon and/or plasmon resonance of the nanostructure, amplification and detection of DNA and/or RNA can be performed all-optically on a single substrate. In one embodiment, the device is capable of amplifying and detecting DNA and/or RNA within one or more chambers, as well as eluting DNA and/or RNA from cells. Furthermore, the detection may be a quantitative detection.

A DNA and/or RNA detection device according to one embodiment of the present invention, a kit including the detection device, and a method for detecting at least one of DNA and RNA using the detection device will be described with drawings.

1 is a plan view schematically illustrating a substrate of a device according to an embodiment of the present invention.

Referring to FIG. 1 , a device according to an embodiment includes a substrate 100 and includes one or more chambers formed over the substrate 100 . 1 shows a device in which only one chamber 110 is formed on one substrate 100 according to an embodiment, but a plurality of chambers may be formed on one substrate 100 .

As shown in FIG. 1, the detection device according to one embodiment may include only one chamber 110, and on the inner surface of one chamber 110, in the form described later in FIGS. 2 to 5. , a plurality of nanostructures 200 can be immobilized. In this way, a sample to be analyzed, for example, DNA and/or RNA, is placed in a chamber in which a plurality of nanostructures 200 are fixed, and light is irradiated into the chamber from a light source (not shown). At this time, the nanostructure 200 fixed to the inner surface of the chamber by the light causes a photothermal reaction by surface plasmon resonance, and the temperature of the medium around the nanostructure 200 may be increased. When the temperature rises in this way, the DNA and / or RNA in the sample is separated into single strands, and after putting primers, polymerases, dNTPs, etc. for DNA and / or RNA polymerization therein, one chamber (110 ) By changing the temperature by adjusting the wavelength of light incident on the light source, the On / Off cycle of the light source, and the On / Off cycle of the thermal cooler, the polymerization reaction of the DNA and / or RNA may occur. In this way, DNA and/or RNA having a specific sequence is amplified by repeating the separation and polymerization reaction of DNA and/or RNA, and a wavelength equal to or different from the light is emitted from a light source (not shown) into one chamber 110. DNA having a specific sequence and / or RNA can be detected. The detection may be performed in a light receiving unit (not shown), and the detection may be quantitative detection.

The substrate 100 may be a flat plate. The substrate 100 may be a transparent glass or polymer having a high refractive index of 1.3 to 1.9, 1.4 to 1.8, or 1.4 to 1.7. The glass may be a hard glass, and the polymer may be a bendable or deformable polymer. The glass is selected from silicate glass (SiO ₂ ), borosilicate glass, BK7 (manufactured by Schott AG), SF10 (manufactured by Schott AG), SF11 (manufactured by Schott AG), N-LASF46A (manufactured by Schott AG), and combinations thereof It may include any one, and the polymer having a high refractive index may include a polystyrene-based polymer, a polymethyl methacrylate polymer, a polycarbonate-based polymer, a cyclic olefin copolymer, or a combination thereof.

The one or more chambers may contain samples, reagents, or combinations thereof.

The sample may be a sample containing one or more of DNA and RNA, and the sample containing one or more of DNA and RNA may include cells, cell suspensions, cell nuclei, extracted DNA or extracted RNA, , The extracted DNA or extracted RNA can be any one that can be amplified by the subsequent polymerase chain reaction method, such as total cell DNA, plasmid DNA, phage DNA ( phage DNA), cDNA, or mRNA.

The reagent is, for example, a buffer capable of suspending cells (suspension buffer), a buffer that helps cell lysis (cell lysis buffer), primers necessary for amplification of DNA and / or RNA by polymerase chain reaction, polymerization It may be a polymerase, deoxyribonucleotide triphosphate (dNTP), magnesium chloride (MgCl ₂ ), a phosphor that can be used as a marker for quantitative detection of amplified DNA and/or RNA, or a combination of two or more thereof. The phosphor may include an organic fluorophore, a fluorescent protein, or a quantum dot.

At least one of the one or more chambers may include a plurality of nanostructures 200 fixed to an inner surface, and the nanostructures 200 may have clear boundaries and independent shapes with respect to a surrounding medium. there is.

As described in detail later, the plurality of nanostructures 200 may be a nanoheater that absorbs light incident from the light source and exhibits a photothermal phenomenon, a nanoantenna that exhibits plasmon resonance absorption and scattering, or a combination thereof. can Cells of the sample may be thermally lysed by heat generated by a photothermal phenomenon in the nanoheater, and DNA or RNA may be amplified by a polymerase chain reaction of DNA or RNA. DNA or RNA can be quantitatively detected by plasmon resonance absorption and scattering of incident light incident on the nanoantenna.

The plurality of nanostructures 200 fixed to the surface of the one or more chambers may independently act as nanoheaters or nanoantennas, and one nanostructure 200 may simultaneously function as a nanoheater and nanoantenna.

2 to 5 show a nanostructure 200 fixed to the inner surface of one or more chambers in the DNA and/or RNA detection device according to the present invention.

FIG. 2 is a cross-sectional view of a spherical metal nanoparticle 230 according to an exemplary embodiment, and a' is a radial length of the spherical metal nanoparticle 230 .

FIG. 3 is a plan view schematically illustrating a form in which one or more spherical metal nanoparticles 230 are fixed to an inner surface of one or more chambers formed on a substrate 100 in the detection device.

4 is a cross-sectional view of a core-shell nanoparticle 240 according to an embodiment, a is the radial length of the core in the core-shell nanoparticle 240, and b is the core-shell nanoparticle 240 is the radius length of the entire nanoparticle including core and shell in .

