KR102544040B1 - Method for Controlling Fluid Flow Using Enzyme Reaction and Diagnosis Chip Using the Method - Google Patents

Abstract

The present invention relates to a flow rate control method capable of accelerating the flow rate of a specific section in a paper chip made of one sheet and to a paper chip to which the method is applied, and more particularly, to a paper chip having a channel through which a fluid moves, the channel The present invention relates to a method for controlling a local flow rate in a paper chip, characterized in that the flow rate of lateral movement of a fluid is increased by a reaction between glucose oxidase and glucose in a specific section of and a paper chip to which the method is applied.

Description

Method for controlling fluid flow rate using enzyme reaction and using diagnostic kit using the same {Method for Controlling Fluid Flow Using Enzyme Reaction and Diagnosis Chip Using the Method}

The present invention relates to a flow rate control method capable of accelerating the flow rate of a specific section in a paper chip made of one sheet, and to a paper chip to which the method is applied.

The increase in infectious diseases requires rapid and accurate disease diagnosis. Methods capable of directly detecting biomarkers from a sample solution without performing a complicated process in a specialized laboratory have been developed and play an important role in the diagnosis of bacterial, viral, fungal or parasitic infectious diseases. However, most diagnostics still require the use of expensive analytical instruments and specialists to perform sequential analysis steps. In addition, as the relative ratio of the working age group decreases with the entry of an aging society and the population of the elderly group with high disease incidence increases, the cost of medical diagnosis is emerging as a serious problem. In particular, developing countries that do not have adequate medical insurance benefits are struggling with expensive equipment and limited access to skilled experts, in addition to the cost of individual disease diagnosis. In order to solve these problems, low-cost diagnostic equipment capable of diagnosing diseases simply at a low cost at a medical institution or home without professional personnel or special devices is required.

ELISA (Enzyme-linked immunosorbent assay) is one of the most widely used analysis methods for detecting a target substance in a sample. ELISA protocols include repetitive experimental steps such as sample incubation, labeling, washing, and signal amplification in multiple pipetting protocols performed manually or by automated robotic systems. To minimize these labor-intensive protocols, dipsticks or lateral flow tests (LFTs) are being developed as alternatives as a quick and simple diagnostic format. These methods have advantages in terms of simplicity, closeness, and portability, but automated sequential analysis such as multi-step biochemical reactions, which are unavoidable technical problems, is difficult to implement.

Recently, paper-based microfluidic systems have emerged as promising analytical and diagnostic platforms. Leading research groups use soluble sugars or polymers on paper substrates, movable arms, layer sliding, electroosmotic pumps, stacking and externally activating valves to move samples and reagents in a specific order into the detection zone. It showed the possibility of fluid movement. By combining the sequential fluid transfer function with paper chips, continuous biochemical reactions can be performed in an automated multi-step manner.

Although this approach enables multi-step reactions in paper chips, it still has some limitations. First, it may interfere with the analysis. Adding chemicals to the paper changes the porosity of the paper matrix and its permeability, which controls the flow rate of the fluid. Therefore, when the sample solution is loaded, the chemical substance is melted or permanently buried in the paper substrate, so that the flow rate can be controlled. However, just as the addition of sugar interferes with glucose-related assays, the addition of sugar or chemicals such as polymers or surfactants can limit the type of target analyte that can be measured. Second, user intervention is required. Sliding of a movable arm or layer operated by a user can transfer a plurality of samples and reagents simultaneously by connecting a fluid channel to another channel. However, for precise analysis results, manual operation must be performed by specialized personnel rather than general users. Third, the manufacturing process becomes complicated. An electrically actuated valve may be used to connect or close the fluid channel. However, a controller, voltage supply, and power source are required to activate the valve. Automated operation enables more precise manipulation than manually operated structures, but since these parts must be stacked and integrated in a complex multi-step process, the manufacturing process is complicated and the price rises accordingly. Fourth, it requires external equipment. Many recent μPADs require an external analyzer to analyze the intensity of color change of reagents as a result of multi-step analysis.