5 is a plan view schematically showing a form in which one or more core-shell nanoparticles 240 are fixed to the inner surface of one or more chambers formed on the substrate 100 in the detection device.

As shown in FIGS. 2 and 3, the nanostructure 200 is a spherical metal nanoparticle 230 or, as shown in FIGS. 4 and 5, a core-shell nanoparticle having a dielectric core and a metal shell. particle 240, or a combination thereof.

The plurality of spherical metal nanoparticles 230 fixed to the surface of the one or more chambers may have excellent plasmon resonance absorption efficiency (σ _abs ) of the irradiated light source. The plurality of core-shell nanoparticles 240 fixed to the surface of the one or more chambers may have a wide range of adjustable wavelengths. Therefore, those skilled in the art can implement a nanoantenna that can easily detect DNA and/or RNA by selecting the shape and combination of nanostructures as needed.

A photothermal phenomenon of the nanostructure, a plasmon resonance absorption efficiency (σ _abs ), a heating capacity (Q _abs ), and a description related to a temperature change (ΔT) of a surrounding medium changed by the nanostructure will be described later.

In the spherical metal nanoparticles 230, the metal may have a distinct plasmon resonance effect in a wide wavelength range. In the spherical metal nanoparticles, the metal may be gold (Au), silver (Ag), copper (Cu), aluminum (Al), or a combination thereof.

When the metal is used, the plasmon resonance absorption efficiency (σ _abs ) of the spherical metal nanoparticles is high in the near-infrared wavelength region where the heat absorption cross section of the body fluid is large, and cell dissolution by heat (thermal lysis) and DNA or RNA isolation can be facilitated. In addition, plasmon resonance absorption and/or scattering efficiency are maximized in the visible ray region, and DNA and/or RNA can be easily quantitatively detected.

When a fluorescent substance is used as a marker for quantitative detection of amplified DNA and/or RNA, a conventional fluorescent substance having a wide absorption wavelength in the ultraviolet to near infrared region can be used as the marker.

In the core-shell nanoparticle 240 in which the core is a dielectric and the shell is a metal, the dielectric is SiO ₂ , BK7 (manufactured by Schott AG), SF10 (manufactured by Schott AG), SF11 (manufactured by Schott AG), N- LASF46A (manufactured by Schott AG), a polystyrene-based polymer, a polymethyl methacrylate polymer, a polycarbonate-based polymer, a cyclic olefin copolymer, or a combination thereof, wherein the metal is gold (Au), silver (Ag), It may include copper (Cu), aluminum (Al), or a combination thereof. The core may be a dielectric made of the same material as or different from that of the substrate 100 .

As described above, the plurality of spherical metal nanoparticles 200 fixed to the surface of one or more chambers may have excellent plasmon resonance absorption efficiency (σ _abs ) of the irradiated light source, and may be fixed to the surface of the one or more chambers. The plurality of core-shell nanoparticles 240 may have a wide range of controllable wavelengths, and those skilled in the art can select a combination of the shape of the nanostructure and the material (eg, the aforementioned dielectric and/or metal) as needed. Thus, it is possible to realize a nanoantenna for easy quantitative detection of DNA and/or RNA.

The nanostructure 200 may have a size of 1 nm to 1000 nm, 5 nm to 500 nm, or 10 nm to 100 nm. When the nanostructure is the spherical metal nanoparticle 230, the size of the nanostructure means the particle diameter of the spherical nanoparticle. When the nanostructure is the core-shell nanoparticle 240, the size of the nanostructure means the particle diameter of the entire nanoparticle including the core and the shell. As will be described later, when the nanostructure 200 has a size in the above range, the nanostructure 200 may have an excellent photothermal effect.

Although not shown in FIG. 1 , inner surfaces of the one or more chambers may be coated with a reflection coating. The reflective coated chamber may reduce loss of a light source by reflecting incident light.

Although not shown in FIG. 1 , the one or more chambers may include one or more thermal coolers. The thermal cooler may be located below the one or more chambers. The temperature of the chamber is controlled by adjusting the On / Off cycle of the thermal cooler, and the DNA or RNA is amplified by controlling the thermal lysis of the sample and the polymerase chain reaction cycle of DNA or RNA. there is.

Although not shown in FIG. 1 , at least one of the one or more light sources may emit light having a wavelength ranging from 10 nm to 10 μm. At least one light source among the one or more light sources may emit light having a different wavelength from the other light sources.

The one or more light sources, each independently inducing a photothermal phenomenon of the nanostructure 200 to increase the temperature of the surrounding medium, light having a wavelength (hv ₁ ), plasmon resonance absorption of the nanostructure 200, maximization of scattering efficiency , Or light having a wavelength (hv ₂ ) that performs both, or plasmon resonance absorption of the nanostructure while increasing the temperature of the surrounding medium by inducing photothermal phenomena of the nanostructure, maximizing scattering efficiency, or both Light having both wavelengths (hv ₁ and hv ₂ ) may be irradiated.

The wavelength (hv ₁ ), which induces the photothermal phenomenon of the nanostructure 200 and raises the temperature of the surrounding medium, is the wavelength at which plasmon resonance absorption is maximized to facilitate thermal dissolution of cells and separation of DNA and/or RNA. Considering the wavelength, those skilled in the art can select it as needed.

When a phosphor is used as a marker for quantitative detection of amplified DNA and/or RNA, the wavelength (hv ₂ ) at which the nanostructure absorbs plasmon resonance, maximizes the scattering efficiency, or performs both is the maximum absorption of the phosphor. A person skilled in the art can select according to need in consideration of the wavelength to be.

The one or more light sources may independently radiate light to the one or more chambers.

The one or more light sources may independently emit monochromatic light or polychromatic light, and preferably monochromatic light. For example, the monochromatic light may be laser light, and may be, for example, a gas laser, a solid-state laser, a semiconductor laser, or a laser diode. The multicolored light may be light emitted from a Xenon lamp, a white LED, a light emitting diode, a halogen lamp, an infrared light source, or the like.

When a phosphor is used as a marker for detecting amplified DNA and/or RNA, the monochromatic light may be light having a wavelength at which the phosphor absorbs the maximum, and the polychromatic light includes a wavelength at which the phosphor absorbs the maximum. It may be light having a range.

Although not shown in FIG. 1 , the device according to the embodiment may further include one or more polarizers, color filters, or a combination thereof between the substrate 100 and the one or more light sources. The polarizer may control polarization of light incident on the substrate 100 . The color filter may select a wavelength of light incident on the substrate 100 .

Although not shown in FIG. 1, the light receiving unit is located on the same surface as the one or more light sources with respect to the substrate 100, or is located on the surface opposite to the one or more light sources with respect to the substrate 100, and has one or more light sources. can exist

When a fluorescent substance is used as a marker for quantitative detection of amplified DNA and/or RNA, the light receiving unit may include one or more photodetectors capable of detecting a fluorescence signal of the fluorescent substance.

Although not shown in FIG. 1, the light receiving unit includes an optical detector capable of measuring an optical signal emitted or scattered by plasmon resonance absorption and/or scattering of the nanostructure 200 fixed to the surface of the one or more chambers. and, when a fluorescent substance is used as a marker for detection of amplified DNA and/or RNA, an optical detector capable of measuring a fluorescent signal of the fluorescent substance may be included.

Although not shown in FIG. 1 , the device according to the embodiment may further include one or more polarizers, color filters, or a combination thereof between the substrate 100 and the light receiving unit. The polarizer may control the polarization of light reflected, transmitted, emitted, or scattered from the substrate 100 . The color filter may select a wavelength of light reflected, transmitted, emitted, or scattered from the plurality of nanostructures 200, and preferably may select a wavelength of light reflected, transmitted, emitted, or scattered from the plurality of nanoantennas. there is. When a phosphor is used as a marker, the color filter may be a color filter capable of selecting a wavelength of a fluorescence signal of the phosphor.

Although not shown in FIG. 1, the detection device according to the embodiment may further include a control unit for adjusting the wavelength of light incident to the one or more chambers and the on/off cycle of the one or more light sources. . The detection device of at least one of the DNA and RNA may further include a control unit for controlling an on/off cycle of the heat cooler. The control unit adjusts the wavelength of light incident on the one or more chambers described above, the on / off cycle of the one or more light sources, and / or the on / off cycle of the heat cooler, DNA and / or RNA can be amplified by controlling the thermal lysis of DNA and / or RNA polymerase chain reaction cycle.

6 is a plan view schematically illustrating an apparatus in which a plurality of chambers are formed on one substrate according to another embodiment of the present invention.

In the detection device according to the embodiment shown in FIG. 6 , the substrate 100 is the same as that described in FIG. 1 .

Referring to FIG. 6 , in the detection device according to an exemplary embodiment, the one or more chambers may include one or more first heating chambers 111 and one or more detection chambers 112 .

One or more first heating chambers 111 may hold samples, reagents, or a combination thereof, and the samples and reagents are as described above.

The one or more first heating chambers 111 may include a plurality of nanostructures 210 fixed to an inner surface, and the nanostructures 210 may include a plurality of nanostructures 210 fixed to the inner surface of the at least one chamber of FIG. 1 . As described above in the description of the structure 200 and the description of FIGS. 2 to 5 .

The plurality of nanostructures 210 fixed to the inner surface of one or more first heating chambers 111 may preferably be the aforementioned nanoheaters. Cells of the sample may be thermally lysed by heat generated by a photothermal phenomenon from the plurality of nanoheaters fixed to the inner surface of the one or more first heating chambers 111 .

The plurality of nanostructures 210 fixed to the inner surface of the one or more first heating chambers 111 may be, for example, the aforementioned core-shell nanoparticles 240 having a dielectric core and a metal shell. .

Although not shown in the drawings, inner surfaces of one or more first heating chambers 111 may be coated with reflection. The reflective coated first heating chamber 111 may reduce light loss by reflecting incident light.

In one or more detection chambers 112, DNA and/or RNA may be amplified by polymerase chain reaction, and the amplified DNA and/or RNA may be detected. The one or more detection chambers 112 may include a plurality of nanostructures 220 fixed to an inner surface, and the nanostructures may include a plurality of nanostructures 200 fixed to the inner surface of the at least one chamber in FIG. 1 . As described above in the description and the description of FIGS. 2 to 5 .

One or more detection chambers 112 may include a plurality of nanostructures 220 anchored to an inner surface. For example, the plurality of nanostructures 220 fixed to the inner surface of one or more detection chambers 112 may be the nano-heater, the nano-antenna, a nano-structure capable of serving as both the nano-heater and the nano-antenna at the same time, or It may be a combination of these.

By periodically controlling the temperature change caused by the photothermal phenomenon of the plurality of nanoheaters fixed on the inner surface of the one or more detection chambers 112, the DNA and/or RNA polymerase chain reaction occurs in the one or more detection chambers 112. DNA and/or RNA may be amplified. The amplified DNA and/or RNA may be detected using plasmon resonance absorption and scattering of light incident to the plurality of nanoantennas fixed to the inner surface of the one or more detection chambers 112 . The detection may be quantitative detection.