In order to solve this problem, the key is to develop a method for controlling the flow rate in paper that can sequentially move fluids without user intervention without using additional chemicals or external equipment that can interfere with the analysis. At the same time, the paper-based format used to perform the multi-step analysis achieved by automated sequential fluid transfer must be able to be manufactured in a simple and inexpensive way, and no additional equipment is required to detect the analytical results. Creating a new paper-based analytical format that can simultaneously meet all these important needs is worth the challenge.

The present inventors proposed a 3D microfluidic paper based analytical device (3D-μPAD) capable of quantifying a target material by digital analysis without using external equipment in Patent Registration No. 10-1493051 did In the registered patent, the concentration of the target material can be simply quantified by counting the number of bars whose color has changed to blue. The 3D-μPAD can be simply manufactured by printing wax patterns on both sides of a sheet of paper and heat-treating for several tens of seconds.

Another patent of the present inventors, Registered Patent No. 10-1662802, proposed a method of adjusting the flow rate by controlling the height of the channel using wax. The above patent showed that different color waxes with different rheological characteristics can be simultaneously printed to form channels of different heights and lengths at once, and using this, a 3D- μPAD has been presented.

These 3D-μPADs allow for instrument-free analysis, simplifying the manufacturing process by reducing manufacturing time and material use, but controlling automated sequential fluid movement by controlling flow rates without the use of chemicals or external equipment. However, 3D-μPADs capable of carrying out multi-step reactions are still attracting much attention.

Registered Patent No. 10-1662802 Registered Patent No. 10-1493051

An object of the present invention is to provide a novel flow rate control method capable of adjusting the flow rate in a specific section so that a sequential reaction is possible in a paper chip made of one sheet of paper.

Another object of the present invention is to provide a paper chip having a flow rate control panel to which the flow rate control method is applied.

Another object of the present invention is to provide a paper chip capable of simply quantifying glucose content without user intervention or separate equipment.

The present invention for achieving the above object is a paper chip having a channel through which fluid moves, characterized in that the flow rate of lateral movement of the fluid is increased by a reaction between glucose oxidase and glucose in a specific section of the channel. It relates to a method for controlling the local flow rate in

The present invention is to confirm that the change in fluid composition by glucose oxidation in a paper chip increases the flow rate of the fluid in the channel, and to use this to control the local flow rate in the paper chip. The paper chip is the most useful model as a low-cost diagnostic kit because it is easy to supply and demand raw materials, it can be mass-produced in a roll-to-roll method, and above all, it uses low-cost raw materials. If the flow rate can be controlled in a specific section of the channel constituting the paper chip, a complex sequential reaction is possible within the paper chip. Glucose is oxidized in water to produce gluconolactone and gluconic acid. As a result of dissolving glucose, gluconolactone, and gluconic acid at the same concentration, and measuring the flow rate in a paper chip of the same shape, the flow rate of the water and glucose solution was almost the same, but compared to the glucose solution, the gluconolactone solution and the gluconic acid The flow rate of the acid solution increased significantly. This change in flow rate is expected to be due to the increase in pores of the cellulose constituting the paper chip by gluconolactone or gluconic acid.

The glucose oxidation reaction in paper chips can be mediated by, for example, glucose oxidase, and the increase in flow rate was proportional to the glucose concentration.

Therefore, the degree of increase in flow rate in a specific channel can be controlled by the concentration of glucose.

The present invention also applies the local flow rate control method to a paper chip having a flow rate control channel having a flow rate different from other channels by automatically adjusting the flow rate, and the flow rate is accelerated by the oxidation reaction of glucose in the flow rate control channel It relates to paper chips characterized by In the paper chip of the present invention, flow rate control channels may be formed in a plurality of sections, and the degree of acceleration of the fluid may be varied in the flow rate control channels of the plurality of sections according to a difference in the concentration of glucose oxidized in the flow rate control channels. "Multiple sections" means two or more sections, and it is meaningless to limit the number. cannot be controlled by differences in concentration. Therefore, it is natural that two or more paths must exist between corresponding sections in order to differentiate the degree of acceleration of the flow rate control channel in a plurality of sections due to the difference in glucose concentration.