The plurality of nanostructures 220 fixed to the surface of the one or more detection chambers 112 may each independently act as a nanoheater or nanoantenna, and one nanostructure may act as both a nanoheater and a nanoantenna at the same time. .

The plurality of nanostructures 220 fixed to the surface of the one or more detection chambers 112 may be, for example, the aforementioned core-shell nanoparticles 240 having a dielectric core and a metal shell.

One or more detection chambers 112 may include one or more thermal coolers 141 . The heat cooler 141 is as described above.

Although not shown in the drawings, a device according to an embodiment may include one or more light sources, and the one or more light sources are as described above.

A light source for irradiating light having a wavelength (hv ₁ ) that induces the photothermal phenomenon of the nanostructure and increases the temperature of the surrounding medium is one or more first heating chambers 111 and/or one or more detection chambers 112 light can be irradiated.

A light source emitting light having a wavelength (hv ₂ ) that absorbs the plasmon resonance of the nanostructure, maximizes scattering efficiency, or both of these can radiate light to one or more detection chambers 112.

A light source irradiating light having a wavelength that performs plasmon resonance absorption of the nanostructure, maximization of scattering efficiency, or both while increasing the temperature of the surrounding medium by inducing the photothermal phenomenon of the nanostructure described above is one or more first light sources. Light may be irradiated to the heating chamber 111 and/or one or more detection chambers 112 .

Although not shown in FIG. 6 , the detection device according to the embodiment may further include the aforementioned polarizer, color filter, light receiving unit, and adjusting unit.

7 is a plan view schematically illustrating a device in which a plurality of chambers and one or more fluid channels are formed on one substrate according to another embodiment of the present invention.

In the device according to an embodiment shown in FIG. 7 , the substrate 100 , the one or more first heating chambers 111 , the one or more detection chambers 112 , the one or more first heating chambers 111 are secured to the inner surface. The plurality of nanostructures 210 fixed to each other, the plurality of nanostructures 220 fixed to the inner surface of one or more detection chambers 112, and the thermal cooler 141 are as described in FIGS. 1 to 6 .

Referring to FIG. 7 , in the device according to an exemplary embodiment, the one or more chambers further include one or more sample chambers 113, and between the one or more sample chambers 113 and the one or more first heating chambers 111 It may further include one or more fluid channels 160 connecting the , and one or more fluid channels 160 connecting between the one or more first heating chambers 111 and the one or more detection chambers 112 . Each of the one or more fluid channels 160 may independently include one or more filters.

One or more sample chambers 113 may contain the aforementioned sample or a mixture of the aforementioned sample and the aforementioned reagent. The foregoing sample, or a mixture of the foregoing sample and the foregoing reagent, is transported from the one or more sample chambers 113 through the fluid channel 160 connecting the one or more sample chambers 113 and the one or more first heating chambers 111. It may be transferred to one or more first heating chambers 111 . A sample thermally melted in the first heating chamber 111 or a mixture of the sample and the aforementioned reagent is transferred from the one or more first heating chambers 111 to one or more first heating chambers 111 and one or more detection chambers 112. It can be transported to one or more detection chambers 112 through the fluid channel 160 connecting the .

The one or more chambers may further include one or more reagent chambers 114, and one or more fluid channels 160 connecting between the one or more reagent chambers 114 and the one or more first heating chambers 111 may be further included. . Each of the one or more fluid channels 160 may independently include one or more filters.

One or more reagent chambers 114 may contain the aforementioned reagents or a mixture of the aforementioned samples and the aforementioned reagents. The foregoing reagents, or mixtures of the foregoing samples and the foregoing reagents are transported from one or more reagent chambers (114) through a fluid channel (160) connecting one or more reagent chambers (114) and one or more first heating chambers (111). It may be transferred to one or more first heating chambers 111 .

Although not shown in FIG. 7 , the one or more chambers may further include one or more waste chambers, and may further include one or more fluid channels connecting the one or more waste chambers and the one or more detection chambers 112 . . Each of the one or more fluid channels may independently include one or more filters. The one or more waste chambers include materials to be removed in the process of amplifying DNA and/or RNA in the one or more detection chambers 112, materials to be removed in the process of detecting the amplified DNA and/or RNA, wastes, or materials thereof. combination can be supported.

Although not shown in FIG. 7, the detection device shown in FIG. 7 may further include at least one of the one or more light sources, polarizers, color filters, light receiving units, and control units described in FIGS. 1 and 6, and each of these components As described above.

FIG. 8 is a cross-sectional view of the substrate of FIG. 7 taken along line A-A′, and includes light sources 170 and 180 not shown in FIG. 7 and a light receiving unit 190 positioned on the same plane as the light sources 170 and 180. It is a diagram schematically showing a cross-sectional side view of the device with added. A sample may be placed in one or more chambers in the device, light from a light source 180 may be irradiated thereto, and a light signal 181 emitted from the chamber may be detected by the light receiving unit 190 .

In the device shown in FIG. 8 , a plurality of nanostructures fixed to the inner surfaces of a substrate 100, one or more first heating chambers 111, one or more detection chambers 112, and one or more first heating chambers 111 210, a plurality of nanostructures 220 fixed to the inner surface of one or more detection chambers 112, a thermal cooler 141, one or more sample chambers 113, and a fluid channel 160 are all shown in FIGS. As described above in FIG. 7 .

In FIG. 8 , the light source 170 transmits light having a wavelength (hv ₁ ) that increases the temperature of the surrounding medium by inducing the photothermal phenomenon of the nanostructure, one or more first heating chambers 111, and/or one or more detectors. The chamber 112 may be irradiated.

The light source 180 may radiate light having a wavelength (hv ₂ ) that performs plasmon resonance absorption of the aforementioned nanostructure, maximizes scattering efficiency, or both, to the one or more detection chambers 112 .

Light incident on the detection chamber 112 by the light source 180 causes absorption or scattering by plasmon resonance absorption and/or scattering of the nanostructure 220 fixed to the inner surface of the detection chamber described above, An optical signal 181 having a wavelength (hv ₂ ′) emitted or scattered from the structure 220 may be sensed by the light receiver 190 .

Although not shown in FIG. 8 , the detection device according to the embodiment may further include one or more of the above-described polarizer, color filter, and adjusting unit, and descriptions of each of these are as described above.

FIG. 9 is a cross-sectional view of the substrate of FIG. 7 taken along line A-A′, with light sources 170 and 180 not shown in FIG. 7 facing the light sources 170 and 180 with the substrate 100 interposed therebetween. It is a diagram schematically showing a cross-sectional side view of a device to which a light receiving unit 190 located on a plane is added. As in FIG. 8 , a sample may be placed in one or more chambers in the device, light may be irradiated therefrom from a light source 180 , and a light signal 181 emitted from the chamber may be detected by the light receiving unit 190 .

In the device according to an embodiment shown in FIG. 9 , the substrate 100 , the one or more first heating chambers 111 , the one or more detection chambers 112 , the one or more first heating chambers 111 are fixed to the inner surface. a plurality of nanostructures 210, a plurality of nanostructures 220 fixed to the inner surface of one or more detection chambers 112, a thermal cooler 141, one or more sample chambers 113, a fluid channel 160, One or more light sources 170 and 180 and the light receiving unit 190 are as described in FIGS. 1 to 8 . In addition, although not shown, the device according to the embodiment may further include one or more of a polarizer, a color filter, and an adjusting unit, and each of these components is as described above.

Hereinafter, the shape of the nanostructure and the size of the nanostructure, photothermal phenomenon of the nanostructure, plasmon resonance absorption efficiency (σ _abs ), heating capacity (Q _abs ), temperature change of the surrounding medium changed by the nanostructure (ΔT), etc. explain the relationship between

The photothermal phenomenon of the nanostructure is proportional to the plasmon resonance absorption efficiency (σ _abs ) in which light having a specific wavelength irradiated from the outside is absorbed into the nanostructure. The plasmon resonance absorption efficiency (σ _abs ) may be determined by the dielectric properties of materials constituting the nanostructures, the shape of the nanostructures, and the size of the nanostructures, and may be calculated by Equation 1 below:

[Equation 1]

In Equation 1 above,

는 나노구조체의 표면을 따라 수행하는 체적분 (volume integral)을 나타낸다. k is The wavevector of the externally irradiated light, ε _m is the dielectric function of the nanostructure, ε ₀ is the dielectric function of the surrounding medium, E ₀ is the electric field by the externally irradiated light when there is no nanostructure, E is the nanostructure The size of the total electric field, including the electric field by the light irradiated from the outside and the induced electric field induced by the polarization inside the nanostructure when there is, r is the distance from the center (origin) of the nanostructure to an arbitrary point located on the space represents the position vector,

represents the volume integral performed along the surface of the nanostructure.

Therefore, those skilled in the art can adjust the plasmon resonance absorption efficiency (σ _abs ) of the nanostructure represented by Equation 1 to obtain a photothermal phenomenon in an appropriate range suitable for the purpose and use of the nanostructure. It is possible to easily select or design nanostructures capable of exhibiting this.

The heating ability (Q _abs, heating power) of the nanostructure to raise the temperature of the surrounding medium is proportional to the intensity of light irradiated from the outside. The heating ability (Q _abs ) in the steady state of the nanostructure can be calculated by Equation 2 below, and the temperature change (ΔT) of the surrounding medium changed by the nanostructure can be calculated by Equation 3 below:

[Equation 2]

In Equation 2 above,

σ _abs represents the plasmon resonance absorption efficiency of the nanostructure, I represents the intensity of the irradiated light, n represents the refractive index of the surrounding medium, c represents the speed of light , ε ₀ represents the dielectric function of the surrounding medium , E ₀ represents the electric field due to externally irradiated light;

[Equation 3]

In Equation 3 above,

는 주변 매질의 열 전달률(thermal conductivity)을 나타낸다.Q _abs represents the heating ability in the steady state of the nanostructure, R is the radius of the nanostructure,

represents the thermal conductivity of the surrounding medium.

Therefore, a person skilled in the art can determine from Equations 2 and 3 above, the heating ability of the nanostructure to be used in a steady state, and the temperature change of the surrounding medium changed by the nanostructure. (ΔT) can be easily calculated, and an appropriate nanostructure can be selected or designed from it according to the purpose and use of the nanostructure.

10 is a graph showing the plasmon resonance absorption efficiency (σ _abs ) of spherical gold nanoparticles in water, which varies depending on the size of the radius (a') of the spherical gold nanoparticles.

Referring to FIG. 10, as the radius (a′) of the spherical metal nanoparticles 230 increases, the wavelength at which the plasmon resonance absorption efficiency (σ _abs ) of the spherical metal nanoparticles 230 is maximum moves toward longer wavelengths. (red shift), the change in wavelength is very small.

FIG. 11 is a graph showing a temperature change (ΔT) of water according to plasmon resonance absorption of spherical gold nanoparticles in water, which varies depending on the size of the radius (a') of the spherical gold nanoparticles.