When the paper chip is placed on the floor during sample analysis, the flow of the fluid may be interfered with by contact with the floor surface. In order to prevent interference, it is preferable that the paper chip has a folding portion that can be folded and used as a leg at the outer end (see FIG. 7). The folded portion may be formed on the entire outer periphery or may be formed on a part of the surface, but it is natural that it should not affect the lateral movement of the fluid in the paper chip.

In order for the glucose oxidation reaction to occur in the flow control channel, (a) glucose or (b) glucose oxidase is adsorbed to the flow control channel, and in the case of (a), glucose oxidase is adsorbed at the front end of the flow control channel , (b) may have a structure in which glucose is adsorbed. As the sample is loaded on the paper chip having the configuration of (a) and the fluid moves, the glucose oxidase adsorbed at the front end of the flow control channel moves with the fluid, and when it reaches the flow control channel, oxidation of the adsorbed glucose Inducing a reaction, the flow rate is accelerated. In the case of (b), as the fluid moves, the glucose adsorbed on the shear moves along with the fluid, and when it reaches the flow control channel, an oxidation reaction occurs by the adsorbed glucose oxidase. In this specification, the "front end" of the flow control channel may be directly in front of the flow control channel, but it does not matter if it is located at a certain distance. As described above, a position where the material adsorbed on the front end can move with the fluid and pass through the flow control channel is sufficient.

The paper chip of the present invention as described above can be usefully used for the analysis of glucose adsorbed on the paper chip or a sample unaffected by glucose oxidase. Fluid acceleration can be applied in various ways in paper chips, for example, it can be used to shorten the analysis time in paper chips simply by lateral flow, or by flowing channels of different paths at different flow rates. It can also be used to proceed sequential reactions in paper chips.

If a sample containing (a') glucose oxidase or (b') glucose is analyzed, only glucose in the case of (a') and glucose oxidase in the case of (b') are added to the flow control channel of the paper chip. By adsorbing, an accelerating effect of the fluid can be obtained.

In particular, the paper chip of the present invention can be used for analysis of glucose or glucose oxidase. Since the moving speed of the fluid is proportional to the moving distance in a predetermined time, it can be used to qualitatively analyze the presence or absence of glucose or glucose oxidase in a sample. Furthermore, since the degree of acceleration of the fluid depends on the concentration of glucose, quantitative analysis is also possible by measuring the distance of the fluid moving up to a predetermined time after loading the sample.

Specifically, the present invention is a sample pad on which the sample is loaded; a reaction channel connected to the sample pad and adsorbed with glucose oxidase; A detection channel for detecting the fluid passing through the reaction channel; and providing a paper chip for analyzing glucose content, characterized in that the glucose content in the sample is analyzed by the movement distance of the fluid moving for a predetermined time after loading the sample. . In the above, the detection channel can detect the movement of the fluid at the distal end of the channel so that the change in flow rate derived from the reaction channel is sufficiently reflected, and an absorption pad for absorbing the fluid passing through the detection channel is included as an additional component. You may. In the paper chip of the present invention, since the degree of acceleration of the fluid increases as the content of glucose in the sample increases, the moving distance of the fluid moving during the same time reflects the content of glucose in the sample. Therefore, the glucose content in the sample can be measured by the moving distance. According to the configuration, the amount of glucose in the sample can be calculated simply by checking the location of the fluid after a predetermined time without any other intervention after the user loads a predetermined amount of the sample onto the sample pad.