105K이다. 상기 온도 변화는 제1 가열 챔버(111) 혹은 검지 챔버(112) 내 나노구조체의 광열 현상을 유도하여 주변 매질의 온도를 높이는 파장(hv₁)을 조사하였을 때, 충분히 세포를 용해할 수 있는 온도, 및/또는 중합효소연쇄반응 반응으로 DNA 및/또는 RNA를 증폭시킬 수 있는 온도 변화이다. 도 10 및 도 11을 참조하면, 전술한 하나 이상의 챔버(110) 내부 표면에 고정된 복수 개의 나노구조체(200)가 전술한 구형의 금속 나노입자(230)일 경우, 플라즈몬 공명 흡수 효율(σ_abs )이 우수하며, 세포의 열 용해, 및/또는 중합효소연쇄반응으로 DNA, 및/또는 RNA를 증폭시키는 온도 변화를 발생시킬 수 있다.Referring to FIG. 11, when light having a wavelength of λ = 550 nm having an intensity of about I = 2 mW / μm ² is irradiated from the outside to spherical gold nanoparticles with a radius of 30 nm in water with a heat transfer rate of 0.58 W / mK, The temperature change of the medium water is about ΔT

It is 105K. The temperature change induces a photothermal phenomenon of the nanostructure in the first heating chamber 111 or the detection chamber 112, and when irradiated with a wavelength (hv ₁ ) that raises the temperature of the surrounding medium, the temperature at which cells can be sufficiently dissolved , and/or temperature changes that can amplify DNA and/or RNA in a polymerase chain reaction. 10 and 11, when the plurality of nanostructures 200 fixed to the inner surface of the one or more chambers 110 described above are the above-described spherical metal nanoparticles 230, the plasmon resonance absorption efficiency (σ _abs) ) is excellent, and can generate a temperature change that amplifies DNA and/or RNA by thermal lysis of cells and/or polymerase chain reaction.

12 shows the plasmon resonance absorption efficiency of core-shell nanoparticles in water depending on the radius size (a) of the silica core in core-shell nanoparticles in which the core is silica and the shell is gold with a thickness of 1 nm. It shows the result of theoretically calculating (σ _abs ).

Referring to FIG. 12 , the wavelength at which the plasmon resonance absorption efficiency (σ _abs ) is maximized varies greatly according to the change in the radial length (a) of the core of the core-shell nanoparticle 240 . In addition, if the radius length (a) of the core is increased while maintaining the thickness of the shell, the wavelength at which the plasmon resonance absorption efficiency (σ _abs ) is the maximum shifts toward the longer wavelength (red shift).

13 shows plasmon resonance absorption in water of core-shell nanoparticles in which the core is silica with a radius of 15 nm (a) and the shell is gold (Au), and the core-shell nanoparticles vary depending on the thickness of the shell. It shows the result of theoretically calculating the efficiency (σ _abs ).

Referring to FIG. 13 , the wavelength at which the plasmon resonance absorption efficiency is maximized varies greatly according to the change in the shell thickness ba of the core-shell nanoparticle 240 . In addition, if the thickness of the shell (ba) is increased while maintaining the radial length (a) of the core, the wavelength at which the plasmon resonance absorption efficiency (σ _abs ) is maximum shifts toward a shorter wavelength (blue shift).

12 and 13, the nanostructure 200 fixed inside the one or more chambers 110 described above induces a photothermal phenomenon of the nanostructures incident on the one or more chambers 110 to increase the temperature of the surrounding medium. A condition having maximum absorption efficiency for light of a wavelength (hv ₁ ), that is, a condition in which a photo-thermal effect is maximized by the absorbed light may be satisfied. A nanostructure that satisfies the above conditions may serve as the aforementioned nanoheater.

When the nanostructure 200 fixed inside the above-described one or more chambers 110 is a core-shell type nanoparticle 240 in which the core is a dielectric and the shell is a metal, the controllable plasmon resonance absorption efficiency (σ _abs ) is The range of the maximum wavelength is wide, and even a small change in the thickness of the shell (ba) and/or the radial length of the core (a) easily changes the wavelength at which the absorption efficiency (σ _abs ) according to plasmon resonance is maximized, It is possible to implement a nanoheater that is easy to maximize the heat generation efficiency by the development.

In addition, when the nanostructure 200 fixed inside the one or more chambers 110 described above is a core-shell nanoparticle 240 having a dielectric core and a metal shell, the controllable plasmon resonance absorption efficiency (σ _abs ) may be wide, and a person skilled in the art can implement a nanoantenna for easy quantitative detection of DNA and/or RNA by selecting the shape and combination as needed.

Accordingly, the detection device according to the embodiment of the present invention may be implemented as a biochip or biosensor for detection of at least one of DNA and RNA. For example, the detection may be quantitative detection.

In another embodiment, a kit for detecting at least one of DNA and RNA including a reagent reacting with a sample containing at least one of DNA and RNA and a device for detecting at least one of DNA and RNA according to one embodiment is provided. do.

Since the sample containing at least one of DNA and RNA and the reagent have been described above, detailed descriptions thereof are omitted.

The reagent may be carried in one or more chambers of the above-described detection device of one or more of DNA and RNA, and may exist separately from the detection device of one or more of DNA and RNA.

Hereinafter, a method for detecting at least one of DNA and RNA using the device for detecting at least one of DNA and RNA according to the above-described embodiment will be described.

A method for detecting at least one of DNA and RNA according to an embodiment includes one or more of DNA and RNA to be analyzed in one or more chambers formed on a substrate of the detection device according to the above-described embodiment and having a plurality of nanostructures fixed to an inner surface thereof. Put the sample containing the above, polymerase, and base; controlling the temperature of the sample by irradiating light into the chamber, thereby causing a polymerization reaction to amplify at least one of DNA and RNA including a specific sequence in at least one of the DNA and RNA; and irradiating light to the chamber where the polymerization reaction is completed, and detecting absorbed or scattered light.