In order to check the amount of glucose, it is preferable that a marker for measuring the detection position of the fluid is marked on the detection channel of the paper chip. The marker may be, for example, a scale indicating a specific interval or may be in the form of a digital dot as shown in FIG. 2 . Also, the markers may have equal intervals, or may have a shape in which the intervals are gradually narrowed or widened in consideration of the concentration. Regardless of its shape, it can be confirmed as a marker at the final detection position of the fluid, so the moving distance of the fluid can be converted into numerical values without a measuring tool such as a separate ruler.

More conveniently, it is preferable that the paper chip displays the glucose concentration for each detection position. With the above configuration, the glucose concentration in the sample can be intuitively derived from the detection position identified through the marker used without calculation by a specific formula.

Identification of the fluid in the detection area can be achieved by confirming the boundary of the wet part of the detection area of the paper chip. In order to increase discrimination, it is preferable that the fluid is marked so that the identification of the fluid is easy. The labeling of the fluid is simple, for example, by adding color-indicating ink to the sample solution, or by adsorbing ink to a specific point on a paper chip as shown in FIG. It can be made to show color. Alternatively, the color change of TMB using hydrogen peroxide produced by glucose oxidation can be used. To this end, the paper chip of the present invention further includes a second reaction channel and a movement channel, which diverge from the reaction channel and join at the detection channel, between the reaction channel and the detection channel, and the second reaction channel has gold ions. is adsorbed, and TMB (3,3',5,5'-tetramethylbenzidine) is adsorbed to the detection channel. With the above configuration, as shown in FIG. 4, the glucose oxidation reaction (first reaction), the gold nano ion generation reaction (second reaction), and the TMB oxidation reaction (third reaction) proceed sequentially, so that colorless TMB turns yellow. Oxidized TMB is generated, so the color of the fluid changes to yellow, making it easier and more accurate to check the moving distance.

The second reaction channel may be configured as a delay channel relative to the transfer channel to give sufficient time for the gold nano ion generation reaction. At this time, the delay passage may be constructed using a conventional method of forming the delay passage. For example, the delay passage may be configured by making the length or width of the second reaction channel different from that of the movement passage, and the delay passage may be constructed by adjusting the height using wax as in the embodiment.

As described above, according to the flow rate control method of the present invention, the movement of the fluid can be selectively accelerated in a specific section without user intervention by a simple method without using expensive raw materials by using the acceleration phenomenon caused by the enzyme reaction, It can be usefully used in the manufacture of paper chips capable of sequential reactions.

In addition, the paper chip for analyzing the glucose content of the present invention can be economically mass-produced, and can quantitatively evaluate the concentration of glucose in a sample by a simple method without professional training without an external detection device by using the acceleration phenomenon by the enzyme reaction. It is useful as a low-cost on-site diagnostic kit.

1 is a schematic diagram of a paper chip according to an embodiment of the present invention.
Figure 2 is a photograph and graph of a paper chip showing that the flow rate of the fluid is accelerated by the glucose oxidation reaction in the paper chip.
Figure 3 is a graph showing the flow rate difference of the main products of the glucose oxidation reaction.
Figure 4 is a sequential reaction formula for signal enhancement of glucose oxidation reaction.
5 is a schematic diagram of a paper chip according to another embodiment of the present invention.
Figure 6 is a photograph and graph showing the results of glucose quantification by the paper chip of Figure 5.
7 is a photograph showing an example of a foldable paper chip.

Hereinafter, the present invention will be described in more detail with reference to the attached examples. However, these embodiments are only examples for easily explaining the content and scope of the technical idea of the present invention, and thereby the technical scope of the present invention is not limited or changed. It will be obvious to those skilled in the art that various modifications and changes are possible within the scope of the technical spirit of the present invention based on these examples.

[Example]

Example 1: Evaluation of change in flow rate by glucose oxidase in paper chips

It was confirmed whether the glucose oxidase (GOx, Glucose Oxidase) adsorbed on the channel of the paper chip could change the flow rate of the glucose solution.

As shown in (a) of FIG. 1, the front and back sides of the paper chip were designed, and both sides were printed with a wax print so that the designed color was printed. During double-sided printing, the red design surface was made symmetrical, and then heat treatment was performed at 140 ° C. for 0.52 seconds to infiltrate the paper with wax to prepare paper chips.

Figure 1 (b) shows the three-dimensional structure of the flow path of the paper chip manufactured by the above method. As can be seen in (b) of FIG. 1, the paper chip has a sample pad, a reaction channel connected to the sample pad to react with glucose in the sample and GOx adsorbed to the channel, a moving channel branched from the reaction channel and moving fluid, It is composed of a delay channel branching from the reaction channel to delay movement of the fluid, a detection channel in which the fluid passing through the movement channel and the delay channel is merged and detected, and an absorption pad for absorbing the fluid passing through the detection channel. The detection channel was designed to enable digital analysis of the difference in flow rate by simple counting, and 10 μl of 1 mg/mL glucose oxidase (Sigma-Aldrich) aqueous solution was pre-adsorbed on the reaction channel and dried at room temperature.

200 μl of a glucose solution having a concentration of 0 to 5 mg/mL was loaded onto the sample pad while photographing the paper chip with a CCD camera, and then the movement of the fluid was observed. Figure 2 (a) is an image capture picture of the CCD measurement result at a specific time after loading the sample. In addition, from the observation results, the time to pass through the reaction channel, movement channel, and delay channel was measured as the time t ₁ , t ₂ , and t ₃ respectively to reach the point indicated in (b) of FIG. 1, and the time to reach the detection channel Measured by t ₄ , it is shown in (b) and (c) of FIG. 2 .

It can be seen from FIG. 2 that as the concentration of glucose increases, the movement of the fluid becomes faster, and accordingly, the time to reach a specific point is shortened. In (a) of FIG. 2, the black line displayed on the image indicates the front point of the fluid. The drawing on the right of (a), in which the detection area is enlarged from the captured image of 19.5 minutes, shows that the location where the fluid reaches the detection area is different depending on the glucose concentration in the solution, and it can be simply counted by the number of digital dots wet with the fluid. show That is, as the glucose concentration in the solution increases from 0 to 5.0 mg/mL, the digital analysis result increases from 0 to 4, so that the glucose concentration in the solution can be simply analyzed with the naked eye. Specifically, as the glucose concentration increased, the time t ₁ required to pass through the reaction channel gradually decreased. As t ₁ increased, t ₂ , t ₃ and t ₄ also increased as the glucose concentration increased. The difference between t ₁ when the glucose concentration was 0 and 5 mg/mL was less than 1 minute, but the difference between t ₂ and t ₃ was about 5 minutes and t ₄ was about 10 minutes, and the gap gradually increased. This suggests that the flow rate is not simply changed by the interaction between GOx adsorbed on the reaction passage and glucose in the sample, but that the change in the composition of the sample causes the change in flow rate.

Example 2: Analysis of causes of flow rate change

Glucose is oxidized by glucose oxidase to produce equivalents of hydrogen peroxide and gluconolactone. The resulting gluconolactone is partially hydrolyzed in water and exists in equilibrium with gluconic acid.

In order to confirm the main cause of the acceleration due to the reaction with glucose oxidase in solutions containing glucose, the viscosities of water, 56 µM glucose, 56 µM gluconolactone, and 56 µM gluconic acid solutions were measured using a viscometer (Brookfield DV-11+). Pro, MA, USA). At 23°C and 30% relative humidity, the average viscosity of each solution was 0.8±0.2 cP, which was almost the same. Therefore, it was confirmed that there was no significant change in viscosity during the glucose oxidation reaction.