FIG. 14 schematically illustrates a method of changing the temperature by adjusting the operation time of a light source and a thermal cooler according to time in order to amplify and quantitatively detect at least one of DNA and RNA in one or more chambers described above.

Referring to FIG. 14, in the method for quantitatively detecting at least one of DNA and RNA according to an embodiment, a photothermal phenomenon of the nanostructure 200 fixed to the inner surface of the at least one chamber is induced to measure the temperature of the surrounding medium. By irradiating light having a wavelength (hv ₁ ) that may be high, the internal temperature of the one or more chambers is raised to about 95 ° C., thereby separating one or more of the DNA and RNA in the one or more chambers into a single strand (Denaturation )can do.

Thereafter, light having a wavelength (hv ₁ ) capable of inducing a photothermal phenomenon of the nanostructure and increasing the temperature of the surrounding medium is turned off, and the heat cooler 141 is turned on to reduce the temperature inside the detection chamber, thereby reducing the temperature of the separated DNA and/or A single strand of RNA and a primer can be combined (Primer Annealing). The primer has a base sequence complementary to the substrate sequence of the isolated DNA and / or RNA single strand, and the reduced temperature (Tm) is the G base (guanine), C base (cytosine), A base ( adenine), and the number of T bases (thymine). The reduced temperature can be calculated by Equation 4 below. When the temperature cools down to the temperature calculated by Equation 4 below, the thermal cooler 141 can be turned off:

[Equation 4]

Tm(℃)=(4x[G+C])+(2x[A+T])

In Equation 4 above,

G is the number of guanine bases included in the primer, C is the number of cytosine bases included in the primer, A is the number of adenine bases included in the primer, and T is the number of thymine bases included in the primer.

Subsequently, the chamber is irradiated with light having a wavelength (hv ₁ ) capable of inducing a photothermal phenomenon of the nanostructure and increasing the temperature of the surrounding medium from the light source 170 to raise the temperature of the inside of the chamber to the activation temperature of the DNA polymerase. can

A DNA double helix can be synthesized (Polymerization) by activating DNA polymerase to sequentially polymerize DNA and/or RNA single strands and complementary dNTP bases at the 3' end of a primer bound to DNA and/or RNA single strands. .

The DNA polymerase may be, for example, Taq polymerase, and in the case of Taq polymerase, the activation temperature may be about 72°C. The time for maintaining the activity temperature of the polymerase in the chamber may be appropriately selected depending on the length of a specific sequence in DNA and/or RNA to be amplified and the polymerization rate of the DNA polymerase.

The wavelength of light having a wavelength (hv ₁ ) capable of increasing the temperature of the surrounding medium by inducing the photothermal phenomenon of the nanostructure, the On / Off cycle of the light source, and the On / Off of the thermal cooler 141 The time to maintain the active temperature of the polymerase may be controlled by adjusting the cycle.

Denaturing the DNA and/or RNA into single strands; binding (Primer Annealing) the separated DNA and/or RNA single strand and a primer; One cycle of synthesizing (polymerization) a DNA double helix by sequentially polymerizing dNTP bases complementary to DNA and/or RNA single strands to the 3' end of a primer linked to DNA and/or RNA single strands with DNA polymerase. It can be a cycle (1 cycle), and the 1 cycle cycle can be repeated a plurality of times.

At least one chamber in which a plurality of nanostructures are fixed on the inner surface is irradiated with light having a wavelength (hv ₂ ) that absorbs the plasmon resonance of the nanostructures, maximizes scattering efficiency, or performs both, from which After the irradiated light is absorbed by plasmon resonance absorption and/or scattering of the nanostructure, the emitted or scattered wavelength (hv ₂ ') is sensed by the light receiving unit 190 to detect amplified DNA. . The detection may be quantitative detection.

For each of the one cycle cycle or the plurality of cycle cycles, plasmon resonance absorption of the nanostructures, maximization of scattering efficiency, or both are performed from the light source 180 to one or more chambers in which a plurality of nanostructures are fixed on the inner surface. After irradiating light of a wavelength (hv ₂ ) of the nanostructure, plasmon resonance absorption of the nanostructure, or a reflected or transmitted light signal of the light remaining after being absorbed by the scattering phenomenon, and / or the nanostructure causes plasmon resonance absorption, The light signal 181 emitted or scattered therefrom can be sensed by the light receiving unit 190, and the amplified DNA can be detected for each cycle cycle or a plurality of cycle cycles. The detection may be quantitative detection.

Although not shown in the drawing, when the sample is a cell, a cell suspension, or a cell nucleus, before polymerizing at least one of DNA and RNA containing a specific sequence in at least one of the DNA and RNA, formed on the above-described substrate and irradiates light to one or more chambers in which a plurality of nanostructures are fixed on the inner surface to adjust the temperature of the sample, thereby thermally dissolving the sample by the heat generated by the photothermal phenomenon of the plurality of nanostructures 200 Further steps may be included. After the sample is thermally dissolved, the method for detecting DNA and/or RNA present therein is as described above.

Although the embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements made by those skilled in the art using the basic concept of the present invention defined in the following claims are also included in the scope of the present invention. that fall within the scope of the right.