Accordingly, the flow rate was measured by the time taken to reach the detection channel after each solution was loaded using a paper chip having no reaction channel (glucose oxidase was not adsorbed). 3 shows the results, the gluconic acid solution reached within 9 minutes and had the fastest flow rate, and the gluconolactone solution also reached within about 9.2 minutes. On the other hand, the water and glucose solutions reached 10.6 and 10.7 minutes, respectively, although their viscosities were similar, indicating that a difference in flow rate occurred depending on the composition change after the glucose oxidation reaction.

This difference in flow rate is considered to be due to the fact that the cellulose constituting the paper is decomposed by H ⁺ ions derived from gluconic acid, thereby increasing the pore diameter of the paper channel.

Example 3: Signal-enhanced glucose analysis paper chip

As in Example 1, even if there is no separate signal enhancement for detection, the concentration of glucose can be measured without additional equipment, but signal enhancement may be required to enable more clear detection. As described in the examples, glucose oxidase reacts with glucose to produce an equivalent amount of hydrogen peroxide. Using the sequential reaction of FIG. 4, the signal can be amplified by the generated hydrogen peroxide. Specifically, when an equivalent amount of hydrogen peroxide is generated by a reaction between glucose and glucose (first reaction), gold ions are reduced to gold nanoparticles (AuNPs) using a part of the generated hydrogen peroxide (second reaction). The remaining hydrogen peroxide oxidizes colorless TMB (3,3',5,5'-tetramethylbenzidine) to yellow oxidized TMB by the nanozyme action of the reduced gold nanoparticles (third reaction). As confirmed in Example 1, in this paper chip, the higher the concentration of glucose in the sample, the faster it reaches the detection channel, so when observed after a predetermined time has elapsed from sample loading, the color changes due to the oxidation of TMB in the detection channel. The number of digital dots changed reflects the amount of glucose in the sample.

However, for the third reaction, sufficient reaction time must be given to generate gold nanoparticles in the second reaction channel, and hydrogen peroxide from the first reaction is supplied to the third reaction channel before gold nanoparticles are supplied by the second reaction. It should be.

The front and back sides of the designed paper chip were printed on both sides as shown in FIG. During double-sided printing, the red design surface was made symmetrical, and then heat treatment was performed at 140 ° C. for 0.52 seconds to infiltrate the paper with wax to prepare paper chips.

5 (b) shows the three-dimensional structure of the flow path of the paper chip manufactured by the above method. The paper chip is connected to the sample pad and the sample pad, and the reaction between glucose in the sample and GOx adsorbed on the channel occurs. 1 reaction channel, a second reaction channel (delay channel) branching from the reaction channel and moving the fluid with a delay, a moving channel branching from the reaction channel to the second reaction channel and moving the fluid, passing through the moving channel and the second reaction channel It consists of a third reaction channel into which the fluid is joined and an absorption pad that absorbs the fluid passing through the third reaction channel. In this paper chip, the third reaction channel serves as a detection channel because the third sequential reaction occurs and the detection result is displayed at the same time. That is, in the paper chip of FIG. 1, the reaction channel, the delay channel, and the detection channel are respectively referred to as the first reaction channel, the second reaction channel, and the third reaction channel so as to show sequential reactions in the present paper chip, and the design shape is somewhat Although different, the basic channels constituting the paper chip are the same as those of the paper chip of FIG. 1 . For the sequential reaction on the paper chip, 8 μl of 1 mg/mL glucose oxidase aqueous solution was added to the first reaction channel, 14 μl of 5 mg/mL HAuCl ₄ aqueous solution was added to the second reaction channel, and 1.5 μl of 99% TMB solution was added to each digital dot and dried at room temperature.

When a sample containing glucose is added to the sample pad, the sequential reaction described in FIG. 4 proceeds while sequentially moving along the channel of the paper chip. The generation of gold nanoparticles in the second reaction channel was confirmed by analyzing the third reaction channel with an EDX-equipped SEM image (data not shown). When AuNPs, the product of the second reaction channel, and hydrogen peroxide, the product of the first reaction channel, are supplied in time to the third reaction channel, which is the detection channel, along the second reaction channel and the migration channel, respectively, in the presence of hydrogen peroxide, the AuNPs are nanozymes It oxidizes colorless TMB to yellow oxidized TMB. Therefore, the number of yellow-colored digital dots of a specific time detection channel after sample loading will be proportional to the amount of glucose in the sample.