100: substrate
110: one chamber
111: first heating chamber
112: detection chamber
113: sample chamber
114: reagent chamber
141: heat cooler
160: fluid channel
170, 180: light source
181: light signal emitted or scattered therefrom after the nanostructure causes plasmon resonance absorption
190: light receiving unit
200, 210, 220: nanostructure
230: spherical metal nanoparticles
240: Core-shell nanoparticles in which the core is dielectric and the shell is metal
a: Radial length of the core of the core-shell nanoparticle
b: Radial length of the entire nanoparticle including the core and shell of the core-shell type nanoparticle
a': length of the radius of the spherical nanoparticle

Claims (20)

Board;
one or more chambers formed over the substrate;
a plurality of nanostructures fixed to an inner surface of the one or more chambers;
one or more light sources providing incident light to the one or more chambers; and
A light receiving unit for detecting a light signal emitted from the nanostructure,
The plurality of nanostructures operate as nanoheaters or nanoantennas,
The one or more chambers,
one or more first heating chambers;
one or more detection chambers; and
one or more fluid channels connecting between the one or more first heating chambers and the one or more detection chambers; Including,
The detection device of at least one of DNA and RNA, wherein the at least one first heating chamber has an inner surface having a reflection coating. According to claim 1,
In the at least one first heating chamber, the plurality of nanostructures operate as the nanoheater,
Wherein the one or more detection chambers detect at least one of DNA and RNA in which the plurality of nanostructures operate as the nanoheater or the nanoantenna. According to claim 1,
the one or more chambers further comprising one or more sample chambers;
The detection device of at least one of DNA and RNA, further comprising at least one fluidic channel connecting between the at least one sample chamber and the at least one first heating chamber. According to claim 3,
the one or more chambers further comprising one or more reagent chambers;
The device for detecting at least one of DNA and RNA further comprising at least one fluidic channel connecting between the at least one reagent chamber and the at least one first heating chamber. According to claim 1,
Wherein the substrate is a transparent glass or polymer having a refractive index of 1.3 to 1.9, detecting at least one of DNA and RNA. According to claim 5,
The glass includes any one selected from SiO ₂ , BK7, SF10, SF11, N-LASF46A, and combinations thereof,
The polymer is a polystyrene-based polymer, a polymethyl methacrylate polymer, a polycarbonate-based polymer, a cyclic olefin copolymer, or a detection device of at least one of DNA and RNA including a combination thereof. According to claim 1,
The nanostructure is a spherical metal nanoparticle,
Core-shell nanoparticles in which the core is dielectric and the shell is metal,
or a combination thereof, wherein at least one of DNA and RNA is detected. According to claim 7,
In the spherical metal nanoparticles, the metal is gold (Au), silver (Ag), copper (Cu), aluminum (Al), or a combination thereof, wherein at least one of DNA and RNA is detected. According to claim 7,
In a core-shell nanoparticle in which the core is a dielectric and the shell is a metal,
The dielectric is SiO ₂ , BK7, SF10, SF11, N-LASF46A, A polystyrene-based polymer, a polymethylmethacrylate polymer, a polycarbonate-based polymer, a cyclic olefin copolymer, or a combination thereof,
Wherein the metal includes gold (Au), silver (Ag), copper (Cu), aluminum (Al), or a combination thereof. According to claim 7,
The nanostructure has a size of 1 nm to 1000 nm, and at least one detection device of DNA and RNA. According to claim 1,
The one or more chambers may include one or more waste chambers; and
The detection device of one or more of DNA and RNA further comprising one or more fluidic channels connecting the one or more waste chambers and the one or more detection chambers. According to claim 1,
The detection device of at least one of DNA and RNA, wherein the one or more detection chambers include one or more thermal coolers. According to claim 1,
At least one of the one or more light sources detects one or more of DNA and RNA for irradiating light with a wavelength in the range of 10 nm to 10 μm. According to claim 1,
The one or more light sources include light having a wavelength that independently induces a photothermal phenomenon of the nanostructure to increase the temperature of a surrounding medium;
Light having a wavelength that performs plasmon resonance absorption of the nanostructure, maximizes scattering efficiency, or both, or
At least one detection device of DNA and RNA irradiating light having a wavelength that induces a photothermal phenomenon of the nanostructure to increase the temperature of the surrounding medium and absorbs plasmon resonance of the nanostructure, maximizes scattering efficiency, or performs both. . According to claim 1,
Wherein the at least one light source emits monochromatic or polychromatic light, each independently, at least one of DNA and RNA. According to claim 1,
The device for detecting at least one of DNA and RNA, further comprising at least one polarizer, color filter, or combination thereof between the substrate and the at least one light source. According to claim 1,
The light receiving portion is on the same plane as the one or more light sources with respect to the substrate, or
A detection device for at least one of DNA and RNA present on a surface facing the at least one light source, centering on the substrate. According to claim 1,
The detection device of at least one of DNA and RNA, further comprising a control unit for controlling a wavelength of light incident on the at least one chamber and an on/off cycle of the at least one light source. A device for detecting at least one of DNA and RNA according to any one of claims 1 to 18, and
A kit comprising a reagent that reacts with a sample containing at least one of DNA and RNA. Of the detection device of at least one of DNA and RNA according to any one of claims 1 to 18
Putting a sample containing at least one of DNA and RNA to be analyzed, a polymerase, and a base into one or more chambers formed on a substrate and having a plurality of nanostructures fixed to an inner surface thereof;
controlling the temperature of the sample by irradiating light into the chamber, thereby causing a polymerization reaction to amplify at least one of DNA and RNA including a specific sequence in at least one of the DNA and RNA;
A method for detecting at least one of DNA and RNA containing a specific sequence, comprising irradiating light to a chamber in which the polymerization reaction is completed and detecting an absorbed or scattered light signal.

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