To confirm this, 180 μl of glucose at a concentration of 0 to 10 mg/mL was loaded onto the paper chip sample pad shown in FIG. 5, and the yellowing of the detection area was counted. FIG. 6 shows a photograph of a paper chip and a counting result graph after 20 minutes of loading, showing that the number of yellow dots is actually proportional to the glucose concentration and the reproducibility is excellent.

In the experiment, the paper chips were measured while placed on the holder to exclude interference caused by contact with the bottom surface, or were used in the form of foldable paper chips as shown in FIG. 7 .

Claims (14)

In the local flow rate control method in a paper chip that controls the flow rate in a specific section so that sequential reactions are possible in a paper chip made of one sheet of paper,
The control of the flow rate is to increase the lateral movement flow rate of the fluid by the oxidation reaction of glucose.
The method of claim 1,
The local flow rate control method in a paper chip, characterized in that the oxidation reaction of glucose is by glucose oxidase.
According to claim 1 or claim 2,
A method for controlling the local flow rate in a paper chip, characterized in that the degree of increase in flow rate is controlled by the concentration of glucose.
In a paper chip in which the flow rate in a specific section is controlled so that sequential reactions are possible in a paper chip made of one sheet of paper,
The flow rate control channel through which the flow rate is controlled is a paper chip, characterized in that the flow rate is accelerated by the oxidation reaction of glucose.
The method of claim 4,
The flow rate control channel is formed in a plurality of sections,
The paper chip, characterized in that the degree of acceleration of the fluid in the plurality of sections of the flow control channel is different due to the difference in the concentration of glucose oxidized in the flow control channel.
According to claim 4 or claim 5,
The paper chip is characterized in that it has a folding portion that can be used as a leg folded at the outer end of the paper chip.
According to claim 4 or claim 5,
(a) glucose or (b) glucose oxidase is adsorbed to the flow control channel,
Paper chip, characterized in that in the front end of the flow control channel, glucose oxidase in the case of (a) and glucose in the case of (b) are adsorbed.
According to claim 4 or claim 5,
(a) glucose or (b) glucose oxidase is adsorbed to the flow control channel,
A paper chip characterized in that the sample loaded on the sample pad contains glucose oxidase in the case of (a') and glucose in the case of (b').
A sample pad on which a sample is loaded;
a reaction channel connected to the sample pad and adsorbed with glucose oxidase;
Including; a detection channel in which the fluid passing through the reaction channel is detected,
A paper chip for analyzing glucose content, characterized in that the glucose content in the sample is analyzed by the moving distance of the fluid moving for a predetermined time after loading the sample.
The method of claim 9,
A paper chip for glucose content analysis, characterized in that the detection channel of the paper chip is marked with a marker for measuring the detection position of the fluid.
The method of claim 9,
A paper chip for analyzing glucose content, characterized in that the glucose concentration for each detection position is described in the detection channel of the paper chip.
According to any one of claims 9 to 11,
A paper chip for analyzing glucose content, characterized in that the fluid is labeled for easy identification of the fluid.
The method of claim 12,
Between the reaction channel and the detection channel,
Further comprising a second reaction channel and a movement channel that diverge from the reaction channel and then join the detection channel,
Gold ions are adsorbed in the second reaction channel,
Paper chip for analyzing glucose content, characterized in that TMB (3,3 ', 5,5'-tetramethylbenzidine) is adsorbed in the detection channel.
The method of claim 13,
The second reaction channel is a paper chip for analyzing glucose content, characterized in that composed of a delay flow path relative to the movement channel.

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